P-glycoprotein, snap_masked-scaffold344_size201325-processed-gene-0.9 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000010048 (supercontig:LSalAtl2s:LSalAtl2s662:116216:125875:1 gene:EMLSAG00000010048 transcript:EMLSAT00000010048 description:"snap_masked-LSalAtl2s662-processed-gene-1.13") HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0 Identity = 319/593 (53.79%), Postives = 407/593 (68.63%), Query Frame = 0
Query: 231 MTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAI----DSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG-ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSLVMAQTKKEETRDG---------EDEIDEIQDLETVDQEISEM-------GTAGPRN----RSQSERRPSVASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDR 785
++ DLNK+QEG+GEKIG+FIFF TIF++SLI AF+HGWELTLVI S MP+L+I+ IIA SQT LT +E AY KAGSVAEE SS++TV++F GQ+ E+ R++ NL A+K GI+RG+LTG+GGG MW +IY SYA+AFWYGVKLI+DD E C + +C RY S+L++VFFSVLMGAMN+GQA+PY+EAF++ARGAA+ ++ +I TPAI D D P G +TFK+ VL GL+ + G+T+ALVGPSGCGKSTVIQL+QRFYDP G V ++G DI+ L LR IGIVGQEPVLF +I +NI G +G I++K I+ CK ANAY FIQ LPKKY+T+VGE+GA +SGGQKQRIAIARAL+RNP ILLLDEA+SALDSQSE +VQSALDKAR GRTTI+VAHR LSTIR+AD I+ +KDG + GTH L N+ G+Y SLV AQ + + +G +E DE+ DLE V+ E++ M G++ R R ERR S S +S+DS +ED GSA+G++RV S +
Sbjct: 11 LSMDLNKLQEGIGEKIGLFIFFITIFISSLITAFIHGWELTLVILSAMPILMIAVGIIAKSQTALTVKESNAYSKAGSVAEEAFSSIKTVMSFQGQNTEIQRYKENLSEAQKTGILRGLLTGIGGGLMWFIIYSSYAIAFWYGVKLILDDRESCIASPTDCQIRYGPSNLLIVFFSVLMGAMNIGQASPYVEAFAIARGAASSVFQIIQSTPAIKSDYDHLQRQQDKAPPFTGRITFKNVHFEYPSRPTVKVLSGLSFEASPGKTLALVGPSGCGKSTVIQLIQRFYDPSFGVVSIDGEDITTLDPHWLRSHIGIVGQEPVLFEYSIKENITMGLQGEISEKMIDDTCKAANAYDFIQRLPKKYDTIVGEKGALLSGGQKQRIAIARALIRNPSILLLDEASSALDSQSEFIVQSALDKARKGRTTIIVAHR---------------------LSTIRSADAILVMKDGYRVDYGTHESLKSNKTGLYCSLVNAQDCQVDQDEGLPLFNPELNYEEEDEVYDLEQVENEMNSMTYGSISGGSSWNRRHHFVRPTLERRHSTGSG-YSEDSLKIEDALDVAGSAIGIARVGSRK 581
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000007660 (supercontig:LSalAtl2s:LSalAtl2s445:12562:17493:1 gene:EMLSAG00000007660 transcript:EMLSAT00000007660 description:"maker-LSalAtl2s445-augustus-gene-0.18") HSP 1 Score: 253.447 bits (646), Expect = 5.315e-74 Identity = 173/550 (31.45%), Postives = 281/550 (51.09%), Query Frame = 0
Query: 184 MSYVF-VTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLI----FARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGA---GNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVF-LNGIDISKLQLKALR-ERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKE 711
++Y++ + CL E +R S++ QDI ++D ++TG+ SR++ D+ + + I + + +++ ++ ++T + ++P +++ I L+ + KA +V EE +S++RTV F + E+ + + F G+ G+ G F+ ++ + YG L+ DD NL + L+ SV ++ Q + + ++I+ I++ P I ++G + P G+V FK+ VL+ +L I +TVALVG SG GK+T+ L++RFYD + GG ++GI+I L LR IG + QEPVLF ++ +NIRYGR TD ++ A K A+ F++ P Y+T++GERG +SGGQKQRIAIARAL++NP IL+LDEATSALD++SE +VQ ALDK GRT++V+AHR LSTI+ AD I + G +AE+GTH+ L + G+Y L+ Q +E
Sbjct: 171 IAYIYSLACL---GERMAASLRKDLFNSIICQDIAFFDEHKTGEIVSRLSADVQEFKSSFKLVISQGLRSSAQASGCIVSMYMISPQMTSAMGVIVPTVILGGTYIGSYLRVLSKKAQAQVAKATAVGEECISNIRTVRGFAMEDAEMELYSREVDKTRDFNEALGLGIGVFQGASNFFLNSIVLGT----ITYGGYLMSDD------NL-------NPGQLMSFLVSVQTIQRSITQVSLLFGHLVKGMASGSRIFEYIEKVPLIP---ISGGVKIPYHSFFGDVEFKNITFSYPTRPEQAVLKDFSLRIPPSKTVALVGTSGGGKTTIAALLERFYDINGGGSLEIDGINIRDLDPSWLRGSAIGYINQEPVLFATSVIENIRYGRPNATDNEVYEAAKAAHVDDFVRTFPDGYSTILGERGVTVSGGQKQRIAIARALLKNPPILILDEATSALDAESERIVQEALDKLSKGRTSLVIAHR---------------------LSTIKNADVIAVIDKGVMAEIGTHAALKRKGGIYSRLIEQQEFRE 676
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000004490 (supercontig:LSalAtl2s:LSalAtl2s2344:77714:79426:-1 gene:EMLSAG00000004490 transcript:EMLSAT00000004490 description:"maker-LSalAtl2s2344-augustus-gene-0.12") HSP 1 Score: 188.348 bits (477), Expect = 3.144e-52 Identity = 145/435 (33.33%), Postives = 223/435 (51.26%), Query Frame = 0
Query: 202 RIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELK-AYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGM-------LTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG-ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEAT 615
RIR S+ +Q+ ++DTN+TG+ +R++ D V + + +I + + + +A +L LV SV+P V A+ G + + E + A +A+E ++++RTV AFG + +E ++ + G+ + +TG G + L + +YG L+ D NL + +S +G G ++ Y E A GAA +I+ ++DRT + D LP P G++TF+ V GL L+I + +A+VG KST+ L+ R YDP+ G V ++G DI +L LR +IGIV QEPVLF TI +NI YG EG I ++I A KE+NA+ FI P +Y+TLVGERG +SGGQKQR+AIA A+++NP L+LDEAT
Sbjct: 68 RIRSRLFSSITKQETAFFDTNKTGELVNRLSSDSMLVSQALTSQISSGMRSSIMALAGGGMMLFMSPQLALVGLSVVPP-VAGWAVWMGKKVKNISHEYQNTLADATHLAQERIANIRTVRAFGKEVQESMAYDEKMSLVLDKGVKEALIQAKFYGMTGXTGNLIILSV-------MFYGGFLVTQDVITV-GNLTS-----------FILYSGYVGIGLNGVSSFYAEIMK-ALGAATRIWEIMDRTTHM-PLDTGLILPMPLNGHITFEKVGFSYPSRPDHSIFDGLNLNIESKQILAIVGS----KSTLTSLLLRLYDPNMGRVCIDGTDIRELNTTWLRNQIGIVMQEPVLFSGTIKENILYGTEGKIEHEEIVSAAKESNAHDFIMNFPDRYDTLVGERGVLLSGGQKQRVAIAXAILKNPQXLILDEAT 476
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000003104 (supercontig:LSalAtl2s:LSalAtl2s174:364234:370462:-1 gene:EMLSAG00000003104 transcript:EMLSAT00000003104 description:"maker-LSalAtl2s174-augustus-gene-3.18") HSP 1 Score: 187.193 bits (474), Expect = 2.102e-49 Identity = 95/232 (40.95%), Postives = 146/232 (62.93%), Query Frame = 0
Query: 464 KDVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMN 695
K++ GL+ S+ G+T+A+VG SG GKST+++L+ RFY+P G + + +I+++ L +LR +I IV Q+ VLF TI NI+YG +D+D+ K A + I+ P+ Y T VGERG ++SGG+KQR+AIARA +++ I++ DEATS+LDS +E ++ AL + G+T I++AHR LST+ AD+I + +G+V E GTH EL+N
Sbjct: 377 KNIANGLSFSVESGKTIAIVGESGSGKSTLVRLLYRFYEPQLGSIKIGNYNINEVTLDSLRRQISIVPQDCVLFNDTIFHNIKYGNLNCSDEDVYKVAKLAEIDNAIRSWPQGYLTQVGERGLKLSGGEKQRVAIARAALKDSPIIIFDEATSSLDSITETMIMKALKRVTSGKTAIIIAHR---------------------LSTVVHADEIFVLSNGKVIERGTHEELLN 587
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000002221 (supercontig:LSalAtl2s:LSalAtl2s1420:21426:31801:1 gene:EMLSAG00000002221 transcript:EMLSAT00000002221 description:"maker-LSalAtl2s1420-augustus-gene-0.12") HSP 1 Score: 142.895 bits (359), Expect = 6.163e-35 Identity = 136/581 (23.41%), Postives = 244/581 (42.00%), Query Frame = 0
Query: 170 FAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYL------TARELKAYGKAG-----SVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAA------------------------QIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD-----------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSL 703
+ G ++ ++ FV ++++ C+ A R+ L+S+L+ + ++DT G +R+ +DL+ + M + + I+ F+ +V+ PV +I ++I ++ T+R+LK + S E +S T+ A+G S + E + +KA + + W V+L + NL +VF + L+ + +P + S++ A Q Y+ ++ D D P G ++FK+ VL+ ++L I GE + +VG +G GKS+ + R +P G + ++G+DI+KL L LR RI I+ Q+PVLF ++ N+ E + +I A ++ FI L V E G +S GQ+Q I +ARAL+R +L+LDEAT+A+D +++ ++Q + T I +AHR L+TI KI+ +K+G E GT +EL+ ++K +YS+
Sbjct: 1049 YCLGQGIVVVMGFV--FMYLACMEAAQ-----RLHNQMLESILKSPMSFFDTTPQGRILNRLGKDLDVLDSVM-----------PMVLRGWISCFLGVLSSLIVVMVTTPVFIIPASLIIICYFFIQRIYVATSRQLKRLESSSRSPIXSFFSETVSGAATIRAYGQSSTFISESESKVDDNQKAN------------------FPATVSNRWLAVRL------EVVGNL-------------IVFTAALLAVLGRDSLSPGLVGLSVSYALAVTASLNWLVRMASEVETNIVAVERIQEYTKAEKEAPWIIEDTKPDEDWPRKGEISFKNYSTRYREGLDYVLKNVSLDIEGGEKIGIVGRTGAGKSSFTLSMFRIIEPVTGNIIIDGVDITKLGLHQLRSRITIIPQDPVLFSGSLRRNLDPLDE-YNETNIMEAISHSHLKPFIDSLKDGLEYHVSEGGENLSLGQRQLICLARALLRRTRVLILDEATAAVDLETDNLIQKTIRSEFKDSTVITIAHR---------------------LNTIMDYSKILVLKNGERVEYGTVAELLADKKSQFYSM 1552 HSP 2 Score: 99.3673 bits (246), Expect = 1.801e-21 Identity = 74/249 (29.72%), Postives = 120/249 (48.19%), Query Frame = 0
Query: 464 KDVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAY-SFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHG----RTTIVVAH--RWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVM 705
K++L ++L I G VA+VG G GKS+++ + D +G L +R I Q+ + ++ +NI + + DK + EA A S + LP T +GE+G +SGGQKQR+++ARA+ DI LLD+ SA+DS + + + G +T ++V H ++PF DKI+ +KDGRV+E+GT+ EL+ QKG + ++
Sbjct: 651 KNILSDVSLDIEVGSLVAVVGTVGSGKSSLLSAILGEMDKVSG-------------LITVRGSIAYGAQQAWVLNTSLKNNILFNKSYDGDKYTRIV--EACALKSDLDMLPGGDETEIGEKGINLSGGQKQRVSLARAVYSGSDIYLLDDPLSAVDSHVGKHIYDNVISSSSGILKDKTRVMVTHGVTYLPF-----------------------TDKIIVMKDGRVSEIGTYKELLRQKGAFAEFIV 861
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000008248 (supercontig:LSalAtl2s:LSalAtl2s498:229862:245360:1 gene:EMLSAG00000008248 transcript:EMLSAT00000008248 description:"maker-LSalAtl2s498-snap-gene-2.25") HSP 1 Score: 132.109 bits (331), Expect = 1.346e-31 Identity = 78/240 (32.50%), Postives = 132/240 (55.00%), Query Frame = 0
Query: 466 VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSLV 704
VL+G+ +I KGE + +VG +G GKS++ + R +P G ++++G +I L L LR RI I+ Q+P+LF ++ N+ E D+DI +A + ++ SF+ L N V E G +S GQ+Q I +ARA++R IL+LDEAT+A+D +++ ++QS + T + +AHR ++TI +++I+ + G +AE L+ N ++YSLV
Sbjct: 1296 VLKGINCTIQKGEKIGIVGRTGAGKSSLTLALFRIIEPSEGSIYIDGENIRFLGLGKLRSRITIIPQDPILFSGSLRMNLD-PFEAFADRDIWIALEYSHLKSFVWNLGDGLNFSVLEGGRNLSVGQRQLICLARAILRKTQILVLDEATAAIDLETDDLIQSTIRSEFKDSTVLTIAHR---------------------INTIMDSNRIMVLDAGTIAEFDDPQNLLANPDSMFYSLV 1513 HSP 2 Score: 81.6481 bits (200), Expect = 5.984e-16 Identity = 83/310 (26.77%), Postives = 141/310 (45.48%), Query Frame = 0
Query: 465 DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSF-IQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQS-----EAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQSERRPSVASSVFSDDSFTLEDV 768
+ L+ + L I KG VA+VG G GKS+++ + + G + ++G I Q+P L T+ +NI +G+ +K + +A A S + LP T +GERG +SGGQK R+++AR N DI +LD+ SA+D E V+ S ++ +T I V H + P DKI+ +K+G ++E GT+ +L+ ++ ++ + +E E+ + QDL+T+ +++ T G + E+R A SD S L D+
Sbjct: 633 NTLEDIDLDIEKGSLVAIVGQVGSGKSSLLSGILGEMEIVRGSINVDGQTIY-------------APQQPWLQNETLRNNILFGKR--FNKKLYRRVIDACALSPDLDMLPAGDLTEIGERGINLSGGQKARVSLARCTXNNGDIYILDDPLSAVDVHVGRHLFENVISSQTGLLKN-KTRIFVTHGAIFLPQ---------------------TDKIIVMKNGVISESGTYRDLIEKENGEFAQFLINYLVDEK---ENILKNDQDLKTIIKDLE--STVG--GSEELEKRLEAAQ---SDKSTALTDL 895
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000003611 (supercontig:LSalAtl2s:LSalAtl2s197:49356:53594:-1 gene:EMLSAG00000003611 transcript:EMLSAT00000003611 description:"augustus_masked-LSalAtl2s197-processed-gene-0.4") HSP 1 Score: 124.02 bits (310), Expect = 4.278e-29 Identity = 83/263 (31.56%), Postives = 131/263 (49.81%), Query Frame = 0
Query: 456 PGAGNVTFKD-----------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSEL-MNQKGVYYSLVMA 706
P G V FK+ VL+ + +I GE V +VG +G GKS++ + R + G + ++G DIS L L LRE + ++ QEP+LF T+ N+ +D DI + +I+ L N + E G +S GQ+Q + +ARAL+R+ IL+LDEAT+A+D +++ +Q + T + +AHR L TI DKI+ + G V E G+ +EL MN V++S++ A
Sbjct: 1154 PSQGKVEFKNYSLRYKANSDLVLKKINFTIFGGERVGIVGRTGAGKSSLSVALFRIVEAANGQILIDGKDISTLGLHDLRESLTVIPQEPLLFSGTLRSNLD-PENAFSDHDIWNTLGSVHLKEYIRDLKDGLNHSISEGGLNLSSGQRQLMCLARALLRSCKILILDEATAAVDWETDEKIQETIRCYFPSCTILTIAHR---------------------LKTIIDYDKILVLDKGTVIEFGSPNELYMNSDSVFHSMINA 1394 HSP 2 Score: 82.4185 bits (202), Expect = 2.906e-16 Identity = 118/524 (22.52%), Postives = 222/524 (42.37%), Query Frame = 0
Query: 202 RIRGLFLKSVLRQDIGWYDTNQ----TGDFASRMTEDLNKVQEGMGEKIGMFIFFATIF--VASLINAFVH-GWEL---TLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMR--GMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLI-------VVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTP-AIDSSDMNGDLPAPGAGNVTF-----KDVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGR---EGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQK 697
RIR V R+ + ++++ G+ + M+ D++++ + M + + +++ F V S+I + GW + T ++ +PV AI++ +++KA + EVL +R + + + + + E I + GI++ LT G F+W ALA + +++DD N+ + + S I F V++ A++ AT ++ + + ++R P I+ GD GN + ++ L + L I G A+VG G GKS++I + +G V + G +I V QE + ++ +NI +G+ E ++ +E C + + LP T +GERG +SGGQ+QRI++ARA+ + D+ + D SALDSQ V +S + + T I+ T +++ +S + I + G + GT+ EL+ +K
Sbjct: 300 RIRASLTSLVYRKCLKLSNSSRKNKSVGEIVNIMSVDVSRIADLMP---FLTLLWSSPFQIVVSVIFMYNELGWAIIGGTGILLITIPV----NAILSYFGKKFQMKQMKAKDNRTKILNEVLGGIRILKLYAWEPSYINKIEG--IRGVEIGILKKAAWLTSFMG-FIWSSTPFIVALAS-FATFVMVDD-----RNILTAEKAFVTQSYINKMKMPMAXFPFVIVXAIS---ATVSLKRINQLLTSDELQKDAVERVPMKINQKIDQGDAIVIRDGNFKWSTEDSENTLSNINLRIKHGSLTAIVGTVGSGKSSLISAILGEMVKKSGKVRIKG-------------QIAYVPQESWMQNTSLKNNILFGKPYDEEWYNQVLEYCCLTYD----LSLLPAGDETEIGERGINLSGGQRQRISLARAIYSDADVYIFDNPLSALDSQ---VGKSVFNNVLNNNTGIIKD--------------KTRLLVTHGISYLPHVHHIAVMLKGEIIHEGTYQELLEKK 770
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000011700 (supercontig:LSalAtl2s:LSalAtl2s812:79332:86643:-1 gene:EMLSAG00000011700 transcript:EMLSAT00000011700 description:"maker-LSalAtl2s812-augustus-gene-0.6") HSP 1 Score: 120.553 bits (301), Expect = 5.362e-28 Identity = 124/537 (23.09%), Postives = 235/537 (43.76%), Query Frame = 0
Query: 203 IRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGM-----GEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAE--------EVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAG---IMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDT---EQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD-----------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIR-YGREGITDKDIEMACKEANAYSFIQGLPKK--YNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSLVM 705
+ G + +++ + ++DT G +R +D+ + M G G F + AT+ + S+ + + I + + V+ I+ S + ++R+LK + SVA E LS + TV A+ + FE L +K IM G+ + I + AL ++ DT Q N++ S ++ +S L + A I+ +S A P D+ + P G +T K+ VL+ + + I+ G+ + +VG +G GKST+ + R + ++G + ++G+DI K+ L LR ++ I+ Q+P LF T+ N+ +G KD+E+ E+ ++++Q K+ + ++ E G +S GQ+Q I +ARAL+R +L++DEAT+A+DS+++ ++Q A+ T + +AHR L T+ KI+ + G + E + L+ + +Y + +
Sbjct: 1505 LHGTLFQCIIKTPMSFFDTTPLGRILNRFGQDIEVLDTKMSFCIFGSMTGTFAYIATVTIISI--------NVPIFIIPTLAIFVVYFVILIVSLS--SSRQLK---RLSSVATSPIYSHFGETLSGINTVRAYSLEKAFYSEFERRLDEYQKRNFPVIMADRWLGIRINLIGNFIIFASAL-----FSVLSRDTITPGQLGLNVSSSLAITSILSWVIRMYSDLENNI---VAVERIKEYSNKESEA----------PWELEEDLKLNSSWPLKGEITIKNLSLKYRKDLDWVLRDINVHISPGDKIGIVGRTGSGKSTLALALFRLLEVNSGQITIDGVDIKKMGLHTLRSKLTIIPQDPTLFSGTLRLNLDPFG----LFKDMELI--ESLKHAYLQDFLKEDGLDKVIAENGENISLGQRQLICLARALLRKNKVLIMDEATAAVDSETDKLIQKAIKDNFRDSTVLTIAHR---------------------LQTVLDYSKIIVIDSGCIIEYDSPKNLLSDSSSTFYKMCL 1983 HSP 2 Score: 98.2117 bits (243), Expect = 5.053e-21 Identity = 90/343 (26.24%), Postives = 153/343 (44.61%), Query Frame = 0
Query: 463 FKDVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQS-----EAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLV--MAQTKKEETRDGEDEIDEI------QDLET----VDQEISEMGTAGPRNRSQSERRPSVASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQKN 788
FKD+ ++ +N+G +A++GP G GKS++I + G + + +++ + Q + ++ DNI +GR+ + K + S ++ P +T +GE+G +SGGQKQRI++ARA+ DI LD+ + +DS E V+ S + G+T I+V H +LS + D+I +KDG + E GT+ ELM + + L+ A K D E +++I Q +ET D+E G G R + + + + + F + + A GS+V LS V SD + N
Sbjct: 1164 FKDI----SMDVNRGNLIAIIGPVGSGKSSLISAL-------LGEMLFTSKGTNPVEIHG---SVAYTPQNAWMQNVSVKDNILFGRK-FNKNWYDEVLKACSLESDMEXFPSGDSTEIGEKGINLSGGQKQRISLARAVYSQSDIYFLDDPLAXVDSHVAKHLFEEVIGS--NGLLRGKTRILVTH---------------------NLSFLHLVDEIYFLKDGEITEKGTYKELMEKNCDFSELISNHANNKITNQSDSEKVMEKINEEGTKQPIETNFKIYDEETLHTGQVGIRVYWYFLSKIGMLAGILAFSFFIISQI-LATGSSVWLS-VWSDAKNN 1466
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000002016 (supercontig:LSalAtl2s:LSalAtl2s1361:43077:48755:-1 gene:EMLSAG00000002016 transcript:EMLSAT00000002016 description:"augustus_masked-LSalAtl2s1361-processed-gene-0.2") HSP 1 Score: 112.464 bits (280), Expect = 1.639e-25 Identity = 99/376 (26.33%), Postives = 165/376 (43.88%), Query Frame = 0
Query: 336 NLIFARKAG-IMRGMLTGVGGGFMWLV-IYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLP-------APGAGNVTFKDVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYS 702
NL F RK + G+ + LV IY ++ + F ++ M+ E+ E D S F +V +MN + + + RG +I + + +D G + AP + QG +L I + V + G SG GKST++ + R G + +NGI+I+ + L LR+ + + Q+ VLF TI N+ +D+ I +A+ ++ P +T V E G S GQ+Q + I +AL+R P +++LDE+TSALD E + + L +A T I VAHR LS I ++++ + +GR+ E G EL+ + ++S
Sbjct: 1535 NLFFPRKGREVTSSASIGLSVNYSLLVPIYLAWVVKFAANIENYMNAVERVLEYTHFPSEEEDFSE----FQAVHASSMNSSRRSFLLRG----RGKHDEIEEDVRVSGDFLQNDSEGLIIRFNSVCLAPSFEYRRLPGIQQGFSLEIPYRQKVGICGRSGSGKSTLLMGIVRLSRVLQGSITINGININAIPLSRLRKFVITIPQDAVLFSGTIRSNLD-PENDFSDELIWSTLDKADCGKTVRNFPDGLDTCVTENGDNFSLGQRQELNIVKALLRRPRVVILDESTSALDPNREVALHNTLLEAFEDSTLISVAHR---------------------LSNIVEYERVLVMGEGRILEDGNPKELLKKPMGFFS 1880 HSP 2 Score: 96.2857 bits (238), Expect = 1.922e-20 Identity = 84/305 (27.54%), Postives = 136/305 (44.59%), Query Frame = 0
Query: 465 DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRD---GEDEIDEIQDLETVDQEISEMGTAGPRNRSQSERRPSVASSVFSDDSFTLE 766
+VLQ + L I G ++GPSG GK+ +I + D G V N D + ++GQ P L TI DNI GR +K + + + I LP +T +GERG +SGGQ+QR+AIAR L +D S+LDS+ + V G I++ R F + +R L + AD IVA+KDG + GT ++ S +++ + D G E Q+ + + ++++G +N++ +++ + + SDD+F LE
Sbjct: 743 NVLQSINLEIKTGSLTIVIGPSGSGKTALISSLIEEMDRITGSVKWNVPDT-----------VALLGQRPWLLNTTIKDNILLGRP-FKEKRYKKVIAACDLQTDIDLLPHGDDTEIGERGVLLSGGQRQRLAIARCLYSKSYCTFMDAPFSSLDSKITSHV------FEEGVLKILLKRRRTVFLTTER------------LDFLHRADHIVALKDGMIKAQGTIQDVTRLCPELLSTKLSKNRLSRISDDNKGLVEGKTAQERWKLLRNVTKLGIK-VKNKNTPKKKSQLQLNK-SDDNFPLE 1015
BLAST of P-glycoprotein vs. L. salmonis genes
Match: EMLSAG00000000752 (supercontig:LSalAtl2s:LSalAtl2s111:55855:93761:1 gene:EMLSAG00000000752 transcript:EMLSAT00000000752 description:"maker-LSalAtl2s111-augustus-gene-1.10") HSP 1 Score: 109.383 bits (272), Expect = 1.435e-24 Identity = 64/197 (32.49%), Postives = 110/197 (55.84%), Query Frame = 0
Query: 447 SDMNGDLPAPGAGNVTFKDV-----------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDK 632
SD+ D P G V F +V L+GL N E + ++G +G GKS++I + R +P G +F++G+DI K+ L +R++I I+ Q+P+LF +I N+ E TD I A ++A Y + L +++V E G+ S GQ+Q +A A++R +L++DEAT+ +D +++++Q A+ K
Sbjct: 36 SDIKLDPSWPDKGVVEFSNVSMRYSQHQPNVLKGLNFKTNSFEKIGIIGRTGAGKSSIISALFRLAEPE-GEIFIDGLDICKVGLLDIRKKISIIPQDPILFNGSIRKNLDPFNE-FTDVQIWNALEQAKLYDIVVDLGHGLDSVVLEFGSNFSVGQRQLFCLAXAILRRNKVLIMDEATANVDPFTDSLIQEAIRK 230 HSP 2 Score: 96.2857 bits (238), Expect = 1.750e-20 Identity = 77/285 (27.02%), Postives = 129/285 (45.26%), Query Frame = 0
Query: 467 LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQ-SEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVK-DGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQSER 749
L+ ++ S+ GE A++GP GCGKST+IQ + + G + + G RI QE +F ++ NI G+ + DK + ++ P T VGE+G +SGGQK RI +AR + + DI +LD+ SA+D + + + + K +T I+V H+ L ++ A+ I+ + DG + GT+ +LM+Q G T E T + ED D + D+ +S++ + G RSQ ++
Sbjct: 734 LKNISFSVEIGELCAIIGPVGCGKSTIIQSLLGEFPVSYGEINIKG-------------RISYASQEAWIFSGSVRQNILMGKP-LIDKRYREVIRVCALEHDVREWPDGDYTFVGEKGISLSGGQKSRINLARCVYASADIYILDDPLSAVDPHVGQQLFDTCIQKFLSDKTVILVTHQ---------------------LQFLKNANNIILLNSDGEIEASGTYEKLMSQSGFV-------TYLEATSEEEDS-DLLSDV------LSDVKSDGEYMRSQKKK 969 HSP 3 Score: 92.8189 bits (229), Expect = 1.964e-19 Identity = 57/204 (27.94%), Postives = 103/204 (50.49%), Query Frame = 0
Query: 506 GGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNI----RYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSLV 704
G +F++G++I +L L+ +R++I I+ Q+P+LF TI N+ YG I A ++A + L T V E G S GQ+Q +ARA++R +L++DEAT+ +D +++++Q A+ T +AHR L T+ +D+I+ + DG + E+G +L+ + ++Y +V
Sbjct: 1399 GKIFIDGVNICRLSLQDVRKKISIIPQDPLLFKGTIRKNLDPFDEYGELAIWH-----ALEQAKLSVAVSELEGGLETEVSEGGNNFSVGQRQLFCLARAILRKNKVLIMDEATANVDPMTDSLIQEAIRTEFKDCTVFTIAHR---------------------LYTVMDSDRILVMSDGEIKELGAPLDLLSDPSNIFYQMV 1576
BLAST of P-glycoprotein vs. SwissProt
Match: gi|238054374|sp|P08183.3|MDR1_HUMAN (RecName: Full=Multidrug resistance protein 1; AltName: Full=ATP-binding cassette sub-family B member 1; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243) HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0 Identity = 315/697 (45.19%), Postives = 426/697 (61.12%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRN---RSQSERR 750
P VSVF +FR+++ D + + LAA+I G +PLM+++FG++ + F N N+ + + D D G F+ N E +TR+A + IG V +Y+ V+ AA Q+++IR F +++RQ+IGW+D + G+ +R+T+D++K+ EG+G+KIGMF F I F GW+LTLVI ++ PVL +S A+ A + T +EL AY KAG+VAEEVL+++RTV+AFGGQ KE+ R+ NL A++ GI + + + G +L+IY SYALAFWYG L++ Y ++ VFFSVL+GA +VGQA+P IEAF+ ARGAA +I+ +ID P+IDS +G P GN+ F++V L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ ALDKAR GRTTIV+AHR LST+R AD I DG + E G H ELM +KG+Y+ LV QT E E++ D + + EM + R+ R +S RR
Sbjct: 32 PTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFA-NAGNLEDLMSNITNRSDI---------------NDTGFFM---NLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSG-------------EYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHR---------------------LSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEV-----ELENAADESKSEIDALEMSSNDSRSSLIRKRSTRR 670 HSP 2 Score: 397.512 bits (1020), Expect = 5.719e-121 Identity = 252/731 (34.47%), Postives = 394/731 (53.90%), Query Frame = 0
Query: 4 LEDLAEEPESFNSNSSCPSLDIGSH--KSPRLASDTVPWTENGSNSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAI---IAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ--TKKE 711
LE+ A+E S S +L++ S+ +S + + + GS + +D +E + E S+PPVS +++ + + + + + A+I G P I+F + F + DD E + N+N F + +G+++F+ ++ A E R+R + +S+LRQD+ W+D N TG +R+ D +V+ +G ++ + +I +F++GW+LTL++ +++P++ I+ + + Q +EL+ GK +A E + + RTVV+ + K + +L + + + + G+ F ++Y SYA F +G L+ +E++ ++VF +V+ GAM VGQ + + ++ A+ +AA I +I++TP IDS G +P GNVTF +V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG V L+G +I +L ++ LR +GIV QEP+LF C+IA+NI YG ++ ++I A KEAN ++FI+ LP KY+T VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV ++GRV E GTH +L+ QKG+Y+S+V Q TK++
Sbjct: 637 LENAADE-----SKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQA-----QDRKLSTKEALDE-SIPPVSFWRIMKLNLTEWPYFV-VGVFCAIINGGLQPAFAIIFSKIIGVFTR---------------IDD----------PETKRQ-------NSNLFSLLF------LALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGK---IATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKL-MSFEDV------------LLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ 1280
BLAST of P-glycoprotein vs. SwissProt
Match: gi|126924|sp|P21448.2|MDR1_CRIGR (RecName: Full=Multidrug resistance protein 1; AltName: Full=ATP-binding cassette sub-family B member 1; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243) HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0 Identity = 303/674 (44.96%), Postives = 417/674 (61.87%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVD 730
P VSVF +FR+A D + + LAA+I G ++PLM+++FGD+ ++F +P + +V DI E++T +A IG +++Y+ V+ AA Q+++IR F +++ Q+IGW+D + G+ +R+T+D++K+ EG+G+KIGMF F I F GW+LTLVI ++ PVL +S I A + T +EL+AY KAG+VAEEVL+++RTV+AFGGQ KE+ R+ +NL A++ GI + + + G +L+IY SYALAFWYG L++ Y ++ VFF+VL+GA ++GQA+P IEAF+ ARGAA +I+++ID P+IDS NG P GN+ FK++ L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ+ALDKAR GRTTIV+AHR LST+R AD I G + E G H ELM +KG+Y+ LVM QT E G + + +++ +D
Sbjct: 31 PVVSVFTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLVFGDMTDSFAS--------VGNIPTNATN--------NATQVNASDI-----FGKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISK-------------EYSIGQVLTVFFAVLIGAFSIGQASPNIEAFANARGAAYEIFNIIDNKPSIDSFSKNGYKPDNIKGNLEFKNIHFSYPSRKDVQILKGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHR---------------------LSTVRNADIIAGFDGGVIVEQGNHEELMREKGIYFKLVMTQTAGNEIELGNEVGESKNEIDNLD 649 HSP 2 Score: 404.06 bits (1037), Expect = 1.713e-123 Identity = 237/675 (35.11%), Postives = 368/675 (54.52%), Query Frame = 0
Query: 52 DKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDT--NQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAI---IAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
D+D +E + E +PP+S +++ + S + + + + A++ G P I+F + F +N DD E D +N F + ++G+++F+ ++ A E R+R + KS+LRQD+ W+D N TG +R+ D +V+ G ++ + +I + ++GW+LTL++ +++P++ I+ + + Q +EL+ GK +A E + + RTVV+ + K + +L + + + + G+ F ++Y SYA F +G L+ + +EN+ ++VF +++ GAM VGQ + + ++ A+ +A+ I +I++ P+IDS G P GNV F +V LQGL L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG VFL+G ++++L ++ LR +GIV QEP+LF C+IA+NI YG ++ +IE A KEAN + FI+ LP KYNT VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V Q
Sbjct: 674 DQDRKLSTKEALDE-DVPPISFWRILKLNSSEWPYFV-VGIFCAIVNGALQPAFSIIFSKVVGVFTRN--------------TDD-----------ETKRHD-------SNLFSLLF------LILGVISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDNPKNTTGALTTRLANDAGQVKGATGARLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGK---IATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAREL-MTFENV------------LLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMIIEKVPSIDSYSTGGLKPNTLEGNVKFNEVVFNYPTRPDIPVLQGLNLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEVNQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1271
BLAST of P-glycoprotein vs. SwissProt
Match: gi|239938877|sp|P21447.3|MDR1A_MOUSE (RecName: Full=Multidrug resistance protein 1A; AltName: Full=ATP-binding cassette sub-family B member 1A; AltName: Full=MDR1A; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3) HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0 Identity = 305/695 (43.88%), Postives = 421/695 (60.58%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFV------QNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRS 745
P VSV +FR+A D + + LAA+I G ++PLM+++FGD+ ++F +N NM++ + F E++T +A IG +++Y+ V+ AA Q+++IR F +++ Q+IGW+D + G+ +R+T+D++K+ EG+G+KIGMF F I F GW+LTLVI ++ PVL +S I A + T +EL AY KAG+VAEEVL+++RTV+AFGGQ KE+ R+ +NL A++ GI + + + G +L+IY SYALAFWYG L++ Y ++ VFFSVL+GA +VGQA+P IEAF+ ARGAA +++ +ID P+IDS +G P GN+ FK++ L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP +++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ+ALDKAR GRTTIV+AHR LST+R AD I G + E G H ELM +KG+Y+ LVM QT E G + +++ +D + G++ R RS
Sbjct: 31 PAVSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFA----------------------------KLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISK-------------EYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTAGNEIELGNEACKSKDEIDNLDMSSKDSGSSLIRRRS 663 HSP 2 Score: 394.045 bits (1011), Expect = 1.018e-119 Identity = 238/677 (35.16%), Postives = 364/677 (53.77%), Query Frame = 0
Query: 52 DKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAI---IAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQ--CYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
D+D +E + E +PP S +++ + S + + + + A+I G P ++F + F TQ N+N F + ++G+++F+ ++ A E R+R + KS+LRQD+ W+D N TG +R+ D +V+ G ++ + +I + ++GW+LTL++ +++P++ I+ + + Q +EL+ GK +A E + + RTVV+ + K + +L + + + + G+ F ++Y SYA F +G L+ T+Q +EN+ ++VF +++ GAM VGQ + + ++ A +A+ I +I++TP IDS G P GNV F V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG VFL+G +I +L ++ LR ++GIV QEP+LF C+IA+NI YG ++ ++I A KEAN + FI LP KYNT VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V Q
Sbjct: 673 DQDRKLSTKEALDE-DVPPASFWRILKLNSTEWPYFV-VGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQ--------------------------------NSNLFSLLF------LILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGK---IATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLV---TQQLMTFENV------------LLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQ 1270
BLAST of P-glycoprotein vs. SwissProt
Match: gi|126927|sp|P06795.1|MDR1B_MOUSE (RecName: Full=Multidrug resistance protein 1B; AltName: Full=ATP-binding cassette sub-family B member 1B; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243) HSP 1 Score: 562.377 bits (1448), Expect = 0.000e+0 Identity = 305/714 (42.72%), Postives = 431/714 (60.36%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGP----------RNRSQSERRPSVASSVFSD 760
P V VF +FR+A D + L LAA+I G +PL++++FG++ ++F + + ++ +P + S P + I N++ E++ +A IG +++Y+ V+ AA Q+++IR F +++ Q+IGW+D + G+ +R+T+D++K+ +G+G+KIGMF T F+A I F+ GW+LTLVI +V P++ +S+A+ A T T +EL+AY KAG+VAEEVL+++RTV+AFGGQ KE+ R+ NL A+ GI + + + G +L++Y SYALAFWYG L++ + Y ++ VFFS+L+G ++G P IEAF+ ARGAA +I+ +ID P+IDS G P GN+ FK+V L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP +++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ+ALDKAR GRTTIV+AHR LST+R AD I G + E G H ELM +KG+Y+ LVM QT+ E G + D + E++ + P + ERR S+ +V D
Sbjct: 31 PAVGVFGMFRYADWLDKLCMILGTLAAIIHGTLLPLLMLVFGNMTDSFTKAEASI------LPSITNQ------SGPNSTL-------IISNSSLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSN-------------EYSIGEVLTVFFSILLGTFSIGHLAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTRGNEIEPGNNAYGSQSDTDA--SELTSEESKSPLIRRSIYRSVHRKQDQERRLSMKEAVDED 689 HSP 2 Score: 399.053 bits (1024), Expect = 1.319e-121 Identity = 242/678 (35.69%), Postives = 365/678 (53.83%), Query Frame = 0
Query: 49 KDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG--REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKK 710
+ D++ +E V E +P VS +++ +L+ + +L A+I GC P+ I+F + F ++D + E ++ F L FF ++GL++FV + A E R+R + KS+LRQDI W+D N TG +R+ D + V+ MG ++ + +I + V+GW+LTL++ ++P++V+ I + ++ K +G +A E + + RT+V+ + K + +L + + + + G+ F ++Y SYA F +G L+ +EN+ ++VF +V+ GAM G + + ++ A+ +A+ I +I++TP IDS G P GNV F V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG VFL+G +I +L ++ LR +GIV QEP+LF C+IA+NI YG ++ ++I A KEAN + FI LP KYNT VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V A K+
Sbjct: 672 RKQDQERRLSMKEAVDE-DVPLVSFWRILNLNLSEWPYLL-VGVLCAVINGCIQPVFAIVFSRIVGVFSRDDDH-------------------------ETKRQNCNLFSL---FF----------LVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAQQL-MTFENV------------MLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVQAGAKR 1275
BLAST of P-glycoprotein vs. SwissProt
Match: gi|1170902|sp|P43245.1|MDR1_RAT (RecName: Full=Multidrug resistance protein 1; AltName: Full=ATP-binding cassette sub-family B member 1; AltName: Full=P-glycoprotein 1; AltName: CD_antigen=CD243) HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0 Identity = 296/674 (43.92%), Postives = 412/674 (61.13%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFV-----QNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQD 725
P V +F +FR+A D + L LAA+I G +PL++++FG + ++F +D +T +E + H + + + E + +A IG +++Y+ V+ AA Q+++IR F +++ Q+IGW+D N G+ +R+T+D++K+ +G+G+K+GMF T F A I F+ GW+LTLVI +V P++ +S+A+ A T T +EL+AY KAG+VAEEVL+++RTV+AFGGQ KE+ R+ NL A++ GI + + + G +L++Y SYALAFWYG L++ + Y ++ VFFS+L+G ++G P IEAF+ ARGAA +I+ +ID P+IDS G P GN+ FK+V L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ+ALDKAR GRTTIV+AHR LST+R AD I G + E G H ELM +KG+Y+ LVM QT+ E G + + D
Sbjct: 30 PAVGIFGMFRYADWLDKLCMALGTLAAIIHGTLLPLLMLVFGYMTDSFTPSRDPHSDRAITN-----------------------QSEINSTHTVSDTSLEEDMAMYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVNDAGELNTRLTDDVSKINDGIGDKLGMFFQSITTFSAGFIIGFISGWKLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRVGIKKAITANISIGIAYLLVYASYALAFWYGTSLVLSN-------------EYSIGQVLTVFFSILLGTFSIGHLAPNIEAFANARGAAYEIFKIIDNEPSIDSFSTKGHKPDSIMGNLEFKNVYFNYPSRSEVKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPIEGEVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYFKLVMTQTRGNEIEPGNNAYESQSD 646 HSP 2 Score: 385.185 bits (988), Expect = 1.748e-116 Identity = 241/682 (35.34%), Postives = 368/682 (53.96%), Query Frame = 0
Query: 52 DKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFI----FFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAI---IAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKK 710
D++ +E V E +P VS +++ + +L+ + +L A+I GC P+ I+F + F ++D + T+ C+ F L L ++G+++FV + A E R+R + KS+LRQDI W+D N TG +R+ D + V+ MG ++ + T + SL+ V+GW+LTL++ ++P++V+ I + Q +EL+ GK +A E + + RTVV+ + K + +L + + + + G+ F +IY SYA F +G L+ + +EN+ ++VF +V+ GAM G + + ++ A+ +A+ I +I++ P IDS G P GNV F V LQGL+ + KG+T+ LVG SGCGKSTV+QL++RFY+P AG VFL+G +I +L ++ +R +GIV QEP+LF C+IA+NI YG ++ ++I A +EAN + FI LP+KYNT VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT +V+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V A K+
Sbjct: 675 DQERRLSSKEDVDE-DVPMVSFWQILKLNISEWPYLV-VGVLCAVINGCIQPVFAIVFSKIVGVFSRDDDHETK-----QRNCNLFSLLFL---------------------------------VMGMISFVTYFFQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDHKNTTGSLTTRLASDASNVKGAMGSRLAVVTQNVANLGTGIILSLV--LVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKELEISGK---IATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNALKKAHVFGITFAFTQAMIYFSYAACFRFGAYLVAREL-MTFENV------------MLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIGIIEKIPEIDSYSTEGLKPNWLEGNVKFNGVKFNYPTRPNIPVLQGLSFEVKKGQTLRLVGSSGCGKSTVVQLLERFYNPMAGTVFLDGKEIKQLNVQCVRA-LGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIVRAAREANIHQFIDSLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCVVIAHR---------------------LSTIQNADLIVVIQNGQVKEHGTHQQLLAQKGIYFSMVQAGAKR 1276
BLAST of P-glycoprotein vs. SwissProt
Match: gi|126928|sp|P21449.2|MDR2_CRIGR (RecName: Full=Multidrug resistance protein 2; AltName: Full=P-glycoprotein 2) HSP 1 Score: 557.37 bits (1435), Expect = 0.000e+0 Identity = 303/707 (42.86%), Postives = 424/707 (59.97%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQS----------ERRPSV 753
P V +F +FR+A D + L LAA++ G S+PL++++FG++ ++F + + ++ P E +++ + E + +A IG +++Y+ V+ AA Q+ +IR F +++ Q+IGW+D + G+ +R+T+D++K+ +G+G+KIGMF F+A+ I F+ GW+LTLVI +V P++ +S+A+ A T T +EL+AY KAG+VAEEVL+++RTV+AFGGQ+KE+ R+ NL A+ GI + + + G +L++Y SYALAFWYG L++ + Y ++ VFFS+L G ++G P IE F+ ARGAA +I+ +ID P+IDS G P GN+ FK+V L+GL L + G+TVALVG SGCGKST +QL+QR YDP G V ++G DI + ++ LRE IG+V QEPVLF TIA+NIRYGRE +T +IE A KEANAY FI LP K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEAVVQ+ALDKAR GRTTIV+AHR LST+R AD I G + E G H ELM +KG+Y LVM QT+ E G E D Q +T+ E++ P R + ERR SV
Sbjct: 32 PNVGIFGMFRYADWLDKLYMVLGTLAAVLHGTSLPLLMLVFGNMTDSFTKAETSI--------------------WPNMTNQSEINNTEVISGSLEEDMATYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQINKIRQKFFHAIMNQEIGWFDVHDIGELNTRLTDDVSKINDGIGDKIGMFFQSIATFLAAFIVGFISGWKLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQNKELERYNKNLEEAKNVGIKKAVTANISIGIAYLLVYASYALAFWYGTSLVLSN-------------EYSVGQVLTVFFSILFGTFSIGHIAPNIEVFANARGAAYEIFKIIDNEPSIDSFSTQGHKPDSVMGNLEFKNVHFSYPSRSGIKILKGLNLKVQSGQTVALVGKSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFDGGVIVEQGNHEELMKEKGIYCRLVMMQTRGNEVELG-SEADGSQS-DTIASELTSEEFKSPSVRKSTCRSICGSQDQERRVSV 682 HSP 2 Score: 394.045 bits (1011), Expect = 9.379e-120 Identity = 248/724 (34.25%), Postives = 379/724 (52.35%), Query Frame = 0
Query: 6 DLAEEPESFNSNSSCPSLDIGSHKSPRLASDTVPWTENGSNSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAI---IAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKK 710
+L E + S++ L KSP + T + + EA++ + +P VS + + + +L+ + +L A+I GC P+ I+F + F ++D T+ +++ F L FF ++G++ FV + A E R+R + KS+LRQDI W+D N TG +R+ D V+ M ++ +I + V+GW+LTL++ + P++++S + + Q +EL+ GK +A E + + RTVV+ + K + +L + + + + G+ F ++Y SYA F +G L+ +EN+ ++VF +V+ GA+ G A+ + ++ A+ +A+ I ++++ P+IDS G P GNV F +V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG VFL+G +I +L ++ LR +GIV QEP+LF C+IA+NI YG ++ +IE A KEAN + FI+ LP KYNT VG++G Q+SGGQKQRIAIARALVR P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V A K+
Sbjct: 635 ELGSEADGSQSDTIASELTSEEFKSPSVRKSTCRSICGSQDQERRVSVKEAQD-------EDVPLVSFWGILKLNITEWPYLV-VGVLCAVINGCMQPVFSIVFSGIIGVFTRDDDPKTK-------------------------QQNCNLFSL---FF----------LVMGMICFVTYFFQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDHRNSTGALTTRLASDAANVKGAMSSRLAGITQNVANLGTGIIISLVYGWQLTLLLVVIAPLIILSGMMEMKVLSGQALKDKKELEVSGK---IATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVAHQI-MTFENV------------MLVFSAVVFGAIAAGNASSFAPDYAKAKVSASHIIRIMEKIPSIDSYSTRGLKPNWLEGNVKFNEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVQAGAKR 1275
BLAST of P-glycoprotein vs. SwissProt
Match: gi|126302568|sp|P21439.2|MDR3_HUMAN (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3) HSP 1 Score: 536.954 bits (1382), Expect = 1.817e-173 Identity = 299/675 (44.30%), Postives = 412/675 (61.04%), Query Frame = 0
Query: 60 QEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDE 722
Q++ K K++ + V LFR++ +D + L + A+ G +PLM+I+FG++ + FV N F L L PG + EE +TR+A + +G V +Y+ V+ AA Q+ +IR F ++LRQ+IGW+D N T + +R+T+D++K+ EG+G+K+GMF F A I F+ GW+LTLVI ++ P+L +S A+ A + + +EL AY KAG+VAEE L ++RTV+AFGGQ+KE+ R++ +L A++ GI + + + G +L+IY SYALAFWYG L++ Y + + VFFS+L+GA +VGQA P I+AF+ ARGAA I+ +ID P IDS G P GN+ F DV L+GL L + G+TVALVG SGCGKST +QL+QR YDP G + ++G DI + LRE IG+V QEPVLF TIA+NI YGR +T +I+ A KEANAY FI LP+K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEA VQ+ALDKAR GRTTIV+AHR LST+R AD I +DG + E G+HSELM ++GVY+ LV QT + + E E+++
Sbjct: 29 QKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGN---------FSFPVNFSLSLLNPGKILEEE--------------MTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISK-------------EYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQTSGSQIQSEEFELND 646 HSP 2 Score: 377.867 bits (969), Expect = 9.452e-114 Identity = 214/559 (38.28%), Postives = 322/559 (57.60%), Query Frame = 0
Query: 176 LIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVIS----TAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVF-------LNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
+G+++F ++ A E R+R + K++LRQD+ W+D N TG ++R+ D +VQ G ++ + +I +F++GW+LTL++ +V+P++ +S ++AG+ R+ K AG +A E + ++RTVV+ + K + L + + + + G+ +Y SYA F +G LI++ + + +I+VF +++ GA+ +G A+ + ++ A+ +AA ++ + +R P IDS G P GN+TF +V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG VF L+G + KL ++ LR ++GIV QEP+LF C+IA+NI YG ++ +I A K AN + FI+ LP KY T VG++G Q+SGGQKQRIAIARAL+R P ILLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV ++GRV E GTH +L+ QKG+Y+S+V Q
Sbjct: 760 FLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGN----AKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRD-------------VILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVFVDFGFQLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQ 1280
BLAST of P-glycoprotein vs. SwissProt
Match: gi|338817954|sp|P21440.2|MDR3_MOUSE (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 2; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 2; AltName: Full=P-glycoprotein 3) HSP 1 Score: 535.413 bits (1378), Expect = 6.045e-173 Identity = 300/674 (44.51%), Postives = 412/674 (61.13%), Query Frame = 0
Query: 60 QEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEID 721
Q + K+K + + + LFR++ +D + L L A+ G +PLM+I+FG++ + FV N N F L + PG + EE +TR+A + +G V +Y+ V+ AA Q+ +IR F ++LRQ++GW+D T + +R+T+D++K+ EG+G+K+GMF F A I F+ GW+LTLVI ++ P+L +STA+ A + + +EL AY KAG+VAEE L ++RTV+AFGGQ+KE+ R++ +L A+K GI + + + G +L+IY SYALAFWYG L++ Y + + VFFS+L+GA +VGQA P I+AF+ ARGAA I+ +ID P IDS G P GN+ F DV L+GL L + G+TVALVG SGCGKST +QL+QR YDP G + ++G DI ++ LRE IG+V QEPVLF TIA+NIRYGR +T +IE A KEANAY FI LP+K++TLVG+RGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEA VQ+ALDKAR GRTTIV+AHR LSTIR AD I +DG + E G+HSELM ++G+Y+ LV QT + E E++
Sbjct: 26 QGREKKKKVNLIGLLTLFRYSDWQDKLFMFLGTLMAIAHGSGLPLMMIVFGEMTDKFVDNTGN---------FSLPVNFSLSMLNPGRILEEE--------------MTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISK-------------EYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHR---------------------LSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQTAGSQILSEEFEVE 642 HSP 2 Score: 382.104 bits (980), Expect = 2.185e-115 Identity = 214/551 (38.84%), Postives = 325/551 (58.98%), Query Frame = 0
Query: 177 IGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVL----VISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
+G+++F ++ A E R+R + K++LRQD+ W+D N TG ++R+ D +VQ G ++ + +I +F++GW+LTL++ SV+P + ++ ++AG+ R+ K AG +A E + ++RTVV+ + K + L + + + + G+ +Y SYA F +G LI++ + + +I+VF ++++GA+ +G A+ + ++ A+ +AA ++S+ +R P IDS G P G+VTF +V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG V L+G + KL ++ LR ++GIV QEP+LF C+IA+NI YG + +I A KEAN + FI+ LP+KYNT VG++G Q+SGGQKQRIAIARAL+R P +LLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V Q
Sbjct: 758 LGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGN----AKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKD-------------VILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIENGKVKEHGTHQQLLAQKGIYFSMVNIQ 1270
BLAST of P-glycoprotein vs. SwissProt
Match: gi|126930|sp|P23174.1|MDR3_CRIGR (RecName: Full=Phosphatidylcholine translocator ABCB4; AltName: Full=ATP-binding cassette sub-family B member 4; AltName: Full=Multidrug resistance protein 3; AltName: Full=P-glycoprotein 3) HSP 1 Score: 531.561 bits (1368), Expect = 1.915e-171 Identity = 297/674 (44.07%), Postives = 411/674 (60.98%), Query Frame = 0
Query: 60 QEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEID 721
Q + K+K + + LFR++ +D + L + A+ G +PLM+I+FG++ + FV N N F L + PG + EE +TR+A + +G V +Y+ V+ AA Q+ +IR F ++LRQ++GW+D T + +R+T+D++K+ EG+G+K+GMF F A I F+ GW+LTLVI ++ P+L +S A+ A + + +EL AY KAG+VAEE L ++RTV+AFGGQ+KE+ R++ +L A+K GI + + + G +L+IY SYALAFWYG L++ Y + + VFFS+L+GA +VGQA P I+AF+ ARGAA I+ +ID P IDS G P GN+ F DV L+GL L + G+TVALVG SGCGK+T +QL+QR YDP G + ++G DI ++ LRE IG+V QEPVLF TIA+NIRYGR +T ++I+ A KEANAY FI LP+K++TLVGERGAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD++SEA VQ+ALDKAR GRTTIV+AHR LST+R AD I +DG + E G+HSELM ++GVY+ LV QT + E E++
Sbjct: 29 QGRNKKKKVNLIGPLTLFRYSDWQDKLFMLLGTIMAIAHGSGLPLMMIVFGEMTDKFVNNAGN---------FSLPVNFSLSMINPGRILEEE--------------MTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQNFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISK-------------EYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLDFSDVHFSYPSRANIKILKGLNLKVQSGQTVALVGNSGCGKTTTLQLLQRLYDPTEGTISIDGQDIRNFNVRYLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMEEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHR---------------------LSTVRNADVIAGFEDGVIVEQGSHSELMQKEGVYFKLVNMQTSGSQILSQEFEVE 645 HSP 2 Score: 377.481 bits (968), Expect = 1.184e-113 Identity = 213/551 (38.66%), Postives = 322/551 (58.44%), Query Frame = 0
Query: 177 IGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVIS----TAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG--ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
+G+++F ++ A E R+R + K++LRQD+ W+D N TG ++R+ D +VQ G ++ + +I +F++GW+LTL++ SV+P + +S ++AG+ R+ KA AG +A E + ++RTVV+ + K + L + + + G+ +Y SYA F +G LI++ + + +I+VF +++ GA+ +G A+ + ++ A+ +AA ++S+ +R P IDS G P G+VTF +V LQGL+L + KG+T+ALVG SGCGKSTV+QL++RFYDP AG V L+G + KL ++ LR ++GIV QEPVLF C+IA+NI YG ++ +I A K AN + FI+ LP+KY T VG++G Q+SGGQKQR+AI RAL+R P +LLLDEATSALD++SE VVQ ALDKAR GRT IV+AHR LSTI+ AD IV +++G+V E GTH +L+ QKG+Y+S+V Q
Sbjct: 763 LGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDYKNSTGALSTRLATDRAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVSGIVEMKMLAGN----AKRDKKALEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHEPYRNSVQMAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRD-------------VILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANMPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNIQWLRAQLGIVSQEPVLFDCSIAENIAYGDNSRVVSQDEIVRAAKAANIHPFIETLPQKYKTRVGDKGTQLSGGQKQRLAIRRALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHR---------------------LSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVNIQ 1275
BLAST of P-glycoprotein vs. SwissProt
Match: gi|296436437|sp|Q00449.2|MDR49_DROME (RecName: Full=Multidrug resistance protein homolog 49; AltName: Full=P-glycoprotein 49) HSP 1 Score: 531.176 bits (1367), Expect = 4.521e-171 Identity = 293/665 (44.06%), Postives = 405/665 (60.90%), Query Frame = 0
Query: 72 SVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVT----EEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFK------------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEI 720
S F LFR+++ + FL+ ++LL A +P +I++G+ + V + GV F L + G ++T EE+ N + T F G+ + + F++ + + N A Q+ RIR LFL+++LRQDI WYDT+ +FAS+MTEDL+K++EG+GEKI + +F FV +++AFV+GW+LTLV+ S +P ++ +T+++A Q L +ELK+Y A +V EEV S +RTV AF GQ KE RF LI A G +G+ +G+G WL+IY ALA WYGV LI+D+ + D Y + L++V F+V+MGA N+G A+P++EA ++A A ++++IDR +D D G+ P AG++ F+ ++L+GLT+ + G+TVA VG SGCGKST+IQL+QRFYDP AG V L+G D+ L + LR +IG+VGQEPVLF TI +NIRYGR T DIE A + AN + FI LPK Y+T VGE+GAQ+SGGQKQRIAIARALVR P +LLLDEATSALD SE VQSAL+ A G TT+VVAHR LSTI ADKIV +KDG VAE GTH ELM ++G+Y LV +KE T E +
Sbjct: 30 SYFDLFRYSTRCERFLLVVSLLVATAASAFIPYFMIIYGEFTSLLVDRTV-------GVGTSSPAF-ALPMFGGGQQLTNASKEEN------NQAIIDDATAFGIGSLVGSVAMFLLITLAIDLANRIALNQIDRIRKLFLEAMLRQDIAWYDTSSGSNFASKMTEDLDKLKEGIGEKIVIVVFLIMTFVIGIVSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKELKSYSDAANVVEEVFSGIRTVFAFSGQEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYLCMALAIWYGVTLILDERDLP-------DRVYTPAVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFNIIDRPSQVDPMDEKGNRPENTAGHIRFEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCGKSTLIQLMQRFYDPEAGSVKLDGRDLRTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQADIEKAARAANCHDFITRLPKGYDTQVGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALDPTSEKRVQSALELASQGPTTLVVAHR---------------------LSTITNADKIVFLKDGVVAEQGTHEELMERRGLYCELVSITQRKEATEADEGAV 652 HSP 2 Score: 270.011 bits (689), Expect = 2.723e-75 Identity = 215/661 (32.53%), Postives = 334/661 (50.53%), Query Frame = 0
Query: 71 VSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALI-GLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKA-YGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNL----IFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRT---PAIDSSDMNGDLPAPG---AGNVTFK-------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
VS +L + SP F I + +A+++ G + PL + FGD D DD ++ ++ +I+ G L+ GL N + +Y+F T A R+R +++ QDI ++D N G SR+ D + VQ G ++G + V ++ FV W+ TL+ +P++ +S + G +A++ KA +A VA E ++++RTV + + + ++ + I R+ RG++ +G +L +Y ++ +YG L+ ++ R + +I V +++ G+ +GQA Y + A +A ++ + RT P S N + G NV F+ +LQGL L+I K TVALVGPSG GKST +QL+ R+YDP +G V L+G+ ++ L LR ++G+V QEPVLF TIA+NI YG R+ ++ ++I A K++N ++FI LP+ Y+T +G + +Q+SGGQKQRIAIARALVRNP IL+LDEATSALD +SE VVQ ALD+AR GRT + +AHR L+T+R AD I +K G V E GTH ELM +Y +L + Q
Sbjct: 719 VSFTQLMKLNSPEWRF-IVVGGIASVMHGATFPLWGLFFGDFFGILSDGD--------------DDVVRAEVL----------------------KISMIFVGIGLMAGLGNMLQTYMFTT----AGVKMTTRLRKRAFGTIIGQDIAYFDDERNSVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLVCLSV-YLEGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACRRKVRFRGLVFALGQAAPFL----AYGISMYYGGILVAEE-------------RMNYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILSAGRLMDLFKRTSTQPNPPQSPYNTVEKSEGDIVYENVGFEYPTRKGTPILQGLNLTIKKSTTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTIAENIAYGNNFRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALVRNPKILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHR---------------------LTTVRNADLICVLKRGVVVEHGTHDELMALNKIYANLYLMQ 1298
BLAST of P-glycoprotein vs. nr
Match: gi|586962492|gb|AHK05634.1| (ATP-binding cassette transporter sub-family B member 1 [Tigriopus japonicus]) HSP 1 Score: 1105.12 bits (2857), Expect = 0.000e+0 Identity = 548/744 (73.66%), Postives = 628/744 (84.41%), Query Frame = 0
Query: 67 SLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ-TKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQSERRPSVASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQKNDHKKDKEKY 797
SLPPV++ +LFRFAS RDLFLI LA++AA+I GCSMP+MIILFGDLANTFVQNDLN+TQIC G+P CCDD ++L LP C VTEED+G+F +N NF EQIT FA+GTALIGLVNF+MSY+FVTCLNHAAECQV++IRGLFLK++LRQDIGWYDT+QTGDFASRMTEDLNKVQEG+GEKIGMFIFFATIF+ASLINAFVHGWELTLVI SVMPVLVI+TAIIAGSQTYLTARELKAYGKAGSVAEEVLS+VRTVVAFGGQ+KEV RFE+NL A+KAGIMRG+LTG+GGGFMWL+IY SYALAFWYGVKLIMDDTE C+E++ CDPRYDASSL+VVFFSVLMGAMNVGQATPY+EAFS+ARGAAAQI+ +IDR P IDSS G+ P GAGN+TF+DV L+G+TL INKGETVALVG SGCGKSTVIQLVQRFYDP +G + LNG D+ +L L ALRERIGIVGQEPVLFGCTIA+NIRYGR+GI D DIE ACK+ANAYSFIQ LPKKY+TLVGERGAQ+SGGQKQRIAIARALVRNPDILLLDEATSALD+QSE VVQ+ALDKAR GRTTI+VAHR LSTIRTADKIVA +DGRVAE+GTH ELM +GVYY LV AQ + + D E+E D++ +L+ V+Q+I + G R R++SER+ SVASS+ SDDS T+ED+GHAVGSAVG SRV S Q + +K+ K +
Sbjct: 43 SLPPVAIHRLFRFASSRDLFLIFLAIVAAIIGGCSMPVMIILFGDLANTFVQNDLNVTQICVGIPLCCDDTPAINLDLPNCNVTEEDLGNFFINMNFLEQITTFAQGTALIGLVNFIMSYIFVTCLNHAAECQVFKIRGLFLKAILRQDIGWYDTHQTGDFASRMTEDLNKVQEGIGEKIGMFIFFATIFIASLINAFVHGWELTLVILSVMPVLVIATAIIAGSQTYLTARELKAYGKAGSVAEEVLSAVRTVVAFGGQTKEVERFENNLHDAKKAGIMRGLLTGIGGGFMWLIIYASYALAFWYGVKLIMDDTEDCFEDILHCDPRYDASSLLVVFFSVLMGAMNVGQATPYVEAFSVARGAAAQIFDIIDRVPEIDSSSTAGEHPEKGAGNLTFRDVFFNYPSRKDVKILKGMTLDINKGETVALVGASGCGKSTVIQLVQRFYDPLSGSIMLNGKDLRQLNLSALRERIGIVGQEPVLFGCTIAENIRYGRDGINDSDIEQACKDANAYSFIQSLPKKYDTLVGERGAQLSGGQKQRIAIARALVRNPDILLLDEATSALDTQSEGVVQAALDKARRGRTTIMVAHR---------------------LSTIRTADKIVAFEDGRVAEIGTHGELMKMEGVYYGLVSAQGIQAVDDEDMEEEEDDVTELDMVEQDIFDKGKGNNRVRTESERKMSVASSILSDDSVTMEDIGHAVGSAVGFSRVPS-LQASFYKRQKGSF 764 HSP 2 Score: 350.903 bits (899), Expect = 4.298e-101 Identity = 247/732 (33.74%), Postives = 383/732 (52.32%), Query Frame = 0
Query: 4 LEDLAEEPESFNSNSSCPSLDIGSHKSPRLASDTVPWTENGSNSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIG-MFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVIS----TAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIM----RGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQK-GVYYSLVMAQT 708
+ED+ S S PSL +K + + P + PD+D LP VS+ ++ + A+ ++ + + LLA++I G SMP+ ILFG++ ++ ++ V + C FL + G+V + ++ ++ A E R+R L +++LRQ++ W+D +N TG +R++ D + +Q G +G +F F T+ + S+ A + W+L LV +P ++++ T +I GS + + +A+ + +A E +S++RTV G + FE + A + M R + G+ GF V + +Y+ +YG L+ ++ + Y+N+ + A +LI +G M VGQAT + ++ A AAA+++ ++DR P ID++D G GN+TF VL+ L L++ G+T+ALVGPSGCGKST IQL+QRFYD H G + + G +I L + LR R+GIV QEPVLF T+A+NI YG R D+ ++ A ++AN +SFI LP KY+TLVGE+G Q+SGGQKQR+AIARAL+RNP +LLLDEATSALD++SE VVQ ALDKA+ GRT+I +AHR LSTI+ ++I + GRV E GTH+EL+ +K G+Y L +QT
Sbjct: 733 MEDIGHAVGSAVGFSRVPSLQASFYKRQKGSFTDTPL--------ESPDED-------------LPKVSMIRILK-ANSKEWPYMLIGLLASVIMGASMPVYAILFGEVLGVLSEDPVSARD---NVSYYCILFL-------------------------------------ITGMVVGIAMFLQISMFTLAGEHLTLRMRKLAFEAMLRQEMAWFDLPSNSTGALCTRISSDASAIQGASGSPLGTLFQSFFTLTI-SIGLAMYYQWQLGLVTSVFIPFVLVALYFQTKMIMGSDSV----QKEAFASSAKLAIEAISNIRTVAGLGREKT----FEELYLNALRQPHMDAKKRSHVRGLIFGFAQSVPFFAYSGCMFYGGWLV-ENQDLDYKNVFKV-----AEALI-------LGTMMVGQATAFAPNYNKALLAAARVFKLLDRKPKIDANDATGLRINDIQGNITFSQAGFHYPTRKEVRVLRELNLAVQAGQTIALVGPSGCGKSTCIQLLQRFYDLHKGVLTVEGQNIQSLNVPQLRSRMGIVSQEPVLFDRTLAENIAYGDNSRTASMDEVVD-AARQANIHSFISSLPLKYDTLVGEKGTQLSGGQKQRVAIARALIRNPAVLLLDEATSALDTESEKVVQEALDKAQKGRTSITIAHR---------------------LSTIQNVNRIFVISKGRVVEAGTHNELLARKEGLYAKLWGSQT 1358
BLAST of P-glycoprotein vs. nr
Match: gi|406654432|gb|AFS49708.1| (P-glycoprotein [Tigriopus japonicus]) HSP 1 Score: 1063.14 bits (2748), Expect = 0.000e+0 Identity = 532/744 (71.51%), Postives = 609/744 (81.85%), Query Frame = 0
Query: 67 SLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ-TKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQSERRPSVASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQKNDHKKDKEKY 797
SLPPV++ +LFRFAS RDLFLI LA++AA+I GCSMP+MIILFGDLANTFVQNDLN+TQIC G+P CCDD ++L LP C VTEED+G+F +N NF EQIT FA+GTALIGLVNF+MSY+FVTCLNHAAECQV++IRGLFLK++LRQDIGWYDT+QTGDFASRMTEDLNKVQEG+GEKIGMFIFFATIF+ASLINAFVHGWELTLVI SVMPVLVI+TAIIAGSQTYLTARELKAYGKAGSVAEEVLS+VRTV KAGIMRG+LTG+GGGFMWL+IY SYALAFWYGVKLIMDDTE C+E++ CDPRYDAS L+VVFFSVLMGAMNVGQATPY+EAFS+ARGAAAQI+ +IDR P IDSS G+ P GAGN+TF+DV L+G+TL INKGETVALVG SGCGKSTVIQLVQRFYDP +G + LNG D+ +L L ALRERIGIVGQEPVLFGCTIA+NIRYGR+GI D DIE ACK+ANAYSFIQ LPKKY+TLVGERGAQ+SGGQKQRIAIARALVRNPDILLLDEATSALD+QSE VVQ+ALDKAR GRTTI+VAHR LSTIRTADKIVA +DGRVAE+GTH ELM +GVYY LV AQ + + D E+E D++ +L+ V+Q+I + G R R++SER+ SVASS+ SDDS T+ED+GHAVGSAVG SRV S Q + +K+ K +
Sbjct: 43 SLPPVAIHRLFRFASSRDLFLIFLAIVAAIIGGCSMPVMIILFGDLANTFVQNDLNVTQICVGIPLCCDDTPAINLDLPNCNVTEEDLGNFFINMNFLEQITTFAQGTALIGLVNFIMSYIFVTCLNHAAECQVFKIRGLFLKAILRQDIGWYDTHQTGDFASRMTEDLNKVQEGIGEKIGMFIFFATIFIASLINAFVHGWELTLVILSVMPVLVIATAIIAGSQTYLTARELKAYGKAGSVAEEVLSAVRTV---------------------KAGIMRGLLTGIGGGFMWLIIYASYALAFWYGVKLIMDDTEDCFEDILHCDPRYDASGLLVVFFSVLMGAMNVGQATPYVEAFSVARGAAAQIFDIIDRVPEIDSSSTAGEHPEKGAGNLTFRDVFFNYPSRKDVKILKGMTLDINKGETVALVGASGCGKSTVIQLVQRFYDPLSGSIMLNGKDLRQLNLSALRERIGIVGQEPVLFGCTIAENIRYGRDGINDSDIEQACKDANAYSFIQSLPKKYDTLVGERGAQLSGGQKQRIAIARALVRNPDILLLDEATSALDTQSEGVVQAALDKARRGRTTIMVAHR---------------------LSTIRTADKIVAFEDGRVAEIGTHGELMKMEGVYYGLVSAQGIQAVDDEDMEEEEDDVTELDMVEQDIFDKGKGNNRVRTESERKMSVASSILSDDSVTMEDIGHAVGSAVGFSRVPS-LQASFYKRQKGSF 743 HSP 2 Score: 350.903 bits (899), Expect = 3.977e-101 Identity = 247/732 (33.74%), Postives = 383/732 (52.32%), Query Frame = 0
Query: 4 LEDLAEEPESFNSNSSCPSLDIGSHKSPRLASDTVPWTENGSNSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIG-MFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVIS----TAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIM----RGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQK-GVYYSLVMAQT 708
+ED+ S S PSL +K + + P + PD+D LP VS+ ++ + A+ ++ + + LLA++I G SMP+ ILFG++ ++ ++ V + C FL + G+V + ++ ++ A E R+R L +++LRQ++ W+D +N TG +R++ D + +Q G +G +F F T+ + S+ A + W+L LV +P ++++ T +I GS + + +A+ + +A E +S++RTV G + FE + A + M R + G+ GF V + +Y+ +YG L+ ++ + Y+N+ + A +LI +G M VGQAT + ++ A AAA+++ ++DR P ID++D G GN+TF VL+ L L++ G+T+ALVGPSGCGKST IQL+QRFYD H G + + G +I L + LR R+GIV QEPVLF T+A+NI YG R D+ ++ A ++AN +SFI LP KY+TLVGE+G Q+SGGQKQR+AIARAL+RNP +LLLDEATSALD++SE VVQ ALDKA+ GRT+I +AHR LSTI+ ++I + GRV E GTH+EL+ +K G+Y L +QT
Sbjct: 712 MEDIGHAVGSAVGFSRVPSLQASFYKRQKGSFTDTPL--------ESPDED-------------LPKVSMIRILK-ANSKEWPYMLIGLLASVIMGASMPVYAILFGEVLGVLSEDPVSARD---NVSYYCILFL-------------------------------------ITGMVVGIAMFLQISMFTLAGEHLTLRMRKLAFEAMLRQEMAWFDLPSNSTGALCTRISSDASAIQGASGSPLGTLFQSFFTLTI-SIGLAMYYQWQLGLVTSVFIPFVLVALYFQTKMIMGSDSV----QKEAFASSAKLAIEAISNIRTVAGLGREKT----FEELYLNALRQPHMDAKKRSHVRGLIFGFAQSVPFFAYSGCMFYGGWLV-ENQDLDYKNVFKV-----AEALI-------LGTMMVGQATAFAPNYNKALLAAARVFKLLDRKPKIDANDATGLRINDIQGNITFSQAGFHYPTRKEVRVLRELNLAVQAGQTIALVGPSGCGKSTCIQLLQRFYDLHKGVLTVEGQNIQSLNVPQLRSRMGIVSQEPVLFDRTLAENIAYGDNSRTASMDEVVD-AARQANIHSFISSLPLKYDTLVGEKGTQLSGGQKQRVAIARALIRNPAVLLLDEATSALDTESEKVVQEALDKAQKGRTSITIAHR---------------------LSTIQNVNRIFVISKGRVVEAGTHNELLARKEGLYAKLWGSQT 1337
BLAST of P-glycoprotein vs. nr
Match: gi|1325288603|ref|XP_023329072.1| (multidrug resistance protein 1-like isoform X1 [Eurytemora affinis]) HSP 1 Score: 818.535 bits (2113), Expect = 0.000e+0 Identity = 413/730 (56.58%), Postives = 532/730 (72.88%), Query Frame = 0
Query: 90 LALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQIC---QGVPFCCDDFLQLDLSLPGCEVTEE--DIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGE--DEIDEIQDLETV-DQEISEMGT----AGP-RNRSQSERRPSVAS--SVFSDDSFTLEDVGHAVGSAVGLSRVVSDRQKNDHKK 792
+ +LAA+ GCSMP+MIILFG LAN FV T+ C +GV +SLP C E D+G + F+ +I++F G ++IGLVN + SY+FVTCLNHAAE QV+RIR +FLK++LRQDIGWYD +QTGDFA+++T+DLNK+QEG+GEKIGMFIFF TIF+ASL NAF+HGWELTLVI MPVL+I+ +A +Q LT +E AYG+AGS+A+EVL ++RTV+AFGG+ +EV R++ L+FARKAGIMRGMLTGVG G MW +IY SYALAFWYGVKLIMDD E C + A C+PRY SSL++VFFSVLMGAMN+GQA+PY+EAFS+A+G+AA+IYS++DR P IDSS G P AG + DV L+GL+L+I G+TVALVGPSGCGKSTVIQ++QRFYDP GGV L+G+D+ + L LR++IG+VGQEPVLFG +IA+NIR+G+EG+T+++I ACKEANAY FIQ LPKK TLVG++GAQ+SGGQKQRIAIARALVRNP ILLLDEATSALD+ SEA VQ+ALD+A+ GRTTI+VAHR LST+R+ADKI+A+KDG+V E GTH +LM ++G+Y+SLV Q KEE D E D+ + ++++E++ + E+ + + GP R +S +R+ SV S S +S+DS ED A SAVG++R S KK
Sbjct: 2 IGVLAAVAGGCSMPVMIILFGQLANAFVSE--AQTEGCTNAEGV---------FSISLPNCTFDPEALDLG----KDLFYAEISKFGGGASIIGLVNLISSYLFVTCLNHAAESQVFRIRTMFLKAILRQDIGWYDVHQTGDFANKITDDLNKLQEGIGEKIGMFIFFLTIFIASLTNAFIHGWELTLVILGAMPVLIIAIGCLARAQAALTEKETAAYGRAGSIAQEVLGTIRTVIAFGGEEREVARYKSKLVFARKAGIMRGMLTGVGAGLMWFIIYSSYALAFWYGVKLIMDDRELCMLDPAGCNPRYTPSSLLIVFFSVLMGAMNLGQASPYVEAFSIAKGSAAKIYSIMDRIPPIDSSSTAGVRPEQLAGKIQINDVHFSYPSRSEVPILKGLSLTIEPGKTVALVGPSGCGKSTVIQMIQRFYDPSTGGVVLDGVDLKNVNLGWLRDQIGVVGQEPVLFGTSIAENIRFGKEGVTEEEITQACKEANAYGFIQKLPKKMETLVGDKGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTASEACVQAALDRAKCGRTTIIVAHR---------------------LSTVRSADKIIAIKDGQVEEEGTHDQLMLKQGLYFSLVTTQMSKEEDPDPEELDDYEPLREMESLTESELPSLNSISSFTGPFRKKSAVDRKISVLSNYSTYSEDSLGFEDALEAATSAVGIARAGSTSTSRRSKK 695 HSP 2 Score: 338.576 bits (867), Expect = 6.638e-97 Identity = 241/720 (33.47%), Postives = 366/720 (50.83%), Query Frame = 0
Query: 16 SNSSCPSLD-IGSHKSPRLASDTVPWTENGSNSGKDPDK-------DEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDT--NQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTA----IIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG--REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
SN S S D +G + A+ V GS S K E+ E V+E+ L V ++ + S F++ + ++ +++ G +MP+ ILFG++ T L LS TEE EQ F+ G+ + ++ ++ A E R+R L ++LRQ++GW+D N G SR+ D + +Q G +IG + A + S+ + V W L L+ +P+++I T II+ + E A KA +A E + ++RTVV +S + +E L+ +A + L G+ GF + + +YA +YG L+ + ENL D + V ++++G M VGQA+ + ++ A AAA+++++IDR P IDS+ +G G + F++ VL+ L L+I+ G++VALVGPSGCGKST IQL+QRFYD + G + L DI+ L + +R ++ IV QEPVLF T+A+NI YG + +T +++ A ++AN + FI LP+ Y T VGE+G Q+SGGQKQRIAIARALVRNP I+LLDEATSALD++SE VQ+ALD A+ GRT+I +AHR LST++ D I V GRV E G+H +L+ +KG Y+ L Q
Sbjct: 656 SNYSTYSEDSLGFEDALEAATSAVGIARAGSTSTSRRSKKSVVRMESESLALPESVEEE-LVQVDSLRIMKMNSKEWPFVV-VGMIGSIVMGGAMPVYAILFGEVLGT------------------------LKLS------TEEA----------REQSVYFSGLFLATGITAGIAIFLQISMFTVAGEYLTQRMRKLAFAAMLRQEMGWFDRPENSVGALLSRLAADASAIQGATGSRIGAILHAAFTLIISITTSLVLEWRLGLLGCVFVPLVLIGTMAQFRIISAQDSV----EKSALQKASKLAIEAIGNIRTVVGLRKESFFINEYEKELLGPHEASKTKSHLRGIIFGFSQSMPFFAYAGCMYYGGYLVYN------ENL-------DYKLVFKVAEALILGTMMVGQASAFAPNYTKAMIAAARVFNLIDRKPLIDSAAGSGLRLNKVEGEIEFRNAVFHYPNRSQVRVLKNLNLAISTGKSVALVGPSGCGKSTCIQLLQRFYDLNDGDITLENHDINALNVPWVRSKLSIVSQEPVLFNRTLAENISYGDNTKDVTMEEVIAAARKANIHQFITNLPQGYETNVGEKGTQLSGGQKQRIAIARALVRNPPIILLDEATSALDTESEKTVQTALDSAQKGRTSITIAHR---------------------LSTVKNCDTIFVVNHGRVVEYGSHDDLLQKKGTYHKLWSTQ 1295
BLAST of P-glycoprotein vs. nr
Match: gi|314912068|gb|ADT63773.1| (P-glycoprotein [Lepeophtheirus salmonis]) HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0 Identity = 400/756 (52.91%), Postives = 517/756 (68.39%), Query Frame = 0
Query: 71 VSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQIC---QGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAI----DSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREG-ITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELM-NQKGVYYSLVMAQTKKEETRDG---------EDEIDEIQDLETVDQEISEM-------GTAGPRN----RSQSERRPSVASSVFSDDSFTLEDVGHAVGSAVGLSRVVSDR 785
V KLF +A+ DL I + +LAALI G S+P MI+LFG+L +TF+ ++ T IC G CC + +DLSL C++ E+DI NF + + RF +GTA+IGL+NF+ SY+FVT LN AE QV+RIR F KS+L QDI W+DT++TGDFA+++TEDLNK+QEG+GEKIG+FIFF TIF++SLI AF+HGWELTLVI S MP+L+I+ IIA SQT LT +E AY KAGSVAEE SS++TV++F GQ+ E+ R++ NL A+K GI+RG+LTG+GGG MW +IY SYA+AFWYGVKLI+DD E C + +C RY S+L++VFFSVLMGAMN+GQA+PY+EAF++ARGAA+ ++ +I TPAI D D P G +TFK+ VL GL+ + G+T+ALVGPSGCGKSTVIQL+QRFYDP G V ++G DI+ L LR IGIVGQEPVLF +I +NI G +G I++K I+ CK ANAY FIQ LPKKY+T+VGE+GA +SGGQKQRIAIARAL+RNP ILLLDEA+SALDSQSE +VQSALDKAR GRTTI+VAHR LSTIR+AD I+ +KDG + GTH L N+ G+Y SLV AQ + + +G +E DE+ DLE V+ E++ M G++ R R ERR S S +S+DS +ED GSA+G++RV S +
Sbjct: 80 VPFMKLFSYATRSDLIFIGIGILAALIGGLSLPFMIMLFGELTDTFILSNPLSTDICLIENGT--CCSNNGTVDLSLEDCDLNEDDIMQLFKPINFLDGVARFGQGTAIIGLINFITSYIFVTSLNFTAERQVHRIRKAFFKSLLNQDIKWFDTHETGDFATKITEDLNKLQEGIGEKIGLFIFFITIFISSLITAFIHGWELTLVILSAMPILMIAVGIIAKSQTALTVKESNAYSKAGSVAEEAFSSIKTVMSFQGQNTEIQRYKENLSEAQKTGILRGLLTGIGGGLMWFIIYSSYAIAFWYGVKLILDDRESCIASPTDCQIRYGPSNLLIVFFSVLMGAMNIGQASPYVEAFAIARGAASSVFQIIQSTPAIKSDYDHLQRQQDKAPPFTGRITFKNVHFEYPSRPTVKVLSGLSFEASPGKTLALVGPSGCGKSTVIQLIQRFYDPSFGVVSIDGEDITTLDPHWLRSHIGIVGQEPVLFEYSIKENITMGLQGEISEKMIDDTCKAANAYDFIQRLPKKYDTIVGEKGALLSGGQKQRIAIARALIRNPSILLLDEASSALDSQSEFIVQSALDKARKGRTTIIVAHR---------------------LSTIRSADAILVMKDGYRVDYGTHESLKSNKTGLYCSLVNAQDCQVDQDEGLPLFNPELNYEEEDEVYDLEQVENEMNSMTYGSISGGSSWNRRHHFVRPTLERRHSTGSG-YSEDSLKIEDALDVAGSAIGIARVGSRK 811 HSP 2 Score: 290.812 bits (743), Expect = 1.232e-79 Identity = 197/571 (34.50%), Postives = 296/571 (51.84%), Query Frame = 0
Query: 176 LIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQE----------------GMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDS----------SDMNGDLPAPGAGNV--TFKDV--LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKE 711
L+G++ ++ + + + E ++R ++L Q++GWYD N TG SR++ D + VQ G ++G I + S+ A +L LV +P ++I T E A ++ +A E ++ +RTVV + ++ L ++ I L G+ GF + + +YA +YG L+ D Y+N+ + A +LI +G + VGQAT + ++ AR A+ +I+ +++R P I S ++MNG++ AG T K V L+ L LSI G+++ +VG SGCGKST+IQL+Q+FYD +G + L+ D + + LR +IGIV QEP LF +I +NI YG R ++ DI A K+AN +SFI LP+ Y+T VG G +SGGQKQRIAIARAL+RNP +LLLDEATSALD++SE VVQ AL+KA RT+I +AHR LSTI+ DKI + G+VAE G+H L+ KG YY L T+++
Sbjct: 901 LVGIIVGFSMFMQIFMFSLSGELLTTKLRIKAFTAMLNQEVGWYDESVNSTGALCSRLSADASAVQGVINYLSFFIHLLYILIATGSRLGTIIQVTLTILMSISAALYFNIKLGLVGTLFVPFVLIGAWFQGKIITSQDNLEKDALSRSARIAIEAINGIRTVVGLRLEESFQEKYSTELKDPHESAIKNSHLRGLIFGFSQSIPFFAYAGTMYYGGTLVESDG-LPYKNVFKV-----AETLI-------LGTLMVGQATAFGPNYTKARIASIRIFKLLNREPKIRSDVIPNTDEMATNMNGEVTFTNAGFYYPTRKSVKVLRDLKLSIKSGQSIGIVGSSGCGKSTIIQLIQKFYDLSSGKLELDSKDSESINVMWLRSKIGIVSQEPNLFNRSIRENICYGLNKRNDVSMDDIIQAAKDANIHSFIASLPQGYDTRVGNAGTMLSGGQKQRIAIARALIRNPSLLLLDEATSALDTESEKVVQEALNKALENRTSITIAHR---------------------LSTIKNVDKIFVLNQGKVAEAGSHESLLLLKGFYYKLWTNGTQRK 1437
BLAST of P-glycoprotein vs. nr
Match: gi|636629987|gb|AIA09022.1| (ABC transporter, partial [Eulimnogammarus verrucosus]) HSP 1 Score: 703.36 bits (1814), Expect = 0.000e+0 Identity = 368/695 (52.95%), Postives = 476/695 (68.49%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDL--NMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTK--------KEETRDGEDEIDEIQDLETVDQEISEMGTAGP 741
PPV + ++FR+A+ ++ LI L LLAA + G MP MIILFGD+ N FV +DL N+T I QL+ S T +D H + +FF Q+ RF GT +IG++ + YVFVTCLN+AAE QV+RIR FL++ +RQ++GW+DT++T DFAS++TEDLNK+QEG+GEKIGMF+FF F+ASLINAFVHGWELTLVI SV P+L I++ I+A Q+ LT+RE+ Y KAGS+AEEV+ +RTV+AFGGQ KEV R+E NLI AR AGI RG+ TG+ G +WL+IY SY++AFWYG LI+ E Y N D YD ++LIVVFFSVLMGAMN+GQA P++EAF++ARGAAA +Y V++R ID G P G + F++ VLQGL L + G+TVALVG SGCGKST +QL+QRFYDP G V L+ + + + + LR+++G+VGQEPVLF TI +NIRYGR+G++ + A +EANA+ FI +PK+Y T VGERG+QMS GQKQRIAIARALV+ P ILLLDEATSALD+QSEAVVQ+ALDKAR GR+TI+VAHR L+TIR+AD IVA++DGR AEMGTH +LM + G+YYSLV AQ EE G E + +LE D + E+ +A P
Sbjct: 66 PPVPMTQMFRYANLKERSLIFLGLLAATVAGLCMPAMIILFGDITNAFVYSDLDINITAIAA----------QLNKSEDYVNATLQD--H--MPGDFFTQVVRFGAGTGIIGVIQLLTGYVFVTCLNYAAEGQVHRIRTQFLRATMRQEMGWFDTHKTNDFASKVTEDLNKLQEGIGEKIGMFVFFFVTFIASLINAFVHGWELTLVIMSVFPLLGITSGIMAKIQSSLTSREMAEYAKAGSIAEEVMGGIRTVLAFGGQEKEVKRYESNLIHARAAGIKRGLFTGISMGLVWLIIYASYSIAFWYGTGLIL---ESRYPN----DGEYDPATLIVVFFSVLMGAMNMGQAAPFMEAFNIARGAAANVYEVLERPSLIDPYSEEGIRPKDTNGFIEFRNVHFSYPARPDVKVLQGLNLQVRPGQTVALVGSSGCGKSTCVQLMQRFYDPANGSVLLDSVPLHEHNIGWLRDQVGVVGQEPVLFATTIENNIRYGRDGLSFDQVVAAAREANAHDFIMKMPKQYQTQVGERGSQMSSGQKQRIAIARALVKQPRILLLDEATSALDNQSEAVVQNALDKARQGRSTIIVAHR---------------------LTTIRSADLIVAMQDGRAAEMGTHEQLMVKHGLYYSLVTAQIAPGDRKSVIHEELSAG-SEYETAPELEQHDI-LEELTSAAP 716
BLAST of P-glycoprotein vs. nr
Match: gi|1067077737|ref|XP_018022498.1| (PREDICTED: LOW QUALITY PROTEIN: multidrug resistance protein 1-like [Hyalella azteca]) HSP 1 Score: 700.664 bits (1807), Expect = 0.000e+0 Identity = 373/716 (52.09%), Postives = 492/716 (68.72%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRI--RGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFK------------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDG-EDEIDEIQDLETVDQ-------EISEMGTA---GPRNRSQSERRPSVASSVFS 759
PPV VF+LFR+++ + LI LAL AA + G MP MIILFGD+ N FV +DLN+T I DL++ EV + ++FF ++ RF GTA+IG V V Y+FVTCLN+AAE QV + R FL+ LRQ++GW+DT++T DFAS++TEDLNK+QEGMGEK+GMF+FF F+A++INAFVHGWELTLVI SV P+L I+T +I+ Q+ LT +E+ Y KAGS+AEEVLS ++TV+AFGG++KEV R+E NL+ AR AG+ RGM +G+ G +WL+IY SY++AFWYG LI+D Y N D YD + LIVVFFSVL+GA+N+GQ P++EAF++ARGAAA +Y +++R AID +G P GN+ F+ VLQGL+L I GETVALVG SGCGKST IQL+QRFYDP G V L+ +D+ L + LR++IG+VGQEPVLF TI +NIRYGR+GI+ +D+ A K+ANA+ F+ LP++Y T VG+RGAQMSGGQKQR+AIARALV+ P +LLLDEATSALD+QSE++VQ+ALDKAR GRTTI+VAHR L+TIR+AD IVA+KDGR EMGTH ELM ++G+Y+SLV AQ E + E+++ E + ET + +IS++ + P RS S++R S A SV S
Sbjct: 109 PPVGVFQLFRYSTCTERALIILALAAASLAGLCMPAMIILFGDITNAFVYSDLNLTVIAH------------DLNMTVEEVNATFQAMY--TDDFFSEVIRFGAGTAIIGGVQLVTGYIFVTCLNYAAEGQVXPVYNRTQFLRCTLRQEMGWFDTHKTNDFASKVTEDLNKLQEGMGEKVGMFVFFFVTFIANVINAFVHGWELTLVIMSVFPLLAITTGLISKIQSSLTTQEMAEYAKAGSIAEEVLSGIKTVLAFGGETKEVKRYESNLVHARNAGVRRGMFSGISLGLVWLIIYASYSIAFWYGTGLILDSR---YPN----DGDYDPAVLIVVFFSVLVGALNMGQVAPFVEAFNIARGAAANVYDILERPSAIDPMSEDGLRPNEVHGNIEFRRVHFEYPSRSDVPVLQGLSLRIQPGETVALVGSSGCGKSTCIQLLQRFYDPRQGEVTLDSVDVRSLNIGWLRDQIGVVGQEPVLFATTIEENIRYGRDGISFEDVVAAAKQANAHDFVMKLPQQYQTQVGDRGAQMSGGQKQRLAIARALVKRPKVLLLDEATSALDNQSESIVQNALDKARQGRTTIIVAHR---------------------LTTIRSADVIVALKDGRAEEMGTHDELMAKRGLYHSLVTAQLAPSERKSALEEDLTEQSEYETATELDRDVLDDISDITASLAMSPARRSISKKRRSSAVSVRS 782 HSP 2 Score: 336.65 bits (862), Expect = 7.707e-96 Identity = 231/660 (35.00%), Postives = 333/660 (50.45%), Query Frame = 0
Query: 61 EKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDT--NQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSL 703
EK KEK PP + A+ + I L ++ ++I G PL +LFGD+ T L ++ P E NF+ + IG+V + +++ + E R+R +L Q++GW+D N G SR++ D VQ G ++G + T SL A + W L LV +PV++++ + + + + E + AG +A E +S+VRTV + + R+ L + + L G GF V + +YA+ +YG L+ D YEN+ + A +LI +G M VGQA + ++ + AAA+I+ ++ R P IDSS G V DV LQGL+L ++KG+T+ALVG SGCGKSTVI L++RFYDP G + +N ++ L + ++R +G+V QEP+LF +I +NI YG RE D +I A K+AN SFI+ LP+KYNT VG +G Q+SGGQKQRIAIARALVRNP +L+LDEATSALD++SEAVVQ ALDKA+ GRT+IV+AHR LSTI+ AD I + G++ E GTH +LM YY L
Sbjct: 802 EKKKEK--PPTLPMREILKANKTEWPQITLGVVGSVIMGSCTPLYAVLFGDVLGT------------------------LSIADPAEARAEA---------NFYALLF------LCIGIVAAISTFMQAYFFALSGEILTMRLRSRAFSQMLSQELGWFDMDENSVGALCSRLSSDAAAVQGATGSRLGTIMQALTTLGLSLGLALYYDWRLGLVCVPFIPVVLVAVYLQSKILSGQSITESEVLQSAGKIAMEAISNVRTVASLHKEEHFSQRYAEALHEPHRLALRNSHLRGAAFGFAQCVPFVAYAVVMFYGGYLV-DKGTMPYENVFKV-----AEALI-------LGTMMVGQAVAFAPNYAKGKVAAARIFKLLARVPLIDSSPSVGRTLKEEFDEVRLHDVHFRYPNREDVQILQGLSLVVSKGKTLALVGSSGCGKSTVISLIERFYDPEKGKLTINNEELKTLNVGSVRRGVGLVSQEPLLFDLSIRENIAYGDNSREVPFD-EIVAAAKQANISSFIESLPEKYNTRVGSKGTQLSGGQKQRIAIARALVRNPSVLMLDEATSALDTESEAVVQEALDKAQAGRTSIVIAHR---------------------LSTIQGADTIAVISSGKLVESGTHDQLMALGQEYYRL 1385
BLAST of P-glycoprotein vs. nr
Match: gi|636629993|gb|AIA09025.1| (ABC transporter, partial [Gmelinoides fasciatus]) HSP 1 Score: 700.664 bits (1807), Expect = 0.000e+0 Identity = 358/668 (53.59%), Postives = 462/668 (69.16%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDL--NMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDE 722
PPV + ++FR+A+ ++ LI L +LAA + G MP MIILFGD+ N FV +DL N+T I QL+ S T +D + +FF Q+ RF GTA+IG + Y FVTCLN+AAE QV+RIR FL++ LRQ++GW+DT++T DFAS++TEDLNK+QEG+GEKIGMF+FF F+AS+INAFVHGWELTLVI SV P+L I++ ++A Q+ LT+RE+ Y KAGS+AEEV+ +RTV+AFGGQ KEV R+E NLI AR AGI RG+ TG+ G +WL+IY SY++AFWYG LI+ E+ D YD + LIVVFFSVLMGAMN+GQA P++EAF++ARGAAA +Y V++R ID M G P G + F+D VLQGL L + G+TVALVG SGCGKST +QL+QRFYDP G V L+ +D+ + + LR+++G+VGQEPVLF T+ DNIRYGR+G++ + + A KEANA+ FI +PK+Y T VGERG+QMSGGQKQRIAIARALV+ P ILLLDEATSALD+QSE+VVQ ALDKAR GR+TI+VAHR L+TIR+AD IVA++DG EMGTH +LM + G+YYSLV AQ DG+ + E
Sbjct: 130 PPVPMTQMFRYANTKERSLIFLGMLAATVAGLCMPAMIILFGDITNAFVYSDLDINLTVIAA----------QLNKSEDYVNATIQDF----MPGDFFTQVIRFGAGTAIIGGIQLFTGYCFVTCLNYAAEGQVHRIRTQFLRATLRQEMGWFDTHKTNDFASKVTEDLNKLQEGIGEKIGMFVFFFVTFLASIINAFVHGWELTLVIMSVFPLLAITSGVMAKIQSSLTSREMAEYAKAGSIAEEVMGGIRTVLAFGGQEKEVKRYESNLIHARAAGIKRGLFTGISMGLVWLIIYASYSIAFWYGTGLIL-------ESRLPNDGEYDPAVLIVVFFSVLMGAMNMGQAAPFMEAFNIARGAAANVYEVLERQSLIDPYSMEGIRPKDTNGFIEFRDVHFSYPSRPDVKVLQGLNLQVRPGQTVALVGSSGCGKSTCVQLMQRFYDPANGSVLLDSVDLREHNIGWLRDQVGVVGQEPVLFATTVEDNIRYGRDGLSFEQVVAAAKEANAHDFIMKMPKQYQTQVGERGSQMSGGQKQRIAIARALVKQPRILLLDEATSALDNQSESVVQLALDKARQGRSTIIVAHR---------------------LTTIRSADLIVAMQDGLAVEMGTHEQLMVKHGLYYSLVTAQVPPG---DGKSVVHE 752
BLAST of P-glycoprotein vs. nr
Match: gi|636629985|gb|AIA09021.1| (ABC transporter, partial [Gammarus lacustris]) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 366/695 (52.66%), Postives = 473/695 (68.06%), Query Frame = 0
Query: 69 PPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDL--NMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKD------------VLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTK--------KEETRDGEDEIDEIQDLETVDQEISEMGTAGP 741
PPVS+ ++FR+A+ ++ LI + +LAA + G MP MIILFGD+ N FV +DL N+T+I QL+ S T +D + +FF Q+ RF GTA+IG + + YVFVTCLN+A E QV+RIR FL++ +RQ++GW+DT +T DFAS++TEDLNK+QEG+GEKIGMF+FF F+ASLINAFVHGWELTLVI SV P+L I+T ++A Q+ LT+RE+ Y KAGS+AEEV+ +RTV+AFGGQ KEV R+E NLI AR AGI RG+ TG+ G +WL+IY SY++AFWYG LI+ E Y N D YD + LIVVFFSVLMGAMN+GQA P++EAF++ARGAAA +Y V++R ID G P G + F+D VLQGL L + G+TVALVG SGCGKST +QL+QRFYD +G V L+ +D+ + + LR+++G+VGQEPVLF TI +NIRYGR+G++ + A KEANA+ FI +P +Y T VGERG+QMSGGQKQRIAIARALV+ P ILLLDEATSALD+QSEAVVQ+ALDKAR GR+TI+VAHR L+TIR+AD IVA++DGR EMGTH +LM + G+Y+SLV AQ EE G E + +LE D + E+ A P
Sbjct: 136 PPVSMSQIFRYANLKERSLIFVGMLAATVAGLCMPAMIILFGDITNAFVYSDLEINITEIAA----------QLNKSEDYVNATIQDN----MPGDFFTQVVRFGAGTAIIGGIQLLTGYVFVTCLNYATEGQVHRIRTQFLRATMRQEMGWFDTRKTNDFASKVTEDLNKLQEGIGEKIGMFVFFFVTFIASLINAFVHGWELTLVIMSVFPLLAITTGVMAKIQSSLTSREMAEYAKAGSIAEEVMGGIRTVLAFGGQEKEVMRYESNLIHARTAGIKRGLFTGISMGLVWLIIYASYSIAFWYGSGLII---ESRYPN----DGEYDPAVLIVVFFSVLMGAMNMGQAAPFMEAFNVARGAAANVYEVLERQSLIDPYSEEGIRPKDTNGFIEFRDVHFSYPSRPDVKVLQGLNLQVRPGQTVALVGSSGCGKSTCVQLMQRFYDTASGSVLLDSVDLREHNIGWLRDQVGVVGQEPVLFATTIENNIRYGRDGLSFDQVVAASKEANAHDFIMKMPMQYQTQVGERGSQMSGGQKQRIAIARALVKQPRILLLDEATSALDNQSEAVVQNALDKARQGRSTIIVAHR---------------------LTTIRSADLIVAMQDGRAVEMGTHEQLMVKHGLYHSLVTAQVAPIDRKSVIHEEVSAG-SEYETAPELEQQDI-LEELTNAAP 786
BLAST of P-glycoprotein vs. nr
Match: gi|321474272|gb|EFX85237.1| (ABC transporter, subfamily ABCB/MDR [Daphnia pulex]) HSP 1 Score: 697.967 bits (1800), Expect = 0.000e+0 Identity = 371/713 (52.03%), Postives = 474/713 (66.48%), Query Frame = 0
Query: 74 FKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQ-GVPFCCDDFLQLDLSL-PGCEVTE-EDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPR-YDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFKDV------------LQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDGEDEIDEIQDLETVDQEISEMGTAGPRNRSQSER--RPSVASSVFSDDSFTLEDV 768
F LFR+AS D L+C L A+ G PLM+ILFGD+ N FV ++ I + D L P C +++ + + ++ T+F A+IG V F++ ++FVT LN AE QVYRIR FL++VLRQD+GWYDT + DFASR+TEDLNK+Q+G+GEKIGMFIF T F+AS+INAF+HGWELTLV+ PVL +S ++A Q LT ELKAY KAG +AEEV SS+RTV+AFGGQ KE+ RF+ +L +A+KAGI RGM TG+G G +W +IY SYALAFWYG+ LI LA CD Y +S L++VFFSVL+GAM +GQA PY+EAFS+ARGAAA I+++IDR P IDSS G +P G ++F+DV LQG++ + G+TVALVG SGCGKST IQL+QRFYDP G V ++G ++ L L LR+++G+VGQEPVLFG +I +NI YGR+G++ +++E A KEANA+ FIQ LP+KY+TLVGERG Q+SGGQKQRIAIARALVR P ILLLDEATSALD+QSEAVVQ ALDKAR GRTTI+VAHR L+TIR AD+I+ +KDG V E GTH +LM G+YY LV+AQ E D + E +E+ D + + + P R S R R SVASS S S ED+
Sbjct: 69 FDLFRYASSTDFVLLCFGTLGAVGTGVCFPLMLILFGDITNAFVGGGMDQETINEINCNISSDPNYTYPFPLGPTCNISDPSEFANSPQGQAVQDEFTKFGIYVAIIGAVLFLLGFIFVTALNFTAENQVYRIRSKFLQAVLRQDVGWYDTKSSNDFASRITEDLNKIQDGVGEKIGMFIFSMTCFIASIINAFIHGWELTLVMLVSTPVLAVSMGVLAKVQASLTENELKAYAKAGGIAEEVFSSIRTVMAFGGQRKEIDRFQDDLAYAKKAGIKRGMATGIGAGLVWGIIYASYALAFWYGITLI----------LAACDGNSYSSSDLLIVFFSVLIGAMQIGQAAPYMEAFSVARGAAATIFAIIDRVPPIDSSSNEGLVPDGVDGKISFRDVFFNYPSRPDVKILQGISFDVTPGQTVALVGTSGCGKSTCIQLLQRFYDPLEGSVTIDGNELRNLNLGWLRDQMGMVGQEPVLFGTSIGENICYGRDGVSKEEMERAAKEANAHDFIQRLPRKYDTLVGERGGQLSGGQKQRIAIARALVRQPKILLLDEATSALDTQSEAVVQKALDKARQGRTTIIVAHR---------------------LTTIRNADRIIVMKDGIVQEDGTHDKLMALNGIYYQLVIAQQGGES--DSKKEKEEMMDAVS-------LAGSHPLGRHNSVRSARLSVASSAVSAQS---EDI 738 HSP 2 Score: 371.703 bits (953), Expect = 9.434e-109 Identity = 253/716 (35.34%), Postives = 371/716 (51.82%), Query Frame = 0
Query: 9 EEPESFNSNSSCPSLDIGSHKSPRLASDTVPWTENGSNSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYD--TNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFK------------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQ 707
E+ E ++ S S +G H S R A +V + + S + D D VS+ + R + ++ I + ++ + I G S P+ ILF ++ L + PG E+ NF+ + F ++G F S+ F + A E R+RGL +++L+Q+IGW+D TN G +R++ D VQ G +IG+ T +AS + A W+L LV +P+L++ST A +A E +A K+ VA E +S++RTV + G + + + +L K + + + G GF + +YA+ +YG L+++ EC D +S+ V S+L G +GQA + ++ A+ AA +I++++ R P ID+S NG + GNV F+ +VLQGL+L++ G+TVALVG SGCGKST IQL++RFYDP +G V L+G DI+ + + +LR ++GIV QEP+LF TIA NI YG R D+ IE A ++AN + FIQ LP Y T+VGERG Q+SGGQKQR+AIARAL+RNP ILLLDEATSALDS+SE VVQ ALD AR GRT I +AHR LSTI+ AD I+ + G ++E GTH EL+ G+Y+ L Q
Sbjct: 697 EKEEMMDAVSLAGSHPLGRHNSVRSARLSVA---SSAVSAQSEDID----------------VSLMDIMRM-NRKEWHFIVVGVIGSAIVGLSTPVFAILFSEV---------------------------LGVLTPGGSAEEQ--AEKRAQGNFYALM--FLILGIVVGFSAFAQSFSF----SIAGESLTSRLRGLTFQAILKQEIGWFDRKTNSVGALCARLSGDAASVQGATGSRIGVLFQAVTTMIASTVLALYFQWKLGLVALCFVPLLLVSTYFQAKIIMGQSALEREALQKSAKVAMEAISNIRTVASLGKERQFHTIYMESLRGPHKEALKKSWIRGFIFGFASSIPMFAYAVTMYYGGWLVVN----------EC---LDFTSVFKVSESLLFGTQMIGQAVAFAPNYNKAKVAANRIFALLRRVPQIDASSNNGLVLENVDGNVNFEQVRFRYPTRKDAEVLQGLSLAVRAGQTVALVGHSGCGKSTCIQLLERFYDPDSGQVQLDGQDINPVNISSLRSQMGIVSQEPILFNLTIAQNIAYGDNSRVVPMDEIIE-AARKANIHVFIQSLPNGYETMVGERGTQLSGGQKQRVAIARALIRNPKILLLDEATSALDSESEHVVQMALDAAREGRTCITIAHR---------------------LSTIQNADNIIVINHGTISEQGTHEELIKLGGLYFELCSVQ 1322
BLAST of P-glycoprotein vs. nr
Match: gi|1067077743|ref|XP_018022501.1| (PREDICTED: multidrug resistance protein 1-like [Hyalella azteca]) HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0 Identity = 384/784 (48.98%), Postives = 516/784 (65.82%), Query Frame = 0
Query: 8 AEEPES-FNSNSSCPSLDIGSHKSPRLASDTVPWTENGSNSGKDPDK--------DEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDTNQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSSDMNGDLPAPGAGNVTFK------------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYGREGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSLVMAQTKKEETRDG-EDEIDEIQDLETVDQ-------EISEMGTA---GPRNRSQSERRPSVASSVFS 759
AEEP+ N S S + HK A T + +G++ K P K E E +E+ KE+ PPV VF+LFR+++ + LI LAL AA + G MP M ILFGD+ N FV +D N+T + +L++++ T + + +++FF + RF G A+IG V V Y+FVTCLN+AAE QV+RIR FL+ LRQ++GW+DT++T DFAS++TEDLNK+QEGMGEK+GM +FF F+A +I AFVHGWELTLVI SV P+L I+ +I+ Q+ LT +E+ Y KAGS+AEEVLS ++TV+AFGG++KEV R+E NL+ AR AG+ RGM +G+ G +WL+IY SY++AFWYG LI+D Y N D YD + LIVVFFSVL+GA+N+GQ P++EAF++ARGAAA +Y +++R AID +G P GN+ F+ VLQGL+L I GETVALVG SGCGKST IQL+QRFYDP G V L+ +D+ L + LR++IG+VGQEPVLF TI +NIRYGR+GI+ +D+ A K+ANA+ F+ LPK+Y T VG+RGAQMSGGQKQR+AIARALV+ P +LLLDEATSALD+QSE++VQ+ALDKAR GRTTI+VAHR L+TIR+AD IVA+KDGR EMGTH ELM ++G+Y+SLV AQ E + E+++ E + +T + +IS++ + P RS S++ S SV S
Sbjct: 46 AEEPKPCINGTSESISPE---HK----ADHTAVFVSDGAHPDKKPPKWRPFKMKEKEKSENKEEEKEEMKPPVGVFQLFRYSTCTERALIILALAAASLAGLCMPAMTILFGDITNAFVYSDFNLTVVA----------YELNMTVEEANTTFQAM----YSDDFFSDVIRFGAGNAIIGGVQLVTGYIFVTCLNYAAEGQVHRIRTQFLRCTLRQEMGWFDTHKTNDFASKVTEDLNKLQEGMGEKVGMIVFFFVNFIAHVIIAFVHGWELTLVIMSVFPLLAITAGLISKIQSSLTTQEMAEYAKAGSIAEEVLSGIKTVLAFGGETKEVKRYESNLVHARNAGVRRGMFSGISLGLVWLIIYASYSIAFWYGTGLILDSR---YPN----DGDYDPAVLIVVFFSVLVGALNMGQVAPFVEAFNIARGAAANVYDILERPSAIDPMSEDGLRPNEVHGNIEFRRVHFEYPSRSDVPVLQGLSLRIQPGETVALVGSSGCGKSTCIQLLQRFYDPRQGEVTLDSVDVRSLNIGWLRDQIGVVGQEPVLFATTIEENIRYGRDGISFEDVVAAAKQANAHDFVMKLPKQYQTQVGDRGAQMSGGQKQRLAIARALVKRPKVLLLDEATSALDNQSESIVQNALDKARQGRTTIIVAHR---------------------LTTIRSADVIVALKDGRAEEMGTHDELMAKRGLYHSLVTAQLAPSERKSALEEDLTEQSEYKTATELDRDVLDDISDITASLAMSPTRRSISKKLGSSTVSVRS 780 HSP 2 Score: 339.347 bits (869), Expect = 8.226e-97 Identity = 235/694 (33.86%), Postives = 351/694 (50.58%), Query Frame = 0
Query: 30 SPRLASDTVPWTENGS---NSGKDPDKDEAEECQEKVKEKSLPPVSVFKLFRFASPRDLFLICLALLAALICGCSMPLMIILFGDLANTFVQNDLNMTQICQGVPFCCDDFLQLDLSLPGCEVTEEDIGHFILNNNFFEQITRFAKGTALIGLVNFVMSYVFVTCLNHAAECQVYRIRGLFLKSVLRQDIGWYDT--NQTGDFASRMTEDLNKVQEGMGEKIGMFIFFATIFVASLINAFVHGWELTLVIFSVMPVLVISTAIIAGSQTYLTARELKAYGKAGSVAEEVLSSVRTVVAFGGQSKEVGRFEHNLIFARKAGIMRGMLTGVGGGFMWLVIYCSYALAFWYGVKLIMDDTEQCYENLAECDPRYDASSLIVVFFSVLMGAMNVGQATPYIEAFSMARGAAAQIYSVIDRTPAIDSS-----DMNGDLPAPGAGNVTFK-------DVLQGLTLSINKGETVALVGPSGCGKSTVIQLVQRFYDPHAGGVFLNGIDISKLQLKALRERIGIVGQEPVLFGCTIADNIRYG---REGITDKDIEMACKEANAYSFIQGLPKKYNTLVGERGAQMSGGQKQRIAIARALVRNPDILLLDEATSALDSQSEAVVQSALDKARHGRTTIVVAHRWVPFPSFDRWIMNTLTNLSYSLSTIRTADKIVAVKDGRVAEMGTHSELMNQKGVYYSL 703
S +L S TV G+ + G + + ++K K +LP + K + P+ I L ++ ++I G PL +LFGD+ T L ++ P E NF+ + IG+V + +++ + E R+R +L Q++GW+D N G SR++ D VQ G ++G + T SL A + W L LV +PV++++ + + + + E + AG +A E +S+VRTV + + R+ L + + L GV GF V + +YA+ +YG L+ D YEN+ + V +++ G M VGQA + ++ + AAA+I+ ++ R P IDSS +N + +V F+ +LQGL+L ++KG+T+ALVG SGCGKSTVI L++RFYDP G + +N D+ L + ++R +G+V QEP+LF +I +NI YG RE D +I A K+AN SFI+ LP+KYNT VG +G Q+SGGQKQRIAIARALVRNP +L+LDEATSALD++SEAVVQ ALDKA+ GRT+IV+AHR LSTI+ AD I + G++ E GTH +LM + G Y L
Sbjct: 768 SKKLGSSTVSVRSQGTQRRSRGSVYIRLPLPQGKKKEKPPTLPMREILKANKTEWPQ----ITLGVVGSVIMGSCTPLYAVLFGDVLGT------------------------LSIADPAEARAEA---------NFYALLF------LCIGIVAAISTFMQAYFFALSGEILTMRLRSRAFSQMLSQELGWFDMDENSVGALCSRLSSDAAAVQGATGSRLGTIMQALTTLGLSLGLALYYDWRLGLVCVPFIPVVLVAVYLESRILSGQSITESEVLQSAGKIAMEAISNVRTVASLHKEEHFSQRYAEALHEPHRLALRNSHLRGVAFGFAQCVPFGAYAVVMFYGGYLV-DKGAMPYENVFK------------VAEAIIFGTMMVGQAVAFAPNYAKGKVAAARIFKLLARVPLIDSSPSVGRTLNEEFDEVRLHDVHFRYPNREDVQILQGLSLVVSKGKTLALVGSSGCGKSTVISLIERFYDPEKGKLTINNEDLKGLNVGSVRRGVGLVSQEPLLFDLSIRENIAYGDNSREVPFD-EIVAAAKQANISSFIESLPEKYNTRVGSKGTQLSGGQKQRIAIARALVRNPSVLMLDEATSALDTESEAVVQEALDKAQAGRTSIVIAHR---------------------LSTIQGADTIAVISSGKLVESGTHDQLMARNGHYAQL 1383 The following BLAST results are available for this feature:
BLAST of P-glycoprotein vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 20
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BLAST of P-glycoprotein vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of P-glycoprotein vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold344_size201325:12627..17492- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold344_size201325-processed-gene-0.9 ID=snap_masked-scaffold344_size201325-processed-gene-0.9|Name=P-glycoprotein|organism=Tigriopus kingsejongensis|type=gene|length=4866bp|location=Sequence derived from alignment at scaffold344_size201325:12627..17492- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'P-glycoprotein' has the following synonyms
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