D F26

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General

End Date: 

Wednesday, October 3, 2012 - 00:00

Experiment Batch ID: 

d

Start Date: 

Friday, August 24, 2012 - 13:17

Experiment Contact: 

Frank Nilsen
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

g10296_cath

Primary target ID: 

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F26

Fragment concentration: 

600.00ng/ul

Fragment length: 

459bp

Sense sequence: 

tggacaagttgccggccacttttcaagtgataccgcctgcattgctgatgtatgcccaacagaacaagcctttggagaggtattgaagcaacctggaattcaattcattgcggccaagtttgatggtatccttggtatgggattcaaaaggatttccttggaaggtgtccctccagtgttccaaagtatggttgaccaaaagttggtagatgatgctgtattctccttttggttgaacagagacgttgatgaagaatttggaggacgtcttactttgggaggaactgactcttctctctatgaaggagatattacttacattgaccttgttggagaggactactggagaatcaagatggactccatgaaaatgggaaatgaaagtattggatgtgcaggaactgaagaaaaccctggatgtatggccatcgttgataccggatgctccctctctgttgg

Primer Group: 

g10296_cath_f_b799
Validation

Efficacy of Knock Down: 

F>80.00%
Phenotype

Phenotype: 

No visible phenotype

Number of Individuals (End): 

7
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Cath, elevated mortality, but looks normal otherwise Cath: Significantly downregulated (with primers outside and inside fragment)