BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000007906 (supercontig:LSalAtl2s:LSalAtl2s467:197488:204486:1 gene:EMLSAG00000007906 transcript:EMLSAT00000007906 description:"maker-LSalAtl2s467-augustus-gene-2.7")

HSP 1 Score: 352.443 bits (903), Expect = 1.973e-113
Identity = 180/336 (53.57%), Postives = 228/336 (67.86%), Query Frame = 0

Query:   18 LVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQ------QNITTPLLQSP-------VGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            L  +L   TF ++Y LTGD LG G+Y  V TC+N++TD EYAVKII K     SR +V KEIE+++ C G  NIIQLIE+FEE + F LIFEK +GGQLL  I+ RV FTE EA+ ++RDLA A+QFLH +GIAHRDLKPENVLC+  +   P+K+CDFDL SG++        ++TP L +P       VGSAE+MAPEVV AF      D + +L YDKKCD+WSLG+I YILL GY PF +G C   CGW  GE C++C   LF SI NG   F +  W  IS EA+DLIS+LLV+DA  R+  + +L HP+I  GGS+ PL TP ++RR  S
Sbjct:   66 LKSTLVATTFDELYRLTGDFLGSGAYACVRTCVNIWTDIEYAVKIIEKIPGN-SRNRVFKEIEMFHQCLGHDNIIQLIEYFEETDRFCLIFEKIHGGQLLDHIESRVKFTEQEASYVMRDLASAVQFLHKKGIAHRDLKPENVLCVYKDQLCPVKICDFDLGSGIKFTSQYSSPVSTPALLTPVXILSHFVGSAEFMAPEVVEAFMD----DTERDLAYDKKCDLWSLGVIMYILLSGYPPF-SGNCGAQCGWNLGESCNRCQELLFISIQNGIFEFPDSEWMDISMEAKDLISKLLVKDARQRISAEMVLTHPWIKYGGSTNPLGTPQIIRRNNS 395          

HSP 2 Score: 332.413 bits (851), Expect = 1.134e-105
Identity = 174/347 (50.14%), Postives = 227/347 (65.42%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNA------STKISTPKLQTP-------VGSLEYMAPEV------SPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESENHLVR 668
            Y+LT + LG G+ + V T +N       AVK++ K  G  SR+ + +EIE FH C GH NI+QL+E++EE   F L+FEK+ GGQLLDHI+ R  FTE+EA+ ++RDLA A+QFLHKKGIAHRDLKPENVLC+  D + PVK+CDFDL S        S+ +STP L TP       VGS E+MAPEV        ER  +Y+KKCDLWSLGVIM+ILLSG PPFSG+CG +CGW+ GE+C  CQ  L  +IQ G        W +IS  AKDL+  LL K+  +RI    +L HPWI   G+++ LGTP+++RR+ S + LS +A S  A+N+      S N + R
Sbjct:   79 YRLTGDFLGSGAYACVRTCVNIWTDIEYAVKIIEKIPGN-SRNRVFKEIEMFHQCLGHDNIIQLIEYFEETDRFCLIFEKIHGGQLLDHIESRVKFTEQEASYVMRDLASAVQFLHKKGIAHRDLKPENVLCVYKDQLCPVKICDFDLGSGIKFTSQYSSPVSTPALLTPVXILSHFVGSAEFMAPEVVEAFMDDTERDLAYDKKCDLWSLGVIMYILLSGYPPFSGNCGAQCGWNLGESCNRCQELLFISIQNGIFEFPDSEWMDISMEAKDLISKLLVKDARQRISAEMVLTHPWIKYGGSTNPLGTPQIIRRNNSARELSMFAESAMAVNRVVLQHMSLNRMDR 424          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000006973 (supercontig:LSalAtl2s:LSalAtl2s397:1143346:1147158:-1 gene:EMLSAG00000006973 transcript:EMLSAT00000006973 description:"maker-LSalAtl2s397-snap-gene-11.19")

HSP 1 Score: 339.347 bits (869), Expect = 5.486e-110
Identity = 176/314 (56.05%), Postives = 224/314 (71.34%), Query Frame = 0

Query:   18 LVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRN-WCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAF--------NTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQD-CGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIV 321
            L+ SLS NTF D+Y LTGDVLGEGS+G+V T IN FT  E A K ISK N W FSR KVL+E+ELYYLC+G P I+QL+E+FEE + F+LIFEKA GG L S +Q +   +E +A SII+DL++AL FLH  GIAHRDLKPENVLC++  SP P+KLCDFDLCS V  +++TP L SPVGSAEYMAPEVV+AF             + +DDEL YDK CD+WSLG++ YIL+ G +PF  G C+ D C W+ G EC++C R+LF +I +G L F E+    IS EA+DLI  LL ++   R  T+ I  HP+++
Sbjct:   65 LISSLSLNTFPDVYSLTGDVLGEGSFGKVMTGINNFTGEEVAXKRISKTNSWFFSRDKVLREVELYYLCRGVPEIVQLLEYFEEEDAFYLIFEKAQGGPLFSYLQSKEPLSENQARSIIKDLSKALSFLHKNGIAHRDLKPENVLCINKGSPTPVKLCDFDLCSSVIPSVSTPRLTSPVGSAEYMAPEVVNAFIYSSLDEYEYYEEYMDDDELYYDKNCDLWSLGVLTYILITGIMPF-RGSCNDDMCDWDSGGECAECQRTLFHAIKSGNLRFPEN--KPISKEAKDLIMSLLNKEPRKRPTTEQIATHPWVL 375          

HSP 2 Score: 275.789 bits (704), Expect = 1.136e-85
Identity = 150/345 (43.48%), Postives = 205/345 (59.42%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAK-NTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRS------------------YNKKCDLWSLGVIMHILLSGSPPFSGDCGQE-CGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESENH 665
            Y LT + LG+GS  +V+T IN   G+ +A K ++K N+  FSR  +LRE+E ++LC G   I+QLLE++EE   FYL+FEK +GG L  +++ +E  +E +A  II+DL++AL FLHK GIAHRDLKPENVLC+   S  PVKLCDFDL S+    +STP+L +PVGS EYMAPEV      S                  Y+K CDLWSLGV+ +IL++G  PF G C  + C WD+G  C  CQ  L  AI+ G  NL  P    IS+ AKDL+++LL K   +R  T  +  HPW+    T+    T  V  + +  +G+    TS+        + E +NH
Sbjct:   78 YSLTGDVLGEGSFGKVMTGINNFTGEEVAXKRISKTNSWFFSRDKVLREVELYYLCRGVPEIVQLLEYFEEEDAFYLIFEKAQGGPLFSYLQSKEPLSENQARSIIKDLSKALSFLHKNGIAHRDLKPENVLCINKGSPTPVKLCDFDLCSSVIPSVSTPRLTSPVGSAEYMAPEVVNAFIYSSLDEYEYYEEYMDDDELYYDKNCDLWSLGVLTYILITGIMPFRGSCNDDMCDWDSGGECAECQRTLFHAIKSG--NLRFPENKPISKEAKDLIMSLLNKEPRKRPTTEQIATHPWVLGISTT----TTTVHNKKSFFEGMKRTQTSSML------YPEDDNH 410          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000006998 (supercontig:LSalAtl2s:LSalAtl2s397:959218:996753:-1 gene:EMLSAG00000006998 transcript:EMLSAT00000006998 description:"maker-LSalAtl2s397-augustus-gene-10.4")

HSP 1 Score: 204.142 bits (518), Expect = 2.174e-61
Identity = 106/180 (58.89%), Postives = 129/180 (71.67%), Query Frame = 0

Query:  340 SYQLTEEQLGQGSSSQVVTAINRSN-----GQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPE 514
            +Y LTE  LG GS  +V    +RS+     G+  AVK+V +++  FSR  +LREIETF++C  H NI+Q L FYEE   FYLVFEK+EGG L DHI+ R    E EA++I+ +LA+AL FLH+KGIAHRDLK ENVLC+R DS  PVKL DFDL S   T +STPKL TPVGSLEYMAPE
Sbjct:   26 NYVLTETLLGSGSYGEVRICKSRSDNQNEEGKEFAVKIVKESSVFFSRVRVLREIETFYICREHENIIQFLHFYEEPLGFYLVFEKIEGGPLFDHIRNRNYLNEAEASQIVLELAKALSFLHQKGIAHRDLKAENVLCVRVDSPCPVKLGDFDLCSKL-TNLSTPKLLTPVGSLEYMAPE 204          

HSP 2 Score: 191.43 bits (485), Expect = 1.086e-56
Identity = 98/199 (49.25%), Postives = 134/199 (67.34%), Query Frame = 0

Query:   12 RKKKKRLVESLSCN-TFKDIYYLTGDVLGEGSYGRVETCINMFTD-----TEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPE 204
            + + KR  ++L+   TF + Y LT  +LG GSYG V  C +   +      E+AVKI+ + +  FSR +VL+EIE +Y+C+   NIIQ + F+EEP  F+L+FEK  GG L   I+ R +  E EA+ I+ +LA+AL FLH +GIAHRDLK ENVLC+  +SP P+KL DFDLCS +  N++TP L +PVGS EYMAPE
Sbjct:    7 KPRSKRRFQNLNLRLTFTENYVLTETLLGSGSYGEVRICKSRSDNQNEEGKEFAVKIVKESSVFFSRVRVLREIETFYICREHENIIQFLHFYEEPLGFYLVFEKIEGGPLFDHIRNRNYLNEAEASQIVLELAKALSFLHQKGIAHRDLKAENVLCVRVDSPCPVKLGDFDLCSKL-TNLSTPKLLTPVGSLEYMAPE 204          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000004239 (supercontig:LSalAtl2s:LSalAtl2s222:1379053:1391929:1 gene:EMLSAG00000004239 transcript:EMLSAT00000004239 description:"maker-LSalAtl2s222-augustus-gene-13.20")

HSP 1 Score: 162.155 bits (409), Expect = 8.367e-42
Identity = 99/309 (32.04%), Postives = 166/309 (53.72%), Query Frame = 0

Query:  344 TEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCL-RPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTF 651
             +E +G GS S+    I+++     A+KV+ K+     R     E+E       H NI+ L + +E+    YL+FE M GG+LLD I ++E+F+E+EA  I+  L  A+ +LH+ G+ HRDLKP N+L   +    + +++CDF  A     +     L TP  +  Y+APEV   +++ Y+  CD+WSLGV++HI+LSG  PF+         D+ E  L       + I+EG ++     W ++S  AK+L+ ++ + +  +R     +L HPW+TS+  S       + R+ +S+  L    ++TF
Sbjct:  439 VKELIGVGSYSECKRCIHKATNVEYAMKVIDKS----KRDCNSDEVEILLRYGQHPNIISLKDVFEDKDFAYLIFEYMRGGELLDKILQQEIFSEREARSIMERLTSAVHYLHQNGVVHRDLKPANILYADKFGDPNTLRICDFGFAKQ--LRADNGLLMTPCYTANYVAPEVL--KQQGYDAACDVWSLGVLLHIMLSGKAPFANGPN-----DSMEVIL-------SRIEEGKIDTSSGNWLSVSTNAKNLVRSMFDIDPRKRPTALEVLQHPWMTSKSPSD----ANISRQISSSSTLKGAVSATF 723          

HSP 2 Score: 158.688 bits (400), Expect = 9.787e-41
Identity = 107/336 (31.85%), Postives = 166/336 (49.40%), Query Frame = 0

Query:   32 YLTGDVLGEGSYGRVETCINMFTDTEYAVKII--SKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSA-NSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTSYQLTEEQLGQGSSSQVVTAI-NRS 363
            Y   +++G GSY   + CI+  T+ EYA+K+I  SKR+ C S      E+E+       PNII L + FE+ ++ +LIFE   GG+LL +I ++  F+E EA SI+  L  A+ +LH  G+ HRDLKP N+L       P  +++CDF     ++ +    LL +P  +A Y+APEV+             +  YD  CD+WSLG++ +I+L G  PFA G  D                 +   I  G +      W S+S  A++L+  +   D   R     +L+HP++    S +P +         S Q++     +G+ S    AI NRS
Sbjct:  437 YEVKELIGVGSYSECKRCIHKATNVEYAMKVIDKSKRD-CNS-----DEVEILLRYGQHPNIISLKDVFEDKDFAYLIFEYMRGGELLDKILQQEIFSEREARSIMERLTSAVHYLHQNGVVHRDLKPANILYADKFGDPNTLRICDFGFAKQLRAD--NGLLMTPCYTANYVAPEVLK------------QQGYDAACDVWSLGVLLHIMLSGKAPFANGPND-------------SMEVILSRIEEGKIDTSSGNWLSVSTNAKNLVRSMFDIDPRKRPTALEVLQHPWMT---SKSPSDA------NISRQISSSSTLKGAVSATFMAIRNRS 730          

HSP 3 Score: 99.7525 bits (247), Expect = 7.050e-22
Identity = 94/326 (28.83%), Postives = 150/326 (46.01%), Query Frame = 0

Query:   37 VLGEGSYGRV---ETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQ-PNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVV----HAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKA-----ILEHPF--------IVNGGSSAPLETPSMLRRQTSY 341
            VLG+GS+G+V          + T YA+K++ K    F R +V  + E   L + + P +++L   F+     +LI +   GG L +++ + + FTE +    + +LA AL  LH  GI +RDLKPEN+L L +N    I + DF    G+ +        S  G+ EYMAPE+V    H+F                  D WS GI+ Y +L G LPF         G  + E  S+        I    +   E+    +S EAQ L+  L  R+ + RL +       +++HPF        ++   +S P + P+++  +T Y
Sbjct:   87 VLGQGSFGKVFLVRKIEGKDSGTLYAMKVLKKATLRF-RDRVRTKKERDILAEIEHPFMVKLYYAFQTEGKLYLILDFLRGGDLFTRLSKEIMFTEEDVKFYLAELALALIHLHSLGIIYRDLKPENLL-LDSNG--HIAVTDF----GLSKETIEDKAYSFCGTVEYMAPEIVNRKGHSF----------------AADWWSFGILMYEMLTGQLPFH--------GENRKETMSQ--------ILKAKIGMPEY----LSPEAQSLLRVLFKRNPAHRLGSGPSGKDDVMKHPFFASIKWSELIEKKTSPPFK-PAIVADETYY 367          

HSP 4 Score: 98.2117 bits (243), Expect = 2.569e-21
Identity = 71/217 (32.72%), Postives = 112/217 (51.61%), Query Frame = 0

Query:  348 LGQGSSSQV--VTAI-NRSNGQALAVKVVAKNTGMF-------SRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQEC 554
            LGQGS  +V  V  I  + +G   A+KV+ K T  F          ++L EIE       H  +++L   ++     YL+ + + GG L   + +  +FTE++    + +LA AL  LH  GI +RDLKPEN+L    DS   + + DF L    S +    K  +  G++EYMAPE+    R+ ++   D WS G++M+ +L+G  PF G+  +E 
Sbjct:   88 LGQGSFGKVFLVRKIEGKDSGTLYAMKVLKKATLRFRDRVRTKKERDILAEIE-------HPFMVKLYYAFQTEGKLYLILDFLRGGDLFTRLSKEIMFTEEDVKFYLAELALALIHLHSLGIIYRDLKPENLLL---DSNGHIAVTDFGL----SKETIEDKAYSFCGTVEYMAPEIV--NRKGHSFAADWWSFGILMYEMLTGQLPFHGENRKET 288          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000001966 (supercontig:LSalAtl2s:LSalAtl2s134:758246:759645:-1 gene:EMLSAG00000001966 transcript:EMLSAT00000001966 description:"snap_masked-LSalAtl2s134-processed-gene-7.20")

HSP 1 Score: 149.828 bits (377), Expect = 1.181e-39
Identity = 94/298 (31.54%), Postives = 151/298 (50.67%), Query Frame = 0

Query:   29 DIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLS-ANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGS 325
            +I Y  G+ +G+G++  V+ C +  T   YA+K+I K         +  EI +  +     NII+L+E F+ P+  +L+ E   GG L   I     ++E E+  ++ DL  AL++LH + I HRD+KPEN+L    ++    +K+ DF    G+ Q +  PL     G+  Y+APE++             E  Y  K DIW+ G+I YILLCG+ PF++   +Q+               LFD I +G   F+   W  IS+ A++LIS +L  D   R     IL+H ++++  S
Sbjct:  109 EIKYDIGEKIGDGNFAVVKKCTSRKTGKCYALKVIDKGKXQGKEHMIESEITILNVV-SHTNIIELLEVFDTPDERYLVTEYVSGGDLFDAITLDTKYSEDESRIMVNDLTSALKYLHDKMIVHRDIKPENLLVFDRSDGCKSLKIGDF----GLAQAVNEPLFIV-CGTPTYVAPEILA------------ETGYGXKVDIWATGVIMYILLCGFPPFSSSTNNQE--------------ELFDQILSGLFEFNSPNWDDISFPAKELISWMLQIDPLQRYSAYEILQHKWMLSTPS 374          

HSP 2 Score: 145.206 bits (365), Expect = 4.956e-38
Identity = 94/277 (33.94%), Postives = 140/277 (50.54%), Query Frame = 0

Query:  346 EQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCL-RPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITS 621
            E++G G+ + V    +R  G+  A+KV+ K         +  EI   ++   H NI++LLE ++     YLV E + GG L D I     ++E E+  ++ DL  AL++LH K I HRD+KPEN+L   R D    +K+ DF LA      ++ P L    G+  Y+APE+  E    Y  K D+W+ GVIM+ILL G PPFS                  Q +L   I  G    + P W +IS PAK+L+  +L+ +  +R     +L H W+ S
Sbjct:  116 EKIGDGNFAVVKKCTSRKTGKCYALKVIDKGKXQGKEHMIESEITILNVV-SHTNIIELLEVFDTPDERYLVTEYVSGGDLFDAITLDTKYSEDESRIMVNDLTSALKYLHDKMIVHRDIKPENLLVFDRSDGCKSLKIGDFGLAQ----AVNEP-LFIVCGTPTYVAPEILAE--TGYGXKVDIWATGVIMYILLCGFPPFSSSTNN-------------QEELFDQILSGLFEFNSPNWDDISFPAKELISWMLQIDPLQRYSAYEILQHKWMLS 371          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000013013 (supercontig:LSalAtl2s:LSalAtl2s995:1074:17118:1 gene:EMLSAG00000013013 transcript:EMLSAT00000013013 description:"maker-LSalAtl2s995-augustus-gene-0.26")

HSP 1 Score: 142.51 bits (358), Expect = 2.352e-38
Identity = 79/212 (37.26%), Postives = 120/212 (56.60%), Query Frame = 0

Query:  414 FYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEV-SPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGT 624
             Y+V   M+GG+L   I+E++ F E+EA+ +++D+  A+++LH   IAHRDLKPEN+L    +S   +KL DF  A    T+ +   L+TP  +  Y+APEV  PE+   Y+K CD+WSLGVIM+ILL G PPF  + G                 +   I+ G     +P W N+SQ AKDL+  +L  + ++R+    ++ + WI    T
Sbjct:    1 MYIVI--MQGGELFQRIQEKQAFNEREASGLMKDICIAIKYLHDMNIAHRDLKPENLLYTDKESNSILKLTDFGFAKETLTRDT---LETPCYTPYYVAPEVLGPEK---YDKSCDIWSLGVIMYILLCGFPPFYSNHGH-----------AISPGMKKRIRSGQYEYPEPEWKNVSQDAKDLIRQMLNTDPEKRLTIDQVIKNTWIAQYNT 193          

HSP 2 Score: 129.028 bits (323), Expect = 1.397e-33
Identity = 78/230 (33.91%), Postives = 120/230 (52.17%), Query Frame = 0

Query:  113 GGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIV--NGGSSAPLETPSMLRRQTS 340
            GG+L  +IQ +  F E EA+ +++D+  A+++LH   IAHRDLKPEN+L     S   +KL DF      ++ +T   L++P  +  Y+APEV+                YDK CDIWSLG+I YILLCG+ PF +           G   S   +     I +G   + E  W ++S +A+DLI ++L  D   RL    ++++ +I   N     PL T  +L+ +T+
Sbjct:    8 GGELFQRIQEKQAFNEREASGLMKDICIAIKYLHDMNIAHRDLKPENLLYTDKESNSILKLTDFGFA---KETLTRDTLETPCYTPYYVAPEVLGPEK------------YDKSCDIWSLGVIMYILLCGFPPFYSN---------HGHAISPGMKK---RIRSGQYEYPEPEWKNVSQDAKDLIRQMLNTDPEKRLTIDQVIKNTWIAQYNTVPQTPLLTCDVLKEETA 210          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000011362 (supercontig:LSalAtl2s:LSalAtl2s784:66732:102413:-1 gene:EMLSAG00000011362 transcript:EMLSAT00000011362 description:"augustus_masked-LSalAtl2s784-processed-gene-1.0")

HSP 1 Score: 147.902 bits (372), Expect = 4.729e-38
Identity = 102/329 (31.00%), Postives = 159/329 (48.33%), Query Frame = 0

Query:  340 SYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEG--HANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTP----VGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSE----GTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFS 658
            +Y+L EE LG+G+ S V   + +S G   A K++  NT   S  +  +      +C    H NI++L +  +E    YLVF+ + GG+L + I  RE ++E +A+  I+ +  ++   H  G+ HRDLKPEN+L         VKL DF LA      I     Q       G+  Y++PEV   ++  Y K  D+W+ GVI++ILL G PPF         WD        Q +L   I+ G+ +   P W  ++  AK+L+  +L  N  +RI +   L HPWI          HR  T   L++  + + L     +T  +++ FS
Sbjct:   12 NYELKEE-LGKGAFSVVRRCVQKSTGLDFASKII--NTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHSNGVVHRDLKPENLLLASKQKGAAVKLADFGLA------IEVQGDQHAWFGFAGTPGYLSPEVL--KKEPYGKAVDIWACGVILYILLVGYPPF---------WDED------QHRLYAQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPAKRIRSDEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLVSRNFS 314          

HSP 2 Score: 134.035 bits (336), Expect = 2.080e-33
Identity = 88/295 (29.83%), Postives = 147/295 (49.83%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKII-SKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFI 320
            F D Y L  + LG+G++  V  C+   T  ++A KII +K+       K+ +E  +    Q  PNI++L +  +E  Y +L+F+   GG+L   I  R  ++E +A+  I+ + +++   H  G+ HRDLKPEN+L  S      +KL DF L   VQ +          G+  Y++PEV+             +  Y K  DIW+ G+I YILL GY PF          W++ +        L+  I  G   +    W +++ EA++LI+++L  + + R+ +   L+HP+I
Sbjct:    9 FTDNYELKEE-LGKGAFSVVRRCVQKSTGLDFASKIINTKKLSARDFQKLEREARICRKLQ-HPNIVRLHDSIQEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHSNGVVHRDLKPENLLLASKQKGAAVKLADFGLAIEVQGDQHAWF--GFAGTPGYLSPEVLK------------KEPYGKAVDIWACGVILYILLVGYPPF----------WDEDQ------HRLYAQIKAGAYDYPSPEWDTVTPEAKNLINQMLTVNPAKRIRSDEALKHPWI 271          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000011184 (supercontig:LSalAtl2s:LSalAtl2s760:424402:425388:1 gene:EMLSAG00000011184 transcript:EMLSAT00000011184 description:"augustus_masked-LSalAtl2s760-processed-gene-3.6")

HSP 1 Score: 128.257 bits (321), Expect = 1.018e-32
Identity = 94/285 (32.98%), Postives = 147/285 (51.58%), Query Frame = 0

Query:  348 LGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSN----MLREIET-FHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKEREL--FTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTS 625
            LG+G    V  A  + +   +A+KV+ KN     ++N    + REIE   HL   H +IL+L  ++ + T  YL+ E    G+L   +K   L  F E  AAR I  ++ AL +LH K + HRD+KPEN+L    DS   +K+ DF  + +A +     +  T  G+L+Y++PE+     RS+++K DLWSLG++    L G PPF  D  QE                     +   N+D  + + IS  A+DL+  LL+K  +RR+  + +++HPWI    +S
Sbjct:   59 LGKGKFGNVYLAREKKSKFIVALKVMFKN--QLKKANVEHQLRREIEIQXHLR--HPHILRLYGYFYDETRVYLILEFASKGELYKLLKNEPLKRFEETLAARYIYQMSGALDYLHSKKVIHRDIKPENLLL---DSKGNLKIADFGWSVHAPSS----RRGTMCGTLDYLSPEMI--EGRSHDEKVDLWSLGILCFEFLVGKPPFETDNQQET-------------------YKKITNVDIEWPSFISPEARDLISKLLQKIPERRLPLKEVIVHPWIMKYRSS 311          

HSP 2 Score: 97.8265 bits (242), Expect = 1.893e-22
Identity = 81/296 (27.36%), Postives = 138/296 (46.62%), Query Frame = 0

Query:   35 GDVLGEGSYGRVETCINMFTDTEYAVKIISKRNW--CFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRR--VHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSS 326
            G  LG+G +G V       +    A+K++ K          ++ +EIE+    +  P+I++L  +F +    +LI E A  G+L   ++      F E  AA  I  ++ AL +LH + + HRD+KPEN+L  S  +   +K+ DF    G   +  +    +  G+ +Y++PE++               ++D+K D+WSLGI+ +  L G  PF                 +   +  +  I N  +     + S IS EA+DLIS+LL +    RL  K ++ HP+I+   SS
Sbjct:   56 GRPLGKGKFGNVYLAREKKSKFIVALKVMFKNQLKKANVEHQLRREIEIQXHLR-HPHILRLYGYFYDETRVYLILEFASKGELYKLLKNEPLKRFEETLAARYIYQMSGALDYLHSKKVIHRDIKPENLLLDSKGN---LKIADF----GWSVHAPSSRRGTMCGTLDYLSPEMIEG------------RSHDEKVDLWSLGILCFEFLVGKPPFE----------------TDNQQETYKKITNVDI----EWPSFISPEARDLISKLLQKIPERRLPLKEVIVHPWIMKYRSS 311          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000012225 (supercontig:LSalAtl2s:LSalAtl2s88:337425:490616:1 gene:EMLSAG00000012225 transcript:EMLSAT00000012225 description:"augustus_masked-LSalAtl2s88-processed-gene-5.0")

HSP 1 Score: 125.946 bits (315), Expect = 1.999e-32
Identity = 92/265 (34.72%), Postives = 135/265 (50.94%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLRE---IETFHLCE-GHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKIST-PKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLL 600
            Y  T E++G G  + V   + +S     A K + K     SR  + RE    E   L E  HANI+ L + YE  T   LV E + GG+L D I E+E  +E+EA+  I+ +   ++ +H K I+H DLKPEN++ L   S   +KL DF L    S KI    +++  +G+ E+++PEV      S N   D+WS+GVI +ILLSG+ PF G+  QE  ++N  AC                  D  F++  S+ AKD +  L 
Sbjct:   25 YFETFEEIGSGQFAIVRRVVEKSTSTEYAGKFIKKKRLETSRRGVAREDIQKEIHILAEMEHANIIYLHQVYESQTQVILVLELLRGGELFDFISEKERLSEEEASNFIKQILLGVRHMHTKCISHLDLKPENIMLLGKHS-QLLKLIDFGL----SRKIHPGNEIREMLGTPEFVSPEVVNYEPLSLN--TDMWSIGVITYILLSGASPFLGETQQET-FENIVAC--------------DYEFDSEFFSQTSELAKDFIRRLF 267          

HSP 2 Score: 116.316 bits (290), Expect = 3.261e-29
Identity = 88/301 (29.24%), Postives = 146/301 (48.50%), Query Frame = 0

Query:   17 RLVESLSCNTFK----DIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKE---IELYYLCQGQ-PNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVH----AFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDA 305
            +LV++++   F+    + Y+ T + +G G +  V   +   T TEYA K I K+    SR  V +E    E++ L + +  NII L + +E      L+ E   GG+L   I  +   +E EA++ I+ +   ++ +H + I+H DLKPEN++ L  +S L +KL DF L   +        ++  +G+ E+++PEVV+    + NT                D+WS+G+I YILL G  PF  G+  Q+                F++I      F   ++S  S  A+D I RL V D+
Sbjct:    7 KLVQTINSTKFRTEPFEDYFETFEEIGSGQFAIVRRVVEKSTSTEYAGKFIKKKRLETSRRGVAREDIQKEIHILAEMEHANIIYLHQVYESQTQVILVLELLRGGELFDFISEKERLSEEEASNFIKQILLGVRHMHTKCISHLDLKPENIMLLGKHSQL-LKLIDFGLSRKIHPG---NEIREMLGTPEFVSPEVVNYEPLSLNT----------------DMWSIGVITYILLSGASPF-LGETQQET---------------FENIVACDYEFDSEFFSQTSELAKDFIRRLFVVDS 271          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. L. salmonis genes
Match: EMLSAG00000003715 (supercontig:LSalAtl2s:LSalAtl2s1:3290800:3325247:1 gene:EMLSAG00000003715 transcript:EMLSAT00000003715 description:"maker-LSalAtl2s1-augustus-gene-33.19")

HSP 1 Score: 129.028 bits (323), Expect = 9.488e-32
Identity = 91/283 (32.16%), Postives = 136/283 (48.06%), Query Frame = 0

Query:  341 YQL-TEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHI--KERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTKISTPKLQTPVGSLEYMAPEVSPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWIT 620
            YQ+ ++E LG G    V   ++R NG+A+AVKV+ K      +   L+   +      H  ++ L   +E     Y+V EK++G  +L+ I   E    +E+    ++  +  AL+ LH K I H DLKPENVL         VKLCDF  A     K      ++ VG+  Y+APEV   R + YN+  D+WS GVI+++ LSG+ PF+ D                   ++  IQ  S       W  I   A DL+ NLL+    +RI     L+HPW++
Sbjct:  190 YQIFSDEILGSGQFGIVYGGVHRQNGEAVAVKVIDKMRFPSKQEAALKNEVSILQNIHHPGVVNLERMFETLERIYVVMEKLKG-DMLEMILSSEHGRLSERVTKFVVTQILVALKHLHSKNIVHCDLKPENVLLSSNSDFPQVKLCDFGFARIIGEK---GFRKSVVGTPAYLAPEVL--RNKGYNRSLDMWSTGVIIYVSLSGTFPFNED-----------------EDINEQIQNASFMYPTHPWKTIDSKAIDLIGNLLQVKSRKRISVDKALLHPWLS 449          

HSP 2 Score: 114.005 bits (284), Expect = 8.117e-27
Identity = 88/299 (29.43%), Postives = 133/299 (44.48%), Query Frame = 0

Query:   26 TFKDIYYLTGD-VLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVH--FTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLP-IKLCDFDLCSGVQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFI 320
            T   +Y +  D +LG G +G V   ++       AVK+I K  +   +   LK           P ++ L   FE  E  +++ EK   G +L  I    H   +E     ++  +  AL+ LH + I H DLKPENVL LS+NS  P +KLCDF     + +       +S VG+  Y+APEV+       G        Y++  D+WS G+I Y+ L G  PF     D+D                 + I N + ++  H W +I  +A DLI  LL   +  R+     L HP++
Sbjct:  185 TLSQLYQIFSDEILGSGQFGIVYGGVHRQNGEAVAVKVIDKMRFPSKQEAALKNEVSILQNIHHPGVVNLERMFETLERIYVVMEK-LKGDMLEMILSSEHGRLSERVTKFVVTQILVALKHLHSKNIVHCDLKPENVL-LSSNSDFPQVKLCDFGFARIIGEK---GFRKSVVGTPAYLAPEVLR----NKG--------YNRSLDMWSTGVIIYVSLSGTFPFNE---DEDIN---------------EQIQNASFMYPTHPWKTIDSKAIDLIGNLLQVKSRKRISVDKALLHPWL 448          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|30315972|sp|O08605.2|MKNK1_MOUSE (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 1; AltName: Full=MAP kinase signal-integrating kinase 1; Short=MAPK signal-integrating kinase 1; Short=Mnk1)

HSP 1 Score: 348.206 bits (892), Expect = 8.379e-112
Identity = 166/326 (50.92%), Postives = 226/326 (69.33%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEVSPERRRS---YNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFS 658
            YQLT E LG+G+ ++V  A+N  +G+  AVK++ K  G  SRS + RE+ET + C+G+ NIL+L+EF+E+ T FYLVFEK++GG +L HI++R+ F E+EA+R++RD+A AL FLH KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S     N+ T I+TP+L TP GS EYMAPEV    R     Y+K+CDLWSLGV+++I+LSG PPF G CG +CGWD GE C  CQ KL  +IQEG        WA+IS  AKDL+  LL ++  +R+    +L HPW+  +     L TP+VL+R++ST  L+ +A    ALN+  S
Sbjct:   48 YQLTSELLGEGAYAKVQGAVNLQSGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNRNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKRKHFNEREASRVVRDVATALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFRDEATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRMCQNKLFESIQEGKYEFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLS 372          

HSP 2 Score: 336.65 bits (862), Expect = 3.178e-107
Identity = 166/329 (50.46%), Postives = 219/329 (66.57%), Query Frame = 0

Query:   17 RLVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            R  +SL    F+D+Y LT ++LGEG+Y +V+  +N+ +  EYAVKII K+    SR +V +E+E  Y CQG  NI++LIEFFE+   F+L+FEK  GG +L+ IQ+R HF E EA+ ++RD+A AL FLH +GIAHRDLKPEN+LC S     P+K+CDFDL SGV+ N     ITTP L +P GSAEYMAPEVV  F        D+   YDK+CD+WSLG++ YI+L GY PF  G C  DCGW++GE C  C   LF+SI  G   F +  W+ IS EA+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   35 RATDSLP-GKFEDVYQLTSELLGEGAYAKVQGAVNLQSGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNRNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKRKHFNEREASRVVRDVATALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFR-------DEATFYDKRCDLWSLGVVLYIMLSGYPPF-VGHCGADCGWDRGEVCRMCQNKLFESIQEGKYEFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSS 353          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|90102031|sp|Q4G050.1|MKNK1_RAT (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 1; AltName: Full=MAP kinase signal-integrating kinase 1; Short=MAPK signal-integrating kinase 1; Short=Mnk1)

HSP 1 Score: 343.969 bits (881), Expect = 2.460e-110
Identity = 165/326 (50.61%), Postives = 225/326 (69.02%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEVSPERRRS---YNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFS 658
            YQLT E LG+G+ ++V  A++  +G+  AVK++ K  G  SRS + RE+ET + C+G+ NIL+L+EF+E+ T FYLVFEK++GG +L HI++R+ F E EA+R++RD+A AL FLH KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S     N+ T I+TP+L TP GS EYMAPEV    R     Y+K+CDLWSLGV+++I+LSG PPF G CG +CGWD GE C  CQ KL  +IQEG        WA+IS  AKDL+  LL ++  +R+    +L HPW+  +     L TP+VL+R++ST  L+ +A    ALN+  S
Sbjct:   36 YQLTSELLGEGAYAKVQGAVSLQSGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNRNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKRKHFNELEASRVVRDVATALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFRDEATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRMCQNKLFESIQEGKYEFPDKDWAHISTEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLS 360          

HSP 2 Score: 335.109 bits (858), Expect = 7.109e-107
Identity = 165/329 (50.15%), Postives = 220/329 (66.87%), Query Frame = 0

Query:   17 RLVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            R  +SL    F+D+Y LT ++LGEG+Y +V+  +++ +  EYAVKII K+    SR +V +E+E  Y CQG  NI++LIEFFE+   F+L+FEK  GG +L+ IQ+R HF E+EA+ ++RD+A AL FLH +GIAHRDLKPEN+LC S     P+K+CDFDL SGV+ N     ITTP L +P GSAEYMAPEVV  F        D+   YDK+CD+WSLG++ YI+L GY PF  G C  DCGW++GE C  C   LF+SI  G   F +  W+ IS EA+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   23 RATDSLP-GKFEDVYQLTSELLGEGAYAKVQGAVSLQSGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNRNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKRKHFNELEASRVVRDVATALDFLHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFR-------DEATFYDKRCDLWSLGVVLYIMLSGYPPF-VGHCGADCGWDRGEVCRMCQNKLFESIQEGKYEFPDKDWAHISTEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSS 341          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|90102030|sp|Q58D94.2|MKNK1_BOVIN (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 1; AltName: Full=MAP kinase signal-integrating kinase 1; Short=MAPK signal-integrating kinase 1; Short=Mnk1)

HSP 1 Score: 340.502 bits (872), Expect = 6.331e-109
Identity = 161/329 (48.94%), Postives = 227/329 (69.00%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSE 661
            Y+LT E LG+G++++V  A++  NG   AVK++ K+ G  SRS + RE+ET + C+G+ +IL+L+EF+E+ T FYLVFEK++GG +L HI++++ F E+EA+R++RD+A AL F H KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S     N+ T I+TP+L TP GS EYMAPEV     +    Y+K+CDLWSLGV+++I+LSG PPF G CG +CGWD GE C  CQ KL  +IQ+G        WA+IS  AKDL+  LL ++  +R+    +L HPW+  +     L TP+VL+R++ST  L+ +A    ALN+  S  E
Sbjct:   36 YKLTSELLGEGANAKVQVAVSLQNGNEYAVKIIEKHAG-HSRSRVFREVETLYQCQGNKHILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFRHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDEATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCTVCQNKLFESIQKGKYEFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHE 363          

HSP 2 Score: 325.865 bits (834), Expect = 2.903e-103
Identity = 158/319 (49.53%), Postives = 213/319 (66.77%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            F+D+Y LT ++LGEG+  +V+  +++    EYAVKII K +   SR +V +E+E  Y CQG  +I++LIEFFE+   F+L+FEK  GG +L+ IQ++ HF E EA+ ++RD+A AL F H +GIAHRDLKPEN+LC S     P+K+CDFDL SGV+ N     ITTP L +P GSAEYMAPEVV  F        D+   YDK+CD+WSLG++ YI+L GY PF  G C  DCGW++GE C+ C   LF+SI  G   F +  W+ IS EA+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   32 FEDVYKLTSELLGEGANAKVQVAVSLQNGNEYAVKIIEK-HAGHSRSRVFREVETLYQCQGNKHILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFRHTKGIAHRDLKPENILCESPEKVSPVKICDFDLGSGVKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFT-------DEATFYDKRCDLWSLGVVLYIMLSGYPPF-VGHCGADCGWDRGEVCTVCQNKLFESIQKGKYEFPDKDWAHISNEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPERGLPTPQVLQRNSS 341          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|82234476|sp|Q66JF3.1|MKNK1_XENTR (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 1; AltName: Full=MAP kinase signal-integrating kinase 1; Short=MAPK signal-integrating kinase 1; Short=Mnk1)

HSP 1 Score: 337.035 bits (863), Expect = 1.287e-107
Identity = 163/331 (49.24%), Postives = 226/331 (68.28%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESE 663
            Y+LT+E LG+G+ ++V   ++  NG+  AVK+V K  G  SRS + RE+ET + C+G+ NIL+L+EF E+   FYLVFEK+ GG +L HI++R+ F E+EA+++++D+A AL FLH KGIAHRDLKPEN+LC   D + PVK+CDFDL S     +A T I+TP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++I+LSG PPF G+CG +CGWD GE C  CQ KL  +IQEG     +  W++IS  AKDL+  LL ++   R+    +L HPW+  +     L TP VL+R++ST+ L+ +A    ALN+  S  +S+
Sbjct:   36 YRLTDELLGEGAYAKVQGCVSLQNGKDYAVKIVEKKAG-HSRSRVFREVETLYQCQGNKNILELIEFCEDDARFYLVFEKLRGGSILSHIQKRKHFNEREASKVVKDIASALDFLHTKGIAHRDLKPENILCEFKDKVSPVKICDFDLGSGVKLNSACTPITTPELTTPCGSAEYMAPEVVEVFTEEATFYDKRCDLWSLGVILYIMLSGYPPFVGNCGADCGWDRGEMCRVCQNKLFESIQEGKYEFPEKDWSHISNSAKDLISKLLVRDAKERLSAAQVLQHPWLQGDAPERGLPTPLVLQRNSSTKDLTIFAAEAVALNRQLSQHDSD 365          

HSP 2 Score: 330.102 bits (845), Expect = 5.881e-105
Identity = 164/329 (49.85%), Postives = 216/329 (65.65%), Query Frame = 0

Query:   17 RLVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            R +ES +   F D+Y LT ++LGEG+Y +V+ C+++    +YAVKI+ K+    SR +V +E+E  Y CQG  NI++LIEF E+   F+L+FEK  GG +LS IQ+R HF E EA+ +++D+A AL FLH +GIAHRDLKPEN+LC   +   P+K+CDFDL SGV+ N     ITTP L +P GSAEYMAPEVV  F     F       YDK+CD+WSLG+I YI+L GY PF  G C  DCGW++GE C  C   LF+SI  G   F E  WS IS  A+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   23 RAMESFT-GKFADLYRLTDELLGEGAYAKVQGCVSLQNGKDYAVKIVEKKAG-HSRSRVFREVETLYQCQGNKNILELIEFCEDDARFYLVFEKLRGGSILSHIQKRKHFNEREASKVVKDIASALDFLHTKGIAHRDLKPENILCEFKDKVSPVKICDFDLGSGVKLNSACTPITTPELTTPCGSAEYMAPEVVEVFTEEATF-------YDKRCDLWSLGVILYIMLSGYPPF-VGNCGADCGWDRGEMCRVCQNKLFESIQEGKYEFPEKDWSHISNSAKDLISKLLVRDAKERLSAAQVLQHPWLQGDAPERGLPTPLVLQRNSS 341          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|82237505|sp|Q6P431.1|MKNK2_XENLA (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 2; AltName: Full=MAP kinase signal-integrating kinase 2; Short=MAPK signal-integrating kinase 2; Short=Mnk2)

HSP 1 Score: 326.635 bits (836), Expect = 7.655e-103
Identity = 165/319 (51.72%), Postives = 221/319 (69.28%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            F D+Y L  ++LGEG+Y +V++CIN+ T+ EYAVKII KR    SR +V +E+E+ Y CQG  N+++LIEFFEE + F+L+FEK  GG +L+ I RR HF E EA+ ++RD+A+AL +LH++GIAHRDLKPEN+LC S +   P+K+CDFDL SG++ N     I+TP L +P GSAEYMAPEVV AFN       ++   YDK+CD+WSLG+I YI+L GY PF  G C  DCGW++GE C  C   LF SI  G   F E  W+ ISY A+DLIS+LL+RDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   78 FDDMYQLQQEILGEGAYAKVQSCINLITNKEYAVKIIEKRPG-HSRSRVFREVEMLYQCQGHSNVLELIEFFEEEDKFYLVFEKMCGGSILNHIHRRRHFNEREASFVVRDIAEALNYLHNKGIAHRDLKPENILCESPHQVSPVKICDFDLGSGIKLNSDCSPISTPELLTPCGSAEYMAPEVVEAFN-------EEASIYDKRCDLWSLGVILYIMLSGYPPF-VGHCGSDCGWDRGEACPACQNMLFVSIQEGKYEFPEKDWAHISYGAKDLISKLLLRDAKKRLSAAQVLQHPWVQGNAPYNTLPTPIILQRNSS 387          

HSP 2 Score: 313.923 bits (803), Expect = 5.559e-98
Identity = 158/323 (48.92%), Postives = 216/323 (66.87%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNAS-----TKISTPKLQTPVGSLEYMAPEVSP---ERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNK 655
            YQL +E LG+G+ ++V + IN    +  AVK++ K  G  SRS + RE+E  + C+GH+N+L+L+EF+EE   FYLVFEKM GG +L+HI  R  F E+EA+ ++RD+A AL +LH KGIAHRDLKPEN+LC  P  + PVK+CDFDL S        + ISTP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++I+LSG PPF G CG +CGWD GEAC  CQ  L  +IQEG     +  WA+IS  AKDL+  LL ++  +R+    +L HPW+      + L TP +L+R++S + L+++A    A+N+
Sbjct:   82 YQLQQEILGEGAYAKVQSCINLITNKEYAVKIIEKRPG-HSRSRVFREVEMLYQCQGHSNVLELIEFFEEEDKFYLVFEKMCGGSILNHIHRRRHFNEREASFVVRDIAEALNYLHNKGIAHRDLKPENILCESPHQVSPVKICDFDLGSGIKLNSDCSPISTPELLTPCGSAEYMAPEVVEAFNEEASIYDKRCDLWSLGVILYIMLSGYPPFVGHCGSDCGWDRGEACPACQNMLFVSIQEGKYEFPEKDWAHISYGAKDLISKLLLRDAKKRLSAAQVLQHPWVQGNAPYNTLPTPIILQRNSSAKDLTSFAAEAIAMNR 403          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|30316115|sp|Q9BUB5.1|MKNK1_HUMAN (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 1; AltName: Full=MAP kinase signal-integrating kinase 1; Short=MAPK signal-integrating kinase 1; Short=Mnk1)

HSP 1 Score: 325.094 bits (832), Expect = 2.500e-102
Identity = 163/367 (44.41%), Postives = 227/367 (61.85%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKK-----------------------------------------GIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFS 658
            Y+LT E LG+G+ ++V  A++  NG+  AVK++ K  G  SRS + RE+ET + C+G+ NIL+L+EF+E+ T FYLVFEK++GG +L HI++++ F E+EA+R++RD+A AL FLH K                                         GIAHRDLKPEN+LC  P+ + PVK+CDFDL S     N+ T I+TP+L TP GS EYMAPEV     ++   Y+K+CDLWSLGV+++I+LSG PPF G CG +CGWD GE C  CQ KL  +IQEG        WA+IS  AKDL+  LL ++  +R+    +L HPW+  +     L TP+VL+R++ST  L+ +A    ALN+  S
Sbjct:   48 YKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLS 413          

HSP 2 Score: 310.842 bits (795), Expect = 8.158e-97
Identity = 160/360 (44.44%), Postives = 213/360 (59.17%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHR-----------------------------------------GIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            F+D+Y LT ++LGEG+Y +V+  +++    EYAVKII K+    SR +V +E+E  Y CQG  NI++LIEFFE+   F+L+FEK  GG +L+ IQ++ HF E EA+ ++RD+A AL FLH +                                         GIAHRDLKPEN+LC S     P+K+CDFDL SG++ N     ITTP L +P GSAEYMAPEVV  F     F       YDK+CD+WSLG++ YI+L GY PF  G C  DCGW++GE C  C   LF+SI  G   F +  W+ IS EA+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   44 FEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAG-HSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATF-------YDKRCDLWSLGVVLYIMLSGYPPFV-GHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSS 394          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|90102034|sp|Q8CDB0.3|MKNK2_MOUSE (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 2; AltName: Full=MAP kinase signal-integrating kinase 2; Short=MAPK signal-integrating kinase 2; Short=Mnk2)

HSP 1 Score: 324.709 bits (831), Expect = 3.399e-102
Identity = 165/318 (51.89%), Postives = 220/318 (69.18%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQT 339
            F+D+Y L  DVLGEG++ RV+TC+N+ T+ EYAVKII K+     R +V +E+E+ Y CQG  N+++LIEFFEE + F+L+FEK  GG +LS I RR HF E+EA+ +++D+A AL FLH++GIAHRDLKPEN+LC   N   P+K+CDFDL SG++ N     I+TP L +P GSAEYMAPEVV AF+       ++   YDK+CD+WSLG+I YILL GY PF  G C  DCGW++GE C  C   LF+SI  G   F +  WS IS+ A+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +
Sbjct:   79 FEDVYQLQEDVLGEGAHARVQTCVNLITNQEYAVKIIEKQ-LGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHRRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFS-------EEASIYDKRCDLWSLGVILYILLSGYPPF-VGHCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWSHISFAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPLVLQRNS 387          

HSP 2 Score: 309.686 bits (792), Expect = 1.593e-96
Identity = 160/336 (47.62%), Postives = 216/336 (64.29%), Query Frame = 0

Query:  336 RRQTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNAS-----TKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESE 663
            R +  YQL E+ LG+G+ ++V T +N    Q  AVK++ K  G   RS + RE+E  + C+GH N+L+L+EF+EE   FYLVFEKM GG +L HI  R  F E EA+ +++D+A AL FLH KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S        + ISTP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++ILLSG PPF G CG +CGWD GEAC  CQ  L  +IQEG        W++IS  AKDL+  LL ++  +R+    +L HPW+      + L TP VL+R++  + L+++A    A+N+  +  E +
Sbjct:   78 RFEDVYQLQEDVLGEGAHARVQTCVNLITNQEYAVKIIEKQLGHI-RSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHRRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGHCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWSHISFAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPLVLQRNSCAKDLTSFAAEAIAMNRQLAQCEED 412          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|81910241|sp|Q5U2N4.1|MKNK2_RAT (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 2; AltName: Full=MAP kinase signal-integrating kinase 2; Short=MAPK signal-integrating kinase 2; Short=Mnk2)

HSP 1 Score: 324.324 bits (830), Expect = 4.827e-102
Identity = 165/318 (51.89%), Postives = 220/318 (69.18%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQT 339
            F+D+Y L  DVLGEG++ RV+TC+N+ T+ EYAVKII K+     R +V +E+E+ Y CQG  N+++LIEFFEE + F+L+FEK  GG +LS I RR HF E+EA+ +++D+A AL FLH++GIAHRDLKPEN+LC   N   P+K+CDFDL SG++ N     I+TP L +P GSAEYMAPEVV AF+       ++   YDK+CD+WSLG+I YILL GY PF  G C  DCGW++GE C  C   LF+SI  G   F +  WS IS+ A+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +
Sbjct:   79 FEDVYQLQEDVLGEGAHARVQTCVNLITNQEYAVKIIEKQ-LGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHRRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFS-------EEASIYDKRCDLWSLGVILYILLSGYPPF-VGHCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWSHISFAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPLVLQRNS 387          

HSP 2 Score: 309.301 bits (791), Expect = 2.260e-96
Identity = 160/336 (47.62%), Postives = 216/336 (64.29%), Query Frame = 0

Query:  336 RRQTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNAS-----TKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESE 663
            R +  YQL E+ LG+G+ ++V T +N    Q  AVK++ K  G   RS + RE+E  + C+GH N+L+L+EF+EE   FYLVFEKM GG +L HI  R  F E EA+ +++D+A AL FLH KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S        + ISTP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++ILLSG PPF G CG +CGWD GEAC  CQ  L  +IQEG        W++IS  AKDL+  LL ++  +R+    +L HPW+      + L TP VL+R++  + L+++A    A+N+  +  E +
Sbjct:   78 RFEDVYQLQEDVLGEGAHARVQTCVNLITNQEYAVKIIEKQLGHI-RSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHRRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGHCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWSHISFAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPLVLQRNSCAKDLTSFAAEAIAMNRQLAQCEED 412          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|82234411|sp|Q66I46.1|MKNK2_XENTR (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 2; AltName: Full=MAP kinase signal-integrating kinase 2; Short=MAPK signal-integrating kinase 2; Short=Mnk2)

HSP 1 Score: 324.324 bits (830), Expect = 5.540e-102
Identity = 164/319 (51.41%), Postives = 221/319 (69.28%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            F+D+Y L  ++LGEG+Y +V++CIN+ T+ EYAVKII KR    SR +V +E+E+ Y CQG  N+++LIEFFEE + F+L+FEK  GG +L+ I RR HF E EA+ ++RD+A+AL +LH++GIAHRDLKPEN+LC S +   P+K+CDFDL SG++ N     I+TP L +P GSAEYMAPEVV AFN       ++   YDK+CD+WSLG+I YI+L GY PF  G C  DCGW++GE C  C   LF SI  G   F E  W+ IS  A+DLIS+LL+RDA  RL    +L+HP++        L TP +L+R +S
Sbjct:   78 FEDMYQLQQEILGEGAYAKVQSCINLITNKEYAVKIIEKRPG-HSRSRVFREVEMLYQCQGHSNVLELIEFFEEEDKFYLVFEKMCGGSILNHIHRRRHFNEREASFVVRDIAEALNYLHNKGIAHRDLKPENILCESPHQVSPVKICDFDLGSGIKLNSDCSPISTPELLTPCGSAEYMAPEVVEAFN-------EEASIYDKRCDLWSLGVILYIMLSGYPPF-VGHCGSDCGWDRGEACPACQNMLFVSIQEGKYEFPEKDWAHISSGAKDLISKLLLRDAKKRLSAAQVLQHPWVQGNAPDNTLPTPIILQRNSS 387          

HSP 2 Score: 315.079 bits (806), Expect = 1.495e-98
Identity = 159/328 (48.48%), Postives = 218/328 (66.46%), Query Frame = 0

Query:  336 RRQTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNAS-----TKISTPKLQTPVGSLEYMAPEVSP---ERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNK 655
            R +  YQL +E LG+G+ ++V + IN    +  AVK++ K  G  SRS + RE+E  + C+GH+N+L+L+EF+EE   FYLVFEKM GG +L+HI  R  F E+EA+ ++RD+A AL +LH KGIAHRDLKPEN+LC  P  + PVK+CDFDL S        + ISTP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++I+LSG PPF G CG +CGWD GEAC  CQ  L  +IQEG     +  WA+IS  AKDL+  LL ++  +R+    +L HPW+      + L TP +L+R++S + L+++A    A+N+
Sbjct:   77 RFEDMYQLQQEILGEGAYAKVQSCINLITNKEYAVKIIEKRPG-HSRSRVFREVEMLYQCQGHSNVLELIEFFEEEDKFYLVFEKMCGGSILNHIHRRRHFNEREASFVVRDIAEALNYLHNKGIAHRDLKPENILCESPHQVSPVKICDFDLGSGIKLNSDCSPISTPELLTPCGSAEYMAPEVVEAFNEEASIYDKRCDLWSLGVILYIMLSGYPPFVGHCGSDCGWDRGEACPACQNMLFVSIQEGKYEFPEKDWAHISSGAKDLISKLLLRDAKKRLSAAQVLQHPWVQGNAPDNTLPTPIILQRNSSAKDLTSFAAEAIAMNR 403          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. SwissProt
Match: gi|90102033|sp|Q9HBH9.3|MKNK2_HUMAN (RecName: Full=MAP kinase-interacting serine/threonine-protein kinase 2; AltName: Full=MAP kinase signal-integrating kinase 2; Short=MAPK signal-integrating kinase 2; Short=Mnk2)

HSP 1 Score: 322.398 bits (825), Expect = 2.943e-101
Identity = 164/318 (51.57%), Postives = 219/318 (68.87%), Query Frame = 0

Query:   27 FKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN-----ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQT 339
            F+D+Y L  DVLGEG++ RV+TCIN+ T  EYAVKII K+     R +V +E+E+ Y CQG  N+++LIEFFEE + F+L+FEK  GG +LS I +R HF E+EA+ +++D+A AL FLH++GIAHRDLKPEN+LC   N   P+K+CDFDL SG++ N     I+TP L +P GSAEYMAPEVV AF+       ++   YDK+CD+WSLG+I YILL GY PF  G+C  DCGW++GE C  C   LF+SI  G   F +  W+ IS  A+DLIS+LLVRDA  RL    +L+HP++        L TP +L+R +
Sbjct:   79 FEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHI-RSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFS-------EEASIYDKRCDLWSLGVILYILLSGYPPF-VGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNS 387          

HSP 2 Score: 313.923 bits (803), Expect = 4.710e-98
Identity = 168/365 (46.03%), Postives = 229/365 (62.74%), Query Frame = 0

Query:  336 RRQTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNAS-----TKISTPKLQTPVGSLEYMAPEVS---PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAFSFSESENHLVRDGLSPSGSAGLQRVAQPIFTRSSS 692
            R +  YQL E+ LG+G+ ++V T IN    Q  AVK++ K  G   RS + RE+E  + C+GH N+L+L+EF+EE   FYLVFEKM GG +L HI +R  F E EA+ +++D+A AL FLH KGIAHRDLKPEN+LC  P+ + PVK+CDFDL S        + ISTP+L TP GS EYMAPEV     E    Y+K+CDLWSLGVI++ILLSG PPF G CG +CGWD GEAC  CQ  L  +IQEG        WA+IS  AKDL+  LL ++  +R+    +L HPW+      + L TP VL+R++  + L+++A    A+N+  +  + +       L+   +AG     QP+  R++S
Sbjct:   78 RFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHI-RSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDLTSFAAEAIAMNRQLAQHDED-------LAEEEAAGQ---GQPVLVRATS 431          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1360798778|gb|AVN99021.1| (map kinase-interacting serine/threonine, partial [Procambarus clarkii])

HSP 1 Score: 382.489 bits (981), Expect = 1.679e-122
Identity = 184/328 (56.10%), Postives = 237/328 (72.26%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN------ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTSYQ 342
            +L  + F D+Y LTG+VLG+G+Y  V+TC+N++TD EYAVKII K     SR +V KE+E+++ CQG  NIIQL+EFFEE + F+L+FEK YGG LLS IQRR HFTE EA+ +IRDLA AL FLH +GIAHRDLKPEN+LC+  +   P+K+CDFDL SG++ N      + TP L +PVGSAE+MAPEVV       GF  DD   YDK+CD+WSLG++ YILLCGY PF  G C +DCGWE+GE C +C   LF++I  G+  F +  WSSIS E +DLI +LLV+DAS RL  + +L HP+I NGG S  LETP+++R+  S Q
Sbjct:   38 TLVASVFSDLYKLTGEVLGQGAYASVQTCVNIYTDIEYAVKIIEKVP-SHSRTRVFKEVEMFHHCQGHKNIIQLVEFFEEEDKFYLVFEKIYGGPLLSHIQRRTHFTEAEASMVIRDLASALAFLHSKGIAHRDLKPENILCVYPDQLCPVKICDFDLGSGIKFNSNLSSPVVTPQLLTPVGSAEFMAPEVVE------GFISDDAGPYDKRCDLWSLGVVTYILLCGYPPF-CGNCGEDCGWERGEACPQCQDLLFNNIQEGSYEFPKPEWSSISEEGKDLIRKLLVKDASQRLSAEMVLSHPWIRNGGPSTRLETPNVIRKNHSAQ 357          

HSP 2 Score: 344.739 bits (883), Expect = 6.771e-108
Identity = 174/338 (51.48%), Postives = 226/338 (66.86%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLA------SNASTKISTPKLQTPVGSLEYMAPEVS----PERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKA----FSFSESEN 664
            Y+LT E LGQG+ + V T +N       AVK++ K     SR+ + +E+E FH C+GH NI+QL+EF+EE   FYLVFEK+ GG LL HI+ R  FTE EA+ +IRDLA AL FLH KGIAHRDLKPEN+LC+ PD + PVK+CDFDL       SN S+ + TP+L TPVGS E+MAPEV      +    Y+K+CDLWSLGV+ +ILL G PPF G+CG++CGW+ GEAC  CQ  L   IQEGS    KP W++IS+  KDL+  LL K+  +R+    +L HPWI + G S RL TP V+R++ S Q LS +A S  ALN+     FS ++ E+
Sbjct:   48 YKLTGEVLGQGAYASVQTCVNIYTDIEYAVKIIEK-VPSHSRTRVFKEVEMFHHCQGHKNIIQLVEFFEEEDKFYLVFEKIYGGPLLSHIQRRTHFTEAEASMVIRDLASALAFLHSKGIAHRDLKPENILCVYPDQLCPVKICDFDLGSGIKFNSNLSSPVVTPQLLTPVGSAEFMAPEVVEGFISDDAGPYDKRCDLWSLGVVTYILLCGYPPFCGNCGEDCGWERGEACPQCQDLLFNNIQEGSYEFPKPEWSSISEEGKDLIRKLLVKDASQRLSAEMVLSHPWIRNGGPSTRLETPNVIRKNHSAQQLSLFAESCMALNRVLLQHFSMNQDED 384          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1316137683|ref|XP_023241744.1| (MAP kinase-interacting serine/threonine-protein kinase 1-like isoform X2 [Centruroides sculpturatus])

HSP 1 Score: 382.104 bits (980), Expect = 1.624e-121
Identity = 186/324 (57.41%), Postives = 234/324 (72.22%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPL----LQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            SL+C+ F D+Y LT ++LGEG+Y  V TCIN++T+ EYAVKII K     SR +V +E+E ++ CQG  NIIQLIEFFEE + F+L+FEK  GG LL+ IQ+RVHFTE EA++IIRD+A AL+FLHH+GIAHRDLKPEN+LC S N   P+K+CDFDL SG+  N TTP+    LQ+PVGSAE+MAPEVV AF         +  TYDK+CD+WSLG+I YILLCGY PF  G+C  DCGWE+GE C  C   LF  I +G   F E  W+ IS EA+DLI  LLV+DAS R   + +L HP+I +GG +  LETPS++RR  S
Sbjct:   89 SLACSDFSDLYRLTEEILGEGAYASVRTCINVYTEKEYAVKIIEKEPG-HSRSRVFREVETFHHCQGHQNIIQLIEFFEEQDRFYLVFEKVNGGPLLAHIQKRVHFTENEASTIIRDIATALKFLHHKGIAHRDLKPENILCFSENQVCPVKICDFDLGSGIVFNSTTPVSTPELQTPVGSAEFMAPEVVEAFI-------GEATTYDKRCDLWSLGVIMYILLCGYPPF-YGRCGSDCGWERGEFCQSCQDMLFTCIQDGAYYFPEREWAYISEEAKDLIRHLLVKDASQRYTAEMVLNHPWISHGGPTTRLETPSIIRRNNS 403          

HSP 2 Score: 345.51 bits (885), Expect = 2.539e-107
Identity = 171/322 (53.11%), Postives = 225/322 (69.88%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS----NASTKISTPKLQTPVGSLEYMAPEVSPE---RRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNK 655
            Y+LTEE LG+G+ + V T IN    +  AVK++ K  G  SRS + RE+ETFH C+GH NI+QL+EF+EE   FYLVFEK+ GG LL HI++R  FTE EA+ IIRD+A AL+FLH KGIAHRDLKPEN+LC   + + PVK+CDFDL S    N++T +STP+LQTPVGS E+MAPEV         +Y+K+CDLWSLGVIM+ILL G PPF G CG +CGW+ GE C +CQ  L T IQ+G+    +  WA IS+ AKDL+ +LL K+  +R     +L HPWI+  G + RL TP ++RR+ S + L+ +A S  A+ +
Sbjct:   99 YRLTEEILGEGAYASVRTCINVYTEKEYAVKIIEKEPG-HSRSRVFREVETFHHCQGHQNIIQLIEFFEEQDRFYLVFEKVNGGPLLAHIQKRVHFTENEASTIIRDIATALKFLHHKGIAHRDLKPENILCFSENQVCPVKICDFDLGSGIVFNSTTPVSTPELQTPVGSAEFMAPEVVEAFIGEATTYDKRCDLWSLGVIMYILLCGYPPFYGRCGSDCGWERGEFCQSCQDMLFTCIQDGAYYFPEREWAYISEEAKDLIRHLLVKDASQRYTAEMVLNHPWISHGGPTTRLETPSIIRRNNSAKDLAAFAESANAMKR 419          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1316137681|ref|XP_023241736.1| (MAP kinase-interacting serine/threonine-protein kinase 1-like isoform X1 [Centruroides sculpturatus])

HSP 1 Score: 382.104 bits (980), Expect = 2.048e-121
Identity = 186/324 (57.41%), Postives = 234/324 (72.22%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQNITTPL----LQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            SL+C+ F D+Y LT ++LGEG+Y  V TCIN++T+ EYAVKII K     SR +V +E+E ++ CQG  NIIQLIEFFEE + F+L+FEK  GG LL+ IQ+RVHFTE EA++IIRD+A AL+FLHH+GIAHRDLKPEN+LC S N   P+K+CDFDL SG+  N TTP+    LQ+PVGSAE+MAPEVV AF         +  TYDK+CD+WSLG+I YILLCGY PF  G+C  DCGWE+GE C  C   LF  I +G   F E  W+ IS EA+DLI  LLV+DAS R   + +L HP+I +GG +  LETPS++RR  S
Sbjct:   96 SLACSDFSDLYRLTEEILGEGAYASVRTCINVYTEKEYAVKIIEKEPG-HSRSRVFREVETFHHCQGHQNIIQLIEFFEEQDRFYLVFEKVNGGPLLAHIQKRVHFTENEASTIIRDIATALKFLHHKGIAHRDLKPENILCFSENQVCPVKICDFDLGSGIVFNSTTPVSTPELQTPVGSAEFMAPEVVEAFI-------GEATTYDKRCDLWSLGVIMYILLCGYPPF-YGRCGSDCGWERGEFCQSCQDMLFTCIQDGAYYFPEREWAYISEEAKDLIRHLLVKDASQRYTAEMVLNHPWISHGGPTTRLETPSIIRRNNS 410          

HSP 2 Score: 345.51 bits (885), Expect = 2.915e-107
Identity = 171/322 (53.11%), Postives = 225/322 (69.88%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS----NASTKISTPKLQTPVGSLEYMAPEVSPE---RRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNK 655
            Y+LTEE LG+G+ + V T IN    +  AVK++ K  G  SRS + RE+ETFH C+GH NI+QL+EF+EE   FYLVFEK+ GG LL HI++R  FTE EA+ IIRD+A AL+FLH KGIAHRDLKPEN+LC   + + PVK+CDFDL S    N++T +STP+LQTPVGS E+MAPEV         +Y+K+CDLWSLGVIM+ILL G PPF G CG +CGW+ GE C +CQ  L T IQ+G+    +  WA IS+ AKDL+ +LL K+  +R     +L HPWI+  G + RL TP ++RR+ S + L+ +A S  A+ +
Sbjct:  106 YRLTEEILGEGAYASVRTCINVYTEKEYAVKIIEKEPG-HSRSRVFREVETFHHCQGHQNIIQLIEFFEEQDRFYLVFEKVNGGPLLAHIQKRVHFTENEASTIIRDIATALKFLHHKGIAHRDLKPENILCFSENQVCPVKICDFDLGSGIVFNSTTPVSTPELQTPVGSAEFMAPEVVEAFIGEATTYDKRCDLWSLGVIMYILLCGYPPFYGRCGSDCGWERGEFCQSCQDMLFTCIQDGAYYFPEREWAYISEEAKDLIRHLLVKDASQRYTAEMVLNHPWISHGGPTTRLETPSIIRRNNSAKDLAAFAESANAMKR 426          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1022763203|gb|KZS08840.1| (MAP kinase-interacting serine/threonine-protein kinase 1 [Daphnia magna])

HSP 1 Score: 375.555 bits (963), Expect = 4.894e-119
Identity = 184/328 (56.10%), Postives = 234/328 (71.34%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN------ITTPLLQSPVGSAEYMAPEVVHAF--NTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            SL  +TFK++Y LTG+ LG+G+Y  V+TC+N++TD EYAVKII K     SR +V KE+E ++ CQG PNIIQLIEFFEE + F+L+FEK  GG LLS IQRR+HFTE EA+ ++RDLA  LQFLH +GIAHRDLKPEN+LC+  +   P+K+CDFDL SG++ N      I+TP L +PVGSAE+MAPEVV AF  +  NG        YDK+CD+WSLGI+ YILLCGY PF  G C  DCGWE+GE C  C   LF SI  G   F E  W+ IS+EA++LI  LLV++A  RL  +++LEHP++ NGG   PL TP ++RR  S
Sbjct:   32 SLFASTFKELYKLTGEFLGQGAYASVQTCVNIWTDVEYAVKIIEKVP-GHSRDRVFKEVETFHHCQGHPNIIQLIEFFEEEDRFYLVFEKILGGPLLSHIQRRIHFTEHEASLVLRDLAAGLQFLHKKGIAHRDLKPENILCVYPDRLTPVKICDFDLGSGIKFNSNLNSPISTPELLTPVGSAEFMAPEVVDAFVGHVING--------YDKRCDLWSLGIVMYILLCGYPPF-YGHCGADCGWERGEACQSCQGLLFTSIQEGRYEFPEREWAHISHEAKELIRGLLVKEAPRRLSAQSVLEHPWVKNGGPRTPLITPQVIRRNNS 349          

HSP 2 Score: 338.576 bits (867), Expect = 7.641e-105
Identity = 170/326 (52.15%), Postives = 221/326 (67.79%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLA------SNASTKISTPKLQTPVGSLEYMAPEVSP----ERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKA 656
            Y+LT E LGQG+ + V T +N       AVK++ K  G  SR  + +E+ETFH C+GH NI+QL+EF+EE   FYLVFEK+ GG LL HI+ R  FTE EA+ ++RDLA  LQFLHKKGIAHRDLKPEN+LC+ PD + PVK+CDFDL       SN ++ ISTP+L TPVGS E+MAPEV           Y+K+CDLWSLG++M+ILL G PPF G CG +CGW+ GEAC +CQ  L T+IQEG     +  WA+IS  AK+L+  LL K   RR+  +S+L HPW+ + G    L TP+V+RR+ S + LS +A S  A+ + 
Sbjct:   42 YKLTGEFLGQGAYASVQTCVNIWTDVEYAVKIIEKVPG-HSRDRVFKEVETFHHCQGHPNIIQLIEFFEEEDRFYLVFEKILGGPLLSHIQRRIHFTEHEASLVLRDLAAGLQFLHKKGIAHRDLKPENILCVYPDRLTPVKICDFDLGSGIKFNSNLNSPISTPELLTPVGSAEFMAPEVVDAFVGHVINGYDKRCDLWSLGIVMYILLCGYPPFYGHCGADCGWERGEACQSCQGLLFTSIQEGRYEFPEREWAHISHEAKELIRGLLVKEAPRRLSAQSVLEHPWVKNGGPRTPLITPQVIRRNNSARELSVFAESAMAVKRV 366          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|321464825|gb|EFX75830.1| (hypothetical protein DAPPUDRAFT_306600 [Daphnia pulex])

HSP 1 Score: 371.703 bits (953), Expect = 1.875e-117
Identity = 184/328 (56.10%), Postives = 231/328 (70.43%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN------ITTPLLQSPVGSAEYMAPEVVHAF--NTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            SL  +TF ++Y LTG+ LG+G+Y  V+TC+N++TD EYAVKII K     SR +V KE+E ++ CQG PNIIQLIEFFEE + F+L+FEK  GG LLS IQRRVHFTE EA+ ++RDLA  LQFLH +GIAHRDLKPEN+LC+  +   P+K+CDFDL SG++ N      I+TP L +PVGSAE+MAPEVV AF     NG        YDK+CD+WSLGI+ YILLCGY PF  G C  DCGWE+GE C  C   LF SI  G   F +  W+ IS EA+DLI  LLV++A  RL  +++LEHP++ NGG   PL TP ++RR  S
Sbjct:   32 SLFASTFNELYRLTGEFLGQGAYASVQTCVNIWTDVEYAVKIIEKVP-GHSRDRVFKEVETFHHCQGHPNIIQLIEFFEEEDRFYLVFEKILGGPLLSHIQRRVHFTEHEASLVLRDLAAGLQFLHKKGIAHRDLKPENILCVYPDRLTPVKICDFDLGSGIKFNSNLNSPISTPELLTPVGSAEFMAPEVVDAFVGQLING--------YDKRCDLWSLGIVMYILLCGYPPF-YGHCGADCGWERGEACQSCQGLLFTSIQEGRYEFPDREWAHISQEAKDLIRGLLVKEAPRRLSAQSVLEHPWVKNGGPRTPLITPQVIRRNNS 349          

HSP 2 Score: 342.428 bits (877), Expect = 4.184e-106
Identity = 172/326 (52.76%), Postives = 222/326 (68.10%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLA------SNASTKISTPKLQTPVGSLEYMAPEVSP----ERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKA 656
            Y+LT E LGQG+ + V T +N       AVK++ K  G  SR  + +E+ETFH C+GH NI+QL+EF+EE   FYLVFEK+ GG LL HI+ R  FTE EA+ ++RDLA  LQFLHKKGIAHRDLKPEN+LC+ PD + PVK+CDFDL       SN ++ ISTP+L TPVGS E+MAPEV      +    Y+K+CDLWSLG++M+ILL G PPF G CG +CGW+ GEAC +CQ  L T+IQEG        WA+ISQ AKDL+  LL K   RR+  +S+L HPW+ + G    L TP+V+RR+ S + LS +A S  A+ + 
Sbjct:   42 YRLTGEFLGQGAYASVQTCVNIWTDVEYAVKIIEKVPG-HSRDRVFKEVETFHHCQGHPNIIQLIEFFEEEDRFYLVFEKILGGPLLSHIQRRVHFTEHEASLVLRDLAAGLQFLHKKGIAHRDLKPENILCVYPDRLTPVKICDFDLGSGIKFNSNLNSPISTPELLTPVGSAEFMAPEVVDAFVGQLINGYDKRCDLWSLGIVMYILLCGYPPFYGHCGADCGWERGEACQSCQGLLFTSIQEGRYEFPDREWAHISQEAKDLIRGLLVKEAPRRLSAQSVLEHPWVKNGGPRTPLITPQVIRRNNSARELSVFAESAMAVKRV 366          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|926635214|ref|XP_013783488.1| (MAP kinase-interacting serine/threonine-protein kinase 1-like [Limulus polyphemus])

HSP 1 Score: 370.163 bits (949), Expect = 1.121e-116
Identity = 181/324 (55.86%), Postives = 230/324 (70.99%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGV----QQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            SL+ +TF+D+Y LTG++LGEG+Y  V TCIN++T  EYAVKII K     SR +V +EIE ++ CQGQ NIIQLIEFFEE + F+L+FEK  GG LL+ I++RVHFTE E + IIRD+A+AL+FLH +GIAHRDLKPEN+LC S +   P+K+CDFDL SG+       ++TP L +PVGSAE+MAPEVV AF         +  TYDK+CD+WSLG+I YILLCGY PF  G+C  DCGWE GE C  C   LF  I  G   F E  WSSIS EA+DLI  LLV+DAS R   + +L HP++ +GG +  L TP++LR+  S
Sbjct:   99 SLAASTFEDLYRLTGEILGEGAYASVRTCINLYTGNEYAVKIIEKEP-SHSRARVFREIETFHHCQGQKNIIQLIEFFEEEDRFYLVFEKICGGPLLAHIEKRVHFTENEVSIIIRDIAEALKFLHKKGIAHRDLKPENILCFSEDQVYPVKICDFDLSSGIIFGSTTPVSTPELLTPVGSAEFMAPEVVEAFT-------GEATTYDKRCDLWSLGVIMYILLCGYPPF-YGRCGSDCGWEHGEFCEACQDMLFTCIQEGMYDFCEREWSSISEEAKDLIRHLLVKDASQRYTAETVLSHPWVKHGGPTIFLNTPNILRKNKS 413          

HSP 2 Score: 332.798 bits (852), Expect = 2.451e-102
Identity = 165/322 (51.24%), Postives = 221/322 (68.63%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS----NASTKISTPKLQTPVGSLEYMAPEVSPE---RRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNK 655
            Y+LT E LG+G+ + V T IN   G   AVK++ K     SR+ + REIETFH C+G  NI+QL+EF+EE   FYLVFEK+ GG LL HI++R  FTE E + IIRD+A AL+FLHKKGIAHRDLKPEN+LC   D ++PVK+CDFDL+S     ++T +STP+L TPVGS E+MAPEV         +Y+K+CDLWSLGVIM+ILL G PPF G CG +CGW++GE C  CQ  L T IQEG  +  +  W++IS+ AKDL+ +LL K+  +R    ++L HPW+   G +  L TP +LR++ S + L+ +  S  A+ +
Sbjct:  109 YRLTGEILGEGAYASVRTCINLYTGNEYAVKIIEKEPS-HSRARVFREIETFHHCQGQKNIIQLIEFFEEEDRFYLVFEKICGGPLLAHIEKRVHFTENEVSIIIRDIAEALKFLHKKGIAHRDLKPENILCFSEDQVYPVKICDFDLSSGIIFGSTTPVSTPELLTPVGSAEFMAPEVVEAFTGEATTYDKRCDLWSLGVIMYILLCGYPPFYGRCGSDCGWEHGEFCEACQDMLFTCIQEGMYDFCEREWSSISEEAKDLIRHLLVKDASQRYTAETVLSHPWVKHGGPTIFLNTPNILRKNKSARDLAAFTESANAVKR 429          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|198438098|ref|XP_002131954.1| (PREDICTED: MAP kinase-interacting serine/threonine-protein kinase 1 [Ciona intestinalis])

HSP 1 Score: 363.614 bits (932), Expect = 7.541e-114
Identity = 180/322 (55.90%), Postives = 224/322 (69.57%), Query Frame = 0

Query:   24 CN-TFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSG----VQQNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTS 340
            CN TF+D+YY+ G++LGEG+Y  V TCIN +T  EYAVKII K     SR KV KE+EL + C G+PNI+QLIE+FE  E F+L+FEK YGG LL +I+R  HF E EAA ++RD+A AL FLH +GIAHRDLKP N+LC +A+   P+K+CDFDL SG    + + I TP L SPVGSAEYMAPEVV  F     F+E   L+YDK+CDIWSLG+I YI+L G+ PF  G C +DCGW+ GE C  C   LF SI +G   F E  WS IS +A+DLIS L+VRD S+R +   ILEHP+I    S  PL TP +L R+ S
Sbjct:  104 CNGTFEDLYYINGELLGEGAYATVWTCINKYTHKEYAVKIIEKHVAGHSRMKVFKEVELLHQCAGRPNILQLIEYFETNEKFYLVFEKMYGGTLLHRIERCEHFDECEAARVVRDIATALAFLHDKGIAHRDLKPANILCETADKVSPVKICDFDLASGCRSSMMEPIKTPELSSPVGSAEYMAPEVVDTFV----FEE--SLSYDKRCDIWSLGVITYIMLSGHPPF-VGACSEDCGWDVGEACQTCQDMLFASIKSGEYSFPEEQWSGISNDAKDLISHLIVRDRSMRYNAHQILEHPWIKGTASETPLSTPQLLSRRDS 418          

HSP 2 Score: 309.686 bits (792), Expect = 4.233e-93
Identity = 161/356 (45.22%), Postives = 218/356 (61.24%), Query Frame = 0

Query:  341 YQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLASNASTK----ISTPKLQTPVGSLEYMAPEVSP----ERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVL-RRHASTQGLSNYATSTFALNKAFSFSESENHLVRDGLS------PSGSAGLQR 681
            Y +  E LG+G+ + V T IN+   +  AVK++ K+    SR  + +E+E  H C G  NILQL+E++E    FYLVFEKM GG LL  I+  E F E EAAR++RD+A AL FLH KGIAHRDLKP N+LC   D + PVK+CDFDLAS   +     I TP+L +PVGS EYMAPEV      E   SY+K+CD+WSLGVI +I+LSG PPF G C ++CGWD GEAC TCQ  L  +I+ G  +  +  W+ IS  AKDL+ +L+ ++   R +   +L HPWI    +   L TP++L RR +ST  L+ +A    A  +  + +++    +  G+S      P  SA  QR
Sbjct:  112 YYINGELLGEGAYATVWTCINKYTHKEYAVKIIEKHVAGHSRMKVFKEVELLHQCAGRPNILQLIEYFETNEKFYLVFEKMYGGTLLHRIERCEHFDECEAARVVRDIATALAFLHDKGIAHRDLKPANILCETADKVSPVKICDFDLASGCRSSMMEPIKTPELSSPVGSAEYMAPEVVDTFVFEESLSYDKRCDIWSLGVITYIMLSGHPPFVGACSEDCGWDVGEACQTCQDMLFASIKSGEYSFPEEQWSGISNDAKDLISHLIVRDRSMRYNAHQILEHPWIKGTASETPLSTPQLLSRRDSSTHDLTQFAAHAVAYERKLACNDTST--ITQGISNVRLSTPGTSALAQR 465          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1101339155|ref|XP_018917801.1| (PREDICTED: MAP kinase-interacting serine/threonine-protein kinase 1-like isoform X2 [Bemisia tabaci])

HSP 1 Score: 360.147 bits (923), Expect = 9.869e-114
Identity = 181/341 (53.08%), Postives = 234/341 (68.62%), Query Frame = 0

Query:   10 VKRKKKKRLVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN------ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSA----PLETPSMLRRQTS 340
            VKR+KKK    SL  + F+D+Y LTG+VLGEG+Y  V+TC+N+ TD EYAVKII K     SR +V KE+E ++ C G  NIIQL+EFFE+ + F+L+FEK  GGQLLS+IQ R HF+EVEA+ II+DLA AL +LH +GIAHRDLKPEN+LC+  +   P+KLCDFDL SG++ N      + TP L +PVGSAE+MAPEVV+AF   +         YDK+CD+WSLG++ YILLCGY PF  G C  DCGWE+GE C +C   LF SI  G   F +  W+ IS +A+DLI +LLV++AS RL    +L H ++  G S      PL TP ++RR  S
Sbjct:   26 VKRRKKK-TATSLVTSCFQDLYRLTGEVLGEGAYASVQTCVNILTDMEYAVKIIEKTAE-HSRARVFKEVETFHHCAGHQNIIQLLEFFEDDDRFYLVFEKVEGGQLLSRIQERTHFSEVEASQIIKDLASALNYLHKKGIAHRDLKPENILCVDRHKLTPVKLCDFDLGSGIKLNAALCSPLATPQLLTPVGSAEFMAPEVVNAFTGESN-------AYDKRCDLWSLGVVMYILLCGYPPF-YGNCGMDCGWERGETCMECQELLFSSIQEGRYDFPDREWAYISEDAKDLIRKLLVKEASHRLSAAEVLNHRWVNPGPSQTHAGRPLVTPQVIRRNNS 356          

HSP 2 Score: 323.168 bits (827), Expect = 1.880e-99
Identity = 166/331 (50.15%), Postives = 221/331 (66.77%), Query Frame = 0

Query:  338 QTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS----NAS--TKISTPKLQTPVGSLEYMAPEVSPE---RRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLG----TPRVLRRHASTQGLSNYATSTFALNK 655
            Q  Y+LT E LG+G+ + V T +N       AVK++ K T   SR+ + +E+ETFH C GH NI+QLLEF+E+   FYLVFEK+EGGQLL  I+ER  F+E EA++II+DLA AL +LHKKGIAHRDLKPEN+LC+    + PVKLCDFDL S    NA+  + ++TP+L TPVGS E+MAPEV         +Y+K+CDLWSLGV+M+ILL G PPF G+CG +CGW+ GE C+ CQ  L ++IQEG  +     WA IS+ AKDL+  LL K    R+    +L H W+    +    G    TP+V+RR+ S + LS +A S  A+N+
Sbjct:   43 QDLYRLTGEVLGEGAYASVQTCVNILTDMEYAVKIIEK-TAEHSRARVFKEVETFHHCAGHQNIIQLLEFFEDDDRFYLVFEKVEGGQLLSRIQERTHFSEVEASQIIKDLASALNYLHKKGIAHRDLKPENILCVDRHKLTPVKLCDFDLGSGIKLNAALCSPLATPQLLTPVGSAEFMAPEVVNAFTGESNAYDKRCDLWSLGVVMYILLCGYPPFYGNCGMDCGWERGETCMECQELLFSSIQEGRYDFPDREWAYISEDAKDLIRKLLVKEASHRLSAAEVLNHRWVNPGPSQTHAGRPLVTPQVIRRNNSARELSVFAESAMAVNR 372          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1101339153|ref|XP_018917799.1| (PREDICTED: MAP kinase-interacting serine/threonine-protein kinase 1-like isoform X1 [Bemisia tabaci])

HSP 1 Score: 360.533 bits (924), Expect = 1.506e-113
Identity = 181/341 (53.08%), Postives = 234/341 (68.62%), Query Frame = 0

Query:   10 VKRKKKKRLVESLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQQN------ITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSA----PLETPSMLRRQTS 340
            VKR+KKK    SL  + F+D+Y LTG+VLGEG+Y  V+TC+N+ TD EYAVKII K     SR +V KE+E ++ C G  NIIQL+EFFE+ + F+L+FEK  GGQLLS+IQ R HF+EVEA+ II+DLA AL +LH +GIAHRDLKPEN+LC+  +   P+KLCDFDL SG++ N      + TP L +PVGSAE+MAPEVV+AF   +         YDK+CD+WSLG++ YILLCGY PF  G C  DCGWE+GE C +C   LF SI  G   F +  W+ IS +A+DLI +LLV++AS RL    +L H ++  G S      PL TP ++RR  S
Sbjct:   45 VKRRKKK-TATSLVTSCFQDLYRLTGEVLGEGAYASVQTCVNILTDMEYAVKIIEKTAE-HSRARVFKEVETFHHCAGHQNIIQLLEFFEDDDRFYLVFEKVEGGQLLSRIQERTHFSEVEASQIIKDLASALNYLHKKGIAHRDLKPENILCVDRHKLTPVKLCDFDLGSGIKLNAALCSPLATPQLLTPVGSAEFMAPEVVNAFTGESN-------AYDKRCDLWSLGVVMYILLCGYPPF-YGNCGMDCGWERGETCMECQELLFSSIQEGRYDFPDREWAYISEDAKDLIRKLLVKEASHRLSAAEVLNHRWVNPGPSQTHAGRPLVTPQVIRRNNS 375          

HSP 2 Score: 323.553 bits (828), Expect = 2.392e-99
Identity = 166/332 (50.00%), Postives = 221/332 (66.57%), Query Frame = 0

Query:  338 QTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS----NAS--TKISTPKLQTPVGSLEYMAPEVSPE---RRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLG----TPRVLRRHASTQGLSNYATSTFALNKA 656
            Q  Y+LT E LG+G+ + V T +N       AVK++ K T   SR+ + +E+ETFH C GH NI+QLLEF+E+   FYLVFEK+EGGQLL  I+ER  F+E EA++II+DLA AL +LHKKGIAHRDLKPEN+LC+    + PVKLCDFDL S    NA+  + ++TP+L TPVGS E+MAPEV         +Y+K+CDLWSLGV+M+ILL G PPF G+CG +CGW+ GE C+ CQ  L ++IQEG  +     WA IS+ AKDL+  LL K    R+    +L H W+    +    G    TP+V+RR+ S + LS +A S  A+N+ 
Sbjct:   62 QDLYRLTGEVLGEGAYASVQTCVNILTDMEYAVKIIEK-TAEHSRARVFKEVETFHHCAGHQNIIQLLEFFEDDDRFYLVFEKVEGGQLLSRIQERTHFSEVEASQIIKDLASALNYLHKKGIAHRDLKPENILCVDRHKLTPVKLCDFDLGSGIKLNAALCSPLATPQLLTPVGSAEFMAPEVVNAFTGESNAYDKRCDLWSLGVVMYILLCGYPPFYGNCGMDCGWERGETCMECQELLFSSIQEGRYDFPDREWAYISEDAKDLIRKLLVKEASHRLSAAEVLNHRWVNPGPSQTHAGRPLVTPQVIRRNNSARELSVFAESAMAVNRV 392          

BLAST of hypothetical protein DAPPUDRAFT_306600 vs. nr
Match: gi|1325272971|ref|XP_023321119.1| (MAP kinase-interacting serine/threonine-protein kinase 1-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 360.147 bits (923), Expect = 1.527e-113
Identity = 181/357 (50.70%), Postives = 242/357 (67.79%), Query Frame = 0

Query:   21 SLSCNTFKDIYYLTGDVLGEGSYGRVETCINMFTDTEYAVKIISKRNWCFSRGKVLKEIELYYLCQGQPNIIQLIEFFEEPEYFFLIFEKAYGGQLLSQIQRRVHFTEVEAASIIRDLAQALQFLHHRGIAHRDLKPENVLCLSANSPLPIKLCDFDLCSGVQ-----QNITTPLLQSPVGSAEYMAPEVVHAFNTTNGFDEDDELTYDKKCDIWSLGIIAYILLCGYLPFAAGKCDQDCGWEKGEECSKCTRSLFDSICNGTLVFHEHYWSSISYEAQDLISRLLVRDASLRLDTKAILEHPFIVNGGSSAPLETPSMLRRQTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKV 372
            SL+ NTF+++Y LT D+LG+G+Y  V TCIN++T+ EYAVKII K     SR +V KEI+ ++ CQG  NIIQL+E+FEE E F+L+FEK  GGQLL  IQRR  F+E EAA I+RD+A ALQFLH +GIAHRDLKPENVLC+  +   P+KLCDFDL SG++      + TTPLL +PVGSAE+MAPE+V AF   N  D    L YDK+CD+WSLG++ YILLCGY PF +G+C   CGW +GE C  C  +LF++I  G   FH+  WS +S EA+DLIS+LL++DA +RL    +L HP++    ++   E PS     T ++L          ++  +A+NR   Q  +V +
Sbjct:   46 SLASNTFQELYRLTDDILGQGAYASVRTCINIYTEMEYAVKIIDKVPG-HSRTRVFKEIDTFHHCQGHANIIQLVEYFEESERFYLVFEKVRGGQLLDHIQRRKCFSEREAAMIVRDVASALQFLHSKGIAHRDLKPENVLCVHPDRITPVKLCDFDLGSGIKFHSGGGSDTTPLLLTPVGSAEFMAPEIVEAFIEDNELD----LKYDKRCDLWSLGVMLYILLCGYPPF-SGQCGSACGWNQGESCDDCQANLFNNIQEGRFEFHKEEWSHVSAEAKDLISKLLIKDARMRLSAAKVLSHPWLETNSTT---ELPS----STMFRLNNNAKHLNEFAESASAVNRVMLQHCSVNL 389          

HSP 2 Score: 328.176 bits (840), Expect = 3.464e-101
Identity = 165/331 (49.85%), Postives = 220/331 (66.47%), Query Frame = 0

Query:  338 QTSYQLTEEQLGQGSSSQVVTAINRSNGQALAVKVVAKNTGMFSRSNMLREIETFHLCEGHANILQLLEFYEEATCFYLVFEKMEGGQLLDHIKERELFTEKEAARIIRDLARALQFLHKKGIAHRDLKPENVLCLRPDSIHPVKLCDFDLAS-----NASTKISTPKLQTPVGSLEYMAPEV------SPERRRSYNKKCDLWSLGVIMHILLSGSPPFSGDCGQECGWDNGEACLTCQTKLSTAIQEGSVNLDKPFWANISQPAKDLLLNLLEKNVDRRIDTRSLLIHPWITSEGTSHRLGTPRVLRRHASTQGLSNYATSTFALNKAF 657
            Q  Y+LT++ LGQG+ + V T IN       AVK++ K  G  SR+ + +EI+TFH C+GHANI+QL+E++EE+  FYLVFEK+ GGQLLDHI+ R+ F+E+EAA I+RD+A ALQFLH KGIAHRDLKPENVLC+ PD I PVKLCDFDL S     +     +TP L TPVGS E+MAPE+        E    Y+K+CDLWSLGV+++ILL G PPFSG CG  CGW+ GE+C  CQ  L   IQEG     K  W+++S  AKDL+  LL K+   R+    +L HPW+ +  T+  L +  + R + + + L+ +A S  A+N+  
Sbjct:   53 QELYRLTDDILGQGAYASVRTCINIYTEMEYAVKIIDKVPG-HSRTRVFKEIDTFHHCQGHANIIQLVEYFEESERFYLVFEKVRGGQLLDHIQRRKCFSEREAAMIVRDVASALQFLHSKGIAHRDLKPENVLCVHPDRITPVKLCDFDLGSGIKFHSGGGSDTTPLLLTPVGSAEFMAPEIVEAFIEDNELDLKYDKRCDLWSLGVMLYILLCGYPPFSGQCGSACGWNQGESCDDCQANLFNNIQEGRFEFHKEEWSHVSAEAKDLISKLLIKDARMRLSAAKVLSHPWLETNSTT-ELPSSTMFRLNNNAKHLNEFAESASAVNRVM 381