BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000004991 (supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39")

HSP 1 Score: 952.584 bits (2461), Expect = 0.000e+0
Identity = 451/543 (83.06%), Postives = 488/543 (89.87%), Query Frame = 0

Query:  868 DQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGF 1410
            D  E ++ S SE   +    +ESLSE+L L  LW+AL  CLKDLA+TPDHHAVLVLQ++VEAFFLVHAA TQP+EKKK  +KETRQEQLAHIQEQL   K  + ++   +S   S SL+ DT KFL FA+THRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDE +RREDLAVHVRRENVFE+SFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIY+ SQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL KPVKYTDMESEDY+FYKGLEFL+ H VSDLGY+LTFSTEI+EFGVTE+RDLIPNGRNI V+E+TK++Y+RLVCQMKMT AIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLP +D+DDLK+N+EYHKYQ NSLQI+WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGF+ LEGMNG QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLR+YLLKAI ECSE   F
Sbjct: 2417 DVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSST--ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957          

HSP 2 Score: 339.347 bits (869), Expect = 3.118e-95
Identity = 205/357 (57.42%), Postives = 249/357 (69.75%), Query Frame = 0

Query:   76 GAIGSSASETGAPST-----EQDYATILGMDISELPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAA-HVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSL-----HQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASV--SFETPPAKIRKGATKTSGTLVETSTPTSVASS 419
            G     AS    PST       DY++ILG D+ E+PEGVDPSFLAALPE+MRQEVID+QRRLQ++RQRA   + E + V EVNPEFLAALPPNIQEEVL QQRMEQQRQ +   NPEAPVDPGEF QTLP SLRQSVLADME+SQM+ALPA+LAAEAQ+LRRE E RNR  + ER FN+   GGNS L+SILR + +  G+QY+IG   N  GR   R   +   +   +++     HQA+   +++ KGR LLD+EGLSCLLILLF++D+KINT RLHRILRNLCYH PTRDWV+  L+SILE++NE K +       +AS+  SF+ P    R            T+TP   ASS
Sbjct: 1867 GGXSXIASSNPIPSTSGTSQNPDYSSILG-DV-EIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNS-LSSILRGSVSRLGNQYIIG---NLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDI-------MASIQSSFQLPNKSKRSS----------TATPVKSASS 2196          

HSP 3 Score: 141.739 bits (356), Expect = 7.668e-34
Identity = 95/172 (55.23%), Postives = 117/172 (68.02%), Query Frame = 0

Query:  599 FSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSS-NQAQEAIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTK 769
            + VIRR+S+LTDKLLRLLSLIS+G+PD    + DK+     E S S N     I  D L+LAV+VLTSK+CSEEGLEDV ALLLNLS+GP PTRD IL+LLL+GAQELGNVVR NVL LQ EL  +K+   +   S                KG  +DRFT+++V+L AP+K
Sbjct: 2204 YPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRS-------------GKDKGGLLDRFTKETVVLNAPSK 2362          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000011039 (supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41")

HSP 1 Score: 306.605 bits (784), Expect = 2.606e-88
Identity = 170/477 (35.64%), Postives = 256/477 (53.67%), Query Frame = 0

Query:  962 QLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTH-RTVLNQILRQSTTHL---ADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVR----RENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGL--------------------LREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGF 1410
            Q E P+++      P  L P   +         F D + RT   Q  R   T+    A G + V V + R          FR +L +     +   L  H++    R+ +FEDSF +L R    E + R YI+F GEEG D GG                     LREW+ ++S E+ NPMY LF+ +  +  +  I   S  NP+HL YFKF+GR IA A+Y  K +   FT  FYK +L K +   D+ES D  +Y  L ++ ++ + + G E+ F  +    G  +  +L P G  + V+E+ K EY+ +VC+ +M+  I +Q  +FLEGF  ++P   +  F+E+ELEL+L G+  +DVDD + +S Y  Y  NS QI WFW+ +RS D   +++ +QFV G+ +VP+ GF+ L G NG Q+F + +  + +  LP +HTCFN+LDLP Y++YD+L   L  AI+E +EGFG 
Sbjct:  411 QWEDPRTQGQVKEDP--LPPGWEIRFMAGGVPYFVDHNTRTTTFQDPRPGATNAKGGAKGAYGVPVQYERS---------FRWKLSQFRYLCQSNSLPSHIKLSVTRQTLFEDSFHQLMRLPAFELRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQRFCVEKVGKDS-WLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEE-TEGFGL 874          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000007514 (supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13")

HSP 1 Score: 299.671 bits (766), Expect = 2.778e-86
Identity = 160/385 (41.56%), Postives = 231/385 (60.00%), Query Frame = 0

Query: 1032 DIKRRY--FRTELERMDENMRREDLAVHVRRENVFEDSFRELHRR----SQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQ-TNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYH-KYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGF 1408
            D KR+Y   +++L++      +    + +RR  + EDSF+++  +      +  K + +I F+GE G D GG+ REW+ ++S+E+FNP Y LF+ S  D  T  I   S   N +HL YFKF+GR    A    KLL+ +F R FYK +L K ++ +DMES D   +  L ++ E+   +L  ELTF  +   FG T  R+L P G+NI V+ + K EY+  V Q +  S I+KQ+  FL GF  ++P   I IF+E ELELL+ G+ ++DV D K N+ Y   Y  N + I W+WR + SF+   +++ +QFVTG+S+VP+ GF  L G NGPQ F I R    +   P AHTCFN+LDLP YE+Y +LR  L+KAI E SEGF
Sbjct:  370 DYKRKYEFLKSQLKKPSNVPNK--FEIKIRRRYILEDSFQKIFMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQEL--ELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIERWGNPSS-YPRAHTCFNRLDLPPYESYQQLRDRLIKAI-EGSEGF 748          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000006171 (supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36")

HSP 1 Score: 285.419 bits (729), Expect = 1.287e-80
Identity = 138/357 (38.66%), Postives = 216/357 (60.50%), Query Frame = 0

Query: 1058 VRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALE---GMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            V R+ VFEDS+R++ +   +  + +  + F  EEG D GG+ REW  ++S E+ NP Y LF+ S  D  T  I   S  NP+HL YF FVGR+I  A++    ++  FT  FYK +L KP+   D+E+ D   +K L++++ + +S +  + TFS E   FG+T+V +L  NG++I V+E  K+EYV+L  Q +    I +Q  +  +GF  +IP+ L+  F+E+ELEL++ G+  +D+ D K ++   +  A +  + WFW  + S+    +A+ +QFVTGSS+VPLQGF  L+   G  GP+ F +H  +   D LP A TCFN+LDLPAY +++ +   L +A++E     GFA
Sbjct:  578 VSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETC---GFA 930          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000003493 (supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16")

HSP 1 Score: 162.54 bits (410), Expect = 1.316e-40
Identity = 115/375 (30.67%), Postives = 187/375 (49.87%), Query Frame = 0

Query: 1054 LAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEG-----EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKV-----TYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAK---PVKYTDMESEDYSFYKGLEFLIEH--TVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPN-VDVDDLKANSEYH-KYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            + V +RR+ ++ED+  +L   ++   K +  + F+      E G D GGL RE+   + +  F+P    F+ +   ++      + IY+      + L ++ F+GR++ KA+Y+N L+E      F   +L K    V    ++S D   Y+ L +L  +   V DLG + T   E  E G T V +L  NG +I V+   + EY+ L+   K+   IR Q +AF +G  ++I    + +FN +EL+L++SG    +DV DL+ ++ Y   Y  +   +V FW  + S  +T K   ++FVT  S+ PL GF  L+       F +H       RLP+A TC N L LP +E    L+  LL AI E   GF  +
Sbjct:  656 IDVTIRRKFIYEDALEKLSPEAEPNMKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFRLTKDQRLYPNPSVHKIYE------DFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYDGCVQDLGLDFTIVNE--EVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKELD-----PPFCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAI-ESGSGFDLS 1016          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000008825 (supercontig:LSalAtl2s:LSalAtl2s54:414186:494517:-1 gene:EMLSAG00000008825 transcript:EMLSAT00000008825 description:"maker-LSalAtl2s54-augustus-gene-5.10")

HSP 1 Score: 159.458 bits (402), Expect = 2.033e-39
Identity = 116/376 (30.85%), Postives = 186/376 (49.47%), Query Frame = 0

Query: 1056 VHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEG-----EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQ--SQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKP----VKYTD-MESEDYSFYKGLEFL--IEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSG--LPNVDVDDLKANSEYH-KYQANSLQIVWFWRAL-RSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTD-------------------RLPSAHTCFNQLDLPAYETYDKLR 1394
            + V R  + ED +R L + S    K    + F       E G D  G+ +E+     + +F+P   LF ++  ++    +Y    S    N+L  F+FVG++I KA+Y+  ++E  F   F   IL+K       Y D + S D   YK L ++   +  V+DL  +LTFS +    G     +LI  GR++ V++  K+ Y+  V Q  + S I++Q++AF  GF  I+P   +++F+  E+  L+SG  LP +D+ DL+ ++ Y+  +  +   IVW W  L R FD  +++ F++FVT  SK PL GF  LE     +  ++  D+   D                   RLP+A TCFN L LP Y+    LR
Sbjct:  615 ITVHRSRIVEDGYRHLGKLSSRSLKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDPGLNLFCSTSDNR----LYPSPTSHLTENYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTDL--DLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLP-LDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNYQKKATLR 983          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000007808 (supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45")

HSP 1 Score: 127.872 bits (320), Expect = 3.217e-30
Identity = 113/428 (26.40%), Postives = 192/428 (44.86%), Query Frame = 0

Query:  995 FADTHRTV--LNQILRQSTTHLAD-------GPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQ-SQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKG-LEFLIEHTVSDLGYELTFSTEIR-EFG-VTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIV-WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGF 1408
            F   H T+  +  I +  T H          G F    +    + FD K   F +  E M E +      ++V R  +   S + L    Q  W     I F GE+G + GGL REWY ++ +E+             D   ++  ++ +  NP    Y  F                  F+ SF   +    +     + +   FY   +++++++ V DL  +L F+ EI  E G + E ++L P+G+ I V+   K EY+ L+ + +++  I+  L+ F  G +  +    ++ F+E E++LL SG   +D +DLK   ++H  Q  S +I+ WFW  L    +  K  F++F+TGSS++P  GF  L  +     F I R      +LPS+H C N L++  + +++   S  + AI E   GF
Sbjct:  291 FLLNHSTIFSITDIFKNKTLHFKLNERLFLLGSFVNAANKKCSMTFDEKLVEFFSNFEYMGELIFLMQSIINVDRHCLLYSSIKSL---EQCWWIGPLKISFSGEQGIEYGGLRREWYTLLWKEL------------SDTKIFITNERGTGANPMFRLYLPFS-----------------FSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVEDL--DLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLK---KHHNCQNVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDLCPV-----FTI-RQLNEEGKLPSSHVCANVLNIHPHSSFEAFESSFITAITEGYVGF 675          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000000248 (supercontig:LSalAtl2s:LSalAtl2s1036:68871:78712:-1 gene:EMLSAG00000000248 transcript:EMLSAT00000000248 description:"maker-LSalAtl2s1036-augustus-gene-0.34")

HSP 1 Score: 105.916 bits (263), Expect = 5.770e-23
Identity = 80/306 (26.14%), Postives = 138/306 (45.10%), Query Frame = 0

Query: 1121 SPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLI-----EHTVSDL--GYELTFSTEIR-EFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIV-----WFWRALRSFDQTDKAKFMQFVTGSSKVPL--QGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
             PG +  Y    Q +     L+ F+ VGR++   +  N+L   +  R   K+IL + +++ D+   D   Y+ L  L+     + T + L    +L FS ++  E G   V +LIPNGR +    +    YVR     +M     K LS    G Y+++P   +     ++  LLL+G+ +++V  L + + ++     S   +     W W+ +      +K   + F TGS  +P    GF  +  +        H        LPSA+TC ++L +P Y +   L+S LL AI+  ++ FGF 
Sbjct: 2706 CPGKRGFYSPV-QGKATQERLNAFRNVGRLMGLCLLQNELCPLFLNRHVIKYILGRTIRFHDLAFFDPVIYESLRQLVVDAENKETSAQLFSALDLNFSIDLSPEEGGGSV-ELIPNGREVEXXANNVYCYVRRYSYYRMIKCQEKALSNIKIGLYDVLPAGALDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGESQDRLVNFKRWLWQMVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSITIRPAGDSH--------LPSANTCISRLYVPLYSSKAILKSKLLLAIK--TKNFGFV 2999          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000002245 (supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12")

HSP 1 Score: 103.99 bits (258), Expect = 2.190e-22
Identity = 96/364 (26.37%), Postives = 154/364 (42.31%), Query Frame = 0

Query: 1061 ENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSP------GDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLI--------EHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYV------RLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQEC 1404
            E VF    +EL R     WK +      GE   DAGG+  +    + +E+   +  L   +P      G      + +  + +   + +F F+G +   A+   K +        +K IL     + D+E  D S+ + L ++         E ++    Y +  S   RE        L P    I +S + K EYV      R    M    A+RK LSA       + P  ++ +   Q +E L+ G  +  +  LK  + Y     ++  IVW W  L  F+   K  F+ FV+G S++P   F         Q+FQI R +R  + LP+A TCF QL LP Y + + +R+ L+ AI+ C
Sbjct: 2994 EQVFYIDPKEL-RLPYRSWKVKLV----GEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREV------PLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSA-------LAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDV------SQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHC 3333          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. L. salmonis genes
Match: EMLSAG00000009470 (supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20")

HSP 1 Score: 96.6709 bits (239), Expect = 3.776e-20
Identity = 100/398 (25.13%), Postives = 164/398 (41.21%), Query Frame = 0

Query: 1085 IVFEGEEGQDAGGLLREWYVIISREIFNPMYALFK-----------------TSPGDKVTYMIYDQSQTN--PNHL-------------DYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAK--PVKYTDMESEDYSFYKGLEFL---------------------IEHT---------VSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGL--PNVDVDDLKANSEY----HKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGP----QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGF 1408
            I +E E G   G  L E+Y ++S+E+      L+K                     DK + + Y  S     PN +             + F F+G+ IAKA+ DN++++  F++ FY+ +L +   +  +D+ + D +    +  L                     +E           + DLG + T        G   + DL   G++I V+ D   +YV LV    +   I  Q+ A  +GF ++ P   + +F  +ELE +  G    N +  D K   E     H + + S  + +    L S++  ++ +F+QFVTG  ++P+ GF AL   N P    +K   + D    D LPS  TC N L LP Y +   +R  L  A  E    F
Sbjct: 1354 IQYENEVGTGLGPTL-EFYTLVSKELQKADLGLWKGETVKISNEDVMDSNEDNERNDKSSCIEYIHSSVGLYPNPMARNVKSSQRSKIKNKFTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLP------GYPNI-DLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKAL---NPPLTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAMEGQYCF 1740          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|73915353|sp|Q7Z6Z7.3|HUWE1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=ARF-binding protein 1; Short=ARF-BP1; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Homologous to E6AP carboxyl terminus homologous protein 9; Short=HectH9; AltName: Full=Large structure of UREB1; Short=LASU1; AltName: Full=Mcl-1 ubiquitin ligase E3; Short=Mule; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1)

HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0
Identity = 483/802 (60.22%), Postives = 580/802 (72.32%), Query Frame = 0

Query:  656 LKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTP-GDNQLESGSAK-KGSFVDRF--TQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRR------------------AAKQSEKNAKKVVSQA-----------LKTEEPKEGEDSVKEDDEKMETDQDE--AEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNP-----------STSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            L+L+VEVLTS +CSEEGLED   +LL LS G   TRDT+LKLLL GA+ LG  + + +  L  ELRE   EQ +    E ++P G  + +  + K KG    RF   +  VI+ +  +  GG ELQLPSM+ LT+KTS+Q FFLRVL+VIIQLR+    + KK +                  A +Q E  A  ++                T E  + E SV+ ++  M+ DQ    A+       D   Q    + E        L  LSE+L L  LWD L  CLK+L ++ D HAVLVLQ  VEAFFLVHA     E + K   ++TR+ QLAHI+++  P        A PSSL+P           S+SL  DT KFL FA+THRTVLNQILRQSTTHLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE +R+ED+AVHVRR++VFEDS+RELHR+S EE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I   S  NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHIL K V+YTDMESEDY FY+GL +L+E+ VS LGY+LTFSTE++EFGV EVRDL PNG NI V+E+ K EYV LVCQM+MT AIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLP +D+DDLK+N+EYHKYQ+NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR  LL AIQECSEGFG A
Sbjct: 3581 LQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERP----PELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHAT----ERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA 4374          

HSP 2 Score: 355.91 bits (912), Expect = 4.960e-99
Identity = 230/522 (44.06%), Postives = 303/522 (58.05%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQ-------RRLQSMRQRAAAHVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQR-QAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRN-TFT-----GRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPP----AKIRKGATKTSGTLVETSTPTSVASS--SKPDRPTQWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSS 606
            LPEGVDPSFLAALP+D+R+EV+  Q       R   S    A A V   GV EV+PEFLAALPP IQEEVL QQR EQQR + A N + + P+DP  F+QTLP+ LR+SVL DME+S ++ +P D+AAEAQ LRRE E R R ++ ERLF H      SAL++ILR+  FT      RG QY        RG T    G +SH  P  S++          +      DHE LSCLL+LLF+++ K+NT+RLHR+LRNLCYH  TR WVI SLLSIL++S+E +            +  ETP      +  K ++K+ G+    + P  +     SK      WL++SMDAALG R N+FQ+QR GG+K +       STV IHP AA  V RH L+ LI LA+ FP HF   +        KET+  S +     G K+ +  S++ S +   T   D W+ L+KLD  + S+KGK+ V+S    +     E E+   +  +SP GQL++MLS  VIRRSS
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGH---SSTSALSAILRSPAFTSRLSGNRGVQYT--RLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLL-----DHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE------------LCIETPKLTTSEEKGKKSSKSCGSSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASGGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHFTQQRT-------KETNCESDR---ERGNKACSPCSSQSSSSGICT---DFWDLLVKLDNMNVSRKGKNSVKSVPVSAG---GEGETSPYSLEASPLGQLMNMLSHPVIRRSS 3447          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|341941147|sp|Q7TMY8.5|HUWE1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=E3Histone; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1)

HSP 1 Score: 949.888 bits (2454), Expect = 0.000e+0
Identity = 481/806 (59.68%), Postives = 584/806 (72.46%), Query Frame = 0

Query:  656 LKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTP-GDNQLESGSAK-KGSFVDRF--TQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRR------------------AAKQSEKNAKKVVSQA------------LKTEEPKEGEDSVKEDDEKMETDQ-----DEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNP-----------STSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            L+L+VEVLTS +CSEEGLED   +LL LS G   TRDT+LKLLL GA+ LG  + + +  L  ELRE   EQ +    E ++P G  + +  + K KG    RF   +  VI+ +  +  GG ELQLPSM+ LT+KTS+Q FFLRVL+VIIQLR+    + KK +                  A +Q E  A  ++                 +E   + E SV+ ++  M+ DQ      + + +  ++G  P+     E +        L  LSE+L L  LWD L  CLK+L ++ D HAVLVLQ  VEAFFLVHA     E + K   ++TR+ QLAHI+++  P        A PSSL+P           S+SL  DT KFL FA+THRTVLNQILRQSTTHLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE +R+ED+AVHVRR++VFEDS+RELHR+S EE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I   S  NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHIL K V+YTDMESEDY FY+GL +L+E+ VS LGY+LTFSTE++EFGV EVRDL PNG NI V+E+ K EYV LVCQM+MT AIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLP +D+DDLK+N+EYHKYQ+NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR  LL AIQECSEGFG A
Sbjct: 3582 LQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSNQSETSVRREESPMDVDQPSPSAQDTQSIVISDG-TPQGEKEKEEKPP-----ELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHAT----ERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA 4377          

HSP 2 Score: 352.058 bits (902), Expect = 8.420e-98
Identity = 227/524 (43.32%), Postives = 300/524 (57.25%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQ-------RRLQSMRQRAAAHVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQR-QAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRN-TFT-----GRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRK----GATKTSGTLVETSTPTSVASS--SKPDRPTQWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAG--SDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSS 606
            LPEGVDPSFLAALP+D+R+EV+  Q       R   S    A A V   GV EV+PEFLAALPP IQEEVL QQR EQQR + A N + + P+DP  F+QTLP+ LR+SVL DME+S ++ +P D+AAEAQ LRRE E R R ++ ERLF H      SAL++ILR+  FT      RG QY        RG T    G +SH  P  S++          +      DHE LSCLL+LLF+++ K+NT+RLHR+LRNLCYH  TR WVI SLLSIL++S+E +            +  ETP     +     ++K+  +    + P  +     SK      WL++SMDAALG R N+FQ+QR GG+K +       STV IHP AA  V RH L+ LI LA+ FP HF              T   +K+ N  S  +  +  +     + SS++G  +D W+ L+KLD  + S+KGK+ V+S    S     E E+   +  +SP GQL++MLS  VIRRSS
Sbjct: 2964 LPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGH---SSTSALSAILRSPAFTSRLSGNRGVQYT--RLAVQRGGTFQMGGSSSHNRPSGSNVDTLLRLRGRLLL-----DHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE------------LCIETPKLSTSEERGKKSSKSCASSSHENRPLDLLHKMESKSSNQLSWLSVSMDAALGCRTNIFQIQRSGGRKHTEKHASSGSTVHIHPQAAPVVCRHVLDTLIQLAKVFPSHF--------------TQQRTKETNCESDRERGSKQACSPCSSQSSSSGICTDFWDLLVKLDNMNVSRKGKNSVKSVPVSSG---GEGETSPHSLEASPLGQLMNMLSHPVIRRSS 3448          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|50403677|sp|P51593.2|HUWE1_RAT (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1)

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 256/322 (79.50%), Postives = 291/322 (90.37%), Query Frame = 0

Query: 1090 EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            EEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I   S  NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHIL K V+YTDMESEDY FY+GL +L+E+ VS LGY+LTFSTE++EFGV EVRDL PNG NI V+E+ K EYV LVCQM+MT AIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLP +D+DDLK+N+EYHKYQ+NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR  LL AIQECSEGFG A
Sbjct:    1 EEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGLA 322          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|597439636|sp|Q9P4Z1.4|TOM1_NEUCR (RecName: Full=E3 ubiquitin-protein ligase TOM1-like; AltName: Full=HECT-type E3 ubiquitin transferase TOM1-like)

HSP 1 Score: 473.781 bits (1218), Expect = 7.246e-138
Identity = 241/516 (46.71%), Postives = 325/516 (62.98%), Query Frame = 0

Query:  900 LWDALSGCLKDLADTPDH-HAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRRE--DLAVHVRRENVFEDSFRELHRRSQEEWK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            +WD LS CL  +    +  +   +L   +E+  +V    T   +        +++E L      L  P  ED  A                  F  F + HR +LN+++R +   L  G FS+LV + +VL+FD KR YF   +    +  R     L + VRRE+VF DSFR L+ +  +E K  +  I F+GEEG DAGG+ REW+ ++SR++F+P Y LF     D+ T+     S  N  HL +FKF+GR+I KA+Y+ +LLECYF+R+ YK IL KPV   DMES D  +YK L +++E+ ++D+  E TFS E   FG  +V DLI NGRNI V+E+ K EYVRL+ + K+ ++++ Q+ AFL GF+ IIP  LI+IFNEQELELL+SGLP++D+DD KAN+EYH Y A + QI WFWRA+RSFD+ + AK +QFVTG+SKVPL GF  LEGMNG  +F IHRD  S DRLPS+HTCFNQLDLP YE Y+ LRS LLKAI   S+ FGFA
Sbjct: 3586 MWDKLSACLSAIRQRDNMLNVATILLPLIESLMVV-CKNTTLSDASAVSNANSQKEML------LTSPPPEDRIAG----------------LFFTFTEEHRRILNELVRHNPK-LMSGTFSLLVKNPKVLEFDNKRNYFNRSVHSKYQQTRHSFPPLQLQVRREHVFHDSFRSLYYKKADELKFGKLNIRFQGEEGVDAGGVTREWFQVLSRQMFDPNYVLFVPVSSDRTTFHPNKLSPINDEHLPFFKFIGRIIGKALYEGRLLECYFSRAVYKRILGKPVSVKDMESFDPDYYKSLVWMLENDITDIITE-TFSVEDDVFGEVKVVDLIENGRNIPVTEENKHEYVRLIVEHKLITSVKDQMKAFLTGFHEIIPEELIAIFNEQELELLISGLPDIDIDDWKANTEYHNYSAGAPQIQWFWRAVRSFDKEELAKLLQFVTGTSKVPLNGFKELEGMNGVSRFNIHRDYGSKDRLPSSHTCFNQLDLPEYENYETLRSQLLKAITAGSDYFGFA 4076          

HSP 2 Score: 98.9821 bits (245), Expect = 2.629e-19
Identity = 96/280 (34.29%), Postives = 134/280 (47.86%), Query Frame = 0

Query:  100 MDISELPEGVDPSFLAALPEDMRQEVIDE---QRRLQSMRQRAAAHVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQ----AAANQN-PEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRP---LLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILE 368
            +DI+EL  G+D  +L ALPE+ R EVI +    RR Q+ R++ +   E T   EV  EFL ALP  ++ E+LHQ++ EQ+R+    AA  Q+   A +DP   L T P  LRQ VL D  E  M  L  +LAAEA+TL      R+R +       H  QGG +A                          R+RD    T   + G  ++         KI+ R    +LD +G++ LL L+F+       + L  I  NLC +   R  VI SLL IL+
Sbjct: 2969 IDITEL--GIDAEYLEALPEEFRDEVIAQAISTRRSQA-REQVSQEGENT---EVFQEFLEALPEELRNEILHQEQHEQRRRERQNAAGGQDLGPADMDPASILLTFPPGLRQQVLLDQGEDIMEHLGPELAAEARTLV----ARHRQL-------HAQQGGQAA-------------------------SRSRDAQRPT---EAGAGTVQ--------KIQKRTVVQMLDKQGIATLLRLMFVSQQGSIRSSLFSIFANLCENRQNRLDVISSLLQILQ 3195          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|442570058|sp|Q756G2.2|TOM1_ASHGO (RecName: Full=Probable E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1)

HSP 1 Score: 452.981 bits (1164), Expect = 6.172e-131
Identity = 236/530 (44.53%), Postives = 331/530 (62.45%), Query Frame = 0

Query:  884 ILESLESLSEELELQHLWDALSGCLKDLADTPDHH-AVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            +++ L  L  ++EL H+W +LS CL    + P    +  +L   +E+  +V       E K   L+ E ++   A                       P  +L      F  F D H+ +LN+++R S   L  GPFS+LV + ++LDFD KR YF  +L  +  +  R  L++ VRRE+VF DS+R L  +S E+ K ++  I F+GE G DAGG+ REWY ++SR++FNP YALF     DK T+     S  NP HL +FKF+G +I KAI D   L+C+F+R  YK+IL KPV   DMES D  +YK L +++E+ ++D+  E TFS E  ++G  +V +LI NG ++AV+E  K +YV+ + + K+ ++++ Q+  FL+GFY IIP+ LISIF+EQELELL+SGLP++DVDD K N+ Y  Y     QI +FWRA+RSFD+ ++AK +QFVTG+SKVPL GF  L G+NG  KF IHRD  S DRLPS+HTCFNQLDLPAY++Y+ LR  LL AI E  EGFG A
Sbjct: 2760 VVDQLLPLYNKMELGHIWVSLSNCLTRFEEKPRMSTSATILLPLIESLMVVCKHSKVRETKDALLKYEAKKCDFAR---------------------TPVENL------FFAFTDLHKKLLNEMIR-SNPKLMSGPFSLLVKNPKILDFDNKRYYFTAQLRAITHD--RPKLSISVRREHVFLDSYRSLFFKSNEDIKISKLEISFKGEAGVDAGGITREWYQVLSRQMFNPDYALFIPVASDKTTFRPNRTSGINPEHLSFFKFIGMIIGKAISDQCFLDCHFSREVYKNILGKPVALKDMESLDLDYYKSLIWILENDITDI-IEETFSVETDDYGEHKVIELIENGAHVAVTEQNKHDYVKKIVEYKLQTSVKDQMENFLQGFYAIIPKDLISIFDEQELELLVSGLPDIDVDDWKNNTIYVNYTPTCKQINYFWRAVRSFDKEERAKLLQFVTGTSKVPLNGFKELSGVNGISKFSIHRDYGSIDRLPSSHTCFNQLDLPAYDSYETLRGSLLLAINEGHEGFGIA 3258          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|172045749|sp|Q8GY23.3|UPL1_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL1; Short=Ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase UPL1)

HSP 1 Score: 437.958 bits (1125), Expect = 6.466e-126
Identity = 245/551 (44.46%), Postives = 340/551 (61.71%), Query Frame = 0

Query:  897 LQHLWDALSGCLKDLADTPDHHA----------VLVLQAT-------------VEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPN------HLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIRE-----FGVTEVRD--LIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            L+ LW  LS C+       DH A          VL +  T             +EAFF++      P      L+++T       ++E      S  H ++  +S++ S   T  +  F  FA+ HR +LN  +RQ+ + L +   S+++   R++DFD K+ YFR+ +    +      L + VRR  V EDS+ +L  RS ++ K R  + F+GEEG DAGGL REWY ++SR IF+    LF T+ G+  T+      Q NPN      HL YFKFVGR++AKA++D +LL+ YFTRSFYKHIL   V Y D+E+ D  +YK L++L+E+ VSD+  +LTFS +  E     +  TEV D  L P GRNI V+E+TK EYV LV    +T+AIR Q++AFLEGF  +IPR L+SIFN++ELELL+SGLP +D DDLKAN+EY  Y A S  I WFW  +++F + D A+F+QFVTG+SKVPL+GF AL+G++GPQ+ QIH+   + +RLPSAHTCFNQLDLP Y++ ++L+  LL AI E SEGFGFA
Sbjct: 3152 LEPLWHELSQCISMTELQLDHTAAASNINPGDHVLGISPTSSLSPGTQRLLPLIEAFFVLCEKIQTP----SMLQQDTNVTA-GEVKE------SSAHGSSSKTSVD-SQKKTDGSVTFSKFAEKHRRLLNSFIRQNPS-LLEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLF-TTVGNDATF------QPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGFGFA 3681          

HSP 2 Score: 98.5969 bits (244), Expect = 3.515e-19
Identity = 92/270 (34.07%), Postives = 131/270 (48.52%), Query Frame = 0

Query:  108 GVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHV---NQGGNSALNSILRNTFTG--RGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNE 372
             +DP+FL ALPED+R EV+  Q+  QS++       E   V +++PEFLAALPP+IQ EVL QQR   QR    +Q     +D    + TLP  LR+ VL    E+ ++ALP+ L AEAQ L      R+RAM   +  + V   +   N+  N +  N  TG  RG    IG     R  +   DG                     +I+G PL++ + L  L+ LL +    +    L R+L NLC H  TR  ++  LL ++    E
Sbjct: 2608 AIDPTFLEALPEDLRAEVLASQQA-QSVQ---PPTYEPPPVDDIDPEFLAALPPDIQTEVLAQQR--AQRMVQQSQGQAVDMDNASIIATLPADLREEVLLTSSEAVLAALPSPLLAEAQML------RDRAMSHYQARSSVFGSSHRLNNRRNGLGYNRLTGMDRGVGVTIG----QRAVSSSADGLKVK-----------------EIEGDPLVNADALKSLIRLLRLAQ-PLGKGLLQRLLLNLCAHSFTRANLVQLLLDMIRPEME 2843          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|50400805|sp|O13834.1|PTR1_SCHPO (RecName: Full=E3 ubiquitin-protein ligase ptr1; AltName: Full=HECT-type E3 ubiquitin transferase ptr1; AltName: Full=Poly(A)+ RNA transport protein 1)

HSP 1 Score: 437.573 bits (1124), Expect = 6.864e-126
Identity = 231/531 (43.50%), Postives = 325/531 (61.21%), Query Frame = 0

Query:  883 DILESLESLSEELELQHLWDALSGCLKDLAD-TPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            +I++ LE   + L+   LW+ LS CL  L D T   H   VL   +E+  ++           + +  E  ++   HI   LE  K+                       F+ F + HR ++N ++  +T  L  G FS+LV + +VL+F+ KR YF  +L       +   L + VRR++VF DS+R LH +  +E K ++  I F  EEG DAGG+ REW  +++R++FNP YALF    GD  T+     S  NP+HL +FKF GR+I KA+YD +LL+C+F+R+ YKH+L + V   D+ES D  +YK L +++ + ++D+  E  F+ E   FG   V DLIPNGRNI V+E  K  YV  +   K+  +++ QL + L+GF +IIP  LI IFNEQELELL+SGLP +D+DD K N+EYH Y  +S Q+ WFWRA+RSFD+ ++AK +QF TG+SKVPL GF  LEGM+G Q+F IH+   S +RLP +HTCFNQLDLP Y+TY++LRS LL AI E SEGFGFA
Sbjct: 2732 NIIQLLE-FYDNLKFSSLWEVLSECLSALRDHTSITHVSTVLLPLIESLMVI----------CRLVFIELPEDVGKHISPILERFKT----------------------LFISFTEEHRKIINMMVF-TTPSLMSGSFSLLVKNPKVLEFENKRNYFNRQLHEEAAKEQYPPLNITVRRDHVFLDSYRALHFKDADEVKFSKLNIHFRDEEGVDAGGVTREWLQVLARQMFNPDYALFLPVTGDATTFHPNRDSSVNPDHLSFFKFTGRIIGKALYDGRLLDCHFSRAVYKHMLHRSVSVKDIESLDPDYYKSLVWMLNNDITDIITE-EFAVEKDVFGEKTVVDLIPNGRNIPVTELNKQNYVNRMVDYKLRESVKDQLKSLLDGFSDIIPSHLIQIFNEQELELLISGLPEIDIDDWKNNTEYHGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKELEGMSGFQRFNIHKSYGSLNRLPQSHTCFNQLDLPEYDTYEQLRSMLLTAINEGSEGFGFA 3227          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|332278235|sp|Q8H0T4.3|UPL2_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL2; Short=Ubiquitin-protein ligase 2; AltName: Full=HECT-type E3 ubiquitin transferase UPL2)

HSP 1 Score: 432.18 bits (1110), Expect = 4.856e-124
Identity = 218/433 (50.35%), Postives = 296/433 (68.36%), Query Frame = 0

Query:  992 FLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPN------HLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIRE-----FGVTEVRD--LIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            F  F + HR +LN  +RQ+ + L +  FS+++   R++DFD K+ YFR+ +    +      L + VRR  V EDS+ +L  RS ++ K R  + F+GEEG DAGGL REWY ++SR IF+    LF T+ G+  T+      Q NPN      HL YFKFVGR++AKA++D +LL+ YFTRSFYKHIL   V Y D+E+ D  +YK L++L+E+ VSD+  +LTFS +  E     +  TEV D  L P GRNI V+E+TK EYV LV    +TSAIR Q++AFLEG   +IPR L+SIFN++ELELL+SGLP +D DDLKAN+EY  Y   S  I WFW  +++F + D A+F+QFVTG+SKVPL+GF AL+G++GPQ+ QIH+   S +RLPSAHTCFNQLDLP Y++ ++++  LL AI E +EGFGFA
Sbjct: 3235 FSKFVEKHRRLLNSFVRQNPS-LLEKSFSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLF-TTVGNDATF------QPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGFGFA 3658          

HSP 2 Score: 85.8853 bits (211), Expect = 2.388e-15
Identity = 93/270 (34.44%), Postives = 127/270 (47.04%), Query Frame = 0

Query:  108 GVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLR----REFEIRNRAM-IQERLFNHVNQGGNSALNSILRNTFTGRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNE 372
             +DP+FL ALPED+R EV+  Q+  QS++       E   V +++PEFLAALPP IQ EVL QQR   QR    +Q     +D    + TLP  LR+ VL    E+ ++ALP  L AEAQ LR    R ++ R+R      RL N  N  G        R T   RG    IG     R  +   DG                     +I+G PL++ + L  L+ LL +    +    L R+L NLC H  TR  ++  LL ++    E
Sbjct: 2592 AIDPTFLEALPEDLRAEVLASQQA-QSVQ---PPTYEPPSVDDIDPEFLAALPPEIQREVLAQQR--AQRMLQQSQGQPVDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLAEAQMLRDRAMRHYQARSRVFGSSHRLNNRRNGLG-------YRLTGMERGVGVTIG----QRDVSSSADGLKVK-----------------EIEGDPLVNADALKSLIRLLRLAQ-PLGKGLLQRLLLNLCAHSFTRANLVQLLLDMIRPEME 2826          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|50401412|sp|Q03280.1|TOM1_YEAST (RecName: Full=E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1; AltName: Full=Suppressor of snRNA protein 2; AltName: Full=Temperature-dependent organization in mitotic nucleus protein 1)

HSP 1 Score: 431.409 bits (1108), Expect = 9.038e-124
Identity = 212/429 (49.42%), Postives = 295/429 (68.76%), Query Frame = 0

Query:  984 SLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKN-RFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            S T+  + F  F D H+ +LNQ++R S   L  GPF++LV + +VLDFD KR +F  +L+   +N  R  L + VRRE VF DS+R L  ++ +E KN +  I F+GE G DAGG+ REWY ++SR++FNP YALF   P DK T+     S  NP HL +FKF+G +I KAI D   L+C+F+R  YK+IL +PV   DMES D  +YK L +++E+ ++D+  E TFS E  ++G  +V +LI  G++I V+E  K +YV+ V + K+ +++++Q+  FL GFY +I + LI+IF+EQELELL+SGLP++DVDD K N+ Y  Y A   ++ +FWRA+RSFD  ++AK +QFVTG+SKVPL GF  L G+NG  KF IHRD  S++RLPS+HTCFNQL+LP YE+Y+ LR  LL AI E  EGFG A
Sbjct: 2844 SKTRVENLFFPFTDAHKKLLNQMIR-SNPKLMSGPFALLVKNPKVLDFDNKRYFFNAKLKS--DNQERPKLPITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDI-IEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFGLA 3268          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. SwissProt
Match: gi|1008808725|sp|G0S9J5.2|RSP5_CHATD (RecName: Full=E3 ubiquitin-protein ligase RSP5; AltName: Full=HECT-type E3 ubiquitin transferase RSP5)

HSP 1 Score: 340.117 bits (871), Expect = 4.822e-99
Identity = 167/382 (43.72%), Postives = 245/382 (64.14%), Query Frame = 0

Query: 1030 DFDIKRRYFRTELERMDENMRREDLAVH--VRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFG 1409
            DF  K  YFR++       MR      H  VRR ++FED+F E+ R+S  + K R  I F+GE+G D GGL RE++ ++S E+FNP Y LF+ S  D  T  I   S  NP HL+YFKF+GRV+  AI+  + L+ +F  +FYK IL KPV   DME  D  F++ L++++++ +S    E TFSTE   FGV  V DL PNGRNI V+ + K EYV L+ + ++   + +Q  AF EGF  +IP+ LI++F+E+ELELL+ G+  +DVDD K +++Y  Y  +   I +FW+ +RS+D   K++ +QF TG+S++P+ GF  L+G +GP++F I R     + LP AHTCFN+LDLP Y+T ++L+  L  A++E + GFG
Sbjct:  444 DFRRKLIYFRSQ-----PAMRILSGQCHIKVRRSHIFEDAFAEISRQSATDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGINPEHLNYFKFIGRVVGLAIFHRRFLDAFFITAFYKMILGKPVTLADMEGVDADFHRSLQWMLDNDISGGIIEATFSTEDERFGVITVEDLKPNGRNIEVTNENKREYVELMVKWRIQKRVEEQFKAFKEGFNELIPQDLINVFDERELELLIGGIAEIDVDDWKKHTDYRGYTESDEVIQFFWQTVRSWDSEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIERAG-DINNLPKAHTCFNRLDLPPYKTLEQLQQKLTMAVEE-TMGFG 818          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1325322365|ref|XP_023343002.1| (E3 ubiquitin-protein ligase HUWE1-like [Eurytemora affinis])

HSP 1 Score: 1535.39 bits (3974), Expect = 0.000e+0
Identity = 872/1396 (62.46%), Postives = 1002/1396 (71.78%), Query Frame = 0

Query:   68 QAGPSGLAGAIGSSASETGAPSTEQDYATILGMDISELPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAH--VETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRGSQYV---IGGFGNSRGRTRDRDGWTSHIDPGVSSLHQ------AYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPTQWLNISMDAALGFRANVFQVQRL----GGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQS-TSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQ---EAIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKK----------RRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVA----------------------KTEGDQPEQAASSESEESLDILES-LESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            +AGPSG +    + A  +G      D++ ILG DI E+PEGVDPSFLAALPEDMRQEV++EQRRL   RQ       ++T GV EVN EFLAALPPNIQEEVL QQR+EQQRQ+AA  +P APVDPGEFLQTLP SLRQS+LA+MEESQ+SALPA++AAEAQTLRR+FE RNRAM+ ER F+HVN    + L+SILRNT    G   V    GG G        R  W+  I  G  S HQ      A  T + K KGR LLDHE LSC+LILLFI+DAK+NT RLHRILRNLCYH PTRDWVI  LLSILEK+N       +             PAK++K  +K   T  E      V  SS+    T WLNISMDAALGFRANVFQVQR     GGK+SS++     + + IHP AA  V ++TLEVLISLA+SF  HFLP                          +S   D+ + +     T   + WETLLKLD +  TS+KGKSVVRSHS+   +K E+D+S S + + S FGQLL ML+  VI+RSS+LTDKLLRLLSLIS+GQP++ K  ++         SS  Q Q     +  D +KLAVEVLTSKACSEEGLEDV ALLLNLSY    TR++IL LLL GA+ELGNVVR +V  L  EL +LK+       S  +  G  + E  S  KG   DRFT+++++LTAPTK K G+ELQL SM++LT KTSSQ+FFLRVLKVIIQLREAAL ++KK            AAK++   A  +    L  E P+   DS     E  ET  D AE  A                      K E +  E   S E  +  D  E  LESLS++L L  LW+ LS CL++LADTPDHHAVLVLQ TVEAFFLVHAA T  +EKKKT +KETRQEQLAHIQE+    K  D +    + + P   L+ DT KFL FA+THRTVLNQILRQSTTHLADGPF+VLVDHTRVLDFDIKRRYFRTELER+DE MRREDLAVHVRRE VFE+SFRELHRRS EEWKNRFYIVFEGEEGQDAGGLLREWY+IISREIFNP YALFK+SPGD+VTY I D S  N NHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPV + DMESEDY FYKGL+FL+E+ VSDLGY+LTF+TEI+EFGVTEVRDLIPNGRNI V+E+ K +YVRLVCQMKMT AIRKQL AFLEGFY+IIPRRLISIFNEQELELLLSGLP++++DDLK N+EYHKY A SLQIVWFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF ALEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLR+YLLKAIQECSEGFGFA
Sbjct: 2782 EAGPSGAS----APAERSGI-----DFSEILG-DI-EIPEGVDPSFLAALPEDMRQEVLEEQRRLLRARQAPPPPPTLQTQGVAEVNAEFLAALPPNIQEEVLAQQRLEQQRQSAAQVDPTAPVDPGEFLQTLPPSLRQSLLAEMEESQISALPAEIAAEAQTLRRDFEQRNRAMMHERFFSHVNHPA-ATLSSILRNTVNRMGGYTVHSSTGGPG--------RSVWSRSIGRGGPS-HQHSAALIAAQT-SAKFKGRQLLDHEALSCILILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVIKCLLSILEKANCCTDSSSALTADPLQPGASPTPAKLKKSTSKGGSTPGE-----HVRGSSQ----TSWLNISMDAALGFRANVFQVQRPSSTGGGKRSSNVI--NIANINIHPQAAQVVCKNTLEVLISLAKSFSIHFLP-------------------------SQSKEKDAGEGTSETKKTKLPEFWETLLKLDKECWTSRKGKSVVRSHSS-VGIKSEDDDSFSASSSVSAFGQLLGMLASPVIKRSSLLTDKLLRLLSLISLGQPEVVKRHEEPDTL-----SSLEQVQGFDRTVREDQIKLAVEVLTSKACSEEGLEDVTALLLNLSYVGTQTRESILHLLLAGARELGNVVRDHVSVLLNELADLKS-------SGNLVVGIKEEEDESKHKGVLADRFTREAIVLTAPTKPKAGAELQLSSMSALTNKTSSQSFFLRVLKVIIQLREAALHAMKKSLKAKKDAEAFAAAKKAAAAAFALTGPQLPAENPEATPDSGNIPSESTETPIDSAEVPADKADVPTVTTDTPTEVGEEEDTKMEVEPSEPTQSEEQTKPKDDDEGHLESLSDQLALSMLWETLSACLRELADTPDHHAVLVLQPTVEAFFLVHAAATTNQEKKKTNQKETRQEQLAHIQEE----KLTDDSVGSTTVVAP---LSPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFAVLVDHTRVLDFDIKRRYFRTELERLDEGMRREDLAVHVRREAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYMIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVSHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFATEIQEFGVTEVRDLIPNGRNITVTEENKNDYVRLVCQMKMTGAIRKQLGAFLEGFYDIIPRRLISIFNEQELELLLSGLPDINIDDLKGNTEYHKYTATSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGSQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRNYLLKAIQECSEGFGFA 4099          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|815816232|ref|XP_012229199.1| (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1 [Linepithema humile])

HSP 1 Score: 1459.89 bits (3778), Expect = 0.000e+0
Identity = 775/1319 (58.76%), Postives = 937/1319 (71.04%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSN-EGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPTQ---WLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETS-TSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHST---GSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQEAIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ MRQRA A V  E +G  EVNPEFLAALPP IQEEVL QQRMEQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR M+QER  NHV+Q  N+AL+SILRN+   RG     GG   +      R  W +    G +++  A+ +  ++++GR LLDHE ++CLL+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN E K +       +A             G  K  G L   + P  + S    D+      WLNIS+DAALG RANVF + R  GKKS      + + + IHP AA  V RHTLE+LISLA+SFPG+F+P K        KE+    S K    SG K   G  NK +  +  +   D W+ LLKLD    SKKGKSV R+HS    GS     E E  +  F +S FGQL+SML++SVI+RSS LTDKLLRLLSLIS+G  +M    + + +    +   +     A P   LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL N+V +++  L  EL+ L  E  +  + E     D    SG   +G  +DRFT ++V++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+   ++A  Q+ + A    + +  +E   E  D+    D  M+T Q     +     D  +  ++   EE      +L  LSE L L +LW+ LS CL +L  TPDHHAVLVLQ  VEAFFLVH++ +   +++  L   T   ++  +   + P  P ++  +     +  P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR +VFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH +SDLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E++K++Y+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DL+AN+EYHKY A SLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2968 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRMRQRAQASVVEELSGPVEVNPEFLAALPPAIQEEVLAQQRMEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHMMQERFLNHVSQS-NTALSSILRNSGRARG-----GGTRYAIHAVPQRSQWNAWNSRGDTNM--AHQSAGLRLRGRQLLDHESMACLLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVKALLSIMERSNDENKDI-------MADFG----------GKFKRKGLL---NPPMDLCSPKIFDQRNNIQSWLNISIDAALGCRANVFHIIRHAGKKSER----QGNIIAIHPQAAPTVCRHTLELLISLAKSFPGYFVPIKS-------KESDDKDSNKEKDVSGSKEEGGAKNKSASKVGRSDLPDFWDLLLKLD-SCASKKGKSVARTHSNTNLGS-----EPEHGTTTFEASAFGQLISMLNWSVIKRSSQLTDKLLRLLSLISIGLTEMNPYRRQESSVKNKKLELNKDQSVAAPEGHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANMVCEHINDLMMELKVLNRELRRRNSLE-----DQPSTSGGNGRGILLDRFTNENVVVTAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLA-NTKKAVNQTSETAMDTGTTSNASETATENADA----DVPMDTTQANTSNL-----DNSKTTSTRNDEEK----TTLPLLSESLVLDNLWETLSACLLELEYTPDHHAVLVLQPAVEAFFLVHSSSSTSRDRESRLNTNTENREVPDLAP-VSPIYPDNDGQSEVAVGTWEPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRSHVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNISDLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEESKLDYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLRANTEYHKYTATSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGVQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4221          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1366088310|ref|XP_024222245.1| (E3 ubiquitin-protein ligase HUWE1 isoform X2 [Bombus impatiens])

HSP 1 Score: 1459.51 bits (3777), Expect = 0.000e+0
Identity = 774/1328 (58.28%), Postives = 927/1328 (69.80%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   SH DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+                 E     +  G      +LV        A      R +   WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K  TG   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT ++V++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE +                                           D      +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   ETR+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2925 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSHGDPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-----------------ENKDIAMDFGGKFRRKSLVPMMHHDYCAPKIFDQRSSAQSWLNISIDAALGCRANVFHIVRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEETGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDSEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSVAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKILNRELRRNSIEEQPS------TSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSNQSSESSV------------------------------------------DTGNTNQASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSISRDRHLSTNTETRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPPPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4147          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|815906178|ref|XP_012238909.1| (E3 ubiquitin-protein ligase HUWE1 isoform X1 [Bombus impatiens])

HSP 1 Score: 1458.74 bits (3775), Expect = 0.000e+0
Identity = 774/1328 (58.28%), Postives = 927/1328 (69.80%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   SH DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+                 E     +  G      +LV        A      R +   WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K  TG   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT ++V++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE +                                           D      +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   ETR+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2930 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSHGDPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-----------------ENKDIAMDFGGKFRRKSLVPMMHHDYCAPKIFDQRSSAQSWLNISIDAALGCRANVFHIVRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEETGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDSEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSVAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKILNRELRRNSIEEQPS------TSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSNQSSESSV------------------------------------------DTGNTNQASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSISRDRHLSTNTETRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPPPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4152          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|808127135|ref|XP_012166531.1| (E3 ubiquitin-protein ligase HUWE1 isoform X1 [Bombus terrestris])

HSP 1 Score: 1457.58 bits (3772), Expect = 0.000e+0
Identity = 774/1328 (58.28%), Postives = 924/1328 (69.58%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPSSLNPSTS----------LTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   SH DP        +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+                 E     +  G      +LV        A      R +   WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K  TG  +K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT ++V++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE +                                           D      +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   ETR+     +   L P  P   D+     S    +TS          L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2931 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSHGDP------ITHHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-----------------ENKDIAMDFGGKFRRKSLVPMMHHDYCAPKIFDQRSSAQSWLNISIDAALGCRANVFHIVRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEETGSKSKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDSEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSVAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSNQSSESSV------------------------------------------DTGNTNQASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTNTETRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTSQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4152          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1185577234|ref|XP_020719766.1| (E3 ubiquitin-protein ligase HUWE1 isoform X2 [Bombus terrestris])

HSP 1 Score: 1456.81 bits (3770), Expect = 0.000e+0
Identity = 774/1328 (58.28%), Postives = 924/1328 (69.58%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPSSLNPSTS----------LTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   SH DP        +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+                 E     +  G      +LV        A      R +   WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K  TG  +K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT ++V++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE +                                           D      +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   ETR+     +   L P  P   D+     S    +TS          L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2926 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSHGDP------ITHHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-----------------ENKDIAMDFGGKFRRKSLVPMMHHDYCAPKIFDQRSSAQSWLNISIDAALGCRANVFHIVRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEETGSKSKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDSEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSVAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSNQSSESSV------------------------------------------DTGNTNQASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTNTETRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTSQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4147          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|820863765|ref|XP_012348525.1| (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1 [Apis florea])

HSP 1 Score: 1456.81 bits (3770), Expect = 0.000e+0
Identity = 778/1326 (58.67%), Postives = 933/1326 (70.36%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I          R    WT+  DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+        N  +A + F     KIR+       +LV         S     R     WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K   G   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT +SV++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE         ++ TE                 T+Q                  +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   E R+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2927 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAIHTVAQ-----RGWQPWTNG-DPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-------ENXDIA-MDFG---GKIRRK------SLVPMMHHDYCCSKIFDQRSNAQSWLNISIDAALGCRANVFHIVRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKENNKXKDSSNKEEAGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDTEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDMNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSES--------SVDTE----------------NTNQ------------------ASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4149          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1241829656|gb|PBC26112.1| (E3 ubiquitin-protein ligase [Apis cerana cerana])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.000e+0
Identity = 779/1328 (58.66%), Postives = 935/1328 (70.41%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   S+ DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+        N  +A + F     KIR+ +      LV        A      R     WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K   G   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT +SV++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE         ++ TE                 T+Q                  +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   E R+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2926 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSNGDPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-------ENKDIA-MDFG---GKIRRKS------LVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEEIGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDTEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSES--------SVDTE----------------NTNQ------------------ASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4148          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1035587454|ref|XP_016910514.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X2 [Apis cerana])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.000e+0
Identity = 779/1328 (58.66%), Postives = 935/1328 (70.41%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   S+ DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+        N  +A + F     KIR+       +LV        A      R     WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K   G   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT +SV++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE         ++ TE                 T+Q                  +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   E R+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2926 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSNGDPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-------ENKDIA-MDFG---GKIRRK------SLVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEEIGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDTEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSES--------SVDTE----------------NTNQ------------------ASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4148          

BLAST of e3 ubiquitin-protein ligase huwe1-like isoform x5 vs. nr
Match: gi|1035587452|ref|XP_016910505.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X1 [Apis cerana])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.000e+0
Identity = 779/1328 (58.66%), Postives = 935/1328 (70.41%), Query Frame = 0

Query:  105 LPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAAHV--ETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRG--SQYVIGGFGNSRGRTRDRDGWT--SHIDPGVSSLHQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASVSFETPPAKIRKGATKTSGTLVETSTPTSVASSSKPDRPT--QWLNISMDAALGFRANVFQVQRLGGKKSSSLSGDKTSTVGIHPGAATFVSRHTLEVLISLARSFPGHFLPWKDAADLFLLKETSTSSKKFNPTSGGKSSTGDSNKKSENLSSTAGSDLWETLLKLDLQSTSKKGKSVVRSHSTGSSLKLEEDESQSVNFNSSPFGQLLSMLSFSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSSNQAQE-AIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKVVSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEP--PKSEDHAAAGPS--SLN--------PSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411
            LP+GVDPSFLAALPEDMR EVI EQ RLQ +RQRA A V  E TG  EVNPEFLAALPP IQEEVL QQR+EQQR AAA+ NPE PVD   F Q L  SLRQ++L DMEESQ+S LP DLA EAQ LRRE+E RNR ++Q+R  NHV+QG N+AL+SILRN+  GRG  ++Y I         T  + GW   S+ DP ++     +H   ++++GR LLDHE ++C+L+LLF+++ KINT RLHR++RNLCYH  TR+WV+ +LLSI+E+SN+        N  +A + F     KIR+       +LV        A      R     WLNIS+DAALG RANVF + R GGKKS      + S++ IHP AA  V RHTLE+LISLA+ FPG+F+P K        KE+          S  K   G   K +     +   D W+ LLKLD    SKKGKSV RSHS  S+L   + E  +  F SS FGQL+SML++SVI+RSS LTDK+L+LLSLIS+G  ++      ++  N+ + +  N+ Q  A P + LKLAV+VLTSK+CSEEGLED  ALLLNLS+ P+PTR  ILKLLL+GA EL NVV +++  L  EL+ L  E  ++   E  +       S    +   +DRFT +SV++TAP+KVK GS+LQLPSM +L +KTSSQAFFLR+LKVI+Q+REA  L+  K+ + + SE         ++ TE                 T+Q                  +SES E     + L  LSE L L HLW+ LS CL +L  TPD HAVLVLQ  VEAFFLVH++ +   ++  +   E R+     +   L P  P   D+     S  ++N        P  +L  D  KFL FA+THRTVLNQILRQ+TTHLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFEDSFRELHRR+ +EWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF  SPGD+VTYMI   S  NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHIL   VK+TDMESEDYSFYKGL +L EH ++DLGYELTFSTE+ EFGV +VRDLIPNGRNI V+E+TK+EY+RLVCQMKMT AIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLPNVD++DLKAN+EYHKY ANSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGF+ALEGMNG QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLR+ LLKAI ECSEGFGFA
Sbjct: 2931 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQG-NAALSSILRNSGRGRGGGTRYAI--------HTVAQRGWQPWSNGDPSIT-----HHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSND-------ENKDIA-MDFG---GKIRRK------SLVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSER----QGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKS-------KESDEKESNKEKDSSNKEEIGSKGKSTSKPGKSDQPDFWDMLLKLD-SCASKKGKSVARSHS-NSNLG-NDTEHNTTTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYR--RQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPS------TSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSES--------SVDTE----------------NTNQ------------------ASESGE-----DKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENRE-----VVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA 4153