BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 285/502 (56.77%), Postives = 367/502 (73.11%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPE--QYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK 511
            Y ++ ++++  ++ + +PLTLSEK+LYSHL D   ++   RG+ Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLIQA+   KKDL RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYAFPG +M+GTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IGV+LTGKLSGWT+PKDVILKVA ILT KGGTGAIVEYFG G  ++SCTG GTICNMGAEIGATTS F Y+  M+ YL +T+R  +A+ A+K +       EV  +P+    +DQV++I+L+ L P +NGPFTPDL+T +G E+ EKA KN WP++V+  LIGSCTNSSYED++R ++I +     G+K KSN  I PGSE IR T ERDG+       G  +  NACGPCIGQW R +  K   KN+IV S+NRNF  R D NP TH FV SPE+T  ++ AG LDF+P +D +    G E+K N P G ELP +
Sbjct:  628 YEKLVQKLEIVKDRLNRPLTLSEKVLYSHL-DDPKNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIQAQIEGKKDLARAKDLNAEVYNFLSTSGAKYGVGFWKPGSGIIHQIILENYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRSDIANLADKYR-------EVLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPDLATPIG-ELGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAIIKQALAKGMKSKSNFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWDRQNITK-GEKNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQ 1119          

HSP 2 Score: 53.1434 bits (126), Expect = 2.566e-7
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 0

Query:  648 EADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            +ADYD I+ DD     D+ +FAP + +   + H+DGS   I LNH++N+ QI
Sbjct: 1171 KADYDKIRPDDLITLNDLVNFAPGRPVVATIKHSDGSSENISLNHSFNEQQI 1222          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 131.339 bits (329), Expect = 9.226e-32
Identity = 164/575 (28.52%), Postives = 239/575 (41.57%), Query Frame = 0

Query:   42 WDGMPSKAFTRGKDY-VDFAPDRIACQDATAQMALLQFMQAGKDKV------------AVPTTVHCDHLIQAKQGAKKDLKRANE-----VSNEVFDFLG-SVSNKYGIGFWAPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANP--EQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDW----------------------------PMKVEWSL---------IGSCTNSSYEDLSR-ASSIAEFGQKNGLKLKSNL--GINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKN------SIVHSFNRNFAKRADGNPNTHA-FVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGW-ELPPKDSEMQGVK-------LLIKAYGKCTT 530
            W+G       RGK+  + F P R+  QD T   A++ F  A +D V              PT +  DH IQ     +      NE      + E F FL    S+   +    PG+GI+HQV LE  A          FP  + +GTDSHT    GLG+V  GVGG +A  VM G    +  P+++G R++G         D++L + + L   G  G  VE++GPG   +S   + TI NM  E GAT   F  D     YLR T+RE+      +   HLT     Y +   +  F Q++ ++L  +    +GP  P     V T+M     KND+                            P   E+ L         I SCTN+S   +   A  +A+     GL +   +   ++PGS ++ +  +  G++   + LG +I       CIG     +D      N      + V S NRNF  R   NP+T A ++ASP L    A AGR+D +   + L  D       NEP    ++ P   E+Q V+       +    Y K TT
Sbjct:   65 WEG------NRGKNAEIPFRPGRVILQDFTGVPAIVDF-AAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAV-TDM-----KNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRI--NPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDAN-----NEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTT 618          

HSP 2 Score: 59.6918 bits (143), Expect = 2.403e-9
Identity = 62/205 (30.24%), Postives = 89/205 (43.41%), Query Frame = 0

Query:  510 PKDSEMQGVKLLIKAYGKCTTDHISMAG------PWLRYRGHLDNIS------------NNCL--------IGAVNAF--GKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFAN-EADYDL-IQEDDTFNFVDIADFAPNKQLTLEVVHADGS 684
            P+  +++    L+      T DHIS AG      P  RY     NIS            N+ +        I  VN F  GK      + + G+   V D A  YK      IV+   +YG GSSR+ AA  P  LGV AVI +S+ RIH +NL   G++ L ++  E+   L     + F+     +  P + L ++V   DGS
Sbjct:  657 PEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLAS-KNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGS 860          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 124.405 bits (311), Expect = 1.446e-29
Identity = 127/446 (28.48%), Postives = 194/446 (43.50%), Query Frame = 0

Query:  107 DLKRANEVS-----NEVFDFLGSVSNKY-GIGFWAPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR-----EAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTP-------DLSTNVGTEMTEKATKNDWPMK-----------------------VEWSLIGSCTNSSYEDLSR-ASSIAEFGQKNGLKLKS--NLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSF-------------NRNFAKRADGNPNTHA-FVASPELTAVIAFAGRLDFN 484
            D  R+N+ S      E F FL  V++ +  I    PG G++HQV LE  A          FP  + +GTD+HT    GLG++  GVG  DA  VM G    L FP+++G+R+ G +S ++   D++L + + L   G  GA VE+FGP    +S   + TI NM  E GA    F  DE   +YL  + R     E V      VK   ++D     N    F  +I+I+LS++ P ++GP          +++ +  + +  K     + +                        V  + I SCTN+S   +   A  +A+   + GL +       + PGS ++    +  G++     LG  I    C  C      Y+   N P +SIV +              NRNF  R D  P+  A ++ASP +    A AGR+D N
Sbjct:  159 DALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSV-VGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDE----NENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTC-----SYNSGPNLP-DSIVQAIKQGNLVCCGLLSGNRNFEGRLD--PDIRANYLASPLMVIAYAIAGRIDIN 591          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|1351858|sp|P49609.1|ACON_GRAGA (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 827.78 bits (2137), Expect = 0.000e+0
Identity = 413/732 (56.42%), Postives = 508/732 (69.40%), Query Frame = 0

Query:    8 IKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL-------------PPKD---------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            ++ VYA + +R++  R  + +PLTL+EK+LY HL D  P +   RG+ ++   P+R+A QDATAQMAL+QFM + + +VAVP+T+HCDHLI A+ GA++D+ +A   + EV+DFL S   KYG+GFW PG+GIIHQ+VLENYAFPG +MIGTDSHT NAGGLG  A+GVGGADAVDVM G+ WELK PK+IGV+LTGKL  W +PKDVILKVA ILT KGGTGAIVEYFG G  ++SCTG GTICNMGAEIGATTS F Y+  M  YL+AT R+ +A  A+   E L AD          +DQ+I+INLSEL P +NGPFTPDL+  + ++  E+  KN WP ++   LIGSCTNSSYED++R++S+ +    +G+K KS   I PGSE +R T  RDGIL  F   G T+  NACGPCIGQW R D PK  P NSI+ SFNRNF++R DGNP THAFVASPE+   ++ AG L FNP TD+L    G E K   P+G EL             PP D                            +   + +LIKA GKCTTDHISMAGPWL++RGHLDNISNN LIGAVN    + N VKN + GE+G VPDTARAYKA GVK +V+GD NYGEGSSREHAA+EPRHLG  AVIVKSFARIHETNLKKQGML LTF N ADYD I   D  + V + +  P + +T+ V  ADG+   I+LNHT+ND Q+
Sbjct:   40 LEPVYAAIDDRLNTVRSKLNRPLTLAEKVLYGHLDD--PERVPVRGETFLKLRPERVALQDATAQMALIQFMASARPQVAVPSTIHCDHLIAAEVGAEEDMAKAKSQNKEVYDFLASAGAKYGLGFWKPGSGIIHQIVLENYAFPGLLMIGTDSHTPNAGGLGACAVGVGGADAVDVMVGLPWELKAPKVIGVKLTGKLQEWASPKDVILKVAGILTVKGGTGAIVEYFGEGVDSLSCTGMGTICNMGAEIGATTSMFPYNSRMGDYLKATGRDGIASLADSFSEQLRADENA------VYDQLIEINLSELEPHINGPFTPDLAHPL-SKFKEEVEKNGWPAELTVGLIGSCTNSSYEDMARSASVVKQALSHGVKSKSIFNITPGSEQVRATISRDGILDTFTEAGGTVLANACGPCIGQWNRSDVPKGTP-NSIITSFNRNFSQRNDGNPQTHAFVASPEIVTAMSLAGSLKFNPATDSLQGADGAEFKLAAPSGDELPVMGFDPGEDTFQPPSDDSTSILVQIDPDSQRLSFLEPFPAWDGKDYTDMPVLIKARGKCTTDHISMAGPWLKFRGHLDNISNNMLIGAVNDENGEINNVKNAVTGEYGTVPDTARAYKAEGVKWVVIGDENYGEGSSREHAALEPRHLGGVAVIVKSFARIHETNLKKQGMLPLTFNNPADYDKIDSSDKVSLVGLKNLTPGEPVTMTVTKADGTSMDILLNHTFNDEQL 761          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|586946283|sp|C8VG90.1|ACON_EMENI (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Homocitrate dehydratase; Flags: Precursor)

HSP 1 Score: 822.387 bits (2123), Expect = 0.000e+0
Identity = 416/728 (57.14%), Postives = 505/728 (69.37%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTG--------------WELPPKD--------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y +M+E +D  R  + +PLT +EKILYSHL D   ++   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA PTTVHCDHLI+A+ G +KDL RANE++ EV+DFL S + KY IGFW PG+GIIHQ++LENYAFPGG+MIGTDSHT NAGGL + AIGVGGADAVDVMAG+ WELK PK+IGVRLTG++SGWTAPKD+ILKVA +LT KGGTGAI+EY GPG  ++S TG  TICNMGAEIGATTS F +++ M  YL+AT R+ + D A    + L  D          +DQ+I+INLSEL P +NGPFTPDL+T + ++  E    N WP +++  LIGSCTNSSYED+SRA+SIA+    +GLK KS   + PGSE IR T ERDG L   E  G  I  NACGPCIGQW R D  K  P NSIV S+NRNF  R D NP THAFV SP+L   ++ AG L+FNPLTDTL +  G+E K   PTG              ++ PP D                           +  G+ +LIK  GK TTDHISMAGPWL+YRGHLDNISNN LIGAVNA   + N +KN   GE+G VP TAR YKA GVK +V+GD NYGEGSSREHAA+EPRHLG  A+I +SFARIHETNLKKQGML LTF++ ADYD I  D T + +   + A +K +TL V   DG+   + L+HT+N++QI
Sbjct:   48 YKKMSENLDVVRRRLTRPLTYAEKILYSHL-DDPQNQDIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPTTVHCDHLIEAQLGGEKDLARANEINKEVYDFLASSTAKYNIGFWKPGSGIIHQIILENYAFPGGLMIGTDSHTPNAGGLAIAAIGVGGADAVDVMAGLPWELKAPKVIGVRLTGEMSGWTAPKDIILKVAGLLTVKGGTGAIIEYHGPGVNSLSATGMATICNMGAEIGATTSLFPFNDRMYDYLKATKRQQIGDFARSYAKDLREDEGA------EYDQLIEINLSELEPHINGPFTPDLATPI-SQFKEAVKANGWPEELKVGLIGSCTNSSYEDMSRAASIAQDALDHGLKAKSIFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDRKDVKKGTP-NSIVSSYNRNFTGRNDANPATHAFVTSPDLVVALSIAGTLNFNPLTDTLKDKDGKEFKLKAPTGDGLPSRGYDPGRDTYQAPPTDRSSVDVAVSPSSDRLQLLAGFQPWDGKDATGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGEANKIKNVFTGEYGAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFARIHETNLKKQGMLPLTFSDPADYDRIPPDATVDLL-CTELAVDKPMTLRVHPKDGASFDVKLSHTFNESQI 765          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74670431|sp|Q4WLN1.1|ACON_ASPFU (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Homocitrate dehydratase; Flags: Precursor)

HSP 1 Score: 821.617 bits (2121), Expect = 0.000e+0
Identity = 420/729 (57.61%), Postives = 498/729 (68.31%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTG--------------WELPPKD--------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKN-TIMLNHTYNDAQI 699
            Y +M+E +D  R  + +PLT +EK+LYSHL D    +   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA PTTVHCDHLI+A+ G  KDL RANE++ EV+DFL S + KY IGFW PG+GIIHQ+VLENYAFPGG+MIGTDSHT NAGGL M AIGVGGADAVDVMAG+ WELK PK+IGV+LTG++SGWT PKDVILKVA +LT KGGTGAI+EY GPG  ++SCTG GTICNMGAEIGATTS F +++ M  YL+AT R+ + D A +  + L  D          +DQ+I+INLSEL P +NGPFTPDL+T + ++  E    N WP +++  LIGSCTNSSYED+SRA+SIA     +GLK KS   + PGSE IR T ERDG L   E  G  I  NACGPCIGQW R D  K  P NSI+ S+NRNF  R D NP THAFVASP+L   +  AG L FNPLTD L +  G E     PTG              ++ PP D                           +  G+ +LIK  GK TTDHISMAGPWL+YRGHLDNISNN LIGAVNA   K N VKN+  GE+  VP TAR YKA GVK +V+GD NYGEGSSREHAA+EPRHLG  A+I +SFARIHETNLKKQGML LTFA+ ADYD I  +DT + +        K +TL V   DGS    I LNHT+N++QI
Sbjct:   50 YKKMSENLDIVRRRLQRPLTYAEKVLYSHL-DDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPTTVHCDHLIEAQVGGDKDLARANEINKEVYDFLASATAKYNIGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLAMAAIGVGGADAVDVMAGLPWELKAPKVIGVKLTGEMSGWTTPKDVILKVAGLLTVKGGTGAIIEYHGPGVTSLSCTGMGTICNMGAEIGATTSMFPFNDRMYDYLKATKRQHIGDFAREYAKELREDEGA------EYDQLIEINLSELEPHINGPFTPDLATPI-SKFKEAVETNKWPEELKVGLIGSCTNSSYEDMSRAASIARDALNHGLKAKSLFTVTPGSEQIRATIERDGQLQTLEEFGGVILANACGPCIGQWDRRDVKKGEP-NSIISSYNRNFTGRNDANPATHAFVASPDLVVAMTIAGTLKFNPLTDKLKDKDGNEFLLQPPTGEGLPAKGYDPGRDTYQAPPADRSSVNVAVSPTSDRLQLLAGFEPWDGKDANGIPILIKCQGKTTTDHISMAGPWLKYRGHLDNISNNMLIGAVNAENGKANSVKNKFTGEYDAVPATARDYKARGVKWVVIGDWNYGEGSSREHAALEPRHLGGLAIITRSFARIHETNLKKQGMLPLTFADPADYDKINPEDTVDLL-CTQLEVGKPMTLRVHPKDGSAPFDISLNHTFNESQI 768          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74856524|sp|Q54XS2.1|ACON_DICDI (RecName: Full=Probable aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 812.757 bits (2098), Expect = 0.000e+0
Identity = 412/729 (56.52%), Postives = 501/729 (68.72%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-----------------------DSEMQGVKL-----------------LIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHA-DGSKNTIMLNHTYNDAQI 699
            Y  +  ++ + R    KPLTL+EKI+Y HL D  PS    RG  Y+   PDR+A QDATAQMA+LQFM AG  + AVPTT+HCDHLI+A +G +KDL+ A +++ EV+DFL + + K+G+GFW PG+GIIHQ+VLENYAFPGG+MIGTDSHT NAGGLGMVA+GVGGADAVDVMAG+ WELK PK+IGV+LTG L GW++PKDVIL+VA+ILT KGGTGAIVEYFG G  ++SCTG  TICNMGAEIGATTS F +++ M  YL +T R  +A+AAN  K +L AD      P  ++DQ+I++NL  L P +NGPFTPDL   + ++  E    N+WP +++  LIGSCTNSSYED+SR++S+A+     G+  K+   I PGSE IR T ERDG +   E +G  +  NACGPCIGQW R D PK   KNSI+ S+NRNF  R D N NTHAFVASPE+   +  AG + FNP+TD LT+  G + K   PTG ELP +                       DSE   ++L                 LIK  GKCTTDHISMAGPWL+YRGHLDNISNN LIGA+N+   K N V NQ  GE G VP  AR YK  GV  IVVGD NYGEGSSREHAA+EPRHLG  A++VKSFARIHETNLKKQG+L LTFAN +DYD I  DD  + + + D AP KQLTL V  A  GS+  I  NHT N  QI
Sbjct:   33 YEGIDAKLKQFRLHHNKPLTLAEKIIYGHLED--PSTKVERGITYLKLHPDRVAMQDATAQMAVLQFMSAGLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLSTSAKKFGMGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLGMVAVGVGGADAVDVMAGIPWELKAPKIIGVKLTGSLKGWSSPKDVILRVADILTVKGGTGAIVEYFGSGVESLSCTGMATICNMGAEIGATTSLFPFNKRMVDYLNSTGRSNIANAANSFKHNLVAD------PNAHYDQLIELNLDTLEPYINGPFTPDLGHPL-SKFAESVKTNNWPAELKVGLIGSCTNSSYEDMSRSASVAQQALDKGITAKAKFTITPGSEQIRATIERDGQMKVLEKVGGVVLANACGPCIGQWKREDVPK-GEKNSIITSYNRNFTGRNDSNVNTHAFVASPEIVTALTIAGDITFNPMTDFLTDKDGNKFKLTPPTGDELPSRGFDAGENTYQPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPTVARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGILPLTFANPSDYDKISGDDRISIIGLKDLAPGKQLTLIVKSAKQGSEFEIKANHTMNAGQI 751          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|21264394|sp|P34455.2|ACON_CAEEL (RecName: Full=Probable aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 804.668 bits (2077), Expect = 0.000e+0
Identity = 412/725 (56.83%), Postives = 513/725 (70.76%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y ++++ V   ++ + +PLTLSEKILY HL D   ++   RG  Y+   PDR+A QDATAQMA+LQF+ +G  K AVP+T+HCDHLI+A++G  +DL RA +++ EVF+FL +  +KYG+GFW PG+GIIHQ++LENYAFPG ++IGTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IG++LTGKL+GWT+ KDVILKVA+ILT KGGTGAIVEYFGPG  ++S TG GTICNMGAEIGATTS F Y+ESM +YL AT R+ +A+ A K K+ LTAD    AN    +DQ+I+INL  L P +NGPFTPDL++++  ++ E A KN WP+ V+ SLIGSCTNSSYED++RA+SIA+     GLK K+   I PGSE +R T ERDG+   F + G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP TH FV SP++T  +A +GRLDFNPLTD LT   G + K   PTG +LPPK                                       +++ +K+LIK  GKCTTDHIS AGPWL+YRGHLDNISNN  + A+NA   + N VKNQ+ GE+G VP TAR YKA GV+ + +GD NYGEGSSREHAA+EPRHLG  A+IVKSFARIHETNLKKQGML LTFAN ADYD I   D  + V ++ FAP K LT      +GSK  + LNHT+N+ QI
Sbjct:   42 YEKLSQTVKIVKDRLKRPLTLSEKILYGHL-DQPKTQDIERGVSYLRLRPDRVAMQDATAQMAMLQFISSGLPKTAVPSTIHCDHLIEAQKGGAQDLARAKDLNKEVFNFLATAGSKYGVGFWKPGSGIIHQIILENYAFPGLLLIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGIKLTGKLNGWTSAKDVILKVADILTVKGGTGAIVEYFGPGVDSISATGMGTICNMGAEIGATTSVFPYNESMYKYLEATGRKEIAEEARKYKDLLTADDG--AN----YDQIIEINLDTLTPHVNGPFTPDLASSI-DKLGENAKKNGWPLDVKVSLIGSCTNSSYEDMTRAASIAKQALDKGLKAKTIFTITPGSEQVRATIERDGLSKIFADFGGMVLANACGPCIGQWDR-QDVKKGEKNTIVTSYNRNFTGRNDANPATHGFVTSPDITTAMAISGRLDFNPLTDELTAADGSKFKLQAPTGLDLPPKGYDPGEDTFQAPSGSGQVDVSPSSDRLQLLSPFDKWDGKDLEDMKILIKVTGKCTTDHISAAGPWLKYRGHLDNISNNLFLTAINADNGEMNKVKNQVTGEYGAVPATARKYKADGVRWVAIGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGMLPLTFANPADYDKIDPSDNVSIVGLSSFAPGKPLTAIFKKTNGSKVEVTLNHTFNEQQI 757          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|384872308|sp|O13966.2|ACON_SCHPO (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 803.127 bits (2073), Expect = 0.000e+0
Identity = 408/728 (56.04%), Postives = 502/728 (68.96%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK---------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKN-TIMLNHTYNDAQI 699
            Y ++ + ++  ++ + +PLT SEKILY HL D + ++   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VAVP TVHCDHLI+A +G   DL+RAN  + EV+DFL +   KY IGFW PG+GIIHQ+VLENYAFPGG++IGTDSHT NAGGLGMVAIGVGGADAVDVMA + WELK PK+IGV+LTG+L GWT+PKDVILKVA ILT KGGTGAIVEYFGPG  ++SCTG GTICNMGAEIGATTS F ++  M  YLRAT+R A+AD A +    + AD         ++DQ+I+I+L+ L P LNGPFTPDL+T + ++  E   KNDWP +++  LIGSCTNSSYED+SRA+SI +     G+K KS   I PGSE +R T  RDG L      G  +  NACGPCIGQW R  D K   KNSIV S+NRNF  R D NP THAFV SP++   + F+G ++FNPLTDTL +  G + KF  PTG  LP K                                         +M+G+K+LIKA GKCTTDHIS AGPWL+YRGHL NISNN +IGA+NA   + N +K+QL GE+  VP+ A  Y+  G++ + +G+ N+GEGSSREHAA+EPR+LG AAVI KSFARIHETNLKKQG+L LTFA+ A YD I   DT +   +  FAP K LTL V  ADGS   +  LNHT+N  QI
Sbjct:   52 YQRIKDNLEIVKKRLNRPLTYSEKILYGHLDDPV-NQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNKEVYDFLQTACAKYNIGFWRPGSGIIHQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVKLTGQLKGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPRMSEYLRATNRSAIADYAEEFAPIIAADENA------HYDQIIEIDLNTLEPHLNGPFTPDLATPI-SKFKEAVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQQAIDKGIKTKSLFTITPGSEQVRATLTRDGQLDTMRKAGGIVLANACGPCIGQWKR-TDVKKGEKNSIVTSYNRNFTGRNDANPATHAFVTSPDIVTAMVFSGDMNFNPLTDTLKDKDGNDFKFEPPTGAGLPSKGYDPGSNTYVAPSSVNVKDVAIDPHSKRLQRLTPFKKWDGKDMKGLKILIKAKGKCTTDHISAAGPWLKYRGHLQNISNNYMIGAINAENGEANKLKDQLTGEYKTVPNVAIDYRDHGIRWVTLGEQNFGEGSSREHAALEPRYLGGAAVITKSFARIHETNLKKQGLLPLTFADPAAYDKISPFDTVDIDGLTTFAPGKPLTLVVHPADGSAEWSTKLNHTFNKDQI 770          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|961512531|sp|D4AT77.1|ACON_ARTBC (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 803.127 bits (2073), Expect = 0.000e+0
Identity = 408/728 (56.04%), Postives = 495/728 (67.99%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTG--------------WELPPKD--------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y +MAE +D  R  + +PLT +EKILYSHL D    +   RGK Y+   PDR+ACQDATAQMA+LQFM AG   VA P TVHCDHLI+A+ G  KDL RANE++ EV+DFL +   KY IGFW PG+GIIHQ++LENY FPGG+MIGTDSHT N GGLGM AIGVGGADAVDVMAG+ WELK P +IGV+LTG++SGWTAPKD+ILKVA ILT KGGTGAI+EY G G  ++SCTG GTICNMGAEIGATTS F +++ M  YL+AT R+++ D +    E L  D          +DQ+I+INLSEL P +NGPFTPDL+T + ++  E   +N+WP +++  LIGSCTNSSYED+SRA+SIA     +G+K KS   + PGSE IR T ERDG L   E  G  I  NACGPCIGQW R D  KN   NSI+ S+NRNF  R D NP THAFV SP+L   +  AG L+FNPLTD L +  G E     PTG              ++ PP D                           + +G+ +LIK  GK TTDHISMAGPWL+YRGHLDNISNN LIGAVNA   + N VKN   GE+  VP TARAYKA G+  +V+GD NYGEGSSREHAA++PRHLG  A+I +SFARIHETNLKKQGML LTFA+ ADYD I      + +   + A  K +TL V   DG+   + L+HT+N++QI
Sbjct:   41 YKKMAENLDIVRARLNRPLTFAEKILYSHL-DDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIEAQLGGDKDLARANEINKEVYDFLSTSCAKYNIGFWKPGSGIIHQILLENYCFPGGLMIGTDSHTPNGGGLGMAAIGVGGADAVDVMAGLPWELKAPNVIGVKLTGQMSGWTAPKDIILKVAGILTVKGGTGAIIEYHGDGVNSLSCTGMGTICNMGAEIGATTSVFPFNDRMYDYLKATKRQSIGDFSRVYAEGLRPDENA------QYDQLIEINLSELEPHINGPFTPDLATPI-SKFKEAVKENNWPSELKVGLIGSCTNSSYEDMSRAASIARDALNHGIKAKSLFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDRKDVKKNE-ANSIISSYNRNFTGRNDANPATHAFVTSPDLVVALTIAGTLNFNPLTDKLKDKDGNEFMLAPPTGEGLPANGYDPGRDTYQAPPADRASISVAVSPSSDRLQILEPFKAWDGKDAKGIPILIKCEGKTTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAENGERNSVKNFETGEYDSVPATARAYKARGIPWVVIGDWNYGEGSSREHAALQPRHLGGMAIITRSFARIHETNLKKQGMLPLTFADPADYDRIPPTAMVDLM-CTELAVGKPMTLRVHPKDGASFDVKLSHTFNESQI 758          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|1168308|sp|P19414.2|ACON_YEAST (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 793.882 bits (2049), Expect = 0.000e+0
Identity = 405/728 (55.63%), Postives = 493/728 (67.72%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTG--------------WELPPKDSEMQGVK--------------------------LLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y Q  E +D  R+ + +P T +EKILY HL D    +   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VA P TVHCDHLIQA+ G +KDLKRA +++ EV+DFL S + KY +GFW PG+GIIHQ+VLENYAFPG ++IGTDSHT NAGGLG +AIGVGGADAVDVMAG  WELK PK++GV+LTGK++GWT+PKD+ILK+A I T KGGTG IVEYFG G    S TG GTICNMGAEIGATTS F +++SM  YL AT R  +AD A    + L +     A+ +  +D+V++I+L+ L P +NGPFTPDL+T V ++M E A  N+WP+ V   LIGSCTNSSYED+SR++SI +    +GLK K+   + PGSE IR T ERDG L  F+  G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R DGNP THAFVASPEL    A AG L FNPLTD L +  G E     P G              ++ PP D     VK                          +LIKA GK TTDHISMAGPWL+YRGHL+NISNN +IGA+NA  KK N VKN   GE+ GVPDTAR Y+  G+K +V+GD N+GEGSSREHAA+EPR LG  A+I KSFARIHETNLKKQG+L L F N ADYD I  DD  + + +A+ AP K +T+ V   +G     +L HT+ND QI
Sbjct:   41 YKQNVETLDIVRKRLNRPFTYAEKILYGHL-DDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMGFWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMAGRPWELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGMGTICNMGAEIGATTSVFPFNKSMIEYLEATGRGKIADFAKLYHKDLLS-----ADKDAEYDEVVEIDLNTLEPYINGPFTPDLATPV-SKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSRSASIVKDAAAHGLKSKTIFTVTPGSEQIRATIERDGQLETFKEFGGIVLANACGPCIGQWDRR-DIKKGDKNTIVSSYNRNFTSRNDGNPQTHAFVASPELVTAFAIAGDLRFNPLTDKLKDKDGNEFMLKPPHGDGLPQRGYDAGENTYQAPPADRSTVEVKVSPTSDRLQLLKPFKPWDGKDAKDMPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENKKANCVKNVYTGEYKGVPDTARDYRDQGIKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINPDDRIDILGLAELAPGKPVTMRVHPKNGKPWDAVLTHTFNDEQI 760          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|113159|sp|P16276.1|ACON_PIG (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0
Identity = 402/728 (55.22%), Postives = 503/728 (69.09%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL--------------PPKDS--------------------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y  + + +D  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G +KDL+RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG LSGWT+PKDVILKVA ILT KGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R  +A+ A++ K+HL  D      P  ++DQVI+INLSEL P +NGPFTPDL+  V  E+   A K  WP+ +   LIGSCTNSSYED+ R++++A+    +GLK KS   I PGSE IR T ERDG      ++G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPE+   +A AG L FNP TD LT   G++ K   P   EL              PPKDS                          +++ +++LIK  GKCTTDHIS AGPWL++RGHLDNISNN LIGA+N   +K N V+N +  EFG VPDTAR YK  G++ +V+GD NYGEGSSREH A+EPRHLG  A+I KSFARIHETNLKKQG+L LTFA+ ADY+ I   D      + DFAP K L   + H +G++ TI+LNHT+N+ QI
Sbjct:   45 YDLLEKNIDIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPD------PGCHYDQVIEINLSELKPHINGPFTPDLAHPVA-EVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENRKANSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGSSREHRALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFAPGKPLKCIIKHPNGTQETILLNHTFNETQI 763          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|6686275|sp|Q99798.2|ACON_HUMAN (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 788.104 bits (2034), Expect = 0.000e+0
Identity = 401/728 (55.08%), Postives = 504/728 (69.23%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL--------------PPKDS--------------------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y  + + ++  R+ + +PLTLSEKI+Y HL D   S+   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G +KDL+RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG LSGW++PKDVILKVA ILT KGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T RE +A+ A++ K+HL  D      P  ++DQ+I+INLSEL P +NGPFTPDL+  V  E+ + A K  WP+ +   LIGSCTNSSYED+ R++++A+    +GLK KS   I PGSE IR T ERDG      +LG  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPE+   +A AG L FNP TD LT   G++ +   P   EL              PPKDS                          +++ +++LIK  GKCTTDHIS AGPWL++RGHLDNISNN LIGA+N    K N V+N +  EFG VPDTAR YK  G++ +V+GD NYGEGSSREHAA+EPRHLG  A+I KSFARIHETNLKKQG+L LTFA+ ADY+ I   D      + DF P K L   + H +G++ TI+LNHT+N+ QI
Sbjct:   45 YDLLEKNINIVRKRLNRPLTLSEKIVYGHL-DDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPD------PGCHYDQLIEINLSELKPHINGPFTPDLAHPVA-EVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDYLTGTDGKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCIIKHPNGTQETILLNHTFNETQI 763          

BLAST of aconitate hydratase vs. nr
Match: gi|1334593528|ref|WP_103070321.1| (aconitate hydratase [Aquimarina sp. w01])

HSP 1 Score: 1262.67 bits (3266), Expect = 0.000e+0
Identity = 609/738 (82.52%), Postives = 647/738 (87.67%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPKD---------------------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVY+Q+A+RV+ AREV GKPLTL+EKILYSHLWD    +AF RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA KDLK AN++S+EVF+FL SVS+KYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGM+AIGVGGADAVDVMAGMAWELKFPKLIGV+LTGKLSGWTAPKDVILKVAEILT KGGTGAIVEYFGPGA +MSCTGKGTICNMGAEIGATTSTFGYDESMERYLRAT+R  VADAANKVKEHLTAD EVY NPEQYFDQVI+INLSELGPLLNGPFTPDLST VG  MTEKA  NDWP++VEW LIGSCTNSSYEDLSRASSIA+     GLK+K+ LGINPGSE +R+T +RDGIL  FE L A IFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTA IA AGRLDFNPLTD L N+ GEEV F+EPTGWELPPK                                        SE++G KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQL GEFGGVPDTARAYKAAG+ TIVVGDHNYGEGSSREHAAMEPRHLGV AVIVKSFARIHETNLKKQGMLGLTFANE +YDLIQEDDTFNF D+  FAPNKQLTLE+VHADGSK+TIMLNHTYN++QI
Sbjct:    1 MAFDIDMIKKVYSQLADRVNSAREVTGKPLTLAEKILYSHLWDAKAKEAFIRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKEGAAKDLKNANDISSEVFNFLESVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMIAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGATSMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATERADVADAANKVKEHLTADPEVYENPEQYFDQVIEINLSELGPLLNGPFTPDLSTAVGGTMTEKAKANDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRYTADRDGILGIFEQLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAAIAVAGRLDFNPLTDKLINENGEEVMFDEPTGWELPPKGFEVKDNGYLAPVEDGSGVEVKVSPTSERLQLLTPFEPIGSEVKGAKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEFGGVPDTARAYKAAGIPTIVVGDHNYGEGSSREHAAMEPRHLGVCAVIVKSFARIHETNLKKQGMLGLTFANEKEYDLIQEDDTFNFTDLDQFAPNKQLTLEIVHADGSKDTIMLNHTYNESQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|1124659721|ref|WP_074939182.1| (aconitate hydratase [Algibacter lectus] >gi|1098024981|emb|SFD46406.1| aconitase [Algibacter lectus])

HSP 1 Score: 1262.67 bits (3266), Expect = 0.000e+0
Identity = 610/738 (82.66%), Postives = 649/738 (87.94%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-------------------------DSE--------------MQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIK+VY +MA+RVDKAR +VGKPLTLSEKILYSHLWDG P+KAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK GA  DLK AN VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGKLSGWTAPKDVILKVA ILTAKGGTGAIVEYFGPGA +MSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VADAAN VKEHLT DAEVYANPEQYFD++I+INLSEL PLLNGPFTPDLST VG ++ EKAT N+WP+KVEW LIGSCTNSSYEDLSRASSIA+     GLK+KS LGINPGSE +R+T +RDGIL  FENL A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNP+TD L N+ GEEV F+EPTGWELPPK                         DSE              + G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFG+KTNFVKNQ+ G++GGVPDTARAYKAAG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANE+DYDLIQEDDTFNF D+  FAP+KQLTLEVVHADGSK+ I LNHTYNDAQI
Sbjct:    1 MAFDIDMIKEVYTKMAQRVDKARSIVGKPLTLSEKILYSHLWDGNPTKAFSRGKDYVDFAPDRIACQDATAQMALLQFMQAGKTKVAVPTTVHCDHLIQAKDGAATDLKHANSVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWTAPKDVILKVAGILTAKGGTGAIVEYFGPGATSMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRSDVADAANAVKEHLTGDAEVYANPEQYFDELIEINLSELSPLLNGPFTPDLSTPVGKDINEKATANEWPLKVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKSELGINPGSEQVRYTADRDGILGIFENLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVKDNGYLAPEADGSHVQVKVAEDSERLQLLTPFTPLGNDISGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGQKTNFVKNQITGDYGGVPDTARAYKAAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANESDYDLIQEDDTFNFTDLNAFAPDKQLTLEVVHADGSKDVIKLNHTYNDAQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|943615453|ref|WP_055445346.1| (aconitate hydratase [Lacinutrix himadriensis])

HSP 1 Score: 1262.67 bits (3266), Expect = 0.000e+0
Identity = 610/738 (82.66%), Postives = 647/738 (87.67%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK---------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILY+HLWDG P+KAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGK +VAVPTTVHCDHLIQAK GA  DLK AN VS+EVFDFL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGKLSGWTAPKDVILKVA I++AKGGTGAIVEYFGPGA +MSCTGKGTICNMGAEIGATTSTFGYD+SMERYLRATDR  VAD ANK++E+LT DAEVYANPEQYFDQVI+INLSELGPLLNGPFTPDLST VG +M E+A  NDWP+KVEW LIGSCTNSSYEDLSRASSIA+     GLK+KS LGINPGSE +R+T  RDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNPLTD+L N++G+EV+F+EPTGWELPPK                                        + + G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNA+GKKTNFVKNQL G+FGGVPDTARAYKAAG+KTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANE DYDLIQEDDTFNF D+  FAP KQLTLEVVHADGSK+ IML+HTYN  QI
Sbjct:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYNHLWDGSPTKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPQVAVPTTVHCDHLIQAKDGATSDLKHANSVSSEVFDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWTAPKDVILKVAGIVSAKGGTGAIVEYFGPGATSMSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADVADEANKIREYLTGDAEVYANPEQYFDQVIEINLSELGPLLNGPFTPDLSTPVGKKMGEQAKANDWPLKVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKSELGINPGSEQVRYTAARDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINEEGQEVRFDEPTGWELPPKGFAVEDAGFVEPVADGSKVEVSVSPTSERLQLLTPFEPLGNTINGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAYGKKTNFVKNQLTGDFGGVPDTARAYKAAGIKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFTDLNAFAPGKQLTLEVVHADGSKDIIMLDHTYNQPQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|738197156|ref|WP_036153088.1| (aconitate hydratase [Maribacter forsetii])

HSP 1 Score: 1262.67 bits (3266), Expect = 0.000e+0
Identity = 607/739 (82.14%), Postives = 646/739 (87.42%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPP----------------------------------------KDSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA M+ERVDKAREVVGKPLTLSEKILYSHLWDG P++AFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK GA  DLK AN  S EVFDFL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG +SGWTAPKDVILKVAEILT KGGTGAIVEYFG GA  +SCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  +ADAAN VKEHLTAD+EVYANPEQYFD+VI+INLS+L PLLNGPFTPDLST VG++MTEKAT  DWP+ VEW LIGSCTNSSYEDLSRASSIA+     G+K+KS LGINPGSE +R+T +RDGIL  FE L A IFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA +GRLDFNP+TD L N KGEEV F+EPTGWELPP                                        KD  + G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFGKKTN VKNQL+GEFGGVPDTARAYKAAG+++IVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANE+DYDLIQEDDTFNF+DIA+FAP+KQLT+EVVHADGSK+ I +NHTYN AQI
Sbjct:    1 MAFDIDMIKKVYASMSERVDKAREVVGKPLTLSEKILYSHLWDGSPTEAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKNGATADLKSANTTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGNISGWTAPKDVILKVAEILTVKGGTGAIVEYFGEGAKNLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADIADAANTVKEHLTADSEVYANPEQYFDEVIEINLSKLTPLLNGPFTPDLSTKVGSDMTEKATSQDWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGIKMKSELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPMTDKLINQKGEEVMFDEPTGWELPPKGFAVEDAGYLAPNEDGSGVEVKVASDSERLQLLEPFTPIKDESLMGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNMVKNQLNGEFGGVPDTARAYKAAGIRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANESDYDLIQEDDTFNFIDIAEFAPDKQLTVEVVHADGSKDVIKVNHTYNAAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|1224444513|ref|WP_092467267.1| (aconitate hydratase [Winogradskyella thalassocola] >gi|1086704116|emb|SDH36193.1| aconitase [Winogradskyella thalassocola])

HSP 1 Score: 1262.28 bits (3265), Expect = 0.000e+0
Identity = 613/738 (83.06%), Postives = 643/738 (87.13%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-------------------------DS--------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA M ERVD AR +VGKPLTLSEKILYSHLWDG P+KAFTRGKDYVDFAPDR+ACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK GA  DLK AN+ S+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVA+ILT KGGTGAIVEYFGPGA +MSCTGKGTICNMGAEIGATTSTFGYD+SMERYLRATDR  VADAAN ++ +LTAD EVYANPEQYFDQVI+INLSELGPLLNGPFTPDLST VG +MTEKA  NDWPM+VEW LIGSCTNSSYEDLSRASSIA+     GLK+KS LGINPGSE +RFT ERDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNPLTD L N+ GEEV F EPTGWELPPK                         DS              E+ G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNA+GKKTNFVKNQL GEFGGVPDTARAYKAAG+KTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NEADYDLIQEDDTFNF D+ +FAP+KQL +EVVHADGSK+ I LNHTYN AQI
Sbjct:    1 MAFDIDMIKKVYANMTERVDAARGIVGKPLTLSEKILYSHLWDGKPNKAFTRGKDYVDFAPDRVACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKDGAATDLKHANQTSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAQILTVKGGTGAIVEYFGPGATSMSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADVADAANAIRPYLTADEEVYANPEQYFDQVIEINLSELGPLLNGPFTPDLSTEVGRDMTEKAKANDWPMQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKSELGINPGSEQVRFTAERDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDKLINENGEEVMFEEPTGWELPPKGFEVKENGYLAPVADGSGVVVSVDPDSERIQLLTPFEPIGNEINGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAYGKKTNFVKNQLTGEFGGVPDTARAYKAAGIKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFDNEADYDLIQEDDTFNFTDLNEFAPDKQLHMEVVHADGSKDVIALNHTYNQAQI 738