no visible phenotype

AQ F372 EMLSAG00000011562

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RAQ

Experiment Contact: 

Christiane Eichner

Start Date: 

16 April, 2018 - 09:00

End Date: 

23 May, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000011562
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000011562, EMLSAT00000011562-707409 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

EMLSAG00000011562
Fragment description

Fragment length: 

405bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ggttgcaggaaatgaagaagaggcatggattgagatttggaatctacgaggaggagctcctttctttcagcgttggcttccaackggaraatcctctctagaggctattgcattcggagccaaaggacgaatgtactccacgggtttgcacggatatatcctgtcacatgacattcagtctggagcagtatcaaagtcgcctgtgagttggggtgcttcatggtgcttggattatcatcctttgagcaagaggatcgcagttggtacggaagaaggtgttgtgtgcatattcagagaagagtcttcaggcgagattgtctatgaaaagtcacttgataaacaagaaggtcgaatcctatctcttgcttgggaacctctttctggagataagatggtaacgggctc

Fragment Label ID: 

F372

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

13

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000003179. No visible phenotype

AQ F374 EMLSAG00000011382

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RAQ

Experiment Contact: 

Christiane Eichner

Start Date: 

16 April, 2018 - 09:00

End Date: 

23 May, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000004269
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000011382, EMLSAT00000011382-707229 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

EMLSAG00000011382
Fragment description

Fragment length: 

482bp

Fragment concentration: 

600ng/ul

Sense sequence: 

ctgaaggatcctcagagcagcgattatscactcatttaggtccagcaccttttgattcagacaaasccgccgctattgaaagagaaagtcaagactattctattaaaataacattggacatggctagatctggattgtctccacgaaaagttcgagtgtatgccgatggaatatatgacttgtttcatcaaggacatgctagacaattgatgcaagcaaaaaatgtctttggacctcgaacggaggtttatctcctcgtagggtgtacaagtgacgagcttacaaataaaagaaaaggtaaaactgtaatgagtgaagaagaacgctatgaggccattcggcattgtcgctatgtggatgaggtagtgagagatgctccttgggaatgcgatgatgactttttgtcttttcataaaatagattttgtagctcatgacgaagctccatatgttacggagtctggaagtgatgtatatgccgcg

Fragment Label ID: 

F374

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

9

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000003179. No visible phenotype

AQ F373 EMLSAG00000004269

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RAQ

Experiment Contact: 

Christiane Eichner

Start Date: 

16 April, 2018 - 09:00

End Date: 

23 May, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000004269
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000004269, EMLSAT00000004269-700116 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment length: 

393bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gttgcgagtttgtttcctgatcgttcatcactttgtgctccctttttataataatctattatccctaaaagaaaatggcttttgtttactgtggattccttaaagagcctacgggtctcatgaagattttcttactctgcctcagcattatcaacgtatgcctagcccgtgctggatttcatttggttccttacattgagactgtagatgctcgatggctcgcttatgtaacatctggtggatatgttctcattctctcagtccttgttctctccatgatcttctcgaatgagatgtccatgagtgcggatattctctttagtttggttggaactgccctttatcttgccacaggaaccatggctgmtatggcctatgatgataccgaggatg

Fragment Label ID: 

F373

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

7

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000003179. No visible phenotype

AP F247 EMLSAG00000005706

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

RAP

Experiment Contact: 

Christiane Eichner

Start Date: 

6 March, 2018 - 09:00

End Date: 

10 April, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000005706
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

31

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment concentration: 

600ng/ul

Sense sequence: 

ATTTGAATGAACCTGCAGCAGCAACTCTTGGTGAGGATTTTCATATATCTTACATCTTTGGCTCATACCCTAAGCCAAAAATTGAAGACATAATATGGAGAATTAAGGGAACTGAGGATGTAAACATTGAGTTAAAAGCAAAAAACCCAAGGGATCCATCCGTATTTATGGATGGAAAATATATTTTGGATCCAATTATAAATGCTGGAGATCAAATGTATAGCATTGGTTTTACTATTTCAAACGTTACTGAAGAGGATTTTGCAAGAACTTACGATGTGAAAATTAGTAATCGTAAAAAACATTCCAACGATCTTATCCTTTCAACAGTTCACACTTTTGACATACAAACGTCAACTGCAGGTGGATCCAGTGACACTGGATCATCAAGTACTGTTACAGGGATCGTTATATCCATTGCGCTAATTCTTTTGCTCATTACTCTTGTCACGATCCTCGTAATGACTTATATGTACAAAAAGTGGTGTTTTGCTCCCAAAGATCCTCATTCCAAGCCAGGGTATGATGGAGACGTTGAACA

Fragment Label ID: 

F247

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Phenotype: 

No visible phenotype
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Knock-down of EMLSAT00000003179. No visible phenotype

HCP1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AG

Experiment Contact: 

Erna Irene Heggland

Start Date: 

16 August, 2016 - 10:31

End Date: 

23 September, 2016 - 10:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000003292, EMLSAT00000003292-699139 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment length: 

693bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tcactttttgcgggaccgtggtcggatattaatggacgaaaagccattatagttttatcttgttttggctatgtctttaataacgtcatatatttattgaataccgtcttttttttcgaactaaaagcagagtatctcttgtttgagtgtctacaagtaaagtaataagccacagttgaaggtgtaatgtttattatacatgttcattttatcttttaaggactttactggtggctatgtagcctttttttttgcctgtcactcttatataactgatataactgatgatagttcaagaacaaaacgaatggcctttctcgatggaatctttccaatcgggttccttatgggattaggtctaagtgggatcattaaaaaaaagttgggatttacttactgcttttcactagctatcgttacctcctggtcatccattctctacacaatattcttcattaaagaacgaaatagctctgaagatcaagttgataacacggatgataattcaacaactcagagtgctaataataaggttaaaaaggagccgatctggaaaatgtttaagattcaacactttcaagcgggggtgaaatcaacattgaaaaggcgtgaacatgatctaaaaaagtacttgctctttttgattctgatctttatgcttgagttattcctctacggagggaaaggctctgt

Fragment Label ID: 

F290
Validation

Efficacy of Knock Down: 

F>80%
Phenotype

Phenotype: 

No difference in heme levels
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No difference in heme levels

LsHSCARB on free living stages

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

F289

Experiment Contact: 

Erna Irene Heggland

Start Date: 

26 July, 2016 - 09:55

End Date: 

2 August, 2016 - 10:00

Experiment Description: 

Knock-down evaluation of primers
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

300

Developmental Stage CV: 

Nauplius I
Copepodid
Target description

Primary target ID: 

EMLSAT00000005382, EMLSAT00000005382-701229 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

731bp

Fragment concentration: 

10ng/ul

Sense sequence: 

AGCGGATAAACTCGATGGCTTGCCAAAAACAGTAAAAGATATTTGTTTTGAAATTATAAATAATGGTAATGAAGCTCTAGGTATTAAGGCCGAAGATTTATTTATTACTGAAGAAGTTGACAAAATCCTATACACGGGCTTCGACTCTAAGTCTGCTGCTATCTTTGATAAATTGGATACATTTCTTATGCTCTTGTTAGAAGTCATACAAGAATCCTTGGAGTTGGATATACCAATTAAAGCAAAAGACTTTGAAAACATAATCAAAATCATTAGTCCTGCACAGCTTTCAGAAGGGACTTTTGCTTTTTTCAAAGGTAAAAATGCAACCAAATTGCAGAATTACTATACCATTGAAAACGGACGGTTCGATAAAGAAAGTTTTATGAATATTGTTGAGTTCAATGGTAAGAATAAATTACCTGAGGCTTKGTGGCCTAATGTAGCCACAAGTATAACGGGKCAACTTTCAAGTGAGGGTGGCTCATGCCATAGAATCTATGGAACGGATGGGACTCAATTTCCACCGTTTTTATTTAACAAGAAAAAATTTCCTTTGTGGATGTTTGTGGGAGAGCTTTGTAGAACAATATATGTGGAGTTTGAGTCCGAAGTGGAAGTGGAAGGAGGGATCACAGCCTATCGTTATGGAGTAGGGAAGAGAGTTTTCTCAATGAGTAACCCTGAAAACTTTTGTTATTGTCAAGAATTTTTCTCATGCGCCAAGCAAA

Fragment Label ID: 

F289
Validation

Efficacy of Knock Down: 

F>99%
Phenotype

Number of Individuals (End): 

250

Phenotype: 

none
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Testing the efficiency of RNAi fragment in freeliving stages. Non-parasitic stage is not feeding and therefore no phenotype was expected.

Rhabdovirus - No9+127 prod of virus free strain

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp6

Experiment Contact: 

Aina-Cathrine Øvergård
Frank Nilsen

Start Date: 

16 January, 2017 - 14:11

Experiment Description: 

Test if it is possible to cure lice for two rhabdovirus simultaneously. Used lice taken from Hardanger fjord that was infected with both LsRV-No9 and No127 strains. Treatment given at Nauplius I and pre-adult stage. Copepods (80/fish) were given to 6 fish, and sampled at 25 days post infestation. Pre-adult lice were given 600 ng/ul dsRNA by injection and sampled at 55 dpi.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

480

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9
LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127

Gene or target symbol: 

N protein LsRV-No9
N protein LsRV-No127

Target type: 

Viral
Viral
Fragment description

Fragment length: 

896bp

Fragment concentration: 

3000ng/ul

Sense sequence: 

No9: ttctcccggaccgacatggaaacagagtttcatgttaatctatccaagcccggtattcaatcacaatatccatctgagtggttttctgctggaaacaaaaggcccagtgtgtccgtgaaggtcccaacaacgtatctgaaaaaggagtcaagagattacttttgtaatctcctaaacaactcattgacagtccccgaatggtcaacgatccaagggatggtctggctctcggagatgtatcctagtcaactgaaggggaatgatgtactagagtcattcgggataaagttaactgatgataaaaaccaggtgacggtcaaatcaattcttaactatgccgaggtagaggatcccactactacactcaaacccccagccaacaacaaaacagaatggactgaccaggagtactttgtcgggattacggcagtactggtcagttaccggtatcacagacttgtgcacgaggaggggagggaggccttactgagaagattagccggaatgctgacatctcaaacgctaagccttgcgtctattgttcggaaagccctcccagaacccggcgaagacatgaggaaggttatggcagcgctagatctccagttcaaccttggagatgggaaccccgaattcaaaagagtcaggatggggacagttcatctgcttcagagggatcagacatttctaagtgaagttcttgccatatctggttggtttgggaccgagtcctatgaaatgatcaaatggattttctctgccgaggtagcgaaggaggtcagagatagattttctcatgcagacgaggaagcaggacaacaattttcttactctgcctatatgattgatttcgggatttccaacagatctccttactcagtcaccgccaatccctt No127: ggagccatcggaggttatgacctgggtgactcttgattccgtctcgactgagatcttgaagaccgtcagagatactgaggaaatttattgtcctttctcttatgccccctatatgatggatctcgggatatcagctaagtctccgtattcggtcacagaaaatcctttgtctcacctatggattcatgtgacaggagtaatattaaataacccgcgatctatgaatgcccggattatcggggagaccacatttcatcaggtaattttgtcctccgccctcctggcttatcggtgcgcaacaagtcctttattgagtcaaaagttcagtggacaatccgacctcagaggggtagaagagagtattagggtcttgtcggagtcaagttcatatttctcaaacactgaggatcttgatctggaagacgatccacaacaaaacactcccatggcatacgtcgcttggtgtgacaacaagtggaaccgtcgactttttacatttgtagactccgcaatgctaggattgacacggcccctt

Fragment Label ID: 

FNo9
FNo127

Fragment Mixture Formula: 

FNo9+FNo127

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>98%
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Rhabdovirus - No9+127 prod of virus free strain

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp5

Experiment Contact: 

Aina-Cathrine Øvergård
Frank Nilsen

Start Date: 

16 January, 2017 - 14:11

Experiment Description: 

Test if it is possible to cure lice for two rhabdovirus simultaneously. Used lice taken from Hardanger fjord that was infected with both LsRV-No9 and No127 strains. Treatment given at Nauplius I and pre-adult stage. Copepods (80/fish) were given to 6 fish, and sampled at 25 days post infestation. Pre-adult lice were given 600 ng/ul dsRNA by injection and sampled at 75 dpi. The offspring of these females were treated with dsRNA at Nauplius I and pre-adult stage as described above, and adult lice were sampled at 55 dpi.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

unknown

Number of Individuals (Start): 

480

Developmental Stage CV: 

Nauplius I
Adult
Target description

Primary target ID: 

LsRhabNo9.nucleoprotein.g, KJ958535.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No 9
LsRhabNo127.nucleoprotein.g, KJ958536.nucleoprotein.g (gene) Lepeophtheirus salmonis rhabdovirus No127

Gene or target symbol: 

N protein LsRV-No9
N protein LsRV-No127

Target type: 

Viral
Viral
Fragment description

Fragment length: 

896bp

Fragment concentration: 

3000ng/ul

Sense sequence: 

>No9: ttctcccggaccgacatggaaacagagtttcatgttaatctatccaagcccggtattcaatcacaatatccatctgagtggttttctgctggaaacaaaaggcccagtgtgtccgtgaaggtcccaacaacgtatctgaaaaaggagtcaagagattacttttgtaatctcctaaacaactcattgacagtccccgaatggtcaacgatccaagggatggtctggctctcggagatgtatcctagtcaactgaaggggaatgatgtactagagtcattcgggataaagttaactgatgataaaaaccaggtgacggtcaaatcaattcttaactatgccgaggtagaggatcccactactacactcaaacccccagccaacaacaaaacagaatggactgaccaggagtactttgtcgggattacggcagtactggtcagttaccggtatcacagacttgtgcacgaggaggggagggaggccttactgagaagattagccggaatgctgacatctcaaacgctaagccttgcgtctattgttcggaaagccctcccagaacccggcgaagacatgaggaaggttatggcagcgctagatctccagttcaaccttggagatgggaaccccgaattcaaaagagtcaggatggggacagttcatctgcttcagagggatcagacatttctaagtgaagttcttgccatatctggttggtttgggaccgagtcctatgaaatgatcaaatggattttctctgccgaggtagcgaaggaggtcagagatagattttctcatgcagacgaggaagcaggacaacaattttcttactctgcctatatgattgatttcgggatttccaacagatctccttactcagtcaccgccaatccctt >No127: ggagccatcggaggttatgacctgggtgactcttgattccgtctcgactgagatcttgaagaccgtcagagatactgaggaaatttattgtcctttctcttatgccccctatatgatggatctcgggatatcagctaagtctccgtattcggtcacagaaaatcctttgtctcacctatggattcatgtgacaggagtaatattaaataacccgcgatctatgaatgcccggattatcggggagaccacatttcatcaggtaattttgtcctccgccctcctggcttatcggtgcgcaacaagtcctttattgagtcaaaagttcagtggacaatccgacctcagaggggtagaagagagtattagggtcttgtcggagtcaagttcatatttctcaaacactgaggatcttgatctggaagacgatccacaacaaaacactcccatggcatacgtcgcttggtgtgacaacaagtggaaccgtcgactttttacatttgtagactccgcaatgctaggattgacacggcccctt

Fragment Label ID: 

FNo9
FNo127

Fragment Mixture Formula: 

FNo9+FNo127

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>100%
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AP F364 (F168+F169)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AP

Experiment Contact: 

Christiane Eichner

Start Date: 

6 March, 2018 - 09:00

End Date: 

10 April, 2018 - 09:00

Experiment Description: 

Mvp1 and Mvp2/3 knock-down
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN53_5145, UN52_5383
LsMvp1, LsMvp2/3

Target type: 

UN53_5145
UN52_5383
Fragment description

Fragment length: 

576bp

Fragment concentration: 

600ng/ul

Sense sequence: 

>F169 cacatggagacgaagaagtgcgattggagagagatccgtttccattatatccaggagaagagttaaaggtcaaggtcacaccattggcaatcgttcatacctcaaatgcccttttattaaaagttataagaaatttcacggacgaaaatgatgttaaacgtgtggctggagatttatttttgtttgagggacctggaacttatattccaaggaaagaggtcgaggttcttaaaaccattacagcacaagttatccatgaaaatgaagcccttaaattaagtgcaagtcgtgaaaccatagatcgatccggaattaaacacgttgcaggtgaagaatggctcataagaaagcctggagcttatttgcctttggcttatgaaaatattgtggaagttgttaaggcacatataccccttccagatgtcgcaattttggtaagagcaaaggcaactttcaaggacaagtttggtgttgagagaaaaaatggagacaaatacttgattacctttgaagatacatcagcattcattcctgaggtacaagaagaaataattggcacagtggatgctatcacac >F168 cctcccacccgaatggtcgtaatacctccaggatcttattgtgttgtatcaaaccctgttgtaacaaaagatggtactattgagaaagatcgctttggccaaattaagttatctcatggagatgaagaaattcgattaaaaagagatccttttccattatatccaggagagaaattgaaagttgaagtgacgcctttaacaatcgtacattcatcaagtgcacttcttttaaaagttatcagaaattttactgatgaagacaaaactgagcgtsttgctggagatttatacttgttcgaaggtccaggaacttattttcctagaaaagaggtggaagttgttaaaactattactgctacagtcatacatgagaatgaagctttaaaactgagtgctgctcgtgaaactttagacagatctggttgtaagagagtggcaggggagg

Fragment Label ID: 

F364

Fragment Mixture Formula: 

F169+F168

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>68%
Phenotype

Number of Individuals (End): 

15

Phenotype: 

normal
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Testing the fragment for Mvp1 knock-down.

AP F245

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AP

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

7 March, 2018 - 13:26

End Date: 

10 April, 2018 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

31

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000005706, EMLSAT00000005706-701553 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

T10_8281
Fragment description

Fragment length: 

458bp

Fragment concentration: 

600ng/ul

Sense sequence: 

aggaagcgaggagtgctgccatatccggccctgatagtttggacgtcatgtttgactgctaccgcgttagtcgactaagctatgcatctgttgttacatatgatggttgttttgtagacaatmccaacggagatatgaatcgtttaactggaatattttccgccacagtcgagggkatttatcaattttctttcacagctaagtacgttagctccagtagaggacgatttggagcttggagtgatatttatgtgaacgatactgtgattgcagactcacaaagggagtacaatgctaggagcaatttagagacggagtcttcaactcacacagttcttgtgtattttccaatacggatgaatcaccaagtcaaagtacaattcaataaagatggttcaagttatatacactcagatggagatcatgatgtccatttcactggtagaagagttgggccc

Fragment Batch ID: 

AP

Fragment Label ID: 

F245

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

24
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Image file: 

File RAP114.tif

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