H F60

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General

End Date: 

Monday, February 25, 2013 - 00:00

Experiment Batch ID: 

H

Start Date: 

Tuesday, January 15, 2013 - 13:17

Experiment Contact: 

Sussie Dalvin
Sample

Developmental Stage CV: 

PreAdult2
Adult female

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

FK506

Primary target ID: 

EMLSAG00000003343, EMLSAG00000003343-686109 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F60

Fragment concentration: 

600.00ng/ul

Fragment length: 

542bp

Sense sequence: 

ccacgggcccaggaactgttcatttgacaggttatttgattcccgacactgacgacatgattgacgacctcgattcagagtctgaatgtttnnnnnnnnnnnnnnnnnnagctcctgtccttgttaatggaaagggcaagaacaccaaacgtaagcctgataatagcttaattgatactcctaataaaaaagctaaaaaagagttggaacttgctagagctaaaaaagttcagtccgatcttaacttgaagaagaagagtcaaaagaaagtcgagtcttcggatgatgataacgatatggaagcagatgatgatacgtcggatgatggaatattagatttcactgagaattacgctgaagaagaggactctgtcgatgaatctgatgacgaatacgaggatgattcttcagtagaagaaggcgattcctctccaacaaagaaaattctcgagaaaatagccaataaaaaggagtcttctctcactactcccaagaacaataagaaaaaggataatgcctccactcctaaaggcgctactccc

Primer Group: 

FK506_f_b1313
Validation

Efficacy of Knock Down: 

F>14.00%
Phenotype

Phenotype CV: 

Phenotype: 

no reproduction

Number of Individuals (End): 

13

Gallery Image: 

Image file: 

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Females looked normal, but none had produced any eggstrings. Some animals had small empty eggstrings hanging behind them. Some eggs appeared vitellogenised but thin.  Digestion looked normal.

 

Little pathology on RNAi animals sections. Eggs not vitellogenized

Uncertain about number of animals at endpoint