EMLSAG00000003336, EMLSAG00000003336-686102 (gene) Lepeophtheirus salmonis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Long-chain-fatty-acid--CoA ligase ACSBG2" species:9031 "Gallus gallus" [GO:0003824 "catalytic activity" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0003824 InterPro:IPR020845 GeneTree:ENSGT00690000101725 OrthoDB:EOG7B5WVB TreeFam:TF354286 EMBL:AADN03009059 EMBL:AADN03009019 Ensembl:ENSGALT00000002682 Uniprot:F1NI43) HSP 1 Score: 474.552 bits (1220), Expect = 3.748e-147 Identity = 276/673 (41.01%), Postives = 384/673 (57.06%), Query Frame = 0
Query: 10 TDKMFPNKQALASSYT------TTKPDKGVHIRTESSEFLSFSVPNMV----KNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK---HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQKVI--HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSV--LITEKIEDAKEYTTT 645
T++ NK LASS TT+ D V +R + E + P V + G ALA +NG WVK Y YY AK+F+KLGL+ F+ VCI FN PEWFI+++GAIFAGGL+ G+Y TNS Y+ N ILVVEN L+ I I + +K I+ E+ NL W++ L+LG +L + + NQCC+LIYTSGTTG PKGVMLSHD +T+ + A + + T + +VVSYLPLSH+AA +DI++ + G+V+FA ALKG+LVDTL+EVRPT F GVPRVWEK +E +K + KVA K + + NG +V ++ +L+++KV++A+GLDRC + +G AP+S T +FFLSL+IPV E YG+SE G F+ +CG + + K D I GEIC GR + MGYL E+ T EA DK+G+ H TGR+K+L++T+GGENV P PIE+A+K P++SNA++VGD KF+++ LT+K + + E GEP +LT + + LG T V EI+ K V AIQ + E NKRA+SNA K++KW +L ++ S+ GE+G T+ ++ LI E DA TTT
Sbjct: 57 TERSQMNKTGLASSSRPASNVWTTQQDGEVKLRMDE-EGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLGLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQLQKILEIEHKLPHMKAIIQYGEELKEKRPNLYSWREFLDLGEDVPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGI-GEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFYADANTPTTT 727 HSP 2 Score: 394.815 bits (1013), Expect = 4.929e-118 Identity = 235/652 (36.04%), Postives = 356/652 (54.60%), Query Frame = 0
Query: 620 VNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTA----KTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYG-----------------LSECG---------------------------IFSDFAPSEYKK---------------GTCGSI-FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+N G SSS A TT DG V +R ++ + P +V + K G A+A K+NG W++ TY YY + AAK+F+KLGL+ FH V I FN PEW I+++GAIFAGG A G+Y +NS Y+ N ILVVEN+ LQK+ I + H+ ++ E+ + +K L WR+ ++LG+ + QL++ + + NQCC++IYTSGTTG PKGVM+SHD LT+T+ ++++ K+ +VSYLPLSH+AA +DI++ + G+++FA ALKG+LV TL+EVRPT F GVPRV+EK +E ++ +K ++ + + K N G+ + N L Q++YKK++ ++GLD+C + G AP+ T +FFLSL+I + YG L+ CG IF + E K G C F GR KEL+ITAGGEN+ P+ IED +K P++SN + +GD KF+++ LT K +N+++ G+P +L+ E+C LG +A+ V+ II + V +AIQ + N+ VSNA +++KW ++ ++ S+ GE+GPT+KLKR
Sbjct: 62 MNKTGLASSS-------RPASNVWTTQQDGEVKLRMDEEGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLGLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQLQKILEIEHKLPHMKAIIQYGEE--LKEKRPNLYSWREFLDLGEDVPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGIGEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTES-GEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKR 703 HSP 3 Score: 112.079 bits (279), Expect = 1.205e-23 Identity = 53/134 (39.55%), Postives = 88/134 (65.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFK-VNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IED +K P++SN + +GD KF+++ LT K + + ++G+P +L+ ++C L +A+ V+ II K V +AIQ + + NK AVSNA K++KW ++ ++ S+ GE+GPT+
Sbjct: 566 KELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTM 699
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Long-chain-fatty-acid--CoA ligase ACSBG2" species:9031 "Gallus gallus" [GO:0003824 "catalytic activity" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0003824 InterPro:IPR020845 GeneTree:ENSGT00690000101725 EMBL:AADN03009059 EMBL:AADN03009019 Ensembl:ENSGALT00000045836 Uniprot:R4GFY4) HSP 1 Score: 474.167 bits (1219), Expect = 6.171e-147 Identity = 276/673 (41.01%), Postives = 384/673 (57.06%), Query Frame = 0
Query: 10 TDKMFPNKQALASSYT------TTKPDKGVHIRTESSEFLSFSVPNMV----KNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK---HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQKVI--HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSV--LITEKIEDAKEYTTT 645
T++ NK LASS TT+ D V +R + E + P V + G ALA +NG WVK Y YY AK+F+KLGL+ F+ VCI FN PEWFI+++GAIFAGGL+ G+Y TNS Y+ N ILVVEN L+ I I + +K I+ E+ NL W++ L+LG +L + + NQCC+LIYTSGTTG PKGVMLSHD +T+ + A + + T + +VVSYLPLSH+AA +DI++ + G+V+FA ALKG+LVDTL+EVRPT F GVPRVWEK +E +K + KVA K + + NG +V ++ +L+++KV++A+GLDRC + +G AP+S T +FFLSL+IPV E YG+SE G F+ +CG + + K D I GEIC GR + MGYL E+ T EA DK+G+ H TGR+K+L++T+GGENV P PIE+A+K P++SNA++VGD KF+++ LT+K + + E GEP +LT + + LG T V EI+ K V AIQ + E NKRA+SNA K++KW +L ++ S+ GE+G T+ ++ LI E DA TTT
Sbjct: 69 TERSQMNKTGLASSSRPASNVWTTQQDGEVKLRMDE-EGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLGLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQLQKILEIEHKLPHMKAIIQYGEELKEKRPNLYSWREFLDLGEDVPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGI-GEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFYADANTPTTT 739 HSP 2 Score: 394.815 bits (1013), Expect = 7.336e-118 Identity = 235/652 (36.04%), Postives = 356/652 (54.60%), Query Frame = 0
Query: 620 VNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTA----KTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYG-----------------LSECG---------------------------IFSDFAPSEYKK---------------GTCGSI-FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+N G SSS A TT DG V +R ++ + P +V + K G A+A K+NG W++ TY YY + AAK+F+KLGL+ FH V I FN PEW I+++GAIFAGG A G+Y +NS Y+ N ILVVEN+ LQK+ I + H+ ++ E+ + +K L WR+ ++LG+ + QL++ + + NQCC++IYTSGTTG PKGVM+SHD LT+T+ ++++ K+ +VSYLPLSH+AA +DI++ + G+++FA ALKG+LV TL+EVRPT F GVPRV+EK +E ++ +K ++ + + K N G+ + N L Q++YKK++ ++GLD+C + G AP+ T +FFLSL+I + YG L+ CG IF + E K G C F GR KEL+ITAGGEN+ P+ IED +K P++SN + +GD KF+++ LT K +N+++ G+P +L+ E+C LG +A+ V+ II + V +AIQ + N+ VSNA +++KW ++ ++ S+ GE+GPT+KLKR
Sbjct: 74 MNKTGLASSS-------RPASNVWTTQQDGEVKLRMDEEGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLGLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQLQKILEIEHKLPHMKAIIQYGEE--LKEKRPNLYSWREFLDLGEDVPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGIGEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTES-GEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKR 715 HSP 3 Score: 112.464 bits (280), Expect = 1.066e-23 Identity = 53/134 (39.55%), Postives = 88/134 (65.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFK-VNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IED +K P++SN + +GD KF+++ LT K + + ++G+P +L+ ++C L +A+ V+ II K V +AIQ + + NK AVSNA K++KW ++ ++ S+ GE+GPT+
Sbjct: 578 KELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTM 711
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:acsbg2 "acyl-CoA synthetase bubblegum family member 2" species:7955 "Danio rerio" [GO:0003824 "catalytic activity" evidence=IEA] [GO:0008152 "metabolic process" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 ZFIN:ZDB-GENE-030131-7099 GO:GO:0003824 InterPro:IPR020845 GeneTree:ENSGT00690000101725 HOGENOM:HOG000230004 HOVERGEN:HBG054660 OrthoDB:EOG7B5WVB TreeFam:TF354286 OMA:QRANQCA EMBL:BX664738 ProteinModelPortal:B0S7E7 Ensembl:ENSDART00000124809 PRO:PR:B0S7E7 ArrayExpress:B0S7E7 Uniprot:B0S7E7) HSP 1 Score: 471.47 bits (1212), Expect = 1.383e-145 Identity = 263/653 (40.28%), Postives = 370/653 (56.66%), Query Frame = 0
Query: 2 EAEHSNSMTDKMFPNKQALA-----SSYTTTKPDKGVHIRTESSEF---LSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAI---VKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDAS-------MHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+AE S T P +L+ + TT D V +R S +V M + GS AL W Y +YY + AK+F+KLGL+ ++ V I FN EWFI+++ AI AGG + G+Y TNS +Y+ N + ILVVEN + L+ I I + LK I+ +++ NL W + +ELGR E +L+D +S NQCC+LIYTSGTTG PKGVMLSHD +T+ A V +D + +VVSYLPLSHIAA +DI++ + G YFA ALKGSL +TL+E+RPT F GVPRVWEK QE +K + KVA KD M N + + +++ L+F+KV++ALGLDRC +G AP+++ T +FFLSLDIP+ E YG+SE G Q + F+ +CG +IP K + + DD+ GEIC GR V MGYL T EA D EG+ H TGR+K+L++T+GGEN+ P PIE+A+K +P++SNA+++GD KF+S+ LT+K + N E G P ELT + + LG + T V EI + V AIQ GI+ N++A SNA +++KW +L ++ S+ GE+G T+
Sbjct: 108 KAEQSTHTTSGATPRPDSLSLPTKGTELWTTHRDGEVKLRMAESGLGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQWKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPHLKAIIQYKDELKEKKPNLYTWAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIPGCKTK-LSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTM 759 HSP 2 Score: 365.155 bits (936), Expect = 4.533e-107 Identity = 228/639 (35.68%), Postives = 333/639 (52.11%), Query Frame = 0
Query: 641 EYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSI-LWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK----KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN-------TTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQ-----------------GR-----------KKELLITAGG-----------EN--------IAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
E TT DG V +R + L P++V + GS A+ K W TYS+YY+ R AAK+F+KLGL+ +H V I FN EW I+++ AI AGGFA G+Y +NS +Y+ N + ILVVEN+ LQK+ I + + H LK ++ +D K ++ W + +ELG+ E + QL + + NQCC++IYTSGTTG PKGVM+SHD LT+TA + + +VSYLPLSHIAA +DI++ + G YFA ALKGSL TL+E+RPT F GVPRV+EK QE ++ +K ++ + + KD N + K L ++++K++ +LGLD+C G AP+ T +FFLSLDI + YG+SE G + + ++ +CG + P + GR K E + A G EN I ++I +N PV +SN + IGD +KF+S+ LT K +N +T G P EL+ E+C LG + VT I + V +AIQ GI+ NE SNA R++KW+++ ++ S+ GE+GPT+KLKR K++
Sbjct: 134 ELWTTHRDGEVKLRMAESGLGAETPLTVNQMFTSTVQRFGSYPALGWKEGEQWKTMTYSEYYKSCRTAAKSFLKLGLERYHGVGILGFNSTEWFIADIAAILAGGFAVGIYTTNSPEACQYVAENCQANILVVENHKQLQKILQIQDKLPH-LKAIIQYKDELKEKKPNLYTWAEFMELGRDEPDTQLDDIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAVATSQHVSLNDADKLQEVVVSYLPLSHIAAQMIDIWLPMKAGGVTYFAQPDALKGSLANTLREIRPTAFLGVPRVWEKMQEKMKSIGAKSSTVRRKVASWAKDVGLQTNLNKMELGSNSRKPLNYRLAKHLVFRKVRKALGLDRCTRCYTGAAPITKDTLEFFLSLDIPLFELYGMSESSGPHTITQANAFRLTSCGKVIPGCKTKLSNPDDEKNGEICFWGRHVFMGYLNMPDKTEEALDAEGWLHSGDLGKLDENNFLFITGRIKELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGET-GVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTMKLKRPVVMKMY 770 HSP 3 Score: 110.538 bits (275), Expect = 3.909e-23 Identity = 54/134 (40.30%), Postives = 86/134 (64.18%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVN-SDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ IED +K +P++SN + IGD +KF+S+ LT K + + G P EL+ ++C L + VT I + V +AIQ GI++ N+ A SNA +++KW+++ ++ S+ GE+GPT+
Sbjct: 626 KELIITAGGENIPPVPIEDAVKEAIPLISNAMLIGDKRKFLSMLLTVKCQINGETGVPEDELTPEAVEFCRKLGSSCTRVTEIAGGRDRAVQAAIQDGINRVNEKATSNAQRIQKWTVLDQDFSIPGGELGPTM 759
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Long-chain-fatty-acid--CoA ligase ACSBG2" species:9031 "Gallus gallus" [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IEA] [GO:0005524 "ATP binding" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0005524 GO:GO:0005737 InterPro:IPR020845 GO:GO:0004467 eggNOG:COG1022 HOGENOM:HOG000230004 HOVERGEN:HBG054660 KO:K15013 EMBL:AJ720017 RefSeq:NP_001012864.1 UniGene:Gga.22498 ProteinModelPortal:Q5ZKR7 STRING:9031.ENSGALP00000002679 PaxDb:Q5ZKR7 GeneID:420090 KEGG:gga:420090 CTD:81616 InParanoid:Q5ZKR7 NextBio:20823052 PRO:PR:Q5ZKR7 GO:GO:0001676 Uniprot:Q5ZKR7) HSP 1 Score: 464.151 bits (1193), Expect = 2.504e-143 Identity = 276/682 (40.47%), Postives = 384/682 (56.30%), Query Frame = 0
Query: 10 TDKMFPNKQALASSYT------TTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKT----ALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVEN-LQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK---HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQKVI--HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSV--LITEKIEDAKEYTTTLPDGSVII 653
T++ NK LASS TT+ D V +R + E + P V SK ALA +NG WVK Y YY AK+F+KL L+ F+ VCI FN PEWFI+++GAIFAGGL+ G+Y TNS Y+ N ILVVEN Q R I + +K I+ E+ N W++ L+LG +L + + NQCC+LIYTSGTTG PKGVMLSHD +T+ + A + + T + +VVSYLPLSH+AA +DI++ + G+V+FA ALKG+LVDTL+EVRPT F GVPRVWEK +E +K + KVA K + + NG +V ++ +L+++KV++A+GLDRC + +G AP+S T +FFLSL+IPV E YG+SE G F+ +CG + + K D I GEIC GR + MGYL E+ T EA DK+G+ H TGR+K+L++T+GGENV P PIE+A+K P++SNA++VGD KF+++ LT+K + + E GEP +LT + + LG T V EI+ K V AIQ + E NKRA+SNA K++KW +L ++ S+ GE+G T+ ++ LI E DA TTT S ++
Sbjct: 57 TERSQMNKTGLASSSRPASNVWTTQQDGEVKLRMDE-EGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQACRKSLEIEHKLPHMKAIIQYGEELKEKRPNQYSWREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGI-GEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFYADANTPTTTEERTSAVM 736 HSP 2 Score: 380.948 bits (977), Expect = 5.013e-113 Identity = 230/651 (35.33%), Postives = 349/651 (53.61%), Query Frame = 0
Query: 620 VNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNX-VLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTA----KTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYG-----------------LSECG---------------------------IFSDFAPSEYKK---------------GTCGSI-FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+N G SSS A TT DG V +R ++ + P +V + K G A+A K+NG W++ TY YY + AAK+F+KL L+ FH V I FN PEW I+++GAIFAGG A G+Y +NS Y+ N ILVVEN+ +K I + H+ ++ E+ WR+ ++LG+ + QL++ + + NQCC++IYTSGTTG PKGVM+SHD LT+T+ ++++ K+ +VSYLPLSH+AA +DI++ + G+++FA ALKG+LV TL+EVRPT F GVPRV+EK +E ++ +K ++ + + K N G+ + N L Q++YKK++ ++GLD+C + G AP+ T +FFLSL+I + YG L+ CG IF + E K G C F GR KEL+ITAGGEN+ P+ IED +K P++SN + +GD KF+++ LT K +N+++ G+P +L+ E+C LG +A+ V+ II + V +AIQ + N+ VSNA +++KW ++ ++ S+ GE+GPT+KLKR
Sbjct: 62 MNKTGLASSS-------RPASNVWTTQQDGEVKLRMDEEGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQACRKSLEIEHKLPHMKAIIQYGEELKEKRPNQYSWREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGIGEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTES-GEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKR 704 HSP 3 Score: 112.079 bits (279), Expect = 1.231e-23 Identity = 53/134 (39.55%), Postives = 88/134 (65.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFK-VNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IED +K P++SN + +GD KF+++ LT K + + ++G+P +L+ ++C L +A+ V+ II K V +AIQ + + NK AVSNA K++KW ++ ++ S+ GE+GPT+
Sbjct: 567 KELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTM 700
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0005829 "cytosol" evidence=IEA] [GO:0047617 "acyl-CoA hydrolase activity" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0005829 GO:GO:0005739 InterPro:IPR020845 GO:GO:0004467 GeneTree:ENSGT00690000101725 KO:K15013 OrthoDB:EOG7B5WVB TreeFam:TF354286 CTD:81616 GO:GO:0047617 OMA:EHIVCID EMBL:DAAA02019570 RefSeq:XP_001790634.1 RefSeq:XP_002688930.1 RefSeq:XP_005198994.1 RefSeq:XP_005208962.1 UniGene:Bt.61542 ProteinModelPortal:E1BCN4 Ensembl:ENSBTAT00000012001 GeneID:526688 KEGG:bta:526688 NextBio:20874424 Uniprot:E1BCN4) HSP 1 Score: 460.299 bits (1183), Expect = 6.325e-143 Identity = 254/606 (41.91%), Postives = 362/606 (59.74%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVE-NLQVLRTISHILKNVTTLKKIVVIEEDGFRPG------ENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTA---KFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQM-----FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
++P + GS ALA +NG W NY+QYY + TA+A +KLGL+PF++V I FN EW ++ LGAIFAGG G+Y TN + EY++ +++ IL++E N Q+ + +S + + TLK IV ++P ENL W D LELG +L+ + NQC +IYTSGTTG PKGVMLSHD IT+ ++ K+ + A + +VVSYLPLSHIAA +D++V + + +YFA+ ALKG+LV+TL+EV+PT F GVPR+WEK E IKE + KV K S D + +++ L+F K+K +LGLD C FISG APL++ T +FFLSLDIP+ E YG+SE G +++K + G ++ + + + D + GE+C+ GR V MGYL E AT EA D EG+ H TGR+K++++T+GGENVAP PIEN +K +P++SNA++VGD KF+S+ LT+K + ++ GEP L K + +G TTV EIL + P V AIQKGI+ N++AISNA K++KW IL ++ S+ GE+G T
Sbjct: 58 LTIPEFFRESVNRFGSYPALASKKNGRWEVLNYNQYYEACRKTARAMLKLGLEPFHSVGILGFNSIEWILAALGAIFAGGFCVGIYATNFADACEYVITHAKVNILLLENNAQLQKILSIPMNKMETLKAIV-----QYKPPVDDSKCENLYSWDDFLELGSSIPDAQLDQIIKSQKANQCAVIIYTSGTTGQPKGVMLSHDNITWTAGSVAKECNLAYAPEKQEVVVSYLPLSHIAAQMMDVWVPIKVGAFIYFAEPDALKGTLVNTLQEVKPTAFLGVPRIWEKMHEKIKEAVGKSSSLRKKVFLWARNIGLKVNSKRMLGEHDHPMSYRMAKALVFTKIKNSLGLDHCRIFISGAAPLNQETSEFFLSLDIPIGEVYGMSESSGPHTISTPANYKFLSSGKVVAGCKNMLYQPNKDGV-GEVCLWGRHVFMGYLEQEDATMEAIDDEGWLHSGDLGRMDNQGFLFITGRIKEIIITAGGENVAPVPIENLVKETIPIISNAMLVGDKAKFLSILLTLKCEVDKMTGEPLDTLNLEAIKFCREVGSQATTVSEILDLRDPMVYAAIQKGINAVNQKAISNAQKIQKWVILEKDFSISGGELGPT 657 HSP 2 Score: 313.538 bits (802), Expect = 1.871e-90 Identity = 174/418 (41.63%), Postives = 249/418 (59.57%), Query Frame = 0
Query: 648 DGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEK----HGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNT-----TGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSI 1050
DG V++R K P+++P F + + + GS A+A K+NG W Y+QYY R A+A +KLGL+PFH+V I FN EW+++ LGAIFAGGF G+Y +N A EY++ +++ IL++ENN LQK+ I N LK +V + K L W D +ELG + + QL Q + + ANQC IIYTSGTTG PKGVM+SHD +T+TA +V ++ + K+ +VSYLPLSHIAA +D++V I +YFA+ ALKG+LV TL+EV+PT F GVPR++EK E I+E + K +K + ++ N+ + + +++ KIK+SLGLD C+ F G APL T++FFLSLDI I YG+SE G + P+ YK + G +
Sbjct: 40 DGEVLMRLSKHGPGHESPLTIPEFFRESVNRFGSYPALASKKNGRWEVLNYNQYYEACRKTARAMLKLGLEPFHSVGILGFNSIEWILAALGAIFAGGFCVGIYATNFADACEYVITHAKVNILLLENNAQLQKILSIPMNKMETLKAIVQYKPPVDDSKCENLYSWDDFLELGSSIPDAQLDQIIKSQKANQCAVIIYTSGTTGQPKGVMLSHDNITWTAGSVAKECNLAYAPEKQEVVVSYLPLSHIAAQMMDVWVPIKVGAFIYFAEPDALKGTLVNTLQEVKPTAFLGVPRIWEKMHEKIKEAVGKSSSLRKKVFLWARNIGLKVNSKRMLGEHDHPMSYRMAKALVFTKIKNSLGLDHCRIFISGAAPLNQETSEFFLSLDIPIGEVYGMSESSGPHTISTPANYKFLSSGKV 457 HSP 3 Score: 132.109 bits (331), Expect = 5.011e-30 Identity = 65/155 (41.94%), Postives = 103/155 (66.45%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNE-FSKIHFNIYSF 1208
GR KE++ITAGGEN+AP+ IE+ +K +P++SN + +GD KF+S+ LT K D G+P L+ ++C +G +A+TV+ I+ + P V +AIQKGI+ N+ +SNA +++KW ++ ++ S+ GE+GPT K+KR+ K IYSF
Sbjct: 522 GRIKEIIITAGGENVAPVPIENLVKETIPIISNAMLVGDKAKFLSILLTLKCEVDKMTGEPLDTLNLEAIKFCREVGSQATTVSEILDLRDPMVYAAIQKGINAVNQKAISNAQKIQKWVILEKDFSISGGELGPTTKIKRHFILQKYKRQIYSF 676 HSP 4 Score: 111.309 bits (277), Expect = 1.942e-23 Identity = 54/133 (40.60%), Postives = 88/133 (66.17%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG EN+AP+ IE+ +K +P++SN + +GD KF+S+ LT K DK G+P L+ +C + +A+TV+ I+ + V +AIQKGI+ N+ A+SNA K++KW ++ ++ S+ GE+GPT
Sbjct: 525 KEIIITAGGENVAPVPIENLVKETIPIISNAMLVGDKAKFLSILLTLKCEVDKMTGEPLDTLNLEAIKFCREVGSQATTVSEILDLRDPMVYAAIQKGINAVNQKAISNAQKIQKWVILEKDFSISGGELGPT 657
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0005829 "cytosol" evidence=IEA] [GO:0047617 "acyl-CoA hydrolase activity" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0005829 GO:GO:0005739 InterPro:IPR020845 GO:GO:0004467 GeneTree:ENSGT00690000101725 OrthoDB:EOG7B5WVB TreeFam:TF354286 OMA:QRANQCA GO:GO:0047617 EMBL:FP085490 Ensembl:ENSSSCT00000014783 Uniprot:F1SBT8) HSP 1 Score: 459.914 bits (1182), Expect = 7.490e-143 Identity = 261/650 (40.15%), Postives = 383/650 (58.92%), Query Frame = 0
Query: 2 EAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIRTES---SEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKN-VTTLKKI----VVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTA---KFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKE---QSKNQKKSQMKVAQM--FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
+ E + + M +++ + TT D V +R ++P + G+ ALA +N W N++QYY + KA +KLGL+PF++V I FN EW I+ LGAI AGG G+Y TNS+ +Y++ +++ IL+VEN L+ I I K+ + TLK I V ++E NL W D +ELGR +L+ + + NQC +IYTSGT GNPKGVMLSHD IT+M A ++++ A + +VVSYLPLSHIAA +D+++ + + +YFA ALKG+LV+TL+EV+PT F GVPR+WEK E IKE +S + +K A+ FK + D + +++ L+F KVK ++GLD C FISG APL++ T +FFLSLDI + E YG++E G ++K + G +IP + + + D + GEIC+ GR + MGYL E AT EA D+EG+ H TGR+K++++T+GGENVAP PIEN +K +P+VSNA++VGD KF+S+ LT+K + ++ GEP +LT K +++G +TV EIL + P V AIQKGI+ N++AISNA K++KW IL ++ S+ GE+G T
Sbjct: 8 QEEKAKDLKVDMSQSQEQVTPGLWTTHRDGEVLLRLSKHGPGHETPMTIPEFFRESVSRFGTYPALASKKNEQWEVLNFNQYYEACRKAGKAMMKLGLEPFHSVGILGFNSVEWLIAALGAILAGGFCVGIYATNSTDACQYVITHAKVNILIVENDMQLQKILAIPKSRMETLKAIIQYKVPVKES---KSNNLYSWDDFMELGRSIPDAQLDQIIRNQKANQCAVIIYTSGTIGNPKGVMLSHDNITWMAGAAAREYNLAYACERQEVVVSYLPLSHIAAQMMDVWIPMKVGAFIYFAQPNALKGTLVNTLQEVKPTAFLGVPRIWEKMHEKIKEAIDKSSSLRKKVFSWARTTGFKVNTKRMMGGHDTPMSYRMAKALVFSKVKSSVGLDHCHIFISGAAPLNQETAEFFLSLDIRIGEAYGMTESSGPHTLSNHENYKILSSGKVIPGCKNMLYQQTKDGV-GEICLWGRHIFMGYLEREDATMEAIDEEGWLHSGDLGRMDNQGFLFITGRIKEIIITAGGENVAPIPIENLVKEKIPIVSNAMLVGDKAKFLSILLTLKCEFDKLTGEPLDKLTWEAIKFCRDMGSQASTVTEILELQDPLVYTAIQKGINAVNQQAISNAQKIQKWAILEKDFSISGGELGPT 653 HSP 2 Score: 373.629 bits (958), Expect = 2.350e-111 Identity = 224/628 (35.67%), Postives = 338/628 (53.82%), Query Frame = 0
Query: 644 TTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT----KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT-----GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSE-YKKGTCGSIFPSTQ-----------------GR--------KKELLITAGGE----------------------NIAPILIEDNIKNFLPV-LSNVVA-----------IGDLKKFISVFLTFKVNSDT-NGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
TT DG V++R K PM++P F + + + G+ A+A K+N W ++QYY R A KA +KLGL+PFH+V I FN EW+I+ LGAI AGGF G+Y +NS +Y++ +++ IL+VEN+ LQK+ I ++ LK ++ + K + L W D +ELG++ + QL Q + N ANQC IIYTSGT GNPKGVM+SHD +T+ A ++ ++ +VSYLPLSHIAA +D+++ + +YFA +ALKG+LV TL+EV+PT F GVPR++EK E I+E I K +K + + + F NT + + +++ K+K S+GLD C F G APL T +FFLSLDI I AYG++E + E YK + G + P + GR +++ + A E I I+I +N P+ + N+V +GD KF+S+ LT K D G+P +L+ ++C +G +ASTVT I+ Q P V +AIQKGI+ N+ +SNA +++KW+++ ++ S+ GE+GPT K+KR+
Sbjct: 32 TTHRDGEVLLRLSKHGPGHETPMTIPEFFRESVSRFGTYPALASKKNEQWEVLNFNQYYEACRKAGKAMMKLGLEPFHSVGILGFNSVEWLIAALGAILAGGFCVGIYATNSTDACQYVITHAKVNILIVENDMQLQKILAIPKSRMETLKAIIQYKVPVKESKSNNLYSWDDFMELGRSIPDAQLDQIIRNQKANQCAVIIYTSGTIGNPKGVMLSHDNITWMAGAAAREYNLAYACERQEVVVSYLPLSHIAAQMMDVWIPMKVGAFIYFAQPNALKGTLVNTLQEVKPTAFLGVPRIWEKMHEKIKEAIDKSSSLRKKVFSWARTTGFKVNTKRMMGGHDTPMSYRMAKALVFSKVKSSVGLDHCHIFISGAAPLNQETAEFFLSLDIRIGEAYGMTESSGPHTLSNHENYKILSSGKVIPGCKNMLYQQTKDGVGEICLWGRHIFMGYLEREDATMEAIDEEGWLHSGDLGRMDNQGFLFITGRIKEIIITAGGENVAPIPIENLVKEKIPIVSNAMLVGDKAKFLSILLTLKCEFDKLTGEPLDKLTWEAIKFCRDMGSQASTVTEILELQDPLVYTAIQKGINAVNQQAISNAQKIQKWAILEKDFSISGGELGPTTKIKRH 659 HSP 3 Score: 116.316 bits (290), Expect = 5.827e-25 Identity = 57/133 (42.86%), Postives = 90/133 (67.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG EN+API IE+ +K +P++SN + +GD KF+S+ LT K DK G+P +L+ +C + +ASTVT I+ + V +AIQKGI+ N+ A+SNA K++KW+++ ++ S+ GE+GPT
Sbjct: 521 KEIIITAGGENVAPIPIENLVKEKIPIVSNAMLVGDKAKFLSILLTLKCEFDKLTGEPLDKLTWEAIKFCRDMGSQASTVTEILELQDPLVYTAIQKGINAVNQQAISNAQKIQKWAILEKDFSISGGELGPT 653
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0003824 "catalytic activity" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 GO:GO:0003824 GeneTree:ENSGT00690000101725 OrthoDB:EOG7B5WVB OMA:QRANQCA EMBL:AAEX03012500 Ensembl:ENSCAFT00000029716 Uniprot:F1PS77) HSP 1 Score: 458.759 bits (1179), Expect = 1.936e-142 Identity = 250/600 (41.67%), Postives = 358/600 (59.67%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVEN-LQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTA---KFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQM-----FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
++P + G ALA + +W N++QYY + A+A IKLGL F+ V I FN EW I++LGAI AGGL G+Y TNS+ +Y++ N++ +L+VEN LQ+ + +S + TLK I+ + NL W D +ELG +L+ + NQC +IYTSGT GNPKGVMLSHD IT+ A+ K + A + +VVSYLPLSH+AA +D+++ + + +YFA AL G+L++TL EV+PT F GVPR+WEK QE IKE + KV K + + D + +++ L+F KV+ ALGLD+C ISG APL+ T +FFLSLDIP+ E YG+SE G + ++++K +CG I+ + + S D GEICI GR V MGYL E AT EA D+EG+ H TGR+K++L+T+GGENVAP PIEN +K +P++SNA++VGD KF+S+ LT+K + + +GEP +L+ + + LG + +TV EIL + P V KAIQ+GID N+ AISNA +++KW IL ++ S+ GE+G T
Sbjct: 52 LTIPEFFRESVSRFGMYPALATKNSEHWEVLNFNQYYEACRKAARALIKLGLQRFHGVGILGFNSVEWLIASLGAILAGGLCVGIYATNSADACQYVITNAKVNVLLVENDLQLQKILSIPQSRMETLKAIIQYKLPVKESNNNLYSWNDFMELGNSIPDSQLDQIIESQRANQCAVIIYTSGTLGNPKGVMLSHDNITWTAGAVAKNCSLSNAAEKQEVVVSYLPLSHVAAQMMDVWIPMKIGAFIYFAQPDALTGTLINTLLEVKPTAFLGVPRIWEKMQEKIKESGAKCSSLRKKVFSWGRIIGLKVNTKRMFGVHDTTMSYRVAKALVFSKVRNALGLDQCQIPISGAAPLNPETSEFFLSLDIPIGEMYGMSESTGPHTTSSRNNYKIHSCGKIMSGCKNMLYQQSKDGT-GEICIWGRHVFMGYLEMEDATMEAIDEEGWLHTGDLGRVDSHGFLYITGRIKEILITAGGENVAPVPIENMVKEKIPIISNAILVGDKAKFLSILLTLKCEVDRRNGEPLDKLSLEAIQFCRKLGSHVSTVSEILELQDPLVYKAIQQGIDAVNQEAISNAQRIQKWVILEKDFSVHNGELGPT 650 HSP 2 Score: 369.392 bits (947), Expect = 5.352e-110 Identity = 223/628 (35.51%), Postives = 332/628 (52.87%), Query Frame = 0
Query: 648 DGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKV----QPILENIKHLL--KVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNT-----TGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGE-----------------------------------------------NIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
DG V++R K P+++P F + + + G A+A K + W ++QYY R AA+A IKLGL+ FH V I FN EW+I++LGAI AGG G+Y +NSA +Y++ N++ +L+VEN+ LQK+ Q +E +K ++ K+ V E + + W D +ELG + + QL Q + + ANQC IIYTSGT GNPKGVM+SHD +T+TA V + + K+ +VSYLPLSH+AA +D+++ + +YFA AL G+L+ TL EV+PT F GVPR++EK QE I+E +K +K + + + NT + + +++ K++++LGLDQCQ G APL P T++FFLSLDI I YG+SE G + + + YK +CG I + + GE I ILI +N PV +SN + +GD KF+S+ LT K D NG+P +LS ++C LG STV+ I+ Q P V AIQ+GID N+ +SNA R++KW ++ ++ S+ NGE+GPT K++R+
Sbjct: 34 DGEVLLRLSKHGPGHETPLTIPEFFRESVSRFGMYPALATKNSEHWEVLNFNQYYEACRKAARALIKLGLQRFHGVGILGFNSVEWLIASLGAILAGGLCVGIYATNSADACQYVITNAKVNVLLVENDLQLQKILSIPQSRMETLKAIIQYKLPVKESNNNLYS-----WNDFMELGNSIPDSQLDQIIESQRANQCAVIIYTSGTLGNPKGVMLSHDNITWTAGAVAKNCSLSNAAEKQEVVVSYLPLSHVAAQMMDVWIPMKIGAFIYFAQPDALTGTLINTLLEVKPTAFLGVPRIWEKMQEKIKESGAKCSSLRKKVFSWGRIIGLKVNTKRMFGVHDTTMSYRVAKALVFSKVRNALGLDQCQIPISGAAPLNPETSEFFLSLDIPIGEMYGMSESTGPHTTSSRNNYKIHSCGKIMSGCKNMLYQQSKDGTGEICIWGRHVFMGYLEMEDATMEAIDEEGWLHTGDLGRVDSHGFLYITGRIKEILITAGGENVAPVPIENMVKEKIPIISNAILVGDKAKFLSILLTLKCEVDRRNGEPLDKLSLEAIQFCRKLGSHVSTVSEILELQDPLVYKAIQQGIDAVNQEAISNAQRIQKWVILEKDFSVHNGELGPTTKIRRH 656 HSP 3 Score: 117.472 bits (293), Expect = 2.546e-25 Identity = 56/133 (42.11%), Postives = 88/133 (66.17%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG EN+AP+ IE+ +K +P++SN + +GD KF+S+ LT K D+ NG+P +LS +C L STV+ I+ + V AIQ+GID N+ A+SNA +++KW ++ ++ S+ NGE+GPT
Sbjct: 518 KEILITAGGENVAPVPIENMVKEKIPIISNAILVGDKAKFLSILLTLKCEVDRRNGEPLDKLSLEAIQFCRKLGSHVSTVSEILELQDPLVYKAIQQGIDAVNQEAISNAQRIQKWVILEKDFSVHNGELGPT 650
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG2 "Long-chain-fatty-acid--CoA ligase ACSBG2" species:9606 "Homo sapiens" [GO:0001676 "long-chain fatty acid metabolic process" evidence=IDA] [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IDA] [GO:0005524 "ATP binding" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0005739 "mitochondrion" evidence=IDA] [GO:0005829 "cytosol" evidence=IEA] [GO:0006631 "fatty acid metabolic process" evidence=IDA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0007283 "spermatogenesis" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0047617 "acyl-CoA hydrolase activity" evidence=IDA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 GO:GO:0007275 GO:GO:0005829 GO:GO:0005739 GO:GO:0005524 GO:GO:0030154 GO:GO:0016020 InterPro:IPR020845 GO:GO:0004467 eggNOG:COG1022 GO:GO:0007283 EMBL:CH471139 HOVERGEN:HBG054660 KO:K15013 OrthoDB:EOG7B5WVB CTD:81616 EMBL:AY009107 EMBL:AJ577571 EMBL:AL136854 EMBL:AY358766 EMBL:AK057412 EMBL:AK093433 EMBL:BC022027 EMBL:BC090046 RefSeq:NP_112186.3 RefSeq:XP_005259709.1 UniGene:Hs.465720 ProteinModelPortal:Q5FVE4 SMR:Q5FVE4 BioGrid:123551 STRING:9606.ENSP00000252669 PhosphoSite:Q5FVE4 DMDM:296434386 PaxDb:Q5FVE4 PRIDE:Q5FVE4 DNASU:81616 Ensembl:ENST00000252669 Ensembl:ENST00000586696 Ensembl:ENST00000588304 Ensembl:ENST00000588485 Ensembl:ENST00000591403 GeneID:81616 KEGG:hsa:81616 UCSC:uc002mee.1 GeneCards:GC19P006135 H-InvDB:HIX0014690 H-InvDB:HIX0014691 HGNC:HGNC:24174 HPA:HPA043421 HPA:HPA051013 MIM:614363 neXtProt:NX_Q5FVE4 PharmGKB:PA142672649 InParanoid:Q5FVE4 OMA:QRANQCA PhylomeDB:Q5FVE4 BioCyc:MetaCyc:HS05377-MONOMER ChiTaRS:ACSBG2 GeneWiki:ACSBG2 GenomeRNAi:81616 NextBio:71958 PRO:PR:Q5FVE4 ArrayExpress:Q5FVE4 Bgee:Q5FVE4 CleanEx:HS_ACSBG2 Genevestigator:Q5FVE4 GO:GO:0047617 Uniprot:Q5FVE4) HSP 1 Score: 444.121 bits (1141), Expect = 4.793e-137 Identity = 245/594 (41.25%), Postives = 352/594 (59.26%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKN----QKKSQMKVAQM-FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIG 616
++P + G+ ALA W N++QYY + AK+ IKLGL+ F+ V I FN EWFI+ +GAI AGGL G+Y TNS+ +Y++ +++ IL+VEN Q L+ I I ++ K ++ + NL W D +ELGR +L + NQC LIYTSGTTG PKGVMLSHD IT++ A+ K T VVSYLPLSHIAA +DI+V + + YFA ALKG+LV TLKEV+PT+F GVP++WEK E +K+ S +KK+ + + FK S + V +++ L+F KVK +LGLD C FISG APL++ T +FFLSLDIP+ E YGLSE G +++++ +CG I+ + + + D I GEIC+ GR + MGYL +E T+EA D EG+ H TG +K++L+T+GGENV P P+E +K +P++SNA++VGD KF+S+ LT+K + N+ GEP +L + LG +TV EI+ + P V KAIQ+GI+ N+ A++NA +++KW IL ++ S+ GE+G
Sbjct: 51 MTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELG 643 HSP 2 Score: 360.147 bits (923), Expect = 1.003e-106 Identity = 232/636 (36.48%), Postives = 338/636 (53.14%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKI-SIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKL----KKSQKFITNI-----LKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIF----------------------------------PSTQGRKKELLITAG--GE-----------NIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFKVN-SDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
K E TT DG V++R K PM++P F + + + G+ A+A K W ++QYY R AAK+ IKLGL+ FH V I FN EW I+ +GAI AGG G+Y +NSA + +Y++ +++ IL+VEN+ LQK+ I ++ LK ++ + W D +ELG++ + QL+Q + + ANQC +IYTSGTTG PKGVM+SHD +T+ A V + T K ++VSYLPLSHIAA +DI+V I YFA ALKG+LV TLKEV+PTVF GVP+++EK E +++ +K KK+ + NI K YNT + + K L ++ K+K SLGLD C F G APL T +FFLSLDI I YGLSE G + + Y+ +CG I +T+ E + +G G+ +I ILI +N P+ +SN + +GD KF+S+ LT K + +G+P +L+ +C LG +ASTVT I++ Q P V AIQ+GI+ N+ ++NA R++KW ++ ++ S+ GE+GP +KLKR+
Sbjct: 20 KTEVTPRLWTTCRDGEVLLRLSKHGPGHETPMTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTL----VFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNMLFQQNKDGIGEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRH 651 HSP 3 Score: 104.375 bits (259), Expect = 2.614e-21 Identity = 50/134 (37.31%), Postives = 89/134 (66.42%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+ PI +E +K +P++SN + +GD KF+S+ LT K ++ +G+P +L+ ++C L +ASTVT I++ + V AIQ+GI+ N+ A++NA +++KW ++ ++ S+ GE+GP +
Sbjct: 513 KEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMM 646
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:ACSBG1 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0000038 "very long-chain fatty acid metabolic process" evidence=IEA] [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IEA] [GO:0031957 "very long-chain fatty acid-CoA ligase activity" evidence=IEA] [GO:0051384 "response to glucocorticoid" evidence=IEA] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 InterPro:IPR020845 GO:GO:0004467 GO:GO:0051384 CTD:23205 GeneTree:ENSGT00690000101725 KO:K15013 OrthoDB:EOG7B5WVB TreeFam:TF354286 GO:GO:0031957 GO:GO:0000038 EMBL:AADN03006101 RefSeq:XP_413747.3 UniGene:Gga.12240 Ensembl:ENSGALT00000005199 GeneID:415361 KEGG:gga:415361 Uniprot:F1NLD6) HSP 1 Score: 433.721 bits (1114), Expect = 4.583e-133 Identity = 248/628 (39.49%), Postives = 356/628 (56.69%), Query Frame = 0
Query: 18 QALASSYTTTKPDKGVHIRTESS-EFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGD-----QKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
Q S T+ D V +R ++S +V M K GS ALA +NG W K + +YY + AK+F+KLGL+ F++V I FN PEWFIS +GA+FAGG+ G+Y TNS Y+ +S+ I+VVEN + L I I + LK +V+ ++ NL ++ L+LG L+D ++ NQCC LIYTSGTTG PKG MLSHD IT+ + + D A+ +VSYLPLSHIAA D++ + +VYFA+ ALKGSL++TLKEV+PT GVPRVWEK E +K+ S + K+ S+ N ++ ++ D L+ K++ ALG C + G APL+ T FFL L+I + E YG+SE G C +++ +CG P K+V + D + GEIC GR V MGYL E T EAFD++G+ H TGR+KDL++T+GGENV P PIE+A+K LP+VSNA+V+GD +KF+S+ LT+K V + +PT LT+ + + +G TTV EI+ + + +AIQ+GI++ N A + ++KW +LPR+ S+ GE+G T+
Sbjct: 41 QEQTESLWTSFADGRVRLRIDNSCPQPPITVHQMFKESLEKYGSLNALASRKNGKWEKITFSEYYCLSRKAAKSFLKLGLERFHSVAILGFNSPEWFISAVGAVFAGGIVTGIYTTNSPEACHYIAYDSKTNIMVVENRKQLDKIMQIWNRLPHLKAVVLYKDSILERHPNLYTMEEFLKLGDDISDVTLDDIINSQKPNQCCVLIYTSGTTGKPKGAMLSHDNITWTSAHCSRAGDMQPAEVQQESIVSYLPLSHIAAQIYDLWTGIKWGEQVYFAEPDALKGSLINTLKEVQPTSHMGVPRVWEKIMEKLKDASAQSGFVKKKMLSWAMSVSLERNLNCSSSSDLKQFWTRLADYLVLAKIRSALGFSSCQKHFCGAAPLNTETLYFFLGLNITLYEAYGMSETTGPHCLSGPYNYRQHSCGKPAPGCRVKLV-NEDTEGNGEICFWGRTVFMGYLNMEDKTKEAFDEDGWLHSGDLGKLDKDGFLYVTGRIKDLIITAGGENVPPIPIEDAVKKELPIVSNAMVIGDKKKFLSMLLTLKSVLDPDTSDPTDILTEQARDFCQKIGSKATTVSEIVATRDQAIYQAIQEGINKVNMNATNRVHCIQKWIVLPRDFSISGGELGPTM 667 HSP 2 Score: 286.96 bits (733), Expect = 6.239e-81 Identity = 168/431 (38.98%), Postives = 248/431 (57.54%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDLQP-MSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKL----KKSQKFITNI-LKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFP 1052
E E + T+ DG V +R QP ++V K + K GS A+A ++NG W + T+S+YY R AAK+F+KLGL+ FH+V+I FN PEW IS +GA+FAGG TG+Y +NS Y+ +S+ I+VVEN L K+ I + H LK VV+ +D + ++ + ++LG + L +++ NQCC +IYTSGTTG PKG M+SHD +T+T+ + ++ SIVSYLPLSHIAA D++ I ++YFA+ ALKGSL+ TLKEV+PT GVPRV+EK E +++ ++ KK + ++ L+ C +++ K+F L + ++ KI+ +LG CQ+ CG APL T FFL L+I + AYG+SE G P Y++ +CG P
Sbjct: 39 EDQEQTESLWTSFADGRVRLRIDNSCPQPPITVHQMFKESLEKYGSLNALASRKNGKWEKITFSEYYCLSRKAAKSFLKLGLERFHSVAILGFNSPEWFISAVGAVFAGGIVTGIYTTNSPEACHYIAYDSKTNIMVVENRKQLDKIMQIWNRLPH-LKAVVLYKDSILERHPNLYTMEEFLKLGDDISDVTLDDIINSQKPNQCCVLIYTSGTTGKPKGAMLSHDNITWTSAHCSRAGDMQPAEVQQESIVSYLPLSHIAAQIYDLWTGIKWGEQVYFAEPDALKGSLINTLKEVQPTSHMGVPRVWEKIMEKLKDASAQSGFVKKKMLSWAMSVSLERNLNCSSSSDLKQFWTRLADYLVLAKIRSALGFSSCQKHFCGAAPLNTETLYFFLGLNITLYEAYGMSETTGPHCLSGPYNYRQHSCGKPAP 468 HSP 3 Score: 125.176 bits (313), Expect = 8.723e-28 Identity = 62/147 (42.18%), Postives = 97/147 (65.99%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
F GR K+L+ITAGGEN+ PI IED +K LP++SN + IGD KKF+S+ LT K ++ DT+ P+ L+ +++C +G +A+TV+ I+ + + AIQ+GI+ N + ++KW ++PR+ S+ GE+GPT+KLKR
Sbjct: 526 FLYVTGRIKDLIITAGGENVPPIPIEDAVKKELPIVSNAMVIGDKKKFLSMLLTLKSVLDPDTS-DPTDILTEQARDFCQKIGSKATTVSEIVATRDQAIYQAIQEGINKVNMNATNRVHCIQKWIVLPRDFSISGGELGPTMKLKR 671 HSP 4 Score: 112.464 bits (280), Expect = 9.591e-24 Identity = 55/134 (41.04%), Postives = 85/134 (63.43%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K L ITAG EN+ PI IED +K LP++SN + IGD KKF+S+ LT K D + P+ L+ +D+C + +A+TV+ I+ + + AIQ+GI+K N A + ++KW ++PR+ S+ GE+GPT+
Sbjct: 534 KDLIITAGGENVPPIPIEDAVKKELPIVSNAMVIGDKKKFLSMLLTLKSVLDPDTSDPTDILTEQARDFCQKIGSKATTVSEIVATRDQAIYQAIQEGINKVNMNATNRVHCIQKWIVLPRDFSISGGELGPTM 667
BLAST of EMLSAG00000003336 vs. GO
Match: - (symbol:Acsbg2 "acyl-CoA synthetase bubblegum family member 2" species:10116 "Rattus norvegicus" [GO:0001676 "long-chain fatty acid metabolic process" evidence=ISO] [GO:0003674 "molecular_function" evidence=ND] [GO:0003824 "catalytic activity" evidence=IEA] [GO:0004467 "long-chain fatty acid-CoA ligase activity" evidence=IEA;ISO] [GO:0005524 "ATP binding" evidence=IEA] [GO:0005575 "cellular_component" evidence=ND] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005739 "mitochondrion" evidence=IEA;ISO] [GO:0005829 "cytosol" evidence=IEA;ISO] [GO:0006631 "fatty acid metabolic process" evidence=ISO] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0007283 "spermatogenesis" evidence=IEA] [GO:0008150 "biological_process" evidence=ND] [GO:0016020 "membrane" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0047617 "acyl-CoA hydrolase activity" evidence=IEA;ISO] InterPro:IPR000873 Pfam:PF00501 Prosite:PS00455 RGD:1588580 GO:GO:0007275 GO:GO:0005829 GO:GO:0005739 GO:GO:0005524 GO:GO:0030154 GO:GO:0016020 InterPro:IPR020845 GO:GO:0004467 GO:GO:0007283 GeneTree:ENSGT00690000101725 HOVERGEN:HBG054660 KO:K15013 OrthoDB:EOG7B5WVB CTD:81616 OMA:QRANQCA GO:GO:0047617 EMBL:BC129110 RefSeq:NP_001073565.1 UniGene:Rn.109833 ProteinModelPortal:A1L1K7 PRIDE:A1L1K7 Ensembl:ENSRNOT00000074555 GeneID:301120 KEGG:rno:301120 NextBio:648177 PRO:PR:A1L1K7 Genevestigator:A1L1K7 Uniprot:A1L1K7) HSP 1 Score: 419.083 bits (1076), Expect = 5.960e-128 Identity = 234/600 (39.00%), Postives = 351/600 (58.50%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKN-VTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDW---TTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKE---QSKNQKKSQMKVAQM--FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
++P + + G+ ALA W + QYY + A++ IKLGL F+ V I FN EW ++ LGAI AGGL G+Y TNS+ +Y++ + +L+VEN Q L+ I I + + T+K IV NL W+D +ELG +L+ + NQC +IYTSGTTG+PKGVMLSHD IT+ A+ ++ + + + +VSYLPLSHIAA +DI++ + + +FA AL+G+LV TL+EV+PT F GVPRVWEK Q+ IKE +S N +K A+M K + D + +++ L+F KV+ +LGLD C F SG +PLS+ +FFLSLDIP+ E YG++EC G KS ++ +CG ++ + K + D + GE+C+ GR V MGYL E AT E D++G+ H TGR+K++L+T+GGEN+ P PIE +K +P++S+A++VGD KF+S+ LT+K ++++ GEP +L + LG TV +IL + P V AIQ GID N++A+S++ +++KW IL ++ S++ GE+G T
Sbjct: 47 MTIPELFQESVERFGAYPALASKNGKKWDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALGAILAGGLCVGIYATNSAEACQYVIKQANVNVLIVENDQQLQKILSIPPDKMETVKAIVQYRLPLMENSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTGSPKGVMLSHDNITWTAGAMAREIELIHVSGKQDTIVSYLPLSHIAAQLMDIWIPIKVGVLTFFAQPDALRGTLVYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAFAWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNCHTFFSGASPLSQDVSEFFLSLDIPIGEIYGMTECSGPHTVSCKSIYRVLSCGKVLNGCKNMLYKQNKDGV-GEVCMWGRHVFMGYLGKEDATLEVLDEDGWLHSGDIGRLDSHDFLYITGRIKEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPT 645 HSP 2 Score: 375.555 bits (963), Expect = 3.440e-112 Identity = 229/636 (36.01%), Postives = 342/636 (53.77%), Query Frame = 0
Query: 639 AKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEK----HGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVI-----------------KVAYGLSECG---------------------------IFSDFAPSE------------YKKGTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
A +T DG V++R K PM++P + + + G+ A+A K W T+SQYY R AA++ IKLGL+ FH V I FN EW+++ LGAI AGG G+Y +NSA +Y++ + +L+VEN+ LQK+ I + +K +V + + ++ W+D +ELG QL + + + ANQC IIYTSGTTG+PKGVM+SHD +T+TA + + H K+ +IVSYLPLSHIAA +DI++ I +FA AL+G+LV TL+EV+PT F GVPRV+EK Q+ I+E ++K +K K NT G + N + +++ K++ SLGLD C F G +PL ++FFLSLDI I K Y + CG +F + E G G + F GR KE+LITAGGENI PI IE +K +P++S+ + +GD KF+S+ LT K +D +G+P +L+ +C LG +A TV++I++ + P V +AIQ GID+ N+ VS++ R++KW ++ ++ S+ GE+GPT KLKR+ ++
Sbjct: 20 APRLWSTHCDGEVLLRLSKHGPGHETPMTIPELFQESVERFGAYPALASKNGKKWDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALGAILAGGLCVGIYATNSAEACQYVIKQANVNVLIVENDQQLQKILSIPPDKMETVKAIVQYRLPLMENSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTGSPKGVMLSHDNITWTAGAMAREIELIHVSGKQDTIVSYLPLSHIAAQLMDIWIPIKVGVLTFFAQPDALRGTLVYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAFAWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNCHTFFSGASPLSQDVSEFFLSLDIPIGEIYGMTECSGPHTVSCKSIYRVLSCGKVLNGCKNMLYKQNKDGVGEVCMWGRHVFMGYLGKEDATLEVLDEDGWLHSGDIGRLDSHDFLYITGRIKEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPTSKLKRDLITQ 655 HSP 3 Score: 104.76 bits (260), Expect = 2.005e-21 Identity = 52/133 (39.10%), Postives = 89/133 (66.92%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG ENI PI IE +K +P++S+ + +GD KF+S+ LT K +D+ +G+P +L+ +C L +A TV++I++ + V +AIQ GID N+ AVS++ +++KW ++ ++ S+ GE+GPT
Sbjct: 513 KEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPT 645
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592768284|gb|GAXK01186284.1| (TSA: Calanus finmarchicus comp44223_c0_seq1 transcribed RNA sequence) HSP 1 Score: 480.715 bits (1236), Expect = 1.068e-148 Identity = 259/657 (39.42%), Postives = 391/657 (59.51%), Query Frame = 0
Query: 1 MEAEHSNSMTDKMF------PNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAI--MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIP-STHAKVVKSSDDDI--GGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFIS 627
+ +H N++ D+ + P++ ++ T P V +R E S S SVP+ +K AL ++ G W++ YY D++ KAFI LGL F +VCI FN PEW IS LGAIFAGGL+AG+YPTN + +Y++++S+C ILVVE+ + L I I + LKKIV + G +I WKDL+ G+ G D L RL I+VNQCC+L+YTSGTTGNPK VM+SHD +T+ TK H + F ++SYLPLSHIA + +DI+ +Y+ G YFADK +K +L+D +PT+F GVPRVWEK QE + E++K+ K + +++ K + Y++ GD++ + K+ K+ + KVK LG D+C F +G AP+ +T ++F+SLD+ + E YG+SE + Q +D + I P +K+V SSD ++ E+ + GR +MMGYL E AT + ++G+ T GR KDL++T+GGEN+AP I + +K LP++S +++GD +KF+S LT+ V+ N D EPT++L+ + + +++G N T+ +IL VMK +Q GID AN++A+SNA K++KW ILPR+ SL GE+G T+ G ++
Sbjct: 485 VRVKHHNNIMDEDYSEFVNGPDQVIPCNNRITWVPAGTVKLRIEGSGAASDAPISVPSRMKQTATENPELPALKTRDVKTGQEKVWSWSAYYQDVRTVGKAFIDLGLKRFDSVCILGFNTPEWNISYLGAIFAGGLAAGIYPTNGTDACKYILDHSQCSILVVEDQKQLDKIWSIRNELPHLKKIV--QYSGIPNNAGVISWKDLMIRGKTLGDDELEGRLRRIAVNQCCTLVYTSGTTGNPKAVMMSHDNLTWNTKTACTIHKFEKRNFRILSYLPLSHIAGNMVDIHAPMYMAGTTYFADKNVMKSTLLDNTNWCKPTVFLGVPRVWEKIQEKMLEKAKDVKGLKKTISKQAKLTGLKYHQEGDKEGLFKVFQKIYYSKVKALLGFDQCKWFFTGAAPIEAKTMNYFMSLDMRILELYGMSESTGMHTSQLTDMQKPKSVGITPEGVTSKLVPSSDAEVTDSEELWMSGRHIMMGYLNREDATRKDMTEDGWLKTGDLVSIDQDGFHFIVGREKDLIITAGGENIAPQTIHDTVKDKLPIISQVLLLGDKQKFVSTFLTLSVEVNTDTMEPTNKLSSAARDWCRSVGSNANTIEDILRGPDHRVMKGLQAGIDAANRQAVSNAQKIQKWMILPRDFSLPGGEMGPTMKVKRGHVT 2449 HSP 2 Score: 436.032 bits (1120), Expect = 2.678e-132 Identity = 246/628 (39.17%), Postives = 355/628 (56.53%), Query Frame = 0
Query: 644 TTLPDGSVIIRRKKDDLQ---PMSVPSFVKHNCLKGGSQIAMAIK--RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKK-ISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAY----------------------GLSECGIFSDFAPSE-------------------------------------YKKGTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTF--KVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
T +P G+V +R + P+SVPS +K + A+ + + G W++S YY+++R KAFI LGLK F +V I FN PEW IS LGAIFAGG A G+YP+N +Y++++S+C ILVVE+ L K+ I + HL K+V + G + I W+DL+ GKT +D+L+ RL IA NQCC+++YTSGTTGNPK VM+SHD LT+ KT H + K+ I+SYLPLSHIA N +DI+ + G YFADK+ +K +L+ +PTVF GVPRV+EK QE + EK +K +K I+ K Y+ G+K+ + + ++ Y K+K LG DQC+ F G AP+ T ++F+SLD+ I Y G++ G+ S PS K G SI F GR+K+L+ITAGGENIAP I D +K+ LP++S V+ +GD +KF+S FLT +VN+DT +P+ +LS++ ++WC S+G A+T+ +I+R VM +Q GID AN VSNA +++KW ++PR+ S+ GEMGPT+K+KR +K
Sbjct: 482 TWVPAGTVKLRIEGSGAASDAPISVPSRMKQTATENPELPALKTRDVKTGQEKVWSWSAYYQDVRTVGKAFIDLGLKRFDSVCILGFNTPEWNISYLGAIFAGGLAAGIYPTNGTDACKYILDHSQCSILVVEDQKQLDKIWSIRNELPHLKKIV--QYSGIPNNAGVISWKDLMIRGKTLGDDELEGRLRRIAVNQCCTLVYTSGTTGNPKAVMMSHDNLTWNTKTACTIHKFEKRNFRILSYLPLSHIAGNMVDIHAPMYMAGTTYFADKNVMKSTLLDNTNWCKPTVFLGVPRVWEKIQEKMLEKAKDVKGLKKTISKQAKLTGLKYHQEGDKEGLFKVFQKIYYSKVKALLGFDQCKWFFTGAAPIEAKTMNYFMSLDMRILELYGMSESTGMHTSQLTDMQKPKSVGITPEGVTSKLVPSSDAEVTDSEELWMSGRHIMMGYLNREDATRKDMTEDGWLKTGDLVSIDQDGFHFIVGREKDLIITAGGENIAPQTIHDTVKDKLPIISQVLLLGDKQKFVSTFLTLSVEVNTDTM-EPTNKLSSAARDWCRSVGSNANTIEDILRGPDHRVMKGLQAGIDAANRQAVSNAQKIQKWMILPRDFSLPGGEMGPTMKVKRGHVTK 2356 HSP 3 Score: 137.117 bits (344), Expect = 8.659e-32 Identity = 69/148 (46.62%), Postives = 101/148 (68.24%), Query Frame = 0
Query: 1301 FRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTF--KVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
F ++V GR EK L ITAG ENIAP I D +K+ LP++S V+ +GD +KF+S FLT +VN+D +P+ +LS+ +DWC S+ A+T+ +I+R V+ +Q GID AN+ AVSNA K++KW ++PR+ S+ GEMGPT+
Sbjct: 509 FHFIV----GR-EKDLIITAGGENIAPQTIHDTVKDKLPIISQVLLLGDKQKFVSTFLTLSVEVNTD-TMEPTNKLSSAARDWCRSVGSNANTIEDILRGPDHRVMKGLQAGIDAANRQAVSNAQKIQKWMILPRDFSLPGGEMGPTM 934 HSP 4 Score: 46.2098 bits (108), Expect = 9.298e-4 Identity = 21/39 (53.85%), Postives = 25/39 (64.10%), Query Frame = 0
Query: 1205 IYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIY 1243
I FN PEW IS LG +FAGG A G+Y +N T +C Y
Sbjct: 1997 ILGFNTPEWNISYLGAIFAGGLAAGIYPTNGTD--ACKY 2107
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592950611|gb|GAXK01007942.1| (TSA: Calanus finmarchicus comp136906_c1_seq1 transcribed RNA sequence) HSP 1 Score: 458.759 bits (1179), Expect = 7.729e-141 Identity = 257/659 (39.00%), Postives = 376/659 (57.06%), Query Frame = 0
Query: 4 EHSNSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFL--------SFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE------NGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDI-----GGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVK-SNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFI 626
+ + S T + P++ SYT++ P V + E SVP ++ ALA+ R+G WVKW Y++Y D+++ KAFIKLGL + V I FN PEWF S+LG +FAGGL+ G+YPTNS +Y++ N + I+VVE+ + L I + ++ LK I I+ G E +I W++++++G E D L R+S+++ N CC L+YTSGTTG PKGVMLSHD +TF + +V+ + + VSYLPLSH+AA+ +D+++ G +YFA++ ALKG+LV TLKE PT+FFGVPRVWEK +E + + K + V K + Y + G + KI DK++F KVK+ LG + F S AP+S ++F+SLDI + E YG+SE G F+ GT G +P H ++ K + GE+C++GR V MGYL E+ T EAFD +G+ H TGR+K++L+T+GGENV P IE+ +K LP +S+ V++GD KF+S +T++V+ NE EP S+L + K+ G T+ ++L VM+AIQ GID NK+A SNA K++KW ILP + S+ GEIG T+ FI
Sbjct: 412 DDTRSTTSETGPDRLLPGDSYTSSDPGVPVKLWMPEDEEDCDEEDLRTPLSVPTLLIRAAEDAPDHIALAVKRDGAWVKWTYERYLEDVRVVGKAFIKLGLKVGHGVGIIGFNSPEWFFSDLGCVFAGGLATGIYPTNSPEACKYVLANCKANIVVVEDDKQLAKILDVKDSLPELKHI--IQYTGNPSVEGVISWREVMDMGAAEDDDELESRISNLAPNMCCHLVYTSGTTGPPKGVMLSHDNLTFTARTLVEHYGLQDMEERFVSYLPLSHVAANIMDLFMVTQCLGTLYFANRDALKGTLVATLKEASPTVFFGVPRVWEKVREKMVQVGKANTGFKRAVGIWAKKTGLDYNKAKIGGRRGRGSLSFKIADKMVFTKVKEELGFGQTHSFFSAAAPISTEVLEYFMSLDIKILEIYGMSEISGPQTGNDYEYFRPGTIGKDLPGFHTRLDKEAPGVAEICPGAGELCMRGRNVFMGYLGAEEKTREAFDTDGWHHSGDVGTVDEGGFYTITGRIKEILITAGGENVPPFIIEDMVKKELPCLSHVVLIGDRRKFLSCLVTLRVEVDNETLEPKSKLERDTLEWVKDQGSKAETLDDVLRGPDTIVMEAIQAGIDRYNKKANSNAQKIQKWTILPTDFSIPGGEIGPTMKVKRHFI 2382 HSP 2 Score: 326.635 bits (836), Expect = 5.808e-94 Identity = 169/405 (41.73%), Postives = 246/405 (60.74%), Query Frame = 0
Query: 662 PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNI-LKDAAFCYNTT------GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEY-KKGTCGSIFPSTQGR 1057
P+SVP+ + IA+A+KR+G W++WTY +Y ++R+ KAFIKLGLK H V I FN PEW S+LG +FAGG ATG+YP+NS +Y++ N + I+VVE++ L K+ + +++ L +I+ G + + I WR+++++G E++D+L+ R+ N+A N CC ++YTSGTTG PKGVM+SHD LT+TA+T+VE +G + VSYLPLSH+AAN +D+++V C G LYFA++ ALKG+LV TLKE PTVFFGVPRV+EK +E + + + K K I K YN G + ++M++ K+K+ LG Q F AP+ ++F+SLDI I YG+SE EY + GT G P R
Sbjct: 556 PLSVPTLLIRAAEDAPDHIALAVKRDGAWVKWTYERYLEDVRVVGKAFIKLGLKVGHGVGIIGFNSPEWFFSDLGCVFAGGLATGIYPTNSPEACKYVLANCKANIVVVEDDKQLAKILDVKDSLPELKH--IIQYTGNPSVEGVISWREVMDMGAAEDDDELESRISNLAPNMCCHLVYTSGTTGPPKGVMLSHDNLTFTARTLVEHYGLQDMEERFVSYLPLSHVAANIMDLFMVTQCLGTLYFANRDALKGTLVATLKEASPTVFFGVPRVWEKVREKMVQ-VGKANTGFKRAVGIWAKKTGLDYNKAKIGGRRGRGSLSFKIADKMVFTKVKEELGFGQTHSFFSAAAPISTEVLEYFMSLDIKILEIYGMSEISGPQTGNDYEYFRPGTIGKDLPGFHTR 1761 HSP 3 Score: 133.65 bits (335), Expect = 1.192e-30 Identity = 67/160 (41.88%), Postives = 101/160 (63.12%), Query Frame = 0
Query: 1042 YKKGTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
+ G G++ F + GR KE+LITAGGEN+ P +IED +K LP LS+VV IGD +KF+S +T +V D +P ++L EW G +A T+ +++R VM AIQ GID N+ SNA +++KW+++P + S+ GE+GPT+K+KR+
Sbjct: 1897 HHSGDVGTVDEGGFYTITGRIKEILITAGGENVPPFIIEDMVKKELPCLSHVVLIGDRRKFLSCLVTLRVEVDNETLEPKSKLERDTLEWVKDQGSKAETLDDVLRGPDTIVMEAIQAGIDRYNKKANSNAQKIQKWTILPTDFSIPGGEIGPTMKVKRH 2376 HSP 4 Score: 115.546 bits (288), Expect = 4.028e-25 Identity = 56/134 (41.79%), Postives = 86/134 (64.18%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+ P +IED +K LP LS+VV IGD +KF+S +T +V D +P ++L +W +A T+ +++R V+ AIQ GID+ NK A SNA K++KW+++P + S+ GE+GPT+
Sbjct: 1960 KEILITAGGENVPPFIIEDMVKKELPCLSHVVLIGDRRKFLSCLVTLRVEVDNETLEPKSKLERDTLEWVKDQGSKAETLDDVLRGPDTIVMEAIQAGIDRYNKKANSNAQKIQKWTILPTDFSIPGGEIGPTM 2361 HSP 5 Score: 47.3654 bits (111), Expect = 3.883e-4 Identity = 22/40 (55.00%), Postives = 27/40 (67.50%), Query Frame = 0
Query: 1204 NIYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIY 1243
I FN PEW S+LG VFAGG ATG+Y +NS ++C Y
Sbjct: 733 GIIGFNSPEWFFSDLGCVFAGGLATGIYPTNSP--EACKY 846
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592837595|gb|GAXK01119949.1| (TSA: Calanus finmarchicus comp353788_c0_seq2 transcribed RNA sequence) HSP 1 Score: 435.647 bits (1119), Expect = 3.374e-133 Identity = 255/639 (39.91%), Postives = 370/639 (57.90%), Query Frame = 0
Query: 15 PNKQALASSYTTTKPDKGVHIRTE---SSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE---NG--DQKVIHKIGDKLIFQKVKQALGLDRCLE-----FISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKS-----DFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
P++ A ++TTT+PD V IR SS SV +M++ ++TALA+ R+G WVKW Y +Y +I+ AKAFIKLGL P Y+V I N PEWFISN+ AI AGGL+ G+Y TNS +Y+ +SR ++VVE+ L+ + + ++ L+ I+ + +PG ++ WK+LL++G L RL + +VNQ C ++YTSGTTGNPKGVMLS D IT++ + +W+ + VSYLPLSHIAA +DIY+ + V+FADK AL+G+L+ TL EVRPT F VPRV+EK QE + E KN K + K++ K S+ ++ NG +K+ KLI KV + LGLDR SG APLS +T +F SLD+P+ E G SE C Q + + G+ G P ++ S D + GEI KGR + +GYL+ ++ T E D +G+ H +GR+K++++T+GGEN+AP PIE IK + ++SNA+VVGD K ++ +T+K +E +PTS L ++VK K G T E++ V I +GID AN+ AIS A +V+K+ I P + SL++GE+ TL
Sbjct: 186 PDRILSADAFTTTQPDGAVKIRINGHPSSRNPPKSVWSMIQETVKRAPNQTALAVKRDGVWVKWTYAEYEKEIRTVAKAFIKLGLKPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNSPDAVKYVAKHSRANLMVVEDEVQLKKVQLVQDDLDNLEAIIQYTGEPSQPG--VVSWKELLKIGNTVSDSVLTMRLENQAVNQPCMVVYTSGTTGNPKGVMLSQDNITWIIQVSQDTFNWSFDQEHAVSYLPLSHIAAQVIDIYLTFFGGATVWFADKDALQGTLLKTLLEVRPTRFIAVPRVYEKMQEKMMEIGKNNKGFKKKLSDRAKSVSLDHHNEIMNGGAGNSFEYKLVQKLILSKVHKRLGLDRAAHPSRGGLYSGAAPLSIQTFQYFQSLDLPIMELLGSSET---CGPQTACLPGIGTRIGSVGRCFPQFETNILNSEQDGV-GEIITKGRQICLGYLWEKEKTDELIDSDGWVHSGDLGRVDGDGFFYVSGRMKEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAEEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTL 2084 HSP 2 Score: 393.66 bits (1010), Expect = 6.268e-118 Identity = 236/643 (36.70%), Postives = 347/643 (53.97%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCY-----NTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGG-----APLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPS-EYKKGTCGSIFPS-----------------TQGRK------------------------------------------KELLITAGGENIAPILIEDNIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
++I A +TTT PDG+V IR P SV S ++ + +Q A+A+KR+G W++WTY++Y +EIR AKAFIKLGLKP ++V I N PEW ISN+ AI AGG ATG+Y +NS +Y+ +SR ++VVE+ L+KVQ + +++ +L +I+ G + + W++L+++G T + L RL N A NQ C ++YTSGTTGNPKGVM+S D +T+ + + W+ + VSYLPLSHIAA +DIY+ ++FADK AL+G+L++TL EVRPT F VPRVYEK QE + E K +K +++ K + + N F L +++ K+ LGLD+ S GG APL T +F SLD+ I G SE CG + P + G+ G FP T+GR+ KE++ITAGGENIAP+ IE+ IK + ++SN + +GD +K ++ +T K D QP++ L +VK W G +A T +I + V + I +GID AN+ +S A RV+K+ + P + S+D+GE+ PTLK+KR+ K
Sbjct: 159 DRILSADAFTTTQPDGAVKIRINGHPSSRNPPKSVWSMIQETVKRAPNQTALAVKRDGVWVKWTYAEYEKEIRTVAKAFIKLGLKPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNSPDAVKYVAKHSRANLMVVEDEVQLKKVQLVQDDLDNL--EAIIQYTGEPSQPGVVSWKELLKIGNTVSDSVLTMRLENQAVNQPCMVVYTSGTTGNPKGVMLSQDNITWIIQVSQDTFNWSFDQEHAVSYLPLSHIAAQVIDIYLTFFGGATVWFADKDALQGTLLKTLLEVRPTRFIAVPRVYEKMQEKMMEIGKNNKGFKKKLSDRAKSVSLDHHNEIMNGGAGNSFEYKLVQKLILSKVHKRLGLDRAAHPSRGGLYSGAAPLSIQTFQYFQSLDLPIMELLGSSETCGPQTACLPGIGTRIGSVGRCFPQFETNILNSEQDGVGEIITKGRQICLGYLWEKEKTDELIDSDGWVHSGDLGRVDGDGFFYVSGRMKEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAEEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTLKIKRHSIVK 2081 HSP 3 Score: 100.908 bits (250), Expect = 1.498e-20 Identity = 51/134 (38.06%), Postives = 83/134 (61.94%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG ENIAP+ IE+ IK + ++SN + +GD +K ++ +T K D+ QP++ L VK W +A T +I + V + I +GID+AN+ A+S A +V+K+ + P + S+D+GE+ PTL
Sbjct: 186 KEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAEEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTL 587 HSP 4 Score: 43.1282 bits (100), Expect = 7.035e-3 Identity = 19/38 (50.00%), Postives = 25/38 (65.79%), Query Frame = 0
Query: 1200 KIHFN--IYSFNFPEWMISNLGPVFAGGFATGLYLSNS 1235
K H++ I N PEW ISN+ + AGG ATG+Y +NS
Sbjct: 1716 KPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNS 1829
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592837596|gb|GAXK01119948.1| (TSA: Calanus finmarchicus comp353788_c0_seq1 transcribed RNA sequence) HSP 1 Score: 436.802 bits (1122), Expect = 1.512e-132 Identity = 255/639 (39.91%), Postives = 370/639 (57.90%), Query Frame = 0
Query: 15 PNKQALASSYTTTKPDKGVHIRTE---SSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE---NG--DQKVIHKIGDKLIFQKVKQALGLDRCLE-----FISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKS-----DFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
P++ A ++TTT+PD V IR SS SV +M++ ++TALA+ R+G WVKW Y +Y +I+ AKAFIKLGL P Y+V I N PEWFISN+ AI AGGL+ G+Y TNS +Y+ +SR ++VVE+ L+ + + ++ L+ I+ + +PG ++ WK+LL++G L RL + +VNQ C ++YTSGTTGNPKGVMLS D IT++ + +W+ + VSYLPLSHIAA +DIY+ + V+FADK AL+G+L+ TL EVRPT F VPRV+EK QE + E KN K + K++ K S+ ++ NG +K+ KLI KV + LGLDR SG APLS +T +F SLD+P+ E G SE C Q + + G+ G P ++ S D + GEI KGR + +GYL+ ++ T E D +G+ H +GR+K++++T+GGEN+AP PIE IK + ++SNA+VVGD K ++ +T+K +E +PTS L ++VK K G T E++ V I +GID AN+ AIS A +V+K+ I P + SL++GE+ TL
Sbjct: 459 PDRILSADAFTTTQPDGAVKIRINGHPSSRNPPKSVWSMIQETVKRAPNQTALAVKRDGEWVKWTYAEYEKEIRTVAKAFIKLGLKPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNSPDAVKYVAKHSRANLMVVEDEVQLKKVQLVQDDLDNLEAIIQYTGEPSQPG--VVSWKELLKIGNTVSDSVLTMRLENQAVNQPCMVVYTSGTTGNPKGVMLSQDNITWIIQVSQDTFNWSFDQEHAVSYLPLSHIAAQVIDIYLTFFGGATVWFADKDALQGTLLKTLLEVRPTRFIAVPRVYEKMQEKMMEIGKNNKGFKKKLSDRAKSVSLDHHNEIMNGGAGNSFEYKLVQKLILSKVHKRLGLDRAAHPSRGGLYSGAAPLSIQTFQYFQSLDLPIMELLGSSET---CGPQTACLPGIGTRIGSVGRCFPQFETNILNSEQDGV-GEIITKGRQICLGYLWEKEKTDELIDSDGWVHSGDLGRVDGDGFFYVSGRMKEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAKEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTL 2357 HSP 2 Score: 395.586 bits (1015), Expect = 1.358e-117 Identity = 236/643 (36.70%), Postives = 348/643 (54.12%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCY-----NTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGG-----APLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPS-EYKKGTCGSIFPS-----------------TQGRK------------------------------------------KELLITAGGENIAPILIEDNIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
++I A +TTT PDG+V IR P SV S ++ + +Q A+A+KR+G+W++WTY++Y +EIR AKAFIKLGLKP ++V I N PEW ISN+ AI AGG ATG+Y +NS +Y+ +SR ++VVE+ L+KVQ + +++ +L +I+ G + + W++L+++G T + L RL N A NQ C ++YTSGTTGNPKGVM+S D +T+ + + W+ + VSYLPLSHIAA +DIY+ ++FADK AL+G+L++TL EVRPT F VPRVYEK QE + E K +K +++ K + + N F L +++ K+ LGLD+ S GG APL T +F SLD+ I G SE CG + P + G+ G FP T+GR+ KE++ITAGGENIAP+ IE+ IK + ++SN + +GD +K ++ +T K D QP++ L +VK W G +A T +I + V + I +GID AN+ +S A RV+K+ + P + S+D+GE+ PTLK+KR+ K
Sbjct: 432 DRILSADAFTTTQPDGAVKIRINGHPSSRNPPKSVWSMIQETVKRAPNQTALAVKRDGEWVKWTYAEYEKEIRTVAKAFIKLGLKPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNSPDAVKYVAKHSRANLMVVEDEVQLKKVQLVQDDLDNL--EAIIQYTGEPSQPGVVSWKELLKIGNTVSDSVLTMRLENQAVNQPCMVVYTSGTTGNPKGVMLSQDNITWIIQVSQDTFNWSFDQEHAVSYLPLSHIAAQVIDIYLTFFGGATVWFADKDALQGTLLKTLLEVRPTRFIAVPRVYEKMQEKMMEIGKNNKGFKKKLSDRAKSVSLDHHNEIMNGGAGNSFEYKLVQKLILSKVHKRLGLDRAAHPSRGGLYSGAAPLSIQTFQYFQSLDLPIMELLGSSETCGPQTACLPGIGTRIGSVGRCFPQFETNILNSEQDGVGEIITKGRQICLGYLWEKEKTDELIDSDGWVHSGDLGRVDGDGFFYVSGRMKEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAKEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTLKIKRHSIVK 2354 HSP 3 Score: 100.523 bits (249), Expect = 1.839e-20 Identity = 51/134 (38.06%), Postives = 83/134 (61.94%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG ENIAP+ IE+ IK + ++SN + +GD +K ++ +T K D+ QP++ L VK W +A T +I + V + I +GID+AN+ A+S A +V+K+ + P + S+D+GE+ PTL
Sbjct: 459 KEIIITAGGENIAPVPIEECIKAEMEDIISNAMVVGDQRKHLAAIITLKTVVDEKNQPTSILHENVKTWLKRTGSKAQTAKEVIEENNERVFAEISEGIDRANQFAISKAHRVQKFFIAPSDFSLDSGELTPTL 860 HSP 4 Score: 43.8986 bits (102), Expect = 5.240e-3 Identity = 30/100 (30.00%), Postives = 44/100 (44.00%), Query Frame = 0
Query: 1142 RDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKI----HFN--IYSFNFPEWMISNLGPVFAGGFATGLYLSNS 1235
R+ P V S IQ+ + A R +W + E ++ F K+ H++ I N PEW ISN+ + AGG ATG+Y +NS
Sbjct: 1989 RNPPKSVWSMIQETVKRAPNQTALAVKRDGEW------VKWTYAEYEKEIRTVAKAFIKLGLKPHYSVGIIGHNAPEWFISNIAAIIAGGLATGIYATNS 2270
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592758401|gb|GAXK01196012.1| (TSA: Calanus finmarchicus comp532167_c0_seq1 transcribed RNA sequence) HSP 1 Score: 429.483 bits (1103), Expect = 1.188e-131 Identity = 251/637 (39.40%), Postives = 362/637 (56.83%), Query Frame = 0
Query: 21 ASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIM-RNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGD-----QKVIHKIGDKLIFQKVKQALGLDRCL--EFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKS-----DFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNL-GCNYTTVGEIL----YHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A + T PD V I+ + + S S+P M + S AL R+G +KW Y QY +++ AK F+ LGL F+TV I +N P+W I+N+ A+ AGG + G+Y TN+ + EY+ N+SR +LVVEN + L + I KN+ L+ IV E G E ++ W+ LL+LGR E L+ RL+ I+ NQC L+YTSGTTGNPKG MLSHD + + ++ W VVSYLPLSH+A S +DIY + FAD ALKG+LV+ +K +PT F GVPRVWEK +E IK K+ K + KVA K ++ ++ D + + +K+ K++ KV +A+G D+ + E +GGA S T FFLSLDI + + YG +E + C S + K GT G P + D+ GEI +GRC+ MGYLF+++ T E FD +G+ HT GR+K++++T+GGENVAP IE IK LP ++SN +VVG+ KF++ +T+KVK + E PT L D ++ K + G + TV ++L + K + AI +GI+ ANKRA+S AA V+KW I+PRE S+ GE+ +L
Sbjct: 107 AQDHRTWLPDGAVTIQMKDTGPQSKPAVSIPEMFHSTVEMFPSHLALHTRDRHGADLKWTYSQYMEEVRTAAKGFLALGLGQFHTVGILGYNSPQWAIANVAAVHAGGFATGIYQTNTPAACEYVANHSRANVLVVENKEQLDKVLSIRKNLPHLRAIVQYE--GIPEEEGVLSWQQLLQLGRLESDQELDGRLADIAANQCALLVYTSGTTGNPKGTMLSHDGVVMNSVEYTQQQGWMMGTERVVSYLPLSHVAGSMMDIYYPMSKGATTCFADSNALKGTLVENIKYYKPTRFLGVPRVWEKIEEKIKLAGKDTKGLKRKVADWAKKQALAHHLQKDAGIDRKSLGYKLAKKIVLSKVHEAIGFDQVVNQEMGTGGAVTSPETHTFFLSLDIRLLDVYGSTES-VGCPQTGSLPGPGNCKVGTVGRSTPEIMENKINKPDEKGEGEIFSRGRCIAMGYLFDKEKTLETFDDDGWLHTGDKGFIDADGFYTIVGRIKEIIITAGGENVAPVNIEGEIKKELPDIISNIMVVGEQRKFLTCLITLKVKPDPETLAPTDILEDDAREWVKEVSGEDVETVQQVLKLLEWDKWGKMAMAIDEGIERANKRAVSRAAMVQKWTIVPREFSVSGGELSPSL 2008 HSP 2 Score: 368.237 bits (944), Expect = 1.281e-109 Identity = 232/664 (34.94%), Postives = 348/664 (52.41%), Query Frame = 0
Query: 633 TEKIEDAKEYTTTLPDGSVIIRRKKDDLQ---PMSVPSFVKHNCLKGGSQIAMAIK-RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV----VIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKIS-IVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQC--QEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE---CGIFSDF-APSEYKKGTCGSIFPS------------------TQGR----------KKEL---------------LITAGG-----ENIAPILIEDNIKNFLPV-------------LSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSL-GYEASTVTNIIR----DQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFN 1204
+++I A+++ T LPDG+V I+ K Q +S+P S +A+ + R+G ++WTYSQY E+R AAK F+ LGL FHTV I +N P+W I+N+ A+ AGGFATG+Y +N+ + EY+ N+SR +LVVEN L KV I +N+ HL +V + EE+G ++ W+ L++LG+ E + +L RL +IAANQC ++YTSGTTGNPKG M+SHD + + ++ GW +VSYLPLSH+A + +DIY + FAD +ALKG+LV +K +PT F GVPRV+EK +E G++ K++ K Q ++ KDA + G K L +++ K+ +++G DQ QE GGA P T FFLSLDI + YG +E C P K GT G P ++GR +K L I A G I I+I +N PV +SN++ +G+ +KF++ +T KV D P+ L + +EW + G + TV +++ D+ ++ AI +GI+ AN+ VS AA V+KW+++PRE S+ GE+ P+LKLKR E +++ N
Sbjct: 89 SQRILPAQDHRTWLPDGAVTIQMKDTGPQSKPAVSIPEMFHSTVEMFPSHLALHTRDRHGADLKWTYSQYMEEVRTAAKGFLALGLGQFHTVGILGYNSPQWAIANVAAVHAGGFATGIYQTNTPAACEYVANHSRANVLVVENKEQLDKVLSIRKNLPHLRAIVQYEGIPEEEGVLS------WQQLLQLGRLESDQELDGRLADIAANQCALLVYTSGTTGNPKGTMLSHDGVVMNSVEYTQQQGWMMGTERVVSYLPLSHVAGSMMDIYYPMSKGATTCFADSNALKGTLVENIKYYKPTRFLGVPRVWEKIEEKIKLAGKDTKGLKRKVADWAKKQALAHHLQKDAGIDRKSLGYK-----LAKKIVLSKVHEAIGFDQVVNQEMGTGGAVTSPETHTFFLSLDIRLLDVYGSTESVGCPQTGSLPGPGNCKVGTVGRSTPEIMENKINKPDEKGEGEIFSRGRCIAMGYLFDKEKTLETFDDDGWLHTGDKGFIDADGFYTIVGRIKEIIITAGGENVAPVNIEGEIKKELPDIISNIMVVGEQRKFLTCLITLKVKPDPETLAPTDILEDDAREWVKEVSGEDVETVQQVLKLLEWDKWGKMAMAIDEGIERANKRAVSRAAMVQKWTIVPREFSVSGGELSPSLKLKRFEVMEMYKN 2047 HSP 3 Score: 104.76 bits (260), Expect = 6.909e-22 Identity = 56/140 (40.00%), Postives = 91/140 (65.00%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYE-ASTVTNIIR----DKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+AP+ IE +IK LP ++SN++ +G+ +KF++ +T KV D P+ L + ++W +S E TV +++ DK ++ AI +GI++ANK AVS AA V+KW+++PRE S+ GE+ P+L
Sbjct: 1589 KEIIITAGGENVAPVNIEGEIKKELPDIISNIMVVGEQRKFLTCLITLKVKPDPETLAPTDILEDDAREWVKEVSGEDVETVQQVLKLLEWDKWGKMAMAIDEGIERANKRAVSRAAMVQKWTIVPREFSVSGGELSPSL 2008 HSP 4 Score: 44.2838 bits (103), Expect = 3.250e-3 Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 0
Query: 1191 LKLKRNEFSKIHFNIYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIY 1243
L L +F + I +N P+W I+N+ V AGGFATG+Y +N+ + +C Y
Sbjct: 332 LALGLGQFHTV--GILGYNSPQWAIANVAAVHAGGFATGIYQTNTPA--ACEY 478
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592756207|gb|GAXK01198206.1| (TSA: Calanus finmarchicus comp338023_c0_seq1 transcribed RNA sequence) HSP 1 Score: 428.328 bits (1100), Expect = 5.395e-131 Identity = 253/666 (37.99%), Postives = 378/666 (56.76%), Query Frame = 0
Query: 12 KMFPNKQALASSYTTTKPDKGVHIRTE---SSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDR--LNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE-----NGDQKVIHKIGDKLIFQKVKQALGLDRCL-----EFISGGAPLSERTRDFFLSLDIPVQECYGLSECG--IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTT 644
K+ P++ A+S++TT D V IR+ SS++ + SV ++K + ALA+ R+G WVKW Y +Y+ D++ AKAFIK+GL + V I FN PEW IS+L ++ AGGLSAG+Y TNS+ Y++N+SR ILV+EN + ++ + +++ LK I+ PG ++ W +LLE+GR G+D L + L + NQ L+YTSGTTG PKGVM+S D +T+ + + + W K VSYLPLSH+AA +DIY+ Y V+FAD AL+GSL++TL+E RPT FFGVPRV+EK QE + + K + +A K +++ ++E + ++I K+I +V ALGL+R F S APL+ +T +F SLD+PV E G SE G S + + G+ G P K++ + + GEI +GR V MGYL+ E T+E D++G+ H+ GR+K++L+T GGENVAP PIE++IK + ++S A+VVGD K ++V LT+K + +PT L V ++LG + T E+ V +AI K I+ N++A+S A KV K+ I P + SL GE+ TL +I+ ++IE EY T
Sbjct: 185 KLGPDRLLPATSFSTTDSDGAVLIRSHDHPSSDYPTKSVYTLLKETAERAPDRPALAVKRDGVWVKWTYKEYFQDVQDIAKAFIKVGLKKSHGVGILGFNAPEWHISSLASVVAGGLSAGIYTTNSAEAVTYVLNHSRANILVLENEEQFDKVAPVWEDLGELKAIIQYIGKPTVPG--VLTWDELLEIGR--GVDDSVLQECLKKQACNQPAVLVYTSGTTGVPKGVMMSQDNLTWTVQVAQEIYQWRWDKEHAVSYLPLSHVAAQIVDIYLTAYGGATVWFADDKALQGSLINTLREARPTRFFGVPRVYEKIQEKLLDIGKQNTGFKKTIADWAKRSALEHHEERMAGRPGNSLSYRIARKVILSRVHAALGLERATLNETGGFYSSAAPLAPQTFKYFQSLDMPVLELLGSSEAGGPQTASTIGTGMRFGSVGKSYPHFETKILNPDQNGV-GEIVTRGRNVCMGYLWEEAKTNEVIDEDGWMHSGDLGKLDEDGFLFIVGRMKEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVNRKAVSKAQKVHKYMIAPTDFSLAGGELTPTLKMKRFYITEK--YEKEIERMFEYET 2161 HSP 2 Score: 334.724 bits (857), Expect = 9.230e-98 Identity = 217/649 (33.44%), Postives = 327/649 (50.39%), Query Frame = 0
Query: 626 ISSSVLITEKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYK--------KIKDSLGLDQCQEFSCGG-----APLMPTTTDFFLSLDIVIKVAYGLSECG--IFSDFAPSEYKKGTCGSIFPS-----------------TQGR-----------KKELLITAGG-------------------ENIAPILIEDNIKNFLPV-------------LSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+SS L +++ A ++TT DG+V+IR D SV + +K + + A+A+KR+G W++WTY +Y+++++ AKAFIK+GLK H V I FN PEW IS+L ++ AGG + G+Y +NSA Y++N+SR ILV+EN KV P+ E++ L +I+ G + W +L+E+G+ ++ L++ L A NQ ++YTSGTTG PKGVM+S D LT+T + E + W K VSYLPLSH+AA +DIY+ ++FAD AL+GSL+ TL+E RPT FFGVPRVYEK QE + + + +K I + K +A ++ ++ GN + Y+ ++ +LGL++ GG APL P T +F SLD+ + G SE G + + + G+ G +P T+GR K +I G + ILI +N PV LS + +GD +K ++V LT K D+ QP+ L V W SLG + T + + +V AI K I+ N VS A +V K+ + P + S+ GE+ PTLK+KR
Sbjct: 173 LSSGKLGPDRLLPATSFSTTDSDGAVLIRSHDHPSSDYPTKSVYTLLKETAERAPDRPALAVKRDGVWVKWTYKEYFQDVQDIAKAFIKVGLKKSHGVGILGFNAPEWHISSLASVVAGGLSAGIYTTNSAEAVTYVLNHSRANILVLENEEQFDKVAPVWEDLGEL--KAIIQYIGKPTVPGVLTWDELLEIGRGVDDSVLQECLKKQACNQPAVLVYTSGTTGVPKGVMMSQDNLTWTVQVAQEIYQWRWDKEHAVSYLPLSHVAAQIVDIYLTAYGGATVWFADDKALQGSLINTLREARPTRFFGVPRVYEKIQEKLLDIGKQNTGFKKTIADWAKRSALEHH---EERMAGRPGNSLSYRIARKVILSRVHAALGLERATLNETGGFYSSAAPLAPQTFKYFQSLDMPVLELLGSSEAGGPQTASTIGTGMRFGSVGKSYPHFETKILNPDQNGVGEIVTRGRNVCMGYLWEEAKTNEVIDEDGWMHSGDLGKLDEDGFLFIVGRMKEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVNRKAVSKAQKVHKYMIAPTDFSLAGGELTPTLKMKR 2104 HSP 3 Score: 91.2781 bits (225), Expect = 1.080e-17 Identity = 51/134 (38.06%), Postives = 75/134 (55.97%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ IT G EN+AP+ IED IK+ + +LS + +GD +K ++V LT K D QP+ L V W SL + T + + +V AI K I+ N+ AVS A KV K+ + P + S+ GE+ PTL
Sbjct: 1691 KEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVNRKAVSKAQKVHKYMIAPTDFSLAGGELTPTL 2092 HSP 4 Score: 41.5874 bits (96), Expect = 2.599e-2 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 0
Query: 1205 IYSFNFPEWMISNLGPVFAGGFATGLYLSNS 1235
I FN PEW IS+L V AGG + G+Y +NS
Sbjct: 470 ILGFNAPEWHISSLASVVAGGLSAGIYTTNS 562
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592925900|gb|GAXK01032524.1| (TSA: Calanus finmarchicus comp20065_c0_seq1 transcribed RNA sequence) HSP 1 Score: 429.483 bits (1103), Expect = 2.355e-130 Identity = 253/661 (38.28%), Postives = 369/661 (55.82%), Query Frame = 0
Query: 11 DKMFPNKQALASSYTTTKPDKGVHIRTESSEFL----SFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE-----NGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECG--IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTT 644
+++ P++ A+S +TT PD V IR L SV M+ N + ALA+ RN WV W Y QY + AKAFIKLGL P ++V I FN PEW IS + A+ AGGLSAG+Y TNS Y+ +SR I+VVE+ + I I + + L+ +V + G + W+ LLE+GR E L RL++ +VNQ C+L+YTSGTTGNPKGVMLS D IT+ ++ + + W+ VVSYLPLSH+A +F+DIY++++ G V+FADK AL+G+L+ TL E +PT+FFGVPRV+EK QE + E K + KV K +S +++ + +KI K++ KV Q LG +R + S APLSE +F SLD+P+QE G SE S + K G+ G PS +++ + + GEI +GR MGYL+ E T+E + +GY F GR K++++T GGENVAP PIE+ IK + +++N +V+GD K ++ LT + +E+ PT+ L V + +NLG TV +++ V I I + N++A+SNA KV K+ I P++ SL GE+ T+ F+ L T+KI+ E+ T
Sbjct: 267 ERIGPDRILPANSLSTTWPDGTVEIRFSDRHELCQAKPISVWTMLNNTVERCPNNIALAVKRNDKWVTWTYTQYLDEAITVAKAFIKLGLKPHHSVNILGFNAPEWHISAVAAVLAGGLSAGIYTTNSIDATRYVAEHSRANIMVVEDEEQYAKIEAIWERLPELQSVV--QYTGLSRSPGVESWQTLLEIGRGEDDTELKRRLANQAVNQACTLVYTSGTTGNPKGVMLSQDNITWTSQRAHEVYHWSYDSEQVVSYLPLSHVAGTFIDIYLSMWGGGTVHFADKMALQGTLLKTLVEAKPTLFFGVPRVYEKIQEKMMEIGKKNTGLKKKVGDWAKASSFEHHQATFDGQPGNSLNYKIAKKVVLSKVHQQLGFERTKGWYSSAAPLSEDVFQYFQSLDMPIQELLGSSETSGPQTASTPGAGTKLGSVGKCYPSWEVDILEPDEKGL-GEIATRGRNCCMGYLWEEGKTAELINGQGYVLSGDLGRYDEDDFLFIGGRQKEIIVTGGGENVAPVPIEDMIKAQMKDLIANCMVLGDKRKHLACILTFRTVLDENNLPTNNLHPDVIEWAENLGAESETVTDLIAEDNEEVRLEIMALIQKVNRKAVSNAQKVHKFMIAPKDFSLPGGELTPTMKIKRHFVME--LYTKKIDQMYEHET 2234 HSP 2 Score: 384.415 bits (986), Expect = 3.935e-114 Identity = 230/638 (36.05%), Postives = 346/638 (54.23%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR-RKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYK--------KIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKK-GTCGSIFPS-----------------TQGR------------------------------------------KKELLITAGGENIAPILIEDNIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
++I A +TT PDG+V IR + +L +P+SV + + + + + IA+A+KRN W+ WTY+QY E AKAFIKLGLKP H+V+I FN PEW IS + A+ AGG + G+Y +NS Y+ +SR I+VVE+ K++ I E + L VV + G W+ L+E+G+ E++ +LK+RL N A NQ C+++YTSGTTGNPKGVM+S D +T+T++ E + W+ +VSYLPLSH+A +DIY+ + G ++FADK AL+G+L++TL E +PT+FFGVPRVYEK QE + E K +K + + K ++F ++ F GN + YK K+ LG ++ + + APL +F SLD+ I+ G SE G + P K G+ G +PS T+GR +KE+++T GGEN+AP+ IED IK + +++N + +GD +K ++ LTF+ D N P+ L V EW +LG E+ TVT++I + EV I I N VSNA +V K+ + P++ S+ GE+ PT+K+KR+
Sbjct: 282 DRILPANSLSTTWPDGTVEIRFSDRHELCQAKPISVWTMLNNTVERCPNNIALAVKRNDKWVTWTYTQYLDEAITVAKAFIKLGLKPHHSVNILGFNAPEWHISAVAAVLAGGLSAGIYTTNSIDATRYVAEHSRANIMVVEDEEQYAKIEAIWERLPELQSVV--QYTGLSRSPGVESWQTLLEIGRGEDDTELKRRLANQAVNQACTLVYTSGTTGNPKGVMLSQDNITWTSQRAHEVYHWSYDSEQVVSYLPLSHVAGTFIDIYLSMWGGGTVHFADKMALQGTLLKTLVEAKPTLFFGVPRVYEKIQEKMMEIGKKNTGLKKKVGDWAKASSFEHH---QATFDGQPGNSLNYKIAKKVVLSKVHQQLGFERTKGWYSSAAPLSEDVFQYFQSLDMPIQELLGSSETSGPQTASTPGAGTKLGSVGKCYPSWEVDILEPDEKGLGEIATRGRNCCMGYLWEEGKTAELINGQGYVLSGDLGRYDEDDFLFIGGRQKEIIVTGGGENVAPVPIEDMIKAQMKDLIANCMVLGDKRKHLACILTFRTVLDENNLPTNNLHPDVIEWAENLGAESETVTDLIAEDNEEVRLEIMALIQKVNRKAVSNAQKVHKFMIAPKDFSLPGGELTPTMKIKRH 2180 HSP 3 Score: 99.3673 bits (246), Expect = 3.878e-20 Identity = 52/138 (37.68%), Postives = 85/138 (61.59%), Query Frame = 0
Query: 1310 GRKEKQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
G ++K++ +T G EN+AP+ IED IK + +++N + +GD +K ++ LTF+ D+N P+ L V +W +L E+ TVT++I + EV I I K N+ AVSNA KV K+ + P++ S+ GE+ PT+
Sbjct: 1752 GGRQKEIIVTGGGENVAPVPIEDMIKAQMKDLIANCMVLGDKRKHLACILTFRTVLDENNLPTNNLHPDVIEWAENLGAESETVTDLIAEDNEEVRLEIMALIQKVNRKAVSNAQKVHKFMIAPKDFSLPGGELTPTM 2165 HSP 4 Score: 42.3578 bits (98), Expect = 1.557e-2 Identity = 17/32 (53.12%), Postives = 21/32 (65.62%), Query Frame = 0
Query: 1204 NIYSFNFPEWMISNLGPVFAGGFATGLYLSNS 1235
NI FN PEW IS + V AGG + G+Y +NS
Sbjct: 555 NILGFNAPEWHISAVAAVLAGGLSAGIYTTNS 650
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592839075|gb|GAXK01118469.1| (TSA: Calanus finmarchicus comp124988_c0_seq1 transcribed RNA sequence) HSP 1 Score: 410.609 bits (1054), Expect = 3.213e-126 Identity = 242/606 (39.93%), Postives = 338/606 (55.78%), Query Frame = 0
Query: 12 KMFPNKQALASSYTTTKPDKGVHIRTESSEFLSFS---VPNMVKNHCLAGGSKTALAI--MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFK--TGTCGTIIPSTHAKVVKSSDDDIGG--EICIKGRCVMMGYLFNEQATSEAFDKEGY-------------FH--TGRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRA 591
KM P + + T PD V IR ESS S S VP++++ AL + + G W + YY D++ AKAFIKLGL+ F VCI N PEW I+N+G+IFAGGL+ G+YPTN + +Y++ S C ILVVE+ L + I + + +K+IV + +G + ++ WKDL++LG +L +RL + VNQCC+L++TSGTTGNPKGVMLSHD ITF K ++ ++SYLPLSH+AA+ DI+V + +G YFADK ALK SLV+ LK RPT F VPRVWEK E + +K G ++ + VK+ LGL++C F SG AP+ E+T ++FL LDI +QE YG+SE ++ +D GT G PS +++V+ D D+ G E+ + GR VMMGY+ E AT + ++G+ FH GR KDLL+T+GGENVAP PI +K LP +VS +VGD +KF+S LT+ V+ N D EPT +L+ K + G + TV ++L +M AIQ+GID AN A
Sbjct: 88 KMVPTEDLI-----TWAPDGAVRIRMESSGVGSSSPRTVPDVMRATAEESPHLPALKVKDVETGVERVWTWSSYYQDVRTVAKAFIKLGLEKFEGVCILGMNSPEWVIANVGSIFAGGLATGVYPTNGTEACKYILEQSNCSILVVEDQMQLDKVWKIREELPNIKQIV--QYNGVPTAKGVMEWKDLMQLGATVPDAKLEERLESLRVNQCCTLVFTSGTTGNPKGVMLSHDNITFTAKVGMENMKMQLKNARLLSYLPLSHVAANMFDIHVVMQAQGTTYFADKNALKSSLVENLKWCRPTEFGAVPRVWEKLMEKMLPMTK--------------------------------GKQIPSKIVKELLGLEQCHRFWSGAAPIEEKTIEYFLGLDIKIQEMYGMSETSGPHTIHMADEDNPAGTVGRTFPSFQSRLVEVGDKDMAGSKELWMWGRHVMMGYIRREDATKKDMTEDGWMKSGDLCTIDEAGFHYIVGRKKDLLITAGGENVAPQPIHENVKSQLPGIVSQVFLVGDRKKFLSCFLTLAVEVNVDTMEPTGQLSQQAKTWCRKAGSDAQTVDDVLKGPDMKIMGAIQEGIDNANNVA 1788 HSP 2 Score: 308.531 bits (789), Expect = 5.410e-90 Identity = 177/445 (39.78%), Postives = 252/445 (56.63%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIK--RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFS-DFAPSEYKKGTCGSIFPSTQGRKKELLITAGGENIA 1071
K+ ++ T PDG+V IR + + P +VP ++ + A+ +K G WT+S YY+++R AKAFIKLGL+ F V I N PEW+I+N+G+IFAGG ATG+YP+N +Y++ S C ILVVE+ L KV I E + ++ ++V + +G K + W+DL++LG T + +L++RL ++ NQCC++++TSGTTGNPKGVM+SHD +T+TAK +E K ++SYLPLSH+AAN DI+VV+ +G YFADK+ALK SLV LK RPT F VPRV+EK E K G Q+ K +K+ LGL+QC F G AP+ T ++FL LDI I+ YG+SE G + A + GT G FPS Q R L+ G +++A
Sbjct: 88 KMVPTEDLITWAPDGAVRIRMESSGVGSSSPRTVPDVMRATAEESPHLPALKVKDVETGVERVWTWSSYYQDVRTVAKAFIKLGLEKFEGVCILGMNSPEWVIANVGSIFAGGLATGVYPTNGTEACKYILEQSNCSILVVEDQMQLDKVWKIREELPNIKQIV--QYNGVPTAKGVMEWKDLMQLGATVPDAKLEERLESLRVNQCCTLVFTSGTTGNPKGVMLSHDNITFTAKVGMENMKMQLKNARLLSYLPLSHVAANMFDIHVVMQAQGTTYFADKNALKSSLVENLKWCRPTEFGAVPRVWEKLME--------------------------------KMLPMTKGKQIPSKIVKELLGLEQCHRFWSGAAPIEEKTIEYFLGLDIKIQEMYGMSETSGPHTIHMADEDNPAGTVGRTFPSFQSR----LVEVGDKDMA 1308 HSP 3 Score: 103.605 bits (257), Expect = 1.184e-21 Identity = 54/110 (49.09%), Postives = 76/110 (69.09%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLP-VLSNVVAIGDLKKFISVFLTF--KVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEI 1162
GRKK+LLITAGGEN+AP I +N+K+ LP ++S V +GD KKF+S FLT +VN DT +P+ +LS K WC G +A TV ++++ ++M AIQ+GID AN +
Sbjct: 1459 GRKKDLLITAGGENVAPQPIHENVKSQLPGIVSQVFLVGDRKKFLSCFLTLAVEVNVDTM-EPTGQLSQQAKTWCRKAGSDAQTVDDVLKGPDMKIMGAIQEGIDNANNV 1785 HSP 4 Score: 91.6633 bits (226), Expect = 7.188e-18 Identity = 50/120 (41.67%), Postives = 75/120 (62.50%), Query Frame = 0
Query: 1301 FRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIA 1418
F Y+V GRK K L ITAG EN+AP I + +K+ LP ++S V +GD KKF+S FLT V + + +P+ +LS K WC +A TV ++++ ++++ AIQ+GID AN +A
Sbjct: 1444 FHYIV----GRK-KDLLITAGGENVAPQPIHENVKSQLPGIVSQVFLVGDRKKFLSCFLTLAVEVNVDTMEPTGQLSQQAKTWCRKAGSDAQTVDDVLKGPDMKIMGAIQEGIDNANNVA 1788 HSP 5 Score: 47.7506 bits (112), Expect = 2.810e-4 Identity = 20/39 (51.28%), Postives = 28/39 (71.79%), Query Frame = 0
Query: 1205 IYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIY 1243
I N PEW+I+N+G +FAGG ATG+Y +N T ++C Y
Sbjct: 364 ILGMNSPEWVIANVGSIFAGGLATGVYPTNGT--EACKY 474
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592756206|gb|GAXK01198207.1| (TSA: Calanus finmarchicus comp338023_c0_seq2 transcribed RNA sequence) HSP 1 Score: 404.445 bits (1038), Expect = 5.115e-123 Identity = 232/611 (37.97%), Postives = 348/611 (56.96%), Query Frame = 0
Query: 12 KMFPNKQALASSYTTTKPDKGVHIRTE---SSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE-----NGDQKVIHKIGDKLIFQKVKQALGLDRCL-----EFISGGAPLSERTRDFFLSLDIPVQECYGLSECG--IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRA 591
K+ P++ A+S++TT D V IR+ SS++ + SV ++K + ALA+ R+G WVKW Y +Y+ D++ AKAFIK+GL + V I FN PEW IS+L ++ AGGLSAG+Y TNS+ Y++N+SR ILV+EN + ++ + +++ LK I+ PG ++ W +LLE+GR L + L + NQ L+YTSGTTG PKGVM+S D +T+ + + + W K VSYLPLSH+AA +DIY+ Y V+FAD AL+GSL++TL+E RPT FFGVPRV+EK QE + + K + +A K +++ ++E + ++I K+I +V ALGL+R F S APL+ +T +F SLD+PV E G SE G S + + G+ G P K++ + + GEI +GR V MGYL+ E T+E D++G+ H+ GR+K++L+T GGENVAP PIE++IK + ++S A+VVGD K ++V LT+K + +PT L V ++LG + T E+ V +AI K I+ N++A
Sbjct: 185 KLGPDRLLPATSFSTTDSDGAVLIRSHDHPSSDYPTKSVYTLLKETAERAPDRPALAVKRDGVWVKWTYKEYFQDVQDIAKAFIKVGLKKSHGVGILGFNAPEWHISSLASVVAGGLSAGIYTTNSAEAVTYVLNHSRANILVLENEEQFDKVAPVWEDLGELKAIIQYIGKPTVPG--VLTWDELLEIGRGVDDSVLQECLKKQACNQPAVLVYTSGTTGVPKGVMMSQDNLTWTVQVAQEIYQWRWDKEHAVSYLPLSHVAAQIVDIYLTAYGGATVWFADDKALQGSLINTLREARPTRFFGVPRVYEKIQEKLLDIGKQNTGFKKTIADWAKRSALEHHEERMAGRPGNSLSYRIARKVILSRVHAALGLERATLNETGGFYSSAAPLAPQTFKYFQSLDMPVLELLGSSEAGGPQTASTIGTGMRFGSVGKSYPHFETKILNPDQNGV-GEIVTRGRNVCMGYLWEEAKTNEVIDEDGWMHSGDLGKLDEDGFLFIVGRMKEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVNRKA 2008 HSP 2 Score: 305.834 bits (782), Expect = 3.202e-88 Identity = 202/614 (32.90%), Postives = 305/614 (49.67%), Query Frame = 0
Query: 626 ISSSVLITEKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT-KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYK--------KIKDSLGLDQCQEFSCGG-----APLMPTTTDFFLSLDIVIKVAYGLSECG--IFSDFAPSEYKKGTCGSIFPS-----------------TQGR-----------KKELLITAGG-------------------ENIAPILIEDNIKNFLPV-------------LSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMAN 1160
+SS L +++ A ++TT DG+V+IR D SV + +K + + A+A+KR+G W++WTY +Y+++++ AKAFIK+GLK H V I FN PEW IS+L ++ AGG + G+Y +NSA Y++N+SR ILV+EN KV P+ E++ L +I+ G + W +L+E+G+ ++ L++ L A NQ ++YTSGTTG PKGVM+S D LT+T + E + W K VSYLPLSH+AA +DIY+ ++FAD AL+GSL+ TL+E RPT FFGVPRVYEK QE + + + +K I + K +A ++ ++ GN + Y+ ++ +LGL++ GG APL P T +F SLD+ + G SE G + + + G+ G +P T+GR K +I G + ILI +N PV LS + +GD +K ++V LT K D+ QP+ L V W SLG + T + + +V AI K I+ N
Sbjct: 173 LSSGKLGPDRLLPATSFSTTDSDGAVLIRSHDHPSSDYPTKSVYTLLKETAERAPDRPALAVKRDGVWVKWTYKEYFQDVQDIAKAFIKVGLKKSHGVGILGFNAPEWHISSLASVVAGGLSAGIYTTNSAEAVTYVLNHSRANILVLENEEQFDKVAPVWEDLGEL--KAIIQYIGKPTVPGVLTWDELLEIGRGVDDSVLQECLKKQACNQPAVLVYTSGTTGVPKGVMMSQDNLTWTVQVAQEIYQWRWDKEHAVSYLPLSHVAAQIVDIYLTAYGGATVWFADDKALQGSLINTLREARPTRFFGVPRVYEKIQEKLLDIGKQNTGFKKTIADWAKRSALEHH---EERMAGRPGNSLSYRIARKVILSRVHAALGLERATLNETGGFYSSAAPLAPQTFKYFQSLDMPVLELLGSSEAGGPQTASTIGTGMRFGSVGKSYPHFETKILNPDQNGVGEIVTRGRNVCMGYLWEEAKTNEVIDEDGWMHSGDLGKLDEDGFLFIVGRMKEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVN 1999 HSP 3 Score: 66.6254 bits (161), Expect = 4.897e-10 Identity = 38/106 (35.85%), Postives = 57/106 (53.77%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIA 1418
K++ IT G EN+AP+ IED IK+ + +LS + +GD +K ++V LT K D QP+ L V W SL + T + + +V AI K I+ N+ A
Sbjct: 1691 KEILITGGGENVAPVPIEDSIKDEMSEILSQAMVVGDKRKHLAVILTLKTVLDSKNQPTDNLHPDVTAWLESLGSSSLTPKEVAAEDNDDVKEAIMKSINTVNRKA 2008 HSP 4 Score: 41.5874 bits (96), Expect = 2.414e-2 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 0
Query: 1205 IYSFNFPEWMISNLGPVFAGGFATGLYLSNS 1235
I FN PEW IS+L V AGG + G+Y +NS
Sbjct: 470 ILGFNAPEWHISSLASVVAGGLSAGIYTTNS 562
BLAST of EMLSAG00000003336 vs. C. finmarchicus
Match: gi|592751451|gb|GAXK01202962.1| (TSA: Calanus finmarchicus comp342936_c4_seq1 transcribed RNA sequence) HSP 1 Score: 385.185 bits (988), Expect = 6.564e-116 Identity = 230/618 (37.22%), Postives = 340/618 (55.02%), Query Frame = 0
Query: 15 PNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMR-NGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE-----NGDQKVIHKIGDKLIFQKVKQALGLDRCLEF--ISGGAPLSERTRDFFLSLDIPVQECYGLSECG----IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCN--YTTVGEILYHKPPG-VMKAIQKGIDEANKRAISNAAKV 598
P++ A + P+ V +R + S S SVP + K + AL G +V W + QYY D++ AK FI LGL PF+TV I + PEW +SNLGAI AGG + G+Y TNS + EY+ +SR I+VV + L I I N+ LK IV+ E PG ++ W +LL++G+ L++ ++ I VNQCC L YTSGTTGNPKG MLSHD IT+ + + W V+SYLP SHIA +D+Y+ + G YFADK ALKG+L++ LK +P+ GV RV+EK +E +K Q + K + K+ K ++H+++ V +++ KL+F KV ALGL + I GGA +S T +FLSLDI + E ++E I V DF+ G GT + + D++ GE+ +GR + MGYL NE+ T EA +G+FH+ GR+K++L+T+GGENVAP IE+ IK+ LP +VSN +VVGD +K+++ +T+KV+ + ++ + + D+ C T+ E+L K G V + I G++ AN +A+SN A+V
Sbjct: 3 PDQILPADDFRAWLPNSAVKLRMKDSGPQSAPPISVPTLFKTQVENLPNNLALRTRDPTGEYVDWTWTQYYDDVRTVAKGFISLGLQPFHTVAILGHHSPEWHMSNLGAIHAGGFATGIYQTNSPTACEYIAQDSRANIIVVGDQIQLEKILSIRDNLPHLKAIVLYEGTSDVPG--VVSWNELLKIGKDNIDTTLDETINGIGVNQCCILCYTSGTTGNPKGTMLSHDSITYAAIRNTEHYGWQYGTESVMSYLPQSHIAGMMMDVYLIMSKGGTCYFADKNALKGTLIENLKLYQPSKMMGVTRVFEKVEEAMKMQGASSKGLKKKIGDWAKSQALHHHQMETSGKSHNSVGYQLARKLVFSKVHAALGLGNAAPYGIIIGGAAVSPETVRYFLSLDIKLLEIISMTEASACVQIGNRVTPGDFRIGKVGTPYQDQLEVKLINEDENGAGEMLTRGRAMCMGYLNNEEKTKEAMGDDGWFHSGDLCKMDAEGFYTVVGRIKEILITAGGENVAPTNIEDEIKNALPNIVSNVMVVGDKQKYLTCLITLKVQIDPHTMAPTDKLEPLAADWAKSVCGEEIGTLTELLSGKGWGKVQEEIDAGVERANDKAVSNVARV 1850 HSP 2 Score: 317.775 bits (813), Expect = 2.739e-92 Identity = 206/633 (32.54%), Postives = 328/633 (51.82%), Query Frame = 0
Query: 625 FISSSVLI--TEKIEDAKEYTTTLPDGSVIIRRKKDDLQ---PMSVPSFVKHNCLKGGSQIAMAIKR-NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKI-SIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFS--CGGAPLMPTTTDFFLSLDIVIKVAYGLSECG----IFSDFAPSEYKKGTCGSIFPS------------------TQGR--------KKELLITAGGEN----------------------IAPILIEDNIKNFLP-------------VLSNVVAIGDLKKFISVFLTFKVNSDTNGQ-PSTELSNSVKEWCHSL-GYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARV 1170
F+SSS+ ++I A ++ LP+ +V +R K Q P+SVP+ K + +A+ + G+++ WT++QYY ++R AK FI LGL+PFHTV+I + PEW +SNLGAI AGGFATG+Y +NS + EY+ +SR I+VV + L+K+ I +N+ HL +V+ E + +S W +L+++GK + L + ++ I NQCC + YTSGTTGNPKG M+SHD +TY A E +GW S++SYLP SHIA +D+Y+++ G YFADK+ALKG+L+ LK +P+ GV RV+EK +E G+++KI KSQ + ++ + +N+ G + L ++++ K+ +LGL + GGA + P T +FLSLDI + ++E I + P +++ G G+ + T+GR +E A G++ I ILI +N P ++SNV+ +GD +K+++ +T KV D + P+ +L +W S+ G E T+T ++ + +V I G++ AN+ VSN ARV
Sbjct: 3 FVSSSLQQRGPDQILPADDFRAWLPNSAVKLRMKDSGPQSAPPISVPTLFKTQVENLPNNLALRTRDPTGEYVDWTWTQYYDDVRTVAKGFISLGLQPFHTVAILGHHSPEWHMSNLGAIHAGGFATGIYQTNSPTACEYIAQDSRANIIVVGDQIQLEKILSIRDNLPHLKAIVLYEGTSDVPGVVS--WNELLKIGKDNIDTTLDETINGIGVNQCCILCYTSGTTGNPKGTMLSHDSITYAAIRNTEHYGWQYGTESVMSYLPQSHIAGMMMDVYLIMSKGGTCYFADKNALKGTLIENLKLYQPSKMMGVTRVFEKVEEAMKMQGASSKGLKKKIGDWAKSQALHHHQMETSGKSHNSVGYQ-----LARKLVFSKVHAALGLGNAAPYGIIIGGAAVSPETVRYFLSLDIKLLEIISMTEASACVQIGNRVTPGDFRIGKVGTPYQDQLEVKLINEDENGAGEMLTRGRAMCMGYLNNEEKTKEAMGDDGWFHSGDLCKMDAEGFYTVVGRIKEILITAGGENVAPTNIEDEIKNALPNIVSNVMVVGDKQKYLTCLITLKVQIDPHTMAPTDKLEPLAADWAKSVCGEEIGTLTELLSGKGWGKVQEEIDAGVERANDKAVSNVARV 1880 HSP 3 Score: 82.4185 bits (202), Expect = 6.052e-15 Identity = 48/116 (41.38%), Postives = 73/116 (62.93%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDKNGQ-PSTELSNLVKDWCHSL-SYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKV 1425
K++ ITAG EN+AP IED+IKN LP ++SNV+ +GD +K+++ +T KV D + P+ +L L DW S+ E T+T ++ K +V I G+++AN AVSN A+V
Sbjct: 3 KEILITAGGENVAPTNIEDEIKNALPNIVSNVMVVGDKQKYLTCLITLKVQIDPHTMAPTDKLEPLAADWAKSVCGEEIGTLTELLSGKGWGKVQEEIDAGVERANDKAVSNVARV 350 HSP 4 Score: 45.0542 bits (105), Expect = 1.896e-3 Identity = 20/39 (51.28%), Postives = 27/39 (69.23%), Query Frame = 0
Query: 1205 IYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIY 1243
I + PEW +SNLG + AGGFATG+Y +NS + +C Y
Sbjct: 1461 ILGHHSPEWHMSNLGAIHAGGFATGIYQTNSPT--ACEY 1571
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000003336 (pep:novel supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28") HSP 1 Score: 2985.67 bits (7739), Expect = 0.000e+0 Identity = 1447/1447 (100.00%), Postives = 1447/1447 (100.00%), Query Frame = 0
Query: 1 MEAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNIYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIYRRTWINTGETINPMEGRTENRKGVIKSHNMLTYAAESAXEKHVRTKKISIVRNCSQCFRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTLN 1447
MEAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNIYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIYRRTWINTGETINPMEGRTENRKGVIKSHNMLTYAAESAXEKHVRTKKISIVRNCSQCFRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTLN
Sbjct: 1 MEAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNIYSFNFPEWMISNLGPVFAGGFATGLYLSNSTSLKSCIYRRTWINTGETINPMEGRTENRKGVIKSHNMLTYAAESAXEKHVRTKKISIVRNCSQCFRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTLN 1447
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000000839 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1141:106424:108541:-1 gene:EMLSAG00000000839 transcript:EMLSAT00000000839 description:"maker-LSalAtl2s1141-augustus-gene-0.8") HSP 1 Score: 444.121 bits (1141), Expect = 2.271e-139 Identity = 248/607 (40.86%), Postives = 354/607 (58.32%), Query Frame = 0
Query: 623 VGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQ-RLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKIS-IVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT------GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPS------------------------TQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDT-NGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+ + S + I+DA + T GS + + P+S+P ++ + G A+ ++RNGD + WT+ Y +++R AAKA +K GL+ + V I FN PEW I++LGAI+AGG +G+YP+N+ +Y+ ++ C I+ VEN LQK+Q I + HL K +I+ G D + W I LG E + L + L I AN+CC ++YTSGTTGNPKGVM+SHD +T+TAK E + +K VSYLPLSHIAA +DI++ I + +YFADK+ALKG+L TL+E PT+FFGVPRVYEK QE I K +K I K + YN + +K + N+++++K+K LG D+C F GAPL T ++F+SLDI+I YG+SE CG +K G G P GR+KEL+ITAGGENI P+LIED +K LP+LSN + IGD KK++S FLT KVN D+ +PS LS +EWC+ LG TV+ I+ + +V++AIQ+GI+ N SNA +++KW +IP++ S+ GE+GPTLK+KR
Sbjct: 5 ISLVPSDRFVVSSIDDAVKLRT----GSEV----PSNYDPISIPQLLEETASEAGYIPALRVRRNGDLITWTFRDYLKDVRTAAKAMLKCGLEEGNGVCILGFNSPEWFIADLGAIYAGGIPSGIYPTNNPDSCKYIASHCHCTIMFVENQEQLQKIQSIKTQLPHLRK--IIQYIGEPTDPDVLSWNAFISLGTEPELEXLLEISLSKIXANKCCHLVYTSGTTGNPKGVMLSHDNITFTAKNTGEHYKLKEKEERFVSYLPLSHIAAVIVDIFIPITFKVTVYFADKNALKGTLTETLRECHPTLFFGVPRVYEKMQEKIMGASKSTKGLKKHIGRWAKKTGYRYNKSLITHPNQDKDIQYKVANKVVFQKVKALLGFDKCVHFVSSGAPLSIETLEYFMSLDILIMELYGMSECCGPHXANTLRAWKPGKVGRDLPGFLTKIDKDGEIHMKGELDSSGFLRVTGRRKELIITAGGENIPPVLIEDKLKASLPILSNAMVIGDNKKYLSCFLTLKVNEDSETHEPSDRLSPLSEEWCNKLGSHVRTVSEILENPEEQVLAAIQEGINKYNINATSNAQKIQKWIIIPKDFSVSTGELGPTLKMKR 601 HSP 2 Score: 436.802 bits (1122), Expect = 1.260e-136 Identity = 247/610 (40.49%), Postives = 361/610 (59.18%), Query Frame = 0
Query: 30 DKGVHIRTES---SEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLND-RLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY------YENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH-TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKV-KSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFI 626
D V +RT S S + S+P +++ G AL + RNG+ + W + Y D++ AKA +K GL+ VCI FN PEWFI++LGAI+AGG+ +G+YPTN+ + +Y+ ++ C I+ VEN + L+ I I + L+KI I+ G +++ W + LG + ++ L + LS I N+CC L+YTSGTTGNPKGVMLSHD ITF K + + + VSYLPLSHIAA +DI++ + K VYFADK ALKG+L +TL+E PT+FFGVPRV+EK QE I SK+ K + + + K Y + N D+ + +K+ +K++FQKVK LG D+C+ F+S GAPLS T ++F+SLDI + E YG+SE CG + +K G G +P K+ D GEI +KG D G+ TGR K+L++T+GGEN+ P IE+ +K LP++SNA+V+GD +K++S LT+KV + +E EP+ L+ ++ LG + TV EIL + V+ AIQ+GI++ N A SNA K++KW I+P++ S+ GE+G TL FI
Sbjct: 19 DDAVKLRTGSEVPSNYDPISIPQLLEETASEAGYIPALRVRRNGDLITWTFRDYLKDVRTAAKAMLKCGLEEGNGVCILGFNSPEWFIADLGAIYAGGIPSGIYPTNNPDSCKYIASHCHCTIMFVENQEQLQKIQSIKTQLPHLRKI--IQYIGEPTDPDVLSWNAFISLGTEPELEXLLEISLSKIXANKCCHLVYTSGTTGNPKGVMLSHDNITFTAKNTGEHYKLKEKEERFVSYLPLSHIAAVIVDIFIPITFKVTVYFADKNALKGTLTETLRECHPTLFFGVPRVYEKMQEKIMGASKSTKGLKKHIGRWAKKTGYRYNKSLITHPNQDKDIQYKVANKVVFQKVKALLGFDKCVHFVSSGAPLSIETLEYFMSLDILIMELYGMSECCGPHXANTLRAWKPGKVGRDLPGFLTKI------DKDGEIHMKGE----------------LDSSGFLRVTGRRKELIITAGGENIPPVLIEDKLKASLPILSNAMVIGDNKKYLSCFLTLKVNEDSETHEPSDRLSPLSEEWCNKLGSHVRTVSEILENPEEQVLAAIQEGINKYNINATSNAQKIQKWIIIPKDFSVSTGELGPTLKMKRYFI 604 HSP 3 Score: 141.354 bits (355), Expect = 9.394e-35 Identity = 71/138 (51.45%), Postives = 99/138 (71.74%), Query Frame = 0
Query: 1310 GRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
GR+ K+L ITAG ENI P+LIEDK+K LP+LSN + IGD KK++S FLT KVN D + +PS LS L ++WC+ L TV+ I+ + +V++AIQ+GI+K N A SNA K++KW +IP++ S+ GE+GPTL
Sbjct: 461 GRR-KELIITAGGENIPPVLIEDKLKASLPILSNAMVIGDNKKYLSCFLTLKVNEDSETHEPSDRLSPLSEEWCNKLGSHVRTVSEILENPEEQVLAAIQEGINKYNINATSNAQKIQKWIIIPKDFSVSTGELGPTL 597
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000006219 (pep:novel supercontig:LSalAtl2s:LSalAtl2s33:201552:203709:-1 gene:EMLSAG00000006219 transcript:EMLSAT00000006219 description:"maker-LSalAtl2s33-augustus-gene-2.8") HSP 1 Score: 347.436 bits (890), Expect = 1.900e-106 Identity = 190/463 (41.04%), Postives = 284/463 (61.34%), Query Frame = 0
Query: 15 PNKQALASSYTTTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIE-EDGFRPGENLILWKDLLELGR---KEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKA----IVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYE---NGDQK--VIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMG 463
P++ A+SYT + P+ V +R+ L SVP ++KN + A+A+ R +W KW Y +YY + +L A+ FI+LGL+PF++V I FN PEW +S +GAIFAGG AG+Y TNS+ +Y+ N+ R Q+LVVE+ + L+ ++ I + +T LK I+V ++ F G+N+ W++ ++L R K+G + + +RL +IS+NQCCSLIYTSGTTG PKGVM+SHD +T+ + + KH VSYLPLSH AA +DI++ + +YF D+ ALKGSLV TL+EVRP FFGVPRV+EK +E ++E K + + K +++ G Q + + + KLIF+KVK+ LGLD+ G AP+S +T ++FLSLDIP+ +CYG+SE G + + + T G + K+ + D++ GEIC+ R VMMG
Sbjct: 4 PDRLLDANSYTNSDPNGAVKLRSSPDSILPLSVPTLLKNASDSYPKSVAMAVKRADHWNKWTYKEYYEEAQLAAEGFIELGLEPFHSVGILGFNAPEWSLSLVGAIFAGGFGAGIYATNSAEAVKYVANDCRVQVLVVEDSKQLQKVNAIKEQLTFLKAIIVYGYQEKFEGGDNIYSWREFMDLARNAQKKG-ENVQNRLENISINQCCSLIYTSGTTGPPKGVMMSHDNVTWTARTSNHYLGLKHGDRG-----VSYLPLSHSAAQMMDIWMPIAQGMSMYFCDRNALKGSLVSTLREVRPAYFFGVPRVYEKIEEKMRELGKANTGFKKAFGEWAKKTGLNHNMKSLEGQQPGGISYPLAKKLIFKKVKEGLGLDKARMCGIGAAPVSRKTLEYFLSLDIPLYDCYGMSESSGPQTGNRPGNHRFMTVGGNVGGCVTKIYE-PDENGEGEICMSSRNVMMG 459 HSP 2 Score: 340.887 bits (873), Expect = 4.450e-104 Identity = 178/409 (43.52%), Postives = 255/409 (62.35%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE-EDGFIADKLSILWRDLIELGKTEEE--DQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN---------LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE 1030
+++ DA YT + P+G+V +R D + P+SVP+ +K+ +AMA+KR W +WTY +YY E +LAA+ FI+LGL+PFH+V I FN PEW +S +GAIFAGGF G+Y +NSA +Y+ N+ R Q+LVVE++ LQKV I E + L ++V ++ F WR+ ++L + ++ + ++ RL NI+ NQCCS+IYTSGTTG PKGVM+SHD +T+TA+T G VSYLPLSH AA +DI++ I +YF D++ALKGSLV TL+EVRP FFGVPRVYEK IEEK+ +L K+ + A N K ++ L ++++KK+K+ LGLD+ + G AP+ T ++FLSLDI + YG+SE
Sbjct: 5 DRLLDANSYTNSDPNGAVKLRSSPDSILPLSVPTLLKNASDSYPKSVAMAVKRADHWNKWTYKEYYEEAQLAAEGFIELGLEPFHSVGILGFNAPEWSLSLVGAIFAGGFGAGIYATNSAEAVKYVANDCRVQVLVVEDSKQLQKVNAIKEQLTFLKAIIVYGYQEKFEGGDNIYSWREFMDLARNAQKKGENVQNRLENISINQCCSLIYTSGTTGPPKGVMMSHDNVTWTARTSNHYLGLKHGDRGVSYLPLSHSAAQMMDIWMPIAQGMSMYFCDRNALKGSLVSTLREVRPAYFFGVPRVYEK----IEEKMRELGKANTGFKKAFGEWAKKTGLNHNMKSLEGQQPGGISYPLAKKLIFKKVKEGLGLDKARMCGIGAAPVSRKTLEYFLSLDIPLYDCYGMSE 409
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000010718 (pep:novel supercontig:LSalAtl2s:LSalAtl2s70:458722:474012:1 gene:EMLSAG00000010718 transcript:EMLSAT00000010718 description:"augustus_masked-LSalAtl2s70-processed-gene-4.2") HSP 1 Score: 222.631 bits (566), Expect = 8.349e-62 Identity = 147/460 (31.96%), Postives = 233/460 (50.65%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIAD---------KLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKT---VVEKHGWTKKIS-----IVSYLPLSHIAANALDIYVVICCRGELYFADKSAL---KGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCY---NTTGNKKFIK----------NLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEY-KKGTCGSIFPSTQ 1055
++I A+E+++T +G V +R+ + L +P+SVP+ +++ +A IKR+ +W+ WTY QY +E+ AK+FI GL +V+I N PEW++S++ AIF+GG A GL S + + + ++VV+N L+K+ I + L ++ I+ D ++D K+ + W ++I LG +D+L+ RL +A NQ C+IIYTSG PKGVMISHD LT+ AKT ++ G+ + + ++S LPLS I+A +DIY +C G L + L + T+ +V+PTV F +YE+ I K+ LK+S I I+ D + C +K IK L + KK K+ LG F GAP+ F DIV+ +G +E CG+ + P Y K G+ G P +
Sbjct: 42 DQILPAEEFSSTRRNGCVKLRKVEHGLGSEEPISVPTLLQNIAENYPDVVAFKIKRDEEWISWTYEQYLKEVNTVAKSFIXXGLHRHXSVAILGNNSPEWIVSDVAAIFSGGIAVGLNKWLSPDEIAKICIDCKADVIVVQNEAFLKKILLIQHKLPELRTIIQIDGDPPLSDKRRLHRTHKKIILSWEEMIVLGHNLTDDRLRDRLKKVAINQVCTIIYTSGANETPKGVMISHDNLTWCAKTSMGLIRAPGFNRMPAPGEEILISVLPLSSISAQIIDIYYTLCVVGTLCISSNKNLLTQGDAFFDTVFDVQPTVLFAPTLIYER----IFHKLRNLKRSMSGIQKIVLDWSSCTLRDKHLNEQKKIKATRKLNQWQTALAKNTVTKKYKEMLGFTSRTVFFSRGAPMAREVLRFLSGYDIVVHETFGQTENCGLLTANIPKRYCKIGSTGRPVPGVK 497 HSP 2 Score: 189.889 bits (481), Expect = 8.603e-51 Identity = 135/476 (28.36%), Postives = 228/476 (47.90%), Query Frame = 0
Query: 7 NSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDG--------FRPGENLIL-WKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTK---AIVKKHDW----TTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYAL---KGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDAS------MHYYENGDQKVIHK-------IGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCS-VQKSDFKTGTCGTIIPSTHAKVVKSS 445
NS + P++ A +++T+ + V +R S SVP +++N A I R+ W+ W Y+QY ++ AK+FI GL +V I N PEW +S++ AIF+GG++ GL S + + + ++VV+N L+ I I + L+ I+ I+ D R + +IL W++++ LG DRL DRL +++NQ C++IYTSG PKGVM+SHD +T+ K +++ + + I++S LPLS I+A +DIY L + G + + L + DT+ +V+PT+ F ++E+ I + +N K+S + ++ D S H E K K + + +K K+ LG F S GAP++ F DI V E +G +E CG+ + + K K G+ G +P + +++
Sbjct: 33 NSNSQLNGPDQILPAEEFSSTRRNGCVKLRKVEHGLGSEEPISVPTLLQNIAENYPDVVAFKIKRDEEWISWTYEQYLKEVNTVAKSFIXXGLHRHXSVAILGNNSPEWIVSDVAAIFSGGIAVGLNKWLSPDEIAKICIDCKADVIVVQNEAFLKKILLIQHKLPELRTIIQIDGDPPLSDKRRLHRTHKKIILSWEEMIVLGHNLTDDRLRDRLKKVAINQVCTIIYTSGANETPKGVMISHDNLTWCAKTSMGLIRAPGFNRMPAPGEEILISVLPLSSISAQIIDIYYTLCVVGTLCISSNKNLLTQGDAFFDTVFDVQPTVLFAPTLIYER----IFHKLRNLKRSMSGIQKIVLDWSSCTLRDKHLNEQKKIKATRKLNQWQTALAKNTVTKKYKEMLGFTSRTVFFSRGAPMAREVLRFLSGYDIVVHETFGQTENCGLLTANIPKRYCKIGSTGRPVPGVKMNIEQTT 504
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000010073 (pep:novel supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10") HSP 1 Score: 124.405 bits (311), Expect = 5.509e-29 Identity = 131/509 (25.74%), Postives = 218/509 (42.83%), Query Frame = 0
Query: 88 AKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEED-------GFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHD--IITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVY-----FADKYA--LKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFF-LSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDI--------GGEICIKGRCVMMGYLFNEQATSEAFDKE----GYFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSV 551
K +LGL+ +CI++ + EW S LG LY + +N ++ L+ + +L + ++L+ ++K I+ E+ G++ G ++ +++++ELG K + + + + ++YTSG+TG PKGV+L H+ I T +V K ++ YLPL+HI ++ + ++ Y DK +KGS D +RPT+ VP V ++ +GI E + K + HY G + I + LIF+ ++ +G + +SGGAPLS D+ +LDIP+ + YGL+E C + + + TG G +++ D GEI I G + GY Q T+E F K+ +F TG R KDL+ GE V+ +E IK P+V N GD K V L + D +EL +S+
Sbjct: 156 GKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTH-DLLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVIELGSKS-----ETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGTIAAFHLVVKDKIPEETYL--GYLPLAHIYELLAEMTMLIHGIKVGYSSPNTMIDKSTKVMKGSKGDC-SVLRPTLMCAVPLVIDRIYKGIHENLSKKSKFMRSLLHFCYHYKSHYRRLGMRTPIM---NALIFRPMRAIVG-GKVRVIVSGGAPLSHNIHDYVSCALDIPIHQGYGLTESCAGGSVMHREELSTGRVGPPGQGVLIRLINWEDGGYRVTDKPRPRGEIVIGGPNIAHGYYKLPQKTAEDFYKDENNVRWFKTGDVGEFAEDGTLKIIDRKKDLVKLQYGEYVSLGKVETVIKT-CPLVENVCAYGDPTKSYCVALVVP-----DRNKLTELGESI 645 HSP 2 Score: 89.3521 bits (220), Expect = 3.742e-18 Identity = 104/375 (27.73%), Postives = 172/375 (45.87%), Query Frame = 0
Query: 706 AKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADK------LSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDML--TYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELY-----FADKSA--LKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLG------NQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFF-LSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQG 1056
K +LGL+ + I++ + EWM S LG LY + + +N ++ L+ ++ +L K++ +LE + ++ E+ D+ + IL ++++IELG E + A I+YTSG+TG PKGV++ H+ L T A +V K ++ + + YLPL+HI ++ ++I Y DKS +KGS +RPT+ VP V ++ +GI E +S K KF+ ++L FCY+ K + LG N ++++ ++ +G + + GGAPL D+ +LDI I YGL+E C S E G G P QG
Sbjct: 156 GKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTHD-LLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVIELGSKSETKATPPTKDDPAI-----IMYTSGSTGVPKGVILPHESLIGTIAAFHLVVKDKIPEE-TYLGYLPLAHIYELLAEMTMLIHGIKVGYSSPNTMIDKSTKVMKGS-KGDCSVLRPTLMCAVPLVIDRIYKGIHENLS---KKSKFMRSLLH---FCYHY---KSHYRRLGMRTPIMNALIFRPMRAIVG-GKVRVIVSGGAPLSHNIHDYVSCALDIPIHQGYGLTESCAGGSVMHREELSTGRVG---PPGQG 509
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000011396 (pep:novel supercontig:LSalAtl2s:LSalAtl2s787:62044:87799:1 gene:EMLSAG00000011396 transcript:EMLSAT00000011396 description:"maker-LSalAtl2s787-augustus-gene-0.76") HSP 1 Score: 118.242 bits (295), Expect = 1.035e-26 Identity = 128/519 (24.66%), Postives = 225/519 (43.35%), Query Frame = 0
Query: 70 GNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGE---------NLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYAL--KGSLVD-----TLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENG-DQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFF-LSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAFDKEG---YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEK--FISVXLTIKVKSNEDG 541
G++ +Y+ A + LG++P + I S + EW +S A LY + E +N + +++V + +VL IL+ +K +VVIE + + I+++DLL+ K+ +R S S N ++YTSG+TG PKGVM++H ++++ D+TT+ ++YLPL+HI ++ LY ++ ++ L GS V +PT+ VP + ++ +GI + + + + ++ F + + G D +++K +F+ V+ LG R ++GGAP+S T F +L +PV + YGL+E G + DF T G + K+V + GEI G + GY N + T++ F +E +F TG R KDL+ SGGE ++ +E+ +K P + N D K ++ + K N+ G
Sbjct: 1942 GDYSYISYNGLRRQTSDLADGLMDLGIEPRSRIAILSESRQEWVVSMYAAFKNNYTLVTLYTNLGNDAVEEGLNETEVPLVIVSD-EVLPKFKGILEACPLIKYVVVIEAGNGKKSDLSVNYQNNFQFIMYRDLLKRRSKKKYNR-----SIPSTNDTAIIMYTSGSTGRPKGVMVNHGDAIAGLRSVISYTDFTTSDR-YLAYLPLAHIMEIVAEM-ACLYCGVKIGYSSPLTLTDNGSKVKKGCKGDANVFKPTLMCAVPLLLQRIYKGIIDNMRKKGWAMEELFNYFVAYKIKWQXRGFDXPLLNKT----LFRTVRYFLG-GRVRLLLNGGAPISHETHLFTRTTLSVPVLQAYGLTELSGAAAISNEYDFSTCRVGPPLIGVKVKLVNWEEGGYTINDKPFPRGEIHCGGESLCQGYFKNPEKTADDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILK-TCPFIENICTYADPLKDYLFAIAIPDKAHVNQRG 2446 HSP 2 Score: 92.0485 bits (227), Expect = 1.111e-18 Identity = 95/376 (25.27%), Postives = 165/376 (43.88%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLS---------ILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVIC-----CRGELYFADK-SALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFF-LSLDIVIKVAYGLSECGIFSDFAPSEYKKGTC 1047
GD+ +Y+ R+ A + LG++P ++I S + EW++S A LY + E +N + +++V ++ VL K + ILE + VVVIE LS I++RDL++ + K + N I+YTSG+TG PKGVM++H ++V+ +T ++YLPL+HI ++ + C L D S +K +PT+ VP + ++ +GI + + K + + + N + G F L N+ L++ ++ LG + + GGAP+ T F +L + + AYGL+E + + +EY TC
Sbjct: 1942 GDYSYISYNGLRRQTSDLADGLMDLGIEPRSRIAILSESRQEWVVSMYAAFKNNYTLVTLYTNLGNDAVEEGLNETEVPLVIV-SDEVLPKFKGILEACPLIKYVVVIEAGNGKKSDLSVNYQNNFQFIMYRDLLK-----RRSKKKYNRSIPSTNDTAIIMYTSGSTGRPKGVMVNHGDAIAGLRSVISYTDFTTSDRYLAYLPLAHIMEIVAEMACLYCGVKIGYSSPLTLTDNGSKVKKGCKGDANVFKPTLMCAVPLLLQRIYKGIIDNMRKKGWAMEELFNYFVAYKIKWQXRG---FDXPLLNKTLFRTVRYFLG-GRVRLLLNGGAPISHETHLFTRTTLSVPVLQAYGLTELSGAAAIS-NEYDFSTC 2306 HSP 3 Score: 86.6557 bits (213), Expect = 4.094e-17 Identity = 118/486 (24.28%), Postives = 205/486 (42.18%), Query Frame = 0
Query: 70 GNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEED-----GFRPGEN----LILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYAL--KGSLVD-----TLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENG-DQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDF-FLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIG-----------GEICIKGRCVMMGYLFNEQATSEAFDKEG---YFHTG---------------RLKDLLLTSGGENVAPAPIENAIK 508
G + YD+ + A+ F L L + +++ EW ++ A LY + +N + + LVV + +L I ++K+ + ++V+E + +P EN I+++DL+ E DR + ++YTSG+TG PKGV++SH I KA++ + + + YLPL+H+ + V L ++ ++ L K S V +PT+ VP V E+ + + + + Q + ++ F M + G D +++K +F+K + +G R ISGG P+S T+ +L +PV + YGL+E + V SD T + P +K D + G GEI G + +GY N + T+E F +E +F TG R KDL+ SGGE ++ +E+ +K
Sbjct: 1415 GAYKFMTYDELKEESYNLAEGFRSLKLPTKTHIAMFAETRKEWILTAYAAFINNFTLVTLYTNLGEDGVIHGINETEVK-LVVCTVDMLPKIKKVIKSCPQIGVVIVMEANTGKTIDLKPYENNNVEFIMFRDLITRKSNE------DRYPAPTPRDPAIIMYTSGSTGTPKGVVISHKNIISGVKALMNVVTFKPSDR-YMGYLPLAHV-LELISEMVCLICGVKIGYSSPLTLTNKSSKVKRGCKGDANVFKPTLMCSVPLVLERIYKSVVDTMRRQGWAIEELFHYFVAYKMKWQXRGFDTPLLNKT----LFRKXRYFIG-GRVRLLISGGXPISHDTQSVSRTALCVPVLQGYGLTE--LTGGVTMSDPYDRTTCRVGPPLLGLKLKLVDWEEGNYTINDKSFPRGEIHCGGDSISIGYFKNPEKTAEDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILK 1884 HSP 4 Score: 58.5362 bits (140), Expect = 1.470e-8 Identity = 85/378 (22.49%), Postives = 155/378 (41.01%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLS---------ILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVIC-----CRGELYFADKSA-LKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK-FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDF-FLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTC 1047
G + TY + E A+ F L L +++++ EW+++ A LY + + +N + + LVV +L K++ ++++ + V+V+E + L I++RDLI E+ R I+YTSG+TG PKGV+ISH + K ++ + + YLPL+H+ ++ +IC L +KS+ +K +PT+ VP V E+ + + + + + Q + L Y + F L N+ L++K + +G + + GG P+ T +L + + YGL+E G + P Y + TC
Sbjct: 1415 GAYKFMTYDELKEESYNLAEGFRSLKLPTKTHIAMFAETRKEWILTAYAAFINNFTLVTLYTNLGEDGVIHGINETEVK-LVVCTVDMLPKIKKVIKSCPQIGVVIVMEANTGKTIDLKPYENNNVEFIMFRDLITRKSNED------RYPAPTPRDPAIIMYTSGSTGTPKGVVISHKNIISGVKALMNVVTFKPSDRYMGYLPLAHVLELISEMVCLICGVKIGYSSPLTLTNKSSKVKRGCKGDANVFKPTLMCSVPLVLERIYKSVVDTM----RRQGWAIEELFHYFVAYKMKWQXRGFDTPLLNKTLFRKXRYFIG-GRVRLLISGGXPISHDTQSVSRTALCVPVLQGYGLTELTGGVTMSDP--YDRTTC 1778
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000009272 (pep:novel supercontig:LSalAtl2s:LSalAtl2s591:175091:177076:1 gene:EMLSAG00000009272 transcript:EMLSAT00000009272 description:"augustus_masked-LSalAtl2s591-processed-gene-1.0") HSP 1 Score: 110.153 bits (274), Expect = 1.230e-24 Identity = 136/525 (25.90%), Postives = 226/525 (43.05%), Query Frame = 0
Query: 74 KW-NYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIE------------EDGFRPGE--NLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLI-YTSGTTGNPKGVMLSH-DIITFMTKAIVKKHDWTTA--KFIVVSYLPLSHIAASFLDIYVALY-----LKGEVYFADKYAL--KGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQ--MFKDASMHYYENGDQKVIHKIG------DKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLS-LDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAF-DKEG--YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVK 536
KW +Y+ I+ A LG P + IY+ +W IS LG +Y Y +N + ++ V+ + +L + ILK + I+ IE +DG N+ + +++ +G L+ S + + ++I YTSG+TGNPKG +LSH +I++ + + + ++YLPL+HI L++ + L G D + +G + D + ++PT VP + ++ +GI Q KV+Q FK+ ++ + + + K+G +KLIF+KV+++LG I GGAPLS DF + L + + + YGL+E CG D+ G G +P + K+V + GEI + G V MGY + T E+F +K+G +F TG R KDL+ GE V+ +E A K + P V G++ KF V + + K
Sbjct: 42 KWLSYNDVNEQIQSFAHGLRYLGFQPREKIVIYAETSAKWLISALGCFRNAYTLVTVYTNLGEDGVAYSLNQTDAKV-VITSEDLLPKLKGILKKGGHIHTIIYIEKPLEKMNIHAQNDDGSEQASEVNVYSFTEVIRIGVSSYSPDLDCYESWKATPEDIAIIMYTSGSTGNPKGALLSHANILSGILACCPYGXNLLVPPEEDRYIAYLPLAHIFEFTLEMTMILRGTSIGYSGPNTLTDAGXMIKRGHMGDA-RALKPTSMIAVPVILDRIFKGI----------QFKVSQEGAFKE-NLFTFCYQYRSMWRKLGFDTPIMNKLIFKKVRESLG-GEIQSIIGGGAPLSPEVHDFLETVLCVKISQGYGLTESCGGITLCHYDDYHVGEVGYPLPGAYIKLVNWEEGGYKVNNSTAPRGEIHVGGNVVAMGYYNMSEKTKESFYEKDGIRWFPTGDIGQLQPTGAFKIIDRKKDLVKLQMGEYVSLGKVEGAWKIH-PFVDAICSYGNSFKFDIVAVVVPDK 551 HSP 2 Score: 97.4413 bits (241), Expect = 9.256e-21 Identity = 101/401 (25.19%), Postives = 182/401 (45.39%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE------------EDGF-IADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSII-YTSGTTGNPKGVMISH-DMLT-------YTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVV-----ICCRGELYFADKSAL-KGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK---FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLS-LDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPST 1054
D+ +Y+ +I+ A LG +P + IY+ +W+IS LG +Y + Y +N + ++ V+ + +L K++ IL+ H+ ++ IE +DG A ++++ + ++I +G + L A + +II YTSG+TGNPKG ++SH ++L+ Y +V ++ ++YLPL+HI L++ ++ I G D + K + + ++PT VP + ++ +GI+ K+S + F N+ FCY + F + N++++KK+++SLG + Q GGAPL P DF + L + I YGL+E CG + +Y G G P
Sbjct: 39 ADYKWLSYNDVNEQIQSFAHGLRYLGFQPREKIVIYAETSAKWLISALGCFRNAYTLVTVYTNLGEDGVAYSLNQTDAKV-VITSEDLLPKLKGILKKGGHIHTIIYIEKPLEKMNIHAQNDDGSEQASEVNVYSFTEVIRIGVSSYSPDLDCYESWKATPEDIAIIMYTSGSTGNPKGALLSHANILSGILACCPYGXNLLVP----PEEDRYIAYLPLAHIFEFTLEMTMILRGTSIGYSGPNTLTDAGXMIKRGHMGDARALKPTSMIAVPVILDRIFKGIQFKVS---QEGAFKENLF---TFCYQYRSMWRKLGFDTPIMNKLIFKKVRESLG-GEIQSIIGGGAPLSPEVHDFLETVLCVKISQGYGLTESCGGITLCHYDDYHVGEVGYPLPGA 427
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000004934 (pep:novel supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9") HSP 1 Score: 108.227 bits (269), Expect = 6.204e-24 Identity = 131/527 (24.86%), Postives = 229/527 (43.45%), Query Frame = 0
Query: 68 RNGNWVKWNYDQY----YSDIKLTAKAFIK----LGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTL-KKIVVIEEDGFRPGE-------NLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSH--------DIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKG-EVYFADKYAL--KGSLVDT-----LKEVRPTMFFGVPRVWEK-FQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFF-LSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAFDKEG---YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTI 533
N K++ +Y Y D+ ++ F K L P + +Y+ + +W +S G F ++ TN Y +++ + +L+ + IL + L + I+ +E++ F + N+ + ++LE G K N+ + ++YTSG++G PKGV+LSH I++F+ + V+ K + YLPL+HI L + +AL+ KG + F+ L GS+V + E++PT VP + ++ ++ I++ S+N S++ ++ S + +I + L F+K++Q LG +SGGAPLS + +F + D+ + + YGL+E CG F +K DF G G P K+ + + GEI + G + MGY T E F +E +F TG R KD++ GE V+ +E + + P+V V G+ E+ V L I
Sbjct: 199 ENNTMTKYDQGEYEWLTYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGC-FKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQNLLKRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAVIMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNFVR-----LDKESYIGYLPLAHIFE--LSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKWRKLGMNTPII----NLLFFKKIQQFLG-GNVSTIVSGGAPLSAQAHEFMEIVFDLKLSQGYGLTETCGGFILGRKEDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYLGTH-PLVDIICVYGEPERNCIVGLII 711 HSP 2 Score: 79.337 bits (194), Expect = 4.420e-15 Identity = 93/402 (23.13%), Postives = 173/402 (43.03%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVI-------------EEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISH--------DMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRG-ELYFADKSAL--KGSLVRT-----LKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLG------NQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFF-LSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFP 1052
G++ TY R +K L P + +Y+ + +W++S G +Y + + + + ++++ N +L+++Q IL + L++ ++ E+D + IL + L + + E+ + +IA I+YTSG++G PKGV++SH ++++ + V K S + YLPL+HI L + + + +G + F+ + L GS+V+ + E++PT VP + ++ + I +K+S+ + + N FCY K + LG N + +KKI+ LG GGAPL +F + D+ + YGL+E CG F ++ G G FP
Sbjct: 209 GEYEWLTYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQN-LLKRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAV-----IMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNFVR----LDKESYIGYLPLAHIF--ELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFN------FCYEY---KSKWRKLGMNTPIINLLFFKKIQQFLG-GNVSTIVSGGAPLSAQAHEFMEIVFDLKLSQGYGLTETCGGFILGRKEDFSLGEVGHPFP 588
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000007052 (pep:novel supercontig:LSalAtl2s:LSalAtl2s3:1758018:1760492:-1 gene:EMLSAG00000007052 transcript:EMLSAT00000007052 description:"maker-LSalAtl2s3-snap-gene-17.37") HSP 1 Score: 97.8265 bits (242), Expect = 1.015e-20 Identity = 99/359 (27.58%), Postives = 166/359 (46.24%), Query Frame = 0
Query: 221 LIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTA---KFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVR-----------PTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFF-LSLDIPVQECYGLSECGIFCSVQK-SDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAFDKE---GYFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKS 537
++YTSG+TG PKGV+L+H + K++ + + K+I ++LPL+H+ ++ + ++G Y+ +L+DT ++ PT+ GVP V + +GI ++ ++ + + + D + NG + + +K++F K+ + +G + ISGGAPLS+ DF + L + V + YGL+E +V +D TGT G K+V D + GEI I G CV GY + T E F +E +F TG R KDL+ GE ++ +E+A+K PVV N + GD+ K V L + K+
Sbjct: 309 IMYTSGSTGVPKGVLLTHRNLVSTLKSLNSTFEIKISSNDKYI--AFLPLAHVLELLCEM--MMVIRG---VRIGYSSPNTLLDTSTMIKSGTHGDAAVLQPTVMAGVPLVLTRIYKGISQKMDSRGELFNSIFRFCYDYRLKSIRNG---LPTPLLNKILFGKIHKLIG-GKVKLIISGGAPLSDEXHDFXRVCLGVDVLQGYGLTETTASATVMDINDISTGTVGPPNQFLDIKLVNWEDGNYKVTEQPRPRGEILIGGDCVSNGYYKMPEKTKEDFLEERGKRWFRTGDIGEVTELGTIKIIDRKKDLVKLQYGEYISLGKVESALKT-CPVVDNICIYGDSFKSNCVALIVPNKT 655 HSP 2 Score: 63.929 bits (154), Expect = 2.834e-10 Identity = 62/208 (29.81%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 839 IIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKIS----IVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGS-LVRTLKE-----VRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN-----LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFF-LSLDIVIKVAYGLSE 1030
I+YTSG+TG PKGV+++H L T K++ + KIS +++LPL+H+ ++ +VI Y + + L S ++++ ++PTV GVP V + +GI + K+ + +I + FCY+ K I+N L N++L+ KI +G + + GGAPL DF + L + + YGL+E
Sbjct: 309 IMYTSGSTGVPKGVLLTHRNLVSTLKSL--NSTFEIKISSNDKYIAFLPLAHVLELLCEMMMVIRGVRIGYSSPNTLLDTSTMIKSGTHGDAAVLQPTVMAGVPLVLTRIYKGISQ---KMDSRGELFNSIFR---FCYDY--RLKSIRNGLPTPLLNKILFGKIHKLIG-GKVKLIISGGAPLSDEXHDFXRVCLGVDVLQGYGLTE 505
BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Match: EMLSAP00000001709 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17") HSP 1 Score: 87.0409 bits (214), Expect = 1.422e-17 Identity = 116/485 (23.92%), Postives = 198/485 (40.82%), Query Frame = 0
Query: 75 WNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVI--EEDGFRPGE-----NLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSH----DIITFMTKAIVKKHDWT---TAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSD-FKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVG 521
+ Y+ +S + A ++LG + + N PE+ I+ +G G + PT ++NS + ++ L L+ I + + ++KI+VI EE P + + ++ D ++E ID D L+ L Y+SGTTG PKGV L+H + IT ++ + H++ + I ++ LP HI A + L ++ K+ + +++L + +PT VP + S K S + +M + + +K ++K L FQ E YG++EC VQ D G+CG +IP+T K++ D D G GE+C+ G +M GY N++AT + + G+ HTG RLK+L+ G + VAP+ +E+ I+ Y P V + VG
Sbjct: 88 YTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGL-FLQNIKQSCEIYSGIQKIIVIGMEET---PSDCKSFMEMAIFDDGSFFDQRETIDPHKDILA---------LPYSSGTTGPPKGVALTHSNLVNNITQLSHPSI--HNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPE-MFINSLVQYKPTHMLVVPPLVS-----FMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQ-------------------------------------EGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKII---DIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKII-QNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQ-VAPSELEDLIRKY-PGVDDVAXVG 508
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|82209800|sp|Q7ZYC4.1|ACBG2_XENLA (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2) HSP 1 Score: 465.307 bits (1196), Expect = 1.345e-145 Identity = 255/622 (41.00%), Postives = 366/622 (58.84%), Query Frame = 0
Query: 26 TTKPDKGVHIRTESSEFLSF---SVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKA---IVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGD--QKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSD-FKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
T + D V +R E S+ S ++ + + G ALA + W K +Y QYY ++ AK FIKLGL+ ++ V I FN EWFI+++GAIFAGG + G+Y TNS+ Y+ N I+VVEN + L+ I + + LK I+ +++ NL WK+ ++LG+ +L+ +S NQCC+LIYTSGTTG PKGVMLSHD IT+ A V+ + T + IVVSYLPLSHIAA +DI++ + G YFA ALKGSL TL+EVRPT F GVPRVWEK QE +K + K+A K + NG + + + +KL+F+KV++ALGLDRC + +G AP+++ T +FFLSL+IPV E YG+SE ++ D F+ +CG +I K + D D GEI GR V MGYL E T E+ D+EG+ H TGR+K+L++T+GGEN+ P P E+A+K +P++SNA+++GD +KF+S+ LT+K N D GEP ELT + + +G T V +I+ K V AIQ+G++ N+++ SNA KV+KW IL ++ S+ GE+G T+
Sbjct: 86 TAQRDSAVKLRLEDSDVASLPPVTIHQLFQETVNKYGDYVALASKQGDQWHKMSYKQYYEQCRIAAKGFIKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQVQDQLPHLKAIIQYKDELKEKRPNLYTWKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAAAAGKTVRLREATDLQEIVVSYLPLSHIAAQMIDIWLTMKYGGATYFAQPDALKGSLAITLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKMATWAKGVGLETNLKKMNGSTPHPMKYHVANKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTK-IHQPDSDGSGEILFWGRHVFMGYLNMEDKTHESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPTEDAVKEQVPIISNAMLIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIQFCRQIGSKATLVSDIVGGKDTAVYAAIQEGVNSVNEKSTSNAQKVQKWLILEKDFSITGGELGPTM 706 HSP 2 Score: 379.022 bits (972), Expect = 9.128e-114 Identity = 235/661 (35.55%), Postives = 356/661 (53.86%), Query Frame = 0
Query: 627 SSSVLITEKIE-----DAKEYTTTLPDGSVIIRRKKDD---LQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTA----KTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIF---------PSTQGRKKELL------------------------------ITAGGEN--------IAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
SS V++ +E D K +T D +V +R + D L P+++ + K G +A+A K+ W + +Y QYY + R+AAK FIKLGL+ +H V I FN EW I+++GAIFAGGFA G+Y +NSA Y+ N I+VVEN LQK+ + + + H LK ++ +D + ++ W++ ++LGK + QL Q + + NQCC++IYTSGTTG PKGVM+SHD +T+TA KTV + + +VSYLPLSHIAA +DI++ + G YFA ALKGSL TL+EVRPT F GVPRV+EK QE I+ K++ K TN+ K N + ++ N++++KK++ +LGLD+C + G AP+ T +FFLSL+I + YG+SE G + P ++ +CG + P + G + L I EN I ++I +N PV +SN + IGD KKF+S+ LT K VN+DT G+P EL+ ++C +G +A+ V++I+ + V +AIQ+G++ NE SNA +V+KW ++ ++ S+ GE+GPT+KLKR +K++
Sbjct: 65 SSDVMVHSAVENLPTSDMKLWTAQR-DSAVKLRLEDSDVASLPPVTIHQLFQETVNKYGDYVALASKQGDQWHKMSYKQYYEQCRIAAKGFIKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQVQDQLPH-LKAIIQYKDELKEKRPNLYTWKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAAAAGKTVRLREATDLQEIVVSYLPLSHIAAQMIDIWLTMKYGGATYFAQPDALKGSLAITLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKMATWAKGVGLETNLKK-----MNGSTPHPMKYHVANKLVFKKVRKALGLDRCTKCYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDAFRITSCGKVISGCKTKIHQPDSDGSGEILFWGRHVFMGYLNMEDKTHESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPTEDAVKEQVPIISNAMLIGDKKKFLSMLLTLKCNVNADT-GEPEDELTPEAIQFCRQIGSKATLVSDIVGGKDTAVYAAIQEGVNSVNEKSTSNAQKVQKWLILEKDFSITGGELGPTMKLKRPVVAKMY 717 HSP 3 Score: 114.39 bits (285), Expect = 1.780e-24 Identity = 55/134 (41.04%), Postives = 89/134 (66.42%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ ED +K +P++SN + IGD KKF+S+ LT K N + + G+P EL+ +C + +A+ V++I+ K V +AIQ+G++ N+ + SNA KV+KW ++ ++ S+ GE+GPT+
Sbjct: 573 KELIITAGGENIPPVPTEDAVKEQVPIISNAMLIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIQFCRQIGSKATLVSDIVGGKDTAVYAAIQEGVNSVNEKSTSNAQKVQKWLILEKDFSITGGELGPTM 706
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|166198360|sp|Q5ZKR7.2|ACBG2_CHICK (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2) HSP 1 Score: 464.151 bits (1193), Expect = 8.121e-145 Identity = 276/682 (40.47%), Postives = 384/682 (56.30%), Query Frame = 0
Query: 10 TDKMFPNKQALASSYT------TTKPDKGVHIRTESSEFLSFSVPNMVKNHCLAGGSKT----ALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVEN-LQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK---HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQKVI--HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSV--LITEKIEDAKEYTTTLPDGSVII 653
T++ NK LASS TT+ D V +R + E + P V SK ALA +NG WVK Y YY AK+F+KL L+ F+ VCI FN PEWFI+++GAIFAGGL+ G+Y TNS Y+ N ILVVEN Q R I + +K I+ E+ N W++ L+LG +L + + NQCC+LIYTSGTTG PKGVMLSHD +T+ + A + + T + +VVSYLPLSH+AA +DI++ + G+V+FA ALKG+LVDTL+EVRPT F GVPRVWEK +E +K + KVA K + + NG +V ++ +L+++KV++A+GLDRC + +G AP+S T +FFLSL+IPV E YG+SE G F+ +CG + + K D I GEIC GR + MGYL E+ T EA DK+G+ H TGR+K+L++T+GGENV P PIE+A+K P++SNA++VGD KF+++ LT+K + + E GEP +LT + + LG T V EI+ K V AIQ + E NKRA+SNA K++KW +L ++ S+ GE+G T+ ++ LI E DA TTT S ++
Sbjct: 57 TERSQMNKTGLASSSRPASNVWTTQQDGEVKLRMDE-EGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQACRKSLEIEHKLPHMKAIIQYGEELKEKRPNQYSWREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGI-GEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFYADANTPTTTEERTSAVM 736 HSP 2 Score: 380.948 bits (977), Expect = 3.850e-114 Identity = 230/651 (35.33%), Postives = 349/651 (53.61%), Query Frame = 0
Query: 620 VNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNX-VLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTA----KTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYG-----------------LSECG---------------------------IFSDFAPSEYKK---------------GTCGSI-FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
+N G SSS A TT DG V +R ++ + P +V + K G A+A K+NG W++ TY YY + AAK+F+KL L+ FH V I FN PEW I+++GAIFAGG A G+Y +NS Y+ N ILVVEN+ +K I + H+ ++ E+ WR+ ++LG+ + QL++ + + NQCC++IYTSGTTG PKGVM+SHD LT+T+ ++++ K+ +VSYLPLSH+AA +DI++ + G+++FA ALKG+LV TL+EVRPT F GVPRV+EK +E ++ +K ++ + + K N G+ + N L Q++YKK++ ++GLD+C + G AP+ T +FFLSL+I + YG L+ CG IF + E K G C F GR KEL+ITAGGEN+ P+ IED +K P++SN + +GD KF+++ LT K +N+++ G+P +L+ E+C LG +A+ V+ II + V +AIQ + N+ VSNA +++KW ++ ++ S+ GE+GPT+KLKR
Sbjct: 62 MNKTGLASSS-------RPASNVWTTQQDGEVKLRMDEEGMGSEAPKTVHEVFQEAVSKYGDYYALASKKNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHTQACRKSLEIEHKLPHMKAIIQYGEELKEKRPNQYSWREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTKCFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSIPQAFRLTSCGKEMAGCRTLIHKPDADGIGEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTES-GEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKR 704 HSP 3 Score: 112.079 bits (279), Expect = 9.595e-24 Identity = 53/134 (39.55%), Postives = 88/134 (65.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFK-VNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IED +K P++SN + +GD KF+++ LT K + + ++G+P +L+ ++C L +A+ V+ II K V +AIQ + + NK AVSNA K++KW ++ ++ S+ GE+GPT+
Sbjct: 567 KELIITAGGENVPPVPIEDAVKEACPIISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYAAIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTM 700
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|296434386|sp|Q5FVE4.2|ACBG2_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2; AltName: Full=Bubblegum-related protein; AltName: Full=PRTD-NY3) HSP 1 Score: 444.121 bits (1141), Expect = 1.689e-138 Identity = 245/594 (41.25%), Postives = 352/594 (59.26%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKN----QKKSQMKVAQM-FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIG 616
++P + G+ ALA W N++QYY + AK+ IKLGL+ F+ V I FN EWFI+ +GAI AGGL G+Y TNS+ +Y++ +++ IL+VEN Q L+ I I ++ K ++ + NL W D +ELGR +L + NQC LIYTSGTTG PKGVMLSHD IT++ A+ K T VVSYLPLSHIAA +DI+V + + YFA ALKG+LV TLKEV+PT+F GVP++WEK E +K+ S +KK+ + + FK S + V +++ L+F KVK +LGLD C FISG APL++ T +FFLSLDIP+ E YGLSE G +++++ +CG I+ + + + D I GEIC+ GR + MGYL +E T+EA D EG+ H TG +K++L+T+GGENV P P+E +K +P++SNA++VGD KF+S+ LT+K + N+ GEP +L + LG +TV EI+ + P V KAIQ+GI+ N+ A++NA +++KW IL ++ S+ GE+G
Sbjct: 51 MTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNMLFQQNKDGI-GEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELG 643 HSP 2 Score: 360.147 bits (923), Expect = 1.054e-107 Identity = 232/636 (36.48%), Postives = 338/636 (53.14%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKI-SIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKL----KKSQKFITNI-----LKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIF----------------------------------PSTQGRKKELLITAG--GE-----------NIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFKVN-SDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
K E TT DG V++R K PM++P F + + + G+ A+A K W ++QYY R AAK+ IKLGL+ FH V I FN EW I+ +GAI AGG G+Y +NSA + +Y++ +++ IL+VEN+ LQK+ I ++ LK ++ + W D +ELG++ + QL+Q + + ANQC +IYTSGTTG PKGVM+SHD +T+ A V + T K ++VSYLPLSHIAA +DI+V I YFA ALKG+LV TLKEV+PTVF GVP+++EK E +++ +K KK+ + NI K YNT + + K L ++ K+K SLGLD C F G APL T +FFLSLDI I YGLSE G + + Y+ +CG I +T+ E + +G G+ +I ILI +N P+ +SN + +GD KF+S+ LT K + +G+P +L+ +C LG +ASTVT I++ Q P V AIQ+GI+ N+ ++NA R++KW ++ ++ S+ GE+GP +KLKR+
Sbjct: 20 KTEVTPRLWTTCRDGEVLLRLSKHGPGHETPMTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTL----VFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNMLFQQNKDGIGEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRH 651 HSP 3 Score: 104.375 bits (259), Expect = 2.008e-21 Identity = 50/134 (37.31%), Postives = 89/134 (66.42%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+ PI +E +K +P++SN + +GD KF+S+ LT K ++ +G+P +L+ ++C L +ASTVT I++ + V AIQ+GI+ N+ A++NA +++KW ++ ++ S+ GE+GP +
Sbjct: 513 KEILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMM 646
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|166198274|sp|A1L1K7.1|ACBG2_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2) HSP 1 Score: 419.083 bits (1076), Expect = 2.600e-129 Identity = 234/600 (39.00%), Postives = 351/600 (58.50%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKN-VTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDW---TTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKE---QSKNQKKSQMKVAQM--FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
++P + + G+ ALA W + QYY + A++ IKLGL F+ V I FN EW ++ LGAI AGGL G+Y TNS+ +Y++ + +L+VEN Q L+ I I + + T+K IV NL W+D +ELG +L+ + NQC +IYTSGTTG+PKGVMLSHD IT+ A+ ++ + + + +VSYLPLSHIAA +DI++ + + +FA AL+G+LV TL+EV+PT F GVPRVWEK Q+ IKE +S N +K A+M K + D + +++ L+F KV+ +LGLD C F SG +PLS+ +FFLSLDIP+ E YG++EC G KS ++ +CG ++ + K + D + GE+C+ GR V MGYL E AT E D++G+ H TGR+K++L+T+GGEN+ P PIE +K +P++S+A++VGD KF+S+ LT+K ++++ GEP +L + LG TV +IL + P V AIQ GID N++A+S++ +++KW IL ++ S++ GE+G T
Sbjct: 47 MTIPELFQESVERFGAYPALASKNGKKWDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALGAILAGGLCVGIYATNSAEACQYVIKQANVNVLIVENDQQLQKILSIPPDKMETVKAIVQYRLPLMENSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTGSPKGVMLSHDNITWTAGAMAREIELIHVSGKQDTIVSYLPLSHIAAQLMDIWIPIKVGVLTFFAQPDALRGTLVYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAFAWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNCHTFFSGASPLSQDVSEFFLSLDIPIGEIYGMTECSGPHTVSCKSIYRVLSCGKVLNGCKNMLYKQNKDGV-GEVCMWGRHVFMGYLGKEDATLEVLDEDGWLHSGDIGRLDSHDFLYITGRIKEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPT 645 HSP 2 Score: 375.555 bits (963), Expect = 2.657e-113 Identity = 229/636 (36.01%), Postives = 342/636 (53.77%), Query Frame = 0
Query: 639 AKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEK----HGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVI-----------------KVAYGLSECG---------------------------IFSDFAPSE------------YKKGTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
A +T DG V++R K PM++P + + + G+ A+A K W T+SQYY R AA++ IKLGL+ FH V I FN EW+++ LGAI AGG G+Y +NSA +Y++ + +L+VEN+ LQK+ I + +K +V + + ++ W+D +ELG QL + + + ANQC IIYTSGTTG+PKGVM+SHD +T+TA + + H K+ +IVSYLPLSHIAA +DI++ I +FA AL+G+LV TL+EV+PT F GVPRV+EK Q+ I+E ++K +K K NT G + N + +++ K++ SLGLD C F G +PL ++FFLSLDI I K Y + CG +F + E G G + F GR KE+LITAGGENI PI IE +K +P++S+ + +GD KF+S+ LT K +D +G+P +L+ +C LG +A TV++I++ + P V +AIQ GID+ N+ VS++ R++KW ++ ++ S+ GE+GPT KLKR+ ++
Sbjct: 20 APRLWSTHCDGEVLLRLSKHGPGHETPMTIPELFQESVERFGAYPALASKNGKKWDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALGAILAGGLCVGIYATNSAEACQYVIKQANVNVLIVENDQQLQKILSIPPDKMETVKAIVQYRLPLMENSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTGSPKGVMLSHDNITWTAGAMAREIELIHVSGKQDTIVSYLPLSHIAAQLMDIWIPIKVGVLTFFAQPDALRGTLVYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAFAWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNCHTFFSGASPLSQDVSEFFLSLDIPIGEIYGMTECSGPHTVSCKSIYRVLSCGKVLNGCKNMLYKQNKDGVGEVCMWGRHVFMGYLGKEDATLEVLDEDGWLHSGDIGRLDSHDFLYITGRIKEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPTSKLKRDLITQ 655 HSP 3 Score: 104.76 bits (260), Expect = 1.540e-21 Identity = 52/133 (39.10%), Postives = 89/133 (66.92%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG ENI PI IE +K +P++S+ + +GD KF+S+ LT K +D+ +G+P +L+ +C L +A TV++I++ + V +AIQ GID N+ AVS++ +++KW ++ ++ S+ GE+GPT
Sbjct: 513 KEVLITAGGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPT 645
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|74947350|sp|Q9V3S9.1|BGM_DROME (RecName: Full=Very long-chain-fatty-acid--CoA ligase bubblegum) HSP 1 Score: 418.698 bits (1075), Expect = 4.392e-129 Identity = 244/645 (37.83%), Postives = 364/645 (56.43%), Query Frame = 0
Query: 7 NSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGN-WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDG---FRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTT-AKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQK----VIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE---CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP-PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+++ ++ PN+ A +Y TT V IR + SVP ++K G AL N + Y QY + AKAFIKLGL+ ++V + +FNC EWF S +GAI A G+ AG+Y TNS+ ++++ +S QI+VV++ + + I I + LK + I+E + + W ++ + + D+ RL ++++N+CC L+YTSGT G PKGVMLSHD ITF + IVK D +VSYLPLSH+AA +DIY ++ G ++FADK ALKG+LV +L++ RPT F GVPRV+EKFQE + + + + +A K ++ +Y K +KI LI KVKQALG DR L S AP+S T+ +FLSLD+ + + +G+SE C C T G +P +K + + D + GE+CI+GR V MGY+ N++ T E+ D + + H TGR K++++T+GGEN+ P IEN IK L +SNA +VG+ K+++V +T+K + ++D GEP EL+ K+LG + TV +IL P P V K+I+ I ANK++ISNA KV+K+ ILP + S+ GE+G TL
Sbjct: 5 DALYNRPGPNRLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTIC--LPDSVGLNTIGKTLPGCESKFI-NKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646 HSP 2 Score: 277.715 bits (709), Expect = 1.338e-78 Identity = 160/444 (36.04%), Postives = 239/444 (53.83%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIK--RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE---------EDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW--TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK----FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGR 1057
++ A Y TT +V IR KD + +P+SVP +K G A+ K +NG + TY QY +++ AKAFIKLGL+ H+V + +FNC EW S +GAI A G G+Y +NSA ++++ +S QI+VV++ + K+ I + + L + I+ EDG+ W ++ + ++ EDQ RL N+A N+CC ++YTSGT G PKGVM+SHD +T+ + +V+ SIVSYLPLSH+AA +DIY G ++FADK ALKG+LV++L++ RPT F GVPRVYEKFQE + S +K + + K + K F + ++ K+K +LG D+ + AP+ P T +FLSLD+ I A+G+SE G + P T G P + +
Sbjct: 15 RLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNG-YHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYR------WSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESK 451 HSP 3 Score: 132.109 bits (331), Expect = 3.208e-30 Identity = 66/156 (42.31%), Postives = 103/156 (66.03%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFN 1204
+ S GR KE++ITAGGENI P+ IE+ IK L +SN +G+ +K+++V +T K D + G+P ELS+ W SLG E TV++I+ P P+V +I+ I AN+ +SNA +V+K++++P + S+ GE+GPTLK+KRN SK++ +
Sbjct: 504 YVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYAD 659 HSP 4 Score: 108.612 bits (270), Expect = 1.014e-22 Identity = 56/137 (40.88%), Postives = 89/137 (64.96%), Query Frame = 0
Query: 1312 KEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
+ K++ ITAG ENI P+ IE+ IK L +SN +G+ +K+++V +T K DK+ G+P ELS+ W SL E TV++I+ P +V +I+ I +ANK ++SNA KV+K++++P + S+ GE+GPTL
Sbjct: 510 RSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|81902685|sp|Q924N5.1|ACBG1_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; AltName: Full=Gonadotropin-regulated long chain acyl CoA synthetase; Short=GR-LACS) HSP 1 Score: 418.313 bits (1074), Expect = 2.844e-128 Identity = 235/619 (37.96%), Postives = 350/619 (56.54%), Query Frame = 0
Query: 26 TTKPDKGVHIRTES-SEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKN----QKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
TT+ D V +R E L ++V M G+ +AL R W + +Y QYY + AK F+KLGL+ ++V I FN PEWF S +G +FAGG+ G+Y T+S +Y+ ++ R ++VV+ + L I I K++ LK +V+ +E + N+ ++L+ELG++ + L+ + NQCC L+YTSGTTGNPKGVMLS D IT+ + + D A+ +VVSYLPLSHIAA D++ + +V FAD ALKG+LV+TL+EV PT GVPRVWEK E I+E + ++K + + + ++ N + ++ D L+ +V+QALG +C + G AP++ T+ FFL L+I + YGLSE G +++ + G ++P K+V D I GEIC+ GR + MGYL E T EA D EG+ H TGRLK+L++T+GGENV P PIE A+K LP++S+A+++GD KF+S+ LT+K N E EPT LT+ + + +G +TV EI+ K V +AI +GI N A + ++KW IL R+ S+ GE+G T+
Sbjct: 80 TTRADGRVRLRLEPFCTQLPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGI-GEICLWGRTIFMGYLNMEDKTHEAIDSEGWLHTGDMGRLDDDGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLNPETSEPTDNLTEQAVEFCQRVGSKASTVSEIVGQKDEAVYQAIHEGIQRVNANAAARPYHIQKWAILERDFSISGGELGPTM 697 HSP 2 Score: 326.25 bits (835), Expect = 5.532e-95 Identity = 211/625 (33.76%), Postives = 326/625 (52.16%), Query Frame = 0
Query: 644 TTLPDGSVIIRRKKDDLQ-PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILW--RDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISK----LKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQC--------------QEFSCG-------GAPLMPTTTDFFLS------------------LDIVIKVAYGLSEC-----GIFSDFAPSEYKK------------GTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
TT DG V +R + Q P +V K G+ A+ KR W R +Y QYY R AK F+KLGL+ H+V+I FN PEW S +G +FAGG TG+Y ++S +Y+ ++ R ++VV+ L+K+ I +++ HL VV+ +E K++ ++ +LIELG+ E+ L + NQCC ++YTSGTTGNPKGVM+S D +T+TA+ + + ++ +VSYLPLSHIAA D++ I ++ FAD ALKG+LV TL+EV PT GVPRV+EK E I+E ++ +K + ++ + + K F L + ++ +++ +LG +C Q F G G L +T F+S + +V + A G+ E IF + E K G G + F GR KEL+ITAGGEN+ P+ IE+ +K LP++S+ + IGD +KF+S+ LT K +N +T+ +P+ L+ E+C +G +ASTV+ I+ + V AI +GI N + ++KW+++ R+ S+ GE+GPT+KLKR
Sbjct: 80 TTRADGRVRLRLEPFCTQLPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPP--PKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTHEAIDSEGWLHTGDMGRLDDDGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLNPETS-EPTDNLTEQAVEFCQRVGSKASTVSEIVGQKDEAVYQAIHEGIQRVNANAAARPYHIQKWAILERDFSISGGELGPTMKLKR 701 HSP 3 Score: 104.76 bits (260), Expect = 1.963e-21 Identity = 49/134 (36.57%), Postives = 84/134 (62.69%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVN-SDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IE+ +K LP++S+ + IGD +KF+S+ LT K + + +P+ L+ ++C + +ASTV+ I+ K V AI +GI + N A + ++KW+++ R+ S+ GE+GPT+
Sbjct: 564 KELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLNPETSEPTDNLTEQAVEFCQRVGSKASTVSEIVGQKDEAVYQAIHEGIQRVNANAAARPYHIQKWAILERDFSISGGELGPTM 697
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|81903322|sp|Q99PU5.1|ACBG1_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; Short=mBG1; AltName: Full=Gonadotropin-regulated long chain acyl CoA synthetase; Short=GR-LACS; AltName: Full=Lipidosin) HSP 1 Score: 413.305 bits (1061), Expect = 2.119e-126 Identity = 232/619 (37.48%), Postives = 349/619 (56.38%), Query Frame = 0
Query: 26 TTKPDKGVHIRTES-SEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKN----QKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
TT+ D V +R E ++V M G+ +AL R W + +Y QYY + AK F+KLGL+ ++V I FN PEWF S +G +FAGG+ G+Y T+S +Y+ ++ R ++VV+ + L I I K++ LK +V+ +E + N+ ++L+ELG++ + L+ + NQCC L+YTSGTTGNPKGVMLS D IT+ + + D A+ +VVSYLPLSHIAA D++ + +V FAD ALKG+LV+TL+EV PT GVPRVWEK E I+E + ++K + + + ++ N + ++ D L+ +V+QALG +C + G AP++ T+ FFL L+I + YGLSE G +++ + G ++P K+V D I GEIC+ GR + MGYL E T EA D EG+ H TGRLK+L++T+GGENV P PIE A+K LP++S+A+++GD KF+S+ LT+K + E EPT LT+ + + +G +TV EI+ + V +AI +GI N A + ++KW IL R+ S+ GE+G T+
Sbjct: 80 TTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPPPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGI-GEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTM 697 HSP 2 Score: 332.028 bits (850), Expect = 5.087e-97 Identity = 211/626 (33.71%), Postives = 324/626 (51.76%), Query Frame = 0
Query: 644 TTLPDGSVIIRRKKDDLQ-PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILW--RDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISK----LKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAP---------------------LMPTTTDFFLS------------------LDIVIKVAYGLSEC-----GIFSDFAPSEYKKGTCGSI------------------FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDT-NGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
TT DG V +R + Q P +V K G+ A+ KR W R +Y QYY R AK F+KLGL+ H+V+I FN PEW S +G +FAGG TG+Y ++S +Y+ ++ R ++VV+ L+K+ I +++ HL VV+ +E K++ ++ +LIELG+ E+ L + NQCC ++YTSGTTGNPKGVM+S D +T+TA+ + + ++ +VSYLPLSHIAA D++ I ++ FAD ALKG+LV TL+EV PT GVPRV+EK E I+E ++ +K + ++ + + K F L + ++ +++ +LG +CQ+ G AP L +T F+S + +V + A G+ E IF + E K TC +I F GR KEL+ITAGGEN+ P+ IE+ +K LP++S+ + IGD +KF+S+ LT K D +P+ L+ E+C +G +ASTV+ I+ + V AI +GI N + ++KW+++ R+ S+ GE+GPT+KLKR
Sbjct: 80 TTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKDKWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILKIWKDLPHLKAVVIYQEPP--PKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRLADYLVLARVRQALGFAKCQKNFYGAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHFMSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDK--TCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKR 701 HSP 3 Score: 105.916 bits (263), Expect = 7.671e-22 Identity = 49/134 (36.57%), Postives = 84/134 (62.69%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IE+ +K LP++S+ + IGD +KF+S+ LT K D + +P+ L+ ++C + +ASTV+ I+ + V AI +GI + N A + ++KW+++ R+ S+ GE+GPT+
Sbjct: 564 KELIITAGGENVPPVPIEEAVKMELPIISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQRVGSKASTVSEIVGQRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTM 697
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|123779881|sp|Q2XU92.1|ACBG2_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2; AltName: Full=Bubblegum-related protein) HSP 1 Score: 411.379 bits (1056), Expect = 2.189e-126 Identity = 230/600 (38.33%), Postives = 348/600 (58.00%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKN-VTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDW---TTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKE---QSKNQKKSQMKVAQM--FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
++P + + + ALA W + QYY + AK+ IKLGL F V I FN EW ++ LG I AGGL G+Y TNS+ +Y++ + IL+VEN Q L+ I I + + T+K IV + NL W D +ELG +L+ + NQC ++YTSGTTG PKGV+LSHD IT+ A+ ++ + + + +VSYLPLSHIAA DI++ + + +FA AL+G+LV TL+EV+PT+F GVPR+WEK Q+ IKE +S +K A+M K + D + +++ L+F KV+ +LGLD C F S +PLS+ +FFLSLDIP+ E YG+SEC G KS ++ +CG ++ + + + + GE+C+ GR V MGYL E+AT EA D+ G+ H TGR+K++L+T+GGENV+P PIE +K +P++S+A++VGD KF+ + LT+K +++ + GEP ++L+ K + LG TTV +IL + V AIQ GID N++A+S++ +++KW IL ++ S++ GE+G T
Sbjct: 47 MTIPELFQESAERFSAYPALASKNGKKWDTLTFSQYYEMCRKAAKSLIKLGLQRFQCVGILGFNSVEWVVTALGTILAGGLCVGIYATNSAEACQYVIQQANVSILIVENDQQLQKILLIPPDKMETVKAIVQYKLPLMESMANLYSWNDFMELGNDIPNIQLDRVILSQKANQCAVILYTSGTTGTPKGVLLSHDNITWTAGAMSQEMEINRVSGKQNTIVSYLPLSHIAAQLTDIWIPIKIGALTFFAQPDALRGTLVYTLQEVKPTLFMGVPRIWEKMQDTIKENVARSSRLRKKAFAWAKMLGLKVNTKRMLGKRDIPMNYRMAKALVFAKVRTSLGLDNCHAFFSSASPLSQDVSEFFLSLDIPIGEIYGMSECSGPHTVSNKSVYRVLSCGKVLSGCKNMLYNQNKEGV-GEVCMWGRHVFMGYLNKEEATLEALDENGWLHSGDIGRLDSHDFLYITGRIKEILITAGGENVSPIPIETLVKEKIPIISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQMLGSQATTVSDILKSRDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPT 645 HSP 2 Score: 355.14 bits (910), Expect = 7.429e-106 Identity = 218/648 (33.64%), Postives = 342/648 (52.78%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLP-------DGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL-WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWT----KKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQ-----------------GR--------KKELLITAGGEN----------------------------------IAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
E +E + T+ +P DG V++R K PM++P + + + + A+A K W T+SQYY R AAK+ IKLGL+ F V I FN EW+++ LG I AGG G+Y +NSA +Y++ + IL+VEN+ LQK+ I + +K +V + + ++ W D +ELG QL + + + ANQC I+YTSGTTG PKGV++SHD +T+TA + ++ K+ +IVSYLPLSHIAA DI++ I +FA AL+G+LV TL+EV+PT+F GVPR++EK Q+ I+E +++ + +K K NT G + N + +++ K++ SLGLD C F +PL ++FFLSLDI I YG+SEC G + S Y+ +CG + + GR K+E + A EN ++PI IE +K +P++S+ + +GD KF+ + LT K +D +G+P +LS K +C LG +A+TV++I++ + V +AIQ GID+ N+ +S++ R++KW ++ ++ S+ GE+GPT KLKR+ ++
Sbjct: 8 EDLERGTDATSAVPRLWSTHCDGEVLLRLSKHGPGHETPMTIPELFQESAERFSAYPALASKNGKKWDTLTFSQYYEMCRKAAKSLIKLGLQRFQCVGILGFNSVEWVVTALGTILAGGLCVGIYATNSAEACQYVIQQANVSILIVENDQQLQKILLIPPDKMETVKAIVQYKLPLMESMANLYSWNDFMELGNDIPNIQLDRVILSQKANQCAVILYTSGTTGTPKGVLLSHDNITWTAGAMSQEMEINRVSGKQNTIVSYLPLSHIAAQLTDIWIPIKIGALTFFAQPDALRGTLVYTLQEVKPTLFMGVPRIWEKMQDTIKENVARSSRLRKKAFAWAKMLGLKVNTKRMLGKRDIPMNYRMAKALVFAKVRTSLGLDNCHAFFSSASPLSQDVSEFFLSLDIPIGEIYGMSECSGPHTVSNKSVYRVLSCGKVLSGCKNMLYNQNKEGVGEVCMWGRHVFMGYLNKEEATLEALDENGWLHSGDIGRLDSHDFLYITGRIKEILITAGGENVSPIPIETLVKEKIPIISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQMLGSQATTVSDILKSRDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPTSKLKRSVITQ 655 HSP 3 Score: 111.309 bits (277), Expect = 1.329e-23 Identity = 52/133 (39.10%), Postives = 91/133 (68.42%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG EN++PI IE +K +P++S+ + +GD KF+ + LT K +D K+G+P +LS K +C L +A+TV++I++ + V +AIQ GID N+ A+S++ +++KW ++ ++ S+ GE+GPT
Sbjct: 513 KEILITAGGENVSPIPIETLVKEKIPIISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQMLGSQATTVSDILKSRDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPT 645
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|122135920|sp|Q2KHW5.1|ACBG1_BOVIN (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1) HSP 1 Score: 403.675 bits (1036), Expect = 7.772e-123 Identity = 233/633 (36.81%), Postives = 352/633 (55.61%), Query Frame = 0
Query: 12 KMFPNKQALASSYTTTKPDKGVHIRTES-SEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQ----KKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
++ P ++AL TT+ D V +R + +V M G +A+ R G W +Y QYY + AK F+KLGL+ ++V I +FN PEWF S +GA+F GG+ G+Y T+S +Y+ + R I+VV+ + L I I K++ LK +V+ E ++ ++L+ELG + + L+ ++ NQCC+L+YTSGTTGNPKGVMLS D IT+ + + D A+ +VVSYLPLSHIAA D++ + +V FA+ ALKGSLV+TL+EV PT GVPRVWEK E I+E + +K + + + ++ + + ++ D L+ KV+QALG +C + G AP++ T+ FFL L+I + YGLSE G +++ + G ++P K+V + I GEIC+ GR + MGYL E T EA D EG+ H TGRLK+L++T+GGENV P PIE A+K LP++ NA+++GD KF+S+ LT+K + D EPT LT+ + + +G TTV E++ K V +AI++GI N A + ++KW IL ++ S+ GE+G T+
Sbjct: 56 QLNPPEEAL----WTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGI-GEICLWGRTIFMGYLNMEDKTCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTM 683 HSP 2 Score: 334.339 bits (856), Expect = 9.413e-98 Identity = 214/640 (33.44%), Postives = 329/640 (51.41%), Query Frame = 0
Query: 634 EKIEDAK------EYTTTLPDGSVIIRRKKDDLQ-PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISK----LKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAP---------------------LMPTTTDFFLS------------------LDIVIKVAYGLSEC-----GIFSDFAPSEYKKGTCGSI------------------FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
EK++DA+ TT DG V +R Q P +V K G AM KR G W +Y+QYY R AAK F+KLGL+ H+V+I +FN PEW S +GA+F GG TG+Y ++S +Y+ + R I+VV+ L+K+ I +++ HL VV+ E + +L+ELG E+ L ++ NQCC+++YTSGTTGNPKGVM+S D +T+TA+ + + ++ +VSYLPLSHIAA D++ I ++ FA+ ALKGSLV TL+EV PT GVPRV+EK E I+E ++ +K + ++ + ++ K F L + ++ K++ +LG +CQ+ G AP L T+ F+S + +V + A G+ E IF + E K TC +I F GR KEL+ITAGGEN+ P+ IE+ +K LP++ N + IGD +KF+S+ LT K D + +P+ L+ E+C +G +A+TV+ ++ + V AI++GI N + ++KW+++ ++ S+ GE+GPT+KLKR
Sbjct: 50 EKVKDAQLNPPEEALWTTRADGRVRLRIDPICSQTPRTVHQMFSTTLDKYGDLSAMGFKRQGTWEHISYTQYYLLARKAAKGFLKLGLERAHSVAILAFNSPEWFFSAVGAVFGGGIITGIYTTSSPEACQYIAYDCRANIIVVDTQKQLEKILKIWKHLPHLKAVVIYREAPPMRMPSVYTMEELMELGNEVPEEALDVIINAQKPNQCCALVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEIQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMEQIQEVAAQSGFIWRKMLLWAMSVTLEQNLTCPSSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNEDAEGIGEICLWGRTIFMGYLNMEDK--TCEAIDAEGWLHTGDTGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTMKLKR 687 HSP 3 Score: 103.219 bits (256), Expect = 5.036e-21 Identity = 47/134 (35.07%), Postives = 84/134 (62.69%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IE+ +K LP++ N + IGD +KF+S+ LT K D + +P+ L+ ++C + +A+TV+ ++ K V AI++GI + N A + ++KW+++ ++ S+ GE+GPT+
Sbjct: 550 KELIITAGGENVPPVPIEEAVKTELPIIRNAMLIGDQRKFLSMLLTLKCTLDPDTFEPTDNLTEQAVEFCQRVGSKATTVSEVVGKKDEAVYQAIEEGIQRVNMNAAARPYHIQKWAILEKDFSISGGELGPTM 683
BLAST of EMLSAG00000003336 vs. SwissProt
Match: gi|296434385|sp|Q96GR2.2|ACBG1_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; Short=hBG1; Short=hsBG; Short=hsBGM; AltName: Full=Lipidosin) HSP 1 Score: 398.667 bits (1023), Expect = 5.461e-121 Identity = 230/619 (37.16%), Postives = 341/619 (55.09%), Query Frame = 0
Query: 26 TTKPDKGVHIRTESS-EFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKN----QKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
TT+ D V +R + S L ++V M G AL R W +Y QYY + AK F+KLGL ++V I FN PEWF S +G +FAGG+ G+Y T+S +Y+ + +++V+ + L I I K + LK +V+ +E N+ ++ +ELG + + L+ + NQCC L+YTSGTTGNPKGVMLS D IT+ + + D A+ +VVSYLPLSHIAA D++ + +V FA+ ALKGSLV+TL+EV PT GVPRVWEK E I+E + ++K + + + ++ + + ++ D L+ KV+QALG +C + G AP+ T+ FFL L+I + YGLSE G +++ + G ++P K+V + I GEIC+ GR + MGYL E T EA D+EG+ H TGRLK+L++T+GGENV P PIE A+K LP++SNA+++GD KF+S+ LT+K + D + T LT+ + + +G TTV EI+ K V +AI++GI N A + ++KW IL R+ S+ GE+G T+
Sbjct: 83 TTRADGRVRLRIDPSCPQLPYTVHRMFYEALDKYGDLIALGFKRQDKWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILKIWKQLPHLKAVVIYKEPPPNKMANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKLVNQDAEGI-GEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTM 700 HSP 2 Score: 330.487 bits (846), Expect = 2.060e-96 Identity = 212/626 (33.87%), Postives = 321/626 (51.28%), Query Frame = 0
Query: 644 TTLPDGSVIIRRKKDDLQ-PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILW--RDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISK----LKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMP---------------------TTTDFFLS------------------LDIVIKVAYGLSEC-----GIFSDFAPSEYKKGTCGSI------------------FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTE-LSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
TT DG V +R Q P +V K G IA+ KR W +YSQYY R AAK F+KLGLK H+V+I FN PEW S +G +FAGG TG+Y ++S +Y+ + +++V+ L+K+ I + + HL VV+ +E +K++ ++ + +ELG E+ L + NQCC ++YTSGTTGNPKGVM+S D +T+TA+ + + ++ +VSYLPLSHIAA D++ I ++ FA+ ALKGSLV TL+EV PT GVPRV+EK E I+E ++ +K + ++ + + K F L + ++ K++ +LG +CQ+ G AP+M T+ F+S + +V + A G+ E IF + E K TC +I F GR KEL+ITAGGEN+ P+ IE+ +K LP++SN + IGD +KF+S+ LT K D + T+ L+ E+C +G A+TV+ II + V AI++GI N + ++KW+++ R+ S+ GE+GPT+KLKR
Sbjct: 83 TTRADGRVRLRIDPSCPQLPYTVHRMFYEALDKYGDLIALGFKRQDKWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILKIWKQLPHLKAVVIYKEPP--PNKMANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKLVNQDAEGIGEICLWGRTIFMGYLNMEDK--TCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTMKLKR 704 HSP 3 Score: 105.916 bits (263), Expect = 8.423e-22 Identity = 51/134 (38.06%), Postives = 83/134 (61.94%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTE-LSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+ IE+ +K LP++SN + IGD +KF+S+ LT K D + T+ L+ ++C + A+TV+ II K V AI++GI + N A + ++KW+++ R+ S+ GE+GPT+
Sbjct: 567 KELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTM 700
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: gb|EFA11034.1| (Very long-chain-fatty-acid--CoA ligase bubblegum-like Protein [Tribolium castaneum]) HSP 1 Score: 474.937 bits (1221), Expect = 1.397e-150 Identity = 265/647 (40.96%), Postives = 375/647 (57.96%), Query Frame = 0
Query: 1 MEAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIR-TESSEFLS----FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVI-----HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP-PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
M +E N D++ P A TTT PD V +R E+ + + S+P ++K ALA G W K Y +YY ++ AKAFIKLGL+ + VCI FN PEWFIS+L AIFAGG++ G+Y TNSS + N+SR I+VV++ + L I I + LK I I+ +G ++I W+DLL +GR + + L L I VN+CC+L+YTSGT G PK VMLSHD +T+ +I ++ D + ++VSYLPLSH+AA DIY+A+ +YFAD ALKGSL++TL+E +PT F GVPRVWEK E + + + + + +A K ++HY+ N + V + + LIF+KVKQALGL RC F+S APL+ + +FLS+D+P+ E +G+SE G F T G +P K++ D GEIC+ GR V MGYL + + T EA D EG+ H TGRLK+L++T+GGEN+ P PIE +K LP VSN +VGD KF+SV LT K + D P L SV+ K+LGC TV E+L P P +++A+++ ID N++A+SNA +++K ILP + S+ GE+G T+
Sbjct: 1 MASEFQNG-PDQILP-----AEDSTTTSPDVPVRLRIPETGKAIETTPPVSIPGLLKRTATDFPDHPALAYKAEGKWNKITYKEYYELVRTCAKAFIKLGLERHHAVCILGFNSPEWFISDLAAIFAGGIAVGIYTTNSSEACFHCANHSRANIIVVQDQKQLEKILAIKSRLPHLKAI--IQYEGTPTSADVISWEDLLIIGRDQEDEDLERVLKTIGVNECCTLVYTSGTVGKPKAVMLSHDNLTWDALSICERLDVKKGEEVIVSYLPLSHVAAQVTDIYIAMMYAVCIYFADANALKGSLINTLQEAQPTKFLGVPRVWEKMHEKMMQVAAQNGRVKKSLASWAKSQALHYHLNRIKGVNGSGWGYCLASSLIFKKVKQALGLSRCTLFVSAAAPLAADIKRYFLSIDMPIMEAFGMSEASGAHTMCNMGSFGLETIGMALPGMKTKILNPEDGQ--GEICMNGRHVFMGYLGDREKTDEAIDSEGWLHSGDLGRIDENNFIYITGRLKELIITAGGENIPPVPIEQLVKSELPHVSNVFLVGDKRKFLSVLLTFKTDVDSDTARPLDTLLPSVQDWLKSLGCPAKTVTEVLEAGPHPKLLEALKESIDRVNQQAVSNAQRIQKLSILPVDFSVATGELGPTM 637 HSP 2 Score: 350.903 bits (899), Expect = 8.182e-105 Identity = 222/644 (34.47%), Postives = 340/644 (52.80%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR-----RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN-----LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQ----------------GR-----------KKELLITAGG-----------EN--------IAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFL------------TFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNI 1205
++I A++ TTT PD V +R + + P+S+P +K A+A K G W + TY +YY +R AKAFIKLGL+ H V I FN PEW IS+L AIFAGG A G+Y +NS+ + N+SR I+VV++ L+K+ I + HL ++ E AD +S W DL+ +G+ +E++ L++ L I N+CC+++YTSGT G PK VM+SHD LT+ A ++ E+ K + IVSYLPLSH+AA DIY+ + +YFAD +ALKGSL+ TL+E +PT F GVPRV+EK E + + ++ + +K + + K A Y+ K + L + +++KK+K +LGL +C F APL +FLS+D+ I A+G+SE G + + T G P + GR K + I + G EN + ++I +N PV + +L +VFL TFK + D++ +P L SV++W SLG A TVT ++ P P+++ A+++ ID N+ VSNA R++K S++P + S+ GE+GPT+K+KRN + + +I
Sbjct: 10 DQILPAEDSTTTSPDVPVRLRIPETGKAIETTPPVSIPGLLKRTATDFPDHPALAYKAEGKWNKITYKEYYELVRTCAKAFIKLGLERHHAVCILGFNSPEWFISDLAAIFAGGIAVGIYTTNSSEACFHCANHSRANIIVVQDQKQLEKILAIKSRLPHLKAIIQYEGTPTSADVIS--WEDLLIIGRDQEDEDLERVLKTIGVNECCTLVYTSGTVGKPKAVMLSHDNLTWDALSICERLDVKKGEEVIVSYLPLSHVAAQVTDIYIAMMYAVCIYFADANALKGSLINTLQEAQPTKFLGVPRVWEKMHEKMMQVAAQNGRVKKSLASWAKSQALHYHLNRIKGVNGSGWGYCLASSLIFKKVKQALGLSRCTLFVSAAAPLAADIKRYFLSIDMPIMEAFGMSEASGAHTMCNMGSFGLETIGMALPGMKTKILNPEDGQGEICMNGRHVFMGYLGDREKTDEAIDSEGWLHSGDLGRIDENNFIYITGRLKELIITAGGENIPPVPIEQLVKSELPHVSNVFLVGDKRKFLSVLLTFKTDVDSDTARPLDTLLPSVQDWLKSLGCPAKTVTEVLEAGPHPKLLEALKESIDRVNQQAVSNAQRIQKLSILPVDFSVATGELGPTMKVKRNVVAAKYADI 651 HSP 3 Score: 113.235 bits (282), Expect = 1.560e-24 Identity = 59/135 (43.70%), Postives = 90/135 (66.67%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ IE +K+ LP +SNV +GD +KF+SV LTFK + D + +P L V+DW SL A TVT ++ P +++ A+++ ID+ N+ AVSNA +++K S++P + S+ GE+GPT+
Sbjct: 503 KELIITAGGENIPPVPIEQLVKSELPHVSNVFLVGDKRKFLSVLLTFKTDVDSDTARPLDTLLPSVQDWLKSLGCPAKTVTEVLEAGPHPKLLEALKESIDRVNQQAVSNAQRIQKLSILPVDFSVATGELGPTM 637
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: EFX85557.1 (hypothetical protein DAPPUDRAFT_187637 [Daphnia pulex]) HSP 1 Score: 430.254 bits (1105), Expect = 5.391e-134 Identity = 243/618 (39.32%), Postives = 350/618 (56.63%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHK-----IGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQK--SDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYT-TVGEILYHKPPGV-------------------MKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
SVP +++ AL + R+G W+KW Y QY D+++ AKAFI+LGL+ F++VCI FN PEW I++L AI AGG +AG+Y TNS+ + N + I+VVE+ + L I I + TLK IV + G E +I W L+ LG + DRL ++VNQCC+LIYTSGTTG PKGVML+HD +T+++ + + K +SYLPLSH+AA DIY L + G VYFAD LKG+L +TL+EVRPT FFGVPRVWEK E ++ K + +A K + Y + K + + L+F+KV++ LGLD+ +SG APLS+ ++F SLDIP+ E YG+SE S+ ++ + G +P K+ + D D GEI + GR V MGYL + TS D +GY + TGR+K++L+T+GGENVAP IE+ IK LP+VS+A+VVGD KF+S+ LT++ + + D EP LT + ++ G + TV +++ G+ +K I I++AN+ AIS A +V+KW ILP + S+ GE+G T+
Sbjct: 7 LSVPTLLQRTVARHMDHPALCVKRDGKWIKWTYKQYLHDVRVCAKAFIRLGLERFHSVCILGFNSPEWIIADLAAIHAGGFAAGIYTTNSAEACLHCALNGQADIIVVEDRKQLEKILSIKDQIPTLKAIV--QYTGKPHVEGVITWSQLMHLGNATPDNVYEDRLKKLAVNQCCTLIYTSGTTGPPKGVMLNHDNLTWISHNLATYMNLRDGKDTFLSYLPLSHVAAQITDIYAPLSVGGTVYFADPDVLKGTLAETLREVRPTCFFGVPRVWEKIYEKMQAIGKQTTGIKKTIATWSKSVGLSYNRRRMEGRSSKPFGFALANALVFKKVREGLGLDKARVILSGAAPLSKEVAEYFTSLDIPILEVYGMSESTGPHSINNITKGYQLNSAGKSVPGCLTKIA-NPDKDGNGEILMGGRHVFMGYLNDPGNTSSVVDADGYLSTGDVGHTDKFGFVYITGRIKEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDFCESYGLSLAETVTDVICAAERGMAERDPMAALTGQDAEAVKFVKVIDAAIEKANRNAISAAQRVQKWTILPVDFSIPGGELGPTM 621 HSP 2 Score: 300.056 bits (767), Expect = 7.010e-87 Identity = 155/377 (41.11%), Postives = 223/377 (59.15%), Query Frame = 0
Query: 660 LQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNT-----TGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE 1030
+ P+SVP+ ++ + A+ +KR+G W++WTY QY ++R+ AKAFI+LGL+ FH+V I FN PEW+I++L AI AGGFA G+Y +NSA + N + I+VVE+ L+K+ I + I L +V + G + I W L+ LG ++ + RL +A NQCC++IYTSGTTG PKGVM++HD LT+ + + K + +SYLPLSH+AA DIY + G +YFAD LKG+L TL+EVRPT FFGVPRV+EK E ++ + +K I K YN +K F L N +++KK+++ LGLD+ + G APL ++F SLDI I YG+SE
Sbjct: 4 IPPLSVPTLLQRTVARHMDHPALCVKRDGKWIKWTYKQYLHDVRVCAKAFIRLGLERFHSVCILGFNSPEWIIADLAAIHAGGFAAGIYTTNSAEACLHCALNGQADIIVVEDRKQLEKILSIKDQIPTLKAIV--QYTGKPHVEGVITWSQLMHLGNATPDNVYEDRLKKLAVNQCCTLIYTSGTTGPPKGVMLNHDNLTWISHNLATYMNLRDGKDTFLSYLPLSHVAAQITDIYAPLSVGGTVYFADPDVLKGTLAETLREVRPTCFFGVPRVWEKIYEKMQAIGKQTTGIKKTIATWSKSVGLSYNRRRMEGRSSKPFGFALANALVFKKVREGLGLDKARVILSGAAPLSKEVAEYFTSLDIPILEVYGMSE 378 HSP 3 Score: 122.865 bits (307), Expect = 1.494e-27 Identity = 67/176 (38.07%), Postives = 105/176 (59.66%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQ-PSTELSNSVKEWCHSLGYE-ASTVTNII---------RD----------QPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNI 1205
F GR KE+LITAGGEN+AP+LIEDNIK LP++S+ + +GD +KF+S+ LT + D + Q P L+ +++C S G A TVT++I RD + + + I I+ AN +S A RV+KW+++P + S+ GE+GPT+K+KR+ +K + ++
Sbjct: 460 FVYITGRIKEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDFCESYGLSLAETVTDVICAAERGMAERDPMAALTGQDAEAVKFVKVIDAAIEKANRNAISAAQRVQKWTILPVDFSIPGGELGPTMKMKRSCVAKKYADV 635 HSP 4 Score: 106.301 bits (264), Expect = 2.037e-22 Identity = 57/154 (37.01%), Postives = 92/154 (59.74%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQ-PSTELSNLVKDWCHSLSYE-ASTVTNII---------RD----------KPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+AP+LIED IK LP++S+ + +GD +KF+S+ LT + D + Q P L+ +D+C S A TVT++I RD + ++ + I I+KAN+ A+S A +V+KW+++P + S+ GE+GPT+
Sbjct: 468 KEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDFCESYGLSLAETVTDVICAAERGMAERDPMAALTGQDAEAVKFVKVIDAAIEKANRNAISAAQRVQKWTILPVDFSIPGGELGPTM 621
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: EAA10184.3 (AGAP008596-PA [Anopheles gambiae str. PEST]) HSP 1 Score: 429.869 bits (1104), Expect = 2.954e-133 Identity = 248/658 (37.69%), Postives = 367/658 (55.78%), Query Frame = 0
Query: 21 ASSYTTTKPDKGVHIRTESSEFLSF---SVPNMVKNHCLAGGSKTALAIMRNGN--WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIV---------VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK-HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASM-HYYENGDQKVI----HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSD-FKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP----------------------PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A SYT+T P V +R S SVP+++ N + + RN W Y +Y + AK FIKLGL+P +TV + +FN PEWF+S L AI AGG+ G+Y TNS+ + ++++ +SR QI+VV++ + + I I +N+ LK ++ V +DGF W +L E+ + + N RL++I++NQCC L+YTSGT GNPKGVMLSHD T+ + AI K+ H A+ ++VS+LPLSH+AA +DI++ L VYFADK A+KG+L+ TL+E +PT VPRV+EK QE + ++ VA K ++ H+ + + K +++ I KVK ALG RCL + AP+ T+ +F+SLD+P+ E +G+SE S+ D + T G + K+ K D+ GEIC++GR V+MGY+ E T EA D +G+ H TGR+K+L++T+GGEN+ P +EN +K+ LP VSNA +VGD KF+++ +T+K + N D GEP ELT K LG Y + EI P GV+KA+Q+GID AN++AISNA K++K+ +L + S+ GE+G TL
Sbjct: 21 ADSYTSTDPAVPVKLRIAKEGLASLDPLSVPDLM-NRTVRDHPDHPAMVFRNAQKQWQTVTYREYRERVHHMAKVFIKLGLEPHHTVAVLAFNSPEWFVSELSAIHAGGIITGVYTTNSAESVQHVLESSRAQIVVVDDAKQMEKICSIRQNLPHLKTVIQTMPPYAPYVKRDDGF------YRWSELEEMNVDDVEEEFNRRLANIAINQCCCLVYTSGTVGNPKGVMLSHDNFTWDSYAIGKRLHQIRYAEEVLVSFLPLSHVAAQMVDIFLTLQFACTVYFADKDAMKGTLLHTLQEAKPTRMLAVPRVYEKIQEKMLAIGAQSGAAKKLVAGWAKSVTLQHHLDAMEGKPTNSWQYRLVKNYILSKVKDALGFSRCLTLATAAAPMDRETKKYFMSLDLPIMEAFGMSETSGAHSLTAPDSYNFDTIGKPLGGCETKIDK-PDERGHGEICMRGRHVLMGYIGEEGKTREAVDDDGWMHSGDVGYLDEAGFMYITGRIKELIITAGGENIPPVHVENLVKNELPYVSNAFLVGDKRKFLTMLITLKTQMNLDSGEPKDELTPETIAALKELGVEYGKLSEIHAAGPCPKVAFYFLGYWMTCSTQPFLGAGVLKALQEGIDRANQKAISNAQKIQKFALLKSDFSVPGGELGPTL 670 HSP 2 Score: 338.961 bits (868), Expect = 3.747e-100 Identity = 216/667 (32.38%), Postives = 331/667 (49.63%), Query Frame = 0
Query: 633 TEKIEDAKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKR-NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV---------VIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKIS--IVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYK-----KIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIF---------PSTQG------RKKELLITAGGE--------------------------------------------NIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PE---------------------VMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
T +I A YT+T P V +R K+ L P+SVP + AM + W TY +Y + AK FIKLGL+P HTV++ +FN PEW +S L AI AGG TG+Y +NSA ++++ +SR QI+VV++ ++K+ I +N+ HL V+ V +DGF W +L E+ + E++ +RL NIA NQCC ++YTSGT GNPKGVM+SHD T+ + + ++ + +VS+LPLSH+AA +DI++ + +YFADK A+KG+L+ TL+E +PT VPRVYEK QE + ++ ++K + K ++ + N L K K+KD+LG +C + AP+ T +F+SLD+ I A+G+SE G S AP Y T G P +G R + +L+ GE NI P+ +E+ +KN LP +SN +GD +KF+++ +T K + + G+P EL+ LG E ++ I P P+ V+ A+Q+GID AN+ +SNA +++K++L+ + S+ GE+GPTLK+KRN
Sbjct: 15 TGRILPADSYTSTDPAVPVKLRIAKEGLASLDPLSVPDLMNRTVRDHPDHPAMVFRNAQKQWQTVTYREYRERVHHMAKVFIKLGLEPHHTVAVLAFNSPEWFVSELSAIHAGGIITGVYTTNSAESVQHVLESSRAQIVVVDDAKQMEKICSIRQNLPHLKTVIQTMPPYAPYVKRDDGFYR------WSELEEMNVDDVEEEFNRRLANIAINQCCCLVYTSGTVGNPKGVMLSHDNFTWDSYAIGKRLHQIRYAEEVLVSFLPLSHVAAQMVDIFLTLQFACTVYFADKDAMKGTLLHTLQEAKPTRMLAVPRVYEKIQEKMLAIGAQSGAAKKLVAGWAKSVTLQHHLDAMEGKPTNSWQYRLVKNYILSKVKDALGFSRCLTLATAAAPMDRETKKYFMSLDLPIMEAFGMSETSGAHSLTAPDSYNFDTIGKPLGGCETKIDKPDERGHGEICMRGRHVLMGYIGEEGKTREAVDDDGWMHSGDVGYLDEAGFMYITGRIKELIITAGGENIPPVHVENLVKNELPYVSNAFLVGDKRKFLTMLITLKTQMNLDSGEPKDELTPETIAALKELGVEYGKLSEIHAAGPCPKVAFYFLGYWMTCSTQPFLGAGVLKALQEGIDRANQKAISNAQKIQKFALLKSDFSVPGGELGPTLKIKRN 675 HSP 3 Score: 92.4337 bits (228), Expect = 5.267e-18 Identity = 53/156 (33.97%), Postives = 85/156 (54.49%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLE----------------------VISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ +E+ +KN LP +SN +GD +KF+++ +T K + +G+P EL+ L E ++ I P V+ A+Q+GID+AN+ A+SNA K++K++L+ + S+ GE+GPTL
Sbjct: 515 KELIITAGGENIPPVHVENLVKNELPYVSNAFLVGDKRKFLTMLITLKTQMNLDSGEPKDELTPETIAALKELGVEYGKLSEIHAAGPCPKVAFYFLGYWMTCSTQPFLGAGVLKALQEGIDRANQKAISNAQKIQKFALLKSDFSVPGGELGPTL 670
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: XP_006571405.1 (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 isoform X2 [Apis mellifera]) HSP 1 Score: 428.328 bits (1100), Expect = 4.203e-133 Identity = 249/649 (38.37%), Postives = 374/649 (57.63%), Query Frame = 0
Query: 1 MEAEHSNSMTDKMFPNKQALASSYTTTKPDKGVHIR--TESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVK--WNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHD----IITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYEN----GDQKVIHKIGDK-LIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
M++E D++ P A S+ TT+ + V I+ E + S+P ++ N + AL + N ++ + Y +Y + ++ AKAF+KLGL+ ++ VCI FN PEW +NLGAI+AGG + G+Y TN+ ++ S+ I+VVEN + L I I N+ LK V+++ +G ++++ W DL+++G E +LN+ L I+VN+CC+L++TSGT GNPK VMLSHD + M+ A+ K + T +VSYLPLSH+AA +DIY LY+ G +YFAD+ ALKGSLV+TL RPT F GVPRVWEK E ++ + N + +A K +++Y N D+K I K L+F K++ ALGLDRC IS APLS + +FLSLDIP+ + YG+SEC G + K + G I + K+ + D + GEIC+ GR + MGYL Q T+E D +G+ H TGR+K+L++T+GGENV P IE + L ++SNA+++GD K++++ +T+K + N E G P T + K +++G TV E+L P V + I K I AN + IS+A +V+K+ ILP + S+ GE+G TL
Sbjct: 1 MKSETGLDGPDQILP-----ADSFKTTEHNGRVRIQLNNEDVNSMPISIPGLMSNTAKKYSNHLALVTRPDENGIRKTYTYSEYENQVRTVAKAFLKLGLERYHGVCILGFNSPEWLFANLGAIYAGGFAVGIYTTNNPEACQHCAIISKANIIVVENTKQLEKILQIKHNLPHLK--VIVQYEGKPEEKDILSWNDLIKIGEAESDVKLNEVLKTIAVNECCTLVFTSGTVGNPKAVMLSHDNFLSNVRSMSMALELKEGYET----LVSYLPLSHVAAQVVDIYFMLYIAGTIYFADQNALKGSLVNTLLVARPTTFVGVPRVWEKIYEKMQAVAANNGFIKTWIASWAKSQALYYNINKMNGNDRKHWSYIIAKCLVFNKIRAALGLDRCKMCISAAAPLSSEIKQYFLSLDIPILDAYGMSECSGAHTVNTPVNLKLNSVGRPIFGLNTKLY-NPDSNGEGEICMSGRHIFMGYLNEPQKTNEVLDADGWLHSGDLGKFDSDGFLYITGRIKELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAETGAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTL 637 HSP 2 Score: 349.747 bits (896), Expect = 1.928e-104 Identity = 220/647 (34.00%), Postives = 348/647 (53.79%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDLQ--PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMR--WTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGN--KKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGS-IF--------PSTQG-------------------RKKELLITAGG-------------------ENIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNI 1205
++I A + TT +G V I+ +D+ P+S+P + + K + +A+ + + + +R +TYS+Y ++R AKAF+KLGL+ +H V I FN PEW+ +NLGAI+AGGFA G+Y +N+ ++ S+ I+VVEN L+K+ I N+ HL V+++ +G +K + W DLI++G+ E + +L + L IA N+CC++++TSGT GNPK VM+SHD +++ K G+ ++VSYLPLSH+AA +DIY ++ G +YFAD++ALKGSLV TL RPT F GVPRV+EK E ++ + + +I + K A YN GN K + + +++ KI+ +LGLD+C+ APL +FLSLDI I AYG+SEC G + P K + G IF P + G +K ++ A G I ++I +N P+ LSN + IGD +K++++ +T K +N++T G P + + +W S+G + TV+ ++ P V I K I AN +S+A RV+K+ ++P + S+ GE+GPTLK+KRN K++ N+
Sbjct: 11 DQILPADSFKTTEHNGRVRIQLNNEDVNSMPISIPGLMSNTAKKYSNHLALVTRPDENGIRKTYTYSEYENQVRTVAKAFLKLGLERYHGVCILGFNSPEWLFANLGAIYAGGFAVGIYTTNNPEACQHCAIISKANIIVVENTKQLEKILQIKHNLPHL--KVIVQYEGKPEEKDILSWNDLIKIGEAESDVKLNEVLKTIAVNECCTLVFTSGTVGNPKAVMLSHDNFLSNVRSMSMALELKEGYE---TLVSYLPLSHVAAQVVDIYFMLYIAGTIYFADQNALKGSLVNTLLVARPTTFVGVPRVWEKIYEKMQAVAANNGFIKTWIASWAKSQALYYNINKMNGNDRKHWSYIIAKCLVFNKIRAALGLDRCKMCISAAAPLSSEIKQYFLSLDIPILDAYGMSECSGAHTVNTPVNLKLNSVGRPIFGLNTKLYNPDSNGEGEICMSGRHIFMGYLNEPQKTNEVLDADGWLHSGDLGKFDSDGFLYITGRIKELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAET-GAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTLKVKRNIVYKMYENL 651 HSP 3 Score: 87.4261 bits (215), Expect = 1.940e-16 Identity = 46/134 (34.33%), Postives = 76/134 (56.72%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVN-SDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ PI IE + L +LSN + IGD +K++++ +T K + + G P + W S+ + TV+ ++ V I K I +AN +S+A +V+K+ ++P + S+ GE+GPTL
Sbjct: 504 KELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAETGAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTL 637
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: XP_006571404.1 (PREDICTED: very long-chain-fatty-acid--CoA ligase bubblegum isoform X1 [Apis mellifera]) HSP 1 Score: 427.943 bits (1099), Expect = 1.316e-132 Identity = 246/635 (38.74%), Postives = 369/635 (58.11%), Query Frame = 0
Query: 15 PNKQALASSYTTTKPDKGVHIR--TESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVK--WNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHD----IITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYEN----GDQKVIHKIGDK-LIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
P++ A S+ TT+ + V I+ E + S+P ++ N + AL + N ++ + Y +Y + ++ AKAF+KLGL+ ++ VCI FN PEW +NLGAI+AGG + G+Y TN+ ++ S+ I+VVEN + L I I N+ LK V+++ +G ++++ W DL+++G E +LN+ L I+VN+CC+L++TSGT GNPK VMLSHD + M+ A+ K + T +VSYLPLSH+AA +DIY LY+ G +YFAD+ ALKGSLV+TL RPT F GVPRVWEK E ++ + N + +A K +++Y N D+K I K L+F K++ ALGLDRC IS APLS + +FLSLDIP+ + YG+SEC G + K + G I + K+ + D + GEIC+ GR + MGYL Q T+E D +G+ H TGR+K+L++T+GGENV P IE + L ++SNA+++GD K++++ +T+K + N E G P T + K +++G TV E+L P V + I K I AN + IS+A +V+K+ ILP + S+ GE+G TL
Sbjct: 40 PDQILPADSFKTTEHNGRVRIQLNNEDVNSMPISIPGLMSNTAKKYSNHLALVTRPDENGIRKTYTYSEYENQVRTVAKAFLKLGLERYHGVCILGFNSPEWLFANLGAIYAGGFAVGIYTTNNPEACQHCAIISKANIIVVENTKQLEKILQIKHNLPHLK--VIVQYEGKPEEKDILSWNDLIKIGEAESDVKLNEVLKTIAVNECCTLVFTSGTVGNPKAVMLSHDNFLSNVRSMSMALELKEGYET----LVSYLPLSHVAAQVVDIYFMLYIAGTIYFADQNALKGSLVNTLLVARPTTFVGVPRVWEKIYEKMQAVAANNGFIKTWIASWAKSQALYYNINKMNGNDRKHWSYIIAKCLVFNKIRAALGLDRCKMCISAAAPLSSEIKQYFLSLDIPILDAYGMSECSGAHTVNTPVNLKLNSVGRPIFGLNTKLY-NPDSNGEGEICMSGRHIFMGYLNEPQKTNEVLDADGWLHSGDLGKFDSDGFLYITGRIKELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAETGAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTL 667 HSP 2 Score: 349.747 bits (896), Expect = 4.804e-104 Identity = 220/647 (34.00%), Postives = 348/647 (53.79%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDLQ--PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMR--WTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN---TTGN--KKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGS-IF--------PSTQG-------------------RKKELLITAGG-------------------ENIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNI 1205
++I A + TT +G V I+ +D+ P+S+P + + K + +A+ + + + +R +TYS+Y ++R AKAF+KLGL+ +H V I FN PEW+ +NLGAI+AGGFA G+Y +N+ ++ S+ I+VVEN L+K+ I N+ HL V+++ +G +K + W DLI++G+ E + +L + L IA N+CC++++TSGT GNPK VM+SHD +++ K G+ ++VSYLPLSH+AA +DIY ++ G +YFAD++ALKGSLV TL RPT F GVPRV+EK E ++ + + +I + K A YN GN K + + +++ KI+ +LGLD+C+ APL +FLSLDI I AYG+SEC G + P K + G IF P + G +K ++ A G I ++I +N P+ LSN + IGD +K++++ +T K +N++T G P + + +W S+G + TV+ ++ P V I K I AN +S+A RV+K+ ++P + S+ GE+GPTLK+KRN K++ N+
Sbjct: 41 DQILPADSFKTTEHNGRVRIQLNNEDVNSMPISIPGLMSNTAKKYSNHLALVTRPDENGIRKTYTYSEYENQVRTVAKAFLKLGLERYHGVCILGFNSPEWLFANLGAIYAGGFAVGIYTTNNPEACQHCAIISKANIIVVENTKQLEKILQIKHNLPHL--KVIVQYEGKPEEKDILSWNDLIKIGEAESDVKLNEVLKTIAVNECCTLVFTSGTVGNPKAVMLSHDNFLSNVRSMSMALELKEGYE---TLVSYLPLSHVAAQVVDIYFMLYIAGTIYFADQNALKGSLVNTLLVARPTTFVGVPRVWEKIYEKMQAVAANNGFIKTWIASWAKSQALYYNINKMNGNDRKHWSYIIAKCLVFNKIRAALGLDRCKMCISAAAPLSSEIKQYFLSLDIPILDAYGMSECSGAHTVNTPVNLKLNSVGRPIFGLNTKLYNPDSNGEGEICMSGRHIFMGYLNEPQKTNEVLDADGWLHSGDLGKFDSDGFLYITGRIKELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAET-GAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTLKVKRNIVYKMYENL 681 HSP 3 Score: 87.4261 bits (215), Expect = 2.236e-16 Identity = 46/134 (34.33%), Postives = 76/134 (56.72%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ PI IE + L +LSN + IGD +K++++ +T K + + G P + W S+ + TV+ ++ V I K I +AN +S+A +V+K+ ++P + S+ GE+GPTL
Sbjct: 534 KELIITAGGENVPPIYIEQLVLAELSILSNALLIGDKRKYLTMLVTLKTEINAETGAPKDNFTLNTLKWLQSIGSTSKTVSEVLEKHDPFVYEEIDKAIKRANTKVISHAQRVQKFQILPHDFSIVTGELGPTL 667
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: AHN54437.1 (bubblegum, isoform C [Drosophila melanogaster]) HSP 1 Score: 418.698 bits (1075), Expect = 2.129e-129 Identity = 244/645 (37.83%), Postives = 364/645 (56.43%), Query Frame = 0
Query: 7 NSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGN-WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDG---FRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTT-AKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQK----VIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE---CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP-PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+++ ++ PN+ A +Y TT V IR + SVP ++K G AL N + Y QY + AKAFIKLGL+ ++V + +FNC EWF S +GAI A G+ AG+Y TNS+ ++++ +S QI+VV++ + + I I + LK + I+E + + W ++ + + D+ RL ++++N+CC L+YTSGT G PKGVMLSHD ITF + IVK D +VSYLPLSH+AA +DIY ++ G ++FADK ALKG+LV +L++ RPT F GVPRV+EKFQE + + + + +A K ++ +Y K +KI LI KVKQALG DR L S AP+S T+ +FLSLD+ + + +G+SE C C T G +P +K + + D + GE+CI+GR V MGY+ N++ T E+ D + + H TGR K++++T+GGEN+ P IEN IK L +SNA +VG+ K+++V +T+K + ++D GEP EL+ K+LG + TV +IL P P V K+I+ I ANK++ISNA KV+K+ ILP + S+ GE+G TL
Sbjct: 5 DALYNRPGPNRLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTIC--LPDSVGLNTIGKTLPGCESKFI-NKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646 HSP 2 Score: 277.715 bits (709), Expect = 6.487e-79 Identity = 160/444 (36.04%), Postives = 239/444 (53.83%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIK--RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE---------EDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW--TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK----FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGR 1057
++ A Y TT +V IR KD + +P+SVP +K G A+ K +NG + TY QY +++ AKAFIKLGL+ H+V + +FNC EW S +GAI A G G+Y +NSA ++++ +S QI+VV++ + K+ I + + L + I+ EDG+ W ++ + ++ EDQ RL N+A N+CC ++YTSGT G PKGVM+SHD +T+ + +V+ SIVSYLPLSH+AA +DIY G ++FADK ALKG+LV++L++ RPT F GVPRVYEKFQE + S +K + + K + K F + ++ K+K +LG D+ + AP+ P T +FLSLD+ I A+G+SE G + P T G P + +
Sbjct: 15 RLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNG-YHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYR------WSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESK 451 HSP 3 Score: 132.109 bits (331), Expect = 1.555e-30 Identity = 66/156 (42.31%), Postives = 103/156 (66.03%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFN 1204
+ S GR KE++ITAGGENI P+ IE+ IK L +SN +G+ +K+++V +T K D + G+P ELS+ W SLG E TV++I+ P P+V +I+ I AN+ +SNA +V+K++++P + S+ GE+GPTLK+KRN SK++ +
Sbjct: 504 YVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYAD 659 HSP 4 Score: 108.612 bits (270), Expect = 4.914e-23 Identity = 56/137 (40.88%), Postives = 89/137 (64.96%), Query Frame = 0
Query: 1312 KEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
+ K++ ITAG ENI P+ IE+ IK L +SN +G+ +K+++V +T K DK+ G+P ELS+ W SL E TV++I+ P +V +I+ I +ANK ++SNA KV+K++++P + S+ GE+GPTL
Sbjct: 510 RSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: AAF53368.1 (bubblegum, isoform A [Drosophila melanogaster]) HSP 1 Score: 418.698 bits (1075), Expect = 2.129e-129 Identity = 244/645 (37.83%), Postives = 364/645 (56.43%), Query Frame = 0
Query: 7 NSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGN-WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDG---FRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTT-AKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQK----VIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE---CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP-PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+++ ++ PN+ A +Y TT V IR + SVP ++K G AL N + Y QY + AKAFIKLGL+ ++V + +FNC EWF S +GAI A G+ AG+Y TNS+ ++++ +S QI+VV++ + + I I + LK + I+E + + W ++ + + D+ RL ++++N+CC L+YTSGT G PKGVMLSHD ITF + IVK D +VSYLPLSH+AA +DIY ++ G ++FADK ALKG+LV +L++ RPT F GVPRV+EKFQE + + + + +A K ++ +Y K +KI LI KVKQALG DR L S AP+S T+ +FLSLD+ + + +G+SE C C T G +P +K + + D + GE+CI+GR V MGY+ N++ T E+ D + + H TGR K++++T+GGEN+ P IEN IK L +SNA +VG+ K+++V +T+K + ++D GEP EL+ K+LG + TV +IL P P V K+I+ I ANK++ISNA KV+K+ ILP + S+ GE+G TL
Sbjct: 5 DALYNRPGPNRLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTIC--LPDSVGLNTIGKTLPGCESKFI-NKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646 HSP 2 Score: 277.715 bits (709), Expect = 6.487e-79 Identity = 160/444 (36.04%), Postives = 239/444 (53.83%), Query Frame = 0
Query: 635 KIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIK--RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE---------EDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW--TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK----FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGR 1057
++ A Y TT +V IR KD + +P+SVP +K G A+ K +NG + TY QY +++ AKAFIKLGL+ H+V + +FNC EW S +GAI A G G+Y +NSA ++++ +S QI+VV++ + K+ I + + L + I+ EDG+ W ++ + ++ EDQ RL N+A N+CC ++YTSGT G PKGVM+SHD +T+ + +V+ SIVSYLPLSH+AA +DIY G ++FADK ALKG+LV++L++ RPT F GVPRVYEKFQE + S +K + + K + K F + ++ K+K +LG D+ + AP+ P T +FLSLD+ I A+G+SE G + P T G P + +
Sbjct: 15 RLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNG-YHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYR------WSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESK 451 HSP 3 Score: 132.109 bits (331), Expect = 1.555e-30 Identity = 66/156 (42.31%), Postives = 103/156 (66.03%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFN 1204
+ S GR KE++ITAGGENI P+ IE+ IK L +SN +G+ +K+++V +T K D + G+P ELS+ W SLG E TV++I+ P P+V +I+ I AN+ +SNA +V+K++++P + S+ GE+GPTLK+KRN SK++ +
Sbjct: 504 YVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYAD 659 HSP 4 Score: 108.612 bits (270), Expect = 4.914e-23 Identity = 56/137 (40.88%), Postives = 89/137 (64.96%), Query Frame = 0
Query: 1312 KEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
+ K++ ITAG ENI P+ IE+ IK L +SN +G+ +K+++V +T K DK+ G+P ELS+ W SL E TV++I+ P +V +I+ I +ANK ++SNA KV+K++++P + S+ GE+GPTL
Sbjct: 510 RSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTL 646
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: EFX85329.1 (hypothetical protein DAPPUDRAFT_314088 [Daphnia pulex]) HSP 1 Score: 410.994 bits (1055), Expect = 6.086e-125 Identity = 231/579 (39.90%), Postives = 328/579 (56.65%), Query Frame = 0
Query: 7 NSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHK-----IGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQK--SDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLG 559
N D++FP A SYTTT V ++ + S SVP +++ AL + R+G W+KW Y QY D+++ AKAFI+LGL+ F++VCI FN PEW I++L AI AGG +AG+Y TNS+ + N + I+VVE+ + L I I + TLK IV + G E +I W L+ LG + DRL ++VNQCC+LIYTSGTTG PKGVML+HD +T+++ + + K +SYLPLSH+AA DIY L + G VYFAD LKG+L +TL+EVRPT FFGVPRVWEK E ++ K + +A K + Y + K + + L+F+KV++ LGLD+ +SG APLS+ ++F SLDIP + YG+SE S+ ++ + G +P K+ + D D GEI + GR V MGYL + TS D +GY + TGR+K++L+T+GGENVAP IE+ IK LP+VS+A+VVGD KF+S+ LT++ + + D EP LT + ++ G
Sbjct: 184 NKGPDQLFP-----ADSYTTTSATGAVKLKIDERGMASIPPLSVPTLLQRTVARHMDHPALCVKRDGKWIKWTYKQYLHDVRVCAKAFIRLGLERFHSVCILGFNSPEWIIADLAAIHAGGFAAGIYTTNSAEACLHCALNGQADIIVVEDRKQLEKILSIKDQIPTLKAIV--QYTGKPHVEGVITWSQLMHLGNATPDNVYEDRLKKLAVNQCCTLIYTSGTTGPPKGVMLNHDNLTWISNNLATYMNLRDGKDTFLSYLPLSHVAAQITDIYAPLSVGGTVYFADPDVLKGTLAETLREVRPTCFFGVPRVWEKIYEKMQAIGKQTTGIKKTIATWSKSVGLSYNRRRMEGRSSKPFGFALANALVFKKVREGLGLDKARVILSGAAPLSKEVAEYFTSLDIPPAQVYGMSESTGPHSINNITKGYQLNSAGKSVPGCLTKIA-NPDKDGNGEILMGGRHVFMGYLNDPGNTSSVVDADGYLSTGDVGHTDKFGFVYITGRIKEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDICESYG 754 HSP 2 Score: 306.22 bits (783), Expect = 1.408e-87 Identity = 163/401 (40.65%), Postives = 232/401 (57.86%), Query Frame = 0
Query: 639 AKEYTTTLPDGSV---IIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNT-----TGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE 1030
A YTTT G+V I R + P+SVP+ ++ + A+ +KR+G W++WTY QY ++R+ AKAFI+LGL+ FH+V I FN PEW+I++L AI AGGFA G+Y +NSA + N + I+VVE+ L+K+ I + I L +V + G + I W L+ LG ++ + RL +A NQCC++IYTSGTTG PKGVM++HD LT+ + + K + +SYLPLSH+AA DIY + G +YFAD LKG+L TL+EVRPT FFGVPRV+EK E ++ + +K I K YN +K F L N +++KK+++ LGLD+ + G APL ++F SLDI YG+SE
Sbjct: 193 ADSYTTTSATGAVKLKIDERGMASIPPLSVPTLLQRTVARHMDHPALCVKRDGKWIKWTYKQYLHDVRVCAKAFIRLGLERFHSVCILGFNSPEWIIADLAAIHAGGFAAGIYTTNSAEACLHCALNGQADIIVVEDRKQLEKILSIKDQIPTLKAIV--QYTGKPHVEGVITWSQLMHLGNATPDNVYEDRLKKLAVNQCCTLIYTSGTTGPPKGVMLNHDNLTWISNNLATYMNLRDGKDTFLSYLPLSHVAAQITDIYAPLSVGGTVYFADPDVLKGTLAETLREVRPTCFFGVPRVWEKIYEKMQAIGKQTTGIKKTIATWSKSVGLSYNRRRMEGRSSKPFGFALANALVFKKVREGLGLDKARVILSGAAPLSKEVAEYFTSLDIPPAQVYGMSE 591 HSP 3 Score: 87.0409 bits (214), Expect = 3.345e-16 Identity = 43/88 (48.86%), Postives = 61/88 (69.32%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQ-PSTELSNSVKEWCHSLGYE-ASTVTNII 1141
GR KE+LITAGGEN+AP+LIEDNIK LP++S+ + +GD +KF+S+ LT + D + Q P L+ ++ C S G A TVT++I
Sbjct: 678 GRIKEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDICESYGLSLAETVTDVI 765 HSP 4 Score: 73.559 bits (179), Expect = 4.153e-12 Identity = 37/85 (43.53%), Postives = 55/85 (64.71%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQ-PSTELSNLVKDWCHSLSYE-ASTVTNII 1396
K++ ITAG EN+AP+LIED IK LP++S+ + +GD +KF+S+ LT + D + Q P L+ +D C S A TVT++I
Sbjct: 681 KEILITAGGENVAPVLIEDNIKAELPIVSHAMVVGDRRKFLSILLTLRTEVDPDTQEPLPILTRPTRDICESYGLSLAETVTDVI 765
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: EEB10392.1 (Long-chain-fatty-acid--CoA ligase, putative [Pediculus humanus corporis]) HSP 1 Score: 399.053 bits (1024), Expect = 2.570e-122 Identity = 238/639 (37.25%), Postives = 358/639 (56.03%), Query Frame = 0
Query: 21 ASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKW---NYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVEN---LQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENG--DQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKS-DFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPG-VMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFIS 627
+ Y ++K D V IR S SVP +++ ALA R+ + ++++YY K+ AK F+KLGL+ ++V + FN PEW I+ LG+I AGG+ G+Y TNS+ Y + + I VV+N Q + H L N+ +I+ G +N++ W++ +++G + ++L++ I+VN+CC++I+TSGTTGNPKGVMLSHD + + ++ D + + I+ SYLPLSH+AA +D+Y L ++ VYFA+ ALKG LV TL EVRPT F GVPRVWEK + IK + ++ K+ + NG + + + LIF+K+K LG D C F SG APLSE + + SLDI + E YG+SE +V + FK + G +P T K+ + D + GEI + GR + MGYL + + T E D +G+ H TGR K+LL+T+GGEN+ PA IE +K LP + + V++ D +KF+S+ +T + + D GEP +L++ K K+L YT EIL P ++++IQ+GIDEANK+AISNA K++K+ ILPR+ S GE+G TL FI+
Sbjct: 14 SDKYVSSKMDSPVKIREGIDRISSTSPLSVPTLLRKASSVAPKHPALAFKRHPKERSFCFISFEEYYRLAKVAAKGFLKLGLERHHSVALIGFNAPEWVIAYLGSILAGGVGCGIYSTNSAEATLYCLQAASVDIAVVDNNIQAQKILQYKHRLPNLKA-----IIQYFGRPLVQNVLSWREFIKIGEAQSSEKLHEVRKTIAVNECCTIIFTSGTTGNPKGVMLSHDNLIWNAYSVAINQDLISTEKII-SYLPLSHVAALMVDVYCPLMVRATVYFAETDALKGGLVRTLLEVRPTRFLGVPRVWEKVSDKIKLAGAESNFIKRRIVAWAKNQGIQRNLDLINGIDSPNLKYLLAKSLIFKKIKSRLGFDACTTFWSGAAPLSEGVKLYLTSLDIVILEVYGMSESSGPHTVNTNVAFKLDSVGKTLPGTMTKIY-NPDSNGEGEILLNGRNIFMGYLNDLKQTEEVVDPDGWLHTGDIGKFDDQGFLYITGRKKELLITAGGENIYPAVIEQNVKAQLPFLGHVVLICDKKKFLSILITFSSEEDVDTGEPLDQLSELTIKWLKDLNLTYTKNSEILTSGPHSKILESIQRGIDEANKKAISNAQKIQKFRILPRDFSTITGELGPTLKLKRNFIN 645 HSP 2 Score: 325.865 bits (834), Expect = 7.179e-96 Identity = 212/633 (33.49%), Postives = 318/633 (50.24%), Query Frame = 0
Query: 639 AKEYTTTLPDGSVIIRRKKDDLQ---PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRW---TYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYN-------TTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGR------KKELLITAGGENI-----------------------------------------APILIEDNIKNFLPVL--SNVVA----------IGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
+ +Y ++ D V IR D + P+SVP+ ++ A+A KR+ + ++ +YYR ++AAK F+KLGL+ H+V++ FN PEW+I+ LG+I AGG G+Y +NSA Y + + I VV+NN QK+ + +L ++ + + LS WR+ I++G+ + ++L + IA N+CC+II+TSGTTGNPKGVM+SHD L + A +V I+SYLPLSH+AA +D+Y + R +YFA+ ALKG LVRTL EVRPT F GVPRV+EK + I+ ++ ++ I K+ N + N K++ L +++KKIK LG D C F G APL + SLDIVI YG+SE G + +K + G P T + E I G NI +LI +N P + NV A I D KKF+S+ +TF D + G+P +LS +W L + + I+ P +++ +IQ+GID AN+ +SNA +++K+ ++PR+ S GE+GPTLKLKRN
Sbjct: 14 SDKYVSSKMDSPVKIREGIDRISSTSPLSVPTLLRKASSVAPKHPALAFKRHPKERSFCFISFEEYYRLAKVAAKGFLKLGLERHHSVALIGFNAPEWVIAYLGSILAGGVGCGIYSTNSAEATLYCLQAASVDIAVVDNNIQAQKILQYKHRLPNLKAIIQYFGRPLVQNVLS--WREFIKIGEAQSSEKLHEVRKTIAVNECCTIIFTSGTTGNPKGVMLSHDNLIWNAYSVAINQDLISTEKIISYLPLSHVAALMVDVYCPLMVRATVYFAETDALKGGLVRTLLEVRPTRFLGVPRVWEKVSDKIKLAGAESNFIKRRIVAWAKNQGIQRNLDLINGIDSPNLKYL--LAKSLIFKKIKSRLGFDACTTFWSGAAPLSEGVKLYLTSLDIVILEVYGMSESSGPHTVNTNVAFKLDSVGKTLPGTMTKIYNPDSNGEGEILLNGRNIFMGYLNDLKQTEEVVDPDGWLHTGDIGKFDDQGFLYITGRKKELLITAGGENIYPAVIEQNVKAQLPFLGHVVLICDKKKFLSILITFSSEEDVDTGEPLDQLSELTIKWLKDLNLTYTKNSEILTSGPHSKILESIQRGIDEANKKAISNAQKIQKFRILPRDFSTITGELGPTLKLKRN 642 HSP 3 Score: 114.005 bits (284), Expect = 8.960e-25 Identity = 65/150 (43.33%), Postives = 93/150 (62.00%), Query Frame = 0
Query: 1299 QCFRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPL-EVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
Q F Y+ GRK K+L ITAG ENI P +IE +K LP L +VV I D KKF+S+ +TF D G+P +LS L W L+ + + I+ P +++ +IQ+GID+ANK A+SNA K++K+ ++PR+ S GE+GPTL
Sbjct: 493 QGFLYIT----GRK-KELLITAGGENIYPAVIEQNVKAQLPFLGHVVLICDKKKFLSILITFSSEEDVDTGEPLDQLSELTIKWLKDLNLTYTKNSEILTSGPHSKILESIQRGIDEANKKAISNAQKIQKFRILPRDFSTITGELGPTL 637
BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Match: AAF53370.1 (heimdall [Drosophila melanogaster]) HSP 1 Score: 330.102 bits (845), Expect = 4.773e-97 Identity = 219/662 (33.08%), Postives = 334/662 (50.45%), Query Frame = 0
Query: 21 ASSYTTTKPDKGVHIRTESSEFLSFSVP-----NMVKNHCLAGGSKTALAIMRNGN----WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEE--DGF---RPGENLILWKDLLELG-RKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTK-AIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEG-IKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDK------LIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC--GIFCSVQKSDFKTG--TCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEIL--------------------YHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A+SY +T V +R + S +VP K C AL G+ W + +Y ++ A + +G++ +V I +FNCPEWF + GA+ AG + AG+YP+NS+ +++ + VV++ Q + + I + + LK ++ + + F PG W+ L E E + L R S I N+C LI+TSGT G PK VMLSHD + F TK A D K VSYLPLSH+AA D+++ L G V FADK ALKG+L+ T ++ RPT FGVPRV+EK QE + ++K + S++ +A+ + H K G+ + + +++ +G+D C F +GGAP SE + FFL LDI + ECYG+SE I +V S+ + C + H D + GEI ++GR V MGYL T E ++G+ H +GRLK+L++T+GGEN+ P IE IK LP VSN +++GD K+++V L++K K + + G P L + + ++L + T + E+L P +++AI++GI ANK AISNA KV+K+ ++ E S+ GE+G TL
Sbjct: 7 ATSYRSTSLHDAVKLRLDEPSSFSQTVPPQTIPEFFKESCEKYSDLPALVWETPGSGNDGWTTLTFGEYQERVEQAALMLLSVGVEERSSVGILAFNCPEWFFAEFGALRAGAVVAGVYPSNSAEAVHHVLATGESSVCVVDDAQQMAKLRAIKERLPRLKAVIQLHGPFEAFVDHEPG--YFSWQKLQEQTFSSELKEELLARESRIRANECAMLIFTSGTVGMPKAVMLSHDNLVFDTKSAAAHMQDIQVGKESFVSYLPLSHVAAQIFDVFLGLSHAGCVTFADKDALKGTLIKTFRKARPTKMFGVPRVFEKLQERLVAAEAKARPYSRLLLARARAAVAEHQTTLMAGKSPSIYGNAKYWLACRVVKPIREMIGVDNCRVFFTGGAPTSEELKQFFLGLDIALGECYGMSETSGAITLNVDISNLYSAGQACEGVTLKIH-----EPDCNGQGEILMRGRLVFMGYLGLPDKTEETVKEDGWLHSGDLGYIDPKGNLIISGRLKELIITAGGENIPPVHIEELIKKELPCVSNVLLIGDHRKYLTVLLSLKTKCDAKTGIPLDALREETIEWLRDLDIHETRLSELLNIPADLQLPNDTAALAATLEITAKPKLLEAIEEGIKRANKYAISNAQKVQKFALIAHEFSVATGELGPTL 661 HSP 2 Score: 219.55 bits (558), Expect = 4.199e-59 Identity = 133/395 (33.67%), Postives = 205/395 (51.90%), Query Frame = 0
Query: 662 PMSVPSFVKHNCLKGGSQIAMAIKRNGD----WMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV---------VIEEDGFIADKLSILWRDLIELG-KTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE--KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT----------GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE 1030
P ++P F K +C K A+ + G W T+ +Y + AA + +G++ +V I +FNCPEW + GA+ AG G+YPSNSA +++ + VV++ + K++ I E + L V+ V E G+ + W+ L E +E +++L R I AN+C +I+TSGT G PK VM+SHD L + K+ + K S VSYLPLSH+AA D+++ + G + FADK ALKG+L++T ++ RPT FGVPRV+EK QE + +K + + + + A + TT GN K+ L +++ K I++ +G+D C+ F GGAP FFL LDI + YG+SE
Sbjct: 35 PQTIPEFFKESCEKYSDLPALVWETPGSGNDGWTTLTFGEYQERVEQAALMLLSVGVEERSSVGILAFNCPEWFFAEFGALRAGAVVAGVYPSNSAEAVHHVLATGESSVCVVDDAQQMAKLRAIKERLPRLKAVIQLHGPFEAFVDHEPGYFS------WQKLQEQTFSSELKEELLARESRIRANECAMLIFTSGTVGMPKAVMLSHDNLVFDTKSAAAHMQDIQVGKESFVSYLPLSHVAAQIFDVFLGLSHAGCVTFADKDALKGTLIKTFRKARPTKMFGVPRVFEKLQERLVAAEAKARPYSRLLLARARAAVAEHQTTLMAGKSPSIYGNAKYW--LACRVV-KPIREMIGVDNCRVFFTGGAPTSEELKQFFLGLDIALGECYGMSE 420 HSP 3 Score: 114.005 bits (284), Expect = 9.919e-25 Identity = 70/177 (39.55%), Postives = 96/177 (54.24%), Query Frame = 0
Query: 1045 GTCGSIFPS----TQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDT-NGQPSTELSNSVKEWCHSLGYEA---STVTNIIRD-----------------QPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
G G I P GR KEL+ITAGGENI P+ IE+ IK LP +SNV+ IGD +K+++V L+ K D G P L EW L S + NI D P+++ AI++GI AN+ +SNA +V+K++LI E S+ GE+GPTLK++RN
Sbjct: 490 GDLGYIDPKGNLIISGRLKELIITAGGENIPPVHIEELIKKELPCVSNVLLIGDHRKYLTVLLSLKTKCDAKTGIPLDALREETIEWLRDLDIHETRLSELLNIPADLQLPNDTAALAATLEITAKPKLLEAIEEGIKRANKYAISNAQKVQKFALIAHEFSVATGELGPTLKIRRN 666 HSP 4 Score: 100.908 bits (250), Expect = 1.447e-20 Identity = 60/155 (38.71%), Postives = 87/155 (56.13%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNI---------------------IRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ IE+ IK LP +SNV+ IGD +K+++V L+ K D K G P L +W L + ++ + I KP +++ AI++GI +ANK A+SNA KV+K++LI E S+ GE+GPTL
Sbjct: 508 KELIITAGGENIPPVHIEELIKKELPCVSNVLLIGDHRKYLTVLLSLKTKCDAKTGIPLDALREETIEWLRDLDIHETRLSELLNIPADLQLPNDTAALAATLEITAKP-KLLEAIEEGIKRANKYAISNAQKVQKFALIAHEFSVATGELGPTL 661
BLAST of EMLSAG00000003336 vs. nr
Match: gi|1108468593|emb|CRL03590.1| (CLUMA_CG016250, isoform A, partial [Clunio marinus]) HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0 Identity = 440/1309 (33.61%), Postives = 686/1309 (52.41%), Query Frame = 0
Query: 16 NKQALASSYTTTKPDKGVHIRTESSEFLS----FSVPNMVKNHCLAGGSKTALAIMR--NGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIV---------VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAI-VKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYEN-----GDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKT-GTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKP-PGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGIT--LVNNV---GFISSSVLITEKIE---DAKEYTTTLPDGSVIIRRKKDD----LQPMSVPSFVKHNCLKGGSQIAMAIKR--NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVI---------EEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTG-----NKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEYKK-GTCGS--------------------------IFPSTQGRKKELLITAGGE------------NIAPILIEDNIK---------NFLPVL------------SNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQP-PEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
N+ + + T+ K V I + ++ S +VP ++K GS TAL W Y +Y ++ AK FIKLGL+ V + +FN EWF+S L AI AGG+ AG+Y TNS+ + +++ +S+ I+VV++ + + I I + LK ++ V +DG+ W +L + + + R + I N+CCSLIYTSGT G PKGVMLSHD +T+ ++ V+ ++ K ++VSYLPLSH+A +D+Y+ L L G +YFAD+ ALKGSLV TL E +PT+F GVPRV+EK QE + + + K +++++ + + +KI KL+ KVK ALG R ++G AP+S T+ +FLSLD+P+ + YG+SE S+ D T G +P +K++ + D++ GEICIKGR V MGYL + T E D EG+ F TGR+K+L++T+GGEN+ P IEN +K P +SNA +VGD KF+++ +T+K + + +G P EL K + L + T+ E+L P P V+ AIQ+ I+ ANK ++SNA KV+K+ ILP + S+ GE+G T L N+ + S + + + ++ D K T P +V I +D + P++VP+ +K GS A+ K W TY +Y + AK FIKLGL+ V++ +FN EW +S L AI AGG G+Y +NS+ +++ S+ I+VV++ L+K++ I + + HL VV I E +G+ + W+++ L + E + ++R I N+CC +IYTSGT G PKGVM+SHD LTY A T++ + K + ++SYLPLSHIA LD+++ + G YFADK+ALKGS+ R + +V+PT+FFGVPR++EK QE + ++ ++ I + K +N + ++ K+K+ LGL +C+ G AP+ T +FLS+++ I A+G+SE P ++ T GS +F ++ + T E N I I IK N PVL SN +GD +KF+++ +T K D+ G P EL+ +W L + T++ ++ P P+V+ AIQ+ I+ AN+ VSNA +V+K++++P + S+ GE+GPT+KLKRN
Sbjct: 1793 NRMKETTEFRVTEKSKPVRILRDPNDRPSSVAPITVPALMKRTVDNYGSHTALMYKDEVTKAWKGITYKEYRDRVEKIAKVFIKLGLERHGVVAVLAFNSVEWFVSELAAIHAGGIIAGVYTTNSTESVHHILESSKANIVVVDDAKQMEKIHAIKDKLPHLKAVIQTLSPYAPYVKRDDGY------YRWSELEAIKTETVEEEYQRRQAEIVANECCSLIYTSGTVGKPKGVMLSHDNLTWDAYSVTVRLNELQMGKEVLVSYLPLSHVAGQMVDVYIPLTLAGTIYFADRDALKGSLVKTLVEAQPTLFMGVPRVFEKMQEKMLAVGAQSGALKRLIGSWAKSVTLNHHLDRMAGRPSNSIQYKIATKLVMSKVKFALGFSRIKFMVTGAAPMSVDTKKYFLSLDMPILDVYGMSETSGGHSLSSFDSPAFETSGKSLPGIKSKII-NKDENGHGEICIKGRHVFMGYLNELEKTMETLDDEGWLRTGDIGYIDNDGYIFITGRIKELIITAGGENIPPVLIENLVKSECPAISNAFLVGDKRKFLTMLVTLKTEMDSEGAPKDELAGETLKWLEGLEVKHKTLSEVLAAGPDPKVLHAIQEAINRANKSSVSNAQKVQKFAILPHDFSIPTGELGPTMKLKRNIVADMYKDSMIKMNDSLKVSNDIKNIRVTDPTKTVTILNDSNDRSSSVAPITVPALMKRTVDNYGSHTALMYKDEVTKAWKGITYKEYRDRVEKIAKVFIKLGLERHGVVAVLAFNSLEWFVSELAAIHAGGIIAGIYSTNSSDACLHILETSKANIVVVDDAIQLEKIRQIKDKLPHLKAVVNILSSSLDDLKESEGYWS------WKEIEILDTNDVEQEYQRRSSQIVPNECCCLIYTSGTVGMPKGVMLSHDNLTYEAFTLMTRFECQKGRERVISYLPLSHIAGQMLDVFLPMMFAGTTYFADKNALKGSIFRYIYKVKPTLFFGVPRIFEKLQEMMINNEAQSGALKRAIYSWAKRVTLEHNLNQMAGRPTASILSKFIEMLITNKVKNDLGLGECRYMFTGAAPMSVETKKYFLSMNMKIYDAFGMSESSGAHCICPEDFNSFETVGSELFGFKTKTIDQDENGHGEICIKGRHVFMGYLNELEKTMETLDDEGWLRTGDIGYIDNDGYIFITGRIKELIITAGGENIPPVLIENLVKSECPAISNAFLVGDKRKFLTMLVTLKTEMDSEGAPKDELAGETLKWLEGLEVKHKTLSEVLAAGPDPKVLHAIQEAINRANKSSVSNAQKVQKFAILPHDFSIPTGELGPTMKLKRN 3088 HSP 2 Score: 623.239 bits (1606), Expect = 0.000e+0 Identity = 388/1061 (36.57%), Postives = 579/1061 (54.57%), Query Frame = 0
Query: 36 RTESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKW---NYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIV---------VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAI-VKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYY-----ENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC---GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEA--------------FDKEGY-FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGC--NYTTVGEILYHKPP-GVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDD----LQPMSVPSFVKHNCLKGGSQIAMAIKR--NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV---------VIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK--KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE 1030
RT S E +VP ++K G+ TAL + ++ + KW Y + ++ AK FIKLGL TV + +FN EWF+S L AI AGG AG+Y TNS + +++ +SR I++V++ + + I I + LK +V + EDG+ W ++ L + +RLS I+ N+CC L+YTSGT G PKGVMLSHD T+ A+ + + ++VSYLPLSH+AA +DI++ L + G VYFADK ALKGSLV TL E RPT F VPRV+EK E + + S + + ++ ++ ++ + +K+ + KVK+ALGLDR FI+G APLS T++FFLSLDI + E +G+SE SV +S F+ T G IP K++ +D GEICI+GR V MGY+ + + T E D++GY F TGR+K+L++T+GGEN+ P IENA+K P +SNA +VGD KF++V LT++ + +G P EL K + + C Y + EIL P V K +Q +D ANK+AISNA KV+K+ ILP + S+ GE G T+ + +V++ ++++ E+ T V I R +D + P++VP+ +K GS A+ K W TY +Y + AK FIKLGL+ V++ +FN EW +S L AI AGG G+Y +NS +++ +S+ I+VV++ ++K+ I + + HL V+ V +DG+ W +L + E++ ++R I AN+CCS+IYTSGT G PKGVM+SHD LT+ A +V + + K +VSYLPLSH+A +D+Y+ + G +YFAD+ ALKGSLV+TL E +PT+F GVPRV+EK QE ++ I KS ++ + A N+ K + +++ K+K +LG + + G AP+ T +FLSLD+ I YG+SE
Sbjct: 1173 RTASVE--PITVPALMKRTVENYGNHTAL-MHKDESSKKWKGITYKELRDRVEKMAKVFIKLGLKRHGTVAVLAFNSVEWFVSELAAIHAGGQIAGVYTTNSVDSCLHVLESSRANIVIVDDPKQMEKIHAIKDQLKDLKAVVQTLSPYAEYIKNEDGY------WRWSEIENLNTDNVEEEYQNRLSSIAANECCCLVYTSGTVGKPKGVMLSHDNFTWDAYALSAHVSNLQMGREVLVSYLPLSHVAAQIMDIFMMLTVAGTVYFADKDALKGSLVKTLLEARPTNFMAVPRVYEKLHEKLAQISTESNTIKRMIGSWARNVTLEHHLTRMSGRSSNSIQYKLAKTMFTSKVKKALGLDRAKNFITGAAPLSRETKEFFLSLDIHIVEAFGMSETTGGHTMSSVDQSSFE--TIGRQIPGVQTKIINEGEDG-KGEICIRGRHVFMGYINDMEKTIETIDDDRWLRTGDTGYIDRDGYVFITGRIKELIITAGGENIPPILIENAVKQQCPAISNAFLVGDKRKFLTVLLTLRTLVDGEGIPCDELAPETIKWLEGISCGKKYKKLSEILAAGPDVTVNKELQNAVDCANKKAISNAQKVQKFAILPCDFSIPTGEFGPTMK-----LKRNVVVENRMKETTEFRVTEKSKPVRILRDPNDRPSSVAPITVPALMKRTVDNYGSHTALMYKDEVTKAWKGITYKEYRDRVEKIAKVFIKLGLERHGVVAVLAFNSVEWFVSELAAIHAGGIIAGVYTTNSTESVHHILESSKANIVVVDDAKQMEKIHAIKDKLPHLKAVIQTLSPYAPYVKRDDGYYR------WSELEAIKTETVEEEYQRRQAEIVANECCSLIYTSGTVGKPKGVMLSHDNLTWDAYSVTVRLNELQMGKEVLVSYLPLSHVAGQMVDVYIPLTLAGTIYFADRDALKGSLVKTLVEAQPTLFMGVPRVFEKMQEKMLAVGAQSGALKRLIGSWAKSVTLNHHLDRMAGRPSNSIQYK-----IATKLVMSKVKFALGFSRIKFMVTGAAPMSVDTKKYFLSLDMPILDVYGMSE 2205 HSP 3 Score: 113.235 bits (282), Expect = 2.940e-21 Identity = 54/134 (40.30%), Postives = 87/134 (64.93%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKP-LEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+LIE+ +K+ P +SN +GD +KF+++ +T K D G P EL+ W L + T++ ++ P +V+ AIQ+ I++ANK +VSNA KV+K++++P + S+ GE+GPT+
Sbjct: 2294 KELIITAGGENIPPVLIENLVKSECPAISNAFLVGDKRKFLTMLVTLKTEMDSEGAPKDELAGETLKWLEGLEVKHKTLSEVLAAGPDPKVLHAIQEAINRANKSSVSNAQKVQKFAILPHDFSIPTGELGPTM 2427 HSP 4 Score: 113.235 bits (282), Expect = 2.940e-21 Identity = 54/134 (40.30%), Postives = 87/134 (64.93%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKP-LEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+LIE+ +K+ P +SN +GD +KF+++ +T K D G P EL+ W L + T++ ++ P +V+ AIQ+ I++ANK +VSNA KV+K++++P + S+ GE+GPT+
Sbjct: 2950 KELIITAGGENIPPVLIENLVKSECPAISNAFLVGDKRKFLTMLVTLKTEMDSEGAPKDELAGETLKWLEGLEVKHKTLSEVLAAGPDPKVLHAIQEAINRANKSSVSNAQKVQKFAILPHDFSIPTGELGPTM 3083 HSP 5 Score: 106.301 bits (264), Expect = 3.647e-19 Identity = 61/144 (42.36%), Postives = 89/144 (61.81%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCH--SLGYEASTVTNIIRDQPP-EVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
GR KEL+ITAGGENI PILIE+ +K P +SN +GD +KF++V LT + D G P EL+ +W S G + ++ I+ P V +Q +D AN+ +SNA +V+K++++P + S+ GE GPT+KLKRN
Sbjct: 1645 GRIKELIITAGGENIPPILIENAVKQQCPAISNAFLVGDKRKFLTVLLTLRTLVDGEGIPCDELAPETIKWLEGISCGKKYKKLSEILAAGPDVTVNKELQNAVDCANKKAISNAQKVQKFAILPCDFSIPTGEFGPTMKLKRN 1788 HSP 6 Score: 88.9669 bits (219), Expect = 6.647e-14 Identity = 54/136 (39.71%), Postives = 81/136 (59.56%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLS--YEASTVTNIIRDKP-LEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI PILIE+ +K P +SN +GD +KF++V LT + D G P EL+ W +S + ++ I+ P + V +Q +D ANK A+SNA KV+K++++P + S+ GE GPT+
Sbjct: 1648 KELIITAGGENIPPILIENAVKQQCPAISNAFLVGDKRKFLTVLLTLRTLVDGEGIPCDELAPETIKWLEGISCGKKYKKLSEILAAGPDVTVNKELQNAVDCANKKAISNAQKVQKFAILPCDFSIPTGEFGPTM 1783
BLAST of EMLSAG00000003336 vs. nr
Match: gi|431922356|gb|ELK19447.1| (Long-chain-fatty-acid--CoA ligase ACSBG2, partial [Pteropus alecto]) HSP 1 Score: 685.256 bits (1767), Expect = 0.000e+0 Identity = 443/1301 (34.05%), Postives = 660/1301 (50.73%), Query Frame = 0
Query: 46 SVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVEN-LQVLRTISHILKNVTTLKKIVVIE-EDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKF---IVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKV--------AQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTY----TAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALK-------------------------------------------GSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN--------LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGR----------------------------------------------KKELLITAG----------GENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
++P + + G+ AL NW N++QYY + A+A IKLGL+ F+ V I FN EW I++LG I AGGLS G+Y TNS+ +Y++ ++ IL+VEN LQ+ + +S ++ TLK I+ + +NL W D +ELG +L+ ++ NQC L+YTSGTTGNPKGVMLSHD IT+ A+ + ++ A+ ++VSYLPLSHIAA LD++V + + +YFA ALKG+LVDTL+EV+PT+F GVPR+WEK QE IKE + + KV ++ K + YE V +++ L+F KV+ ALGL+RC +SG L T +FFLSLDIPV E YGLSE G + +++ +CG I+ S ++ + D GE+C+ GR V MGYL EQ T E D++G+ H +GR+K++++T+ GENV+P PIEN IK +P++S+A++VGD KF+S+ LT+K + + GEP +L K +++G TTV EI+ V AIQ+GID AN+ A S A +V+KW IL ++ S+ GE + Y TT DG V +R+ K + IA+ KR W TY + Y E R AAKAF++LGLK FH V I N EW+I+++GAI AG S + + + +S + VV+++ LQK+ I +KHL +V +E I +L L WR +EL + +D L + + + NQCC++IY TG PK +M+SHD +T+ TA+++ KH + +VSYLPLS++ A D++V I G LYFA AL+ GSLV TL+EV+PT F+GVP ++E+ + + K S+L + F + K A T ++ + L ++ + + LGL +C + G L T D+FLSLD+ + YGLSE GI + +++ +CG P T R +E L G GE I P IE+ +K ++P++ V +G ++ LT KVN+DT GQP L++ +C L ++ +++II D P VM I +GI AN S++AR+ KW+++ + S+ GE+G T KLKR +KI+
Sbjct: 45 TIPELFRESVNRFGTYPALVSKSTENWEILNFNQYYEACRKAARALIKLGLERFHGVGILGFNSAEWLIASLGTILAGGLSVGIYATNSAEACQYVITRAKVNILLVENDLQLQKILSIPQSSIETLKAIIQYKLPMNVHNKDNLYSWDDFMELGNSIPNSQLDQIIAGQKANQCAVLMYTSGTTGNPKGVMLSHDNITWTAGAVSRDCGFSHAREKQEVLVSYLPLSHIAAQMLDVWVHMKIGAVMYFAQPDALKGTLVDTLQEVKPTIFLGVPRIWEKMQERIKENGTRDSQLKKKVFSWARAIGLKINKKKMLGIYET---PVTYQLAKALVFSKVRSALGLERCRFLMSGAGFLGPDTSEFFLSLDIPVGEMYGLSESSGPHTAHSWENYRILSCGKIL-SGCKNMLYQQNSDGTGEVCLWGRHVFMGYLEREQDTMEVIDEQGWLHSGDLGHMDSQGFLFISGRVKEIIITASGENVSPGPIENMIKKKIPILSHAMLVGDKAKFLSILLTLKCEMDSITGEPLDKLNSEAIKFCQSVGSQATTVTEIVGQHDLRVYAAIQRGIDAANQEANSGAHRVQKWVILEKDFSIWGGE-----------------LAAPAPAGHRYWTTKRDGEVYLRQXXXXXXXXXXXXXXXXXATKYANYIALGSKRRHGWHLLTYIELYEECRRAAKAFLQLGLKRFHGVGIMGLNSEEWVIASIGAIMAG----------SPKVCQVIAESSEMNVFVVDDDRQLQKIMQIQGCLKHLKAMVQYKEK--IQTQLQNLYSWRAFLELADSISDDALNRVIDSQKPNQCCALIYRPVATGPPKAIMLSHDNITWTTWATAQSLSYKHPPEGQEVLVSYLPLSYMGAQIFDMWVTISVAGALYFAQPGALRRVPRAGGHSHQWGPAVGSSAQLGAEGRAGAEGLVEPPPCRVAQGSLVDTLREVKPTTFYGVPWIWERLLDNL--KTSQLACT-PFRRTVNKWAMGLGLETNRRRMLGQIYQPLCFRLARKLAFNHARRFLGLHRCLQLFNMGLGLPQATRDYFLSLDMPVFELYGLSESSGIHTLSRQQDFRLLSCGKPLPGTYTRVQKQDSGDIHIWGRNIFMGYLNDETTTREKMDYHGWMHTGDLGFLDTEEFLYVVGSVRDVITLSSGERINPNPIEERVKKYVPIVHYAVLVGQDAPYLCALLTLKVNTDT-GQPRDMLTSEAIAFCRQLRSPSTRLSDIIDDGDPVVMEFIGQGIVAANAEAASDSARIAKWAILETDFSVAGGELGATTKLKRAIVAKIY 1308 HSP 2 Score: 282.722 bits (722), Expect = 4.660e-74 Identity = 161/423 (38.06%), Postives = 242/423 (57.21%), Query Frame = 0
Query: 648 DGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVV---IEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVE----KHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIK--------NLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIF 1051
DG V++R + P+++P + + + G+ A+ K +W ++QYY R AA+A IKLGL+ FH V I FN EW+I++LG I AGG + G+Y +NSA +Y++ ++ IL+VEN+ LQK+ I ++ LK ++ + + D L W D +ELG + QL Q + ANQC ++YTSGTTGNPKGVM+SHD +T+TA V H K+ +VSYLPLSHIAA LD++V + +YFA ALKG+LV TL+EV+PT+F GVPR++EK QE I+E ++ + +K + + + N KK + L +++ K++ +LGL++C+ G L P T++FFLSLDI + YGLSE G + + Y+ +CG I
Sbjct: 26 DGEVLLRLSRHGPGHETPVTIPELFRESVNRFGTYPALVSKSTENWEILNFNQYYEACRKAARALIKLGLERFHGVGILGFNSAEWLIASLGTILAGGLSVGIYATNSAEACQYVITRAKVNILLVENDLQLQKILSIPQSSIETLKAIIQYKLPMNVHNKDNL-YSWDDFMELGNSIPNSQLDQIIAGQKANQCAVLMYTSGTTGNPKGVMLSHDNITWTAGAVSRDCGFSHAREKQEVLVSYLPLSHIAAQMLDVWVHMKIGAVMYFAQPDALKGTLVDTLQEVKPTIFLGVPRIWEKMQERIKENGTRDSQLKKKVFSWARAIGLKIN---KKKMLGIYETPVTYQLAKALVFSKVRSALGLERCRFLMSGAGFLGPDTSEFFLSLDIPVGEMYGLSESSGPHTAHSWENYRILSCGKIL 444 HSP 3 Score: 102.449 bits (254), Expect = 4.402e-18 Identity = 52/131 (39.69%), Postives = 84/131 (64.12%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMG 1443
K++ ITA EN++P IE+ IK +P+LS+ + +GD KF+S+ LT K D G+P +L++ +C S+ +A+TVT I+ L V +AIQ+GID AN+ A S A +V+KW ++ ++ S+ GE+
Sbjct: 511 KEIIITASGENVSPGPIENMIKKKIPILSHAMLVGDKAKFLSILLTLKCEMDSITGEPLDKLNSEAIKFCQSVGSQATTVTEIVGQHDLRVYAAIQRGIDAANQEANSGAHRVQKWVILEKDFSIWGGELA 641 HSP 4 Score: 79.7221 bits (195), Expect = 3.759e-11 Identity = 45/145 (31.03%), Postives = 78/145 (53.79%), Query Frame = 0
Query: 1301 FRYVVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
F YVV R + + E I P IE+++K ++P++ V +G ++ LT KVN+D GQP L++ +C L ++ +++II D V+ I +GI AN A S++A++ KW+++ + S+ GE+G T
Sbjct: 1158 FLYVVGSVR-----DVITLSSGERINPNPIEERVKKYVPIVHYAVLVGQDAPYLCALLTLKVNTD-TGQPRDMLTSEAIAFCRQLRSPSTRLSDIIDDGDPVVMEFIGQGIVAANAEAASDSARIAKWAILETDFSVAGGELGAT 1296
BLAST of EMLSAG00000003336 vs. nr
Match: gi|831239309|ref|XP_012665595.1| (PREDICTED: LOW QUALITY PROTEIN: long-chain-fatty-acid--CoA ligase ACSBG2 [Otolemur garnettii]) HSP 1 Score: 532.332 bits (1370), Expect = 1.071e-162 Identity = 354/1060 (33.40%), Postives = 550/1060 (51.89%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGN-WVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKN-VTTLKKIV----VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEV-YFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYEN-----GDQK--VIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITLVNNVGFISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW----TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN--------LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQGR 1057
++P + + G+ AL + +NGN W N+ QYY A++ +KLGL+ F+ V I F +WF++ LG+I AGGL G+Y TNS +Y++ ++ IL+VEN Q ++ I I ++ + TLK IV I+E G G + W D +ELG +L + NQC +I TSGT G PKG MLSHD I +M A+ K + +VVSYLPLSHIAA +DI+ + G V YFA AL G+L+ TL+ V+P +F GVP+VWEK E I+E++ K +M ++ ++ N G Q+ + + + + L+F K++ +LGLD C IS AP+++ T +F SLDIP+ E +GL+E G +F+ +C I+ + + + IG EIC+ R +E T E F +EG+ H TG +K++L+T+GGENV P +E +K +P++ A F+S+ LT++ + + G+P +L+ V NLG +TV EIL + P AIQ+ N+++ISN K++KW IL ++ S GE+G Y TT G V +R+ ++ D P++V + K + IA+ K +W TY +YY E R AAKAF+KLGL+ FH V I N EW+I+++GAI AGGF+ G+ +++ + + N+ I VV+N+ LQK+ I ++KHL +V +E W+ ++L + + +L Q + + NQCC+++++ TG PK VM+SHD +T+T + V+ + + +V+YLPL+++ A D++V I G LYFA +KGSL+ L+EVRPT F+GVP ++++ + + K S+L S K A T K+ + L + + + GL+ CQ+F G L T DFFLSL+I I YGL+EC GI S + ++ + G P R
Sbjct: 1 MTIPELFXDSVSWFGTHPAL-VSKNGNKWEILNFSQYYEACWKAARSLLKLGLECFHGVGILGFKSVQWFVAALGSIIAGGLCVGIYATNSVEACQYVIGQAKVNILLVENDQQIQKIILIPRSRLETLKAIVRYRLPIKESGDNRG--FVSWDDFMELGSSIPDTQLERIIESQKANQCALIICTSGTIGPPKGAMLSHDNIMWMAGAVSKDFGLLEKRELVVSYLPLSHIAAQMMDIWXKI---GAVTYFAQPDALWGTLLSTLQNVKPALFMGVPQVWEKVYETIREKNAKYLKKKM----FLWAKTIGFWTNTKRMVGKQETNMSYYVANSLVFSKIRSSLGLDHCHISISVAAPITKETAEF-XSLDIPIGEVHGLTESSGPHAISTYYNFRLLSCSKILTGCKNMPFEQNKEGIG-EICMSDRQSSWTTWRSEVETLEVFGEEGWLHSADLGHMDNQGFLYITGHIKEILITAGGENVXPIHMETLVKE-IPIIXKA-------NFLSMLLTVECEKDPVSGQPLDQLSMEVINFCWNLGSKASTVTEILKMREPMFYAAIQQ--XTINQQSISNVQKIQKWVILGKDFSTSGGELG-----------------------SSYWTTHRHGEVRLRQDENSFRDKAPLTVHDMIMGTATKYANYIALGSKYKDNWHLLTYIEYYEECRRAAKAFLKLGLQRFHGVGIMGLNSEEWVIASIGAIMAGGFSVGILTNSTPKSCQMIAENAEMDIFVVDNDRQLQKILQIQGSLKHLKAIVQYKEVIQTEQTNLYSWQSFLDLAEGVSDYRLDQVIDSQKPNQCCTLVFSLSATGPPKAVMLSHDNITWTTEATVQSLCYKCPPEGQEVLVNYLPLAYMGAQIFDMWVTIEVAGALYFAQPDGVKGSLMDILREVRPTTFYGVPWIWDRMLDNL--KASQL-DSTTLRRKFDKWAMMLGLRTNQKRMFGHIDLPLCFGLAKSLTFNPARKLFGLEHCQQFFNMGPGLPRATLDFFLSLNIPIFEFYGLTECTGIHSISSHKAFRLLSSGKGLPRAHTR 1012 HSP 2 Score: 348.206 bits (892), Expect = 2.792e-96 Identity = 211/625 (33.76%), Postives = 324/625 (51.84%), Query Frame = 0
Query: 20 LASSYTTTKPDKGVHIRTESSEF---LSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTT---AKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKE---QSKNQKKSQMKVAQM--FKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTGRL---------------KDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIG 616
L SSY TT V +R + + F +V +M+ + AL NW Y +YY + + AKAF+KLGL F+ V I N EW I+++GAI AGG S G+ ++ + + + N+ I VV+N + L+ I I ++ LK IV +E NL W+ L+L RL+ + NQCC+L+++ TG PK VMLSHD IT+ T+A V+ + + ++V+YLPL+++ A D++V + + G +YFA +KGSL+D L+EVRPT F+GVP +W++ + +K S ++ K A M + + + D + + L F ++ GL+ C +F + G L T DFFLSL+IP+ E YGL+EC GI F+ + G +P H +V K ++ + G I I GR + MGYL N++ T + D +G+ HTG L KD++ S GE V P PIE+ +K Y+P+V + VVVG ++ LT+K + N E GEP + LT + L + T + +I+ + VM I + ID N A S + K+ KW IL + S+ GE+G
Sbjct: 580 LGSSYWTTHRHGEVRLRQDENSFRDKAPLTVHDMIMGTATKYANYIALGSKYKDNWHLLTYIEYYEECRRAAKAFLKLGLQRFHGVGIMGLNSEEWVIASIGAIMAGGFSVGILTNSTPKSCQMIAENAEMDIFVVDNDRQLQKILQIQGSLKHLKAIVQYKEVIQTEQTNLYSWQSFLDLAEGVSDYRLDQVIDSQKPNQCCTLVFSLSATGPPKAVMLSHDNITWTTEATVQSLCYKCPPEGQEVLVNYLPLAYMGAQIFDMWVTIEVAGALYFAQPDGVKGSLMDILREVRPTTFYGVPWIWDRMLDNLKASQLDSTTLRRKFDKWAMMLGLRTNQKRMFGHIDLPLCFGLAKSLTFNPARKLFGLEHCQQFFNMGPGLPRATLDFFLSLNIPIFEFYGLTECTGIHSISSHKAFRLLSSGKGLPRAHTRVHKEDEEGV-GNISIWGRNIFMGYLNNKEETEKRMDAQGWMHTGDLGFLDVNKFLYITGNVKDIITLSSGEKVNPKPIEDQVKMYIPIVRHVVVVGQDAPYLCALLTLKCQVNMETGEPRNTLTSEAVAFCRQLKSHSTRLTDIINGQDHIVMGFISEVIDAINAEAQSESTKIVKWTILNTDFSIAGGELG 1203 HSP 3 Score: 85.1149 bits (209), Expect = 8.034e-13 Identity = 50/141 (35.46%), Postives = 81/141 (57.45%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPT 1190
F G KE+LITAGGEN+ PI +E +K +P++ F+S+ LT + D +GQP +LS V +C +LG +ASTVT I++ + P +AIQ+ N+ +SN +++KW ++ ++ S GE+G +
Sbjct: 453 FLYITGHIKEILITAGGENVXPIHMETLVKE-IPIIXKA-------NFLSMLLTVECEKDPVSGQPLDQLSMEVINFCWNLGSKASTVTEILKMREPMFYAAIQQ--XTINQQSISNVQKIQKWVILGKDFSTSGGELGSS 583 HSP 4 Score: 75.8702 bits (185), Expect = 4.827e-10 Identity = 42/140 (30.00%), Postives = 75/140 (53.57%), Query Frame = 0
Query: 1051 FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMG 1188
F G K+++ + GE + P IED +K ++P++ +VV +G ++ LT K VN +T G+P L++ +C L ++ +T+II Q VM I + ID N S + ++ KW+++ + S+ GE+G
Sbjct: 1065 FLYITGNVKDIITLSSGEKVNPKPIEDQVKMYIPIVRHVVVVGQDAPYLCALLTLKCQVNMET-GEPRNTLTSEAVAFCRQLKSHSTRLTDIINGQDHIVMGFISEVIDAINAEAQSESTKIVKWTILNTDFSIAGGELG 1203 HSP 5 Score: 72.4034 bits (176), Expect = 5.612e-9 Identity = 44/133 (33.08%), Postives = 77/133 (57.89%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K++ ITAG EN+ PI +E +K +P++ F+S+ LT + D +GQP +LS V ++C +L +ASTVT I++ + +AIQ+ N+ ++SN K++KW ++ ++ S GE+G +
Sbjct: 461 KEILITAGGENVXPIHMETLVKE-IPIIXKA-------NFLSMLLTVECEKDPVSGQPLDQLSMEVINFCWNLGSKASTVTEILKMREPMFYAAIQQ--XTINQQSISNVQKIQKWVILGKDFSTSGGELGSS 583 HSP 6 Score: 68.1662 bits (165), Expect = 1.065e-7 Identity = 40/140 (28.57%), Postives = 73/140 (52.14%), Query Frame = 0
Query: 1314 KQLFITAGV---------ENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMG 1443
K L+IT V E + P IED++K ++P++ +VV +G ++ LT K + + G+P L++ +C L ++ +T+II + V+ I + ID N A S + K+ KW+++ + S+ GE+G
Sbjct: 1064 KFLYITGNVKDIITLSSGEKVNPKPIEDQVKMYIPIVRHVVVVGQDAPYLCALLTLKCQVNMETGEPRNTLTSEAVAFCRQLKSHSTRLTDIINGQDHIVMGFISEVIDAINAEAQSESTKIVKWTILNTDFSIAGGELG 1203
BLAST of EMLSAG00000003336 vs. nr
Match: gi|762113516|ref|XP_011439519.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X1 [Crassostrea gigas] >gi|762113518|ref|XP_011439520.1| PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X1 [Crassostrea gigas] >gi|762113520|ref|XP_011439521.1| PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X1 [Crassostrea gigas]) HSP 1 Score: 488.804 bits (1257), Expect = 3.766e-152 Identity = 273/630 (43.33%), Postives = 371/630 (58.89%), Query Frame = 0
Query: 19 ALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIH-------KIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECG---IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A A+ Y TK D+ V +R S +VP+M + A+A+ R G W++W Y QY+SD + AKA IKLGL+P + V I FN PEWFI+N GAIFAGG + G+Y TN++ +Y+ NS+C ++VVEN Q L+ I + ++ LK +V + +N+ WK+ +EL + D L RLS ++ N+ C LIYTSGTTGNPKGVMLSHD + + V+ A + +S+LPLSHIA S DIY A+ VYFA AL+GSL+ TLKEVRPT+F GVPRVWEK EGIK +S+ + K+ K Y ++ H KI + ++ K K LG DRC F+SG AP+ + T +FF L IP+ E YG+SEC CS+ + F+T + GT IP K+ D++ GE+ + GR V MGYL EQ T E FD+E Y + TGR K+L++T+GGEN+AP PIE+ +K LP VSN +++GD KF+S+ +T+KV N D EPT LT K G T V +IL HK V+KAIQKGID+AN+RAIS A K++KW ILPR+ S+ GE+G T+
Sbjct: 78 ASATEYVVTKRDQAVKLRMSQKGPGSEKPLTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVVQYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLCWTAGKAVENAALLYASEVQLSFLPLSHIAGSLTDIYGAMACAATVYFAQPDALRGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG---YRTTIAEMNHEPVPGAFKIAN-IMLNKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDR--FRTSSVGTEIPGVQTKLA-DKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 700 HSP 2 Score: 410.223 bits (1053), Expect = 5.289e-123 Identity = 242/642 (37.69%), Postives = 354/642 (55.14%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISI-VSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN---LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQ-----------------GR-----------------KKELLITAG-------------------------GENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
+ + A EY T D +V +R + +P++VPS + + A+A+KR G W++WTY QY+ + + AAKA IKLGL+P H V I FN PEW I+N GAIFAGGF G+Y +N+A +Y+ NS+C ++VVENN L+K+ + +++ L VV + G +A+K + W++ +EL +D L+ RL +A N+ C +IYTSGTTGNPKGVM+SHD L +TA VE + +S+LPLSHIA + DIY + C +YFA AL+GSL+ TLKEVRPTVF GVPRV+EK EGI+ K + +K I K Y TT N + + + N ML K K LG D+C+ F G AP+M T +FF L I + YG+SEC G + + ++ + G+ P Q GR +EL + +G GENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ +W + G A+ V++I+ + V+ AIQKGID ANE +S A +++KWS++PR+ S+ GE+GPT+KL+R K++
Sbjct: 75 DDLASATEYVVTKRDQAVKLRMSQKGPGSEKPLTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVV--QYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLCWTAGKAVENAALLYASEVQLSFLPLSHIAGSLTDIYGAMACAATVYFAQPDALRGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG--YRTTIAEMNHEPVPGAFKIANIML-NKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDRFRTSSVGTEIPGVQTKLADKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTMKLRRPIVHKMY 711 HSP 3 Score: 130.183 bits (326), Expect = 4.733e-27 Identity = 65/138 (47.10%), Postives = 97/138 (70.29%), Query Frame = 0
Query: 1310 GRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
GRK K+L ITAG ENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ + DW + A+ V++I+ K V+ AIQKGIDKAN+ A+S A K++KWS++PR+ S+ GE+GPT+
Sbjct: 564 GRK-KELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 700
BLAST of EMLSAG00000003336 vs. nr
Match: gi|762113522|ref|XP_011439522.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X2 [Crassostrea gigas]) HSP 1 Score: 488.419 bits (1256), Expect = 5.042e-152 Identity = 272/630 (43.17%), Postives = 371/630 (58.89%), Query Frame = 0
Query: 19 ALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIH-------KIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECG---IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A A+ Y TK D+ V +R S +VP+M + A+A+ R G W++W Y QY+SD + AKA IKLGL+P + V I FN PEWFI+N GAIFAGG + G+Y TN++ +Y+ NS+C ++VVEN Q L+ I + ++ LK +V + +N+ WK+ +EL + D L RLS ++ N+ C LIYTSGTTGNPKGVMLSHD +T+ ++V+YLPLSHIA +DIY+++ VYFA ALKGSL+ TLKEVRPT+F GVPRVWEK EGIK +S+ + K+ K Y ++ H KI + ++ K K LG DRC F+SG AP+ + T +FF L IP+ E YG+SEC CS+ + F+T + GT IP K+ D++ GE+ + GR V MGYL EQ T E FD+E Y + TGR K+L++T+GGEN+AP PIE+ +K LP VSN +++GD KF+S+ +T+KV N D EPT LT K G T V +IL HK V+KAIQKGID+AN+RAIS A K++KW ILPR+ S+ GE+G T+
Sbjct: 78 ASATEYVVTKRDQAVKLRMSQKGPGSEKPLTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVVQYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLTWCAGTFSHSFKIQRDSEVMVTYLPLSHIAGQIVDIYISMKYGAVVYFAKPDALKGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG---YRTTIAEMNHEPVPGAFKIAN-IMLNKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDR--FRTSSVGTEIPGVQTKLA-DKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 700 HSP 2 Score: 405.986 bits (1042), Expect = 2.316e-121 Identity = 242/642 (37.69%), Postives = 354/642 (55.14%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIR---RKKDDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISI-VSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN---LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQ-----------------GRK-----------------KELLITAG-------------------------GENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
+ + A EY T D +V +R + +P++VPS + + A+A+KR G W++WTY QY+ + + AAKA IKLGL+P H V I FN PEW I+N GAIFAGGF G+Y +N+A +Y+ NS+C ++VVENN L+K+ + +++ L VV + G +A+K + W++ +EL +D L+ RL +A N+ C +IYTSGTTGNPKGVM+SHD LT+ A T + + V+YLPLSHIA +DIY+ + +YFA ALKGSL+ TLKEVRPTVF GVPRV+EK EGI+ K + +K I K Y TT N + + + N ML K K LG D+C+ F G AP+M T +FF L I + YG+SEC G + + ++ + G+ P Q GR +EL + +G GENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ +W + G A+ V++I+ + V+ AIQKGID ANE +S A +++KWS++PR+ S+ GE+GPT+KL+R K++
Sbjct: 75 DDLASATEYVVTKRDQAVKLRMSQKGPGSEKPLTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVV--QYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLTWCAGTFSHSFKIQRDSEVMVTYLPLSHIAGQIVDIYISMKYGAVVYFAKPDALKGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG--YRTTIAEMNHEPVPGAFKIANIML-NKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDRFRTSSVGTEIPGVQTKLADKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTMKLRRPIVHKMY 711 HSP 3 Score: 129.798 bits (325), Expect = 5.738e-27 Identity = 65/138 (47.10%), Postives = 97/138 (70.29%), Query Frame = 0
Query: 1310 GRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
GRK K+L ITAG ENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ + DW + A+ V++I+ K V+ AIQKGIDKAN+ A+S A K++KWS++PR+ S+ GE+GPT+
Sbjct: 564 GRK-KELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 700
BLAST of EMLSAG00000003336 vs. nr
Match: gi|1005421894|ref|XP_015764088.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X1 [Acropora digitifera]) HSP 1 Score: 486.493 bits (1251), Expect = 1.713e-151 Identity = 268/628 (42.68%), Postives = 379/628 (60.35%), Query Frame = 0
Query: 19 ALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIV--VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK-HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMH---YYENGDQKVIH-KIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFK-TGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A A S TTK D GV +R SS S +V ++ ++ AL++ RNG WVKWNY +YY ++ AK+FIKLGL+P++ V I FN PEW IS+LGAIFAGGL+ G+Y TNS Y+ N ILVVEN Q L+ I + + LK +V + E +G RP N+ W + + L + D L R+ ++ N+CC+LIYTSGTTGNPKGVMLSHD +T+ AIV+ H VS+LPLSHIAA LDI++ + L G ++FA ALKG++V+TL+EVRPT+F VPRV+EK E + EQ++ + + + ++ ENG++ + +L+ K++ ALGLDRC G AP++ T +F S++IP+ E +G+SEC +V +G+CG + KV + D+ E+C+KGR V MGYL N T EA D++G+ H TGR+K++++T+GGENVAP PIE+ IK LP+VSNA+V+GD +KF+S LT+KV N + EPT L+ + ++G N TV EIL K +MKAIQ+GID AN ++IS A +V+KW IL ++ S+ GE+G TL
Sbjct: 59 APAESTWTTKADDGVKLRMGSSGATSRVPITVVQKLERAVRVAPNRVALSVKRNGEWVKWNYQEYYGAVRCAAKSFIKLGLEPYHGVGILGFNAPEWIISDLGAIFAGGLAVGIYATNSPEACHYVAENCDANILVVENDQQLKKILQVWDRLPHLKAVVQYIGEIEGERP-TNVYTWNEFMALSKDVTDDTLQARIDNLVPNKCCTLIYTSGTTGNPKGVMLSHDNVTWAADAIVRDIHAGEKGPESSVSFLPLSHIAAQQLDIFLPITLAGSLWFAQPDALKGTIVETLREVRPTLFCAVPRVYEKIMEKMNEQARQMSGLGRMIGKWAQGVALQGNINKENGEEVPYGWTVATRLVLNKIRVALGLDRCRLCFVGAAPVTMETLRYFQSINIPLNEIFGMSECSGPQTVSVPGCTISGSCGVAVDGCEMKVA-NEDEQGNAELCLKGRHVFMGYLNNVDKTEEALDEDGWLHTGDVAKIDKNGFLYITGRIKEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTL 684 HSP 2 Score: 305.449 bits (781), Expect = 1.911e-85 Identity = 162/398 (40.70%), Postives = 240/398 (60.30%), Query Frame = 0
Query: 662 PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV--VIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEK--HGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK------FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCG 1048
P++V ++ +++A+++KRNG+W++W Y +YY +R AAK+FIKLGL+P+H V I FN PEW+IS+LGAIFAGG A G+Y +NS Y+ N ILVVEN+ L+K+ + + + HL VV + E +G + W + + L K +D L+ R+ N+ N+CC++IYTSGTTGNPKGVM+SHD +T+ A +V G S VS+LPLSHIAA LDI++ I G L+FA ALKG++V TL+EVRPT+F VPRVYEK E + E+ ++ + I + A N NK+ + + +++ KI+ +LGLD+C+ G AP+ T +F S++I + +G+SEC G + P G+CG
Sbjct: 87 PITVVQKLERAVRVAPNRVALSVKRNGEWVKWNYQEYYGAVRCAAKSFIKLGLEPYHGVGILGFNAPEWIISDLGAIFAGGLAVGIYATNSPEACHYVAENCDANILVVENDQQLKKILQVWDRLPHLKAVVQYIGEIEGERPTNV-YTWNEFMALSKDVTDDTLQARIDNLVPNKCCTLIYTSGTTGNPKGVMLSHDNVTWAADAIVRDIHAGEKGPESSVSFLPLSHIAAQQLDIFLPITLAGSLWFAQPDALKGTIVETLREVRPTLFCAVPRVYEKIMEKMNEQARQMSGLGRMIGKWAQGVALQGNI--NKENGEEVPYGWTVATRLVLNKIRVALGLDRCRLCFVGAAPVTMETLRYFQSINIPLNEIFGMSECSGPQTVSVPGCTISGSCG 481 HSP 3 Score: 141.739 bits (356), Expect = 1.050e-30 Identity = 70/141 (49.65%), Postives = 99/141 (70.21%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
GR KE++ITAGGEN+API IED IK LP++SN + IGD KKF+S LT KV + N +P+ LS + ++C S+G A TV+ I+ + +M AIQ+GID AN +S A RV+KW+++ ++ S+ GE+GPTLKL+R
Sbjct: 548 GRIKEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTLKLRR 688 HSP 4 Score: 124.79 bits (312), Expect = 2.035e-25 Identity = 62/134 (46.27%), Postives = 92/134 (68.66%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+API IED+IK LP++SN + IGD KKF+S LT KV + N +P+ LS D+C S+ A TV+ I+ K ++ AIQ+GID+AN ++S A +V+KW+++ ++ S+ GE+GPTL
Sbjct: 551 KEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTL 684
BLAST of EMLSAG00000003336 vs. nr
Match: gi|1007783546|ref|XP_015828743.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X2 [Nothobranchius furzeri]) HSP 1 Score: 484.182 bits (1245), Expect = 3.101e-150 Identity = 257/637 (40.35%), Postives = 383/637 (60.13%), Query Frame = 0
Query: 6 SNSMTDKMFPNKQALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQKVI--HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
++++ + P K+ +SS DK V +R E+S S ++ M G K AL +G WV + +YY + + AK+F+KLGL+ F+ V I FN PEWFIS++G IFAGGL+ G+Y TNS +Y+ N +LVVEN + L I + + LK I+ + + + L W + ++LG ++LN + + N+CCSLIYTSGTTGNPKGVMLSHD I++ + + K + + ++VSYLPLSH+AA +DI+ + L G +YFAD ALKGSLV+TLKEVRPT F GVPRVWEK QE +K + ++A K + Y NG+ V + + L+F++V+ LGLDRC +G AP+++ T ++F+SL+IPV+E YG+SE +V + ++ G+CG ++P K + D+D GEIC GR V MGYL T EA D++G+ H TGR+K+L++T+GGEN+ P PIE+A+K +P++SNA+++GD KF+S+ LT+K + +++GEPT +L+ V + G T V E+L +K P + KAIQ+G++ N R+ SNA KV+KW +L R+ S+ GE+G TL
Sbjct: 81 TSNLATTLAPAKELWSSSR-----DKAVRLRMENSGPGSETPMTIHQMFLETVEWCGDKPALVSKTDGRWVTLTWREYYEECRTAAKSFLKLGLERFHGVGILGFNSPEWFISDVGCIFAGGLATGIYTTNSPEACQYVAANCEADVLVVENQKQLDKILKVKGQLPHLKAIIQYKGELQQKAPFLYTWAEFMKLGEDVSNEQLNAVIDSLRSNECCSLIYTSGTTGNPKGVMLSHDNISWTARTVFKMINENYGEEVLVSYLPLSHVAAQMIDIWGCMSLGGTIYFADPDALKGSLVNTLKEVRPTGFLGVPRVWEKMQEKMKAAGSKASPLKKRLADWAKSVGLQYNYSVMNGENAVPWGFMLSNNLVFKRVRAGLGLDRCTFCCTGAAPITKDTLEYFMSLNIPVKELYGMSESSGPHTVSINQYRIGSCGKVMPGCKTK-LDHPDEDGNGEICFWGRHVFMGYLNMPDKTVEALDEDGWLHSGDLGKHDKDDFLYITGRIKELIITAGGENIPPVPIEDAVKTEVPIISNAMLIGDKLKFLSMMLTLKCEMDDNGEPTDDLSPEVLDFCQQHGVKSTKVSEVLANKEPAIYKAIQEGMERVNARSTSNAQKVQKWIVLGRDFSVVGGELGPTL 711 HSP 2 Score: 380.948 bits (977), Expect = 5.070e-112 Identity = 230/652 (35.28%), Postives = 349/652 (53.53%), Query Frame = 0
Query: 626 ISSSVLITEKIEDAKEYTTTLPDGSVIIRRKKD---DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVV----EKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN--LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECGIFSDFAPSEYKKGTCGSIFPSTQ-----------------GR--------------------------------KKELLITAGGENIAPILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
+S+S L T + AKE ++ D +V +R + PM++ G + A+ K +G W+ T+ +YY E R AAK+F+KLGL+ FH V I FN PEW IS++G IFAGG ATG+Y +NS +Y+ N +LVVEN L K+ + + HL +I+ G + K L W + ++LG+ +QL + ++ +N+CCS+IYTSGTTGNPKGVM+SHD +++TA+TV E +G + +VSYLPLSH+AA +DI+ + G +YFAD ALKGSLV TLKEVRPT F GVPRV+EK QE ++ SK +K + + K YN + G L N +++K+++ LGLD+C G AP+ T ++F+SL+I +K YG+SE + ++Y+ G+CG + P + GR K + L G I ++I +N PV +SN + IGD KF+S+ LT K D NG+P+ +LS V ++C G +++ V+ ++ ++ P + AIQ+G++ N SNA +V+KW ++ R+ S+ GE+GPTLKL+R K++
Sbjct: 79 LSTSNLAT-TLAPAKELWSSSRDKAVRLRMENSGPGSETPMTIHQMFLETVEWCGDKPALVSKTDGRWVTLTWREYYEECRTAAKSFLKLGLERFHGVGILGFNSPEWFISDVGCIFAGGLATGIYTTNSPEACQYVAANCEADVLVVENQKQLDKILKVKGQLPHL--KAIIQYKGELQQKAPFLYTWAEFMKLGEDVSNEQLNAVIDSLRSNECCSLIYTSGTTGNPKGVMLSHDNISWTARTVFKMINENYG---EEVLVSYLPLSHVAAQMIDIWGCMSLGGTIYFADPDALKGSLVNTLKEVRPTGFLGVPRVWEKMQEKMKAAGSKASPLKKRLADWAKSVGLQYNYSVMNGENAVPWGFMLSNNLVFKRVRAGLGLDRCTFCCTGAAPITKDTLEYFMSLNIPVKELYGMSESSGPHTVSINQYRIGSCGKVMPGCKTKLDHPDEDGNGEICFWGRHVFMGYLNMPDKTVEALDEDGWLHSGDLGKHDKDDFLYITG--RIKELIITAGGENIPPVPIEDAVKTEVPIISNAMLIGDKLKFLSMMLTLKCEMDDNGEPTDDLSPEVLDFCQQHGVKSTKVSEVLANKEPAIYKAIQEGMERVNARSTSNAQKVQKWIVLGRDFSVVGGELGPTLKLRRPVVVKMY 722 HSP 3 Score: 120.553 bits (301), Expect = 5.572e-24 Identity = 57/133 (42.86%), Postives = 89/133 (66.92%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENI P+ IED +K +P++SN + IGD KF+S+ LT K D NG+P+ +LS V D+C +++ V+ ++ +K + AIQ+G+++ N + SNA KV+KW ++ R+ S+ GE+GPTL
Sbjct: 579 KELIITAGGENIPPVPIEDAVKTEVPIISNAMLIGDKLKFLSMMLTLKCEMDDNGEPTDDLSPEVLDFCQQHGVKSTKVSEVLANKEPAIYKAIQEGMERVNARSTSNAQKVQKWIVLGRDFSVVGGELGPTL 711
BLAST of EMLSAG00000003336 vs. nr
Match: gi|1005421898|ref|XP_015764102.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X3 [Acropora digitifera]) HSP 1 Score: 482.641 bits (1241), Expect = 4.025e-150 Identity = 267/628 (42.52%), Postives = 378/628 (60.19%), Query Frame = 0
Query: 19 ALASSYTTTKPDKGVHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIV--VIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKK-HDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMH---YYENGDQKVIH-KIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFK-TGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNED-GEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
A A S TTK D GV +R SS S +V ++ ++ AL++ RNG WVKWNY +YY ++ AK+FIKLGL+P++ V I FN PEW IS+LGAIFAGGL+ G+Y TNS Y+ N ILVVEN Q L+ I + + LK +V + E +G RP N+ W + + L + D L R+ ++ N+CC+LIYTSGTTGNPKGVMLSHD +T+ AIV+ H VS+LPLSHIAA LDI++ + L G ++FA ALKG++V+TL+EVRPT+F VPRV+EK E + EQ++ + + + ++ ENG++ + +L+ K++ ALGLDR G AP++ T +F S++IP+ E +G+SEC +V +G+CG + KV + D+ E+C+KGR V MGYL N T EA D++G+ H TGR+K++++T+GGENVAP PIE+ IK LP+VSNA+V+GD +KF+S LT+KV N + EPT L+ + ++G N TV EIL K +MKAIQ+GID AN ++IS A +V+KW IL ++ S+ GE+G TL
Sbjct: 59 APAESTWTTKADDGVKLRMGSSGATSRVPITVVQKLERAVRVAPNRVALSVKRNGEWVKWNYQEYYGAVRCAAKSFIKLGLEPYHGVGILGFNAPEWIISDLGAIFAGGLAVGIYATNSPEACHYVAENCDANILVVENDQQLKKILQVWDRLPHLKAVVQYIGEIEGERP-TNVYTWNEFMALSKDVTDDTLQARIDNLVPNKCCTLIYTSGTTGNPKGVMLSHDNVTWAADAIVRDIHAGEKGPESSVSFLPLSHIAAQQLDIFLPITLAGSLWFAQPDALKGTIVETLREVRPTLFCAVPRVYEKIMEKMNEQARQMSGLGRMIGKWAQGVALQGNINKENGEEVPYGWTVATRLVLNKIRVALGLDRSRLCFVGAAPVTMETLRYFQSINIPLNEIFGMSECSGPQTVSVPGCTISGSCGVAVDGCEMKVA-NEDEQGNAELCLKGRHVFMGYLNNVDKTEEALDEDGWLHTGDVAKIDKNGFLYITGRIKEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTL 684 HSP 2 Score: 301.597 bits (771), Expect = 3.938e-84 Identity = 161/398 (40.45%), Postives = 239/398 (60.05%), Query Frame = 0
Query: 662 PMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVV--VIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEK--HGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK------FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCG 1048
P++V ++ +++A+++KRNG+W++W Y +YY +R AAK+FIKLGL+P+H V I FN PEW+IS+LGAIFAGG A G+Y +NS Y+ N ILVVEN+ L+K+ + + + HL VV + E +G + W + + L K +D L+ R+ N+ N+CC++IYTSGTTGNPKGVM+SHD +T+ A +V G S VS+LPLSHIAA LDI++ I G L+FA ALKG++V TL+EVRPT+F VPRVYEK E + E+ ++ + I + A N NK+ + + +++ KI+ +LGLD+ + G AP+ T +F S++I + +G+SEC G + P G+CG
Sbjct: 87 PITVVQKLERAVRVAPNRVALSVKRNGEWVKWNYQEYYGAVRCAAKSFIKLGLEPYHGVGILGFNAPEWIISDLGAIFAGGLAVGIYATNSPEACHYVAENCDANILVVENDQQLKKILQVWDRLPHLKAVVQYIGEIEGERPTNV-YTWNEFMALSKDVTDDTLQARIDNLVPNKCCTLIYTSGTTGNPKGVMLSHDNVTWAADAIVRDIHAGEKGPESSVSFLPLSHIAAQQLDIFLPITLAGSLWFAQPDALKGTIVETLREVRPTLFCAVPRVYEKIMEKMNEQARQMSGLGRMIGKWAQGVALQGNI--NKENGEEVPYGWTVATRLVLNKIRVALGLDRSRLCFVGAAPVTMETLRYFQSINIPLNEIFGMSECSGPQTVSVPGCTISGSCG 481 HSP 3 Score: 141.739 bits (356), Expect = 1.032e-30 Identity = 70/141 (49.65%), Postives = 99/141 (70.21%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTN-GQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR 1195
GR KE++ITAGGEN+API IED IK LP++SN + IGD KKF+S LT KV + N +P+ LS + ++C S+G A TV+ I+ + +M AIQ+GID AN +S A RV+KW+++ ++ S+ GE+GPTLKL+R
Sbjct: 548 GRIKEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTLKLRR 688 HSP 4 Score: 124.79 bits (312), Expect = 2.053e-25 Identity = 62/134 (46.27%), Postives = 92/134 (68.66%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKN-GQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+API IED+IK LP++SN + IGD KKF+S LT KV + N +P+ LS D+C S+ A TV+ I+ K ++ AIQ+GID+AN ++S A +V+KW+++ ++ S+ GE+GPTL
Sbjct: 551 KEIIITAGGENVAPIPIEDRIKKELPIVSNAMVIGDKKKFLSCLLTLKVIVNPNTAEPTDNLSGNAIDFCTSVGSNAKTVSEILSTKDENIMKAIQEGIDRANSQSISRAQRVQKWAILEKDFSIPGGELGPTL 684
BLAST of EMLSAG00000003336 vs. nr
Match: gi|762113524|ref|XP_011439523.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X3 [Crassostrea gigas]) HSP 1 Score: 479.559 bits (1233), Expect = 5.184e-150 Identity = 264/601 (43.93%), Postives = 359/601 (59.73%), Query Frame = 0
Query: 45 FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIH-------KIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECG---IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+VP+M + A+A+ R G W++W Y QY+SD + AKA IKLGL+P + V I FN PEWFI+N GAIFAGG + G+Y TN++ +Y+ NS+C ++VVEN Q L+ I + ++ LK +V + +N+ WK+ +EL + D L RLS ++ N+ C LIYTSGTTGNPKGVMLSHD + + V+ A + +S+LPLSHIA S DIY A+ VYFA AL+GSL+ TLKEVRPT+F GVPRVWEK EGIK +S+ + K+ K Y ++ H KI + ++ K K LG DRC F+SG AP+ + T +FF L IP+ E YG+SEC CS+ + F+T + GT IP K+ D++ GE+ + GR V MGYL EQ T E FD+E Y + TGR K+L++T+GGEN+AP PIE+ +K LP VSN +++GD KF+S+ +T+KV N D EPT LT K G T V +IL HK V+KAIQKGID+AN+RAIS A K++KW ILPR+ S+ GE+G T+
Sbjct: 12 LTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVVQYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLCWTAGKAVENAALLYASEVQLSFLPLSHIAGSLTDIYGAMACAATVYFAQPDALRGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG---YRTTIAEMNHEPVPGAFKIAN-IMLNKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDR--FRTSSVGTEIPGVQTKLA-DKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 605 HSP 2 Score: 404.831 bits (1039), Expect = 3.727e-122 Identity = 235/612 (38.40%), Postives = 342/612 (55.88%), Query Frame = 0
Query: 661 QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISI-VSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT---GNKKFIKN---LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQ-----------------GRK-----------------KELLITAG-------------------------GENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIH 1202
+P++VPS + + A+A+KR G W++WTY QY+ + + AAKA IKLGL+P H V I FN PEW I+N GAIFAGGF G+Y +N+A +Y+ NS+C ++VVENN L+K+ + +++ L VV + G +A+K + W++ +EL +D L+ RL +A N+ C +IYTSGTTGNPKGVM+SHD L +TA VE + +S+LPLSHIA + DIY + C +YFA AL+GSL+ TLKEVRPTVF GVPRV+EK EGI+ K + +K I K Y TT N + + + N ML K K LG D+C+ F G AP+M T +FF L I + YG+SEC G + + ++ + G+ P Q GR +EL + +G GENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ +W + G A+ V++I+ + V+ AIQKGID ANE +S A +++KWS++PR+ S+ GE+GPT+KL+R K++
Sbjct: 10 KPLTVPSMFYQTVQRVPNHPAIAVKRGGVWIQWTYQQYFSDTQKAAKALIKLGLEPLHGVGILGFNSPEWFIANNGAIFAGGFTVGIYSTNNADACKYVALNSQCNVIVVENNQQLKKILQVWDDLPDLKAVV--QYTGEVAEKRDNVYSWKEFMELSSQVSDDTLQHRLSLLAPNKACCLIYTSGTTGNPKGVMLSHDNLCWTAGKAVENAALLYASEVQLSFLPLSHIAGSLTDIYGAMACAATVYFAQPDALRGSLLGTLKEVRPTVFVGVPRVWEKMVEGIKHKSREFGFFKKKIGAWAKSVG--YRTTIAEMNHEPVPGAFKIANIML-NKAKGELGFDRCRIFMSGAAPIMKETIEFFYGLHIPLMEVYGMSECSGPHTVCSLDRFRTSSVGTEIPGVQTKLADKDEEGNGEVRMYGRHVFMGYLDEEQKTKETFDEELYLMSGDIGKKDKDGFLYITGRKKELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTMKLRRPIVHKMY 616 HSP 3 Score: 129.798 bits (325), Expect = 3.191e-27 Identity = 65/138 (47.10%), Postives = 97/138 (70.29%), Query Frame = 0
Query: 1310 GRKEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
GRK K+L ITAG ENIAP+ IED +K LP +SN + IGD +KF+S+ +T KV+ + + +P+ L+ + DW + A+ V++I+ K V+ AIQKGIDKAN+ A+S A K++KWS++PR+ S+ GE+GPT+
Sbjct: 469 GRK-KELIITAGGENIAPVPIEDTVKECLPCVSNCILIGDKRKFLSMLITLKVDVNMDTLEPTDNLTAVTVDWMKAQGSGATKVSDILDHKDAIVLKAIQKGIDKANERAISRAQKIQKWSILPRDFSIPGGELGPTM 605
BLAST of EMLSAG00000003336 vs. nr
Match: gi|676477130|ref|XP_009061014.1| (hypothetical protein LOTGIDRAFT_234625 [Lottia gigantea] >gi|556099647|gb|ESO88299.1| hypothetical protein LOTGIDRAFT_234625 [Lottia gigantea]) HSP 1 Score: 480.33 bits (1235), Expect = 5.609e-150 Identity = 256/622 (41.16%), Postives = 378/622 (60.77%), Query Frame = 0
Query: 23 SYTTTKPDKGVHIRTESS--EFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHD--IITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKD----ASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGE-PTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+++T + D V IR + ++P+ KN + TAL + N W+KW Y QYY K TAK+FIKLGL P Y+VCI FN PEWF ++LGAIFAGG+ G+Y TN++ +++ +S+ Q++VVEN L+ I + + LK IV + P +N+ W+D ++LG L +R+++++ N+CCSL+YTSGTTG PKGVMLSHD + T ++ + V++ K VS+LPLSHIAA LDIY+++ G +YFA ALKG+LV TLKE +PT FFGVPRVWEK E +KE +N ++K+A+ K + + ++ G I + L+F KVKQALGLD+C FISG AP+++ T ++F+ LDI + E YGLSEC G + K G+ G+ +P K + + D+D GEI I GR + MGYL + T E F K+GY TGR K++++T+GGEN+AP P+E+ +K LP +SN +++GD KF+S+ LT+K + + D P +L++ + K++G N TTV E + V +IQ+GI+ ANK+A+S AA ++KW +LP + S+ E+G TL
Sbjct: 11 TFSTVERDGAVQIRVGEGIGAIETQTIPDFFKNTVDKIPNNTALCVKINNAWIKWTYQQYYDSCKTTAKSFIKLGLQPHYSVCIMGFNSPEWFFADLGAIFAGGIGVGIYTTNNAEACQFIAADSKAQVIVVENTAYLKKILQVKDQLPDLKAIVQYSGEIETPQDNVYTWEDFMKLGEDLPESELEERMNNLAANKCCSLVYTSGTTGVPKGVMLSHDNLVWTAISASKVEEKRLIFGKNRAVSFLPLSHIAAQALDIYLSINNGGTIYFAQPDALKGTLVATLKEAKPTTFFGVPRVWEKMMEKMKEIGQNTTGLKLKIARWAKGVGYRGNQNRFQGGSVPYGWTIANFLVFSKVKQALGLDQCTNFISGAAPIAKDTIEYFMGLDIVIHEVYGLSECTGPHTVNYEEKRKIGSVGSCLPGLQMK-IDNPDEDGNGEILIYGRNICMGYLEQTEKTQETFTKDGYLRTGDIGQIDDNGFLSITGRSKEIIITAGGENIAPVPVEDNVKECLPCISNCMLLGDKRKFLSILLTVKTEVDPDTTVPLEKLSNPTLQWCKSIGSNATTVQE--FSDDETVQNSIQEGINAANKKAVSRAANIQKWKMLPTDFSIPGEELGPTL 629 HSP 2 Score: 388.652 bits (997), Expect = 6.830e-116 Identity = 235/638 (36.83%), Postives = 343/638 (53.76%), Query Frame = 0
Query: 640 KEYTTTLPDGSVIIRRKKD--DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYT---AKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT----GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQGR--------KKELLITAGGENIA-----------------------------------------PILIEDNIKNFLPV------------LSNVVAIGDLKKFISVFLTFKVNSDTNGQ-PSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSKIHFNI 1205
K ++T DG+V IR + ++ ++P F K+ K + A+ +K N W++WTY QYY + AK+FIKLGL+P ++V I FN PEW ++LGAIFAGG G+Y +N+A +++ +S+ Q++VVEN L+K+ + + + L +V + W D ++LG+ E +L++R++N+AAN+CCS++YTSGTTG PKGVM+SHD L +T A V EK K VS+LPLSHIAA ALDIY+ I G +YFA ALKG+LV TLKE +PT FFGVPRV+EK E ++E + I K + N G+ + + N +++ K+K +LGLDQC F G AP+ T ++F+ LDIVI YGLSEC G + + K G+ GS P Q + E+LI G NI I+I +N PV +SN + +GD +KF+S+ LT K D + P +LSN +WC S+G A+TV D+ V ++IQ+GI+ AN+ VS AA ++KW ++P + S+ E+GPTLKLKR +K + +I
Sbjct: 10 KTFSTVERDGAVQIRVGEGIGAIETQTIPDFFKNTVDKIPNNTALCVKINNAWIKWTYQQYYDSCKTTAKSFIKLGLQPHYSVCIMGFNSPEWFFADLGAIFAGGIGVGIYTTNNAEACQFIAADSKAQVIVVENTAYLKKILQVKDQLPDLKAIVQYSGEIETPQDNVYTWEDFMKLGEDLPESELEERMNNLAANKCCSLVYTSGTTGVPKGVMLSHDNLVWTAISASKVEEKRLIFGKNRAVSFLPLSHIAAQALDIYLSINNGGTIYFAQPDALKGTLVATLKEAKPTTFFGVPRVWEKMMEKMKEIGQNTTGLKLKIARWAKGVGYRGNQNRFQGGSVPYGWTIANFLVFSKVKQALGLDQCTNFISGAAPIAKDTIEYFMGLDIVIHEVYGLSECTGPHTVNYEEKRKIGSVGSCLPGLQMKIDNPDEDGNGEILIY--GRNICMGYLEQTEKTQETFTKDGYLRTGDIGQIDDNGFLSITGRSKEIIITAGGENIAPVPVEDNVKECLPCISNCMLLGDKRKFLSILLTVKTEVDPDTTVPLEKLSNPTLQWCKSIGSNATTVQEFSDDE--TVQNSIQEGINAANKKAVSRAANIQKWKMLPTDFSIPGEELGPTLKLKRQVVTKKYQSI 643 HSP 3 Score: 115.161 bits (287), Expect = 1.884e-22 Identity = 58/136 (42.65%), Postives = 88/136 (64.71%), Query Frame = 0
Query: 1312 KEKQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQ-PSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
+ K++ ITAG ENIAP+ +ED +K LP +SN + +GD +KF+S+ LT K D + P +LSN WC S+ A+TV D+ ++ ++IQ+GI+ ANK AVS AA ++KW ++P + S+ E+GPTL
Sbjct: 496 RSKEIIITAGGENIAPVPVEDNVKECLPCISNCMLLGDKRKFLSILLTVKTEVDPDTTVPLEKLSNPTLQWCKSIGSNATTVQEFSDDETVQ--NSIQEGINAANKKAVSRAANIQKWKMLPTDFSIPGEELGPTL 629
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold638_size121162-snap-gene-0.27 (protein:Tk11614 transcript:maker-scaffold638_size121162-snap-gene-0.27-mRNA-1 annotation:"long-chain-fatty-acid-- ligase acsbg2 isoform x1") HSP 1 Score: 523.472 bits (1347), Expect = 3.715e-170 Identity = 282/624 (45.19%), Postives = 394/624 (63.14%), Query Frame = 0
Query: 18 QALASSYTTTKPDKG-VHIRTESSEFLS---FSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYY-ENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKT-GTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGE-PTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
Q L S + TT G V IR F S SVP V+ G++ AL ++G W W+Y +Y++ I+ A+A IKLGL+P V I+ FN PEWF+S+LGAIFAGG S GLYPTNS NE++MN+S+ ILVVE+ + I + V +LK V+I+ G +++ W++ +E+G K D L DRL +++NQC +LIYTSGTTGNPKGVMLSHD I F +K + + ++SYLPLSHIAA LDI+ + K VYFADK ALKGSLV TL EVRPT FFGVPRVWEK E + ++ K K +Q K++ K+A ++Y+ N D V +G K++++KV+QALGLD+C F S AP+ T +FLSLDI + E YG+SE ++ ++ K G+ G + S ++V + SD+ GE+C+ GR +MMGYL E TS+ D EG+ H TGR+K+LL+T+GGENVAP PIE+ I+ +P+VSNAV++GD +KF+SV LT+K + + + PT +LT S + ++LG ++V ++L P +M AIQ GID N A+SNAAKV+KW ILP ++S+ GE+G T+
Sbjct: 8 QILPSDHLTTAERHGSVKIRYTKDGFGSRKAISVPTYVRKCAEEAGNRPALKFKKDGTWRTWSYTEYHAYIRCAARAMIKLGLEPCQAVSIFGFNSPEWFLSSLGAIFAGGFSCGLYPTNSVEMNEFIMNDSQTNILVVEDEAAMNKIWKATERVDSLK--VIIQYSGTPKKPSVLSWQEFMEIGAKASEDPLEDRLRQMAINQCAALIYTSGTTGNPKGVMLSHDNIVFTCDNALKTCSFRDFQERLISYLPLSHIAAMLLDIFAPIGSKSTVYFADKMALKGSLVATLIEVRPTQFFGVPRVWEKIMEKMLDKGKEIKGAQKKLSNTCKEAGLNYHLHNNDSFVYRTLGQKVVYKKVRQALGLDQCRAFYSAAAPIGVETLKYFLSLDIIIVELYGMSETAGPHTISTANQKRLGSVGKTMESCKSRVSEPSDEG-EGELCMWGRNIMMGYLGREDKTSQDIDPEGWMHSGDLAVFDEDGFVYITGRIKELLITAGGENVAPVPIEDLIRKEVPIVSNAVLIGDKQKFVSVFLTLKTELDPKTDAPTQQLTPSAQMWCESLGRRCSSVDDVLNGPDPIIMAAIQSGIDRVNAHAVSNAAKVQKWTILPNDVSIAGGELGPTM 628 HSP 2 Score: 454.136 bits (1167), Expect = 6.061e-144 Identity = 253/633 (39.97%), Postives = 365/633 (57.66%), Query Frame = 0
Query: 634 EKIEDAKEYTTTLPDGSVIIRRKKDDL---QPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTK-KISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFI-KNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSE-CGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGE---------------------NIAP-----------------ILIEDNIKNFL---------------------PVLSNVVAIGDLKKFISVFLTFKVNSDT-NGQPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEFSK 1200
++I + TT GSV IR KD + +SVP++V+ + G++ A+ K++G W W+Y++Y+ IR AA+A IKLGL+P VSI+ FN PEW +S+LGAIFAGGF+ GLYP+NS MNE++MN+S+ ILVVE+ + K+ E + L V+I+ G + W++ +E+G ED L+ RL +A NQC ++IYTSGTTGNPKGVM+SHD + +T ++ + + ++SYLPLSHIAA LDI+ I + +YFADK ALKGSLV TL EVRPT FFGVPRV+EK E + +K ++K +QK ++N K+A Y+ N F+ + LG +++YKK++ +LGLDQC+ F AP+ T +FLSLDI+I YG+SE G + ++ + G+ G S + R E GE +I P + I IK L P++SN V IGD +KF+SVFLT K D P+ +L+ S + WC SLG S+V +++ P +M+AIQ GID N VSNAA+V+KW+++P ++S+ GE+GPT+KLKR F+K
Sbjct: 7 DQILPSDHLTTAERHGSVKIRYTKDGFGSRKAISVPTYVRKCAEEAGNRPALKFKKDGTWRTWSYTEYHAYIRCAARAMIKLGLEPCQAVSIFGFNSPEWFLSSLGAIFAGGFSCGLYPTNSVEMNEFIMNDSQTNILVVEDEAAMNKIWKATERVDSL--KVIIQYSGTPKKPSVLSWQEFMEIGAKASEDPLEDRLRQMAINQCAALIYTSGTTGNPKGVMLSHDNIVFTCDNALKTCSFRDFQERLISYLPLSHIAAMLLDIFAPIGSKSTVYFADKMALKGSLVATLIEVRPTQFFGVPRVWEKIMEKMLDKGKEIKGAQKKLSNTCKEAGLNYHLHNNDSFVYRTLGQKVVYKKVRQALGLDQCRAFYSAAAPIGVETLKYFLSLDIIIVELYGMSETAGPHTISTANQKRLGSVGKTMESCKSRVSEPSDEGEGELCMWGRNIMMGYLGREDKTSQDIDPEGWMHSGDLAVFDEDGFVYITGRIKELLITAGGENVAPVPIEDLIRKEVPIVSNAVLIGDKQKFVSVFLTLKTELDPKTDAPTQQLTPSAQMWCESLGRRCSSVDDVLNGPDPIIMAAIQSGIDRVNAHAVSNAAKVQKWTILPNDVSIAGGELGPTMKLKRFAFNK 637 HSP 3 Score: 131.724 bits (330), Expect = 1.417e-31 Identity = 63/134 (47.01%), Postives = 93/134 (69.40%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSD-KNGQPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+AP+ IED I+ +P++SN V IGD +KF+SVFLT K D K P+ +L+ + WC SL S+V +++ +++AIQ GID+ N AVSNAAKV+KW+++P ++S+ GE+GPT+
Sbjct: 495 KELLITAGGENVAPVPIEDLIRKEVPIVSNAVLIGDKQKFVSVFLTLKTELDPKTDAPTQQLTPSAQMWCESLGRRCSSVDDVLNGPDPIIMAAIQSGIDRVNAHAVSNAAKVQKWTILPNDVSIAGGELGPTM 628
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold23_size669530-processed-gene-5.1 (protein:Tk11076 transcript:snap_masked-scaffold23_size669530-processed-gene-5.1-mRNA-1 annotation:"hypothetical protein LOTGIDRAFT_234625") HSP 1 Score: 466.077 bits (1198), Expect = 1.440e-148 Identity = 257/626 (41.05%), Postives = 372/626 (59.42%), Query Frame = 0
Query: 21 ASSYTTTKPDKGVHIRTESSE----FLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYEN------GDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGY---------------FHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
+ ++T GV +R + S +VP++++ +K AL++ R+G W+ W Y QY+ +++ AK FIKLGL+P V I FN PEWFIS+L A+FA G++ G+YPTNS +Y++N+S+ I+VVE+ + L + ++ LK I I+ G E ++ W DLL LG KE L+ RL I VNQCC L+YTSGTTG+PKGVMLSHD +T+ ++ + +VSYLPLSH+AA+ DI++ + +KG YFADK ALKG+L +TLKEV PT+F GVPRVWEK E + E K + ++ K + +N + +K+ DKL+FQKVK ALGL C F S APL+ ++F+SLD+ V E YG+SEC G S + G+ G + H+K+ D EI + GR +MMGYL +E T AFD EG+ F TGRLK++L+T+GGENVAP IEN +K +LP +SN +V+GD KF+S LT+KV+ ++D EP + L+ V + + +G T E + V +AIQ+GI+ AN+ AISNA +V+KW ++P++ S+ GE+G T+
Sbjct: 7 SDTFTAFDRHDGVKLRIDKSAHPDGMEPVTVPSLLQEVANEIPNKVALSVKRDGEWINWTYYQYHQEVRAVAKGFIKLGLEPRKAVGIIGFNSPEWFISDLAAVFANGIATGIYPTNSIEACKYIVNDSQMNIVVVEDQRQLDKFLAVKPDLAELKAI--IQYSGTPKAEGVLSWSDLLALGNKESDVELDQRLDDIYVNQCCHLVYTSGTTGSPKGVMLSHDNLTYTATTMIDGFGLRVNEETIVSYLPLSHVAANVTDIFLMIAIKGTTYFADKDALKGTLTNTLKEVGPTLFMGVPRVWEKIYEKMVELGKGNTGLKKQIGTWAKKQGFVHNQNVIKNQANSKSFQYKLADKLVFQKVKAALGLQNCDVFYSAAAPLAPNIIEYFMSLDMRVLEIYGMSECSGPQASNVLHHQRVGSIGKTLEGFHSKI-----DPEESEIRLLGRNIMMGYLGDETKTLAAFDAEGWLKSGDIGREDPDDFLFITGRLKEILITAGGENVAPILIENTLKKHLPAISNVMVIGDKRKFLSCLLTLKVEVDQDSMEPLNTLSKPVLEWCEEIGSKAKTAQEAIDDDV--VRRAIQEGINRANEEAISNAQRVQKWKLIPKDFSIPGGELGPTM 623 HSP 2 Score: 466.077 bits (1198), Expect = 1.648e-148 Identity = 256/612 (41.83%), Postives = 358/612 (58.50%), Query Frame = 0
Query: 658 DDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQK---VQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW-TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFI--TNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC----------------------GIFSDFAPSE------------------------------YKKGTCGSIFPS----TQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
D ++P++VPS ++ + +++A+++KR+G+W+ WTY QY++E+R AK FIKLGL+P V I FN PEW IS+L A+FA G ATG+YP+NS +Y++N+S+ I+VVE+ L K V+P L +K +I+ G + + W DL+ LG E + +L QRL +I NQCC ++YTSGTTG+PKGVM+SHD LTYTA T+++ G + +IVSYLPLSH+AAN DI+++I +G YFADK ALKG+L TLKEV PT+F GVPRV+EK E G++++I K Q F+ N++K N +K F L ++++++K+K +LGL C F APL P ++F+SLD+ + YG+SEC G S P E K G G P GR KE+LITAGGEN+APILIE+ +K LP +SNV+ IGD +KF+S LT KV D + +P LS V EWC +G +A T I D V AIQ+GI+ ANE +SNA RV+KW LIP++ S+ GE+GPT+KLKR+
Sbjct: 30 DGMEPVTVPSLLQEVANEIPNKVALSVKRDGEWINWTYYQYHQEVRAVAKGFIKLGLEPRKAVGIIGFNSPEWFISDLAAVFANGIATGIYPTNSIEACKYIVNDSQMNIVVVEDQRQLDKFLAVKPDLAELK-----AIIQYSGTPKAEGVLSWSDLLALGNKESDVELDQRLDDIYVNQCCHLVYTSGTTGSPKGVMLSHDNLTYTATTMIDGFGLRVNEETIVSYLPLSHVAANVTDIFLMIAIKGTTYFADKDALKGTLTNTLKEVGPTLFMGVPRVWEKIYEKMVELGKGNTGLKKQIGTWAKKQGFVHNQNVIK------NQANSKSFQYKLADKLVFQKVKAALGLQNCDVFYSAAAPLAPNIIEYFMSLDMRVLEIYGMSECSGPQASNVLHHQRVGSIGKTLEGFHSKIDPEESEIRLLGRNIMMGYLGDETKTLAAFDAEGWLKSGDIGREDPDDFLFITGRLKEILITAGGENVAPILIENTLKKHLPAISNVMVIGDKRKFLSCLLTLKVEVDQDSMEPLNTLSKPVLEWCEEIGSKAKTAQEAIDDDV--VRRAIQEGINRANEEAISNAQRVQKWKLIPKDFSIPGGELGPTMKLKRH 628 HSP 3 Score: 127.102 bits (318), Expect = 4.379e-30 Identity = 64/134 (47.76%), Postives = 91/134 (67.91%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K++ ITAG EN+APILIE+ +K LP +SNV+ IGD +KF+S LT KV D++ +P LS V +WC + +A T I D V AIQ+GI++AN+ A+SNA +V+KW LIP++ S+ GE+GPT+
Sbjct: 492 KEILITAGGENVAPILIENTLKKHLPAISNVMVIGDKRKFLSCLLTLKVEVDQDSMEPLNTLSKPVLEWCEEIGSKAKTAQEAIDDDV--VRRAIQEGINRANEEAISNAQRVQKWKLIPKDFSIPGGELGPTM 623
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold124_size330879-processed-gene-1.10 (protein:Tk01487 transcript:snap_masked-scaffold124_size330879-processed-gene-1.10-mRNA-1 annotation:"long-chain-fatty-acid-- ligase acsbg2") HSP 1 Score: 464.922 bits (1195), Expect = 6.965e-148 Identity = 259/636 (40.72%), Postives = 371/636 (58.33%), Query Frame = 0
Query: 11 DKMFPNKQALASSYTTTKPDKGVHIRTESS--EFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVI--------HKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDG-EPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
D++ P + + T +P+ V +R S SVP +++ + ALA+ R G+WV W+Y QYY+D + AK F+ LGL+PF++V I FN PEWFI+ AI A G SAG+Y TNS ++ + R ILVVE+ + L I I + LK V++ G E +I W L+ELG +E D L RLS I++NQCCSLIYTSGTTG+PKGVM+SHD T+ T I T + +SYLPLSH AA +D+++ + + VYFAD+ ALKGSLV+TLKEVRPT FFGVPR++EK QE + + K+ + VA K + + + I + + KL+F KV++ LGLD+C G AP++ T ++FLSLDIP+ ECYG+SE G + + G+ G + K+ + +D D GE+ ++GR +MMGYLFNE+ T EA D EG+ H TGR+K+L++T+GGENV P IE+ +K LP +SN +V+GD +KF+S LT+KV+ + D EP EL K +G T+ E + V KA+Q+G+D N++A SNA +++KW +L + S+ GE+G TL
Sbjct: 11 DQLLPTP---SQRWWTCQPEGSVALRDRESMSAVPPLSVPTLLRQAARDAPDQLALAVKREGHWVHWSYAQYYADSRTAAKGFVALGLEPFHSVGILGFNAPEWFIAQNAAIMAHGFSAGIYTTNSPEACLHIALDCRANILVVEDEKQLDKILQIRPQLPDLK--CVVQYLGTPHKEGVISWARLMELGAQESDDELERRLSAIAINQCCSLIYTSGTTGSPKGVMMSHDNFTW-TARIANSFLKVTTEDSFISYLPLSHSAAQMIDVWMPMAARASVYFADRNALKGSLVNTLKEVRPTFFFGVPRIYEKIQEKMVQIGKSSGAVKRLVADWAKRTGLQHNLRALEGQITPNQPGYSYPLAKKLVFSKVQENLGLDKCRIMGVGAAPMARETFEYFLSLDIPLLECYGMSETSGPQTGNRPGQHRLGSIGPSLDGCKTKIFE-ADPDGHGEVLMRGRNIMMGYLFNEEKTREAIDDEGWLHSGDIGKELAESYFKITGRIKELIITAGGENVPPVLIEDVLKKELPCLSNTMVIGDRKKFLSCLLTLKVEVDVDTLEPKPELAAGALDWCKEIGSKAKTIDEAANDEL--VRKALQEGVDRTNQQATSNAQRIQKWHLLNHDFSVPGGELGPTL 637 HSP 2 Score: 400.208 bits (1027), Expect = 7.815e-124 Identity = 234/639 (36.62%), Postives = 348/639 (54.46%), Query Frame = 0
Query: 639 AKEYTTTLPDGSVIIRRKKD--DLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKV---QPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKN------------LGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSEC-GIFSDFAPSEYKKGTCGSIFPSTQGRKKELL---------ITAGGEN-----------------------------------------IAPILIEDNIKNFLPVL------------SNVVAIGDLKKFISVFLTFKVNSDTNG-QPSTELSNSVKEWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
++ + T P+GSV +R ++ + P+SVP+ ++ Q+A+A+KR G W+ W+Y+QYY + R AAK F+ LGL+PFH+V I FN PEW I+ AI A GF+ G+Y +NS ++ + R ILVVE+ L K+ +P L ++K V++ G + I W L+ELG E +D+L++RL IA NQCCS+IYTSGTTG+PKGVM+SHD T+TA+ T + S +SYLPLSH AA +D+++ + R +YFAD++ALKGSLV TLKEVRPT FFGVPR+YEK I+EK+ ++ KS + ++ D A N + ++ L ++++ K++++LGLD+C+ G AP+ T ++FLSLDI + YG+SE G + P +++ GSI PS G K ++ + G N I ++I +N PVL SN + IGD KKF+S LT KV D + +P EL+ +WC +G +A T+ D+ V A+Q+G+D N+ SNA R++KW L+ + S+ GE+GPTLKLKR+
Sbjct: 18 SQRWWTCQPEGSVALRDRESMSAVPPLSVPTLLRQAARDAPDQLALAVKREGHWVHWSYAQYYADSRTAAKGFVALGLEPFHSVGILGFNAPEWFIAQNAAIMAHGFSAGIYTTNSPEACLHIALDCRANILVVEDEKQLDKILQIRPQLPDLK-----CVVQYLGTPHKEGVISWARLMELGAQESDDELERRLSAIAINQCCSLIYTSGTTGSPKGVMMSHDNFTWTARIANSFLKVTTEDSFISYLPLSHSAAQMIDVWMPMAARASVYFADRNALKGSLVNTLKEVRPTFFFGVPRIYEK----IQEKMVQIGKSSGAVKRLVADWAKRTGLQHNLRALEGQITPNQPGYSYPLAKKLVFSKVQENLGLDKCRIMGVGAAPMARETFEYFLSLDIPLLECYGMSETSGPQTGNRPGQHR---LGSIGPSLDGCKTKIFEADPDGHGEVLMRGRNIMMGYLFNEEKTREAIDDEGWLHSGDIGKELAESYFKITGRIKELIITAGGENVPPVLIEDVLKKELPCLSNTMVIGDRKKFLSCLLTLKVEVDVDTLEPKPELAAGALDWCKEIGSKAKTIDEAANDE--LVRKALQEGVDRTNQQATSNAQRIQKWHLLNHDFSVPGGELGPTLKLKRH 642 HSP 3 Score: 118.627 bits (296), Expect = 2.820e-27 Identity = 60/134 (44.78%), Postives = 85/134 (63.43%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNG-QPSTELSNLVKDWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG EN+ P+LIED +K LP LSN + IGD KKF+S LT KV D + +P EL+ DWC + +A T+ D+ V A+Q+G+D+ N+ A SNA +++KW L+ + S+ GE+GPTL
Sbjct: 506 KELIITAGGENVPPVLIEDVLKKELPCLSNTMVIGDRKKFLSCLLTLKVEVDVDTLEPKPELAAGALDWCKEIGSKAKTIDEAANDE--LVRKALQEGVDRTNQQATSNAQRIQKWHLLNHDFSVPGGELGPTL 637
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold764_size101305-processed-gene-0.1 (protein:Tk04090 transcript:snap_masked-scaffold764_size101305-processed-gene-0.1-mRNA-1 annotation:"hypothetical protein LOTGIDRAFT_234625") HSP 1 Score: 454.521 bits (1168), Expect = 4.574e-144 Identity = 261/641 (40.72%), Postives = 368/641 (57.41%), Query Frame = 0
Query: 11 DKMFPNKQALASSYTTTKPDKGVHIR---TESSEFLSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGEN--LILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYY-------ENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECG--IFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFH---------------TGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN-EDGEPTSELTDSVKKDFKNLGC--NYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
++MFP++ +TT+ + V +R T SVP ++ G ALA+ R G WVKW+YDQY +D++ AK FI+LGL+ Y V I FN PEWFI+ LGAI A G+S G+YPT+S Y++ +SR QILVVE+ L L+ +T LK +++ E +P EN + W D +++GR RL++RL I++N+CC L+YTSGTTG PKG MLSHD +T+ + + ++ + +VSYLPLSH+AA+ D+++ L G YFADK ALKGSL++T+KEV+PT F GVPRVWEK +E + E + K + KV K + +G + +K+ KL+F KVKQ LG +C +F S APL+ ++FLSLDI + E YG++EC C+ ++ G T K D I GEICIKGR VMMGYL +E T FDKEG+ TGR+K+L++T+GGEN+AP +E+ IK LP +SNAV++G+ KFIS LT+K + E P +L + K G + TTV + + + V + IQ GI+ AN A S A +VKKW LP + S+ GE+G TL
Sbjct: 7 EQMFPSE-----VFTTSDLAQAVKLRVSNTPPDNLPPISVPQLLTVSTNIGRDACALAVKRQGAWVKWSYDQYQADVRAAAKGFIQLGLETRYGVAIAGFNAPEWFIAQLGAIHAHGVSVGIYPTSSGEACRYILEHSRSQILVVEDQAQLDKFEPFLQELTYLKAVIMYEG---QPKENSSTLSWGDFIQMGRGVDDARLDERLQAIAINECCHLVYTSGTTGMPKGAMLSHDNLTWTGGQMSRIYNLNVKEERIVSYLPLSHVAANITDLFLILVSVGTAYFADKDALKGSLMNTVKEVQPTFFLGVPRVWEKIRERMLEVGQENKGLKQKVGNWAKRTGLETNMKRLSGDSSGAASLQYKLASKLVFSKVKQQLGFHKCTKFFSSAAPLAREVIEYFLSLDIRILEIYGMTECSGPHLCNT----YQMQKVGAYAGETVGFWNKIDGDQI-GEICIKGRHVMMGYLKDESKTRATFDKEGWLKSGDTGTRDSDGFVSITGRIKELIITAGGENIAPVLLEDNIKKELPCISNAVIIGEQRKFISCLLTLKTGIHPETMMPLDKLAPNTIAWIKEQGIEDDITTVSQAINNDL--VKEKIQAGIERANAIAPSRAQQVKKWVFLPEDFSVPGGELGPTL 632 HSP 2 Score: 384.8 bits (987), Expect = 2.742e-118 Identity = 232/611 (37.97%), Postives = 336/611 (54.99%), Query Frame = 0
Query: 658 DDLQPMSVPSFVKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGW-TKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQE----------GIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEF-----------------------------SCGGAPLMPT------------TTDFFLSLD------IVIK-----VAYGLSECGIFSDFAPSEY-KKGTCGSI----FPSTQGRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFK--VNSDTNGQPSTELSNSVKEWCHSLGYE--ASTVTNIIRDQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRN 1196
D+L P+SVP + + G A+A+KR G W++W+Y QY ++R AAK FI+LGL+ + V+I FN PEW I+ LGAI A G + G+YP++S Y++ +SR QILVVE+ L K +P L+ + +L K V++ E + ++ W D I++G+ ++ +L +RL IA N+CC ++YTSGTTG PKG M+SHD LT+T + + K+ IVSYLPLSH+AAN D+++++ G YFADK ALKGSL+ T+KEV+PT F GVPRV+EK +E G+++K+ K TN+ + +++G L +++++ K+K LG +C +F C G L T T F+ +D I IK + Y E + F + K G G+ F S GR KEL+ITAGGENIAP+L+EDNIK LP +SN V IG+ +KFIS LT K ++ +T P +L+ + W G E +TV+ I + V IQ GI+ AN I S A +VKKW +P + S+ GE+GPTLKLKR+
Sbjct: 34 DNLPPISVPQLLTVSTNIGRDACALAVKRQGAWVKWSYDQYQADVRAAAKGFIQLGLETRYGVAIAGFNAPEWFIAQLGAIHAHGVSVGIYPTSSGEACRYILEHSRSQILVVEDQAQLDKFEPFLQELTYL-KAVIMYEGQPKENSSTLSWGDFIQMGRGVDDARLDERLQAIAINECCHLVYTSGTTGMPKGAMLSHDNLTWTGGQMSRIYNLNVKEERIVSYLPLSHVAANITDLFLILVSVGTAYFADKDALKGSLMNTVKEVQPTFFLGVPRVWEKIRERMLEVGQENKGLKQKVGNWAKRTGLETNMKR---LSGDSSGAASLQYKLASKLVFSKVKQQLGFHKCTKFFSSAAPLAREVIEYFLSLDIRILEIYGMTECSGPHLCNTYQMQKVGAYAGETVGFWNKIDGDQIGEICIKGRHVMMGYLKDESKTRATFDKEGWLKSGDTGTRDSDGFVSITGRIKELIITAGGENIAPVLLEDNIKKELPCISNAVIIGEQRKFISCLLTLKTGIHPETM-MPLDKLAPNTIAWIKEQGIEDDITTVSQAINNDL--VKEKIQAGIERANAIAPSRAQQVKKWVFLPEDFSVPGGELGPTLKLKRH 637 HSP 3 Score: 100.523 bits (249), Expect = 1.217e-21 Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVN-SDKNGQPSTELSNLVKDWC--HSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
K+L ITAG ENIAP+L+ED IK LP +SN V IG+ +KFIS LT K + P +L+ W + + +TV+ I + ++ IQ GI++AN IA S A +VKKW +P + S+ GE+GPTL
Sbjct: 499 KELIITAGGENIAPVLLEDNIKKELPCISNAVIIGEQRKFISCLLTLKTGIHPETMMPLDKLAPNTIAWIKEQGIEDDITTVSQAINNDLVK--EKIQAGIERANAIAPSRAQQVKKWVFLPEDFSVPGGELGPTL 632
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold349_size200065-snap-gene-1.27 (protein:Tk10542 transcript:maker-scaffold349_size200065-snap-gene-1.27-mRNA-1 annotation:"amp dependent ligase") HSP 1 Score: 306.605 bits (784), Expect = 2.931e-90 Identity = 209/619 (33.76%), Postives = 317/619 (51.21%), Query Frame = 0
Query: 47 VPNMVKNHCLAGGSKTALAI-MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTI---SHILKNVTTLKKIVVIEEDGFRPGEN--LILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQK--VIHKIGDKLIFQKVKQALGLDRCLE---FISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAK-------VVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFK--NLGCNYTTVGEIL------YHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619
VP M+K G + AL+ + +G+W W + YY D++ A AF+ LGL V I + N PEW IS++ +I+AGGLS G+Y T+S T +Y+ NN+ ILV+EN L + + K + T+KKIV++E D +P + ++ WK L+ELGR L HD IT+ ++ + +++W K +++YLPLSH+AA +D Y++ VYFADK ALKGSL D L+EVRPT F VPRVWEK Q +++ + + ++Q K Y G K + + + KL+ +V QALGLDR + SG A L T ++F SL + +Q+ G +E Q ++ C P T K ++ D + GEI + R V MGY+ E T + F EG+F + GR+K+L+L SGG+N+AP PIE IK LP ++SN VVVG+ F++ +T +V+ + +PT+ L +SV K N+ TT +++ +H + + IQ G++ AN A S KV+K+ +LP+E S+ E+G TL
Sbjct: 62 VPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVTLTQYEW--EKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTET---TGPQTTNLAGPKCK---PGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHH----LRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTL 632 HSP 2 Score: 229.18 bits (583), Expect = 1.098e-63 Identity = 178/637 (27.94%), Postives = 300/637 (47.10%), Query Frame = 0
Query: 659 DLQPMSVPSFVKHNCLKGGSQIAMAIK-RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVL------QKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIK-----NLGNQMLYKKIKDSLGLDQC---QEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECG--IFSDFAPSEYKKGTCGSIF---------PSTQG------RKKELLITAGGENIAPI--------------------------------LIEDNIKNFLPV-------------LSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWC--HSLGYEASTVTNIIR--DQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR----NEFSKIHFNIYS 1207
+L P+ VP+ +K + + G + A++ K +G W WT+ YY ++R A AF+ LGL+ V I + N PEWMIS++ +I+AGG + G+Y ++S +Y+ NN+ ILV+EN L ++++ +L +K K+V++E D + S + W+ L+ELG RLH HD +T+T++ + ++ W K+ I++YLPLSH+AA +D Y+ +YFADK ALKGSL L+EVRPT F VPRV+EK Q +++ S+ +K I+ K Y +K L ++++ ++ +LGLD+ G A L T ++F SL + ++ G +E ++ A + K GT G + P + G R + + + E + I ++ KN P+ +SNVV +G+ + F++ +T +V D + QP+ L SV W +++ +T ++I + IQ G++MAN S +V+K+ ++P+E S+ E+GPTLKLKR +++K+ N+YS
Sbjct: 56 NLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVK---KIVLVETDIIQPHQKSFVMSWKQLVELG----------RLH--------------------------HDNITWTSRVTLTQYEWEKE-DILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652 HSP 3 Score: 75.0998 bits (183), Expect = 8.851e-14 Identity = 51/151 (33.77%), Postives = 85/151 (56.29%), Query Frame = 0
Query: 1304 VVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWC------HSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446
+++C R K+L + +G +NIAPI IE +IK+ LP ++SNVV +G+ + F++ +T +V D + QP+ L V W +++ + I + L I IQ G++ AN A S KV+K+ ++P+E S+ E+GPTL
Sbjct: 487 LIICGR---IKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQI--IQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTL 632
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold225_size250570-snap-gene-1.15 (protein:Tk01784 transcript:maker-scaffold225_size250570-snap-gene-1.15-mRNA-1 annotation:"long-chain-fatty-acid-- ligase acsbg2-like isoform 1") HSP 1 Score: 299.671 bits (766), Expect = 3.811e-86 Identity = 145/328 (44.21%), Postives = 220/328 (67.07%), Query Frame = 0
Query: 46 SVPNMVKNHCLAGGSKTALAIMR-NGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIE---EDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQS-KNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIF 368
S+P+ V+ +C + +AL + R +G +W Y QY+ DIK A+AFI+LGL+P ++V IY +N PEWFIS +GAI+AGG++ GLY TN TN+Y++ +++ ILVV N ++L I K++ LK I+ +E ++ + + +I W L++LG LNDRLS I++NQC LIYTSGTTG PKGVMLSHD I + T+ ++ +D+ + +VSYLPL+HIAA +D+++ L+ + ++FADK ALKG+L+ TL+E RPT FFGVPRVWEKF E I +Q K + K+ FK+A +++Y G +++ +GD +++
Sbjct: 127 SIPSQVRRYCESAKDHSALHVKREDGRTRRWTYYQYFIDIKRAARAFIELGLEPLHSVAIYGYNSPEWFISCVGAIYAGGIACGLYTTNGLETNKYILGDAKANILVVMNEEMLTQILPAKKDLPHLKAIIQMEGTVDEIYN--QEVISWTALMKLGEDASFSDLNDRLSEIAINQCAVLIYTSGTTGPPKGVMLSHDNIVYTTQTLMTMYDFKSQAETIVSYLPLNHIAAQIIDLWLGLFCESTIHFADKKALKGTLLTTLREARPTRFFGVPRVWEKFMEAISDQEVKESSGYRKKIVDYFKEAGVNHYIRGGTGLLYFLGDGVVY 452 HSP 2 Score: 280.026 bits (715), Expect = 1.813e-79 Identity = 147/360 (40.83%), Postives = 225/360 (62.50%), Query Frame = 0
Query: 636 IEDAKEYTTTLPDGSVIIRRKKD------DLQPMSVPSFVKHNCLKGGSQIAMAIKR-NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEED-GFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKI-SIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGI-EEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLY 985
I +T+ L DG V +R + + +P S+PS V+ C A+ +KR +G RWTY QY+ +I+ AA+AFI+LGL+P H+V+IY +N PEW IS +GAI+AGG A GLY +N N+Y++ +++ ILVV N +L ++ P +++ HL ++ +E I ++ I W L++LG+ L RL IA NQC +IYTSGTTG PKGVM+SHD + YT +T++ + + + +IVSYLPL+HIAA +D+++ + C ++FADK ALKG+L+ TL+E RPT FFGVPRV+EKF E I ++++ + +K I + K+A + G + LG+ ++Y
Sbjct: 94 IPTENRFTSDL-DGVVQLRLRDSATNPGINWRPTSIPSQVRRYCESAKDHSALHVKREDGRTRRWTYYQYFIDIKRAARAFIELGLEPLHSVAIYGYNSPEWFISCVGAIYAGGIACGLYTTNGLETNKYILGDAKANILVVMNEEMLTQILPAKKDLPHLKAIIQMEGTVDEIYNQEVISWTALMKLGEDASFSDLNDRLSEIAINQCAVLIYTSGTTGPPKGVMLSHDNIVYTTQTLMTMYDFKSQAETIVSYLPLNHIAAQIIDLWLGLFCESTIHFADKKALKGTLLTTLREARPTRFFGVPRVWEKFMEAISDQEVKESSGYRKKIVDYFKEAGVNHYIRGGTGLLYFLGDGVVY 452 HSP 3 Score: 184.882 bits (468), Expect = 5.006e-48 Identity = 113/288 (39.24%), Postives = 159/288 (55.21%), Query Frame = 0
Query: 364 DKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSE-CGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIK-VKSNEDGE-PTSELTDSVKKDF-KNLGCNYTTVGEILYHKPPGVMKAIQKGIDE--------------ANKRAISNAAKVKKWCILPRELSLEAGEIGIT 618
DK IF K+ALGL+RC I G A L R FF LDI + +CYG+SE CG+ +F G+ G + +++ D GE+CI GR VMMGY+ + T EA G+ TG R+KD+++TSGG+NV+P+PIE+ IK LP +SNAVV+GD +K++ LT+K + ED + P S L + D+ +++G T+GEIL +K V +AIQ ID N A+SNA KV++W ILP++LS GE+G T
Sbjct: 533 DKKIFPVDKKALGLNRCSTMICGAATLPNEVRRFFFGLDINILDCYGMSETCGLGTMSVHDEFAFGSIGMAVSGLEMRLI-DRDASGNGELCIWGRNVMMGYVNQLEKTDEALTSTGFIRTGDIANCDLNDFFYLSGRVKDIIVTSGGDNVSPSPIEDGIKSLLPCLSNAVVIGDQKKYLIAFLTLKTLPDAEDPDIPYSNLLTRLTLDWCRSVGSVAQTLGEILKYKDESVFRAIQDVIDAVRMELISRKLEPTWVNINAMSNATKVQRWTILPKDLSTAGGELGPT 819 HSP 4 Score: 110.923 bits (276), Expect = 1.104e-24 Identity = 62/161 (38.51%), Postives = 91/161 (56.52%), Query Frame = 0
Query: 1056 GRKKELLITAGGENIAPILIEDNIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDTNGQPSTELSNSVK----EWCHSLGYEASTVTNIIRDQPPEVMSAIQKGIDM--------------ANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKRNEF 1198
GR K++++T+GG+N++P IED IK+ LP LSN V IGD KK++ FLT K D P SN + +WC S+G A T+ I++ + V AIQ ID N +SNA +V++W+++P++LS GE+GPT K +R F
Sbjct: 668 GRVKDIIVTSGGDNVSPSPIEDGIKSLLPCLSNAVVIGDQKKYLIAFLTLKTLPDAE-DPDIPYSNLLTRLTLDWCRSVGSVAQTLGEILKYKDESVFRAIQDVIDAVRMELISRKLEPTWVNINAMSNATKVQRWTILPKDLSTAGGELGPTGKFRRFFF 827 HSP 5 Score: 103.605 bits (257), Expect = 1.881e-22 Identity = 60/150 (40.00%), Postives = 83/150 (55.33%), Query Frame = 0
Query: 1314 KQLFITAGVENIAPILIEDKIKNFLPVLSNVVAIGDLKKFISVFLTFKVNSDKNGQPSTELSNLVK----DWCHSLSYEASTVTNIIRDKPLEVISAIQKGIDK--------------ANKIAVSNAAKVKKWSLIPRELSMDNGEMGPT 1445
K + +T+G +N++P IED IK+ LP LSN V IGD KK++ FLT K D P SNL+ DWC S+ A T+ I++ K V AIQ ID N A+SNA KV++W+++P++LS GE+GPT
Sbjct: 671 KDIIVTSGGDNVSPSPIEDGIKSLLPCLSNAVVIGDQKKYLIAFLTLKTLPDAE-DPDIPYSNLLTRLTLDWCRSVGSVAQTLGEILKYKDESVFRAIQDVIDAVRMELISRKLEPTWVNINAMSNATKVQRWTILPKDLSTAGGELGPT 819
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold958_size76609-snap-gene-0.4 (protein:Tk08629 transcript:maker-scaffold958_size76609-snap-gene-0.4-mRNA-1 annotation:"long-chain fatty acid-- ligase") HSP 1 Score: 143.665 bits (361), Expect = 3.164e-36 Identity = 111/446 (24.89%), Postives = 200/446 (44.84%), Query Frame = 0
Query: 669 VKHNCLKGGSQIAMAIKRNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADK-LSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLSLDIVIKVAY---------GLSECGIFSDFAPSEYKKGTCGSIFPSTQGRKKELLITAGGENIAPILIEDNI-KNFLPVLSNVVAIGDLKKFISVFL 1103
++ +GG++ A+ K + W ++ Q+ ++I + A + GL+ + I+S N P+W +++ ++ A +YP+N+A+ + Y++ ++ +IL V + E + L VV + D + ++ W+D + G ++ +L RL + + ++IYTSGTTG PKGVM+ + + Y K E+ TK + +LPLSH+ A Y + Y D ++ + L +V+PTV VPR YEK I EK+ + +F I + Y T + ++ DS+G+ S GA + D L ++ Y E G + + G++F + R KEL+ T+GG+ IAP ++E I K+ + + I D +KF+S +
Sbjct: 12 LRDQIAQGGNRTALKHKVDNVWQGISWEQFGQQIDTLSLALLAHGLRVQDKIGIFSNNMPQWTVADFASLQARLVTVPIYPTNTAAQSSYIIQDADVKILFVGEQAQFDAAVSLFEECEQLEVVVAMSADIDTQEHSFAVSWQDFMARGVEAQQAELDVRLADANMDDLLTLIYTSGTTGQPKGVMLDYTNIGYQLKGHDERLSLTKDDVSLCFLPLSHVFERAWTFYALYKGATNCYLQDTMQVRDA----LSDVKPTVMCAVPRFYEKIFSAIHEKLD--ETIGRFFHAIGINVKLGYGMTETTATVSCWDDRCFN---PDSIGM------SMPGAEVKIGAKDEILVRGPMVMRGYYKMPEETAKTFDEHGFLKTGDAGHFDEN--GNLFITD--RIKELMKTSGGKYIAPQVVEGAIGKDHF--IEQIAVIADTRKFVSALI 436 HSP 2 Score: 120.553 bits (301), Expect = 1.511e-28 Identity = 81/292 (27.74%), Postives = 144/292 (49.32%), Query Frame = 0
Query: 43 LSFSVPNMVKNHCLAGGSKTALAIMRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEED-GFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWT---TAKFIVVSYLPLSHIAASFLDIYVALYLKGEV--YFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQ 328
L F + +++ GG++TAL + W +++Q+ I + A + GL + I+S N P+W +++ ++ A ++ +YPTN+++ + Y++ ++ +IL V + + L+ +V + D + + W+D + G + L+ RL+ +++ +LIYTSGTTG PKGVML + I + +K HD T + + +LPLSH+ Y ALY KG Y D ++ D L +V+PT+ VPR +EK I E+
Sbjct: 4 LDFHIVKRLRDQIAQGGNRTALKHKVDNVWQGISWEQFGQQIDTLSLALLAHGLRVQDKIGIFSNNMPQWTVADFASLQARLVTVPIYPTNTAAQSSYIIQDADVKILFVGEQAQFDAAVSLFEECEQLEVVVAMSADIDTQEHSFAVSWQDFMARGVEAQQAELDVRLADANMDDLLTLIYTSGTTGQPKGVMLDYTNIGYQ----LKGHDERLSLTKDDVSLCFLPLSHVFERAWTFY-ALY-KGATNCYLQDTMQVR----DALSDVKPTVMCAVPRFYEKIFSAIHEK 285 HSP 3 Score: 62.003 bits (149), Expect = 8.160e-10 Identity = 54/219 (24.66%), Postives = 95/219 (43.38%), Query Frame = 0
Query: 390 LSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSD-FKTGTCGTIIPSTHAKVVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTG---------------RLKDLLLTSGGENVAPAPIENAI--KHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKR 590
L E FF ++ I V+ YG++E S F + G +P K+ EI ++G VM GY + T++ FD+ G+ TG R+K+L+ TSGG+ +AP +E AI H++ ++ V+ D KF+S + DS+++ K L Y E++ H +++ ++K +++ K
Sbjct: 286 LDETIGRFFHAIGINVKLGYGMTETTATVSCWDDRCFNPDSIGMSMPGAEVKIGAKD------EILVRGPMVMRGYYKMPEETAKTFDEHGFLKTGDAGHFDENGNLFITDRIKELMKTSGGKYIAPQVVEGAIGKDHFIEQIA---VIADTRKFVSALIV-------------PCYDSLEEYAKELNIKYHDRVELVKHHQ--IVEMLEKRVNDLQKE 480
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold144_size312663-processed-gene-0.0 (protein:Tk07263 transcript:snap_masked-scaffold144_size312663-processed-gene-0.0-mRNA-1 annotation:"long-chain-fatty-acid-- ligase 4") HSP 1 Score: 134.42 bits (337), Expect = 9.666e-32 Identity = 134/518 (25.87%), Postives = 239/518 (46.14%), Query Frame = 0
Query: 69 NGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYT-VCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLND------RLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKA-IVKKHDWT----TAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYAL--KGSLVDTLKE-----VRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLS-LDIPVQECYGLSEC-GIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSD------DDIG--GEICIKGRCVMMGYLFNEQATSEAF--DKEG--YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN 538
G++ NY+Q AK + +G++P IY+ W ++ +G G A LY + ++ +N ++ + L++ + L + + ++ L+ I+ I R + + ++L E I RL + RL+ + ++YTSG+TG PKG +L+H T KA +V+ D A ++LP++HI +V L + ++ ++ L K S++ ++ ++PT+ GVP + ++ + I + + K V Q + + + G I ++L+F K+KQ+LG R IS GAPLS +F + LD+ + + YGL+E G C +S+ +TG G +P K++ S+ D G GEI + G V GY + T+E+F D+EG +F+TG R KDL+ + GE V+ +E A+K + PVV N V D+ K ++ + + ++N
Sbjct: 580 QGDYQWINYEQVDDISTNIAKGMLAIGMNPKEDKAIIYADTSANWLMNCIGCFKTGITLATLYTNLGDESVKFGINQTQAKTLIMSQ-EFLPKVKRYIDDLPHLEHIIYIPS---RVKKTVDTKFSKVKLHSMEDIQRLGENAPSHIRLTPPKADDLAMIMYTSGSTGTPKGALLTHWTFTMAIKACMVRALDLAGEQRYADESFFAFLPIAHI-FELSHEFVVLLMGVKIGYSGPNTLTDKSSMIKAGQKGDATVLKPTIMLGVPTILDRIYKAIHSNVQAKGKVFEAVFQKLYQYRLSWVKKGFGT---PIINRLVFSKLKQSLG-GRIELIISAGAPLSADVHEFIRTCLDVHIAQGYGLTETMGGVCLSDRSELETGQVGFPLPGVMMKLINWSEGGYSVRDPQGPRGEIVLGGPNVSTGYFDMPEKTAESFEVDQEGNQWFYTGDIGHLLPRGTIRIIDRKKDLVKLAMGEYVSLGKVEAAMKVH-PVVDNICVYADSSKTFTIAIVVAEENN 1087 HSP 2 Score: 80.4925 bits (197), Expect = 2.893e-15 Identity = 97/391 (24.81%), Postives = 171/391 (43.73%), Query Frame = 0
Query: 687 NGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSI-YSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIE-------EDGFIADKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKT----VVEKHGWTKKI--SIVSYLPLSHIAANALDIYVV-----ICCRGELYFADKSAL-KGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKK-FIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLS-LDIVIKVAYGLSEC--GI-FSDFAPSEYKKGTCGSIFP 1052
GD+ Y Q AK + +G+ P +I Y+ W+++ +G G LY + ++ +N ++ + L++ + L KV+ ++++ HL ++ I + F KL + D+ LG E RL A+ I+YTSG+TG PKG +++H T K ++ G + S ++LP++HI + + V+ I G DKS++ K ++PT+ GVP + ++ + I + K + + L + Y + KK F + N++++ K+K SLG + + GAPL +F + LD+ I YGL+E G+ SD SE + G G P
Sbjct: 580 QGDYQWINYEQVDDISTNIAKGMLAIGMNPKEDKAIIYADTSANWLMNCIGCFKTGITLATLYTNLGDESVKFGINQTQAKTLIM-SQEFLPKVKRYIDDLPHLEHIIYIPSRVKKTVDTKFSKVKLHSM-EDIQRLG---ENAPSHIRLTPPKADDLAMIMYTSGSTGTPKGALLTHWTFTMAIKACMVRALDLAGEQRYADESFFAFLPIAHIFELSHEFVVLLMGVKIGYSGPNTLTDKSSMIKAGQKGDATVLKPTIMLGVPTILDRIYKAIHSNVQAKGKVFEAVFQKL----YQYRLSWVKKGFGTPIINRLVFSKLKQSLG-GRIELIISAGAPLSADVHEFIRTCLDVHIAQGYGLTETMGGVCLSD--RSELETGQVGFPLP 958
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold179_size282488-processed-gene-1.19 (protein:Tk10715 transcript:snap_masked-scaffold179_size282488-processed-gene-1.19-mRNA-1 annotation:"GI18805") HSP 1 Score: 124.405 bits (311), Expect = 4.767e-29 Identity = 138/566 (24.38%), Postives = 245/566 (43.29%), Query Frame = 0
Query: 34 HIRTESSEFLSFSVPNMVKNHCLAGGSKTALAI-------------MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEE-------DGFRPGENLILWKDLLELGRKEGIDRLNDRLS-HISVNQCCSLIYTSGTTGNPKGVMLSH-DIITFMTKAIV---KKHDWTTAKFIVVSYLPLSHI-------AASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLS-LDIPVQECYGLSECGIFCSV-QKSDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAF--DKEG--YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN 538
H+ T + F ++SV + CL G++ L I + G++ +YDQ ++ + + LGL P + +++ EW I LGA F+ + G TN + N +VV + ++L S IL + +++ ++ +E+ DG+ G + + ++ +G++++ + + + ++YTSG+TG PKGV+L+H +++ M+ + K D I ++YLPL+H+ L I V + KG+ D ++PTM VP + ++ + + + K + + KV + ++ G I D+++F K++ LG R + GGAPL + T DF + L + V + Y L+E G +V + D TGT GT + K++ + GEIC+ G V GY + T F D +G +F TG R KDL+ GE V+ +E +K + V N V GD+ K +V L + K++
Sbjct: 106 HVNTVTQAF-NYSVRKFGRQACL--GTREILGIEDEVQQNGKLFQKLSLGDYQWLSYDQVHAQAQAFGRGLRSLGLAPGAKIAMFAETRAEWMIGCLGA-FSQNIHVGTVYTNLGNDAVIHAINEIEVSVVVTSHELLPKFSSILPSCASVRHVLYMEDPVQTTKTDGYLAGVQITPFAHIV----SDGMEKVEQFAAVEPTPDDTAIIMYTSGSTGTPKGVVLTHRNLVATMSCLMFMLNPKDD------IYIAYLPLAHVLELLSENTMMLLGIRVGYSSPNTMTDMSTKIRKGTKGDA-SVLQPTMMCAVPLILDRIYKNVLDSVKKRGPTFQKVFNFCYEYKRYWMRQGRGT---PICDRIVFNKIRALLG-GRMGFVLVGGAPLCQETHDFIRTCLGVMVVQGYSLTESGCTGTVMENRDLSTGTVGTPMTGLEVKLINWEEGSYTVTDKPRPRGEICLSGVPVAKGYYKQDAETQSNFFEDNKGKRWFKTGDIGEFNEHGQLSIVDRKKDLVKLQLGEYVSLGKVEAQMKTH-SAVENICVYGDSNKHSTVALVVPSKAH 651 HSP 2 Score: 74.3294 bits (181), Expect = 1.606e-13 Identity = 88/371 (23.72%), Postives = 160/371 (43.13%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGA----IFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXV--LQKVQPILENIKHLL----KVVVIEEDGFIADKLSILWRDLIELG--KTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHI-------AANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLG------NQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLS-LDIVIKVAYGLSECG 1032
GD+ +Y Q + + + + LGL P +++++ EWMI LGA I G T L N A + + +N ++V + + + P +++H+L V + DG++A + ++ G K E+ ++ + A I+YTSG+TG PKGV+++H L T ++ I I +YLPL+H+ L I V + KG+ ++PT+ VP + ++ + + + + K + + + N FCY K++ G +++++ KI+ LG + GGAPL T DF + L +++ Y L+E G
Sbjct: 152 GDYQWLSYDQVHAQAQAFGRGLRSLGLAPGAKIAMFAETRAEWMIGCLGAFSQNIHVGTVYTNL--GNDAVI--HAINEIEVSVVVTSHELLPKFSSILPSCASVRHVLYMEDPVQTTKTDGYLAGVQITPFAHIVSDGMEKVEQFAAVEPTPDDTAI-----IMYTSGSTGTPKGVVLTHRNLVATMSCLMFMLNPKDDIYI-AYLPLAHVLELLSENTMMLLGIRVGYSSPNTMTDMSTKIRKGT-KGDASVLQPTMMCAVPLILDRIYKNVLDSVKKRGPTFQKVFN------FCYEY---KRYWMRQGRGTPICDRIVFNKIRALLG-GRMGFVLVGGAPLCQETHDFIRTCLGVMVVQGYSLTESG 501
BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold566_size135349-processed-gene-0.9 (protein:Tk08884 transcript:snap_masked-scaffold566_size135349-processed-gene-0.9-mRNA-1 annotation:"GI18805") HSP 1 Score: 124.405 bits (311), Expect = 4.767e-29 Identity = 138/566 (24.38%), Postives = 245/566 (43.29%), Query Frame = 0
Query: 34 HIRTESSEFLSFSVPNMVKNHCLAGGSKTALAI-------------MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEE-------DGFRPGENLILWKDLLELGRKEGIDRLNDRLS-HISVNQCCSLIYTSGTTGNPKGVMLSH-DIITFMTKAIV---KKHDWTTAKFIVVSYLPLSHI-------AASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLS-LDIPVQECYGLSECGIFCSV-QKSDFKTGTCGTIIPSTHAKVVKSSDDDIG--------GEICIKGRCVMMGYLFNEQATSEAF--DKEG--YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSN 538
H+ T + F ++SV + CL G++ L I + G++ +YDQ ++ + + LGL P + +++ EW I LGA F+ + G TN + N +VV + ++L S IL + +++ ++ +E+ DG+ G + + ++ +G++++ + + + ++YTSG+TG PKGV+L+H +++ M+ + K D I ++YLPL+H+ L I V + KG+ D ++PTM VP + ++ + + + K + + KV + ++ G I D+++F K++ LG R + GGAPL + T DF + L + V + Y L+E G +V + D TGT GT + K++ + GEIC+ G V GY + T F D +G +F TG R KDL+ GE V+ +E +K + V N V GD+ K +V L + K++
Sbjct: 106 HVNTVTQAF-NYSVRKFGRQACL--GTREILGIEDEVQQNGKLFQKLSLGDYQWLSYDQVHAQAQAFGRGLRSLGLAPGAKIAMFAETRAEWMIGCLGA-FSQNIHVGTVYTNLGNDAVIHAINEIEVSVVVTSHELLPKFSSILPSCASVRHVLYMEDPVQTTKTDGYLAGVQITPFAHIV----SDGMEKVEQFAAVEPTPDDTAIIMYTSGSTGTPKGVVLTHRNLVATMSCLMFMLNPKDD------IYIAYLPLAHVLELLSENTMMLLGIRVGYSSPNTMTDMSTKIRKGTKGDA-SVLQPTMMCAVPLILDRIYKNVLDSVKKRGPTFQKVFNFCYEYKRYWMRQGRGT---PICDRIVFNKIRALLG-GRMGFVLVGGAPLCQETHDFIRTCLGVMVVQGYSLTESGCTGTVMENRDLSTGTVGTPMTGLEVKLINWEEGSYTVTDKPRPRGEICLSGVPVAKGYYKQDAETQSNFFEDNKGKRWFKTGDIGEFNEHGQLSIVDRKKDLVKLQLGEYVSLGKVEAQMKTH-SAVENICVYGDSNKHSTVALVVPSKAH 651 HSP 2 Score: 74.3294 bits (181), Expect = 1.606e-13 Identity = 88/371 (23.72%), Postives = 160/371 (43.13%), Query Frame = 0
Query: 688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGA----IFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXV--LQKVQPILENIKHLL----KVVVIEEDGFIADKLSILWRDLIELG--KTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHI-------AANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIKNLG------NQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLS-LDIVIKVAYGLSECG 1032
GD+ +Y Q + + + + LGL P +++++ EWMI LGA I G T L N A + + +N ++V + + + P +++H+L V + DG++A + ++ G K E+ ++ + A I+YTSG+TG PKGV+++H L T ++ I I +YLPL+H+ L I V + KG+ ++PT+ VP + ++ + + + + K + + + N FCY K++ G +++++ KI+ LG + GGAPL T DF + L +++ Y L+E G
Sbjct: 152 GDYQWLSYDQVHAQAQAFGRGLRSLGLAPGAKIAMFAETRAEWMIGCLGAFSQNIHVGTVYTNL--GNDAVI--HAINEIEVSVVVTSHELLPKFSSILPSCASVRHVLYMEDPVQTTKTDGYLAGVQITPFAHIVSDGMEKVEQFAAVEPTPDDTAI-----IMYTSGSTGTPKGVVLTHRNLVATMSCLMFMLNPKDDIYI-AYLPLAHVLELLSENTMMLLGIRVGYSSPNTMTDMSTKIRKGT-KGDASVLQPTMMCAVPLILDRIYKNVLDSVKKRGPTFQKVFN------FCYEY---KRYWMRQGRGTPICDRIVFNKIRALLG-GRMGFVLVGGAPLCQETHDFIRTCLGVMVVQGYSLTESG 501 The following BLAST results are available for this feature:
BLAST of EMLSAG00000003336 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO) Total hits: 25
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BLAST of EMLSAG00000003336 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA) Total hits: 25
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BLAST of EMLSAG00000003336 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self) Total hits: 11
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BLAST of EMLSAG00000003336 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt) Total hits: 25
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BLAST of EMLSAG00000003336 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods) Total hits: 25
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BLAST of EMLSAG00000003336 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017)) Total hits: 25
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BLAST of EMLSAG00000003336 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins) Total hits: 24
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Cross References
External references for this gene
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at LSalAtl2s182:521806..564306+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>EMLSAG00000003336-686102 ID=EMLSAG00000003336-686102|Name=EMLSAG00000003336|organism=Lepeophtheirus salmonis|type=gene|length=42501bp|location=Sequence derived from alignment at LSalAtl2s182:521806..564306+ (Lepeophtheirus salmonis)back to top Add to Basket
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