D F32

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General

End Date: 

Wednesday, October 3, 2012 - 00:00

Experiment Batch ID: 

d

Start Date: 

Friday, August 24, 2012 - 13:42

Experiment Contact: 

Sussie Dalvin

Publication: 

Molecular characterization and functional analysis of components of the TOR pathway of the salmon louse, Lepeophtheirus salmonis (Krøyer, 1838)
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

NLG3408_TSC1

Primary target ID: 

EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F32

Fragment concentration: 

600.00ng/ul

Fragment length: 

549bp

Sense sequence: 

ctgctctgagagatcaactccagcttgtcaaatcagaaattgatgatttacaccgtcaaattgaggaaataagaagggaaaaacatctgatagaggaagaaaggtcatttactgcaagaaatagaggtcatgaaatcgaaaaaaggtctgctgagattagatctttaaaaatcagtgcacaaaaatctgaagaaataattgatgagctcaaccatgctgtagtagaaggaaggaaggagttggatacctccagggctcaatttttcctaatgaaagctgaatgcgaggttttacatcaaaaagaaagcgcaagaatgatgacggaattggaattggttcgactgagaaaggaacttgtcatgatgggtgagcttcagtcaaaatataaggagcggttggataatcctgctaacaacatggaatctgagaccatgaatctctttaagcaggcttatgaaagggatctacagactatggccagagctctggagaacaaaacatttgaagtagagacattcaagtgtcgtatcaccgagttggactccttgc
Validation

Efficacy of Knock Down: 

F>33.00%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

7

Gallery Image: 

Image file: 

File RD83.tiff
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Tsc1, high mortality but looks normal TSC-1: Not significantly downregulated but only checked with primers inside ds fragment