Novel point mutation in the sodium channel gene of pyrethroid-resistant sea lice Lepeophtheirus salmonis (Crustacea: Copepoda)

Overview
TitleNovel point mutation in the sodium channel gene of pyrethroid-resistant sea lice Lepeophtheirus salmonis (Crustacea: Copepoda)
AuthorsFallang A, Denholm I, Horsberg TE, Williamson MS
TypeComparative Study
Media TitleDiseases of aquatic organisms
Volume65
Issue2
Year2005
Page(s)129-36
CitationFallang A, Denholm I, Horsberg TE, Williamson MS. Novel point mutation in the sodium channel gene of pyrethroid-resistant sea lice Lepeophtheirus salmonis (Crustacea: Copepoda). Diseases of aquatic organisms. 2005 Jun; 65(2):129-36.

Abstract

Knockdown resistance (kdr) to pyrethroid insecticides is caused by point mutations in the pyrethroid target site, the para-type sodium channel of nerve membranes. This most commonly involves alterations within the domain II (S4-S6) region of the channel protein, where several different mutation sites have been identified across a range of insect species. To investigate the possibility that a kdr-type mechanism is responsible for pyrethroid resistance in sea lice, a domain II region of the Lepeophtheirus salmonis sodium channel gene was PCR amplified and sequenced. To our knowledge, this is the first published sodium channel sequence from a crustacean. Comparison of sequences from a range of samples, including several individuals from areas in which control failures had been reported, failed to identify any of the mutations within this region that have previously been linked with resistance. Instead, a novel glutamine to arginine mutation, Q945R, in transmembrane segment IIS5 was consistently found in the samples from areas of control failure and may therefore be associated with resistance to pyrethroids in this species.

Author Details
Additional information about authors:
Details
1Anders Fallang
2Ian Denholm
3Tor Einar Horsberg
4Martin S Williamson
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN0177-5103
pISSN0177-5103
Publication Date2005 Jun
Journal AbbreviationDis. Aquat. Org.
LanguageEnglish
Language Abbreng
Publication TypeComparative Study
Journal CountryGermany
Publication TypeJournal Article
Publication TypeResearch Support, Non-U.S. Gov't
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DatabaseAccession
PMID: PMID:16060266