LsHSCARB - a putative intestinal heme receptor

Submitted by Erna Irene Heggland on 4 December, 2017 - 10:55

Phenotype CV:

General

Experiment Batch ID:

EMLSAT00000005382

Experiment Contact:

Erna Irene Heggland

Start Date:

21 March, 2017 - 10:10

End Date:

29 May, 2017 - 10:10

Experiment Description:

Knock-down of putative heme receptor

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis

Fragment description

Fragment length:

731bp

Fragment concentration:

600ng/ul

Sense sequence:

agcggataaactcgatggcttgccaaaaacagtaaaagatatttgttttgaaattataaataatggtaatgaagctctaggtattaaggccgaagatttatttattactgaagaagttgacaaaatcctatacacgggcttcgactctaagtctgctgctatctttgataaattggatacatttcttatgctcttgttagaagtcatacaagaatccttggagttggatataccaattaaagcaaaagactttgaaaacataatcaaaatcattagtcctgcacagctttcagaagggacttttgcttttttcaaaggtaaaaatgcaaccaaattgcagaattactataccattgaaaacggacggttcgataaagaaagttttatgaatattgttgagttcaatggtaagaataaattacctgaggcttggtggcctaatgtagccacaagtataacgggtcaactttcaagtgagggtggctcatgccatagaatctatggaacggatgggactcaatttccaccgtttttatttaacaagaaaaaatttcctttgtggatgtttgtgggagagctttgtagaacaatatatgtggagtttgagtccgaagtggaagtggaaggagggatcacagcctatcgttatggagtagggaagagagttttctcaatgagtaaccctgaaaacttttgttattgtcaagaatttttctcatgcgccaagcaaa

Fragment ID:

EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis

Fragment Label ID:

F289

Validation

Efficacy of Knock Down:

F>95%

Phenotype

Number of Individuals (End):

15

Phenotype:

Shorter egg-strings, 60% less heme, lower hatching rate

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Characterization of a putative intestinal heme scavenger.

Experiment terminated at control lice's 5 pair of egg-strings. We only start to see a phenotypic change at the 2nd and 3rd pair of egg-strings (not shown here). Treated lice had shorter egg-strings with a lower hatching rate, and the adult females had about 60% less heme (measured with fluorescence). Animals were disected into two poritons. We measured down-regulation by RNAi in the intestinal tissue and heme levels were measured in the remaining body tissues. See Materials&Methods for details.

C F4 (CopB2)

Submitted by Christiane Trösse on 24 July, 2015 - 16:11

Phenotype CV:

General

Experiment Batch ID:

C

Experiment Contact:

Christiane Trösse

Start Date:

14 August, 2012 - 09:00

End Date:

24 September, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>82%

Phenotype

Number of Individuals (End):

0

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Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F4 (CopB2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:44

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsCopB2

Fragment description

Fragment length:

566bp

Fragment concentration:

600ng/ul

Sense sequence:

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID:

F4

Primer Group:

b671

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>65%

Phenotype

Number of Individuals (End):

5

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Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

E F42

Submitted by Heidi Kongshaug on 12 March, 2013 - 10:38

Phenotype CV:

General

Experiment Batch ID:

E

Experiment Contact:

Frank Nilsen

Start Date:

15 October, 2012 - 10:19

End Date:

26 November, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000005071, EMLSAT00000005071-700918 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g13677_Rheb

Fragment description

Fragment length:

460bp

Fragment concentration:

600ng/ul

Sense sequence:

atggggcctcccaagtctcgtaaaattgccttaatgggagctgtttccgtgggcaaatcttctctggccatccaatttgtacaaggacaatttgtcgattcctacgaaccaacaatcgaaaatacatttaccaaaacagttaaaattaatagacaagaatatgaaatttgtcttgttgatacagcaggccaaaatgaatactctatctttccattggaatattctgtaggaatagatggatatgttcttgtatactccattgattcacctaaaagcttagaagtttgtcaaatcatacaggaaaaattaatagaattcacaggaaacccaaatgttcccattgtcttggttggcaataaaaatgatttgtatatggaaagatatgtttcctttgaagaaggcaaaagaagagctgaagaaatgaaagcagtgtttcttgagacctctgccaaggaaaacc

Fragment Label ID:

F42

Primer Group:

Rheb_f_b1063

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>60%

Phenotype

Number of Individuals (End):

25

Phenotype:

Low reproduction and most are missing blood in the gut

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Eggestrenger:

12.11 F42 ser ut som der ikke er noen sunne eggstrenge mer (har vært tidligere).

15.11 Lite blod i F42.