D F31

Submitted by on

General

End Date: 

Wednesday, October 3, 2012 - 00:00

Experiment Batch ID: 

d

Start Date: 

Friday, August 24, 2012 - 13:31

Experiment Contact: 

Sussie Dalvin

Publication: 

Molecular characterization and functional analysis of components of the TOR pathway of the salmon louse, Lepeophtheirus salmonis (Krøyer, 1838)
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

TOR

Primary target ID: 

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F31

Fragment concentration: 

600.00ng/ul

Fragment length: 

505bp

Sense sequence: 

tgtctggggactcagcctccaagatgggaggtatcctggccatagttgctctactcaacgcggatgtttgtaacacggggagtcgaattcatcgatttggaaattatcttcaaaacaattgtttaccgggagggaactccgtgacggatcctgtggtgattgccctggctactaaagccattggccgacttactcaagtatatggtagctacaaggctaatttacgctccgacttcattaattcactcgtcaagaaatcatttgagtggctttccaaccccaatgatcgcaatgatctcaagtggtacgctgctgttctcattctaagagaaatagccatttgtgagcccactttctttttccagaacgtaccaacgttttttgatcttatcttcattcctgtcagagactcaaaaccatttcttagagaatcagccgttaatgcgctacgagctggtctcatcgttactgcccaaagagaaaccaccaaacaatccagacgcca

Primer Group: 

LsTOR_f_b807
Validation

Efficacy of Knock Down: 

F>0.10%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

14

Gallery Image: 

Image file: 

File RD38.tiff
Upload

Image Quality: 

0
No votes yet

TOR, short eggstrings (14763), stunted growth of genital segment (90% of control), 2 ødipus eggstrings TOR: Significant downregulation (but only with primers outside the dsRNA fragments, both tested) NB! No downregulation BUT upregulation -95%