H F24

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General

End Date: 

Monday, February 25, 2013 - 00:00

Experiment Batch ID: 

F24

Start Date: 

Tuesday, January 15, 2013 - 15:07

Experiment Contact: 

Frank Nilsen
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

g3536_Tryp3

Primary target ID: 

EMLSAT00000002836, EMLSAT00000002836-698683 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F24

Fragment concentration: 

600.00ng/ul

Fragment length: 

633bp

Sense sequence: 

gctgttgccgccaatgctggttctataatgaaacatagtggaagaattgtcggaggagatgaagttgagcccaactctattccattccaaatttcaattgaaatcagtggaggctaccacttttgtggtggttctgtcatggacaaggatacaattatcactgctggtcattgctgtactaacttgaaacccgaagacattctagtcgttgctggtgaacacgatttcctctctatgagtggagatgagcaaaagattgctgtatctgaaatgacccgccacgataagtttggaacatatggtcctaactatgacatttgtcttttgaaattgaagtcttccttggccctcaacgagaaagtcaagcctgttgctctcccagaaaaggaccaagaattcaccggtgatgttgttgtgtccggttggggaactgtcgaattcggtggctcatcttctgatgttcttagatcagttaccctacaacttgtttctgatgagtattgtagtgatgcctacgatggacatactgatgaaagcatgatctgtgctgggtcacccggaaaagactcatgccaaggtgactccggtggcccattgact

Fragment Producer: 

licelab

Primer Group: 

g3536_tryp3F_b830
Validation

Efficacy of Knock Down: 

F>89.00%
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Opsummering:
F24, phenotype ?, QPCR confirms down regulation
F24 28,6
Kontroll 23,9

Mohammed laget dsRNA fragment