Investigation of autofluorescence in zooplankton for use in classification of larval salmon lice

Overview
TitleInvestigation of autofluorescence in zooplankton for use in classification of larval salmon lice
AuthorsNielsen JH, Pedersen C, Kiørboe T, Nikolajsen T, Brydegaard M, Rodrigo PJ
TypeJournal Article
Journal NameApplied optics
Volume58
Issue26
Year2019
Page(s)7022-7027
CitationNielsen JH, Pedersen C, Kiørboe T, Nikolajsen T, Brydegaard M, Rodrigo PJ. Investigation of autofluorescence in zooplankton for use in classification of larval salmon lice. Applied optics. 2019 Sep 10; 58(26):7022-7027.

Abstract

We present autofluorescence of six zooplankton species, including salmon lice (Lepeophtheirus salmonis), for the purpose of classification in marine environments. Using a 410 nm excitation wavelength, we find that all measured zooplankton species exhibit broad cyan fluorescence centered around 510-520 nm. Furthermore, salmon lice show an absence of red fluorescence from undigested chlorophyll, which is measured from the gut of the herbivorous zooplankton species. We show the capability to distinguish noneating species, including salmon lice, from algae-eating species using a dual-band analysis of the fluorescence spectra. This shows the potential of autofluorescence as an important signature in real-time monitoring and classification of salmon lice.

Author Details
Additional information about authors:
Details
1J H Nielsen
2C Pedersen
3T Kiørboe
4T Nikolajsen
5M Brydegaard
6P J Rodrigo
Properties
Additional details for this publication include:
Property NameValue
Journal CountryUnited States
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Elocation10.1364/AO.58.007022
DOI10.1364/AO.58.007022
Journal AbbreviationAppl Opt
Publication Date2019 Sep 10
eISSN1539-4522
ISSN1539-4522
Publication ModelPrint
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PMID: PMID:31503970