Rhabdovirus - No9 test

Submitted by Aina-Cathrine Ø... on 20 April, 2018 - 08:59

General

Experiment Description:

Test knock-Down of rhabdoviral RNAs

Experiment Batch ID:

Exp1

Start Date:

Thursday, January 1, 2015 - 14:11

Experiment Contact:

Aina-Cathrine Øvergård

Frank Nilsen

Publication:

RNAi-mediated treatment of two vertically transmitted rhabdovirus infecting the salmon louse (Lepeophtheirus salmonis)

Sample

Developmental Stage CV:

Nauplius I

Copepodid

Number of Individuals (Start):

240

Sex:

unknown

Organism:

Lepeophtheirus salmonis

Target description

Gene or target symbol:

N protein LsRV-No9

Target type:

Viral

Primary target ID:

LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9

Fragment description

Fragment Producer Contact:

Aina-Cathrine Øvergård

Fragment Label ID:

FNo9

Fragment concentration:

1500.00ng/ul

Fragment length:

896bp

Sense sequence:

No9: ttctcccggaccgacatggaaacagagtttcatgttaatctatccaagcccggtattcaatcacaatatccatctgagtggttttctgctggaaacaaaaggcccagtgtgtccgtgaaggtcccaacaacgtatctgaaaaaggagtcaagagattacttttgtaatctcctaaacaactcattgacagtccccgaatggtcaacgatccaagggatggtctggctctcggagatgtatcctagtcaactgaaggggaatgatgtactagagtcattcgggataaagttaactgatgataaaaaccaggtgacggtcaaatcaattcttaactatgccgaggtagaggatcccactactacactcaaacccccagccaacaacaaaacagaatggactgaccaggagtactttgtcgggattacggcagtactggtcagttaccggtatcacagacttgtgcacgaggaggggagggaggccttactgagaagattagccggaatgctgacatctcaaacgctaagccttgcgtctattgttcggaaagccctcccagaacccggcgaagacatgaggaaggttatggcagcgctagatctccagttcaaccttggagatgggaaccccgaattcaaaagagtcaggatggggacagttcatctgcttcagagggatcagacatttctaagtgaagttcttgccatatctggttggtttgggaccgagtcctatgaaatgatcaaatggattttctctgccgaggtagcgaaggaggtcagagatagattttctcatgcagacgaggaagcaggacaacaattttcttactctgcctatatgattgatttcgggatttccaacagatctccttactcagtcaccgccaatccctt

Fragment Mixture Formula:

FNo9

Fragment Producer:

Aina-Cathrine Øvergård

Validation

Efficacy of Knock Down:

F>94.00%

Phenotype

Number of Individuals (End):

240

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Experiment Description:

Experiment Batch ID:

Start Date:

Experiment Contact:

Publication:

Developmental Stage CV:

Number of Individuals (Start):

Sex:

Organism:

Gene or target symbol:

Target type:

Primary target ID:

Fragment Producer Contact:

Fragment Label ID:

Fragment concentration:

Fragment length:

Sense sequence:

Fragment Mixture Formula:

Fragment Producer:

Efficacy of Knock Down:

Number of Individuals (End):

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