RNAi

Search or browse a total of 415 RNA interference (RNAi) experiments available in LiceBase. RNA interference is a technique to "silence" a gene's expression by injection of double-stranded RNA (dsRNA) or short-interfering RNA (siRNA).
At SLRC, dsRNA has been widely used and can be applied at Nauplia and pre-adult and adult stages of the salmon louse. The animals are then compared to control animals for differences in phenotype. Most are only inspected visually, but some also histologically or measurements, e.g. of egg-string length. Note: "No visible phenotype" means that no difference could be observed under the outlined experimental conditions and using the observation methods applied.

Select one or many phenotypes
List of all RNAi experiments
Gallery Image Title Primary target ID Developmental Stage CV Phenotype Number of Individuals (Start/End) Organism Efficacy of Knock Down Postedsort ascending
1 AX F418 EMLSAT00000010925, EMLSAT00000010925-706772 (mRNA) Lepeophtheirus salmonis
Trypsin- Dylan 		PreAdult2 survival, reduced 30/3 Lepeophtheirus salmonis 5 August, 2019 - 13:49
2 AX F259 EMLSAG00000012823, EMLSAG00000012823-695589 (gene) Lepeophtheirus salmonis
T6_12823 		PreAdult2 normal 30/13 Lepeophtheirus salmonis 5 August, 2019 - 13:45
3 AX F260 EMLSAT00000011145, EMLSAT00000011145-706992 (mRNA) Lepeophtheirus salmonis
T16_11145 		PreAdult2 normal 30/21 Lepeophtheirus salmonis 5 August, 2019 - 13:42
4 AX F262 EMLSAG00000006928, EMLSAG00000006928-689694 (gene) Lepeophtheirus salmonis
T19_6928 		PreAdult2 normal 30/14 Lepeophtheirus salmonis 5 August, 2019 - 13:39
5 AX F0 CPY185 (negative control), CPY185 (PCR_product) Gadus morhua
CPY185 		PreAdult2 normal 30/22 Lepeophtheirus salmonis 5 August, 2019 - 10:18
6 AV F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_2 		PreAdult2 normal
no visible phenotype 		30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:36
7 AP F353 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874 Var2_2 		PreAdult2, Adult no visible phenotype 30/14 Lepeophtheirus salmonis 3 May, 2019 - 13:32
8 AV F352 EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis
4874_Var2_1 		PreAdult2 normal
no visible phenotype 		30/11 Lepeophtheirus salmonis 3 May, 2019 - 13:15
9 AV F353 EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis PreAdult2 normal
no visible phenotype 		30/11 Lepeophtheirus salmonis 3 May, 2019 - 12:33
10 AT F394 + F149 EMLSAT00000005702, EMLSAT00000005702-701549 (mRNA) Lepeophtheirus salmonis PreAdult2 No Visible Phenotype 40/13 Lepeophtheirus salmonis F>69.00% 2 May, 2019 - 11:41

Pages