no visible phenotype

Nauplii I 7974-1

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Phenotype CV: 

General

Experiment Batch ID: 

N

Experiment Contact: 

Anna Komisarczuk

Start Date: 

1 February, 2016 - 14:40

End Date: 

10 February, 2016 - 01:41
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Developmental Stage CV: 

Nauplius I
Target description

Primary target ID: 

EMLSAG00000007974, EMLSAG00000007974-690740 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F272
Validation

Efficacy of Knock Down: 

F>80%
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no phenotype

EMLSAT00000009839_Var1Frag1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp2

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

17 November, 2017 - 11:17

End Date: 

23 November, 2017 - 11:47

Experiment Description: 

Knock Down in copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA
Fragment description

Fragment length: 

429bp

Fragment concentration: 

10ng/ul

Sense sequence: 

acctggtcagcaacgacttggcccacgagagcaagttaaccaaaatacggctttcattgatgcatctatgatttatgggaatgacaaatgtgactctattgatttacgacaaaacatcgatggtaggctgaacactacactacccagtcatggaagaggcaaacatcttatgcccaaggatagttccaataaggaatgtaaagcggcttcaggattttgtttcaaaggaggagatgctagagcctctgagcagcctggacttgctgctttacatactatttttctcagggaacataatcgtctcgtcaccaagctgaaaagagtcaatcctcattggagtgatgacgaactttattatgagggaaggcatattttatcagctgtgaatcaacacatcacttataatgagttccttcctcggatcattgg

Fragment Label ID: 

9839Fragment1

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>59%
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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000004874_Var2Frag2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp2

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

9 June, 2017 - 11:17

End Date: 

4 July, 2017 - 11:47

Experiment Description: 

Fish infested With KD copepodides
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA

External DBref: 

Fragment description

Fragment length: 

345bp

Fragment concentration: 

10ng/ul

Sense sequence: 

gcatgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatcttg

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

4874Fragment2

Fragment Mixture Formula: 

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>61%
Phenotype

Phenotype: 

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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000004874_Var2Frag1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp2

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

17 November, 2017 - 11:17

End Date: 

23 November, 2017 - 11:47

Experiment Description: 

Knock Down in copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA

External DBref: 

Fragment description

Fragment length: 

324bp

Fragment concentration: 

10ng/ul

Sense sequence: 

tcaagcgacgaggaattattgctactcaaggaagaaaattcttacttaatgaagagtacgctgactgatgatggaaaagaactccttcccaaagatggtaaaggtagttttgtgggaggagacgctagagctcttctaaccactggtttggcttcagttcataccttattcgttcgggaacataatcgaatatcaaggaaacttgctcagatgaatccttcatacacaaacgaaactgtttttcaagaaactcggcgaatattgggagcaatgatgcaaaatattgtttatagtgaatttcttccgacgttattaggggaaaaa

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

4874Fragment1

Fragment Mixture Formula: 

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>77%
Phenotype

Phenotype: 

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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000003179 cop + infection

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

network_moult nauplia + infection

Experiment Contact: 

Christiane Eichner

Start Date: 

9 May, 2018 - 09:00

End Date: 

1 June, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000003179 in nauplia1 and infection of salmon with copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

180

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000003179, EMLSAT00000003179-699026 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Csdc2

Target type: 

mRNA
Fragment description

Fragment length: 

386bp

Fragment concentration: 

20ng/ul

Sense sequence: 

Gcgtaaaagttgcgtacaatctgatattatattcgttctcattccgtgttcaccatttcttgcttttaagatggagagtgtcgtgagttcgactaccaaggatatgaagctcaacccaagcaccccatccccagaacggccctcaaatctcatgaagattccactcattcgaagaacacgctgcgattcatgctccgagaatgtactcagctccactacgaccactcctggaatcattgcggagtcccgtgacccaccagagacctatggtacaaggcgtacacggacagagtccacagcggggaagattgtggagaatctgcatgagggaattgtcaaatatttctgtcgatccagggggttcggattcatcacacccaataaac

Fragment Label ID: 

F365

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Christiane Eichner
Phenotype

Number of Individuals (End): 

45

Phenotype: 

no proper visible phenotype but maybe quicker development than controll
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network middle old/moult violet cluster (lethal + chitin genes)

EMLSAT00000004874_Var2Frag2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp1

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

9 June, 2017 - 11:17

End Date: 

4 July, 2017 - 11:47

Experiment Description: 

Fish infested With KD copepodides
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Copepodid
PreAdult
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA

External DBref: 

Fragment description

Fragment length: 

345bp

Fragment concentration: 

10ng/ul

Sense sequence: 

gcatgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatcttg

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

4874Fragment2

Fragment Mixture Formula: 

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>15%
Phenotype

Phenotype: 

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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000004874_Var2Frag1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp1

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

9 June, 2017 - 11:17

End Date: 

4 July, 2017 - 11:47

Experiment Description: 

Fish infested With KD copepodides
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Copepodid
PreAdult
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA

External DBref: 

Fragment description

Fragment length: 

324bp

Fragment concentration: 

10ng/ul

Sense sequence: 

tcaagcgacgaggaattattgctactcaaggaagaaaattcttacttaatgaagagtacgctgactgatgatggaaaagaactccttcccaaagatggtaaaggtagttttgtgggaggagacgctagagctcttctaaccactggtttggcttcagttcataccttattcgttcgggaacataatcgaatatcaaggaaacttgctcagatgaatccttcatacacaaacgaaactgtttttcaagaaactcggcgaatattgggagcaatgatgcaaaatattgtttatagtgaatttcttccgacgttattaggggaaaaa

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

4874Fragment1

Fragment Mixture Formula: 

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>20%
Phenotype

Phenotype: 

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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

ExpH - EMLSAT000000009293

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

ExpH

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

13 May, 2018 - 09:51

End Date: 

16 May, 2018 - 09:54

Experiment Description: 

Nauplia I was given dsRNA 8 days prior to infestation. Fish (around 100g) were infested with 80 cops/Fish and skin with and without lice were sampled 3 days after infestation.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

640

Developmental Stage CV: 

Copepodid
Copepodid
Target description

Primary target ID: 

EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Unknown

Target type: 

mRNA
Fragment description

Fragment length: 

331bp

Fragment concentration: 

20ng/ul

Sense sequence: 

attcttaatgaagaatctggtgagggaaatgtcacagtcaccgtggtagatacaatggatagaagttcctttgtcatgaactccgatgatgagagtgctggtcttccagttccccttagtgaagtcgtggaaacccccaacaatgactatataatgaataatcctatcgttttaaatgagaaaaacgatgttattctgaatgacgatgttattcttagtggtgaagaccttactctgattgaaaatcccactttatcattggatgatcttctagttgaagaagaagaaagtgatgatgttattttgaactaaatgtcacaatatctttctg

Fragment Label ID: 

E9293_V1+V2

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>95%
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Experiment H - Experiment to sheck immune modulatory effect of salivary gland proteins.

ExpH - EMLSAT000000006642

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

ExpH

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

13 May, 2018 - 09:51

End Date: 

16 May, 2018 - 09:54

Experiment Description: 

Nauplia I was given dsRNA 8 days prior to infestation. Fish (around 100g) were infested with 80 cops/Fish and skin with and without lice were sampled 3 days after infestation.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

640

Developmental Stage CV: 

Copepodid
Copepodid
Target description

Primary target ID: 

EMLSAT00000006642, EMLSAT00000006642-702489 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Unknown

Target type: 

mRNA
Fragment description

Fragment length: 

248bp

Fragment concentration: 

20ng/ul

Sense sequence: 

gcaaatccgggaaaagaaatccaagtgacatcatgttttttttttagttgttctactcaaaagtgccgatgtgctggaattgcaccgccaccagcaccagcccctgcaccagcgcctgcaccagcgcctgcacccgcccctgcaccagcccctgcaccagcgcctgcacctgcaccagcacctgcaccagcgcctgcaccagcaccaaagggaggaagaagaagaagagacgtacccatcgttttcga

Fragment Label ID: 

E6642new120318

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>98%
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Experiment H - Experiment to sheck immune modulatory effect of salivary gland proteins.

ExpH - EMLSAT000000010949

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

ExpH

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

13 May, 2018 - 09:51

End Date: 

16 May, 2018 - 09:54

Experiment Description: 

Nauplia I was given dsRNA 8 days prior to infestation. Fish (around 100g) were infested with 80 cops/Fish and skin with and without lice were sampled 3 days after infestation.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

640

Developmental Stage CV: 

Copepodid
Copepodid
Target description

Primary target ID: 

EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Trypsin

Target type: 

mRNA
Fragment description

Fragment length: 

446bp

Fragment concentration: 

14ng/ul

Sense sequence: 

gggacaaacctcggtcagcgataaaagagggagmaatgttttaaaacgtacaagactccaggttctccagaactctcatcagtattgtattaatggagcttctcttgggaaaccaccatggataaagcaaatgtgcgcctatgctgaaggaacagattcttgtggaggggatagtggaggmccacrcacattacctcaaaatgggagatgtgstctagtagggyttgtatcttatgggttggaatgtgccctgacttaccatgctggagtatatactcgtgtctctgaatatttgttttggataaaacatctagcaaaagatggaggctgtttcggtggagggaccgaaatgaaaccaaaatattgtgatatgcgctgtttgtggggacatgtcactgcaacatactacattactaataataggcatgaagttaagtgcgaccagg

Fragment Label ID: 

F233

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>93%
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Experiment H - Experiment to sheck immune modulatory effect of salivary gland proteins.

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