BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0010507 "negative regulation of autophagy" evidence=IEA] [GO:0021702 "cerebellar Purkinje cell differentiation" evidence=IEA] [GO:0031175 "neuron projection development" evidence=IEA] [GO:0050885 "neuromuscular process controlling balance" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 InterPro:IPR015880 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 SMART:SM00355 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 OrthoDB:EOG70KGNN TreeFam:TF106426 GeneTree:ENSGT00740000114947 EMBL:AADN03006099 Ensembl:ENSGALT00000005344 PRO:PR:E1C4H7 Uniprot:E1C4H7)

HSP 1 Score: 785.408 bits (2027), Expect = 0.000e+0
Identity = 474/1281 (37.00%), Postives = 701/1281 (54.72%), Query Frame = 0

Query: 2162 SYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGN-IIYIWD-MEDSEENCNQIPQLSPIIYLCW----SPNSEIEILLAAR-QNG--------TSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLH---YVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            S +   A G  DG + +      ++  I+  D      +S      + W P+GK L+      ++ +W  M  S    + +P  + +  + W       S++++LLA    NG          I++T  + S+ S+       +G+    +  + K    L G       +    D   L SG     GG++NIWS+   SV++++ IG G I    WI    +A     +++V ++N  +E    N I+A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L K     +   +  L    E +  + + +  W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  V+CG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P+++P L  +   DIAVG +H LALS +G V+ WG N  GQLG       +EP +I  L GK I QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++   L   S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   V++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAP+VWK +  I    +DLE+VDL Y ++L+   ++  S   +     ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ IM  RL YAI +CR+IDMD+YML+RN
Sbjct: 3580 SEDKPFAVGYSDGKLLIGTKEPLEKGEIVLLDAHKDTIVS------MKWDPTGKILLTCAKEEMVKLWAYMGGSWRRLHSLPHPAFVNGIAWCGLPGKGSKLQLLLATGCHNGLVCVWTVPQDIAVTLQTSSAYSEGWWDQETSGQDGYRKAVEAKCVFQLRGHITPVRTVAFSPDGLALVSGGL---GGLMNIWSLRDGSVLQSVVIGSGAIQTAVWIPEVGVAACCNRSKDVLVVNCTSEWISSNHILAT-CRTALKQQGVLGLNMAPCMRAFLERLPIMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPSTWSPTEWAWLECFSTTIKAAEALAKGAQFPESFTVPDLEPVPEDELTLLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPVAAPSFSQAQQVVCGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTADGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTVAVSTDGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDILCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQVPVLSGVFIEDIAVGAEHTLALSSTGDVYAWGSNSEGQLGLGHTNHVREPTLITVLQGKNIRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDAIPPQY-GALKEVSIHTVRARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGVVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPMVWKQLCCIPLILEDLEEVDLLYVQTLNSILHIEDSGITEENFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLIMAERLRYAINNCRSIDMDNYMLSRN 4841          

HSP 2 Score: 337.806 bits (865), Expect = 4.749e-91
Identity = 243/763 (31.85%), Postives = 370/763 (48.49%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHPLS--STVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQG-------------------------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFP--SPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W  P     LS  + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL     N S  +N +   K +Q  +G                               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I ++S G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS +   L +     +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D  ++  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPASGPGLSLCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFL-KGVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMAL-----NASAVVNTMEKTKLLQSPEGMISFDCFMSILMQMRRSLGSSADRSQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGVSIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSHLRSFLEH-----YCDKINSEIPPLPFPSSREHHSFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-SVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 174.481 bits (441), Expect = 1.546e-41
Identity = 130/369 (35.23%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VDI++G  H LALS    V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGVSIQQISAGTSHSLAWTALP 737          

HSP 4 Score: 171.014 bits (432), Expect = 1.461e-40
Identity = 117/387 (30.23%), Postives = 191/387 (49.35%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HT+ VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ +  +S   I +++ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    +I  I AG   S AWT
Sbjct: 3967 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPVAAPSFSQAQQVVCGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTADGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTVAVSTDGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDILCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQVPVLSGVFIEDIAVGAEHTLALSSTGDVYAWGSNSEGQLGLGHTN-HVREPTLITVLQGKNIRQISAGRCHSAAWT 4350          

HSP 5 Score: 113.235 bits (282), Expect = 5.209e-23
Identity = 141/648 (21.76%), Postives = 278/648 (42.90%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKL----LNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNSKSSDLDFT-NIQRTSYNME--------------------FNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLE--------AIYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S      +     + +      SKK      NE   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +  +       I+     M+                    F+ + +  CI+++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  LK     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1735 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGISQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQAGVMLMASFGEEEVEEHEAESKKADSNGENEKRDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCCSGVPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEMKLQKQGESEDEDENLPIQEVSFDPEKAQCCIVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----THCINKKMMERLNKIKTCLKEAGQKLKKSRSIQSREENEVREEKENKEEEKGKHNRHGLADLSELQLKMLCVEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2350          

HSP 6 Score: 91.6633 bits (226), Expect = 1.739e-16
Identity = 80/278 (28.78%), Postives = 125/278 (44.96%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQ-TKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+I +L G F   V  G+  S  LT  G+++ +G G    LG G+ + T   P+ I  L    +  ++ G  H              CL  +        +V+  GN+        +S   I  PKK+  L+  ++  I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGSSEATALRPKLIEELAATRIVDISIGDSH--------------CLALSH-----DNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGVSIQQISAGTSHSLA 732          

HSP 7 Score: 67.3958 bits (163), Expect = 3.628e-9
Identity = 85/379 (22.43%), Postives = 163/379 (43.01%), Query Frame = 0

Query: 1964 TDPMEGLYFKNEKELCCSFGPDIEVLKVILYEAKNKLPSFLAL-CNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFH-LSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRIL-------IIDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEI-----EILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            T+   G++ K  K + C     +  L   +    +  PS   L   +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  + +  +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H   N+ I   +  +D+ G L +  +  +L  H  ++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S +S  ++ WS SGKYL  +   ++ IW +   +   +  P    +  L W           E+LL  R +G+                        +S+T L+W S     A+GY +G++L+
Sbjct: 3237 TERSRGIHSKTSKPISC-----LAYLSTAVGCLASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKAEF--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTIP--QTLADMGGDLRKCSF-IKLEAHQKQVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVLNKLEGDSEESLGSPSDLSLSLICWSISGKYLAGATEKMVNIWQVNGGKGLLDLQPHW--VSALAWPEEGPAAAWKGELLLIGRMDGSLGLIEVVDISTMHRIELEHCYRKDVSVTCLAWFSEDKPFAVGYSDGKLLI 3596          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 OrthoDB:EOG70KGNN GeneTree:ENSGT00740000114947 EMBL:AAEX03016202 Ensembl:ENSCAFT00000042764 Uniprot:J9NWD8)

HSP 1 Score: 783.867 bits (2023), Expect = 0.000e+0
Identity = 444/1109 (40.04%), Postives = 640/1109 (57.71%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNL---KDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D  L    +  W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  +V  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK I QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3014 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPDVGVAACSNRSKDVLVVNCTAEWAAANHVLAT-CRTALKQQGVLGLNMAPCMRAFLERLPVMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELLFLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTNHVREPTLVTVLQGKNIRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDAVPPQYGALR-EVSIHTARARLRLLYHFSDLMYSSWRLLNLSPNNQSSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFIGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4107          

HSP 2 Score: 172.555 bits (436), Expect = 5.245e-41
Identity = 119/387 (30.75%), Postives = 191/387 (49.35%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ I  ++   I +V+ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    +I  I AG   S AWT
Sbjct: 3233 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTN-HVREPTLVTVLQGKNIRQISAGRCHSAAWT 3616          

HSP 3 Score: 108.997 bits (271), Expect = 7.708e-22
Identity = 137/648 (21.14%), Postives = 277/648 (42.75%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNN----LRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLEA--------IYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S      + S       +     + V      E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +                     K  +L+  +       + F+ + +  C++++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1043 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQAGVLLMASFGEGEGEEGEEEEKKVDSSGETEKKDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKSQKQGELEEEDENLPIQEVSFDPEKAQCCLVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKESKEEEKGKHNRHGLADLSEPQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 1658          

HSP 4 Score: 64.3142 bits (155), Expect = 2.748e-8
Identity = 59/215 (27.44%), Postives = 101/215 (46.98%), Query Frame = 0

Query:  546 GKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWT-MRKGKGCFHW--PYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
            G+CG G ++  +  PK+   LD   I  I AG + S AWT + + +    W  PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:    2 GQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHNFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 210          

HSP 5 Score: 60.077 bits (144), Expect = 5.159e-7
Identity = 80/349 (22.92%), Postives = 152/349 (43.55%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRILI-------IDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIW---------DMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +      +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW         D++    +    P+  P     WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L+
Sbjct: 2531 ASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTTP-QTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPAT--AWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLL 2865          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Probable E3 ubiquitin-protein ligase HERC1" species:9606 "Homo sapiens" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005086 "ARF guanyl-nucleotide exchange factor activity" evidence=TAS] [GO:0005737 "cytoplasm" evidence=TAS] [GO:0005794 "Golgi apparatus" evidence=IEA] [GO:0005829 "cytosol" evidence=IEA] [GO:0006810 "transport" evidence=IEA] [GO:0010507 "negative regulation of autophagy" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0021702 "cerebellar Purkinje cell differentiation" evidence=IEA] [GO:0031175 "neuron projection development" evidence=IEA] [GO:0043087 "regulation of GTPase activity" evidence=TAS] [GO:0050885 "neuromuscular process controlling balance" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 UniPathway:UPA00143 INTERPRO:IPR000408 Pfam:PF00415 GO:GO:0005829 GO:GO:0005794 GO:GO:0016020 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0010507 GO:GO:0006810 GO:GO:0050885 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 GO:GO:0031175 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 eggNOG:COG5021 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 GO:GO:0021702 GO:GO:0005086 EMBL:AC118274 EMBL:U50078 EMBL:AC073167 EMBL:BC040929 PIR:S71752 RefSeq:NP_003913.3 RefSeq:XP_005254799.1 RefSeq:XP_005254800.1 UniGene:Hs.210385 PDB:4O2W PDBsum:4O2W ProteinModelPortal:Q15751 SMR:Q15751 BioGrid:114439 IntAct:Q15751 MINT:MINT-8330052 STRING:9606.ENSP00000390158 PhosphoSite:Q15751 DMDM:296434522 PaxDb:Q15751 PRIDE:Q15751 Ensembl:ENST00000443617 GeneID:8925 KEGG:hsa:8925 UCSC:uc002amp.3 CTD:8925 GeneCards:GC15M063900 HGNC:HGNC:4867 HPA:HPA049749 MIM:605109 neXtProt:NX_Q15751 PharmGKB:PA29242 HOGENOM:HOG000168589 HOVERGEN:HBG053919 InParanoid:Q15751 KO:K10594 OMA:PIAQAKH OrthoDB:EOG70KGNN TreeFam:TF106426 SignaLink:Q15751 GeneWiki:HERC1 GenomeRNAi:8925 NextBio:33556 PRO:PR:Q15751 ArrayExpress:Q15751 Bgee:Q15751 CleanEx:HS_HERC1 Genevestigator:Q15751 Uniprot:Q15751)

HSP 1 Score: 783.482 bits (2022), Expect = 0.000e+0
Identity = 445/1109 (40.13%), Postives = 641/1109 (57.80%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKD--VNLKD-IVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D  V L D   W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 KI+      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  ++  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK + QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3757 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPEVGVAACSNRSKDVLVVNCTAEWAAANHVLAT-CRTALKQQGVLGLNMAPCMRAFLERLPMMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELVFLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKIDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVIIEDVAVGAEHTLALASNGDVYAWGSNSEGQLGLGHTNHVREPTLVTGLQGKNVRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDTVPPQYGALR-EVSIHTVRARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4850          

HSP 2 Score: 330.487 bits (846), Expect = 8.586e-89
Identity = 242/760 (31.84%), Postives = 372/760 (48.95%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW----SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKA------------VQLAQG--------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W    SGP     L + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL  +    ++    ++ ++             +Q+ +               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I +VS G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLS--LCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLK-GVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMALGASAVVHTMEKGKLLSSQEGMISFDCFMTILMQMRRSLGSSADRSQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDVSIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHSFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 174.096 bits (440), Expect = 1.939e-41
Identity = 121/389 (31.11%), Postives = 188/389 (48.33%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-----------NTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ I+   G+ +KKV    +FS+AL+  G ++ +G+              N    +P     I  I +V+ G  H + +  N  VY+WG N+ G+ GLG T+  V+ P     L   ++  I AG   S AWT
Sbjct: 3976 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKIDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVI--IEDVAVGAEHTLALASNGDVYAWGSNSEGQLGLGHTN-HVREPTLVTGLQGKNVRQISAGRCHSAAWT 4359          

HSP 4 Score: 173.326 bits (438), Expect = 3.075e-41
Identity = 129/369 (34.96%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VD+++G  H LALS    V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDVSIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGIAIQQISAGTSHSLAWTALP 737          

HSP 5 Score: 110.538 bits (275), Expect = 2.830e-22
Identity = 138/648 (21.30%), Postives = 277/648 (42.75%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISN----NLRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLE--------AIYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S     ++ S             + V      E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    IV +LFS + +  W +                     K  +L+  +       + F+ + +  C++++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1736 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQTGVLHMASFGEGEQEDGEEEEKKVDSSGETEKKDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQIVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKLQKQGELEEEDENLPIQEVSFDPEKAQCCLVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKESKEEEKGKHTRHGLADLSELQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2351          

HSP 6 Score: 92.4337 bits (228), Expect = 8.183e-17
Identity = 81/278 (29.14%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQ-TKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+I +L G F   V  G+  S  LT  G+++ +G G    LG G+ + T   P+ I  L    +  V+ G  H              CL  +        +V+  GN+        +S   I  PKK+  L+ +    I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGSSEATALRPKLIEELAATRIVDVSIGDSH--------------CLALSH-----DNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLA 732          

HSP 7 Score: 61.6178 bits (148), Expect = 1.681e-7
Identity = 80/349 (22.92%), Postives = 152/349 (43.55%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRIL-------IIDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIW---------DMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +      +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW         D++    +    P+  P     WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L+
Sbjct: 3274 ASNAPSAAKLLVQLCTQNLISAATGVNLTTVDDSIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTT-LQTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPAT--AWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLL 3608          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0010507 GO:GO:0050885 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 GO:GO:0031175 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 GO:GO:0021702 CTD:8925 KO:K10594 OMA:PIAQAKH OrthoDB:EOG70KGNN TreeFam:TF106426 GeneTree:ENSGT00740000114947 EMBL:CU550694 EMBL:CU582937 RefSeq:XP_001927286.4 UniGene:Ssc.96208 UniGene:Ssc.97542 Ensembl:ENSSSCT00000005038 GeneID:100153537 KEGG:ssc:100153537 Uniprot:F1S098)

HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0
Identity = 445/1109 (40.13%), Postives = 641/1109 (57.80%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKD--VNLKD-IVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D  V L D   W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  +V  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK + QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3755 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPDVGVAACSNRSKDVLVVNCTAEWAAANHVLAT-CRTALKQQGVLGLNMAPCMRAFLERLPMMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFSVPDLEPVPEDELVFLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTNHVREPTLVTVLQGKNVRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDAVPPQYGALR-EVSIHTARARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4848          

HSP 2 Score: 332.028 bits (850), Expect = 2.981e-89
Identity = 244/765 (31.90%), Postives = 370/765 (48.37%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW----SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQG-------------------------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W    SGP     L + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL       S  +N +  +K +   +G                               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I ++S G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLS--LCNDVIHTATEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLK-GVTIPNSGADILGRRLASELLLGLAAQRGSLRYLLEWIEMALGA-----SAVVNSMEKSKLLSSQEGMISFDCFMTILMQMRRSLGSSADRSQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHNFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 173.711 bits (439), Expect = 2.431e-41
Identity = 130/369 (35.23%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VDI++G  H LALS    V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGIAIQQISAGTSHSLAWTALP 737          

HSP 4 Score: 171.4 bits (433), Expect = 1.118e-40
Identity = 118/387 (30.49%), Postives = 191/387 (49.35%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ I  ++   I +V+ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    ++  I AG   S AWT
Sbjct: 3974 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTN-HVREPTLVTVLQGKNVRQISAGRCHSAAWT 4357          

HSP 5 Score: 112.464 bits (280), Expect = 8.758e-23
Identity = 141/648 (21.76%), Postives = 278/648 (42.90%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKL----LNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLEA--------IYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S      +L   F          KKL      E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +                     K  +L+  +       + F+ + +  CI+++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1736 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQA--GVLLMASFGEGEEDEEEEKKLDSSGETEKRDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKLQKQGELEEEDENLPIQEVSFDPEKAQCCIVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKESKEEEKGKHNRHGLADLSEPQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2349          

HSP 6 Score: 91.6633 bits (226), Expect = 1.387e-16
Identity = 80/278 (28.78%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQ-TKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+I +L G F   V  G+  S  LT  G+++ +G G    LG G+ + T   P+ I  L    +  ++ G  H              CL  +        +V+  GN+        +S   I  PKK+  L+ +    I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGSSEATALRPKLIEELAATRIVDISIGDSH--------------CLALSH-----DNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLA 732          

HSP 7 Score: 60.4622 bits (145), Expect = 4.332e-7
Identity = 80/347 (23.05%), Postives = 153/347 (44.09%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEKXAI-----YITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRIL-------IIDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQ-LSPIIY------LCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +      +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW +   +   +  P  +S + +        WS  S  E+LL  R +G+                        +SIT ++W S     A+GY +G++L+
Sbjct: 3272 ASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPGFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYEKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTTP-QTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPSTAWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSITCIAWFSEDRPFAVGYFDGKLLL 3606          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0010507 "negative regulation of autophagy" evidence=IEA] [GO:0021702 "cerebellar Purkinje cell differentiation" evidence=IEA] [GO:0031175 "neuron projection development" evidence=IEA] [GO:0050885 "neuromuscular process controlling balance" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0010507 GO:GO:0050885 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 GO:GO:0031175 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 GO:GO:0021702 OMA:PIAQAKH OrthoDB:EOG70KGNN TreeFam:TF106426 GeneTree:ENSGT00740000114947 EMBL:DAAA02028744 EMBL:DAAA02028745 EMBL:DAAA02028746 Ensembl:ENSBTAT00000061038 ArrayExpress:F1MNS0 Uniprot:F1MNS0)

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 442/1110 (39.82%), Postives = 641/1110 (57.75%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKD---VNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEM-MPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D   + + +  W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  +V  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK + QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM   QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3752 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPDVGVAACSNRSKDVLVVNCTAEWTAANHVLAT-CRTALKQQGILGLNMAPCMRAFLERLPMMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQHCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELVLLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTNHVREPTLVTVLQGKNVRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDTVPPQYGALR-EVSIHTARARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4846          

HSP 2 Score: 330.872 bits (847), Expect = 6.344e-89
Identity = 244/765 (31.90%), Postives = 370/765 (48.37%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW----SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQG-------------------------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W    SGP     L + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL       S  +N +  +K +   +G                               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I ++S G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLS--LCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLK-GVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMAL-----GASAVVNSMEKSKLLSSQEGMISFDCFMTILMQMRRSLGSSADRSQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDTPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHNFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 173.711 bits (439), Expect = 2.217e-41
Identity = 130/369 (35.23%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VDI++G  H LALS    V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGIAIQQISAGTSHSLAWTALP 737          

HSP 4 Score: 171.785 bits (434), Expect = 1.019e-40
Identity = 118/387 (30.49%), Postives = 191/387 (49.35%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ I  ++   I +V+ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    ++  I AG   S AWT
Sbjct: 3971 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTN-HVREPTLVTVLQGKNVRQISAGRCHSAAWT 4354          

HSP 5 Score: 110.153 bits (274), Expect = 4.231e-22
Identity = 138/649 (21.26%), Postives = 279/649 (42.99%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKLLN-----EPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLEA--------IYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y  +     LS   +++L ++L + + N L    + L+        +   K ++     E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +                     K  +L+  +       + F+ + +  CI+++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1736 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTYADK-----LSPKVVQSLLDLLCSQLKNLLSQAGVLLMASFGEGEEDEEGKKIDSSGETEKRDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKLQKQGELEEEDENLPIQEVSFDPEKAQCCIVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKENKEEEKGKHSRHGLADLSEPQLRTLCLEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2348          

HSP 6 Score: 92.0485 bits (227), Expect = 1.341e-16
Identity = 80/278 (28.78%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQ-TKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+I +L G F   V  G+  S  LT  G+++ +G G    LG G+ + T   P+ I  L    +  ++ G  H              CL  +        +V+  GN+        +S   I  PKK+  L+ +    I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGSSEATALRPKLIEELAATRIVDISIGDSH--------------CLALSH-----DNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLA 732          

HSP 7 Score: 60.4622 bits (145), Expect = 3.918e-7
Identity = 79/347 (22.77%), Postives = 153/347 (44.09%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRIL-------IIDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQ-LSPIIY------LCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H   +     +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW +   +   +  P  +S + +        WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L+
Sbjct: 3269 ASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAH-DRDNQTAPQTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPSTAWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLL 3603          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0010507 "negative regulation of autophagy" evidence=IEA] [GO:0021702 "cerebellar Purkinje cell differentiation" evidence=IEA] [GO:0031175 "neuron projection development" evidence=IEA] [GO:0050885 "neuromuscular process controlling balance" evidence=IEA] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0010507 GO:GO:0050885 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 GO:GO:0031175 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 GO:GO:0021702 OMA:PIAQAKH TreeFam:TF106426 GeneTree:ENSGT00740000114947 EMBL:AAEX03016202 Ensembl:ENSCAFT00000026962 Uniprot:E2QYQ5)

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 444/1110 (40.00%), Postives = 640/1110 (57.66%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNL---KDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEM-MPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D  L    +  W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  +V  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK I QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM   QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3758 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPDVGVAACSNRSKDVLVVNCTAEWAAANHVLAT-CRTALKQQGVLGLNMAPCMRAFLERLPVMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELLFLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTNHVREPTLVTVLQGKNIRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDAVPPQYGALR-EVSIHTARARLRLLYHFSDLMYSSWRLLNLSPNNQSSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFIGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4852          

HSP 2 Score: 323.553 bits (828), Expect = 1.118e-86
Identity = 243/766 (31.72%), Postives = 371/766 (48.43%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW----SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQG-------------------------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNN-GELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W    SGP     L + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL       S  +N +  +K +   +G                               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+N G+LG GDT  +Y PK++    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I ++S G  H+ + + +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLS--LCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLK-GVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMAL-----GASAVVNSMEKSKLLSSQEGMISFDCFMTILMQMRRSLGSSADRTQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKMALSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNVGKLGHGDTNRVYKPKVVEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDISIGDSHVWLFVMDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHNFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 904          

HSP 3 Score: 172.17 bits (435), Expect = 7.476e-41
Identity = 119/387 (30.75%), Postives = 191/387 (49.35%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ I  ++   I +V+ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    +I  I AG   S AWT
Sbjct: 3977 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTN-HVREPTLVTVLQGKNIRQISAGRCHSAAWT 4360          

HSP 4 Score: 165.622 bits (418), Expect = 6.458e-39
Identity = 127/370 (34.32%), Postives = 176/370 (47.57%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT-------------------------KINFAWRESVT---------KICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                           N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VDI++G  HV        V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKMALSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNVGKLGHGDTNRVYKPKVVEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDISIGDSHVWLFVMDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGIAIQQISAGTSHSLAWTALP 738          

HSP 5 Score: 109.383 bits (272), Expect = 7.800e-22
Identity = 137/648 (21.14%), Postives = 277/648 (42.75%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNN----LRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLEA--------IYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S      + S       +     + V      E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +                     K  +L+  +       + F+ + +  C++++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1737 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQAGVLLMASFGEGEGEEGEEEEKKVDSSGETEKKDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKSQKQGELEEEDENLPIQEVSFDPEKAQCCLVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKESKEEEKGKHNRHGLADLSEPQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2352          

HSP 6 Score: 60.077 bits (144), Expect = 5.760e-7
Identity = 80/349 (22.92%), Postives = 153/349 (43.84%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLW----KDNEYKRILI---IDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIW---------DMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +      +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W    K    ++  +   ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW         D++    +    P+  P     WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L+
Sbjct: 3275 ASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTTP-QTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPAT--AWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLL 3609          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:Herc1 "HECT and RLD domain containing E3 ubiquitin protein ligase family member 1" species:10116 "Rattus norvegicus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0010507 "negative regulation of autophagy" evidence=ISO] [GO:0021702 "cerebellar Purkinje cell differentiation" evidence=ISO] [GO:0031175 "neuron projection development" evidence=ISO] [GO:0050885 "neuromuscular process controlling balance" evidence=ISO] Pfam:PF00632 InterPro:IPR017986 InterPro:IPR000569 InterPro:IPR001680 InterPro:IPR003877 InterPro:IPR015943 Pfam:PF00400 Pfam:PF00622 PROSITE:PS50082 PROSITE:PS50237 PROSITE:PS50294 SMART:SM00119 SMART:SM00320 RGD:1306366 INTERPRO:IPR000408 Pfam:PF00415 Gene3D:2.130.10.10 SUPFAM:SSF50978 InterPro:IPR019775 PROSITE:PS00678 GO:GO:0016567 InterPro:IPR008985 SUPFAM:SSF49899 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 SUPFAM:SSF56204 InterPro:IPR001870 InterPro:IPR018355 SMART:SM00449 PROSITE:PS50188 OrthoDB:EOG70KGNN GeneTree:ENSGT00740000114947 EMBL:AABR06055430 EMBL:AABR06055431 Ensembl:ENSRNOT00000023646 NextBio:35572302 ArrayExpress:D4A3S5 Uniprot:D4A3S5)

HSP 1 Score: 718.383 bits (1853), Expect = 0.000e+0
Identity = 417/1082 (38.54%), Postives = 607/1082 (56.10%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNEMD--NVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP----------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKD---VNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTY----VACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPP 3315
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N I+A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LCS P                 + W WL  F+ ++     L   N     E       + + +D   + + +  W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +      VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  +V  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK I QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L +        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ WLW  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP    P+   C F +RLPP
Sbjct: 2210 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPEVGVAACSNRSKDVLVVNCTAEWASANHILAT-CRTALKQQGVLGLNMAPCMRAFLERLPMMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCSPPVPPHHQNCPPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELVLLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPATAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQAWHKVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTNHVREPTLVTVLQGKNIRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDAVPPQYGALR-EVSIHTVRARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFSPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEICVDVLKKVVRYREVDEQHQLVQWLWRTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPHASPPSQALCLFHVRLPP 3276          

HSP 2 Score: 165.622 bits (418), Expect = 6.078e-39
Identity = 119/391 (30.43%), Postives = 191/391 (48.85%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGS----VSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G     V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ I  ++   I +V+ G  H + +     VY+WG N+ G+ GLG T+  V+ P  + +    +I  I AG   S AWT
Sbjct: 2429 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPATAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQAWHKVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVVIEDVAVGAEHTLALASTGDVYAWGSNSEGQLGLGHTN-HVREPTLVTVLQGKNIRQISAGRCHSAAWT 2816          

HSP 3 Score: 107.842 bits (268), Expect = 1.888e-21
Identity = 106/483 (21.95%), Postives = 215/483 (44.51%), Query Frame = 0

Query: 1140 YKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLEA--------IYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    +V +LFS + +  W +                     K  +L+  +       + F+ + +  CI+++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  ++     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct:  343 FRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQVVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKLQKQGELEEEDENLPIQEVSFDPEKAQCCIVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIRESGQKLKKSRSVQSREENEMREEKENKEEEKGKHNRHGLADLSEPQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 804          

HSP 4 Score: 62.003 bits (149), Expect = 1.262e-7
Identity = 85/370 (22.97%), Postives = 160/370 (43.24%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRILI-------IDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIW---------DMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEIL-----------VLQIDQRKESI 2305
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P + + T +E K    P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H   +     +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW         D++    +    P+  P     WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L           ++ ID  KE++
Sbjct: 1727 ASNTPSAAKLLVQLCTQNLISAATGVNLTTVDDPIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNY-DKTEIEKKG---PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAH-DRDNQTAPQTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPAT--AWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLMGTKEPLEKGGIVLIDAHKETL 2082          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC2 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005743 "mitochondrial inner membrane" evidence=IEA] [GO:0006281 "DNA repair" evidence=IEA] [GO:0007283 "spermatogenesis" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0020037 "heme binding" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0032183 "SUMO binding" evidence=IEA] Pfam:PF00632 InterPro:IPR000433 InterPro:IPR000569 InterPro:IPR001199 InterPro:IPR010606 Pfam:PF00173 Pfam:PF00569 Pfam:PF06701 PROSITE:PS01357 PROSITE:PS50135 PROSITE:PS50237 PROSITE:PS50255 PROSITE:PS51416 SMART:SM00119 SMART:SM00291 INTERPRO:IPR000408 Pfam:PF00415 GO:GO:0005634 GO:GO:0005743 GO:GO:0008270 GO:GO:0006281 GO:GO:0007283 GO:GO:0020037 Gene3D:2.60.120.260 InterPro:IPR008979 SUPFAM:SSF49785 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 Gene3D:3.10.120.10 SUPFAM:SSF55856 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 InterPro:IPR004939 Pfam:PF03256 PROSITE:PS51284 SUPFAM:SSF56204 Gene3D:2.30.30.30 InterPro:IPR021097 InterPro:IPR014722 Pfam:PF11515 OrthoDB:EOG70KGNN GeneTree:ENSGT00740000115443 OMA:GHGTDVH TreeFam:TF320636 EMBL:AADN03001643 Ensembl:ENSGALT00000027018 PRO:PR:E1BW48 Uniprot:E1BW48)

HSP 1 Score: 460.685 bits (1184), Expect = 3.177e-128
Identity = 314/1021 (30.75%), Postives = 501/1021 (49.07%), Query Frame = 0

Query: 2416 AFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDV--------NLKDI-----VWTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGF--VWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A +K     +  Q++YE   +  G QL++S F + L  LA  L L+T+ C     +W W   +  +      L+KR        L  LF DE+ K +        N+  +     V+  E D  L++W    P DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  I G Q  FA+  +G LYA G G+ G+LG+G ++     TP +   + H  +                    V+SWG+ EDGKLGHGN   C RP++I SL G     +A G +HSA +T  G +FT+G G  GRLGHG+ + +  P+ + +L+GY V  +ACG        L      ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ P+++  L+   ++ IA G  H +  ++ G   V+ WG N  GQLG+ +    + P ++  L GK I +++ G  H+ AW++            +KP +    P  +P E+  L  +P  S    L  L  + E+    + ++ L   +D     P        ++  D +R  L          + + +TM      R +GP V + R+             P G+ S+F Q+  ++    P  L LP+R WKVK VGE+ DD GG + ++I+EMC+EL   +  LL  TPNG  + G+N+D +LLN    S + M  F FLGVL G+A+RT  P+++NLA  VWK +  +  T  DL +VD  +   L Y+  +     E +++ LP   F   + SG+++ L   S+   ++ +N+ EYV+ A+N R  E   QV AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ ++P+  +I W WE++E F+N  + LFL FV GR+RLP    D   R  +++V      P++ LP + TCFF L+LP Y+ K+++  +L YAI  C++ID D Y
Sbjct: 3820 ALVKGLPEALQRQFDYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTP------LPRLFLDEVAKKIRELMADNENMDVLHECHDVFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVS--TPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLFTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTDLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLG-HGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGLGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWST------------SKPANAGKLPTQVPMEYNHLQEIPIISLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGP--------SVGFDTLRGILISQGKEAAFRKVVQATMVRD---RQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAAKSLLHMNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLP---FTVPNASGQDIQL--SSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPTASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDY 4803          

HSP 2 Score: 168.318 bits (425), Expect = 9.871e-40
Identity = 111/370 (30.00%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW--------------------------RESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++ V++ G     QL  L  S I  P ++++    +   V  G +  FA+   G +YACGE + G+LGLG S     +   +T L ++ +  V                  FSWG+G+DGKLGH +   C +P++I +L       +A G+SHSA +T  G+++T+G G+ GRLGHG+  T+  P+ +  L G+ V  VACG        L    + F+W                           + V +I  G  FSL LT SG V+  G   +  +  + ++++++ P+ +  L  + +V +AVG  H LA++ +G V+ WG N  GQ GN +    ++P +++ L G+ I +++ G +HS AWT+
Sbjct: 2962 RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLG-HGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTT 3330          

HSP 3 Score: 157.532 bits (397), Expect = 2.043e-36
Identity = 105/284 (36.97%), Postives = 151/284 (53.17%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP------IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-----WNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR-----LDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            LAL+ + +V+SWG G  GRLGHGD      PK IS+L        ++ I+ G  ++AAIT +G LYTWG G+ GRLGH       +P LV  L    V  VSCG+    TL V+++G QVWS+G G+ G+LG+G +    TPKLI     + + KV   ++FS+AL+  G ++ WGK         T   V  PK +  +    + +V+ G  H + + E+  V+SWG N   +C     +L++  P+      LD   I GI  GP QS+AW+      C  W
Sbjct:  509 LALSANGEVFSWGCGDGGRLGHGDTVPLEEPKLISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENG-QVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDIVKVRCGSQFSIALTKDGQVYTWGKGDNQRLGHGTEEHVRYPKLLEGLKGKKVIDVAAGSTHCLALTEDSEVHSWGSND--QC-QHFDTLRITKPEPAALPGLDTKHIVGIACGPAQSFAWS-----SCSEW 783          

HSP 4 Score: 151.369 bits (381), Expect = 1.220e-34
Identity = 93/267 (34.83%), Postives = 145/267 (54.31%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            +ALT D K++SWG G  G+LGH  + + + P+ I +L    I  I+ G  H+AAIT  G LYTWG G  GRLGH D      P +VK L  H V  V+CG++   TL ++ +G+ V+S+G G+ G+LG+G +     P+ I   +G  + ++    +FSLAL+ SG +W WGK  +       +     P+ +  +    I +V+ G  H + + +   VY+WG N +G+ G G T++  K    + L+   I  +  G + S AWT
Sbjct: 3064 MALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGL-VFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWT 3329          

HSP 5 Score: 139.043 bits (349), Expect = 7.687e-31
Identity = 90/273 (32.97%), Postives = 144/273 (52.75%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI--MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILEN--RSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ + +VYSWG    G+LGHG++   + P+ I SL  I  + I+AG  H+A IT  G L+TWG+G  GRLGH D      P LV+ L  + V  ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I+    + + KV   ++FS+AL+ SG ++ WGK  +       +     P+ +  +    +  ++TG  H +   E+    VY+WG N  G+ G G T+  ++ P+    L    I+ +  G   + AW+  K
Sbjct: 4057 LALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLFTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDT-VWSWGDGDYGKLGRGGSDGCKVPMKIDSLTDLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGLGEVYTWGDNDEGQLGDGTTN-AIQRPRLVAALQGKKINRVACGSAHTLAWSTSK 4327          

HSP 6 Score: 124.02 bits (310), Expect = 2.378e-26
Identity = 83/265 (31.32%), Postives = 136/265 (51.32%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFA----TFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPPMPTNFNNSSPTKPRHNIGRPNSLP 2880
            VFSWG G+ G+LGHG+T     PK+IS+LSG  A      +A G+++SA +T +G+++T+G G+ GRLGHG+ + +  P  ++ L+G  V  V+CG                   G   +L +T +G V+  G+  +  +    S+   ++PK I KL+++  V +  G    +AL+K G V+ WG     +LG+ +    + P ++E L GK ++ ++AG  H  A         W S     +F+    TKP      P +LP
Sbjct:  517 VFSWGCGDGGRLGHGDTVPLEEPKLISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGS------------------GDAQTLAVTENGQVWSWGDGDYGKLGRGGSD-GCKTPKLIEKLQDLDIVKVRCGSQFSIALTKDGQVYTWGKGDNQRLGHGTEEHVRYPKLLEGLKGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKP-----EPAALP 757          

HSP 7 Score: 101.679 bits (252), Expect = 1.567e-19
Identity = 66/208 (31.73%), Postives = 100/208 (48.08%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSS------VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLV------------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            V+  G G  G+L      ++P   PK   +  G  +      + CG   + A+   G LY  G G+YG+LG G+S+D  ++  +VT L    ++                   V+SWGDG+ GKLG G ++ C+ PK+I  L       V  G+  S  LT  G+++T+G GD  RLGHG  +  + P+ +  L+G  V  VA G  H
Sbjct:  517 VFSWGCGDGGRLGH--GDTVPLEEPKLISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQ-TIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDIVKVRCGSQFSIALTKDGQVYTWGKGDNQRLGHGTEEHVRYPKLLEGLKGKKVIDVAAGSTH 721          

HSP 8 Score: 85.5001 bits (210), Expect = 1.081e-14
Identity = 71/259 (27.41%), Postives = 126/259 (48.65%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLG--HGDKKDYNFPKE-ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            +++  + + L+      +Y WG+   G+LG   G K     P E +++L P+  I  G     A+T DG LY  G G+ GRLG         PTL++++ +V     +V+ G +H L +S +G +V+S+G   +G+LG G+      P++I    G+ +  + A    S  ++ +GDL+ WGK  +       +     PK + ++     +     +G    + + ++ +V+SWG   YGK G G  S   K P ++D
Sbjct: 3942 MNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLI-GGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG-EVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLFTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRG-GSDGCKVPMKID 4197          

HSP 9 Score: 79.7221 bits (195), Expect = 6.127e-13
Identity = 50/165 (30.30%), Postives = 80/165 (48.48%), Query Frame = 0

Query: 2586 TFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLV----------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
            T A+  NG +++ G+G YGKLG G SD     TP ++  L   ++V                 V++WG G++ +LGHG  E  R PK++  L G     VA G++H   LT+  ++ ++G+ D+ +       TK  P  +  L    +  +ACG   +   FAW
Sbjct:  618 TLAVTENGQVWSWGDGDYGKLGRGGSDG--CKTPKLIEKLQDLDIVKVRCGSQFSIALTKDGQVYTWGKGDNQRLGHGTEEHVRYPKLLEGLKGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPAALPGLDTKHIVGIACGPAQS---FAW 777          

HSP 10 Score: 75.0998 bits (183), Expect = 1.747e-11
Identity = 69/259 (26.64%), Postives = 123/259 (47.49%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLG--HGDK-KDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGH--KDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            A T   KV+ WG     +LG   G K K  +F + +S+L  ++ ++ G     A+T++G +Y  GE + GRLG     G V  +P  +  L N      +V  G +H + ++ DG +++S+G G++G+LG         P+LI       ++ +   +  S A++ SG+L+ WG   +       NT+   PK +  +    +  V+ G      + + +   V+SWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2958 AATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALN-VVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVP-IPRQITALSNYVVKKVAVHSGGRHAMALTVDG-KIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRG-GSEGCNIPQNIERLNGQGVC 3212          

HSP 11 Score: 70.8626 bits (172), Expect = 2.891e-10
Identity = 41/111 (36.94%), Postives = 59/111 (53.15%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGY--LYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTL 433
            F +ALT+   VY+WG G + RLGHG       P+++  L    ++ I+ G  H    T DG   +YTWG+   G+LG  DG  + +  P LV  L    +  V+CG+ HTL
Sbjct: 4213 FSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGLGEVYTWGDNDEGQLG--DGTTNAIQRPRLVAALQGKKINRVACGSAHTL 4321          

HSP 12 Score: 68.5514 bits (166), Expect = 1.693e-9
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTL 433
            F LALT+   V++WG G + RLGHG       P+ +  L    I+ ++ G  H  A+T  G +Y WG+   G+ G+    V+  PTLV+ L    +  V+CG+ H++
Sbjct: 3220 FSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSV 3326          

HSP 13 Score: 66.6254 bits (161), Expect = 6.519e-9
Identity = 58/198 (29.29%), Postives = 88/198 (44.44%), Query Frame = 0

Query: 2661 SGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            +G+     A G   +A +  + K+F +G  D+ +LG       K P    +L    V  VA G   +K  FA                    G V+ CG +    + +  S+  +  P++I  L N V   +AV  GG H +AL+  G ++ WG    G+LG+ S     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 2944 TGSLVRKKAAGLESAATI--RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGG---SKSLFA----------------VTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITS 3120          

HSP 14 Score: 62.7734 bits (151), Expect = 7.336e-8
Identity = 36/123 (29.27%), Postives = 61/123 (49.59%), Query Frame = 0

Query:  288 TGSSCTYVIHTNGKVSCWKDNHFSNV--------HFPSPI-----IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWG 395
            +G + T  +  NG+V  W D  +  +          P  I     +D    +    F +ALT+D +VY+WG G + RLGHG ++   +PK +  L    ++ ++AG  H  A+T D  +++WG
Sbjct:  613 SGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDIVKVRCGSQFSIALTKDGQVYTWGKGDNQRLGHGTEEHVRYPKLLEGLKGKKVIDVAAGSTHCLALTEDSEVHSWG 735          

HSP 15 Score: 62.7734 bits (151), Expect = 9.432e-8
Identity = 61/201 (30.35%), Postives = 94/201 (46.77%), Query Frame = 0

Query:  389 GYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVS-CGAQHTLI-VSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLT--SLKVKNPKRLDIYDIDGICAG 574
            G +Y WG    G+LG  +G    VPT  + L  +  V   G + TL  V+ DG ++++ G G  G LG G T  + TP L+     V +KKV   +  +  LALS  G+++ WG+           +    P+ I S+  I   +++ GG H   I     +++WG   YG+ G G +   LK K  + L  Y +  I  G
Sbjct: 3957 GTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADG-KLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLFTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACG 4156          

HSP 16 Score: 50.8322 bits (120), Expect = 3.860e-4
Identity = 34/91 (37.36%), Postives = 49/91 (53.85%), Query Frame = 0

Query: 2772 NMNIQSPKKIPKL--ENMVDIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKG----ILQISAGKNHSAAWTS 2854
            N +   P+ +  L   N+V IA    G H LALS +G V+ WGC   G+LG+      +EP +I  LSGK     ++ I+ G  +SAA T+
Sbjct:  479 NSDTLGPQLVQSLASRNIVKIAAHSDGHHYLALSANGEVFSWGCGDGGRLGHGDTVPLEEPKLISALSGKQAGKHVVHIACGSTYSAAITA 569          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:Herc2 "hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 2" species:10090 "Mus musculus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;IBA] [GO:0005634 "nucleus" evidence=ISO;IBA] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005743 "mitochondrial inner membrane" evidence=IDA] [GO:0005856 "cytoskeleton" evidence=IEA] [GO:0006281 "DNA repair" evidence=ISO] [GO:0006974 "cellular response to DNA damage stimulus" evidence=ISO] [GO:0007283 "spermatogenesis" evidence=IMP] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016567 "protein ubiquitination" evidence=ISO] [GO:0016874 "ligase activity" evidence=IEA] [GO:0020037 "heme binding" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=ISO] [GO:0032183 "SUMO binding" evidence=ISO] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0046872 "metal ion binding" evidence=IEA] Pfam:PF00632 InterPro:IPR000433 InterPro:IPR000569 InterPro:IPR001199 InterPro:IPR010606 Pfam:PF00173 Pfam:PF00569 Pfam:PF06701 PROSITE:PS01357 PROSITE:PS50135 PROSITE:PS50237 PROSITE:PS50255 PROSITE:PS51416 SMART:SM00119 SMART:SM00291 UniPathway:UPA00143 MGI:MGI:103234 INTERPRO:IPR000408 Pfam:PF00415 GO:GO:0005634 GO:GO:0005814 GO:GO:0005743 GO:GO:0008270 GO:GO:0006281 GO:GO:0007283 GO:GO:0020037 Gene3D:2.60.120.260 InterPro:IPR008979 SUPFAM:SSF49785 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 Gene3D:3.10.120.10 SUPFAM:SSF55856 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 InterPro:IPR004939 Pfam:PF03256 PROSITE:PS51284 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 GO:GO:0032183 Gene3D:2.30.30.30 InterPro:IPR021097 InterPro:IPR014722 Pfam:PF11515 OrthoDB:EOG70KGNN CTD:8924 GeneTree:ENSGT00740000115443 KO:K10595 OMA:GHGTDVH HOVERGEN:HBG081598 TreeFam:TF320636 InterPro:IPR006624 SMART:SM00706 EMBL:AF061529 EMBL:AF071173 EMBL:AC102121 EMBL:AC102150 EMBL:AK141515 EMBL:AK148361 EMBL:AK161826 EMBL:AK162270 EMBL:AK162708 EMBL:AK220338 EMBL:BC044667 EMBL:BC054829 RefSeq:NP_034548.2 RefSeq:XP_006540700.1 RefSeq:XP_006540701.1 RefSeq:XP_006540702.1 UniGene:Mm.20929 ProteinModelPortal:Q4U2R1 SMR:Q4U2R1 BioGrid:200274 PhosphoSite:Q4U2R1 PaxDb:Q4U2R1 PRIDE:Q4U2R1 Ensembl:ENSMUST00000076226 Ensembl:ENSMUST00000164095 GeneID:15204 KEGG:mmu:15204 UCSC:uc009hdv.1 InParanoid:Q4U2R1 ChiTaRS:HERC2 NextBio:287753 PRO:PR:Q4U2R1 Bgee:Q4U2R1 Genevestigator:Q4U2R1 Uniprot:Q4U2R1)

HSP 1 Score: 458.373 bits (1178), Expect = 1.519e-127
Identity = 311/1019 (30.52%), Postives = 497/1019 (48.77%), Query Frame = 0

Query: 2416 AFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKK-------DVNLKDI------VWTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A +K     +  Q+EYE   +  G QL++S F + L  LA  L L+T+ C     +W W   +  +      L+KR        L  LF DE+ K       D    D+      ++  E D  L++W    P DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  I G Q  FA+  +G LYA G G+ G+LG+G ++     TP +   + H  +                    V+SWG+ EDGKLGHGN   C RP++I SL G     VA G +HSA +T  G ++T+G G  GRLGH + + +  P+ + +L+G+ V  +ACG        L      ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ P+++  L+   ++ IA G  H +  ++ G V+ WG N  GQLG+ +    + P ++  L GK + +++ G  H+ AW++            +KP      P  +P E+  L  +P  +    L  L  + E+    + ++ L   +D     P        ++  D +R  L          + + +TM      R +GP V + R+             P G+ S+F Q+  ++    P  L LP+R WKVK VGE+ DD GG + ++I+E+C+EL   +  LL  TPNG  + G+N+D YLLN    + V    F FLGVL G+A+RT  P+++NLA  VWK +  +  T  DL +VD  +   L Y+  +     E +++ LP   F   S SG+++ L   S+   ++ +N+ EYV+ A+N R  E   QV AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ ++PS  ++ W WE++E F+N  + LFL FV GR+RLP    D   R  +++V      P++ LP + TCFF L+LP Y+ K+++  +L YAI  C++ID D Y
Sbjct: 3817 ALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTP------LPRLFLDEVAKKIRELMADSESMDVLHESHSIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVS--TPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLG-HGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKVNRVACGSAHTLAWST------------SKPASAGKLPAQVPMEYNHLQEIPIIALRNRLLLLHHISELFCPCIPMFDLEGSLDETGLGP--------SVGFDTLRGILISQGKEAAFRKVVQATMVRD---RQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEICEELQNGLTPLLIVTPNGRDESGANRDCYLLNPATRAPVHCSMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMSLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLP---FTVPSASGQDIQL--SSKHTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPSASLVQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDY 4798          

HSP 2 Score: 171.4 bits (433), Expect = 1.164e-40
Identity = 114/370 (30.81%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW--------------------------RESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++ V++ G     QL  L  S I  P ++++    +   V  G +  FA+   G +Y+CGE + G+LGLG S     +   +T L S+ +  V                  FSWG+G+DGKLGH +   C +P++I +L       +A G+SHSA LT  G+++T+G G+ GRLGHG+  T+  P+ +  L G+ V  VACG        L    + F+W                           + V +I  G  FSL LT SG V+  G   +  +  + S+++++ P+ +  L  + +V +AVG  H LA++ SG V+ WG N  GQ GN +    ++P +++ L G+ I +++ G +HS AWT+
Sbjct: 2959 RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLG-HGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTT 3327          

HSP 3 Score: 154.836 bits (390), Expect = 1.380e-35
Identity = 110/308 (35.71%), Postives = 158/308 (51.30%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP------IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-----WNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK-----RLDIYDIDGICAGPTQSYAWTMRKGKGCFHW------PYSNEPDAKTFSQVSDIL 610
            LAL    +VYSWG G  GRLGHGD      PK IS+         ++ I+ G  ++AAIT +G LYTWG G+ GRLGH       +P LV  L    V  V+CG+    TL V+++G QVWS+G G+ G+LG+G +    TPKLI     + + KV   ++FS+AL+  G ++ WGK         T   V  PK +  +    + +V+ G  H + + E+  V+SWG N   +C     +L+V  P+      LD   I GI  GP QS+AW+      C  W      P+  +  + TF Q+  +L
Sbjct:  509 LALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENG-QVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSND--QC-QHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQSFAWS-----SCSEWSIGLRVPFVVDICSMTFEQLDLLL 807          

HSP 4 Score: 150.984 bits (380), Expect = 2.068e-34
Identity = 106/334 (31.74%), Postives = 167/334 (50.00%), Query Frame = 0

Query:  282 DVEKIETGSSCTYVIHTNGKV-SCWKDNH------FSNVHFPSPIIDASLS---------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            +V ++  GS   + +   GKV SC +  +       S+   P P    +LS                 ALT D KV+SWG G  G+LGH  + + + P+ I +L    I  I+ G  H+AA+T  G LYTWG G  GRLGH D      P +VK L  H V  V+CG++   TL ++ +G+ V+S+G G+ G+LG+G +     P+ I   +G  + ++    +FSLAL+ SG +W WGK  +       +     P+ +  +    I +V+ G  H + + ++  VY+WG N +G+ G G T++  K    + L+   I  +  G + S AWT
Sbjct: 2994 NVVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGL-VFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWT 3326          

HSP 5 Score: 149.828 bits (377), Expect = 4.111e-34
Identity = 90/271 (33.21%), Postives = 145/271 (53.51%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI--MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ + +VYSWG    G+LGHG++   + P+ I SL  I  + ++AG  H+A +T  G LYTWG+G  GRLGH D      P LV+ L  H V  ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I+   G+ + KV   ++FS+AL+ SG ++ WGK  +       +     P+ +  +    +  ++TG  H +   E+  VY+WG N  G+ G G T+  ++ P+    L    ++ +  G   + AW+  K
Sbjct: 4054 LALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDT-VWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTN-AIQRPRLVAALQGKKVNRVACGSAHTLAWSTSK 4322          

HSP 6 Score: 124.02 bits (310), Expect = 2.586e-26
Identity = 81/265 (30.57%), Postives = 135/265 (50.94%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFA----TFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPPMPTNFNNSSPTKPRHNIGRPNSLP 2880
            V+SWG G+ G+LGHG+T     PK+IS+ SG  A      +A G+++SA +T +G+++T+G G+ GRLGHG+ + +  P  ++ L+G  V  VACG                   G   +L +T +G V+  G+  +  +    S+   ++PK I KL+++  + +  G    +AL+K G V+ WG     +LG+ +    + P ++E L GK ++ ++AG  H  A         W S     +F+    TKP      P +LP
Sbjct:  517 VYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGS------------------GDAQTLAVTENGQVWSWGDGDYGKLGRGGSD-GCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKP-----EPTALP 757          

HSP 7 Score: 102.834 bits (255), Expect = 6.950e-20
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSS------VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            VY  G G  G+L      ++P   PK   +F G  +      + CG   + A+   G LY  G G+YG+LG G+S+D     P++                    TL ++ N   V+SWGDG+ GKLG G ++ C+ PK+I  L       V  G+  S  LT  G+++++G GD  RLGHG  +  + P+ +  L+G  V  VA G  H
Sbjct:  517 VYSWGCGDGGRLGH--GDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSED--EAIPMLVAGLKGLKVIDVACGSGDAQTLAVTEN-GQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTH 721          

HSP 8 Score: 85.8853 bits (211), Expect = 9.294e-15
Identity = 72/259 (27.80%), Postives = 126/259 (48.65%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLG--HGDKKDYNFPKE-ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            +++  + + L+      +Y WG+   G+LG   G K     P E +++L P+  I  G     A+T DG LY  G G+ GRLG         PTL++++ +V     +V+ G +H L +S +G +V+S+G   +G+LG G+      P++I    G+ +  V A    S  ++ +GDL+ WGK  +       +     PK + ++     +     +G    + + ++ +V+SWG   YGK G G  S   K P ++D
Sbjct: 3939 MNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLI-GGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG-EVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRG-GSDGCKVPMKID 4194          

HSP 9 Score: 81.2629 bits (199), Expect = 2.205e-13
Identity = 65/215 (30.23%), Postives = 99/215 (46.05%), Query Frame = 0

Query: 2542 GKSSVYLVGNGQNGQLADLGSS---SIPPKYADSFGGSS-SVICGL--QVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLV----------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
             +  +Y  G G  G+L   GSS   +IP   A   G     V CG     T A+  NG +++ G+G YGKLG G SD     TP ++  L   +++                 V+SWG G++ +LGHG  E  R PK++  L G     VA G++H   LT+  ++ ++G+ D+ +       TK  P  +  L    +  +ACG   +   FAW
Sbjct:  569 AEGELYTWGRGNYGRLGH-GSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDG--CKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQS---FAW 777          

HSP 10 Score: 76.2554 bits (186), Expect = 6.668e-12
Identity = 59/206 (28.64%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  263 DIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNVHFPS------PIIDASLS-------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTL 433
            D+YTWG             D   PK+ + +     I+    +G + T  +  +  V  W D  +  +          P+   SL+       +    F +ALT+   VY+WG G + RLGHG       P+++  L    ++ I+ G  H    T DG +YTWG+   G+LG  DG  + +  P LV  L    V  V+CG+ HTL
Sbjct: 4113 DLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLG--DGTTNAIQRPRLVAALQGKKVNRVACGSAHTL 4316          

HSP 11 Score: 73.559 bits (179), Expect = 4.734e-11
Identity = 68/253 (26.88%), Postives = 121/253 (47.83%), Query Frame = 0

Query:  340 KVYSWGNGSHGRLG--HGDK-KDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGR--LGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            KV+ WG     +LG   G K K  +F + +S+L  ++ ++ G     A+T++G +Y+ GE + GR  LG   G V  +P  +  L +      +V  G +H   ++ DG +V+S+G G++G+LG         P+LI       ++ +   +  S AL+ SG+L+ WG   +       NT+   PK +  +    +  V+ G      + + +   V+SWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2961 KVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALN-VVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVP-IPRQITALSSYVVKKVAVHSGGRHATALTVDG-KVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRG-GSEGCNIPQNIERLNGQGVC 3209          

HSP 12 Score: 65.0846 bits (157), Expect = 1.571e-8
Identity = 64/263 (24.33%), Postives = 109/263 (41.44%), Query Frame = 0

Query:  220 PVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNV--------HFPSPI-----IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDG--IVHFVPTLVKNL---HNVGSVSCGAQHTLIVSKDGMQVWSFG 446
            P+   K +    G+    H +++   + Y            + ++YTWG             D   P +   +  ++ I+    +G + T  +  NG+V  W D  +  +          P  I     +D    +    F +ALT+D +VYSWG G + RLGHG ++   +PK +  L    ++ ++AG  H  A+T D  +++W  GS  +  H D   +    PT +  L   H VG ++CG   +   S      WS G
Sbjct:  535 PLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITA------EGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSW--GSNDQCQHFDTLRVTKPEPTALPGLDSKHIVG-IACGPAQSFAWSS--CSEWSIG 786          

HSP 13 Score: 62.7734 bits (151), Expect = 9.352e-8
Identity = 55/180 (30.56%), Postives = 80/180 (44.44%), Query Frame = 0

Query: 2679 TDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            T + K+F +G  D+ +LG       K P    +L    V  VA G   +K  FA                    G V+ CG +    + +  S+  +  P++I  L + V   +AV  GG H  AL+  G V+ WG    G+LG+ S     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 2957 TIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGG---SKSLFA----------------VTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTS 3117          

BLAST of EMLSAG00000002245 vs. GO
Match: - (symbol:HERC2 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0020037 "heme binding" evidence=IEA] Pfam:PF00632 InterPro:IPR000433 InterPro:IPR000569 InterPro:IPR001199 InterPro:IPR010606 Pfam:PF00173 Pfam:PF00569 Pfam:PF06701 PROSITE:PS01357 PROSITE:PS50135 PROSITE:PS50237 PROSITE:PS50255 PROSITE:PS51416 SMART:SM00119 SMART:SM00291 INTERPRO:IPR000408 Pfam:PF00415 GO:GO:0008270 GO:GO:0020037 Gene3D:2.60.120.260 InterPro:IPR008979 SUPFAM:SSF49785 Gene3D:2.130.10.30 InterPro:IPR009091 SUPFAM:SSF50985 Gene3D:3.10.120.10 SUPFAM:SSF55856 GO:GO:0004842 PRINTS:PR00633 PROSITE:PS00626 PROSITE:PS50012 InterPro:IPR004939 Pfam:PF03256 PROSITE:PS51284 SUPFAM:SSF56204 Gene3D:2.30.30.30 InterPro:IPR021097 InterPro:IPR014722 Pfam:PF11515 GeneTree:ENSGT00740000115443 InterPro:IPR006624 SMART:SM00706 EMBL:DAAA02003804 EMBL:DAAA02003805 Ensembl:ENSBTAT00000063010 ArrayExpress:G3MX12 Uniprot:G3MX12)

HSP 1 Score: 458.373 bits (1178), Expect = 1.589e-127
Identity = 311/1019 (30.52%), Postives = 498/1019 (48.87%), Query Frame = 0

Query: 2416 AFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDV--------NLKDI-----VWTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A +K     +  Q+EYE   +  G QL++S F + L  LA  L L+T+ C     +W W   +  +      L+KR +      L  LF DE+ K +        N+  +     ++  E D  L++W    P DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  I G Q  FA+  +G LYA G G+ G+LG+G ++     TP +   + H  +                    V+SWG+ EDGKLGHGN   C RP++I SL G     VA G +HSA +T  G ++T+G G  GRLGH + + +  P+ + +L+G+ V  VACG        L      ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ P+++  L+   ++ IA G  H +  S+ G V+ WG N  GQLG+ +    + P ++  L GK + +++ G  H+ AW++            +KP      P  +P E+  L  +P  +    L  L  + E+    + ++ L   +D     P        ++  D +R  L          + + +TM      R +GP V + R+             P G+ S+F Q+  ++    P  L LP+R WKVK VGE+ DD GG + ++I+E+C+EL   +  LL  TPNG  + G+N+D YL +    + V    F FLGVL G+A+RT  P+++NLA  VWK +  +  T  DL +VD  +   L Y+  +     E +++ LP   F   S SG+++ L   S+   ++ +N+ EYV+ A+N R  E   QV AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ ++PS  +I W WE++E F+N  + LFL FV GR+RLP    D   R  +++V      P++ LP + TCFF L+LP Y+ K+++  +L YAI  C++ID D Y
Sbjct: 3738 ALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTS------LPRLFLDEVAKKIRELMADGENMDVLHESHSIFRREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGVGGTESVS--TPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGTEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDVACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLG-HGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCSEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKVNRVACGSAHTLAWST------------SKPASAGKLPVQVPMEYNHLQEIPIIALRNRLLLLHHISELFCPCIPMFDLEGSLDETGLGP--------SVGFDTLRGILISQGKEAAFRKVVQATMVRD---RQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMTSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEICEELQNGLTPLLIVTPNGRDESGANRDCYLFSPAARAPVHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMSLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLP---FTVPSASGQDIQL--SSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGVEPSAPLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDY 4719          

HSP 2 Score: 171.014 bits (432), Expect = 1.455e-40
Identity = 114/370 (30.81%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW--------------------------RESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++ V++ G     QL  L  S I  P ++++    +   V  G +  FA+   G +YACGE + G+LGLG S     +   +T L S+ +  V                  FSWG+G+DGKLGH +   C +P++I +L       +A G+SHSA LT  G+++T+G G+ GRLGHG+  T+  P+ +  L G+ V  VACG        L    + F+W                           + V +I  G  FSL LT SG V+  G   +  +  + S+++++ P+ +  L  + +V +AVG  H LA++ +G V+ WG N  GQ GN +    ++P +++ L G+ I +++ G +HS AWT+
Sbjct: 2879 RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLG-HGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTT 3247          

HSP 3 Score: 155.221 bits (391), Expect = 8.753e-36
Identity = 110/308 (35.71%), Postives = 158/308 (51.30%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP------IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-----WNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK-----RLDIYDIDGICAGPTQSYAWTMRKGKGCFHW------PYSNEPDAKTFSQVSDIL 610
            LAL    +VYSWG G  GRLGHGD      PK IS+         ++ I+ G  ++AAIT +G LYTWG G+ GRLGH       +P LV  L    V  V+CG+    TL V+++G QVWS+G G+ G+LG+G +    TPKLI     + + KV   ++FS+AL+  G ++ WGK         T   V  PK +  +    + +V+ G  H + + E+  V+SWG N   +C     +L+V  P+      LD   I GI  GP QS+AW+      C  W      P+  +  + TF Q+  +L
Sbjct:  419 LALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENG-QVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSND--QC-QHFDTLRVTKPEPSALPGLDTKHIVGIACGPAQSFAWS-----SCSEWSIGLRVPFVVDICSMTFEQLDLLL 717          

HSP 4 Score: 149.828 bits (377), Expect = 3.585e-34
Identity = 90/271 (33.21%), Postives = 145/271 (53.51%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ + +VYSWG    G+LGHG++   + P+ I SL    ++ ++AG  H+A +T  G LYTWG+G  GRLGH D      P LV+ L  H V  V+CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I+   G+ + KV   ++FS+AL+ SG ++ WGK  +       +     P+ +  +    +  ++TG  H +   E+  VY+WG N  G+ G G T+  ++ P+    L    ++ +  G   + AW+  K
Sbjct: 3975 LALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGTEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDVACGSGDAQTLCLTDDDT-VWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCSEDGEVYTWGDNDEGQLGDGTTN-AIQRPRLVAALQGKKVNRVACGSAHTLAWSTSK 4243          

HSP 5 Score: 149.443 bits (376), Expect = 4.728e-34
Identity = 93/266 (34.96%), Postives = 144/266 (54.14%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            ALT D KV+SWG G  G+LGH  + + + P+ I +L    I  I+ G  H+AA+T  G LYTWG G  GRLGH D      P +VK L  H V  V+CG++   TL ++ +G+ V+S+G G+ G+LG+G +     P+ I   +G  + ++    +FSLAL+ SG +W WGK  +       +     P+ +  +    I +V+ G  H + + +   VY+WG N +G+ G G T++  K    + L+   I  +  G + S AWT
Sbjct: 2982 ALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGL-VFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWT 3246          

HSP 6 Score: 124.405 bits (311), Expect = 1.990e-26
Identity = 82/265 (30.94%), Postives = 136/265 (51.32%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFA----TFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPPMPTNFNNSSPTKPRHNIGRPNSLP 2880
            V+SWG G+ G+LGHG+T     PK+IS+ SG  A      +A G+++SA +T +G+++T+G G+ GRLGHG+ + +  P  ++ L+G  V  VACG                   G   +L +T +G V+  G+  +  +    S+   ++PK I KL+++  V +  G    +AL+K G V+ WG     +LG+ +    + P ++E L GK ++ ++AG  H  A         W S     +F+    TKP      P++LP
Sbjct:  427 VYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGS------------------GDAQTLAVTENGQVWSWGDGDYGKLGRGGSD-GCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKP-----EPSALP 667          

HSP 7 Score: 103.605 bits (257), Expect = 4.304e-20
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSS------VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            VY  G G  G+L      ++P   PK   +F G  +      + CG   + A+   G LY  G G+YG+LG G+S+D     P++                    TL ++ N   V+SWGDG+ GKLG G ++ C+ PK+I  L       V  G+  S  LT  G+++++G GD  RLGHG  +  + P+ +  L+G  V  VA G  H
Sbjct:  427 VYSWGCGDGGRLGH--GDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSED--EAIPMLVAGLKGLKVIDVACGSGDAQTLAVTEN-GQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTH 631          

HSP 8 Score: 85.1149 bits (209), Expect = 1.364e-14
Identity = 73/259 (28.19%), Postives = 125/259 (48.26%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLG--HGDKKDYNFPKE-ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            +++  + + L+      +Y WG+   G+LG   G K     P E +++L P+  I  G     A+T DG LY  G G+ GRLG         PTL++++ +V     +V+ G +H L +S +G +V+S+G   +G+LG G+      P++I    G  +  V A    S  ++ +GDL+ WGK  +       +     PK + ++     V     +G    + + ++ +V+SWG   YGK G G  S   K P ++D
Sbjct: 3860 MNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPVQLI-GGEQTLFAVTADGKLYATGYGAGGRLGVGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG-EVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGTEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDVACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRG-GSDGCKVPMKID 4115          

HSP 9 Score: 81.2629 bits (199), Expect = 1.991e-13
Identity = 66/215 (30.70%), Postives = 99/215 (46.05%), Query Frame = 0

Query: 2542 GKSSVYLVGNGQNGQLADLGSS---SIPPKYADSFGGSS-SVICGL--QVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLV----------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
             +  +Y  G G  G+L   GSS   +IP   A   G     V CG     T A+  NG +++ G+G YGKLG G SD     TP ++  L   ++V                 V+SWG G++ +LGHG  E  R PK++  L G     VA G++H   LT+  ++ ++G+ D+ +       TK  P  +  L    +  +ACG   +   FAW
Sbjct:  479 AEGELYTWGRGNYGRLGH-GSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDG--CKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKPEPSALPGLDTKHIVGIACGPAQS---FAW 687          

HSP 10 Score: 74.7146 bits (182), Expect = 2.152e-11
Identity = 41/109 (37.61%), Postives = 59/109 (54.13%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTL 433
            F +ALT+   VY+WG G + RLGHG       P+++  L    ++ I+ G  H    + DG +YTWG+   G+LG  DG  + +  P LV  L    V  V+CG+ HTL
Sbjct: 4131 FSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCSEDGEVYTWGDNDEGQLG--DGTTNAIQRPRLVAALQGKKVNRVACGSAHTL 4237          

HSP 11 Score: 73.1738 bits (178), Expect = 5.879e-11
Identity = 69/260 (26.54%), Postives = 121/260 (46.54%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLG--HGDK-KDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGS-------VSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            A T   KV+ WG     +LG   G K K  +F + +S+L  ++ ++ G     A+T++G +Y  GE + GRLG   GI      + + +  + S       V  G +H   ++ DG +V+S+G G++G+LG         P+LI       ++ +   +  S AL+ SG+L+ WG   +       NT+   PK +  +    +  V+ G      + + +   V+SWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2875 AATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALN-VVQVAGGSKSLFAVTVEGKVYACGEATNGRLGL--GISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDG-KVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRG-GSEGCNIPQNIERLNGQGVC 3129          

HSP 12 Score: 68.1662 bits (165), Expect = 1.933e-9
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTL 433
            F LALT+   V++WG G + RLGHG       P+ +  L    I+ ++ G  H  A+T  G +Y WG+   G+ G+    V+  PTLV+ L    +  V+CG+ H++
Sbjct: 3137 FSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSV 3243          

HSP 13 Score: 64.3142 bits (155), Expect = 3.041e-8
Identity = 59/198 (29.80%), Postives = 87/198 (43.94%), Query Frame = 0

Query: 2661 SGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            SG+     A G   +A +  + K+F +G  D+ +LG       K P    +L    V  VA G   +K  FA                    G V+ CG +    + +  S+  +  P++I  L + V   +AV  GG H  AL+  G V+ WG    G+LG+ S     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 2861 SGSLIRKKAAGLESAATI--RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGG---SKSLFA----------------VTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTS 3037          

HSP 14 Score: 63.929 bits (154), Expect = 3.391e-8
Identity = 49/207 (23.67%), Postives = 88/207 (42.51%), Query Frame = 0

Query:  220 PVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNV--------HFPSPI-----IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWG 395
            P+   K +    G+    H +++   + Y            + ++YTWG             D   P +   +  ++ I+    +G + T  +  NG+V  W D  +  +          P  I     +D    +    F +ALT+D +VYSWG G + RLGHG ++   +PK +  L    ++ ++AG  H  A+T D  +++WG
Sbjct:  445 PLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITA------EGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWG 645          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592955442|gb|GAXK01003114.1| (TSA: Calanus finmarchicus comp187524_c4_seq1 transcribed RNA sequence)

HSP 1 Score: 425.246 bits (1092), Expect = 5.894e-118
Identity = 315/1042 (30.23%), Postives = 490/1042 (47.02%), Query Frame = 0

Query: 2417 FIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKDIVWTPEA---------------DSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVI--DSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPI-------------------GSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---FQIL-RVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
             +K     ++ QYEYE   +  G  L++S F + L  LA  L L+++ C     +W W   +  +    + L  R        L   F  E++K +   ++V   EA               D  L+ W      DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  + G Q  FA+   G +YA G G+ G+LG+G +D     TP +   + H  +                    VFSWG+G+DGKLGH N   C RP++I +L G     ++ G +HSA +T  G+++T+G G  GRLGHG+   +  P+ I +L GY V  VACG    +          ++W +                             K+  G  FS+ LT SG VF  G   +  +  + S+ +++ P+K+  L+   ++ IA G  H +A + SG V+ WG N  GQLG+ S    + P ++  L  + I +++ G  H+ AW++             KP      P ++P EF  LL   S      RL LLH  S+       +FP+    S+   P  +         D +R  L          + + +TM      R +GP V + R F I                      S+F+Q+  ++  I  + L LP+R WKVK VGE+ DD GG + ++I+E+C EL +  +NLL  TPNG  + G+++D  LLN    S   M+ F FLG+L G+A+RT  P+++NLA  VWK I     T  D+ ++D  Y   L  +    + D +  +    D  F   S +G+EV L   +   R++ EN+ EYV+ ALN R  E    V +VR+G++ + P P+  L     +E++VCG    PL +L+ +A Y+ ++ +  +I W W ++EEF+   + LFL FV GR+RLP    D   R    QIL +   P++ LP + TCFF L++P Y+ K ++R +L YAI  C++ID D Y   R N   P+G GD
Sbjct: 12338 LLKGLPEMLLRQYEYEDPIVRGGKHLLHSTFFKELVALACDLGLDSLPCCTETYKWSWFRRYCVAARVATALINRTP------LPQPFCLEVRKKI--MEMVAEDEAFCLDHESHAIFKAGHDEQLLLWLHRKAEDWTLSWGGAGAIYGWGHNHRGQLGGVDGAKVKIPTQCEALSVLRPVQLVGGEQTLFAVTAEGKVYATGYGAGGRLGIGGTDSV--CTPTLLESIQHAFIKKVAVNSGGKHCLALSSEGDVFSWGEGDDGKLGHDNKNSCDRPRVIEALRGKDIVDISCGGAHSAAITSTGELYTWGKGRYGRLGHGDSDDQTKPKLIEALLGYRVIDVACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKVESLAGLGAMKVECGSQFSVCLTRSGSVFTWGKGDYHRLG-HGSDDHVRRPRKVAALQGRKIISIATGSLHCVACTDSGEVFTWGDNDEGQLGDGSTNAIQRPRLVASLQNRKINRVACGSAHTLAWST------------NKPVATSKLPVAVPLEF-DLLKEFSPAILRNRLVLLHHFSDVFCPGLAMFPLGFGSSNTEGPCGV---------DKLRTLLVSAAKESAFRKVVQATMVRD---RQHGPVVELNR-FGIKRNRTKNNSGGGPSTSKEDNGKSVFAQMVSKMGGITNESLLLPHRVWKVKFVGESVDDCGGGYSESIAELCDELMQGQINLLIATPNGRDEAGTSRDCMLLNPTLKSPQHMQMFRFLGLLMGIAVRTGSPLSLNLAEPVWKQIAGAALTPNDITELDKDYVPGLMCIR---DMDGDAKAFAAMDMTFSTPSAAGQEVHL--SNRYRRVTSENRAEYVRLALNYRLHEFDQAVASVREGIARVVPVPLFSLFTGFELETMVCGSPDIPLHLLRSVATYKGVESTAPLIAWFWAVMEEFSTAERSLFLRFVWGRTRLPRSIADFRGRDFVLQILDKYNPPDDFLPESYTCFFLLKMPRYSCKAVLREKLKYAIHFCKSIDTDDY--ARVN--LPSGEGD 15325          

HSP 2 Score: 143.665 bits (361), Expect = 8.979e-33
Identity = 106/333 (31.83%), Postives = 160/333 (48.05%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKD-------NHFSNVHFPSPIIDASLSQ---------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-----NTSSTVPKWXXXXXXXXXX----XTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWTMR 584
            I  GS   +++   GKV +C +         H +NV  P  +  ++LSQ               +ALT D +V+SWG G  G+LGH  +     P+ + +L    +  I+ G  H+AAIT  G LYTWG G  GRLGH D +    P  VK L  H V  V+CG++   TL ++ +GM V+S+G G+ G+LG+G +     P  +   +GV + ++    +FSLALS SG +W WGK  +          V K     S         + G  H + + +   VY+WG N +G+ G G T++  K      LD   +  +  G + S  WT +
Sbjct: 9845 IAGGSKSLFLVTHEGKVYACGEGTNGRLGLGHCNNVAVPRQL--SALSQYVVKKVAVHSGGKHAMALTVDGRVFSWGEGDDGKLGHCSRLSVEKPRIVEALRNKRVRDIACGSSHSAAITSSGELYTWGCGEYGRLGHGDNVTQLRPKCVKALVGHRVVQVACGSRDAQTLALTDEGM-VFSWGDGDFGKLGRGGSEGCAVPANVEKLNGVGIIQIECGAQFSLALSKSGGVWTWGKGDYFRLGHGADQHVRKPTIVESLRGKKIIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGGTTVNRKPALVHGLDTVKVSRVACGSSHSICWTTQ 10834          

HSP 3 Score: 143.28 bits (360), Expect = 1.245e-32
Identity = 98/298 (32.89%), Postives = 145/298 (48.66%), Query Frame = 0

Query: 2578 SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            S+  G +  F +   G +YACGEG+ G+LGLG+ ++     P     LS  +V                    VFSWG+G+DGKLGH +     +P+I+ +L       +A G+SHSA +T  G+++T+G G+ GRLGHG+  T+  P+ + +L G+ V  VACG    +                  +L LT  G VF  G+  F  +    S      P  + KL    ++ I  G    LALSKSG VW WG     +LG+ +    ++P I+E L GK I+ ++ G  H  A T
Sbjct: 9842 SIAGGSKSLFLVTHEGKVYACGEGTNGRLGLGHCNNV--AVPRQLSALSQYVVKKVAVHSGGKHAMALTVDGRVFSWGEGDDGKLGHCSRLSVEKPRIVEALRNKRVRDIACGSSHSAAITSSGELYTWGCGEYGRLGHGDNVTQLRPKCVKALVGHRVVQVACGSRDAQ------------------TLALTDEGMVFSWGDGDFGKLGRGGSE-GCAVPANVEKLNGVGIIQIECGAQFSLALSKSGGVWTWGKGDYFRLGHGADQHVRKPTIVESLRGKKIIHVAVGALHCLAVT 10672          

HSP 4 Score: 130.183 bits (326), Expect = 1.273e-28
Identity = 88/271 (32.47%), Postives = 142/271 (52.40%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWXXXXXXXX--XXXTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ +  V+SWG G  G+LGH +K   + P+ I +L    I+ IS G  H+AAIT  G LYTWG+G  GRLGH D      P L++ L  + V  V+CG+    TL ++ D   VWS+G G+ G+LG+G +     P  +    G+   KV   ++FS+ L+ SG ++ WGK  +       +     P+ + ++      +++TG  H +   ++  V++WG N  G+ G G T+  ++ P+    L    I+ +  G   + AW+  K
Sbjct: 13046 LALSSEGDVFSWGEGDDGKLGHDNKNSCDRPRVIEALRGKDIVDISCGGAHSAAITSTGELYTWGKGRYGRLGHGDSDDQTKPKLIEALLGYRVIDVACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKVPMKVESLAGLGAMKVECGSQFSVCLTRSGSVFTWGKGDYHRLGHGSDDHVRRPRKVAALQGRKIISIATGSLHCVACTDSGEVFTWGDNDEGQLGDGSTN-AIQRPRLVASLQNRKINRVACGSAHTLAWSTNK 13852          

HSP 5 Score: 129.028 bits (323), Expect = 2.706e-28
Identity = 97/272 (35.66%), Postives = 139/272 (51.10%), Query Frame = 0

Query:  327 DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWXXXXXXXXXXXTGGF-HLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDID--GICAGPTQSYAWT 582
            D   F L LT+  +V+SWG G  GRLGHGD+  +  P+ I  L    ++ ISAG   +AA+T +G LY+WG+G+ GRLGH        PTLV  L    V  ++CG+    TL V+++G  V+S+G G+ G+LG+G +    TP+LI+  +G+ + KV    +FSLAL  SG ++ WGK                PK +  IS      + G  H + +     ++ WG N   + G  L S  V  P  L   +    GI  G   S AWT
Sbjct: 1652 DGRHF-LCLTDAGEVWSWGLGDGGRLGHGDQVSHENPQLIQGLVGKKVIQISAGSTFSAAVTQEGELYSWGKGNYGRLGHGTTEDCLTPTLVSGLVGQRVTFIACGSGDAQTLAVAENG-SVYSWGDGDYGKLGRGGSDGSKTPRLIDRLNGLGVIKVFCGAQFSLALLKSGQIYTWGKGDNFRLGHGQEEHVRYPKLVEGISGHVVDLSVGLMHTVAVTREGKLFGWGKNDQAQLGDNL-STTVSVPSLLSPSNSGFVGISCGQAHSLAWT 2458          

HSP 6 Score: 111.309 bits (277), Expect = 6.451e-23
Identity = 74/259 (28.57%), Postives = 127/259 (49.03%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSL-FLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE-NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            V+SWG G+ G+LGHG+      P++I  L G     ++ G++ SA +T +G+++++G G+ GRLGHG  +   +P  +S L G  VT++ACG        +      ++W +                           V K+  G  FSL  L SG ++  G      +  +    +++ PK +  +  ++VD++VG  H +A+++ G ++GWG N   QLG+        P ++   S  G + IS G+ HS AWT+
Sbjct: 1691 VWSWGLGDGGRLGHGDQVSHENPQLIQGLVGKKVIQISAGSTFSAAVTQEGELYSWGKGNYGRLGHGTTEDCLTPTLVSGLVGQRVTFIACGSGDAQTLAVAENGSVYSWGDGDYGKLGRGGSDGSKTPRLIDRLNGLGVIKVFCGAQFSLALLKSGQIYTWGKGDNFRLG-HGQEEHVRYPKLVEGISGHVVDLSVGLMHTVAVTREGKLFGWGKNDQAQLGDNLSTTVSVPSLLSP-SNSGFVGISCGQAHSLAWTN 2461          

HSP 7 Score: 89.7373 bits (221), Expect = 1.895e-16
Identity = 64/218 (29.36%), Postives = 104/218 (47.71%), Query Frame = 0

Query: 2579 VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV------------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKIN------FAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSN 2772
            +  G   + A+   G LY+ G+G+YG+LG G ++D  + T V                   TL ++ N   V+SWGDG+ GKLG G ++  + P++I  L+G     V  G   S  L   G+I+T+G GD  RLGHG  +  + P+ +  + G+ V  ++ GL HT         F W ++  +  LG   S  ++   +    NS F G+    ++
Sbjct: 1799 ISAGSTFSAAVTQEGELYSWGKGNYGRLGHGTTEDCLTPTLVSGLVGQRVTFIACGSGDAQTLAVAEN-GSVYSWGDGDYGKLGRGGSDGSKTPRLIDRLNGLGVIKVFCGAQFSLALLKSGQIYTWGKGDNFRLGHGQEEHVRYPKLVEGISGHVVD-LSVGLMHTVAVTREGKLFGWGKN-DQAQLGDNLSTTVSVPSLLSPSNSGFVGISCGQAH 2443          

HSP 8 Score: 89.7373 bits (221), Expect = 2.027e-16
Identity = 73/256 (28.52%), Postives = 113/256 (44.14%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGS-SSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSDD----------------YFSLTPVVTLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGD---VFFCGNSKFSGMDINSSNMNIQS 2777
            +Y  G G+ G+L    + + + PK   +  G     V CG +   T AL   G +++ G+G +GKLG G S+                         +L LS +   V++WG G+  +LGHG  +  R+P I+ SL G     VAVG  H   +TD G+++ +G+ D G+ G+G     + P  +  L    V+ VACG  H+     W     +        LF  S D    +  GN      D+N+ N N+ S
Sbjct: 10211 LYTWGCGEYGRLGHGDNVTQLRPKCVKALVGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCAVPANVEKLNGVGIIQIECGAQFSLALSKS-GGVWTWGKGDYFRLGHGADQHVRKPTIVESLRGKKIIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGGTTVNRKPALVHGLDTVKVSRVACGSSHS---ICWTTQDNQTSNVHEPVLFANSKDPLGTYLVGNK-----DLNNDNSNVNS 10951          

HSP 9 Score: 85.8853 bits (211), Expect = 3.324e-15
Identity = 56/165 (33.94%), Postives = 84/165 (50.91%), Query Frame = 0

Query: 2586 TFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLVXVF----------------SWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
            T A+  NGS+Y+ G+G YGKLG G SD   S TP ++  L    ++ VF                +WG G++ +LGHG  E  R PK++  +SG+     +VG  H+  +T +GK+F +G  D+ +LG     T   P  +S     FV  ++CG  H+    AW
Sbjct: 1982 TLAVAENGSVYSWGDGDYGKLGRGGSDG--SKTPRLIDRLNGLGVIKVFCGAQFSLALLKSGQIYTWGKGDNFRLGHGQEEHVRYPKLVEGISGHVVDL-SVGLMHTVAVTREGKLFGWGKNDQAQLGDNLSTTVSVPSLLSPSNSGFVG-ISCGQAHS---LAW 2455          

HSP 10 Score: 71.633 bits (174), Expect = 5.509e-11
Identity = 67/238 (28.15%), Postives = 97/238 (40.76%), Query Frame = 0

Query:  329 ESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSSTVPKWXXXXXXXXXXXTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            E  ILA++   ++YS      G L      D N   E  + P  +       H   +T  G +++WG G  GRLGH D + H  P L++ L    V  +S G+  +  V+++G +++S+G GN G LG G T    TP L++   G          R +     SGD                                   + + EN SVYSWG   YGK G G  S   K P+ +D
Sbjct: 1514 ERCILAVSIKARIYSMTYSGDGGL---ITLDTNAMIEKETNPQSVVACPDGRHFLCLTDAGEVWSWGLGDGGRLGHGDQVSHENPQLIQGLVGKKVIQISAGSTFSAAVTQEG-ELYSWGKGNYGRLGHGTTEDCLTPTLVSGLVG---------QRVTFIACGSGD--------------------------------AQTLAVAENGSVYSWGDGDYGKLGRG-GSDGSKTPRLID 2089          

HSP 11 Score: 70.8626 bits (172), Expect = 1.050e-10
Identity = 41/114 (35.96%), Postives = 63/114 (55.26%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTLIVSKD 438
            F + LT    V++WG G + RLGHG       P+++++L    I+ I+ G  H  A T  G ++TWG+   G+LG  DG  + +  P LV +L N  +  V+CG+ HTL  S +
Sbjct: 13514 FSVCLTRSGSVFTWGKGDYHRLGHGSDDHVRRPRKVAALQGRKIISIATGSLHCVACTDSGEVFTWGDNDEGQLG--DGSTNAIQRPRLVASLQNRKINRVACGSAHTLAWSTN 13849          

HSP 12 Score: 69.3218 bits (168), Expect = 3.335e-10
Identity = 65/253 (25.69%), Postives = 122/253 (48.22%), Query Frame = 0

Query:  328 NESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWXXXXXXXXXXXT--GGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
             E  + A+T + KVY+ G G+ GRLG G       P  + S+       + +++G  H  A++ +G +++WGEG  G+LGH +      P +++ L   ++  +SCG  H+  ++  G +++++G G  G LG GD+     PKLI    G  +  V   +  +  L ++ D  +W WG   +            VP  + S++ +  +    G    + +  + SV++WG   Y + G G +   V+ P+++
Sbjct: 12869 GEQTLFAVTAEGKVYATGYGAGGRLGIGGTDSVCTPTLLESIQHAFIKKVAVNSGGKHCLALSSEGDVFSWGEGDDGKLGHDNKNSCDRPRVIEALRGKDIVDISCGGAHSAAITSTG-ELYTWGKGRYGRLGHGDSDDQTKPKLIEALLGYRVIDVACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKVESLAGLGAMKVECGSQFSVCLTRSGSVFTWGKGDYHRLGHG-SDDHVRRPRKV 13621          

HSP 13 Score: 67.3958 bits (163), Expect = 1.152e-9
Identity = 52/183 (28.42%), Postives = 90/183 (49.18%), Query Frame = 0

Query: 2674 HSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVG-GD-HVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA 2851
            H   LTD G+++++G GD GRLGHG+  + ++P+ I  L G                    + V +I  G TFS  +T  G+++  G   +  +  + +  +  +P  +  L  + +  IA G GD   LA++++G V+ WG    G+LG       K P +I+ L+G G++++  G   S A
Sbjct: 1661 HFLCLTDAGEVWSWGLGDGGRLGHGDQVSHENPQLIQGLVG--------------------KKVIQISAGSTFSAAVTQEGELYSWGKGNYGRLG-HGTTEDCLTPTLVSGLVGQRVTFIACGSGDAQTLAVAENGSVYSWGDGDYGKLGRGGSDGSKTPRLIDRLNGLGVIKVFCGAQFSLA 2146          

HSP 14 Score: 65.855 bits (159), Expect = 3.450e-9
Identity = 55/204 (26.96%), Postives = 89/204 (43.63%), Query Frame = 0

Query:  263 DIYTWGCDLHS------------PKIWDDV--NDVEKIETGS--SCTYVIHTNGKVSCWKDNHFSNVHFPSP---IIDASLSQDN----------ESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTL 433
            ++YTWGC  +             PK    +  + V ++  GS  + T  +   G V  W D  F  +         + A++ + N            F LAL++   V++WG G + RLGHG  +    P  + SL    I+ ++ G  H  A+T  G +Y WG+   G+ G+    V+  P LV  L    V  V+CG+ H++
Sbjct: 10208 ELYTWGCGEYGRLGHGDNVTQLRPKCVKALVGHRVVQVACGSRDAQTLALTDEGMVFSWGDGDFGKLGRGGSEGCAVPANVEKLNGVGIIQIECGAQFSLALSKSGGVWTWGKGDYFRLGHGADQHVRKPTIVESLRGKKIIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGNGGTTVNRKPALVHGLDTVKVSRVACGSSHSI 10819          

HSP 15 Score: 51.9878 bits (123), Expect = 5.760e-5
Identity = 56/188 (29.79%), Postives = 85/188 (45.21%), Query Frame = 0

Query:  389 GYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVS-CGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWXXXXXXX--XXXXTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            G +Y WG    G+LG  DG    +PT  + L  +  V   G + TL       +V++ G G  G LG G T  + TP L+       +KKV   +  +  LALS  GD++ WG+            S   P+ I ++      ++S GG H   I     +Y+WG   YG+ G G +  + K PK ++
Sbjct: 12746 GAIYGWGHNHRGQLGGVDGAKVKIPTQCEALSVLRPVQLVGGEQTLFAVTAEGKVYATGYGAGGRLGIGGTDSVCTPTLLESIQHAFIKKVAVNSGGKHCLALSSEGDVFSWGEGDDGKLGHDNKNSCDRPRVIEALRGKDIVDISCGGAHSAAITSTGELYTWGKGRYGRLGHGDSDDQTK-PKLIE 13306          

HSP 16 Score: 51.2174 bits (121), Expect = 8.682e-5
Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 0

Query: 2794 GDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            G H L L+ +G VW WG    G+LG+     ++ P +I+ L GK ++QISAG   SAA T
Sbjct: 1655 GRHFLCLTDAGEVWSWGLGDGGRLGHGDQVSHENPQLIQGLVGKKVIQISAGSTFSAAVT 1834          

HSP 17 Score: 50.0618 bits (118), Expect = 1.892e-4
Identity = 50/217 (23.04%), Postives = 98/217 (45.16%), Query Frame = 0

Query:  392 YTWGEGSLGRLGHKDGIVHFVPT---LVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTPWNT-------SSTVPKWXXXXXXXXXX--XTGGFHLIVILENRSVYSWGLNTYGKCGLG--LTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            + WG     +LG   G    +P    ++ +LH + S++ G++   +V+ +G +V++ G G NG LG G    +  P+ ++     ++KKV   +  + ++AL+V G ++ WG+            S   P+ + ++ +      + G  H   I  +  +Y+WG   YG+ G G  +T L+ K  K L  + +  +  G   +    +      F W
Sbjct: 9740 FVWGLNDKDQLGGLKGSKIKLPVFSEVLSSLHPI-SIAGGSKSLFLVTHEG-KVYACGEGTNGRLGLGHCNNVAVPRQLSALSQYVVKKVAVHSGGKHAMALTVDGRVFSWGEGDDGKLGHCSRLSVEKPRIVEALRNKRVRDIACGSSHSAAITSSGELYTWGCGEYGRLGHGDNVTQLRPKCVKALVGHRVVQVACGSRDAQTLALTDEGMVFSW 10384          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592796363|gb|GAXK01158205.1| (TSA: Calanus finmarchicus comp66507_c1_seq4 transcribed RNA sequence)

HSP 1 Score: 124.405 bits (311), Expect = 3.582e-27
Identity = 100/364 (27.47%), Postives = 167/364 (45.88%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFM-GISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKE-MMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            +    +F     QV  + PK++R   +   VK  GE   D GGV  + +  +  E+      L            S +D Y L  + +S ++  H   F F+G + G+A+     I        +K++L    +  D+E VD +   SLH++      +N+   ++ T + +   SF   +V  L        ++ +NK EYVK  +N RF   +  Q LA++KG + L P  +LK    + +E ++ G  +  L   K   R +     +  ++W W I+E ++ +++   L FV+G SR+P   F   Q          F +  VE   N LP A TCF +L LPPYTS+E M ++L  A++
Sbjct: 1966 LSRGEVFEDSYRQVVKMRPKDMR---KRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWML-----ENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVE 3006          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592796364|gb|GAXK01158204.1| (TSA: Calanus finmarchicus comp66507_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 124.405 bits (311), Expect = 3.589e-27
Identity = 100/364 (27.47%), Postives = 167/364 (45.88%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFM-GISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKE-MMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            +    +F     QV  + PK++R   +   VK  GE   D GGV  + +  +  E+      L            S +D Y L  + +S ++  H   F F+G + G+A+     I        +K++L    +  D+E VD +   SLH++      +N+   ++ T + +   SF   +V  L        ++ +NK EYVK  +N RF   +  Q LA++KG + L P  +LK    + +E ++ G  +  L   K   R +     +  ++W W I+E ++ +++   L FV+G SR+P   F   Q          F +  VE   N LP A TCF +L LPPYTS+E M ++L  A++
Sbjct: 1973 LSRGEVFEDSYRQVVKMRPKDMR---KRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWML-----ENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVE 3013          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592796365|gb|GAXK01158203.1| (TSA: Calanus finmarchicus comp66507_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 124.405 bits (311), Expect = 3.599e-27
Identity = 100/364 (27.47%), Postives = 167/364 (45.88%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFM-GISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKE-MMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            +    +F     QV  + PK++R   +   VK  GE   D GGV  + +  +  E+      L            S +D Y L  + +S ++  H   F F+G + G+A+     I        +K++L    +  D+E VD +   SLH++      +N+   ++ T + +   SF   +V  L        ++ +NK EYVK  +N RF   +  Q LA++KG + L P  +LK    + +E ++ G  +  L   K   R +     +  ++W W I+E ++ +++   L FV+G SR+P   F   Q          F +  VE   N LP A TCF +L LPPYTS+E M ++L  A++
Sbjct: 1981 LSRGEVFEDSYRQVVKMRPKDMR---KRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWML-----ENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVE 3021          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592796366|gb|GAXK01158202.1| (TSA: Calanus finmarchicus comp66507_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 124.405 bits (311), Expect = 3.606e-27
Identity = 100/364 (27.47%), Postives = 167/364 (45.88%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFM-GISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKE-MMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            +    +F     QV  + PK++R   +   VK  GE   D GGV  + +  +  E+      L            S +D Y L  + +S ++  H   F F+G + G+A+     I        +K++L    +  D+E VD +   SLH++      +N+   ++ T + +   SF   +V  L        ++ +NK EYVK  +N RF   +  Q LA++KG + L P  +LK    + +E ++ G  +  L   K   R +     +  ++W W I+E ++ +++   L FV+G SR+P   F   Q          F +  VE   N LP A TCF +L LPPYTS+E M ++L  A++
Sbjct: 1988 LSRGEVFEDSYRQVVKMRPKDMR---KRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWML-----ENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVE 3028          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592943872|gb|GAXK01014681.1| (TSA: Calanus finmarchicus comp9879_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 123.25 bits (308), Expect = 7.456e-27
Identity = 103/334 (30.84%), Postives = 165/334 (49.40%), Query Frame = 0

Query: 3013 VKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKV----DMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF----KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            ++  GE   D GGV      E    LSK++ N       G+ +  S  D Y L  + NS V     + +F F+G + G+A+   K +        +K++L      KD+E VD  Y  SL ++  + +P   E +    +   G + S   + L PG ++ R++ ENKDEY++  +  RF    ++ M Q LA   G + L P  ++K+     +E L+CG  S  +   K    Y+ D  P++ VI W W ++  F+N+++   L FV+G SR+P + F      +  Q F I +   P N  P A TCF +L LPPY S + +R++LI AI+
Sbjct: 1725 IEFDGEVGLDYGGV----AREWFYLLSKEMFNPYY----GLFEY-SATDNYTLQINPNSGVCNEDHLLYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKSIELKDMESVDTEYFNSLIWIKEN-DPSELELAFQVDEENFGQTVS---IELRPGGKETRVTDENKDEYIQLVIEWRFINRVRKQMDQFLA---GFNELVPLNLIKIFDEGELELLMCGIGSIDVKDWKGNTVYKGDYHPNHIVIQWYWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGSPTN-YPRAHTCFNRLDLPPYESYQQLRDKLIKAIE 2675          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592918907|gb|GAXK01039468.1| (TSA: Calanus finmarchicus comp718004_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 115.546 bits (288), Expect = 4.401e-25
Identity = 98/377 (25.99%), Postives = 176/377 (46.68%), Query Frame = 0

Query: 2970 NYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKEL-RLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGR-EVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQ------RFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTID 3337
            N G ++ +RR      S++FS     +  +D  E+ RL  R W ++  GE   D GGV  +    +  E+      L   +      +  N +  L N D      +  F F+G + G+A+  ++ +        +K++L  +    D+E VD  Y  SL ++      DN+ +SL  T       F    E  L PG + I+++ ENK EY+   +  RF   +  Q+    +G S + P  ++K      +E L+ G     +   K    Y++  P+++VI+W W ++  F ++++   L F++G SR+P + F          +F+I +   PE+ LP A TCF +L LP Y++   M+ +++ AI++ +  D
Sbjct:  429 NAGLKMRLRR------SAVFSDSYNIMKKLDRNEVKRLRNRLW-IEFEGEVGYDYGGVSREWFEILSVEMFNPYYGLFEYSAVDNYTLQINPNSGLCNQDH-----LSVFKFIGRVAGMAIMHKRLLNGFFIRPFYKMMLGKQINLSDMECVDAEYYNSLLWIQ-----DNDPESLELTFQVEEEVFGEHTETELVPGGKDIQVTEENKAEYINLVIKWRFISRVREQMTHFLQGFSDILPLRMIKTFDEGELEFLMSGIGVIDVKDWKDNTEYKNYRPTDKVILWFWRMVLSFGDEMRSKLLQFITGTSRVPYNGFKELHGSNGPCKFKISKSGSPES-LPRAHTCFNRLDLPEYSTYRNMKKKIVTAIEYSQGFD 1505          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592918909|gb|GAXK01039466.1| (TSA: Calanus finmarchicus comp718004_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 114.775 bits (286), Expect = 8.241e-25
Identity = 102/381 (26.77%), Postives = 176/381 (46.19%), Query Frame = 0

Query: 2970 NYGPQVTVRRLFPIGSS-SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKV-DMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGR-EVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQ------RFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTID 3337
            N G ++ VRR      S SIF ++         +  RL  R W ++  GE   D GGV  +    +  E+      L            S  D Y L  + NS + +  H   F F+G + G+A+  ++ +        +K++L    T  D+E VD  Y  SL ++      +N+ +SLL T       F    E  L PG   I+++ ENK EY+   +  RF   +  Q+    +G+S + P  ++K      +E L+ G     +   K    Y++  P+++VI+W W ++  F ++++   L F++G SR+P + F          +F+I +   PE+ LP A TCF +L LP Y++   M+ +++ AI++ +  D
Sbjct:  525 NNGVKLRVRRSEVFSDSYSIFRKLGRN------EVKRLRNRLW-IEFEGEVGYDYGGVSREWFEILSVEMFNPYYGLFE---------YSAVDNYTLQINPNSGLCNQDHLAVFKFIGRVAGMAIMHKRLLNGFFIRPFYKMMLGKMITISDMESVDAEYYNSLVWIK-----ENDPESLLLTFQVEEEVFGEHTETELVPGGGDIQVTEENKMEYIDMVIKWRFVSRIRDQMAHFLQGISDIIPLKMIKTFDEGELEFLMSGIGVIDVKDWKDNTEYKNYRPTDKVILWFWRMVLSFGDEMRSKLLQFITGTSRVPYNGFKELHGSNGPCKFKISKSGSPES-LPRAHTCFNRLDLPEYSTYRNMKKKIVTAIEYSQGFD 1601          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592924985|gb|GAXK01033430.1| (TSA: Calanus finmarchicus comp82126_c3_seq2 transcribed RNA sequence)

HSP 1 Score: 115.161 bits (287), Expect = 3.408e-24
Identity = 104/413 (25.18%), Postives = 183/413 (44.31%), Query Frame = 0

Query: 2954 LMQTLMSTMTNSNVLRN---YGPQVTVRRLFPIGSS------SIFSQIAEQVFYIDPKELRL-------PYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIK-TTWKDLEDVDLS---YSRSLHYVATSFNPDNEEDSLLPTDYFMGISF---SGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKE--MMPQVLAVRKGLSALAPYPILK-LLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR-----FQILRVERPE----NGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAI 3330
            L   +++   N++  RN     P+++V  +  IG++      S+F Q   Q+  I   EL +       P   + +K+VG+      G F   +S +  +L    LNLL P     S  G+   ++ L     +  + +   F G L G++LR+  P+ +NL P  W+ ++ +    W +   +D     Y  +L  V        + DS L  + F   ++   +G  V L PG   + L++EN+ EYVK+    R KE     +   +  GL  +AP   ++ +  ++ IE   CG+ +  L  LK    Y+  +   ++ ++  W +LE F  +    F+ F   + R+P    + S        +I   +R E          +TC F ++LP YT+ E MR +L+YAI
Sbjct: 2876 LKSGMLTKYLNASHQRNPDHAPPEISVDPVEEIGNTNHDVCLSLFCQALNQLSEIQSNELNVSIAFGGDPQFPFNIKMVGDEVHGNSGSFRHFLSTIVDQLHSPTLNLLVP----YSGTGAFMGRHFLKPGPLNYGEEKMLQFFGQLLGISLRSGIPLPLNLMPTFWRALVNLPCQAWPECSAIDPVTNLYLSNLESVTKE-----KFDSFLDENDFPKFTYHSLNGEVVELCPGGVNMPLTWENRSEYVKSIKEHRIKEWGSRDRFSHIVAGLGTIAPVSFIQNIFTAEDIELKFCGQPNVDLKFLKCHTIYQVGITSEDQHVLQFWAVLESFTQNELAKFIKFACNQDRIPMVGPEESSHIPPYPMKIAPPDRREGEQDQQFIRVETCMFMIKLPRYTTYETMREKLLYAI 4087          

BLAST of EMLSAG00000002245 vs. C. finmarchicus
Match: gi|592924986|gb|GAXK01033429.1| (TSA: Calanus finmarchicus comp82126_c3_seq1 transcribed RNA sequence)

HSP 1 Score: 115.161 bits (287), Expect = 4.632e-24
Identity = 104/413 (25.18%), Postives = 183/413 (44.31%), Query Frame = 0

Query: 2954 LMQTLMSTMTNSNVLRN---YGPQVTVRRLFPIGSS------SIFSQIAEQVFYIDPKELRL-------PYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIK-TTWKDLEDVDLS---YSRSLHYVATSFNPDNEEDSLLPTDYFMGISF---SGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKE--MMPQVLAVRKGLSALAPYPILK-LLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR-----FQILRVERPE----NGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAI 3330
            L   +++   N++  RN     P+++V  +  IG++      S+F Q   Q+  I   EL +       P   + +K+VG+      G F   +S +  +L    LNLL P     S  G+   ++ L     +  + +   F G L G++LR+  P+ +NL P  W+ ++ +    W +   +D     Y  +L  V        + DS L  + F   ++   +G  V L PG   + L++EN+ EYVK+    R KE     +   +  GL  +AP   ++ +  ++ IE   CG+ +  L  LK    Y+  +   ++ ++  W +LE F  +    F+ F   + R+P    + S        +I   +R E          +TC F ++LP YT+ E MR +L+YAI
Sbjct: 11757 LKSGMLTKYLNASHQRNPDHAPPEISVDPVEEIGNTNHDVCLSLFCQALNQLSEIQSNELNVSIAFGGDPQFPFNIKMVGDEVHGNSGSFRHFLSTIVDQLHSPTLNLLVP----YSGTGAFMGRHFLKPGPLNYGEEKMLQFFGQLLGISLRSGIPLPLNLMPTFWRALVNLPCQAWPECSAIDPVTNLYLSNLESVTKE-----KFDSFLDENDFPKFTYHSLNGEVVELCPGGVNMPLTWENRSEYVKSIKEHRIKEWGSRDRFSHIVAGLGTIAPVSFIQNIFTAEDIELKFCGQPNVDLKFLKCHTIYQVGITSEDQHVLQFWAVLESFTQNELAKFIKFACNQDRIPMVGPEESSHIPPYPMKIAPPDRREGEQDQQFIRVETCMFMIKLPRYTTYETMREKLLYAI 12968          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000002245 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12")

HSP 1 Score: 6895.44 bits (17889), Expect = 0.000e+0
Identity = 3360/3360 (100.00%), Postives = 3360/3360 (100.00%), Query Frame = 0

Query:    1 MSKKEEEVCRWKCLGFAAFGDVDFQLQTENLFRLASAQAQQIRTSSYFQNRLIFKLQGLENKECAEVENKDDILKLDLQRSESFSNPEEDTCRSESLQNEALLNLGLQTGLSLLFSLLQQNWSGPDEQHPLSSTVISTANNMLLSFPPLSLLAVPPLGETALDNVATFLHTSAQIPQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGCDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFSNVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGKDAAKKLLQHLVYKMHHDFVQSLFECDSKMFDLEMSVGYSIHWQYLISQKSSNEFKDLRQYIIEVLKLGECLIESFIKKVKVDSTFIISHTKPPHKFTSVFESTLLFLINNPQYTPLSIKKLRETSLKLNCILKDENSFLMDIHVLISILSGHIIGSKISFFKPQECSVLYXCSQTFFKYVILIPHVYKSRCSISNCFEEMKNVVCCDIYTLNSETLGTLVSLARSHFTGNQLESILTSFNLDYIQSVQTLLLDLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIENFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLLECLTGCPSILELSIVRCLGKILSHFEIGRNREHIVRKLFSHIYNSYWNSKSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINNRSIKDMWLYMSSGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKAKISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKLRGSIDNVLEAIYPLLALLSDVDSGLCLGSYVRYKEFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSLASSSEFESANSSSNVSSCYFDIRSYFTSRRSSLDDTMINIASNSVPVRRTFRSDILKVNPNIYLMEHLKDIYDYDCLNVHDKEKINVEDLRWLGPINVKADPLGQQDLKNGLRDLNTSSMVDISEEINNRMYLQYSNFLSLNMKLEEMFYSLEYLKKIIQIKCTQDLLLAYFKNVKHIPIPEIINISFCYPFLFEFARMMGHTDPMEGLYFKNEKELCCSFGPDIEVLKVILYEAKNKLPSFLALCNICVINLMNLSKDKHTXTVNEKXAIYITQILTEIVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNEMDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGDAGHN 3360
            MSKKEEEVCRWKCLGFAAFGDVDFQLQTENLFRLASAQAQQIRTSSYFQNRLIFKLQGLENKECAEVENKDDILKLDLQRSESFSNPEEDTCRSESLQNEALLNLGLQTGLSLLFSLLQQNWSGPDEQHPLSSTVISTANNMLLSFPPLSLLAVPPLGETALDNVATFLHTSAQIPQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGCDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFSNVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGKDAAKKLLQHLVYKMHHDFVQSLFECDSKMFDLEMSVGYSIHWQYLISQKSSNEFKDLRQYIIEVLKLGECLIESFIKKVKVDSTFIISHTKPPHKFTSVFESTLLFLINNPQYTPLSIKKLRETSLKLNCILKDENSFLMDIHVLISILSGHIIGSKISFFKPQECSVLYXCSQTFFKYVILIPHVYKSRCSISNCFEEMKNVVCCDIYTLNSETLGTLVSLARSHFTGNQLESILTSFNLDYIQSVQTLLLDLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIENFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLLECLTGCPSILELSIVRCLGKILSHFEIGRNREHIVRKLFSHIYNSYWNSKSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINNRSIKDMWLYMSSGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKAKISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKLRGSIDNVLEAIYPLLALLSDVDSGLCLGSYVRYKEFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSLASSSEFESANSSSNVSSCYFDIRSYFTSRRSSLDDTMINIASNSVPVRRTFRSDILKVNPNIYLMEHLKDIYDYDCLNVHDKEKINVEDLRWLGPINVKADPLGQQDLKNGLRDLNTSSMVDISEEINNRMYLQYSNFLSLNMKLEEMFYSLEYLKKIIQIKCTQDLLLAYFKNVKHIPIPEIINISFCYPFLFEFARMMGHTDPMEGLYFKNEKELCCSFGPDIEVLKVILYEAKNKLPSFLALCNICVINLMNLSKDKHTXTVNEKXAIYITQILTEIVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNEMDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGDAGHN
Sbjct:    1 MSKKEEEVCRWKCLGFAAFGDVDFQLQTENLFRLASAQAQQIRTSSYFQNRLIFKLQGLENKECAEVENKDDILKLDLQRSESFSNPEEDTCRSESLQNEALLNLGLQTGLSLLFSLLQQNWSGPDEQHPLSSTVISTANNMLLSFPPLSLLAVPPLGETALDNVATFLHTSAQIPQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGCDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFSNVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGKDAAKKLLQHLVYKMHHDFVQSLFECDSKMFDLEMSVGYSIHWQYLISQKSSNEFKDLRQYIIEVLKLGECLIESFIKKVKVDSTFIISHTKPPHKFTSVFESTLLFLINNPQYTPLSIKKLRETSLKLNCILKDENSFLMDIHVLISILSGHIIGSKISFFKPQECSVLYXCSQTFFKYVILIPHVYKSRCSISNCFEEMKNVVCCDIYTLNSETLGTLVSLARSHFTGNQLESILTSFNLDYIQSVQTLLLDLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIENFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLLECLTGCPSILELSIVRCLGKILSHFEIGRNREHIVRKLFSHIYNSYWNSKSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINNRSIKDMWLYMSSGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKAKISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKLRGSIDNVLEAIYPLLALLSDVDSGLCLGSYVRYKEFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSLASSSEFESANSSSNVSSCYFDIRSYFTSRRSSLDDTMINIASNSVPVRRTFRSDILKVNPNIYLMEHLKDIYDYDCLNVHDKEKINVEDLRWLGPINVKADPLGQQDLKNGLRDLNTSSMVDISEEINNRMYLQYSNFLSLNMKLEEMFYSLEYLKKIIQIKCTQDLLLAYFKNVKHIPIPEIINISFCYPFLFEFARMMGHTDPMEGLYFKNEKELCCSFGPDIEVLKVILYEAKNKLPSFLALCNICVINLMNLSKDKHTXTVNEKXAIYITQILTEIVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNEMDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGDAGHN 3360          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000012949 (pep:novel supercontig:LSalAtl2s:LSalAtl2s98:1049432:1083512:-1 gene:EMLSAG00000012949 transcript:EMLSAT00000012949 description:"maker-LSalAtl2s98-augustus-gene-10.16")

HSP 1 Score: 364.385 bits (934), Expect = 1.249e-100
Identity = 279/947 (29.46%), Postives = 440/947 (46.46%), Query Frame = 0

Query: 2403 EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDV-------------NLKDIVWTPEADSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGE-----------GSYGKLGLGNSDDYFSLTPVVT-------LLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGL-EHTKINFAWRESVTKICLGRTFSLFLTSGD---VFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPV----IDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL-----------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV 3294
            +H PF          +K     ++ Q+EYE   +  G  L++S F + L  LA  ++L+ + C     +W W   +  +      L  R       +L  LF +++++ +             +    ++T E D  L+ W    P DW    GG  ++Y  G+   GQL                GG+     G Q  FA+  +G +YA G             S  +  L  S  + ++  V         L LS +   V+SWG+G+DGKLGHG+   C RP++I +L G     ++ G +HS+ +T  G+++T+G G  GRLGHG+ + +  P+ I  L  + V  VACG  +   +     ++V     G    L     D   VF  G   +  +   + + +++ PKK+  L++  ++ IA G  H +A +  G V+ WG N  GQLG+ S    + P ++  L GK I +++ G  H+ A +     TN + ++ T P H       +P E+  LL   S      RL LLH  S+       +FP+         +    I+D   + S D +R  L          + + +TM      R +GP V + R+            P GS S+FSQ+      +  K L LP+R WKVK +GE+ DD GG + ++I+EMC EL    L+LL  TPNG  + G+++D +LLN   NS +    F FLG+L G+A+RT  P+++NLA  +WKL+        D+ +VD  Y   L  +    +PD +  S L  D  F   S +G +V L   +   R++ EN++EYVK A+  R  E    V A+R+G+S + P P+L L     +E +VCG                 +D S  ++ W WE++EEF+   + LFL FV GR+RLP    D   R  +L+V
Sbjct: 3843 DHQPFYTDTA-LSVLVKGLPKMLLRQFEYEDEIVRGGKHLLHSNFFKELVALACDISLDKLSCYSETYKWAWFQRYCMASRVAKALINR------AILPKLFCNDVRRKIEEMSSEHEEYTSDHENHQIFTQEHDRQLLLWLHRKPEDWTLSWGGAGTIYGWGHNHRGQL----------------GGADG---GEQTLFAVTADGKVYATGYGAGGRLGIGGVDSVSEPTLLESIQHINIKTVSVNSGGKHCLALSAD-GEVYSWGEGQDGKLGHGSRTSCDRPRVIEALRGKDVIAISCGGAHSSAITANGELYTWGRGRYGRLGHGDVEDQLKPKLIEYLIDHRVIDVACGSGDAQTLCITDDDNVWSFGDGDYGKLGRGGSDGWSVFTWGKGDYHRLGHGTED-HVRRPKKVASLQSKKVISIATGSLHCVACTDQGEVYTWGDNDEGQLGDGSTNAIQRPRLVASLQGKKINKVACGSAHTLAXS-----TNKSIATSTLPSH-------VPLEY-DLLNGLSPMLLRNRLVLLHSFSDIFCPGLAMFPLGLVGKSEKEHHQQFIRD--DSFSIDKLRTILVTSSKESAFRKVIQATMVRD---RQHGPIVELIRISCGNKKPKTKGAPDGSQSVFSQMTSNTSLLGDKCLLLPHRVWKVKFIGESVDDCGGGYSESIAEMCDELQNGTLSLLISTPNGRDEAGTSRDCFLLNPALNSSLYKNMFRFLGILMGIAVRTGSPLSLNLAEPMWKLLAGACLNLNDITEVDKDYVPGLLCI-RDMDPDPK--SFLDMDMPFSTPSCAGHDVQL--STRYSRVTLENRNEYVKLAIQYRLNEFDFAVSAIREGMSRVIPVPLLSLFTGYELEVMVCGR----------------IDSSAPLVEWFWEVMEEFSTSERSLFLRFVWGRTRLPRSIADFRGRDFVLQV 4722          

HSP 2 Score: 160.999 bits (406), Expect = 4.403e-39
Identity = 114/377 (30.24%), Postives = 182/377 (48.28%), Query Frame = 0

Query: 2544 SSVYLVGNGQNGQLADLGSSSIP-PKYADSFGG--SSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSP-PMPTN 2860
            + V++ G     QL  L  S I  P Y++       + +  G +  F +  +G +YACGEG+ G+LGL NS +     P     LS  +V                    VFSWG+G+DGKLGH +     +P++I +L       +A G+SHSA +T  G+++T+G G+ GRLGHG+  T+  P+Q+ +L G  V  VACG        L    + ++W +                           V +I  G  FSL L+ +G V+  G   +  +  + ++ +I+ P  +  L  + +V +AVG  H LA++  G V+ WG N  GQ GN +    ++P ++  L G  I +++ G +HS  WT+P  MP+N
Sbjct: 2989 TKVFVWGLNDKDQLGGLKGSKIKLPVYSEILSNLKPTCIAGGSKSLFVVTQDGKVYACGEGTNGRLGLSNSCNV--TIPKQMTALSQYVVKKVAVHSGGKHAMALTVDGRVFSWGEGDDGKLGHCSRAPIPKPRLIEALKSKRVRDIACGSSHSAAITSNGELYTWGCGEXGRLGHGDNITQMRPKQVKTLAGKRVIQVACGSRDAQTLALTDDGMVYSWGDGDFGKLGRGGSEGCNKPQNVEKLNGVGVCQIECGAQFSLALSRNGLVWTWGKGDYFRLG-HGADQHIRKPSVVECLRGKKIVHVAVGALHCLAVTDLGQVFAWGDNDHGQQGNATTTVNRKPDLVNGLDGHRISRVACGSSHSVCWTTPIIMPSN 3362          

HSP 3 Score: 148.288 bits (373), Expect = 2.797e-35
Identity = 106/331 (32.02%), Postives = 163/331 (49.24%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKDNHFS-------NVHFPSPIIDASLSQ---------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            I  GS   +V+  +GKV +C +  +         NV  P  +   +LSQ               +ALT D +V+SWG G  G+LGH  +     P+ I +L    +  I+ G  H+AAIT +G LYTWG G  GRLGH D I    P  VK L    V  V+CG++   TL ++ DGM V+S+G G+ G+LG+G +     P+ +   +GV + ++    +FSLALS +G +W WGK  +       +     P  +  +    I +V+ G  H + + +   V++WG N +G+ G   T++  K      LD + I  +  G + S  WT
Sbjct: 3027 IAGGSKSLFVVTQDGKVYACGEGTNGRLGLSNSCNVTIPKQM--TALSQYVVKKVAVHSGGKHAMALTVDGRVFSWGEGDDGKLGHCSRAPIPKPRLIEALKSKRVRDIACGSSHSAAITSNGELYTWGCGEXGRLGHGDNITQMRPKQVKTLAGKRVIQVACGSRDAQTLALTDDGM-VYSWGDGDFGKLGRGGSEGCNKPQNVEKLNGVGVCQIECGAQFSLALSRNGLVWTWGKGDYFRLGHGADQHIRKPSVVECLRGKKIVHVAVGALHCLAVTDLGQVFAWGDNDHGQQGNATTTVNRKPDLVNGLDGHRISRVACGSSHSVCWT 3354          

HSP 4 Score: 123.635 bits (309), Expect = 8.545e-28
Identity = 88/271 (32.47%), Postives = 132/271 (48.71%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-----TSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ D +VYSWG G  G+LGHG +   + P+ I +L    ++ IS G  H++AIT +G LYTWG G  GRLGH D      P L++ L  H V  V+CG+    TL ++ D   VWSFG G+ G+LG+G +            DG                     ++ WGK  ++     T   V  PK + S+ S  + +++TG  H +   +   VY+WG N  G+ G G T+  ++ P+    L    I+ +  G   + A +  K
Sbjct: 4070 LALSADGEVYSWGEGQDGKLGHGSRTSCDRPRVIEALRGKDVIAISCGGAHSSAITANGELYTWGRGRYGRLGHGDVEDQLKPKLIEYLIDHRVIDVACGSGDAQTLCITDDD-NVWSFGDGDYGKLGRGGS------------DG-------------------WSVFTWGKGDYHRLGHGTEDHVRRPKKVASLQSKKVISIATGSLHCVACTDQGEVYTWGDNDEGQLGDGSTN-AIQRPRLVASLQGKKINKVACGSAHTLAXSTNK 4307          

HSP 5 Score: 85.1149 bits (209), Expect = 4.320e-16
Identity = 68/251 (27.09%), Postives = 117/251 (46.61%), Query Frame = 0

Query:  340 KVYSWGNGSHGRLG--HGDKKDYNFPKEI-SSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            KV+ WG     +LG   G K       EI S+L P  CI+ G      +T DG +Y  GEG+ GRLG  +     +P  +  L        +V  G +H + ++ DG +V+S+G G++G+LG      +  P+LI       ++ +   +  S A++ +G+L+ WG           N +   PK + +++   +  V+ G      + + ++  VYSWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2990 KVFVWGLNDKDQLGGLKGSKIKLPVYSEILSNLKP-TCIAGGSKSLFVVTQDGKVYACGEGTNGRLGLSNSCNVTIPKQMTALSQYVVKKVAVHSGGKHAMALTVDG-RVFSWGEGDDGKLGHCSRAPIPKPRLIEALKSKRVRDIACGSSHSAAITSNGELYTWGCGEXGRLGHGDNITQMRPKQVKTLAGKRVIQVACGSRDAQTLALTDDGMVYSWGDGDFGKLGRG-GSEGCNKPQNVEKLNGVGVC 3237          

HSP 6 Score: 82.4185 bits (202), Expect = 2.426e-15
Identity = 62/220 (28.18%), Postives = 99/220 (45.00%), Query Frame = 0

Query: 2628 NLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAG 2845
            N   VF WG  +  +LG     + + P     LS    T +A G+    V+T  GK++  G G  GRLG  N      P+Q+++L  Y V  VA       ++   + ++     GR FS     GD          G   + S   I  P+ I  L++  + DIA G  H  A++ +G ++ WGC   G+LG+        P  ++ L+GK ++Q++ G
Sbjct: 2987 NSTKVFVWGLNDKDQLGGLKGSKIKLPVYSEILSNLKPTCIAGGSKSLFVVTQDGKVYACGEGTNGRLGLSNSCNVTIPKQMTALSQYVVKKVA-------VHSGGKHAMALTVDGRVFS--WGEGD---------DGKLGHCSRAPIPKPRLIEALKSKRVRDIACGSSHSAAITSNGELYTWGCGEXGRLGHGDNITQMRPKQVKTLAGKRVIQVACG 3188          

HSP 7 Score: 70.0922 bits (170), Expect = 1.266e-11
Identity = 64/243 (26.34%), Postives = 109/243 (44.86%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVG----SVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNT-------SSTVPKWINSI--SSISNVS--TGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            L +  E + L+      +Y WG+   G+LG  D                     G     A+T DG +Y  G G+ GRLG         PTL++++ ++     SV+ G +H L +S DG +V+S+G G +G+LG G       P++I    G  +  +      S A++ +G+L+ WG+  +             PK I  +    + +V+  +G    + I ++ +V+S+G   YGK G G
Sbjct: 3977 LHRKPEDWTLSWGGAGTIYGWGHNHRGQLGGAD--------------------GGEQTLFAVTADGKVYATGYGAGGRLGIGGVDSVSEPTLLESIQHINIKTVSVNSGGKHCLALSADG-EVYSWGEGQDGKLGHGSRTSCDRPRVIEALRGKDVIAISCGGAHSSAITANGELYTWGRGRYGRLGHGDVEDQLKPKLIEYLIDHRVIDVACGSGDAQTLCITDDDNVWSFGDGDYGKLGRG 4198          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000006110 (pep:novel supercontig:LSalAtl2s:LSalAtl2s332:214808:219258:-1 gene:EMLSAG00000006110 transcript:EMLSAT00000006110 description:"maker-LSalAtl2s332-snap-gene-2.14")

HSP 1 Score: 115.931 bits (289), Expect = 1.023e-25
Identity = 75/229 (32.75%), Postives = 116/229 (50.66%), Query Frame = 0

Query:  341 VYSWGNGSHGRLGHGDKKDYN--FPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNT----SSTVPKWINSISSISNV------------STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            +YSWG G+HG+LG G  ++ N   P  ++    I  ISAG  H A +T +G +YT G    G+LGH      F    + N +++ SV CG QHTL + + G +V+S+G    G+ G  D G   TPK+I       + ++ A +  SLAL+  G ++ WG   +        +  K +     +SNV            + G  H +++  + +VYSWG N+YG+ GLG
Sbjct:    1 MYSWGLGNHGQLGLGAIEETNVGIPSLVNLDEDIDVISAGSEHTAVLTTEGKIYTCGSNDYGQLGHSSKQSRFEKVELLNSYSIKSVVCGGQHTLALDEWG-KVFSWGSDTRGQTGH-DDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGTVYSWGKNSYGQLGLG 227          

HSP 2 Score: 108.612 bits (270), Expect = 1.648e-23
Identity = 89/332 (26.81%), Postives = 161/332 (48.49%), Query Frame = 0

Query:  264 IYTWGCDLHS--------------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFSNVHFPSP-----IIDASLSQDNESFI------LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISS--LPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLG----HKDGIV---HFVPTLVKNLHNVGS--VSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSI--SSISNVSTGGFHLIVILENRS-VYSWGLNTYGKCG 549
            +Y+WG   H               P + +   D++ I  GS  T V+ T GK+     N +  +   S       ++   S   +S +      LAL E  KV+SWG+ + G+ GH D  + + PK I S  L  +  I+AGH+H+ A+T  G +Y WG+ + G+LG      D +V     +  ++++L  V    ++ GA+H +IV+  G  V+S+G  + G+LG GD    Y P+ I     +I+K +   N  S+ L+  G ++ +G+  +         +  +P+ +  +  +++S ++ G  H + ++ +R  +YS+GL   G+ G
Sbjct:    1 MYSWGLGNHGQLGLGAIEETNVGIPSLVNLDEDIDVISAGSEHTAVLTTEGKIYTCGSNDYGQLGHSSKQSRFEKVELLNSYSIKSVVCGGQHTLALDEWGKVFSWGSDTRGQTGH-DDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGT-VYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGHGSFNNELLPRKVMELMGTAVSQIACGARHTLALIPSRERLYSFGLGGSGQLG 330          

HSP 3 Score: 103.99 bits (258), Expect = 4.081e-22
Identity = 71/227 (31.28%), Postives = 104/227 (45.81%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIPPKYADSFG--GSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGN-------------SDDYFSLTPVVTLLLS----HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSL 2748
            V+  G+   GQ      +   PK   S G    S +  G   + AL   G +YA G+ +YG+LGLG              S+   SL  V  L+++    H ++      V+SWG    G+LG G+      P+ I  L      F++ G  HS VLT+ G +F+FG G  G+LGHG+F  +  P ++  L G  V+ +ACG  HT      RE +    LG +  L
Sbjct:  103 VFSWGSDTRGQTGHDDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGTVYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGHGSFNNELLPRKVMELMGTAVSQIACGARHTLALIPSRERLYSFGLGGSGQL 329          

HSP 4 Score: 101.679 bits (252), Expect = 2.446e-21
Identity = 93/296 (31.42%), Postives = 132/296 (44.59%), Query Frame = 0

Query: 2581 CGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYF---------SLTPVV-----TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKK--SPEQ-----ISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVD--IAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKE--PIIIEELSGKGILQISAGKNHSAA 2851
             G + T  L   G +Y CG   YG+LG  +    F         S+  VV     TL L      VFSWG    G+ GH +  +   PKII SL     + +A G  HS  LT+KGKI+ +G+   G+LG G     K   P++     I SL G     +A G EH  I       VT             SG V+  G + +  + +   N N   P++I  L +++   I+ G DH + L++ G V+ +G    GQLG+  G F  E  P  + EL G  + QI+ G  H+ A
Sbjct:   39 AGSEHTAVLTTEGKIYTCGSNDYGQLGHSSKQSRFEKVELLNSYSIKSVVCGGQHTLALDE-WGKVFSWGSDTRGQTGHDDGNK-DTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMI-------VT------------ISGTVYSWGKNSYGQLGLGDVN-NSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGH--GSFNNELLPRKVMELMGTAVSQIACGARHTLA 310          

HSP 5 Score: 100.138 bits (248), Expect = 6.487e-21
Identity = 69/241 (28.63%), Postives = 111/241 (46.06%), Query Frame = 0

Query:  264 IYTWGCDLHSPKIWDDVN-------------DVEKIETGSSCTYVIHTNGKVSCWKDNHFSNVHFPSPIIDA------SLSQDNESFI------LALTEDH--------KVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMC--ISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLI 467
            +++WG D       DD N             +V +I  G   +  +   GK+  W DN +  +       D        LS   ES I      +A   +H         VYSWG  S+G+LG GD  +  FP++I  L  ++   IS G+ H+  +T DG ++++G+G  G+LGH       +P  V  L    V  ++CGA+HTL +     +++SFG G +G+LG+     +  P++I
Sbjct:  103 VFSWGSDTRGQTGHDDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGTVYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGHGSFNNELLPRKVMELMGTAVSQIACGARHTLALIPSRERLYSFGLGGSGQLGREAXENVNIPQII 343          

HSP 6 Score: 93.2041 bits (230), Expect = 8.284e-19
Identity = 87/307 (28.34%), Postives = 134/307 (43.65%), Query Frame = 0

Query:  283 VEKIETGSSCTYVIHTNGKVSCW-KDNHFSNVH----FPSPIIDASLSQDNESFI-------LALTEDHKVYSWGNGSHGRLGHGDKKD----------YNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLA------------LSVSGDLW------------IWGKTPWNTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWG 541
            ++ +  G   T  +   GKV  W  D      H      +P I  SL   N S I       LALT   K+Y+WG+ ++G+LG G +             N  + +  + P+M I+AG  H   +T+ G +Y+WG+ S G+LG  D    + P  ++ L +  +  +SCG  H++++++DG  V+SFG G  G+LG G       P+ +    G  + ++    R +LA            L  SG L             I+G  PW   S  PK IN   S S  S        I EN ++Y+ G
Sbjct:   84 IKSVVCGGQHTLALDEWGKVFSWGSDTRGQTGHDDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLM-IAAGAEHXMIVTISGTVYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDG-GVFSFGQGRFGQLGHGSFNNELLPRKVMELMGTAVSQIACGARHTLALIPSRERLYSFGLGGSGQLGREAXENVNIPQIIYG--PWXQPSGQPKRINRQHSSSGSS-------YIEENVALYTIG 379          

HSP 7 Score: 92.8189 bits (229), Expect = 1.041e-18
Identity = 73/226 (32.30%), Postives = 106/226 (46.90%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRR--PKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQ--LGNESGYFYKEPI-----IIEELSGKGILQISAGKNH 2848
            ++SWG G  G+LG G  E      P +++ L  +    ++ G+ H+AVLT +GKI+T G+ D G+LGH + Q++   E++  L  Y +  V CG +HT     W                   G VF  G+        +  N +     K   L+N+  IA G  H LAL+  G ++ WG N  GQ  LG ESG    +P      +IE L G   L I+AG  H
Sbjct:    1 MYSWGLGNHGQLGLGAIEETNVGIPSLVN-LDEDIDV-ISAGSEHTAVLTTEGKIYTCGSNDYGQLGHSSKQSRF--EKVELLNSYSIKSVVCGGQHTLALDEW-------------------GKVFSWGSDTRGQTGHDDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEH 203          

HSP 8 Score: 88.5817 bits (218), Expect = 2.373e-17
Identity = 64/236 (27.12%), Postives = 114/236 (48.31%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKN--LHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELG----QGDTGCLYTPKLINFFD---GVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG 551
             LT + K+Y+ G+  +G+LGH  K+      E+ +   I  +  G  H  A+   G +++WG  + G+ GH DG     P ++K+  L NV  ++ G  H+L ++  G +++++G    G+LG     GD        L N  +   GV    + A     + +++SG ++ WGK  +         +S  P+ I  + S  I  +S G  H +V+ E+  V+S+G   +G+ G G
Sbjct:   46 VLTTEGKIYTCGSNDYGQLGHSSKQSRFEKVELLNSYSIKSVVCGGQHTLALDEWGKVFSWGSDTRGQTGHDDGNKD-TPKIIKSLGLQNVSQIAAGHYHSLALTNKG-KIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGTVYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGHG 279          

HSP 9 Score: 75.0998 bits (183), Expect = 2.557e-13
Identity = 49/185 (26.49%), Postives = 94/185 (50.81%), Query Frame = 0

Query:  391 LYTWGEGSLGRLGHKDGIVH----FVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSS------TVPKWINSIS--SISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSL-KVKNPKR 562
            +Y+WG G+ G+LG   G +      +P+LV    ++  +S G++HT +++ +G ++++ G  + G+LG       +  + +   +   +K V+   + +LAL   G ++ WG      +         PK I S+   ++S ++ G +H + +     +Y+WG NTYG+ GLG  S  KV  P+ 
Sbjct:    1 MYSWGLGNHGQLGL--GAIEETNVGIPSLVNLDEDIDVISAGSEHTAVLTTEG-KIYTCGSNDYGQLGHSSKQSRF--EKVELLNSYSIKSVVCGGQHTLALDEWGKVFSWGSDTRGQTGHDDGNKDTPKIIKSLGLQNVSQIAAGHYHSLALTNKGKIYAWGDNTYGQLGLGRESGDKVVKPQE 180          

HSP 10 Score: 64.3142 bits (155), Expect = 5.068e-10
Identity = 52/193 (26.94%), Postives = 95/193 (49.22%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLA---DLGSSSIPPKYA-----DSFGGSSSVI--CGLQVTFALQPNGSLYACGEGSYGKLGLGN-SDDYFS-----LTPVVTLLLS----HNLVX-----VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHS-AVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQI 2709
            K  +Y  G+   GQL    + G   + P+       +S  G + ++   G +    +  +G++Y+ G+ SYG+LGLG+ ++ YF      L  ++   +S    H++V      VFS+G G  G+LGHG+      P+ +  L G   + +A G  H+ A++  + ++++FG G  G+LG    +    P+ I
Sbjct:  151 KGKIYAWGDNTYGQLGLGRESGDKVVKPQEGLSNVIESLIGVAPLMIAAGAEHXMIVTISGTVYSWGKNSYGQLGLGDVNNSYFPRQIRLLRSLIIKFISCGNDHSVVLTEDGGVFSFGQGRFGQLGHGSFNNELLPRKVMELMGTAVSQIACGARHTLALIPSRERLYSFGLGGSGQLGREAXENVNIPQII 343          

HSP 11 Score: 53.9138 bits (128), Expect = 8.574e-7
Identity = 44/173 (25.43%), Postives = 80/173 (46.24%), Query Frame = 0

Query: 2684 IFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYF--VTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++++G G+ G+LG G  +  ++   I SL      +  ++ G EHT +                     T G ++ CG++ +  +  +S     +  + +    ++  +  GG H LAL + G V+ WG +  GQ G++ G     P II+ L  + + QI+AG  HS A T+
Sbjct:    1 MYSWGLGNHGQLGLGAIE--ETNVGIPSLVNLDEDIDVISAGSEHTAV-------------------LTTEGKIYTCGSNDYGQLGHSSKQSRFEKVELLNSY-SIKSVVCGGQHTLALDEWGKVFSWGSDTRGQTGHDDGN-KDTPKIIKSLGLQNVSQIAAGHYHSLALTN 150          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000009364 (pep:novel supercontig:LSalAtl2s:LSalAtl2s599:282350:303566:-1 gene:EMLSAG00000009364 transcript:EMLSAT00000009364 description:"maker-LSalAtl2s599-augustus-gene-3.28")

HSP 1 Score: 114.39 bits (285), Expect = 5.982e-25
Identity = 102/390 (26.15%), Postives = 180/390 (46.15%), Query Frame = 0

Query: 2973 PQVTVRRLFPIG------SSSIFSQIAEQVFYIDPKELRL-------PYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDK-YLLNSDKNSKVDM-RHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY----FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEM--MPQVLAVRKGLSALAPYPI-LKLLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSR--------LPTHAFDVS-QRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAI 3330
            P++++  +  IG      S + F Q  + +  +D ++L +       P     VK++GE      G F   +S   QEL    + L  P      ++GS +   + L SD+    +  ++  F G L G+ALR   P+A+NL P  WK + + +    DL D D + S  +  +    N +  E+ +   DY       +  SG E  L PG   I LS+ N++EY++A    + +EM    + + ++ GL+++ P  I L L     IE+L+CG +   +  +K+   Y+  +  S+  + + W +LE  +      F+ F S + R        LP +   ++         + P+      +TC F ++LP Y+S EIMR ++I+AI
Sbjct: 4020 PELSLNPVEDIGITKEDSSQTWFVQAMKHLANVDSQQLCVNFATGGDPELPLNVKMIGEEVFGNSGSFRHFLSRTTQELLSRAIPLFVP------NLGSERKGLWTLKSDEVLSYNTEKYLKFFGQLLGMALRAGIPLALNLMPQNWKSLADCQLNDCDLRDFDPNTSNLISSIKNIKNQEEFEEFI--EDYQFPKMTVLEVSGNERELIPGGGSIYLSWSNRNEYIEALKKFKMQEMECTERWIHIKAGLASVIPMDIFLSLFKPSEIEALICGPNRVNIRYIKEHTIYQVGIRESDAHVRYFWSVLETLSKSQIQKFIKFCSNQDRIQTWSEENLPPYPMKIAPANGNSDEDDDPDQRYIRVETCMFMIKLPRYSSYEIMRKKIIFAI 4401          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000007514 (pep:novel supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13")

HSP 1 Score: 112.849 bits (281), Expect = 7.344e-25
Identity = 102/334 (30.54%), Postives = 158/334 (47.31%), Query Frame = 0

Query: 3013 VKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKV----DMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF----KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            ++  GE   D GGV      E    LSK++ N       G+ +  S  D Y L  +  S V     + +F F+G   G+A    K +        +K++L       D+E VD     SL ++  + +P   E +    +   G + + RE  L PG + I ++ ENKDEY+   +  RF    ++ M   LA   G + L P   +K+     +E L+CG  S  +   K+   Y+ D  P++ VI W W ++  FNN+++   L FV+G SR+P + F      +  Q F I R   P +  P A TCF +L LPPY S + +R+RLI AI+
Sbjct:  427 IEFDGEIGLDYGGV----AREWFYLLSKEMFNPYY----GLFEY-SATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIREN-DPQELELTFQVDEENFGTTVT-RE--LKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLA---GFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIERWGNP-SSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIE 743          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000006171 (pep:novel supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36")

HSP 1 Score: 100.523 bits (249), Expect = 5.485e-21
Identity = 97/400 (24.25%), Postives = 177/400 (44.25%), Query Frame = 0

Query: 2955 MQTLMSTMTNSNVLRNYGP-QVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVAT---------SFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQ---------RFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            ++ L S + N      Y P +V+ + +F       + QI +  + +  K+LR+ +R+       E   D GGV  + +  +  E+      L            S++D Y L  + +S V+  H   F F+G + G+A+     I        +K++L       D+E VD    +SL ++ +         +F+ ++E   L        +  +G+++P+         +  NK EYVK  +  R+   +  Q L+++KG + L P  +LK    + +E ++ G  +  +   K   R +  D    V+ W W I++ ++++++   L FV+G SR+P   F   Q          F +     P + LP A+TCF +L LP Y S EIM  +L  A++
Sbjct:  557 IKVLRSXLNNXQPQNGYCPLEVSRQEVF----EDSYRQIVKMRYKVMRKKLRVKFRN-------EEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLT---KVYELKENGKDIPV---------NESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVE 924          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000004991 (pep:novel supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39")

HSP 1 Score: 98.2117 bits (243), Expect = 4.345e-20
Identity = 94/383 (24.54%), Postives = 160/383 (41.78%), Query Frame = 0

Query: 2968 LRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSA-------LAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTI 3336
            LR     V VRR       ++F +   ++    P+E +     + +   GE   DAGG+  +    + +E+   +  L   +P      G      +  S + +   + +F F+G +   A+   K +        +K IL     + D+E  D ++ + L ++      D   D    T+      F   E+  L P    I ++ E K +Y+      R    M    A+RK LSA       + P  ++ +   Q +E L+ G  +  +  LK    Y     ++  I+W W  L  F+   K  F+ FV+G S++P   F      +  Q+FQI R +R  + LP+A TCF QL LP Y + + +R  L+ AI  C  +
Sbjct: 2596 LRREDLAVHVRR------ENVFEESFRELXRRSPEEXK---NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSP------GDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEI---QEFGVTEIRDLIPNGRNITVTEETKLDYI------RLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEV 2954          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000011039 (pep:novel supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41")

HSP 1 Score: 95.5153 bits (236), Expect = 1.395e-19
Identity = 74/294 (25.17%), Postives = 140/294 (47.62%), Query Frame = 0

Query: 3058 SNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVP--LYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMD 3339
            +N++ Y L  +  S V+  H   F F+G    +AL   K I        +K +L  K   KD+E +D  Y  SL ++      +N E+  L   + +     G   P  L PG  +++++ ENK+EY++     R  + +  Q  +  +G + + P   L+    + +E ++CG     +   ++ + YR    +++ I W W+ +   +++ +   L FV G  R+P   F      + +QRF + +V + ++ LP + TCF +L LPPY S + +  +L +AI+      ++
Sbjct:  587 ANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKD----NNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQRFCVEKVGK-DSWLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEETEGFGLE 875          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000010746 (pep:novel supercontig:LSalAtl2s:LSalAtl2s712:393080:397981:-1 gene:EMLSAG00000010746 transcript:EMLSAT00000010746 description:"maker-LSalAtl2s712-snap-gene-4.20")

HSP 1 Score: 87.4261 bits (215), Expect = 6.793e-17
Identity = 64/176 (36.36%), Postives = 92/176 (52.27%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV--GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINF---FDGVIMKKVIASNRFSLALSVSGDLWIWGKTP 501
            LALTE   VYSWG   +G+LGHG+  D   P  I SL    I+ ISAG +H+ A+  +G +++WG G  G+LGH D     +P  V +L  V    +  G  H++ +S  G +V+SFG    G+LG G T     P   +     DG  +K + +    +LAL+  G +  WG  P
Sbjct:  731 LALTE-FGVYSWGLSKYGQLGHGNCCDQKRPALIKSLTHKHIVDISAGRYHSLALDSEGGVWSWGFGVHGQLGHDDIEDVLIPQRVLSLRKVKISQIXAGYTHSVCLSSKG-EVFSFGNNLFGQLGNGLTKKSNVPIKTDISICRDGEYIKCINSGFFHNLALTNHGRVLTWGSNP 904          

HSP 2 Score: 85.5001 bits (210), Expect = 2.323e-16
Identity = 85/322 (26.40%), Postives = 133/322 (41.30%), Query Frame = 0

Query: 2594 SLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNL-----------------VXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMD---INSSNMNIQSPKKIPKLENMVD-IAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIE-ELSGKGILQISAGKNHSAAWTSPPMPT----NFNNSSPTKPR-HNIGRPNSLPDEFVSLLP 2888
            SL++ G  + G LG G+  + F     V     +N+                   V+SWG  + G+LGHGN    +RP +I SL+      ++ G  HS  L  +G ++++G G  G+LGH + +    P+++ SLR   ++ +  G  H+            +CL        + G+VF  GN+ F  +       SN+ I++   I +    +  I  G  H LAL+  G V  WG N               P II  E   K  L +   +N   A +S    T    N N+  P+     +I +   LPDE   L P
Sbjct:  683 SLFSWGSTTKGALGRGSYSENFETIKEVPYFRENNINVISVAAGTYHSLALTEFGVYSWGLSKYGQLGHGNCCDQKRPALIKSLTHKHIVDISAGRYHSLALDSEGGVWSWGFGVHGQLGHDDIEDVLIPQRVLSLRKVKISQIXAGYTHS------------VCLS-------SKGEVFSFGNNLFGQLGNGLTKKSNVPIKTDISICRDGEYIKCINSGFFHNLALTNHGRVLTWGSN---------------PQIIRLETQSKKRLLLQNLRNSRMATSSSKEATVIQNNVNDEPPSISNIKDIKKQQILPDEMPHLRP 970          

HSP 3 Score: 80.4925 bits (197), Expect = 6.975e-15
Identity = 69/254 (27.17%), Postives = 123/254 (48.43%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP-------PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVP-KWINSISS----ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
             I  +   + ++SWG+ + G LG G   + NF + I  +P        ++ ++AG +H+ A+T  G +Y+WG    G+LGH +      P L+K+L   ++  +S G  H+L +  +G  VWS+G G +G+LG  D   +  P+ +     V + ++ A    S+ LS  G+++ +G   +          S VP K   SI      I  +++G FH + +  +  V +WG N        +  L+ ++ KRL
Sbjct:  674 LIRKIGSRYSLFSWGSTTKGALGRGSYSE-NF-ETIKEVPYFRENNINVISVAAGTYHSLALTEFG-VYSWGLSKYGQLGHGNCCDQKRPALIKSLTHKHIVDISAGRYHSLALDSEG-GVWSWGFGVHGQLGHDDIEDVLIPQRVLSLRKVKISQIXAGYTHSVCLSSKGEVFSFGNNLFGQLGNGLTKKSNVPIKTDISICRDGEYIKCINSGFFHNLALTNHGRVLTWGSNPQ------IIRLETQSKKRL 917          

HSP 4 Score: 69.3218 bits (168), Expect = 2.182e-11
Identity = 61/208 (29.33%), Postives = 96/208 (46.15%), Query Frame = 0

Query:  375 ISAGHHHAAAITLDGY---LYTWG---EGSLGRLGHKDGI--VHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNT-------SSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDI 565
            ++AG +H   I   G    L++WG   +G+LGR  + +    +  VP   +N  NV SV+ G  H+L +++ G  V+S+G    G+LG G+      P LI       +  + A    SLAL   G +W WG                +P+ + S+    IS +  G  H + +     V+S+G N +G+ G GLT  K   P + DI
Sbjct:  665 LAAGGNHVLLIRKIGSRYSLFSWGSTTKGALGRGSYSENFETIKEVPYFRENNINVISVAAGTYHSLALTEFG--VYSWGLSKYGQLGHGNCCDQKRPALIKSLTHKHIVDISAGRYHSLALDSEGGVWSWGFGVHGQLGHDDIEDVLIPQRVLSLRKVKISQIXAGYTHSVCLSSKGEVFSFGNNLFGQLGNGLTK-KSNVPIKTDI 869          

BLAST of EMLSAG00000002245 vs. L. salmonis peptides
Match: EMLSAP00000010806 (pep:novel supercontig:LSalAtl2s:LSalAtl2s716:155313:159814:-1 gene:EMLSAG00000010806 transcript:EMLSAT00000010806 description:"maker-LSalAtl2s716-augustus-gene-1.11")

HSP 1 Score: 75.485 bits (184), Expect = 2.090e-13
Identity = 58/196 (29.59%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGN-TERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSG-DVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFY 2825
            V+SWG G  G+LGHG  TE   +P ++ SL     T +  G   S   +D G I T G+G  G LGHG++ +  +P+ I +L    V+ ++CG EH  +  +  E  +    GR     L  G +   C   K   M +N+  M I            V++   G+  + L   G ++  G N   +LG +   FY
Sbjct:  556 VYSWGLGSKGQLGHGTLTEYQDKPLVVDSLKAKSITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSGTPKLIEALLSVDVSAISCGSEHVVMVDSRGEVYS---WGRGLYGRLGQGHEEDSCSPKK---MSLNTDEMYI------------VNVKCNGNGTMLLCDKGTLYACGENRYNRLGLDDQSFY 733          

HSP 2 Score: 70.8626 bits (172), Expect = 5.819e-12
Identity = 63/251 (25.10%), Postives = 108/251 (43.03%), Query Frame = 0

Query: 2595 LYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV----------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFS--GMDINSSNM------NIQSPKK---IPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG 2818
            +Y+ G GS G+LG G   +Y     VV  L + ++  +                 + GDG  G LGHG+      PK+I +L     + ++ G+ H  ++  +G+++++G G  GRLG G+ +   SP+++S                  +N      V   C G    L    G ++ CG ++++  G+D  S  M       +  P K   + K   +VD  +G  H + ++    V  +G N  GQLG
Sbjct:  556 VYSWGLGSKGQLGHGTLTEYQDKPLVVDSLKAKSITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSGTPKLIEALLSVDVSAISCGSEHVVMVDSRGEVYSWGRGLYGRLGQGHEEDSCSPKKMS------------------LNTDEMYIVNVKCNGNGTMLLCDKGTLYACGENRYNRLGLDDQSFYMFGNHVDKVLVPTKLSSVSKKYKIVDYDLGMGHTVCITHDRKVVTFGKNTDGQLG 788          

HSP 3 Score: 61.6178 bits (148), Expect = 3.930e-9
Identity = 46/158 (29.11%), Postives = 76/158 (48.10%), Query Frame = 0

Query: 2668 VAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNF-QTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGM---DINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG 2818
            V+   +H   LT +  ++++G G +G+LGHG   + +  P  + SL+                     +S+TKIC G  FS+F + +G +  CG+  F  +   D NSS     +PK I  L   ++  I+ G +HV+ +   G V+ WG    G+LG
Sbjct:  540 VSSSGTHFIALTSEFLVYSWGLGSKGQLGHGTLTEYQDKPLVVDSLKA--------------------KSITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSG----TPKLIEALLSVDVSAISCGSEHVVMVDSRGEVYSWGRGLYGRLG 673          

HSP 4 Score: 59.3066 bits (142), Expect = 1.969e-8
Identity = 49/200 (24.50%), Postives = 91/200 (45.50%), Query Frame = 0

Query:  363 PKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVS--CGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSISS---ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGL 550
            P ++ +   I+ +S+   H  A+T +  +Y+WG GS G+LGH     +    LV +     S++  C  +   + + D   + + G G+ G LG GD     TPKLI     V +  +   +   + +   G+++ WG+  +          S  PK ++  +    I NV   G   +++ +  ++Y+ G N Y + GL
Sbjct:  528 PIQLPTSIRIIEVSSSGTHFIALTSEFLVYSWGLGSKGQLGHGTLTEYQDKPLVVDSLKAKSITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSGTPKLIEALLSVDVSAISCGSEHVVMVDSRGEVYSWGRGLYGRLGQGHEEDSCSPKKMSLNTDEMYIVNVKCNGNGTMLLCDKGTLYACGENRYNRLGL 727          

HSP 5 Score: 59.3066 bits (142), Expect = 2.036e-8
Identity = 45/160 (28.12%), Postives = 75/160 (46.88%), Query Frame = 0

Query:  403 GHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGD-TGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSG--------DLWIWGKTPWNTSSTVPKWINSISSI--SNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            GH+ G+    P  +     +  VS    H + ++ + + V+S+G G+ G+LG G  T     P +++      + K+ A   FS+  S +G             G   WN+S T PK I ++ S+  S +S G  H++++     VYSWG   YG+ G G
Sbjct:  519 GHESGL-RLTPIQLPTSIRIIEVSSSGTHFIALTSEFL-VYSWGLGSKGQLGHGTLTEYQDKPLVVDSLKAKSITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSGT-PKLIEALLSVDVSAISCGSEHVVMVDSRGEVYSWGRGLYGRLGQG 675          

HSP 6 Score: 56.6102 bits (135), Expect = 1.281e-7
Identity = 63/250 (25.20%), Postives = 101/250 (40.40%), Query Frame = 0

Query:  283 VEKIETGSSCTYVIHTNGKVSCWKDNHFS-------NVHFPSPIIDASLSQD------NESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITL---DGYLYTWGEGSLGRLG-----------HKDGIVHFVPTLVKNL---HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLY----TPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWG 498
            + KI  G   +     NG +    D  F        N      +I+A LS D          ++ +    +VYSWG G +GRLG G ++D   PK++S     M I     +     L    G LY  GE    RLG           H D +   VPT + ++   + +     G  HT+ ++ D  +V +FG   +G+LG+G T  +     +P+ +       +  V     F+L  +    L++WG
Sbjct:  590 ITKICAGEGFSVFASDNGIIMTCGDGSFGCLGHGDWNSSGTPKLIEALLSVDVSAISCGSEHVVMVDSRGEVYSWGRGLYGRLGQGHEEDSCSPKKMSLNTDEMYIVNVKCNGNGTMLLCDKGTLYACGENRYNRLGLDDQSFYMFGNHVDKV--LVPTKLSSVSKKYKIVDYDLGMGHTVCITHD-RKVVTFGKNTDGQLGRGHTRNMVSEGGSPESVKSLIDKEVSFVACGPTFTLIGTNENVLYVWG 836          

HSP 7 Score: 55.0694 bits (131), Expect = 3.524e-7
Identity = 47/179 (26.26%), Postives = 78/179 (43.58%), Query Frame = 0

Query: 2577 SSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLG--------HGN-TERCRRPKIISSLSGNFATF-VAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQT----KKSPEQISSLRGYFVTYVACG 2723
            S++ CG +    +   G +Y+ G G YG+LG G+ +D  S +P    L +  +  V                  ++ G+    +LG         GN  ++   P  +SS+S  +      +G  H+  +T   K+ TFG    G+LG G+ +       SPE + SL    V++VACG
Sbjct:  643 SAISCGSEHVVMVDSRGEVYSWGRGLYGRLGQGHEED--SCSPKKMSLNTDEMYIVNVKCNGNGTMLLCDKGTLYACGENRYNRLGLDDQSFYMFGNHVDKVLVPTKLSSVSKKYKIVDYDLGMGHTVCITHDRKVVTFGKNTDGQLGRGHTRNMVSEGGSPESVKSLIDKEVSFVACG 819          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|296434522|sp|Q15751.2|HERC1_HUMAN (RecName: Full=Probable E3 ubiquitin-protein ligase HERC1; AltName: Full=HECT domain and RCC1-like domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HERC1; AltName: Full=p532; AltName: Full=p619)

HSP 1 Score: 783.482 bits (2022), Expect = 0.000e+0
Identity = 445/1109 (40.13%), Postives = 641/1109 (57.80%), Query Frame = 0

Query: 2321 SDPYILASGSSKNPGGIVNIWSILSQSVIRTL-IGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCS----------LPD------SRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKD--VNLKD-IVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            SD   L SG     GG++NIWS+   SV++T+ IG G I    WI    +A     +++V ++N   E    N ++A+ CR  L +        AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L+L+ +LC+          LPD      + W WL  F+ ++     L   N     E       + + +D  V L D   W    D  +M W    P DW  GGK  VYL G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK+FTFGNGD GRLG GN   KK PE++++L GY +  VACGL HT                                 KI+      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P++IP L  ++  D+AVG +H LAL+ +G V+ WG N  GQLG       +EP ++  L GK + QISAG+ HSAAWT+PP+P      S P +    +G P+++P ++ +L    S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAPLVWK +  +  T +DLE+VDL Y ++L+ +    +    E+S   ++P D F+G S  G+ VP+ PG   I L++ N+ EYV+ A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W  LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3757 SDGLALVSGGL---GGLMNIWSLRDGSVLQTVVIGSGAIQTTVWIPEVGVAACSNRSKDVLVVNCTAEWAAANHVLAT-CRTALKQQGVLGLNMAPCMRAFLERLPMMLQEQYAYEKPHVVCGDQLVHSPYMQCLASLAVGLHLDQLLCNPPVPPHHQNCLPDPASWNPNEWAWLECFSTTIKAAEALT--NGAQFPESFTVPDLEPVPEDELVFLMDNSKWINGMDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKIDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQIPVLAGVIIEDVAVGAEHTLALASNGDVYAWGSNSEGQLGLGHTNHVREPTLVTGLQGKNVRQISAGRCHSAAWTAPPVPPRAPGVSVPLQ----LGLPDTVPPQYGALR-EVSIHTVRARLRLLYHFSDLMYSSWRLLNLSPNNQNSTSHYNAGTWGIVQGQLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPLVWKQLCCVPLTLEDLEEVDLLYVQTLNSILHIEDSGITEESFHEMIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVERAIEYRLHEMDRQVAAVREGMSWIVPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWHTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQLVMAERLRYAINNCRSIDMDNYMLSRN 4850          

HSP 2 Score: 330.487 bits (846), Expect = 6.734e-89
Identity = 242/760 (31.84%), Postives = 372/760 (48.95%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW----SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKA------------VQLAQG--------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF------DEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W    SGP     L + VI TA  ++ S PPLSL     +PP+G   L  V TFL     IP +           ELLL LA QRG    +L WI  AL  +    ++    ++ ++             +Q+ +               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+    +D + IE G  CT+VI T+G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I        ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK I  +++  I +VS G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT     +    +H PY  + +  TFS     L +F L  +  K+NS           E   FL     LL ++    ++  + +     +   L +++F+ +D   +  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLS--LCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLK-GVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMALGASAVVHTMEKGKLLSSQEGMISFDCFMTILMQMRRSLGSSADRSQWREPTRTSDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDVSIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSH----LRSF-LERYCDKINSEIPPLPFPSSREHHSFLKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDS-TVPDEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 174.096 bits (440), Expect = 1.521e-41
Identity = 121/389 (31.11%), Postives = 188/389 (48.33%), Query Frame = 0

Query:  249 LTEQIVHF-TSR-------NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-----------NTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWT 582
            + EQI+ + TSR        K D+Y WG   H            P      +  +++  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG L+T+G G  GRLG  +     +P  V  L  + +G V+CG  HTL VS DG  VW+FG G+ G+LG G++    +P+ I+   G+ +KKV    +FS+AL+  G ++ +G+              N    +P     I  I +V+ G  H + +  N  VY+WG N+ G+ GLG T+  V+ P     L   ++  I AG   S AWT
Sbjct: 3976 MDEQIMSWATSRPEDWHLGGKCDVYLWGAGRHGQLAEAGRNVMVPAAAPSFSQAQQVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTSDGKLFTFGNGDYGRLGLGNTSNKKLPERVTALEGYQIGQVACGLNHTLAVSADGSMVWAFGDGDYGKLGLGNSTAKSSPQKIDVLCGIGIKKVACGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQIPVLAGVI--IEDVAVGAEHTLALASNGDVYAWGSNSEGQLGLGHTN-HVREPTLVTGLQGKNVRQISAGRCHSAAWT 4359          

HSP 4 Score: 173.326 bits (438), Expect = 2.411e-41
Identity = 129/369 (34.96%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD-----------YFSLTPVVTLLLS--HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQL--GNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++           + S+  V +   S  H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +T+ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  V          K+C G   SL LTS G V+  G     G    SS      PK I +L    +VD+++G  H LALS    V+ WG N +GQ   GN +G   K P  +  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLIEELAATRIVDVSIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITK-PKKVSGLDGIAIQQISAGTSHSLAWTALP 737          

HSP 5 Score: 110.538 bits (275), Expect = 2.220e-22
Identity = 138/648 (21.30%), Postives = 277/648 (42.75%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEESDELNIE-NFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISN----NLRSCVLRLIFQSILLARNVSKKLLNEPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNS---------------------KSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSLISQSEKL-----------------------RGSIDNVLE--------AIYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED 1551
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT          ++L   LQ +      +L+          L+I  ++   Y ++++   + +  L  LC+ L N +S     ++ S             + V      E   ++ +L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E G   +    IV +LFS + +  W +                     K  +L+  +       + F+ + +  C++++ + +       GY +A+  ++ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD I C+L+ E  ++SF KNG    LAF D+ ++ ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T+++IR L+  ++  W      C +  +++    +K  +K     + +S  +                       R  + ++ E         ++P+LA++  VD+GL +G    +K+  R   ++G+    S + K++W++
Sbjct: 1736 LERALQA-NKHHIEAQQRLLLVTVFALSVHYQPVDVSLAISTGLLNVLSQLCGTD---------TMLGQPLQLLPKTGVSQLSTALKVASTRLLQILAITTGTY-ADKLSPKVVQSLLDLLCSQLKNLLSQTGVLHMASFGEGEQEDGEEEEKKVDSSGETEKKDFRAALRKQHAAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIASQKCSSGIPLVGNLRTRLLALHVLEAVLPACESGVEDDQMAQIVERLFSLLSDCMWETPIAQAKHAIQIKEKEQEIKLQKQGELEEEDENLPIQEVSFDPEKAQCCLVENGQILTHGSGGKGYGLASTGVTSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDFITCVLDMEARTISFGKNGEEPKLAFEDVDAA-ELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVAAVLAEATIQLIRILH--RTDRW----TYCINKKMMERLHKIKICIKESGQKLKKSRSVQSREENEMREEKESKEEEKGKHTRHGLADLSELQLRTLCIEVWPVLAVIGGVDAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE 2351          

HSP 6 Score: 92.4337 bits (228), Expect = 6.418e-17
Identity = 81/278 (29.14%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQ-TKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+I +L G F   V  G+  S  LT  G+++ +G G    LG G+ + T   P+ I  L    +  V+ G  H              CL  +        +V+  GN+        +S   I  PKK+  L+ +    I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGSSEATALRPKLIEELAATRIVDVSIGDSH--------------CLALSH-----DNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLA 732          

HSP 7 Score: 61.6178 bits (148), Expect = 1.319e-7
Identity = 80/349 (22.92%), Postives = 152/349 (43.55%), Query Frame = 0

Query: 1996 AKNKLPSFLALCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWKDNEYKRIL-------IIDFDDESTL-EISVMSDQILTWSPSGKYLVLSVGNIIYIW---------DMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGT-----------------------SISITTLSWSSNSDYLAIGYENGEILV 2295
            A N   +   L  +C  NL++ +   +  TV++         ++  I  E  +V S  +  +  +  LL +  ++  P +  S    +K+   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +      +  +D+ G L +  +  +L  H +++++    N   +LAT   DGTIR+W   + +  L        ++ D E +L   S  S   ++WS SGKYL  ++  ++ IW         D++    +    P+  P     WS  S  E+LL  R +G+                        +S+T ++W S     A+GY +G++L+
Sbjct: 3274 ASNAPSAAKLLVQLCTQNLISAATGVNLTTVDDSIQRKFLPSFLRGIAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKSEV--EKKG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQTT-LQTLADMGGDLRKCSF-IKLEAHQNRVMTCVWCNKKGLLATSGNDGTIRVWNVTKKQYSLQQTCVFNRLEGDAEESLGSPSDPSFSPVSWSISGKYLAGALEKMVNIWQVNGGKGLVDIQPHWVSALAWPEEGPAT--AWSGESP-ELLLVGRMDGSLGLIEVVDVSTMHRRELEHCYRKDVSVTCIAWFSEDRPFAVGYFDGKLLL 3608          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|341941077|sp|Q4U2R1.3|HERC2_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HERC2; AltName: Full=HECT domain and RCC1-like domain-containing protein 2; AltName: Full=HECT-type E3 ubiquitin transferase HERC2)

HSP 1 Score: 458.373 bits (1178), Expect = 1.191e-127
Identity = 311/1019 (30.52%), Postives = 497/1019 (48.77%), Query Frame = 0

Query: 2416 AFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKK-------DVNLKDI------VWTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A +K     +  Q+EYE   +  G QL++S F + L  LA  L L+T+ C     +W W   +  +      L+KR        L  LF DE+ K       D    D+      ++  E D  L++W    P DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  I G Q  FA+  +G LYA G G+ G+LG+G ++     TP +   + H  +                    V+SWG+ EDGKLGHGN   C RP++I SL G     VA G +HSA +T  G ++T+G G  GRLGH + + +  P+ + +L+G+ V  +ACG        L      ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ P+++  L+   ++ IA G  H +  ++ G V+ WG N  GQLG+ +    + P ++  L GK + +++ G  H+ AW++            +KP      P  +P E+  L  +P  +    L  L  + E+    + ++ L   +D     P        ++  D +R  L          + + +TM      R +GP V + R+             P G+ S+F Q+  ++    P  L LP+R WKVK VGE+ DD GG + ++I+E+C+EL   +  LL  TPNG  + G+N+D YLLN    + V    F FLGVL G+A+RT  P+++NLA  VWK +  +  T  DL +VD  +   L Y+  +     E +++ LP   F   S SG+++ L   S+   ++ +N+ EYV+ A+N R  E   QV AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ ++PS  ++ W WE++E F+N  + LFL FV GR+RLP    D   R  +++V      P++ LP + TCFF L+LP Y+ K+++  +L YAI  C++ID D Y
Sbjct: 3817 ALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTP------LPRLFLDEVAKKIRELMADSESMDVLHESHSIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVS--TPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLG-HGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKVNRVACGSAHTLAWST------------SKPASAGKLPAQVPMEYNHLQEIPIIALRNRLLLLHHISELFCPCIPMFDLEGSLDETGLGP--------SVGFDTLRGILISQGKEAAFRKVVQATMVRD---RQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEICEELQNGLTPLLIVTPNGRDESGANRDCYLLNPATRAPVHCSMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMSLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLP---FTVPSASGQDIQL--SSKHTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPSASLVQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDY 4798          

HSP 2 Score: 171.4 bits (433), Expect = 9.133e-41
Identity = 114/370 (30.81%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW--------------------------RESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++ V++ G     QL  L  S I  P ++++    +   V  G +  FA+   G +Y+CGE + G+LGLG S     +   +T L S+ +  V                  FSWG+G+DGKLGH +   C +P++I +L       +A G+SHSA LT  G+++T+G G+ GRLGHG+  T+  P+ +  L G+ V  VACG        L    + F+W                           + V +I  G  FSL LT SG V+  G   +  +  + S+++++ P+ +  L  + +V +AVG  H LA++ SG V+ WG N  GQ GN +    ++P +++ L G+ I +++ G +HS AWT+
Sbjct: 2959 RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLG-HGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTT 3327          

HSP 3 Score: 154.836 bits (390), Expect = 1.083e-35
Identity = 110/308 (35.71%), Postives = 158/308 (51.30%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP------IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-----WNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK-----RLDIYDIDGICAGPTQSYAWTMRKGKGCFHW------PYSNEPDAKTFSQVSDIL 610
            LAL    +VYSWG G  GRLGHGD      PK IS+         ++ I+ G  ++AAIT +G LYTWG G+ GRLGH       +P LV  L    V  V+CG+    TL V+++G QVWS+G G+ G+LG+G +    TPKLI     + + KV   ++FS+AL+  G ++ WGK         T   V  PK +  +    + +V+ G  H + + E+  V+SWG N   +C     +L+V  P+      LD   I GI  GP QS+AW+      C  W      P+  +  + TF Q+  +L
Sbjct:  509 LALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENG-QVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSND--QC-QHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQSFAWS-----SCSEWSIGLRVPFVVDICSMTFEQLDLLL 807          

HSP 4 Score: 150.984 bits (380), Expect = 1.622e-34
Identity = 106/334 (31.74%), Postives = 167/334 (50.00%), Query Frame = 0

Query:  282 DVEKIETGSSCTYVIHTNGKV-SCWKDNH------FSNVHFPSPIIDASLS---------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            +V ++  GS   + +   GKV SC +  +       S+   P P    +LS                 ALT D KV+SWG G  G+LGH  + + + P+ I +L    I  I+ G  H+AA+T  G LYTWG G  GRLGH D      P +VK L  H V  V+CG++   TL ++ +G+ V+S+G G+ G+LG+G +     P+ I   +G  + ++    +FSLAL+ SG +W WGK  +       +     P+ +  +    I +V+ G  H + + ++  VY+WG N +G+ G G T++  K    + L+   I  +  G + S AWT
Sbjct: 2994 NVVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGL-VFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWT 3326          

HSP 5 Score: 149.828 bits (377), Expect = 3.224e-34
Identity = 90/271 (33.21%), Postives = 145/271 (53.51%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI--MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ + +VYSWG    G+LGHG++   + P+ I SL  I  + ++AG  H+A +T  G LYTWG+G  GRLGH D      P LV+ L  H V  ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I+   G+ + KV   ++FS+AL+ SG ++ WGK  +       +     P+ +  +    +  ++TG  H +   E+  VY+WG N  G+ G G T+  ++ P+    L    ++ +  G   + AW+  K
Sbjct: 4054 LALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDT-VWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTN-AIQRPRLVAALQGKKVNRVACGSAHTLAWSTSK 4322          

HSP 6 Score: 124.02 bits (310), Expect = 2.028e-26
Identity = 81/265 (30.57%), Postives = 135/265 (50.94%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFA----TFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPPMPTNFNNSSPTKPRHNIGRPNSLP 2880
            V+SWG G+ G+LGHG+T     PK+IS+ SG  A      +A G+++SA +T +G+++T+G G+ GRLGHG+ + +  P  ++ L+G  V  VACG                   G   +L +T +G V+  G+  +  +    S+   ++PK I KL+++  + +  G    +AL+K G V+ WG     +LG+ +    + P ++E L GK ++ ++AG  H  A         W S     +F+    TKP      P +LP
Sbjct:  517 VYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGS------------------GDAQTLAVTENGQVWSWGDGDYGKLGRGGSD-GCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKP-----EPTALP 757          

HSP 7 Score: 102.834 bits (255), Expect = 5.451e-20
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSS------VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            VY  G G  G+L      ++P   PK   +F G  +      + CG   + A+   G LY  G G+YG+LG G+S+D     P++                    TL ++ N   V+SWGDG+ GKLG G ++ C+ PK+I  L       V  G+  S  LT  G+++++G GD  RLGHG  +  + P+ +  L+G  V  VA G  H
Sbjct:  517 VYSWGCGDGGRLGH--GDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSED--EAIPMLVAGLKGLKVIDVACGSGDAQTLAVTEN-GQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTH 721          

HSP 8 Score: 85.8853 bits (211), Expect = 7.290e-15
Identity = 72/259 (27.80%), Postives = 126/259 (48.65%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLG--HGDKKDYNFPKE-ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            +++  + + L+      +Y WG+   G+LG   G K     P E +++L P+  I  G     A+T DG LY  G G+ GRLG         PTL++++ +V     +V+ G +H L +S +G +V+S+G   +G+LG G+      P++I    G+ +  V A    S  ++ +GDL+ WGK  +       +     PK + ++     +     +G    + + ++ +V+SWG   YGK G G  S   K P ++D
Sbjct: 3939 MNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLI-GGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG-EVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRG-GSDGCKVPMKID 4194          

HSP 9 Score: 81.2629 bits (199), Expect = 1.729e-13
Identity = 65/215 (30.23%), Postives = 99/215 (46.05%), Query Frame = 0

Query: 2542 GKSSVYLVGNGQNGQLADLGSS---SIPPKYADSFGGSS-SVICGL--QVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLV----------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
             +  +Y  G G  G+L   GSS   +IP   A   G     V CG     T A+  NG +++ G+G YGKLG G SD     TP ++  L   +++                 V+SWG G++ +LGHG  E  R PK++  L G     VA G++H   LT+  ++ ++G+ D+ +       TK  P  +  L    +  +ACG   +   FAW
Sbjct:  569 AEGELYTWGRGNYGRLGH-GSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDG--CKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQS---FAW 777          

HSP 10 Score: 76.2554 bits (186), Expect = 5.229e-12
Identity = 59/206 (28.64%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  263 DIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNVHFPS------PIIDASLS-------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTL 433
            D+YTWG             D   PK+ + +     I+    +G + T  +  +  V  W D  +  +          P+   SL+       +    F +ALT+   VY+WG G + RLGHG       P+++  L    ++ I+ G  H    T DG +YTWG+   G+LG  DG  + +  P LV  L    V  V+CG+ HTL
Sbjct: 4113 DLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLG--DGTTNAIQRPRLVAALQGKKVNRVACGSAHTL 4316          

HSP 11 Score: 73.559 bits (179), Expect = 3.713e-11
Identity = 68/253 (26.88%), Postives = 121/253 (47.83%), Query Frame = 0

Query:  340 KVYSWGNGSHGRLG--HGDK-KDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGR--LGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            KV+ WG     +LG   G K K  +F + +S+L  ++ ++ G     A+T++G +Y+ GE + GR  LG   G V  +P  +  L +      +V  G +H   ++ DG +V+S+G G++G+LG         P+LI       ++ +   +  S AL+ SG+L+ WG   +       NT+   PK +  +    +  V+ G      + + +   V+SWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2961 KVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALN-VVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVP-IPRQITALSSYVVKKVAVHSGGRHATALTVDG-KVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRG-GSEGCNIPQNIERLNGQGVC 3209          

HSP 12 Score: 65.0846 bits (157), Expect = 1.232e-8
Identity = 64/263 (24.33%), Postives = 109/263 (41.44%), Query Frame = 0

Query:  220 PVIFNKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNV--------HFPSPI-----IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDG--IVHFVPTLVKNL---HNVGSVSCGAQHTLIVSKDGMQVWSFG 446
            P+   K +    G+    H +++   + Y            + ++YTWG             D   P +   +  ++ I+    +G + T  +  NG+V  W D  +  +          P  I     +D    +    F +ALT+D +VYSWG G + RLGHG ++   +PK +  L    ++ ++AG  H  A+T D  +++W  GS  +  H D   +    PT +  L   H VG ++CG   +   S      WS G
Sbjct:  535 PLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITA------EGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSW--GSNDQCQHFDTLRVTKPEPTALPGLDSKHIVG-IACGPAQSFAWSS--CSEWSIG 786          

HSP 13 Score: 62.7734 bits (151), Expect = 7.335e-8
Identity = 55/180 (30.56%), Postives = 80/180 (44.44%), Query Frame = 0

Query: 2679 TDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            T + K+F +G  D+ +LG       K P    +L    V  VA G   +K  FA                    G V+ CG +    + +  S+  +  P++I  L + V   +AV  GG H  AL+  G V+ WG    G+LG+ S     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 2957 TIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGG---SKSLFA----------------VTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTS 3117          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|308153453|sp|O95714.2|HERC2_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HERC2; AltName: Full=HECT domain and RCC1-like domain-containing protein 2; AltName: Full=HECT-type E3 ubiquitin transferase HERC2)

HSP 1 Score: 455.292 bits (1170), Expect = 1.089e-126
Identity = 312/1019 (30.62%), Postives = 497/1019 (48.77%), Query Frame = 0

Query: 2416 AFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKK-------DVNLKDI------VWTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A +K     +  Q+EYE   +  G QL++S F + L  LA  L L+T+ C     +W W   +  +      L+KR        L  LF DE+ K       D    D+      ++  E D  L++W    P DW    GG  ++Y  G+   GQL  +  + +  P   ++      V  I G Q  FA+  +G LYA G G+ G+LG+G ++     TP +   + H  +                    V+SWG+ EDGKLGHGN   C RP++I SL G     VA G +HSA +T  G ++T+G G  GRLGH + + +  P+ + +L+G+ V  +ACG        L      ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ P+++  L+   ++ IA G  H +  ++ G V+ WG N  GQLG+ +    + P ++  L GK + +++ G  H+ AW++            +KP      P  +P E+  L  +P  +    L  L  L E+    + ++ L   +D     P        ++  D +R  L          + + +TM      R +GP V + R+             P G+ S+F Q+  ++    P  L LP+R WKVK VGE+ DD GG + ++I+E+C+EL   +  LL  TPNG  + G+N+D YLL+    + V    F FLGVL G+A+RT  P+++NLA  VWK +  +  T  DL +VD  +   L Y+  +     E +++ LP   F   S SG+++ L   S+   ++ +N+ EYV+ A+N R  E   QV AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ ++PS  +I W WE++E F+N  + LFL FV GR+RLP    D   R  +++V      P++ LP + TCFF L+LP Y+ K+++  +L YAI  C++ID D Y
Sbjct: 3815 ALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTP------LPRLFLDEVAKKIRELMADSENMDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVS--TPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLG-HGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKVNRVACGSAHTLAWST------------SKPASAGKLPAQVPMEYNHLQEIPIIALRNRLLLLHHLSELFCPCIPMFDLEGSLDETGLGP--------SVGFDTLRGILISQGKEAAFRKVVQATMVRD---RQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFGPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEICEELQNGLTPLLIVTPNGRDESGANRDCYLLSPAARAPVHSSMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMSLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLP---FTVPSASGQDIQL--SSKHTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPSASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDY 4796          

HSP 2 Score: 172.17 bits (435), Expect = 5.663e-41
Identity = 115/370 (31.08%), Postives = 185/370 (50.00%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW--------------------------RESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++ V++ G     QL  L  S I  P ++++    +   V  G +  FA+   G +YACGE + G+LGLG S     +   +T L S+ +  V                  FSWG+G+DGKLGH +   C +P++I +L       +A G+SHSA LT  G+++T+G G+ GRLGHG+  T+  P+ +  L G+ V  VACG        L    + F+W                           + V +I  G  FSL LT SG V+  G   +  +  + S+++++ P+ +  L  + +V +AVG  H LA++ SG V+ WG N  GQ GN +    ++P +++ L G+ I +++ G +HS AWT+
Sbjct: 2958 RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLG-HGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTT 3326          

HSP 3 Score: 155.221 bits (391), Expect = 6.658e-36
Identity = 110/308 (35.71%), Postives = 158/308 (51.30%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP------IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-----WNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK-----RLDIYDIDGICAGPTQSYAWTMRKGKGCFHW------PYSNEPDAKTFSQVSDIL 610
            LAL    +VYSWG G  GRLGHGD      PK IS+         ++ I+ G  ++AAIT +G LYTWG G+ GRLGH       +P LV  L    V  V+CG+    TL V+++G QVWS+G G+ G+LG+G +    TPKLI     + + KV   ++FS+AL+  G ++ WGK         T   V  PK +  +    + +V+ G  H + + E+  V+SWG N   +C     +L+V  P+      LD   I GI  GP QS+AW+      C  W      P+  +  + TF Q+  +L
Sbjct:  508 LALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENG-QVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSND--QC-QHFDTLRVTKPEPAALPGLDTKHIVGIACGPAQSFAWS-----SCSEWSIGLRVPFVVDICSMTFEQLDLLL 806          

HSP 4 Score: 150.214 bits (378), Expect = 2.593e-34
Identity = 93/266 (34.96%), Postives = 145/266 (54.51%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            ALT D KV+SWG G  G+LGH  + + + P+ I +L    I  I+ G  H+AA+T  G LYTWG G  GRLGH D      P +VK L  H V  V+CG++   TL ++ +G+ V+S+G G+ G+LG+G +     P+ I   +G  + ++    +FSLAL+ SG +W WGK  +       +     P+ +  +    I +V+ G  H + + ++  VY+WG N +G+ G G T++  K    + L+   I  +  G + S AWT
Sbjct: 3061 ALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGL-VFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWT 3325          

HSP 5 Score: 149.828 bits (377), Expect = 2.940e-34
Identity = 90/271 (33.21%), Postives = 144/271 (53.14%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCI--SAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRK 585
            LAL+ + +VYSWG    G+LGHG++   + P+ I SL  I  +  +AG  H+A +T  G LYTWG+G  GRLGH D      P LV+ L  H V  ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I+   G+ + KV   ++FS+AL+ SG ++ WGK  +       +     P+ +  +    +  ++TG  H +   E+  VY+WG N  G+ G G T+  ++ P+    L    ++ +  G   + AW+  K
Sbjct: 4052 LALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDIACGSGDAQTLCLTDDDT-VWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTN-AIQRPRLVAALQGKKVNRVACGSAHTLAWSTSK 4320          

HSP 6 Score: 124.02 bits (310), Expect = 2.281e-26
Identity = 82/265 (30.94%), Postives = 135/265 (50.94%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFA----TFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPPMPTNFNNSSPTKPRHNIGRPNSLP 2880
            V+SWG G+ G+LGHG+T     PK+IS+ SG  A      +A G+++SA +T +G+++T+G G+ GRLGHG+ + +  P  ++ L+G  V  VACG                   G   +L +T +G V+  G+  +  +    S+   ++PK I KL+++  V +  G    +AL+K G V+ WG     +LG+ +    + P ++E L GK ++ ++AG  H  A         W S     +F+    TKP      P +LP
Sbjct:  516 VYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGS------------------GDAQTLAVTENGQVWSWGDGDYGKLGRGGSD-GCKTPKLIEKLQDLDVVKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKP-----EPAALP 756          

HSP 7 Score: 103.219 bits (256), Expect = 4.348e-20
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSS------VICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            VY  G G  G+L      ++P   PK   +F G  +      + CG   + A+   G LY  G G+YG+LG G+S+D     P++                    TL ++ N   V+SWGDG+ GKLG G ++ C+ PK+I  L       V  G+  S  LT  G+++++G GD  RLGHG  +  + P+ +  L+G  V  VA G  H
Sbjct:  516 VYSWGCGDGGRLGH--GDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSED--EAIPMLVAGLKGLKVIDVACGSGDAQTLAVTEN-GQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTH 720          

HSP 8 Score: 85.8853 bits (211), Expect = 5.962e-15
Identity = 73/259 (28.19%), Postives = 126/259 (48.65%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLG--HGDKKDYNFPKE-ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            +++  + + L+      +Y WG+   G+LG   G K     P E +++L P+  I  G     A+T DG LY  G G+ GRLG         PTL++++ +V     +V+ G +H L +S +G +V+S+G   +G+LG G+      P++I    G+ +  V A    S  ++ +GDL+ WGK  +       +     PK + ++     V     +G    + + ++ +V+SWG   YGK G G  S   K P ++D
Sbjct: 3937 MNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLI-GGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEG-EVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVVDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRG-GSDGCKVPMKID 4192          

HSP 9 Score: 81.6481 bits (200), Expect = 1.462e-13
Identity = 66/215 (30.70%), Postives = 99/215 (46.05%), Query Frame = 0

Query: 2542 GKSSVYLVGNGQNGQLADLGSS---SIPPKYADSFGGSS-SVICGL--QVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-VVTLLLSHNLV----------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
             +  +Y  G G  G+L   GSS   +IP   A   G     V CG     T A+  NG +++ G+G YGKLG G SD     TP ++  L   ++V                 V+SWG G++ +LGHG  E  R PK++  L G     VA G++H   LT+  ++ ++G+ D+ +       TK  P  +  L    +  +ACG   +   FAW
Sbjct:  568 AEGELYTWGRGNYGRLGH-GSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDG--CKTPKLIEKLQDLDVVKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKPEPAALPGLDTKHIVGIACGPAQS---FAW 776          

HSP 10 Score: 75.8702 bits (185), Expect = 6.340e-12
Identity = 42/109 (38.53%), Postives = 59/109 (54.13%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTL 433
            F +ALT+   VY+WG G + RLGHG       P+++  L    ++ I+ G  H    T DG +YTWG+   G+LG  DG  + +  P LV  L    V  V+CG+ HTL
Sbjct: 4208 FSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLG--DGTTNAIQRPRLVAALQGKKVNRVACGSAHTL 4314          

HSP 11 Score: 73.1738 bits (178), Expect = 4.813e-11
Identity = 69/260 (26.54%), Postives = 121/260 (46.54%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLG--HGDK-KDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGS-------VSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            A T   KV+ WG     +LG   G K K  +F + +S+L  ++ ++ G     A+T++G +Y  GE + GRLG   GI      + + +  + S       V  G +H   ++ DG +V+S+G G++G+LG         P+LI       ++ +   +  S AL+ SG+L+ WG   +       NT+   PK +  +    +  V+ G      + + +   V+SWG   +GK G G  S     P+ ++  +  G+C
Sbjct: 2954 AATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALN-VVQVAGGSKSLFAVTVEGKVYACGEATNGRLGL--GISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDG-KVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRG-GSEGCNIPQNIERLNGQGVC 3208          

HSP 12 Score: 68.5514 bits (166), Expect = 1.200e-9
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTL 433
            F LALT+   V++WG G + RLGHG       P+ +  L    I+ ++ G  H  A+T  G +Y WG+   G+ G+    V+  PTLV+ L    +  V+CG+ H++
Sbjct: 3216 FSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSV 3322          

HSP 13 Score: 64.3142 bits (155), Expect = 2.448e-8
Identity = 37/123 (30.08%), Postives = 61/123 (49.59%), Query Frame = 0

Query:  288 TGSSCTYVIHTNGKVSCWKDNHFSNV--------HFPSPI-----IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWG 395
            +G + T  +  NG+V  W D  +  +          P  I     +D    +    F +ALT+D +VYSWG G + RLGHG ++   +PK +  L    ++ ++AG  H  A+T D  +++WG
Sbjct:  612 SGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWG 734          

HSP 14 Score: 64.3142 bits (155), Expect = 2.469e-8
Identity = 59/198 (29.80%), Postives = 87/198 (43.94%), Query Frame = 0

Query: 2661 SGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            SG+     A G   +A +  + K+F +G  D+ +LG       K P    +L    V  VA G   +K  FA                    G V+ CG +    + +  S+  +  P++I  L + V   +AV  GG H  AL+  G V+ WG    G+LG+ S     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 2940 SGSLIRKKAAGLESAATI--RTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGG---SKSLFA----------------VTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTS 3116          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|91206848|sp|Q9VR91.3|HERC2_DROME (RecName: Full=Probable E3 ubiquitin-protein ligase HERC2; AltName: Full=HECT domain and RCC1-like domain-containing protein 2; AltName: Full=HECT-type E3 ubiquitin transferase HERC2)

HSP 1 Score: 416.001 bits (1068), Expect = 7.481e-115
Identity = 295/912 (32.35%), Postives = 441/912 (48.36%), Query Frame = 0

Query: 2524 DSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRR------------LFPI-GSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE-TRPTGGGD 3356
            D+ L++W    P DW    GG S++Y  G+   GQL  L  S I    P  A S      +  G Q  FA+ P+G L+A G GS G+LG+G SD +    P +   L H  V                    V++WG+GEDGKLGHGN     RPK++  L+G     +A G++HSA +T  G + T+G G  GRLGHG+ + +  P+ + +L GY    +ACG    +          ++W +                           V K+  G  FS+ LT SG V+  G   F  +   S + +++ PKK+  L+   ++ IA G  H +A S SG V+ WG N  GQLG+ +    + P ++  L GK I++++ G  H+ A ++  +                 RP   P     L+   +      RL LLH  S  +     + P I  D             +S  A+++ L   +      + + +TM      + +GP + + R            L  I G  S+F Q+ +++  +  + L LP+R WKVK VGE+ DD GG + ++I+EMC EL    + LL  TPNG  + G+N+D +LL+   +S + M  F FLGVL G+A+RT  P++INLA  VW+ +        DL +VD  Y   L  +    N D++       +  F   S  G EVPL   +    +S  N+ EYV+ AL  R  E   QV AVR G+S + P P+L L  +  ++++VCG    PL +LK +A Y+  DPS+ ++ W WE++EEF N  + LFL FV GR+RLP    D   R    Q+L    P++ LP + TCFF L++P Y+ K ++  +L YAI  C++ID D Y      E T  TG  D
Sbjct: 4027 DAQLLQWLNRRPDDWALSWGGASTIYGWGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWA--IPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVD-HVRRPKKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDGTVTAIQRPRLVAALQGKHIVKVTCGSAHTLALSTSQLSERL-------------RPLPNPPLEYDLVRDLAPEALHARLILLHHFSELVCPCLAMLP-ISGD-------------LSLGALKDVLVYNIKEAAFRKVIQTTMVRD---KQHGPVIELNRIQVKRSRNRCNGLAGIDGMKSVFGQMVQKLPLLTQEALALPHRVWKVKFVGESVDDCGGGYSESIAEMCDELQNGSVPLLINTPNGRGEAGANRDCFLLDPTLSSVLQMNMFRFLGVLMGIAVRTGSPLSINLAEPVWRQLTGEVLRPTDLTEVDRDYVAGLLCIR---NMDDDPKLFTALELPFSTSSARGHEVPL--STRYTHISPRNRAEYVRLALGFRLHEFDEQVKAVRDGMSKVIPVPLLSLFSAAELQAMVCGSPDIPLGLLKSVATYKGFDPSSALVTWFWEVMEEFTNQERSLFLRFVWGRTRLPRTIADFRGRDFVLQVLEKNPPDHFLPESYTCFFLLKMPRYSCKAVLLEKLKYAIHFCKSIDTDEYARVAMGEPTEATGSED 4900          

HSP 2 Score: 156.762 bits (395), Expect = 2.568e-36
Identity = 105/347 (30.26%), Postives = 168/347 (48.41%), Query Frame = 0

Query: 2574 GGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD------------YFSLTPVVTLLLSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSP 2866
            GGS S+       F +  +G +YACGEG+ G+LGLG + +            Y      V     H L       VFSWG+GEDGKLGHGN     +P+++ +L       VA G+SHSA ++ +G+++T+G G+ GRLGHG+  T+  P+ +++L G  V  VACG        L      F+W +                           V +I  G  FSL LT +G+V+  G   +  +  +  + +++ P+ I  L    ++ +AVG  H LA++ +G V+ WG N  GQ G+ + +  K+P ++  L    + +++ G +HS AW  P   ++     P
Sbjct: 3026 GGSKSL-------FIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLG-HGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVACGSSHSIAWGLPNASSDEEKRGP 3364          

HSP 3 Score: 151.754 bits (382), Expect = 8.111e-35
Identity = 103/331 (31.12%), Postives = 164/331 (49.55%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKDNHFS-------NVHFPSP-------IIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTM 583
            I  GS   +++  +GKV +C +  +         NV  P         ++            LALT D KV+SWG G  G+LGHG++   + P+ + +L    I  ++ G  H+AAI+  G LYTWG G  GRLGH D      P LV  L    V  V+CG++   TL +++DG  V+S+G G+ G+LG+G +    TP  I    G+ + ++    +FSLAL+ +G++W WGK  +       +     P+ I  +    + +V+ G  H + + +   VY+WG N +G+ G G T +  K P     LD   ++ +  G + S AW +
Sbjct: 3024 IAGGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGA-VFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVN-KKPALVIGLDAVFVNRVACGSSHSIAWGL 3352          

HSP 4 Score: 139.043 bits (349), Expect = 6.042e-31
Identity = 84/244 (34.43%), Postives = 134/244 (54.92%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSS-------TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK 561
            LALT + +VY+WG G  G+LGHG++  Y+ PK +  L    +  I+ G  H+AAIT  G++ TWG+G  GRLGH D      P LV+ L  +    ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+AL+ SG ++ WGK  ++            PK + ++    I +++TG  H +   ++  VY+WG N  G+ G G T   ++ P+
Sbjct: 4149 LALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVDHVRRPKKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDG-TVTAIQRPR 4390          

HSP 5 Score: 119.783 bits (299), Expect = 4.290e-25
Identity = 87/277 (31.41%), Postives = 136/277 (49.10%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW---------NTSSTVPKWINSIS--SISNVSTGGFHLIVILENRSVYSWGLNTYGK-CGLGLTS---LKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKG 588
            LA+  +H  YSWG G   RLGHGD      P +I++L    +  +  G  ++AAIT  G L TWG G+  RLGH +     +PTLV  L  H V  V+ G+   H+L ++ +G+ V+++G G+ G+LG G+      P L+     V  ++V A ++FS+ALS  G L+ WGK                +VP+ ++S+    I +V+    H + +  +  V+ WG N   + C   ++S   L+      L  +   GI     QS  WT    +G
Sbjct:  629 LAIDSNHNAYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGL-VFAWGDGDYGKLGNGNCNGSLQPILVESLPRV--QRVFAGSQFSVALSSEGQLYTWGKATCLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQICPASVSSEPLLRTPILVSLPTFPASGIACTSAQSLVWTQSSHQG 902          

HSP 6 Score: 100.908 bits (250), Expect = 2.034e-19
Identity = 66/211 (31.28%), Postives = 101/211 (47.87%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADL-GSSSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSD--------DYFSLTPVVTL-------LLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
            +  +Y  G G+ G+L     ++ + PK   +  G     V CG +   T AL  +G++++ G+G +GKLG G S+        +  S   VV +       L       V++WG G+  +LGHG  +  R+P+ I  L G     VAVG  H   +TD G+++ +G+ D G+ G GN    K P  +  L   FV  VACG  H+    AW
Sbjct: 3143 QGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVACGSSHS---IAW 3350          

HSP 7 Score: 94.7449 bits (234), Expect = 1.508e-17
Identity = 68/217 (31.34%), Postives = 99/217 (45.62%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES------------------------VTKICLGRTFSLFLTS-GDVFFCGNSKFSGMD-INSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCN 2812
             +SWG GED +LGHG+T     P  I++L  +    V  G S+SA +T  G + T+G G   RLGHGN   +  P  + +L  + V  VA G        L    + FAW +                         V ++  G  FS+ L+S G ++  G +   G   +  S      P+ +  L++  +VD+AV   H LALS SG V+GWG N
Sbjct:  637 AYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEGQLYTWGKATCLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRN 853          

HSP 8 Score: 94.7449 bits (234), Expect = 1.573e-17
Identity = 72/264 (27.27%), Postives = 118/264 (44.70%), Query Frame = 0

Query:  263 DIYTWG------------CDLHSPKIWDDVN--DVEKIETGSSCTYVIHTNGKVSCWKDNHFSNV-------HFPSPIIDASLS--------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVS 491
            ++Y WG                 PK+ + +N   V  I  GS+ +  I  +G V  W    +  +            +++A L            ++  L +T+D  V+SWG+G +G+LG G       P +I SL    ++ +  G   + A+T  G +YTWG+G   RLGH  G V  V  P  V  L    + S++ G+ H +  S  G +V+++G  + G+LG G    +  P+L+    G  + KV   +  +LALS S
Sbjct: 4156 EVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGH--GSVDHVRRPKKVAALQGKKIISIATGSLHCVACSDSG-EVYTWGDNDEGQLGDGTVTAIQRPRLVAALQGKHIVKVTCGSAHTLALSTS 4416          

HSP 9 Score: 92.0485 bits (227), Expect = 8.541e-17
Identity = 78/289 (26.99%), Postives = 136/289 (47.06%), Query Frame = 0

Query:  305 WKDNHF--------SNVHFPSPIIDASLSQ-----DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
            W  NH         S +  P+P    SL +       E  + A+T D K+++ G GS GRLG G    +  P  + SL  +    + +++G  H  A+T +G +Y WGEG  G+LGH + + +  P LV++L+  +V  ++CG+ H+  ++  G  V ++G G  G LG GD+     PKL+    G     +   +  +  L ++ D  +W WG   +            +P  I S++ +    V  G    + + ++ +VY+WG   + + G G     V+ PK++
Sbjct: 4054 WGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWAIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASG-HVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVD-HVRRPKKV 4340          

HSP 10 Score: 84.7297 bits (208), Expect = 1.577e-14
Identity = 53/177 (29.94%), Postives = 96/177 (54.24%), Query Frame = 0

Query:  330 SFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCI----SAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFG---CGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGK 499
            S+  A+T    + +WG G++ RLGHG+  D + P  + +L   M +     +G  H+ A+T +G ++ WG+G  G+LG+ +      P LV++L  V  V  G+Q ++ +S +G Q++++G   C  +  + +   GC   P+L++      +  V  S    LALS SG+++ WG+
Sbjct:  678 SYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEG-QLYTWGKATCLGHQLVERSVQGC-SVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGR 852          

HSP 11 Score: 82.0333 bits (201), Expect = 9.960e-14
Identity = 65/228 (28.51%), Postives = 112/228 (49.12%), Query Frame = 0

Query:  341 VYSWGNGSHGRLG--HGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            +Y WG+   G+LG   G +     P E  SL   + ++ G     A+T DG L+  G GS GRLG        +PTL+ +L +V     +V+ G +H L ++ +G +V+++G G +G+LG G+      PKL+   +G+ +  +   +  S A++ SG +  WGK  +       +     PK + ++     +     +G    + I ++ +V+SWG   YGK G G
Sbjct: 4051 IYGWGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWAIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEG-EVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRG 4277          

HSP 12 Score: 74.7146 bits (182), Expect = 1.530e-11
Identity = 69/228 (30.26%), Postives = 102/228 (44.74%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLA--DLGSSSIPPKYAD-SFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLV------------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHG----NFQTKKSPEQISSLRGYFVTYVACGLEH------TKINFAW-RESVTKIC 2741
             Y  G G++ +L   D  + ++P K A        SV CG   + A+   G+L   G G+Y +LG GNSDD  SL  +V  L  H +V                   VF+WGDG+ GKLG+GN     +P ++ SL      F   G+  S  L+ +G+++T+G      LGH     + Q    P  +SSL+   +  VA  + H      +   F W R    +IC
Sbjct:  637 AYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDR-SLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVF--AGSQFSVALSSEGQLYTWGKAT--CLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQIC 859          

HSP 13 Score: 71.2478 bits (173), Expect = 2.035e-10
Identity = 55/217 (25.35%), Postives = 94/217 (43.32%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAG 2845
            V  WG  +  +LG     + + P    ++S      +A G+    +++  GK++  G G  GRLG G       P Q+  LR Y V  VA    H+               G   +L LT  G VF  G  +  G   + +   +  P+ +  L  + + D+A G  H  A+S  G ++ WG    G+LG+       +P ++  L+G+ ++Q++ G
Sbjct: 2988 VMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAV---HS---------------GGKHALALTLDGKVFSWGEGE-DGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACG 3185          

HSP 14 Score: 70.4774 bits (171), Expect = 3.120e-10
Identity = 63/236 (26.69%), Postives = 105/236 (44.49%), Query Frame = 0

Query:  375 ISAGHHHAAAIT---LDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSV--SCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIW-----GKTPWNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG--LTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            +S  H  AAA     L   +  WG     +LG   G    VPT  + +  +  +  + G++   IVS+DG +V++ G G NG LG G T  +  P  +      ++KKV   +  + +LAL++ G ++ W     GK      +T+  P+ + ++ +  I +V+ G  H   I     +Y+WGL  YG+ G G   T LK K    L    +  +  G   +    + +    F W
Sbjct: 2969 VSTSHEEAAAAPEQDLPCTVMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLFIVSQDG-KVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSW 3203          

HSP 15 Score: 62.7734 bits (151), Expect = 6.529e-8
Identity = 45/140 (32.14%), Postives = 74/140 (52.86%), Query Frame = 0

Query:  319 IIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHF------VPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNN 450
            ++D +L    ++  LALT +  V++WG+G +G+LG+G+      P  + SLP +  + AG   + A++ +G LYTWG+ +   LGH+  +V        VP LV +L +  +  V+    H L +S  G     FG G N
Sbjct:  722 VVDVALG-SGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEGQLYTWGKATC--LGHQ--LVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGE---VFGWGRN 853          

HSP 16 Score: 62.003 bits (149), Expect = 1.097e-7
Identity = 50/192 (26.04%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 2667 FVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVG-GD-HVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            F+A+ ++H+A        +++G G+  RLGHG+   +  P +I++L  + V  V CG  ++                   +     G++   G   ++ +   +S+ +   P  +  L +  +VD+A+G GD H LAL+  G V+ WG    G+LGN +     +PI++E L    + ++ AG   S A +S
Sbjct:  628 FLAIDSNHNA--------YSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYS-------------------AAITCGGNLLTWGRGTYARLGHGNSD-DRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESL--PRVQRVFAGSQFSVALSS 789          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|109892197|sp|Q3U487.2|HECD3_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HECTD3; AltName: Full=HECT domain-containing protein 3; AltName: Full=HECT-type E3 ubiquitin transferase HECTD3)

HSP 1 Score: 159.844 bits (403), Expect = 5.356e-38
Identity = 108/372 (29.03%), Postives = 173/372 (46.51%), Query Frame = 0

Query: 2982 PIGSSSIFSQIAEQVF----YIDPKELRLPYRS---WKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTP------NGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTW-KDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMD 3339
            P   +++F+Q+ E +     Y  P + R P R    W+ K + E   D GG F D++++M +EL     +   P P      N  +  G  +D Y+ N    S  D   + ++G L G ALR ++ + + L   VWK +   + +W KD   VD    + L  V    + +  E        F  +    + V L PG   I + YE++  +++     R +E   QV A++ GL  + P  +L LL  Q +E  VCG+    +  L+K+ R+ D +PS+  + + WE L  F N+ +  FL FV+GRSRLP   +    +          + LP + TC   L LP Y S ++   +L YA  +C  ID D
Sbjct:  492 PACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNP---SCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLE-VMEGVDKETFEFKFGKELTFTTVLSDQQVVELIPGGTGIVVEYEDRSRFIQLVRKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDTRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGY----ETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTD 855          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|74744877|sp|Q5T447.1|HECD3_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HECTD3; AltName: Full=HECT domain-containing protein 3; AltName: Full=HECT-type E3 ubiquitin transferase HECTD3)

HSP 1 Score: 157.532 bits (397), Expect = 2.926e-37
Identity = 107/372 (28.76%), Postives = 172/372 (46.24%), Query Frame = 0

Query: 2982 PIGSSSIFSQIAEQVF----YIDPKELRLPYRS---WKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTP------NGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTW-KDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMD 3339
            P   +++F+Q+ E +     Y  P + R P R    W+ K + E   D GG F D++++M +EL     +   P P      N  +  G  +D Y+ N    S  D   + ++G L G ALR ++ + + L   VWK +   + +W KD   VD    + L  V    + +  E        F  +    + V L PG   I + Y ++  +++     R +E   QV A++ GL  + P  +L LL  Q +E  VCG+    +  L+K+ R+ D +PS+  + + WE L  F N+ +  FL FV+GRSRLP   +    +          + LP + TC   L LP Y S ++   +L YA  +C  ID D
Sbjct:  492 PACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPLPFFVRTANQGNGTGEARDMYVPNP---SCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGEEVSWSKDFPAVDSVLVKLLE-VMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGY----ETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTD 855          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|75171768|sp|Q9FN03.1|UVR8_ARATH (RecName: Full=Ultraviolet-B receptor UVR8; AltName: Full=Protein UV-B RESISTANCE 8; AltName: Full=RCC1 domain-containing protein UVR8)

HSP 1 Score: 129.028 bits (323), Expect = 8.919e-30
Identity = 81/236 (34.32%), Postives = 124/236 (52.54%), Query Frame = 0

Query:  330 SFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGY-LYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            S  +AL     V SWG G  G+LGHGD +D   P ++S+L    I+ ++ G  H  A +  G  +Y+WG G  GRLGH +    F P  +K LH   +  ++CG  H L V+ +G +V S+G   NG+LG GDT     P+ I  F+G+ +K V A    + A++  GDL+ WG   +        T   VP+ + S     +S V+ G  H I +  + ++Y++G + YG+ G G
Sbjct:   25 SHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEG-EVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRTDRLVPERVTSTGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLGHG 259          

HSP 2 Score: 124.79 bits (312), Expect = 2.315e-28
Identity = 97/325 (29.85%), Postives = 152/325 (46.77%), Query Frame = 0

Query: 2550 GNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNG-SLYACGEGSYGKLGLGNSDDYFSLTPVVTLL----------LSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            G G++GQL    +   P P    +  G    SV CG   T A   +G  +Y+ G G +G+LG GNS D F+  P+  L            SH L       V SWG  ++G+LG G+TE    P+ I +  G     VA G  H+A +T+ G ++ +G G  G LG G+   +  PE+++S  G  ++ VACG  HT I+ ++                  SG ++  G SK+  +       ++  P K+  L N  +  I+ G  H +AL+  G ++GWG N  GQ+G  +      P+ +     + ++Q+S G  H+ A T
Sbjct:   40 GRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRTDRLVPERVTSTGGEKMSMVACGWRHT-ISVSY------------------SGALYTYGWSKYGQLGHGDLEDHL-IPHKLEALSNSFISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVT 344          

HSP 3 Score: 119.783 bits (299), Expect = 1.244e-26
Identity = 80/258 (31.01%), Postives = 123/258 (47.67%), Query Frame = 0

Query:  329 ESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMC--ISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNT------------SSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDG 570
            +S  LA+T + +V SWG   +G+LG GD +D   P++I +   I    ++AG  H AA+T DG LY WG G  G LG  D     VP  V +     +  V+CG +HT+ VS  G  ++++G    G+LG GD      P  +       + ++    R ++AL+  G L+ WG   WN              S V         +  VS G  H + + E  +V++WG  T G+ G+G  S+    PK ++   +DG
Sbjct:  129 DSHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRTDRLVPERVTSTGGEKMSMVACGWRHTISVSYSG-ALYTYGWSKYGQLGHGDLEDHLIPHKLEALSNSFISQISGGWRHTMALTSDGKLYGWG---WNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGIG-ESVDRNFPKIIEALSVDG 381          

HSP 4 Score: 115.931 bits (289), Expect = 2.091e-25
Identity = 66/195 (33.85%), Postives = 102/195 (52.31%), Query Frame = 0

Query:  370 PP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            PP  ++ ISAG  H+ A+     + +WG G  G+LGH D      PT +  L  H + SV+CGA HT+  S+ GM+V+S+G G+ G LG G++  L+TP  I    G+ +K++   +   LA+++ G++  WG+             S VP+ I +     I  V+ G  H   + E+  +Y WG   YG  GLG
Sbjct:   13 PPRKVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLG 207          

HSP 5 Score: 100.908 bits (250), Expect = 1.690e-20
Identity = 78/274 (28.47%), Postives = 129/274 (47.08%), Query Frame = 0

Query: 2599 GEGSYGKLGLGNSDDYFSLTPVV----------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            G G  G+LG G+++D  S T +                 T+  S + + V+SWG G+ G+LGHGN+     P  I +L G     +A G SH   +T +G++ ++G    G+LG G+ +    P++I +  G  +  VA G EHT        +VT+             GD++  G  ++  + +      +  P+++     E M  +A G  H +++S SG ++ +G +  GQLG+     +  P  +E LS   I QIS G  H+ A TS
Sbjct:   40 GRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTA-------AVTE------------DGDLYGWGWGRYGNLGLGDRTDRL-VPERVTSTGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLGHGDLEDHLIPHKLEALSNSFISQISGGWRHTMALTS 293          

HSP 6 Score: 82.8037 bits (203), Expect = 1.469e-14
Identity = 80/262 (30.53%), Postives = 121/262 (46.18%), Query Frame = 0

Query: 2550 GNGQNGQLADLGSS--SIPPKYADSFGGS--SSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP------------VVTLLLSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT-----KIN-FAW-RESVTKICLG----RTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPK 2779
            G  QNGQL  LG +  S+ P+   +F G     V  G + T A+  +G LY  G G YG LGLG+  D   L P            +V     H +       ++++G  + G+LGHG+ E    P  + +LS +F + ++ G  H+  LT  GK++ +G    G++G GN   + SP Q+       V  V+CG  HT     + N FAW R +  ++ +G    R F   + +  V        SG  I SSN++  S K
Sbjct:  145 GRNQNGQLG-LGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRTD--RLVPERVTSTGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLGHGDLEDHLIPHKLEALSNSFISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGIGESVDRNFPKIIEALSV-----DGASGQHIESSNIDPSSGK 398          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|378548419|sp|F8W2M1.2|HACE1_DANRE (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1)

HSP 1 Score: 131.339 bits (329), Expect = 4.586e-29
Identity = 88/291 (30.24%), Postives = 142/291 (48.80%), Query Frame = 0

Query: 3061 DKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-------LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRD-MDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVS------QRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHC 3333
            D      + NS V+  H   F F G + G+AL  R+ + I      +K IL I  +++D+  +D  Y+++L ++      DN+   L       + TD F  +     EVPL PG   I+++ +NK+EYV+     R  + + PQ+ A  +G     P  +++L     +E L+ G     +   K+   Y    D    VI W WE++E    + ++L L FV+G SR+P   F         Q+F +  V    N LPT+ TC   L+LP Y SK+++R+RL+ A+ HC
Sbjct:  616 DGTTFQPNSNSSVNPDHLNYFRFAGQILGLALYHRQLVNIYFTRSFYKHILGIPVSYQDVSSIDPEYAKNLQWIL-----DNDISDLGLELTFSVETDVFGTM----EEVPLKPGGTTIQVTQDNKEEYVQLVTELRMTRAIQPQINAFLQGFHTFIPPSLIQLFDEYELELLLSGMPEIDVMDWKRNTEYTSGYDLQEPVIQWFWEVVENLTQEERVLLLQFVTGSSRVPHGGFAFLMGGSGLQKFTVAAVPYTSNLLPTSSTCINMLKLPEYPSKDVLRDRLLVAL-HC 896          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|123915887|sp|Q28BK1.1|HACE1_XENTR (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1)

HSP 1 Score: 130.568 bits (327), Expect = 9.161e-29
Identity = 106/374 (28.34%), Postives = 172/374 (45.99%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLN----LLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-------LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPL--WILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVS------QRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHC 3333
            +   SIF    E VF  + ++L+   +   V+  GE      G+    + E    LS +++N    L T + +G +             + NS V+  H   F F G + G+AL  R+ + I      +K IL I   ++D+  +D  Y+++L ++      DN+   L       + TD F  +     EVPL PG   I ++ ENK EYV+     R  + + PQ+    +G     P  +++L     +E L+ G     +  W +K        +  ++VI W WE+++E   + ++L L FV+G SR+P   F         Q F I  V    N LPT+ TC   L+LP Y SKEI+++RL+ A+ HC
Sbjct:  560 VHRDSIFRSSCEVVFKSNCEKLK---QGIAVRFHGEE-----GMGQGVVREWFDILSSEIINPDYALFTQSADGTT----------FQPNSNSSVNPDHLNYFRFAGEILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWIL-----DNDISDLGLELTFSVETDVFGAM----EEVPLKPGGASILVTQENKAEYVQLVTELRMTRAIQPQINGFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDW-MKNTEYTSGYERDDQVIQWFWEVVQELTQEERVLLLQFVTGSSRVPHGGFAYIMGGSGLQNFTIAAVAYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVAL-HC 904          

BLAST of EMLSAG00000002245 vs. SwissProt
Match: gi|82200059|sp|Q6DCL5.1|HACE1_XENLA (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1)

HSP 1 Score: 130.183 bits (326), Expect = 1.311e-28
Identity = 109/373 (29.22%), Postives = 172/373 (46.11%), Query Frame = 0

Query: 2983 IGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGV---FDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-------LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPL--WILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVS------QRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHC 3333
            +   SIF    E VF  + ++L+   +   V+  GE     G V   FD   SEM   ++ D   L T + +G +             + NS V+  H   F F G + G+AL  R+ + I      +K IL I   ++D+  +D  Y+++L ++      DN+   L       + TD F  +     EVPL PG   I ++ ENK EYV+     R  + + PQ+    +G     P  +++L     +E L+ G     +  W +K        +  ++VI W WE+++E   + ++L L FV+G SR+P   F         Q F I  V    N LPT+ TC   L+LP Y SKEI+++RL+ A+ HC
Sbjct:  592 VHRDSIFRSSCEVVFKSNCEKLK---QGIAVRFHGEEGMGQGVVREWFDILSSEM---INPDYA-LFTQSADGTT----------FQPNSNSSVNPDHLNYFRFAGEILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWIL-----DNDISDLGLELTFSVETDVFGAM----EEVPLKPGGASILVTQENKAEYVQLVTELRMTRAIQPQINGFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDW-MKNTEYTSGYERDDQVIQWFWEVVQELTQEERVLLLQFVTGSSRVPHGGFAYIMGGSGLQNFTIAAVAYTANLLPTSSTCINMLKLPEYPSKEILKDRLLVAL-HC 936          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: gb|EEZ98478.1| (putative E3 ubiquitin-protein ligase HERC1-like Protein [Tribolium castaneum])

HSP 1 Score: 761.525 bits (1965), Expect = 0.000e+0
Identity = 467/1269 (36.80%), Postives = 699/1269 (55.08%), Query Frame = 0

Query: 2167 LATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGNI-IYIWDMEDSEEN-CNQIPQLSPIIYLCWSP---NSEIEILLAARQNGTSISITTLSWS-SNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGIT--CLHWINSKTLAVGFCNTENVTLMNLPN---EMDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLC-------------SLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRH-NIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS-----IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVA-TSFNPDNEED--SLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE 3348
             A    DGT++L   +E   I        +TL+        L WSP G  L     ++   +W ++DS+    + + Q    + L WSP   +S+  +LL     GTS   T  +W   ++D+  I  +    LV+Q          +    V+ L  HK+   +LASG    P GI+N+WS++  S+++T++G GG+    L W +  +LAV F  ++++ L+            +  ++C A + + +   + A +F+  I      I++QY  EKL +  G QL +S +L++L+ LA +L+L  ++C              +P+   +WL +FT        L KR    +  L ++    E      +++  WT + D  +M+W    P DWQ GGK   Y+ G+ ++GQLA+LG S+  P   +SF  +  +ICG   TF +Q +G++ ACGEGSYG+LG GNSDD  SL+        V+T L +      H+L       VFSWGDG+ GKLGHGN +R RRP+ I +L       VA G  HSAV+T  GK+FTFGNGD G+LG G+   KK PE++ +L GY +  VACGL HT                                 K+       + K+  G   +LFLT  G VF CG  +     +      +  P+ +  L +  + D+AVG +HVL L+  G V GWG    GQLG       + P II ELS KGI QIS G+ HSAAW++PP+P       P   R    G P ++P ++   L   S      R+  LH  S+ + + W L P+   +    P ++     + + ++R  L+  V T P ++ +  TM      +NYGPQ+ V+R+     +      +F QIA+QV  I P+ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQE++  ++ LL PTPN +++ G N+D+YLLN   N++  +  F FLG+L GVA+RTRKP+AI LAP++WKLI+    T +DLE+VD  Y +SL  +     +   EE+   ++P + F G S +G+ +P+  G   I L++ N+ +Y + A+  R +E   Q+ ++R+G++ + P P+L L+ +  +E LVCG +   + ILKKI RYR++D ++ ++ WLW ILE F++  ++LF+ FVSGRSRLP +  D+SQRFQ+++V++  NGLPTAQTCFFQLRLPPYTS+EIM  RL YAI +C++IDMD+YML RN +
Sbjct: 2957 FAVAYLDGTLKLGWIDENTNI--------TTLKAHESGISSLEWSPDGSILATISSDLNCKLWHLKDSKLTLIHTLSQTHEPVTLKWSPLIGDSKTPLLLTI---GTSYG-TVCAWRLPDTDHNNIKPQ----LVMQAQG-------HACNAVTGLAVHKNG-LLLASGC---PNGILNVWSLVDGSLVQTVVGSGGVNYNGLLW-SGDSLAVAFLRSKSIGLLQYGTGSLTKYQSLTTARC-ALIRKGVKGLKSAQFFKTLILYLPKIILDQYNIEKLAVQTGAQLSHSSYLKSLTSLAILLDLEHIICYKCRPFNNKNESDVIPE--MQWLHTFTLGAQIADCLIKRTNLPECVLSQARGVAECVTTEAVQNASWTSKQDEQIMQWVSQRPQDWQIGGKCKAYMWGSDRHGQLAELGYSASVPVQVESFSIARKIICGQNCTFVIQSDGTVLACGEGSYGRLGQGNSDDLHSLSVISSLQGFVITDLATSVGSDGHSLALAESGEVFSWGDGDFGKLGHGNPDRQRRPRQIEALQTEQVVQVACGFKHSAVVTSDGKLFTFGNGDFGKLGLGSVANKKLPERVMALEGYRIGQVACGLNHTACVSTDGMTVWTFGEGDYGKLGLGYATMKLLPQKVETVCNLGIKKVGCGTHLTLFLTKDGKVFVCGIDRMPWHSLFRERA-VFKPQIMTSLSDYSIEDLAVGTEHVLFLTTCGKVLGWGMTGEGQLGLPHVSLVRHPEIITELSDKGIRQISTGRTHSAAWSAPPLPQRV----PGVTRSLTFGLPANIPSQY-DHLQGLSIELIQARVMFLHRFSDMLYSCWTLMPLSHQEGMKLPPLE----GLVSPSLRPLLASRVYTFPFVRCIGKTMVQG---KNYGPQIIVKRINQEDVTGRKCKPVFVQIAKQVVDIKPQELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQEITSGIVPLLVPTPNAVNEDGFNRDRYLLNPQLNTQQHIAWFKFLGILFGVAIRTRKPLAIPLAPMMWKLIVGEVVTIEDLEEVDCMYVQSLRSIRDIHLSGVGEENFHEVIPLECFEGTSCTGKVLPIVYGGRSIPLTFHNRTQYFERAVKFRLEEFDLQITSIREGMAGIVPVPLLSLMTADYLEQLVCGMTHISIPILKKIIRYRELDENHNLVRWLWNILEGFSDAERVLFMRFVSGRSRLPANLADLSQRFQVMKVDKAVNGLPTAQTCFFQLRLPPYTSQEIMAERLRYAINNCKSIDMDNYMLARNTD 4181          

HSP 2 Score: 331.257 bits (848), Expect = 1.662e-89
Identity = 258/793 (32.53%), Postives = 394/793 (49.68%), Query Frame = 0

Query:   39 AQQIRTSSYFQNRLIFKLQGLENKECAEVENKDDILKLDLQRSESFSNPEEDTCRSESLQ-NEALLNLGLQTGLSLLFSLLQQNWSGPD--EQHPLSSTVISTANNMLLSFPPLSL---LAVPPLGETALDNVATFLHTSA-QIPQAST----SCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAVQLAQGR---------TYDSHTIYLKEAAMYLTEQI-------------VHFTSRN------KQDIYTWG-------CDLHSPKIWDDV-----NDVEKIETGSSCTYVIHTNGKVS-CWKDNH-------FSNVHFPSPII------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIW------GKTPWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTMRKGKG---CFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFD-EDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKMNNLSKGQQYKLKMDLESLNEPGIISGLICESEE 747
            A+ + +SS F   L  +L  L     A +    D  K D   SE+ +N        ESL  ++ALL +G++TGLSLLFSLL+QNW   D  +   L ++V+ T+ ++L   PPL L     +  LG T+L+ V+ FL  +    P A +       +LLL+LA+QRG    +L WI   L  + +   +  +    +  +QL  G+            S  + + EAA+ L E +             V  TS +      K D+Y WG        + +  KI   V       V+++E G  CT+ IH +G VS C K ++        SN   P  I+        S S+ ++   LAL E   VYSWG+G +G+LGHG+   +  P+ I+       I  I AG+ H+AA+T DG LYTWGEG  GRLGH D    +VPT V  L  VGSV+CG+ HTL+VSKDG  VWSFG G +G+LG G+ G +Y P+++    G+ ++KV A   FS+AL+ +G+++ W      GK   +    +P ++  ++   I ++S G  H + + +   V++WG N+ G+CG G T   +  P +   L+   I  I AG + S AWT    +      H P+  +   KTF  +   L  + +S         F    E  +F+L    LL ++    I+ ++     S    +L  I+F+ +D +   PEIY  VI+ I VGA  L PQL++RV   ++ L  + L++GQQ  L + L SL +P  I+ L+   E+
Sbjct:   90 ARNVCSSSQFAAILKQRLLILRRIYHALIVKYHDKAKTDQTTSENNTN----ILAKESLSGSQALLEIGVKTGLSLLFSLLRQNWQVSDILKVPSLCNSVLETSFDLLQKLPPLCLSNDTQLTNLGITSLEQVSDFLKDAVLNSPNADSHAKLLSCKLLLALALQRGSLRYLLDWIEMVLDASCKTEGILCDSFFKSAIMQLESGKYKIKSDLWKGQTSSEVSVYEAAINLMEILAGMAIDFGGVCSAVETTSESEVGVYEKSDVYVWGSNSSHQLAEGNQEKILMPVKSKMFTQVQQVEAGQYCTFAIHWDGSVSACGKGSYGRLGLGESSNQSLPKRILLDCVVKKLSSSKGSDGHTLALAETGVVYSWGDGDYGKLGHGNCATHKQPERITGPFLGKTIKYIHAGYRHSAAVTDDGKLYTWGEGDHGRLGHGDSNARYVPTQVAGLAEVGSVACGSSHTLVVSKDGKTVWSFGSGEHGKLGTGELGKVYRPQVVEALQGLTIQKVCAGTWFSMALTTAGEVYTWGSGAILGKGSADAIVNLPMYVEDLAQYRIVDISAGDNHCLALTDEHEVFAWGTNSMGQCGQGHTCSPITRPLKVIGLEGVSIRQISAGTSHSMAWTSMSTENHHMTKHKPFCLDLHEKTFILLKKFLEKYTVSFIYETPPPPFKTATEHHRFVLLTLKLLCTHLSLCINGNLSDNILSNHTKQLRLILFRLVDIKT-PPEIYSTVIELINVGASLLLPQLQERVEFVHEHLSKDGLTQGQQMLLNIVLCSLEDPAHIAALLRIQEQ 877          

HSP 3 Score: 175.637 bits (444), Expect = 2.152e-42
Identity = 131/382 (34.29%), Postives = 184/382 (48.17%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDY-----FSLTPVVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRP-KIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI---------NFAWRE------------------------SVTKICLGRTFSLFL-TSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGN-ESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKP 2869
            KS VY+ G+  + QLA+     I  P  +  F     V  G   TFA+  +GS+ ACG+GSYG+LGLG S +        L  VV  L S      H L       V+SWGDG+ GKLGHGN    ++P +I     G    ++  G  HSA +TD GK++T+G GD GRLGHG+   +  P Q++ L    V  VACG  HT +         +F   E                        ++ K+C G  FS+ L T+G+V+  G+    G    S++  +  P  +  L    +VDI+ G +H LAL+    V+ WG N +GQ G   +      P+ +  L G  I QISAG +HS AWTS  M T  ++ +  KP
Sbjct:  347 KSDVYVWGSNSSHQLAEGNQEKILMPVKSKMFTQVQQVEAGQYCTFAIHWDGSVSACGKGSYGRLGLGESSNQSLPKRILLDCVVKKLSSSKGSDGHTLALAETGVVYSWGDGDYGKLGHGNCATHKQPERITGPFLGKTIKYIHAGYRHSAAVTDDGKLYTWGEGDHGRLGHGDSNARYVPTQVAGLAE--VGSVACGSSHTLVVSKDGKTVWSFGSGEHGKLGTGELGKVYRPQVVEALQGLTIQKVCAGTWFSMALTTAGEVYTWGSGAILGK--GSADAIVNLPMYVEDLAQYRIVDISAGDNHCLALTDEHEVFAWGTNSMGQCGQGHTCSPITRPLKVIGLEGVSIRQISAGTSHSMAWTS--MSTENHHMTKHKP 722          

HSP 4 Score: 157.918 bits (398), Expect = 4.724e-37
Identity = 114/383 (29.77%), Postives = 182/383 (47.52%), Query Frame = 0

Query:  251 EQIVHFTSRNKQD--------IYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI--------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWG--KTPWNT-----SSTVPKWINSIS--SISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWT 582
            EQI+ + S+  QD         Y WG D H            P   +  +   KI  G +CT+VI ++G V    +  +         ++H  S I         D + S  ++   LAL E  +V+SWG+G  G+LGHG+      P++I +L    ++ ++ G  H+A +T DG L+T+G G  G+LG        +P  V  L  + +G V+CG  HT  VS DGM VW+FG G+ G+LG G       P+ +     + +KKV      +L L+  G +++ G  + PW++     +   P+ + S+S  SI +++ G  H++ +     V  WG+   G+ GL   SL V++P+    L    I  I  G T S AW+
Sbjct: 3308 EQIMQWVSQRPQDWQIGGKCKAYMWGSDRHGQLAELGYSASVPVQVESFSIARKIICGQNCTFVIQSDGTVLACGEGSYGRLGQGNSDDLHSLSVISSLQGFVITDLATSVGSDGHSLALAESGEVFSWGDGDFGKLGHGNPDRQRRPRQIEALQTEQVVQVACGFKHSAVVTSDGKLFTFGNGDFGKLGLGSVANKKLPERVMALEGYRIGQVACGLNHTACVSTDGMTVWTFGEGDYGKLGLGYATMKLLPQKVETVCNLGIKKVGCGTHLTLFLTKDGKVFVCGIDRMPWHSLFRERAVFKPQIMTSLSDYSIEDLAVGTEHVLFLTTCGKVLGWGMTGEGQLGLPHVSL-VRHPEIITELSDKGIRQISTGRTHSAAWS 3689          

HSP 5 Score: 124.79 bits (312), Expect = 5.681e-27
Identity = 144/614 (23.45%), Postives = 268/614 (43.65%), Query Frame = 0

Query: 1157 DEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLLECLTGC--------------PSILELSIVR-CLGKILSHFEIGRNREHIVRKLFSHIYNSYWNSKSSDLDFTNIQRTS-----------------YNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINN---RSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXF---------SSLISQSEKLRGSIDNVLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEE-----RNKNKENPASTS-----------------QAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTL-HEVF-------------FKVIALQFSSIKAM 1686
            D+   D + F+ V+ SS    V+ + L+  K   W+  LL  L                 P ++ L I++  L  +   F     R+ +V KLF+ +    W   S++   T +  +                  ++M F+++  ++C+++   T+V      GY +  + I  G   WKI I+K+++G +   IGV+   + +   RS  DMWLY + SG L H  G        +++GD I  +L+ +  +LSF KNG    +AF ++ ++ ELYP V F ST    K+ I+++ ++      L   EP+L  L   + +  V +IR L+N  +  W + ++S     ++     ++DL +           S   S S  +   I+ +   ++P L  +   D GL +G Y ++K   +R I++G+       V ++WE DGS S  S+  L+ I+   +P +       S  L+  +  +   + +L    ++  ++EEE     R+   ++  S S                 + ++++TD +VS+I+ E    S         E  I K+    T+ HE+               ++  LQF+++K +
Sbjct: 1543 DKSFGDFLVFLRVIGSS---KVIKTVLASKK---WLEALLSVLDTSDLFMSYPVQLKLLRPKMMVLQILQNILPGLQPKFLPSDLRKSVVNKLFTQLGKELWKKTSTESLPTQLVESELKLNDLLDEDEEENIPVHDMGFDINKCHNCVIESNLTLVHGFGGRGYGLGLQAIRSGCYQWKILIVKENRGNEGTCIGVSKFPVRDFSHRSTTDMWLYRAYSGSLYHS-GERDISFQCYTQGDYITVVLDMDAKTLSFGKNGEEPRVAFENIEAT-ELYPCVMFYSTGPGEKVKITDM-KVHSKQRDLLPGEPNLAPLPAVLTEAYVTLIRRLHNADA--WTNEVNSA----IIDRLSRIEDLFEQIHFYNSDGEESEENSNSRDMYSKINELCLTVWPALVTIGGFDRGLRIGGYCKHKGTGRRAIVLGILKKGITTVNVQWESDGSISDVSMSNLEHIET--APFNNNKFSGLSPSLLFQVARLTGLTNELVSPEYNLTRTEEELLCEDRHCRSDSLRSCSDSQIHNQYTFLDTPAGPRTIDSLTDEMVSTILVESKRISVEKMVGTQSEATISKTPEKKTINHEIKKLEKKLLDLEANCLQIAFLQFAAMKTL 2139          

HSP 6 Score: 62.3882 bits (150), Expect = 3.930e-8
Identity = 69/269 (25.65%), Postives = 117/269 (43.49%), Query Frame = 0

Query: 2066 PQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIG--FHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSY--NDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQI-----------LTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPII---YLCWSPNSEIEILLAARQNGTSISITTLSW-------SSNSDYLAIGYENGEI-LVLQID---QRKESI 2305
            P+   ++ L D   + P  +++ALS+ +LS R+   +     + WA++ + K I     + +       N +D+   LP++   Y   GH +++ ++  +  N+ LA+   DGT+RLW   E +  L +D    +TL   + SD             + WSP G+YL  ++ N+I IW                PII   Y  W    + E             ITTL+W         + D+L +G  +G + LV+  D   QR ES+
Sbjct: 2707 PKDEVASPLSDPNTLEPLTLVNALSAYVLSFRVDQEN-----REWASEQLFKTIANKIQILSGSTSEQTNFADLTNFLPKERMEY-FDGHDNRVSTLAYHEKNNKLASAGYDGTVRLWV-FESRTQLSLD----ATLVFHISSDIFGNELQGRLIGHVKWSPRGEYLAAAMDNVINIW----------------PIINEGYQNWFIEDQKEF------------ITTLTWPKFKLNEEKDRDFLLVGKIDGSVSLVIMSDGGIQRVESL 2936          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: XP_016772734.1 (PREDICTED: E3 ubiquitin-protein ligase HERC2 [Apis mellifera])

HSP 1 Score: 457.988 bits (1177), Expect = 7.000e-128
Identity = 311/1005 (30.95%), Postives = 487/1005 (48.46%), Query Frame = 0

Query: 2425 IIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFI-SLLNKRNAKSDHELLKSLFFDEI--KKDVNLKDI----VWTPEADSHLMKWYIVYPSDWQPG--GKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPL----VATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            +++QY YE   +  G QL++S F + L  LA  L L+ + C     +W W   +  S+    SL+N+ +      L  +   +E+   K+ N KD     ++  E D  L++W    P DW     G  ++Y  G+   GQL  L  + +  P   +S      V    G Q  FA+  +G +YA G G+ G+LG+G +D    + P +   + H  V                    V+SWG+G+DGKLGHGN     RPK+I  L G     +A G  HSA +T  G ++T+G G  GRLGHG+ + + +P+ I +L+ Y V  VACG    +          ++W +                           V K+  G  FS+ LT SG ++  G   +  +  + ++ +++ P+K+  L+   ++ IA G  H +A +  G V+ WG N  GQLG+ +    + P ++  L GK I +++ G  H+ AW++  M    +    T P         +  + V  LP         RL LLH  +  +     ++P+               +++S       L+P+    +      + + +TM      R +GP + + R+             P G  S+F Q+  ++  +    L  P+R WKVK +GE+ DD GG + ++I+EMC EL    L LL PTPNG  D G+N+D +LLN   +S + M  F FLG+L G+A+RT  P+++NLA  VWK +  I  T  DL ++D  Y   L  +    +PD +    L    F   S  G +V L   S   +++ ENK EYV+ ALN R  E   QV AVR+G+S + P P L L     +E++VCG    PL +LK +A Y+ ++ +  +I W WE++EEF+N  + LFL FV GR+RLP    D   R  +L+V      P++ LP + TCFF L++P Y+ K ++R +L YAI  C++ID D Y
Sbjct: 4055 LLKQYTYEDPLVRGGKQLMHSDFFKVLVALACDLELDGMQCCSEIHKWSWFRRYCLSVRVAKSLINRTSLPKPFCLEVTKQINEMITDKEGNTKDHENHELFKQEHDEQLLQWLNRRPEDWMLSWDGSGAIYGWGHNHRGQLGGLEGAKVKLPVLCESLSALRPVQLTGGEQTLFAVTADGKVYATGYGAVGRLGIGGTDSV--MVPTLLESIQHVFVKKVAVNSGGKHCLALSSEGHVYSWGEGDDGKLGHGNRLSYDRPKLIEELLGTEIVDIACGGHHSAAITSAGWLYTWGKGRYGRLGHGDSEDQLTPKLIEALQDYKVIDVACGSGDAQTLCVTDDDNVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVIKVECGSQFSVALTRSGAIYTWGKGDYHRLG-HGTDDHVRRPRKVAALQGKKIISIATGSLHCVACTDKGEVFTWGDNDEGQLGDGTTSALQRPRLVHALQGKKITRVACGSAHTLAWST--MKATQSKMPATTP---------MEYDLVKDLPFPVLH---NRLVLLHHFAELLCPCLPMYPITGP------------VSLSK------LAPILVYSIKEATFRKVVQATMVRD---RQHGPVIELNRIQVKRARSKGGLAGPDGIKSVFGQMVSKISLLTQDVLFFPHRIWKVKFIGESVDDCGGGYSESIAEMCDELQNGSLPLLIPTPNGRDDNGTNRDCFLLNPIADSPLHMNMFRFLGILMGIAIRTGSPLSLNLAEPVWKQLAGISLTPADLTEIDRDYVPGLLCI-RDMDPDEKMFQTLEMP-FSTPSAIGHDVSL--SSRYRKITPENKHEYVQLALNYRLHEFDTQVSAVREGMSKVIPVPFLALFSGPELETMVCGSPDIPLTLLKSVATYKGIEATAPLIQWFWEVMEEFSNQERSLFLRFVWGRTRLPRTIADFRGRDFVLQVMDKYNPPDHFLPESYTCFFLLKMPRYSCKAVLRQKLKYAIHFCKSIDTDEY 5017          

HSP 2 Score: 171.785 bits (434), Expect = 3.412e-41
Identity = 122/388 (31.44%), Postives = 183/388 (47.16%), Query Frame = 0

Query: 2536 SDWQPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLL------------SHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSP 2866
            S+ QP   S+VY+ G     QL  L  S I  P Y++       V    G +  F +   G LYACGEG+ G+LGLG+  +     P+  L               H L       VFSWG+GEDGKLGHGN+    +P++I SL       +A G+ HSA +T  G+++T+G G+ GRLGHG+  T+  P+ + SL G  V  +ACG        L    + ++W +                           V +I  G  FSL LT  G+V+  G   +  +  + ++ +++ P  +  L  + ++ +AVG  H LA++  G V+ WG N  GQ GN +    K+P ++ EL    + ++S G +HS AW     P   NN  P
Sbjct: 3159 SEPQPQ-HSAVYVWGLNDKDQLGGLKGSKIKLPVYSEVLSKLKPVHIAGGSKTLFVVSQEGKLYACGEGTNGRLGLGDDSNVCEPKPIPFLSQYVIKKVAVHSGGKHALALTQDGKVFSWGEGEDGKLGHGNSISLDKPRLIESLKSKRIRDIACGSGHSAAITSSGELYTWGLGEYGRLGHGDTATQSKPKLVQSLVGQRVIQIACGSRDAQTMALTADGLVYSWGDGDFGKLGRGGSDGCYTPLLIDRLNGLGVVQIECGAQFSLALTKYGEVWTWGKGDYFRLG-HGNDHHVRKPTLVEGLRGKKIIHVAVGALHCLAVTDIGQVYAWGDNDHGQQGNGTTIVNKKPSLVHELDDARVNRVSCGSSHSIAWVLTDQPA-INNQEP 3543          

HSP 3 Score: 166.007 bits (419), Expect = 2.179e-39
Identity = 114/338 (33.73%), Postives = 170/338 (50.30%), Query Frame = 0

Query:  298 TNGKVSCWKDNHFSNVHFPSPI-------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDIL 610
            TNG++    D   SNV  P PI       I            LALT+D KV+SWG G  G+LGHG+    + P+ I SL    I  I+ G  H+AAIT  G LYTWG G  GRLGH D      P LV++L    V  ++CG++   T+ ++ DG+ V+S+G G+ G+LG+G +   YTP LI+  +G+ + ++    +FSLAL+  G++W WGK  +       +     P  +  +    I +V+ G  H + + +   VY+WG N +G+ G G T++  K P     LD   ++ +  G + S AW +         P  N  +  TF    D L
Sbjct: 3227 TNGRLGLGDD---SNVCEPKPIPFLSQYVIKKVAVHSGGKHALALTQDGKVFSWGEGEDGKLGHGNSISLDKPRLIESLKSKRIRDIACGSGHSAAITSSGELYTWGLGEYGRLGHGDTATQSKPKLVQSLVGQRVIQIACGSRDAQTMALTADGL-VYSWGDGDFGKLGRGGSDGCYTPLLIDRLNGLGVVQIECGAQFSLALTKYGEVWTWGKGDYFRLGHGNDHHVRKPTLVEGLRGKKIIHVAVGALHCLAVTDIGQVYAWGDNDHGQQGNG-TTIVNKKPSLVHELDDARVNRVSCGSSHSIAWVLTD------QPAINNQEPVTFPTTKDPL 3553          

HSP 4 Score: 157.147 bits (396), Expect = 1.075e-36
Identity = 112/348 (32.18%), Postives = 182/348 (52.30%), Query Frame = 0

Query:  262 QDIYTWGCDLH----SPKIWDDVND--VEKIETGSSCTYVIHTNGKV-SCWKDNHFSNVH-FPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVH-FVPTLVKNL--HNVGSVSCGA---QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-------WNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAW 581
            Q++  WG  +     SP  +D +++  ++++        ++  NG V   +  +    V+ F    I    S  + +  LALT+D+ VYSWGNG  GRLGHG+   Y+ PK I +L    I  I+ G  ++AA++ +G LYTWG G+ G+LGH +      +PTLV  L  H V  V+CG      TL V+  G+ V+S+G GN+G+LG+G      TPK+++    + + KV    +FS AL+  G+++ WGK                PK I+ + +  I ++  G  H++ + E++ VY WG N +G+    L ++ V  P     +   ++ G+  GPTQS+AW
Sbjct:  436 QEVIAWGSVVTFNGPSPVYYDTISELGIKQLCCTERAVIILSVNGNVYMMYYGSETQRVYGFSEKPITMIASHSDGTHYLALTQDNSVYSWGNGECGRLGHGNMTWYDEPKLIEALVEKNITFIACGSTYSAALSSNGELYTWGRGNYGKLGHGNSDKDVMIPTLVTALNGHMVVYVACGGGRDPQTLCVTASGI-VFSWGDGNHGKLGRGGCDGSKTPKIVDKLLDINVAKVYCGQQFSAALTAYGEVYTWGKGEDYKLGHGNEDHVRYPKIIDILKTKKIKDLYIGHLHVLALTEDQLVYGWGRNDHGQIDPALGTI-VPEPTLCPTFSGKNVIGLACGPTQSFAW 781          

HSP 5 Score: 156.762 bits (395), Expect = 1.121e-36
Identity = 98/272 (36.03%), Postives = 148/272 (54.41%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-----TSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRKG 586
            LAL+ +  VYSWG G  G+LGHG++  Y+ PK I  L    I+ I+ G HH+AAIT  G+LYTWG+G  GRLGH D      P L++ L +  V  V+CG+    TL V+ D   VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+AL+ SG ++ WGK  ++     T   V  P+ + ++    I +++TG  H +   +   V++WG N  G+ G G TS  ++ P+    L    I  +  G   + AW+  K 
Sbjct: 4283 LALSSEGHVYSWGEGDDGKLGHGNRLSYDRPKLIEELLGTEIVDIACGGHHSAAITSAGWLYTWGKGRYGRLGHGDSEDQLTPKLIEALQDYKVIDVACGSGDAQTLCVTDDD-NVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVIKVECGSQFSVALTRSGAIYTWGKGDYHRLGHGTDDHVRRPRKVAALQGKKIISIATGSLHCVACTDKGEVFTWGDNDEGQLGDGTTS-ALQRPRLVHALQGKKITRVACGSAHTLAWSTMKA 4552          

HSP 6 Score: 138.272 bits (347), Expect = 5.511e-31
Identity = 101/339 (29.79%), Postives = 162/339 (47.79%), Query Frame = 0

Query: 2567 PKYADSFG--GSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX----------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKS-PEQISSLRGYFVTYVACG---------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPK--KIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            P Y D+    G   + C  +    L  NG++Y    GS  +   G     FS  P+ T++ SH+             V+SWG+GE G+LGHGN      PK+I +L     TF+A G+++SA L+  G+++T+G G+ G+LGHGN       P  +++L G+ V YVACG         +  + I F+W +                          +V K+  G+ FS  LT+ G+V+  G  +   +   + + +++ PK   I K + + D+ +G  HVLAL++   V+GWG N  GQ+    G    EP +    SGK ++ ++ G   S AW +
Sbjct:  452 PVYYDTISELGIKQLCCTERAVIILSVNGNVYMMYYGSETQRVYG-----FSEKPI-TMIASHSDGTHYLALTQDNSVYSWGNGECGRLGHGNMTWYDEPKLIEALVEKNITFIACGSTYSAALSSNGELYTWGRGNYGKLGHGNSDKDVMIPTLVTALNGHMVVYVACGGGRDPQTLCVTASGIVFSWGDGNHGKLGRGGCDGSKTPKIVDKLLDINVAKVYCGQQFSAALTAYGEVYTWGKGEDYKLGHGNED-HVRYPKIIDILKTKKIKDLYIGHLHVLALTEDQLVYGWGRNDHGQIDPALGTIVPEPTLCPTFSGKNVIGLACGPTQSFAWCT 783          

HSP 7 Score: 102.834 bits (255), Expect = 2.207e-20
Identity = 69/246 (28.05%), Postives = 122/246 (49.59%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFP---KEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            L++  E ++L+      +Y WG+   G+LG  +      P   + +S+L P+  ++ G     A+T DG +Y  G G++GRLG        VPTL++++ +V     +V+ G +H L +S +G  V+S+G G++G+LG G+      PKLI    G  +  +      S A++ +G L+ WGK  +       +     PK I ++     +     +G    + + ++ +V+SWG   YGK G G
Sbjct: 4168 LNRRPEDWMLSWDGSGAIYGWGHNHRGQLGGLEGAKVKLPVLCESLSALRPVQ-LTGGEQTLFAVTADGKVYATGYGAVGRLGIGGTDSVMVPTLLESIQHVFVKKVAVNSGGKHCLALSSEG-HVYSWGEGDDGKLGHGNRLSYDRPKLIEELLGTEIVDIACGGHHSAAITSAGWLYTWGKGRYGRLGHGDSEDQLTPKLIEALQDYKVIDVACGSGDAQTLCVTDDDNVWSWGDGDYGKLGRG 4411          

HSP 8 Score: 102.834 bits (255), Expect = 2.609e-20
Identity = 84/285 (29.47%), Postives = 128/285 (44.91%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLA--DLGSSSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-----------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTS----GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALS 2801
            +Y  G G+ G+L   D  + S  PK   S  G     + CG +   T AL  +G +Y+ G+G +GKLG G SD  +  TP++   L+   V                  V++WG G+  +LGHGN    R+P ++  L G     VAVG  H   +TD G+++ +G+ D G+ G+G     K P  +  L    V  V+CG  H+    AW  +       +    F T+    G +     S  +G++ + SN  I S  K   +   N  DI   GD +L +S
Sbjct: 3326 LYTWGLGEYGRLGHGDTATQS-KPKLVQSLVGQRVIQIACGSRDAQTMALTADGLVYSWGDGDFGKLGRGGSDGCY--TPLLIDRLNGLGVVQIECGAQFSLALTKYGEVWTWGKGDYFRLGHGNDHHVRKPTLVEGLRGKKIIHVAVGALHCLAVTDIGQVYAWGDNDHGQQGNGTTIVNKKPSLVHELDDARVNRVSCGSSHS---IAWVLTDQPAINNQEPVTFPTTKDPLGQMLLTFKSTITGLETDLSNYPIPSCSKTSTVSQINGGDIINNGDIILNMS 3604          

HSP 9 Score: 82.8037 bits (203), Expect = 2.878e-14
Identity = 63/229 (27.51%), Postives = 108/229 (47.16%), Query Frame = 0

Query:  341 VYSWGNGSHGRLGHGDKKDYNFP---KEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGI----VHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLG 551
            VY WG     +LG         P   + +S L P+  I+ G      ++ +G LY  GEG+ GRLG  D         +P L + +    +V  G +H L +++DG +V+S+G G +G+LG G++  L  P+LI       ++ +   +  S A++ SG+L+ WG   +         + + PK + S+    +  ++ G      + +  +  VYSWG   +GK G G
Sbjct: 3168 VYVWGLNDKDQLGGLKGSKIKLPVYSEVLSKLKPVH-IAGGSKTLFVVSQEGKLYACGEGTNGRLGLGDDSNVCEPKPIPFLSQYVIKKVAVHSGGKHALALTQDG-KVFSWGEGEDGKLGHGNSISLDKPRLIESLKSKRIRDIACGSGHSAAITSSGELYTWGLGEYGRLGHGDTATQSKPKLVQSLVGQRVIQIACGSRDAQTMALTADGLVYSWGDGDFGKLGRG 3394          

HSP 10 Score: 73.9442 bits (180), Expect = 1.280e-11
Identity = 55/203 (27.09%), Postives = 89/203 (43.84%), Query Frame = 0

Query:  264 IYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNVHFPS------PIIDASLS-------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTL 433
            +YTWG             D  +PK+ + + D + I+    +G + T  +  +  V  W D  +  +          P+   SL+       +    F +ALT    +Y+WG G + RLGHG       P+++++L    I+ I+ G  H  A T  G ++TWG+   G+LG         P LV  L    +  V+CG+ HTL
Sbjct: 4343 LYTWGKGRYGRLGHGDSEDQLTPKLIEALQDYKVIDVACGSGDAQTLCVTDDDNVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVIKVECGSQFSVALTRSGAIYTWGKGDYHRLGHGTDDHVRRPRKVAALQGKKIISIATGSLHCVACTDKGEVFTWGDNDEGQLGDGTTSALQRPRLVHALQGKKITRVACGSAHTL 4545          

HSP 11 Score: 72.0182 bits (175), Expect = 4.572e-11
Identity = 55/184 (29.89%), Postives = 92/184 (50.00%), Query Frame = 0

Query:  391 LYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSV--SCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTP-------WNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK 561
            +Y WG     +LG   G    +P   + L  +  V  + G++   +VS++G ++++ G G NG LG GD   +  PK I F    ++KKV   +  + +LAL+  G ++ WG+          + S   P+ I S+ S  I +++ G  H   I  +  +Y+WGL  YG+ G G T+ + K PK
Sbjct: 3168 VYVWGLNDKDQLGGLKGSKIKLPVYSEVLSKLKPVHIAGGSKTLFVVSQEG-KLYACGEGTNGRLGLGDDSNVCEPKPIPFLSQYVIKKVAVHSGGKHALALTQDGKVFSWGEGEDGKLGHGNSISLDKPRLIESLKSKRIRDIACGSGHSAAITSSGELYTWGLGEYGRLGHGDTATQSK-PK 3349          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: gb|EEZ97760.2| (hect domain and RLD 2-like protein [Tribolium castaneum])

HSP 1 Score: 454.521 bits (1168), Expect = 5.377e-127
Identity = 324/1035 (31.30%), Postives = 501/1035 (48.41%), Query Frame = 0

Query: 2399 AELFEHLPFEQF-AP--YFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKK---DVNLKDIVWTPEA--------DSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSL-LPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---FQIL-RVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            A+L   L  EQ  AP     + +++    ++ QYEYE + +  G  L++S F + L  LA  L L+ ++  + + +W W   +  +      L  R+       L + F  E++K   D N  D  W  E+        D  L+ W    P DW    GG  ++Y  G+   GQL  +  + +    P  A S      ++ G Q  FA+  +G +YA G G+ G+LG+G +D    L P +   + H  +                    V+SWG+G+DGKLGHGN   C  PK+I +L G     +A G +HSA +T  G+++T+G G  GRLGHG+ + +  P+ I  L GY V  VACG    +          ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + +  +++ PKK+  L+   ++ IA G  H +A S  G V+ WG N  GQLG+++    + P ++  L GK I  ++ G  H+ AW++       NN+S    R     P   P E+  LL          RL LL+  +  I     +FP+  SD              S   +R  L   +      + + +TM      +++GP + + R+               G  S+F Q+  ++  +  + L LP+R WKV+ VGE+ DD GG + ++I+EMC EL    L LL PTPNG  D G N+D ++LN    S + +  F FLGVL G+A+RT  P+++NLA  VWK +  ++ T  DL ++D  Y   L  +      +    +L +P   F   S  G +VPL   ++  R+++EN+ EYV+ ALN R  E   Q+ AVR G+S + P P+L L     +E++VCG    PL +LK +A Y+ +D S  +I W WE++EEF N  + LFL FV GR+RLP    D   R    QIL +   P++ LP + TCFF L++P Y+ K +++ +L YAI  C++ID D Y
Sbjct: 2632 AKLEVSLQLEQLKAPDSALGSLLEELPQALLRQYEYEDVSVRAGLHLMHSDFFKVLVALACDLELDKMVGLVDNHKWSWFRKYCHASRVAKSLIHRSP------LPANFCQEVRKKLSDTN-ADGCWEHESHDIFKKEHDEQLLIWLNRRPEDWTLSWGGSGTIYGWGHNHRGQLGGIEGAKVKIPTPCEALSTLRPVQLVGGEQTLFAVTADGKVYATGYGAGGRLGIGGTDSV--LVPTLLESIQHVFIKKIAVNSGGKHCLALSADNDVYSWGEGDDGKLGHGNRLACETPKLIEALQGYEIIDIACGGAHSAAITSTGQLYTWGKGRYGRLGHGDSEDQLKPKLIEELVGYKVIDVACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPKKIESLAGLGVIKVECGSQFSVALTRSGSVYTWGKGDYHRLG-HGTGEHVRRPKKVAALQGKKIISIATGSLHCVACSDEGEVFTWGDNDEGQLGDDTTNAIQRPRLVSALQGKKITNVACGSAHTLAWST-------NNASSACAR----LPPVAPLEY-DLLQDIPTWVLHRRLVLLYYFAELICPCITMFPITGSD--------------SLHELRSILIYTIKEATFRKVVQATMVRD---KHHGPVIELNRIQVKRSRSRGGLAGVDGMKSVFGQMVTKLPLLTSETLALPHRVWKVEFVGESVDDCGGGYSESIAEMCDELQNGSLPLLIPTPNGRDDAGPNRDCFILNPTAKSCLHLNMFRFLGVLMGIAIRTGSPLSLNLAEPVWKQLAGMELTPADLTEIDRDYVPGLLCIREMGMEEPLFQNLEMP---FSTPSSCGTDVPL--STKYKRITFENRLEYVRLALNFRLHEFDEQIKAVRDGMSKVVPVPLLSLFSGYELETMVCGSPDIPLNLLKSVATYKGIDSSAPLIQWFWEVMEEFTNQERSLFLRFVWGRTRLPRTIADFRGRDFVIQILDKYNPPDHFLPESYTCFFLLKMPRYSCKHVLQQKLKYAIHFCKSIDKDEY 3622          

HSP 2 Score: 165.622 bits (418), Expect = 2.323e-39
Identity = 108/331 (32.63%), Postives = 170/331 (51.36%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKD-------NHFSNVHFPSPI-------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWTMR 584
            I  GS   +++   GK+ +C +         H +NV +P PI       I            +ALT D KV+SWG G  G+LGHG++ + + PK I +L    I  I+ G  H+AAIT  G LYTWG G  GRLGH D I    P L+K+L  H +  V+CG++   TL ++ +G  V+S+G G+ G+LG+G +     P  I   + + + ++    +FSLAL+ +G++W WGK  +       +     P  I S+    + +V+ G  H + + +   VY+WG N +G+ G G T +  K      L+   I+ +  G + S AWT++
Sbjct: 1848 IAGGSKSLFIVSHEGKLYACGEGTNGRLGLGHSNNVPYPRPIPFLSQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACGSRDAQTLALTDEGW-VFSWGDGDFGKLGRGGSEGCNIPHNIERLNALEVVQIECGAQFSLALTKTGEVWTWGKGDYFRLGHGSDQHVRKPTVIESLKDKKVIHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTIVNRKPALVHGLEDVHINRVACGSSHSIAWTLQ 2177          

HSP 3 Score: 164.466 bits (415), Expect = 5.284e-39
Identity = 109/336 (32.44%), Postives = 168/336 (50.00%), Query Frame = 0

Query: 2574 GGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            GGS S+       F +   G LYACGEG+ G+LGLG+S++     P+    LS  ++                    VFSWG+GEDGKLGHGN     +PK+I +L       +A G+SHSA +T  G+++T+G G+ GRLGHG+  T+  P+ I SL G+ +  VACG    +          F+W +                           V +I  G  FSL LT +G+V+  G   +  +  + S+ +++ P  I  L++  ++ +AVG  H LA++ +G V+ WG N  GQ GN +    ++P ++  L    I +++ G +HS AWT
Sbjct: 1850 GGSKSL-------FIVSHEGKLYACGEGTNGRLGLGHSNNVPYPRPIP--FLSQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACGSRDAQTLALTDEGWVFSWGDGDFGKLGRGGSEGCNIPHNIERLNALEVVQIECGAQFSLALTKTGEVWTWGKGDYFRLG-HGSDQHVRKPTVIESLKDKKVIHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTIVNRKPALVHGLEDVHINRVACGSSHSIAWT 2175          

HSP 4 Score: 149.058 bits (375), Expect = 2.406e-34
Identity = 98/280 (35.00%), Postives = 147/280 (52.50%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-----TSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAW-TMRKGKGCFHWP 593
            LAL+ D+ VYSWG G  G+LGHG++     PK I +L    I+ I+ G  H+AAIT  G LYTWG+G  GRLGH D      P L++ L  + V  V+CG+    TL ++ D   VWS+G G+ G+LG+G +     PK I    G+ + KV   ++FS+AL+ SG ++ WGK  ++     T   V  PK + ++    I +++TG  H +   +   V++WG N  G+ G   T+  ++ P+    L    I  +  G   + AW T      C   P
Sbjct: 2886 LALSADNDVYSWGEGDDGKLGHGNRLACETPKLIEALQGYEIIDIACGGAHSAAITSTGQLYTWGKGRYGRLGHGDSEDQLKPKLIEELVGYKVIDVACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKVPKKIESLAGLGVIKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGTGEHVRRPKKVAALQGKKIISIATGSLHCVACSDEGEVFTWGDNDEGQLGDDTTN-AIQRPRLVSALQGKKITNVACGSAHTLAWSTNNASSACARLP 3163          

HSP 5 Score: 98.9821 bits (245), Expect = 3.925e-19
Identity = 82/295 (27.80%), Postives = 137/295 (46.44%), Query Frame = 0

Query:  299 NGKVSCWKDNHF--------SNVHFPSPIIDASLSQ-----DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
            +G +  W  NH         + V  P+P    S  +       E  + A+T D KVY+ G G+ GRLG G       P  + S+  +    + +++G  H  A++ D  +Y+WGEG  G+LGH + +    P L++ L  + +  ++CG  H+  ++  G Q++++G G  G LG GD+     PKLI    G  +  V   +  +  L ++ D  +W WG   +            VPK I S++ +    V  G    + +  + SVY+WG   Y + G G T   V+ PK++
Sbjct: 2785 SGTIYGWGHNHRGQLGGIEGAKVKIPTPCEALSTLRPVQLVGGEQTLFAVTADGKVYATGYGAGGRLGIGGTDSVLVPTLLESIQHVFIKKIAVNSGGKHCLALSADNDVYSWGEGDDGKLGHGNRLACETPKLIEALQGYEIIDIACGGAHSAAITSTG-QLYTWGKGRYGRLGHGDSEDQLKPKLIEELVGYKVIDVACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPKKIESLAGLGVIKVECGSQFSVALTRSGSVYTWGKGDYHRLGHG-TGEHVRRPKKV 3077          

HSP 6 Score: 93.2041 bits (230), Expect = 1.903e-17
Identity = 63/215 (29.30%), Postives = 101/215 (46.98%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGS-SSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSD--------DYFSLTPVVTL-------LLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKI 2740
            +Y  G G+ G+L    + + + PK   S  G     V CG +   T AL   G +++ G+G +GKLG G S+        +  +   VV +       L       V++WG G+  +LGHG+ +  R+P +I SL       VAVG  H   +TD G+++ +G+ D G+ G+G     + P  +  L    +  VACG  H+    AW    T++
Sbjct: 1970 LYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACGSRDAQTLALTDEGWVFSWGDGDFGKLGRGGSEGCNIPHNIERLNALEVVQIECGAQFSLALTKTGEVWTWGKGDYFRLGHGSDQHVRKPTVIESLKDKKVIHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTIVNRKPALVHGLEDVHINRVACGSSHS---IAWTLQDTQV 2181          

HSP 7 Score: 91.2781 bits (225), Expect = 8.249e-17
Identity = 75/259 (28.96%), Postives = 125/259 (48.26%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFP---KEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVG----SVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            L++  E + L+      +Y WG+   G+LG  +      P   + +S+L P+  +  G     A+T DG +Y  G G+ GRLG        VPTL++++ +V     +V+ G +H L +S D   V+S+G G++G+LG G+     TPKLI    G  +  +      S A++ +G L+ WGK  +       +     PK I  +     +     +G    + I ++ +V+SWG   YGK G G  S   K PK+++
Sbjct: 2771 LNRRPEDWTLSWGGSGTIYGWGHNHRGQLGGIEGAKVKIPTPCEALSTLRPVQLV-GGEQTLFAVTADGKVYATGYGAGGRLGIGGTDSVLVPTLLESIQHVFIKKIAVNSGGKHCLALSADN-DVYSWGEGDDGKLGHGNRLACETPKLIEALQGYEIIDIACGGAHSAAITSTGQLYTWGKGRYGRLGHGDSEDQLKPKLIEELVGYKVIDVACGSGDAQTLCITDDDNVWSWGDGDYGKLGRG-GSDGCKVPKKIE 3026          

HSP 8 Score: 85.5001 bits (210), Expect = 4.687e-15
Identity = 82/300 (27.33%), Postives = 137/300 (45.67%), Query Frame = 0

Query:  314 HFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKE---ISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGR--LGHKDGIVH--FVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSL--KVKNPKRLDIYDIDGICAGPTQSYA-------WTMRKG 586
              P P   A+++QD+     + T   +V+ WG     +LG         P +   +S L PI  I+ G      ++ +G LY  GEG+ GR  LGH + + +   +P L + +    +V  G +H + ++ DG +V+S+G G +G+LG G+   L  PK+I       ++ +   +  S A++ SG+L+ WG   +       N +   PK I S+    I  V+ G      + + +   V+SWG   +GK G G +       N +RL+  ++  I  G   S A       WT  KG
Sbjct: 1786 QIPGPSTSANVAQDSTGEPSS-TVGCRVFVWGLNDKDQLGGMKGSKVKLPVQSDFLSQLWPIH-IAGGSKSLFIVSHEGKLYACGEGTNGRLGLGHSNNVPYPRPIPFLSQYVIKKVAVHSGGKHAMALTLDG-KVFSWGEGEDGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACGSRDAQTLALTDEGWVFSWGDGDFGKLGRGGSEGCNIPHNIERLNALEVVQIECGAQFSLALTKTGEVWTWGKG 2082          

HSP 9 Score: 75.485 bits (184), Expect = 4.376e-12
Identity = 60/217 (27.65%), Postives = 97/217 (44.70%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAG 2845
            VF WG  +  +LG     + + P     LS  +   +A G+    +++ +GK++  G G  GRLG G+      P  I  L  Y +  VA    H+               G   ++ LT  G VF  G  +  G   + + +N+  PK I  L  + + DIA G  H  A++ SG ++ WG    G+LG+       +P +I+ L G  I+Q++ G
Sbjct: 1812 VFVWGLNDKDQLGGMKGSKVKLPVQSDFLSQLWPIHIAGGSKSLFIVSHEGKLYACGEGTNGRLGLGHSNNVPYPRPIPFLSQYVIKKVAV---HS---------------GGKHAMALTLDGKVFSWGEGE-DGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACG 2009          

HSP 10 Score: 72.7886 bits (177), Expect = 2.766e-11
Identity = 39/107 (36.45%), Postives = 59/107 (55.14%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTL 433
            F +ALT    VY+WG G + RLGHG  +    PK++++L    I+ I+ G  H  A + +G ++TWG+   G+LG         P LV  L    + +V+CG+ HTL
Sbjct: 3042 FSVALTRSGSVYTWGKGDYHRLGHGTGEHVRRPKKVAALQGKKIISIATGSLHCVACSDEGEVFTWGDNDEGQLGDDTTNAIQRPRLVSALQGKKITNVACGSAHTL 3148          

HSP 11 Score: 67.3958 bits (163), Expect = 1.421e-9
Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 0

Query:  386 TLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSV--SCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTPWNT-------SSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG--LTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            T+   ++ WG     +LG   G    +P     L  +  +  + G++   IVS +G ++++ G G NG LG G +  +  P+ I F    ++KKV   +  + ++AL++ G ++ WG+            +   PK I ++ S  I +++ G  H   I  +  +Y+WGL  YG+ G G  +T LK K  K L  + I  +  G   +    +      F W
Sbjct: 1807 TVGCRVFVWGLNDKDQLGGMKGSKVKLPVQSDFLSQLWPIHIAGGSKSLFIVSHEG-KLYACGEGTNGRLGLGHSNNVPYPRPIPFLSQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNITQLKPKLIKSLVGHRIVQVACGSRDAQTLALTDEGWVFSW 2027          

HSP 12 Score: 65.855 bits (159), Expect = 3.917e-9
Identity = 54/178 (30.34%), Postives = 81/178 (45.51%), Query Frame = 0

Query: 2683 KIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTS--GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAV--GGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            ++F +G  D+ +LG       K P Q   L   +  ++A G                     + SLF+ S  G ++ CG      + +  SN N+  P+ IP L   V   +AV  GG H +AL+  G V+ WG    G+LG+ +     +P +IE L  K I  I+ G +HSAA TS
Sbjct: 1811 RVFVWGLNDKDQLGGMKGSKVKLPVQSDFLSQLWPIHIAGG---------------------SKSLFIVSHEGKLYACGEGTNGRLGLGHSN-NVPYPRPIPFLSQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLNLDKPKMIEALRSKKIRDIACGSSHSAAITS 1966          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: EFX81111.1 (hypothetical protein DAPPUDRAFT_50378 [Daphnia pulex])

HSP 1 Score: 434.491 bits (1116), Expect = 9.532e-121
Identity = 310/1027 (30.19%), Postives = 507/1027 (49.37%), Query Frame = 0

Query: 2417 FIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTV-LCSLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFD------EIKKD-VNLKD-----IVWTPEADSHLMKWYIVYPSDWQPG----GKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLT-PVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGL----------EHTKINFAWRES--------------------------VTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL--LPTTSAXECLTRLKLLHEISNYIVNVWRLFPV----IDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGP-----QVTVRRLFPIGSS-------SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENG----LPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
             ++     +++QYEYE+  +  G QL++S FLQ L  LA  L  + + +C+   SRW W   +  +    + L +R       L +S   D      EI+ D  N+ +     +V++ E D  L++W   +P DW       G   +Y  G+   GQL  +  + +  P   ++      +    G Q  FA+  +G +YA G   YG  G        S+T P +   L H  V                    V+SWG+GEDGKLGHGN   C RP+II SL G     VA G +HSA +T  G+++T+G G  GRLGHG+   +  P+ + +L GY V  VACG           E     ++W +                           + K+  G  FS+ L++ G V+  G   +  +  +  + +++ PKK+  L+   ++ I+ G  H +  +  G V+ WG N  GQLG+ +    ++P ++  L  K I ++S G  H+ AW+     T    S+P      +  P S+P ++  L  +P  +    L  L    EI   I+ +  L  V    +D +     +  +  +  ++D +R  L          + + +TM      R +GP     ++ +RR    G+S       ++F+Q+A ++  +    L LP+R+WKVK VGE+ DD GG + ++I+EMC+EL    L LL  TPNG  + G+N+D ++LN   ++  +++ F FLGVL G+A+RT  P+++NLA   WK ++ +  +  DL ++D  Y  +L ++    + D +  +L   D  F   S +G+EV L P + + R++ +N+D Y++ AL+ R  E  PQV AVR+GL  + P P+L L     +E++VCG    PL +LK +  Y+ ++P   ++ W WE++EE+++  + LFL FV GR+RLP    D   R  +L+V    N     LP + TCFF L++P Y+ K ++R +L YAI  C++ID D Y
Sbjct: 3475 LLRNLPEMLLQQYEYEEAAVRGGQQLLHSPFLQVLIALACDLRFDRLPICTNDGSRWSWFRRYCAAARAATGLMQRI-----RLPESFTADVKRKISEIQNDGANVGNDHEDPVVFSHEHDQQLLQWMNSHPEDWTQSWTAVGGGQIYGWGHNHRGQLGGVEGAKVKVPTVCEALSALRPIQLAGGEQTLFAITSDGRVYATG---YGAGGRLGIGGAESVTVPSLLESLQHVFVKKVAVNSGGKHCLALSSEGEVYSWGEGEDGKLGHGNKLPCDRPRIIESLRGKDVVDVACGGAHSAAITALGELYTWGKGRYGRLGHGDSDDQLKPKLVEALLGYRVMDVACGSGDAQTCAITDEEGGTVWSWGDGDYGKLGRGGSDGCKIPTKIESLSGLGIIKVECGSQFSVALSNNGSVYTWGKGDYHRLG-HGVDEHVRRPKKVMTLQGKKVISISTGALHCVVCTDQGEVYTWGDNDEGQLGDGTTTGIQKPRLVAALQNKKINRVSCGSAHTVAWS-----TCKGCSTPVAGSSRL--PTSIPLQYDLLKDIPIPALRNRLLLLHHFAEIVCPILPMLPLGTVNCRDMDGNVL---EETEGVLCGNHDRLRGILMASAKEAAFRKVVQATMIRD---RQHGPVVELNRIALRRSRATGTSGNAGPNSTVFAQMANKLSLLTYDVLMLPHRTWKVKFVGESVDDCGGGYSESIAEMCEELQNGSLPLLILTPNGREEAGTNRDCFILNPSASTAQNLKMFQFLGVLMGIAIRTGSPLSLNLAEPTWKALVGLPLSLIDLNEIDRHYWPALCHIRDCKDADGDV-TLSQLDLPFSTSSATGQEVALSPHTHR-RITRDNRDLYLQLALHFRLHEFDPQVRAVRQGLGQVVPLPLLSLFTGAELEAMVCGSPEIPLALLKSVTTYKGIEPHCALVRWFWEVMEEYSHVERSLFLRFVWGRTRLPRTLADFRGRDFVLQVLDKYNPADYFLPESYTCFFLLKMPRYSCKAVLREKLSYAIHFCKSIDNDEY 4477          

HSP 2 Score: 164.851 bits (416), Expect = 4.135e-39
Identity = 118/377 (31.30%), Postives = 184/377 (48.81%), Query Frame = 0

Query: 2542 GKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGS---SSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW------------RESVTKICL--------------GRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPT 2859
            G   VY+ G     QL     S I    A  +  S    SV  G +  F +  +G  YACGEGS G+LGLG+S +  S  P     LS ++V                    VFSWG+G+DGKLGHG+      P+ I +L+G     VA G++HSA +T  G+++T+G G+ GRLGHG+  T+ +P+++ SL G  V  V+CG        L    + ++W             E  +  CL              G  FS+ LT +G V+  G   +  +  + S+ +++ P  +  L  + ++ ++VG  H LA++ +G V+ WG N  GQ GN S    + P +++ + G  I +++ G +HS AWT+P  P+
Sbjct: 2696 GAKRVYVWGLNDKEQLGGPKGSKIKVPQAVDWTVSLQPISVAGGSKSLFLVTQSGKAYACGEGSNGRLGLGHSRNVNS--PHQITALSQHVVRKVAVHSGGRHALALTADGRVFSWGEGDDGKLGHGDVTSLDTPRCIEALNGFRVRDVAAGSNHSAAVTSSGELYTWGLGEYGRLGHGDNLTQLTPKRVESLVGQRVVQVSCGSRDAQTLALVEGGLVYSWGDGDFGKLGRGGSEGCSSPCLVDKLSGLGVCHVECGAQFSVALTCNGHVYTWGKGDYFRLG-HGSDQHVRWPTLVEGLRGKKVIGVSVGALHCLAVTDAGQVFAWGDNDHGQQGNGSITVNRRPTLVQGIDGIKITRVACGSSHSVAWTAPEPPS 3069          

HSP 3 Score: 156.377 bits (394), Expect = 1.757e-36
Identity = 97/273 (35.53%), Postives = 148/273 (54.21%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCG---AQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRKG 586
            LAL+ + +VYSWG G  G+LGHG+K   + P+ I SL    ++ ++ G  H+AAIT  G LYTWG+G  GRLGH D      P LV+ L  + V  V+CG   AQ   I  ++G  VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+ALS +G ++ WGK  +       +     PK + ++    + ++STG  H +V  +   VY+WG N  G+ G G T+  ++ P+    L    I+ +  G   + AW+  KG
Sbjct: 3714 LALSSEGEVYSWGEGEDGKLGHGNKLPCDRPRIIESLRGKDVVDVACGGAHSAAITALGELYTWGKGRYGRLGHGDSDDQLKPKLVEALLGYRVMDVACGSGDAQTCAITDEEGGTVWSWGDGDYGKLGRGGSDGCKIPTKIESLSGLGIIKVECGSQFSVALSNNGSVYTWGKGDYHRLGHGVDEHVRRPKKVMTLQGKKVISISTGALHCVVCTDQGEVYTWGDNDEGQLGDG-TTTGIQKPRLVAALQNKKINRVSCGSAHTVAWSTCKG 3985          

HSP 4 Score: 151.754 bits (382), Expect = 4.465e-35
Identity = 113/377 (29.97%), Postives = 186/377 (49.34%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKDN-------HFSNVHFPSPIIDASLSQ---------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW----NTSSTVPKWINSISSIS-----NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGI-----CAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYN--FGLSDFNAKLN 623
            +  GS   +++  +GK  +C + +       H  NV+ P  I   +LSQ               LALT D +V+SWG G  G+LGHGD    + P+ I +L    +  ++AG +H+AA+T  G LYTWG G  GRLGH D +    P  V++L    V  VSCG++   TL + + G+ V+S+G G+ G+LG+G +    +P L++   G+ +  V    +FS+AL+ +G ++ WGK  +    + S    +W   +  +       VS G  H + + +   V++WG N +G+ G G  ++   N +   +  IDGI       G + S AWT  +       P    P+   F  + D L +   GL++ +A++ 
Sbjct: 2736 VAGGSKSLFLVTQSGKAYACGEGSNGRLGLGHSRNVNSPHQI--TALSQHVVRKVAVHSGGRHALALTADGRVFSWGEGDDGKLGHGDVTSLDTPRCIEALNGFRVRDVAAGSNHSAAVTSSGELYTWGLGEYGRLGHGDNLTQLTPKRVESLVGQRVVQVSCGSRDAQTLALVEGGL-VYSWGDGDFGKLGRGGSEGCSSPCLVDKLSGLGVCHVECGAQFSVALTCNGHVYTWGKGDYFRLGHGSDQHVRWPTLVEGLRGKKVIGVSVGALHCLAVTDAGQVFAWGDNDHGQQGNGSITV---NRRPTLVQGIDGIKITRVACGSSHSVAWTAPEP------PSVAPPEPVPFPTIKDPLGSTAAGLTESSAEMG 3100          

HSP 5 Score: 102.064 bits (253), Expect = 4.793e-20
Identity = 80/273 (29.30%), Postives = 130/273 (47.62%), Query Frame = 0

Query:  321 DASLSQDNESFILALTE---DHKVYSWGNGSHGRLGHGDKKDYNFPKEIS---SLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGR--LGHKDGI--VHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGG--FHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC 572
            D++ ++  +S I  ++      +VY WG     +LG         P+ +    SL PI  ++ G      +T  G  Y  GEGS GR  LGH   +   H +  L +++    +V  G +H L ++ DG +V+S+G G++G+LG GD   L TP+ I   +G  ++ V A +  S A++ SG+L+ WG   +       N +   PK + S+    +  VS G      + ++E   VYSWG   +GK G G  S    +P  +D     G+C
Sbjct: 2677 DSAFNKSQQSVIQPISSVSGAKRVYVWGLNDKEQLGGPKGSKIKVPQAVDWTVSLQPI-SVAGGSKSLFLVTQSGKAYACGEGSNGRLGLGHSRNVNSPHQITALSQHVVRKVAVHSGGRHALALTADG-RVFSWGEGDDGKLGHGDVTSLDTPRCIEALNGFRVRDVAAGSNHSAAVTSSGELYTWGLGEYGRLGHGDNLTQLTPKRVESLVGQRVVQVSCGSRDAQTLALVEGGLVYSWGDGDFGKLGRG-GSEGCSSPCLVDKLSGLGVC 2946          

HSP 6 Score: 95.1301 bits (235), Expect = 5.780e-18
Identity = 78/297 (26.26%), Postives = 137/297 (46.13%), Query Frame = 0

Query:  299 NGKVSCWKDNH------FSNVHFPSPIIDASLSQ-------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVS----GDLWIWGKTPWNT-------SSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
             G++  W  NH              P +  +LS          E  + A+T D +VY+ G G+ GRLG G  +    P  + SL  +    + +++G  H  A++ +G +Y+WGEG  G+LGH + +    P ++++L   +V  V+CG  H+  ++  G +++++G G  G LG GD+     PKL+    G  +  V   +  +   +++    G +W WG   +            +P  I S+S +    V  G    + +  N SVY+WG   Y + G G+    V+ PK++
Sbjct: 3613 GGQIYGWGHNHRGQLGGVEGAKVKVPTVCEALSALRPIQLAGGEQTLFAITSDGRVYATGYGAGGRLGIGGAESVTVPSLLESLQHVFVKKVAVNSGGKHCLALSSEGEVYSWGEGEDGKLGHGNKLPCDRPRIIESLRGKDVVDVACGGAHSAAITALG-ELYTWGKGRYGRLGHGDSDDQLKPKLVEALLGYRVMDVACGSGDAQTCAITDEEGGTVWSWGDGDYGKLGRGGSDGCKIPTKIESLSGLGIIKVECGSQFSVALSNNGSVYTWGKGDYHRLGHGVDE-HVRRPKKV 3907          

HSP 7 Score: 85.8853 bits (211), Expect = 3.723e-15
Identity = 75/289 (25.95%), Postives = 127/289 (43.94%), Query Frame = 0

Query:  263 DIYTWG--------------CDLHSPKIWDDVN--DVEKIETGSSCTYVIHTNGKVSCWKDNHF-------SNVHFPSPIIDASL----------SQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSSTVPKWI 512
            ++Y+WG              CD   P+I + +   DV  +  G + +  I   G++  W    +       S+      +++A L          S D ++  +   E   V+SWG+G +G+LG G       P +I SL    I+ +  G   + A++ +G +YTWG+G   RLGH  G+   V  P  V  L    V S+S GA H ++ +  G +V+++G  + G+LG G T  +  P+L+       + +V   +  ++A S          TP   SS +P  I
Sbjct: 3721 EVYSWGEGEDGKLGHGNKLPCD--RPRIIESLRGKDVVDVACGGAHSAAITALGELYTWGKGRYGRLGHGDSDDQLKPKLVEALLGYRVMDVACGSGDAQTCAITDEEGGTVWSWGDGDYGKLGRGGSDGCKIPTKIESLSGLGIIKVECGSQFSVALSNNGSVYTWGKGDYHRLGH--GVDEHVRRPKKVMTLQGKKVISISTGALHCVVCTDQG-EVYTWGDNDEGQLGDGTTTGIQKPRLVAALQNKKINRVSCGSAHTVAWSTCKGC----STPVAGSSRLPTSI 4000          

HSP 8 Score: 67.3958 bits (163), Expect = 1.350e-9
Identity = 56/218 (25.69%), Postives = 101/218 (46.33%), Query Frame = 0

Query:  391 LYTWGEGSLGRLGHKDGIVHFVPTLVK---NLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTPWN-------TSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG--LTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            +Y WG     +LG   G    VP  V    +L  + SV+ G++   +V++ G + ++ G G+NG LG G +  + +P  I      +++KV   +  R +LAL+  G ++ WG+           TS   P+ I +++   + +V+ G  H   +  +  +Y+WGL  YG+ G G  LT L  K  + L    +  +  G   +    + +G   + W
Sbjct: 2700 VYVWGLNDKEQLGGPKGSKIKVPQAVDWTVSLQPI-SVAGGSKSLFLVTQSG-KAYACGEGSNGRLGLGHSRNVNSPHQITALSQHVVRKVAVHSGGRHALALTADGRVFSWGEGDDGKLGHGDVTSLDTPRCIEALNGFRVRDVAAGSNHSAAVTSSGELYTWGLGEYGRLGHGDNLTQLTPKRVESLVGQRVVQVSCGSRDAQTLALVEGGLVYSW 2915          

HSP 9 Score: 62.3882 bits (150), Expect = 3.723e-8
Identity = 64/225 (28.44%), Postives = 102/225 (45.33%), Query Frame = 0

Query:  389 GYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVS-CGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWG-----------KTPWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDI---YDI-DGIC-AGPTQSYAWTMRKGKGCFHW 592
            G +Y WG    G+LG  +G    VPT+ + L  +  +   G + TL       +V++ G G  G LG G    +  P L+     V +KKV   +  +  LALS  G+++ WG           K P +     P+ I S+    + +V+ GG H   I     +Y+WG   YG+ G G +  ++K PK ++    Y + D  C +G  Q+ A T  +G   + W
Sbjct: 3614 GQIYGWGHNHRGQLGGVEGAKVKVPTVCEALSALRPIQLAGGEQTLFAITSDGRVYATGYGAGGRLGIGGAESVTVPSLLESLQHVFVKKVAVNSGGKHCLALSSEGEVYSWGEGEDGKLGHGNKLPCDR----PRIIESLRGKDVVDVACGGAHSAAITALGELYTWGKGRYGRLGHGDSDDQLK-PKLVEALLGYRVMDVACGSGDAQTCAITDEEGGTVWSW 3833          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: EAL38855.3 (AGAP011823-PA, partial [Anopheles gambiae str. PEST])

HSP 1 Score: 431.409 bits (1108), Expect = 9.363e-120
Identity = 298/913 (32.64%), Postives = 454/913 (49.73%), Query Frame = 0

Query: 2519 WTPEADSHLMKWYIVYPSDW--QPGGKSSVYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPV--------IDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVA-TSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYM-LTRNNETRPTGGGD 3356
            +TP+ DS L++W+   P DW    GG S+++  G+   GQL  L  S I    P  A S      +  G Q  +A+ P+G LYA G G+ G+LG+G +D     TP +   L H ++                    VFSWG+GEDGKLGHGN +   RPK+I SLSG     +A G++HSA +T +G + T+G G  GRLGHG+ + +  P+ + +L GY    +ACG    +          ++W +                           VTK+  G  FS+ LT SG V+  G   +  +   +++ +++ PKK+  L+   ++ IA G  H +A S +G V+ WG N  GQLG+ +    + P +++ L GK I+++  G  H+ A ++      +  S   +P      P S P E+  L+   +      RL LLH  S  +     + P+        +     +  K   F   +    VR+     V  L  +Q   S M + N L               G  S+F Q+ +++  +  + L +P+R WKVK VGE+ DD GG F ++I+EMC EL    + LL  TPNG  + G+N+D +LL+    S + M  F FLGVL G+A+RT  P    LA  VW+ +        DL +VD  Y   L Y+     +P       LP   F   S  G EVPL   ++  +++ EN++EYVK ALN R  E   QV AVR G+S + P P+L L  +  ++++VCG    PL +LK +A Y+ ++ ++ ++ W WE++EEF N  + LFL FV GR+RLP    D   R  +L+V      P++ LP + TCFF L++P Y+ K +++ +L YAI  C++ID D Y  +   + T  TG  D
Sbjct: 4062 FTPQYDSQLLQWFNRRPEDWAFSWGGASTIFGWGHNHRGQLGGLDGSRIKNPTPCEALSLLRPIQIAGGEQTLYAVTPDGKLYATGYGAGGRLGVGGTDSV--TTPTLVESLQHVMIKKVAVNSGGKHCLALSSDGEVFSWGEGEDGKLGHGNRDSYDRPKLIESLSGIGVVDIACGSAHSACITIQGHVLTWGKGRYGRLGHGDSEDQLQPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKIESLAGLGVTKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGNTD-HVRRPKKVAALQGKKIISIATGSLHCVACSDAGEVFTWGDNDEGQLGDGTVSAIQRPRLVQSLQGKHIVKVICGSAHTLALST------YQLSEAVRP------PPSPPLEY-DLVRDIAPEVLHARLVLLHHFSELLCPCLAMLPIAGPLSLASLKDVLVYSIKETGFRKVIQTTMVRDKPHGPVIELNRIQVKRSRMRSGNGLAGVD-----------GMKSVFGQMVQKLPLLTQEALSMPHRVWKVKFVGESVDDCGGGFSESIAEMCDELQNGSVPLLIQTPNGRGEAGANRDCFLLDPTLTSVLHMNMFRFLGVLMGIAVRTGSP----LAEPVWRQLCGETLRPADLTEVDRDYITGLLYIRDVESDPKVFASIELP---FSTPSAKGHEVPL--STKYTKITPENRNEYVKLALNYRIHEFDEQVKAVRDGMSKVIPVPLLSLFSAAELQAMVCGSPDIPLCLLKTVATYKGVESTSPLVQWFWEVMEEFTNQERSLFLRFVWGRTRLPRTIADFRGRDFVLQVLDKYNPPDHFLPESYTCFFLLKMPRYSCKAVLQEKLKYAIYFCKSIDTDEYARVAMGDPTEATGSED 4938          

HSP 2 Score: 165.236 bits (417), Expect = 3.386e-39
Identity = 111/349 (31.81%), Postives = 176/349 (50.43%), Query Frame = 0

Query: 2574 GGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSP 2866
            GGS S+       F +  +G LYACGEG+ G+LGLG++++    TP    +LS  +V                    VFSWG+GEDGKLGHGN     +PK+I +L       +A G+SHSA +T  G+++T+G G+ GRLGHG+  T+  P+ +++L+ + V  VACG        L    + F+W +                           V +I  G  FSL LT +G+V+  G   +  +  + ++ +++ P  I  L  + ++ +AVG  H LA++ SG V+ WG N  GQ G+ +    K+P ++  L G  + +++ G +HS AW+ P   T  +   P
Sbjct: 3068 GGSKSL-------FIVSQDGKLYACGEGTNGRLGLGHNNNV--PTPKQVPILSQYVVKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLTLEKPKLIETLRTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNCTQLKPKLVTALQDHRVVQVACGSRDAQTLCLTEDGLVFSWGDGDFGKLGRGGSEGCSIPHQVDRLNGVGVMQIECGAQFSLALTKAGEVWTWGKGDYYRLG-HGTDQHVRKPTPIQGLRGKKVIHVAVGALHCLAVTDSGQVYAWGDNDHGQQGSGNTIVNKKPSLVLGLDGIFVNRVACGSSHSVAWSLPQNQTEKDKKEP 3406          

HSP 3 Score: 159.458 bits (402), Expect = 2.039e-37
Identity = 113/335 (33.73%), Postives = 172/335 (51.34%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKD-------NHFSNVHFPSPIIDASLSQ---------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-----NTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGI-----CAGPTQSYAWTM 583
            I  GS   +++  +GK+ +C +         H +NV  P+P     LSQ               +ALT D KV+SWG G  G+LGHG++     PK I +L    I  I+ G  H+AAIT  G LYTWG G  GRLGH D      P LV  L  H V  V+CG++   TL +++DG+ V+S+G G+ G+LG+G +     P  ++  +GV + ++    +FSLAL+ +G++W WGK  +      T   V  P  I  +    + +V+ G  H + + ++  VY+WG N +G+ G G T   + N K   +  +DGI       G + S AW++
Sbjct: 3066 IAGGSKSLFIVSQDGKLYACGEGTNGRLGLGHNNNV--PTPKQVPILSQYVVKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHGNRLTLEKPKLIETLRTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNCTQLKPKLVTALQDHRVVQVACGSRDAQTLCLTEDGL-VFSWGDGDFGKLGRGGSEGCSIPHQVDRLNGVGVMQIECGAQFSLALTKAGEVWTWGKGDYYRLGHGTDQHVRKPTPIQGLRGKKVIHVAVGALHCLAVTDSGQVYAWGDNDHGQQGSGNT---IVNKKPSLVLGLDGIFVNRVACGSSHSVAWSL 3394          

HSP 4 Score: 143.28 bits (360), Expect = 1.331e-32
Identity = 87/244 (35.66%), Postives = 136/244 (55.74%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI--MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSST-VPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK 561
            LAL+ D +V+SWG G  G+LGHG++  Y+ PK I SL  I  + I+ G  H+A IT+ G++ TWG+G  GRLGH D      P LV+ L  +    ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+AL+ SG ++ WGK  +      NT     PK + ++    I +++TG  H +   +   V++WG N  G+ G G  S  ++ P+
Sbjct: 4189 LALSSDGEVFSWGEGEDGKLGHGNRDSYDRPKLIESLSGIGVVDIACGSAHSACITIQGHVLTWGKGRYGRLGHGDSEDQLQPKLVEALLGYRAIDIACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKVPMKIESLAGLGVTKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGNTDHVRRPKKVAALQGKKIISIATGSLHCVACSDAGEVFTWGDNDEGQLGDGTVS-AIQRPR 4430          

HSP 5 Score: 135.576 bits (340), Expect = 2.863e-30
Identity = 99/335 (29.55%), Postives = 158/335 (47.16%), Query Frame = 0

Query:  280 VNDVEKIETGSSCTYVIHTNGKV-------SCWKDNHFSNVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVS--CGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGK-CGLGLTSLKVKNPKRLD---IYDIDGICAGPTQSYAWTMRKGKG 588
            ++ V +I    +C Y++  +G V       S  +DN+ + V      I    +      +LAL     VYSWG+G  GRLGHGD      P  I++L    +  +  G  ++AAIT  G LYTWG G+ GRLGH +    +VPTLV  L  H V  V+  CG  H+L V++ G+  +++G G+ G+LG         P  ++      + KV + ++FS+AL+  G ++ WGK          +  S VPK + ++    I +++ G  H + +  +  +Y WG N + + C   +T   +     L       + G+ AG  QS  W      G
Sbjct:  562 LDQVRQIVCAENCVYILTDSGTVLYLSHAISMTEDNYPAQVKGLECTIVQLAAHCEGRHVLALASSGDVYSWGSGDGGRLGHGDTSAKELPTRIAALADRQVTGVFCGSSYSAAITASGELYTWGRGTYGRLGHGNSEDKYVPTLVTALKAHRVVHVALGCGDAHSLCVTEAGL-AFAWGDGDFGKLGNESCVGTSVPVQVDLPQASCVVKVFSGSQFSVALTREGTVYTWGKGHGGRLGHGNSEHSPVPKMVQALEGKKIVDLAVGLAHCLALTASGELYGWGRNDFQQICPECVTRDPIITAPILTTPPTLRVAGMAAGVAQSLFWCHSSSHG 895          

HSP 6 Score: 115.546 bits (288), Expect = 3.841e-24
Identity = 71/226 (31.42%), Postives = 116/226 (51.33%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKI--PKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            V+SWG G+ G+LGHG+T     P  I++L+    T V  G+S+SA +T  G+++T+G G  GRLGHGN + K  P  +++L+ + V +VA G                   G   SL +T +G  F  G+  F  +  N S +    P ++  P+   +V +  G    +AL++ G V+ WG    G+LG+ +      P +++ L GK I+ ++ G  H  A T+
Sbjct:  630 VYSWGSGDGGRLGHGDTSAKELPTRIAALADRQVTGVFCGSSYSAAITASGELYTWGRGTYGRLGHGNSEDKYVPTLVTALKAHRVVHVALGC------------------GDAHSLCVTEAGLAFAWGDGDFGKLG-NESCVGTSVPVQVDLPQASCVVKVFSGSQFSVALTREGTVYTWGKGHGGRLGHGNSEHSPVPKMVQALEGKKIVDLAVGLAHCLALTA 836          

HSP 7 Score: 102.064 bits (253), Expect = 4.378e-20
Identity = 66/202 (32.67%), Postives = 100/202 (49.50%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLA--DLGSSSIPPKYAD-SFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV------------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            VY  G+G  G+L   D  +  +P + A  +    + V CG   + A+  +G LY  G G+YG+LG GNS+D +  T +VT L +H +V V                  F+WGDG+ GKLG+ +      P  +     +    V  G+  S  LT +G ++T+G G  GRLGHGN +    P+ + +L G  +  +A GL H
Sbjct:  630 VYSWGSGDGGRLGHGDTSAKELPTRIAALADRQVTGVFCGSSYSAAITASGELYTWGRGTYGRLGHGNSEDKYVPT-LVTALKAHRVVHVALGCGDAHSLCVTEAGLAFAWGDGDFGKLGNESCVGTSVPVQVDLPQASCVVKVFSGSQFSVALTREGTVYTWGKGHGGRLGHGNSEHSPVPKMVQALEGKKIVDLAVGLAH 830          

HSP 8 Score: 96.2857 bits (238), Expect = 2.207e-18
Identity = 76/270 (28.15%), Postives = 121/270 (44.81%), Query Frame = 0

Query: 2581 CGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXVF----------------SWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGT--SHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPII 2830
            C  +   AL  +G +Y+ G G  G+LG G++     L   +  L    +  VF                +WG G  G+LGHGN+E    P ++++L  +    VA+G   +HS  +T+ G  F +G+GD G+LG+ +      P Q+       +   +C              V K+  G  FS+ LT     +       G   + ++ +   PK +  LE   +VD+AVG  H LAL+ SG ++GWG N   Q+  E     ++PII
Sbjct:  616 CEGRHVLALASSGDVYSWGSGDGGRLGHGDTSAK-ELPTRIAALADRQVTGVFCGSSYSAAITASGELYTWGRGTYGRLGHGNSEDKYVPTLVTALKAHRVVHVALGCGDAHSLCVTEAGLAFAWGDGDFGKLGNESCVGTSVPVQVD------LPQASC--------------VVKVFSGSQFSVALTREGTVYTWGKGHGGRLGHGNSEHSPVPKMVQALEGKKIVDLAVGLAHCLALTASGELYGWGRNDFQQICPEC--VTRDPII 862          

HSP 9 Score: 95.9005 bits (237), Expect = 2.722e-18
Identity = 82/289 (28.37%), Postives = 135/289 (46.71%), Query Frame = 0

Query:  305 WKDNHF--------SNVHFPSPIIDASLSQ-----DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIM----CISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSV--SCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWINSIS--SISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
            W  NH         S +  P+P    SL +       E  + A+T D K+Y+ G G+ GRLG G       P  + SL  +M     +++G  H  A++ DG +++WGEG  G+LGH +   +  P L+++L  +G V  +CG+ H+  ++  G  V ++G G  G LG GD+     PKL+    G     +   +  +  L ++ D  +W WG   +            VP  I S++   ++ V  G    + +  + SVY+WG   Y + G G T   V+ PK++
Sbjct: 4094 WGHNHRGQLGGLDGSRIKNPTPCEALSLLRPIQIAGGEQTLYAVTPDGKLYATGYGAGGRLGVGGTDSVTTPTLVESLQHVMIKKVAVNSGGKHCLALSSDGEVFSWGEGEDGKLGHGNRDSYDRPKLIESLSGIGVVDIACGSAHSACITIQG-HVLTWGKGRYGRLGHGDSEDQLQPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKIESLAGLGVTKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGNTD-HVRRPKKV 4380          

HSP 10 Score: 90.1225 bits (222), Expect = 1.871e-16
Identity = 65/238 (27.31%), Postives = 109/238 (45.80%), Query Frame = 0

Query:  274 PKIWDDVND--VEKIETGSSCTYVIHTNGKVSCWKDNHFSNV-------HFPSPIIDASLS--------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSV 490
            PK+ + ++   V  I  GS+ +  I   G V  W    +  +            +++A L            ++  L +T+D  V+SWG+G +G+LG G       P +I SL    +  +  G   + A+T  G +YTWG+G   RLGH +      P  V  L    + S++ G+ H +  S D  +V+++G  + G+LG G    +  P+L+    G  + KVI  +  +LALS 
Sbjct: 4219 PKLIESLSGIGVVDIACGSAHSACITIQGHVLTWGKGRYGRLGHGDSEDQLQPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKVPMKIESLAGLGVTKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGNTDHVRRPKKVAALQGKKIISIATGSLHCVACS-DAGEVFTWGDNDEGQLGDGTVSAIQRPRLVQSLQGKHIVKVICGSAHTLALST 4455          

HSP 11 Score: 83.9593 bits (206), Expect = 1.153e-14
Identity = 69/229 (30.13%), Postives = 111/229 (48.47%), Query Frame = 0

Query:  341 VYSWGNGSHGRLGHGDKKDYNFP---KEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVG----SVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            ++ WG+   G+LG  D      P   + +S L PI  I+ G     A+T DG LY  G G+ GRLG         PTLV++L +V     +V+ G +H L +S DG +V+S+G G +G+LG G+      PKLI    G+ +  +   +  S  +++ G +  WGK  +       +     PK + ++     +     +G    + I ++ +V+SWG   YGK G G
Sbjct: 4091 IFGWGHNHRGQLGGLDGSRIKNPTPCEALSLLRPIQ-IAGGEQTLYAVTPDGKLYATGYGAGGRLGVGGTDSVTTPTLVESLQHVMIKKVAVNSGGKHCLALSSDG-EVFSWGEGEDGKLGHGNRDSYDRPKLIESLSGIGVVDIACGSAHSACITIQGHVLTWGKGRYGRLGHGDSEDQLQPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRG 4317          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: EEB14993.1 (hect E3 ubiquitin ligase, putative [Pediculus humanus corporis])

HSP 1 Score: 431.024 bits (1107), Expect = 1.062e-119
Identity = 311/1011 (30.76%), Postives = 489/1011 (48.37%), Query Frame = 0

Query: 2417 FIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLPDS-RWKWLSSFTQSMTFISLLNKRNAKSDHELL--KSLFFDEIKKDVNLK-----DIVWTPEADSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIR-LSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRV----ERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
             +K     ++ QYEYE+  +  G  L++S F + L  LA  L L++   S  +  +W W   +  +    S L  RN+   +  L  K    + I  D  ++       ++  E D  L+ W    P DW    GG  ++Y  G+   GQL  +    +  P   ++      V  + G Q   A+  +G +YA G G+ G+LGLG +D    LTP +   + H  +                    V+SWG+G+DGKLGHGN   C  P++I +L G     +A G +HSA +T  G+I+T+G G  GRLGHG+ + +  P+ + +L G+ V  +ACG    +          ++W +                           V K+  G  FS+ LT SG V+  G   +  +  + S+ +++ PKKI  L+   ++ IA G  H +A S  G V+ WG N  GQLG+ +      P ++  L GK I +++ G  HS AW+     TN + S+          P   P E+  L+   S      RL LLH  S  +     +FP+                  S D +R+ L   V      + + +TM      + +GP + + R+             P G  S+F Q+  ++  +  + L LP+R WKVK VGE+ DD GG + ++I+EMC EL    L LL  TPNG  D G+N+D +LLN +  +   ++ F FLG+L G+A+RT  P+++NLA  VWK +  +  T  DL ++D  Y   L  +    +P  +    L    F   S SG +VPL   S + R ++ +N+ EYV+ ALN R  E   Q+ AVR+G++ + P P+L L     +E++VCG    PL +LK +A Y+ +D +  ++ W WEI+E+F N  + LFL F  GR+RLP    D   R  +L+V      P++ LP + TCFF L++P Y+ K +++ +L YAI  C++++ D Y
Sbjct: 3831 LLKGLPQALLRQYEYEEPLVKNGRHLMHSSFFKVLVALACDLGLDSFSHSSENHHKWCWFRRYCIASRVASSLINRNSLPQNFCLEVKKKIAEMIPDDETIQCHHEDHALFKQEQDRQLLLWLNRKPEDWTLTWGGSGTIYGWGHNHRGQLGGVEGPKVKIPTSCEALSALRPVQLVGGEQTLLAVTSDGKVYATGYGAGGRLGLGGTDTI--LTPTLLESIQHIFIKKVAVNSGGKHCLALSTDGKVYSWGEGDDGKLGHGNKNGCDHPQLIDALQGKEIVDIACGGAHSAAVTAGGEIYTWGKGRYGRLGHGDSEDQMKPKLVETLIGFKVIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVVKVECGSQFSVALTRSGSVYTWGKGDYHRLG-HGSDEHVRHPKKIAALQGKKIICIATGSLHCVACSDLGEVFTWGDNDEGQLGDGTMNAIHRPRLVVTLQGKNINRVACGSAHSLAWS-----TNKSTSA-------THLPVDAPMEY-DLVRDISLPTLRNRLVLLHHFSELVSPNVAMFPMEGEG--------------SLDQLRDILVYSVKEATFRKVIQTTMVRD---KQHGPVIELNRIQVKRSRSKGGLAGPDGMKSVFGQMVNKMSLLTQESLFLPHRVWKVKFVGESVDDCGGGYSESIAEMCDELQNGSLPLLIVTPNGRDDTGTNRDCFLLNPNAKTTHHLQMFKFLGILMGIAIRTGSPLSLNLAEPVWKQLAGMTLTPADLTEIDRDYVPGLLCI-RDMDPSEKVFQTLEMP-FSTPSASGVDVPL---STRYRYVTIDNRQEYVRLALNCRLHEFDKQIAAVREGMAKVIPVPLLSLFSGYELETMVCGSQDIPLNLLKSVATYKGIDATAPLVQWFWEIMEDFTNQERSLFLRFAWGRTRLPRTIADFRGRDFVLQVLDTYNPPDHFLPESYTCFFLLKMPRYSCKSVLQQKLKYAINFCKSLETDEY 4803          

HSP 2 Score: 162.54 bits (410), Expect = 2.357e-38
Identity = 113/370 (30.54%), Postives = 179/370 (48.38%), Query Frame = 0

Query: 2544 SSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSV--ICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAW-----------------------RESVTKICL---GRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            + V++ G     QL  L  S +  P Y++S      +    G +  F +  +G LYACGEG+ G+LGLG+S++    TP     L+  ++                    VFSWG+GEDGKLGH N     RPK+I +L       +A G+SHSA ++  G++FT+G G+ GRLGHG+  T+  P+ + +L G+ +  VACG        L    + F+W                       R +   +C+   G  FSL LT  G V+  G   +  +  + S+ +++ P  +  L  + +V +AVG  H L  +  G V+ WG N  GQ GN +    ++P ++  L G  + +++ G +HS AWT+
Sbjct: 2985 TKVFVWGLNDKDQLGGLKGSKVKVPVYSESLTALKPIHIAGGSKSLFIVSYDGKLYACGEGTNGRLGLGHSNNV--ATPCQLTSLNQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHDNRLSLDRPKMIEALKSKRIRDIACGSSHSAAISSTGELFTWGLGEYGRLGHGDNLTQLRPKLVKALLGHHIVQVACGSRDAQTLALSAEGLVFSWGDGDFGKLGRGGSEGCDIPHNIERLNGLGVCMIECGAQFSLALTKFGQVYTWGKGDYFRLG-HGSDQHVRKPTLVESLSEKKIVHVAVGALHCLCATDQGQVYAWGDNDHGQQGNGTILVNRKPSLVLNLEGVRVNRVACGSSHSIAWTT 3351          

HSP 3 Score: 151.754 bits (382), Expect = 4.157e-35
Identity = 97/279 (34.77%), Postives = 145/279 (51.97%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRKGKGCFHWP 593
            LAL+ D KVYSWG G  G+LGHG+K   + P+ I +L    I+ I+ G  H+AA+T  G +YTWG+G  GRLGH D      P LV+ L    V  ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+AL+ SG ++ WGK  +       +     PK I ++    I  ++TG  H +   +   V++WG N  G+ G G T   +  P+    L   +I+ +  G   S AW+  K     H P
Sbjct: 4068 LALSTDGKVYSWGEGDDGKLGHGNKNGCDHPQLIDALQGKEIVDIACGGAHSAAVTAGGEIYTWGKGRYGRLGHGDSEDQMKPKLVETLIGFKVIDIACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVVKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGSDEHVRHPKKIAALQGKKIICIATGSLHCVACSDLGEVFTWGDNDEGQLGDG-TMNAIHRPRLVVTLQGKNINRVACGSAHSLAWSTNKSTSATHLP 4344          

HSP 4 Score: 150.984 bits (380), Expect = 6.113e-35
Identity = 118/381 (30.97%), Postives = 184/381 (48.29%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKD-------NHFSNVHFPSP-------IIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFDEDEQQK 633
            I  GS   +++  +GK+ +C +         H +NV  P         +I            +ALT D KV+SWG G  G+LGH ++   + PK I +L    I  I+ G  H+AAI+  G L+TWG G  GRLGH D +    P LVK L  H++  V+CG++   TL +S +G+ V+S+G G+ G+LG+G +     P  I   +G+ +  +    +FSLAL+  G ++ WGK  +       +     P  + S+S   I +V+ G  H +   +   VY+WG N +G+ G G T L  + P     L+   ++ +  G + S AWT        H P   EP    F+ + D L    L     K +S  +E+E+ K
Sbjct: 3023 IAGGSKSLFIVSYDGKLYACGEGTNGRLGLGHSNNVATPCQLTSLNQYVIKKVAVHSGGKHAMALTLDGKVFSWGEGEDGKLGHDNRLSLDRPKMIEALKSKRIRDIACGSSHSAAISSTGELFTWGLGEYGRLGHGDNLTQLRPKLVKALLGHHIVQVACGSRDAQTLALSAEGL-VFSWGDGDFGKLGRGGSEGCDIPHNIERLNGLGVCMIECGAQFSLALTKFGQVYTWGKGDYFRLGHGSDQHVRKPTLVESLSEKKIVHVAVGALHCLCATDQGQVYAWGDNDHGQQGNG-TILVNRKPSLVLNLEGVRVNRVACGSSHSIAWTTSD----THAPNMTEP--VLFTALKDPLGALCL-----KTDSVVNEEEESK 3390          

HSP 5 Score: 141.739 bits (356), Expect = 4.471e-32
Identity = 101/271 (37.27%), Postives = 144/271 (53.14%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-------WNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL------DIYDIDGICAGPTQSYAWT 582
            LAL+ D  VYSWGNG  GRLGHGD    + P +I+ L    ++ IS G  ++AA+T  G +YTWG G+ GRLGH     H VPTLV  L +  +  ++CG+    TL V+  G+ V+S+G G  G+LG G +    TPKLI     + + +V   ++FSLAL+ SG ++ WGK                PK +  ++   I  V+ G  H++ + ++  VY WG N  G+ G   +   V  P  L      +I DID    GP QS AW+
Sbjct:  534 LALSCDGDVYSWGNGDGGRLGHGDNNSRDQPTKITELVGKNVIHISCGATYSAAVTSSGEVYTWGRGNYGRLGHGTSEDHTVPTLVVGLKDYFIVDIACGSGDAQTLAVTNTGL-VFSWGDGGYGKLGTGGSDGSKTPKLIEKLQDIEVARVYCGSQFSLALTKSGSVYTWGKGDNHRLGHGSENHVRFPKLVEGLNGKCIKKVAVGSGHVLALTDDGEVYGWGRNDNGQVG-ETSETNVSEPTLLTLLQGKNIVDID---CGPNQSIAWS 799          

HSP 6 Score: 140.969 bits (354), Expect = 7.089e-32
Identity = 85/260 (32.69%), Postives = 129/260 (49.62%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            V+SWG+G+ G+LGHG+     +P  I+ L G     ++ G ++SA +T  G+++T+G G+ GRLGHG  +    P  +  L+ YF+  +ACG        + +T + F+W +                           V ++  G  FSL LT SG V+  G      +   S N +++ PK +  L    +  +AVG  HVLAL+  G V+GWG N  GQ+G  S     EP ++  L GK I+ I  G N S AW+S
Sbjct:  542 VYSWGNGDGGRLGHGDNNSRDQPTKITELVGKNVIHISCGATYSAAVTSSGEVYTWGRGNYGRLGHGTSEDHTVPTLVVGLKDYFIVDIACGSGDAQTLAVTNTGLVFSWGDGGYGKLGTGGSDGSKTPKLIEKLQDIEVARVYCGSQFSLALTKSGSVYTWGKGDNHRLGHGSEN-HVRFPKLVEGLNGKCIKKVAVGSGHVLALTDDGEVYGWGRNDNGQVGETSETNVSEPTLLTLLQGKNIVDIDCGPNQSIAWSS 800          

HSP 7 Score: 95.5153 bits (236), Expect = 3.993e-18
Identity = 89/281 (31.67%), Postives = 125/281 (44.48%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLA--DLGSSSIPPKYADSFGGSS-SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTL-----------------LLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKS 2803
            VY  GNG  G+L   D  S   P K  +  G +   + CG   + A+  +G +Y  G G+YG+LG G S+D+   T VV L                 L   N   VFSWGDG  GKLG G ++  + PK+I  L       V  G+  S  LT  G ++T+G GD  RLGHG+    + P+ +  L G  +  VA G  H                     L LT  G+V+  G +  +G    +S  N+  P  +  L+  N+VDI  G +  +A S S
Sbjct:  542 VYSWGNGDGGRLGHGDNNSRDQPTKITELVGKNVIHISCGATYSAAVTSSGEVYTWGRGNYGRLGHGTSEDHTVPTLVVGLKDYFIVDIACGSGDAQTLAVTNTGLVFSWGDGGYGKLGTGGSDGSKTPKLIEKLQDIEVARVYCGSQFSLALTKSGSVYTWGKGDNHRLGHGSENHVRFPKLVEGLNGKCIKKVAVGSGHV--------------------LALTDDGEVYGWGRND-NGQVGETSETNVSEPTLLTLLQGKNIVDIDCGPNQSIAWSSS 801          

HSP 8 Score: 94.3597 bits (233), Expect = 9.383e-18
Identity = 63/210 (30.00%), Postives = 99/210 (47.14%), Query Frame = 0

Query: 2550 GNGQNGQLADLGS-SSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSDD----------------YFSLTPVVTLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVT 2738
            G G+ G+L    + + + PK   +  G     V CG +   T AL   G +++ G+G +GKLG G S+                         +L L+     V++WG G+  +LGHG+ +  R+P ++ SLS      VAVG  H    TD+G+++ +G+ D G+ G+G     + P  + +L G  V  VACG  H+    AW  S T
Sbjct: 3149 GLGEYGRLGHGDNLTQLRPKLVKALLGHHIVQVACGSRDAQTLALSAEGLVFSWGDGDFGKLGRGGSEGCDIPHNIERLNGLGVCMIECGAQFSLALT-KFGQVYTWGKGDYFRLGHGSDQHVRKPTLVESLSEKKIVHVAVGALHCLCATDQGQVYAWGDNDHGQQGNGTILVNRKPSLVLNLEGVRVNRVACGSSHS---IAWTTSDT 3354          

HSP 9 Score: 92.8189 bits (229), Expect = 2.438e-17
Identity = 75/255 (29.41%), Postives = 126/255 (49.41%), Query Frame = 0

Query:  328 NESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGH--KDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
             E  +LA+T D KVY+ G G+ GRLG G       P  + S+  I    + +++G  H  A++ DG +Y+WGEG  G+LGH  K+G  H  P L+  L    +  ++CG  H+  V+  G +++++G G  G LG GD+     PKL+    G  +  +   +  +  L ++ D  +W WG   +            +P  I S++ +    V  G    + +  + SVY+WG   Y + G G +   V++PK++
Sbjct: 4009 GEQTLLAVTSDGKVYATGYGAGGRLGLGGTDTILTPTLLESIQHIFIKKVAVNSGGKHCLALSTDGKVYSWGEGDDGKLGHGNKNGCDH--PQLIDALQGKEIVDIACGGAHSAAVTAGG-EIYTWGKGRYGRLGHGDSEDQMKPKLVETLIGFKVIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVVKVECGSQFSVALTRSGSVYTWGKGDYHRLGHG-SDEHVRHPKKI 4259          

HSP 10 Score: 86.2705 bits (212), Expect = 2.702e-15
Identity = 66/246 (26.83%), Postives = 119/246 (48.37%), Query Frame = 0

Query:  324 LSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFP---KEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            L++  E + L       +Y WG+   G+LG  +      P   + +S+L P+  +  G     A+T DG +Y  G G+ GRLG         PTL++++ ++     +V+ G +H L +S DG +V+S+G G++G+LG G+      P+LI+   G  +  +      S A++  G+++ WGK  +       +     PK + ++     +     +G    + I ++ +V+SWG   YGK G G
Sbjct: 3953 LNRKPEDWTLTWGGSGTIYGWGHNHRGQLGGVEGPKVKIPTSCEALSALRPVQLV-GGEQTLLAVTSDGKVYATGYGAGGRLGLGGTDTILTPTLLESIQHIFIKKVAVNSGGKHCLALSTDG-KVYSWGEGDDGKLGHGNKNGCDHPQLIDALQGKEIVDIACGGAHSAAVTAGGEIYTWGKGRYGRLGHGDSEDQMKPKLVETLIGFKVIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRG 4196          

HSP 11 Score: 79.337 bits (194), Expect = 2.753e-13
Identity = 61/206 (29.61%), Postives = 96/206 (46.60%), Query Frame = 0

Query:  263 DIYTWGC------------DLHSPKIWDDVNDVEKIE----TGSSCTYVIHTNGKVSCWKDNHFSNVHFPS------PIIDASLS-------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLH--NVGSVSCGAQHTL 433
            +IYTWG             D   PK+ + +   + I+    +G + T  I  +  V  W D  +  +          P+   SL+       +    F +ALT    VY+WG G + RLGHG  +    PK+I++L    I+CI+ G  H  A +  G ++TWG+   G+LG  DG ++ +  P LV  L   N+  V+CG+ H+L
Sbjct: 4127 EIYTWGKGRYGRLGHGDSEDQMKPKLVETLIGFKVIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKIPMKIESLAGLGVVKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGSDEHVRHPKKIAALQGKKIICIATGSLHCVACSDLGEVFTWGDNDEGQLG--DGTMNAIHRPRLVVTLQGKNINRVACGSAHSL 4330          

HSP 12 Score: 77.0258 bits (188), Expect = 1.375e-12
Identity = 61/238 (25.63%), Postives = 109/238 (45.80%), Query Frame = 0

Query: 2654 PKIISSLSGNFATFVAVGT--SHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI------NFAW-RESVTKICLGRTF-----SLFLTSGDVFF----CGNSKFSGMDINSSNM------------------NIQSPKKIPKLENM--VDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            P+ I    G   + +A      HS  L+  G ++++GNGD GRLGHG+  ++  P +I+ L G  V +++CG  ++         + W R +  ++  G +      +L +   D F     CG+     + + ++ +                    ++PK I KL+++    +  G    LAL+KSG V+ WG     +LG+ S    + P ++E L+GK I +++ G  H  A T
Sbjct:  510 PEQIEGFDGKEISKLAAHADGKHSLALSCDGDVYSWGNGDGGRLGHGDNNSRDQPTKITELVGKNVIHISCGATYSAAVTSSGEVYTWGRGNYGRLGHGTSEDHTVPTLVVGLKDYFIVDIACGSGDAQTLAVTNTGLVFSWGDGGYGKLGTGGSDGSKTPKLIEKLQDIEVARVYCGSQFSLALTKSGSVYTWGKGDNHRLGHGSENHVRFPKLVEGLNGKCIKKVAVGSGHVLALT 747          

HSP 13 Score: 68.1662 bits (165), Expect = 7.800e-10
Identity = 38/107 (35.51%), Postives = 54/107 (50.47%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTL 433
            F LALT+   VY+WG G + RLGHG +    FPK +  L    I  ++ G  H  A+T DG +Y WG    G++G         PTL+  L   N+  + CG   ++
Sbjct:  690 FSLALTKSGSVYTWGKGDNHRLGHGSENHVRFPKLVEGLNGKCIKKVAVGSGHVLALTDDGEVYGWGRNDNGQVGETSETNVSEPTLLTLLQGKNIVDIDCGPNQSI 796          

HSP 14 Score: 60.4622 bits (145), Expect = 1.392e-7
Identity = 55/189 (29.10%), Postives = 90/189 (47.62%), Query Frame = 0

Query:  389 GYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSC--GAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIWGKTP-------WNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLD 564
            G +Y WG    G+LG  +G    +PT  + L  +  V    G Q  L V+ DG +V++ G G  G LG G T  + TP L+     + +KKV   +  +  LALS  G ++ WG+                P+ I+++    I +++ GG H   +     +Y+WG   YG+ G G +  ++K PK ++
Sbjct: 3968 GTIYGWGHNHRGQLGGVEGPKVKIPTSCEALSALRPVQLVGGEQTLLAVTSDG-KVYATGYGAGGRLGLGGTDTILTPTLLESIQHIFIKKVAVNSGGKHCLALSTDGKVYSWGEGDDGKLGHGNKNGCDHPQLIDALQGKEIVDIACGGAHSAAVTAGGEIYTWGKGRYGRLGHGDSEDQMK-PKLVE 4154          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: AAF50913.3 (HECT and RLD domain containing protein 2 [Drosophila melanogaster])

HSP 1 Score: 416.001 bits (1068), Expect = 3.626e-115
Identity = 295/912 (32.35%), Postives = 441/912 (48.36%), Query Frame = 0

Query: 2524 DSHLMKWYIVYPSDWQP--GGKSSVYLVGNGQNGQLADLGSSSIP---PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI--------NFAWRES--------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRR------------LFPI-GSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE-TRPTGGGD 3356
            D+ L++W    P DW    GG S++Y  G+   GQL  L  S I    P  A S      +  G Q  FA+ P+G L+A G GS G+LG+G SD +    P +   L H  V                    V++WG+GEDGKLGHGN     RPK++  L+G     +A G++HSA +T  G + T+G G  GRLGHG+ + +  P+ + +L GY    +ACG    +          ++W +                           V K+  G  FS+ LT SG V+  G   F  +   S + +++ PKK+  L+   ++ IA G  H +A S SG V+ WG N  GQLG+ +    + P ++  L GK I++++ G  H+ A ++  +                 RP   P     L+   +      RL LLH  S  +     + P I  D             +S  A+++ L   +      + + +TM      + +GP + + R            L  I G  S+F Q+ +++  +  + L LP+R WKVK VGE+ DD GG + ++I+EMC EL    + LL  TPNG  + G+N+D +LL+   +S + M  F FLGVL G+A+RT  P++INLA  VW+ +        DL +VD  Y   L  +    N D++       +  F   S  G EVPL   +    +S  N+ EYV+ AL  R  E   QV AVR G+S + P P+L L  +  ++++VCG    PL +LK +A Y+  DPS+ ++ W WE++EEF N  + LFL FV GR+RLP    D   R    Q+L    P++ LP + TCFF L++P Y+ K ++  +L YAI  C++ID D Y      E T  TG  D
Sbjct: 4027 DAQLLQWLNRRPDDWALSWGGASTIYGWGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWA--IPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVD-HVRRPKKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDGTVTAIQRPRLVAALQGKHIVKVTCGSAHTLALSTSQLSERL-------------RPLPNPPLEYDLVRDLAPEALHARLILLHHFSELVCPCLAMLP-ISGD-------------LSLGALKDVLVYNIKEAAFRKVIQTTMVRD---KQHGPVIELNRIQVKRSRNRCNGLAGIDGMKSVFGQMVQKLPLLTQEALALPHRVWKVKFVGESVDDCGGGYSESIAEMCDELQNGSVPLLINTPNGRGEAGANRDCFLLDPTLSSVLQMNMFRFLGVLMGIAVRTGSPLSINLAEPVWRQLTGEVLRPTDLTEVDRDYVAGLLCIR---NMDDDPKLFTALELPFSTSSARGHEVPL--STRYTHISPRNRAEYVRLALGFRLHEFDEQVKAVRDGMSKVIPVPLLSLFSAAELQAMVCGSPDIPLGLLKSVATYKGFDPSSALVTWFWEVMEEFTNQERSLFLRFVWGRTRLPRTIADFRGRDFVLQVLEKNPPDHFLPESYTCFFLLKMPRYSCKAVLLEKLKYAIHFCKSIDTDEYARVAMGEPTEATGSED 4900          

HSP 2 Score: 156.762 bits (395), Expect = 1.245e-36
Identity = 105/347 (30.26%), Postives = 168/347 (48.41%), Query Frame = 0

Query: 2574 GGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDD------------YFSLTPVVTLLLSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSP 2866
            GGS S+       F +  +G +YACGEG+ G+LGLG + +            Y      V     H L       VFSWG+GEDGKLGHGN     +P+++ +L       VA G+SHSA ++ +G+++T+G G+ GRLGHG+  T+  P+ +++L G  V  VACG        L      F+W +                           V +I  G  FSL LT +G+V+  G   +  +  +  + +++ P+ I  L    ++ +AVG  H LA++ +G V+ WG N  GQ G+ + +  K+P ++  L    + +++ G +HS AW  P   ++     P
Sbjct: 3026 GGSKSL-------FIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLG-HGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVACGSSHSIAWGLPNASSDEEKRGP 3364          

HSP 3 Score: 151.754 bits (382), Expect = 3.931e-35
Identity = 103/331 (31.12%), Postives = 164/331 (49.55%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKDNHFS-------NVHFPSP-------IIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWTM 583
            I  GS   +++  +GKV +C +  +         NV  P         ++            LALT D KV+SWG G  G+LGHG++   + P+ + +L    I  ++ G  H+AAI+  G LYTWG G  GRLGH D      P LV  L    V  V+CG++   TL +++DG  V+S+G G+ G+LG+G +    TP  I    G+ + ++    +FSLAL+ +G++W WGK  +       +     P+ I  +    + +V+ G  H + + +   VY+WG N +G+ G G T +  K P     LD   ++ +  G + S AW +
Sbjct: 3024 IAGGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGA-VFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVN-KKPALVIGLDAVFVNRVACGSSHSIAWGL 3352          

HSP 4 Score: 139.043 bits (349), Expect = 2.928e-31
Identity = 84/244 (34.43%), Postives = 134/244 (54.92%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWNTSS-------TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK 561
            LALT + +VY+WG G  G+LGHG++  Y+ PK +  L    +  I+ G  H+AAIT  G++ TWG+G  GRLGH D      P LV+ L  +    ++CG+    TL ++ D   VWS+G G+ G+LG+G +     P  I    G+ + KV   ++FS+AL+ SG ++ WGK  ++            PK + ++    I +++TG  H +   ++  VY+WG N  G+ G G T   ++ P+
Sbjct: 4149 LALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDD-NVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVDHVRRPKKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDG-TVTAIQRPR 4390          

HSP 5 Score: 119.783 bits (299), Expect = 2.079e-25
Identity = 87/277 (31.41%), Postives = 136/277 (49.10%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW---------NTSSTVPKWINSIS--SISNVSTGGFHLIVILENRSVYSWGLNTYGK-CGLGLTS---LKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKG 588
            LA+  +H  YSWG G   RLGHGD      P +I++L    +  +  G  ++AAIT  G L TWG G+  RLGH +     +PTLV  L  H V  V+ G+   H+L ++ +G+ V+++G G+ G+LG G+      P L+     V  ++V A ++FS+ALS  G L+ WGK                +VP+ ++S+    I +V+    H + +  +  V+ WG N   + C   ++S   L+      L  +   GI     QS  WT    +G
Sbjct:  629 LAIDSNHNAYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGL-VFAWGDGDYGKLGNGNCNGSLQPILVESLPRV--QRVFAGSQFSVALSSEGQLYTWGKATCLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQICPASVSSEPLLRTPILVSLPTFPASGIACTSAQSLVWTQSSHQG 902          

HSP 6 Score: 100.908 bits (250), Expect = 9.859e-20
Identity = 66/211 (31.28%), Postives = 101/211 (47.87%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADL-GSSSIPPKYADSFGGSS--SVICGLQ--VTFALQPNGSLYACGEGSYGKLGLGNSD--------DYFSLTPVVTL-------LLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
            +  +Y  G G+ G+L     ++ + PK   +  G     V CG +   T AL  +G++++ G+G +GKLG G S+        +  S   VV +       L       V++WG G+  +LGHG  +  R+P+ I  L G     VAVG  H   +TD G+++ +G+ D G+ G GN    K P  +  L   FV  VACG  H+    AW
Sbjct: 3143 QGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGSEGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVACGSSHS---IAW 3350          

HSP 7 Score: 94.7449 bits (234), Expect = 7.309e-18
Identity = 68/217 (31.34%), Postives = 99/217 (45.62%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRES------------------------VTKICLGRTFSLFLTS-GDVFFCGNSKFSGMD-INSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCN 2812
             +SWG GED +LGHG+T     P  I++L  +    V  G S+SA +T  G + T+G G   RLGHGN   +  P  + +L  + V  VA G        L    + FAW +                         V ++  G  FS+ L+S G ++  G +   G   +  S      P+ +  L++  +VD+AV   H LALS SG V+GWG N
Sbjct:  637 AYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEGQLYTWGKATCLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRN 853          

HSP 8 Score: 94.7449 bits (234), Expect = 7.622e-18
Identity = 72/264 (27.27%), Postives = 118/264 (44.70%), Query Frame = 0

Query:  263 DIYTWG------------CDLHSPKIWDDVN--DVEKIETGSSCTYVIHTNGKVSCWKDNHFSNV-------HFPSPIIDASLS--------QDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVS 491
            ++Y WG                 PK+ + +N   V  I  GS+ +  I  +G V  W    +  +            +++A L            ++  L +T+D  V+SWG+G +G+LG G       P +I SL    ++ +  G   + A+T  G +YTWG+G   RLGH  G V  V  P  V  L    + S++ G+ H +  S  G +V+++G  + G+LG G    +  P+L+    G  + KV   +  +LALS S
Sbjct: 4156 EVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGH--GSVDHVRRPKKVAALQGKKIISIATGSLHCVACSDSG-EVYTWGDNDEGQLGDGTVTAIQRPRLVAALQGKHIVKVTCGSAHTLALSTS 4416          

HSP 9 Score: 92.0485 bits (227), Expect = 4.139e-17
Identity = 78/289 (26.99%), Postives = 136/289 (47.06%), Query Frame = 0

Query:  305 WKDNHF--------SNVHFPSPIIDASLSQ-----DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPI----MCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGD--LWIWGKTPWNT-------SSTVPKWINSISSIS--NVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRL 563
            W  NH         S +  P+P    SL +       E  + A+T D K+++ G GS GRLG G    +  P  + SL  +    + +++G  H  A+T +G +Y WGEG  G+LGH + + +  P LV++L+  +V  ++CG+ H+  ++  G  V ++G G  G LG GD+     PKL+    G     +   +  +  L ++ D  +W WG   +            +P  I S++ +    V  G    + + ++ +VY+WG   + + G G     V+ PK++
Sbjct: 4054 WGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWAIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASG-HVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHGSVD-HVRRPKKV 4340          

HSP 10 Score: 84.7297 bits (208), Expect = 7.645e-15
Identity = 53/177 (29.94%), Postives = 96/177 (54.24%), Query Frame = 0

Query:  330 SFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCI----SAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFG---CGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGK 499
            S+  A+T    + +WG G++ RLGHG+  D + P  + +L   M +     +G  H+ A+T +G ++ WG+G  G+LG+ +      P LV++L  V  V  G+Q ++ +S +G Q++++G   C  +  + +   GC   P+L++      +  V  S    LALS SG+++ WG+
Sbjct:  678 SYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEG-QLYTWGKATCLGHQLVERSVQGC-SVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGR 852          

HSP 11 Score: 82.0333 bits (201), Expect = 4.827e-14
Identity = 65/228 (28.51%), Postives = 112/228 (49.12%), Query Frame = 0

Query:  341 VYSWGNGSHGRLG--HGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV----GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNV----STGGFHLIVILENRSVYSWGLNTYGKCGLG 551
            +Y WG+   G+LG   G +     P E  SL   + ++ G     A+T DG L+  G GS GRLG        +PTL+ +L +V     +V+ G +H L ++ +G +V+++G G +G+LG G+      PKL+   +G+ +  +   +  S A++ SG +  WGK  +       +     PK + ++     +     +G    + I ++ +V+SWG   YGK G G
Sbjct: 4051 IYGWGHNHRGQLGGLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSWAIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEG-EVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRG 4277          

HSP 12 Score: 74.7146 bits (182), Expect = 7.413e-12
Identity = 69/228 (30.26%), Postives = 102/228 (44.74%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLA--DLGSSSIPPKYAD-SFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLV------------------XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHG----NFQTKKSPEQISSLRGYFVTYVACGLEH------TKINFAW-RESVTKIC 2741
             Y  G G++ +L   D  + ++P K A        SV CG   + A+   G+L   G G+Y +LG GNSDD  SL  +V  L  H +V                   VF+WGDG+ GKLG+GN     +P ++ SL      F   G+  S  L+ +G+++T+G      LGH     + Q    P  +SSL+   +  VA  + H      +   F W R    +IC
Sbjct:  637 AYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDR-SLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVF--AGSQFSVALSSEGQLYTWGKAT--CLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQIC 859          

HSP 13 Score: 71.2478 bits (173), Expect = 9.864e-11
Identity = 55/217 (25.35%), Postives = 94/217 (43.32%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAG 2845
            V  WG  +  +LG     + + P    ++S      +A G+    +++  GK++  G G  GRLG G       P Q+  LR Y V  VA    H+               G   +L LT  G VF  G  +  G   + +   +  P+ +  L  + + D+A G  H  A+S  G ++ WG    G+LG+       +P ++  L+G+ ++Q++ G
Sbjct: 2988 VMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAV---HS---------------GGKHALALTLDGKVFSWGEGE-DGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACG 3185          

HSP 14 Score: 70.4774 bits (171), Expect = 1.512e-10
Identity = 63/236 (26.69%), Postives = 105/236 (44.49%), Query Frame = 0

Query:  375 ISAGHHHAAAIT---LDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSV--SCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVI--ASNRFSLALSVSGDLWIW-----GKTPWNTSSTV--PKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG--LTSLKVKNPKRLDIYDIDGICAGPTQSYAWTMRKGKGCFHW 592
            +S  H  AAA     L   +  WG     +LG   G    VPT  + +  +  +  + G++   IVS+DG +V++ G G NG LG G T  +  P  +      ++KKV   +  + +LAL++ G ++ W     GK      +T+  P+ + ++ +  I +V+ G  H   I     +Y+WGL  YG+ G G   T LK K    L    +  +  G   +    + +    F W
Sbjct: 2969 VSTSHEEAAAAPEQDLPCTVMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLFIVSQDG-KVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSW 3203          

HSP 15 Score: 62.7734 bits (151), Expect = 3.165e-8
Identity = 45/140 (32.14%), Postives = 74/140 (52.86%), Query Frame = 0

Query:  319 IIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHF------VPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNN 450
            ++D +L    ++  LALT +  V++WG+G +G+LG+G+      P  + SLP +  + AG   + A++ +G LYTWG+ +   LGH+  +V        VP LV +L +  +  V+    H L +S  G     FG G N
Sbjct:  722 VVDVALG-SGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLPRVQRVFAGSQFSVALSSEGQLYTWGKATC--LGHQ--LVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGE---VFGWGRN 853          

HSP 16 Score: 62.003 bits (149), Expect = 5.319e-8
Identity = 50/192 (26.04%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 2667 FVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVG-GD-HVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            F+A+ ++H+A        +++G G+  RLGHG+   +  P +I++L  + V  V CG  ++                   +     G++   G   ++ +   +S+ +   P  +  L +  +VD+A+G GD H LAL+  G V+ WG    G+LGN +     +PI++E L    + ++ AG   S A +S
Sbjct:  628 FLAIDSNHNA--------YSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYS-------------------AAITCGGNLLTWGRGTYARLGHGNSD-DRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESL--PRVQRVFAGSQFSVALSS 789          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: gb|KFM72733.1| (E3 ubiquitin-protein ligase HERC2, partial [Stegodyphus mimosarum])

HSP 1 Score: 323.939 bits (829), Expect = 1.682e-94
Identity = 211/637 (33.12%), Postives = 329/637 (51.65%), Query Frame = 0

Query: 2737 VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL----LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRL------------FPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDY-FMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVER----PENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNET 3349
            V K+  G  FS+ LT SG V+  G   +  +  + ++ +++ P+K+  L N  +  IAVG  H +    +G V+ WG N  GQLG+ S    + P ++  L+GK I +++ G  H+ AW++             K  H+   P+++P E+  L    +P      CL    L H    +  ++    P+ D               + ND +R  L          + L +TM      R +GP + + R+               G+ S+F Q+   +  I  + L LP+R WKVK VGE+ DD GG + ++++EMC EL    L LL  TPNG  D G+N+D +LLN    S + +  F FLG+L G+A+RT  P+++N+A  VWK +  +  T  DL +VD  Y   L  V+  +    E +SL   D  F   S +G EVPL   S+   ++ EN+ EY++ ALN R  E   QV AVR+G+S + P P+L L     +E++VCG    P+ +LK +A Y+ ++P   ++ W WE++EEFNN  + LFL FV GR+RLP    D   R  +L+V      P++ LP + TCFF L++P Y+ K +++ +L YAI  C++ID D Y     N++
Sbjct:    3 VIKVECGSQFSVALTRSGSVYTWGKGDYHRLG-HGTDDHVRRPRKVSALHNKKVTSIAVGSLHCVVCIDTGEVYTWGDNDEGQLGDGSTNAIQRPRLVPALTGKKINKVACGSAHTVAWST------------CKTSHSGRLPSTVPLEYDHLRDFSIPVLRNRYCL----LYHFSELFCASI----PMFD---------------LGNDNLRGLLVSSGKEAAFRKVLQATMIRD---RQHGPVIELNRMQVKKSRSKGGLAGNDGAKSVFGQMVSNMSLISQESLLLPHRVWKVKFVGESVDDCGGGYSESVAEMCDELQNGSLPLLILTPNGRDDAGTNKDCFLLNPLAKSSLHLDMFRFLGMLMGIAMRTGSPLSLNMAEPVWKQLTGMSLTSSDLTEVDKDYVPGL--VSIRY---MEAESLRKFDLPFSTHSATGAEVPL--SSKYSCITPENRVEYIRLALNYRLHEFDEQVSAVREGMSKVVPVPLLSLFTGSELETMVCGSPDIPIQLLKAVATYKGIEPDAPLVQWFWEVMEEFNNPERSLFLRFVWGRTRLPRTIADFRGRDFVLQVMEKYNPPDHFLPESYTCFFLLKMPRYSCKIVLKEKLKYAIHFCKSIDTDDYARVALNQS 593          

HSP 2 Score: 85.8853 bits (211), Expect = 1.323e-15
Identity = 39/102 (38.24%), Postives = 60/102 (58.82%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAW 2733
            V++WG G+  +LGHG  +  RRP+ +S+L     T +AVG+ H  V  D G+++T+G+ D G+LG G+    + P  + +L G  +  VACG  HT    AW
Sbjct:   22 VYTWGKGDYHRLGHGTDDHVRRPRKVSALHNKKVTSIAVGSLHCVVCIDTGEVYTWGDNDEGQLGDGSTNAIQRPRLVPALTGKKINKVACGSAHT---VAW 120          

HSP 3 Score: 67.781 bits (164), Expect = 6.151e-10
Identity = 39/109 (35.78%), Postives = 57/109 (52.29%), Query Frame = 0

Query:  331 FILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFV--PTLVKNL--HNVGSVSCGAQHTL 433
            F +ALT    VY+WG G + RLGHG       P+++S+L    +  I+ G  H       G +YTWG+   G+LG  DG  + +  P LV  L    +  V+CG+ HT+
Sbjct:   12 FSVALTRSGSVYTWGKGDYHRLGHGTDDHVRRPRKVSALHNKKVTSIAVGSLHCVVCIDTGEVYTWGDNDEGQLG--DGSTNAIQRPRLVPALTGKKINKVACGSAHTV 118          

HSP 4 Score: 65.855 bits (159), Expect = 1.996e-9
Identity = 37/119 (31.09%), Postives = 62/119 (52.10%), Query Frame = 0

Query: 2575 GSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX----------------VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAV 2677
            G   V CG Q + AL  +GS+Y  G+G Y +LG G +DD+      V+ L +  +                  V++WGD ++G+LG G+T   +RP+++ +L+G     VA G++H+  
Sbjct:    2 GVIKVECGSQFSVALTRSGSVYTWGKGDYHRLGHG-TDDHVRRPRKVSALHNKKVTSIAVGSLHCVVCIDTGEVYTWGDNDEGQLGDGSTNAIQRPRLVPALTGKKINKVACGSAHTVA 119          

HSP 5 Score: 65.4698 bits (158), Expect = 3.272e-9
Identity = 38/121 (31.40%), Postives = 62/121 (51.24%), Query Frame = 0

Query:  372 IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSV 490
            ++ +  G   + A+T  G +YTWG+G   RLGH        P  V  LHN  V S++ G+ H  +V  D  +V+++G  + G+LG G T  +  P+L+    G  + KV   +  ++A S 
Sbjct:    3 VIKVECGSQFSVALTRSGSVYTWGKGDYHRLGHGTDDHVRRPRKVSALHNKKVTSIAVGSLHC-VVCIDTGEVYTWGDNDEGQLGDGSTNAIQRPRLVPALTGKKINKVACGSAHTVAWST 122          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: gb|KYB24996.1| (hect domain and RLD 2-like protein [Tribolium castaneum])

HSP 1 Score: 157.532 bits (397), Expect = 6.008e-37
Identity = 95/268 (35.45%), Postives = 145/268 (54.10%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGA--QHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSI--SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWT 582
            LALT+D++VYSWGNG  GRLGHGD      P  I +L    I+ +  G  ++AAI+ +G LYTWG G+ GRLGH        PT++  L   ++  V+CG+   HTL ++  G +V+S+G G+ G+LG+G +     P+LI     V + +V    +FS+ALS  G ++ WGK        P   +   P+ +  +    +  VS G  H++ + +   VY WG N   K     + L V+ P+ ++      + G+C GP QS+AWT
Sbjct:  535 LALTKDYEVYSWGNGDGGRLGHGDTSTKEEPTLIQALKGKDIIDVECGGTYSAAISANGALYTWGRGNYGRLGHGTAEDCLTPTMISALSDEHIIKVACGSFYAHTLCITSQG-KVYSWGDGDYGKLGRGGSDGSKLPRLIEKLQNVKIVQVYCGAQFSVALSEDGHVYTWGKGEGWKLGHPTEENVRFPEMVEVLRDRKVVGVSLGVSHVLALTDQGEVYGWGKNE-NKQICDSSELFVQQPRLIEALKGQKVVGVCCGPAQSFAWT 800          

HSP 2 Score: 125.946 bits (315), Expect = 2.328e-27
Identity = 85/289 (29.41%), Postives = 141/289 (48.79%), Query Frame = 0

Query: 2587 FALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV----------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGT--SHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDI-NSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
             AL  +  +Y+ G G  G+LG G++      T ++  L   +++ V                ++WG G  G+LGHG  E C  P +IS+LS      VA G+  +H+  +T +GK++++G+GD G+LG G     K P  I  L+   +  V CG +                    FS+ L+  G V+  G  K  G  + + +  N++ P+ +  L +  +V +++G  HVLAL+  G V+GWG N   Q+ + S  F ++P +IE L G+ ++ +  G   S AWT
Sbjct:  535 LALTKDYEVYSWGNGDGGRLGHGDTSTKEEPT-LIQALKGKDIIDVECGGTYSAAISANGALYTWGRGNYGRLGHGTAEDCLTPTMISALSDEHIIKVACGSFYAHTLCITSQGKVYSWGDGDYGKLGRGGSDGSKLPRLIEKLQNVKIVQVYCGAQ--------------------FSVALSEDGHVYTWG--KGEGWKLGHPTEENVRFPEMVEVLRDRKVVGVSLGVSHVLALTDQGEVYGWGKNENKQICDSSELFVQQPRLIEALKGQKVVGVCCGPAQSFAWT 800          

HSP 3 Score: 103.219 bits (256), Expect = 1.883e-20
Identity = 79/285 (27.72%), Postives = 126/285 (44.21%), Query Frame = 0

Query: 2529 KWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTL------------LLSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAV 2792
            K Y+    D++      VY  GNG  G+L    +S+   P    +  G     V CG   + A+  NG+LY  G G+YG+LG G ++D  + T +  L              +H L       V+SWGDG+ GKLG G ++  + P++I  L       V  G   S  L++ G ++T+G G+  +LGH   +  + PE +  LR   V  V+ G+ H                     L LT  G+V+  G ++   +  +SS + +Q P+ I  L+    + V
Sbjct:  532 KHYLALTKDYE------VYSWGNGDGGRLGHGDTSTKEEPTLIQALKGKDIIDVECGGTYSAAISANGALYTWGRGNYGRLGHGTAEDCLTPTMISALSDEHIIKVACGSFYAHTLCITSQGKVYSWGDGDYGKLGRGGSDGSKLPRLIEKLQNVKIVQVYCGAQFSVALSEDGHVYTWGKGEGWKLGHPTEENVRFPEMVEVLRDRKVVGVSLGVSHV--------------------LALTDQGEVYGWGKNENKQI-CDSSELFVQQPRLIEALKGQKVVGV 789          

HSP 4 Score: 81.2629 bits (199), Expect = 8.434e-14
Identity = 58/212 (27.36%), Postives = 92/212 (43.40%), Query Frame = 0

Query: 2545 SVYLVGNGQNGQLA-DLGSSSIPPKYADSFGGSS--SVICG--LQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXVF----------------SWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRE 2735
            ++Y  G G  G+L        + P    +        V CG     T  +   G +Y+ G+G YGKLG G SD    L  ++  L +  +V V+                +WG GE  KLGH   E  R P+++  L       V++G SH   LTD+G+++ +G  +  ++   +    + P  I +L+G  V  V CG   +   FAW +
Sbjct:  594 ALYTWGRGNYGRLGHGTAEDCLTPTMISALSDEHIIKVACGSFYAHTLCITSQGKVYSWGDGDYGKLGRGGSDGS-KLPRLIEKLQNVKIVQVYCGAQFSVALSEDGHVYTWGKGEGWKLGHPTEENVRFPEMVEVLRDRKVVGVSLGVSHVLALTDQGEVYGWGKNENKQICDSSELFVQQPRLIEALKGQKVVGVCCGPAQS---FAWTD 801          

HSP 5 Score: 75.485 bits (184), Expect = 4.130e-12
Identity = 59/217 (27.19%), Postives = 100/217 (46.08%), Query Frame = 0

Query: 2674 HSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------------------------------LEHTKINFAWRESVTKICLGRTFS--LFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWT 2853
            H   LT   +++++GNGD GRLGHG+  TK+ P  I +L+G  +  V CG                                L  T I+    E + K+  G  ++  L +TS G V+  G+  +  +    S+ + + P+ I KL+N  +V +  G    +ALS+ G V+ WG     +LG+ +    + P ++E L  + ++ +S G +H  A T
Sbjct:  533 HYLALTKDYEVYSWGNGDGGRLGHGDTSTKEEPTLIQALKGKDIIDVECGGTYSAAISANGALYTWGRGNYGRLGHGTAEDCLTPTMISALSDEHIIKVACGSFYAHTLCITSQGKVYSWGDGDYGKLGRGGSDGS-KLPRLIEKLQNVKIVQVYCGAQFSVALSEDGHVYTWGKGEGWKLGHPTEENVRFPEMVEVLRDRKVVGVSLGVSHVLALT 748          

BLAST of EMLSAG00000002245 vs. Select Arthropod Genomes
Match: gb|KFM56578.1| (E3 ubiquitin-protein ligase HERC2, partial [Stegodyphus mimosarum])

HSP 1 Score: 155.221 bits (391), Expect = 7.244e-37
Identity = 117/331 (35.35%), Postives = 169/331 (51.06%), Query Frame = 0

Query:  286 IETGSSCTYVIHTNGKV-SCWKD-------NHFSNVHFPSPIIDASLSQ---------DNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP--IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQ--HTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSI--SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP--KRLDIYDIDGICAGPTQSYAWT 582
            I  GS   ++I  +GKV +C +         H +NV FP  I   SLSQ               LALT D KV+SWG G  G+LGHG++  Y  P+ I +L    I  I+ G  H+AAI+  G LYTWG G  GRLGH D +    P +V       V  VSCG++   TL ++ D M V+S+G G+ G+LG+G +     P+ I   +G+ +  V    +FSLAL+ SG +W WGK  +        +    P+ I S+    I +V+ G  H + + ++  VY+WG N +G+ G G T++  K    + LD   I  +  G + S AWT
Sbjct:  295 ISGGSKSLFIISQDGKVYACGEGTNGRLGLGHSNNVSFPRQIY--SLSQFVIRKIAVHSGGRHALALTIDGKVFSWGEGDDGKLGHGNRMTYEKPRLILALKSKRIRDIACGSAHSAAISSSGELYTWGLGDYGRLGHGDLVTQTKPKMVMAFAGKRVKQVSCGSRDAQTLALTDDDM-VYSWGDGDFGKLGRGGSEGCRVPENIESLNGLGVIHVECGAQFSLALTNSGQVWTWGKGDYFRLGHGEESHVRRPQLIESLRDKKIIHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPALVQGLDDVKITRVACGSSHSIAWT 622          

HSP 2 Score: 154.066 bits (388), Expect = 1.695e-36
Identity = 123/419 (29.36%), Postives = 201/419 (47.97%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC--GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDY------FSLTPVVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG--------LEHTKINFAWRE--------------------------SVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPM--PTNFNNSSPTKPRHNIG------RPNSLPDEFVSLLPTTSAXECL 2896
            ++ VY+ G     QL  L  S I  P ++++      +    G +  F +  +G +YACGEG+ G+LGLG+S++       +SL+  V   ++      H L       VFSWG+G+DGKLGHGN     +P++I +L       +A G++HSA ++  G+++T+G GD GRLGHG+  T+  P+ + +  G  V  V+CG        L    + ++W +                           V  +  G  FSL LT SG V+  G   +  +  +    +++ P+ I  L +  ++ +AVG  H LA++ SG V+ WG N  GQ GN +    ++P +++ L    I +++ G +HS AWT+  +  P   +      PR  +G        +SLPDE   L  T+S   C 
Sbjct:  256 ETKVYVWGLNDKDQLGGLRGSKIKLPAFSETLSALKPITISGGSKSLFIISQDGKVYACGEGTNGRLGLGHSNNVSFPRQIYSLSQFVIRKIAVHSGGRHALALTIDGKVFSWGEGDDGKLGHGNRMTYEKPRLILALKSKRIRDIACGSAHSAAISSSGELYTWGLGDYGRLGHGDLVTQTKPKMVMAFAGKRVKQVSCGSRDAQTLALTDDDMVYSWGDGDFGKLGRGGSEGCRVPENIESLNGLGVIHVECGAQFSLALTNSGQVWTWGKGDYFRLG-HGEESHVRRPQLIESLRDKKIIHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPALVQGLDDVKITRVACGSSHSIAWTTVEVQTPRLLDPVLFPAPRDPLGALSLGKNDSSLPDE---LDATSSNKTCF 670          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|1126188166|ref|XP_019626885.1| (PREDICTED: LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HERC1 [Branchiostoma belcheri])

HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0
Identity = 493/1306 (37.75%), Postives = 706/1306 (54.06%), Query Frame = 0

Query: 2167 LATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLVLSVGNI-IYIWDMEDSEENCNQ------IPQLSPIIYLCWSP----NSEIEILLAARQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQR-----------------------KESIHLYGSQI-VSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP---------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLK---DIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRH-NIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLF--------PVIDSDFYWPPKIKDFYI-AMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYS---RSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE 3348
             A G  DG +R+   + Y      D D   T+E    S   LTW P+G  L     ++ + +W       NC+       IP  SP+  + W P      E +++LA   +  S+ + TL    ++  ++I + +      Q + R                       +E + L G Q  + +L FH      LASG S+   G+VN+WS+   +V +TL+G G    L W   + LA  F  +++V+LM    +    +  +A+   A   + +     AP  +A + +    + EQY YEK  +  G QL++S FLQN + +A  L L  VLC++P                  W WL+SF+ ++     L+KR A     ++  +  + I KD  ++   ++ W    D  +M W    P DW  GG+   Y+ G G++GQLA+ G S + P    SF  +  +ICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     +A G  HSAV++  GK+FTFGNGD GRLG GN   KK PE++++L G  V  V CGL HT                                 +++      V K+C G  FS+ LT  G VF  G  +  G    S + N   P+++P LE   + D+ VG +H LAL+ +G VW WG N  GQLG       +EP ++  L GK + QISAG+NHS  WT+PP P      +P  P    +G+P+S+P +F SL    S      RL+LLH  S+ I + WRL          VI S       +    +  +    +R  L+P V TLP+++ +  TM      +NYGPQ+TVRRL   G     IF QIA QV  + P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   V++LL PTPN  +++G N+D+++LN    ++  +  F FLGVL GVA+RT+KP+ ++LAP+VWK +  +  T  D+EDVD  +    RS+  +  S         ++P D F G S  GR VP+ PG   I L++ N+ EYV+ AL  R  E+  QV AVR+G++ + P  +L L  ++ +ES+VCG     + ILKK+ RYR++D ++ ++ W W+ LE+F+ND +ILF+ FVSGRSRLP +  D+SQRFQI++V+R  +GLPTAQTCFFQLRLPPY+S+E+M  RL YAI +CR+IDMD+YML+RN +
Sbjct: 3773 FAIGYSDGKVRMCVKDPY------DQDAIKTIEAHETSISGLTWDPTGHLLATLAEDVCLKLWGC-----NCDTWTMVYIIPHASPVTAMDWCPLLGKGDEPQLMLATGCSDGSVHVWTLPQPGST--VSIPFTSPRRTDQQAETRDRADTPPTSHQETIADESRQGPVEEVLVLKGHQTSIQHLAFHPHG-MGLASGCSR---GVVNLWSLQDGAVTQTLMGQGTTQGLVWAGEQALAAAFNKSKDVSLMYHSQDWFSQHTSLAAARMALRAQGIVGLHQAPCMKALLGRLPRILQEQYSYEKAHVVCGDQLVHSTFLQNFAAMAIGLQLEKVLCTVPCPPHLRNSVEEGCRNVPEWTWLASFSAALKSAETLSKRCAFPVSFVVPDI--ETISKDEPMQPTDNVAWKLNMDEEIMAWATQKPEDWMLGGRCEAYMWGGGRHGQLAETGRSLLTPTITPSFSQAQQIICGQNCTFVIQANGTVQACGEGSYGRLGQGNSDDLHQLTIISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEIIQMACGFKHSAVVSADGKLFTFGNGDYGRLGLGNTSNKKIPERVTALEGQMVGQVGCGLNHTLAVSADGNTVWSFGDGDYGKLGLGNSTAKSTPQRVDALCGIGVKKVCCGTQFSVALTKDGRVFTFGQERLIGQP-ESRSRNHNRPQQVPALEAFFVEDVVVGAEHSLALTSTGEVWAWGSNGDGQLGIGDTNARREPCLVTTLQGKSVRQISAGRNHSGGWTAPPPPKR----APGVPAPLQLGQPSSIPPQFTSL-KECSIDAIKARLRLLHHFSDLIYSSWRLLNLSPNQTMQVIPSRRQANLGVYSIGVEGLVQGDLRPLLAPRVYTLPMVRAIGRTMVQG---KNYGPQITVRRLATRGRKCKPIFHQIARQVVKLKPDELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGVIDLLLPTPNSSAEVGCNRDRFILNPSATTQDHLHQFKFLGVLFGVAIRTKKPLDLHLAPMVWKQLAGMPLTIDDIEDVDALFVQSLRSIRDIDKSGVTSETFHEVIPLDCFEGQSVDGRFVPIIPGGRSIPLTFHNRKEYVERALQYRLHEIDRQVSAVREGMAWIVPVTLLSLQTARHLESMVCGMPDISVDILKKVVRYREIDDNHHLVQWFWQTLEQFSNDERILFMRFVSGRSRLPANIADISQRFQIMKVDRTGDGLPTAQTCFFQLRLPPYSSQEVMAERLRYAINNCRSIDMDNYMLSRNAD 5050          

HSP 2 Score: 259.225 bits (661), Expect = 5.809e-65
Identity = 163/505 (32.28%), Postives = 264/505 (52.28%), Query Frame = 0

Query:  280 VNDVEKIETGSSCTYVIHTNGKV-SCWKDNHFS---------------NVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWG-------KTPWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNF--GLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
            V+ +  IE G  CT+V++ +G V +C K ++                 N      I   S S+ ++   LALT D +V+SWG+G +G+LGHG+     +PK+I        +  ISAG+ H+AA+T DG L+TWGEG  GRLGH D     +PTLVK++ N G V CG+ HT++VS+DG  VWSFG G+NG+LG GDT  +Y PK+I    G+ ++KV   ++ SLAL+ +G L+ WG        +P +T +  PK +  +++  I +V+ G  H + +  +  VY+WG N  G+CG G +   +  PK+   LD   +  I AG + S AWT     +    +H P+  +    TF+ +   L  +  G  D            E   F+L    LL ++ +  ++  + +    ++   L  ++F+ +D    +  I   V + + VGA  L P L++R+   ++LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  443 VSSILNIEAGQYCTFVVYNDGTVRACGKGSYGRLGLGDSNNQTTLKKLNFDHRYVIRKISSSKGSDGHTLALTADGEVFSWGDGDYGKLGHGNSSTQKYPKQIQGALQGKVVKSISAGYRHSAAVTEDGELFTWGEGDYGRLGHGDSNSKNLPTLVKDITNAGQVVCGSSHTIVVSQDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALVGLYIRKVCCGSQSSLALTSTGQLFAWGCGACLGCGSPEST-ALRPKLVEELANTRIVDVACGDSHCLALTHDNEVYAWGNNAMGQCGQGHSQSPITKPKKVVGLDGVTVHQISAGTSHSLAWTALPTDRQVVAWHRPFCVDLQESTFTHLRMFLERYCDGF-DSPNPPPPFPTSKEHHHFVLLCLKLLCTHLQLALAGGVATTILGSQARPLRHLLFRLMDSSTPN-SIQEAVSETLSVGAPLLLPPLRERMELLHQLLPQGPARWESLSKGQRMQLGIILTSLQDNTHVASLL 944          

HSP 3 Score: 163.31 bits (412), Expect = 6.148e-36
Identity = 113/389 (29.05%), Postives = 186/389 (47.81%), Query Frame = 0

Query:  247 MYLTEQIVHFTSRNKQD--------IYTWGCDLH-----------SPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVH----------FPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGK-----TPWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWT 582
            + + E+I+ + ++  +D         Y WG   H           +P I    +  ++I  G +CT+VI  NG V    +  +         ++H          F    +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    I+ ++ G  H+A ++ DG L+T+G G  GRLG  +     +P  V  L    VG V CG  HTL VS DG  VWSFG G+ G+LG G++    TP+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P + S     P+ + ++ +  + +V  G  H + +     V++WG N  G+ G+G T+ + + P     L    +  I AG   S  WT
Sbjct: 4163 LNMDEEIMAWATQKPEDWMLGGRCEAYMWGGGRHGQLAETGRSLLTPTITPSFSQAQQIICGQNCTFVIQANGTVQACGEGSYGRLGQGNSDDLHQLTIISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEIIQMACGFKHSAVVSADGKLFTFGNGDYGRLGLGNTSNKKIPERVTALEGQMVGQVGCGLNHTLAVSADGNTVWSFGDGDYGKLGLGNSTAKSTPQRVDALCGIGVKKVCCGTQFSVALTKDGRVFTFGQERLIGQPESRSRNHNRPQQVPALEAFFVEDVVVGAEHSLALTSTGEVWAWGSNGDGQLGIGDTNAR-REPCLVTTLQGKSVRQISAGRNHSGGWT 4548          

HSP 4 Score: 162.155 bits (409), Expect = 1.470e-35
Identity = 125/376 (33.24%), Postives = 177/376 (47.07%), Query Frame = 0

Query: 2544 SSVYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVI------CGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPK-IISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI------------------------NFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            S VY+ G+  + QLA+     I  PK + +F  + +V        G   TF +  +G++ ACG+GSYG+LGLG+S++  +L         V+  + S      H L       VFSWGDG+ GKLGHGN+   + PK I  +L G     ++ G  HSA +T+ G++FT+G GD GRLGHG+  +K  P  +  +       V CG  HT +                        N  ++  V          K+C G   SL LTS G +F  G     G    S       PK + +L N  +VD+A G  H LAL+    V+ WG N +GQ G   S     +P  +  L G  + QISAG +HS AWT+ P
Sbjct:  407 SEVYVWGSNSSHQLAEGSQEKILQPKLSPAFANAQTVSSILNIEAGQYCTFVVYNDGTVRACGKGSYGRLGLGDSNNQTTLKKLNFDHRYVIRKISSSKGSDGHTLALTADGEVFSWGDGDYGKLGHGNSSTQKYPKQIQGALQGKVVKSISAGYRHSAAVTEDGELFTWGEGDYGRLGHGDSNSKNLPTLVKDITN--AGQVVCGSSHTIVVSQDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALVGLYIRKVCCGSQSSLALTSTGQLFAWGCGACLG--CGSPESTALRPKLVEELANTRIVDVACGDSHCLALTHDNEVYAWGNNAMGQCGQGHSQSPITKPKKVVGLDGVTVHQISAGTSHSLAWTALP 778          

HSP 5 Score: 139.428 bits (350), Expect = 1.082e-28
Identity = 161/548 (29.38%), Postives = 253/548 (46.17%), Query Frame = 0

Query:   86 NPEEDTCRSESLQNEALLNLGLQTGLSLLFSLLQQNW--SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLH-----TSAQIPQASTSCYELLLSLAIQRGRCSDVLHWIN--------TALTVAIQNPSL-TINPVIFNKAVQLAQG--RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQDIYTWGCDLHSPKIWDDVNDVEKIETGSSCTYVIHT--NGKVSCWKDNHFSNVHFPSPI-IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPK------EISSLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLH--------NVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLI-NFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISSISNVSTGGFHLIVILEN-RSVYSWGLNTYGKCGLGLTSLKVKNPKRLD----IYDIDGICAGPTQSYAWT 582
            N E+ +       N+AL+ +G++TGLSLLF+LL+Q+W  S P     L + VI TA +++ S PPLSL     +PP+G   L  V  FL      TS           ELLL LA QRG    +L WI             V  +   L  I+   F + +Q+ +   R ++    +  E  MYLT      TS   + I   G    + K    + D+ K   G    Y+       +V+    ++  +   PSP+ ID S + +N + +   TE+ +VY WG+ S  +L  G ++    PK         ++  I+ I AG +    +  DG +   G+GS GRLG  D       T +K L+         + S      HTL ++ DG +V+S+G G+ G+LG G++     PK I     G ++K + A  R S A++  G+L+ WG+  +       + S  +P  +  I++   V  G  H IV+ ++ R+V+S+G    GK G G T+ +V  PK ++    +Y I  +C G   S A T
Sbjct:  140 NSEKSSGEKTRTGNDALIEMGVRTGLSLLFALLRQSWLQSPPGGNLSLCNDVIQTAMDVVSSLPPLSLANETKMPPMGLDCLKQVTEFLKSVTMPTSGADSLGKRLAAELLLGLASQRGSLRYLLDWIEMALAASAALGSQVEREEDRLGQISHEFFQQILQVMENSVRRHEEECGHSIE-RMYLT----RMTSLQPKGISQGGSG--NDKRDHQITDMNKTGDGMFPLYLAGMVLMEEVAWLASDYARSCVCPSPVPIDDSSTGENGNMV---TENSEVYVWGSNSSHQLAEGSQEKILQPKLSPAFANAQTVSSILNIEAGQYCTFVVYNDGTVRACGKGSYGRLGLGDSNNQ---TTLKKLNFDHRYVIRKISSSKGSDGHTLALTADG-EVFSWGDGDYGKLGHGNSSTQKYPKQIQGALQGKVVKSISAGYRHSAAVTEDGELFTWGEGDYGRLGHGDSNSKNLPTLVKDITNAGQVVCGSSHTIVVSQDGRTVWSFGGGDNGKLGHGDTN-RVYKPKVIEALVGLY-IRKVCCGSQSSLALT 671          

HSP 6 Score: 95.1301 bits (235), Expect = 2.480e-15
Identity = 74/243 (30.45%), Postives = 126/243 (51.85%), Query Frame = 0

Query: 1228 EHIVRKLFSHIYNSYWNSKSSDLDFTNIQRTSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINN---RSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSC 1464
            E I +KL  H  N    S  +  +  N+     N  F+ + +  C +++  T+V      GY + +  IS G   WK  I+K+++G +   +GV+   + +   R+  DMWLY + SG L H  G  S  LPSF++GD I  +L+ E  ++SF KNG    LAF D+  S EL+PVV F S+    K+ I+++ +++ +   L   +P         ++  V++IR+L+  + + W   I++C
Sbjct: 1954 EEIDKKLLKH-QNPDAGSPDAGWEEENMAVQEAN--FDPERTVCCSVENGHTLVHGAGGRGYGLGSTGISSGCWQWKFLIIKENRGNEGTCVGVSKFPVQDFSHRTTADMWLYRAYSGNLYHN-GEQSLALPSFTQGDYITVVLDMEARTVSFGKNGEEPRLAFEDV-DSTELFPVVMFYSSNPGEKVKITDM-QVRGAPRDLLPGDPTCAPAPAVTSEAAVQLIRTLH--RQEGWTGHINNC 2188          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|1080051068|ref|XP_018567016.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Anoplophora glabripennis])

HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0
Identity = 483/1269 (38.06%), Postives = 717/1269 (56.50%), Query Frame = 0

Query: 2167 LATGSCDGTIRL-WKDNEYKRILIIDFDDESTLEISVMSDQILTWSPSGKYLV-LSVGNIIYIWDMEDSE-ENCNQIPQLSPIIYLCWSP---NSEIEILLAARQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITC--LHWIN-SKTLAVGFCNTENVTLMN--LPNEMDNVIIASKCRAELFEHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLC-----------SLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVNLKD-IVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLL-------------SHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT-------------------KINFAWRES--------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVD-----IAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDS--DFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEED---SLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE 3348
             A G  DGTI+L W  +E   I         TL+    +   L+W P G +L  +S+     IW+++D +  + + + Q    I L WSP   +S   +LLA   N  ++++ T           I  EN E +V ++    +  H Y S + S  +   S    LASG  K P G+VNIWS+   S+++T+ G GGI    + W++ S  LA+GF  ++++ ++N  + +   N+ +A+   + + + +   +  P+F+ F+      ++EQY  EK+ +  G  L++S +L++L+ LA +L L+ ++C           S     + WL +F+ +      L KR        L+ +  + I+  V   D + WT + D  +M+W    P DWQ GGK   +L G+ ++GQLA+LG SS  P + +SF  S  ++CG   TF ++ NG++ ACGEGSYG+LG GNSDD  +L+ + +L                H+L       VFSWGDG+ GKLGHGN++R RRP+ I +L       VA G  HSAV+T  GK+FTFGNGD GRLG G+   KK PE+++SL G+ +  VACGL HT                   K+      +              + K+  G   ++FLT SG +F CG  +        S++  ++  +  +L  +VD      AVG +HVL L+  G V+GWG N   QLG       +EP II ELS KG+ QIS G+ HSAAWT+ P+P      + +    + G P  +P +F   L   S     +RLKLL+  S+ + + W L P+ D   D   PP        + +  +R  L+P V TLP ++ +  TM      +NYGPQ+ V+R+   G     IF QIA QV  + P+ELRLP R+WKVKL+GE ADDAGGVFDDTI+EMCQE++  V+ LL PTPN I+D G N+DKYLLN    S  ++  F FLG+L GVA+RTRKP+A+++APL+WKLI+    + +DLED D  Y +SL  +         E+    ++P + F G S +G+ VP+  G  KI L+YEN+ +Y + A+  R +E   QV A+R+G+S + P P+L L+ ++ +E LVCG +   + +LKKI RYR++D  ++++ WLW+ILE F ++ ++LF+ FVSGRSRLP +  D+SQRFQI++V++P NGLPTAQTCFFQLRLP YT++EIM  +L Y+I +CR+IDMD+YML RN +
Sbjct: 3132 FAIGYLDGTIKLGWAHSESNVI---------TLKAHESAVSSLSWDPRGIFLATISIDLTCKIWNLDDDKLVHVHTLAQTYEPISLTWSPLVGSSNEALLLAVGSNFGTVNVWT-----------IPKENEENIVPKLIMNSQG-HAYNS-VTSITI--DSTGLFLASGCLKGPSGVVNIWSLHDGSLVQTVAGSGGINASGMCWLSTSNALAIGFSRSKSICVLNCSVQDLSKNLPLAAVRCSLIRKGIRDLKSVPFFKLFVTLLPNILLEQYNTEKMAVQTGTHLMHSMYLKSLASLAILLELDKIVCYKLCPFNNKGQSELVPEYLWLHTFSLANQMADSLIKRTKS-----LEIVSLNHIQGVVKCPDNVFWTMKQDQQIMQWVSQRPQDWQIGGKCRAFLWGSDRHGQLAELGYSSSVPTHVESFSASRKIVCGQNCTFVIEANGTVLACGEGSYGRLGQGNSDDLHTLSVISSLQGFVIIDLATSVGSDGHSLALAESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQNEEVIQVACGFKHSAVVTSDGKLFTFGNGDYGRLGLGSTSNKKVPERVTSLDGFKIGQVACGLNHTACMSVDGMNVWTFGEGDYGKLGLGHTATKSIPQLVETMCNIGIKKVGCGTNLTVFLTKSGKIFVCGVDRVPW----QSHLRERTDYRPQQLMGLVDYFIEDFAVGTEHVLFLTSCGKVFGWGMNTESQLGLPHVSLVREPEIIMELSDKGVKQISTGRTHSAAWTASPLPLRIPGVTCSL---SFGLPQEIPPQF-DHLQGLSVPSIQSRLKLLYNFSDKLYSCWTLMPLSDEQIDMKIPP-----LEGLISPKLRYLLAPRVYTLPFVRCIGKTMIQG---KNYGPQIIVKRISQEGQKCKPIFVQIARQVVDLKPQELRLPSRAWKVKLLGEGADDAGGVFDDTITEMCQEITTGVVPLLIPTPNAINDEGYNRDKYLLNPQLLSTQNLNWFKFLGILFGVAIRTRKPLALSIAPLIWKLIVGEPVSIEDLEDTDCMYIQSLRSIRDIHLCGVTEEYFHDVIPLETFEGTSCTGKVVPIIHGGHKILLTYENRMQYYEQAIKFRMQEFDIQVAAIREGMSGIIPVPLLSLVTAEHLEKLVCGMAHISISVLKKIVRYRELDEDHQLVQWLWDILENFTDNERVLFMRFVSGRSRLPANLADLSQRFQIMKVDKPVNGLPTAQTCFFQLRLPSYTTQEIMAEKLRYSINNCRSIDMDNYMLARNTD 4355          

HSP 2 Score: 322.783 bits (826), Expect = 3.637e-84
Identity = 256/782 (32.74%), Postives = 386/782 (49.36%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNW--SGPDEQHPLSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFL-----HTSAQIPQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIFNKAV-QLAQGRTY-------DSHTIYLKEAAMYLTEQIV--------------------HFTSRNKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVS-CWKDNH-------FSNVHFPSPIIDASL------SQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTP-WNTSSTVPKWINSI-------SSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTMRKGKGCF---HWPYSNEPDAKTFSQVSDILYNFGLS-DFNAKLNSHFDEDEQQKFLLHIFTLLISYFET-ISSSIRSCDWSAEKVKLEDIIFKFIDKENISP-EIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-KMNNLSKGQQYKLKMDLESLNEPGIISGLICES---EEGKDAAKKLLQHLVYKMHHDFVQSLFECDSKMFDLEMSVGYSIHWQ 792
            + ALL +G++TGLSLLFSLLQQNW  S       L ++V+ +  +++   PPLSL     +  LG  +LD V  FL     + +A   Q      E+LL +A+QRG    +L WI TAL  + + P+L+     F +A+ Q+             +   I   +A  YL E +V                          K D+Y WG +            +  P   +  N V+++E G  CT+ IH +G VS C K ++        SN   P PI+  S+      S+ ++   LALTED  VYSWG+G +G+LGHG+   +  P+ I+       I  I+AG+ H+AAIT DG LYTWGEG  GRLGH D     +PT V +L +VGSV+CG+ HTL+VS+DG  VWSFG G NG+LG G+   +Y PK+I    G++++KV A   FS+AL+  G ++ WG  P   T S    W+  +         I ++S G  H + + +   V++WG N+ G+CG G TS  +  P +   LD   I  I AG + S AWT +  +  +   H P+  +   KTF  +   L  +  S  +N+        DE ++F+L    LL  +    IS ++ +        +L  ++F+ IDK+  +P E++  V + +  GA  L P L +RV   +  L    NLS GQQ  L + L SL EP + + L+  S   E+ K    +L   L+  +   F ++  E  S +++  +S      WQ
Sbjct:  153 SHALLEIGVKTGLSLLFSLLQQNWQVSTMLSIPSLCNSVLESTVDLIQKLPPLSLSNDSQLTNLGCNSLDQVCGFLKNAILNETAADNQGKILAAEILLEIALQRGSLHYLLEWIETALEASCKGPNLSSKQ--FKRAIIQIGAKNNLGLELLKNEDDEISTYQAGYYLIEVLVSMALDCIGTCSAAGSLSCESDLAPFEKSDVYVWGSNSSHQLAEGNQEKILLPIKSNIFNQVQQVEAGHYCTFAIHWDGHVSACGKGSYGRLGLGESSNQSLPKPILLDSIVKKLSSSKGSDGHTLALTEDGMVYSWGDGDYGKLGHGNCATHKQPERITGPFLGKNIKYINAGYRHSAAITEDGLLYTWGEGDHGRLGHGDTNERHIPTQVADLTDVGSVACGSSHTLVVSRDGKTVWSFGSGENGKLGHGEIAKVYRPKVIEALQGLVIQKVCAGTSFSMALTTMGHVYTWGSGPILGTGSADAIWLQPLLLDDLTPYRIVDISAGDTHCLALTDENVVFAWGTNSMGQCGQGHTSSPIMRPLKVIGLDGIPIRQISAGTSHSLAWTSKPKENQYITKHRPFCLDLHDKTFELLKIFLEKYTTSFTYNSPPLPFASPDEHKRFVLCCLKLLYIHLNICISGNLDTKVLKQHSKELRILLFRLIDKD--APLEVHNSVREVLNTGAALLLPPLVERVEFLHLYLSNGKNLSVGQQMLLGIILHSLEEPVLTAALLGYSSSPEKLKLQEIQLTSTLMKTLLQSFCKNTEETLSTIWN-HVSANTKCKWQ 929          

HSP 3 Score: 173.711 bits (439), Expect = 4.204e-39
Identity = 126/382 (32.98%), Postives = 180/382 (47.12%), Query Frame = 0

Query: 2536 SDWQPGGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV-----------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRP-KIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGN-ESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNF 2861
            SD  P  KS VY+ G+  + QLA+     I  P  ++ F     V  G   TFA+  +G + ACG+GSYG+LGLG S +     P++                 TL L+ + + V+SWGDG+ GKLGHGN    ++P +I     G    ++  G  HSA +T+ G ++T+G GD GRLGHG+   +  P Q++ L    V  VACG  HT                        +I   +R  V          K+C G +FS+ LT+ G V+  G+    G    S++     P  +  L    +VDI+ G  H LAL+    V+ WG N +GQ G   +      P+ +  L G  I QISAG +HS AWTS P    +
Sbjct:  344 SDLAPFEKSDVYVWGSNSSHQLAEGNQEKILLPIKSNIFNQVQQVEAGHYCTFAIHWDGHVSACGKGSYGRLGLGESSNQSLPKPILLDSIVKKLSSSKGSDGHTLALTEDGM-VYSWGDGDYGKLGHGNCATHKQPERITGPFLGKNIKYINAGYRHSAAITEDGLLYTWGEGDHGRLGHGDTNERHIPTQVADLTD--VGSVACGSSHTLVVSRDGKTVWSFGSGENGKLGHGEIAKVYRPKVIEALQGLVIQKVCAGTSFSMALTTMGHVYTWGSGPILGT--GSADAIWLQPLLLDDLTPYRIVDISAGDTHCLALTDENVVFAWGTNSMGQCGQGHTSSPIMRPLKVIGLDGIPIRQISAGTSHSLAWTSKPKENQY 720          

HSP 4 Score: 167.162 bits (422), Expect = 4.559e-37
Identity = 116/388 (29.90%), Postives = 183/388 (47.16%), Query Frame = 0

Query:  246 AMYLTEQIVHFTSRNKQD--------IYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI--------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN--VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWG--KTPWNT-----SSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWT 582
             M   +QI+ + S+  QD         + WG D H            P   +  +   KI  G +CT+VI  NG V    +  +         ++H  S I        ID + S  ++   LAL E  +V+SWG+G +G+LGHG+      P++I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG        VP  V +L    +G V+CG  HT  +S DGM VW+FG G+ G+LG G T     P+L+     + +KKV      ++ L+ SG +++ G  + PW +     +   P+ +  +    I + + G  H++ +     V+ WG+NT  + GL   SL V+ P+    L    +  I  G T S AWT
Sbjct: 3479 TMKQDQQIMQWVSQRPQDWQIGGKCRAFLWGSDRHGQLAELGYSSSVPTHVESFSASRKIVCGQNCTFVIEANGTVLACGEGSYGRLGQGNSDDLHTLSVISSLQGFVIIDLATSVGSDGHSLALAESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQNEEVIQVACGFKHSAVVTSDGKLFTFGNGDYGRLGLGSTSNKKVPERVTSLDGFKIGQVACGLNHTACMSVDGMNVWTFGEGDYGKLGLGHTATKSIPQLVETMCNIGIKKVGCGTNLTVFLTKSGKIFVCGVDRVPWQSHLRERTDYRPQQLMGLVDYFIEDFAVGTEHVLFLTSCGKVFGWGMNTESQLGLPHVSL-VREPEIIMELSDKGVKQISTGRTHSAAWT 3865          

HSP 5 Score: 130.568 bits (327), Expect = 4.416e-26
Identity = 144/605 (23.80%), Postives = 270/605 (44.63%), Query Frame = 0

Query: 1227 REHIVRKLFSHIYNSYWNSKS------SDLDFTNIQRTS------------YNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINN---RSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSS---------LISQSEKLRG--SIDNVLEAIYPLLALLSDVDSGLCLGSYVRYKEF-KRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSY---------------KSEEERNKNKENPASTS--------------QAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVF------------------FKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNIS-----FSRLINRSEIERTFSILN 1743
            R++I+ KLF  +    WN  +      SD  F N++ T             ++M F+ +  Y+C ++ + T+V      GY +  + I  G   WKI I+K+++G +   IGV+   + +   RS  DMWLY + SG L H  G       S+++GD I  IL+ +  +LSF KNG    +AF ++ +S ELYP V F ST    K+ I+++ ++  +   L   EP L  L   +++  + +IR L++  S  W   +++     +    E ++ L     +          ++++ KL+   S+  +   ++P L ++  +D GL +G Y ++K   +R + +G+       V ++WE+DGS S  ++  L+ I+ +          K+++    +LL I   S   N + F +Y               KSE   + + +N   +S              + VE++T+ +VSSI+ EV   S      +  E  I++++      EV                    ++  LQF+++K +     +     S  + N+    E +  IR +     + S       ++++ SE+ER  ++L+
Sbjct: 1693 RKYIINKLFIQLGQEMWNIPNPSEKPISDTKFKNVRPTDTSVREDEGNIPIHDMGFDAEKCYNCSIEGSLTLVHGTGGRGYGLGLQVIKSGCYQWKILIVKENRGNEGTCIGVSKYPVKDFSHRSTSDMWLYRAYSGSLYHN-GERDMSFQSYTQGDYITVILDMDAKTLSFGKNGEEPRVAFENIEAS-ELYPCVMFYSTNPGEKVKITDM-KVHGTQKDLLPGEPELAPLHAVLSEAYISVIRRLHH--SVTWTEDVNTA----LFTRLEKIETLFPIVETHNMDNTSELKLNKTYKLKCDISVPELCVNVWPCLIVIGGLDRGLRMGGYCKHKNTGQRAVALGILKKGITTVNVQWENDGSVSDVALSNLEFIEPV-----PFDCTKFNAVTPKILLQIARLSGITNETSFPTYDLTDDEQLLLKQHILKSENGNSLDSKNIRCSSDSQIHDQYITHEKPKTVESLTNEMVSSIMGEVKRLSTEKIVASQSESTIKETNELHNKQEVITSETKMLRKKLLDLEGECLQLAFLQFAALKVLGIFLISSPLTESLLINNKESKTEDIDCIREIMHSIVDKSIDQCKLRKIVSISEMERAQTVLH 2283          

HSP 6 Score: 73.559 bits (179), Expect = 9.345e-9
Identity = 65/256 (25.39%), Postives = 117/256 (45.70%), Query Frame = 0

Query: 2076 DKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFK----NHSDILGALPEDIYSYQLSGHAHKIVSMT--SYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQI-----------LTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEIEILLAARQNGTSISITTLSWSSN-------SDYLAIGYENGEILVLQIDQRKESIHL 2307
            D+ NI P  +++ALS+ ILSQ++  ++     K WAA  I K +   +  + + G      N +D+ G LP    +Y + GH +++ ++     +  LA+   DGT+RLW     K+ ++     ESTL     +D             + WSP+G+Y+  ++ N + IW ++ SE+               +S  S+  I      +     IT+++W  N        DYL IG  NG ++++ + +    + L
Sbjct: 2889 DRNNIGPLTLVNALSAYILSQKVDQIN-----KEWAAHQIYKSLATKI--QIMAGVNLDQTNFADLSGTLPMQSLTY-VDGHDNRVSTLAWLDRSGSLASAGYDGTVRLWTVEPSKQPIL-----ESTLVFHTSADFFGNDLQGRLIGHMKWSPTGEYIAAALDNFVNIWHLKKSEQ---------------FSSYSDRFI------DDQKEFITSMTWPRNYNGCKHSEDYLLIGKINGSVVMVTLYEETRRVEL 3110          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|939665098|ref|XP_014279948.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Halyomorpha halys])

HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0
Identity = 473/1156 (40.92%), Postives = 651/1156 (56.31%), Query Frame = 0

Query: 2284 LAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPY----------ILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNEMDNVIIASKCRAELFEHLPFE-QFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLC---SLPDSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEI---KKDVNLKDI--------VWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLT--------PVVTLLLS-----HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSL----LPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYW---PPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS---IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVA-TSFNPDNEED--SLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            LA+G  NG I V  I    E +     +I+  L  H  +P           IL SG  K   G++N+WS+L   VI+T  G GG+  L W++S  LAV F  +++++++        + IA   R  L +   F    AP   AFI+     + +QY YE   +S G  LIYS +L+ L  LA  L+    LC   S P+  ++  +SF    +++ L+++    S   + ++ F  E    K ++   +         VWT +AD  +M W    P DWQ G K + YL GNG++GQLA++G SS  P   +SF  +  +ICG   TF +Q NG + +CGEGSYG+LG G++DD  SL+         +V L  S     H+L       VFSWGDG+ GKLGHGN +R RRP+ I +L G     VA G  HSAV+T  GK+FTFGNGD GRLGHGN   KK PE+++S  G  +  VACGL HT                                 KI   +RE + K+C G  F++FLT+ G VF CG  +  G+   S       P+ +  L    + DIA G +H LALS  G V+GWG N   QLG   S    K P +I  LS K I QIS G+ HSAAWT PP+P      S        G P S+P ++  L    +P   A     RL LL+  S+ +   WR  P+   +  W   PP     Y+ + +  +R  LSP V TLPL++ +  TM      RNYGPQVT RRL      +   IF+Q+A Q+  +DP +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMC+E+    + LL PTPN I+D G ++D+YLLN        +  F FLG+L GVA+RT+KP+A+ L+ LVWKL+++   +W DLE+ D  Y++SL  +     +   EE+   ++P + F G S++G+ VP+ PG   I L++ N+ +YV+ A+N R  EM  Q+ AVR+G+S + P P+L L+ +  +E LVCG     + +LKKI RYRD+D  N +I WLW  LE F +  K+LF+ FVSGRSRLP +  DVSQRFQ+++V+R  +GLPTAQTCFFQLRLPPY+S E++ +RL YAI  CR+IDMD+YMLTRN
Sbjct: 3226 LAVGTINGMIYVWLISLPNEVVE---PRILHSLQGHLYNPIKTLSINSEGTILVSGCGKGTSGVLNMWSLLDGCVIQTHTGSGGVQSLAWMSSIGLAVAFARSKDISIIRCSRASSILPIA---RHNLMKRGVFGLHTAPCLTAFIENLPVHMFKQYNYENPYVSSGKHLIYSDYLKCLVALALSLSFEKALCYNRSPPN--YEDSTSFVPEASWLRLVSQAAEASKALICRTTFPSEFLLHKPEIAQPEYWPMACDNEVWTLKADEEIMSWATGLPQDWQLGSKCNAYLWGNGRHGQLAEIGHSSPKPVLCESFSNAQQIICGQNCTFVVQANGVVLSCGEGSYGRLGQGHADDLHSLSIISSLQGFVIVQLATSCGSDGHSLALAESGEVFSWGDGDYGKLGHGNGDRQRRPRQIEALQGEDVVQVACGFKHSAVVTSDGKLFTFGNGDYGRLGHGNTLNKKLPERVASFHGIPIGQVACGLNHTVCVSIIGTMCWSFGEGDFGKLGLGNTSTYHTPQKIEALYREVIKKVCCGAQFTVFLTADGRVFTCGMERLIGLP-ESRYSGSNRPQLVSSLSPHFIQDIACGAEHTLALSSEGLVFGWGTNSDAQLGLGHSALSVKLPALIAILSDKNIRQISTGRTHSAAWTVPPVPRRIPGVSRGA---QFGMPQSVPSQYGHLQNFPIPAIQA-----RLSLLYHFSDSLFQCWRFLPLCAQESVWAQIPP-----YVWLGSPQLRALLSPRVYTLPLVRCIGRTMVQG---RNYGPQVTARRLATRKCPAVDPIFTQVASQIVKMDPADLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCEEMLSGAVPLLVPTPNTINDTGYSRDRYLLNPALTDPHHLTWFKFLGILFGVAIRTKKPVALPLSTLVWKLLVKEIVSWDDLEENDALYAQSLRGIRDIHLSGVTEENFHEVIPLECFEGTSWNGKMVPIVPGGRSIPLTFMNRLQYVEQAVNFRLHEMDLQISAVREGMSWIIPVPLLHLITASHMELLVCGLPHISIPLLKKIVRYRDLDAGNPLIQWLWSALESFTDAEKVLFMRFVSGRSRLPANLADVSQRFQVMKVDRAVDGLPTAQTCFFQLRLPPYSSPEMLADRLRYAINCCRSIDMDNYMLTRN 4356          

HSP 2 Score: 315.079 bits (806), Expect = 7.032e-82
Identity = 240/730 (32.88%), Postives = 369/730 (50.55%), Query Frame = 0

Query:   93 RSESLQNEALLNLGLQTGLSLLFSLLQQNW---SGPDEQHP-LSSTVISTANNMLLSFPPLSLLA---VPPLGETALDNVATFLHTSAQIPQASTS-----CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPVIF----NKAVQLAQGRTYDSHTIYLKEAAMYLTEQIVHFTSR------------------NKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPSPIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLP--PIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIW------GKTPWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIYDIDGIC---AGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHFD---------EDEQQKFLLHIFTLLISYFET--ISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKM-NNLSKGQQYKLKMDLESLNE 734
            R     + ALL + ++TGLSLLF+LL+Q+W     PD   P L + V+ TA +++ S P LSL +   +  LG   L  V +FL ++A     +T      C ELLL+LA QRG    +L WI+ AL  +     +  N   F     K V L +    D + I L +AAM + + IV+                      +K +++ WG +            +  PK     ++V ++E G  CT+V+H NG+V +C K ++                V F S I   S S+ ++   LAL+ED +VYSWG+G +G+LGHG+      PK I       ++ ISAG  H+AA+T DG+LYTWGEG  GRLG  D + H VPTLV +L  VGSVSCG  HTL++S DG  VWSFG G  G LG GDT  +  PK+I+   G+ ++KV+A + FS+AL+ +G +  W      G    ++  T+P  I  ++S  + +++ G  +++ +  +  +++WG NT G+CGLG  +  V  P+R+   D   IC   AG + + AWT     +    +H P+  E    TF+          L  F  K +SHFD         + E ++F +    LL ++     I+ S  +  + A+   L   +F+ +D   +   +   V +C+ VGA  L P L++R+   + LL   ++LSKGQ+  L + + SL E
Sbjct:  174 RQHVTGSHALLEVAVKTGLSLLFALLRQSWYYSQIPDGPQPNLCNQVLRTALDVVKSLPVLSLASESQLSTLGLETLTQVCSFLESTADPKSGATKSDRKLCCELLLALAAQRGSLPYLLEWISMALQCSASESCIDKNLFFFVLSSMKPVSLPKFDGPDDN-IPLYKAAMLMMQVIVNLACDYTMSWGITQAGEINQNELDKCEVFVWGSNSSHQLAEEKTEKIPFPKKSTAFSNVRQVEAGQYCTFVVHNNGEVTACGKGSYGRLGLGDSDDQQTLKRVIFDSRIKKISSSKGSDGHSLALSEDGQVYSWGDGDYGKLGHGNSTTQRQPKMIGGHGWWNVVSISAGFRHSAAVTTDGFLYTWGEGEFGRLGIGDNVSHSVPTLVPDLSGVGSVSCGNTHTLVLSMDGKTVWSFGSGEGGRLGHGDTNNVPRPKVIDALVGLYLRKVVAGSLFSIALTCNGSVLAWGVGSCLGTGSSDSMRTIPTPIEDLASTHVVDIAVGDTYVLALTHDSGLFAWGNNTMGQCGLGYLTSPVTRPRRVSSLDTVSICQISAGTSHAIAWTAPPTDRQSVAWHCPFCVELKEGTFAL---------LLAFLEKYSSHFDGNGQEPFCNKHEHEEFAVLCLRLLCAHLSLSHIAGSEPTSIFGAQANPLRRYLFRLVDIP-LPKSVSMIVTECLAVGASLLLPPLRERLELLHSLLPYGSHLSKGQKMLLSIIVTSLEE 892          

HSP 3 Score: 173.326 bits (438), Expect = 5.591e-39
Identity = 112/367 (30.52%), Postives = 185/367 (50.41%), Query Frame = 0

Query:  260 NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWK-------DNHFSNVHFPS--------PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNV--GSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWG-------KTPWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKRLDIY---DIDGICAGPTQSYAWTM 583
            +K + Y WG   H            P + +  ++ ++I  G +CT+V+  NG V SC +         H  ++H  S         I+  + S  ++   LAL E  +V+SWG+G +G+LGHG+      P++I +L    ++ ++ G  H+A +T DG L+T+G G  GRLGH + +   +P  V + H +  G V+CG  HT+ VS  G   WSFG G+ G+LG G+T   +TP+ I      ++KKV    +F++ L+  G ++  G            + S  P+ ++S+S   I +++ G  H + +     V+ WG N+  + GLG ++L VK P  + I    +I  I  G T S AWT+
Sbjct: 3501 SKCNAYLWGNGRHGQLAEIGHSSPKPVLCESFSNAQQIICGQNCTFVVQANGVVLSCGEGSYGRLGQGHADDLHSLSIISSLQGFVIVQLATSCGSDGHSLALAESGEVFSWGDGDYGKLGHGNGDRQRRPRQIEALQGEDVVQVACGFKHSAVVTSDGKLFTFGNGDYGRLGHGNTLNKKLPERVASFHGIPIGQVACGLNHTVCVSIIGTMCWSFGEGDFGKLGLGNTSTYHTPQKIEALYREVIKKVCCGAQFTVFLTADGRVFTCGMERLIGLPESRYSGSNRPQLVSSLSPHFIQDIACGAEHTLALSSEGLVFGWGTNSDAQLGLGHSALSVKLPALIAILSDKNIRQISTGRTHSAAWTV 3867          

HSP 4 Score: 168.703 bits (426), Expect = 1.275e-37
Identity = 123/369 (33.33%), Postives = 176/369 (47.70%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV-----------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKI---------NFAWRES------------------------VTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYF---YKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            K  V++ G+  + QLA+  +  IP PK + +F     V  G   TF +  NG + ACG+GSYG+LGLG+SDD  +L  V+                 +L LS +   V+SWGDG+ GKLGHGN+   R+PK+I          ++ G  HSA +T  G ++T+G G+ GRLG G+  +   P  +  L G  V  V+CG  HT +         +F   E                         + K+  G  FS+ LT +G V   G     G   + S   I +P +     ++VDIAVG  +VLAL+    ++ WG N +GQ G   GY       P  +  L    I QISAG +H+ AWT+PP
Sbjct:  375 KCEVFVWGSNSSHQLAEEKTEKIPFPKKSTAFSNVRQVEAGQYCTFVVHNNGEVTACGKGSYGRLGLGDSDDQQTLKRVIFDSRIKKISSSKGSDGHSLALSED-GQVYSWGDGDYGKLGHGNSTTQRQPKMIGGHGWWNVVSISAGFRHSAAVTTDGFLYTWGEGEFGRLGIGDNVSHSVPTLVPDLSG--VGSVSCGNTHTLVLSMDGKTVWSFGSGEGGRLGHGDTNNVPRPKVIDALVGLYLRKVVAGSLFSIALTCNGSVLAWGVGSCLGTGSSDSMRTIPTPIEDLASTHVVDIAVGDTYVLALTHDSGLFAWGNNTMGQCG--LGYLTSPVTRPRRVSSLDTVSICQISAGTSHAIAWTAPP 738          

HSP 5 Score: 124.79 bits (312), Expect = 3.067e-24
Identity = 128/500 (25.60%), Postives = 223/500 (44.60%), Query Frame = 0

Query: 1226 NREHIVRKLFSHIYNSYWNS-----------KSSDLDFTNIQR------------------TSYNMEFNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGIINN---RSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDL---------------------LKXFSSLISQSEKLRGSI-------DNVLEAIYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWED-DGSSSFRSIQCLKLIKKIDSPVHK-------IFMY-----KYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSES 1648
            ++E ++R LF  +  + W             K  DLD T ++R                      + F+ +    C ++ T  ++  G+  GY +AN  I+ G   WK  ILK+ KG +   IG+    + +   R+  DMWLY + SG L H  G     LPSF++GD+I  +L+ +  ++SF KNG   ++AF D+  +  LYP V F ST    K+ I ++ ++  +   L   +P    + + +A+  + +IR+L+N  +  W S I+ C    + ++ E++ +L                     LK  +   S SE     +       D + + ++P L ++  +D GL +G    +K+  R   I+G       NVKI W D D + S   +  L+    +     K       I++Y      ++SE I++  +  T SE   L V S    E ER   K      +++VE +++ +V++II EVT +  +
Sbjct: 1703 DKEQVIRVLFRQLSANMWTIPQILAERQAYIKEMDLD-TQLERLMSPGGASLLQGSSDDCLPVMEIGFDPEKCVSCTVE-TGNILVHGQGRGYGLANVSITSGCYQWKYLILKEHKGNEGTCIGLAKCPLKDFSHRTTSDMWLYRAYSGNLYHG-GELPLCLPSFTQGDVITAVLDLDSRTISFGKNGDEPIIAFQDIDPATALYPCVMFYSTNPGEKVEIFDM-QMSGAPRELLAGDPQCAPVPVLLAEAYIHLIRTLHN--TDFWCSQINECLIERLNQAKEILPELKIDLLPEDEKEQNLHEPVASDLKLKNETESNSEFHEDMVVDKEIDYDQLCKEVWPALVVIGGLDHGLRVGGLCLHKQSGRKATILGTLKQGMTNVKIMWHDADYAISDCPLTSLEPFNAVTFDAAKLPYIPPEIWLYMARLSGFTSE-IELPEMNLTQSE---LEVISDVNPETERPAEKNT--YFTRSVETLSNEMVTTIIGEVTRRGST 2190          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|972963095|ref|XP_015198937.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Lepisosteus oculatus])

HSP 1 Score: 801.586 bits (2069), Expect = 0.000e+0
Identity = 510/1493 (34.16%), Postives = 783/1493 (52.44%), Query Frame = 0

Query: 2006 LCNICVINLMNLSKDKHTXTVNEK-----XAIYITQILTE--IVVSILYDKSLEIYTLLKNLSSENSPQHTNSTYLEDKRNIVPTKIIDALSSVILSQRLSDLDSMTSCKTWAAKMIVKCIGFHLSNEPIKGFKNHSDILGALPEDIYSYQLSGHAHKIVSMTSYND--ILATGSCDGTIRLWK--DNEYKRILIIDFDDESTLEISVMSD---------QILTWSPSGKYLVLSVGNIIYIWDMEDSEENCNQIPQLSPIIYLCWSPNSEI--------EILLAARQNGTSISITTLSWSSNSDYLAIGY---------ENGEILVLQIDQ-------------------------RKESIHLYGSQIVSYLLFH---------KSDPYILASGSSKNPGGIVNIWSILSQSVIRTLI-GDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE-MDNVIIASKCRAELFEH-LPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP----------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSS-PTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSS--SIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSL----HYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRN 3346
            L  +C  NL++ +   +  TV++         ++  +  E  +V S  +  +  +  LL +  ++  P +  +    +KR   P ++ +AL++  LS RLS     +  + WAA+ +V+ +  H  +   +  +  +D+ G L +  + ++L  H  ++++    N   +LAT   DGT+R+W    N+Y       F+   +     +S+           L+WS SGKYL  ++  ++ IW +   +      P    +  L W     +        E+LL  R +G S+ +  ++ +S    L + +         +NG + V  + Q                         R     L G+  V  L  H           D   L SG     GG++NIWS+   SV++T++ G G I  + WI    +A     +++V +++  ++ +    + + CR  L +  +     AP  RAF+++    + EQY YEK  +  G QL++S ++Q L+ LA  L L+ +LC+ P                 S W WL  F+ ++     L +         +  L    E +  + + +  W    D  +M W    P DW  GGK  V+L G G++GQLA+ G + + P  A SF  +  VICG   TF +Q NG++ ACGEGSYG+LG GNSDD   LT        VVT L++      H++       VFSWGDG+ GKLGHGN++R RRP+ I +L G     ++ G  HSAV+T  GK++TFGNGD GRLG GN   KK PE++++L GY V  VACGL HT                                 K++      + K+  G  FS+ LT  G V+  G  +  G+    +  N   P+++P L  +   DIAVG +H L L+ +G V+ WG N  GQLG       +EP ++  L GK I QISAG+ HSAAWT+PP+P     SS P +    +G P S+P ++ S L   S      RL+LL+  S+ + + WRL  +  ++             +    +R  L+P V TLP+++++  TM      +NYGPQ+TV+R+   G     IF QIA QV  ++  +LRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   +++LL P+PN  +++G N+D++L N        +  F FLG+L GVA+RT+KP+ ++LAP+VWK +  +    +DLE+VDL Y ++L    H   +    +N  D ++P D F+G S  G+ VP+ PG   I L++ N+ EYV  A+  R  EM  QV AVR+G+S + P P+L LL ++ +E +VCG     + +LKK+ RYR++D  ++++ W W+ LEEF+N+ ++LF+ FVSGRSRLP +  D+SQRFQI++V+RP + LPT+QTCFFQLRLPPY+S+ +M  RL YAI +CR+IDMD+YML+RN
Sbjct: 3276 LVQLCTQNLISAATGMNLTTVDDPIQRKFLPSFLRGVAEENKLVTSPNFVVTQALVALLADKGAKLRPNYDKTDV--EKRG--PLELANALAACCLSSRLS-----SQHRQWAAQQLVRTLAAHDRDNQSRP-QTFADMAGDLRKCSF-FKLEAHQSRVITCGWCNKKGLLATSGNDGTVRVWNVTKNQYTLQQTCVFNKSESTSEESLSNLGSPSDPSLSPLSWSVSGKYLAAAMEKMVNIWQVNGGKGLLAMQPHW--VSALAWPEEGPVSVWAGDTLEVLLVGRMDG-SLGLIEVADASTMQRLELEHCYRKDGKCCQNGLVCVWTVPQDTCSHSELSSGGSDEWWEMETKTKMRDCYRRLDGAVCVFQLKGHITPVRSVAFSPDGLALVSGGV---GGLMNIWSLRDGSVLQTVVAGSGAIQNIAWIPDVGVAACSNRSKDVLVVSCNSDFIGGHHVLATCRTALKKQGVTGLNMAPCMRAFLERLPVMLQEQYTYEKPHVVCGDQLVHSPYMQCLASLAVGLQLDPLLCNPPVPPHLQHCPPDLGSWNPSEWAWLDCFSTTVQAAEALARGAHFPGSFTVPDLEPVPEEELALLMDNSKWISGMDEQIMSWATTRPEDWHLGGKCDVFLWGAGRHGQLAEAGRNILVPTSAPSFSQAQHVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHSMALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTADGKLYTFGNGDYGRLGLGNTSNKKLPERVTALEGYQVGQVACGLNHTLAVSADGMMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVSCGTQFSVALTKDGHVYTFGQDRLIGLPEGRAR-NHNRPQQVPVLSGLFIEDIAVGAEHTLVLTSTGDVYAWGSNSEGQLGLGHTNHVREPSLVTALQGKRIHQISAGRCHSAAWTAPPVPPRAPGSSVPLQ----LGLPVSVPPQY-SALKEVSMETVRARLRLLYHFSDLMYSSWRLLNLSPNNQSCTSHYNAGTWGIVQGLLRPLLAPRVYTLPMVRSIGKTMVQG---KNYGPQITVKRISTRGRKCKPIFVQIARQVVKLNASDLRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELETGIVDLLIPSPNATAEVGYNRDRFLFNPSACLDEHLMQFKFLGILMGVAIRTKKPLDLHLAPMVWKQLCCVPLILEDLEEVDLLYVQTLNSILHIEDSGITEENFHD-MIPLDSFVGQSADGKMVPIIPGGNSIPLTFSNRKEYVDRAIEYRLHEMDRQVAAVREGMSWIIPVPLLSLLTAKQLEQMVCGMPEISVEVLKKVVRYREVDEQHQLVQWFWQTLEEFSNEERVLFMRFVSGRSRLPANTADISQRFQIMKVDRPYDSLPTSQTCFFQLRLPPYSSQPVMAERLRYAINNCRSIDMDNYMLSRN 4741          

HSP 2 Score: 326.25 bits (835), Expect = 2.789e-85
Identity = 233/756 (30.82%), Postives = 370/756 (48.94%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHPLS--STVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQIPQASTS------CYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINPV------------IFNKAVQLAQG--------------RTYDSHTIYLKEAAMYLTEQIVHFTSRNKQ--------------------DIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNH-------------FSNVHFPS--PIIDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWT-MRKGKGCFHW--PYSNEPDAKTFSQVSDILYNFGLSDFNAKLN--SHFDEDEQQKFLLHIFTLLISYFE-TISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLI 742
             +AL+ +G++TGLSLLF+LL+Q+W  P     L+  + VI TA +++ S PPLSL     +PP+G   L  V TFL     +P +           ELLL LA QRG    +L WI  AL  +    ++  N V              N  +Q+ +               RT D     L EAA+ L E++    S   +                    ++Y WG +            +  PK+     D + IE G  CT+VI ++G V +C K ++                + F     I   S S+ ++   LA T + +V+SWG+G +G+LGHG+     +PK I    P     ++C+SAG+ H+AA++ DG LYTWGEG  GRLGH D     +PTLVK++ NVG VSCG+ HT+ +SKDG  VWSFG G+NG+LG GDT  +Y PK+     G+ ++KV A ++ SLAL+ +G ++ WG            ++  PK +  +++  + ++S G  H + +  +  VY+WG N+ G+CG G ++  +  PK+   LD   I  I AG + S AWT + + +    W  PY  + +  TFS +   L  +     N+++         E   FL     LL ++    ++  + +     +   L +++F+ +D  ++  EI   VI+ + VGA  L P L++R+   + LL     +  +LSKGQ+ +L + L SL +   ++ L+
Sbjct:  154 TDALIEMGVRTGLSLLFALLRQSWMMPPPGPGLNLCNDVIHTAIDVISSLPPLSLANESKIPPMGLDCLAQVTTFLK-GVTMPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMALAASAVVSTMEKNKVPLSQEGLIGYDCFMNILMQMRRSLGSSADRSQWREPTRTPDG-LCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGEAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFADAQTIEAGQYCTFVISSDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRAIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQG--PLQGKVVVCVSAGYRHSAAVSEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVTEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLVEELATTRVVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVIGLDGVAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSHLRSFLERY-CDGINSEVPPLPFPSSREHHSFLKLCLKLLSNHLALALAGGVATSILGRQARPLRNLLFRLMDS-SVPDEIQEAVIETLSVGATMLLPPLRERMELLHSLLPQGPDRWESLSKGQRMQLDIILTSLQDHTHVASLL 903          

HSP 3 Score: 173.711 bits (439), Expect = 4.616e-39
Identity = 116/368 (31.52%), Postives = 181/368 (49.18%), Query Frame = 0

Query:  260 NKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPI----------IDASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-----PWNTSS--TVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWT 582
             K D++ WG   H            P      +  + +  G +CT+VI  NG V    +  +         ++H  + I          +  S   D  S  +ALTE  +V+SWG+G +G+LGHG+      P++I +L    ++ +S G  H+A +T DG LYT+G G  GRLG  +     +P  V  L  + VG V+CG  HTL VS DGM VW+FG G+ G+LG G++    +P+ ++   G+ +KKV    +FS+AL+  G ++ +G+      P   +     P+ +  +S   I +++ G  H +V+     VY+WG N+ G+ GLG T+  V+ P     L    I  I AG   S AWT
Sbjct: 3886 GKCDVFLWGAGRHGQLAEAGRNILVPTSAPSFSQAQHVICGQNCTFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQLVTSCGSDGHS--MALTESGEVFSWGDGDYGKLGHGNSDRQRRPRQIEALQGEEVVQMSCGFKHSAVVTADGKLYTFGNGDYGRLGLGNTSNKKLPERVTALEGYQVGQVACGLNHTLAVSADGMMVWAFGDGDYGKLGLGNSTAKSSPQKVDVLCGIGIKKVSCGTQFSVALTKDGHVYTFGQDRLIGLPEGRARNHNRPQQVPVLSGLFIEDIAVGAEHTLVLTSTGDVYAWGSNSEGQLGLGHTN-HVREPSLVTALQGKRIHQISAGRCHSAAWT 4250          

HSP 4 Score: 172.555 bits (436), Expect = 9.413e-39
Identity = 128/369 (34.69%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 2546 VYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPV-------VTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESVT---------KICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLEN--MVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG--NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            VY+ G+  + QL +     I  PK A SF  + ++  G   TF +  +GS+ ACG+GSYG+LGLG+S++  +L  +       +  + S      H L       VFSWGDG+ GKLGHGN+   + PK+I   L G     V+ G  HSA +++ G+++T+G GD GRLGHG+  ++  P  +  +    V  V+CG  HT                          N  ++  VT         K+C G   SL LTS G V+  G     G    SS      PK + +L    +VDI++G  H LALS    V+ WG N +GQ G  N +G   K   +I  L G  I QISAG +HS AWT+ P
Sbjct:  374 VYVWGSNSSHQLVEGTQEKILQPKLAPSFADAQTIEAGQYCTFVISSDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRAIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVSEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVTEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLG--CGSSEATALRPKLVEELATTRVVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVIG-LDGVAIQQISAGTSHSLAWTALP 737          

HSP 5 Score: 114.005 bits (284), Expect = 5.559e-21
Identity = 164/754 (21.75%), Postives = 316/754 (41.91%), Query Frame = 0

Query:  981 LESILTSFNLDYIQSVQTLLL-DLEGVQISNNNIKILHAIYYRMFSKLAFECGTSQDDFQPKICSLLDHLLQFINIEE-SDELNIENFEDFLTLKICCLSIEVYCSERVDIVLSEDFLKALCNILANNISNNLRSCVLRLIFQSILLARNVSKKLLN-EPCCYKISLIIQDQLEVNFGHDEILSDIVNFICVLTSSINESVVPSFLSKFKFGFWVNNLL-----ECLTGCPSI----LELSIVRCLGKILSHFEIGRNREH---IVRKLFSHIYNSYWNSKSSDLDFTNIQRTSYNME--------------------FNLDNSYHCILQDTKTVVSSGELFGYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMW------------RSIIDSCSHN--IVLKSYELVKDLLKXFSSLISQSEKLRGSIDNVLE-----------AIYPLLALLSDVDSGLCLGSYVRYKEFKR-GIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKI------------FMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQA--VENMTDSLVSSIIDEVTGKSESGNKVN 1653
            LE  L + N  +I++ Q LLL  +  + +    + +  AI   + + L+  CGT     QP        LLQ   + + S  L + +      L+I  ++   Y  +     LS   +++L ++L + + N L    + L+        N S++ ++ E   ++  L  Q   E++ G         +F+  L   ++   + S ++  K   W   LL     +C +G P +      L  +  L  +L   E     +    +V +LFS + +  W +  ++   T IQ      E                    F+ + +  C++++ + +       GY +A+  I+ G   WK  I+K+++G +   +GV+   +   N+R+  DMWLY + SG L H  G  +  L SF++GD + C+L+ E  ++SF KNG    LAF D+  + ELYP V F S+    K+ I ++ +++ +   L   +P    ++  +A+ T ++IR L+  ++ +W            R I   C      + KS  +     +     + + +K R     + E            ++P+LA++   D+GL +G    +K+  R   ++G+    S + K++W++    +  +I         D+P++ +            F    +S L+D+  L     +   LS     K     ++ K +P   ++    +  TD  +S  +DEV   + S    N
Sbjct: 1739 LERALQA-NKHHIEAQQRLLLVTVFALSVRYQPVDVSLAISSGLLNVLSQLCGTDTMLGQPL------QLLQKPGVSQLSTALKVASTR---LLQILAITTGTYADK-----LSPKVVQSLLDLLCSQLKNLLSQAGVSLLPPFREEEENKSEESVDPEKKDFRALLRAQHTAELHLG---------DFLVFLRRVVSSKAIQSKMASPK---WTEVLLNIAAQKCCSGVPLVGNLRTRLLALHVLEAVLPACEASVEDDQMTQVVERLFSLLSDCMWEAPIAEAKHT-IQVKEKEQELKLQGEAEEEDENLPIQEVSFDPEKAQCCVVENGQGLTHGSGGKGYGLASTGITSGCYQWKFYIVKENRGNEGTCVGVSRWPVHDFNHRTTSDMWLYRAYSGNLYHN-GEQTLTLSSFTQGDYVTCVLDMEARTISFGKNGEEPKLAFEDV-DATELYPCVMFYSSNPGEKVKICDM-QMRGTPRDLLPGDPICSPVATVLAEATTQLIRILH--RTDLWTHCINGNMMERLRGIKACCREGGQRLRKSRSVQSREEREAREELREEDKGRPGRHGLAELSEAQLKALCTEVWPVLAVIGGADAGLRVGGRCVHKQTGRHATLLGVVKEGSTSAKVQWDE----AEITISFPTFWSPSDTPLYNLEPCEPLPFDVSRFRGLTASLLLDLTYLTGIHEDSGKLSTRRHEKKHRHESEEKGDPEQKTEGEHPKEATDLRISHSLDEVKAHAASCKSEN 2455          

HSP 6 Score: 89.3521 bits (220), Expect = 1.410e-13
Identity = 80/278 (28.78%), Postives = 125/278 (44.96%), Query Frame = 0

Query: 2543 KSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVIC---GLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLA 2799
            +  V+  G+G  G+L    SS+   PK          V+C   G + + A+  +G LY  GEG +G+LG G+S+     T V               T+ LS +   V+S+G G++GKLGHG+T R  +PK+  +L G F   V  G+  S  LT  G+++ +G G    LG G+        + ++LR   V  +A               V  I +G +  L L+   +V+  GN+        +S   I  PKK+  L+ +    I+ G  H LA
Sbjct:  476 EGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVSEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVTEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCG--ACLGCGS-------SEATALRPKLVEELAT------------TRVVDISIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVIGLDGVAIQQISAGTSHSLA 732          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|992201025|ref|XP_015460465.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Astyanax mexicanus])

HSP 1 Score: 795.038 bits (2052), Expect = 0.000e+0
Identity = 493/1309 (37.66%), Postives = 717/1309 (54.77%), Query Frame = 0

Query: 2154 AHKIVSMTSYNDILATGSCDGTIRLWKDNEYKRILIIDFDDESTLEISVMSDQI--LTWSPSGKYLVLSVGN--IIYIWDMEDSE-ENCNQIPQLSPIIYLCWSP----NSEIEILLAAR------------QNGTSISITT-LSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLI-GDGGITCLHWINSKTLAVGFCNTENVTLMNL-PNEMDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP--------------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLFFDEIKKDVN-LKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSL----HYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
            A + +S  S +   A G  DG I L   +         FD E  + ++   + +  L W P+G +L+LSVG   ++ IW          + +     +    W P     ++  +L+A              Q GTS+++   L  S++ D  A+        V ++     S H+     +  L F  S   +++ G     GG++NIWS+   SV++T++ G G + C  W+    +A     +++V L+   P+ +    + + CR  L  + +     AP    F+++    + EQY YE+  +  G QL++S FLQ+L+ LA  L+L+ +L   P                     S W WLS+F+ ++       +  A  +   +  L    + +    L +  W+   D  LM W    P DWQ GGKS V+L GNG++GQLAD G++   P  A S   +  V+CG   TF +Q NG++ A GEGSYG+LG GNSDD ++ T        VVT L++      H+L       VFSWGDG+ GKLGHGN+ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+GD GRLG  +   K  PE++++L GY +  V+CG+ HT                                 K+     + + K+  G  FS+ L   G V+  G  +  G+  +S   N   P+ +P LE +   DIAVG +H+LALS +G V+ WGCN   QLG       KEP ++  L GK I QISAG+ HSAAWT+P + +  + S+  +    +G P S+P ++ + L   S     TRL++L+  S+ +   WRL  +   +     +      A+    +R  LSP V TLPL++++  TMT     + YGPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   V++LL  TPN  +D+GSN D++LLN    S   M  F FLG+L  VA+RT+KP+ ++LAP VWK +  I     DLE+VDL   RSL    H   +S N +N    ++P D F+  S  G+ VP+ PG   + L++ N++EYV+ AL  R  EM  QV AVR+G+S++ P P+L LL ++ +E LVCG     + +LKK+ RYRD+  S+++IVWLW+ LEEF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFFQLRLPPYTS+ ++  RL Y+I +C +IDMD+YML+RN E  P  G D
Sbjct: 3311 APQCLSWYSTDRPFAVGYPDGKILLASTD--------SFDTEQPILLTAFHESVSALRWDPTG-HLLLSVGRSEVVKIWGRAGGTWAILHSLFHTGTVNAAEWCPLPGRGTDPRLLMAVGCQNGLLYVWTVPQGGTSVTVPNFLVASTSQDRSAVFKRGNAKCVFRL-----SGHITA---IKTLSFCPSGQALISGGI----GGLLNIWSLKDGSVLQTVVTGLGSVVCTTWVPQVGVATCSGRSKDVLLIRCSPDWISQNHVLASCRTVLRSQGILGLNRAPCMAVFLERLPVLLQEQYNYERSHVMAGDQLVHSAFLQSLASLAVGLSLDQLLSRQPVPPHHIGSTTGPEPATAPSAQSEWAWLSTFSITVKSAEAFARGTAFPEAFTMPELQQPGVTESARALDNSKWSFMMDEQLMLWATSRPEDWQQGGKSEVFLWGNGRHGQLADAGTNVSVPTLASSLSQTQQVVCGQNCTFLVQANGTVLAVGEGSYGRLGQGNSDDLYTPTIISALQGYVVTQLVTSYGSDGHSLALTETGEVFSWGDGDYGKLGHGNSERQRRPKQIEALQGEEVMQLACGFKHSAVVTADGKLFTFGSGDSGRLGQRSTSNKMVPERVTALEGYHIGQVSCGINHTLALSSDGMTLWAFGDGDYGKLGTGPCTVKCYPQKVETLCNKGIKKVGCGTHFSVVLAKDGHVYTFGQERLIGLP-DSMLKNHNRPQIVPTLEGIFIEDIAVGCEHILALSSTGDVYAWGCNCEAQLGLGHSSPVKEPTLVTALQGKNIRQISAGRCHSAAWTTPSLSSRNSGSAGLQ----LGLPQSIPPQY-NALKDCSPEALSTRLRVLYHFSDLMYKSWRLLNLDPRNQVPASRYSSGTAAIVQGQLRGLLSPKVNTLPLVRSIGRTMTQG---KTYGPQITVKRISTRGRSSKPIFVQIAKQVVNLNPTELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGVVDLLIHTPNSSADVGSNTDRFLLNPAAVSDEHMVQFRFLGILMAVAVRTKKPLDLHLAPWVWKQLCCIPLGGADLEEVDLLTYRSLQGILHLDDSSINEENF-TVMIPLDSFVAHSADGKLVPVVPGGHNLPLTFTNRNEYVERALEYRLHEMDRQVAAVREGMSSIIPVPLLSLLTARQLEQLVCGLPEVSVEMLKKVVRYRDITESHQLIVWLWQSLEEFTNEERVLFLRFVSGRSRLPSNPADITQKFQIIKVDRPVNGLPTAQTCFFQLRLPPYTSQAVLAERLRYSIHNCPSIDMDNYMLSRNTE--PVDGSD 4586          

HSP 2 Score: 301.597 bits (771), Expect = 8.578e-78
Identity = 230/759 (30.30%), Postives = 360/759 (47.43%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPD---EQHP----LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQ----IPQASTSCYELLLSLA-IQRGRCSDVLHWINTALTVAIQNPSLTINP-------VIFNKAVQ-LAQGRTYDSHT----------------IYLKEAAMYLTEQIVHFTSR----------------NKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNFGLSDFNAKLNSHF-DEDEQQKFLLHIFTLLISYFETI-SSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLLKMN-----NLSKGQQYKLKMDLESLNEPGIISGLICESEEG 748
             + L+ +G++TGLSLLF+LL+Q+W       E+ P    L + V+ TA+++L + PPLSL     +P +G   L  V+ FL  +A       QA       LL    +QRG    +L W+  AL  +  + S +          V +    Q L Q R Y                      L   A+ L E+I    S                 +   +Y WG +            +  PK+    +D + IE G  CT+ +  +G V +C K ++        +N   P  ++          S S+ ++   LA+T + +V+SWG+G +G+LGHG+     +PK I    P     ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     VPTLVK++  VG  +CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ WG           T+S  P++I    ++ I ++S G  H + +     VY+WG N  G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ +   L  +  S       + F  + E  +F+L    LL  +     +    +    A+   L +++F+ ID  N+   +   V+  + +GA  L P L++R    + LL        +LSKGQ+ +L M L SL E   ++ L+  S  G
Sbjct:  164 TDVLIEMGVRTGLSLLFALLRQSWQRGRLSLEEDPPDVALCNEVLGTASSVLAALPPLSLANENKIPSVGLDCLAQVSDFLKKTAVSGSWADQAGRRLALELLLGLAVQRGSLRFLLEWVEVALATSASSSSSSSPTSSSEPSGVGYELISQILQQMRQYSGFRGDYVNTQVLNKDADGLCSLSHVALCLFEEICALASNCLCSCSVGSTSPGSEGDAVKVYVWGSNSSHQLAEGTLEKILQPKLAQGFSDAQMIEAGQYCTFSVSADGSVRACGKGSYGRLGLGDSNNQSVPKKLVLDPPRTMRKVSSSKGSDGHTLAVTAEGEVFSWGDGDYGKLGHGNSATQKYPKIIQG--PLLGKVVVCVSAGYRHSAAVTNDGELYTWGEGDFGRLGHSDSHSRNVPTLVKDISGVGQAACGSSHTIAVAQDGRIVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGYIIRKVCAGSQCSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFIEELGVTKIIDISCGDSHCLALSHENEVYAWGNNAMGQCGQGHTSTPITKPKKVTGLEGVSIQQITAGTSHSLAWTAIPTDRQLVAWHRPFCVDLEESTFTYLRSFLERYCDSISGDTPPAPFCSKREHHQFVLLCMKLLSIHLSLAHAGGTGATVLGAQGRPLRNLLFRLIDS-NVPESLQQAVMNTLSIGASLLLPPLRERTELLHSLLPQGPESWESLSKGQRLQLDMVLNSLQEQSHVASLLGYSSPG 919          

HSP 3 Score: 169.859 bits (429), Expect = 6.266e-38
Identity = 128/373 (34.32%), Postives = 175/373 (46.92%), Query Frame = 0

Query: 2541 GGKSSVYLVGNGQNGQLADLGSSSI-PPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDY-----FSLTPVVTLLL--------SHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            G    VY+ G+  + QLA+     I  PK A  F  +  +  G   TF++  +GS+ ACG+GSYG+LGLG+S++        L P  T+           H L       VFSWGDG+ GKLGHGN+   + PKII   L G     V+ G  HSA +T+ G+++T+G GD GRLGH +  ++  P  +  + G  V   ACG  HT                          N  +R  V          K+C G   SL LTS G VF  G    S +   SS      P+ I +L    ++DI+ G  H LALS    V+ WG N +GQ G   +     +P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  379 GDAVKVYVWGSNSSHQLAEGTLEKILQPKLAQGFSDAQMIEAGQYCTFSVSADGSVRACGKGSYGRLGLGDSNNQSVPKKLVLDPPRTMRKVSSSKGSDGHTLAVTAEGEVFSWGDGDYGKLGHGNSATQKYPKIIQGPLLGKVVVCVSAGYRHSAAVTNDGELYTWGEGDFGRLGHSDSHSRNVPTLVKDISG--VGQAACGSSHTIAVAQDGRIVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGYIIRKVCAGSQCSLALTSAGQVFAWGCG--SCLGCGSSETTSLRPRFIEELGVTKIIDISCGDSHCLALSHENEVYAWGNNAMGQCGQGHTSTPITKPKKVTGLEGVSIQQITAGTSHSLAWTAIP 747          

HSP 4 Score: 164.081 bits (414), Expect = 3.745e-36
Identity = 108/379 (28.50%), Postives = 182/379 (48.02%), Query Frame = 0

Query:  247 MYLTEQIVHFTSRNKQDIYTWGCDLHS-----------PKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFSNV------HFPSPIIDASL----------SQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWT 582
            ++ T +   +    K +++ WG   H            P +   ++  +++  G +CT+++  NG V    +  +  +         +P I ++L          S  ++   LALTE  +V+SWG+G +G+LGHG+ +    PK+I +L    +M ++ G  H+A +T DG L+T+G G  GRLG +      VP  V  L  +++G VSCG  HTL +S DGM +W+FG G+ G+LG G       P+ +       +KKV     FS+ L+  G ++ +G+            +   P+ + ++    I +++ G  H++ +     VY+WG N   + GLG +S  VK P     L   +I  I AG   S AWT
Sbjct: 3711 LWATSRPEDWQQGGKSEVFLWGNGRHGQLADAGTNVSVPTLASSLSQTQQVVCGQNCTFLVQANGTVLAVGEGSYGRLGQGNSDDLYTPTIISALQGYVVTQLVTSYGSDGHSLALTETGEVFSWGDGDYGKLGHGNSERQRRPKQIEALQGEEVMQLACGFKHSAVVTADGKLFTFGSGDSGRLGQRSTSNKMVPERVTALEGYHIGQVSCGINHTLALSSDGMTLWAFGDGDYGKLGTGPCTVKCYPQKVETLCNKGIKKVGCGTHFSVVLAKDGHVYTFGQERLIGLPDSMLKNHNRPQIVPTLEGIFIEDIAVGCEHILALSSTGDVYAWGCNCEAQLGLGHSS-PVKEPTLVTALQGKNIRQISAGRCHSAAWT 4088          

HSP 5 Score: 98.9821 bits (245), Expect = 1.974e-16
Identity = 86/302 (28.48%), Postives = 145/302 (48.01%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKXFSSL---ISQSEKLRGSIDN----------------------------VLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWED 1551
            GY +A   I+ G   WK  I K+++G +   IGV+   I   N+R+  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  +  + ELYP V F S+    K+++ +L +++   S L   +P        + + TV+++R L++  S  W   ++   H  + +  E +  ++K  + L    S  E++ G + +                            +   ++P+LAL+  VDSGL  G    +K   +R  ++G+    S   K++WE+
Sbjct: 2018 GYGLATTAITSGCFIWKFYITKENRGNEGTCIGVSRWPIRDYNHRTTTDMWLYRAYSGSLYHG-GELGRALPSFTQGDTITCILDMEARTISFAKNNREPKLAFEGV-DATELYPCVLFYSSNPGEKVALCDL-QMRGMPSDLLPGDPLCSPQVTVLLEATVQLLRRLHH--SDDWAPHVN--LH--ISRRLEFIGPVMKEGTHLRHGQSSKEQVEGGMTDKERSPGENWRSPDLFSGPSGLSEVQLSVLCAEVWPVLALIGGVDSGLRAGGICLHKPSGRRATLLGVLKEGSPLAKLQWEE 2310          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|768919364|ref|XP_011602889.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 isoform X5 [Takifugu rubripes])

HSP 1 Score: 790.415 bits (2040), Expect = 0.000e+0
Identity = 487/1266 (38.47%), Postives = 699/1266 (55.21%), Query Frame = 0

Query: 2192 FDDESTLEISVMSDQI--LTWSPSGKYLV-LSVGNIIYI-------WDMEDS--EENCNQIPQLSPIIYLCWSPNSEIEILLAAR----------QNGTSISITTLSWS--SNSDYLAIGYENGEILVLQIDQRKESIHLYGS-QIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP-------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
            +D+E  + IS+  D +  L W P+G  L+ L    I+ I       W    S    +   I +  P+  L  +P+  + + +  +          Q GT++S+  +  S  S      +  E+ E + +          L+G    V  L F  S   +++ G     GG++NIWS+   SV++T+ G G +   +WI +  +AV F  +++V L+    E    N ++AS CR  L  +++     AP    F+++    + EQ+ YE+  ++ G QL++S FLQ+L+ LA  L++   LC LP              S W WL+S+  ++     +       +  L+      D+ +    L +  W+ + D  LM W  V P DWQ GGKS VYL GNG+ GQLA  G++   P  A S   +  V+CG   +F +Q NG + A GEG YG+LG GNSDD +  T        VVT L++      H++       VFSWGDG+ GKLGHGN ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+G+ GRLG  +   K  PE++++L GY V  V+CGL HT                                 K+     + + K+C G  FS+ +   G V+  G  +  G+  +S   N  +P+ +P LE +   DIAVG +HVLALS +G V+ WGCN  GQLG       KEP +I  L GK I QISAG+ HS+AWT+P   T+ N+  P   +  +G P S+P ++ + L   S      RL++L+  S+ +   WRL  +         +      A+    +R  LSP V TLPL++ +  TMT     + +GPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL    ++LL  TPNG +D+GSN D++LLN    S+  M  F FLG+L  VA+RTRKP+ ++LAP VWK +  I     D+E+VDL   R+L  +    N    ED+   ++P D FM  S  GR VP+ PG + I L++ N+ +YV+  L+ R  EM  QV AVR+G+S + P P+L LL  Q +E LVCG     + +LKK+ RYRD+  S+++IVW W+ L+EF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFF LRLPPYTS+ I+  RL Y+I +C +IDMD+YMLT N E   + G D
Sbjct: 3512 YDNEQPIVISIFQDSVSSLKWDPTGHLLLCLGKSEIVKILGCYKGTWVTLHSLIHSSTVNISEWCPLAGL--APDPRLMMAVGCQNGSVYVWTLPQGGTAVSVPNIKSSPPSQDKCTDLKQESAECVFV----------LHGHITAVRSLSFCPSGLALVSGGI----GGLLNIWSLQDGSVLQTVTGLGSVLSTNWIPNMGVAVCFGRSKDVLLICCTPEWISQNHLLAS-CRMVLRSQNILGLNRAPCLVVFLERLPLLLQEQHSYERSHVAAGEQLVHSAFLQSLASLAVGLSIEKHLCHLPRPPHHRSSESDSCPSEWSWLASYATTVQTAEAIASGTVFPESFLMSEFQELDDAESAKALDNSKWSYKMDEQLMSWANVRPEDWQEGGKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPTIISAFQGYVVTQLVTSCGSDGHSMALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLP-DSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCNPVKEPTLITALQGKNIKQISAGRCHSSAWTTPS--TSKNSGGPGNLQ--LGLPQSVPPQY-NTLKDCSPRVLSMRLRVLYNFSDLMYKSWRLLNLDPQSPLSTSRYSSGTAAIIRGDLRGLLSPKVNTLPLVRCIGRTMTQG---KTHGPQITVKRISTRGRSSKPIFVQIAKQVVSLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGAVDLLIHTPNGFADVGSNTDRFLLNPAAFSEDHMIQFRFLGILMAVAIRTRKPLDLHLAPWVWKQLCSIPLGGADIEEVDLLTYRTLQGILHLENSGITEDNFHVMIPLDSFMVHSADGRLVPVVPGGQNIALTFANRTDYVERGLDYRLHEMDSQVAAVREGMSTIIPVPLLSLLTPQQLEQLVCGLPEVSVEMLKKLVRYRDVTESHQLIVWFWQSLQEFTNEERVLFLRFVSGRSRLPSNPADITQKFQIIKVDRPINGLPTAQTCFFLLRLPPYTSQAILSERLRYSIHNCPSIDMDNYMLTHNTEPADSSGTD 4751          

HSP 2 Score: 300.056 bits (767), Expect = 2.682e-77
Identity = 242/764 (31.68%), Postives = 371/764 (48.56%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHP---LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQI-------PQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAI--------------QNPSLTINPVIFNKAVQLAQ--------GRTYDSHTI--------YLKEAAMYLTEQIVHF----------------TSRNKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDIL--YNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGK 749
             + L+ +G++TGLSLLFSLLQQNW      +P   L + V++TA ++L S PPLSL     +P +G   L  VA FL  ++ I       P       ELLL LA+QRG    +L W+  AL  ++              Q  +     VI    +Q+ Q        G + ++  +         L +A + L E+I +                 T  +   +Y WG +            +  PK+    +D + IE G  CT+ + T+G V +C K ++        +N   P  ++          S S+ ++   LA+T D +V+SWG+G +G+LGHG+     +PK I    P     ++  SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++  VG V+CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ WG           T+S  P+++   SI+ I ++S G  H + +     VY+WG NT G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ + + L  Y  GL +           D QQ  LL +  L +      +    +     +   L +++F+ ID  N+   I   V+  + +GA  L P L++R      LL      +N LSKGQ+ +L M L SL +   I+ L+  S  G+
Sbjct:  162 TDVLIEMGVRTGLSLLFSLLQQNWRYAASAYPESVLCNDVLATACSVLASLPPLSLANENKIPSVGLECLAQVAEFLKRTSVISGAGAADPTGRRLALELLLGLAMQRGSLKFLLEWVEVALAASMSSSNSMPSSSAMTSQKSAGVGFDVIQQTLLQMRQYSFLQGFSGDSINTQVLKKDADGLCQLSQAVLCLFEEICNLASYCLCSSSTDAASPGTEGDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQSLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQG--PLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISGVGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVPTDRQLVAWHRPFCVDLEESTFTYLCNFLKCYCDGLENDTPPTPFLSKRDHQQFILLCMKLLSVHLSLAHAGGTGALVLGTQGRPLRNLLFRLIDS-NMPDSIQKAVLNTLSIGASLLLPPLRERTELLLSLLPRGPQSLNVLSKGQRLQLDMVLSSLQDQSYIASLLGYSSLGE 922          

HSP 3 Score: 173.326 bits (438), Expect = 5.368e-39
Identity = 124/416 (29.81%), Postives = 198/416 (47.60%), Query Frame = 0

Query:  260 NKQDIYTWG-----------CDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPIIDA-----------SLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWTM------RKGKGCFH--WPYSNEPDAKTFSQVSD--------ILYNF 613
             K D+Y WG            +L  P +   ++  +++  G +C+++I +NG V+   +  +         +++ P+ II A           S   D  S  +ALTE  +V+SWG+G  G+LGHG+ +    PK+I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG +      +P  V  L  ++VG VSCG  HTL++S DGM VW+FG G+ G+LG G +   Y P+ +       MKKV    +FS+ ++  G ++ +G+            + + P+ + S+    I +++ G  H++ +     VY+WG N+ G+ GLG  +  VK P     L   +I  I AG   S AWT         G G      P S  P   T    S         +LYNF
Sbjct: 3887 GKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPT-IISAFQGYVVTQLVTSCGSDGHS--MALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLPDSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCN-PVKEPTLITALQGKNIKQISAGRCHSSAWTTPSTSKNSGGPGNLQLGLPQSVPPQYNTLKDCSPRVLSMRLRVLYNF 4298          

HSP 4 Score: 167.548 bits (423), Expect = 3.577e-37
Identity = 126/374 (33.69%), Postives = 173/374 (46.26%), Query Frame = 0

Query: 2541 GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            G   +VY+ G+  + QLA+     I  PK    F  + ++  G   TF++  +GS+ ACG+GSYG+LGLG+S++  SL   + L    N+                     VFSWGDGE GKLGHGN+   + PKII   L        + G  HSA +T  G+++T+G GD GRLGH + Q++  P  +  + G  V  VACG  HT                          N  +R  V          K+C G   SL LTS G VF  G    S +   SS      P+ +  L    ++DI+ G  H LALS    V+ WG N +GQ G   +      P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  381 GDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQ-SLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQGPLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISG--VGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCG--SCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVP 749          

HSP 5 Score: 104.76 bits (260), Expect = 3.406e-18
Identity = 123/524 (23.47%), Postives = 224/524 (42.75%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKX-----FSSLIS-------QSEKLRGSIDN-------------------VLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKI------------FMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSL 1761
            GY +    I+ G   WK SI K+++G +   IGV+   +   N+ +  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  + +S ELYP V F S+    K+S+ +L +++   + L   EP     S  + + T++++R L+  +   W  +I+      +    EL+  LLK       SS +S       Q E+ + ++D                    +   ++P+LA++  VD+GL  G   ++    ++ +++G+    S   K++WE+    +  S+  +      D+P+  +                 S+ L+D++ L+    E+  L+    +K +  +   +EN    S   +    S  +      +   +    V++ E       S  TLH       A +  +++    L          AL + +  + + +    + TP ++ S S     S  E+T        RNRQ  + + L + L
Sbjct: 2005 GYGLTTTAITSGCFIWKFSISKENRGNEGTCIGVSRWPVRDHNHHTTTDMWLYRAYSGNLYHG-GELIRTLPSFTQGDTITCILDMEAHTISFAKNDKEPKLAFEGVIAS-ELYPCVLFYSSNPGEKVSLHDL-QMRGMPNNLLPGEPLCSPRSTVLVESTIQLLRRLH--QCDQWAPLIN----QYIYSQLELIGPLLKEDDLSHSSSDVSPIHFEKEQREREKDTLDTDRGELYLHRRALSDAKLAALCSEVWPVLAVIGGVDTGLRAGGLCQHAPSGRKAVLLGVLKEGSSMAKLQWEE----TELSVSSMNSWSPSDTPITSLEPCSTSCFDVTPLGGLRSTVLLDLIYLMGLLEEQGWLATHQDHKIKCSQENMQENEIQRSNDEDGKEKSPETDSFSSSSSTRDPRKIVDHQESTCPTQPSQ-TLHSSKLDAFAFERRAVRVSYLLI--------GALKSLTHIFSSENPADLLQTPKHDPSNSSTTPLSSPEQTRE-----ERNRQWDERAELRSVL 2501          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|736250299|ref|XP_010787812.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Notothenia coriiceps])

HSP 1 Score: 790.415 bits (2040), Expect = 0.000e+0
Identity = 481/1253 (38.39%), Postives = 686/1253 (54.75%), Query Frame = 0

Query: 2192 FDDESTLEISVMSDQI--LTWSPSGKYLVLSVGN---IIYIWDMEDSEENCNQIPQLSPIIYLCWSPNS----EIEILLAA------------RQNGTSISITTLSWSSNSDYLAIGYENGEILVLQIDQRKESIHLYGSQIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNL-PNEMDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP-------------DSRWKWLSSFTQSMTFISLLNKRNA-KSDHELLKSLFFDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNE 3348
            +++E  + +SV  D I  L W P+G +L+L +G    +  +     S    + +   S +    W P +    +  +++AA             Q GT +S+  +  SS     + G E  E         K    L+G       L   S    L SG     GG++NIWS+   SV++T+ G G +    WI +  +A  F  +++V L+   P+ +    + + CR  L  +++     AP F  F+++    +  QY YEK  ++ G QL++S FLQ+L+ L   L+L   LC  P              S W WL+++  ++     +    A      L +    D  +    L +  W+   D  LM W    P DWQ GGKS VYL GNG+ GQLA +G++ + P  A S   +  V+CG   +F +Q NG++ A GEG YG+LG GNSDD +  T        VVT L++      H++       VFSWGDG+ GKLGHGN+ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+G+ GRLG  +   K  PE++++L GY V  V+CGL+HT                                 K+     + + K+C G  FS+ L   G V+  G  +  G+  +S   N   P+ +P LE +   DIAVG +HVLALS +G ++ WGCN  GQLG       KEP  I  L GK I QISAG+ HSAAWT+P   T+  NS  T     +G P S+P ++ + L   S      RL++L+  S+ +   WRL  +         +      A+    +R  LSP V TLPL++++  TMT     + YGPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL   V++LL  TPN  +D+GSN D++ LN    S+  +  F FLG+L  VA+RT+KP+ ++LAP VWK +  +     DLE+VDL   R+L  +    N    ED+   ++P D FM  S  GR VP+ PG + I L++ N+ EYV+ A++ R  EM  QV AVR+G+S + P P+L LL +Q +E LVCG     + +LKK+ RYRD+  S+++I W W+ LEEF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFF  RLPPYTS+ I+  RL Y+I +C +IDMD+YMLT N +
Sbjct: 3561 YENEQPVVLSVFQDGISSLKWDPTG-HLLLCLGRSEVVKILGRSGGSWVTLHSLTHSSTVNIAEWCPVAGRAPDPRLMVAAGCQNGSVHVWTLPQGGTCVSLPNILNSSQGQEKSTGSEKQE-------SAKSVFVLHGHITAVKSLSFCSSGLALVSGGI---GGLLNIWSLQDGSVLQTVTGLGSVVSTTWIPNLGVAACFGRSKDVLLICCTPDWISQNHVLASCRMVLRSQNVLGLNRAPCFAVFLERLPLLLQAQYNYEKTHVAAGDQLVHSAFLQSLASLTVGLSLEKHLCRYPCPPHHSSPDAHSCPSEWSWLATYATTVRSAEAIASGTAFPESFSLSEVQEVDATEITTALDNSKWSFRMDEQLMSWATTRPEDWQLGGKSEVYLWGNGRYGQLAGIGTNLMMPTLAPSLLQTQQVVCGQNCSFLVQSNGTVLAVGEGQYGRLGQGNSDDLYIPTIISAFQGYVVTQLVTSCGSDGHSMALTETGEVFSWGDGDFGKLGHGNSERQRRPKQIEALQGEEVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLKHTLVLSLDGMVVWAFGDGDYGKLGTGSSTAKYYPQKVEQLCNKGIKKVCCGTQFSVALACDGHVYTFGQERLIGLP-DSMLKNKSCPQVVPSLEGLFIEDIAVGCEHVLALSSTGDIYAWGCNSEGQLGLGHSNLVKEPTFITSLQGKNIRQISAGRCHSAAWTTPS--TSLKNSG-TSGSFQLGLPQSVPPQY-NTLKDCSPHVLSMRLRVLYHFSDLMYKSWRLLNLDTKKPVSTSRYSSGTTAIIRGELRGLLSPKVNTLPLVRSIGRTMTQG---KTYGPQITVKRISTRGRSSKPIFVQIAKQVVSLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGVVDLLIRTPNSFADVGSNTDRFFLNPAALSEDHIVQFRFLGILMAVAIRTKKPLDLHLAPWVWKQLCSMPLGGGDLEEVDLLTYRTLQGILHLENSGITEDNFHVMIPLDTFMAHSADGRLVPVVPGGQNISLTFSNRTEYVEKAMDYRLHEMDSQVAAVREGMSTIIPVPLLSLLTAQQLEQLVCGLPEVSVEMLKKLVRYRDITESHQLIGWFWQSLEEFTNEERVLFLRFVSGRSRLPSNPADIAQKFQIIKVDRPVNGLPTAQTCFFLFRLPPYTSQAILAERLRYSIHNCPSIDMDNYMLTHNTD 4794          

HSP 2 Score: 308.145 bits (788), Expect = 8.658e-80
Identity = 247/766 (32.25%), Postives = 378/766 (49.35%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHP---LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLH-------TSAQIPQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAIQNPSLTINP----------------VIFNKAVQLAQGRTYDSHTI-------------YLKEAAMYLTEQIVHFTS-------------RNKQD---IYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIW------GKTPWNTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDILYNF--GLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGKDAA 752
             + L+ +G++TGLSLLFSLLQQNW      HP   L + V++TA+++L S PPLSL     +P +G   L  VA FL        T    P       ELLL LA+QRG    +L W+  AL  ++ + S T++                 VI    +Q+ Q   +   ++              L +AA+ L E+I +  S              ++ D   +Y WG +            +  PK+    +D + IE G  CT+ +  +G V +C K ++        +N   P  ++          S S+ ++   LA+T + +V+SWG+G +G+LGHG+     +PK I    P     ++C+SAG+ H+AA+T DG LYTWGEG  GRLGH D     VPTLVK++  VG V+CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ W      G     T+S  P++I   SI+ I ++S G  H + +     VY+WG NT G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ + + L ++  G+ D N         D  Q  LL +  L I      +    +    A+   L +++F+ ID  N+   I   V+  + +GA  L P L++R      LL      +N LSKGQ+ +L M L SL +   ++ L+  S  G+  A
Sbjct:  162 TDVLIEMGVRTGLSLLFSLLQQNWRYAASVHPESVLCNDVLATASSVLTSLPPLSLANENKIPTVGLDCLAQVADFLKKTSVSSGTGGADPTGRRLALELLLGLAMQRGSLKFLLEWVEVALAASLPSSSSTLSSSSSSLSSQHSAGVGFDVIHQTLLQMKQYSGFRGDSVNTQVLKKDADGLCQLSQAALCLFEEICNLASYCLCSCSTDAAAPGSESDTVMVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQMIEAGQYCTFSVSADGSVKACGKGSYGRLGLGDSNNQSMPKKLVLEPHRNMKKVSSSKGSDGHTLAITAEGEVFSWGDGEYGKLGHGNSATQKYPKIIQG--PMFGKVVVCVSAGYRHSAAVTNDGELYTWGEGDFGRLGHSDSQSRNVPTLVKDISGVGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGSGSAETTSLRPRFIEDLSITKIIDISCGDSHCLALTHENEVYAWGNNTMGQCGQGHTSTPITKPKKVLGLEGVSIQQITAGTSHSLAWTAVPTDRQLVAWHRPFCVDLEESTFTYLRNFLESYCDGIGDDNPPPPFLSKRDHHQFILLCMKLLSIHLSLAHAGGTGAMVLGAQGRPLRNLLFRLIDT-NMPDSIQQAVLNTLSIGASLLLPPLRERTELLLSLLPQGPQSLNVLSKGQRLQLDMVLSSLQDQSHVASLLGYSNFGEVTA 924          

HSP 3 Score: 173.711 bits (439), Expect = 4.665e-39
Identity = 112/369 (30.35%), Postives = 188/369 (50.95%), Query Frame = 0

Query:  260 NKQDIYTWG-----------CDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPIIDA-----------SLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWT 582
             K ++Y WG            +L  P +   +   +++  G +C++++ +NG V    +  +         +++ P+ II A           S   D  S  +ALTE  +V+SWG+G  G+LGHG+ +    PK+I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG +      +P  V  L  ++VG VSCG +HTL++S DGM VW+FG G+ G+LG G +   Y P+ +       +KKV    +FS+AL+  G ++ +G+            + + P+ + S+    I +++ G  H++ +     +Y+WG N+ G+ GLG ++L VK P     L   +I  I AG   S AWT
Sbjct: 3937 GKSEVYLWGNGRYGQLAGIGTNLMMPTLAPSLLQTQQVVCGQNCSFLVQSNGTVLAVGEGQYGRLGQGNSDDLYIPT-IISAFQGYVVTQLVTSCGSDGHS--MALTETGEVFSWGDGDFGKLGHGNSERQRRPKQIEALQGEEVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLKHTLVLSLDGMVVWAFGDGDYGKLGTGSSTAKYYPQKVEQLCNKGIKKVCCGTQFSVALACDGHVYTFGQERLIGLPDSMLKNKSCPQVVPSLEGLFIEDIAVGCEHVLALSSTGDIYAWGCNSEGQLGLGHSNL-VKEPTFITSLQGKNIRQISAGRCHSAAWT 4301          

HSP 4 Score: 169.474 bits (428), Expect = 8.268e-38
Identity = 129/380 (33.95%), Postives = 180/380 (47.37%), Query Frame = 0

Query: 2540 PGGKSS---VYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFF--CGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            PG +S    VY+ G+  + QLA+     I  PK    F  +  +  G   TF++  +GS+ ACG+GSYG+LGLG+S++  S+   + L    N+                     VFSWGDGE GKLGHGN+   + PKII   + G     V+ G  HSA +T+ G+++T+G GD GRLGH + Q++  P  +  + G  V  VACG  HT                          N  +R  V          K+C G   SL LTS G VF   CG+   SG    S+      P+ I  L    ++DI+ G  H LAL+    V+ WG N +GQ G   +     +P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  376 PGSESDTVMVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQMIEAGQYCTFSVSADGSVKACGKGSYGRLGLGDSNNQ-SMPKKLVLEPHRNMKKVSSSKGSDGHTLAITAEGEVFSWGDGEYGKLGHGNSATQKYPKIIQGPMFGKVVVCVSAGYRHSAAVTNDGELYTWGEGDFGRLGHSDSQSRNVPTLVKDISG--VGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGSG----SAETTSLRPRFIEDLSITKIIDISCGDSHCLALTHENEVYAWGNNTMGQCGQGHTSTPITKPKKVLGLEGVSIQQITAGTSHSLAWTAVP 748          

HSP 5 Score: 106.686 bits (265), Expect = 8.702e-19
Identity = 89/299 (29.77%), Postives = 144/299 (48.16%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKX---------------------------FSSLISQSEKL-RGSIDNVLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWED 1551
            GY +A   I+ G   WK  I K+++G +   +GV+   +   N+ +  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  + +S ELYP V F S+    K+++ +L +++   S L   EP     +  + + T++++R L+  +   W S I+   H  +    EL+  LLK                               L+SQ   L    +  +   ++P+LAL+  VDSGL  G    +K   +R I++G+    S   K++WE+
Sbjct: 2025 GYGLATTAITSGCFIWKFHITKENRGNEGTCVGVSRWPVRDHNHHTTTDMWLYRAYSGNLYHG-GELVRTLPSFTQGDTITCILDMEAHTISFAKNDKDPKLAFEGVVAS-ELYPCVLFYSSNPGEKVALRDL-QMRGMPSNLLPGEPLCSPRTTVLLESTLQLLRRLH--RCDQWASHINQYIHTHL----ELIGPLLKDDELGSFNPGVGPTHFDKEEREREGGEDTEELLSQRRSLSEAKLAALCTEVWPVLALIGGVDSGLRAGGLCLHKPSGRRAILLGVLKEGSPLAKLQWEE 2314          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|768919362|ref|XP_011602888.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 isoform X4 [Takifugu rubripes])

HSP 1 Score: 790.03 bits (2039), Expect = 0.000e+0
Identity = 487/1266 (38.47%), Postives = 699/1266 (55.21%), Query Frame = 0

Query: 2192 FDDESTLEISVMSDQI--LTWSPSGKYLV-LSVGNIIYI-------WDMEDS--EENCNQIPQLSPIIYLCWSPNSEIEILLAAR----------QNGTSISITTLSWS--SNSDYLAIGYENGEILVLQIDQRKESIHLYGS-QIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP-------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
            +D+E  + IS+  D +  L W P+G  L+ L    I+ I       W    S    +   I +  P+  L  +P+  + + +  +          Q GT++S+  +  S  S      +  E+ E + +          L+G    V  L F  S   +++ G     GG++NIWS+   SV++T+ G G +   +WI +  +AV F  +++V L+    E    N ++AS CR  L  +++     AP    F+++    + EQ+ YE+  ++ G QL++S FLQ+L+ LA  L++   LC LP              S W WL+S+  ++     +       +  L+      D+ +    L +  W+ + D  LM W  V P DWQ GGKS VYL GNG+ GQLA  G++   P  A S   +  V+CG   +F +Q NG + A GEG YG+LG GNSDD +  T        VVT L++      H++       VFSWGDG+ GKLGHGN ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+G+ GRLG  +   K  PE++++L GY V  V+CGL HT                                 K+     + + K+C G  FS+ +   G V+  G  +  G+  +S   N  +P+ +P LE +   DIAVG +HVLALS +G V+ WGCN  GQLG       KEP +I  L GK I QISAG+ HS+AWT+P   T+ N+  P   +  +G P S+P ++ + L   S      RL++L+  S+ +   WRL  +         +      A+    +R  LSP V TLPL++ +  TMT     + +GPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL    ++LL  TPNG +D+GSN D++LLN    S+  M  F FLG+L  VA+RTRKP+ ++LAP VWK +  I     D+E+VDL   R+L  +    N    ED+   ++P D FM  S  GR VP+ PG + I L++ N+ +YV+  L+ R  EM  QV AVR+G+S + P P+L LL  Q +E LVCG     + +LKK+ RYRD+  S+++IVW W+ L+EF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFF LRLPPYTS+ I+  RL Y+I +C +IDMD+YMLT N E   + G D
Sbjct: 3545 YDNEQPIVISIFQDSVSSLKWDPTGHLLLCLGKSEIVKILGCYKGTWVTLHSLIHSSTVNISEWCPLAGL--APDPRLMMAVGCQNGSVYVWTLPQGGTAVSVPNIKSSPPSQDKCTDLKQESAECVFV----------LHGHITAVRSLSFCPSGLALVSGGI----GGLLNIWSLQDGSVLQTVTGLGSVLSTNWIPNMGVAVCFGRSKDVLLICCTPEWISQNHLLAS-CRMVLRSQNILGLNRAPCLVVFLERLPLLLQEQHSYERSHVAAGEQLVHSAFLQSLASLAVGLSIEKHLCHLPRPPHHRSSESDSCPSEWSWLASYATTVQTAEAIASGTVFPESFLMSEFQELDDAESAKALDNSKWSYKMDEQLMSWANVRPEDWQEGGKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPTIISAFQGYVVTQLVTSCGSDGHSMALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLP-DSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCNPVKEPTLITALQGKNIKQISAGRCHSSAWTTPS--TSKNSGGPGNLQ--LGLPQSVPPQY-NTLKDCSPRVLSMRLRVLYNFSDLMYKSWRLLNLDPQSPLSTSRYSSGTAAIIRGDLRGLLSPKVNTLPLVRCIGRTMTQG---KTHGPQITVKRISTRGRSSKPIFVQIAKQVVSLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGAVDLLIHTPNGFADVGSNTDRFLLNPAAFSEDHMIQFRFLGILMAVAIRTRKPLDLHLAPWVWKQLCSIPLGGADIEEVDLLTYRTLQGILHLENSGITEDNFHVMIPLDSFMVHSADGRLVPVVPGGQNIALTFANRTDYVERGLDYRLHEMDSQVAAVREGMSTIIPVPLLSLLTPQQLEQLVCGLPEVSVEMLKKLVRYRDVTESHQLIVWFWQSLQEFTNEERVLFLRFVSGRSRLPSNPADITQKFQIIKVDRPINGLPTAQTCFFLLRLPPYTSQAILSERLRYSIHNCPSIDMDNYMLTHNTEPADSSGTD 4784          

HSP 2 Score: 299.671 bits (766), Expect = 3.128e-77
Identity = 242/764 (31.68%), Postives = 371/764 (48.56%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHP---LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQI-------PQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAI--------------QNPSLTINPVIFNKAVQLAQ--------GRTYDSHTI--------YLKEAAMYLTEQIVHF----------------TSRNKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDIL--YNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGK 749
             + L+ +G++TGLSLLFSLLQQNW      +P   L + V++TA ++L S PPLSL     +P +G   L  VA FL  ++ I       P       ELLL LA+QRG    +L W+  AL  ++              Q  +     VI    +Q+ Q        G + ++  +         L +A + L E+I +                 T  +   +Y WG +            +  PK+    +D + IE G  CT+ + T+G V +C K ++        +N   P  ++          S S+ ++   LA+T D +V+SWG+G +G+LGHG+     +PK I    P     ++  SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++  VG V+CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ WG           T+S  P+++   SI+ I ++S G  H + +     VY+WG NT G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ + + L  Y  GL +           D QQ  LL +  L +      +    +     +   L +++F+ ID  N+   I   V+  + +GA  L P L++R      LL      +N LSKGQ+ +L M L SL +   I+ L+  S  G+
Sbjct:  162 TDVLIEMGVRTGLSLLFSLLQQNWRYAASAYPESVLCNDVLATACSVLASLPPLSLANENKIPSVGLECLAQVAEFLKRTSVISGAGAADPTGRRLALELLLGLAMQRGSLKFLLEWVEVALAASMSSSNSMPSSSAMTSQKSAGVGFDVIQQTLLQMRQYSFLQGFSGDSINTQVLKKDADGLCQLSQAVLCLFEEICNLASYCLCSSSTDAASPGTEGDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQSLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQG--PLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISGVGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVPTDRQLVAWHRPFCVDLEESTFTYLCNFLKCYCDGLENDTPPTPFLSKRDHQQFILLCMKLLSVHLSLAHAGGTGALVLGTQGRPLRNLLFRLIDS-NMPDSIQKAVLNTLSIGASLLLPPLRERTELLLSLLPRGPQSLNVLSKGQRLQLDMVLSSLQDQSYIASLLGYSSLGE 922          

HSP 3 Score: 173.326 bits (438), Expect = 6.147e-39
Identity = 124/416 (29.81%), Postives = 198/416 (47.60%), Query Frame = 0

Query:  260 NKQDIYTWG-----------CDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPIIDA-----------SLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWTM------RKGKGCFH--WPYSNEPDAKTFSQVSD--------ILYNF 613
             K D+Y WG            +L  P +   ++  +++  G +C+++I +NG V+   +  +         +++ P+ II A           S   D  S  +ALTE  +V+SWG+G  G+LGHG+ +    PK+I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG +      +P  V  L  ++VG VSCG  HTL++S DGM VW+FG G+ G+LG G +   Y P+ +       MKKV    +FS+ ++  G ++ +G+            + + P+ + S+    I +++ G  H++ +     VY+WG N+ G+ GLG  +  VK P     L   +I  I AG   S AWT         G G      P S  P   T    S         +LYNF
Sbjct: 3920 GKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPT-IISAFQGYVVTQLVTSCGSDGHS--MALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLPDSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCN-PVKEPTLITALQGKNIKQISAGRCHSSAWTTPSTSKNSGGPGNLQLGLPQSVPPQYNTLKDCSPRVLSMRLRVLYNF 4331          

HSP 4 Score: 167.548 bits (423), Expect = 3.766e-37
Identity = 126/374 (33.69%), Postives = 173/374 (46.26%), Query Frame = 0

Query: 2541 GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            G   +VY+ G+  + QLA+     I  PK    F  + ++  G   TF++  +GS+ ACG+GSYG+LGLG+S++  SL   + L    N+                     VFSWGDGE GKLGHGN+   + PKII   L        + G  HSA +T  G+++T+G GD GRLGH + Q++  P  +  + G  V  VACG  HT                          N  +R  V          K+C G   SL LTS G VF  G    S +   SS      P+ +  L    ++DI+ G  H LALS    V+ WG N +GQ G   +      P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  381 GDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQ-SLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQGPLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISG--VGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCG--SCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVP 749          

HSP 5 Score: 104.76 bits (260), Expect = 3.161e-18
Identity = 125/513 (24.37%), Postives = 224/513 (43.66%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKX-----FSSLIS-------QSEKLRGSIDN-------------------VLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWED-DGSSSFRSIQCLKLIKKIDSPVHKIFMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSL 1761
            GY +    I+ G   WK SI K+++G +   IGV+   +   N+ +  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  + +S ELYP V F S+    K+S+ +L +++   + L   EP     S  + + T++++R L+  +   W  +I+      +    EL+  LLK       SS +S       Q E+ + ++D                    +   ++P+LA++  VD+GL  G   ++    ++ +++G+    S   K++WE+ + S S   I  L+        V  +   + S+ L+D++ L+    E+  L+    +K +  +   +EN    S   +    S  +      +   +    V++ E       S  TLH       A +  +++    L          AL + +  + + +    + TP ++ S S     S  E+T        RNRQ  + + L + L
Sbjct: 2005 GYGLTTTAITSGCFIWKFSISKENRGNEGTCIGVSRWPVRDHNHHTTTDMWLYRAYSGNLYHG-GELIRTLPSFTQGDTITCILDMEAHTISFAKNDKEPKLAFEGVIAS-ELYPCVLFYSSNPGEKVSLHDL-QMRGMPNNLLPGEPLCSPRSTVLVESTIQLLRRLH--QCDQWAPLIN----QYIYSQLELIGPLLKEDDLSHSSSDVSPIHFEKEQREREKDTLDTDRGELYLHRRALSDAKLAALCSEVWPVLAVIGGVDTGLRAGGLCQHAPSGRKAVLLGVLKEGSSMAKLQWEETELSVSDTPITSLEPCSTSCFDVTPLGGLR-STVLLDLIYLMGLLEEQGWLATHQDHKIKCSQENMQENEIQRSNDEDGKEKSPETDSFSSSSSTRDPRKIVDHQESTCPTQPSQ-TLHSSKLDAFAFERRAVRVSYLLI--------GALKSLTHIFSSENPADLLQTPKHDPSNSSTTPLSSPEQTRE-----ERNRQWDERAELRSVL 2493          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|768919360|ref|XP_011602887.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 isoform X3 [Takifugu rubripes])

HSP 1 Score: 790.03 bits (2039), Expect = 0.000e+0
Identity = 487/1266 (38.47%), Postives = 699/1266 (55.21%), Query Frame = 0

Query: 2192 FDDESTLEISVMSDQI--LTWSPSGKYLV-LSVGNIIYI-------WDMEDS--EENCNQIPQLSPIIYLCWSPNSEIEILLAAR----------QNGTSISITTLSWS--SNSDYLAIGYENGEILVLQIDQRKESIHLYGS-QIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP-------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
            +D+E  + IS+  D +  L W P+G  L+ L    I+ I       W    S    +   I +  P+  L  +P+  + + +  +          Q GT++S+  +  S  S      +  E+ E + +          L+G    V  L F  S   +++ G     GG++NIWS+   SV++T+ G G +   +WI +  +AV F  +++V L+    E    N ++AS CR  L  +++     AP    F+++    + EQ+ YE+  ++ G QL++S FLQ+L+ LA  L++   LC LP              S W WL+S+  ++     +       +  L+      D+ +    L +  W+ + D  LM W  V P DWQ GGKS VYL GNG+ GQLA  G++   P  A S   +  V+CG   +F +Q NG + A GEG YG+LG GNSDD +  T        VVT L++      H++       VFSWGDG+ GKLGHGN ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+G+ GRLG  +   K  PE++++L GY V  V+CGL HT                                 K+     + + K+C G  FS+ +   G V+  G  +  G+  +S   N  +P+ +P LE +   DIAVG +HVLALS +G V+ WGCN  GQLG       KEP +I  L GK I QISAG+ HS+AWT+P   T+ N+  P   +  +G P S+P ++ + L   S      RL++L+  S+ +   WRL  +         +      A+    +R  LSP V TLPL++ +  TMT     + +GPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL    ++LL  TPNG +D+GSN D++LLN    S+  M  F FLG+L  VA+RTRKP+ ++LAP VWK +  I     D+E+VDL   R+L  +    N    ED+   ++P D FM  S  GR VP+ PG + I L++ N+ +YV+  L+ R  EM  QV AVR+G+S + P P+L LL  Q +E LVCG     + +LKK+ RYRD+  S+++IVW W+ L+EF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFF LRLPPYTS+ I+  RL Y+I +C +IDMD+YMLT N E   + G D
Sbjct: 3548 YDNEQPIVISIFQDSVSSLKWDPTGHLLLCLGKSEIVKILGCYKGTWVTLHSLIHSSTVNISEWCPLAGL--APDPRLMMAVGCQNGSVYVWTLPQGGTAVSVPNIKSSPPSQDKCTDLKQESAECVFV----------LHGHITAVRSLSFCPSGLALVSGGI----GGLLNIWSLQDGSVLQTVTGLGSVLSTNWIPNMGVAVCFGRSKDVLLICCTPEWISQNHLLAS-CRMVLRSQNILGLNRAPCLVVFLERLPLLLQEQHSYERSHVAAGEQLVHSAFLQSLASLAVGLSIEKHLCHLPRPPHHRSSESDSCPSEWSWLASYATTVQTAEAIASGTVFPESFLMSEFQELDDAESAKALDNSKWSYKMDEQLMSWANVRPEDWQEGGKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPTIISAFQGYVVTQLVTSCGSDGHSMALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLP-DSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCNPVKEPTLITALQGKNIKQISAGRCHSSAWTTPS--TSKNSGGPGNLQ--LGLPQSVPPQY-NTLKDCSPRVLSMRLRVLYNFSDLMYKSWRLLNLDPQSPLSTSRYSSGTAAIIRGDLRGLLSPKVNTLPLVRCIGRTMTQG---KTHGPQITVKRISTRGRSSKPIFVQIAKQVVSLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGAVDLLIHTPNGFADVGSNTDRFLLNPAAFSEDHMIQFRFLGILMAVAIRTRKPLDLHLAPWVWKQLCSIPLGGADIEEVDLLTYRTLQGILHLENSGITEDNFHVMIPLDSFMVHSADGRLVPVVPGGQNIALTFANRTDYVERGLDYRLHEMDSQVAAVREGMSTIIPVPLLSLLTPQQLEQLVCGLPEVSVEMLKKLVRYRDVTESHQLIVWFWQSLQEFTNEERVLFLRFVSGRSRLPSNPADITQKFQIIKVDRPINGLPTAQTCFFLLRLPPYTSQAILSERLRYSIHNCPSIDMDNYMLTHNTEPADSSGTD 4787          

HSP 2 Score: 300.056 bits (767), Expect = 2.975e-77
Identity = 242/764 (31.68%), Postives = 371/764 (48.56%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHP---LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQI-------PQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAI--------------QNPSLTINPVIFNKAVQLAQ--------GRTYDSHTI--------YLKEAAMYLTEQIVHF----------------TSRNKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDIL--YNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGK 749
             + L+ +G++TGLSLLFSLLQQNW      +P   L + V++TA ++L S PPLSL     +P +G   L  VA FL  ++ I       P       ELLL LA+QRG    +L W+  AL  ++              Q  +     VI    +Q+ Q        G + ++  +         L +A + L E+I +                 T  +   +Y WG +            +  PK+    +D + IE G  CT+ + T+G V +C K ++        +N   P  ++          S S+ ++   LA+T D +V+SWG+G +G+LGHG+     +PK I    P     ++  SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++  VG V+CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ WG           T+S  P+++   SI+ I ++S G  H + +     VY+WG NT G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ + + L  Y  GL +           D QQ  LL +  L +      +    +     +   L +++F+ ID  N+   I   V+  + +GA  L P L++R      LL      +N LSKGQ+ +L M L SL +   I+ L+  S  G+
Sbjct:  162 TDVLIEMGVRTGLSLLFSLLQQNWRYAASAYPESVLCNDVLATACSVLASLPPLSLANENKIPSVGLECLAQVAEFLKRTSVISGAGAADPTGRRLALELLLGLAMQRGSLKFLLEWVEVALAASMSSSNSMPSSSAMTSQKSAGVGFDVIQQTLLQMRQYSFLQGFSGDSINTQVLKKDADGLCQLSQAVLCLFEEICNLASYCLCSSSTDAASPGTEGDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQSLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQG--PLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISGVGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVPTDRQLVAWHRPFCVDLEESTFTYLCNFLKCYCDGLENDTPPTPFLSKRDHQQFILLCMKLLSVHLSLAHAGGTGALVLGTQGRPLRNLLFRLIDS-NMPDSIQKAVLNTLSIGASLLLPPLRERTELLLSLLPRGPQSLNVLSKGQRLQLDMVLSSLQDQSYIASLLGYSSLGE 922          

HSP 3 Score: 173.326 bits (438), Expect = 5.513e-39
Identity = 124/416 (29.81%), Postives = 198/416 (47.60%), Query Frame = 0

Query:  260 NKQDIYTWG-----------CDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPIIDA-----------SLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWTM------RKGKGCFH--WPYSNEPDAKTFSQVSD--------ILYNF 613
             K D+Y WG            +L  P +   ++  +++  G +C+++I +NG V+   +  +         +++ P+ II A           S   D  S  +ALTE  +V+SWG+G  G+LGHG+ +    PK+I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG +      +P  V  L  ++VG VSCG  HTL++S DGM VW+FG G+ G+LG G +   Y P+ +       MKKV    +FS+ ++  G ++ +G+            + + P+ + S+    I +++ G  H++ +     VY+WG N+ G+ GLG  +  VK P     L   +I  I AG   S AWT         G G      P S  P   T    S         +LYNF
Sbjct: 3923 GKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPT-IISAFQGYVVTQLVTSCGSDGHS--MALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLPDSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCN-PVKEPTLITALQGKNIKQISAGRCHSSAWTTPSTSKNSGGPGNLQLGLPQSVPPQYNTLKDCSPRVLSMRLRVLYNF 4334          

HSP 4 Score: 167.548 bits (423), Expect = 3.523e-37
Identity = 126/374 (33.69%), Postives = 173/374 (46.26%), Query Frame = 0

Query: 2541 GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            G   +VY+ G+  + QLA+     I  PK    F  + ++  G   TF++  +GS+ ACG+GSYG+LGLG+S++  SL   + L    N+                     VFSWGDGE GKLGHGN+   + PKII   L        + G  HSA +T  G+++T+G GD GRLGH + Q++  P  +  + G  V  VACG  HT                          N  +R  V          K+C G   SL LTS G VF  G    S +   SS      P+ +  L    ++DI+ G  H LALS    V+ WG N +GQ G   +      P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  381 GDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQ-SLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQGPLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISG--VGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCG--SCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVP 749          

HSP 5 Score: 104.76 bits (260), Expect = 3.296e-18
Identity = 123/524 (23.47%), Postives = 224/524 (42.75%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKX-----FSSLIS-------QSEKLRGSIDN-------------------VLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKI------------FMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSL 1761
            GY +    I+ G   WK SI K+++G +   IGV+   +   N+ +  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  + +S ELYP V F S+    K+S+ +L +++   + L   EP     S  + + T++++R L+  +   W  +I+      +    EL+  LLK       SS +S       Q E+ + ++D                    +   ++P+LA++  VD+GL  G   ++    ++ +++G+    S   K++WE+    +  S+  +      D+P+  +                 S+ L+D++ L+    E+  L+    +K +  +   +EN    S   +    S  +      +   +    V++ E       S  TLH       A +  +++    L          AL + +  + + +    + TP ++ S S     S  E+T        RNRQ  + + L + L
Sbjct: 2005 GYGLTTTAITSGCFIWKFSISKENRGNEGTCIGVSRWPVRDHNHHTTTDMWLYRAYSGNLYHG-GELIRTLPSFTQGDTITCILDMEAHTISFAKNDKEPKLAFEGVIAS-ELYPCVLFYSSNPGEKVSLHDL-QMRGMPNNLLPGEPLCSPRSTVLVESTIQLLRRLH--QCDQWAPLIN----QYIYSQLELIGPLLKEDDLSHSSSDVSPIHFEKEQREREKDTLDTDRGELYLHRRALSDAKLAALCSEVWPVLAVIGGVDTGLRAGGLCQHAPSGRKAVLLGVLKEGSSMAKLQWEE----TELSVSSMNSWSPSDTPITSLEPCSTSCFDVTPLGGLRSTVLLDLIYLMGLLEEQGWLATHQDHKIKCSQENMQENEIQRSNDEDGKEKSPETDSFSSSSSTRDPRKIVDHQESTCPTQPSQ-TLHSSKLDAFAFERRAVRVSYLLI--------GALKSLTHIFSSENPADLLQTPKHDPSNSSTTPLSSPEQTRE-----ERNRQWDERAELRSVL 2501          

BLAST of EMLSAG00000002245 vs. nr
Match: gi|768919358|ref|XP_011602886.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC1 isoform X2 [Takifugu rubripes])

HSP 1 Score: 790.03 bits (2039), Expect = 0.000e+0
Identity = 487/1266 (38.47%), Postives = 699/1266 (55.21%), Query Frame = 0

Query: 2192 FDDESTLEISVMSDQI--LTWSPSGKYLV-LSVGNIIYI-------WDMEDS--EENCNQIPQLSPIIYLCWSPNSEIEILLAAR----------QNGTSISITTLSWS--SNSDYLAIGYENGEILVLQIDQRKESIHLYGS-QIVSYLLFHKSDPYILASGSSKNPGGIVNIWSILSQSVIRTLIGDGGITCLHWINSKTLAVGFCNTENVTLMNLPNE--MDNVIIASKCRAELF-EHLPFEQFAPYFRAFIKQFGYFIIEQYEYEKLCLSEGFQLIYSQFLQNLSFLAFVLNLNTVLCSLP-------------DSRWKWLSSFTQSMTFISLLNKRNAKSDHELLKSLF-FDEIKKDVNLKDIVWTPEADSHLMKWYIVYPSDWQPGGKSSVYLVGNGQNGQLADLGSSSIPPKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTP-------VVTLLLS------HNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------------------------------KINFAWRESVTKICLGRTFSLFLT-SGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGSSS--IFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDS---LLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNNETRPTGGGD 3356
            +D+E  + IS+  D +  L W P+G  L+ L    I+ I       W    S    +   I +  P+  L  +P+  + + +  +          Q GT++S+  +  S  S      +  E+ E + +          L+G    V  L F  S   +++ G     GG++NIWS+   SV++T+ G G +   +WI +  +AV F  +++V L+    E    N ++AS CR  L  +++     AP    F+++    + EQ+ YE+  ++ G QL++S FLQ+L+ LA  L++   LC LP              S W WL+S+  ++     +       +  L+      D+ +    L +  W+ + D  LM W  V P DWQ GGKS VYL GNG+ GQLA  G++   P  A S   +  V+CG   +F +Q NG + A GEG YG+LG GNSDD +  T        VVT L++      H++       VFSWGDG+ GKLGHGN ER RRPK I +L G     +A G  HSAV+T  GK+FTFG+G+ GRLG  +   K  PE++++L GY V  V+CGL HT                                 K+     + + K+C G  FS+ +   G V+  G  +  G+  +S   N  +P+ +P LE +   DIAVG +HVLALS +G V+ WGCN  GQLG       KEP +I  L GK I QISAG+ HS+AWT+P   T+ N+  P   +  +G P S+P ++ + L   S      RL++L+  S+ +   WRL  +         +      A+    +R  LSP V TLPL++ +  TMT     + +GPQ+TV+R+   G SS  IF QIA+QV  ++P ELRLP R+WKVKLVGE ADDAGGVFDDTI+EMCQEL    ++LL  TPNG +D+GSN D++LLN    S+  M  F FLG+L  VA+RTRKP+ ++LAP VWK +  I     D+E+VDL   R+L  +    N    ED+   ++P D FM  S  GR VP+ PG + I L++ N+ +YV+  L+ R  EM  QV AVR+G+S + P P+L LL  Q +E LVCG     + +LKK+ RYRD+  S+++IVW W+ L+EF N+ ++LFL FVSGRSRLP++  D++Q+FQI++V+RP NGLPTAQTCFF LRLPPYTS+ I+  RL Y+I +C +IDMD+YMLT N E   + G D
Sbjct: 3550 YDNEQPIVISIFQDSVSSLKWDPTGHLLLCLGKSEIVKILGCYKGTWVTLHSLIHSSTVNISEWCPLAGL--APDPRLMMAVGCQNGSVYVWTLPQGGTAVSVPNIKSSPPSQDKCTDLKQESAECVFV----------LHGHITAVRSLSFCPSGLALVSGGI----GGLLNIWSLQDGSVLQTVTGLGSVLSTNWIPNMGVAVCFGRSKDVLLICCTPEWISQNHLLAS-CRMVLRSQNILGLNRAPCLVVFLERLPLLLQEQHSYERSHVAAGEQLVHSAFLQSLASLAVGLSIEKHLCHLPRPPHHRSSESDSCPSEWSWLASYATTVQTAEAIASGTVFPESFLMSEFQELDDAESAKALDNSKWSYKMDEQLMSWANVRPEDWQEGGKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPTIISAFQGYVVTQLVTSCGSDGHSMALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLP-DSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCNPVKEPTLITALQGKNIKQISAGRCHSSAWTTPS--TSKNSGGPGNLQ--LGLPQSVPPQY-NTLKDCSPRVLSMRLRVLYNFSDLMYKSWRLLNLDPQSPLSTSRYSSGTAAIIRGDLRGLLSPKVNTLPLVRCIGRTMTQG---KTHGPQITVKRISTRGRSSKPIFVQIAKQVVSLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGAVDLLIHTPNGFADVGSNTDRFLLNPAAFSEDHMIQFRFLGILMAVAIRTRKPLDLHLAPWVWKQLCSIPLGGADIEEVDLLTYRTLQGILHLENSGITEDNFHVMIPLDSFMVHSADGRLVPVVPGGQNIALTFANRTDYVERGLDYRLHEMDSQVAAVREGMSTIIPVPLLSLLTPQQLEQLVCGLPEVSVEMLKKLVRYRDVTESHQLIVWFWQSLQEFTNEERVLFLRFVSGRSRLPSNPADITQKFQIIKVDRPINGLPTAQTCFFLLRLPPYTSQAILSERLRYSIHNCPSIDMDNYMLTHNTEPADSSGTD 4789          

HSP 2 Score: 301.982 bits (772), Expect = 6.354e-78
Identity = 242/761 (31.80%), Postives = 369/761 (48.49%), Query Frame = 0

Query:   99 NEALLNLGLQTGLSLLFSLLQQNWSGPDEQHP---LSSTVISTANNMLLSFPPLSLL---AVPPLGETALDNVATFLHTSAQI-------PQASTSCYELLLSLAIQRGRCSDVLHWINTALTVAI--------------QNPSLTINPVIFNKAVQLAQGRTYDSHTI-------------YLKEAAMYLTEQIVHF----------------TSRNKQDIYTWGCD------------LHSPKIWDDVNDVEKIETGSSCTYVIHTNGKV-SCWKDNHF-------SNVHFPSPII--------DASLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW------NTSSTVPKWIN--SISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPKR---LDIYDIDGICAGPTQSYAWTM---RKGKGCFHWPYSNEPDAKTFSQVSDIL--YNFGLSDFNAKLNSHFDEDEQQKFLLHIFTLLISYFETISSSIRSCDWSAEKVKLEDIIFKFIDKENISPEIYCKVIKCIEVGAQFLQPQLKDRVRRCYKLL-----KMNNLSKGQQYKLKMDLESLNEPGIISGLICESEEGK 749
             + L+ +G++TGLSLLFSLLQQNW      +P   L + V++TA ++L S PPLSL     +P +G   L  VA FL  ++ I       P       ELLL LA+QRG    +L W+  AL  ++              Q  +     VI    +Q+ Q   +   +I              L +A + L E+I +                 T  +   +Y WG +            +  PK+    +D + IE G  CT+ + T+G V +C K ++        +N   P  ++          S S+ ++   LA+T D +V+SWG+G +G+LGHG+     +PK I    P     ++  SAG+ H+AA+T DG LYTWGEG  GRLGH D     +PTLVK++  VG V+CG+ HT+ V++DG  VWSFG G+NG+LG GDT  +Y PK+I    G I++KV A ++ SLAL+ +G ++ WG           T+S  P+++   SI+ I ++S G  H + +     VY+WG NT G+CG G TS  +  PK+   L+   I  I AG + S AWT     +    +H P+  + +  TF+ + + L  Y  GL +           D QQ  LL +  L +      +    +     +   L +++F+ ID  N+   I   V+  + +GA  L P L++R      LL      +N LSKGQ+ +L M L SL +   I+ L+  S  G+
Sbjct:  162 TDVLIEMGVRTGLSLLFSLLQQNWRYAASAYPESVLCNDVLATACSVLASLPPLSLANENKIPSVGLECLAQVAEFLKRTSVISGAGAADPTGRRLALELLLGLAMQRGSLKFLLEWVEVALAASMSSSNSMPSSSAMTSQKSAGVGFDVIQQTLLQMRQYSGFSGDSINTQVLKKDADGLCQLSQAVLCLFEEICNLASYCLCSSSTDAASPGTEGDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQSLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQG--PLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISGVGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVPTDRQLVAWHRPFCVDLEESTFTYLCNFLKCYCDGLENDTPPTPFLSKRDHQQFILLCMKLLSVHLSLAHAGGTGALVLGTQGRPLRNLLFRLIDS-NMPDSIQKAVLNTLSIGASLLLPPLRERTELLLSLLPRGPQSLNVLSKGQRLQLDMVLSSLQDQSYIASLLGYSSLGE 919          

HSP 3 Score: 173.326 bits (438), Expect = 6.200e-39
Identity = 124/416 (29.81%), Postives = 198/416 (47.60%), Query Frame = 0

Query:  260 NKQDIYTWG-----------CDLHSPKIWDDVNDVEKIETGSSCTYVIHTNGKVSCWKDNHFS--------NVHFPSPIIDA-----------SLSQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKT-------PWNTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNP---KRLDIYDIDGICAGPTQSYAWTM------RKGKGCFH--WPYSNEPDAKTFSQVSD--------ILYNF 613
             K D+Y WG            +L  P +   ++  +++  G +C+++I +NG V+   +  +         +++ P+ II A           S   D  S  +ALTE  +V+SWG+G  G+LGHG+ +    PK+I +L    ++ ++ G  H+A +T DG L+T+G G  GRLG +      +P  V  L  ++VG VSCG  HTL++S DGM VW+FG G+ G+LG G +   Y P+ +       MKKV    +FS+ ++  G ++ +G+            + + P+ + S+    I +++ G  H++ +     VY+WG N+ G+ GLG  +  VK P     L   +I  I AG   S AWT         G G      P S  P   T    S         +LYNF
Sbjct: 3925 GKSDVYLWGNGRYGQLAGTGTNLTVPTLAPSLSQTQQVVCGQNCSFLIQSNGVVTAVGEGQYGRLGQGNSDDLYVPT-IISAFQGYVVTQLVTSCGSDGHS--MALTETGEVFSWGDGDFGKLGHGNNERQRRPKQIEALQGEDVVQLACGFRHSAVVTADGKLFTFGSGESGRLGQRSTSNKMLPERVAALEGYHVGQVSCGLNHTLVLSLDGMAVWAFGDGDYGKLGAGSSTAKYYPQKVEQLCNKGMKKVCCGAQFSVVVACDGHVYTFGQERLIGLPDSMLKNRSNPQVVPSLEGLFIEDIAVGCEHVLALSSTGDVYAWGCNSEGQLGLGHCN-PVKEPTLITALQGKNIKQISAGRCHSSAWTTPSTSKNSGGPGNLQLGLPQSVPPQYNTLKDCSPRVLSMRLRVLYNF 4336          

HSP 4 Score: 167.548 bits (423), Expect = 3.643e-37
Identity = 126/374 (33.69%), Postives = 173/374 (46.26%), Query Frame = 0

Query: 2541 GGKSSVYLVGNGQNGQLADLGSSSIP-PKYADSFGGSSSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVX-------------------VFSWGDGEDGKLGHGNTERCRRPKIISS-LSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT------------------------KINFAWRESV---------TKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE--NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLG-NESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPP 2856
            G   +VY+ G+  + QLA+     I  PK    F  + ++  G   TF++  +GS+ ACG+GSYG+LGLG+S++  SL   + L    N+                     VFSWGDGE GKLGHGN+   + PKII   L        + G  HSA +T  G+++T+G GD GRLGH + Q++  P  +  + G  V  VACG  HT                          N  +R  V          K+C G   SL LTS G VF  G    S +   SS      P+ +  L    ++DI+ G  H LALS    V+ WG N +GQ G   +      P  +  L G  I QI+AG +HS AWT+ P
Sbjct:  378 GDAVTVYVWGSNSSHQLAEGTLEKILLPKLTQGFSDAQTIEAGQYCTFSVSTDGSVKACGKGSYGRLGLGDSNNQ-SLPKKLVLEPHRNIKKVSSSKGSDGHTLAITVDGEVFSWGDGEYGKLGHGNSATQKYPKIIQGPLFSKIVVSASAGYRHSAAVTKDGELYTWGEGDFGRLGHSDSQSRNMPTLVKDISG--VGQVACGSSHTIAVAQDGRTVWSFGGGDNGKLGHGDTNRVYRPKVIEALHGFIIRKVCAGSQSSLALTSAGQVFAWGCG--SCLGCGSSETTSLRPRFVEDLSITKIIDISCGDSHCLALSHENEVYAWGNNTMGQCGQGHTSTPITRPKKVLGLEGVSIQQITAGTSHSLAWTAVP 746          

HSP 5 Score: 104.76 bits (260), Expect = 3.380e-18
Identity = 123/524 (23.47%), Postives = 224/524 (42.75%), Query Frame = 0

Query: 1288 GYCMANRCISRGLVYWKISILKDSKGVDAISIGVTPGII---NNRSIKDMWLYMS-SGQLSHKEGIFSAHLPSFSKGDIIVCILNKEDSSLSFSKNGGPTLLAFCDLPSSIELYPVVSFSSTKA--KISISNLYELQPSTSTLYLTEPHLGSLSISIAQETVEIIRSLYNGKSQMWRSIIDSCSHNIVLKSYELVKDLLKX-----FSSLIS-------QSEKLRGSIDN-------------------VLEAIYPLLALLSDVDSGLCLGSYVRYK-EFKRGIIIGMGWGESINVKIRWEDDGSSSFRSIQCLKLIKKIDSPVHKI------------FMYKYSSELIDMLLLIYTFSEKLNLSVFSSYKSEEERNKNKENPASTSQAVENMTDSLVSSIIDEVTGKSESGNKVNNCEGNIEKSDSDVTLHEVFFKVIALQFSSIKAMTYLANNYESNASNALYNESQFYEAVSMIRRMSTPSYNISFSRLINRSEIERTFSILNNLRRNRQGSQMSSLDNSL 1761
            GY +    I+ G   WK SI K+++G +   IGV+   +   N+ +  DMWLY + SG L H  G     LPSF++GD I CIL+ E  ++SF+KN     LAF  + +S ELYP V F S+    K+S+ +L +++   + L   EP     S  + + T++++R L+  +   W  +I+      +    EL+  LLK       SS +S       Q E+ + ++D                    +   ++P+LA++  VD+GL  G   ++    ++ +++G+    S   K++WE+    +  S+  +      D+P+  +                 S+ L+D++ L+    E+  L+    +K +  +   +EN    S   +    S  +      +   +    V++ E       S  TLH       A +  +++    L          AL + +  + + +    + TP ++ S S     S  E+T        RNRQ  + + L + L
Sbjct: 2002 GYGLTTTAITSGCFIWKFSISKENRGNEGTCIGVSRWPVRDHNHHTTTDMWLYRAYSGNLYHG-GELIRTLPSFTQGDTITCILDMEAHTISFAKNDKEPKLAFEGVIAS-ELYPCVLFYSSNPGEKVSLHDL-QMRGMPNNLLPGEPLCSPRSTVLVESTIQLLRRLH--QCDQWAPLIN----QYIYSQLELIGPLLKEDDLSHSSSDVSPIHFEKEQREREKDTLDTDRGELYLHRRALSDAKLAALCSEVWPVLAVIGGVDTGLRAGGLCQHAPSGRKAVLLGVLKEGSSMAKLQWEE----TELSVSSMNSWSPSDTPITSLEPCSTSCFDVTPLGGLRSTVLLDLIYLMGLLEEQGWLATHQDHKIKCSQENMQENEIQRSNDEDGKEKSPETDSFSSSSSTRDPRKIVDHQESTCPTQPSQ-TLHSSKLDAFAFERRAVRVSYLLI--------GALKSLTHIFSSENPADLLQTPKHDPSNSSTTPLSSPEQTRE-----ERNRQWDERAELRSVL 2498          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold259_size234575-snap-gene-1.26 (protein:Tk11157 transcript:maker-scaffold259_size234575-snap-gene-1.26-mRNA-1 annotation:"e3 ubiquitin-protein ligase herc1")

HSP 1 Score: 668.307 bits (1723), Expect = 0.000e+0
Identity = 360/773 (46.57%), Postives = 489/773 (63.26%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFAT----------FVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT---------KINFAWRE------------------------SVTKICLGRTFSLFLTS-GDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLE----NMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTSPPMPTNFNNSSPTKPRHNIGRPNSLPDEFVSLLPTTSAXECLTRLKLLHEISNYIVNVWRLFPVIDSDFYWPPKIKDFYIAMSNDAVRESLSPLVATLPLMQTLMSTMTNSNVLRNYGPQVTVRRLFPIGS-------SSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQEL--SKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSYMLTRNN 3347
            VFSWGDG+ GKLGHGN++R RRP+ I++L  +  +          FVA G  HSA+++ +G ++TFG GD G LGHG   T + P  ++ L    +  VACG  HT           +F   E                         V K+ LG  FS+FLTS G V  CG  K +G+    +N++   P +IP  E     +VDI VG  H LALS  G VW WG + L         +  +P I+ +LS K ILQISAG  HS AWT+PP+   F+++ P  P  N+G P ++P +F +LL   S      RLK+L+  SN +   W+ +P ++         ++    ++ D +R  LS  V TLPL+Q L  TMT+S   RN  PQV VRRL             +IF+QIA+QV  ++P ELR   R+WKVKL+GE ADDAGGVFDD ++EMC+EL  +K  L LL PTPNG+++IG N+D +LLNS K+S  D+R+FWF+G+LCGVA+RTRKPIA+NLAP VWK+I+  + TW DL++VDL+Y+RSL  +    +P   E+ LLP +YF GI+F+G+ V L P  E++ L  ++  +YV+AA  QR +EM+PQV A+R+GL  + P+PIL L+P++ +E LVCG+ S  L  LKK ARYRD DPS+ +IVW W+IL+ F  + ++LF+IFVSGRSRLP +  D+ QRFQILRV+RPENGLPTAQTCFFQLRLPP+TS+ IM+ +L YAI HCR+IDMD YML R++
Sbjct:   31 VFSWGDGDWGKLGHGNSDRVRRPRPITTLRHHSGSDPQDSALNIRFVATGFRHSALISKEGHVYTFGKGDLGALGHGTTSTLRQPHLVADLTRRKIGLVACGSNHTVCVSRDGLQSWSFGGNECGQLGIGSTAGALSPKRIESLNGLQVKKVSLGDAFSVFLTSKGQVLTCGQHKMTGLPQILTNLH--RPTQIPAFELEHVFIVDITVGATHTLALSNKGEVWIWGRDGL----ESPDAWVLQPRIVADLSHKFILQISAGSYHSIAWTTPPLQPTFDDT-PHLP--NLGLPAAVPPKF-TLLANISIHALRDRLKVLYLASNLVAQSWKFYPSVED--------RNLLEPLARDEIRLILSQKVYTLPLVQILQKTMTSS---RNLWPQVVVRRLHLPSEHNDENCPKTIFAQIAKQVQKLEPAELRFQDRAWKVKLMGEGADDAGGVFDDIMTEMCRELIDAKSGLQLLIPTPNGVTNIGLNRDTFLLNSRKSSSHDLRNFWFIGILCGVAIRTRKPIALNLAPFVWKMIVGSQITWNDLDEVDLNYARSLKSLD---DPHLAENHLLPNEYFQGIAFAGQMVSLLP--EQLVLKPQDCPDYVQAAFEQRIREMLPQVKALREGLGGIVPFPILGLMPAKALEELVCGQPSISLTALKKAARYRDFDPSHPIIVWFWDILDHFTEEERVLFVIFVSGRSRLPNNLADLPQRFQILRVDRPENGLPTAQTCFFQLRLPPFTSQAIMQEKLRYAIYHCRSIDMDQYMLRRDS 777          

HSP 2 Score: 131.724 bits (330), Expect = 9.533e-31
Identity = 91/285 (31.93%), Postives = 138/285 (48.42%), Query Frame = 0

Query:  325 SQDNESFILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL-------PP-----IMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL--HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGK----------TPWNTSSTVPKWINSISSISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLGLTSLKVKNPK---RLDIYDIDGICAGPTQSYAWT 582
            S+  E   LAL    +V+SWG+G  G+LGHG+      P+ I++L       P      I  ++ G  H+A I+ +G++YT+G+G LG LGH        P LV +L    +G V+CG+ HT+ VS+DG+Q WSFG    G+LG G T    +PK I   +G+ +KKV   + FS+ L+  G +   G+          T  +  + +P +      I +++ G  H + +     V+ W     G+ GL      V  P+    L    I  I AG   S AWT
Sbjct:   15 SKSGEGHTLALATSGEVFSWGDGDWGKLGHGNSDRVRRPRPITTLRHHSGSDPQDSALNIRFVATGFRHSALISKEGHVYTFGKGDLGALGHGTTSTLRQPHLVADLTRRKIGLVACGSNHTVCVSRDGLQSWSFGGNECGQLGIGSTAGALSPKRIESLNGLQVKKVSLGDAFSVFLTSKGQVLTCGQHKMTGLPQILTNLHRPTQIPAFELEHVFIVDITVGATHTLALSNKGEVWIW-----GRDGLESPDAWVLQPRIVADLSHKFILQISAGSYHSIAWT 294          

HSP 3 Score: 68.9366 bits (167), Expect = 2.352e-11
Identity = 51/197 (25.89%), Postives = 84/197 (42.64%), Query Frame = 0

Query: 2671 GTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYF----------VTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL--ENMVDIAVGGDHVLALSKSGF-VWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAAWTS 2854
            G  H+  L   G++F++G+GD G+LGHGN    + P  I++LR +           + +VA G  H+                   +L    G V+  G      +  + +   ++ P  +  L    +  +A G +H + +S+ G   W +G N  GQLG  S      P  IE L+G  + ++S G   S   TS
Sbjct:   18 GEGHTLALATSGEVFSWGDGDWGKLGHGNSDRVRRPRPITTLRHHSGSDPQDSALNIRFVATGFRHS-------------------ALISKEGHVYTFGKGDLGALG-HGTTSTLRQPHLVADLTRRKIGLVACGSNHTVCVSRDGLQSWSFGGNECGQLGIGSTAGALSPKRIESLNGLQVKKVSLGDAFSVFLTS 194          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold732_size105256-snap-gene-0.22 (protein:Tk09377 transcript:maker-scaffold732_size105256-snap-gene-0.22-mRNA-1 annotation:"hypothetical protein SINV_14428")

HSP 1 Score: 208.379 bits (529), Expect = 7.265e-59
Identity = 122/328 (37.20%), Postives = 182/328 (55.49%), Query Frame = 0

Query: 3034 MCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSD-------------KNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPL---YPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---FQILRVERPENG-LPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMDSY 3341
            MC EL    L+LL PTPNG  + G+++D ++ N                ++ V    F FLG+L G+A+R+  P+++NLA  +WKL+   K T  D+ ++D  Y   L Y     +PD++  +++    F   S  G EV L   YP     +++ EN+ EYVK AL  R  E    V  VR+G++ + P P+L L     +E +VCG    PL +LK +A Y+ +D ++ ++ W W+++EEF+N  + LFL FV GR+RLP    D   R    QI+    P N  LP + TCFF L++P YT KEI+R +L YAI  C++ID D Y
Sbjct:    1 MCDELQNGSLSLLIPTPNGRDESGTSRDCFVFNPSMSVLLERGSAKGLASNNVQTNAFRFLGLLMGIAIRSGSPLSLNLAEPMWKLLAGSKLTPNDITEIDKDYVPGL-YCIRDMDPDSKAFAVMDMP-FSTPSVDGHEVNLSARYP-----KVTSENRGEYVKLALQYRLHEFDEVVALVREGMARVVPVPLLSLFTGFELEMMVCGSPDIPLHLLKSVATYKGVDHNSNLVEWFWDVMEEFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVLQIMDKYHPPNEFLPESYTCFFLLKMPRYTCKEILREKLKYAIHFCKSIDTDDY 321          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold259_size234575-snap-gene-1.21 (protein:Tk11160 transcript:maker-scaffold259_size234575-snap-gene-1.21-mRNA-1 annotation:"probable e3 ubiquitin-protein ligase herc1-like")

HSP 1 Score: 134.42 bits (337), Expect = 3.259e-36
Identity = 64/144 (44.44%), Postives = 95/144 (65.97%), Query Frame = 0

Query:  352 LGHGDKKDYNFPKEIS---SLPPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSG 492
            LGHG+      PK++        I+ ISAG+ H+AA++ +G+L+TWGEG LGRLGH D     +PT VK++  V  V+CGA HTL +S+D   VWSFG G++G+LG G+   +Y PK+I+   GV ++K+ A ++ S+ LS +G
Sbjct:    1 LGHGNTTTQKVPKKVVGPFERRTILQISAGYRHSAAVSEEGHLFTWGEGDLGRLGHGDSQSRNIPTQVKDIGLVRQVACGASHTLALSEDSQTVWSFGSGDHGKLGHGEIARIYRPKIIDALQGVHIEKIAAGSQVSMVLSRNG 144          

HSP 2 Score: 69.3218 bits (168), Expect = 1.591e-13
Identity = 34/86 (39.53%), Postives = 50/86 (58.14%), Query Frame = 0

Query: 2643 LGHGNTERCRRPK-IISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHT 2727
            LGHGNT   + PK ++          ++ G  HSA ++++G +FT+G GD GRLGHG+ Q++  P Q+  +    V  VACG  HT
Sbjct:    1 LGHGNTTTQKVPKKVVGPFERRTILQISAGYRHSAAVSEEGHLFTWGEGDLGRLGHGDSQSRNIPTQVKDIG--LVRQVACGASHT 84          

HSP 3 Score: 68.9366 bits (167), Expect = 2.553e-13
Identity = 31/109 (28.44%), Postives = 64/109 (58.72%), Query Frame = 0

Query:  334 ALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSLPPIMCISAGHHHAAAITLDGY-LYTWGEGSLGRLGHKDGIVHFVPTLVKNLHNVG--SVSCGAQHTLIVSKDG 439
            A++E+  +++WG G  GRLGHGD +  N P ++  +  +  ++ G  H  A++ D   ++++G G  G+LGH +    + P ++  L  V    ++ G+Q ++++S++G
Sbjct:   36 AVSEEGHLFTWGEGDLGRLGHGDSQSRNIPTQVKDIGLVRQVACGASHTLALSEDSQTVWSFGSGDHGKLGHGEIARIYRPKIIDALQGVHIEKIAAGSQVSMVLSRNG 144          

HSP 4 Score: 67.0106 bits (162), Expect = 1.066e-12
Identity = 32/93 (34.41%), Postives = 54/93 (58.06%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLT-DKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACG 2723
            +F+WG+G+ G+LGHG+++    P  +  +       VA G SH+  L+ D   +++FG+GD G+LGHG       P+ I +L+G  +  +A G
Sbjct:   43 LFTWGEGDLGRLGHGDSQSRNIPTQVKDIG--LVRQVACGASHTLALSEDSQTVWSFGSGDHGKLGHGEIARIYRPKIIDALQGVHIEKIAAG 133          

HSP 5 Score: 64.3142 bits (155), Expect = 9.000e-12
Identity = 45/137 (32.85%), Postives = 63/137 (45.99%), Query Frame = 0

Query: 2562 SSSIPPKYADSFGGSS--SVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVV--------------TLLLSHNLVXVFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKG 2682
            +  +P K    F   +   +  G + + A+   G L+  GEG  G+LG G+S      T V               TL LS +   V+S+G G+ GKLGHG   R  RPKII +L G     +A G+  S VL+  G
Sbjct:    8 TQKVPKKVVGPFERRTILQISAGYRHSAAVSEEGHLFTWGEGDLGRLGHGDSQSRNIPTQVKDIGLVRQVACGASHTLALSEDSQTVWSFGSGDHGKLGHGEIARIYRPKIIDALQGVHIEKIAAGSQVSMVLSRNG 144          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold56_size446035-snap-gene-3.36 (protein:Tk11506 transcript:maker-scaffold56_size446035-snap-gene-3.36-mRNA-1 annotation:"e3 ubiquitin-protein ligase smurf1 isoform x1")

HSP 1 Score: 113.235 bits (282), Expect = 6.823e-25
Identity = 95/379 (25.07%), Postives = 173/379 (45.65%), Query Frame = 0

Query: 2968 LRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSG--REVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331
            L++  PQ    RL  +    +F +   Q+  + PK+L+   +   +K  GE   D GG+  + +  +  E+      L            S  D Y L  + +S ++  H   F F+G + G+A+     I        +K++L       D+E+VD +   SLH++      +N+   +L +++ +     G  +   L  G + + ++ +NK EYVK  +N R+ + +  Q LA++KG + L P  +LK    + +E ++ G     +   K   R +  +    V+ W WEI++ ++ +++   L FV+G SR+P   F   Q          F +  +E     LP A TCF +L LP Y S+  M ++L  A++
Sbjct:  612 LQSQQPQSGHCRLE-VSRQEVFEESYRQIVKMRPKDLK---KRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQ---------YSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWML-----ENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVE 972          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold186_size273091-processed-gene-0.4 (protein:Tk00902 transcript:snap_masked-scaffold186_size273091-processed-gene-0.4-mRNA-1 annotation:"e3 ubiquitin-protein ligase nedd-4")

HSP 1 Score: 110.153 bits (274), Expect = 9.268e-24
Identity = 103/380 (27.11%), Postives = 180/380 (47.37%), Query Frame = 0

Query: 2973 PQVTVRRLFPIGSSSIFSQIAEQVFYI---DPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKV----DMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRD-MDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTID 3337
            P+VT+    P+    IF    E++  I   DP  L+   R W V+  GE+  D GG+      E    LSK++ N       G+ +  +N D Y L  +  S +     + +F F+G + G+A+     +        +K++L+ K T  D+E VD+ Y  SL Y+    +P+  E S    +  +G++ S     L P    + ++  NK +Y+   ++ RF   +  Q+ A ++G     P   L +     +E L+CG  +  +    K   YR    P+++V+ W W  +  F+N ++   L FV+G SR+P + F      + ++ F I     P++  P + TCF +L LP YTS + +R+RL+ A+++    D
Sbjct: 1234 PKVTI----PVRRDHIFQDSFERIRTIAQSDPNHLKA--RLW-VEFEGESGLDFGGL----AREWFFLLSKEMFNPYY----GLFEYSANDD-YTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIK-DHDPEPLELSFQVDEDRLGVTVSED---LIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDS-FPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFD 1592          

HSP 2 Score: 103.99 bits (258), Expect = 6.556e-22
Identity = 77/248 (31.05%), Postives = 125/248 (50.40%), Query Frame = 0

Query: 3077 HFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPY 3316
            +F F+G + G+A+   K +        +K++L    T KD+E VD  Y  SL ++  + +P   E +    +   G + S RE  L P   KI ++ E+K++Y+K  +  RF   +  Q+   + G + L P  +LK+     +E L+CG  S  +   K    Y+ D  P++ VI W W ++  FNN+++   L FV+G SR+P + F      +  Q + I +   P N  P A TCF +L LPPY
Sbjct:  576 YFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKEN-DPAELELTFQVDEEQFGTTIS-RE--LKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNP-NSFPRAHTCFNRLDLPPY 818          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold450_size166944-processed-gene-0.5 (protein:Tk00124 transcript:snap_masked-scaffold450_size166944-processed-gene-0.5-mRNA-1 annotation:"e3 ubiquitin-protein ligase huwe1-like isoform x5")

HSP 1 Score: 104.375 bits (259), Expect = 4.663e-22
Identity = 96/364 (26.37%), Postives = 154/364 (42.31%), Query Frame = 0

Query: 2994 EQVFYIDPKEL-RLPYRSWKVKLV----GEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREV------PLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSA-------LAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDV------SQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHC 3333
            E VF    +EL R     WK +      GE   DAGG+  +    + +E+   +  L   +P      G      + +  + +   + +F F+G +   A+   K +        +K IL     + D+E  D S+ + L ++         E ++    Y +  S   RE        L P    I +S + K EYV      R    M    A+RK LSA       + P  ++ +   Q +E L+ G  +  +  LK  + Y     ++  IVW W  L  F+   K  F+ FV+G S++P   F         Q+FQI R +R  + LP+A TCF QL LP Y + + +R+ L+ AI+ C
Sbjct: 1061 ENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSP------GDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLI--------EHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYV------RLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQEC 1404          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold495_size155559-snap-gene-0.44 (protein:Tk09247 transcript:maker-scaffold495_size155559-snap-gene-0.44-mRNA-1 annotation:"rcc1 and btb domain-containing protein 1")

HSP 1 Score: 95.9005 bits (237), Expect = 6.381e-20
Identity = 64/226 (28.32%), Postives = 108/226 (47.79%), Query Frame = 0

Query:  332 ILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL---HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVP-KWINSISSISNVSTGGFHLI--VILENRSVYSWG 541
            +LA TE  ++YSWG+  + +LG+G     N P ++        +  I+ G HH+  +T  G ++ WG+ + G++G         P  V  L    NV +V+CG   +L +  +G +V+S+G   NG+LG G+      P  +     V++ KV+     +LALS  G L+ WG   +         +S  P K    I  I +++   ++ I   I ++  VY WG
Sbjct:   86 VLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNG-EVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMWG 310          

HSP 2 Score: 89.3521 bits (220), Expect = 6.770e-18
Identity = 63/192 (32.81%), Postives = 97/192 (50.52%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRP-KIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSL-RGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSL-FLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLG 2818
            ++SWG     +LG+G+T +   P ++  +L     T +A G  HS  LTD G+IF +G    G++G G    + SP ++S+L  G  V  VACG                    +T SL  L +G+V+  G +    + +  +N+N  +P K+  L+N+V   +  G  H LALS  G ++GWG N  GQLG
Sbjct:   95 LYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACG--------------------QTSSLALLDNGEVYSWGYNGNGQLGL-GNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLG 265          

HSP 3 Score: 79.337 bits (194), Expect = 1.057e-14
Identity = 79/310 (25.48%), Postives = 134/310 (43.23%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHH-HAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN---VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLIN-FFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG-------LTSLKVKNPKRLDIY--DIDGICAGPTQS---YAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFG 614
            + +T   +VYS G      LG GD      PK++  L    +   + G   H  A T  G LY+WG     +LG+       VP  V+       V  ++CGA H+L ++  G +++++G    G++G G T    +P+ ++  F G  +  V      SLAL  +G+++ WG           N +   P  + ++ +  IS V  G  H + + +  +++ WG NTYG+ G G        T +  +  + +DI     + I A  TQ    Y W      G  H     +P   +F  + ++L  +G
Sbjct:   34 IMVTNSDQVYSLGCNGSSCLGLGDTCSTIVPKKLEQLCNKGVKSFAYGTGPHVLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSG-EIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMW------GQCHGQSVRDPTLTSFRSLHEVLARYG 336          

HSP 4 Score: 68.5514 bits (166), Expect = 1.915e-11
Identity = 43/166 (25.90%), Postives = 75/166 (45.18%), Query Frame = 0

Query: 2577 SSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV----------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            + + CG   +  L  +G ++A G+ + G++G G + +  S   V TL    N++ V                +SWG   +G+LG GN      P  +++L     + V  G +H+  L+D+G +F +G    G+LG GN      P ++++  G  V   A    H
Sbjct:  130 TDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNH 295          

HSP 5 Score: 67.3958 bits (163), Expect = 4.726e-11
Identity = 70/263 (26.62%), Postives = 109/263 (41.44%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTS-HSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQI-SSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL---ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPP-----MPTNFNNSSPTKPRHNIGR 2875
            V+S G      LG G+T     PK +  L        A GT  H    T+ G+++++G+    +LG+G+      P Q+  +L+   VT +ACG  H+            +CL         SG++F  G +   G   + +  N  SP+K+  L   +N++ +A G    LAL  +G V+ WG N  GQLG  +      P  +  L    I ++  G  H+ A         W +            N+  PTK    IGR
Sbjct:   42 VYSLGCNGSSCLGLGDTCSTIVPKKLEQLCNKGVKSFAYGTGPHVLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHS------------LCL-------TDSGEIFAWGQNT-CGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGR 284          

HSP 6 Score: 66.6254 bits (161), Expect = 7.328e-11
Identity = 60/244 (24.59%), Postives = 104/244 (42.62%), Query Frame = 0

Query: 2587 FALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLL-----------LSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSL-SGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIP-KLENMVDIAVGG-DHV-LALSKSGFVWGWG 2810
             A   +G LY+ G   Y +LG G+++       V   L             H+L       +F+WG    G++G G T     P+ +S+L +G     VA G + S  L D G+++++G    G+LG GN   + +P ++++L+   ++ V CG  HT                    L L+     F   +   G     +  N   P K+  ++  +VDIA    +H+  A+++   V+ WG
Sbjct:   87 LACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHT--------------------LALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMWG 310          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold372_size192401-snap-gene-0.50 (protein:Tk00582 transcript:maker-scaffold372_size192401-snap-gene-0.50-mRNA-1 annotation:"rcc1 and btb domain-containing protein 1")

HSP 1 Score: 95.9005 bits (237), Expect = 6.381e-20
Identity = 64/226 (28.32%), Postives = 108/226 (47.79%), Query Frame = 0

Query:  332 ILALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL---PPIMCISAGHHHAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNL---HNVGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLINFFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPWN-------TSSTVP-KWINSISSISNVSTGGFHLI--VILENRSVYSWG 541
            +LA TE  ++YSWG+  + +LG+G     N P ++        +  I+ G HH+  +T  G ++ WG+ + G++G         P  V  L    NV +V+CG   +L +  +G +V+S+G   NG+LG G+      P  +     V++ KV+     +LALS  G L+ WG   +         +S  P K    I  I +++   ++ I   I ++  VY WG
Sbjct:   86 VLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNG-EVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMWG 310          

HSP 2 Score: 89.3521 bits (220), Expect = 6.770e-18
Identity = 63/192 (32.81%), Postives = 97/192 (50.52%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRP-KIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSL-RGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSL-FLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKLENMV--DIAVGGDHVLALSKSGFVWGWGCNFLGQLG 2818
            ++SWG     +LG+G+T +   P ++  +L     T +A G  HS  LTD G+IF +G    G++G G    + SP ++S+L  G  V  VACG                    +T SL  L +G+V+  G +    + +  +N+N  +P K+  L+N+V   +  G  H LALS  G ++GWG N  GQLG
Sbjct:   95 LYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACG--------------------QTSSLALLDNGEVYSWGYNGNGQLGL-GNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLG 265          

HSP 3 Score: 79.337 bits (194), Expect = 1.057e-14
Identity = 79/310 (25.48%), Postives = 134/310 (43.23%), Query Frame = 0

Query:  333 LALTEDHKVYSWGNGSHGRLGHGDKKDYNFPKEISSL--PPIMCISAGHH-HAAAITLDGYLYTWGEGSLGRLGHKDGIVHFVPTLVKNLHN---VGSVSCGAQHTLIVSKDGMQVWSFGCGNNGELGQGDTGCLYTPKLIN-FFDGVIMKKVIASNRFSLALSVSGDLWIWGKTPW-------NTSSTVPKWINSISS--ISNVSTGGFHLIVILENRSVYSWGLNTYGKCGLG-------LTSLKVKNPKRLDIY--DIDGICAGPTQS---YAWTMRKGKGCFHWPYSNEPDAKTFSQVSDILYNFG 614
            + +T   +VYS G      LG GD      PK++  L    +   + G   H  A T  G LY+WG     +LG+       VP  V+       V  ++CGA H+L ++  G +++++G    G++G G T    +P+ ++  F G  +  V      SLAL  +G+++ WG           N +   P  + ++ +  IS V  G  H + + +  +++ WG NTYG+ G G        T +  +  + +DI     + I A  TQ    Y W      G  H     +P   +F  + ++L  +G
Sbjct:   34 IMVTNSDQVYSLGCNGSSCLGLGDTCSTIVPKKLEQLCNKGVKSFAYGTGPHVLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSG-EIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMW------GQCHGQSVRDPTLTSFRSLHEVLARYG 336          

HSP 4 Score: 68.5514 bits (166), Expect = 1.915e-11
Identity = 43/166 (25.90%), Postives = 75/166 (45.18%), Query Frame = 0

Query: 2577 SSVICGLQVTFALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLLLSHNLVXV----------------FSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEH 2726
            + + CG   +  L  +G ++A G+ + G++G G + +  S   V TL    N++ V                +SWG   +G+LG GN      P  +++L     + V  G +H+  L+D+G +F +G    G+LG GN      P ++++  G  V   A    H
Sbjct:  130 TDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNH 295          

HSP 5 Score: 67.3958 bits (163), Expect = 4.726e-11
Identity = 70/263 (26.62%), Postives = 109/263 (41.44%), Query Frame = 0

Query: 2632 VFSWGDGEDGKLGHGNTERCRRPKIISSLSGNFATFVAVGTS-HSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQI-SSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIPKL---ENMVDIAVGGDHVLALSKSGFVWGWGCNFLGQLGNESGYFYKEPIIIEELSGKGILQISAGKNHSAA---------WTSPP-----MPTNFNNSSPTKPRHNIGR 2875
            V+S G      LG G+T     PK +  L        A GT  H    T+ G+++++G+    +LG+G+      P Q+  +L+   VT +ACG  H+            +CL         SG++F  G +   G   + +  N  SP+K+  L   +N++ +A G    LAL  +G V+ WG N  GQLG  +      P  +  L    I ++  G  H+ A         W +            N+  PTK    IGR
Sbjct:   42 VYSLGCNGSSCLGLGDTCSTIVPKKLEQLCNKGVKSFAYGTGPHVLACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHS------------LCL-------TDSGEIFAWGQNT-CGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHTLALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGR 284          

HSP 6 Score: 66.6254 bits (161), Expect = 7.328e-11
Identity = 60/244 (24.59%), Postives = 104/244 (42.62%), Query Frame = 0

Query: 2587 FALQPNGSLYACGEGSYGKLGLGNSDDYFSLTPVVTLL-----------LSHNLV-----XVFSWGDGEDGKLGHGNTERCRRPKIISSL-SGNFATFVAVGTSHSAVLTDKGKIFTFGNGDRGRLGHGNFQTKKSPEQISSLRGYFVTYVACGLEHTKINFAWRESVTKICLGRTFSLFLTSGDVFFCGNSKFSGMDINSSNMNIQSPKKIP-KLENMVDIAVGG-DHV-LALSKSGFVWGWG 2810
             A   +G LY+ G   Y +LG G+++       V   L             H+L       +F+WG    G++G G T     P+ +S+L +G     VA G + S  L D G+++++G    G+LG GN   + +P ++++L+   ++ V CG  HT                    L L+     F   +   G     +  N   P K+  ++  +VDIA    +H+  A+++   V+ WG
Sbjct:   87 LACTESGELYSWGHNGYCQLGNGSTNQGNVPAQVQGALKDKIVTDIACGAHHSLCLTDSGEIFAWGQNTCGQIGSGTTTNQSSPRKVSTLFAGKNVMAVACGQTSSLALLDNGEVYSWGYNGNGQLGLGNNINQLNPSKVTALQNVVISKVVCGYAHT--------------------LALSDEGTLFGWGANTYGQLGTGNKANSCCPTKVATEIGRIVDIAASHYNHISAAITQDSKVYMWG 310          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold22_size673200-snap-gene-2.20 (protein:Tk12673 transcript:maker-scaffold22_size673200-snap-gene-2.20-mRNA-1 annotation:"e3 ubiquitin-protein ligase hectd2")

HSP 1 Score: 92.0485 bits (227), Expect = 1.935e-18
Identity = 71/284 (25.00%), Postives = 130/284 (45.77%), Query Frame = 0

Query: 3074 DMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKT---------------TWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGR--EVPLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVL-AVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMD 3339
            ++R +  +GVL G+A+     + ++  P+ ++ +L                   T  DL D+    ++SL  V   +  D  ED +L   + + +   G+     L P  + I ++ EN+ E+V+  L       + +   A   G  ++     L +L  + +E LVCG  +F +  LKK+  Y      +  I+  WE+LE+++ DL+  FL F +G  R+P          +I R++   + LP A TCF Q+ LP Y  K+ ++ +L  AI +     ++
Sbjct:  534 NLREYNLIGVLMGLAVYNSIILDLHFPPMCYRKLLTPPVVPANDGAPVGIVPSLTVSDLRDIMPDVTQSLANVL-EYEGDIAEDLML--TFSVSVEEYGKVFTTDLKPNGDDITVTNENRKEFVQLYLEWLLNSTIRERFNAFYLGFHSVCASNALIMLRPEEVEQLVCGSQTFDVTELKKVTFYDGYKVDDPTIICFWEVLEDYSEDLQKKFLRFTTGSDRVPVGGTG-DMTIKITRMKDRNHHLPIAHTCFNQIGLPDYKDKDRLKRKLTIAISNAEGFGLE 813          

BLAST of EMLSAG00000002245 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold125_size330479-snap-gene-2.15 (protein:Tk06892 transcript:maker-scaffold125_size330479-snap-gene-2.15-mRNA-1 annotation:"e3 ubiquitin")

HSP 1 Score: 87.8113 bits (216), Expect = 4.928e-17
Identity = 71/281 (25.27%), Postives = 134/281 (47.69%), Query Frame = 0

Query: 3078 FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFS------GR--EVPLYPGSEKIRLSYENKDEYVKAALNQRFKE-MMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYR----DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDV------SQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTIDMD 3339
            F F G L  +AL  +  +        +K +L++  +  DLE +D  + +S+ ++  +   D E          +G++FS      G+  E  L PG + ++++ +NK +Y++  +  R +  +  Q   + KG   +    +L +  ++ +E ++ G +   +   ++   YR    D  P   VI W W  +E F+N+ ++  L FV+G S +P   F         +RF I +  +P + LP A TCF +L LPPY S E +  +L+ A++   T  ++
Sbjct: 1437 FRFCGRLLALALVNKYLLDAFFTRPFYKALLKLPVSLDDLESLDGEFHQSMVWLQENEITDPEA---------LGMTFSVNEEVFGKVIERDLKPGGKHMQVTEKNKKDYIERVVKWRLERGVAAQTDILVKGFYEVVDPRLLAVFDARELELVIAGTAEIDVSDWRRNTEYRSGYYDAHP---VIAWFWMAIERFDNERRLRLLQFVTGTSSVPYEGFSALRGSNGPRRFCIEKWGKP-SSLPRAHTCFNRLDLPPYISPEQLLEKLLLAVEETSTFGIE 1704