BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 425/905 (46.96%), Postives = 556/905 (61.44%), Query Frame = 0

Query:   28 RLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEID--YTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            +LP SIRI++ES +RN D  +V E DV+ + NW      + E+PF   RV+LQDFTGVP +VD AAMRDA    G DP+ + P  P DLV+DHS+QVDFA    AL +N  +EF RN+ER+ FLKWG  +F+   ++PPG GIVHQVNLE+LA    +  ++ +PD++VGTDSHTTMINGLGVVGWGVGGIEAEA MLGQP++ ++PEVVG  + G     V +TD+ L IT+ LR  GVVG+FVEFYGPG + LS+ DRATI+NM PEYGAT+G FP+D  +I YLR T R       ++ Y     +    E    D  ++  + LDL +VV + SGPKRPQDR+ V D+   F    +  +   G+    + +N      ++ P                      I     +  L+HGSVLIAAITSCTNTSNPSVML AGL+AKKA   GLTV P++KTSL PGS VVT YL  + +   LD LGF  VGYG  TCIGNSGPL   +       DLVCA VLSGNRNFE RI+   RAN+L SPPLV+AYA+AGR+DID   + LG D N   IYL+D+WP+  E+Q V    + P +F+ +YS + T +E+W  +E+  G  Y W+ +STYI+ PPFFDN S     E + + +   L   G S T DHISPAG I    PA +YL    +   +FNS+GSRRGND IM RGTF N+R+ N    G  G  T +     +                 ++DAA+ Y++  + LI++AG+DYG GSSRDWAAKG  LLGV+AVI +S+ERIHRSNL+GMG++PL + +    + +       FS+  L   L   Q + +   C      S     R DT  E+ YY  GGIL Y++ +++
Sbjct:   33 KLPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRGKNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKN------ITVP---------------------IIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEE-LPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDI---------------MDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVE-LPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKVI 893          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 642.499 bits (1656), Expect = 0.000e+0
Identity = 383/962 (39.81%), Postives = 551/962 (57.28%), Query Frame = 0

Query:    2 DTLRTFENGSFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQS-------------------------------ALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGE-IDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPE-IEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLG-QDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGA-TYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQILDQ 928
            D L+ F+ G   S        I     LP S+R+++E  +R    R  T+  V     W       YE    + RV+LQDFTGVP ++DLAA+R++ + +G  P +V+ L P DLVVDH VQVD+   +                                AL  N + EF RN+ER+ FLKW   AF+   V+PPG G++HQVNLE+LA  V  +  V +PD++VGTD+HTTMINGLGV+GWGVG ++A+A M G PVT   P VVG+ + G +++  T+TDL L IT+ LR  GV G FVEF+GP  + LS+ DRATI+NM PEYGA +GFFP+D  TI YL  +GR +    TVE Y +  +M+   ++ E I +   +E+DL+ ++P VSGPKR +D++ + ++ A F           GYG             LS   G     A  +N D            G    + HG+VL+AAI+SCTNTSNPSVML AGL+AKKA   GL+V   VKTSL PGS VVT YL  + +   L  LGFE VGYGC+TC  NSGP  P+ I  A+   +LVC  +LSGNRNFE R+   IRAN+L SP +V+AYA+AGRIDI+L  +PLG     G  I+LK++WPS  E+QE+    + P +++++YS +   ++ W  +        + W+ DSTYI+ P +         +  ++  + R L   GD++T+DHISPAG+IT +SPA +YL   G+   ++NS+G+RRGN  +M RGTF N+R++N +   + G YT      H+P+             T I++A+  Y+  G  L  I G+++G G+SRDWA +G  LLGV+A++  SF+  +RSNL+  G+LPL       Y+ +K     +  I   +   K + +V +  +   S   + R+D   E D + +GG++  ++++  D+
Sbjct:   14 DLLKDFQVGE-RSYKYYDVTQIQGYEELPYSLRVLLECAVRK---RSETQGQV-----WKDV----YER--ILRRVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYG-------------LSQGQGHV---AFSVNID------------GEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYIKEI----GSNARNWKNMRCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLLNKV-GPYT-----LHLPS----------EVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQITS-ISYESLKGTECLSIDIRD-NITPKCLIDVVVGKA-ERSFKAVVRVDNDYETDVFHAGGVIAQIVSKTKDK 909          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 123.635 bits (309), Expect = 7.567e-29
Identity = 149/518 (28.76%), Postives = 212/518 (40.93%), Query Frame = 0

Query:   90 DLAAMRDAAANAGADPAIVEPL----VPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLA-TGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGL-TVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEAR------IHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPL 595
            D  AM+DA A       I   L    VP  +  DH +Q    G      ++L+     N E Y FL      +   G   PG GI+HQ+ LE+ A  G+L          +VGTDSHT    GLG +  GVGG +A   M   P     P+V+GV L GKL+   +  D+ L++  IL   G  G  VE++G G +++S     TI NM  E GAT   FP +     YL  T RS+   A + + +R  E+   P+ G   Y   +E+DL ++ P V+GP  P                                       DL+TP G   E A               +  G  + ++ G      I SCTN+S   +     ++ K+A A G+ + + F  T   PGS  +   +    L   + + G   +   C  CIG     N        +K      V S NRNF  R       HG     F+ SP + VA +LAG +D D  +D +
Sbjct:  677 DRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIQAQIEGK-----KDLARAKDLNAEVYNFLSTSGAKY-GVGFWKPGSGIIHQIILENYAFPGLL----------MVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRSD--IANLADKYR--EVLLSPDGG-CKYDQVVEIDLNTLEPHVNGPFTP---------------------------------------DLATPIGELGEKA---------------KKNGWPLEVKDG-----LIGSCTNSSYEDMARXTAII-KQALAKGMKSKSNFYIT---PGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWDRQN-------ITKGEKNTIVTSYNRNFTGRNDANPATHG-----FVTSPEMTVALSLAGSLDFDPRSDAI 1098          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81674773|sp|Q5HPJ0.1|ACNA_STAEQ (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 477/927 (51.46%), Postives = 614/927 (66.24%), Query Frame = 0

Query:   11 SFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANW-NAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKD----EVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQILDQ 928
            +++ L  L+  G+A +S+LP SIR+++ES+LR  D   +T+  +K L+ + NA + G  EVPF  +RV+LQDFTGVP VVDLA++R A  + G D   + P VPVDLV+DHSVQVD   +  AL+RN+ +EF RN ERY+FL W  +AF+ +  VPP  GIVHQVNLE+LA  V  +D    +  +PDTLVGTDSHTTMING+GV+GWGVGGIEAEAGMLGQP  F +PEV+GV L   L +G TATDL LR+TE LR  GVVGKFVEF+GPG + L L DRATIANMAPEYGAT GFFP+D E++ Y++ TGR EE    V+ Y +   M+   EK + +YTD ++LDL++V  ++SGPKRPQD I + D+  +F    TA     G+G + S  +  AE++                      ND      GSK  ++ G + IAAITSCTNTSNP VML AGLVAKKA   GL V  FVKTSL PGS+VVT YL  + LQ+ LD LGF  VGYGCTTCIGNSGPL PEIE A+  +DL+  SVLSGNRNFE RIH  ++AN+L SP LVVAYALAG +DIDL N+P+G+ ++G D+YL+D+WPS  E+ + +   + PE+F + Y N+   +E W  I+      YD++ +STYIQ P FF   S+ P  + + L   R +G FGDSVTTDHISPAGAI   +PAGKYLL+H V    FNS+GSRRGN  +M RGTF N+RIKN + PG EGG+T Y+            P+ E      IYDAA+ Y++ G  L+++AG DYGMGSSRDWAAKGT LLGVK VI +S+ERIHRSNL+ MGVLPL F+     + +        S+  ++ +++    V +T        ++   I R D+  E+DYY  GGIL  VL   L Q
Sbjct:   21 TYYDLQTLEEKGLAKISKLPYSIRVLLESVLRQEDDFVITDDHIKALSKFGNAGNEG--EVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMFFDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINF---------------------ND------GSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPG-KIEPLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYW------------PTGE---IMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVD-INEDVQPHDFVNVTAKKENGEIINFKAIVRFDSLVELDYYRHGGILQMVLRNKLAQ 901          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|30173505|sp|P70920.2|ACNA_BRADU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 898.271 bits (2320), Expect = 0.000e+0
Identity = 458/930 (49.25%), Postives = 609/930 (65.48%), Query Frame = 0

Query:   12 FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDE------------VFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQI 925
            ++SLP  +  G+  +S+LP S+++++E+LLRN DGR V ++D+  ++ W  K   ++E+ F  ARV++QDFTGVP VVDLAAMR+A    G D   + PLVPVDLV+DHSV V+F G   A  +N++ E+ +NQERYEFLKWGQ AF  F VVPPG GI HQVNLE+L+  V +K E            V YPD+LVGTDSHTTM+NGL V+GWGVGGIEAEA MLGQP++ L+P VVG  L G + EGVTATDL L +T++LR  GVVGKFVEF+GPG + LS+ D+ATIANMAPEYGAT GFFP+D+  I+YL+ +GR+    A V+ Y +AQ ++   +  +  +T+ L LDLA VVP+++GPKRP+ RI +P                            VAE       GFS   A      ++P+   A+E  G K  + HG V+IAAITSCTNTSNPSV++ AGL+A+ A A GL   P+VKTSL PGS+VV  YL  + LQ  LD++GF  VG+GCTTCIGNSGPL  EI  ++    +V A+VLSGNRNFE R+   ++AN+L SPPLVVA+ALAG +  +L  +PLG+ ++G  +YLKD+WP+  E+   +   +   +F+K Y+++      W  I++    TY WN  STY+Q PP+F+   + P     D+++ R L +FGD +TTDHISPAG+I  TSPAGKYL EH V  A FN +G+RRGN  +M RGTF N+RIKN M  G +G   +     H P  +Q            IYDAA+ YQQ    L++ AG +YG GSSRDWAAKGT LLGV+AVI +SFERIHRSNL+GMGVLPLTF++   +  +        ++ GL G+LK  Q++T     G      + L+ R+DT  E+DYY +GGIL YVL ++
Sbjct:   22 YYSLPTAEKNGLKGISKLPYSMKVLLENLLRNEDGRSVKKADIVAVSKWLRKKSLEHEIAFRPARVLMQDFTGVPAVVDLAAMRNAMQKLGGDAEKINPLVPVDLVIDHSVIVNFFGDNKAFAKNVTEEYKQNQERYEFLKWGQAAFSNFSVVPPGTGICHQVNLEYLSQTVWTKKEKMTVGKKTGTFEVAYPDSLVGTDSHTTMVNGLAVLGWGVGGIEAEACMLGQPLSMLLPNVVGFKLKGAMKEGVTATDLVLTVTQMLRKLGVVGKFVEFFGPGLDHLSVADKATIANMAPEYGATCGFFPVDAAAIDYLKTSGRAAPRVALVQAYAKAQGLFRTAKSADPVFTETLTLDLADVVPSMAGPKRPEGRIALP---------------------------SVAE-------GFSVALANEYKKTEEPAKRFAVE--GKKYEIGHGDVVIAAITSCTNTSNPSVLIGAGLLARNAAAKGLKAKPWVKTSLAPGSQVVAAYLADSGLQAHLDKVGFNLVGFGCTTCIGNSGPLPEEISKSINDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAHALAGSVTKNLAVEPLGEGKDGKPVYLKDIWPTSKEINAFMKKFVTASIFKKKYADVFKGDTNWRKIKTVESETYRWNMSSTYVQNPPYFEGMKKEPEPV-TDIVEARILAMFGDKITTDHISPAGSIKLTSPAGKYLSEHQVRPADFNQYGTRRGNHEVMMRGTFANIRIKNFMLKGADGNIPEGGLTKHWPDGEQ----------MSIYDAAMKYQQEQVPLVVFAGAEYGNGSSRDWAAKGTRLLGVRAVICQSFERIHRSNLVGMGVLPLTFEEGTSWSSLGLKGDEKVTLRGLVGDLKPRQKLTAEIVSGDGSLQRVSLLCRIDTLDELDYYRNGGILHYVLRKL 904          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|81622450|sp|Q9I3F5.1|ACNA_PSEAE (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 897.886 bits (2319), Expect = 0.000e+0
Identity = 506/948 (53.38%), Postives = 631/948 (66.56%), Query Frame = 0

Query:    1 MDTLRTFENGS-------FHSLP-ALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDE----VFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKG-EIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFND-----LFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQ----EVTLTTSCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            +D+L+T  + +       ++SLP A ++LG  D+ +LP+S+++++E+LLR  DG  VT  D+K LA W  +   D E+ +  ARV++QDFTGVP VVDLAAMR A A AG DP  + PL PVDLV+DHSV VD   S+SA ++N+ IE  RN ERY FL+WGQ AF+ F VVPPG GI HQVNLE+L   V +KDE      +PDTLVGTDSHTTMINGLGV+GWGVGGIEAEA MLGQPV+ L+PEV+G  L GKL EG+TATDL L +T++LR  GVVGKFVEFYG G   L L DRATIANMAPEYGAT GFFP+D  T+ YLR +GR E     VE Y + Q +W   EKG E  +TD L LD+  V  +++GPKRPQDR+ + ++ + FN+     L  +S +EG                LS   G +  GA     + D  +D      G    L++G+V+IAAITSCTNTSNPSVM+AAGL+AKKA   GL   P+VK+SL PGS+VVTDY  A  L + LD+LGF+ VGYGCTTCIGNSGPL   IE A+   DL  ASVLSGNRNFE R+H  ++ N+L SPPLVVAYALAG + I+L+ +PLG  ++G  +YLKD+WPS  E+ E I   +  E+F K Y+ +    EKW  I+     TY+W  DSTYIQ PPFF++ +  P     D+   R L V GDSVTTDHISPAG I A SPAG+YL EHGVE   FNS+GSRRGN  +M RGTF N+RIKN M  G EGG T Y            +PS E      IYDAA+ YQ+ G  L+I+AG++YG GSSRDWAAKGT LLGVKAVI +SFERIHRSNL+GMGVLPL F++  D   +K       +I GL GELK       EVT       S  ++ R+DT  E++Y+ +GGIL YVL  +L
Sbjct:    4 LDSLKTLRSLAVDGKTYHYYSLPEAARTLG--DLGKLPMSLKVLLENLLRWEDGSTVTGDDLKALAGWLRERRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASESAFEQNVEIEMQRNGERYAFLRWGQNAFDNFSVVPPGTGICHQVNLEYLGRTVWTKDEDGRTYAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKLREGITATDLVLTVTQMLRKKGVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKLVEAYSKEQGLW--REKGHEPVFTDTLHLDMGEVEASLAGPKRPQDRVALQNVASAFNEFLGLQLHPSSTEEGRL--------------LSEGGGGTAVGANAAFGEIDYQHD------GQTHRLKNGAVVIAAITSCTNTSNPSVMMAAGLLAKKAVEKGLQRKPWVKSSLAPGSKVVTDYFKAAGLTRYLDELGFDLVGYGCTTCIGNSGPLLEPIEKAIQQADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVAYALAGSVRINLSEEPLGTGKDGQPVYLKDIWPSQKEIAEAI-QKVDTEMFHKEYAEVFAGDEKWQAIQVPQSDTYEWQADSTYIQHPPFFEHIAEAPPAIA-DVEQARVLAVLGDSVTTDHISPAGNIKADSPAGRYLREHGVEPKDFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLY------------VPSGEKLA---IYDAAMRYQEDGTPLVIVAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFENGQDRKSLKLTGKEVLNIRGLGGELKPHMPLSVEVTREDGSQDSFKVLCRIDTLNEVEYFKAGGILHYVLRSML 910          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|543763|sp|P37032.1|ACON_LEGPH (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=IP210; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Major iron-containing protein; Short=MICP; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 897.116 bits (2317), Expect = 0.000e+0
Identity = 483/923 (52.33%), Postives = 610/923 (66.09%), Query Frame = 0

Query:   11 SFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDE----VFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELK--TMQEVTLTTSCG--LSLPLIARLDTPAEIDYYLSGGILPYVLNQI 925
            +++SL   ++     ++RLP S+++++E+LLR  DG  VT  D+K +A+W       +E+ F   RV++QDFTGVP VVDLAAMR A    G +   + PL PVDLV+DHSV VD   S  AL+ N  IE  RN+ERYEFL+WGQ+AF  F VVPPG GI HQVNLE+L   V + +       YPDTLVGTDSHTTMINGLGV+GWGVGGIEAEA MLGQPV+ L+PEV+G  L GKL EG+TATDL L +T++LR  GVVGKFVEFYGPG   L L DRATI+NMAPEYGAT GFFP+D ETI YL  TGR +   A VE Y +AQ MW   +  E  +TD L LDL SV P+++GPKRPQD++++  L  +FN+     ++ G    +  T                                     +     ++HG V+IAAITSCTNTSNPSV++AAGLVAKKA   GL   P+VK+SL PGS+VVTDYL    LQ  LDQLGF  VGYGCTTCIGNSGPL  +I   +   DLV +SVLSGNRNFE R+H  +RAN+L SPPLVVAYAL G    DL+ +P+GQD+ GND+YLKD+WPS  E+   + + +   +F+K Y+ +      W  I++++G TY+WN DSTYIQ PPFF+N S  P    + +     L +FGDS+TTDHISPAG+I A+SPAG YL   GV++  FNS+GSRRGN  +M RGTF N+RI+N MTPG EGG T+Y            +P+ E      IYDAA+ YQ++ + L+IIAG++YG GSSRDWAAKGT LLGVKAVIT+SFERIHRSNLIGMG+LPL FK+      +K   S   SI  +S +L    M  VT+    G    +  + R+DT  E++YY +GGIL YVL +I
Sbjct:   22 NYYSLKEAENKHFKGINRLPYSLKVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDLAAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYEFLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENDGQLYAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQMLRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGRDKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSLPVEFNNFL---IEVGKEKEKEKT----------------------------------FAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLVAKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNSGPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCSDLSREPIGQDKEGNDVYLKDIWPSNEEIAAEV-AKVSGTMFRKEYAEVFKGDAHWQAIQTSSGQTYEWNPDSTYIQHPPFFENLSLKPEPL-KPIKQAYVLALFGDSITTDHISPAGSIKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTRY------------VPTGETMS---IYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIE-ISDKLTPGAMVPVTIERQDGDIEKIETLCRIDTADELEYYKNGGILQYVLRKI 889          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391209|sp|Q6GH55.1|ACNA_STAAR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 895.19 bits (2312), Expect = 0.000e+0
Identity = 477/926 (51.51%), Postives = 610/926 (65.87%), Query Frame = 0

Query:   11 SFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKD----EVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQILDQ 928
            +++ L A++  GI  VS+LP SIR+++ESLLR  D   +T+  +K L+ +  K   + EVPF  +RV+LQDFTGVP VVDLA++R A  + G D   + P VPVDLV+DHSVQVD   +  AL+RN+ +EF RN ERY+FL W  +AF+ +  VPP  GIVHQVNLE+LA+ V  +D    +  +PDTLVGTDSHTTMING+GV+GWGVGGIEAEAGMLGQP  F +PEV+GV L   L +G TATDL LR+T+ LR  GVVGKFVEF+GPG + L L DRATIANMAPEYGAT GFFP+D E++ Y++ TGRS+E  A V+ Y +   M+   EK + +YTD +ELDL++V  ++SGPKRPQD I + D+ + F +  TA     G+G + S  +  AE++                              GSK  ++ G + IAAITSCTNTSNP VML AGLVAKKA   GL V  +VKTSL PGS+VVT YL    LQ  LD LGF  VGYGCTTCIGNSGPL PEIE A+  +DL+  SVLSGNRNFE RIH  ++AN+L SP LVVAYALAG +DIDL N+P+G+  +G D+YLKD+WPS  E+ + + S + PE+F + Y+N+   +E W  I+      YD++ +STYIQ P FF   S+ P T    L   R +G FGDSVTTDHISPAGAI   +PAGKYL +H V   +FNS+GSRRGN  +M RGTF N+RIKN + PG EGG+T Y+            P+ E      I+DAA+ Y++ G  L+++AG DYGMGSSRDWAAKGT LLGVK VI +S+ERIHRSNL+ MGVLPL FK     D +    +   S+  +   ++    V +T        +    + R D+  E+DYY  GGIL  VL   L Q
Sbjct:   21 TYYDLKAVEEQGITKVSKLPYSIRVLLESLLRQEDDFVITDDHIKALSQF-GKDGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKSSFENSVTAPAGNQGHGLDKSEFDKKAEINFK---------------------------DGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRGAGLQPYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGSVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYNNNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTI-VPLNGLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYW------------PTNE---VMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVN-IDENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|54036686|sp|P63434.1|ACNA_STAAW (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|54036700|sp|P99148.1|ACNA_STAAN RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|54040671|sp|P63433.1|ACNA_STAAM RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|60391208|sp|Q6G9K9.1|ACNA_STAAS RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein >gi|81694556|sp|Q5HG69.1|ACNA_STAAC RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 893.649 bits (2308), Expect = 0.000e+0
Identity = 477/926 (51.51%), Postives = 609/926 (65.77%), Query Frame = 0

Query:   11 SFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKD----EVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQILDQ 928
            +++ L A++  GI  VS LP SIR+++ESLLR  D   +T+  +K L+ +  K   + EVPF  +RV+LQDFTGVP VVDLA++R A  + G D   + P VPVDLV+DHSVQVD   +  AL+RN+ +EF RN ERY+FL W  +AF+ +  VPP  GIVHQVNLE+LA+ V  +D    +  +PDTLVGTDSHTTMING+GV+GWGVGGIEAEAGMLGQP  F +PEV+GV L   L +G TATDL LR+T+ LR  GVVGKFVEF+GPG + L L DRATIANMAPEYGAT GFFP+D E++ Y++ TGRS+E  A V+ Y +   M+   EK + +YTD +ELDL++V  ++SGPKRPQD I + D+ + F +  TA     G+G + S  +  AE++                              GSK  ++ G + IAAITSCTNTSNP VML AGLVAKKA   GL V  +VKTSL PGS+VVT YL    LQ  LD LGF  VGYGCTTCIGNSGPL PEIE A+  +DL+  SVLSGNRNFE RIH  ++AN+L SP LVVAYALAG +DIDL N+P+G+  +G D+YLKD+WPS  E+ + + S + PE+F + Y+N+   +E W  I+      YD++ +STYIQ P FF   S+ P T    L   R +G FGDSVTTDHISPAGAI   +PAGKYL +H V   +FNS+GSRRGN  +M RGTF N+RIKN + PG EGG+T Y+            P+ E      I+DAA+ Y++ G  L+++AG DYGMGSSRDWAAKGT LLGVK VI +S+ERIHRSNL+ MGVLPL FK     D +    +   S+  +   ++    V +T        +    + R D+  E+DYY  GGIL  VL   L Q
Sbjct:   21 TYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQF-GKDGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKSSFENSVTAPAGNQGHGLDKSEFDKKAEINFK---------------------------DGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYNNNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTI-VPLNGLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYW------------PTNE---VMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVN-IDENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|88909584|sp|Q8FTA8.2|ACNA_COREF (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 892.878 bits (2306), Expect = 0.000e+0
Identity = 459/930 (49.35%), Postives = 613/930 (65.91%), Query Frame = 0

Query:   26 VSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPA-------GFSP---------------EG-ALLLNSDDDPSNDVAIET-QGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLS----GELKTMQEVTLTTSCG--LSLPLIARLDTPAEIDYYLSGGILPYVLNQI 925
            + +LP S++++ E+LLR  DG  +TE  ++ +ANW+A +    E+ FT ARV++QDFTGVP VVDLA MR+A    G DP  V PL P ++V+DHSV V+  G   AL++N+ IE+ RN+ERY+FL+WG +AF  F VVPPG GIVHQVN+E+LA  V   D + YPDT +GTDSHTTM NGLG++GWGVGGIEAEA MLGQPV+ L+P VVG  L G++  GVTATD+ L ITE+LR HGVV KFVEFYG G +++ L +RATI NM+PE+G+T   FP+D ETI YLR TGR EE  A VE Y +AQ MW   +  E +Y++ LELDL++VVP+++GPKRPQDRI + +   +F +   A              ND  ++D S PA       GF P               EG +   N++  PSN V + + QG +  + HG V IA+ITSCTNTSNPSVM+ AGL+A+KA   GL   P+VKT   PGS+VV  Y     L K+L+ LGF   G+GCTTCIGNSGPL  EI  A+   DL   +VLSGNRNFE RI   ++ N+L SP +V+AYA+AG +D D  N+ LGQDQ+GND++LKD+WPS  E++E I +A+  E+++  Y+++    ++W  ++  +G T++W+ +STYI+  P+FD  +  P     D+ + R L   GDSVTTDHISPA +I   +PA +YL  HGVE+  +NS GSRRGN  +M RGTF N+R++N +   I GGYT+ F             + E     FIYDA V Y+++G  L+++AG++YG GSSRDWAAKGT LLGV+AVIT+SFERIHRSNLIGMGV+PL F +   ++ +    + TF ITGL+    G      +VT T   G  +    + R+DTP E DY+  GGIL YVL Q+
Sbjct:   32 MEKLPYSLKVLGENLLRTEDGANITEEHIEAIANWDASADPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVKTLGGDPDKVNPLNPAEMVIDHSVIVEAFGRPDALEKNVEIEYERNEERYQFLRWGSEAFSNFRVVPPGTGIVHQVNIEYLARVVFDNDGLAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGNGVKSVPLANRATIGNMSPEFGSTCAMFPIDEETIKYLRLTGRPEEQIALVEAYAKAQGMWLEQDAPEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFREDLKAYT------------NDPVQVDQSIPAKRMANEGGFQPGSTSDLDNYNASWPGEGESAAANAEGRPSNPVTVVSPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEALGFYLSGFGCTTCIGNSGPLPEEISEAINEHDLAATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEETIQAAISRELYEADYADVFKGDKQWQELDIPSGKTFEWDENSTYIRKAPYFDGMTAEPQPV-TDIENARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDAHGVERQDYNSLGSRRGNHEVMMRGTFANIRLQNQLV-DIAGGYTRDF-------------TQEGGPQAFIYDACVNYKEAGIPLVVLAGKEYGTGSSRDWAAKGTNLLGVRAVITESFERIHRSNLIGMGVVPLQFPEGESHESLGLDGTETFDITGLTALNEGTTPKTVKVTATKENGEKVEFDAVVRIDTPGEADYFRHGGILQYVLRQM 934          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|122426093|sp|Q1RKD5.1|ACNA_RICBR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 889.412 bits (2297), Expect = 0.000e+0
Identity = 459/908 (50.55%), Postives = 601/908 (66.19%), Query Frame = 0

Query:   26 VSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQ----GSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQI 925
            + +LP S+R++ E++LR   G K    ++     W      D E+ F  ARV++QDFTGVP +VDLAAMRDA    G DP  + PL+PVDLV+DHSV VD   S S+ D+N+++E  RN ERY+FLKWGQQAF  F VVPPG GI HQVNLE+LA  V   + V YPD+LVGTDSHTTM+NGL V+GWGVGGIEAEA MLGQP+T ++PEV+GV L GKL    TATDL L+ITE+LR   VVGKFVEFYG G +A+++ DRATI+NMAPEYGAT GFFP+D ETI YL  TGR +E    VE Y +AQ++W   +    +YTD LELDL+ V  +++GP+RPQDR+++ D+ +             G+ +E ST              FS             SN+++I+T+         + +G V+IAAITSCTNTSNPSVM+ A L+AKKA   GL V P+VKTSL PGS+VVT+YL ++ L + LDQLGF  VGYGCTTCIGNSGPLNPEIE  +    LV ASVLSGNRNFE RI+   +A++L SP LVVAYAL+G ++IDLTN PLG++  G D+YLKD+WPS  E+ +VI +++   +F + YS++ + +++W  +E  + + Y W+  STYI  PP+F+N     +   +D+   R L +FGDS+TTDHISPAG+I+ TSPA KYL +H +    FNS+GSRRGN  +M RGTF N+RIKN M  G+EGG+T              I   +N + T IYDAA+ Y+ +G   +I AG++YG GSSRDWAAKG  LLGVKAVI +SFERIHRSNL+GMGVLPL F +      +K   S +  I GL+  +K    V            ++ LI ++ T  EI+Y   G I+ +V+  +
Sbjct:   36 LKKLPYSLRVLFENVLRT--GSK---QNLMVFKEWLKNKKSDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYASGSSFDKNVAMEMRRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVWHSNGVAYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGTATATDLVLKITEMLRKKKVVGKFVEFYGEGLKAMTIADRATISNMAPEYGATCGFFPIDQETIKYLELTGRDKEQIKLVEEYAKAQDLWCNFDDAA-EYTDILELDLSEVTSSLAGPRRPQDRVNLGDVSS-------------GFKKELST--------------FS-------------SNNISIDTKHAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKAIEQGLKVKPWVKTSLAPGSKVVTEYLKSSGLNQYLDQLGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGSLNIDLTNHPLGKNDKGRDVYLKDIWPSKEEIDKVIANSINSSMFVEKYSDIFSGTKEWQSLEVTSSSNYAWDKSSTYINNPPYFENIGSKNSI--KDIKSARILAIFGDSITTDHISPAGSISKTSPAAKYLTDHQISPIDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFT--------------INQLKNMQQT-IYDAAMDYKANGVSAVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGVLPLIFTNNMTRFDLKLDGSESIDIIGLNEHIKPYNSVKCIIKKQNGEMQTIDLILQIFTDNEINYIKHGSIMHFVVENL 880          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|166215073|sp|P25516.3|ACNA_ECOLI (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=RNA-binding protein; AltName: Full=Stationary phase enzyme)

HSP 1 Score: 885.56 bits (2287), Expect = 0.000e+0
Identity = 486/942 (51.59%), Postives = 615/942 (65.29%), Query Frame = 0

Query:    2 DTLRTFENG-SFHSLP-ALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSK----DEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLS----GELKTMQ-----EVTLTTSCGLS--LPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            DTL+  +    ++SLP A KSLG  D++RLP S+++++E+LLR  DG  VTE D+  LA W   +  D E+ +  ARV++QDFTGVP VVDLAAMR+A    G D A V PL PVDLV+DHSV VD  G   A + N+ +E  RN ERY FLKWG+QAF  F VVPPG GI HQVNLE+L   V S+    + + YPDTLVGTDSHTTMINGLGV+GWGVGGIEAEA MLGQPV+ L+P+VVG  L GKL EG+TATDL L +T++LR HGVVGKFVEFYG G ++L L DRATIANM+PEYGAT GFFP+D+ T++Y+R +GRSE+    VE Y +AQ MW  P    I +T  LELD+  V  +++GPKRPQDR+ +PD+                                  P  F+    L +N+       V     G +  L  G+V+IAAITSCTNTSNPSV++AAGL+AKKA  LGL   P+VK SL PGS+VV+DYL    L   LD+LGF  VGYGCTTCIGNSGPL   IE A+   DL   +VLSGNRNFE RIH  ++ N+L SPPLVVAYALAG ++I+L ++P+G D+ G+ +YLKD+WPS  E+   +   +  E+F+K Y+ +   + +W GI      TY W  DSTYI+L PFFD    TPA   +D+   R L + GDSVTTDHISPAG+I   SPAG+YL   GVE+  FNS+GSRRGN  +M RGTF N+RI+N M PG+EGG T++        PD D+ S        IYDAA+ Y+Q    L +IAG++YG GSSRDWAAKG  LLG++ VI +SFERIHRSNLIGMG+LPL F         + +   T  +TG      G+L+ +Q      VTLT + G    +P   R+DT  E+ YY + GIL YV+  +L
Sbjct:   11 DTLQAKDKTYHYYSLPLAAKSLG--DITRLPKSLKVLLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPI-FTSTLELDMNDVEASLAGPKRPQDRVALPDV----------------------------------PKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGDPVYLKDIWPSAQEIARAV-EQVSTEMFRKEYAEVFEGTAEWKGINVTRSDTYGWQEDSTYIRLSPFFDEMQATPAPV-EDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHL-------PDSDVVS--------IYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFP--------QGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILHYVIRNML 890          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|88909585|sp|Q8NQ98.2|ACNA_CORGL (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 885.174 bits (2286), Expect = 0.000e+0
Identity = 459/931 (49.30%), Postives = 609/931 (65.41%), Query Frame = 0

Query:   26 VSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKF-NDLFTASVDEGGYGREASTRNDVAELDLSTPA-------GFSPEG----------------ALLLNSDDDPSNDVAIET-QGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLS----GELKTMQEVTLTTSCG--LSLPLIARLDTPAEIDYYLSGGILPYVLNQI 925
            + +LP S++++ E+LLR  DG  +T   ++ +ANW+A S    E+ FT ARV++QDFTGVP VVDLA MR+A A  G DP  V PL P ++V+DHSV V+  G   AL +N+ IE+ RN+ERY+FL+WG ++F  F VVPPG GIVHQVN+E+LA  V   + + YPDT +GTDSHTTM NGLG++GWGVGGIEAEA MLGQPV+ L+P VVG  L G++  GVTATD+ L ITE+LR HGVV KFVEFYG G +A+ L +RATI NM+PE+G+T   FP+D ET  YLR TGR EE  A VE Y +AQ MW   +  E +Y++ LELDL++VVP+++GPKRPQDRI + +   +F  DL T +             +D   +D S PA       G  PEG                +L   ++  PS  V + + QG +  + HG V IA+ITSCTNTSNPSVM+ AGL+A+KA   GL   P+VKT   PGS+VV  Y     L K+L+ +GF   G+GCTTCIGNSGPL  EI AA+   DL   +VLSGNRNFE RI   ++ N+L SP +V+AYA+AG +D D  N+ LGQDQ+GND++LKD+WPS  E+++ I  A+  E+++  Y+++    ++W  ++  TG T++W+ +STYI+  P+FD     P     D+   R L   GDSVTTDHISPA +I   +PA +YL EHGVE+  +NS GSRRGN  +M RGTF N+R++N +   I GGYT+ F             + E A   FIYDA+V Y+ +G  L+++ G++YG GSSRDWAAKGT LLG++AVIT+SFERIHRSNLIGMGV+PL F     ++ +    + TF ITGL+    GE     +VT T   G  +    + R+DTP E DYY  GGIL YVL Q+
Sbjct:   32 MEKLPYSLKVLGENLLRTEDGANITNEHIEAIANWDASSDPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVAALGGDPNDVNPLNPAEMVIDHSVIVEAFGRPDALAKNVEIEYERNEERYQFLRWGSESFSNFRVVPPGTGIVHQVNIEYLARVVFDNEGLAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPVGVTATDVVLTITEMLRDHGVVQKFVEFYGSGVKAVPLANRATIGNMSPEFGSTCAMFPIDEETTKYLRLTGRPEEQVALVEAYAKAQGMWLDEDTVEAEYSEYLELDLSTVVPSIAGPKRPQDRILLSEAKEQFRKDLPTYT-------------DDAVSVDTSIPATRMVNEGGGQPEGGVEADNYNASWAGSGESLATGAEGRPSKPVTVASPQGGEYTIDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAEKGLKSKPWVKTICAPGSQVVDGYYQRADLWKDLEAMGFYLSGFGCTTCIGNSGPLPEEISAAINEHDLTATAVLSGNRNFEGRISPDVKMNYLASPIMVIAYAIAGTMDFDFENEALGQDQDGNDVFLKDIWPSTEEIEDTIQQAISRELYEADYADVFKGDKQWQELDVPTGDTFEWDENSTYIRKAPYFDGMPVEPVAV-TDIQGARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDEHGVERHDYNSLGSRRGNHEVMMRGTFANIRLQNQLV-DIAGGYTRDF-------------TQEGAPQAFIYDASVNYKAAGIPLVVLGGKEYGTGSSRDWAAKGTNLLGIRAVITESFERIHRSNLIGMGVVPLQFPAGESHESLGLDGTETFDITGLTALNEGETPKTVKVTATKENGDVVEFDAVVRIDTPGEADYYRHGGILQYVLRQM 934          

BLAST of aconitate hydratase vs. nr
Match: gi|1110141708|emb|SHJ40866.1| (aconitase [Rubritalea squalenifaciens DSM 18772])

HSP 1 Score: 1439.48 bits (3725), Expect = 0.000e+0
Identity = 720/951 (75.71%), Postives = 797/951 (83.81%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAG---------FSP-----EGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTS----CGLSLPLIARLDTPAEIDYYLSGGILPYVLNQILDQ 928
            +DT+RTF+ GS     ++SLPAL+  G   +SRLP+SIRIV+ESLLRN DGRKVTE DVKNLANWNA +PG+YEVPFTVARVVLQDFTGVPLVVDLAAMR+AAA  GADPA+VEPLVPVDLV+DHSVQVDFAGSQ AL+RNL IEFIRN+ERYEFLKWGQQAF+TF VVPPGIGIVHQVNLE+LA GVL K+  +Y D+LVGTDSHTTMINGLGVVGWGVGGIEAEA MLGQPVTFLVPEVVGVYLHG+LAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGA+ALSLPDRATIANMAPEYGATMGFFP+D E+++YLRGTGRS+E+C TVENYF+AQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLG KFN+LFTAS  +GG+GR   TRN   ++ +  PAG         F+P     +  +  N     + D A         L+HGSVLIAAITSCTNTSNPSVMLA+GLVAKKANALGLTV P+VKTSLGPGSRVVTDYLN T LQ ELDQLGF+TVGYGCTTCIGNSGPL+P IE A+   DLVCASVLSGNRNFEAR+HGSIRANFLMSPPLVVAYALAGR+D+DL +DP+  D+NGN +YLKD+WP+  EL+EVI SAL PEVF  LYSNLDTA++ WV I S TG  Y W+  STYIQ PPFF+ FSRTPATEG+D+ D RPLG+FGDSVTTDHISPAGAI  TSPAG+YLLE+GVEKA FNSFGSRRGNDR+MTRGTF NVRIKN M  G EGGYTQY+G   VPAPD++I      K  FIYDAA AY + G  LI+I GEDYGMGSSRDWAAKGT LLGV AVITKSFERIHRSNLIGMGVLPL FKDK+DYDK+ ++   TFSI GL+GELK  Q  TL  S        +PLI RLDTPAEIDYYLSGGILPYVLNQILD 
Sbjct:    3 LDTIRTFQTGSGQEGKYNSLPALQEQGFDKISRLPVSIRIVLESLLRNCDGRKVTEEDVKNLANWNAAAPGNYEVPFTVARVVLQDFTGVPLVVDLAAMRNAAAETGADPAVVEPLVPVDLVIDHSVQVDFAGSQQALNRNLQIEFIRNKERYEFLKWGQQAFDTFSVVPPGIGIVHQVNLEYLAKGVLEKEGTYYFDSLVGTDSHTTMINGLGVVGWGVGGIEAEAAMLGQPVTFLVPEVVGVYLHGELAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAKALSLPDRATIANMAPEYGATMGFFPVDEESVSYLRGTGRSKEICETVENYFKAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGNKFNELFTASTADGGFGRTEDTRNISVDIHMPEPAGVAIGSLLDDFTPGAEMDKAEMTTNRPTPDAVDAAEAFDPIDTQLKHGSVLIAAITSCTNTSNPSVMLASGLVAKKANALGLTVPPYVKTSLGPGSRVVTDYLNKTGLQTELDQLGFQTVGYGCTTCIGNSGPLHPAIEGAIKEGDLVCASVLSGNRNFEARVHGSIRANFLMSPPLVVAYALAGRVDLDLAHDPISTDKNGNLVYLKDIWPTIDELKEVIDSALVPEVFNTLYSNLDTANQNWVDIVSDTGDIYSWDDKSTYIQNPPFFEGFSRTPATEGKDITDARPLGIFGDSVTTDHISPAGAIVPTSPAGQYLLENGVEKASFNSFGSRRGNDRVMTRGTFANVRIKNTMCEGTEGGYTQYYGPQEVPAPDKEIAPVNGGKPAFIYDAAQAYAKDGTDLIVIGGEDYGMGSSRDWAAKGTRLLGVSAVITKSFERIHRSNLIGMGVLPLNFKDKSDYDKVANLSDATFSIIGLTGELKASQNATLVVSKADGSSFEVPLIVRLDTPAEIDYYLSGGILPYVLNQILDN 953          

BLAST of aconitate hydratase vs. nr
Match: gi|737638557|ref|WP_035608588.1| (aconitate hydratase [Haloferula sp. BvORR071])

HSP 1 Score: 1358.58 bits (3515), Expect = 0.000e+0
Identity = 684/956 (71.55%), Postives = 781/956 (81.69%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDP-----SNDVAIETQG---------------SKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTT----SCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQILD 927
            MDTLRTF+ GS     F+SLPAL   G  ++S+LP+SIRIV+ES+LR +DGRKVTE DV NLAN+NAK+PG+YE+PFTVAR+VLQDFTGVPL+VD+AAMRDAA   GA PA +EPLVPVDLVVDHSVQVDFAGSQ ALDRN++IEFIRN+ERYEFLKWGQQAFETF VVPPGIGIVHQVNLE LA GVLSKD V+YPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHG+L EGVTATDLTLRIT++LR HGVVGKFVEF+GPGA+ALSLPDRATIANMAPEYGATMGFFP+D ETINYLRGTGR+EELC TVENY++AQ ++GIP+KGE +YTD LELDLAS+VPAV+GPKRPQDRIDV  L   F+ LFTA V  GG+G  AS R     L +   AG S +  L  +S+  P      N+V + +                   + L HGS+LIAAITSCTNTSNPSVM+AAGLVAKKANALGLTVAP+VKTSL PGSRVVTDY  AT LQKELDQLGF+TVGYGCTTCIGNSGPL+P IE A++   LVCASVLSGNRNFEAR+HGSI+ANFLMSPPLVVAYALAGR+D+DLT +PLG D++GN ++LKDLWP+  E++E + +ALKP V+QKLY +L +A+ KW  I    GATY W+  STYIQ PPFFD      AT   D+++ RPLG+FGDSVTTDHISPAG+I ATSPAGKYLL+HGVEKA+FNS+G+RRGNDR+MTRGTF NVRIKNLM PG+EGGYTQYFG A V  PDQ I   + AK TFIYDA++AYQ  G KLIII GEDYGMGSSRDWAAKGT LLGV AV+TKSFERIHRSNLIGMGVLPL F   ADYD++K +    F ITG+ GELK MQ+  L         +  PL  RLDTPAE+DYYL+GGILPYVL+QIL+
Sbjct:    1 MDTLRTFKTGSGADGKFYSLPALAEQGFPNLSKLPVSIRIVLESVLRCVDGRKVTEKDVANLANYNAKTPGEYEIPFTVARIVLQDFTGVPLLVDVAAMRDAAVKRGAQPAKIEPLVPVDLVVDHSVQVDFAGSQLALDRNMAIEFIRNRERYEFLKWGQQAFETFSVVPPGIGIVHQVNLEFLAKGVLSKDGVYYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGELKEGVTATDLTLRITQLLRKHGVVGKFVEFHGPGAKALSLPDRATIANMAPEYGATMGFFPVDEETINYLRGTGRTEELCTTVENYYKAQGLFGIPDKGECEYTDLLELDLASIVPAVAGPKRPQDRIDVTALRETFDTLFTAPVANGGFGLPASDREKRVRLTMREKAGVSVDSLLSTDSNHQPFEGGERNEVEMVSNRPTPDQVNEDLFAGGPQDMELAHGSILIAAITSCTNTSNPSVMIAAGLVAKKANALGLTVAPYVKTSLAPGSRVVTDYFEATGLQKELDQLGFQTVGYGCTTCIGNSGPLHPSIEGAISEGSLVCASVLSGNRNFEARVHGSIKANFLMSPPLVVAYALAGRVDLDLTTEPLGNDKDGNPVFLKDLWPTHTEVKEQLAAALKPAVYQKLYGDLASANPKWAEISGTAGATYGWDEKSTYIQSPPFFDG----GATVTGDILNARPLGIFGDSVTTDHISPAGSIKATSPAGKYLLDHGVEKAEFNSYGARRGNDRVMTRGTFANVRIKNLMCPGVEGGYTQYFGPAMVSEPDQKIDPVDGAKPTFIYDASMAYQADGTKLIIIGGEDYGMGSSRDWAAKGTRLLGVSAVVTKSFERIHRSNLIGMGVLPLNFIHAADYDRVKSLAEARFDITGIGGELKPMQDALLIAHLPDHSRMEFPLKVRLDTPAEVDYYLAGGILPYVLDQILE 952          

BLAST of aconitate hydratase vs. nr
Match: gi|1240615348|gb|PAW69423.1| (aconitate hydratase [Verrucomicrobiae bacterium Tous-C4TDCM])

HSP 1 Score: 1333.55 bits (3450), Expect = 0.000e+0
Identity = 672/955 (70.37%), Postives = 775/955 (81.15%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNS-----DDDPSNDVAIETQG---------------SKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTT----SCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            MDT+RTF  GS     F+SLPAL   G  ++++LP+SIRIV+ES+LR +DGRKV+E DV NLAN+NAK+PG+YE+PFTVAR+VLQDFTGVPL+VD+AAMRDAA   GA P  +EPLVPVDLVVDHSVQVDF+GSQ ALDRN++IEFIRN+ERYEFLKWGQQAF+TF VVPPGIGIVHQVNLE LA GVL K+ V+YPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYL G+L EGVTATDLTLRIT++LR HGVVGKFVEF+GPGA ALSLPDRATIANMAPEYGATMGFFP+D ETI YLRGTGRSEELC TVENY++AQ ++GIP+KG+ DYTD LELDLAS+VPAV+GPKRPQDRIDV  L   F+ LFTA V  GG+G  A  R     L +   AG S +  L  ++     +  P N+  + +                   + L HGS+LIAAITSCTNTSNPSVM+AAGLVAKKANALGLTVAP+VKTSL PGSRVVT+Y  AT LQKELDQLGF+TVGYGCTTCIGNSGPL+P IE A+   +LVCASVLSGNRNFEAR+HGSI+ANFLMSPPLVVAYALAGR+D+DLT +PLG D++GN ++LKDLWP+  E++E + +ALKP V+QKLY +LD+A+ KW  I  +TGATY W+  STYIQ PPFFD      AT   D+ + R LG+FGDSVTTDHISPAG+I ATSPAGKYL++HGV KA+FNS+G+RRGNDR+MTRGTF NVRIKNLM PG+EGGYTQYFGDA V APD  I + E AK TFIYDA++AYQ  G KLIII G+DYGMGSSRDWAAKGT LLGV AV+TKSFERIHRSNLIGMGVLPL F +  DYDK+K +    F ITG+ GELK MQ+  L         L +PL  RLDTPAE+DYYL+GGILPYVL+QIL
Sbjct:    1 MDTVRTFTTGSGAEGKFYSLPALAEQGFPNLAKLPVSIRIVLESVLRCVDGRKVSEKDVANLANYNAKAPGEYEIPFTVARIVLQDFTGVPLLVDVAAMRDAAVKRGASPEKIEPLVPVDLVVDHSVQVDFSGSQLALDRNMAIEFIRNRERYEFLKWGQQAFKTFSVVPPGIGIVHQVNLEFLAKGVLEKNGVYYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLTGELKEGVTATDLTLRITQVLRKHGVVGKFVEFHGPGATALSLPDRATIANMAPEYGATMGFFPVDGETIAYLRGTGRSEELCTTVENYYKAQGLFGIPDKGDCDYTDLLELDLASIVPAVAGPKRPQDRIDVTALRESFDTLFTAPVSAGGFGFAADQRGRRVNLSMREKAGVSIDSLLSTDAKHKAFEGGPRNETEMVSNRPTPDTVDPDLFAGGPRDLELGHGSILIAAITSCTNTSNPSVMIAAGLVAKKANALGLTVAPYVKTSLAPGSRVVTEYFEATDLQKELDQLGFQTVGYGCTTCIGNSGPLHPAIEGAIQEGNLVCASVLSGNRNFEARVHGSIKANFLMSPPLVVAYALAGRVDLDLTTEPLGNDKDGNPVFLKDLWPTHGEVKEQLAAALKPSVYQKLYGDLDSANPKWAEISGSTGATYAWDEKSTYIQSPPFFDG----GATVVGDIHNARALGIFGDSVTTDHISPAGSIKATSPAGKYLIDHGVAKAEFNSYGARRGNDRVMTRGTFANVRIKNLMCPGVEGGYTQYFGDAEVSAPDTTIEALEGAKPTFIYDASMAYQADGAKLIIIGGDDYGMGSSRDWAAKGTRLLGVSAVVTKSFERIHRSNLIGMGVLPLNFINADDYDKVKHLADARFDITGIHGELKPMQDALLVCHLPDHSRLEIPLKVRLDTPAEVDYYLAGGILPYVLDQIL 951          

BLAST of aconitate hydratase vs. nr
Match: gi|1154910234|ref|WP_078816083.1| (aconitate hydratase [Prosthecobacter debontii] >gi|1152225334|emb|SKB08587.1| aconitase [Prosthecobacter debontii])

HSP 1 Score: 1228.39 bits (3177), Expect = 0.000e+0
Identity = 623/934 (66.70%), Postives = 724/934 (77.52%), Query Frame = 0

Query:   12 FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGRE-----ASTRNDVAELDLSTPAGFSPEGAL-------LLNSDDD--PSNDVAIETQ-GSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTS----CGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            ++SLP L+  G+  +S+LP+SIRIV+ESLLRNLDG+KV E DVKNLANWNAK+PGDYE+PFTVAR+VLQDFTGVPL+VDLAAMR A A  G +  ++EPLVPVDLVVDHSVQVDFAG+Q AL++NL++EF RN+ERYEFLKWG+QAF+TF VVPPGIGIVHQVNLE+LA GVL KD VFYPDTLVGTDSHTTMINGLGVV WGVGGIEAEAGMLGQPVTFLVPEVVGVYL G L EGVTATDL L  T++LR HGVVGKFVEFYGPGAE+L LPDRATIANMAPEYGATMGFFP+D E +NYLRGTGRSEELC T +NY+ AQ M+GIP+KGE++YT DLELDL  + P+V+GPKRPQDRI V  L + +N++       GGYG+          N  A LD + P G     AL        + SD    P  +V ++++ G K A+ HGSVLIAAITSCTNTSNPSVMLAAGL+AKKANA GLTV P VKTSLGPGSRVVTDYLN T LQ ELD+LGF+TVGYGCTTCIGNSGPL+  IE  + ++D+V ASVLSGNRNFEAR+H SI+ANFLMSPPLVVAYA+AG +DIDL+ D +     G  +YLKD+WPS  E+Q+ + SAL PEVF+ LY++  + + KW  I  +TG  YDWN  STYIQ PPFF++FS  P  +  +++  RPLG+FGDSVTTDHISPAGAI  TSPAG++L E+ V +A FNS+GSRRGNDR+MTRGTF NVRIKNLM  G EGG T        PA               IYDAA AY  +G   III GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNL+GMGVLP  FKDKADYDK+KD+   TFSI G+S + K   E TL  +        +PL+ R+DTP EIDYY +GGILPYVL QIL
Sbjct:   17 YYSLPELEKQGVGPISKLPVSIRIVLESLLRNLDGKKVFEQDVKNLANWNAKNPGDYEIPFTVARIVLQDFTGVPLLVDLAAMRSAVAKMGKNTKMIEPLVPVDLVVDHSVQVDFAGTQEALNQNLALEFERNRERYEFLKWGEQAFDTFKVVPPGIGIVHQVNLEYLAKGVLDKDGVFYPDTLVGTDSHTTMINGLGVVAWGVGGIEAEAGMLGQPVTFLVPEVVGVYLTGSLREGVTATDLALHCTQMLRKHGVVGKFVEFYGPGAESLPLPDRATIANMAPEYGATMGFFPIDEECVNYLRGTGRSEELCETFKNYYTAQGMFGIPKKGEVEYTSDLELDLGDIQPSVAGPKRPQDRITVEALKSTWNEILVKPT-PGGYGKAPVVVGGEVANAPASLD-AKPEGNDVSRALGAETGVAPVPSDKPGYPYYEVTVDSKGGEKDAITHGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPSVKTSLGPGSRVVTDYLNKTGLQTELDKLGFQTVGYGCTTCIGNSGPLDAGIEDVVKAEDVVAASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDIDLSKDEI---IPGTGVYLKDIWPSLQEIQDALKSALSPEVFRALYTDFASQNPKWNEIPGSTGLVYDWNEKSTYIQHPPFFEDFSMEP-RDITEIVGARPLGIFGDSVTTDHISPAGAIKKTSPAGRFLSENNVTQADFNSYGSRRGNDRVMTRGTFANVRIKNLMLGGKEGGDTLL-----QPA----------GTEMSIYDAATAYMAAGTPSIIIGGEDYGMGSSRDWAAKGTRLLGVKAVITKSFERIHRSNLVGMGVLPCNFKDKADYDKVKDLSDATFSILGISNDTKPQSEATLRVTQADGSSFDIPLVVRIDTPVEIDYYRAGGILPYVLAQIL 929          

BLAST of aconitate hydratase vs. nr
Match: gi|1232408631|gb|OYW74531.1| (aconitate hydratase 1 [Verrucomicrobia bacterium 12-59-8])

HSP 1 Score: 1209.51 bits (3128), Expect = 0.000e+0
Identity = 620/950 (65.26%), Postives = 737/950 (77.58%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIP-EKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGAL--LLNSDDDPSNDVAIETQGS-KIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYF-----------GDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTT----SCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            M+T  TF  GS     ++SLPAL++ G+ +VSRLP+SIRIV+ESLLRNLDG+KVTE+DVKNLANWNAK+PG+YE+PFTVAR+VLQDFTGVPL+VDLAAMR      G +P +VEPLVPVDLVVDHSVQVDFAG+Q+AL++NL +EF RN+ERY+FLKWG+QAF+TF VVPPGIGIVHQVNLE+LA GVL KD V+YPD+LVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYL G L EGVTATDL L +T+ LR  GVVGKFVEFYGPGA +L + DRATIANMAPEYGATMGFF +D ETI YLRGTGR+EELC TV NY++AQ MWGIP EKG I++T ++E+DL++VVP VSGPKRPQDRI+VP L +KFN+L  A V  GG+G+ +ST   V  L  +   G    GA   +    D    +V ++++G  K  +  GSVLIAAITSCTNTSNPSVMLAAGL+AKKANA GLTV P VKTSLGPGSRVVTDYL  T LQ ELD+LGF+TVGYGCTTCIGNSGPL+  IE  +  +D+V ASVLSGNRNFEAR+H SI+ANFLMSPPLVVAYA+AG +DIDL+ + L     G+ +YLKD+WP+  E+++ + SAL P+VF+KLY++  + + KW  I+   G  ++W+G STYIQ PPFF +FS TP  +  ++   RPLG+FGDSVTTDHISPAGAI   SPAG++LLEHGV +A FNSFGSRRGND +MTRGTF NVRIKNLM  G EGG T +F           G+    A   ++    +     IY+AA  Y+      II+ GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNL+GMGVLP  FKDKADYDK+KD+   TF + GLS + K M E TL           +P++ R+DTP EIDYY +GGILPYVL QIL
Sbjct:    1 MNTPLTFTTGSGAPGKYYSLPALEAAGVGNVSRLPVSIRIVLESLLRNLDGKKVTEADVKNLANWNAKAPGEYEIPFTVARIVLQDFTGVPLLVDLAAMRSKVKELGKNPKMVEPLVPVDLVVDHSVQVDFAGTQAALNQNLELEFERNKERYQFLKWGEQAFDTFKVVPPGIGIVHQVNLEYLAKGVLEKDGVYYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLKGALKEGVTATDLVLEVTQKLRKLGVVGKFVEFYGPGAVSLPVTDRATIANMAPEYGATMGFFGVDEETIAYLRGTGRTEELCTTVTNYYKAQGMWGIPTEKGSIEFTTEMEMDLSTVVPCVSGPKRPQDRIEVPALKSKFNELLAADVKAGGFGK-SSTAQVVDHLGAAKADGLDVTGAAAGVDVVSDQTGFEVTVDSKGGIKDTITDGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPSVKTSLGPGSRVVTDYLTKTGLQVELDKLGFQTVGYGCTTCIGNSGPLDAGIEDVVKGQDVVAASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDIDLSTEEL---VAGSGVYLKDIWPTLKEVRDAMASALSPDVFRKLYTDFASQNPKWNEIQGGIGEVFEWDGKSTYIQHPPFFADFSMTPG-DITEITGARPLGIFGDSVTTDHISPAGAIKKASPAGRFLLEHGVTQADFNSFGSRRGNDLVMTRGTFANVRIKNLMLGGKEGGDTLHFERVPSGTSVLSGNTAATATKTELMKTSSGTEMSIYEAAERYKAENIPSIILGGEDYGMGSSRDWAAKGTRLLGVKAVITKSFERIHRSNLVGMGVLPCNFKDKADYDKVKDLSDATFDLLGLSNDFKPMSEATLRVHKADGSSFDVPVVVRIDTPVEIDYYRAGGILPYVLAQIL 945          

BLAST of aconitate hydratase vs. nr
Match: gi|910225250|ref|WP_050030911.1| (aconitate hydratase AcnA [Verrucomicrobium sp. BvORR034])

HSP 1 Score: 1202.58 bits (3110), Expect = 0.000e+0
Identity = 618/935 (66.10%), Postives = 718/935 (76.79%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTS----CGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            +D LRTF+ GS     F+SLP L+  G+  VS+LP+SIR+V+ESL+RNLDG+KVT  DV+NLANWNAK+PGDYE+PFTVAR+VLQDFTGVPL+VDLAAMR A    G +P ++EPLVPVDLVVDHSVQVDFAG+Q +L+RNL +EF RN+ERY+FLKWG+QAF+TF VVPPGIGIVHQVNLE+LA GVL K+ VFYPD+LVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVG+ L G L  GVTATDL LR+TE+LR   VVGKFVEF+GPGA ALS+PDRATIANMAPEYGATMGFF +D  TI YL GTGRS ELC TVENY++AQ +WGI E  EI+YT  +ELD+++V P V+GPKRPQDRIDV  L  KF  L  A                       TP GF   G   LN   + + D      G K ++ HGSVLIAAITSCTNTSNPSVMLAAGL+AKKANA GLTV P VKTSLGPGSRVVTDYL  T LQ ELDQLGF+TVGYGCTTCIGNSGPL+P IE  + S+D+V ASVLSGNRNFEAR+H SI+ANFLMSPPLVVAYA+AG +D+DL+ +PLG   +G  + L D+WPS  E+   + SAL P+VFQKLY+     + KW  I S+ G  YDW+ DSTYIQ PPFFD FS  P ++  ++   RPLG+F DSVTTDHISPAG I   SPAGKYLLE+ V +A FNS+GSRRGNDR+MTRGTF NVRIKNLM PGIEGG T+YF    V        SAE A+   IYDAA  Y+ +G  L+I+ GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNL+GMGVLP  F +K DYDK+K +   TFS+ G+S ++K M E TL  +       ++P+I RLDTP EIDYY +GGILPYVL QIL
Sbjct:    3 LDALRTFKTGSNTDGKFYSLPELEKQGVGPVSKLPVSIRVVLESLVRNLDGKKVTLKDVQNLANWNAKAPGDYEIPFTVARIVLQDFTGVPLLVDLAAMRSAVHAMGKNPKMIEPLVPVDLVVDHSVQVDFAGTQDSLNRNLDLEFHRNRERYKFLKWGEQAFDTFKVVPPGIGIVHQVNLEYLAKGVLEKENVFYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGMRLTGALQPGVTATDLVLRVTEVLRKTKVVGKFVEFFGPGARALSVPDRATIANMAPEYGATMGFFGIDEATIAYLTGTGRSAELCGTVENYYKAQGLWGIQEGDEIEYTQVVELDMSTVKPGVAGPKRPQDRIDVSALKEKFESLLVA----------------------PTPDGF---GKPSLNGQYEVTVD---SKDGVKDSITHGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPSVKTSLGPGSRVVTDYLEKTGLQTELDQLGFQTVGYGCTTCIGNSGPLDPGIENVVRSEDVVAASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDVDLSKEPLGTGSDGQPVLLADIWPSSEEIGAAMASALAPDVFQKLYTGFAEQNPKWNEIPSSVGLVYDWDRDSTYIQEPPFFDKFSMEP-SDINEIHGARPLGIFSDSVTTDHISPAGNIKKDSPAGKYLLENQVTQADFNSYGSRRGNDRVMTRGTFANVRIKNLMVPGIEGGITKYFSGPGV--------SAEAAEVLPIYDAAEQYKAAGVPLVILGGEDYGMGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLVGMGVLPCNFVNKEDYDKVKGLTDATFSLLGVSNDIKPMSEATLKVTQADGVTFNVPVIVRLDTPVEIDYYRAGGILPYVLAQIL 900          

BLAST of aconitate hydratase vs. nr
Match: gi|740322634|ref|WP_038159764.1| (aconitate hydratase AcnA [Verrucomicrobium sp. BvORR106])

HSP 1 Score: 1201.42 bits (3107), Expect = 0.000e+0
Identity = 618/935 (66.10%), Postives = 718/935 (76.79%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTS----CGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            +D LRTF+ GS     F+SLP L+  G+  VS+LP+SIR+V+ESL+RNLDG+KVT  DV+NLANWNAK+PGDYE+PFTVAR+VLQDFTGVPL+VDLAAMR A    G +P ++EPLVPVDLVVDHSVQVDFAG+Q +L+RNL +EF RN+ERY+FLKWG+QAF+TF VVPPGIGIVHQVNLE+LA GVL K+ VFYPD+LVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVG+ L G L  GVTATDL LR+TE+LR   VVGKFVEF+GPGA ALS+PDRATIANMAPEYGATMGFF +D  TI YL GTGRS ELC TVENY++AQ +WGI E  EI+YT  +ELD++SV P V+GPKRPQDRIDV  L  KF  L  A                       TP GF   G   LN   + + D      G K +++HGSVLIAAITSCTNTSNPSVMLAAGL+AKKANA GLTV P VKTSLGPGSRVVTDYL  T LQ ELDQLGF+TVGYGCTTCIGNSGPL+P IE  + ++D+V ASVLSGNRNFEAR+H SI+ANFLMSPPLVVAYA+AG +D+DL+ +PLG   +G  + L D+WPS  E+   + SAL PEVFQKLY+     + KW  I S+ G  YDW+ DSTYIQ PPFFD FS  P ++  ++   RPLG+F DSVTTDHISPAG I   SPAGKYLLE+ V +A FNS+GSRRGNDR+MTRGTF NVRIKNLM PG EGG T+YF    V        SAE A+   IYDAA  Y+ +G  L+I+ GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNL+GMGVLP  F +K DYDK+K +   TFS+ G+S ++K M E TL  +       ++P+I RLDTP EIDYY +GGILPYVL QIL
Sbjct:    3 LDALRTFKTGSNTDGKFYSLPELEKQGVGPVSKLPVSIRVVLESLVRNLDGKKVTLKDVQNLANWNAKAPGDYEIPFTVARIVLQDFTGVPLLVDLAAMRSAVHAMGKNPKMIEPLVPVDLVVDHSVQVDFAGTQDSLNRNLDLEFHRNRERYKFLKWGEQAFDTFKVVPPGIGIVHQVNLEYLAKGVLEKENVFYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGMRLTGALQPGVTATDLVLRVTELLRKTKVVGKFVEFFGPGARALSVPDRATIANMAPEYGATMGFFGIDEATIAYLTGTGRSAELCGTVENYYKAQGLWGIQEGDEIEYTQVVELDMSSVKPGVAGPKRPQDRIDVAALKEKFESLLVA----------------------PTPDGF---GKPSLNGQYEVTVD---SKDGVKDSIKHGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPSVKTSLGPGSRVVTDYLEKTGLQTELDQLGFQTVGYGCTTCIGNSGPLDPGIENVVRNEDVVAASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDVDLSKEPLGTGSDGQPVLLADIWPSSEEIGAAMASALAPEVFQKLYTGFAEQNPKWNEIPSSVGLVYDWDRDSTYIQEPPFFDKFSMEP-SDINEIHGARPLGIFSDSVTTDHISPAGNIKKDSPAGKYLLENQVTQADFNSYGSRRGNDRVMTRGTFANVRIKNLMVPGTEGGITKYFSGPGV--------SAEAAEVLPIYDAAEQYKAAGVPLVILGGEDYGMGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLVGMGVLPCNFVNKEDYDKVKGLTDATFSLLGVSNDIKPMSEATLKVTQADGVTFNVPVIVRLDTPVEIDYYRAGGILPYVLAQIL 900          

BLAST of aconitate hydratase vs. nr
Match: gi|497651315|ref|WP_009965499.1| (aconitate hydratase AcnA [Verrucomicrobium spinosum])

HSP 1 Score: 1199.88 bits (3103), Expect = 0.000e+0
Identity = 616/935 (65.88%), Postives = 717/935 (76.68%), Query Frame = 0

Query:    1 MDTLRTFENGS-----FHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTTSCG----LSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            +D LRTF+ GS     F+SLP L+  G+  VS+LP+SIR+V+ESL+RNLDG+KVT  DV+NLANWNAK+PGDYE+PFTVAR+VLQDFTGVPL+VDLAAMR A    G +P ++EPLVPVDLVVDHSVQVDFAG+Q +L+RNL +EF RN+ERY+FLKWG+QAF+TF VVPPGIGIVHQVNLE+LA GVL K+ VFYPD+LVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVG+ L G L  GVTATDL LR+TE+LR   VVGKFVEF+GPGA ALS+PDRATIANMAPEYGATMGFF +D  TI YL GTGRS ELC TVENY++AQ +WGI E  EI+YT  +ELD+++V P V+GPKRPQDRIDV  L  KF  L  A                       TP GF   G   LN   + + D      G K ++ HGSVLIAAITSCTNTSNPSVMLAAGL+AKKANA GLTV P VKTSLGPGSRVVTDYL  T LQ ELDQLGF+TVGYGCTTCIGNSGPL+P IE  + S+D+V ASVLSGNRNFEAR+H SI+ANFLMSPPLVVAYA+AG +D+DL+ +PLG   +G  + L D+WPS  E+   + SAL P+VFQKLY+     + KW  I S+ G  YDW+ DSTYIQ PPFF+ FS  P ++  ++   RPLG+F DSVTTDHISPAG I   SPAGKYLLE+ V +A FNS+GSRRGNDR+MTRGTF NVRIKNLM PG EGG T+YF    V        SAE A+   IYDAA  Y+ +G  L+I+ GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNL+GMGVLP  F +K DYDK+K +   TFS+ G+S ++K M E TL  +       ++P+I RLDTP EIDYY +GGILPYVL QIL
Sbjct:    3 LDALRTFKTGSNTDGKFYSLPELEKQGVGPVSKLPVSIRVVLESLVRNLDGKKVTLKDVQNLANWNAKAPGDYEIPFTVARIVLQDFTGVPLLVDLAAMRSAVHAMGKNPKMIEPLVPVDLVVDHSVQVDFAGTQDSLNRNLDLEFHRNRERYKFLKWGEQAFDTFKVVPPGIGIVHQVNLEYLAKGVLEKENVFYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGMRLTGALQPGVTATDLVLRVTEVLRKTKVVGKFVEFFGPGARALSVPDRATIANMAPEYGATMGFFGIDEATITYLAGTGRSAELCGTVENYYKAQGLWGIQEGDEIEYTQVVELDMSTVKPGVAGPKRPQDRIDVSALKEKFESLLVA----------------------PTPDGF---GKASLNGQYEVTVD---SKDGVKDSITHGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPSVKTSLGPGSRVVTDYLEKTGLQTELDQLGFQTVGYGCTTCIGNSGPLDPGIENVVRSEDVVAASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDVDLSKEPLGTGSDGQPVLLADIWPSSEEIGAAMASALAPDVFQKLYTGFAEQNPKWNEIPSSVGLVYDWDRDSTYIQEPPFFEKFSMDP-SDINEIHGARPLGIFSDSVTTDHISPAGNIKKDSPAGKYLLENQVTQADFNSYGSRRGNDRVMTRGTFANVRIKNLMVPGTEGGITKYFSGPGV--------SAEAAEVLPIYDAAEQYKAAGVPLVILGGEDYGMGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLVGMGVLPCNFVNKEDYDKVKGLTDATFSLLGVSNDIKPMSEATLKVTQADGETFNVPVIVRLDTPVEIDYYRAGGILPYVLAQIL 900          

BLAST of aconitate hydratase vs. nr
Match: gi|1332714519|ref|WP_102725714.1| (aconitate hydratase AcnA [Akkermansia muciniphila] >gi|1329749278|gb|PNC05154.1| aconitate hydratase AcnA [Akkermansia muciniphila])

HSP 1 Score: 1185.63 bits (3066), Expect = 0.000e+0
Identity = 597/921 (64.82%), Postives = 702/921 (76.22%), Query Frame = 0

Query:   10 GSFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTT----SCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            G F+SLPAL   G + ++RLP+SIRIV+ESLLRN DG KVTE DV NLA+WNAK+PG YE+PFTVAR+VLQD TGVPL+VDLAAMR A A  G D +++EPLVPVDLVVDHSVQVD+AG   AL++NL IEF RN ERYEFLKWGQQAF TF VVPP +GIVHQVNLE+LA GV+ KD V++PDTLVGTDSHTTMING+GVVGWGVGGIEAEAGMLGQPVTFLVPEVVGV++ G+L EGVTATDL L +T++LR HGVVGKFVEF+G GA AL L DRAT+ANMAPEYGATMGFFP+D     YLR TGRSEE   T ENYF+AQ +WG+P  GE+DYTD LELDL+++ PAVSGPKRPQD I +  +   F  L +    EGGYG++ S    VA   + +PAG                  V +E    +  L+ GS+LIAAITSCTNTSNP +MLAAGL+AKKA ALGL V P VKTS+ PGSR+ +DY  A  LQ  LD LGFETVGYGCTTCIGNSGPLNPE+E A+   D+V ASVLSGNRNFEARIH SI+ NFLMSPPLVVA+ALAGR+DID   +PLG D++GN ++LKD+WPS AE+ E +  +  PE +++LY+     + +W  + +  G+ + WN +STYIQ PPFF  F+  P T   D+ D RPLG+FGDSVTTDHISPAGAI  T PAG YL   G+++  FNSFGSRRGNDR+MTRGTF NVRIKNLM  G EGGYT YFG+  +PAPD+ I +   A A FIYDAA+AY Q G  LI+I GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNLIGMGVLPL F DKADYD++  +   TFSI GL G +   Q+VTL      +    +P+I R+DTP E +YYL+GGIL YVL QIL
Sbjct:   17 GQFYSLPALAENGYSRLNRLPVSIRIVLESLLRNCDGLKVTEKDVDNLASWNAKNPGSYEIPFTVARIVLQDLTGVPLLVDLAAMRSAVAGLGKDASVIEPLVPVDLVVDHSVQVDWAGRTDALEKNLDIEFQRNAERYEFLKWGQQAFHTFSVVPPSVGIVHQVNLEYLAQGVMEKDGVYFPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVHMTGELREGVTATDLALHVTQMLRNHGVVGKFVEFFGEGAAALPLADRATVANMAPEYGATMGFFPMDEHCSEYLRQTGRSEEAITTYENYFKAQGLWGMPRNGELDYTDQLELDLSAIDPAVSGPKRPQDHIPLTSIRDSFRKLVSMPAAEGGYGKKESPSVKVA---MHSPAGVP----------------VPVEGFSQEAELKDGSILIAAITSCTNTSNPGLMLAAGLLAKKAVALGLQVPPHVKTSIAPGSRIASDYFAANQLQDSLDALGFETVGYGCTTCIGNSGPLNPELEQAVRDNDIVAASVLSGNRNFEARIHASIKTNFLMSPPLVVAFALAGRVDIDFQTEPLGTDRSGNPVFLKDIWPSAAEIAEAVAKSRNPEAYRELYTITPEKNPRWAAVYAPEGSVFQWNENSTYIQNPPFFCGFNSQPRTLA-DIRDARPLGIFGDSVTTDHISPAGAIRETGPAGLYLQGMGIDRKDFNSFGSRRGNDRVMTRGTFANVRIKNLMVDGTEGGYTLYFGNRAIPAPDKKIAATAGAPA-FIYDAAMAYAQDGVPLIVIGGEDYGMGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLIGMGVLPLNFADKADYDRVATLKDATFSILGLEGGISPRQQVTLRVQPAGAEAFDVPVIVRIDTPIEKEYYLAGGILQYVLTQIL 916          

BLAST of aconitate hydratase vs. nr
Match: gi|1332698902|ref|WP_102714919.1| (aconitate hydratase AcnA [Akkermansia muciniphila] >gi|1329761461|gb|PNC16890.1| aconitate hydratase AcnA [Akkermansia muciniphila])

HSP 1 Score: 1181.01 bits (3054), Expect = 0.000e+0
Identity = 595/921 (64.60%), Postives = 701/921 (76.11%), Query Frame = 0

Query:   10 GSFHSLPALKSLGIADVSRLPISIRIVIESLLRNLDGRKVTESDVKNLANWNAKSPGDYEVPFTVARVVLQDFTGVPLVVDLAAMRDAAANAGADPAIVEPLVPVDLVVDHSVQVDFAGSQSALDRNLSIEFIRNQERYEFLKWGQQAFETFGVVPPGIGIVHQVNLEHLATGVLSKDEVFYPDTLVGTDSHTTMINGLGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVYLHGKLAEGVTATDLTLRITEILRAHGVVGKFVEFYGPGAEALSLPDRATIANMAPEYGATMGFFPLDSETINYLRGTGRSEELCATVENYFRAQEMWGIPEKGEIDYTDDLELDLASVVPAVSGPKRPQDRIDVPDLGAKFNDLFTASVDEGGYGREASTRNDVAELDLSTPAGFSPEGALLLNSDDDPSNDVAIETQGSKIALQHGSVLIAAITSCTNTSNPSVMLAAGLVAKKANALGLTVAPFVKTSLGPGSRVVTDYLNATHLQKELDQLGFETVGYGCTTCIGNSGPLNPEIEAALTSKDLVCASVLSGNRNFEARIHGSIRANFLMSPPLVVAYALAGRIDIDLTNDPLGQDQNGNDIYLKDLWPSPAELQEVIGSALKPEVFQKLYSNLDTASEKWVGIESATGATYDWNGDSTYIQLPPFFDNFSRTPATEGQDLIDTRPLGVFGDSVTTDHISPAGAITATSPAGKYLLEHGVEKAKFNSFGSRRGNDRIMTRGTFGNVRIKNLMTPGIEGGYTQYFGDAHVPAPDQDIPSAENAKATFIYDAAVAYQQSGRKLIIIAGEDYGMGSSRDWAAKGTALLGVKAVITKSFERIHRSNLIGMGVLPLTFKDKADYDKIKDMHSCTFSITGLSGELKTMQEVTLTT----SCGLSLPLIARLDTPAEIDYYLSGGILPYVLNQIL 926
            G F+SLPAL   G + ++RLP+SIRIV+ESLLRN DG KVTE DV NLA+WNAK+PG YE+PFTVAR+VLQD TGVPL+VDLAAMR A A  G D +++EPLVPVDLVVDHSVQVD+AG   AL++NL IEF RN ERYEFLKWGQQAF TF VVPP +GIVHQVNLE+LA GV+ KD V++PDTLVGTDSHTTMING+GVVGWGVGGIEAEAGMLGQPVTFLVPEVVGV++ G+L EGVTATDL L +T++LR HGVVGKFVEF+G GA AL L DRAT+ANMAPEYGATMGFFP+D     YLR TGRSEE   T ENYF+AQ +WG+P  GE+DYTD LELDL+++  AVSGPKRPQD I +  +   F  L +    EGGYG++ S    VA   + +PAG                  V +E    +  L+ GS+LIAAITSCTNTSNP +MLAAGL+AKKA ALGL V P VKTS+ PGSR+ +DY  A  LQ  LD LGFETVGYGCTTCIGNSGPLNPE+E A+   D+V ASVLSGNRNFEARIH SI+ NFLMSPPLVVA+ALAGR+DID   +PLG D++GN ++LKD+WPS AE+ E +  +  P+ +++LY+     + +W  + +  G+ + WN +STYIQ PPFF  F+  P T   D+ D RPLG+FGDSVTTDHISPAGAI  T PAG YL   G+++  FNSFGSRRGNDR+MTRGTF NVRIKNLM  G EGGYT YFG+  +PAPD+ I +   A A FIYDAA+AY Q G  LI+I GEDYGMGSSRDWAAKGT LLGVKAVITKSFERIHRSNLIGMGVLPL F DKADYD++  +   TFSI GL G +   Q+VTL      +    +P+I R+DTP E +YYL+GGIL YVL QIL
Sbjct:   17 GQFYSLPALAENGYSRLNRLPVSIRIVLESLLRNCDGLKVTEKDVDNLASWNAKNPGSYEIPFTVARIVLQDLTGVPLLVDLAAMRSAVAGLGKDASVIEPLVPVDLVVDHSVQVDWAGRTDALEKNLDIEFQRNAERYEFLKWGQQAFHTFSVVPPSVGIVHQVNLEYLAQGVMEKDGVYFPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVTFLVPEVVGVHMTGELREGVTATDLALHVTQMLRNHGVVGKFVEFFGEGAAALPLADRATVANMAPEYGATMGFFPMDEHCSEYLRQTGRSEEAITTYENYFKAQGLWGMPRNGELDYTDQLELDLSAIDSAVSGPKRPQDHIPLTSIRDSFRKLVSMPAAEGGYGKKESPSVKVA---MHSPAGVP----------------VPVEGFSQEAELKDGSILIAAITSCTNTSNPGLMLAAGLLAKKAVALGLQVPPHVKTSIAPGSRIASDYFAANQLQDSLDALGFETVGYGCTTCIGNSGPLNPELEQAVRDNDIVAASVLSGNRNFEARIHASIKTNFLMSPPLVVAFALAGRVDIDFQTEPLGTDRSGNPVFLKDIWPSAAEIAEAVAKSRNPKAYRELYTITPEKNPRWAAVYAPEGSVFQWNENSTYIQNPPFFCGFNSQPRTLA-DIRDARPLGIFGDSVTTDHISPAGAIRETGPAGLYLQGMGIDRKDFNSFGSRRGNDRVMTRGTFANVRIKNLMVDGTEGGYTLYFGNRAIPAPDKKIAATAGAPA-FIYDAAMAYAQDGVPLIVIGGEDYGMGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLIGMGVLPLNFADKADYDRVATLKDATFSILGLEGGISPRQQVTLRVQPAGAEAFDVPVIVRIDTPIEKEYYLAGGILQYVLTQIL 916