D F33

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General

End Date: 

Wednesday, October 3, 2012 - 00:00

Experiment Batch ID: 

d

Start Date: 

Friday, August 24, 2012 - 13:49

Experiment Contact: 

Sussie Dalvin

Publication: 

Molecular characterization and functional analysis of components of the TOR pathway of the salmon louse, Lepeophtheirus salmonis (Krøyer, 1838)
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

g18363_TSC2

Primary target ID: 

EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F33

Fragment concentration: 

600.00ng/ul

Fragment length: 

432bp

Sense sequence: 

aaaatgggcattcagcgacttggttgattggcacaatgcttataactgtttctacgagctgttgtacccatattgcaatgagaaatggtgtatgtgatagatgttatttttattgcattcgagatggaactttaagtaaggatgatattactaaaaattcggatagaagaagacacatgtcggatttttactccggtacaaataagagtcagttatcaaacttaagaagtgataaggaacttcataggcacacttctctggagagaatggagtcttctggagatgattatagagtaaaaaaagtgctacctgaaacatgcagttgttggtgctctggctgggctgaaatttacattcggcgtccaagtggggatgtatcctggatgtgtagagtgcaaaatactcagttgcttagtgcgcatacaccctcaa

Fragment Producer: 

licelab

Primer Group: 

LS_TSC2-F_b872
Validation

Efficacy of Knock Down: 

F>72.00%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

11

Gallery Image: 

Image file: 

File RD88.tiff
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Tsc2, a bit short eggstring TSC-2: Significant downregulation (but only with primers outside the dsRNA fragments, both tested)