EMLSAT00000001458 cop + infection_b

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General

End Date: 

Wednesday, June 27, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000001458 in nauplia1 and infection of salmon with copepodids

Experiment Batch ID: 

Zhaoran_moult nauplia + infection

Start Date: 

Monday, June 18, 2018 - 14:00

Experiment Contact: 

Christiane Eichner
Sample

Developmental Stage CV: 

Nauplius1
Copepodid

Number of Individuals (Start): 

100

Sex: 

both

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

RAB1A

Target type: 

mRNA

External DBref: 

Primary target ID: 

EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Christiane Eichner

Antisense sequence: 

Fragment Label ID: 

F366

Fragment Batch ID: 

Fragment concentration: 

20.00ng/ul

Fragment length: 

412bp

Sense sequence: 

caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Fragment Mixture Formula: 

Fragment Producer: 

Licelab
Phenotype

Phenotype CV: 

Phenotype: 

no lice survived to the end of experiment, where control lice were mainly chalimus 2

Number of Individuals (End): 

0
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network middle old/moult yellowgreen cluster (most preserved in moulting + chitin genes). Experiment was done again to find out when lice disappear. Lice were sampled before moulting to chalimus 1 (1 fish) and after (2 fish). There were lice on the fish before the moult but no after.