RNAi

Search or browse a total of 353 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
1 AX F259 EMLSAG00000012823, EMLSAG00000012823-695589 (gene) Lepeophtheirus salmonis
T6_12823
PreAdult2 normal 30/13 Lepeophtheirus salmonis 5 August, 2019 - 13:45
2 AX F260 EMLSAT00000011145, EMLSAT00000011145-706992 (mRNA) Lepeophtheirus salmonis
T16_11145
PreAdult2 normal 30/21 Lepeophtheirus salmonis 5 August, 2019 - 13:42
3 AX F262 EMLSAG00000006928, EMLSAG00000006928-689694 (gene) Lepeophtheirus salmonis
T19_6928
PreAdult2 normal 30/14 Lepeophtheirus salmonis 5 August, 2019 - 13:39
4 AX F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185
PreAdult2 normal 30/22 Lepeophtheirus salmonis 5 August, 2019 - 10:18
5 AV F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_2
PreAdult2 normal
no visible phenotype
30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:36
6 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2
PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:32
7 AV F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_1
PreAdult2 normal
no visible phenotype
30/11 Lepeophtheirus salmonis 3 May, 2019 - 13:15
8 AV F353 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/11 Lepeophtheirus salmonis 3 May, 2019 - 12:33
9 AV F352 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype
30/11 Lepeophtheirus salmonis 2 May, 2019 - 11:24
10 F0 cop + infection CPY185 (negative control), CPY185 (PCR_product) Gadus morhua Nauplius1, Copepodid No visible phenotype 60/52 Lepeophtheirus salmonis 2 May, 2019 - 11:21

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