BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 285/502 (56.77%), Postives = 367/502 (73.11%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPE--QYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK 511
            Y ++ ++++  ++ + +PLTLSEK+LYSHL D   ++   RG+ Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLIQA+   KKDL RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYAFPG +M+GTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IGV+LTGKLSGWT+PKDVILKVA ILT KGGTGAIVEYFG G  ++SCTG GTICNMGAEIGATTS F Y+  M+ YL +T+R  +A+ A+K +       EV  +P+    +DQV++I+L+ L P +NGPFTPDL+T +G E+ EKA KN WP++V+  LIGSCTNSSYED++R ++I +     G+K KSN  I PGSE IR T ERDG+       G  +  NACGPCIGQW R +  K   KN+IV S+NRNF  R D NP TH FV SPE+T  ++ AG LDF+P +D +    G E+K N P G ELP +
Sbjct:  628 YEKLVQKLEIVKDRLNRPLTLSEKVLYSHL-DDPKNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIQAQIEGKKDLARAKDLNAEVYNFLSTSGAKYGVGFWKPGSGIIHQIILENYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRSDIANLADKYR-------EVLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPDLATPIG-ELGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAIIKQALAKGMKSKSNFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWDRQNITK-GEKNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQ 1119          

HSP 2 Score: 53.1434 bits (126), Expect = 2.566e-7
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 0

Query:  648 EADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            +ADYD I+ DD     D+ +FAP + +   + H+DGS   I LNH++N+ QI
Sbjct: 1171 KADYDKIRPDDLITLNDLVNFAPGRPVVATIKHSDGSSENISLNHSFNEQQI 1222          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 131.339 bits (329), Expect = 9.226e-32
Identity = 164/575 (28.52%), Postives = 239/575 (41.57%), Query Frame = 0

Query:   42 WDGMPSKAFTRGKDY-VDFAPDRIACQDATAQMALLQFMQAGKDKV------------AVPTTVHCDHLIQAKQGAKKDLKRANE-----VSNEVFDFLG-SVSNKYGIGFWAPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANP--EQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDW----------------------------PMKVEWSL---------IGSCTNSSYEDLSR-ASSIAEFGQKNGLKLKSNL--GINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKN------SIVHSFNRNFAKRADGNPNTHA-FVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGW-ELPPKDSEMQGVK-------LLIKAYGKCTT 530
            W+G       RGK+  + F P R+  QD T   A++ F  A +D V              PT +  DH IQ     +      NE      + E F FL    S+   +    PG+GI+HQV LE  A          FP  + +GTDSHT    GLG+V  GVGG +A  VM G    +  P+++G R++G         D++L + + L   G  G  VE++GPG   +S   + TI NM  E GAT   F  D     YLR T+RE+      +   HLT     Y +   +  F Q++ ++L  +    +GP  P     V T+M     KND+                            P   E+ L         I SCTN+S   +   A  +A+     GL +   +   ++PGS ++ +  +  G++   + LG +I       CIG     +D      N      + V S NRNF  R   NP+T A ++ASP L    A AGR+D +   + L  D       NEP    ++ P   E+Q V+       +    Y K TT
Sbjct:   65 WEG------NRGKNAEIPFRPGRVILQDFTGVPAIVDF-AAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAV-TDM-----KNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRI--NPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDAN-----NEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTT 618          

HSP 2 Score: 59.6918 bits (143), Expect = 2.403e-9
Identity = 62/205 (30.24%), Postives = 89/205 (43.41%), Query Frame = 0

Query:  510 PKDSEMQGVKLLIKAYGKCTTDHISMAG------PWLRYRGHLDNIS------------NNCL--------IGAVNAF--GKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFAN-EADYDL-IQEDDTFNFVDIADFAPNKQLTLEVVHADGS 684
            P+  +++    L+      T DHIS AG      P  RY     NIS            N+ +        I  VN F  GK      + + G+   V D A  YK      IV+   +YG GSSR+ AA  P  LGV AVI +S+ RIH +NL   G++ L ++  E+   L     + F+     +  P + L ++V   DGS
Sbjct:  657 PEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLAS-KNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGS 860          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 124.405 bits (311), Expect = 1.446e-29
Identity = 127/446 (28.48%), Postives = 194/446 (43.50%), Query Frame = 0

Query:  107 DLKRANEVS-----NEVFDFLGSVSNKY-GIGFWAPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR-----EAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTP-------DLSTNVGTEMTEKATKNDWPMK-----------------------VEWSLIGSCTNSSYEDLSR-ASSIAEFGQKNGLKLKS--NLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSF-------------NRNFAKRADGNPNTHA-FVASPELTAVIAFAGRLDFN 484
            D  R+N+ S      E F FL  V++ +  I    PG G++HQV LE  A          FP  + +GTD+HT    GLG++  GVG  DA  VM G    L FP+++G+R+ G +S ++   D++L + + L   G  GA VE+FGP    +S   + TI NM  E GA    F  DE   +YL  + R     E V      VK   ++D     N    F  +I+I+LS++ P ++GP          +++ +  + +  K     + +                        V  + I SCTN+S   +   A  +A+   + GL +       + PGS ++    +  G++     LG  I    C  C      Y+   N P +SIV +              NRNF  R D  P+  A ++ASP +    A AGR+D N
Sbjct:  159 DALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSV-VGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDE----NENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTC-----SYNSGPNLP-DSIVQAIKQGNLVCCGLLSGNRNFEGRLD--PDIRANYLASPLMVIAYAIAGRIDIN 591          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|110282935|sp|P20004.4|ACON_BOVIN (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 784.637 bits (2025), Expect = 0.000e+0
Identity = 400/728 (54.95%), Postives = 504/728 (69.23%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL--------------PPKDS--------------------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y  + + ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G +KDL+RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG LSGWT+PKDVILKVA ILT KGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R  +A+ A++ K+HL  D+        ++DQ+I+INLSEL P +NGPFTPDL+  V  E+   A K  WP+ +   LIGSCTNSSYED+ R++++A+    +GL+ KS   I PGSE IR T ERDG      ++G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPE+   +A AG L FNP TD LT   G++ K   P   EL              PPKDS                          +++ +++LIK  GKCTTDHIS AGPWL++RGHLDNISNN LIGA+N    K N V+N +  EFG VPDTAR YK  G++ +V+GD NYGEGSSREHAA+EPRHLG  A+I KSFARIHETNLKKQG+L LTFA+ ADY+ I   D      + DFAP K LT  + H +G++ TI+LNHT+N+ QI
Sbjct:   45 YDLLEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGC------HYDQLIEINLSELKPHINGPFTPDLAHPVA-EVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQQVDVSPTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCIIKHPNGTQETILLNHTFNETQI 763          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|224471814|sp|P82611.2|ACON_CANAL (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 783.867 bits (2023), Expect = 0.000e+0
Identity = 400/730 (54.79%), Postives = 494/730 (67.67%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPP------------------------------------------KDSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y +  E V+  +  + +PLT +EK+LY HL D   ++   RG  Y+   PDR+ACQDATAQMA+LQFM AG  +VA P+TVHCDHLIQA+ G  KDL RA +++ EV+DFL +   KY +GFW PG+GIIHQ+VLENYAFPG ++IGTDSHT NAGGLG +AIGVGGADAVDVM+G+ WELK PK+IGV+LTGK+SGWT+PKD+ILK+A I T KGGTG+IVEYFG G    SCTG GTICNMGAEIGATTS F +++SM  YL AT R  +A  A   K+   +     A+    +DQVI+I+L+ L P +NGPFTPDL+T V ++M E A  N WP++V+  LIGSCTNSSYED++RA+SI +    +GLK K+   ++PGSE +R T  RDG L  FE+ G  +  NACGPCIGQW R  D K   KN+IV SFNRNF  R DGNP THAFVASPE+  V A +G L FNP+TDTL    G+E K  EP G  LPP                                          KD+E   + +LIKA GK TTDHISMAGPWL+YRGHL+NISNN +IGA+NA   K N V+N   G++ GVP TA AY+ AG K +V+GD N+GEGSSREHAA+EPR LG  A+I KSFARIHETNLKKQG+L L F N ADYD I  DD  + + +   AP K + L V   +G     +L HT+N  Q+
Sbjct:   42 YKKHLENVEIVKSRLNRPLTYAEKLLYGHL-DDPHNQEIERGVSYLKLRPDRVACQDATAQMAILQFMSAGIPQVATPSTVHCDHLIQAQVGGPKDLARAIDLNKEVYDFLSTACAKYNLGFWKPGSGIIHQIVLENYAFPGALLIGTDSHTPNAGGLGQLAIGVGGADAVDVMSGLPWELKAPKIIGVKLTGKMSGWTSPKDIILKLAGITTVKGGTGSIVEYFGSGVDTFSCTGMGTICNMGAEIGATTSVFPFNDSMVDYLNATGRSEIAQFAQVYKKDFLS-----ADEGAEYDQVIEIDLNTLEPHINGPFTPDLATPV-SKMKETAIANGWPLEVKVGLIGSCTNSSYEDMTRAASIIKDAGAHGLKSKALYTVSPGSEQVRATIARDGQLKTFEDFGGVVMANACGPCIGQWDR-QDIKKGDKNTIVSSFNRNFTARNDGNPATHAFVASPEMATVYAISGDLGFNPITDTLVGADGKEFKLKEPQGVGLPPDGYDPGENTYQAPPEDRASVEVVISPTSDRLQKLSPFKPWDGKDAER--LPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENGKANEVRNHYTGKYDGVPQTAAAYRDAGHKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINFDDEVDLIGLTTLAPGKDVILRVHPKEGEAWEAVLTHTFNSEQL 761          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391212|sp|Q99KI0.1|ACON_MOUSE (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 781.171 bits (2016), Expect = 0.000e+0
Identity = 399/728 (54.81%), Postives = 501/728 (68.82%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL--------------PPKDS--------------------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y  + + ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G +KDL+RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG LSGWT+PKDVILKVA ILT KGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R  +A+ A + K+HL  D      P   +DQVI+INL+EL P +NGPFTPDL+  V  ++   A K  WP+ +   LIGSCTNSSYED+ R++++A+    +GLK KS   I PGSE IR T ERDG      ++G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPE+   +A AG L FNP TD LT   G++ K   P   EL              PPKDS                          +++ +++LIK  GKCTTDHIS AGPWL++RGHLDNISNN LIGA+N    K N V+N +  EFG VPDTAR YK  G++ +V+GD NYGEGSSREHAA+EPRHLG  A+I KSFARIHETNLKKQG+L LTFA+ +DY+ I   D      + DFAP K L   + H +G++ TI+LNHT+N+ QI
Sbjct:   45 YDLLEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRTDIANLAEEFKDHLVPD------PGCQYDQVIEINLNELKPHINGPFTPDLAHPVA-DVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLKCVIKHPNGTQETILLNHTFNETQI 763          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391194|sp|Q9ER34.2|ACON_RAT (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 399/728 (54.81%), Postives = 501/728 (68.82%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWEL--------------PPKDS--------------------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y  + + ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G +KDL+RA +++ EV++FL +   KYG+GFW PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG LSGWT+PKDVILKVA ILT KGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R  +A+ A + K+HL  D      P   +DQVI+INL+EL P +NGPFTPDL+  V  ++   A K  WP+ +   LIGSCTNSSYED+ R++++A+    +GLK KS   I PGSE IR T ERDG      ++G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPE+   +A AG L FNP TD LT   G++ K   P   EL              PPKDS                          +++ +++LIK  GKCTTDHIS AGPWL++RGHLDNISNN LIGA+N    K N V+N +  EFG VPDTAR YK  G++ +V+GD NYGEGSSREHAA+EPRHLG  A+I KSFARIHETNLKKQG+L LTFA+ +DY+ I   D      + DFAP K L   + H +G++ TI+LNHT+N+ QI
Sbjct:   45 YDLLEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGTLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLAEEFKDHLVPD------PGCQYDQVIEINLNELKPHINGPFTPDLAHPVA-DVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNCIIKHPNGTQETILLNHTFNETQI 763          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|395398603|sp|Q9P7D4.3|ACON2_SCHPO (RecName: Full=Homocitrate dehydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 694.886 bits (1792), Expect = 0.000e+0
Identity = 368/741 (49.66%), Postives = 464/741 (62.62%), Query Frame = 0

Query:   12 YAQMAERVDKAREVV-GKPLTLSEKILYSHLWDGMPSKAFT-------RGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELP----------------------------PKDS--------------EMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDD---TFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            Y ++  ++ + R+ + G+ LTL+EK+LYSHL +  P ++F+       RG  Y+   PDR+A QDA+AQMALLQFM  G +K  +P ++HCDHLI   +GA  D+  +   + E+FDFL S + KYGI FW PG+GIIHQ+VLENYA PGGMM+GTDSHT NAGGLGM+AIGVGGADAVD M    WELK PK+IGV LTG +SGWT PKD+IL +A  LT +GGTG I+EYFGPG  ++SCTG  T+CNMGAE+GATTS F Y +SM RYL AT R  VADAA++V       A   A+    +DQ+IDINLSEL P LNGPFTPDLST V ++  E   KN WP K+   LIGSCTNSSY+D++    + E     GLK K    + PGSE IR T ERDGI    E  GAT+  NACGPCIG W R DD  +   N+I+ SFNRNF  R DGNP+T  F+ SP + A   F+  L F+P  DTL    G+  KF  P G ELP                            PK                EM+ +K+ +K  GKCTTDHIS AG WL+Y+GHL NI NN LIGA+NA   + N   +  +G+   +P+    +K  G   +VV +HNYGEGS+REHAA++PR +    ++ KSFARIHETNLKKQG+L LTF NEADY+ I  +D   T     + +   ++ +TL V   DGS   I   HT +  QI
Sbjct:   44 YEKLMGKLQQVRKFLPGQKLTLAEKVLYSHLVN--PEESFSGVSPSDIRGSLYLKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNKEIFDFLQSAAKKYGIQFWGPGSGIIHQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVNLTGAMSGWTTPKDLILHLAGKLTVRGGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYLIATHRAEVADAASEVHSEYNYLA---ADTGAKYDQIIDINLSELTPSLNGPFTPDLSTPV-SKFGEAIEKNKWPKKLSAGLIGSCTNSSYQDMTCVVDVVEQAISAGLKPKVPFLVTPGSEQIRATIERDGITERLEEAGATVLANACGPCIGMWKRTDDIASGEPNAILTSFNRNFRSRNDGNPSTMNFLTSPVIVAAKIFSSDLAFDPTHDTLQTPDGKAFKFRPPQGVELPSAGFIAGDSSYIPEPNPQPVPETEVTIDPKSDRLEALEPFEPYQGGEMENLKVAVKVKGKCTTDHISAAGKWLKYKGHLSNICNNTLIGAMNAATGEVNRAYD--NGKGMTIPELMWKWKKDGQPWLVVAEHNYGEGSAREHAALQPRAMNGRIILTKSFARIHETNLKKQGVLPLTFVNEADYEKIDAEDKVSTRGIEQLLEGVLDQPITLVVTKKDGSVVEIPCKHTMSKDQI 776          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|728783|sp|P39533.1|ACON2_YEAST (RecName: Full=Homocitrate dehydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 684.871 bits (1766), Expect = 0.000e+0
Identity = 368/743 (49.53%), Postives = 475/743 (63.93%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVGK-PLTLSEKILYSHLWDGMPSKAFT-------RGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPK-NSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELP----------------------------PKDSEMQGVK-------------LLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDD---TFNFVDI--ADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            YA++   +DK +++    PLTL+EKILYSHL D  P ++ T       RG  Y+   PDR+A QDA+AQMALLQFM  G ++ +VP ++HCDHLI  K G  KDL  +   + EVFDFL S + +YGI FW PG+GIIHQ+VLEN++ PG MM+GTDSHT NAGGLG +AIGVGGADAVD + G  WELK PK++GV+LTGKL+GW+ PKDVI K+A +LT +GGTG IVEYFG G   +SCTG  TICNMGAEIGATTSTF Y E+ +RYL+AT+R  VA+AA      L     + A+ +  +D+VI+I+LS + P +NGPFTPDLST + ++  EK+ K +WP KV   LIGSCTNSSY+D+SR   + +   K GLK +    + PGSE IR T ERDGI+  F+  GA +  NACGPCIGQW R D  K + + N+I  SFNRNF  R DGN NT  F+ SPE+   ++++G   FNPLTD++    G++ KF  P G ELP                            P    +Q ++             +L+K  GKCTTDHIS AG WL+Y+GHL+NIS N LIGA N    + N   + LDG    +P     +K+ G    V+ +HNYGEGS+REHAA+ PR LG   ++VKSFARIHETNLKKQG+L LTFANE+DYD I   D   T N VD+   D     ++ +++   +G   TI   HT +  QI
Sbjct:   35 YAKLLTNLDKIKQITNNAPLTLAEKILYSHLCD--PEESITSSDLSTIRGNKYLKLNPDRVAMQDASAQMALLQFMTTGLNQTSVPASIHCDHLIVGKDGETKDLPSSIATNQEVFDFLESCAKRYGIQFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKILGVKLTGKLNGWSTPKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTGMATICNMGAEIGATTSTFPYQEAHKRYLQATNRAEVAEAA---DVALNKFNFLRADKDAQYDKVIEIDLSAIEPHVNGPFTPDLSTPI-SQYAEKSLKENWPQKVSAGLIGSCTNSSYQDMSRVVDLVKQASKAGLKPRIPFFVTPGSEQIRATLERDGIIDIFQENGAKVLANACGPCIGQWNREDVSKTSKETNTIFTSFNRNFRARNDGNRNTMNFLTSPEIVTAMSYSGDAQFNPLTDSIKLPNGKDFKFQPPKGDELPKRGFEHGRDKFYPEMDPKPDSNVEIKVDPNSDRLQLLEPFKPWNGKELKTNVLLKVEGKCTTDHISAAGVWLKYKGHLENISYNTLIGAQNKETGEVNKAYD-LDGTEYDIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRFLGGEILLVKSFARIHETNLKKQGVLPLTFANESDYDKISSGDVLETLNLVDMIAKDGNNGGEIDVKITKPNGESFTIKAKHTMSKDQI 770          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74669593|sp|Q4WJ90.1|ACON2_ASPFU (RecName: Full=Putative aconitate hydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0
Identity = 356/741 (48.04%), Postives = 457/741 (61.67%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVG--KPLTLSEKILYSHLWDGMPSKAFT--------RGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPP-----------------------------------------KDSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLI---QEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQ 698
            Y ++   +++ R+V+G  + LTL+EKILY+HL D       T        RGK  +   PDR+A QDA+AQMALLQFM  G    AVP ++HCDH+I  ++GA  DL  + E + EVFDFL S + +YGI FW PGAGIIHQ VLENYA PG MM+GTDSHT NAGGLG +AIGVGGADAVD +    WELK P+++GVRL G+LSGW +PKD+IL +A  LT +GGTG ++EY GPG   +SCTG  T CNMGAE+GATTS F +  SM  YL+AT R  VA AA ++      +  + A+    +DQ+I I+LS L P +NGPFTPDLS  + ++      +N WP  +   LIGSCTNSSYED++RA  + +     GLK K++  I PGSE IR T +RD  LA F   G T+  NACGPCIGQW R D       N+I  S+NRNF  R DGN  T  F+ASPE+   +A++G   FNP+TDTL    GEE KF  P G +LP                                           + E+ G+K+L K  G+CTTD IS AGPWL+Y+GHL NIS N LIGAVNA   +TN   +   G    +PD A  +KA G++ +VV + NYGEGS+REHAA++PR+LG   ++ KSFARIHETNLKKQG++ LTFA++ADYD I    + DT    +         + L+V    G K TI +NHT +  Q
Sbjct:   44 YPKIVRNLEQVRKVLGSSRALTLAEKILYAHL-DNAEESLLTGTNNGKDIRGKANLKLKPDRVAMQDASAQMALLQFMSCGLPSTAVPASIHCDHMIVGERGADTDLPASIEGNREVFDFLESAAKRYGIEFWPPGAGIIHQSVLENYAAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALVDAPWELKAPRILGVRLEGRLSGWASPKDIILHLAGKLTVRGGTGYVIEYHGPGVETLSCTGMATCCNMGAEVGATTSVFPFSPSMVPYLQATHRGHVAQAAAEIAASGPKNL-LRADDGAEYDQLITIDLSTLEPHVNGPFTPDLSVRL-SDFANTVRENKWPETLGAGLIGSCTNSSYEDMTRAEDLVKQASAAGLKPKADFFITPGSEQIRATLDRDQTLASFSEAGGTVLANACGPCIGQWKRTDGVAKGEDNAIFTSYNRNFPGRNDGNRRTMNFLASPEIVTALAYSGSTTFNPMTDTLKTPSGEEFKFRPPQGSDLPSAGFADGNPALQPSAGVPDASVEVIVSPTSERLALLEPFAPFPEGELSGLKVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDDA-GNKHSIPDLAARWKADGIEWLVVAEDNYGEGSAREHAALQPRYLGGRIIVAKSFARIHETNLKKQGVVPLTFADKADYDRIDACDQVDTVGLYETLQSGGQGSIQLQVTKKSGEKLTIPVNHTLSKDQ 780          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74596317|sp|Q5B6D6.1|ACON2_EMENI (RecName: Full=Putative aconitate hydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 354/741 (47.77%), Postives = 463/741 (62.48%), Query Frame = 0

Query:   12 YAQMAERVDKAREVVG--KPLTLSEKILYSHLWDGMPSKAFT--------RGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-----------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPN---KQLTLEVVHADGSKNTIMLNHTYNDAQ 698
            Y+++ + +++ R+V+G  + LTL+EKILYSHL D       T        RGK  +   PDR+A QDA+AQMALLQFM  G    AVP ++HCDH+I  ++GA  DL  + + + EVFDFL S S +YGI FW PGAGIIHQ VLENY+ PG MM+GTDSHT NAGGLG +AIGVGGADAVD +    WELK P+++GVRL GKL GW APKD+IL +A  LT +GGTG ++EY GPG   +S TG  TICNMGAE+GATTS F +  +   YL+AT+R  VA+AA K+     + + + A+    +D++I I+LS L P +NGPFTPDLS  + +   E   KN+WP      LIGSCTNSSYED++RA  + +     GLK K++L I PGSE IR T +RD  L+ F + G T+  NACGPCIGQW R DD      N+I  S+NRNF  R DGN  T  F+ASPEL   + +AG   FNP+TD++T   G E +F  PTG +LP K                                         +S++Q + +L K  G+CTTD IS AGPWL+Y+GHL NIS N LIGAVNA   +TN   ++  G+   +PD A  +KA G + +VV + NYGEGS+REHAA++PR+LG   ++ KSFARIHETNLKKQG++ LTFA++ADYD I   D      + +   N    ++ L V    G +  I + HT +  Q
Sbjct:   44 YSKIVQNLEQVRKVLGSSRALTLAEKILYSHL-DNAEESLLTGTNNGRDIRGKADLKLKPDRVAMQDASAQMALLQFMSCGLPSTAVPASIHCDHMIVGERGADTDLPASIQGNKEVFDFLESASKRYGIEFWPPGAGIIHQSVLENYSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALVDAPWELKAPRILGVRLEGKLQGWAAPKDIILHLAGKLTVRGGTGFVIEYHGPGVETLSTTGMATICNMGAEVGATTSLFPFSPNHVPYLKATNRADVAEAAAKIASA-GSSSLLRADTSAEYDELITIDLSTLEPHINGPFTPDLSVPL-SRFAETVRKNNWPETFNAGLIGSCTNSSYEDMTRAEHLVKQANAAGLKPKADLFITPGSEQIRATLDRDQTLSTFSSAGGTVLANACGPCIGQWKRTDDVPKGTDNAIFTSYNRNFPGRNDGNRRTMNFLASPELVTALTYAGSTTFNPVTDSITTPSGSEFRFEPPTGQDLPSKGFEAGNPAFQPSAPVPDSSVEVKVSPTSTRLALLEPFAPFPNSDLQNLSVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDEA-GKQHTIPDLAAQWKAQGREWLVVAEENYGEGSAREHAALQPRYLGGRVILAKSFARIHETNLKKQGVVPLTFADKADYDRIDACDVVATEGLYETLKNGGKGEVKLRVTKKSGEEIVIPVKHTLSADQ 780          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|122426093|sp|Q1RKD5.1|ACNA_RICBR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 191.045 bits (484), Expect = 6.295e-50
Identity = 207/743 (27.86%), Postives = 307/743 (41.32%), Query Frame = 0

Query:   57 VDFAPDRIACQDATAQMALLQF--MQAGKDKVA---------VPTTVHCDHLIQAKQ---GAKKDLKRANEVSN--EVFDFLGSVSNKYG-IGFWAPGAGIIHQVVLE----------NYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRAT--DREAVADAANKVKEHLTADAEVYANPE---QYFDQVIDINLSELGPLLNGP---------------FTPDLST----NVGTEMTEKATKNDWPM---KVEWSLIGSCTNSSYED-LSRASSIAEFGQKNGLKLKS--NLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDP------KNAPKNSIVHSFNRNFAKRADGNPNTHA-FVASP----------------------------------------ELTAVIA---------------FAGRLDFNPLTDTLTN----DKGEEVKFNEPTGWELPPKDS--EMQGVKLLIKAYGKCTTDHISMAG------PWLRY-----------------RGHLDNISN----NCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFAN 647
            +DF P R+  QD T   A++    M+    K+          +P  +  DH +       G+  D   A E+    E + FL      +       PG GI HQV LE            A+P  + +GTDSHT    GL ++  GVGG +A   M G    +  P++IGV+LTGKL+G     D++LK+ E+L  K   G  VE++G G  AM+   + TI NM  E GAT   F  D+   +YL  T  D+E +     K+ E      +++ N +   +Y D +++++LSE+   L GP               F  +LST    N+  +        ++ +    V  + I SCTN+S    +  A+ +A+   + GLK+K      + PGS+++    +  G+    + LG  +    C  CIG     +        KN    + V S NRNF  R   NP T A ++ASP                                        E+  VIA               F+G  ++  L  T ++    DK      N P    +  K+S  +++  ++L       TTDHIS AG      P  +Y                 RG+ + +      N  I      G +  F  NQL      + D A  YKA GV  ++     YG GSSR+ AA  P+ LGV AVI +SF RIH +NL   G+L L F N
Sbjct:   75 IDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYASGSSFDKNVAMEMRRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVWHSNGVAYPDSL-VGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGTATATDLVLKITEMLRKKKVVGKFVEFYGEGLKAMTIADRATISNMAPEYGATCGFFPIDQETIKYLELTGRDKEQI-----KLVEEYAKAQDLWCNFDDAAEYTD-ILELDLSEVTSSLAGPRRPQDRVNLGDVSSGFKKELSTFSSNNISIDTKHAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKAIEQGLKVKPWVKTSLAPGSKVVTEYLKSSGLNQYLDQLGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRI--NPLTKASYLASPILVVAYALSGSLNIDLTNHPLGKNDKGRDVYLKDIWPSKEEIDKVIANSINSSMFVEKYSDIFSGTKEWQSLEVTSSSNYAWDKSSTYINNPPYFENIGSKNSIKDIKSARILAIFGDSITTDHISPAGSISKTSPAAKYLTDHQISPIDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLKNMQQTIYDAAMDYKANGVSAVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGVLPLIFTN 808          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|20137396|sp|Q92G90.1|ACNA_RICCN (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 186.037 bits (471), Expect = 3.480e-48
Identity = 210/760 (27.63%), Postives = 313/760 (41.18%), Query Frame = 0

Query:   57 VDFAPDRIACQDATAQMALLQF--MQAGKDKVA---------VPTTVHCDHLIQ-----AKQGAKKDLKRANEVSNEVFDFLGSVSNKYG-IGFWAPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQY--FDQVIDINLSELGPLLNGPFTP-------DLSTNVGTEMTEKATKN-DWPMK--------------VEWSLIGSCTNSSYED-LSRASSIAEFGQKNGLKLKS--NLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDP------KNAPKNSIVHSFNRNFAKRADGNPNTHA-FVASP-----------------------------------ELTAVIA---------------FAGRLDFNPL----TDTLTNDKGEEVKFNEPTGWELPPKDS--EMQGVKLLIKAYGKCTTDHISMAG------PWLRY-----------------RGHLDNISN----NCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFA-NEADYDLIQEDDTFNFVDIADFAPN 671
            +DF P R+  QD T   A++    M+    K+          +P  +  DH +      AK    K+++   + + E + FL      +       PG GI HQV LE  A          +P  + +GTDSHT    GL ++  GVGG +A   M G    +  P++IGV+LTGKL+G     D++L V E+L  K   G  VE+FG G   +    + TI NM  E GAT   F  D+   +YL  T RE       ++ E    +  ++ + E    + +V++++LS +   L GP  P       D+++N   E+   A  N D   K              V  + I SCTN+S    +  A+ +A+   ++GLK+K      + PGS+++    +  G+    + LG  +    C  CIG     +        KN    + V S NRNF  R   NP T A ++ SP                                   E+  VIA               F+G  ++  L    + T   +K      N P   ++  K++  +++  K+L       TTDHIS AG      P  +Y                 RG+ + +      N  I      G +  F  NQL+G    + D A  YKA  V  ++     YG GSSR+ AA  P  LGV AVI +SF RIH +NL   G+L LTF  N    DL  + D    +DI   + N
Sbjct:   76 IDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYAAKDSFNKNVQMEMKRNIERYAFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVWHKDGTLYPDSL-VGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGIATATDLVLTVTEMLRKKKVVGKFVEFFGEGLKNLMIADRATISNMSPEYGATCGFFPIDQETIKYLELTGREKTQI---RLVEQYATEQNLWYDFEHAVEYTEVLELDLSMVHGSLAGPKRPQDRVNLNDVASNFKYELPNFALDNKDIDKKYAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLKVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDELGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRI--NPLTKASYLGSPILVVAYALSGTLNIDLTNMPIGENIYLKDLWPSKEEIDEVIANSINSSMFIEKYSDIFSGTKEWKDLQVTNSSTYNWNKNSTYINNPPYFKDIGSKNNIQDIKSAKILAILGDSITTDHISPAGSISKTSPAAKYLTDHHIEPLDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLNGTQQTIYDAAMDYKAHDVSVVIFAGKEYGSGSSRDWAAKGPGLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDL--KLDGSETIDITGLSEN 827          

BLAST of aconitate hydratase vs. nr
Match: gi|149354607|gb|EDM43171.1| (aconitate hydratase [unidentified eubacterium SCB49])

HSP 1 Score: 1299.26 bits (3361), Expect = 0.000e+0
Identity = 629/739 (85.12%), Postives = 661/739 (89.45%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPP----------------------------------------KDSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYAQMAERVD AREVVGKPLTLSEKILY+HLWDG+ +KAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGK  VAVPTTVHCDHLIQAKQGAK DLK ANEVSNEVFDFL SVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VADAANKVK++LTAD EVYA+P+ YFDQVI+INLSELGPLLNGPFTPDLST VG +MTEKA  NDWP+KVEWSLIGSCTNSSYEDLSRASSIA+FG KNGLKLK++LGINPGSE +RFTTERDGIL  FE+LGA IFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTA IAFAGRLDFNP+TDTL NDKGEE+KF EPTGWELPP                                        KD+++  VKLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFGKKTNFVKNQL G+F GVPDTAR YKA GV +IVVGDHNYGEGSSREHAAMEPRHLGVAAV+VKSFARIHETNLKKQGMLGLTFANEADYD IQEDDTFNFVDIA FAP+K LT+EV HADGS +TI+ NH+YN AQI
Sbjct:    1 MAFDIDMIKKVYAQMAERVDAAREVVGKPLTLSEKILYNHLWDGVATKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKSVAVPTTVHCDHLIQAKQGAKADLKHANEVSNEVFDFLESVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGATAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANKVKDYLTADPEVYADPDSYFDQVIEINLSELGPLLNGPFTPDLSTQVGADMTEKANANDWPLKVEWSLIGSCTNSSYEDLSRASSIADFGLKNGLKLKADLGINPGSETVRFTTERDGILKSFEDLGAKIFTNACGPCIGQWARYEDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAAIAFAGRLDFNPMTDTLINDKGEEIKFAEPTGWELPPKGFEVEENGYVAPMEDGSGVVVSVSPESERLQLLTPFEPIKDADLSNVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGDFAGVPDTARDYKANGVYSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANEADYDKIQEDDTFNFVDIASFAPDKPLTIEVTHADGSTDTIVANHSYNAAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|503517474|ref|WP_013751915.1| (aconitate hydratase [Dokdonia sp. 4H-3-7-5] >gi|332171153|gb|AEE20408.1| aconitate hydratase [Dokdonia sp. 4H-3-7-5])

HSP 1 Score: 1299.26 bits (3361), Expect = 0.000e+0
Identity = 627/739 (84.84%), Postives = 661/739 (89.45%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK----------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA M ERVD AR++VGKPLTLSEKILYSHLW+G  SKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA+KDLK AN+VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VADAAN VKE+LTAD EVYANPEQYFDQVIDINLSELGPLLNGPFTPDLST VG +MTEKATKN+WP+ VEW LIGSCTNSSYEDLSRASSIA+     G+K+KS LGINPGSEL+R+TTERDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA +GRLDFNP+TDTL N+ G+EV F+EPTGWELPPK                                        DSEMQG+KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQ+DGEFGGVPDTARAYK AG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNF+DIA FAP+K LT+E+VHADGSK+TI +NHTYNDAQI
Sbjct:    1 MAFDIDMIKKVYANMTERVDAARDLVGKPLTLSEKILYSHLWEGKASKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAEKDLKNANDVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGATAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANDVKEYLTADKEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTTVGKDMTEKATKNEWPLNVEWGLIGSCTNSSYEDLSRASSIAQQALDKGIKMKSELGINPGSELVRYTTERDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEVTAAIAISGRLDFNPMTDTLLNEDGQEVMFDEPTGWELPPKGFEVKDNGYLAPVEDGSGVIVKVAADSERLELLTPFEPILDSEMQGMKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQMDGEFGGVPDTARAYKKAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFIDIASFAPDKPLTVELVHADGSKDTIEVNHTYNDAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|737354433|ref|WP_035336638.1| (aconitate hydratase [Dokdonia sp. PRO95])

HSP 1 Score: 1291.95 bits (3342), Expect = 0.000e+0
Identity = 624/739 (84.44%), Postives = 658/739 (89.04%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPP----------------------------------------KDSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA M+ERVD AR++VGKPLTLSEKILYSHLW+G  SKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA+KDLK AN+VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VADAAN VKE+LTAD EVYANPEQYFDQVIDINLSELGPLLNGPFTPDLST VG +MTEKATKN+WP+ VEW LIGSCTNSSYEDLSRASSIA+     G+K+KS+LGINPGSEL+R+TTERDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA +GRLDFNPLTDTL N+ G+EV F+EPTGWELPP                                        KDSEMQG+KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQL GE+GGVPDT R YK AG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NEADYDLIQEDDTFNFVDIA FAP+  LT+E+VHADGSK+TI +NHTYNDAQI
Sbjct:    1 MAFDIDMIKKVYANMSERVDAARDLVGKPLTLSEKILYSHLWEGKASKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAEKDLKNANDVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGATAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANDVKEYLTADKEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTTVGKDMTEKATKNEWPLNVEWGLIGSCTNSSYEDLSRASSIAQQALDKGIKMKSDLGINPGSELVRYTTERDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPLTDTLLNEDGQEVMFDEPTGWELPPKGFDVQDNGYLAPVEDGSGVEVKVASDSERLELLTPFEPIKDSEMQGMKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEYGGVPDTQREYKKAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFQNEADYDLIQEDDTFNFVDIASFAPDTPLTVEIVHADGSKDTIKVNHTYNDAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|737342365|ref|WP_035324653.1| (aconitate hydratase [Dokdonia donghaensis] >gi|700263650|gb|KGO05604.1| aconitate hydratase [Dokdonia donghaensis DSW-1] >gi|1033646438|gb|ANH61191.1| Aconitate hydratase precursor [Dokdonia donghaensis DSW-1])

HSP 1 Score: 1284.24 bits (3322), Expect = 0.000e+0
Identity = 617/739 (83.49%), Postives = 657/739 (88.90%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK----------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA MAERVD AR++VGKPLTLSEKILYSHLWDG PS AFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA  DLK AN VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG +SGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA ++SCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDR  VADAAN VKE+LTADA+VYANPEQYFDQVI+INL+EL PLLNGPFTPDLST VG++MTEKATKN+WP++VEW LIGSCTNSSYEDLSRASSIA+     G+K+K+ LGINPGSEL+R+TTERDGIL  FENL A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNP+TDTL N+ G+EVKF+EPTGWELPPK                                        DSEMQG+KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQL GE+GGVPDT R YK AG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NEADYDLIQEDDTFNF+DIADFAP+  LT+E+VHADGSK+TI +NHTYNDAQI
Sbjct:    1 MAFDIDMIKKVYASMAERVDAARDLVGKPLTLSEKILYSHLWDGKPSTAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAATDLKNANNVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAESLSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANNVKEYLTADADVYANPEQYFDQVIEINLTELNPLLNGPFTPDLSTAVGSDMTEKATKNEWPLEVEWGLIGSCTNSSYEDLSRASSIAQQALDKGIKMKAELGINPGSELVRYTTERDGILDIFENLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLLNEDGQEVKFDEPTGWELPPKGFEVQDNGYLAPVEDGSGVVVSVSPDSERLELLTPFTPIQDSEMQGMKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEYGGVPDTQREYKKAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFQNEADYDLIQEDDTFNFIDIADFAPDTPLTVEIVHADGSKDTIKVNHTYNDAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|545721499|ref|WP_021779283.1| (aconitate hydratase [Dokdonia sp. MED134] >gi|85819231|gb|EAQ40390.1| aconitate hydratase [Dokdonia sp. MED134])

HSP 1 Score: 1283.47 bits (3320), Expect = 0.000e+0
Identity = 616/739 (83.36%), Postives = 657/739 (88.90%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK----------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA MAERVD AR++VGKPLTLSEKILYSHLWDG PS AFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA  DLK AN VS+EVF+FL SVSNKYGIGFW PGAGIIHQVV+ENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG +SGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA ++SCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDR  VADAAN +KE+LTADA+VYANPEQYFDQVI+INL+EL PLLNGPFTPDLST VG++MTEKATKN+WP++VEW LIGSCTNSSYEDLSRASSIA+     G+K+K+ LGINPGSEL+RFTTERDGIL  FENL A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNP+TDTL N+ G+EVKF+EPTGWELPPK                                        DSEMQG+KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQL GE+GGVPDT R YK AG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NEADYDLIQEDDTFNF+DIADFAP+  LT+E+VHADGSK+TI +NHTYNDAQI
Sbjct:    1 MAFDIDMIKKVYASMAERVDAARDLVGKPLTLSEKILYSHLWDGKPSTAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAATDLKNANNVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVIENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAESLSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANNIKEYLTADADVYANPEQYFDQVIEINLTELNPLLNGPFTPDLSTAVGSDMTEKATKNEWPLEVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGIKMKAELGINPGSELVRFTTERDGILDIFENLEAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLLNEDGQEVKFDEPTGWELPPKGFEVQDNGYLAPVEDGSNVVVSVSPDSERLELLTPFTPIQDSEMQGMKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEYGGVPDTQREYKKAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFQNEADYDLIQEDDTFNFIDIADFAPDTPLTVEIVHADGSKDTIKVNHTYNDAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|1055903411|gb|AOE08105.1| (aconitate hydratase [uncultured bacterium])

HSP 1 Score: 1282.32 bits (3317), Expect = 0.000e+0
Identity = 615/739 (83.22%), Postives = 657/739 (88.90%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK----------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYA MAERVD AR++VGKPLTLSEKILYSHLWDG PS AFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA  DLK AN VS+EVF+FL SVSNKYGIGFW PGAGIIHQVV+ENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG +SGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA ++SCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDR  VADAAN +KE+LTADA+VYANPEQYFDQVI+INL+EL PLLNGPFTPDLST VG++MTEKATKN+WP++VEW LIGSCTNSSYEDLSRASSIA+     G+K+K+ LGINPGSEL+R+TTERDGIL  FENL A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNP+TDTL N+ G+EVKF+EPTGWELPPK                                        DSEMQG+KLLIKA+GKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQL GE+GGVPDT R YK AG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NEADYDLIQEDDTFNF+DIADFAP+  LT+E+VHADGSK+TI +NHTYNDAQI
Sbjct:    1 MAFDIDMIKKVYASMAERVDAARDLVGKPLTLSEKILYSHLWDGKPSTAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAATDLKNANNVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVIENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAESLSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANNIKEYLTADADVYANPEQYFDQVIEINLTELNPLLNGPFTPDLSTAVGSDMTEKATKNEWPLEVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGIKMKAELGINPGSELVRYTTERDGILDIFENLEAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLLNEDGQEVKFDEPTGWELPPKGFEVQDNGYLAPVEDGSNVVVSVSPDSERLELLTPFTPIQDSEMQGMKLLIKAFGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEYGGVPDTQREYKKAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFQNEADYDLIQEDDTFNFIDIADFAPDTPLTVEIVHADGSKDTIKVNHTYNDAQI 739          

BLAST of aconitate hydratase vs. nr
Match: gi|1140880807|ref|WP_076733509.1| (aconitate hydratase [Lacinutrix venerupis] >gi|1134985423|gb|APY00603.1| aconitate hydratase [Lacinutrix venerupis])

HSP 1 Score: 1281.16 bits (3314), Expect = 0.000e+0
Identity = 619/738 (83.88%), Postives = 655/738 (88.75%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPKD---------------------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVY+QMAERVD AREVVGKPLTLSEKILY+HLWDG  +KAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA  DLK AN+VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG+LSGWTAPKDVILKVAEILT KGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRE VADAANKVKE+LTADAEVYANPEQYFDQVI+INLSEL PLLNGPFTPDLST+VG +MTEKA  NDWP++VEW LIGSCTNSSYEDLSRASSIA+     GLK+K+ LGINPGSE +R+T +RDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTA IA AGRLDFNP+TD L N+ G+EV F+EP GWELPPK                                        +E++G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFGKKTNFVKNQL GEF GVPDTARAYKAAG+K++VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANE DYDLIQEDDTFNFVDIA F+P+KQLTLEVVHADGSK+TIMLNHTYN AQI
Sbjct:    1 MAFDIDMIKKVYSQMAERVDAAREVVGKPLTLSEKILYNHLWDGKATKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAATDLKHANDVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGELSGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGATAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREDVADAANKVKEYLTADAEVYANPEQYFDQVIEINLSELNPLLNGPFTPDLSTSVGNDMTEKAKANDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEKVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAAIAIAGRLDFNPMTDKLINEDGQEVMFDEPRGWELPPKGFEVKDNGYLAPVEDGSGVEVVVSPTSERLQLLTPFEPIGNEIKGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEFAGVPDTARAYKAAGIKSVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFVDIASFSPDKQLTLEVVHADGSKDTIMLNHTYNQAQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|1119374375|ref|WP_072350825.1| (aconitate hydratase [Flavobacteriaceae bacterium A100])

HSP 1 Score: 1276.92 bits (3303), Expect = 0.000e+0
Identity = 618/738 (83.74%), Postives = 653/738 (88.48%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK---------------------------------------DSEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVY+QMAERVD AR+VVG+PLTLSEKILY+HLWDG  +KAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA KDLK AN VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VAD ANKVKE+LTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLST VG EMTEKAT N+WP++VEW LIGSCTNSSYEDLSRASSIA+     GLK+K+ LGINPGSE +R+T ERDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTA IA +GRLDFNP+TD L N++G+EV F+EPTGWELPPK                                        ++++G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNN LIGAVNAFG KTNFVKNQL GE+GGVPDTARAYKAAGV T+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVD+ +FAP+KQLTLEVVHADGSK+ I LNHTYN AQI
Sbjct:    1 MAFDIDMIKKVYSQMAERVDAARDVVGRPLTLSEKILYNHLWDGKATKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKEGAVKDLKHANSVSSEVFEFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGATAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADEANKVKEYLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTTVGKEMTEKATANEWPLQVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKAELGINPGSEQVRYTAERDGILGIFEQLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAAIAISGRLDFNPMTDKLINEEGQEVMFDEPTGWELPPKGFAVEDAGYVEPVVDGSKVEVSVSPTSERLQLLTPFKPLGNKIEGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNMLIGAVNAFGMKTNFVKNQLTGEYGGVPDTARAYKAAGVHTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDLNEFAPDKQLTLEVVHADGSKDVIKLNHTYNQAQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|1101425494|gb|OIQ22668.1| (aconitate hydratase [Lacinutrix sp. MedPE-SW])

HSP 1 Score: 1276.92 bits (3303), Expect = 0.000e+0
Identity = 617/738 (83.60%), Postives = 655/738 (88.75%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPKD---------------------------------------SEMQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVYAQMAERVD AREVVGKPLTLSEKILY+HLWDG  +KAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK+GA  DLK AN+VS+EVF+FL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG+LSGWTAPKDVILKVAEILT KGGTGAIVEYFGPGA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRE VADAANKVKE+LTADAEVYANPEQYFDQVI+INLSEL PLLNGPFTPDLST+VG++MTEKA  NDWP++VEW LIGSCTNSSYEDLSRASSIA+     GLK+K++LGINPGSE +R+T +RDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTA IA AGRLDFNP+TD L N+ G+EV F+EP GWELPPK                                        +E+ G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFGKKTNFVKNQL GE+ GVPDTARAYKAAG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNF+DIA+FAPNKQLTLEVVHADGSK+ I LNHTYN +QI
Sbjct:    1 MAFDIDMIKKVYAQMAERVDAAREVVGKPLTLSEKILYNHLWDGKATKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKEGAAADLKHANDVSSEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGELSGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREDVADAANKVKEYLTADAEVYANPEQYFDQVIEINLSELNPLLNGPFTPDLSTSVGSDMTEKAKANDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKADLGINPGSEKVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAAIAIAGRLDFNPMTDKLINEDGQEVMFDEPRGWELPPKGFEVKDNGYLAPVEDGSGVEVVVSPTSERLQLLTPFEPIGNEINGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNFVKNQLTGEYAGVPDTARAYKAAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFIDIAEFAPNKQLTLEVVHADGSKDVIKLNHTYNQSQI 738          

BLAST of aconitate hydratase vs. nr
Match: gi|654830135|ref|WP_028282892.1| (MULTISPECIES: aconitate hydratase [Flavobacteriaceae] >gi|1273378388|gb|PIB30076.1| aconitate hydratase [Gaetbulibacter sp. 5U11])

HSP 1 Score: 1275.38 bits (3299), Expect = 0.000e+0
Identity = 619/738 (83.88%), Postives = 654/738 (88.62%), Query Frame = 0

Query:    1 MAFDIDMIKKVYAQMAERVDKAREVVGKPLTLSEKILYSHLWDGMPSKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKQGAKKDLKRANEVSNEVFDFLGSVSNKYGIGFWAPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAEILTAKGGTGAIVEYFGPGAIAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDREAVADAANKVKEHLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTNVGTEMTEKATKNDWPMKVEWSLIGSCTNSSYEDLSRASSIAEFGQKNGLKLKSNLGINPGSELIRFTTERDGILAPFENLGATIFTNACGPCIGQWARYDDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPELTAVIAFAGRLDFNPLTDTLTNDKGEEVKFNEPTGWELPPK-------------------------DSE--------------MQGVKLLIKAYGKCTTDHISMAGPWLRYRGHLDNISNNCLIGAVNAFGKKTNFVKNQLDGEFGGVPDTARAYKAAGVKTIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFVDIADFAPNKQLTLEVVHADGSKNTIMLNHTYNDAQI 699
            MAFDIDMIKKVY+QM ERVD AR+VVGKPLTLSEKILYSHLWDG P+ AFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAK GA  DLK AN VS+EVFDFL SVSNKYGIGFW PGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVA I++AKGGTGAIVEYFG GA AMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDR  VADAAN+VKE+LTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLST VG  MTEKAT+N+WP++VEW LIGSCTNSSYEDLSRASSIA+     G+K+K+ LGINPGSE +R+T +RDGIL  FE L A IFTNACGPCIGQWARY DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPE+TA IA AGRLDFNPLTD+L N+ GEEV F+EPTGWELPPK                         DSE              + G KLLIKA+GKCTTDHISMAGPWLR+RGHLDNISNNCLIGAVNAFG KTNFVKNQL GEFGGVPDTARAYKAAG+KT+VVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNF+D+ +FAPNK LTLE+VHADGSK+TI+LNHTYN+AQI
Sbjct:    1 MAFDIDMIKKVYSQMTERVDAARKVVGKPLTLSEKILYSHLWDGTPNTAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKDKVAVPTTVHCDHLIQAKDGAATDLKHANSVSSEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKLSGWTAPKDVILKVAGIVSAKGGTGAIVEYFGEGAKAMSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANQVKEYLTADAEVYANPEQYFDQVIDINLSELGPLLNGPFTPDLSTPVGKTMTEKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGIKMKAELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGEEVMFDEPTGWELPPKGFEVKDNGYLAPDEDGSGVEVKVAPDSERLQLLTPFTPIGNDISGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGMKTNFVKNQLTGEFGGVPDTARAYKAAGIKTVVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANEADYDLIQEDDTFNFIDLNEFAPNKPLTLELVHADGSKDTIVLNHTYNEAQI 738