The screening of putative semiochemicals involved in the interaction between Lepeophtheirus salmonis copepodid and the Atlantic salmon could be used to develop methods to prevent infestation. A bioassay was designed to evaluate the attractiveness of Atlantic salmon for L. salmonis copepodids by counting the number of hooked parasites. A salmon was bathed in a tank with 60 copepodids during 45 min. The salmon was humanely killed and its body was scrubbed above a plastic bag. The content of the bag was filtered to count the number of hooked parasites. This procedure was tested at different water temperatures and for different body mass. The temperature significantly influenced the number of hooked copepodids (p < .05). Smolts presented significantly more hooked copepodids at a higher water temperature (24.6 at 13.8°C) compared to smolts at a lower temperature (18.6 at 6.1°C; p = .011). No correlation was found between the body mass and the number of hooked parasites (p = .65). This bioassay was used to successfully measure the attractiveness of Atlantic salmon for L. salmonis copepodids, making it an interesting tool to screen putative semiochemicals designed to prevent the infestation.