EMLSAG00000004167, EMLSAG00000004167-686933 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000004167
Unique NameEMLSAG00000004167-686933
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:Agrn "agrin" species:10090 "Mus musculus" [GO:0001932 "regulation of protein phosphorylation" evidence=ISO] [GO:0001934 "positive regulation of protein phosphorylation" evidence=IDA] [GO:0002162 "dystroglycan binding" evidence=ISO] [GO:0005509 "calcium ion binding" evidence=ISO] [GO:0005515 "protein binding" evidence=IPI] [GO:0005604 "basement membrane" evidence=ISO;IDA] [GO:0005605 "basal lamina" evidence=ISO;IDA] [GO:0005615 "extracellular space" evidence=IDA;TAS] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005886 "plasma membrane" evidence=ISO] [GO:0007009 "plasma membrane organization" evidence=IMP] [GO:0007268 "synaptic transmission" evidence=ISO;IDA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0007416 "synapse assembly" evidence=ISO] [GO:0007528 "neuromuscular junction development" evidence=IGI] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IMP] [GO:0009986 "cell surface" evidence=IDA] [GO:0010469 "regulation of receptor activity" evidence=IDA] [GO:0016020 "membrane" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IDA] [GO:0031012 "extracellular matrix" evidence=ISO] [GO:0032092 "positive regulation of protein binding" evidence=IDA] [GO:0032314 "regulation of Rac GTPase activity" evidence=IDA] [GO:0032321 "positive regulation of Rho GTPase activity" evidence=ISO] [GO:0035374 "chondroitin sulfate binding" evidence=IDA] [GO:0043113 "receptor clustering" evidence=IGI;ISO;IMP;IDA] [GO:0043395 "heparan sulfate proteoglycan binding" evidence=IDA] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IMP] [GO:0045202 "synapse" evidence=ISO;IDA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IDA] [GO:0045887 "positive regulation of synaptic growth at neuromuscular junction" evidence=IMP] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=ISO;IDA] [GO:0051290 "protein heterotetramerization" evidence=ISO] [GO:0051491 "positive regulation of filopodium assembly" evidence=ISO] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] [GO:2000541 "positive regulation of protein geranylgeranylation" evidence=IMP] InterPro:IPR000742 InterPro:IPR002350 Pfam:PF00008 Pfam:PF00050 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 MGI:MGI:87961 GO:GO:0016021 GO:GO:0007275 GO:GO:0005886 GO:GO:0005615 GO:GO:0009986 GO:GO:0030154 GO:GO:0005509 GO:GO:0043525 GO:GO:0032092 GO:GO:0030054 GO:GO:0045202 GO:GO:0007528 GO:GO:0045887 GO:GO:0045944 GO:GO:0007009 Reactome:REACT_188937 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 Reactome:REACT_189085 PROSITE:PS01186 GO:GO:0033691 GO:GO:0002162 eggNOG:NOG312635 HOGENOM:HOG000033860 HOVERGEN:HBG080471 GO:GO:0030548 GO:GO:0035374 GO:GO:0043395 GO:GO:0051491 GO:GO:0032321 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OrthoDB:EOG7BGHJZ TreeFam:TF326548 GO:GO:0005796 GO:GO:0045213 EMBL:AL928667 EMBL:BC150703 UniGene:Mm.273098 PDB:3PVE PDBsum:3PVE ProteinModelPortal:A2ASQ1 SMR:A2ASQ1 PaxDb:A2ASQ1 PRIDE:A2ASQ1 Ensembl:ENSMUST00000105575 UCSC:uc008wgf.2 UCSC:uc008wgg.1 GeneTree:ENSGT00530000063501 InParanoid:A2ASQ1 Reactome:REACT_194409 ChiTaRS:AGRN EvolutionaryTrace:A2ASQ1 PRO:PR:A2ASQ1 ArrayExpress:A2ASQ1 Bgee:A2ASQ1 Genevestigator:A2ASQ1 GO:GO:2000541 GO:GO:0001934 GO:GO:0032314 Uniprot:A2ASQ1)

HSP 1 Score: 136.732 bits (343), Expect = 4.353e-31
Identity = 111/385 (28.83%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    FSC C +G  G+ CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  +          H+ F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL   L++GG+       +  V       IG  GCI+ L IN + + L   +   V++  G+ EC ++ CS NPC    LC +   G FLC C     G +C   +N  C PNPC  +  CH+  RG   C C      G++C+    +    +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1215 CDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCE------KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDG---------HVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQ---VATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQR-AVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNP-CQPNPCHGSAPCHVLSRGGAKCACPLGR-SGSFCE-TVLENAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1558          

HSP 2 Score: 124.02 bits (310), Expect = 3.068e-27
Identity = 119/397 (29.97%), Postives = 172/397 (43.32%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            CR + C FGA+C V  K+G + C+C+ + CSG  + PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +T      C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +     G C   +         +   C +  C   G V +EDS    CVC  +C+   + P      VCG DG TY TE  L++  C+ ++E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CRGAQCAFGATCTV--KNGKAVCECQRV-CSGGYD-PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDR---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGETV-----CTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQ--VQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSP------VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPT-LAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPVTG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.5153 bits (236), Expect = 1.822e-18
Identity = 86/317 (27.13%), Postives = 122/317 (38.49%), Query Frame = 0
Query:  201 NCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE-EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSP 515
             C+F + C+  ++ G  +C C  + C G   +PVC  D  TY N+C   QA C ++  +      PC++    C    C FG +C                                                    + K+GK++C C    S G    DPVCG+D +TY SVC L +  C+   G ++RV   G C          CG+  C FG++CE E   CVC   C E  Q        VCG DG TY +E +L  HAC  Q  + VA    C      +  +            + C   +C+CP    P
Sbjct:  293 TCQFNSVCL--SRRGRPHCSCDRVTCDGAY-RPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATC----------------------------------------------------TVKNGKAVCECQRVCSGGY---DPVCGSDGVTYGSVCELESMACT--LGREIRVARRGPCDR--------CGQ--CRFGSLCEVETGRCVCPSECVESAQ-------PVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTF-----------GAVCSAGQCVCPRCEHP 521          

HSP 4 Score: 93.9745 bits (232), Expect = 5.826e-18
Identity = 64/233 (27.47%), Postives = 109/233 (46.78%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  +   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I              +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1710 NPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGR-TYIEY---LNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1934          

HSP 5 Score: 90.5077 bits (223), Expect = 6.699e-17
Identity = 83/296 (28.04%), Postives = 127/296 (42.91%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C PNPC G   C     G   C C  G +GS CE  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L++GG      P   K+ + A  + GF G I+ + +    +  Q      V+R   +     + C+    NPC N G CI +   + C C   F+G  C++
Sbjct: 1474 CQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGA-----PDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEH----VLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEK 1740          

HSP 6 Score: 86.2705 bits (212), Expect = 1.313e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCV------CSVTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC ++  ++       C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +            C++++  P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC R G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQ-EHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGETVCTFGAVCSAGQCVCPRCEH 520          

HSP 7 Score: 59.3066 bits (142), Expect = 2.106e-7
Identity = 81/303 (26.73%), Postives = 112/303 (36.96%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISD-----------LPCEEAHLC-----------------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            + D  S D  C C    C   +  PVCGSD +TY  EC L +A C  R  L V +            LP    H                   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +      C       P+ C +  C+FGA C E++    CVC ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I +    PC
Sbjct:  588 VWDEDSEDGPCVCD-FSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPVTGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQALGHLGCEA-DPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTC------PEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPC 864          

HSP 8 Score: 56.9954 bits (136), Expect = 1.133e-6
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+ +A  G + C C  + C    +  VCGSD +TY NEC L    C +R+++++ S  PC E+
Sbjct:  796 CVEMHCEFGASCVEEA--GFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRES 867          

HSP 9 Score: 56.225 bits (134), Expect = 2.065e-6
Identity = 96/410 (23.41%), Postives = 149/410 (36.34%), Query Frame = 0
Query:  198 RHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFG-----GSCV----------RTGGNNNKIYKSQCHMELDACESRL----------------TNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVC--SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCL--------ALNSEC--VNNRCICPEGTSPNSANTSCEIMKIGSL-CDPNP----------CQGGGTCDEHDGTFSCYC 553
            R   CRFG+ C V+  +G   C  + ++ +    +PVCGSD  TY +EC+L    CT +I+L V S   C+    CG   C FG     G CV             G++   Y S C +   AC+ ++                +  + S  DN  +C Q        ++ E S    C C    S  +  + PVCG+D +TY + C L+ + C  ++  ++ V   G CR       +P     C       +  Y  C  +VT  +        +   C   G +Y    D     C  +  +       C  G            S C         A+  +C  +   C C  G +        +   +G L C+ +P          C+ G +C E  G   C C
Sbjct:  431 RCGQCRFGSLCEVE--TGRCVCPSECVESA----QPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQT---CGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDN--ACEQELCRQHGGVWDEDSEDGPCVCDF--SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQ--ELYVAAQGACRGPTLAPLLPMASPHC------AQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHG-SYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVC 818          

HSP 10 Score: 53.5286 bits (127), Expect = 1.101e-5
Identity = 54/224 (24.11%), Postives = 83/224 (37.05%), Query Frame = 0
Query:  189 SSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSC 412
            +S  + + C  + C FGA C     +G   C      C      PVCGSD +TY + C+L +A C +++ +      PCE A                               E +ACE  L       H           W+ED     + G  +C      S  +  + PVCG+D +TY + C L+ + C  ++  ++ V   G CR       +P     C
Sbjct:  489 ASAGHCQTCGETVCTFGAVC----SAGQCVCP----RCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAEC---------------------GSGGSGSGEDNACEQELCR----QHGG--------VWDED----SEDGPCVCDF----SCQSVLKSPVCGSDGVTYSTECHLKKARCEARQ--ELYVAAQGACRGPTLAPLLPMASPHC 661          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:Agrn "Agrin" species:10116 "Rattus norvegicus" [GO:0005605 "basal lamina" evidence=IEA] [GO:0005615 "extracellular space" evidence=IEA] [GO:0007009 "plasma membrane organization" evidence=IEA] [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IEA] [GO:0009986 "cell surface" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IEA] [GO:0043113 "receptor clustering" evidence=IEA] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IEA] [GO:0045202 "synapse" evidence=IEA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000742 InterPro:IPR002350 Pfam:PF00008 Pfam:PF00050 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 RGD:2067 GO:GO:0005615 GO:GO:0009986 GO:GO:0043525 GO:GO:0045202 GO:GO:0007528 GO:GO:0045944 GO:GO:0007009 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 PROSITE:PS01186 GO:GO:0030548 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OMA:PRCSCDR OrthoDB:EOG7BGHJZ TreeFam:TF326548 GO:GO:0045213 GO:GO:0010469 GeneTree:ENSGT00530000063501 UniGene:Rn.2163 GeneID:25592 GO:GO:0008582 EMBL:AABR06041320 RefSeq:XP_006239605.1 Ensembl:ENSRNOT00000045678 NextBio:35576110 Uniprot:F1LPF2)

HSP 1 Score: 127.872 bits (320), Expect = 1.879e-28
Identity = 109/385 (28.31%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    F+C C +G  GS CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  + +         + F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL  +L++GG+ P +   M   R + G  +G  GCI+ L IN + + L   +    ++  G+ EC ++ C  NPC    LC +   G FLC C     G +C   ++  C PNPC     C +   G   C C      GT+C     +T   +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1224 CDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCE------KVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDGR---------VQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGI-PEEQVAMVLDRTSVG--VGLKGCIRMLDINNQQLELSDWQR-AAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSP-CQPNPCHGAAPCRVLSSGGAKCECPLGR-SGTFCQ-TVLETAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1567          

HSP 2 Score: 121.324 bits (303), Expect = 2.035e-26
Identity = 119/397 (29.97%), Postives = 171/397 (43.07%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA C V  K+G + C+C+ + CSG I  PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +     + C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +   + G C   +         +   C +  C   G + +EDS    CVC  +C+   + P      VCG DG TY TE DL++  C+ Q+E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CHGVQCAFGAVCTV--KNGKAECECQRV-CSG-IYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDP---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGE-----KVCTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQV--QIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSP------VCGSDGVTYGTECDLKKARCESQQELYVAAQGAC-RGPT-LAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPATG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.9005 bits (237), Expect = 1.391e-18
Identity = 65/233 (27.90%), Postives = 111/233 (47.64%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  L   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I  ++          +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1719 NPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGR-TYIEY---LNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1943          

HSP 4 Score: 94.7449 bits (234), Expect = 3.221e-18
Identity = 90/320 (28.12%), Postives = 137/320 (42.81%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C CP    P     +C   K  S C PNPC G   C     G   C C  G +G+ C+  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L+IGG      P   K+ + A  S GF G I+ + +    +  Q      V+R   +    ++ C+   GNPC N G C+ +   + C C   F+G  C++
Sbjct: 1465 CQCP----PGRFGPTCADEK--SPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA-----PDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEH----VLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEK 1749          

HSP 5 Score: 85.5001 bits (210), Expect = 1.974e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC S+  ++    +  C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +   +        C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC   G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQ-EHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGEKVCTFGAVCSAGQCVCPRCEH 520          

HSP 6 Score: 59.3066 bits (142), Expect = 2.313e-7
Identity = 82/308 (26.62%), Postives = 111/308 (36.04%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE----------------------------EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I D  S D  C C    C      PVCGSD +TY  ECDL +A C  +  L V +   C                              A   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +V     C       P+ C +  C+FGA C E +    C+C ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I      PC    T
Sbjct:  588 IWDEDSEDGPCVCD-FSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPATGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPM-TPVTCVEIHCEFGASCVEKAGFAQCICPTLTC------PEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVT 869          

HSP 7 Score: 56.9954 bits (136), Expect = 1.143e-6
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+   K+G + C C  + C    +  VCGSD +TY NEC L    C +R++++  S  PC+E+
Sbjct:  796 CVEIHCEFGASCV--EKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQES 867          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:Agrn "Agrin" species:10116 "Rattus norvegicus" [GO:0001932 "regulation of protein phosphorylation" evidence=IDA] [GO:0002162 "dystroglycan binding" evidence=IDA] [GO:0005509 "calcium ion binding" evidence=ISS;IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005576 "extracellular region" evidence=TAS] [GO:0005605 "basal lamina" evidence=ISS] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0032321 "positive regulation of Rho GTPase activity" evidence=IDA] [GO:0033691 "sialic acid binding" evidence=ISS] [GO:0035374 "chondroitin sulfate binding" evidence=ISS] [GO:0043113 "receptor clustering" evidence=ISS;IDA] [GO:0043395 "heparan sulfate proteoglycan binding" evidence=ISS] [GO:0045202 "synapse" evidence=IDA] [GO:0045887 "positive regulation of synaptic growth at neuromuscular junction" evidence=ISS] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IDA] [GO:0051290 "protein heterotetramerization" evidence=IDA] [GO:0051491 "positive regulation of filopodium assembly" evidence=IDA] InterPro:IPR000742 InterPro:IPR002350 Pfam:PF00008 Pfam:PF00050 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 RGD:2067 GO:GO:0016021 GO:GO:0005886 GO:GO:0030154 GO:GO:0005509 GO:GO:0030054 GO:GO:0045202 GO:GO:0007528 GO:GO:0045887 GO:GO:0045944 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0007416 GO:GO:0005605 GO:GO:0043113 PROSITE:PS01186 GO:GO:0033691 GO:GO:0002162 CTD:375790 eggNOG:NOG312635 HOGENOM:HOG000033860 HOVERGEN:HBG080471 KO:K06254 GO:GO:0035374 GO:GO:0043395 GO:GO:0051491 GO:GO:0032321 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 EMBL:M64780 EMBL:S44194 PIR:JH0399 RefSeq:NP_786930.1 UniGene:Rn.2163 PDB:3V64 PDB:3V65 PDBsum:3V64 PDBsum:3V65 ProteinModelPortal:P25304 SMR:P25304 IntAct:P25304 STRING:10116.ENSRNOP00000050623 PaxDb:P25304 PRIDE:P25304 GeneID:25592 KEGG:rno:25592 InParanoid:P25304 NextBio:607271 Genevestigator:P25304 GO:GO:0051290 GO:GO:0001932 Uniprot:P25304)

HSP 1 Score: 127.872 bits (320), Expect = 1.944e-28
Identity = 109/385 (28.31%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    F+C C +G  GS CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  + +         + F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL  +L++GG+ P +   M   R + G  +G  GCI+ L IN + + L   +    ++  G+ EC ++ C  NPC    LC +   G FLC C     G +C   ++  C PNPC     C +   G   C C      GT+C     +T   +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1224 CDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCE------KVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDGR---------VQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGI-PEEQVAMVLDRTSVG--VGLKGCIRMLDINNQQLELSDWQR-AAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSP-CQPNPCHGAAPCRVLSSGGAKCECPLGR-SGTFCQ-TVLETAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1567          

HSP 2 Score: 121.324 bits (303), Expect = 2.088e-26
Identity = 119/397 (29.97%), Postives = 171/397 (43.07%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA C V  K+G + C+C+ + CSG I  PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +     + C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +   + G C   +         +   C +  C   G + +EDS    CVC  +C+   + P      VCG DG TY TE DL++  C+ Q+E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CHGVQCAFGAVCTV--KNGKAECECQRV-CSG-IYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDP---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGE-----KVCTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQV--QIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSP------VCGSDGVTYGTECDLKKARCESQQELYVAAQGAC-RGPT-LAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPATG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.9005 bits (237), Expect = 1.367e-18
Identity = 65/233 (27.90%), Postives = 111/233 (47.64%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  L   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I  ++          +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1719 NPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGR-TYIEY---LNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1943          

HSP 4 Score: 95.1301 bits (235), Expect = 2.394e-18
Identity = 90/320 (28.12%), Postives = 137/320 (42.81%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C CP    P     +C   K  S C PNPC G   C     G   C C  G +G+ C+  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L+IGG      P   K+ + A  S GF G I+ + +    +  Q      V+R   +    ++ C+   GNPC N G C+ +   + C C   F+G  C++
Sbjct: 1465 CQCP----PGRFGPTCADEK--SPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA-----PDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEH----VLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEK 1749          

HSP 5 Score: 85.5001 bits (210), Expect = 2.130e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC S+  ++    +  C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +   +        C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC   G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQ-EHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGEKVCTFGAVCSAGQCVCPRCEH 520          

HSP 6 Score: 58.9214 bits (141), Expect = 2.393e-7
Identity = 82/308 (26.62%), Postives = 111/308 (36.04%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE----------------------------EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I D  S D  C C    C      PVCGSD +TY  ECDL +A C  +  L V +   C                              A   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +V     C       P+ C +  C+FGA C E +    C+C ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I      PC    T
Sbjct:  588 IWDEDSEDGPCVCD-FSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPATGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPM-TPVTCVEIHCEFGASCVEKAGFAQCICPTLTC------PEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVT 869          

HSP 7 Score: 56.9954 bits (136), Expect = 1.143e-6
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+   K+G + C C  + C    +  VCGSD +TY NEC L    C +R++++  S  PC+E+
Sbjct:  796 CVEIHCEFGASCV--EKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQES 867          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:Agrn "agrin" species:10116 "Rattus norvegicus" [GO:0001932 "regulation of protein phosphorylation" evidence=IDA] [GO:0001934 "positive regulation of protein phosphorylation" evidence=ISO] [GO:0002162 "dystroglycan binding" evidence=IDA] [GO:0005509 "calcium ion binding" evidence=ISS;IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005576 "extracellular region" evidence=TAS] [GO:0005578 "proteinaceous extracellular matrix" evidence=TAS] [GO:0005604 "basement membrane" evidence=ISO;IDA] [GO:0005605 "basal lamina" evidence=IEA;ISO;ISS] [GO:0005615 "extracellular space" evidence=IEA;ISO] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005886 "plasma membrane" evidence=IEA;ISO] [GO:0007009 "plasma membrane organization" evidence=IEA;ISO] [GO:0007268 "synaptic transmission" evidence=ISO;IMP] [GO:0007416 "synapse assembly" evidence=IMP] [GO:0007528 "neuromuscular junction development" evidence=IEA;ISO;TAS] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IEA;ISO] [GO:0009986 "cell surface" evidence=IEA;ISO] [GO:0010469 "regulation of receptor activity" evidence=ISO] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IEA;ISO] [GO:0031012 "extracellular matrix" evidence=ISO] [GO:0032092 "positive regulation of protein binding" evidence=ISO] [GO:0032314 "regulation of Rac GTPase activity" evidence=ISO] [GO:0032321 "positive regulation of Rho GTPase activity" evidence=IDA] [GO:0033691 "sialic acid binding" evidence=ISS] [GO:0035374 "chondroitin sulfate binding" evidence=ISO;ISS] [GO:0043113 "receptor clustering" evidence=IEA;ISO;ISS;IDA] [GO:0043395 "heparan sulfate proteoglycan binding" evidence=ISO;ISS] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IEA;ISO] [GO:0045202 "synapse" evidence=IEA;ISO;IDA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IEA;ISO] [GO:0045887 "positive regulation of synaptic growth at neuromuscular junction" evidence=ISO;ISS] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA;ISO;IDA] [GO:0051290 "protein heterotetramerization" evidence=IDA] [GO:0051491 "positive regulation of filopodium assembly" evidence=IDA] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] [GO:2000541 "positive regulation of protein geranylgeranylation" evidence=ISO] InterPro:IPR000742 InterPro:IPR002350 Pfam:PF00008 Pfam:PF00050 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 RGD:2067 GO:GO:0016021 GO:GO:0005886 GO:GO:0030154 GO:GO:0005509 GO:GO:0030054 GO:GO:0045202 GO:GO:0007528 GO:GO:0045887 GO:GO:0045944 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0007416 GO:GO:0005605 GO:GO:0043113 PROSITE:PS01186 GO:GO:0033691 GO:GO:0002162 CTD:375790 eggNOG:NOG312635 HOGENOM:HOG000033860 HOVERGEN:HBG080471 KO:K06254 GO:GO:0035374 GO:GO:0043395 GO:GO:0051491 GO:GO:0032321 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 EMBL:M64780 EMBL:S44194 PIR:JH0399 RefSeq:NP_786930.1 UniGene:Rn.2163 PDB:3V64 PDB:3V65 PDBsum:3V64 PDBsum:3V65 ProteinModelPortal:P25304 SMR:P25304 IntAct:P25304 STRING:10116.ENSRNOP00000050623 PaxDb:P25304 PRIDE:P25304 GeneID:25592 KEGG:rno:25592 InParanoid:P25304 NextBio:607271 Genevestigator:P25304 GO:GO:0051290 GO:GO:0001932 Uniprot:P25304)

HSP 1 Score: 127.872 bits (320), Expect = 1.944e-28
Identity = 109/385 (28.31%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    F+C C +G  GS CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  + +         + F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL  +L++GG+ P +   M   R + G  +G  GCI+ L IN + + L   +    ++  G+ EC ++ C  NPC    LC +   G FLC C     G +C   ++  C PNPC     C +   G   C C      GT+C     +T   +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1224 CDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCE------KVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDGR---------VQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGI-PEEQVAMVLDRTSVG--VGLKGCIRMLDINNQQLELSDWQR-AAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSP-CQPNPCHGAAPCRVLSSGGAKCECPLGR-SGTFCQ-TVLETAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1567          

HSP 2 Score: 121.324 bits (303), Expect = 2.088e-26
Identity = 119/397 (29.97%), Postives = 171/397 (43.07%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA C V  K+G + C+C+ + CSG I  PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +     + C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +   + G C   +         +   C +  C   G + +EDS    CVC  +C+   + P      VCG DG TY TE DL++  C+ Q+E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CHGVQCAFGAVCTV--KNGKAECECQRV-CSG-IYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDP---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGE-----KVCTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQV--QIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSP------VCGSDGVTYGTECDLKKARCESQQELYVAAQGAC-RGPT-LAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPATG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.9005 bits (237), Expect = 1.367e-18
Identity = 65/233 (27.90%), Postives = 111/233 (47.64%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  L   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I  ++          +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1719 NPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGR-TYIEY---LNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1943          

HSP 4 Score: 95.1301 bits (235), Expect = 2.394e-18
Identity = 90/320 (28.12%), Postives = 137/320 (42.81%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C CP    P     +C   K  S C PNPC G   C     G   C C  G +G+ C+  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L+IGG      P   K+ + A  S GF G I+ + +    +  Q      V+R   +    ++ C+   GNPC N G C+ +   + C C   F+G  C++
Sbjct: 1465 CQCP----PGRFGPTCADEK--SPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA-----PDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEH----VLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEK 1749          

HSP 5 Score: 85.5001 bits (210), Expect = 2.130e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC S+  ++    +  C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +   +        C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC   G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQ-EHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGEKVCTFGAVCSAGQCVCPRCEH 520          

HSP 6 Score: 58.9214 bits (141), Expect = 2.393e-7
Identity = 82/308 (26.62%), Postives = 111/308 (36.04%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE----------------------------EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I D  S D  C C    C      PVCGSD +TY  ECDL +A C  +  L V +   C                              A   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +V     C       P+ C +  C+FGA C E +    C+C ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I      PC    T
Sbjct:  588 IWDEDSEDGPCVCD-FSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPATGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPM-TPVTCVEIHCEFGASCVEKAGFAQCICPTLTC------PEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVT 869          

HSP 7 Score: 56.9954 bits (136), Expect = 1.143e-6
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+   K+G + C C  + C    +  VCGSD +TY NEC L    C +R++++  S  PC+E+
Sbjct:  796 CVEIHCEFGASCV--EKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQES 867          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:AGRN "Agrin" species:9031 "Gallus gallus" [GO:0005605 "basal lamina" evidence=IEA] [GO:0007213 "G-protein coupled acetylcholine receptor signaling pathway" evidence=IEA] [GO:0043113 "receptor clustering" evidence=IEA] [GO:0043236 "laminin binding" evidence=IEA] InterPro:IPR000742 InterPro:IPR002350 InterPro:IPR004850 Pfam:PF00008 Pfam:PF00050 Pfam:PF03146 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51121 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 InterPro:IPR000152 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 GO:GO:0007213 PROSITE:PS01186 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 InterPro:IPR008993 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF50242 SUPFAM:SSF82671 PROSITE:PS00010 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OrthoDB:EOG7BGHJZ GeneTree:ENSGT00530000063501 EMBL:AADN03007988 Ensembl:ENSGALT00000003171 Uniprot:F1P3E3)

HSP 1 Score: 127.872 bits (320), Expect = 2.145e-28
Identity = 106/380 (27.89%), Postives = 173/380 (45.53%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD +PC  GGTC++    F+C C +G  G+ CE  +        SF G+ S++  K+   +   + I + FR   +  GLLLY       N +N  + F   I L+              +   ++ GSG   + S  ++E GKW  LV  R  + G+L ++    V G +      LNL   L++GG  P D   M  V +    ++G  GCI+ L +N +   L+  +   V+   G+ EC  + C  NPC + G+C + + + F C C   +TG +C   RN  CDP PC  +  C +  + G +C C  G E  G +C+ +  +  +  P       P+ +  + L  +  ++     +  M +EV FL K P  +
Sbjct: 1352 CDSHPCLHGGTCEDDGKEFTCSCPAGKGGAVCEKPIRYF---IPSFGGK-SYLAFKM-MKAYHTVRIAMEFRATELS-GLLLY-------NGQNRGKDF---ISLALVGG---------FVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEPHVSGESPTGTDGLNLDTDLFVGGA-PED--QMAVVAERTAATVGLKGCIRLLDVNNQMYDLRE-KGSDVLYGSGVGECGNDPCHPNPCHHGGICHVKEAEMFHCECLHSYTGPTCADERNP-CDPTPCHISATCLVLPEGGAMCACPMGRE--GEFCE-RVTEQDHTMPFL-----PEFNGFSYLELNGLQTFLTCSKDKMSMEVVFLAKSPSGM 1693          

HSP 2 Score: 108.227 bits (269), Expect = 2.414e-22
Identity = 111/398 (27.89%), Postives = 165/398 (41.46%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDP---VCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE----------EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C+V  K+ +  C+C+ + C GR + PVCGSD+ TY N C+L    C  +  + V    PC+    CG   C FG  C   TG                G +   Y S+C + + AC ++  N+ +++  +C SC  T C +   C+  +      C CP C      E+ P   VCGTD +TYD+ C LRA++C +++   + V   G C           G  S      C           ED  CVC  TC    + P      VCG D  TY  E +L++  C+ ++++ V     C    T      +     C      C  +      G       ++C+    GS         GGTCD   G   C C+ G+ G +C+
Sbjct:  471 CLSVECTFGATCVV--KNREPVCECQQV-CQGRYD-PVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDR---CGK--CQFGAICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRAC-TQQKNILVAAQGDCKSCGSTVCSFGSTCVGGQ------CVCPRC------EQQPLAQVCGTDGLTYDNRCELRAASCQQQK--SIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRSP------VCGSDDVTYANECELKKTRCEKRQDLYVTSQGACRALTTTPPPLPV---VHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSY--------GGTCDPATG--QCSCKPGVGGLKCD 825          

HSP 3 Score: 97.4413 bits (241), Expect = 4.492e-19
Identity = 127/504 (25.20%), Postives = 196/504 (38.89%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVI--------------RKLLETEASS------GSNVEGCRHSNCRFGASC-IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCK-WNEDCLYSEKSGK-SLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG-----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECV-NNRCICPEGTSPNSANTSCEI 524
            FG P  C   +S G  R+FF   V+ + +  +P+    L+ +  L+R+               ++LL  + +S       +  + CR   C FGA C         + C CK   C   +  PVCGSD+ TY NEC+L +A C ++  + VIS  PC     C    C FG +CVR+                       G++ K Y+S+C +   AC+ +  N+       C  C       N  C  + ++ +  L S PE CPS    + +PVCG D +TY S C +              R+  C  +       K+  VC   +      C + +CD  +  VC  DS    +        C ++   P  ++G                                          VCG D RTY    +L   AC +++EI V    PC R G  + G  C  E   C+   +ECV +++ +C  GT  N+  + CE+
Sbjct:   92 FGDPLICDNQVSTGDTRIFF---VNPAPQYMWPAHRNELMLNSSLMRITLRNLEEVEHCVEEHKQLLADKPNSYFTQTPPTPRDACRGMLCGFGAVCERSPTDPSQASCVCKKTACP-VVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTARCVCPASCSGVAESIVCGSDGKDYRSECDLNKHACDKQ-ENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPS----KREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDECKFNAVCLNRRGTARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVKNREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQFGAICEAETGRCVC-PTECVPSSQPVC--GTDGNTYGSECEL 583          

HSP 4 Score: 89.7373 bits (221), Expect = 9.541e-17
Identity = 63/252 (25.00%), Postives = 114/252 (45.24%), Query Frame = 0
Query:  533 PNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH---DLSLTDMNTASFTGQ---DSHVEIKIPTDSV-----------KRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL--QSMREPLVI 765
            PNPCQ GGTC     ++ C C+ G +G+ CE    + +  D    +F G+   + H  +    D++           +    E+  +T   Q  +L  G+ +  S+Y          I L+  +           +   YDLGS  V L+S   I    WTH+ A R  ++G L++     + GS+      L+  G LW+GG++     + +K+ K    S GF+GCI+++ ++++ + L   ++  P ++
Sbjct: 1843 PNPCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSGKGLERSDY----------IALAIVDG---------FVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGME--RLSVAHKLPK--AYSTGFIGCIRDVIVDRQELHLVEDALNNPTIL 2071          

HSP 5 Score: 81.2629 bits (199), Expect = 4.707e-14
Identity = 70/293 (23.89%), Postives = 123/293 (41.98%), Query Frame = 0
Query:  531 CDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEH--DLSLTDMNTASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINK-KTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            CDP PC    TC    +G   C C  G  G  CE   +   T      F G  S++E+       T S  +M +E++F   +    +   GQ  +             F+ L+  +          ++ + YDLG GA  L+S   +    W  ++ +R  + G++ +   ++V+G +  S +     LNL+   ++GG      P   K+ + A  S  F G ++ + I     +  Q +R  + I       C +     NPC+N G C  +++ + C C+R F+G  C++
Sbjct: 1609 CDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGF-SYLELNGLQTFLTCSKDKMSMEVVFLAKSPSGMIFYNGQKTDGKGD---------FVSLALHDG---------YLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGA-----PDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKP---NPCQNGGTCSPRLESYECACQRGFSGAHCEK 1874          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:AGRN "Agrin" species:9031 "Gallus gallus" [GO:0005605 "basal lamina" evidence=IEA] [GO:0005615 "extracellular space" evidence=IEA] [GO:0007009 "plasma membrane organization" evidence=IEA] [GO:0007213 "G-protein coupled acetylcholine receptor signaling pathway" evidence=IEA] [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IEA] [GO:0009986 "cell surface" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IEA] [GO:0043113 "receptor clustering" evidence=IEA] [GO:0043236 "laminin binding" evidence=IEA] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IEA] [GO:0045202 "synapse" evidence=IEA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000742 InterPro:IPR002350 InterPro:IPR004850 Pfam:PF00008 Pfam:PF00050 Pfam:PF03146 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51121 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 InterPro:IPR000152 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 GO:GO:0007213 PROSITE:PS01186 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 InterPro:IPR008993 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF50242 SUPFAM:SSF82671 PROSITE:PS00010 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 TreeFam:TF326548 GeneTree:ENSGT00530000063501 EMBL:AADN03007988 Ensembl:ENSGALT00000040179 Uniprot:F1NWQ6)

HSP 1 Score: 127.872 bits (320), Expect = 2.273e-28
Identity = 109/382 (28.53%), Postives = 170/382 (44.50%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTAD--TPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD +PC  GGTC++    F+C C +G  G+ CE  +        SF G+ S++  K+   +   + I + FR   +  GLLLY       N +N  + F   I L+              +   ++ GSG   + S  ++E GKW  LV  R  + G+L ++    V G +      LNL   L++GG  P D   M  V +    ++G  GCI+ L +N +   L+  +   V+   G+ EC  + C  NPC + G+C + + + F C C   +TG +C   RN  CDP PC  +  C +  + G +C C  G E  G +C+  T    T    P +      + +   T   DL+          M +EV FL K P  +
Sbjct: 1352 CDSHPCLHGGTCEDDGKEFTCSCPAGKGGAVCEKPIRYF---IPSFGGK-SYLAFKM-MKAYHTVRIAMEFRATELS-GLLLY-------NGQNRGKDF---ISLALVGG---------FVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEPHVSGESPTGTDGLNLDTDLFVGGA-PED--QMAVVAERTAATVGLKGCIRLLDVNNQMYDLRE-KGSDVLYGSGVGECGNDPCHPNPCHHGGICHVKEAEMFHCECLHSYTGPTCADERNP-CDPTPCHISATCLVLPEGGAMCACPMGRE--GEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTFVPDLQDK--------MSMEVVFLAKSPSGM 1693          

HSP 2 Score: 108.227 bits (269), Expect = 2.602e-22
Identity = 111/398 (27.89%), Postives = 165/398 (41.46%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDP---VCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE----------EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C+V  K+ +  C+C+ + C GR + PVCGSD+ TY N C+L    C  +  + V    PC+    CG   C FG  C   TG                G +   Y S+C + + AC ++  N+ +++  +C SC  T C +   C+  +      C CP C      E+ P   VCGTD +TYD+ C LRA++C +++   + V   G C           G  S      C           ED  CVC  TC    + P      VCG D  TY  E +L++  C+ ++++ V     C    T      +     C      C  +      G       ++C+    GS         GGTCD   G   C C+ G+ G +C+
Sbjct:  471 CLSVECTFGATCVV--KNREPVCECQQV-CQGRYD-PVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDR---CGK--CQFGAICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRAC-TQQKNILVAAQGDCKSCGSTVCSFGSTCVGGQ------CVCPRC------EQQPLAQVCGTDGLTYDNRCELRAASCQQQK--SIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRSP------VCGSDDVTYANECELKKTRCEKRQDLYVTSQGACRALTTTPPPLPV---VHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSY--------GGTCDPATG--QCSCKPGVGGLKCD 825          

HSP 3 Score: 100.138 bits (248), Expect = 7.239e-20
Identity = 129/504 (25.60%), Postives = 196/504 (38.89%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVI--------------RKLLETEASS------GSNVEGCRHSNCRFGASC-IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCK-WNEDCLYSEKSGK-SLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG-----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECV-NNRCICPEGTSPNSANTSCEI 524
            FG P  C   +S G  R+FF   V+ + +  +P+    L+ +  L+R+               RKLL  + +S       +  + CR   C FGA C         + C CK   C   +  PVCGSD+ TY NEC+L +A C ++  + VIS  PC     C    C FG +CVR+                       G++ K Y+S+C +   AC+ +  N+       C  C       N  C  + ++ +  L S PE CPS    + +PVCG D +TY S C +              R+  C  +       K+  VC   +      C + +CD  +  VC  DS    +        C ++   P  ++G                                          VCG D RTY    +L   AC +++EI V    PC R G  + G  C  E   C+   +ECV +++ +C  GT  N+  + CE+
Sbjct:   92 FGDPLICDNQVSTGDTRIFF---VNPAPQYMWPAHRNELMLNSSLMRITLRNLEEVEHCVEEHRKLLADKPNSYFTQTPPTPRDACRGMLCGFGAVCERSPTDPSQASCVCKKTACP-VVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTARCVCPASCSGVAESIVCGSDGKDYRSECDLNKHACDKQ-ENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPS----KREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDECKFNAVCLNRRGTARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVKNREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQFGAICEAETGRCVC-PTECVPSSQPVC--GTDGNTYGSECEL 583          

HSP 4 Score: 95.5153 bits (236), Expect = 1.670e-18
Identity = 62/241 (25.73%), Postives = 113/241 (46.89%), Query Frame = 0
Query:  533 PNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH---DLSLTDMNTASFTGQ---DSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL--QSMREPLVI 765
            PNPCQ GGTC     ++ C C+ G +G+ CE    + +  D    +F G+   + H  +     +++    E+  +T   Q  +L  G+ +  S+Y          I L+  +           +   YDLGS  V L+S   I    WTH+ A R  ++G L++     + GS+      L+  G LW+GG++     + +K+ K    S GF+GCI+++ ++++ + L   ++  P ++
Sbjct: 1843 PNPCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSEKALQSNHFELSIKTEATQGLILWSGKGLERSDY----------IALAIVDG---------FVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGME--RLSVAHKLPK--AYSTGFIGCIRDVIVDRQELHLVEDALNNPTIL 2060          

HSP 5 Score: 82.8037 bits (203), Expect = 1.413e-14
Identity = 69/294 (23.47%), Postives = 126/294 (42.86%), Query Frame = 0
Query:  531 CDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCE------HDLS-LTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINK-KTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            CDP PC    TC    +G   C C  G  G  CE      H +  L + N  S+  + + ++  +P D   +M +E++F   +    +   GQ  +             F+ L+  +          ++ + YDLG GA  L+S   +    W  ++ +R  + G++ +   ++V+G +  S +     LNL+   ++GG      P   K+ + A  S  F G ++ + I     +  Q +R  + I       C +     NPC+N G C  +++ + C C+R F+G  C++
Sbjct: 1609 CDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYL-ELNGLQTFVP-DLQDKMSMEVVFLAKSPSGMIFYNGQKTDGKGD---------FVSLALHDG---------YLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGA-----PDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKP---NPCQNGGTCSPRLESYECACQRGFSGAHCEK 1874          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:AGRN "Uncharacterized protein" species:9913 "Bos taurus" [GO:0005605 "basal lamina" evidence=IEA] [GO:0005615 "extracellular space" evidence=IEA] [GO:0007009 "plasma membrane organization" evidence=IEA] [GO:0007213 "G-protein coupled acetylcholine receptor signaling pathway" evidence=IEA] [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IEA] [GO:0009986 "cell surface" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IEA] [GO:0043113 "receptor clustering" evidence=IEA] [GO:0043236 "laminin binding" evidence=IEA] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IEA] [GO:0045202 "synapse" evidence=IEA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000742 InterPro:IPR002350 InterPro:IPR004850 Pfam:PF00008 Pfam:PF00050 Pfam:PF03146 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51121 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 GO:GO:0005615 GO:GO:0009986 GO:GO:0043525 GO:GO:0045202 GO:GO:0007528 GO:GO:0045944 GO:GO:0007009 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 GO:GO:0007213 PROSITE:PS01186 GO:GO:0030548 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 InterPro:IPR008993 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF50242 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OMA:PRCSCDR OrthoDB:EOG7BGHJZ TreeFam:TF326548 GO:GO:0045213 GO:GO:0010469 GeneTree:ENSGT00530000063501 GO:GO:0008582 InterPro:IPR009030 SUPFAM:SSF57184 EMBL:DAAA02043249 Ensembl:ENSBTAT00000017563 Uniprot:F1MSI2)

HSP 1 Score: 123.25 bits (308), Expect = 5.018e-27
Identity = 108/383 (28.20%), Postives = 161/383 (42.04%), Query Frame = 0
Query:  531 CDPNPCQGGGTC-DEHDGT-FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD  PC  GGTC D+  G  F+C C +G  G+ CE  L     +  +F G   H  +  PT  +   + + + FR    Q GLLLY  +    ++   +                        + F +D GSG   L S   ++ G+W HL   R+ + G L ++    V+G +      LNL   L++GGV P D      V +    SIG  GCI+ L +N + + L S  E    R  G+ +C ++ C  +PC     C +   G F C C     G +C   + D C PNPC     C +  +G   C C  G E  G+ C  +T   P D   + +  +  ++         ++ LG      M LEV FL + P  L
Sbjct: 1320 CDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKAL---HPSVPAFGG---HSFLAFPTLRAYHTLRLALEFRALEPQ-GLLLYNGNARGKDFLGLVLLG-------------------GRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGV-PED--QASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPE-SATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEK-DPCQPNPCHGAAPCRVLPQGEAKCECPHGRE--GSLC--QTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLG----EKMALEVVFLARSPSGL 1663          

HSP 2 Score: 118.242 bits (295), Expect = 2.196e-25
Identity = 118/398 (29.65%), Postives = 166/398 (41.71%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            CR   C FGA C V  K+G++ C C    CSG +  PVCGSD ITY + C+L    C     + V    PC+    CG   C FG  C   TG                G++ + Y S+C + + AC  +++ L ++S   C +C  T      C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +   + G C   +         +   C    C   G + +EDS    CVC  +C+   + P      VCG DG TY TE +L++  C+ Q E+ V     C RG T +          C      C  +     +G       +SC+    GS         GGTCD   G   C CR G+ G +C+ 
Sbjct:  459 CRGVQCPFGAMCAV--KNGEAECACPKA-CSG-VYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDR---CGQ--CRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQIS-LHVASAGPCQTCGDTV-----CAFGAVCSAGQCVCPRCERPPPG-----PVCGSDGVTYGSSCELREAACQQQ--TQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSP------VCGSDGVTYRTECELKKARCESQPELYVVAQGAC-RGPT-LAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSY--------GGTCDPVTG--QCSCRPGVGGLKCDR 816          

HSP 3 Score: 105.531 bits (262), Expect = 1.678e-21
Identity = 95/314 (30.25%), Postives = 137/314 (43.63%), Query Frame = 0
Query:  202 CRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSC-VRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC 495
            CRF A C+  ++ G   C C  + C G   +PVC  D  TY N+C   QA C ++ ++ +    PC+++   C    CPFG  C V+ G                  G++   Y S C +E  AC +    + ++    C  C Q C++   C    ++G+ +C     PS       PVCG+D  TY S C L    C+ +    + V   G C+T        CG + C FGAVC     CVC    + E   P    G VCG DG TY +  +LR  AC+ Q +I  A   PC +     G     E+ +C
Sbjct:  390 CRFNAVCL--SRRGRPRCSCDRVVCDGAY-RPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATAC-ALGREIRVARRGPCDRCGQ-CRFGALC--EAETGRCVC-----PSECVASAQPVCGSDGRTYASECELHVHACTHQ--ISLHVASAGPCQT--------CGDTVCAFGAVCSA-GQCVCP---RCERPPP----GPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGEC 673          

HSP 4 Score: 102.834 bits (255), Expect = 1.032e-20
Identity = 87/307 (28.34%), Postives = 128/307 (41.69%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGG--------------------NNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNE---DCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE-EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSP 515
            PVCG D +TY N+C + + G  + I L  +    C+    C  + C F   C+   G                    ++   Y + C  +   C+ +  ++ M     C      C+  +     + + K+G++ C+CP+   A +   DPVCG+D ITY S C L A+ C+   G ++RV   G C          CG+  C FGA+CE E   CVC   C    Q        VCG DGRTY +E +L  HAC  Q  + VA   PC      +            A  + C   +C+CP    P
Sbjct:  346 PVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQC-PEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQ-RSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPK---ACSGVYDPVCGSDGITYGSACELEATACAL--GREIRVARRGPCDR--------CGQ--CRFGALCEAETGRCVCPSECVASAQ-------PVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTV-----------CAFGAVCSAGQCVCPRCERP 617          

HSP 5 Score: 90.5077 bits (223), Expect = 6.822e-17
Identity = 120/480 (25.00%), Postives = 177/480 (36.88%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVIRKLLE-----TEASSGSNV--------EGCRHSNCRFGASCIVDA-KSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCV------CSVTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN 505
            FG P  C   +S G  R+FF+      L   +P+    L+ +  L+R+  + LE      E   G++         + CR   C FGA C   A + G + C CK   C   +  PVCGSD  TY NEC+L +A C+++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC +   N+       C  C       S+TC+ N      E     L     CP  G     PVCG D +TYD+ C +              R+  C  +       ++  VC + +      C +  CD  +  VC  D +            C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC R G  R G  C  E   C+   SECV +
Sbjct:   88 FGDPLICDNQVSTGDTRIFFVNPAPPYL---WPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPS-VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHAC-AHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEM----LLRPESCPPRGT----PVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVC-PSECVAS 553          

HSP 6 Score: 88.5817 bits (218), Expect = 2.124e-16
Identity = 59/229 (25.76%), Postives = 106/229 (46.29%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRME---IEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
             PC  G +C   + ++ C C +G +G  CE  L   S  D++  +F G+     +   T+S K ++    E+  RT   Q  +L  G++   ++Y          I L+  + +         +  +YDLGS  V L+S   +   +W  + A R  ++G L++     V GS+      L+  G LW+GG++    P    + K  GT  GFVGC++++ + ++ + L
Sbjct: 1811 QPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADY----------IALAIVDGR---------LQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLE--KLPTGQALPKAYGT--GFVGCLRDVVVGQRPVHL 2016          

HSP 7 Score: 86.6557 bits (213), Expect = 1.036e-15
Identity = 77/292 (26.37%), Postives = 125/292 (42.81%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C PNPC G   C     G   C C  G  GS C+      D     A F+   S++E+K       D  ++M +E++F   +   GLLLY GQ  +             F+ L+  N           + F YDLG GA  ++S   +  G WT +  +R  + G + +    +V+G +      LNL+  L++GG      P   K+ + A  S GF G I+ + +N +    Q +    V+R   +    ++ C+   G PC +   C+ +   + C C   F+G  C++
Sbjct: 1579 CQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFS-SFSYLELKGLHTFERDLGEKMALEVVFLARS-PSGLLLYNGQKTDGKGD---------FVSLALHNGL---------LEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLFVGGA-----PDFSKLARAAAVSSGFDGAIQLVSLNGR----QLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEK 1841          

HSP 8 Score: 81.2629 bits (199), Expect = 4.128e-14
Identity = 90/308 (29.22%), Postives = 118/308 (38.31%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLC----------------------------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVCSV-TCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I D  S D  C C G  C G +  PVCGSD +TY+ EC+L +A C  +  L V++   C    L                             G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G+S C+   C   G   +D     + +T   +CS +      K G   D R   P  C T     P  C +  C+FGA C E++   +CVC   TC      P   A  VCG DG TY  E  LR  AC+   EI +  F PC  G T
Sbjct:  684 IWDEDSEDGPCVC-GFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQC-----NPHGSYGGTCDPVTGQCSCR-PGVGGLKCDRC----EPGFWNFRGIVTD--GRSGCTPCSCDPRGAVRDD----CEQMT--GLCSCKPGVAGPKCGQCPDGRALGPAGCET-DSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTC------PGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGIT 965          

HSP 9 Score: 56.9954 bits (136), Expect = 1.111e-6
Identity = 30/80 (37.50%), Postives = 42/80 (52.50%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSK 276
            C    C FGASC+ +A  G ++C C    C G     VCGSD +TY NEC L    C + + +++ S  PC+E    G +
Sbjct:  892 CAEMLCEFGASCVEEA--GSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPR 969          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:HSPG2 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0001958 "endochondral ossification" evidence=IEA] [GO:0002062 "chondrocyte differentiation" evidence=IEA] [GO:0005605 "basal lamina" evidence=IEA] [GO:0005615 "extracellular space" evidence=IEA] [GO:0007420 "brain development" evidence=IEA] [GO:0008022 "protein C-terminus binding" evidence=IEA] [GO:0008104 "protein localization" evidence=IEA] [GO:0030198 "extracellular matrix organization" evidence=IEA] [GO:0048704 "embryonic skeletal system morphogenesis" evidence=IEA] [GO:0048738 "cardiac muscle tissue development" evidence=IEA] [GO:0060351 "cartilage development involved in endochondral bone morphogenesis" evidence=IEA] [GO:0070062 "extracellular vesicular exosome" evidence=IEA] InterPro:IPR000742 InterPro:IPR002172 InterPro:IPR007110 Pfam:PF00008 Pfam:PF00057 PRINTS:PR00261 PROSITE:PS50026 PROSITE:PS50068 PROSITE:PS50835 SMART:SM00181 SMART:SM00192 GO:GO:0070062 GO:GO:0005615 GO:GO:0008104 GO:GO:0007420 Gene3D:2.60.40.10 InterPro:IPR013783 InterPro:IPR003598 SMART:SM00408 GO:GO:0030198 InterPro:IPR013032 PROSITE:PS00022 GO:GO:0048738 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 PROSITE:PS01186 Gene3D:4.10.400.10 InterPro:IPR023415 SUPFAM:SSF57424 PROSITE:PS01209 InterPro:IPR002049 InterPro:IPR001791 InterPro:IPR000082 Pfam:PF00053 Pfam:PF00054 SMART:SM00180 SMART:SM00282 SMART:SM00200 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OrthoDB:EOG7BGHJZ TreeFam:TF326548 GeneTree:ENSGT00530000063501 InterPro:IPR013098 Pfam:PF07679 GO:GO:0048704 GO:GO:0002062 GO:GO:0001958 Pfam:PF02210 GO:GO:0060351 Pfam:PF12661 InterPro:IPR018031 InterPro:IPR000034 Pfam:PF00052 SMART:SM00281 PROSITE:PS51115 OMA:RQCTSSS EMBL:DAAA02006470 EMBL:DAAA02006471 IntAct:F1MER7 Ensembl:ENSBTAT00000022744 Uniprot:F1MER7)

HSP 1 Score: 119.398 bits (298), Expect = 1.155e-25
Identity = 94/347 (27.09%), Postives = 163/347 (46.97%), Query Frame = 0
Query:  499 NSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTC-DEHDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY--GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENS-CSGNPCKNEGLCISQVQ-GFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            +SE  +  C+CP G + +    S  +      C P  C    TC +  DG  ++C C  G +G RCE  +++T   T S +G DS++ +   T++   + +++ F+     +G+LL+  G+S  V +          F+ L+             H+ F Y+LGSG   L+S   +  G+W H+ A+R ++DG L++     V+ S+ G  + LNL   L++GGV+P       ++      S  F GC+ E+ +N K + L        +  +G+ +C ++S C   PC++   C+   +  F C C+  F G  C+   N      PC + G C    +G  C+C
Sbjct: 3844 DSESSSYVCVCPAGFTGSRCEHSQALH-----CHPEACGPDATCVNRPDGHGYTCRCHLGRSGMRCEEGVTVT---TPSLSGTDSYLALPALTNTHHELRLDVEFKPL-APDGVLLFSGGKSGPVED----------FVSLAMVGG---------HLEFRYELGSGLAVLRSAEPLALGRWHHVSAERLNKDGSLQVNGGRPVLRSSPGKSQGLNLHTLLYLGGVEP-----AVQLPPATNVSAHFHGCVGEVSVNGKRLDLTYS----FLGSQGVGQCYDSSPCERQPCRHGATCMPAGEYEFQCLCRDGFKGDLCEHEENPCQLHEPCLHGGTC----QGTHCLC 4149          

HSP 2 Score: 88.9669 bits (219), Expect = 2.097e-16
Identity = 76/299 (25.42%), Postives = 126/299 (42.14%), Query Frame = 0
Query:  547 GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT--DSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCI-SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVC--HIKDRGFVCVC 839
            GT+ C   +     +    L +       +  Q  +  + +PT  D+ ++ EI+I FR  +  +G+LLY GQ     +  N     P FI       +           F +D GSG  T++    +  G++  +   R    G L +     V G+++G  + L+L   L++GG  P+     Y     AG S GF+GC++EL+I  + I    +     +   G+  C   +C   PC+N G C  S+   ++C C   FTG  C+  +   C P  C  +  C       G+ C C
Sbjct: 3619 GTYVCTATNRQGKVKAFAQLQVPAERVVPYFTQTPYSFLPLPTIKDAYRKFEIKITFRP-DSADGMLLYNGQKQIPGSPANLANRQPDFISFGLVGGRPE---------FRFDAGSGMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGSLAPVNGTSQGKFQGLDLNEELYLGGY-PD-----YGAIPKAGLSSGFIGCVRELRIQGEEIVFHDLN----LTAHGISHCP--TCRDRPCQNGGQCQDSESSSYVCVCPAGFTGSRCEHSQALHCHPEACGPDATCVNRPDGHGYTCRC 3895          

HSP 3 Score: 64.6994 bits (156), Expect = 5.396e-9
Identity = 80/303 (26.40%), Postives = 112/303 (36.96%), Query Frame = 0
Query:  473 DFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCD--PNPCQ------GGGTCDEHDGTFSCYCRSGLTGSRCEHD----LSLTDMNTASFTGQDSHVE----------IKIPTDSVKRM------EIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKEL 747
            D  PC R   R G  C+                +C+C +G               G LC+   NPCQ       GGTC    GT  C C  G +G RC+H     L  +D +     G D+  +          + +P     R        IE+  RT      L+  G+    S           FI L   +          H++FSY LGSG   L S   I  G+W  + A R  Q G ++++  + V G + G    +N +G +++GG  PN       V      S G  GCIK L
Sbjct: 4087 DSSPCERQPCRHGATCMPAGE---------YEFQCLCRDGFK-------------GDLCEHEENPCQLHEPCLHGGTCQ---GT-HCLCPPGFSGPRCQHSSAHGLVESDWHLEGSGGNDAPGQYGAYFYDNGFLALPGRIFSRSLPEVPETIELEVRTSTASGLLVWQGEETGQSGRGKD------FISLGLQDG---------HLVFSYQLGSGEARLVSEDPINDGEWHRVTALREGQRGSIQVDGEELVSGQSPGPNVAVNTKGSIYVGGA-PN-----VAVLTGGRFSSGITGCIKNL 4342          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:AGRN "Agrin" species:9031 "Gallus gallus" [GO:0002162 "dystroglycan binding" evidence=IDA] [GO:0005509 "calcium ion binding" evidence=IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005576 "extracellular region" evidence=TAS] [GO:0005605 "basal lamina" evidence=ISS] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0007171 "activation of transmembrane receptor protein tyrosine kinase activity" evidence=IDA] [GO:0007213 "G-protein coupled acetylcholine receptor signaling pathway" evidence=IEA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0008201 "heparin binding" evidence=IDA] [GO:0010469 "regulation of receptor activity" evidence=IDA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0030297 "transmembrane receptor protein tyrosine kinase activator activity" evidence=IDA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IDA] [GO:0032321 "positive regulation of Rho GTPase activity" evidence=ISS] [GO:0033691 "sialic acid binding" evidence=IDA] [GO:0035374 "chondroitin sulfate binding" evidence=IDA] [GO:0043113 "receptor clustering" evidence=ISS;IDA] [GO:0043236 "laminin binding" evidence=IDA] [GO:0043395 "heparan sulfate proteoglycan binding" evidence=IDA] [GO:0045202 "synapse" evidence=ISS] [GO:0045887 "positive regulation of synaptic growth at neuromuscular junction" evidence=ISS] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=ISS] [GO:0051491 "positive regulation of filopodium assembly" evidence=ISS] InterPro:IPR000742 InterPro:IPR002350 InterPro:IPR004850 Pfam:PF00008 Pfam:PF00050 Pfam:PF03146 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51121 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 InterPro:IPR000152 GO:GO:0016021 GO:GO:0007275 GO:GO:0005886 GO:GO:0030154 GO:GO:0005509 GO:GO:0030054 GO:GO:0045202 GO:GO:0045887 GO:GO:0045944 GO:GO:0008201 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043236 GO:GO:0043113 GO:GO:0007213 PROSITE:PS01186 GO:GO:0033691 GO:GO:0002162 EMBL:U35613 EMBL:M94271 EMBL:M97371 EMBL:M97372 EMBL:U07271 PIR:JH0591 RefSeq:NP_990858.1 UniGene:Gga.4448 UniGene:Gga.48948 PDB:1JB3 PDB:1JC7 PDB:1PXU PDB:1PZ7 PDB:1PZ8 PDB:1PZ9 PDB:1Q56 PDB:3I70 PDBsum:1JB3 PDBsum:1JC7 PDBsum:1PXU PDBsum:1PZ7 PDBsum:1PZ8 PDBsum:1PZ9 PDBsum:1Q56 PDBsum:3I70 ProteinModelPortal:P31696 BioGrid:676782 IntAct:P31696 PaxDb:P31696 PRIDE:P31696 GeneID:396538 KEGG:gga:396538 CTD:375790 eggNOG:NOG312635 HOGENOM:HOG000033860 HOVERGEN:HBG080471 KO:K06254 Reactome:REACT_196873 EvolutionaryTrace:P31696 NextBio:20816574 PRO:PR:P31696 GO:GO:0030548 GO:GO:0035374 GO:GO:0043395 GO:GO:0030297 GO:GO:0051491 GO:GO:0032321 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 InterPro:IPR008993 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF50242 SUPFAM:SSF82671 PROSITE:PS00010 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 Uniprot:P31696)

HSP 1 Score: 118.242 bits (295), Expect = 1.899e-25
Identity = 107/380 (28.16%), Postives = 173/380 (45.53%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD +PC  GGTC++    F+C C +G  G+ CE  +        SF G+ S++  K+   +   + I + FR   +  GLLLY       N +N  +    FI L+              +   ++ GSG   + S  ++E GKW  LV  R  + G+L ++  + V G +      LNL   L++GG  P D   M  V +    ++G  G I+ L +N +   L+  +   V+   G+ EC  + C  NPC +   C + + + F C C   +TG +C   RN  CDP PC  +  C +  + G +C C  G E  G +C+ +  +  +  P       P+ +  + L  +  ++L LT    M +EV FL K P  +
Sbjct: 1351 CDSHPCLHGGTCEDDGREFTCRCPAGKGGAVCEKPIRYF---IPSFGGK-SYLAFKM-MKAYHTVRIAMEFRATELS-GLLLY-------NGQNRGKD---FISLALVGG---------FVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVD-GEHVSGESPTGTDGLNLDTDLFVGGA-PED--QMAVVAERTAATVGLKGSIRLLDVNNQMYDLRE-KGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNP-CDPTPCHISATCLVLPEGGAMCACPMGRE--GEFCE-RVTEQDHTMPFL-----PEFNGFSYLELNGLQTLFLTCRQ-MSMEVVFLAKSPSGM 1690          

HSP 2 Score: 107.457 bits (267), Expect = 4.529e-22
Identity = 111/398 (27.89%), Postives = 164/398 (41.21%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDP---VCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE----------EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C+V  K+ +  C+C+ + C GR + PVCGSD+ TY N C+L    C  +  + V    PC+    CG   C FG  C   TG                G +   Y S+C + + AC ++  N+ +++  +C SC  T C +   C+  +      C CP C      E+ P   VCGTD +TYD+ C LRA++C +++   + V   G C           G  S      C           ED  CVC  TC    + P      VCG D  TY  E +L++  C+ ++ + V     C    T      +     C      C  +      G       ++C+    GS         GGTCD   G   C C+ G+ G +C+
Sbjct:  470 CLSVECTFGATCVV--KNREPVCECQQV-CQGRYD-PVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDR---CGK--CQFGAICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRAC-TQQKNILVAAQGDCKSCGTTVCSFGSTCVGGQ------CVCPRC------EQQPLAQVCGTDGLTYDNRCELRAASCQQQK--SIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRSP------VCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTTTPPPLPV---VHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSY--------GGTCDPATG--QCSCKPGVGGLKCD 824          

HSP 3 Score: 99.7525 bits (247), Expect = 9.355e-20
Identity = 88/306 (28.76%), Postives = 125/306 (40.85%), Query Frame = 0
Query:  232 KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCV--------------------------RTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE-EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICP 510
            +PVCG D +TY +EC + + G  + + +  +    C+    C  + C F   C+                          RT  N+ +  K++CH +            + +   C S    C +   C+   K+ + +C   EC        DPVCG+DN TY + C L A  C  KR  ++RVK+ G C          CGK  C FGA+CE E   CVC   C    Q        VCG DG TY +E +L   AC  QK I+VA    C    T +            +  S CV  +C+CP
Sbjct:  356 EPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDE-CKFNAVCLKRWHARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVE--CTFGATCVV--KNREPVC---ECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKR--EIRVKHKGPCDR--------CGK--CQFGAICEAETGRCVCPTECVPSSQ-------PVCGTDGNTYGSECELHVRACTQQKNILVAAQGDCKSCGTTV-----------CSFGSTCVGGQCVCP 623          

HSP 4 Score: 98.9821 bits (245), Expect = 1.442e-19
Identity = 129/504 (25.60%), Postives = 197/504 (39.09%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVI--------------RKLLETEASS------GSNVEGCRHSNCRFGASC-IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCK-WNEDCLYSEKSGK-SLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG-----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECV-NNRCICPEGTSPNSANTSCEI 524
            FG P  C   +S G  R+FF   V+ + +  +P+    L+ +  L+R+               RKLL  + +S       +  + CR   C FGA C         + C CK   C   +  PVCGSD+ TY NEC+L +A C ++  + VIS  PC     C    C FG +CVR+                       G++ K Y+S+C +   AC+ +  N+       C  C       N  C  + ++ +  L S PE CPS    + +PVCG D +TY S C +              R+  C  +       K+  VC   ++     C + +CD  +  VC  DS    +        C ++   P  ++G                                          VCG D RTY    +L   AC +++EI V    PC R G  + G  C  E   C+   +ECV +++ +C  GT  N+  + CE+
Sbjct:   92 FGDPLICDNQVSTGDTRIFF---VNPAPQYMWPAHRNELMLNSSLMRITLRNLEEVEHCVEEHRKLLADKPNSYFTQTPPTPRDACRGMLCGFGAVCERSPTDPSQASCVCKKTACP-VVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTAGCVCPASCSGVAESIVCGSDGKDYRSECDLNKHACDKQ-ENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPS----KREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDECKFNAVCLK-RWHARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVKNREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQFGAICEAETGRCVC-PTECVPSSQPVC--GTDGNTYGSECEL 582          

HSP 5 Score: 91.6633 bits (226), Expect = 2.632e-17
Identity = 65/260 (25.00%), Postives = 116/260 (44.62%), Query Frame = 0
Query:  533 PNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQ----------DSHVEIKIPT------------DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL--QSMREPLVI 765
            PNPCQ GGTC     ++ C C+ G +G+ CE  +   +  D    +F G+           SH+  +IP              +++    E+  +T   Q  +L  G+ +  S+Y          I L+  +           +   YDLGS  V L+S   I    WTH+ A R  ++G L++     + GS+      L+  G LW+GG++     + +K+ K    S GF+GCI+++ ++++ + L   ++  P ++
Sbjct: 1840 PNPCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSGKGLERSDY----------IALAIVDG---------FVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGME--RLSVAHKLPK--AYSTGFIGCIRDVIVDRQELHLVEDALNNPTIL 2076          

HSP 6 Score: 81.2629 bits (199), Expect = 3.876e-14
Identity = 67/294 (22.79%), Postives = 124/294 (42.18%), Query Frame = 0
Query:  531 CDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCE------HDLS-LTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINK-KTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            CDP PC    TC    +G   C C  G  G  CE      H +  L + N  S+   +    + +   + ++M +E++F   +    +   GQ  +             F+ L+  +          ++ + YDLG GA  L+S   +    W  ++ +R  + G++ +   ++V+G +  S +     LNL+   ++GG      P   K+ + A  S  F G ++ + I     +  Q +R  + I       C +     NPC+N G C  +++ + C C+R F+G  C++
Sbjct: 1607 CDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFL---TCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGD---------FVSLALHDG---------YLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGA-----PDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKP---NPCQNGGTCSPRLESYECACQRGFSGAHCEK 1871          
BLAST of EMLSAG00000004167 vs. GO
Match: - (symbol:AGRN "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0005605 "basal lamina" evidence=IEA] [GO:0005615 "extracellular space" evidence=IEA] [GO:0007009 "plasma membrane organization" evidence=IEA] [GO:0007213 "G-protein coupled acetylcholine receptor signaling pathway" evidence=IEA] [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0008582 "regulation of synaptic growth at neuromuscular junction" evidence=IEA] [GO:0009986 "cell surface" evidence=IEA] [GO:0030548 "acetylcholine receptor regulator activity" evidence=IEA] [GO:0043113 "receptor clustering" evidence=IEA] [GO:0043236 "laminin binding" evidence=IEA] [GO:0043525 "positive regulation of neuron apoptotic process" evidence=IEA] [GO:0045202 "synapse" evidence=IEA] [GO:0045213 "neurotransmitter receptor metabolic process" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000742 InterPro:IPR002350 InterPro:IPR004850 Pfam:PF00008 Pfam:PF00050 Pfam:PF03146 Pfam:PF07648 PROSITE:PS50026 PROSITE:PS51121 PROSITE:PS51465 SMART:SM00181 SMART:SM00280 GO:GO:0005615 GO:GO:0009986 GO:GO:0043525 GO:GO:0045202 GO:GO:0007528 GO:GO:0045944 GO:GO:0007009 InterPro:IPR013032 PROSITE:PS00022 Gene3D:2.60.120.200 InterPro:IPR008985 InterPro:IPR013320 SUPFAM:SSF49899 GO:GO:0005605 GO:GO:0043113 GO:GO:0007213 PROSITE:PS01186 CTD:375790 KO:K06254 GO:GO:0030548 Gene3D:3.30.70.960 InterPro:IPR002049 InterPro:IPR003645 InterPro:IPR001791 InterPro:IPR000082 InterPro:IPR008993 Pfam:PF00053 Pfam:PF00054 Pfam:PF01390 SMART:SM00180 SMART:SM00274 SMART:SM00282 SMART:SM00200 SUPFAM:SSF50242 SUPFAM:SSF82671 PROSITE:PS01248 PROSITE:PS50027 PROSITE:PS50025 PROSITE:PS50024 OMA:PRCSCDR OrthoDB:EOG7BGHJZ TreeFam:TF326548 GO:GO:0045213 GO:GO:0010469 GeneTree:ENSGT00530000063501 GO:GO:0008582 EMBL:AAEX03003837 EMBL:AAEX03003838 RefSeq:XP_536713.3 Ensembl:ENSCAFT00000030741 GeneID:479574 KEGG:cfa:479574 Uniprot:F1Q2Z6)

HSP 1 Score: 118.242 bits (295), Expect = 1.971e-25
Identity = 119/409 (29.10%), Postives = 169/409 (41.32%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSC-------------------DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C V  K+G++ C C+ + CSG I  PVCGSD +TY + C+L    C     + V    PC+    CG   C FG  C   TG                G++   Y S+C + + AC  +++ L + S  +C +C  T C +   CL  +      C CP C  P  G     PVCG+D +TYDS C LR + C ++               I+  +  PC ++ C                     G V +EDS    CVC  +C    + P      VCG DG TY TE +L++  C+ ++E+ V     C RG T +          C      C  +      G       +SC+    GS          GTCD   G   C CR G+ G +C+
Sbjct:  470 CLGVRCPFGATCAV--KNGEAECVCQQV-CSG-IYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDR---CGQ--CRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQIS-LHVVSAGHCQTCGDTVCAFGAVCLAGQ------CVCPRCERPPPG-----PVCGSDGVTYDSACHLREAACQQQ-------------TQIEEARAGPCEQAECGSGGSGSGEDGECEQELCQQHGGVWDEDSEDGPCVCDFSCHSVLRSP------VCGSDGLTYGTECELKKARCESRRELYVTAQGAC-RGPT-LAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQCNPHGSY--------SGTCDPTTG--QCSCRPGVGGLKCD 826          

HSP 2 Score: 114.775 bits (286), Expect = 2.549e-24
Identity = 110/385 (28.57%), Postives = 159/385 (41.30%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHD--GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALME---LEVSFLKKDPGSL 907
            CD  PC  GGTC + D  G F+C C  G  G+ CE  L L+     +F G+     +  PT  +   + + + FR    Q GLLLY  +    +          F+ L+    +         +   +D GSG   L S   IE G+W  L   R+ + G L ++    V+G +      LNL   L++GG+ P D      V +    S+G  GCI+ L +N + + L S       R  G+ EC ++ C  NPC +   C +   G F C C     G +C   +N  C+PNPC     C +   G   C C      G  C    ++  + QP           F+     +L K L   E  L E   LEV FL + P  L
Sbjct: 1334 CDSQPCLHGGTCQDQDSGGGFTCSCPVGRRGAVCEKALYLS---VPAFNGRSF---LAFPTLRAYHTLRLALEFRALEPQ-GLLLYNGNAQGKD----------FLALTLLGGR---------VQLRFDTGSGPAVLTSSVPIEPGRWHRLELSRHWRRGTLSVDGEPLVLGHSPSGTDGLNLDTDLFVGGI-PED--QASVVLERTSISVGLKGCIRLLDVNNQRLELSSWPG-AAARSSGVGECGDHPCLPNPCLDGAPCQALEAGMFHCQCPPGRFGPTCSGDKNP-CEPNPCHGAAPCRVLPDGEAKCECPRGR-GGAICQ-TVSERDSSQPFLAD-------FHGFSYLEL-KGLHTFERDLGEKLALEVVFLARGPSGL 1677          

HSP 3 Score: 97.8265 bits (242), Expect = 3.187e-19
Identity = 122/480 (25.42%), Postives = 181/480 (37.71%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVIRKLLE-----TEASSGSNVEG--------CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVC--------------SLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCV------CSVTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN 505
            FG P  C   +S G  R+FF+      L   +P+    L+ +  L+R+  + LE      E   G++           CR   C FGA C   A  SG + C CK   C   +  PVCGSD  TY NEC+L +A C+++  + ++S  PC     C +  C FG +CVR+                       G++   Y S+C +   AC +R  N+       C  C       S+ C+ +    + E   +   SCP  P+       PVCG D +TYD+ C               +R+  C  +       ++  VC + +      C +  CD  +  VC  D +            C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC R G  R G  C  E   C+   SECV +
Sbjct:   99 FGDPLICDNQVSTGDTRIFFVNPAPPYL---WPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPAPPTPPDACRGMLCGFGAVCEPSAGGSGRASCVCKKSACPA-VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCVRSADGQTATCLCPATCRGPPEGPVCGSDGADYPSECQLLRLAC-TRQENIVKKFDGPCDPCQGTHHDLSRVCRVHPRTRHPEMLLRPE-SCPSQPA-------PVCGDDGVTYDNDCIMGRTGATRGLVLQKVRSGQCQPQDQCPDVCRFNAVCLSRRGRPRCSCDRVICDGAYRPVCAHDGHTYDNDCWRQQAECRQQRTIPAKHQGPCDQPLSPCLGVRCPFGATCAVKNGEAECVCQQVCSGIYDPVCGSDGVTYGSTCELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVC-PSECVAS 564          

HSP 4 Score: 89.3521 bits (220), Expect = 1.290e-16
Identity = 59/229 (25.76%), Postives = 107/229 (46.72%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRME---IEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            +PC  G +C   + ++ C C  G +G  CE  L   S  D++  +F GQ     +   T+S K ++    E+  RT   Q  +L  G++   ++Y          + L+  + +         +  +YDLGS  V L+S   +   +W  + A R  ++G L++     V GS+      L+  G LW+GG++    P+   + K  GT  GFVGC++++ + ++ + L
Sbjct: 1825 HPCLNGASCLPREASYECLCPGGFSGLHCEKGLIEKSAGDVDALAFDGQTYVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADY----------VALAIVDGR---------LQLTYDLGSQPVVLRSTVPVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLE--KLPIGQALPKAYGT--GFVGCLRDVVVGQRPLHL 2030          

HSP 5 Score: 82.0333 bits (201), Expect = 2.487e-14
Identity = 76/293 (25.94%), Postives = 127/293 (43.34%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT---ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSC---SGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C+PNPC G   C    DG   C C  G  G+ C+  +S  D +    A F G  S++E+K       D  +++ +E++F       GLLLY GQ  +             F+ L+  +         + + F YDLG GA  ++S   +    WT +  +R  + G + +    +V+G +      LNL+  L++GG      P   K+ + A    GF G I+ + +       Q + +  V+R   +     + C   SG+PC N   C+ +   + C C   F+G  C++
Sbjct: 1593 CEPNPCHGAAPCRVLPDGEAKCECPRGRGGAICQ-TVSERDSSQPFLADFHGF-SYLELKGLHTFERDLGEKLALEVVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHD---------RFLEFRYDLGKGAAVIRSKEPVALDTWTRVSLERNGRKGAMRVSDGPRVLGESPVPHTVLNLKEPLYVGGA-----PDFSKLARAAAVFSGFDGAIQLVSLKGH----QLLSQEHVVRSVDVSSFAHHPCTQASGHPCLNGASCLPREASYECLCPGGFSGLHCEK 1855          

HSP 6 Score: 67.3958 bits (163), Expect = 6.575e-10
Identity = 84/303 (27.72%), Postives = 110/303 (36.30%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLC----------------------------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVCSV-TCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            + D  S D  C C    C   +  PVCGSD +TY  EC+L +A C  R  L V +   C    L                             G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G+S C+   C   G   +D     + +T   +CS +      K G   D     PG C T     P  C +  C+FGA C E++    CVC   TC      P   A  VCG DG TY  E  LR  AC+   +I +    PC
Sbjct:  695 VWDEDSEDGPCVCD-FSCHSVLRSPVCGSDGLTYGTECELKKARCESRRELYVTAQGACRGPTLAPLLPAVPPHCTQTPYGCCQDNVTAARGVGLAGCPSSCQC-----NPHGSYSGTCDPTTGQCSCR-PGVGGLKCDRC----EPGFWNFRGIVTD--GRSGCTPCSCDPRGAVRDD----CEQMT--GLCSCKPGVAGPKCGQCPDGHALGPGGCETDSL-APETCAEMHCEFGASCVEEAGSARCVCPTSTC------PGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPC 971          

HSP 7 Score: 58.9214 bits (141), Expect = 2.973e-7
Identity = 31/82 (37.80%), Postives = 45/82 (54.88%), Query Frame = 0
Query:  195 EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSK 276
            E C   +C FGASC+ +A  G + C C    C G     VCGSD +TY NEC L    C + +++++ S  PC+E    G++
Sbjct:  901 ETCAEMHCEFGASCVEEA--GSARCVCPTSTCPGASATKVCGSDGVTYGNECQLRTIACRQGLDISIQSLGPCQETVAPGAR 980          

HSP 8 Score: 50.0618 bits (118), Expect = 1.374e-4
Identity = 55/224 (24.55%), Postives = 82/224 (36.61%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCK-WNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSC 412
            S  + + C  + C FGA C+    +G   C      C      PVCGSD +TY + C L +A C ++  +      PCE+A                  G +      +C  EL                    C Q    W+ED     + G  +C      S  +    PVCG+D +TY + C L+ + C  +R  ++ V   G CR       +P     C
Sbjct:  597 SAGHCQTCGDTVCAFGAVCL----AGQCVCP----RCERPPPGPVCGSDGVTYDSACHLREAACQQQTQIEEARAGPCEQAEC--------------GSGGSGSGEDGECEQEL--------------------CQQHGGVWDED----SEDGPCVCDF----SCHSVLRSPVCGSDGLTYGTECELKKARCESRR--ELYVTAQGACRGPTLAPLLPAVPPHC 768          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592842438|gb|GAXK01115106.1| (TSA: Calanus finmarchicus comp1152792_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 206.838 bits (525), Expect = 5.019e-57
Identity = 128/349 (36.68%), Postives = 187/349 (53.58%), Query Frame = 0
Query:  484 IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG---FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCH 829
            IG+ CL   SDC++ ++ECV   C C  G  P+S     E     S C  +PC+GGGTC+EHDGTF+CYCR G  G  C+  +  TD+  ASF GQ S+V++    +SV R  I++ FR F+    L L        + E+        + +S  N          H+  SYDLG G+VT+ S   I  G W  L  +RY  D  ++L     V G   GS+ +LNL  + +IGGV   D  +M           G  GC+K+LK   + +SL S  EPL++  +G+ EC ++ C+ +PC++ G C +  +G   + C C+R FTG+ C+R+R   C PNPC+++G CH
Sbjct:   12 IGEECL-THSDCVSNHAECVRGECHCRRGFLPSSPLLCKEDP---SPCSSSPCEGGGTCEEHDGTFTCYCREGRLGKFCQEMIGNTDIRVASFQGQ-SYVQLGKIPNSVIRTSIKMRFRIFSSSGVLFL--------SSEHQPGKLGDKLEISVVNT---------HVQLSYDLGGGSVTISSPAPIRLGAWHSLHIQRYRGDASIKLNDHPPVTGRAEGSISSLNLGNYSYIGGVPNRDGSVMQ----------GLEGCVKDLKFGIQPVSLVSRAEPLLVSSQGVGECSKHPCTSSPCRHRGECNTLGRGEKQYKCFCRRGFTGEHCERKRG-HCHPNPCRHDGQCH 959          

HSP 2 Score: 41.9726 bits (97), Expect = 8.354e-3
Identity = 48/204 (23.53%), Postives = 84/204 (41.18%), Query Frame = 0
Query:  798 CHCKRDFTGKSCQRRRNDQ--CDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAY-GSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKD--PGSLG-RLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVD--------GLXAFTAVNIDEXFHILGFKGKD 987
            CHC+R F   S    + D   C  +PC+  G C   D  F C C      G +C     +T     ++ G +        N++   ++ S+ +         V FL  +  PG LG +L+IS   T    +QLS   G  +  I+S P  +     H++ +  YRG+  + ++           + +++N+    +I G   +D
Sbjct:  306 CHCRRGFLPSSPLLCKEDPSPCSSSPCEGGGTCEEHDGTFTCYCREGR-LGKFCQEMIGNTDIRVASFQGQSYVQLGKIPNSV---IRTSIKMRFRIFSSSGVLFLSSEHQPGKLGDKLEISVVNT---HVQLSYDLGGGSVTISS-PAPIRLGAWHSLHIQRYRGDASIKLNDHPPVTGRAEGSISSLNLGNYSYIGGVPNRD 893          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592814217|gb|GAXK01140351.1| (TSA: Calanus finmarchicus comp817742_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 172.17 bits (435), Expect = 3.686e-44
Identity = 137/477 (28.72%), Postives = 210/477 (44.03%), Query Frame = 0
Query:  357 EEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGA-----TR------------IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQ--VQGFLCHCKRDFTGKSCQRRRN 814
            E  PVCG D  TY + C L+   C  ++              I Y +P        D  + C   S+   ++ C+         AG+ C           D +   C+ +++      + C  GA     TR            +G +CL    DC    +EC    C C  G    S   +C       LC  +PC+ GGTC+EHDGTF+C+C  G TG  C+ ++  T    A F GQ S++ +K    SV R  +E+ FRTF   EGL+L          +        F+ +S             H+   YDLGSG   L S   +  G W  LV +RYHQDG+L+++++D V G   G  ++LN++G +++GG      P M +     GT  G  GC+++L+I +K+I L    E + +     V C  + C    C+NEG C ++   +  +C C + F GK C +++ 
Sbjct:  360 EVQPVCGKDGRTYGNACLLKKEECRMQKTI-----------AILYHRPCM------DSVSTCPSHSWLNTTLGCEPCHCSSLGSAGSEC-----------DQQTGQCRCRQQFSGQQCRECEDGAQGCRGTRDTHVNTVTVDASLGQFCL-AHGDCTPRGAECRGGTCHCQLGYKQQSP-IACAPSPP-HLCSFSPCEAGGTCEEHDGTFTCHCGKGRTGKYCQQEVEETAYTEAGFMGQ-SYIALKQGQSSVTRTAVELSFRTFTT-EGLILLATQRESGKGD--------FLSISLVGG---------HVEVRYDLGSGPSVLVSSQAVTLGSWHMLVFRRYHQDGMLQIDKSDPVRGKASGRNKSLNIKGAMYVGG-----HPYMNRTGDLVGTYKGLQGCVRDLRIRRKSIGLVEGVESVGV-----VSCSVHPCKEGYCRNEGQCEAREGRKKAVCSCSKGFRGKRCHKKQG 1610          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592814216|gb|GAXK01140352.1| (TSA: Calanus finmarchicus comp817742_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 169.088 bits (427), Expect = 2.530e-43
Identity = 109/333 (32.73%), Postives = 165/333 (49.55%), Query Frame = 0
Query:  484 IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQ--VQGFLCHCKRDFTGKSCQRRRN 814
            +G +CL    DC    +EC    C C  G    S   +C       LC  +PC+ GGTC+EHDGTF+C+C  G TG  C+ ++  T    A F GQ S++ +K    SV R  +E+ FRTF   EGL+L          +        F+ +S             H+   YDLGSG   L S   +  G W  LV +RYHQDG+L+++++D V G   G  ++LN++G +++GG      P M +     GT  G  GC+++L+I +K+I L    E + +     V C  + C    C+NEG C ++   +  +C C + F GK C +++ 
Sbjct:  360 LGQFCL-AHGDCTPRGAECRGGTCHCQLGYKQQSP-IACAPSPP-HLCSFSPCEAGGTCEEHDGTFTCHCGKGRTGKYCQQEVEETAYTEAGFMGQ-SYIALKQGQSSVTRTAVELSFRTFTT-EGLILLATQRESGKGD--------FLSISLVGG---------HVEVRYDLGSGPSVLVSSQAVTLGSWHMLVFRRYHQDGMLQIDKSDPVRGKASGRNKSLNIKGAMYVGG-----HPYMNRTGDLVGTYKGLQGCVRDLRIRRKSIGLVEGVESVGV-----VSCSVHPCKEGYCRNEGQCEAREGRKKAVCSCSKGFRGKRCHKKQG 1262          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592829622|gb|GAXK01127922.1| (TSA: Calanus finmarchicus comp875548_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 149.443 bits (376), Expect = 5.256e-36
Identity = 87/283 (30.74%), Postives = 142/283 (50.18%), Query Frame = 0
Query:  527 IGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSC 809
            +G+ C  NPCQ GGTC++HDGTF+C+C    +G  C+  +    ++  SF+GQ S++E++       +  I I F+T    +G +LY +   + +  +       F+ L+  N           + F Y+LGSG   L+S  K+  G W  +  KR+HQD +L L+   +V+GS  G L++L+L    +IG       P + +   N GTS G +GC++++K+  +TI      EP+      + EC +  C    C N+G+CI       C C   + G  C
Sbjct: 1423 MGTDCSSNPCQDGGTCEDHDGTFTCFCSEKRSGRFCQTPVYSPKLHIPSFSGQ-SYIELQTMRHVEHKTSIYIEFKTLR-NDGQILYIEQEGLESGRD-------FLSLAIVNG---------FVEFRYNLGSGPAVLRSLKKVIPGMWHSVSVKRWHQDSVLVLDSDPEVLGSVHGDLKSLDLSSSTYIGYAP----PALDQAAMNIGTSKGLIGCMRKIKLGHRTIRFHFDHEPVFKSAFQISECSDKLCQDVTCYNQGVCIEVNGEAKCECAAPYFGDQC 2205          

HSP 2 Score: 88.5817 bits (218), Expect = 5.598e-17
Identity = 82/310 (26.45%), Postives = 129/310 (41.61%), Query Frame = 0
Query:  530 LCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH-----DLSLTDMN--------------------TASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY--GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRR 812
            LC    C   G C E +G   C C +   G +C       D S+ ++N                       FTGQ S +E     +  K  +IE+ F       G+LLY  G+S    +Y          I ++  N    +         SYDLGSG   + S   I    W  +   R   +  L+L+    V G +   + +LNL   L IGG     F  +Y V   +G   G  G I+ + IN +T ++  +R+ L    + + + +   C+GNPC+N+G+C+   +   C C+R + GK C++R
Sbjct:  679 LCQDVTCYNQGVCIEVNGEAKCECAAPYFGDQCSEAIEVPDFSMNEINAIDEKESQKWIPDCSIGECHVPDFTGQ-SFLEFPTLDNVAKYADIEVWFLA-RATNGVLLYNGGKSSGSGDY----------ISINLINGIVQL---------SYDLGSGVAKISSLRPITLNTWHKVRIIRNGLESSLQLDSDPYVSGRSPAPMTSLNLGEPLLIGG-----FRHLYSVSVESGVVRGLDGAIQRVIINGET-TVNLLRKAL--DSKNIRDYKGAPCAGNPCENDGICVPNFRKPACLCRRGYRGKFCKQR 1521          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592749933|gb|GAXK01204480.1| (TSA: Calanus finmarchicus comp206836_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 138.272 bits (347), Expect = 3.789e-33
Identity = 116/382 (30.37%), Postives = 163/382 (42.67%), Query Frame = 0
Query:  508 ICPEGTSPNSAN-TSCEIMKIGSLCDP---NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCK----RDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRG-FVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFD 880
            ICP+G           E+ K   L DP   +PC GGG+C+ HDGTF+CYC  G TG  CE  +    + TA FTGQ S V IK PT    R  + I F+    ++G++++  + +  +  N        I LS +N           + F Y  G G + L S   I  G W  L    YH D +++L   + V+G+     R       + IGG+   D P              F GC+ +++I +  ++L S  EPLV+R  GLV C+   CS   C++         G  CH      R+    S     +  C PNPCQ  G C    R  F C+C G    G  C+   AD      AY          +N + FD
Sbjct:  316 ICPQGQVQQGGRCPESELKKYTDLLDPCSLSPCSGGGSCESHDGTFTCYCMQGRTGKFCEKVVMTDHIETAGFTGQ-SFVLIKNPTSDGPRSSLMIKFKPHR-KDGVIMFSSTDSDHSVGN--------ISLSLTNG---------FVQFIYQEGEGNLNLLSSSSITLGHWHSLALHTYHGDAMMQLGEEEPVVGTFENG-RIRGFGPSIKIGGI-TTDSP------------DNFHGCLSDVRIGRHPVNLVSHAEPLVVRTVGLVSCDHIECSETACES---------GDNCHSADGKCREGARSSHTIATSGPCHPNPCQGGGRCLRGQRDMFQCLC-GVGRVGRLCETSLADVMMADQAY----LKDAVIDNLMVFD 1320          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592749935|gb|GAXK01204478.1| (TSA: Calanus finmarchicus comp206836_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 137.887 bits (346), Expect = 9.267e-33
Identity = 114/382 (29.84%), Postives = 163/382 (42.67%), Query Frame = 0
Query:  508 ICPEGTSPNSAN-TSCEIMKIGSLCDP---NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCK----RDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRG-FVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFD 880
            ICP+G           E+ K   L DP   +PC GGG+C+ HDGTF+CYC  G TG  CE  +    + TA FTGQ S V IK PT    R  + I F+    ++G++++  + +  +  N        I LS +N           + F Y  G G + L S   I  G W  L    YH D +++L   + V+G+     R       + IGG+  +             +   F GC+ +++I +  ++L S  EPLV+R  GLV C+   CS   C++         G  CH      R+    S     +  C PNPCQ  G C    R  F C+C G    G  C+   AD      AY          +N + FD
Sbjct:  316 ICPQGQVQQGGRCPESELKKYTDLLDPCSLSPCSGGGSCESHDGTFTCYCMQGRTGKFCEKVVMTDHIETAGFTGQ-SFVLIKNPTSDGPRSSLMIKFKPHR-KDGVIMFSSTDSDHSVGN--------ISLSLTNG---------FVQFIYQEGEGNLNLLSSSSITLGHWHSLALHTYHGDAMMQLGEEEPVVGTFENG-RIRGFGPSIKIGGITTD-------------SPDNFHGCLSDVRIGRHPVNLVSHAEPLVVRTVGLVSCDHIECSETACES---------GDNCHSADGKCREGARSSHTIATSGPCHPNPCQGGGRCLRGQRDMFQCLC-GVGRVGRLCETSLADVMMADQAY----LKDAVIDNLMVFD 1320          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592749934|gb|GAXK01204479.1| (TSA: Calanus finmarchicus comp206836_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 124.79 bits (312), Expect = 1.205e-28
Identity = 105/348 (30.17%), Postives = 149/348 (42.82%), Query Frame = 0
Query:  538 GGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCK----RDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRG-FVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFD 880
            GGG+C+ HDGTF+CYC  G TG  CE  +    + TA FTGQ S V IK PT    R  + I F+    ++G++++  + +  +  N        I LS +N           + F Y  G G + L S   I  G W  L    YH D +++L   + V+G+     R       + IGG+   D P              F GC+ +++I +  ++L S  EPLV+R  GLV C+   CS   C++         G  CH      R+    S     +  C PNPCQ  G C    R  F C+C G    G  C+   AD      AY          +N + FD
Sbjct:  487 GGGSCESHDGTFTCYCMQGRTGKFCEKVVMTDHIETAGFTGQ-SFVLIKNPTSDGPRSSLMIKFKPHR-KDGVIMFSSTDSDHSVGN--------ISLSLTNG---------FVQFIYQEGEGNLNLLSSSSITLGHWHSLALHTYHGDAMMQLGEEEPVVGTFENG-RIRGFGPSIKIGGI-TTDSP------------DNFHGCLSDVRIGRHPVNLVSHAEPLVVRTVGLVSCDHIECSETACES---------GDNCHSADGKCREGARSSHTIATSGPCHPNPCQGGGRCLRGQRDMFQCLC-GVGRVGRLCETSLADVMMADQAY----LKDAVIDNLMVFD 1389          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592749936|gb|GAXK01204477.1| (TSA: Calanus finmarchicus comp206836_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 124.79 bits (312), Expect = 2.130e-28
Identity = 105/348 (30.17%), Postives = 149/348 (42.82%), Query Frame = 0
Query:  538 GGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCK----RDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRG-FVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFD 880
            GGG+C+ HDGTF+CYC  G TG  CE  +    + TA FTGQ S V IK PT    R  + I F+    ++G++++  + +  +  N        I LS +N           + F Y  G G + L S   I  G W  L    YH D +++L   + V+G+     R       + IGG+   D P              F GC+ +++I +  ++L S  EPLV+R  GLV C+   CS   C++         G  CH      R+    S     +  C PNPCQ  G C    R  F C+C G    G  C+   AD      AY          +N + FD
Sbjct:  487 GGGSCESHDGTFTCYCMQGRTGKFCEKVVMTDHIETAGFTGQ-SFVLIKNPTSDGPRSSLMIKFKPHR-KDGVIMFSSTDSDHSVGN--------ISLSLTNG---------FVQFIYQEGEGNLNLLSSSSITLGHWHSLALHTYHGDAMMQLGEEEPVVGTFENG-RIRGFGPSIKIGGI-TTDSP------------DNFHGCLSDVRIGRHPVNLVSHAEPLVVRTVGLVSCDHIECSETACES---------GDNCHSADGKCREGARSSHTIATSGPCHPNPCQGGGRCLRGQRDMFQCLC-GVGRVGRLCETSLADVMMADQAY----LKDAVIDNLMVFD 1389          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592750032|gb|GAXK01204381.1| (TSA: Calanus finmarchicus comp207306_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 118.627 bits (296), Expect = 6.116e-26
Identity = 124/488 (25.41%), Postives = 214/488 (43.85%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHD-GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGS--GAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNA--GTSIGFVGCIKELKINKKTISLQSMREPLVIRRRG--LVECEENSCSGNPCKNEGLCI---SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD--RGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISST---------KTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVN 973
            C CP+G S  S +    +   G+    + CQ G TC  +  G +SC C  G  G  CE ++ L+D   A FTG  S++ + + T       + +  +  N  +GL+L+     +S   N    F   + ++ +            + F+Y LGS     T+++   IE   W  + A RY   G L+L+ T     ST G L TL++   +++GG+     P M  + + +  G S  F GC++ L IN+    L+ M       R+G  +V+C+   C G  C+N+G+C+       G+ C C+  +TG  CQ      C  +PC+N   C + +    F C C    Y G  CD +              + P     + L ++L     +     +E+++ +  K+ G L  + I  T         + GLLK+  +    + TE +T P   ++D+  H + +   R ++D+ +D L   + V+
Sbjct: 4416 CQCPDGRSGPSCDMHHNVCSGGA----HQCQEGSTCITNSTGGYSCVCPLGRRGQHCEENVGLSD---ARFTGDHSYMSVDLMTAVRFNTHVILEIKPEN-SDGLILH-----LSQRTNQFGDFLSLLLVNGT------------LFFTYSLGSDESVTTIRAPCCIELNTWHTISAGRYGNQGYLQLDETFMKGQSTEG-LLTLDVSPTIYLGGL-----PDMAAISEKSIEGKSSRFKGCLRNLHINENAYPLRVMS-----GRQGANIVDCDGTVCGGEVCQNDGVCVLDKDTSVGYHCECQNRYTGNHCQLHT--LCQNSPCKNKAKCKVFNGKHDFFCDCPLG-YQGKTCDSRI-----------DVEVPHFGGQSYLQYELPDVNTVRYSTEIEMDI-YSDKNNGMLVWIGIDPTADDYLGVGLEDGLLKVVWNLGWFSRTE-LTIPDRNLTDDSWHKVHIKRMRQQMDILLDELSYISQVH 5723          

HSP 2 Score: 87.8113 bits (216), Expect = 1.352e-16
Identity = 91/384 (23.70%), Postives = 162/384 (42.19%), Query Frame = 0
Query:  529 SLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKR--MEIEIMFRTFNVQEGLLLY----GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG--FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK--DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKK 902
            + C  N C+ GGTC      F C+CR   +G  CE ++ +    T  F G    V +    +      ++IEI F+T +  EG+L Y    G+    S  ++ I  F       S  ++   F + KH + + +  + A+ +  +   + G     V K         L  T  + G    +     L  ++++GG  P+D  L++        S G  GC++   +++K + L +      +  + ++EC+   C+ +PC N G+C   + G  + C C   FTG  C+R     C+ NPC + G C  K    GF+C+C     +G    +   +   +QP++ S     + F   L +     +GL  EA      + + +
Sbjct: 6231 TACKNNVCRNGGTCLTSIDGFQCHCRLQYSGRHCEQEVIV---KTPGFVGHSYLVHLLNTNNKTVEDGVDIEIKFKTRH-SEGILFYSGQEGKMFITSYLQDGILHFKV-----SCGHQLITFSDPKHRVDNGNTHTLALMVALNKDTDNGHTCRTVIK---------LNGTHTMRGEQE-TYWVPQLPEYIYLGGA-PHDM-LIFHDADMIIPSYGLRGCVRSFMLDEKELDLFAD----AVTGQDIIECDSAVCAESPCHNNGVCNQDLTGTSWFCDCLPGFTGPLCERPV---CNANPCLHGGTCIGKGDGDGFICLCP----FGRRGGICEEEIKLNQPSFTSWVVGYSSF---LTYMTPPDVGLYLEAKFHFTTNVVNQ 7277          

HSP 3 Score: 87.4261 bits (215), Expect = 2.064e-16
Identity = 82/320 (25.62%), Postives = 131/320 (40.94%), Query Frame = 0
Query:  530 LCDPNPCQGGGTC---DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFT----GQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLV-AKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRND-QCDPNPCQNNGVCHIKDR-GFVCVC 839
            +C+ NPC  GGTC    + DG F C C  G  G  CE ++ L   N  SFT    G  S +    P D    +E +  F T  V +  LL       ++ + +      FI                H++ ++DLG+G   + +   +++    H V   R  + G ++++    + G   G L  LN+ G  +IGG +  +F    ++  +     GF GCI +L +    ++      P+  R R + +C +  C   PC+  G C+     F C C    +G SC    N      + CQ    C      G+ CVC
Sbjct: 5592 VCNANPCLHGGTCIGKGDGDG-FICLCPFGRRGGICEEEIKL---NQPSFTSWVVGYSSFLTYMTPPDVGLYLEAKFHFTTNVVNQVALLMFMGQKGTHRQGSDYLAVSFIK--------------GHVLLTWDLGAGPRRIFTPAPVDERLLVHSVHIGRRGRRGWMKVDHFKNITGLAPGKLADLNVNGEFFIGGHELYNFT---RLPHDLPVHTGFTGCIFDLVLKGGDMNPIV---PIPQRGRNVQQCHQRMCDREPCQG-GYCLDYGASFSCQCPDGRSGPSCDMHHNVCSGGAHQCQEGSTCITNSTGGYSCVC 6476          

HSP 4 Score: 60.077 bits (144), Expect = 4.236e-8
Identity = 33/82 (40.24%), Postives = 44/82 (53.66%), Query Frame = 0
Query:  773 CEEN--SCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLK 852
            CE N   C  NPC N G C+  +  F C C   +TG+ C+ R  +QC+ NPC N  +C I+D  F C C   +Y+G  C  K
Sbjct: 8496 CEVNVDECEENPCLNGGHCMDLIGDFSCTCSITWTGRLCEERV-EQCEDNPCLNGALCLIEDDSFRCYCV-PDYHGKRCQYK 8735          

HSP 5 Score: 57.3806 bits (137), Expect = 2.902e-7
Identity = 35/89 (39.33%), Postives = 44/89 (49.44%), Query Frame = 0
Query:  773 CEE--NSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRND-QCDPNP-CQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTP 857
            CEE    C  NPC N  LC+ +   F C+C  D+ GK CQ + N+ Q  P P C + G C      F C C  S + G YC+ K  D P
Sbjct: 8358 CEERVEQCEDNPCLNGALCLIEDDSFRCYCVPDYHGKRCQYKYNECQLPPGPKCLHGGTCQDDVDDFECSCE-SGFSGIYCECKDGDEP 8621          

HSP 6 Score: 47.3654 bits (111), Expect = 3.036e-4
Identity = 25/68 (36.76%), Postives = 32/68 (47.06%), Query Frame = 0
Query:  772 ECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            E + + C  +PC N G C   +  F C C   F+G  CQ   +D C   PC NNG C     G+ C C
Sbjct: 8895 ETDWDECWNSPCLNGGTCHDGIAHFNCTCPTGFSGSRCQVNVDD-CLSEPCWNNGTCEDGVDGYSCNC 9095          

HSP 7 Score: 45.4394 bits (106), Expect = 1.201e-3
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = 0
Query:  775 ENSCSGNPCKNEGLCISQV---QGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTAD 855
            +N C   PC++ G+C+  V   +G+ C C+  +TG +C+    D+C  +PC N G CH     F C C  + + G+ C +   D
Sbjct: 8964 DNPCIHKPCEH-GVCVETVGVGRGYKCFCEDGYTGYNCETDW-DECWNSPCLNGGTCHDGIAHFNCTCP-TGFSGSRCQVNVDD 9206          

HSP 8 Score: 36.1946 bits (82), Expect = 7.350e-1
Identity = 19/58 (32.76%), Postives = 30/58 (51.72%), Query Frame = 0
Query:  506 RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            +C C +G +  +  T  +       C  +PC  GGTC +    F+C C +G +GSRC+
Sbjct: 8979 KCFCEDGYTGYNCETDWD------ECWNSPCLNGGTCHDGIAHFNCTCPTGFSGSRCQ 9134          

HSP 9 Score: 35.8094 bits (81), Expect = 9.873e-1
Identity = 26/82 (31.71%), Postives = 38/82 (46.34%), Query Frame = 0
Query:  501 ECVNNRCI----CPEGTSPNSANTSCEIMKIGSLCDPN-------PCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDM 571
            EC N+ C+    C +G +    N +C     GS C  N       PC   GTC++    +SC C  G  G+ CE D+ + +M
Sbjct: 8841 ECWNSPCLNGGTCHDGIA--HFNCTCPTGFSGSRCQVNVDDCLSEPCWNNGTCEDGVDGYSCNCLLGFRGANCEIDIQVCNM 9080          

HSP 10 Score: 35.8094 bits (81), Expect = 1.047e+0
Identity = 23/57 (40.35%), Postives = 27/57 (47.37%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            CIC  G     A  +CE+      C+ NPC  GG C +  G FSC C    TG  CE
Sbjct: 8616 CICENGW----AGETCEVNV--DECEENPCLNGGHCMDLIGDFSCTCSITWTGRLCE 8768          

HSP 11 Score: 34.6538 bits (78), Expect = 2.581e+0
Identity = 18/51 (35.29%), Postives = 23/51 (45.10%), Query Frame = 0
Query:  521 SCEIMKIGSLCDP-------NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            +C I   G LC+        NPC  G  C   D +F CYC     G RC++
Sbjct: 8499 TCSITWTGRLCEERVEQCEDNPCLNGALCLIEDDSFRCYCVPDYHGKRCQY 8651          
BLAST of EMLSAG00000004167 vs. C. finmarchicus
Match: gi|592750031|gb|GAXK01204382.1| (TSA: Calanus finmarchicus comp207306_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 118.627 bits (296), Expect = 6.166e-26
Identity = 124/488 (25.41%), Postives = 214/488 (43.85%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHD-GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGS--GAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNA--GTSIGFVGCIKELKINKKTISLQSMREPLVIRRRG--LVECEENSCSGNPCKNEGLCI---SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD--RGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISST---------KTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVN 973
            C CP+G S  S +    +   G+    + CQ G TC  +  G +SC C  G  G  CE ++ L+D   A FTG  S++ + + T       + +  +  N  +GL+L+     +S   N    F   + ++ +            + F+Y LGS     T+++   IE   W  + A RY   G L+L+ T     ST G L TL++   +++GG+     P M  + + +  G S  F GC++ L IN+    L+ M       R+G  +V+C+   C G  C+N+G+C+       G+ C C+  +TG  CQ      C  +PC+N   C + +    F C C    Y G  CD +              + P     + L ++L     +     +E+++ +  K+ G L  + I  T         + GLLK+  +    + TE +T P   ++D+  H + +   R ++D+ +D L   + V+
Sbjct: 3954 CQCPDGRSGPSCDMHHNVCSGGA----HQCQEGSTCITNSTGGYSCVCPLGRRGQHCEENVGLSD---ARFTGDHSYMSVDLMTAVRFNTHVILEIKPEN-SDGLILH-----LSQRTNQFGDFLSLLLVNGT------------LFFTYSLGSDESVTTIRAPCCIELNTWHTISAGRYGNQGYLQLDETFMKGQSTEG-LLTLDVSPTIYLGGL-----PDMAAISEKSIEGKSSRFKGCLRNLHINENAYPLRVMS-----GRQGANIVDCDGTVCGGEVCQNDGVCVLDKDTSVGYHCECQNRYTGNHCQLHT--LCQNSPCKNKAKCKVFNGKHDFFCDCPLG-YQGKTCDSRI-----------DVEVPHFGGQSYLQYELPDVNTVRYSTEIEMDI-YSDKNNGMLVWIGIDPTADDYLGVGLEDGLLKVVWNLGWFSRTE-LTIPDRNLTDDSWHKVHIKRMRQQMDILLDELSYISQVH 5261          

HSP 2 Score: 87.8113 bits (216), Expect = 1.344e-16
Identity = 91/384 (23.70%), Postives = 162/384 (42.19%), Query Frame = 0
Query:  529 SLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKR--MEIEIMFRTFNVQEGLLLY----GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG--FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK--DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKK 902
            + C  N C+ GGTC      F C+CR   +G  CE ++ +    T  F G    V +    +      ++IEI F+T +  EG+L Y    G+    S  ++ I  F       S  ++   F + KH + + +  + A+ +  +   + G     V K         L  T  + G    +     L  ++++GG  P+D  L++        S G  GC++   +++K + L +      +  + ++EC+   C+ +PC N G+C   + G  + C C   FTG  C+R     C+ NPC + G C  K    GF+C+C     +G    +   +   +QP++ S     + F   L +     +GL  EA      + + +
Sbjct: 5769 TACKNNVCRNGGTCLTSIDGFQCHCRLQYSGRHCEQEVIV---KTPGFVGHSYLVHLLNTNNKTVEDGVDIEIKFKTRH-SEGILFYSGQEGKMFITSYLQDGILHFKV-----SCGHQLITFSDPKHRVDNGNTHTLALMVALNKDTDNGHTCRTVIK---------LNGTHTMRGEQE-TYWVPQLPEYIYLGGA-PHDM-LIFHDADMIIPSYGLRGCVRSFMLDEKELDLFAD----AVTGQDIIECDSAVCAESPCHNNGVCNQDLTGTSWFCDCLPGFTGPLCERPV---CNANPCLHGGTCIGKGDGDGFICLCP----FGRRGGICEEEIKLNQPSFTSWVVGYSSF---LTYMTPPDVGLYLEAKFHFTTNVVNQ 6815          

HSP 3 Score: 87.4261 bits (215), Expect = 2.177e-16
Identity = 82/320 (25.62%), Postives = 131/320 (40.94%), Query Frame = 0
Query:  530 LCDPNPCQGGGTC---DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFT----GQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLV-AKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRND-QCDPNPCQNNGVCHIKDR-GFVCVC 839
            +C+ NPC  GGTC    + DG F C C  G  G  CE ++ L   N  SFT    G  S +    P D    +E +  F T  V +  LL       ++ + +      FI                H++ ++DLG+G   + +   +++    H V   R  + G ++++    + G   G L  LN+ G  +IGG +  +F    ++  +     GF GCI +L +    ++      P+  R R + +C +  C   PC+  G C+     F C C    +G SC    N      + CQ    C      G+ CVC
Sbjct: 5130 VCNANPCLHGGTCIGKGDGDG-FICLCPFGRRGGICEEEIKL---NQPSFTSWVVGYSSFLTYMTPPDVGLYLEAKFHFTTNVVNQVALLMFMGQKGTHRQGSDYLAVSFIK--------------GHVLLTWDLGAGPRRIFTPAPVDERLLVHSVHIGRRGRRGWMKVDHFKNITGLAPGKLADLNVNGEFFIGGHELYNFT---RLPHDLPVHTGFTGCIFDLVLKGGDMNPIV---PIPQRGRNVQQCHQRMCDREPCQG-GYCLDYGASFSCQCPDGRSGPSCDMHHNVCSGGAHQCQEGSTCITNSTGGYSCVC 6014          

HSP 4 Score: 60.077 bits (144), Expect = 4.292e-8
Identity = 33/82 (40.24%), Postives = 44/82 (53.66%), Query Frame = 0
Query:  773 CEEN--SCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLK 852
            CE N   C  NPC N G C+  +  F C C   +TG+ C+ R  +QC+ NPC N  +C I+D  F C C   +Y+G  C  K
Sbjct: 8034 CEVNVDECEENPCLNGGHCMDLIGDFSCTCSITWTGRLCEERV-EQCEDNPCLNGALCLIEDDSFRCYCV-PDYHGKRCQYK 8273          

HSP 5 Score: 55.4546 bits (132), Expect = 9.670e-7
Identity = 34/89 (38.20%), Postives = 44/89 (49.44%), Query Frame = 0
Query:  773 CEE--NSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRND-QCDPNP-CQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTP 857
            CEE    C  NPC N  LC+ +   F C+C  D+ GK CQ + N+ Q  P P C + G C      F C C  S + G YC+ K  + P
Sbjct: 7896 CEERVEQCEDNPCLNGALCLIEDDSFRCYCVPDYHGKRCQYKYNECQLPPGPKCLHGGTCQDDVDDFECSCE-SGFSGIYCECKDGEEP 8159          

HSP 6 Score: 47.3654 bits (111), Expect = 3.105e-4
Identity = 25/68 (36.76%), Postives = 32/68 (47.06%), Query Frame = 0
Query:  772 ECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            E + + C  +PC N G C   +  F C C   F+G  CQ   +D C   PC NNG C     G+ C C
Sbjct: 8433 ETDWDECWNSPCLNGGTCHDGIAHFNCTCPTGFSGSRCQVNVDD-CLSEPCWNNGTCEDGVDGYSCNC 8633          

HSP 7 Score: 45.4394 bits (106), Expect = 1.239e-3
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = 0
Query:  775 ENSCSGNPCKNEGLCISQV---QGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTAD 855
            +N C   PC++ G+C+  V   +G+ C C+  +TG +C+    D+C  +PC N G CH     F C C  + + G+ C +   D
Sbjct: 8502 DNPCIHKPCEH-GVCVETVGVGRGYKCFCEDGYTGYNCETDW-DECWNSPCLNGGTCHDGIAHFNCTCP-TGFSGSRCQVNVDD 8744          

HSP 8 Score: 36.1946 bits (82), Expect = 7.274e-1
Identity = 19/58 (32.76%), Postives = 30/58 (51.72%), Query Frame = 0
Query:  506 RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            +C C +G +  +  T  +       C  +PC  GGTC +    F+C C +G +GSRC+
Sbjct: 8517 KCFCEDGYTGYNCETDWD------ECWNSPCLNGGTCHDGIAHFNCTCPTGFSGSRCQ 8672          

HSP 9 Score: 35.8094 bits (81), Expect = 9.854e-1
Identity = 26/82 (31.71%), Postives = 38/82 (46.34%), Query Frame = 0
Query:  501 ECVNNRCI----CPEGTSPNSANTSCEIMKIGSLCDPN-------PCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDM 571
            EC N+ C+    C +G +    N +C     GS C  N       PC   GTC++    +SC C  G  G+ CE D+ + +M
Sbjct: 8379 ECWNSPCLNGGTCHDGIA--HFNCTCPTGFSGSRCQVNVDDCLSEPCWNNGTCEDGVDGYSCNCLLGFRGANCEIDIQVCNM 8618          

HSP 10 Score: 35.8094 bits (81), Expect = 1.037e+0
Identity = 23/57 (40.35%), Postives = 27/57 (47.37%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            CIC  G     A  +CE+      C+ NPC  GG C +  G FSC C    TG  CE
Sbjct: 8154 CICENGW----AGETCEVNV--DECEENPCLNGGHCMDLIGDFSCTCSITWTGRLCE 8306          

HSP 11 Score: 34.6538 bits (78), Expect = 2.576e+0
Identity = 18/51 (35.29%), Postives = 23/51 (45.10%), Query Frame = 0
Query:  521 SCEIMKIGSLCDP-------NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            +C I   G LC+        NPC  G  C   D +F CYC     G RC++
Sbjct: 8037 TCSITWTGRLCEERVEQCEDNPCLNGALCLIEDDSFRCYCVPDYHGKRCQY 8189          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000004167 (pep:novel supercontig:LSalAtl2s:LSalAtl2s220:203389:278765:1 gene:EMLSAG00000004167 transcript:EMLSAT00000004167 description:"maker-LSalAtl2s220-augustus-gene-2.5")

HSP 1 Score: 2028.06 bits (5253), Expect = 0.000e+0
Identity = 995/995 (100.00%), Postives = 995/995 (100.00%), Query Frame = 0
Query:    1 MKSSKSTKVITPRNVDLPPHVLSKDFASGPQGPFPRKKCLCLSLWLLGLLLLCIGAVAALNFVLPDVLLSSNGGVPEVYGLMRSEECPNEDGSSLDYRVSASDVILVGSKSPLLSGEEDEGGLRAERVLKGSLMRRELFGGPCAVSLSEGKRVFFLEHVDESLRLKFPSLLASDKLIRVIRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVNIDEXFHILGFKGKDLQGDRMDV 995
            MKSSKSTKVITPRNVDLPPHVLSKDFASGPQGPFPRKKCLCLSLWLLGLLLLCIGAVAALNFVLPDVLLSSNGGVPEVYGLMRSEECPNEDGSSLDYRVSASDVILVGSKSPLLSGEEDEGGLRAERVLKGSLMRRELFGGPCAVSLSEGKRVFFLEHVDESLRLKFPSLLASDKLIRVIRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVNIDEXFHILGFKGKDLQGDRMDV
Sbjct:    1 MKSSKSTKVITPRNVDLPPHVLSKDFASGPQGPFPRKKCLCLSLWLLGLLLLCIGAVAALNFVLPDVLLSSNGGVPEVYGLMRSEECPNEDGSSLDYRVSASDVILVGSKSPLLSGEEDEGGLRAERVLKGSLMRRELFGGPCAVSLSEGKRVFFLEHVDESLRLKFPSLLASDKLIRVIRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVNIDEXFHILGFKGKDLQGDRMDV 995          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000003993 (pep:novel supercontig:LSalAtl2s:LSalAtl2s213:441137:447252:-1 gene:EMLSAG00000003993 transcript:EMLSAT00000003993 description:"maker-LSalAtl2s213-snap-gene-4.21")

HSP 1 Score: 104.375 bits (259), Expect = 9.366e-23
Identity = 117/475 (24.63%), Postives = 199/475 (41.89%), Query Frame = 0
Query:  530 LCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLS--LTDMNTAS------------------------FTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGS-------GAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-----ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDG 965
            +C  +PC  GGTC     +F C C  G  G  C  D S   T M+  +                        FTG  S+++  + +       I I  R   ++  ++  GQS N  + +        F+ L   N           ++FS+ LG          VT+++   +E  KW  + A RY + G L  +      GST   L TL++   L++GG+  +    +  V K+      + GCI+ L IN+K+  L S       +   + +C+  +C G  C+N G C     I   +G+ C C  ++ G+ C++ R   C  N C+N+G+C I D   + C C    Y+G++C        +D+      + P+ + +  ++F L              E   ++ D  S   L +   K G++ L  +    + TE   + P  V+D++ H ++++  R EL LDVDG
Sbjct:  989 ICSSSPCSNGGTCIGXGASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPFGKNPLFTGHGSYMQTHLKSAIRFNTHIHIEIRPQTLEGHIIHLGQSRNGRHQD--------FLSLLLVNGS---------VVFSFSLGGPIGGNVGNTVTIRT-CCVEIDKWYSVEAGRYGRKGYLRXD-GQFTTGSTESGLNTLDVDQELFLGGIPNSHMLFIDFVSKHY-----YEGCIRNLHINEKSYPLTSTGNHW--KGWNIKDCDGTACGGELCQNGGKCSLSENIHNHRGYECGCPDNYIGEHCEQHR--LC-INACENDGICSIGDDEIIHCDCPLG-YHGSFC--------SDEE---EIRVPRFYGDGFISFVLS-------------ETKSIRHDQTSDDYLGV-GLKDGMVHLVWNLGWFSRTEL--TVPSKVNDDKWHRVLIDRIRQELKLDVDG 1406          

HSP 2 Score: 80.4925 bits (197), Expect = 1.652e-15
Identity = 88/347 (25.36%), Postives = 142/347 (40.92%), Query Frame = 0
Query:  505 NRCICPEGTSPNSANTSCEIMKIGSLCD-----PNPCQGGGTC--DEHDGTFSCYCRSGLTGSRCE------------------HDLSLTDMNTASFT----GQDSHVEIKIPTDSV-KRMEIEIMFRTFNV-QEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVA-KRYHQDGLLELERTDKVIGSTRGSLRTLNLQG-HLWIGGVDPNDFPLM---YKVRKNAGTSIGFVGCIKELKINKKT------ISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSC 809
            N  IC +     S    C     G+LC+      NPCQ GGTC   +    F C C++G  GS CE                  + L   ++   SF     G  S++   + T +V K +E +  F T N  Q  LL +     + ++ +       FI LS             HI+ ++DLG+G   + +   +++  + H V   R  + G L+++    + G + G    LN+   + +IGG +  +F L+   + V +      GF GC+ ++            ++L S      IR R + +C  + CS +PC N G CI     FLC C     G +C
Sbjct:  696 NGAICAQHEDSGSWFCDCPPGFTGALCERPVCQTNPCQYGGTCLGSKTGSGFLCLCQAGRRGSLCEEGNQIQVYIKRLNYKAYEYFLPEVELTQPSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSD------FIALSYIKG---------HILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHR------GFSGCMFDMAYRSYVTGQLTPLALPSKIGSSPIRGRNVEQCHYDICSSSPCSNGGTCIGXGASFLCECTDGRYGITC 1021          

HSP 3 Score: 72.7886 bits (177), Expect = 4.026e-13
Identity = 79/326 (24.23%), Postives = 127/326 (38.96%), Query Frame = 0
Query:  530 LCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVK--RMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLK---SHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMY-------KVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQ--GFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVC--HIKDRGFVCVC 839
            +C  N C  GGTC        C C     G +CE ++ +    T  F G    V      + VK     +++ FRT + +EGL+L+                                +++ +++ S  +  G +T K    H  I      H V   Y Q   + +   D +  ++     T  + G   I  + P    ++Y       K   N   ++GF GC+K L++N   ++L   R+   +  R +VEC+   C+  PC+N  +C        + C C   FTG  C+R     C  NPCQ  G C       GF+C+C
Sbjct:  468 ICTSNVCSNGGTCLTTLSGVKCICPLQYHGVQCEEEVFI---ETPGFVGHSGLVHKLSKNNIVKDRNFHVQLSFRT-SQEEGLILF------------------------------TGEDVGYLLVSGYIKDGILTFKISCGHQIILFSDPRHRVDXGYLQXLSINITIFDTICSTSIQLNETRTMSGDQQIDSL-PQIPDMLYMGLVPHKKELGNGIINVGFQGCMKHLQLNSNELNL--YRD--AVDGRDIVECKSAICAIQPCRNGAICAQHEDSGSWFCDCPPGFTGALCER---PVCQTNPCQYGGTCLGSKTGSGFLCLC 751          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000012366 (pep:novel supercontig:LSalAtl2s:LSalAtl2s903:98980:175896:1 gene:EMLSAG00000012366 transcript:EMLSAT00000012366 description:"maker-LSalAtl2s903-augustus-gene-1.44")

HSP 1 Score: 85.1149 bits (209), Expect = 5.331e-17
Identity = 92/331 (27.79%), Postives = 131/331 (39.58%), Query Frame = 0
Query:  194 VEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG-------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKE----IVVADFKPCTRGATRIGDYCLHEESDCLALNSE 501
            V  C+  NC +G  C + +K G + C C    C   I +P+CGSD ITY+N C L ++ C KR  ++ I    C     C  + C     CV +                    G N K+Y + C +  +AC     +LT+    +C  C+     N  C +     K  C CP       +    VCGTD+ITY S C L+ + C              +CR I           +C +GA C  D  CVC   C   E+        +CG D  TY  E ++ +  C  + E    I      PC R +T++       E  C  L SE
Sbjct:  477 VNPCKLLNCDWGQECEI-SKQGITNCVCPQ-QCEPII-RPICGSDRITYENLCSLRKSACEKRKIISPIHYGICGSNGPCSKRKCNHNSICVESEDLAFCECPSCESLEYHPVCGTNGKMYSNSCQLSREACLKEDDSLTVGDIKSCSGCA-----NVTCDFHGYCHKGECHCPSLCDNEIDHVGSVCGTDDITYPSECQLKITACH-------------ICRGI-----------NCQYGAKC-LDGVCVCPTNCPRHEE-------RLCGTDYMTYLNECEMLKATCTAKNEGKIPISFLHHGPC-RSSTKM------TECHCSELGSE 760          

HSP 2 Score: 60.8474 bits (146), Expect = 1.656e-9
Identity = 86/342 (25.15%), Postives = 137/342 (40.06%), Query Frame = 0
Query:  186 TEASSGSNVE----------GCRHSN-CRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRT-GGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLY-------------SEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEED----SYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI------VVADFKPCTRGATRIGDYCLHEE 492
            TE+SS SN +           C   N C FG  C+      DS  +C+  D       PVCGSD +TY NEC +  A C K+  L  +    C   + C    CP    C+ +  G++ K    +   + +   + +  L +     C  CS    +N D L               E+  + +C+     +  +++  PVC ++  TY + C ++  +C  +R  D+ + YP  C     +   PC   +CD+G  CE      + CVC   C+            +CG D  TYE    LR+ AC+ +K I      +     PC++        C+  E
Sbjct:  252 TESSSSSNAKQQHTRLQIAKSCDDENPCPFGGVCV---HMEDSTIECQCKDTCPYAYTPVCGSDQVTYDNECKMKLAICRKKKRLKKVHSGIC-GVNSCLKLSCPDYSRCIPSYDGSSAKCVCPEPCKDSNNRSAIVCGLEVRYKGPCNPCS---GFNCDVLRFQECHVLPIDQDKQERIPRCMCN-----TDCSDKTSPVCASNGQTYKNDCYMKLDSC--RRLLDLHIVYPDTCS----KGVNPCKLLNCDWGQECEISKQGITNCVCPQQCEP-------IIRPICGSDRITYENLCSLRKSACEKRKIISPIHYGICGSNGPCSKRKCNHNSICVESE 568          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000007498 (pep:novel supercontig:LSalAtl2s:LSalAtl2s430:288057:377591:1 gene:EMLSAG00000007498 transcript:EMLSAT00000007498 description:"maker-LSalAtl2s430-augustus-gene-4.9")

HSP 1 Score: 71.633 bits (174), Expect = 6.104e-13
Identity = 84/317 (26.50%), Postives = 125/317 (39.43%), Query Frame = 0
Query:  535 PCQGGGTC----DEHD-------GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTD-----KVIGSTR---GSLRTLNLQGHLWIGGVDPNDFPLMY-KVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEE----------NSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNP 821
            PCQ  G C      H+       GT S   R G+              N  SF G    V   I   S    EIE++F++ + ++GL+ Y       N +    T  F   L S N          ++ F+YDLG G   ++S   I    W  +V  R  +   +E+ R       K+  S+R   G+   L L+ +L++GGV   D    Y  +R++      F GCI++L IN   I+L +      I    +  C+           N    +PC   G C   + GF C C    TG SCQ+   +  +P P
Sbjct:  313 PCQHNGKCVPLESGHNIWWAINCGTLSISLRKGIISK-----------NVPSFNGTSYLVYTGIGDSSPLWTEIELVFQS-SAEDGLIFY-------NGQRNDGTDDFIAILLSRN----------YVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAYLPIRRS------FFGCIQKLTINDVEINLVNS----AISGVDVGSCDHPCNTPSSSSSNEFPQHPCGVHGNCKPVLDGFKCDCPHGLTGNSCQKITGESNNPLP 590          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000003184 (pep:novel supercontig:LSalAtl2s:LSalAtl2s176:575372:647076:1 gene:EMLSAG00000003184 transcript:EMLSAT00000003184 description:"augustus_masked-LSalAtl2s176-processed-gene-6.1")

HSP 1 Score: 68.1662 bits (165), Expect = 7.527e-12
Identity = 105/399 (26.32%), Postives = 158/399 (39.60%), Query Frame = 0
Query:  463 CKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNS---------ECVNNRC----ICPEGTSPNS-------ANTSCEIMKIGSLCDP--NPCQ----GGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY---GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAV-TLKSHGKIEKGKWTHLVAKRYHQDGLLELE-----RTDKVIGST---RGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECE-------ENSCSGNPCKNEGLCISQVQG--FLCHCKRDFTGKSCQRR 812
            C+ +      +F PC   A +  D+  H   +C A  S         +C+N+ C     C +G    +           CEI  + +   P  +PCQ      G C +  G+  + C C  G +G RCE+  SL      SF    S+VE++   +   +  I I+F T   Q G+LLY    Q + V  +   IR                          SYD+G+  V T+ S+  +  GK        YH+  LL ++     R D  I  +    G+   L ++  L+I GV P D       + +   S  F GCIKE+ +N K   L    +   +R +    C         + C  + CK+EG C S+  G  + C CK  FTG  CQ R
Sbjct:  402 CEKKIPFCKKEFNPCMN-AGKCVDHFTHYTCECKAGFSGANCTKNIDDCLNHMCQNGGTCEDGIDSYTCKCPQEFGGKFCEIEPMVAQLYPQTSPCQQHDCKHGICFQPPGSNNYICKCAPGYSGKRCEYLTSL------SFIHNTSYVEME-ALNVKPKANITIIFAT-EQQNGVLLYTGDNQHLAVELFRGRIRV-------------------------SYDVGNYPVSTMFSYELLSDGK--------YHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGV-PEDVGHKSLKQWHLRNSTSFKGCIKEVYVNDK---LSDFLQAAKVRHKVTPGCSLYQDEQPTDPCVDHKCKHEGECRSKNDGTSYECICKPHFTGHFCQIR 754          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000003115 (pep:novel supercontig:LSalAtl2s:LSalAtl2s174:638829:676917:-1 gene:EMLSAG00000003115 transcript:EMLSAT00000003115 description:"maker-LSalAtl2s174-augustus-gene-6.13")

HSP 1 Score: 66.2402 bits (160), Expect = 3.180e-11
Identity = 70/240 (29.17%), Postives = 100/240 (41.67%), Query Frame = 0
Query:  575 SFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLK-SHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMY--KVRKNAGTSIGFVGCIKELKINKKTISLQSM-REPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKR-DFTGKSC 809
            SFT Q+S +       S     I + FRT N   G+LL+       N  N  + +  F  L              H+    DLGSG V ++ S   +  G W H+      + G ++++  D     T G    L+L G+L++GGVD  D P M             GFVGC+++L IN  +I + S   +  V   R       + C   PC + G C      FLC C R  FTG +C
Sbjct:  487 SFTTQESFLVSFTSWKSTISGSISLKFRT-NELNGILLF-------NGGNPHKDYFAFEILDG------------HLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKIDE-DSESFKTPGEANQLDLVGNLFVGGVDYMD-PYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYAHQQDVGSIRSSCHKMPDQCHDRPCNHGGKCSEGWNRFLCDCSRTSFTGATC 704          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000000551 (pep:novel supercontig:LSalAtl2s:LSalAtl2s108:383112:401265:1 gene:EMLSAG00000000551 transcript:EMLSAT00000000551 description:"maker-LSalAtl2s108-augustus-gene-4.10")

HSP 1 Score: 66.2402 bits (160), Expect = 3.517e-11
Identity = 68/256 (26.56%), Postives = 106/256 (41.41%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGS-------KGCPFGGSCVRTG--GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYS-EKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKE-IVVADFKPC 477
            PVCG D  TY NEC ++   CT+   +    D  C+              K CPF  + +     G++ + Y ++C  E   C +    L++ S   C          +D +Y  E S        ECP     E  PVCG+D +TY + C L++ +C  K GN++ + +PG C     + P     +  +     ++     CS T +E           VCG DG TY     L+  +C+  ++ I  A   PC
Sbjct:  249 PVCGIDGRTYTNECYMMATSCTENELIIKRHDGVCKNNSNLRKLRLASIIKECPFACNLIYQPVCGSDGQTYANKCQFESKNCLTSTKTLSIVSQGPC----------DDPIYEFEPSIAEEDPILECPKFCTFEFIPVCGSDGVTYSNKCHLQSQSC--KSGNNIEMDHPGQC-----DNPFEAVITEEEEEEENDDKCPVFCSFTHEE-----------VCGTDGTTYRNLCHLKYQSCQYPEDNIKKASNGPC 476          

HSP 2 Score: 55.0694 bits (131), Expect = 9.636e-8
Identity = 71/313 (22.68%), Postives = 122/313 (38.98%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE---EAHLC-------GSKGCPFGGSCVRTG--GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGA---VCGXDGRTYETEXDLRRHACKM-QKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPE--GTSPNSANTSCEIMK 526
            PVCGSD +TY N+C L    C    N+ +     C+   EA +            CP   S       G +   Y++ CH++  +C+    N+  +S+  C S              +K       CP +CP+    +  P+CG++ +TY + C L+ + C+ +  + +++ Y G C                                     ++ P++ A     VCG DG TY  +  L    C+   K I + D   C+  A  I D   +EE      ++  + N C   E  G+   + ++ CEI++
Sbjct:  376 PVCGSDGVTYSNKCHLQSQSCKSGNNIEMDHPGQCDNPFEAVITEEEEEEENDDKCPVFCSFTHEEVCGTDGTTYRNLCHLKYQSCQYPEDNIKKASNGPCNS-------------KDKLTDDSNVCPVKCPN----DYSPICGSNGLTYVNECQLKLANCNNQNDSKIKISYYGSCL------------------------------------DKCPEFCATKYEPVCGSDGITYVNDCHLLAGNCRHDNKYIFLLDLGECSPKAQIISD--TYEERSIRPNDNSLLQNTCNTGEVCGSDGKTYSSVCEIIE 633          

HSP 3 Score: 54.6842 bits (130), Expect = 1.375e-7
Identity = 67/246 (27.24%), Postives = 98/246 (39.84%), Query Frame = 0
Query:  228 GRINKPVCGSDHITYKNECDLLQ--AGCTKRINLTVISDLPCEEAHLCGSKGC-PFGGSCVRTG-----GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFG-AVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
             RI  PVCGSD  TY+NEC LLQ  + C ++  L       C++     S+ C  F  +   T      G + + Y ++C+M   +C      L +  HD        CK N +     +  +      ECP A N    PVCG+D  TY + C   +  C                +T+      PC     +F  ++ EED    C        +F  +    VCG DG TY  +  L+  +CK
Sbjct:  184 SRIYYPVCGSDGNTYENEC-LLQGLSRCKRKNALVKAFTGDCDKEDKDISESCNSFNATQCPTVYDPVCGIDGRTYTNECYMMATSCTE--NELIIKRHDG------VCKNNSNL----RKLRLASIIKECPFACNLIYQPVCGSDGQTYANKCQFESKNCLTST------------KTLSIVSQGPCDDPIYEFEPSIAEEDPILECP-------KFCTFEFIPVCGSDGVTYSNKCHLQSQSCK 397          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000005353 (pep:novel supercontig:LSalAtl2s:LSalAtl2s289:184980:192687:-1 gene:EMLSAG00000005353 transcript:EMLSAT00000005353 description:"maker-LSalAtl2s289-snap-gene-2.32")

HSP 1 Score: 60.4622 bits (145), Expect = 2.269e-9
Identity = 91/416 (21.88%), Postives = 153/416 (36.78%), Query Frame = 0
Query:  481 ATRIGDYCLHEESDCLALNS--ECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLT----------------DMNTASFTGQ--------DSHVEIK---------IPTDSVK---RMEIEIMFRTFNVQEGLLLYGQSVNVSN------YENTIRTFPFFIPLSSSNNKCHV-----------FKNIK------HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVE-CEE--NSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD 832
             TR G    +   D +   S       +C+CP+G +  +   +C++    ++C  +PC  G  C   +  +SC C     G  CE  L  +                D+     T Q        D   ++K         I  +S+K    + + + F +   + GLL Y   +N  +      YE+    F F   L   N +  V           F  ++      HI  SYD     ++L    +++        A R     + +    +  +G+ R   ++ +L G L +GG+         K R        F GCIK + I++  + + S      +   G V  C E  N C  NPCKN G+C      + C C  D+ GK+C  R +        +N+ + + KD
Sbjct: 1074 VTRFGKQFDYISDDSIMFRSIEPITTYKCVCPKGYTGMTTRYTCDVPI--NMCYSSPCLNGAKCVSKENDYSCICAPNYVGKNCELSLLTSSCTDNQDLCKLPSRCVDLQKGGVTCQNCSRNYYHDQFCQLKSRSFSPGTYIAFNSIKARNSLNLTLEFAS-QSRRGLLFYNGRLNGEHDFMSISYEDAYLKFSF--SLGDKNQELIVNRVKEGFSDGHFHQVELIIENEHITLSYDFCDKKLSLNHQSRLDSKL---KCANR----TIFQHSECNSYLGNCR---KSFDLSGPLLLGGIPDGKDEKRIKTR-------SFSGCIKNVAIDQFELDMNSY-----VHDNGSVSGCPEKRNFCMSNPCKNGGICSEGWGSYQCKCHSDWDGKNCNHRVSKVYGFTSKRNSEITYKKD 1462          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000003435 (pep:novel supercontig:LSalAtl2s:LSalAtl2s188:581193:593418:1 gene:EMLSAG00000003435 transcript:EMLSAT00000003435 description:"snap_masked-LSalAtl2s188-processed-gene-6.23")

HSP 1 Score: 57.7658 bits (138), Expect = 1.612e-8
Identity = 32/74 (43.24%), Postives = 42/74 (56.76%), Query Frame = 0
Query:  774 EENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGT 847
            +++ C  +PC+N   C S +  + C C R F G +C+    D+CDPNPCQN GVCH    GF C C     YGT
Sbjct:  685 KKDECGSSPCQNGASCKSMIGTYSCQCLRGFQGHNCEFNI-DECDPNPCQNEGVCHDLIDGFQCTCP----YGT 753          
BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Match: EMLSAP00000012352 (pep:novel supercontig:LSalAtl2s:LSalAtl2s903:176857:182009:1 gene:EMLSAG00000012352 transcript:EMLSAT00000012352 description:"maker-LSalAtl2s903-augustus-gene-1.45")

HSP 1 Score: 54.6842 bits (130), Expect = 1.628e-8
Identity = 34/106 (32.08%), Postives = 53/106 (50.00%), Query Frame = 0
Query:  653 FSYDLGSGAVTL--KSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
             SY LG    TL  KS  +I   KW  L   R  + GL+++++   + G +      LN  G +W+GG +P    L ++  K      GF GCI+++KI +K + L
Sbjct:   90 LSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGGKEPLPPGLPHQYYK------GFKGCIRKVKIFRKRLDL 189          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 136.732 bits (343), Expect = 3.412e-31
Identity = 111/385 (28.83%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    FSC C +G  G+ CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  +          H+ F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL   L++GG+       +  V       IG  GCI+ L IN + + L   +   V++  G+ EC ++ CS NPC    LC +   G FLC C     G +C   +N  C PNPC  +  CH+  RG   C C      G++C+    +    +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1215 CDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCE------KVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDG---------HVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQ---VATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQR-AVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNP-CQPNPCHGSAPCHVLSRGGAKCACPLGR-SGSFCE-TVLENAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1558          

HSP 2 Score: 124.02 bits (310), Expect = 2.406e-27
Identity = 119/397 (29.97%), Postives = 172/397 (43.32%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            CR + C FGA+C V  K+G + C+C+ + CSG  + PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +T      C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +     G C   +         +   C +  C   G V +EDS    CVC  +C+   + P      VCG DG TY TE  L++  C+ ++E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CRGAQCAFGATCTV--KNGKAVCECQRV-CSGGYD-PVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDR---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGETV-----CTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQ--VQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSP------VCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-RGPT-LAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPVTG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.5153 bits (236), Expect = 1.429e-18
Identity = 86/317 (27.13%), Postives = 122/317 (38.49%), Query Frame = 0
Query:  201 NCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE-EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSP 515
             C+F + C+  ++ G  +C C  + C G   +PVC  D  TY N+C   QA C ++  +      PC++    C    C FG +C                                                    + K+GK++C C    S G    DPVCG+D +TY SVC L +  C+   G ++RV   G C          CG+  C FG++CE E   CVC   C E  Q        VCG DG TY +E +L  HAC  Q  + VA    C      +  +            + C   +C+CP    P
Sbjct:  293 TCQFNSVCL--SRRGRPHCSCDRVTCDGAY-RPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATC----------------------------------------------------TVKNGKAVCECQRVCSGGY---DPVCGSDGVTYGSVCELESMACT--LGREIRVARRGPCDR--------CGQ--CRFGSLCEVETGRCVCPSECVESAQ-------PVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTF-----------GAVCSAGQCVCPRCEHP 521          

HSP 4 Score: 93.9745 bits (232), Expect = 4.569e-18
Identity = 64/233 (27.47%), Postives = 109/233 (46.78%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  +   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I              +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1710 NPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGR-TYIEY---LNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1934          

HSP 5 Score: 90.5077 bits (223), Expect = 5.254e-17
Identity = 83/296 (28.04%), Postives = 127/296 (42.91%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C PNPC G   C     G   C C  G +GS CE  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L++GG      P   K+ + A  + GF G I+ + +    +  Q      V+R   +     + C+    NPC N G CI +   + C C   F+G  C++
Sbjct: 1474 CQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGA-----PDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEH----VLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEK 1740          

HSP 6 Score: 86.2705 bits (212), Expect = 1.030e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCV------CSVTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC ++  ++       C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +            C++++  P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC R G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQ-EHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGETVCTFGAVCSAGQCVCPRCEH 520          

HSP 7 Score: 59.3066 bits (142), Expect = 1.652e-7
Identity = 81/303 (26.73%), Postives = 112/303 (36.96%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISD-----------LPCEEAHLC-----------------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            + D  S D  C C    C   +  PVCGSD +TY  EC L +A C  R  L V +            LP    H                   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +      C       P+ C +  C+FGA C E++    CVC ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I +    PC
Sbjct:  588 VWDEDSEDGPCVCD-FSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPVTGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQALGHLGCEA-DPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTC------PEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPC 864          

HSP 8 Score: 56.9954 bits (136), Expect = 8.887e-7
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+ +A  G + C C  + C    +  VCGSD +TY NEC L    C +R+++++ S  PC E+
Sbjct:  796 CVEMHCEFGASCVEEA--GFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRES 867          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|399021|sp|P25304.2|AGRIN_RAT (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 127.872 bits (320), Expect = 1.525e-28
Identity = 109/385 (28.31%), Postives = 161/385 (41.82%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGT--FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFV-CVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEAL---MELEVSFLKKDPGSL 907
            CD  PC  GGTC + D    F+C C +G  GS CE       +   S      H  +  PT  +   + + + FR     EGLLLY  +    +          F+ L+  + +         + F +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL  +L++GG+ P +   M   R + G  +G  GCI+ L IN + + L   +    ++  G+ EC ++ C  NPC    LC +   G FLC C     G +C   ++  C PNPC     C +   G   C C      GT+C     +T   +P           FN     +L K L   E  L   M LE+ FL + P  L
Sbjct: 1224 CDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCE------KVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALET-EGLLLYNGNARGKD----------FLALALLDGR---------VQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGI-PEEQVAMVLDRTSVG--VGLKGCIRMLDINNQQLELSDWQR-AAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSP-CQPNPCHGAAPCRVLSSGGAKCECPLGR-SGTFCQ-TVLETAGSRPFLAD-------FNGFSYLEL-KGLHTFERDLGEKMALEMVFLARGPSGL 1567          

HSP 2 Score: 121.324 bits (303), Expect = 1.638e-26
Identity = 119/397 (29.97%), Postives = 171/397 (43.07%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPEC--PSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIK-------YEKPIPCGKSSC-DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA C V  K+G + C+C+ + CSG I  PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C V TG                G++   Y S+C + + AC  +++ L ++S  +C +C +     + C +        C CP C  P  G     PVCG+D +TY S C LR + C ++    +   + G C   +         +   C +  C   G + +EDS    CVC  +C+   + P      VCG DG TY TE DL++  C+ Q+E+ VA    C RG T +          C      C  +     +G       ++C     GS          GTCD   G   C CR G+ G RC+
Sbjct:  363 CHGVQCAFGAVCTV--KNGKAECECQRV-CSG-IYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDP---CGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQIS-LYVASAGHCQTCGE-----KVCTFGAVCSAGQCVCPRCEHPPPG-----PVCGSDGVTYLSACELREAACQQQV--QIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSP------VCGSDGVTYGTECDLKKARCESQQELYVAAQGAC-RGPT-LAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSY--------SGTCDPATG--QCSCRPGVGGLRCD 719          

HSP 3 Score: 95.9005 bits (237), Expect = 1.073e-18
Identity = 65/233 (27.90%), Postives = 111/233 (47.64%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSN-YENTIRT-----FPFFIPLSSSNNKCHVFKNIK-HIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            NPC  GG+C   + T+ C C  G +G  CE  L   S+ D+ T +F G+ +++E     ++V   E+          +   L+ +    SN +E ++RT        +I  ++          +  H+  SYDLGS  V L+S  K+   +W  + A R H++G L++     V GS+      L+  G LW+GG+     P+   + K  GT  GFVGC++++ +  + + L
Sbjct: 1719 NPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGR-TYIEY---LNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL--QKLPVGQALPKAYGT--GFVGCLRDVVVGHRQLHL 1943          

HSP 4 Score: 95.1301 bits (235), Expect = 1.877e-18
Identity = 90/320 (28.12%), Postives = 137/320 (42.81%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCS---GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            C CP    P     +C   K  S C PNPC G   C     G   C C  G +G+ C+  L         A F G  S++E+K       D  ++M +E++F       GLLLY GQ  +             F+ L+  N         +H+ F YDLG GA  ++S   I  G W  +  +R  + G L++    +V+G +  S +     LNL+  L+IGG      P   K+ + A  S GF G I+ + +    +  Q      V+R   +    ++ C+   GNPC N G C+ +   + C C   F+G  C++
Sbjct: 1465 CQCP----PGRFGPTCADEK--SPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNG-FSYLELKGLHTFERDLGEKMALEMVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALHN---------RHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGA-----PDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEH----VLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEK 1749          

HSP 5 Score: 85.5001 bits (210), Expect = 1.671e-15
Identity = 113/471 (23.99%), Postives = 176/471 (37.37%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAK-SGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTCDEHDGTFSCYCRSG-LTGSRCEH 564
            CR   C FGA C    +  G + C CK   C   +  PVCGSD  TY NEC+L +A C ++  + ++   PC     C +  C FG +CV +                       G++   Y S+C +   AC S+  ++    +  C  C       +  C+ N    + E   +     PE CP+    +  P+CG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D +   +        C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC   G  R G  C  E   C+   SECV + + +C  G+  ++  + CE+            +     CQ   TC E   TF   C +G     RCEH
Sbjct:   67 CRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATV-APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQ-EHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLR-----PENCPA----QHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVC-PSECVESAQPVC--GSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGEKVCTFGAVCSAGQCVCPRCEH 520          

HSP 6 Score: 58.9214 bits (141), Expect = 1.877e-7
Identity = 82/308 (26.62%), Postives = 111/308 (36.04%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE----------------------------EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVC-SVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I D  S D  C C    C      PVCGSD +TY  ECDL +A C  +  L V +   C                              A   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G S C+   C   G   +D     + +T   +CS R      K G   D +V     C       P+ C +  C+FGA C E +    C+C ++TC      P+  +  VCG DG TY  E  L+  AC+ + +I      PC    T
Sbjct:  588 IWDEDSEDGPCVCD-FSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHC-----NPHGSYSGTCDPATGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GHSGCTPCSCDPRGAVRDD----CEQMT--GLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPM-TPVTCVEIHCEFGASCVEKAGFAQCICPTLTC------PEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVT 869          

HSP 7 Score: 56.9954 bits (136), Expect = 8.968e-7
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA 270
            C   +C FGASC+   K+G + C C  + C    +  VCGSD +TY NEC L    C +R++++  S  PC+E+
Sbjct:  796 CVEIHCEFGASCV--EKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQES 867          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|2494281|sp|Q90404.1|AGRIN_DIPOM (RecName: Full=Agrin)

HSP 1 Score: 125.561 bits (314), Expect = 6.412e-28
Identity = 130/488 (26.64%), Postives = 212/488 (43.44%), Query Frame = 0
Query:  525 MKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMEL--EVSFLKKDPGSL---------GRLKISS--TKTGLLKLQLSDSQGALTEWITSP-PFFVSDNRPHNIIL---NIYRGELDLDVDGLXA----------FTAVNIDEXFHILG 982
            +K+   CD  PC  GGTC++   +++C C +G  G+ CE  +         F G+ S++  K    +   + I + FR  N+ +GL L         +    +   F+ P           +    + F++ +  G +T K+   I+ G W H+V  R  + G+L ++    +IG +      LNL   L++GG   ++  L   V +    + G  GCI+ L +N     LQ  R   V+   G+ EC  N CS NPCKN G C + + + F C    +F+G +C  + N  CDPNPC  +  C +  + G  C C  G E  G  C+ + ++   DQ   G A  P+  FN     ++        + L +L  EV FL KDP  +         GR    S   + G L+ +    +GA      +P P  V      N++    N  +G + ++ D L +           TA+N+ E F++ G
Sbjct:  606 VKVHRPCDSQPCLHGGTCEDDGVSYTCSCPAGRGGAVCERTIVYF---IPEFGGR-SYLAFKT-MKAYYTVRISMEFRASNL-DGLPLV-------QWTEKGKGLHFYRP----------SEGYVELRFNHGVWDGVITSKT--LIKPGNWHHVVGNRNRRSGMLSVDGEPHLIGESPPGTDGLNLDTDLFLGGTPEDEMTL---VTERTTATKGLQGCIRLLDVNNLIYDLQE-RSNDVLYGSGVGECGNNPCSPNPCKNRGKCHMKEAEMFHCESVGEFSGPTCADKHN-PCDPNPCHQSANCMVLPEGGSKCECPMGRE--GELCE-RVSEAEQDQ---GKAFIPE--FNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYNGQKTDGRGDFVSLNLRDGYLEFKYDLGKGAAVLRSKAPIPLNV-----WNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGG 1050          

HSP 2 Score: 90.5077 bits (223), Expect = 4.857e-17
Identity = 66/245 (26.94%), Postives = 112/245 (45.71%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQ---DSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDP----NDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL--QSMREPLVI 765
            NPCQ GG C      + C C+ G +G +CE  L   S +   + +F G+   + H  +     +V+    E+  +T    +GL+L+ G+    S+Y          I L+  +           +  +YDLGS  VTL+S   +   +W  + A R H  G L++     V GS+  +   L+  G LW+GG++     N  P  Y        S GF+GCIK++ I+++ + L   ++  P ++
Sbjct: 1106 NPCQNGGVCSPRLREYDCMCQRGFSGPQCEKALEEKSASGSESVAFNGRTFIEYHNTVTRSEKAVQVNYFEMSIKT-EATKGLILWSGKIAEKSDY----------IALAVVDG---------FVQMTYDLGSKPVTLRSTIPVNTNQWVRIKANRIHGYGTLQVGNEAPVTGSSPFAATQLDTDGALWLGGIEKLAPGNRLPKAY--------STGFIGCIKDVVIDRQELQLVEDALNNPTIL 1322          

HSP 3 Score: 87.8113 bits (216), Expect = 3.261e-16
Identity = 73/296 (24.66%), Postives = 129/296 (43.58%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQD---SHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL---QSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            CDPNPC     C    +G   C C  G  G  CE           +F  +    S++E+       +D ++++ +E++F   +    +   GQ  +             F+ L+  +          ++ F YDLG GA  L+S   I    W  +  +R  + GL+++ + + V G +  S +     LNL+   ++GG      P   K  + AG   GF G I++L +  K+I L   +++R  + I       C +     NPC+N G+C  +++ + C C+R F+G  C++
Sbjct:  869 CDPNPCHQSANCMVLPEGGSKCECPMGREGELCERVSEAEQDQGKAFIPEFNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYNGQKTDGRGD---------FVSLNLRDG---------YLEFKYDLGKGAAVLRSKAPIPLNVWNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGGA-----PDFNKFARAAGIISGFTGAIQKLSL--KSIPLLKKENIRNAMEISNFRWHACTKTR---NPCQNGGVCSPRLREYDCMCQRGFSGPQCEK 1136          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|460018313|sp|P31696.3|AGRIN_CHICK (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor)

HSP 1 Score: 118.242 bits (295), Expect = 1.490e-25
Identity = 107/380 (28.16%), Postives = 173/380 (45.53%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD +PC  GGTC++    F+C C +G  G+ CE  +        SF G+ S++  K+   +   + I + FR   +  GLLLY       N +N  +    FI L+              +   ++ GSG   + S  ++E GKW  LV  R  + G+L ++  + V G +      LNL   L++GG  P D   M  V +    ++G  G I+ L +N +   L+  +   V+   G+ EC  + C  NPC +   C + + + F C C   +TG +C   RN  CDP PC  +  C +  + G +C C  G E  G +C+ +  +  +  P       P+ +  + L  +  ++L LT    M +EV FL K P  +
Sbjct: 1351 CDSHPCLHGGTCEDDGREFTCRCPAGKGGAVCEKPIRYF---IPSFGGK-SYLAFKM-MKAYHTVRIAMEFRATELS-GLLLY-------NGQNRGKD---FISLALVGG---------FVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVD-GEHVSGESPTGTDGLNLDTDLFVGGA-PED--QMAVVAERTAATVGLKGSIRLLDVNNQMYDLRE-KGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNP-CDPTPCHISATCLVLPEGGAMCACPMGRE--GEFCE-RVTEQDHTMPFL-----PEFNGFSYLELNGLQTLFLTCRQ-MSMEVVFLAKSPSGM 1690          

HSP 2 Score: 107.457 bits (267), Expect = 3.552e-22
Identity = 111/398 (27.89%), Postives = 164/398 (41.21%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDP---VCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE----------EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C+V  K+ +  C+C+ + C GR + PVCGSD+ TY N C+L    C  +  + V    PC+    CG   C FG  C   TG                G +   Y S+C + + AC ++  N+ +++  +C SC  T C +   C+  +      C CP C      E+ P   VCGTD +TYD+ C LRA++C +++   + V   G C           G  S      C           ED  CVC  TC    + P      VCG D  TY  E +L++  C+ ++ + V     C    T      +     C      C  +      G       ++C+    GS         GGTCD   G   C C+ G+ G +C+
Sbjct:  470 CLSVECTFGATCVV--KNREPVCECQQV-CQGRYD-PVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDR---CGK--CQFGAICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRAC-TQQKNILVAAQGDCKSCGTTVCSFGSTCVGGQ------CVCPRC------EQQPLAQVCGTDGLTYDNRCELRAASCQQQK--SIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRSP------VCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTTTPPPLPV---VHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSY--------GGTCDPATG--QCSCKPGVGGLKCD 824          

HSP 3 Score: 99.7525 bits (247), Expect = 7.338e-20
Identity = 88/306 (28.76%), Postives = 125/306 (40.85%), Query Frame = 0
Query:  232 KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCV--------------------------RTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCE-EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICP 510
            +PVCG D +TY +EC + + G  + + +  +    C+    C  + C F   C+                          RT  N+ +  K++CH +            + +   C S    C +   C+   K+ + +C   EC        DPVCG+DN TY + C L A  C  KR  ++RVK+ G C          CGK  C FGA+CE E   CVC   C    Q        VCG DG TY +E +L   AC  QK I+VA    C    T +            +  S CV  +C+CP
Sbjct:  356 EPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDE-CKFNAVCLKRWHARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVE--CTFGATCVV--KNREPVC---ECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKR--EIRVKHKGPCDR--------CGK--CQFGAICEAETGRCVCPTECVPSSQ-------PVCGTDGNTYGSECELHVRACTQQKNILVAAQGDCKSCGTTV-----------CSFGSTCVGGQCVCP 623          

HSP 4 Score: 98.9821 bits (245), Expect = 1.131e-19
Identity = 129/504 (25.60%), Postives = 197/504 (39.09%), Query Frame = 0
Query:  139 FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVI--------------RKLLETEASS------GSNVEGCRHSNCRFGASC-IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCK-WNEDCLYSEKSGK-SLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG-----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECV-NNRCICPEGTSPNSANTSCEI 524
            FG P  C   +S G  R+FF   V+ + +  +P+    L+ +  L+R+               RKLL  + +S       +  + CR   C FGA C         + C CK   C   +  PVCGSD+ TY NEC+L +A C ++  + VIS  PC     C    C FG +CVR+                       G++ K Y+S+C +   AC+ +  N+       C  C       N  C  + ++ +  L S PE CPS    + +PVCG D +TY S C +              R+  C  +       K+  VC   ++     C + +CD  +  VC  DS    +        C ++   P  ++G                                          VCG D RTY    +L   AC +++EI V    PC R G  + G  C  E   C+   +ECV +++ +C  GT  N+  + CE+
Sbjct:   92 FGDPLICDNQVSTGDTRIFF---VNPAPQYMWPAHRNELMLNSSLMRITLRNLEEVEHCVEEHRKLLADKPNSYFTQTPPTPRDACRGMLCGFGAVCERSPTDPSQASCVCKKTACP-VVVAPVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTAGCVCPASCSGVAESIVCGSDGKDYRSECDLNKHACDKQ-ENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPS----KREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKDECKFNAVCLK-RWHARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVKNREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQFGAICEAETGRCVC-PTECVPSSQPVC--GTDGNTYGSECEL 582          

HSP 5 Score: 91.6633 bits (226), Expect = 2.064e-17
Identity = 65/260 (25.00%), Postives = 116/260 (44.62%), Query Frame = 0
Query:  533 PNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQ----------DSHVEIKIPT------------DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL--QSMREPLVI 765
            PNPCQ GGTC     ++ C C+ G +G+ CE  +   +  D    +F G+           SH+  +IP              +++    E+  +T   Q  +L  G+ +  S+Y          I L+  +           +   YDLGS  V L+S   I    WTH+ A R  ++G L++     + GS+      L+  G LW+GG++     + +K+ K    S GF+GCI+++ ++++ + L   ++  P ++
Sbjct: 1840 PNPCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSGKGLERSDY----------IALAIVDG---------FVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGME--RLSVAHKLPK--AYSTGFIGCIRDVIVDRQELHLVEDALNNPTIL 2076          

HSP 6 Score: 81.2629 bits (199), Expect = 3.040e-14
Identity = 67/294 (22.79%), Postives = 124/294 (42.18%), Query Frame = 0
Query:  531 CDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCE------HDLS-LTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRT----LNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINK-KTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            CDP PC    TC    +G   C C  G  G  CE      H +  L + N  S+   +    + +   + ++M +E++F   +    +   GQ  +             F+ L+  +          ++ + YDLG GA  L+S   +    W  ++ +R  + G++ +   ++V+G +  S +     LNL+   ++GG      P   K+ + A  S  F G ++ + I     +  Q +R  + I       C +     NPC+N G C  +++ + C C+R F+G  C++
Sbjct: 1607 CDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFL---TCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGD---------FVSLALHDG---------YLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGA-----PDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQKP---NPCQNGGTCSPRLESYECACQRGFSGAHCEK 1871          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|1172451|sp|Q05793.1|PGBM_MOUSE (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor)

HSP 1 Score: 117.472 bits (293), Expect = 2.759e-25
Identity = 94/346 (27.17%), Postives = 159/346 (45.95%), Query Frame = 0
Query:  499 NSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGT-FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY--GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENS-CSGNPCKNEGLCISQVQ-GFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            +SE  +  C+CP G +  + N         SL     C      +  DG  ++C C  G +G RCE  +++T   T S +G  S++ +   T++   + +++ F+      G+LL+  G+S  V +          F+ L+             H+ F Y+LGSG   L+SH  +  G+W  + A+R ++DG L ++    V+ S+ G  + LNL   L++GGV+P+      ++      S  F GC+ E+ +N K + L        +  +G+ +C ++S C   PC+N   C+   +  F C C+  F G  C+   N      PC N G C    RG  C+C
Sbjct: 3179 DSESSSYTCVCPAGFTAAAVNIRKPCTATPSLWADATC-----VNRPDGRGYTCRCHLGRSGVRCEEGVTVT---TPSMSGAGSYLALPALTNTHHELRLDVEFKPLE-PNGILLFSGGKSGPVED----------FVSLAMVGG---------HLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGVEPS-----VQLSPATNMSAHFHGCVGEVSVNGKRLDLTYS----FLGSQGVGQCYDSSPCERQPCRNGATCMPAGEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTC----RGARCLC 3483          

HSP 2 Score: 84.3445 bits (207), Expect = 4.160e-15
Identity = 79/301 (26.25%), Postives = 130/301 (43.19%), Query Frame = 0
Query:  544 EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT--DSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCI-SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD-RGFVCVC 839
            E  GT+ C   +     +    L + +     FT Q  +  + +PT  D+ ++ EI+I FR  +  +G+LLY GQ  + +N  N     P FI       +           F +D GSG  T++    +  G++  +   R    G L +     V G+++G  + L+L   L++GG     +P  Y     AG S GFVGC++EL+I  + I    +     +   G+  C   +C   PC+N G C  S+   + C C   FT  +   R+     P+   +    +  D RG+ C C
Sbjct: 2955 EDAGTYVCTATNRQGKVKAFAYLQVPERVIPYFT-QTPYSFLPLPTIKDAYRKFEIKITFRP-DSADGMLLYNGQKRSPTNLANRQ---PDFISFGLVGGRPE---------FRFDAGSGMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGG-----YP-DYGAIPKAGLSSGFVGCVRELRIQGEEIVFHDVN----LTTHGISHCP--TCQDRPCQNGGQCQDSESSSYTCVCPAGFTAAAVNIRKPCTATPSLWADATCVNRPDGRGYTCRC 3229          

HSP 3 Score: 64.6994 bits (156), Expect = 3.813e-9
Identity = 67/264 (25.38%), Postives = 106/264 (40.15%), Query Frame = 0
Query:  535 PCQGGGTCDEHDGTFSCYCRSGLTGSRCE----HDLSLTDMNTASFTGQDSHVE----------IKIPTDSVKRM------EIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSC 778
            PC  GGTC        C C  G +G RC+    + +  +D +     G D+  +          + +P +S  R        IE   RT +  +GLLL+   V  ++          FI L   +          H++FSY LGSG   L S   I  G+W  + A R  Q G ++++  D V G + G    +N +  ++IGG      P +  + +   +S G  GCIK L ++          +PL ++ R         C
Sbjct: 3469 PCLNGGTCR----GARCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEVRT-STADGLLLWQGVVREASRSKD------FISLGLQDG---------HLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSIQVDGEDLVTGRSPGPNVAVNTKDIIYIGGA-----PDVATLTRGKFSS-GITGCIKNLVLHTARPGAPP-PQPLDLQHRAQAGANTRPC 3705          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|460018339|sp|O00468.5|AGRIN_HUMAN (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor)

HSP 1 Score: 112.849 bits (281), Expect = 6.212e-24
Identity = 111/407 (27.27%), Postives = 161/407 (39.56%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSC-VRTG----------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSC-------------------DFGAVCEEDSY---CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C    C FGA+C V  K+G + C+C  +     +  PVCGSD +TY + C+L    CT    + V    PC+    CG   C FG  C   TG                G++   Y S+C + + AC  +++ L ++S   C +C      +  C +        C CP C    +    PVCG+D +TY S C LR + C ++               I+  +  PC ++ C                     G + +EDS    CVC  +C+     P      VCG DG TY TE +L++  C+ Q+ + VA    C RG T            C      C  +      G       ++C+    GS         GGTCD   G   C CR G+ G RC+ 
Sbjct:  468 CLGVQCAFGATCAV--KNGQAACEC--LQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDR---CGQ--CRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQIS-LHVASAGPCETCG-----DAVCAFGAVCSAGQCVCPRCE---HPPPGPVCGSDGVTYGSACELREAACLQQT-------------QIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSP------VCGSDGVTYSTECELKKARCESQRGLYVAAQGAC-RGPT-FAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSY--------GGTCDPATG--QCSCRPGVGGLRCDR 825          

HSP 2 Score: 112.464 bits (280), Expect = 8.878e-24
Identity = 110/390 (28.21%), Postives = 160/390 (41.03%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEH--DGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG-FLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI-KDRGFVCVCS-GSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFN-------NTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            CD  PC  GGTC +    G F+C C +G  G+ CE  L        +F G+     +  PT  +   + + + FR    Q GLLLY  +    +          F+ L+  + +         +   +D GSG   L S   +E G+W  L   R+ + G L ++    V+G +      LNL   L++GGV P D   +   R   G   G  GCI+ L +N + + L  +      R  G+ EC ++ C  NPC     C +   G F C C     G +C   ++  C PNPC     C +  + G  C C  G E  GT+C      T + Q   G   A    F+       +T A DL +         M LEV FL + P  L
Sbjct: 1333 CDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLG---APVPAFEGRSF---LAFPTLRAYHTLRLALEFRALEPQ-GLLLYNGNARGKD----------FLALALLDGR---------VQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGV-PEDQAAVALERTFVGA--GLRGCIRLLDVNNQRLEL-GIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSP-CQPNPCHGAAPCRVLPEGGAQCECPLGRE--GTFC-----QTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEK--------MALEVVFLARGPSGL 1676          

HSP 3 Score: 100.908 bits (250), Expect = 3.442e-20
Identity = 95/314 (30.25%), Postives = 133/314 (42.36%), Query Frame = 0
Query:  202 CRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSC-VRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC 495
            CRF A C+  ++ G   C C  + C G   +PVC  D  TY ++C   QA C ++  +      PC++A   C    C FG +C V+ G                  G++   Y S C +E  AC +    + ++    C  C Q C++   C    ++G+ +C     PS       PVCG+D  TY S C L    C+ +    + V   G C T        CG + C FGAVC     CVC    + E   P    G VCG DG TY +  +LR  AC  Q +I  A   PC +     G     E+ DC
Sbjct:  399 CRFNAVCL--SRRGRPRCSCDRVTCDGAY-RPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATAC-TLGREIQVARKGPCDRCGQ-CRFGALC--EAETGRCVC-----PSECVALAQPVCGSDGHTYPSECMLHVHACTHQ--ISLHVASAGPCET--------CGDAVCAFGAVCSA-GQCVCP---RCEHPPP----GPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDC 682          

HSP 4 Score: 97.4413 bits (241), Expect = 3.460e-19
Identity = 127/507 (25.05%), Postives = 191/507 (37.67%), Query Frame = 0
Query:  123 LRAERVLKGS-LMRREL------------FGGP--CAVSLSEG-KRVFFLEHVDESLRLKFPS----LLASDKLIRVIRKLLE-----TEASSGSNV--------EGCRHSNCRFGASCIVDAKS-GDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-------SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSL--------------RASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCD--FGAVCEEDSYCVCS------VTCKEEEQFPKYYAG----------------------------------------AVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR-GATRIGDYCLHEESDCLALNSECV 503
            +R  R LKG  L+ RE             FG P  C   +S G  R+FF+      L   +P+    L+ +  L+R+  + LE      E   G++         + CR   C FGA C  +A+  G + C CK   C   +  PVCGSD  TY NEC+L +A C+++  + ++S  PC     C +  C FG +C R+                       G++   Y  +C +   AC +R  N+       C  C       S++C+ N      E     L     CP+     + PVCG D +TY++ C +              R+  C  +       ++  VC + +      C + +CD  +  VC +D     S        C+++   P  + G                                         VCG DG TY +  +L   AC + +EI VA   PC R G  R G  C  E   C+   SECV
Sbjct:   68 VRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYL---WPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCP-SVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRAC-ARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEM----LLRPESCPA----RQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVC-PSECV 560          

HSP 5 Score: 91.6633 bits (226), Expect = 1.942e-17
Identity = 87/328 (26.52%), Postives = 138/328 (42.07%), Query Frame = 0
Query:  499 NSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNT--ASFTGQDSHVEIK----IPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLR----TLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSC---SGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            N E     C CP    P     +C   K  S C PNPC G   C    +G   C C  G  G+ C+            A F G  SH+E++       D  ++M +E++F       GLLLY GQ  +             F+ L+  +         + + F YDLG GA  ++S   +  G WT +  +R  + G L +    +V+G +  S +     LNL+  L++GG      P   K+ + A  S GF G I+ + +  +    Q +    V+R+  +     + C   SG+PC N   C+ +   ++C C   F+G  C++
Sbjct: 1566 NLEAGRFHCQCP----PGRVGPTCADEK--SPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGF-SHLELRGLHTFARDLGEKMALEVVFLARG-PSGLLLYNGQKTDGKGD---------FVSLALRD---------RRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGA-----PDFSKLARAAAVSSGFDGAIQLVSLGGR----QLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEK 1858          

HSP 6 Score: 87.0409 bits (214), Expect = 6.167e-16
Identity = 60/232 (25.86%), Postives = 109/232 (46.98%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDL---SLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFP----FFIPLSSSNNKCHVFKNI--KHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISL 756
            +PC  G +C   +  + C C  G +G  CE  L   S  D++T +F G+     +   T+S    EI +        +   L+ +++  +++E ++RT            ++    +V   I   H+  SY+LGS  V L+S   +   +W  +VA R  ++G L++     V GS+      L+  G LW+GG+   + P+   + K  GT  GFVGC++++ + +  + L
Sbjct: 1828 HPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVP----ETLDSGALHEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLP--ELPVGPALPKAYGT--GFVGCLRDVVVGRHPLHL 2051          

HSP 7 Score: 68.5514 bits (166), Expect = 2.670e-10
Identity = 84/303 (27.72%), Postives = 114/303 (37.62%), Query Frame = 0
Query:  209 IVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTV----------------ISDLPCEE------------AHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN--DVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDS---YCVCSV-TCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            I D  S D  C C    C      PVCGSD +TY  EC+L +A C  +  L V                ++ L C +            A   G  GCP    C     N +  Y   C      C  R   +     D C    +   WN   + ++  G+S C+   C   G   +D     + +T   +CS +      K G   D R   P  C       P  C +  C+FGA C E+S   +CVC + TC      P+  A  VCG DG TY  E  L+  AC+   +I +    PC
Sbjct:  693 IWDEDSEDGPCVCD-FSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQC-----NPHGSYGGTCDPATGQCSCR-PGVGGLRCDRC----EPGFWNFRGIVTD--GRSGCTPCSCDPQGAVRDD----CEQMT--GLCSCKPGVAGPKCGQCPDGRALGPAGCEA-DASAPATCAEMRCEFGARCVEESGSAHCVCPMLTC------PEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPC 969          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|317373536|sp|P98160.4|PGBM_HUMAN (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; AltName: Full=Perlecan; Short=PLC; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor)

HSP 1 Score: 110.923 bits (276), Expect = 3.407e-23
Identity = 95/347 (27.38%), Postives = 163/347 (46.97%), Query Frame = 0
Query:  499 NSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTC-DEHDGT-FSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY--GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENS-CSGNPCKNEGLCISQVQ-GFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            +SE  +  C+CP G +     + CE  +    C P  C    TC +  DG  ++C C  G +G RCE  +++T   T S +G  S++ +   T++   + +++ F+     +G+LL+  G+S  V +          F+ L+             H+ F Y+LGSG   L+S   +  G+W  + A+R ++DG L +     V+ S+ G  + LNL   L++GGV+P+  PL      +A     F GC+ E+ +N K + L        +  +G+ +C ++S C   PC++   C+   +  F C C+  F G  C+   N      PC + G C    +G  C+C
Sbjct: 3861 DSESSSYVCVCPAGFT----GSRCEHSQ-ALHCHPEACGPDATCVNRPDGRGYTCRCHLGRSGLRCEEGVTVT---TPSLSGAGSYLALPALTNTHHELRLDVEFKPL-APDGVLLFSGGKSGPVED----------FVSLAMVGG---------HLEFRYELGSGLAVLRSAEPLALGRWHRVSAERLNKDGSLRVNGGRPVLRSSPGKSQGLNLHTLLYLGGVEPS-VPLSPATNMSA----HFRGCVGEVSVNGKRLDLTYS----FLGSQGIGQCYDSSPCERQPCQHGATCMPAGEYEFQCLCRDGFKGDLCEHEENPCQLREPCLHGGTC----QGTRCLC 4166          

HSP 2 Score: 95.9005 bits (237), Expect = 1.360e-18
Identity = 79/299 (26.42%), Postives = 128/299 (42.81%), Query Frame = 0
Query:  547 GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPT--DSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCI-SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVC--HIKDRGFVCVC 839
            GT+ C   +     +    L + +     FT Q  +  + +PT  D+ ++ EI+I FR  +  +G+LLY GQ     +  N     P FI       +           F +D GSG  T++    +  G +  +   R    G L +     V G+++G  + L+L   L++GG     +P  Y     AG S GF+GC++EL+I  + I    +     +   G+  C   +C   PC+N G C  S+   ++C C   FTG  C+  +   C P  C  +  C      RG+ C C
Sbjct: 3637 GTYVCTATNRQGKVKAFAHLQVPERVVPYFT-QTPYSFLPLPTIKDAYRKFEIKITFRP-DSADGMLLYNGQKRVPGSPTNLANRQPDFISFGLVGGRPE---------FRFDAGSGMATIRHPTPLALGHFHTVTLLRSLTQGSLIVGDLAPVNGTSQGKFQGLDLNEELYLGG-----YP-DYGAIPKAGLSSGFIGCVRELRIQGEEIVFHDLN----LTAHGISHCP--TCRDRPCQNGGQCHDSESSSYVCVCPAGFTGSRCEHSQALHCHPEACGPDATCVNRPDGRGYTCRC 3912          

HSP 3 Score: 62.7734 bits (151), Expect = 1.553e-8
Identity = 69/264 (26.14%), Postives = 104/264 (39.39%), Query Frame = 0
Query:  535 PCQGGGTCDEHDGTFSCYCRSGLTGSRCE----HDLSLTDMNTASFTGQDSHVE----------IKIPTDSVKRM------EIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSC 778
            PC  GGTC    GT  C C  G +G RC+    H ++ +D +     G D+  +          +  P     R        IE+  RT +   GLLL+ Q V V            FI L   +          H++F Y LGSG   L S   I  G+W  + A R  + G ++++  + V G + G    +N +G ++IGG    D   +   R     S G  GC+K L ++          +PL ++ R         C
Sbjct: 4152 PCLHGGTCQ---GT-RCLCLPGFSGPRCQQGSGHGIAESDWHLEGSGGNDAPGQYGAYFHDDGFLAFPGHVFSRSLPEVPETIELEVRT-STASGLLLW-QGVEVGEAGQGKD----FISLGLQDG---------HLVFRYQLGSGEARLVSEDPINDGEWHRVTALREGRRGSIQVDGEELVSGRSPGPNVAVNAKGSVYIGGAP--DVATLTGGR----FSSGITGCVKNLVLHSARPGAPPP-QPLDLQHRAQAGANTRPC 4389          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|221271989|sp|B4F785.1|EGFLA_RAT (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 96.2857 bits (238), Expect = 6.076e-19
Identity = 87/322 (27.02%), Postives = 140/322 (43.48%), Query Frame = 0
Query:  496 LALNSECVNNRCICPEGTSPNSANTS-CEIMKIGSLCDPNPCQGGGTCDEHDG-TFSCYCRSGLTGSRCEHDLSLT----DMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            LA+N + ++ R   P G + N A+   C       +CD   C  GGTC      ++ C C  G  G  CE   +LT      +  S+      +E   P   +   E EI FR  +  +G+LLY       +Y+            +SS +   +     H+ F +D GSG   L+S   +  G+W  L   R  ++G+L++++   V G   G    +     ++IGGV   D      V+KN+G    F G I+++ +N +TI ++      V      VE   + C G PC + G C  + +G+ C C   F G +CQ+
Sbjct:  534 LAVNGKKIDMRP-WPLGKALNGADVGECS----SGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLE---PQHYLSFTEFEITFRP-DSGDGVLLY-------SYD------------TSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYD-----DVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVN-----VENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQK 817          

HSP 2 Score: 86.2705 bits (212), Expect = 7.426e-16
Identity = 73/284 (25.70%), Postives = 118/284 (41.55%), Query Frame = 0
Query:  531 CDPNPCQGGGTC--DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCIS-QVQGFLCHCKRDFTGKSCQ 810
            CD   C     C  D   G   C+C  G  G  C  D+ +       F G  S+V  +   +S +  +I + FR    ++GLLLY G+S +             F+ L+      H         F ++ G+G   + S  KI+ G W  +   R   +GLL+L     V G ++G    +  +  L++GG      P  Y + +  GT+ GF GC++ L +N K I ++       +    + EC    C    C N G C + +   ++C C   F G+ C+
Sbjct:  343 CDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFI---QYPQFFGH-SYVTFEPLKNSYQAFQITLEFRA-EAEDGLLLYCGESEHGRGD---------FMSLALIRRSLH---------FRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-----PSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCE 598          

HSP 3 Score: 67.3958 bits (163), Expect = 4.218e-10
Identity = 57/224 (25.45%), Postives = 95/224 (42.41%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTD----SVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKIN 750
            C   PC  GG+C      + C C  G  G  C+  ++   +    F G+ S++    P      S  R    + F+T   ++GLLL+         ++ +R    FI L   +           ++FSY+LGSG  ++  +G    G+W  + A R  Q G + ++      G + G +R LN+ G L++GG+          +R N     G VGCI    ++
Sbjct:  784 CVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKAIT-EAIEIPQFIGR-SYLTYDNPNILKRVSGSRSNAFMRFKT-TAKDGLLLW-------RGDSPMRPNSDFISLGLRDGA---------LVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKE------IALRTNRQYMRGLVGCISHFTLS 982          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|158705693|sp|A3KN33.1|EGFLA_BOVIN (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 95.5153 bits (236), Expect = 1.023e-18
Identity = 76/287 (26.48%), Postives = 124/287 (43.21%), Query Frame = 0
Query:  530 LCDPNPCQGGGTCDEHDG-TFSCYCRSGLTGSRCEHDLSLT----DMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            +CD   C  GGTC      ++ C C  G  G  CE   +LT      +  S+      +E   P   +  ME E+ FR  + ++G+LLY       +          F+ ++ +           H+ F +D GSG   L+S   +  G W  L   R  ++G+L++++   V G   G    +     ++IGGV   D      V+KN+G    F G I+++ +N +TI ++      V      VE   + C G+PC + G C  + +G+ C C   F G  CQ+
Sbjct:  577 ICDEASCINGGTCMASKADSYICLCPLGFRGRHCEDAFTLTIPQFKESLRSYAATPWPLE---PRHYLSFMEFEVTFRP-DSEDGVLLYSYDTGSKD----------FLSINMAGG---------HVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYD-----DVKKNSGILKPFSGSIQKIILNDRTIHVKHDFTWGVN-----VENAAHPCVGSPCAHGGSCRPRKEGYECDCPLGFEGLHCQK 830          

HSP 2 Score: 84.3445 bits (207), Expect = 3.186e-15
Identity = 73/284 (25.70%), Postives = 122/284 (42.96%), Query Frame = 0
Query:  531 CDPNPCQGGGTC--DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCI-SQVQGFLCHCKRDFTGKSCQ 810
            CD   C     C  D   G   C+C  G  G  C  D+ +       F G  S+V  +   +S +  +I + FR    ++GLLLY G++ +             F+ L+       V +  + + F ++ G+G   + S  KI+ G W  +   R   +GLL+L     V G ++G    +  +  L++GG      P  Y + +  GT+ GF GC++ L +N K + L+       +    + EC    C    C N G C+ S+   ++C C   F G+ C+
Sbjct:  356 CDETVCSADSFCVSDYTWGGSRCHCNLGKGGESCSEDIVI---QYPQFFGH-SYVTFEPLKNSYQAFQITLEFRA-EAEDGLLLYCGENEHGRGD---------FMSLA-------VIR--RSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGA-----PSAYWLVRATGTNRGFQGCVQALTVNGKRLDLRPWPLGKALSGADVGECSSGICDEASCINGGTCMASKADSYICLCPLGFRGRHCE 611          

HSP 3 Score: 67.0106 bits (162), Expect = 5.956e-10
Identity = 57/225 (25.33%), Postives = 98/225 (43.56%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRME-----IEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKIN 750
            C  +PC  GG+C      + C C  G  G  C+  ++   +    F G+ S++    P D +KR+        + F+T   ++GLLL+         ++ +R    FI L   +           ++FSY+LGSG  ++  +G    G+W  + A R  Q G + ++      G + G +R LN+ G L++GG+          +  N     G VGCI    ++
Sbjct:  797 CVGSPCAHGGSCRPRKEGYECDCPLGFEGLHCQKAIT-EAIEIPQFIGR-SYLTYDNP-DILKRVSGSRSNAFMRFKT-TAKDGLLLW-------RGDSPMRPNSDFISLGLRDGA---------LVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMK------EIALHTNRQYMRGLVGCISHFTLS 995          
BLAST of EMLSAG00000004167 vs. SwissProt
Match: gi|121949534|sp|A0A1F4.1|EYS_DROME (RecName: Full=Protein eyes shut; AltName: Full=Protein spacemaker)

HSP 1 Score: 95.5153 bits (236), Expect = 1.612e-18
Identity = 98/384 (25.52%), Postives = 161/384 (41.93%), Query Frame = 0
Query:  477 CTRGATRIGDYCLHEESDCLALNSECVNN-RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGT-FSCYCRSGLTGSRCEHDLSLTDMN-TASFTGQDSHVE--IKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIE-----KGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVD-------PNDFPLMYKVRKNAGTSIGFVGCIK--ELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDP--NPCQNNGVCHIKDRGFVCVC 839
            C     R G  C+  E++ L  N E     +C CP G        +CEI    S+C+ NPCQ GGTC +  G+ + C C  G  G  CEH+L +   + + S  G  S V   + IP +    +  +I+ +T +    L  +GQ    S Y +              ++   V     +I+ +++LG+G   + +   I+           +   R  +   L ++    + G + GS   +++   L++GG +       P+D PL            GF GCI   +LK  + T+ LQ  R    +R RG+ +C    C  + C+++G C+     F C C+  + G  C +  N  CD   N C  +  C     G+ C C
Sbjct: 1266 CLSSPCRNGAACIKIETNDLDENGEKAEKWKCKCPTG----YMGPTCEI----SVCEDNPCQYGGTCVQFPGSGYLCLCPLGKHGHYCEHNLEVALPSFSGSVNGLSSFVAYTVPIPLEYSLELSFKILPQTMSQISLLAFFGQ----SGYHD------------EKSDHLAVSFIQGYIMLTWNLGAGPRRIFTQKPIDFRLDAPRVPYEIKVGRIGRQAWLSVDGKFNITGRSPGSGSRMDVLPILYLGGHEIANFNTLPHDLPLHS----------GFQGCIYDVQLKAGQVTVPLQETRG---VRGRGVGQCGTRECHRHACQHDGACLQHGATFTCICQEGWYGPLCAQPTNP-CDSFNNKCYEDATCVPLVNGYECDC 1611          

HSP 2 Score: 72.4034 bits (176), Expect = 1.751e-11
Identity = 117/506 (23.12%), Postives = 194/506 (38.34%), Query Frame = 0
Query:  536 CQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKI--------PTDSVKRMEIEIMFRTFNVQEGLLLYG--QSVNVSNY-----ENTIRTFPF--------FIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKI----EKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPL--VIRRRGLVECEENSCSGNPCKNEGLCI-----------SQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVC-HIKDRGFVCVCSGSEYYGTYCD--LKTADTPNDQPAY-GSAKAPKTHFNNTLAFDLKKSLGLTEEALMEL--EVSFLKKDPGSLGRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDVDGLXAFTAVNIDEXFHILGFKGKDLQGDRMDV 995
            C  GGTC        C CR    G  CE  + + +   A+F+G DS+V  +I          D+V  M I++  RT     GL++    Q     +Y     +  +  F F           L +  N  H       + FS +      +L  +  +    ++  W  L+  R H         T + I         LN   HL  GG       L+ ++   A +  GF GC+  L+IN +       RE     +   G+ EC   +C  +PC+N   CI            + + + C C   + G +C+      C+ NPCQ  G C      G++C+C   + +G YC+  L+ A      P++ GS     +    T+   L+ SL L+ + L +   ++S L       G+      K+  L +        LT  + + P  +   +P +  L+  R   ++ V  +     +++D  F+I G       G RMDV
Sbjct: 1027 CYNGGTCVTTSEGSRCVCRFDRQGPLCELPIIIRN---AAFSG-DSYVSHRIYKDIGGHESLDAVLPMHIQLKVRT-RATNGLIMLAAAQGTKGGHYMALFLQKGLMQFQFSCGLQTMLLSELETPVNTGHEITIRAELDFSRNYTHCNASLLVNDTLAMSGDQPTWLKLLPPRLH---------TPEAI---------LNTWLHL--GGAPQAPIGLIIEL-PPAQSGSGFTGCLHTLRINGQA------REIFGDALDGFGITECGSLACLSSPCRNGAACIKIETNDLDENGEKAEKWKCKCPTGYMGPTCEI---SVCEDNPCQYGGTCVQFPGSGYLCLCPLGK-HGHYCEHNLEVA-----LPSFSGSVNGLSSFVAYTVPIPLEYSLELSFKILPQTMSQISLL----AFFGQSGYHDEKSDHLAVSFIQGYIMLTWNLGAGPRRIFTQKPIDFRLDAPRVPYEIKVGRIGRQAWLSVDGKFNITGRSPGS--GSRMDV 1485          

HSP 3 Score: 66.6254 bits (161), Expect = 9.407e-10
Identity = 31/86 (36.05%), Postives = 49/86 (56.98%), Query Frame = 0
Query:  771 VECEEN--SCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTA 854
            ++C+ N   C  +PC+N G C+  V  + C C   F+G +C+    D+C  NPCQN G+C  +  G++C C    Y G++C+L  A
Sbjct:  177 IQCQTNWDECWSSPCQNGGTCVDGVAYYNCTCPEGFSGSNCEENV-DECMSNPCQNGGLCRDRTNGYICTCQPG-YLGSHCELDVA 260          

HSP 4 Score: 56.6102 bits (135), Expect = 9.969e-7
Identity = 26/79 (32.91%), Postives = 45/79 (56.96%), Query Frame = 0
Query:  774 EENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLK 852
            E N C+ +PC+N G+C+ ++  + C C   +TG +C+      C  NPCQNN +C +++    C C   +Y+G  C+ +
Sbjct:  300 EINECASSPCQNGGVCVDKLAAYACACPMGYTGINCEEEIL-ICADNPCQNNALCLMEEGVPTCYCV-PDYHGEKCEFQ 376          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: gb|EFA11111.1| (hypothetical protein TcasGA2_TC004709 [Tribolium castaneum])

HSP 1 Score: 181.415 bits (459), Expect = 1.974e-45
Identity = 158/559 (28.26%), Postives = 257/559 (45.97%), Query Frame = 0
Query:  327 TCKWNEDCLYSEKSGKSLCSC-PECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGA----VCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYC--------------------LHEESDCLALNSECVNNRCICPEGTSPNSANTSC----EIMKIGSLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCH-IKDRGFVCVCSGSEYYGTYCDLK 852
            TC +   C+  E+ G ++C C  ECP   + +   VCG+D  TY S C LR   C  ++  D+ V+  G C+   +       +          +DS    S + +      P+YY G+    +    G +Y  E D      K   EI V+   P     TR G+Y                       + SDC+ + S CV+  C C    S +S    C            C  +PC  G TC D    TF+C C +  TGS CE ++     NT +F G+ S+V++K P  +  ++ IE+ F+T +  +GLLLY Q                F+ L+  N           + F Y+LG+G V ++S  KI+ G +  +V KRYH+DG+L+L+  + V G  +G+L+ L+L    ++G + P+++  +Y   +N GT+ GF GCI++LKI ++ I L   R+  V+    + EC ++ CS  PC N+  C +   + + C C   + G+ C++   + C  NPC+    C  +    ++C C      GT C+++
Sbjct:  810 TCYFGATCV--ERGGFAICECHTECPQENDAQV--VCGSDGQTYASACELRQVACRTQK--DIVVQAFGTCKDDMFPSTDWPIRRYTPLQFTQPDDSNSPLSKSTRHLLVPDPRYYYGSSGSIMRPVQGVSYPAETDN-----KAAFEINVSATGPNAIYNTRGGNYAPAYRPTPATVRVITALLGDLCSDNSDCMIMYSHCVSGACTCLPNHSESSDRQECIADPNPTDEYRACSSSPCHHGSTCVDLPAATFTCVCETNFTGSLCETEVIHKQYNTPAFHGR-SYVKLK-PLKAYHKLSIEVEFKTHS-HDGLLLYNQ--------QKPDGLGDFVSLAIVNG---------FVEFKYNLGNGHVLIRSVDKIQLGVFHRVVIKRYHRDGILKLDEGEDVAGQAKGTLKALDLLEDTYVGFL-PSNYTRVY---ENIGTAKGFRGCIRKLKIGRRPIELHVNRDDWVLGAEAVSECGDSPCSAAPCANDAKCALITPETYRCECGPHYRGEFCEKPI-EPCRSNPCKFGSTCTPLSPDAYICECPPGR-TGTRCEME 1331          

HSP 2 Score: 110.923 bits (276), Expect = 1.189e-23
Identity = 91/269 (33.83%), Postives = 125/269 (46.47%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYS---CSQ-TCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            PVCGSD  TY NEC L Q  C  R NL +I    C    + C S  C  G  C                          + + + S+C ++  AC +R T + +S    C     CS+  C+    C+  E+ G + C CP CP+    E +PVCG+D I+Y + C LR   C   R  ++ V Y G C   + +K        CDF +VCE DS     CVC  +C +    P+   G VCG DG TY  E +LR  +CKM++ I+VA
Sbjct:  163 PVCGSDGKTYSNECTLRQEACRARKNLHIIYRGKCSSGINPCMSVRCTLGEECAINKFGIARCQCPPSCEPIMRPVCSKDGRTFPSECELKRTACLTR-TTIEISYSGVCGEKGPCSEYQCQNGATCV--ERFGVAHCECPVCPA----EFEPVCGSDGISYGNECKLRLEACKHPR--NITVLYDGPCNGCETKK--------CDFYSVCERDSATEGRCVCPKSCTD----PELNDGTVCGTDGVTYANECELRMTSCKMKQFILVA 410          

HSP 3 Score: 108.612 bits (270), Expect = 6.133e-23
Identity = 103/344 (29.94%), Postives = 147/344 (42.73%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSG----RINKPVCGSDHITYKNECDLLQAGCTKRINLTV--------ISDLPCEEAHLCG-----SKGCPFGGSC----VRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYS-----CSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEED---SYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPE 511
            CR   C FGA C+V     ++ C C    C        ++PVCGSD I Y+N+C+L +A CT   N+T+         + + C E  +C      +  C  G SC        G++ K Y ++C +  +AC +R  NL +     C S      S  C   E+C  + K G + C   +CP +      PVC  D  T+ S C L+ + C  +    + + Y GVC      +  PC +  C  GA C E    ++C C V   E E         VCG DG +Y  E  LR  ACK  + I V    PC    T+  D+    E D           RC+CP+
Sbjct:   54 CRDKQCGFGARCVVSPDGRNASCVCPD-KCPSYGDHTTSRPVCGSDGIDYRNQCELQKAACTSNTNITIKFLGKCDPCAGVECAEPEVCQLDEHRNPVCRCGDSCPLEFTPVCGSDGKTYSNECTLRQEACRAR-KNLHIIYRGKCSSGINPCMSVRCTLGEECAIN-KFGIARC---QCPPSCEPIMRPVCSKDGRTFPSECELKRTACLTR--TTIEISYSGVC-----GEKGPCSEYQCQNGATCVERFGVAHCECPVCPAEFE--------PVCGSDGISYGNECKLRLEACKHPRNITVLYDGPCNGCETKKCDFYSVCERD------SATEGRCVCPK 370          

HSP 4 Score: 94.7449 bits (234), Expect = 1.156e-18
Identity = 78/290 (26.90%), Postives = 124/290 (42.76%), Query Frame = 0
Query:  531 CDPNPCQGGGTCDE-HDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEEN---------SCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            C  NPC+ G TC       + C C  G TG+RCE +     +    F+G  S++ +       K   IE+ F       GL+LY   +     +        FI L+ +           H+ F ++LGSG   L +   +  GKW      R  ++G+L+L+ +  V G +   L  LNL+   +IG +   D     +V + +G S G+ G I+ + +N K + + S + P          C  N           S NPC N GLC+ ++  F+C C  +  GK C+
Sbjct: 1296 CRSNPCKFGSTCTPLSPDAYICECPPGRTGTRCEMEDKYMGIVNPEFSGS-SYIRLPRLEGVRKTFSIEVYFMP-KAANGLILYNGQLKNGRGD--------FISLNLARG---------HLQFRFNLGSGIANLTTKETVNIGKWHWARIFRDGREGILQLDNSSIVRGYSGTPLTELNLELPFYIGSLSEWD-----EVHRLSGASKGYKGVIQRILLNGKPLPI-SAKLPDCSFDSNRNGCAFNVGSYDGLPCPISKNPCLNNGLCVPELDQFICKCPSNTKGKYCE 1560          

HSP 5 Score: 87.4261 bits (215), Expect = 1.925e-16
Identity = 84/295 (28.47%), Postives = 122/295 (41.36%), Query Frame = 0
Query:  234 VCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI------VVADFKPCTRGATRIGDYCLH 490
            VCG+D  TY N C L  A C  R+N  V  D  CE+   C  K C FG  CV +                           G++   Y++QC ++  AC S  TN+T+     C  C+   C   E C   E     +C C + CP     E  PVCG+D  TY + C+LR   C  ++  ++ + Y G C +       PC    C  G  C  + +    C C  +C+   +        VC  DGRT+ +E +L+R AC  +  I      V  +  PC+    + G  C+ 
Sbjct:   15 VCGTDGKTYANRCQLRVASCKARLNTRVKHDGECEQNDPCRDKQCGFGARCVVSPDGRNASCVCPDKCPSYGDHTTSRPVCGSDGIDYRNQCELQKAACTSN-TNITIKFLGKCDPCAGVECAEPEVCQLDEHR-NPVCRCGDSCPL----EFTPVCGSDGKTYSNECTLRQEACRARK--NLHIIYRGKCSS----GINPCMSVRCTLGEECAINKFGIARCQCPPSCEPIMR-------PVCSKDGRTFPSECELKRTACLTRTTIEISYSGVCGEKGPCSEYQCQNGATCVE 290          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_016770916.1 (PREDICTED: agrin-like isoform X4 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.217e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1214 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1655          

HSP 2 Score: 101.293 bits (251), Expect = 1.272e-20
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1726 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 2002          

HSP 3 Score: 92.8189 bits (229), Expect = 5.021e-18
Identity = 92/311 (29.58%), Postives = 132/311 (42.44%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E E   +     VC   G+TY +  +L+R AC  +  I VA
Sbjct:  479 ASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECEPVMR----PVCARGGKTYTSLCELKRQACLTRTNIEVA 771          

HSP 4 Score: 88.1965 bits (217), Expect = 1.289e-16
Identity = 79/315 (25.08%), Postives = 137/315 (43.49%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPE----CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP           +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  694 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECE-PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAECK-TDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 984          

HSP 5 Score: 70.8626 bits (172), Expect = 2.232e-11
Identity = 67/258 (25.97%), Postives = 106/258 (41.09%), Query Frame = 0
Query:  238 DHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEE-EDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
            ++++YK +   ++    K +N    +  PCE+ +      C +G +CV +                     G++N  Y + CH+   +C  R +    +       D C   +  C     C+ S    ++ C C E CPS G+ E   PVCGTD   Y S+C +  + C+K  G ++ + + G C         PCG   C    +C+ D      C C   C  E          VCG DG+TY  E  LR+ AC+
Sbjct:  460 NYMSYKPQRPTIEG---KDLNFASNNAEPCEKTY------CSWGATCVVSENGKPLCQCPTDCPSTSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLN--CSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAACAK--GANITMAFQGKCD--------PCGNVECVEPEICQLDESRQPGCRCGEQCGLE-------FAPVCGSDGKTYSNECSLRQEACR 689          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_006566972.1 (PREDICTED: agrin-like isoform X7 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.397e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct:  996 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1437          

HSP 2 Score: 102.064 bits (253), Expect = 7.572e-21
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1508 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 1784          

HSP 3 Score: 93.9745 bits (232), Expect = 2.047e-18
Identity = 116/431 (26.91%), Postives = 171/431 (39.68%), Query Frame = 0
Query:   93 SSLDYRVSASDVILVGSKSPLLSGEEDEGGLRAERVLKGSL--MRRELFGGPCAVSLSEGKRVFFL---EHVDESLR-----LKF---PSLLAS-DKLIRVIRKLLETEAS----------SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            S+   +V  +D I  G    L +G      +R +R +KG L           CA  +       F+   E +D   R     + F   P  LA+ D+L   +R +                + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E +        VC   G+TY +  +L+R AC  +  I VA
Sbjct:   44 STFVGKVRQADYIFTGKIKDLRNGHVH---VRVKRAIKGELNATLDLTLNDTCARYIRPSYTGIFMARREAIDSDQRRDRIVMHFGPVPLTLANLDRLNAAVRDMSYKPQRPTIEGKDLNFASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECE---PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVA 454          

HSP 4 Score: 88.1965 bits (217), Expect = 1.260e-16
Identity = 80/315 (25.40%), Postives = 140/315 (44.44%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCP-ECPSA---GNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP +C  +     +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  377 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECEP-VMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAEC-KTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 667          

HSP 5 Score: 81.6481 bits (200), Expect = 1.223e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  465 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 775          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_006566973.1 (PREDICTED: agrin-like isoform X8 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.417e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct:  985 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMN-RLKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1426          

HSP 2 Score: 102.064 bits (253), Expect = 7.307e-21
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1497 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 1773          

HSP 3 Score: 94.3597 bits (233), Expect = 1.572e-18
Identity = 115/421 (27.32%), Postives = 170/421 (40.38%), Query Frame = 0
Query:   93 SSLDYRVSASDVILVGSKSPLLSGEEDEGGLRAERVLKGSL--MRRELFGGPCAVSLSEGKRVFFL---EHVDESLR-----LKF---PSLLAS-DKLIRVIRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            S+   +V  +D I  G    L +G      +R +R +KG L           CA  +       F+   E +D   R     + F   P  LA+ D+L   +R +   +    +  E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E +        VC   G+TY +  +L+R AC  +  I VA
Sbjct:   44 STFVGKVRQADYIFTGKIKDLRNGHVH---VRVKRAIKGELNATLDLTLNDTCARYIRPSYTGIFMARREAIDSDQRRDRIVMHFGPVPLTLANLDRLNAAVRDM-SYKPQRPTIEEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECE---PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVA 443          

HSP 4 Score: 88.1965 bits (217), Expect = 1.196e-16
Identity = 80/315 (25.40%), Postives = 140/315 (44.44%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCP-ECPSA---GNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP +C  +     +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  366 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECEP-VMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAEC-KTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 656          

HSP 5 Score: 81.6481 bits (200), Expect = 1.113e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  454 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 764          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_006566971.1 (PREDICTED: agrin-like isoform X6 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.680e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1006 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1447          

HSP 2 Score: 101.679 bits (252), Expect = 8.115e-21
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1518 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 1794          

HSP 3 Score: 90.1225 bits (222), Expect = 3.090e-17
Identity = 90/305 (29.51%), Postives = 128/305 (41.97%), Query Frame = 0
Query:  195 EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E +        VC   G+TY +  +L+R AC  +  I VA
Sbjct:  177 EPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECE---PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVA 464          

HSP 4 Score: 88.1965 bits (217), Expect = 1.251e-16
Identity = 80/315 (25.40%), Postives = 140/315 (44.44%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCP-ECPSA---GNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP +C  +     +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  387 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECEP-VMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAEC-KTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 677          

HSP 5 Score: 81.6481 bits (200), Expect = 1.106e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  475 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 785          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_006566970.1 (PREDICTED: agrin-like isoform X5 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.786e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1017 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMN-RLKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1458          

HSP 2 Score: 101.679 bits (252), Expect = 8.479e-21
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1529 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 1805          

HSP 3 Score: 92.8189 bits (229), Expect = 4.999e-18
Identity = 91/310 (29.35%), Postives = 131/310 (42.26%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E +        VC   G+TY +  +L+R AC  +  I VA
Sbjct:  183 ASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECE---PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVA 475          

HSP 4 Score: 87.8113 bits (216), Expect = 1.484e-16
Identity = 80/315 (25.40%), Postives = 140/315 (44.44%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCP-ECPSA---GNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP +C  +     +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  398 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECEP-VMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAEC-KTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 688          

HSP 5 Score: 81.6481 bits (200), Expect = 1.226e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  486 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 796          

HSP 6 Score: 72.4034 bits (176), Expect = 7.445e-12
Identity = 68/257 (26.46%), Postives = 105/257 (40.86%), Query Frame = 0
Query:  239 HITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEE-EDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
            H++YK +   ++    K +N    +  PCE+ +      C +G +CV +                     G++N  Y + CH+   +C  R +    +       D C   +  C     C+ S    ++ C C E CPS G+ E   PVCGTD   Y S+C +  + C+K  G ++ + + G C         PCG   C    +C+ D      C C   C  E          VCG DG+TY  E  LR+ AC+
Sbjct:  165 HMSYKPQRPTIEG---KDLNFASNNAEPCEKTY------CSWGATCVVSENGKPLCQCPTDCPSTSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLN--CSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAACAK--GANITMAFQGKCD--------PCGNVECVEPEICQLDESRQPGCRCGEQCGLE-------FAPVCGSDGKTYSNECSLRQEACR 393          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_006566966.1 (PREDICTED: agrin-like isoform X1 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.824e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1313 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1754          

HSP 2 Score: 100.908 bits (250), Expect = 1.423e-20
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1825 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 2101          

HSP 3 Score: 92.4337 bits (228), Expect = 6.263e-18
Identity = 92/311 (29.58%), Postives = 132/311 (42.44%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E E   +     VC   G+TY +  +L+R AC  +  I VA
Sbjct:  479 ASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECEPVMR----PVCARGGKTYTSLCELKRQACLTRTNIEVA 771          

HSP 4 Score: 87.8113 bits (216), Expect = 1.809e-16
Identity = 79/315 (25.08%), Postives = 137/315 (43.49%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPE----CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP           +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  694 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECE-PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAECK-TDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 984          

HSP 5 Score: 80.8777 bits (198), Expect = 1.937e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  782 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 1092          

HSP 6 Score: 70.8626 bits (172), Expect = 2.445e-11
Identity = 67/258 (25.97%), Postives = 106/258 (41.09%), Query Frame = 0
Query:  238 DHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEE-EDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
            ++++YK +   ++    K +N    +  PCE+ +      C +G +CV +                     G++N  Y + CH+   +C  R +    +       D C   +  C     C+ S    ++ C C E CPS G+ E   PVCGTD   Y S+C +  + C+K  G ++ + + G C         PCG   C    +C+ D      C C   C  E          VCG DG+TY  E  LR+ AC+
Sbjct:  460 NYMSYKPQRPTIEG---KDLNFASNNAEPCEKTY------CSWGATCVVSENGKPLCQCPTDCPSTSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLN--CSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAACAK--GANITMAFQGKCD--------PCGNVECVEPEICQLDESRQPGCRCGEQCGLE-------FAPVCGSDGKTYSNECSLRQEACR 689          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_016770915.1 (PREDICTED: agrin-like isoform X3 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.831e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1313 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1754          

HSP 2 Score: 101.293 bits (251), Expect = 1.270e-20
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1825 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 2101          

HSP 3 Score: 92.8189 bits (229), Expect = 5.358e-18
Identity = 92/311 (29.58%), Postives = 132/311 (42.44%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E E   +     VC   G+TY +  +L+R AC  +  I VA
Sbjct:  479 ASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECEPVMR----PVCARGGKTYTSLCELKRQACLTRTNIEVA 771          

HSP 4 Score: 87.8113 bits (216), Expect = 1.588e-16
Identity = 79/315 (25.08%), Postives = 137/315 (43.49%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPE----CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP           +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  694 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECE-PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAECK-TDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 984          

HSP 5 Score: 81.2629 bits (199), Expect = 1.805e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  782 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 1092          

HSP 6 Score: 70.8626 bits (172), Expect = 2.167e-11
Identity = 67/258 (25.97%), Postives = 106/258 (41.09%), Query Frame = 0
Query:  238 DHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEE-EDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
            ++++YK +   ++    K +N    +  PCE+ +      C +G +CV +                     G++N  Y + CH+   +C  R +    +       D C   +  C     C+ S    ++ C C E CPS G+ E   PVCGTD   Y S+C +  + C+K  G ++ + + G C         PCG   C    +C+ D      C C   C  E          VCG DG+TY  E  LR+ AC+
Sbjct:  460 NYMSYKPQRPTIEG---KDLNFASNNAEPCEKTY------CSWGATCVVSENGKPLCQCPTDCPSTSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLN--CSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAACAK--GANITMAFQGKCD--------PCGNVECVEPEICQLDESRQPGCRCGEQCGLE-------FAPVCGSDGKTYSNECSLRQEACR 689          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_016770914.1 (PREDICTED: agrin-like isoform X2 [Apis mellifera])

HSP 1 Score: 158.303 bits (399), Expect = 3.916e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+       +  T   + D  +H        V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct: 1302 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYFYTHRAHQE--TITIDRDNLKHG-------VAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1743          

HSP 2 Score: 101.293 bits (251), Expect = 1.328e-20
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1814 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 2090          

HSP 3 Score: 89.7373 bits (221), Expect = 4.210e-17
Identity = 91/306 (29.74%), Postives = 129/306 (42.16%), Query Frame = 0
Query:  195 EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E E   +     VC   G+TY +  +L+R AC  +  I VA
Sbjct:  473 EPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECEPVMR----PVCARGGKTYTSLCELKRQACLTRTNIEVA 760          

HSP 4 Score: 87.8113 bits (216), Expect = 1.565e-16
Identity = 79/315 (25.08%), Postives = 137/315 (43.49%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPE----CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP           +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  683 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECE-PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAECK-TDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQ--ALITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 973          

HSP 5 Score: 80.8777 bits (198), Expect = 1.952e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  771 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 1081          
BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Match: XP_016770917.1 (PREDICTED: agrin-like isoform X9 [Apis mellifera])

HSP 1 Score: 157.532 bits (397), Expect = 5.384e-38
Identity = 142/494 (28.74%), Postives = 218/494 (44.13%), Query Frame = 0
Query:  341 GKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR---TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQF------PKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRI-----GDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIG-------SLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQR 811
            G   C CP  CPS  +    PVCG+D  TYD+ C LR   C  +   DV  +  G CR    +  + P+        + AV         S   K           P+Y+         R ++    + R      K  V A ++P T    R+     GD C  ++ DC   NSEC N  CIC EG +  S    C    +          C   PC    TC D    TF C CR   TG  CE +++  D   ASF G+ S+V +     +  +  +E+ F+T+    G++LY Q  +    +        F+ L+  +          H+ F Y+LG+G V L S  ++    +  + AKRYH+DG+L     + V+G ++G L++L+L    ++G +  N      KV +N GT+ GF+GCI++LKIN+  + L   R+  ++    + EC EN+C+  PC+N   C    +  LC+      G  C+R
Sbjct:  887 GGPHCVCPSSCPS--DIPSVPVCGSDGQTYDNECELRLYAC--RHQADVVTQAFGHCRDDPMVNTDFPVK------RYTAVQYTQPAAAISPLSKSTRHLLVPEPDPRYF------YTHRAHQETITIDR---DNLKHGVAAAYRP-TPATIRVVTALLGDLCT-DDKDCTIPNSECSNGGCICSEGYAETSDRQECFANYVPVTPTEEFRACLSYPCHATSTCIDLPSATFVCICRPNYTGRFCE-EMNKRDYEVASFDGK-SYVRMNR-LKAYHKFSVEVEFKTY-ADNGIILYNQQKSDGTGD--------FVSLAIVDG---------HVQFRYNLGNGPVILTSPERVTMKTFHSVAAKRYHKDGVLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTN----YSKVYENIGTNHGFLGCIRKLKINRIHVDLHVGRDKEILETYRVKECGENACANLPCQNGATCQPIFEEDLCN------GHECRR 1328          

HSP 2 Score: 101.679 bits (252), Expect = 8.829e-21
Identity = 87/309 (28.16%), Postives = 134/309 (43.37%), Query Frame = 0
Query:  504 NNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCE-HDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            N RCICP    P     +CE  +    C   PCQ G TCD    G + C C  G TG  CE  D  LT++     +G D  +E+       K   IE+ F T +  +GLLLY   +N    +        FI L+    K         + F ++LGSG   + S   +    W  +   R  ++G+L+L+      G +   L  LNL+  L++GG+         ++ + AG   G VG I+ L +N KT   Q++   +      + +      + NPC N G+C+  +  +LC C   + G  C+
Sbjct: 1399 NYRCICP----PQFTGRNCE--ESLDPCIGEPCQHGATCDILPQGGYVCKCPPGRTGEHCEILDAELTELLIPEMSG-DGFLELPCLEGVAKAFSIELWFLT-HASDGLLLYNGQLNNGRGD--------FISLNLVQAK---------LEFRFNLGSGIANITSPDPVTLDTWHCVRISRLGREGVLQLDDGTVARGLSGSPLTELNLEMPLYVGGLK-----HWREIHRLAGARTGLVGAIQRLMVNGKT--YQNLAVNVTQHNTEIYDGLPCPSNENPCHNGGVCLPLLNSYLCKCATGYNGLHCE 1675          

HSP 3 Score: 92.0485 bits (227), Expect = 6.844e-18
Identity = 91/310 (29.35%), Postives = 131/310 (42.26%), Query Frame = 0
Query:  190 SGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQT-CKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            + +N E C  + C +GA+C+V +++G   C C   DC    ++PVCGSD++TY N C L ++ C +R +  V +   CE    C    C  G  CVR+                           G +   Y S C M   AC ++  N+TM+    C  C    C   E C   E S +  C C E       E  PVCG+D  TY + CSLR   C  +    +R  Y G C +       PC ++ C     C  +   + S  C  E +        VC   G+TY +  +L+R AC  +  I VA
Sbjct:   53 ASNNAEPCEKTYCSWGATCVV-SENGKPLCQCP-TDCPS-TSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLNCSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAAC-AKGANITMAFQGKCDPCGNVECVEPEICQLDE-SRQPGCRCGE---QCGLEFAPVCGSDGKTYSNECSLRQEACRSRL--SLRKVYNGACSS----GINPCDEAKCGPYEQCVINRQGIASCECGAECE---PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVA 345          

HSP 4 Score: 87.4261 bits (215), Expect = 2.051e-16
Identity = 80/315 (25.40%), Postives = 140/315 (44.44%), Query Frame = 0
Query:  180 IRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCP-ECPSA---GNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +RK+     SSG N   C  + C     C+++ + G + C+C G +C   + +PVC     TY + C+L +  C  R N+ V     C     C  K C +G  C   G                  G++   Y ++C + L+ C+ R   + +     C  C ++ C++  +C  ++  G++ C CP +C  +     +  + +CG+D +TY + C+++ ++C+ +    + V Y G C          C +  C+ GA C     CVC   C E    P      VCG D +TY +E +L++ AC    ++ V
Sbjct:  268 LRKVYNGACSSGINP--CDEAKCGPYEQCVIN-RQGIASCEC-GAECE-PVMRPVCARGGKTYTSLCELKRQACLTRTNIEVAYTGTCGSRGPCSEKICQWGAICAEIGGTAICECPTCPAEFQPVCGDDGISYGNECQLRLEGCKHR-REIRVLYQGLCNECENKKCEFYAEC-KTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQA--LITVNYVGDCEL--------CARVECEHGAHCMA-GVCVCPDECPESGGEP------VCGSDAKTYPSECELQKAACGRDPKLPV 558          

HSP 5 Score: 80.8777 bits (198), Expect = 2.079e-14
Identity = 90/334 (26.95%), Postives = 129/334 (38.62%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK---------------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDV-------------------RVKYPGVCRTIKYEKPI-------PCGKSSCDFGAVCE--EDSY--CVCSVTC---KEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEI 469
            C    C++GA C      G + C+C    C     +PVCG D I+Y NEC L   GC  R  + V+    C E   C +K C F   C    G   K                            Y ++C M++ +C S+   +T++   +C  C++      +C +       +C CP ECP +G E   PVCG+D  TY S C L+ + C +     V                    +  P V R       +        C   +CDF A CE   D Y  C C   C    +E   P      VCG D RTY +   ++  AC+ Q+E+
Sbjct:  356 CSEKICQWGAICA--EIGGTAICECP--TCPAEF-QPVCGDDGISYGNECQLRLEGCKHRREIRVLYQGLCNE---CENKKCEFYAECKTDNGGEAKCVCPSKCEISTKDAAQAAEKICGSDGVTYANECAMKVASCTSQAL-ITVNYVGDCELCARV-----ECEHGAHCMAGVCVCPDECPESGGE---PVCGSDAKTYPSECELQKAACGRDPKLPVLHVIFYGDCGERFAVAALTTMSTPAVVRLATTAVDVTSTQVREACKNINCDFEATCELGPDDYPRCSCKFDCASISQENMRP------VCGSDLRTYSSLCAMKMEACQRQQEL 666          

HSP 6 Score: 72.0182 bits (175), Expect = 1.077e-11
Identity = 68/257 (26.46%), Postives = 105/257 (40.86%), Query Frame = 0
Query:  239 HITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEE-EDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACK 464
            H++YK +   ++    K +N    +  PCE+ +      C +G +CV +                     G++N  Y + CH+   +C  R +    +       D C   +  C     C+ S    ++ C C E CPS G+ E   PVCGTD   Y S+C +  + C+K  G ++ + + G C         PCG   C    +C+ D      C C   C  E          VCG DG+TY  E  LR+ AC+
Sbjct:   35 HMSYKPQRPTIEG---KDLNFASNNAEPCEKTY------CSWGATCVVSENGKPLCQCPTDCPSTSEPVCGSDNVTYTNYCHLRKSSCLERKSTRVKNQGACEIKDPCTKLN--CSQGSQCVRSRDGSEASCECLESCPSLGDHEGSSPVCGTDGTDYPSLCEMNRAACAK--GANITMAFQGKCD--------PCGNVECVEPEICQLDESRQPGCRCGEQCGLE-------FAPVCGSDGKTYSNECSLRQEACR 263          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1101350313|ref|XP_018901101.1| (PREDICTED: agrin-like isoform X3 [Bemisia tabaci])

HSP 1 Score: 199.904 bits (507), Expect = 1.498e-48
Identity = 141/463 (30.45%), Postives = 214/463 (46.22%), Query Frame = 0
Query:  404 PIPCGKSSCDFGAVCEEDS---YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTR----------------GATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGS-----LCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCIS-QVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVC-HIKDRGFVCVC 839
            P  C +  C F ++CEE +    C+C++TC EE    +     VCG DG+TY +E  L+ +AC+ QK+I V     C                    + +G+ C  +E DC   +S C++  C+C E         +C +  I        CD  PC  GG C +   G F C C  G  G+ C       +   A+F GQ S+ ++     +  ++ IE+  +T+    G+LLY Q       +        FI L+  N           + F Y+LG+G V + S  K+   K+  +VAKRY +DGLL+++  + V G + G+LR L+L    ++G V  N    + KV +N GTS G  GCI+ +KI + T+ L    +P +++   + +C EN CS  PC N   C     + + C C   FTG  C+ R  D C  NPC     C  +   GF C C
Sbjct: 1203 PATCAEMHCYFESICEERNGLPKCLCNMTCPEESNSNQ----VVCGNDGQTYASECQLKLYACRYQKDISVQYLSACKDLFSEEGKDSTPAIVEVMHSSLGEMCSSDE-DCRVSHSHCLSGLCVCLENYVQTQDRQACYLDAIDPTAEVLACDSLPCLNGGRCRNLPHGLFECICEEGYQGTLCAEIAERKEYKIAAFNGQ-SYAQLH-SIKAYHKLSIEMELKTY-ANNGILLYDQQHEDGTGD--------FISLAIVNG---------FVEFRYNLGNGPVVIVSPHKVTLRKFHRIVAKRYQRDGLLQVDSAEPVAGQSLGTLRALDLSQDAYVGFVPTN----VSKVHENIGTSHGLNGCIRHMKIGRHTVYLHGGEDPFIVKVNQIEDCGENPCSSAPCHNGATCKPLDAESYACTCPDSFTGVQCESRM-DPCVSNPCSFGSTCVSLHQNGFTCKC 1635          

HSP 2 Score: 106.686 bits (265), Expect = 1.259e-19
Identity = 92/326 (28.22%), Postives = 148/326 (45.40%), Query Frame = 0
Query:  490 HEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEH-DLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRR 812
            H  + C  L++E  +  C CP+  +       CE       C  NPC  G TC   H   F+C C  G  G+ C+H D  L D  T  F  +++++E+    ++ +   +E+ F      +G++LY GQ  N             FI L+  N          ++ F +DLGSG   + +   I   +W  +   R  + G+L ++ T  V G++   L  LNL+ HL++GGV     P + +V + AG + G  G ++ + +N   I L + R   V + +G       S   NPC N GLC+  +  F C C   F G +CQ++
Sbjct: 1579 HNGATCKPLDAE--SYACTCPDSFT----GVQCESRM--DPCVSNPCSFGSTCVSLHQNGFTCKCLPGRKGNMCQHFDPDLEDFFTPEFAEENAYIELPKLENAGRSFSLEVWFMARE-PDGVILYNGQMTNGKGD---------FISLNLVNG---------YLQFRFDLGSGIANITTPEPISLNEWHKVEMSRMDRRGVLRVDNTTTVEGTSGAPLNELNLELHLYVGGV-----PSLSEVSREAGITAGLNGAVQRVLMNGLPIRLFNRRSNQVAKYQG----PPCSVVDNPCLNGGLCLPLLSSFACKCHPPFIGATCQQQ 1868          

HSP 3 Score: 105.916 bits (263), Expect = 2.281e-19
Identity = 89/282 (31.56%), Postives = 132/282 (46.81%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-HLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYS---CS-QTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIG 485
            PVCGSD  TY N+C+L    C  + NL +I    C    + C S  C FG  CV                       G N + Y + CH++   C+++ T++ ++    C     CS + C++   C+  E+ G  +C CP C +    E DPVCG+D ITY + C LR  +C   R   +R+ + G+C +        C    C+   +C+ DSY    CVC  +C  EE         VCG DG TY +E +LR  +CK ++ + VA    C  GA++ G
Sbjct:  575 PVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFGIAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAK-TDVKLAYAGICGGNGPCSARKCEFGATCV--ERGGSPVCECPVCST----ELDPVCGSDGITYGNECKLRLESCQHHR--QIRILHHGLCNS--------CENKKCETYEICQNDSYGEAHCVCPPSCPPEES-------PVCGSDGATYRSECELRLESCKSRQLVTVASQGDC--GASKTG 830          

HSP 4 Score: 95.5153 bits (236), Expect = 3.603e-16
Identity = 86/309 (27.83%), Postives = 125/309 (40.45%), Query Frame = 0
Query:  195 EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKI--------------------------YKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            E C  + C  GA C  D + GD+ C C   +C       VCGSD  TY++ C L  A C  + ++ V     CE A  C    CPFG  C R+   N+ +                          Y   C +   AC  +L N+T+     C  C+       +    +     +C C E CP     E  PVCG+D  TY + C+LR   C  K+  ++R+ Y G C +       PC    C FG  C  + +    C C   C             VCG +GRTY+T   L++  C+ + ++ +A
Sbjct:  390 EPCEKTTCAHGALCSADER-GDARCHCPA-ECPEGGGPSVCGSDGNTYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFSCPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKL-NITIKYSGKCDPCAGVVCPEPELCQLDADRNPVCKCGEACPL----EFAPVCGSDGKTYGNDCNLRLEACRSKK--NLRIIYRGKCSS----GVNPCASVRCTFGEECVINKFGIAQCECISNCDP-------ILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLA 678          

HSP 5 Score: 80.1073 bits (196), Expect = 1.684e-11
Identity = 119/467 (25.48%), Postives = 179/467 (38.33%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVI--------SDLPCEEAHLCGSKGCPFG-GSCVRTG----------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR------------TIKYEK----PI-----------------PCGKSSCDFGAVCE--EDSY--CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV--------ADFKPCTRGATRIGDYCLHEESDCLA--LNSECVNN----------RC------------------ICPEGTSPNSA--NTSCEIM----KIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCE 563
            C    C FGA+C+   + G   C+C    CS  ++ PVCGSD ITY NEC L    C     + ++         +  CE   +C +    +G   CV             G++   Y+S+C + L++C+SR   +T++S  +C + S+T              K+L  C    SA NE   PVC +D +TY S C +    C+ ++     V Y G C+               +EK    P                   C    CDF A CE   D Y  C C   C  E          VCG D + Y++   ++  AC+ QKE+ +         + KPC  G   + D     E DC +     +C ++          RC                   CP+  +P S   N  C  +    K+GS  D          +  +GT  C+CR G+ G +C+
Sbjct:  689 CSARKCEFGATCV--ERGGSPVCECP--VCSTELD-PVCGSDGITYGNECKLRLESCQHHRQIRILHHGLCNSCENKKCETYEICQNDS--YGEAHCVCPPSCPPEESPVCGSDGATYRSECELRLESCKSRQL-VTVASQGDCGA-SKTGVAASGAEAEGCDEKALAVCGGNGSAANE---PVCASDLVTYPSECEMVRRACTAQKRPLPTVLYYGDCKEKFALGPSSLTTPTTFEKLSLQPTDSSSLENSVFSNSAEKEACKDIHCDFDATCELGPDGYPRCSCLFDCSREA------TKLVCGSDLKVYQSSCAMKMEACQQQKELRLRPLDLCQGMEVKPCN-GEQPLVDPTSGHELDCGSGPRRQDCPSDSYCHQTPYFARCCRKDAKIFLKNCADSWYGCCPDNKTPASGPDNAGCPSLCGCNKLGSYSD----------NCKNGTEQCHCRPGVGGIKCD 1126          

HSP 6 Score: 78.9518 bits (193), Expect = 4.311e-11
Identity = 87/317 (27.44%), Postives = 127/317 (40.06%), Query Frame = 0
Query:  278 CPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSS-----HDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNE-EEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSV----TCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV-------ADFKPCTRGATRIGDYCLHEESDCLALNSECVNN-----RCICPEGTSPNSANTSCEIMKI------------------GSLCDPNPCQGGGTCDEHDGTFSCYC 553
            CP GG     G + N  Y+S CH+ L AC ++ +     S      D C   S  C +   C  S++    +C CPE CPS G+     P+CG+D   Y   C L  + C  K   ++ +KY G C         PC       G VC E   C         CK  E  P  +A  VCG DG+TY  + +LR  AC+ +K + +       +   PC       G+ C+  +        EC++N     R +C  GT+  + +T+C + +                      C    C+ G TC E  G+  C C
Sbjct:  419 CPEGGGPSVCGSDGN-TYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFS--CPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKL--NITIKYSGKCD--------PCA------GVVCPEPELCQLDADRNPVCKCGEACPLEFA-PVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFG--IAQCECISNCDPILRPVC--GTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVCEC 711          

HSP 7 Score: 75.0998 bits (183), Expect = 5.945e-10
Identity = 86/314 (27.39%), Postives = 134/314 (42.68%), Query Frame = 0
Query:  173 SDKLIRVIRKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQK 467
            S K +R+I +         S V  C    C FG  C+++ K G + C+C  I     I +PVCG++  TY   C L Q+ C  + ++ +     C     C ++ C FG +CV  G                  G++   Y ++C + L++C+     + +  H  C SC ++ C+  E C  ++  G++ C    CP +   EE PVCG+D  TY S C LR  +C  ++   V V   G C   K         +S      C+E +  VC        +        VC  D  TY +E ++ R AC  QK
Sbjct:  597 SKKNLRIIYR-----GKCSSGVNPCASVRCTFGEECVIN-KFGIAQCEC--ISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVCECPVCSTELDPVCGSDGITYGNECKLRLESCQHH-RQIRILHHGLCNSCENKKCETYEIC-QNDSYGEAHCV---CPPSCPPEESPVCGSDGATYRSECELRLESCKSRQ--LVTVASQGDCGASKTGV-----AASGAEAEGCDEKALAVCGGNGSAANE-------PVCASDLVTYPSECEMVRRACTAQK 883          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207770|ref|XP_019637523.1| (PREDICTED: agrin-like isoform X5 [Branchiostoma belcheri])

HSP 1 Score: 199.519 bits (506), Expect = 1.790e-48
Identity = 233/859 (27.12%), Postives = 341/859 (39.70%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC-------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSANT----------------SCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC             E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA+                 + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1618          

HSP 2 Score: 105.145 bits (261), Expect = 3.380e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.5969 bits (244), Expect = 3.339e-17
Identity = 110/421 (26.13%), Postives = 168/421 (39.90%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC     R+       + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPC---PDRVPP---APQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 965          

HSP 4 Score: 87.8113 bits (216), Expect = 8.454e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1453 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1756          

HSP 5 Score: 82.4185 bits (202), Expect = 3.233e-12
Identity = 71/262 (27.10%), Postives = 102/262 (38.93%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K            +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1710 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQ---------LTYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1939          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1080047455|ref|XP_018565030.1| (PREDICTED: agrin-like [Anoplophora glabripennis])

HSP 1 Score: 196.438 bits (498), Expect = 1.655e-47
Identity = 169/603 (28.03%), Postives = 270/603 (44.78%), Query Frame = 0
Query:  327 TCKWNEDCLYSEKSGKSLCSC-PECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEE-EQFPKYYAGAVCGXDGRTYETE--XDLRRHACKMQKEIVV----ADFKPCTRGA--------TRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSC----EIMKIGSLCDPNPCQGGGTC-DEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD-RGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL 907
            TC +   C+  E+ G ++C C  +CP  G  E   VCG+D  TY+S C LR   C  ++  D+ V+  G C+   +       +          +DS    S + +      P+YY          +Y      D   +   +   ++      DF P  R            +GD C  + +DC+ L S CV   C C    S +S    C    +       C  +PC    TC D    TF+C C S  TG  CE ++        SF G+ S+  +K P  +  ++ IE+ F++ +  EG+LLY Q          +     F+ L+  N           + F Y+LG+GAV ++S  KI+ GK+  +V KRYH+DG+L+L+  + V G   GSL+ L+L    ++G V  N      +V +N GT+ GF GCIK+LKI ++ I L   +   V++ +G+ +C E++C+ +PC N G C++    + C C+  ++G  C++   D C   PCQ    C   D   F+C C      G +C+L+          Y  A+     FN T      +  G+ ++    +E+SF+ K    L
Sbjct:  574 TCYFGAACI--ERGGFAMCECHAKCPEDG--EVQVVCGSDGQTYNSPCELRQLACRLQK--DIVVQAFGTCKDDMFPSTDWPVRRYTPLQFTQPDDSNSPLSKSTRHLLVPDPRYYYDRRENIHPVSYPASDNKDSDFNESGLGSNVIYHTRGGDFAPAYRPTPATVRVVTALLGDLC-SDNTDCMILYSHCVKGACTCLPDYSESSDRQECIADSDPTDEYRACSSSPCHHASTCVDLPAATFTCVCSSNFTGPLCETEIFRKQYEVPSFHGR-SYARLK-PLKAYHKLSIEVEFKSHS-DEGILLYNQ--------QKVDGLGDFVSLAIVNG---------FVEFKYNLGNGAVLIRSLDKIQLGKFHRVVLKRYHRDGILKLDDAEDVAGQAMGSLKALDLLEDTFVGYVPTN----HTRVYENIGTARGFQGCIKKLKIGRQEIELHMNQHDWVLKTKGIHDCGEHACTHSPCLNNGKCLANDDLYRCECQPRYSGDFCEKPE-DPCLSVPCQYGSKCKALDFDSFICECPPGR-SGQHCELE----------YKDARMATPEFNGTSFIRFPRLEGIKKK--FSIEISFMPKASNGL 1131          

HSP 2 Score: 92.0485 bits (227), Expect = 3.645e-15
Identity = 72/245 (29.39%), Postives = 104/245 (42.45%), Query Frame = 0
Query:  232 KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSY----CVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            +PVC  D  T+ ++C++ +AGC  R    +          +CG KG                                      S H+        C++   C+  E+SG + C CP CP+    E +PVCG+D I+Y + C LR   C  +R  D+ V Y G C          C    CDF ++CE D      CVC  +C +    PK   G VCG DG TY  E +LR  +CK ++ I++A
Sbjct:    2 RPVCSKDGRTFPSDCEMKRAGCLARTTTEIAY------TGVCGEKG------------------------------------PCSEHE--------CQYGATCV--ERSGTAHCECPICPA----EFEPVCGSDGISYGNECKLRLEACKHRR--DITVLYNGPCNG--------CENKKCDFYSICESDGATEGRCVCPQSCTD----PKLTNGTVCGTDGVTYANECELRAGSCKTKQYILLA 176          

HSP 3 Score: 89.7373 bits (221), Expect = 1.815e-14
Identity = 92/349 (26.36%), Postives = 141/349 (40.40%), Query Frame = 0
Query:  478 TRGATRIGDY-CLHEESDCLALNSECVNN----RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHD-GTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRR----------GLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQ 810
            T+G    G++ C H  S CL  N +C+ N    RC C     P  +   CE  K    C   PCQ G  C   D  +F C C  G +G  CE +     M T  F G  S +         K+  IEI F       GL+LY   +     +        FI L+             H+ F ++LGSG   L S   +   KW      R  + G+L+L+ +  V G +   L  LNL+   ++G +         ++ + +G+S GF G I+ + +N   + + ++                   G  +    S + NPC N GLC+ +++ F C C   + GK C+
Sbjct: 1004 TKGIHDCGEHACTH--SPCLN-NGKCLANDDLYRCEC----QPRYSGDFCE--KPEDPCLSVPCQYGSKCKALDFDSFICECPPGRSGQHCELEYKDARMATPEFNGT-SFIRFPRLEGIKKKFSIEISFMP-KASNGLILYNGQMKNGRGD--------FISLNLVGG---------HLQFRFNLGSGIANLTSREAVTLDKWHWARISREGRGGVLQLDNSSIVRGFSGIPLTELNLETPFFLGSLSN-----WKEIHRLSGSSEGFRGAIQRIIVNGNPLPISAVLPYCSTSTSHNKSGCSFNIGYYDGLPCSANKNPCLNNGLCVPELEDFTCQCPSSYKGKYCE 1319          

HSP 4 Score: 82.4185 bits (202), Expect = 3.444e-12
Identity = 89/371 (23.99%), Postives = 144/371 (38.81%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG-----------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCP-ECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGN---------DVRVKYPGVCRTIKYEKPI-------------------------------PCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV--------ADFKPCTRGATRIGDYCLHEESDC 495
            C    C++GA+C+   +SG ++C+C    C     +PVCGSD I+Y NEC L    C  R ++TV+ + PC   + C +K C F   C   G                       G +   Y ++C +   +C+++   + ++   NC  C        +C Y  +     C CP  C  +G+E   PVC ++ +TY + C L+ + C + R +         D R ++P          PI                                C    CDF A CE         TC+ +       A  VC  + +TY +   ++  AC+ Q+E+ +         + KPC +G   + D     E DC
Sbjct:   43 CSEHECQYGATCV--ERSGTAHCECP--ICPAEF-EPVCGSDGISYGNECKLRLEACKHRRDITVLYNGPC---NGCENKKCDFYSICESDGATEGRCVCPQSCTDPKLTNGTVCGTDGVTYANECELRAGSCKTK-QYILLAYKGNCDLCQGV-----ECKYGARCEAGECVCPTNCEGSGDE---PVCASNMMTYPNECELQKAMCLQPRSSPPLSVVFYGDCRERFPAAGSFSTTLTPITAQTNNMATGSDVEPASPDNIIPDGGNTEKEACRDIHCDFEATCELGPDHFPRCTCQFDCASAALSAKPVCASNLQTYSSLCAMKMDACQRQEELRLRPLDLCQGMEVKPC-KGEKPLTDPATGRELDC 395          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207762|ref|XP_019637519.1| (PREDICTED: agrin-like isoform X1 [Branchiostoma belcheri])

HSP 1 Score: 196.438 bits (498), Expect = 1.915e-47
Identity = 233/870 (26.78%), Postives = 341/870 (39.20%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSANT----------------SCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA+                 + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1629          

HSP 2 Score: 105.531 bits (262), Expect = 2.558e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.9821 bits (245), Expect = 2.615e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 87.8113 bits (216), Expect = 7.025e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1464 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1767          

HSP 5 Score: 82.8037 bits (203), Expect = 2.779e-12
Identity = 71/262 (27.10%), Postives = 103/262 (39.31%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1721 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1950          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207776|ref|XP_019637526.1| (PREDICTED: agrin-like isoform X8 [Branchiostoma belcheri])

HSP 1 Score: 196.438 bits (498), Expect = 2.132e-47
Identity = 226/837 (27.00%), Postives = 332/837 (39.67%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T              A+      N     +  S      + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTA------------AVTPTASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1584          

HSP 2 Score: 105.531 bits (262), Expect = 3.062e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.9821 bits (245), Expect = 3.026e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 87.8113 bits (216), Expect = 7.604e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1419 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1722          

HSP 5 Score: 82.4185 bits (202), Expect = 3.275e-12
Identity = 71/262 (27.10%), Postives = 103/262 (39.31%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1676 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1905          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207766|ref|XP_019637521.1| (PREDICTED: agrin-like isoform X3 [Branchiostoma belcheri])

HSP 1 Score: 196.052 bits (497), Expect = 2.336e-47
Identity = 233/870 (26.78%), Postives = 340/870 (39.08%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSA--NTSCEIMKIGSL------------------------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA  N      K+ +                         C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1629          

HSP 2 Score: 105.531 bits (262), Expect = 2.927e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.9821 bits (245), Expect = 3.312e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 87.8113 bits (216), Expect = 7.018e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1464 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1767          

HSP 5 Score: 83.9593 bits (206), Expect = 1.221e-12
Identity = 73/254 (28.74%), Postives = 104/254 (40.94%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-TDMNTASFTG--QDSHVEIKIPTDSVKRM------EIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S  +D       G   D    +  P    K++        E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1721 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1942          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207774|ref|XP_019637525.1| (PREDICTED: agrin-like isoform X7 [Branchiostoma belcheri])

HSP 1 Score: 196.052 bits (497), Expect = 2.703e-47
Identity = 233/870 (26.78%), Postives = 341/870 (39.20%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSANT----------------SCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA+                 + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1629          

HSP 2 Score: 105.531 bits (262), Expect = 3.128e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.9821 bits (245), Expect = 3.252e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 92.8189 bits (229), Expect = 2.462e-15
Identity = 84/332 (25.30%), Postives = 145/332 (43.67%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+      G + G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1464 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLDGKVVGTGESPGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1758          

HSP 5 Score: 83.9593 bits (206), Expect = 1.219e-12
Identity = 73/254 (28.74%), Postives = 104/254 (40.94%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-TDMNTASFTG--QDSHVEIKIPTDSVKRM------EIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S  +D       G   D    +  P    K++        E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1712 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1933          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207768|ref|XP_019637522.1| (PREDICTED: agrin-like isoform X4 [Branchiostoma belcheri])

HSP 1 Score: 195.667 bits (496), Expect = 3.201e-47
Identity = 233/870 (26.78%), Postives = 341/870 (39.20%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSANT----------------SCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA+                 + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1629          

HSP 2 Score: 105.531 bits (262), Expect = 3.186e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.5969 bits (244), Expect = 3.426e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 92.4337 bits (228), Expect = 2.945e-15
Identity = 84/332 (25.30%), Postives = 145/332 (43.67%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+      G + G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1464 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLDGKVVGTGESPGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1758          

HSP 5 Score: 82.8037 bits (203), Expect = 2.848e-12
Identity = 71/262 (27.10%), Postives = 103/262 (39.31%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1712 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1941          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207772|ref|XP_019637524.1| (PREDICTED: agrin-like isoform X6 [Branchiostoma belcheri])

HSP 1 Score: 195.667 bits (496), Expect = 3.547e-47
Identity = 230/855 (26.90%), Postives = 339/855 (39.65%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGT--SPNSANT----------------SCEIMKIGSL----------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          +D  ++  E      +  E T   P SA+                 + E M+              C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  828 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTENATDKPSM-PEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1613          

HSP 2 Score: 105.531 bits (262), Expect = 2.685e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARY-DPVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 98.9821 bits (245), Expect = 2.676e-17
Identity = 110/426 (25.82%), Postives = 167/426 (39.20%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKY------------------EKPIPCGK-SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++                   E+   C + S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 976          

HSP 4 Score: 87.8113 bits (216), Expect = 6.894e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1448 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1751          

HSP 5 Score: 82.8037 bits (203), Expect = 2.919e-12
Identity = 71/262 (27.10%), Postives = 103/262 (39.31%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1705 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1934          
BLAST of EMLSAG00000004167 vs. nr
Match: gi|1126207764|ref|XP_019637520.1| (PREDICTED: agrin-like isoform X2 [Branchiostoma belcheri])

HSP 1 Score: 195.667 bits (496), Expect = 3.660e-47
Identity = 233/870 (26.78%), Postives = 340/870 (39.08%), Query Frame = 0
Query:  200 SNCRFGASCI-VDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC------------------------EEAHLC-----------GSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPI---PCGKSSCDFGAVCEEDS----YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDC-----LALNSECVNNRCICPEGTS------------PNSA--NTSCEIMKIGSL------------------------CDPNPCQGGGTCDE-HDG-TFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKI-----NKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLC-ISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIK-DRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSL----------GRLKISSTKTGLLKLQLSDSQGALTEWITSPPFFVSDNRPHNIIL--NIYRGELDLD 962
            SNCRFG  C+ +D  S    C C+ I CSG I KPVCG+D  TY+NEC L  A CT + ++ V+S+ PC                        E  + C           G  GCP    C   G      Y   C  +   C  +   +     D C    +   WN       + GK  C+  EC   G+  +D     D +T   VC    S     R    +V  P  CR     +P     C +  C +GAVC++ S     CVC   CK      K     VCG DG+T+  E  +R  AC+ Q++I VA   PC    T          + C         SE   ++   PEGT             P SA  N      K+ +                         C   PC  GGTC   +DG +F C C +G TG  CE ++        +F G DS++  K          I + FR +  ++GLL+Y          N  ++   FI L+  N K         + F YDLGSG   +     +E  +W  + A R  +DG + +       G++ G+   LNL    ++G + P D   +Y   +  G + GF GCI+ELKI      +K   L+   + ++    G+ EC  + C   PC+NEG C ++    F C CK+ F G  C    +D CDP+PC  + +C +K + GF C C      G  CD +  D     P +              ++  +  L    ++L    + +  K+ G L          G     + K G L  + +  +G        P   V+ N  H + L  N+ RG++ LD
Sbjct:  827 SNCRFGGKCMDMDPDSEGEECICE-IKCSGDI-KPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKCCPDGKTPAAGAGFAGCPSVCKCNSLGS-----YAQTCDSQSGQCSCK-PGVGGKKCDRC----EPGFWN---FRGMQDGKIGCTPCECSKGGSVRDD----CDQMTGKCVCKPGVSGMKCDRCRKGQVMGPTGCREPTQREPEVAESCDQLMCKYGAVCKKMSRDVAQCVCPTECKRGTIETK-----VCGSDGQTFADECQMRVIACRYQQDIRVAYAGPCKADVTTAAVTPTGRNTTCPTNLTRTPASENATDKPSMPEGTKETVLPTESTRVPPTSASVNLVTPTKKMSTFPPLKTTEKMRPPVTVTPPPPAPTPCSSVPCMHGGTCKRTNDGRSFECSCPAGWTGPVCEEEVFFYQ---PAFGG-DSYIAFKT-IKVFLSGTIMLDFR-YTGKDGLLVY----------NGQKSGKDFISLAIVNGK---------LQFQYDLGSGPSEIIHSLPLEPNRWYAVTASRVKRDGYIRIGNEPDTTGASPGATTGLNLDDDFYVGNIPPEDRDRVY---QRIGVTGGFKGCIRELKIIDQKGQEKMYDLRPNGKDMMF-GVGVSECGSDPCKAKPCQNEGQCEVTNDGNFRCVCKQGFHGPLCGHAASDPCDPDPCHPDSLCTMKPEGGFQCRCPVGR-RGMLCDEEIEDAQTFIPEFSGN-----------SYIQRAGLTAVSKSLSAEVIFYATKNNGMLLYNGQKTDGKGDFVSLNLKDGYLVFRFNLGKGPADIRSEDP---VTLNEWHEVKLDRNLRRGQMTLD 1628          

HSP 2 Score: 105.531 bits (262), Expect = 3.083e-19
Identity = 100/370 (27.03%), Postives = 158/370 (42.70%), Query Frame = 0
Query:  144 AVSLSEGKRVFFLEHVDESLRLKFPSLL----------ASDKLIRVIRKLLETEASSGSNV----EGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAH--LCGSKGCPFGGSCVRTGGN-------------------NNKIYKSQCHMELDACESRLTNLTMSSHDNCYSC-SQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
              SL  G+ V  +   D S+++ F  +L            +KL+ V++   + E   G+ V    E C    C F + C ++ ++ D+ C C+ I C      PVCG + +TY N+C   QA C ++  + V  +  C  A   LC +  C FG +C    GN                   +   Y + C+++ ++CE R   +       C  C + TC++   C  S  S ++LC CP+   +  E   PVCG+D  TY + C L  ++C++++   +R      C  +       CG + C     C +D  C C   C      P  Y   VCG DG TY +E  L   AC+    +VV    PC
Sbjct:  447 GASLGPGREVKIIGFKDGSIKVIFQIVLPPGSRENPEDVKEKLMEVLQDAPDLEIKPGTLVVEEAESCFGQVCEFSSECTINPRTRDADCSCESITCD-ESYVPVCGDNMVTYNNDCKRKQAECQEQKPIIVKYNGKCAPAKEALCENVKCGFGATCTVIEGNAMCECDTVCPAEFQPVCGSDGVSYNNMCYLQAESCE-RQQEIRKVDDRLCNGCENTTCRFGAIC-QSSSSVEALCVCPK---SCIEVYSPVCGSDGKTYSNKCELEVASCNQQK--HIRAVADMPCEDMD------CGGAHCGPYQECVDDK-CQCPTVC------PARYD-PVCGSDGITYNSECKLAVKACQDGLSVVVKAPGPC 794          

HSP 3 Score: 102.834 bits (255), Expect = 2.066e-18
Identity = 111/425 (26.12%), Postives = 167/425 (39.29%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG--------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR-TIKYE-------KPIPCGK-----------SSCDFGAVC------EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR-----IGDYCLHEESDCLALNSECVNNRCICPEGTSPNSAN------TSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEH 564
            C +  C FGA+C V    G++ C+C  + C     +PVCGSD ++Y N C L    C ++  +  + D  C   + C +  C FG  C  +                     G++ K Y ++C +E+ +C  +     ++       C         C   ++     C CP  CP+      DPVCG+D ITY+S C L    C  + G  V VK PG C  T++ E       +P   G            S+C FG  C       E   C+C + C  + +        VCG D +TYE E  L+  AC +Q+ I V    PC             D     + DC     +C      CP+G +P +        + C+   +GS           TCD   G   C C+ G+ G +C+ 
Sbjct:  591 CENVKCGFGATCTV--IEGNAMCECDTV-CPAEF-QPVCGSDGVSYNNMCYLQAESCERQQEIRKVDDRLC---NGCENTTCRFGAICQSSSSVEALCVCPKSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVAD----MPCEDMDCGGAHCGPYQECVDDKCQCPTVCPA----RYDPVCGSDGITYNSECKLAVKAC--QDGLSVVVKAPGPCEDTVEVEGSGSGSGEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIK-------PVCGTDKKTYENECRLKLAACTLQRHIQVMSNGPCPDRVKVFRQQFFSDVPPAPQKDCKETTYKC------CPDGKTPAAGAGFAGCPSVCKCNSLGSYAQ--------TCDSQSG--QCSCKPGVGGKKCDR 975          

HSP 4 Score: 87.4261 bits (215), Expect = 8.982e-14
Identity = 86/343 (25.07%), Postives = 148/343 (43.15%), Query Frame = 0
Query:  504 NNRCICPEG-TSPNSANTSCEIMKIGSLCDPNPCQGGGTCD-EHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLY-GQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGS-----------TRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC---EENSCS--GNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGV 827
            N RC+C +G   P   + + +       CDP+PC     C  + +G F C C  G  G  C+ ++         F+G +S+++    T   K +  E++F       G+LLY GQ  +             F+ L+  +          +++F ++LG G   ++S   +   +W  +   R  + G + L+   KV+G+             G+L  LNL   L+IGG +  D+    +   +A  + GF G I++L I    +        LV      V+    E++SC+   NPCKN G C+  +  + C C   +TGK C++  +   DP P +  G+
Sbjct: 1463 NFRCVCKQGFHGPLCGHAASD------PCDPDPCHPDSLCTMKPEGGFQCRCPVGRRGMLCDEEIEDAQTFIPEFSG-NSYIQRAGLTAVSKSLSAEVIFYATK-NNGMLLYNGQKTDGKGD---------FVSLNLKDG---------YLVFRFNLGKGPADIRSEDPVTLNEWHEVKLDRNLRRGQMTLD--GKVVGTGESPGGPSNNVLEGTLSQLNLGLPLFIGGGE--DYSTFAR---DADITTGFTGAIQKLVIAGAVVP------DLVDGALAAVDIGNFEKHSCTRKNNPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGI 1766          

HSP 5 Score: 82.4185 bits (202), Expect = 3.129e-12
Identity = 71/262 (27.10%), Postives = 103/262 (39.31%), Query Frame = 0
Query:  534 NPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSL-----------------TDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVI--GSTRGSLRTLNLQGHLWIGGVD---PNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVEC 773
            NPC+ GG C      + C C  G TG RCE   S                  T +N  +   +     I+    +      E+ FRT +   GLLL+       +          FI L+    K  +         +Y LG    T++S   +  G+W  +VA R  ++G LE+   +  I  G +      LN  G LWIGG     P+  P  Y        + GFVGCIK++KI+++ + L        I RR L  C
Sbjct: 1720 NPCKNGGACMPMMADYKCDCSGGYTGKRCEKAPSFGSDPAPLRLEGISLDGTTSLNYPNAINKKESNSIREQVPNQDNNHFEVTFRTTS-DHGLLLWNHKPGGGD----------FIALAIVGGKPQL---------TYKLGKQPFTIESPISVNTGRWITIVADRTKREGSLEIVGVNGSIQKGESPPGASQLNTDGVLWIGGFSGALPSGLPSEY--------NSGFVGCIKDVKIDRQELDLLQNS----IERRNLKPC 1949          

HSP 6 Score: 66.2402 bits (160), Expect = 3.001e-7
Identity = 67/250 (26.80%), Postives = 101/250 (40.40%), Query Frame = 0
Query:  196 GCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG---------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEED-------------------------------------PVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYP 393
            GC ++ CRFGA C   + S ++ C C        +  PVCGSD  TY N+C+L  A C ++ ++  ++D+PCE+   CG   C     CV                  G++   Y S+C + + AC+  L+ + + +   C          ED +  E SG S    PE    G+EE D                                     PVCGTD  TY++ C L+ + C+ +R   V    P
Sbjct:  659 GCENTTCRFGAIC-QSSSSVEALCVCP--KSCIEVYSPVCGSDGKTYSNKCELEVASCNQQKHIRAVADMPCEDMD-CGGAHCGPYQECVDDKCQCPTVCPARYDPVCGSDGITYNSECKLAVKACQDGLS-VVVKAPGPC----------EDTVEVEGSG-SGSGEPEGSGTGDEEFDFCDEGSNCRFGGKCMDMDPDSEGEECICEIKCSGDIKPVCGTDKKTYENECRLKLAACTLQRHIQVMSNGP 892          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold421_size176100-snap-gene-0.22 (protein:Tk10554 transcript:maker-scaffold421_size176100-snap-gene-0.22-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_308094")

HSP 1 Score: 393.275 bits (1009), Expect = 1.024e-120
Identity = 282/884 (31.90%), Postives = 423/884 (47.85%), Query Frame = 0
Query:  181 RKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTG---------------GNNNKIYKSQCHMELDAC----ESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCS-CPECPSAGNEEEDPVCGTDNITYDSV-CSLRASTCSKKRG---------NDVRVKYPGVCRTI---KYEKPIPCGKSSCDFGAVCEEDS---YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT--------------------------------------------RIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMK--------IGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTF--------PFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI--KDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLS---DSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDV 963
            ++ +E         E C+   C     CI D  +G+  C C+G  CS + ++ VCGSD ITY + C L +A CT+  ++       C+    C    C FG  C                    +N K Y + CH+E D C    ++      +     C      C   + C      G  LC  C     A        CG +    +S  C  +   C  KRG          D  +     C ++   KYE+PIPCG+S C+FGA C        C+C + C+     P YY  AVCG DG TY +E  LR+++C+ Q +I + +F PC    T                                            + G+ CL  + DC+  NS C  N+CIC E    ++ + +C+ +         +  LCDPNPC GGGTC+EHDG FSCYC  G  G+ C+HDLS + ++ ASF G +S + +  P D+V R+EI++ FRTF  Q GL+LY +  N+   E    +         P     +   + C+     + + F YDLGSG V L+S+ ++  G+W  LVAKRYH+DGLLE++  D+V GS++GSLRTLN+   +WIG VD         V K  GT IGF GC+K+L++++  I+LQS +EP+V++R+ +++CEEN C   PC NEG C  + QGF C C  +FTG+ CQR+RN  C PNPC+N G C I   +  F C C    ++G  C+    ++  ++    ++K  +   +  L  +L K+  L            ++  PG++G L +  +++G L ++L    +S+  +    T+ P  +SD   H +  NI R +  L V
Sbjct:  126 QQKVEITVKKYGKCETCQDKKCGPHEECIEDT-NGNPKCLCQG--CSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDP---CNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPV-DGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRN----PLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQFGETCL-TDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVG-NSLMAVLAPADAVNRLEIKLKFRTF-AQNGLILYNEG-NLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQ---YEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRN-HCHPNPCKNKGQCQIARNENSFTCSCPVG-FFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIP--------VRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLP--ISDGNWHRV--NILRQDSSLIV 976          

HSP 2 Score: 131.339 bits (329), Expect = 3.469e-31
Identity = 110/372 (29.57%), Postives = 167/372 (44.89%), Query Frame = 0
Query:  221 CKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVR----------------------TGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCS-QTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANT-SCEIMKIGSL-CDPNPCQGGGT----CDEHDGTFSCYCRSGLTGSRCE 563
            C  ID   R N PVCGSDH+TY+N C+L +A C K+ N+T ISD  CE+A+LC S  CPF G C R                        G++N  Y ++C +   +C+ ++  +T+  +  C +C  + C  +E+C+  + +G   C C  C +  +EE   VCG+D ITY S+C L+ ++C++    D+  ++ G C         PC + +C FG+ C E   C+C   C      P  Y   +C  +G+TY     L +  C  Q +     F+             + E   CL     C  + C CP G   +  +          S  C    C   G+    CD   G   C C+ G +GS+CE
Sbjct:   19 CDVID-EARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKV-EITVKKYGKCETCQDKKCGPHEECI-EDTNGNPKCLCQGCSNKPSEE---VCGSDGITYFSLCHLQRASCTQNE--DIFAEHYGACD--------PCNEVTCKFGSYCSE-GQCICRQEC------PDSYE-PICASNGKTYFNYCHLEKDNCARQHDANFEQFR-------------VVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGV--CPCKRGFSGSKCE 351          

HSP 3 Score: 58.9214 bits (141), Expect = 6.126e-9
Identity = 53/208 (25.48%), Postives = 89/208 (42.79%), Query Frame = 0
Query:  197 CRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVRTGGNNNK--------------------IYKSQCHMELDACESRLTNLTMSSHDNCYSCSQ-TCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKK 383
            C    C F   C  + + G   C C+      R  +PVCGSD++ Y NEC+L ++ C +++ +TV     CE    C  K C     C+     N K                     Y S CH++  +C ++  ++    +  C  C++ TCK+   C      G+ +C   ECP +     +P+C ++  TY + C L    C+++
Sbjct:   70 CSSDPCPFNGVCARNYE-GRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE---TCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASC-TQNEDIFAEHYGACDPCNEVTCKFGSYC----SEGQCICR-QECPDS----YEPICASNGKTYFNYCHLEKDNCARQ 263          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold48_size466083-snap-gene-1.19 (protein:Tk05968 transcript:maker-scaffold48_size466083-snap-gene-1.19-mRNA-1 annotation:"basement membrane-specific heparan sulfate proteoglycan core protein")

HSP 1 Score: 125.946 bits (315), Expect = 1.015e-29
Identity = 109/427 (25.53%), Postives = 179/427 (41.92%), Query Frame = 0
Query:  506 RCICPEGTSPNSANTSCEIMKIGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECE--ENSCSGNPCKNEGLCI--SQVQGFLCHCKRDFTGKSCQRRRNDQCDPN-PCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKK-DPGSLGRLKISSTKT---GLLKLQ 923
            RCIC     P  +   C+  + G +C    C GG  C      F C+C  G  GS CE     T +   +F G +S++ I  P   ++++++   FR  + ++ ++LY                          +   V     H+ F ++ GSG   L+S   I   KW  +   R  + G + +   + V G + GS R LN++  ++ GGV+   + L   V    GT +GF+GCI +++  +KT+ L      +++    + +C   E+ C   PC+N G C          C C  DF G +C+RR   QCD + PCQN G C            G+E+Y  +C             +G     K      L  +   S+G   ++ MEL+   L   DP  +  +++  + T   GL+  Q
Sbjct:  243 RCIC----LPGFSGEHCK--REGEMCFTGACNGG-RCQNTAKDFECFCPFGQEGSFCE---DTTTIERPAFQG-NSYLAIPRPKHILRQLKMAFRFRAVDTRDSVVLYSAQSE-----------------DGQGDFASVTIQDGHLEFRFNTGSGTAILRSPEPIYAKKWYEVALNRKLKAGTMFISGQEPVKGESPGSTRGLNIRTPMYFGGVNKEKYTLAPGV----GTDLGFIGCITDIRFGRKTLDLSK----VIVDSANIRDCSSTESKCDEAPCQNGGTCQPGKSKDQHTCQCPEDFRGDNCERRAT-QCDISMPCQNGGRCE----------GGTEFYKCFCPW----------GFGGRNCEK-----VLPMNASTSVGFRGDSFMELDWKLLPHFDPTKIETVELEFSTTEPQGLIWYQ 607          

HSP 2 Score: 73.9442 bits (180), Expect = 1.320e-13
Identity = 74/264 (28.03%), Postives = 116/264 (43.94%), Query Frame = 0
Query:  507 CICPEGTSPNSANTSCEIMKIGSLCDPN-PCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIK---IPT-DSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWI-GGVDPNDFPLMYKVRKNAGTSI-GFVGCIKELKINKKTISLQSMREPL 763
            C CPE    +    +CE  +  + CD + PCQ GG C+     + C+C  G  G  CE  L +    +  F G DS +E+    +P  D  K   +E+ F T   Q GL+ Y   V     +        F+ LS S+             F ++LG G+  L +   I  GK   L   R  + G ++++  D   GS++G    L+  G+++I GG+ P+        R   G  + GF GC+  L+I  + I  Q  R+P+
Sbjct:  503 CQCPE----DFRGDNCE--RRATQCDISMPCQNGGRCEGGTEFYKCFCPWGFGGRNCEKVLPMNASTSVGFRG-DSFMELDWKLLPHFDPTKIETVELEFSTTEPQ-GLIWYQDGVQKQLKDRN------FMALSISSGTPS---------FDFNLGEGSAELTASKVINDGKRLRLKLIRKARKGSIQIDGRDLAKGSSKGFTGRLSTDGNIFIGGGIKPS--------RLTHGRYVYGFRGCLHRLRIQGREIDFQ--RDPV 733          

HSP 3 Score: 73.9442 bits (180), Expect = 1.663e-13
Identity = 82/329 (24.92%), Postives = 138/329 (41.95%), Query Frame = 0
Query:  553 CRSGLTGSRCEHDLSLTDMNTASFTGQD--SHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELK---INKKTISLQSM-REPLVIRRRGLVECEENSCSGNPCKNEGLCISQV--QGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCS-GSEYYGTYC-DLKTADTPNDQP-AYGSAKAPK 870
            C++  + +  E  ++L   +      Q   S++E+    DS   +++E+ F+   +  GL+LY         +        FI     N K  +          Y+LGS    L +   I  G+W  +   R  ++  L ++    + GS  G    L+L   L++GG      P   +  ++   + GF G    L    I +  +  Q M   P  ++ +G+  C+  SCS +PC+N G+C      QG+ C C   F+G+ C +R  + C    C N G C    + F C C  G E  G++C D  T + P  Q  +Y +   PK
Sbjct:   16 CKANNSDAEVEQSMALIVTHVIPHFNQAPLSYMELVTLPDSYLNLDLELSFKP-ELANGLILYNAHDERGGKD--------FISFGLKNGKPEL---------RYNLGSETAILTAEKPITFGQWHTVKIARSRKNASLFVDDDLPIRGSAPGRFEGLDLMTPLYLGGT-----PSQVQALEDTSLNNGFKGKYLVLVFCCIGRFVVGTQEMVLSPATLKSQGVDTCD--SCSLSPCENGGICQGSANDQGYRCICLPGFSGEHC-KREGEMCFTGAC-NGGRCQNTAKDFECFCPFGQE--GSFCEDTTTIERPAFQGNSYLAIPRPK 315          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold155_size301336-snap-gene-1.5 (protein:Tk03781 transcript:maker-scaffold155_size301336-snap-gene-1.5-mRNA-1 annotation:"serine protease inhibitor dipetalogastin precursor")

HSP 1 Score: 83.1889 bits (204), Expect = 2.943e-16
Identity = 77/270 (28.52%), Postives = 109/270 (40.37%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEE--------------AHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYC 488
            P+CGSD ITY NEC L    C +   +TV    PC                AH+CGS G               K Y ++C+++ +AC++ L +L + S   C +             +E  G      P+CP+      +PVCG+D  TY + C LRA  C  K   D+ VK PG+C  +               G V      C+        E  P  +A  VCG +G TY  E  L+  AC+ + E+ VA    C+         C
Sbjct: 1406 PICGSDGITYSNECTLQAKSCLQSERITVTQSGPCPSDTADDCPAICSTIIAHVCGSDG---------------KTYANECNLKAEACQTDL-DLVLRSQGPCEN-------------TESDGPK----PDCPTTCGAIFNPVCGSDGKTYSNECKLRAKAC--KELLDLTVKSPGLCEDVVINDD--------QEGLV----GDCL--------ENCPTIHA-PVCGTNGNTYPNECSLKTQACQNEPELRVASHGMCSNDEQACAQNC 1619          

HSP 2 Score: 73.1738 bits (178), Expect = 4.015e-13
Identity = 80/290 (27.59%), Postives = 121/290 (41.72%), Query Frame = 0
Query:  228 GRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVR--------TGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNC----YSCSQTCKWN-------------EDCLYSEKSGK-----SLCSCPECPSAGNEEED----------PVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            G I  PVCGSD  TY NEC L    C + ++LTV S   CE+  +   +     G C+           G N   Y ++C ++  AC++    L ++SH  C     +C+Q C  N              +C    K+ +     ++    +C S G+ +++          PVCG+D  TY + CSLRA  C  K  +D+ V+  G C T +  + +P     C    +C                  P  YA  VCG DG+TY  +  L+  AC  Q ++ V     C
Sbjct: 1509 GAIFNPVCGSDGKTYSNECKLRAKACKELLDLTVKSPGLCEDVVINDDQE-GLVGDCLENCPTIHAPVCGTNGNTYPNECSLKTQACQNE-PELRVASHGMCSNDEQACAQNCPANFNPVCGSDGKTYSNECNLKAKACRDQLKLTMVGTGKCQSEGSIDDECPKMCALIFAPVCGSDGNTYGNECSLRAKAC--KDQSDLTVRSVGSCLTEESTQ-VPTESQPC--SKIC------------------PAIYA-PVCGSDGKTYSNKCTLKAKACTEQLDLSVKSMGNC 1772          

HSP 3 Score: 72.7886 bits (177), Expect = 4.154e-13
Identity = 74/268 (27.61%), Postives = 111/268 (41.42%), Query Frame = 0
Query:  224 IDCSGRIN---KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE-------EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGA 481
            +DC  R     +PVCGSD  TY NEC L++  C  +++++++ +  CE       E     ++ CPF    V   G +   Y + C++E  AC   +  L +S    C       +     L  E S        +C      E  PVCG+D  TYD+ C L    C  +   ++ + +                ++SCD   V   +S   C   C EE  F       VCG DG TY  E  LR+ AC+ Q  + ++    C  GA
Sbjct: 1063 VDCHLRCTTEYRPVCGSDGKTYNNECHLMRVKCESKVDISIVFEADCESPPQAALETDDACTEACPFEEELV--CGTDGLTYTNDCYLEQKACLQSIP-LAVSYQGPCAEGGVQFEG----LEVEPS--------DCILRCTNEHRPVCGSDGRTYDNKCLLEMHNCESEE--NIEIIF----------------ENSCDSPPVSALESLNDCDAQCSEEPSF-------VCGTDGVTYINECILRKKACQEQIPLDISYQGSCASGA 1290          

HSP 4 Score: 72.0182 bits (175), Expect = 9.199e-13
Identity = 82/258 (31.78%), Postives = 114/258 (44.19%), Query Frame = 0
Query:  230 INKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC---------EEAHLCGSKGCP--FGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPE-CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVC----EEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            I  PVCGSD  TY NEC L    C  + +LTV S   C          E+  C SK CP  +   C    G++ K Y ++C ++  AC  +L +L++ S  NC         N +   +E + K+   CP+ CP+       PVCG+D  TY + CSL+A  C ++   D+ ++  G                +CD  A      EED    CS  C             VCG DG TY  E  L+  ACK Q ++VV
Sbjct: 1676 IFAPVCGSDGNTYGNECSLRAKACKDQSDLTVRSVGSCLTEESTQVPTESQPC-SKICPAIYAPVC----GSDGKTYSNKCTLKAKACTEQL-DLSVKSMGNC---------NLEENVNESADKN--GCPQICPTI----HAPVCGSDGNTYSNECSLKAKACKEQL--DLTIRREG----------------NCDLDATTAINFEEDD---CSKICSN-------IFAPVCGSDGNTYSNECRLKAKACKDQSKLVV 1884          

HSP 5 Score: 68.5514 bits (166), Expect = 1.024e-11
Identity = 87/364 (23.90%), Postives = 134/364 (36.81%), Query Frame = 0
Query:  233 PVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE-------------------EAHLCGSKGCPFGGSCVRTG----------------------------------------------GNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEK-PIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPE------GTSPNSANTSCEI 524
            P+CGSD++TY NEC+L   GC +R  L V    PCE                    A +CGS G  +   C+                                                 G++   Y ++C +   AC  +L +LT+ S  +C +         D      +G       +CP       +PVCG+D  TY + C+LRA  C+++   D+ V+  G+C        PI  G    D   +C                  P  +A  VCG DG TY  E +L+  AC+ Q ++ +A          ++GD    + +  LA+  +C     ICP       G+  N+    CE+
Sbjct:  342 PICGSDNVTYANECELEVTGCKERSQLEVAFAGPCEGRMTRDALSEDCPDDCPDISAPVCGSDGNTYPNECILRAKACAEESDLTLQTPGTCEDVQETTESISVEEEDCPQFCPDIFSPVCGSDGNNYSNECELRTKACTEQL-DLTVKSTGHCGT-------EVDATTDANNG-------DCPQKCTANFEPVCGSDGNTYSNECNLRAKACTEEL--DLTVRNQGLCNNEDTTTGPIEAG----DCLKIC------------------PAIHA-PVCGSDGNTYGNECNLKAKACEEQLDLTIA----------KLGDCLKDDSTQILAVTGDCPK---ICPAIFAAVCGSDGNTYTNECEL 652          

HSP 6 Score: 68.1662 bits (165), Expect = 1.234e-11
Identity = 61/249 (24.50%), Postives = 100/249 (40.16%), Query Frame = 0
Query:  232 KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHL---CGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            +PVCG++ +TY NEC L  + C     + ++ +  C +      C  K  P  G+       N + Y ++C + LDAC++R                   K    C   E+          CP+   +   P+CG+D ITY + C+L+A +C +      R+       T+    P P   +         +D   +CS                VCG DG+TY  E +L+  AC+   ++V+    PC
Sbjct: 1306 QPVCGTNGVTYPNECKLRLSACQSETIIRLLHEGECTDQECQEDCDKKLSPICGT-------NGQSYSNECLLNLDACQTRQAIQV--------------KHEGQCTPEEQ----------CPADCTDNYAPICGSDGITYSNECTLQAKSCLQSE----RI-------TVTQSGPCPSDTA---------DDCPAICST-----------IIAHVCGSDGKTYANECNLKAEACQTDLDLVLRSQGPC 1492          

HSP 7 Score: 67.0106 bits (162), Expect = 2.448e-11
Identity = 70/268 (26.12%), Postives = 113/268 (42.16%), Query Frame = 0
Query:  230 INKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCE---------EAHLCGSKGCP--FGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCR----TIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT 482
            I+ PVCGSD  TY NEC L    C ++++LT+  +  C+         E   C SK C   F   C    G++   Y ++C ++  AC+ +   +  SS          C+  +       + + + +CPE  +       PVCG+D   Y + C L+A  C K+   D+ V+  G C         E+P    + + +       +    C + C      P  +A  VCG DG TY  E +L+  AC+   ++   +   C   AT
Sbjct: 1794 IHAPVCGSDGNTYSNECSLKAKACKEQLDLTIRREGNCDLDATTAINFEEDDC-SKICSNIFAPVC----GSDGNTYSNECRLKAKACKDQSKLVVFSSGG--------CETEDSTNVPNNTNECITACPEIFA-------PVCGSDGFPYSNECELKAQACLKQM--DITVRNAGNCEPEDSIPDLEEPEVSTQGATETDDTTVPNKATDCPMIC------PAIFA-TVCGSDGNTYGNECELKAKACREGSDLTFKNVGDCEAEAT 2032          

HSP 8 Score: 66.2402 bits (160), Expect = 4.855e-11
Identity = 74/258 (28.68%), Postives = 109/258 (42.25%), Query Frame = 0
Query:  230 INKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC---EEAHLCGSKG-CP------FGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            I+ PVCGSD  TY NEC+L    C ++++LT+     C   +   +    G CP      F   C    G++   Y ++C +   AC  +L  LT++   NC +   T    +D     +         +C S       PVCG+D  TY + C L+   C ++  +D+RVK  G C            +S+   GA   ED   +C +  K             CG DG TY+ E  +R  AC  +  I VA   PC
Sbjct:  575 IHAPVCGSDGNTYGNECNLKAKACEEQLDLTIAKLGDCLKDDSTQILAVTGDCPKICPAIFAAVC----GSDGNTYTNECELRAKACIEQL-ELTVTGLGNCEADETTSIPVDDSTQVPELTD------DCNSGCIALYAPVCGSDGKTYSNECLLQRKACREE--SDLRVKSSGGCND---------NESTQVDGA---EDCPAICPLIFK-----------PTCGSDGNTYDNECKVRAQACFTKTNITVASEGPC 796          

HSP 9 Score: 65.4698 bits (158), Expect = 8.131e-11
Identity = 85/298 (28.52%), Postives = 115/298 (38.59%), Query Frame = 0
Query:  230 INKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC----------EEAHLCGS--KGCPF-GGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRG----------NDVRVKYPGV---------CRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEE-----QFPKYYA------------------GAVCGXDGRTYETEXDLRRHACKMQKEIVVA 472
            I KP CGSD  TY NEC +    C  + N+TV S+ PC          EE    GS    C F  G  V   G+NN+ Y S+C M  ++C ++L NLT+     C     T       +Y   S   L  CP           PVCG+D ++ D+ C L    C   +           +D  V   G+          +     +P+ C  +   F   CE D++     T  E E      FP   A                    VCG DG+TY  E  LRR AC +   IV++
Sbjct:  759 IFKPTCGSDGNTYDNECKVRAQACFTKTNITVASEGPCATVPEFHPILEEGSDSGSCDYSCDFITGIPVPVCGSNNETYGSECEMNKESCMNQL-NLTVQFEGTCDQLQVT-------VYQVGSDGCLFICPSLYR-------PVCGSDGLSRDNDCELERDICRSAKELYKISDGTCEDDNAVIIAGLETESENKCNLKCTNELRPV-CAANGQTFNNKCELDTFNCQHGTTFEVEFENDCDFPPLSALETESTSDCELSCSTNDVDPVCGTDGKTYTNECFLRRWACILGLPIVIS 1040          

HSP 10 Score: 64.3142 bits (155), Expect = 1.613e-10
Identity = 83/314 (26.43%), Postives = 117/314 (37.26%), Query Frame = 0
Query:  198 RHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEE---------------------AHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYS------------CSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRT-IKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPC 477
            R  NC   A+  ++ +  D    C  I CS  I  PVCGSD  TY NEC L    C  +  L V S   CE                      A +CGS G P               Y ++C ++  AC  ++ ++T+ +  NC               +Q     +D     K+      CP   +        VCG+D  TY + C L+A  C  + G+D+  K  G C      E P+  G    D   +C                  P  +A  VCG DG TY  E +L+  AC+ Q E+ V +   C
Sbjct: 1827 REGNCDLDATTAINFEEDD----CSKI-CS-NIFAPVCGSDGNTYSNECRLKAKACKDQSKLVVFSSGGCETEDSTNVPNNTNECITACPEIFAPVCGSDGFP---------------YSNECELKAQACLKQM-DITVRNAGNCEPEDSIPDLEEPEVSTQGATETDDTTVPNKATDCPMICPAIFAT-------VCGSDGNTYGNECELKAKAC--REGSDLTFKNVGDCEAEATTELPLLAG----DCPQIC------------------PAIFA-TVCGSDGNTYSNECELKAKACRDQSEVTVKNIGNC 2086          

HSP 11 Score: 64.3142 bits (155), Expect = 1.785e-10
Identity = 92/347 (26.51%), Postives = 136/347 (39.19%), Query Frame = 0
Query:  229 RINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPC-----EEAHLCGSKG---------CPFGGSCVRTG--GNNNKIYKSQCHMELDACESRLTNLTMS--------------------------------SHDNCYS--CS---------QTCKWNEDCLYSEKSGKSLCSCPE---CPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVC-------RTIKYEKPIPCG-------------KSSCDFGAVCE----------EDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATR 483
            +I  P+CGSD +TY NEC+L    CT++ +L+ +S+ PC     E + L   +G         C    S +     G++N  Y S CH++   C +    L MS                                S+ N YS  CS         QT  W  D   +  +     + PE   C  + +   +PVCG++N TY  +C L    C++ +  D+ ++Y GVC       ++ + +    CG               +C  G V E          EDS   CSVTC  E  F       +CG D  TY  E +L    CK + ++ VA   PC    TR
Sbjct:   47 KIRLPICGSDGLTYMNECELSLKACTEKSDLSKVSEGPCSDLSEENSTLIEGEGQDTDTDSAECILDCSPIFAPVCGSDNITYSSLCHLKHRECATGKQILEMSTGPCPTDSDLSVIDPTEECPDRCHTDFKLVCGSNGNTYSSECSMKAMGCKVNQTITWVHDGQCASNASDIHHATPEGGKCNCSVSFRFEPVCGSNNHTYPEICILYKEACARPQL-DLVIQYTGVCVHDTSCTQSCRQDYEPVCGSDANTYPNACTLQSEACKLGKVIEIVRQGPCDNREDS---CSVTC--EAVF-----SPICGSDNVTYANECELEVTGCKERSQLEVAFAGPCEGRMTR 382          

HSP 12 Score: 57.3806 bits (137), Expect = 2.381e-8
Identity = 68/260 (26.15%), Postives = 99/260 (38.08%), Query Frame = 0
Query:  232 KPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEA-------------HLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRT-------IKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVV 471
            +PVCGSD  TY NEC+L    CT+ ++LTV +   C                 +C +   P  GS   T GN   +    C  +LD   ++L                      DCL  + S + L    +CP         VCG+D  TY + C LRA  C ++   ++ V   G C         +     +P     C+ G +                      YA  VCG DG+TY  E  L+R AC+ + ++ V
Sbjct:  518 EPVCGSDGNTYSNECNLRAKACTEELDLTVRNQGLCNNEDTTTGPIEAGDCLKICPAIHAPVCGSDGNTYGNECNLKAKACEEQLDLTIAKLG---------------------DCL-KDDSTQILAVTGDCPKICPAIFAAVCGSDGNTYTNECELRAKACIEQL--ELTVTGLGNCEADETTSIPVDDSTQVPELTDDCNSGCI--------------------ALYA-PVCGSDGKTYSNECLLQRKACREESDLRV 732          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold965_size75845-processed-gene-0.0 (protein:Tk03764 transcript:snap_masked-scaffold965_size75845-processed-gene-0.0-mRNA-1 annotation:"PREDICTED: pikachurin")

HSP 1 Score: 69.707 bits (169), Expect = 2.398e-12
Identity = 57/250 (22.80%), Postives = 106/250 (42.40%), Query Frame = 0
Query:  551 CYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAG----TSIGFVGCIKELKINKKTISLQ-SMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG 795
            C C  G  G +C  D  ++   +  F+G  S++ +   T++   +++ + FR  + +  LLL G+S +++                   +   +     HI    D G+G   +++  ++   +W  L   R+     L+L       G ++G    +     + +GG             +N G    T+ GF GCI+ L+IN K  + + S R+   +      EC E++CS  PC+N G+C+S   G
Sbjct:  214 CLCPHGKYGEKCALDRYIS---SPRFSGH-SYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTG------------------DYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGT---------GSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGG 432          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1671_size31647-snap-gene-0.7 (protein:Tk04554 transcript:maker-scaffold1671_size31647-snap-gene-0.7-mRNA-1 annotation:"protein eyes shut")

HSP 1 Score: 65.0846 bits (157), Expect = 7.906e-11
Identity = 43/132 (32.58%), Postives = 61/132 (46.21%), Query Frame = 0
Query:  739 GFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQ--GFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKD--RGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSA 866
            GF GC+K L+IN   + L    +      R +VEC    C+  PC+N  +C        + C+C   FTG  C+R     CD NPC + G C       GF+C+C    Y G  C+     T   QPA+ ++
Sbjct:  839 GFQGCMKHLQINGLVVDLYQDAD----SGRDVVECRSAICALQPCQNGAICTEHETSGSWFCNCPPGFTGPLCERLV---CDTNPCHSGGTCMGSKVGPGFICLCPLG-YVGALCETSMNIT---QPAFTTS 959          

HSP 2 Score: 56.225 bits (134), Expect = 4.656e-8
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 0
Query:  781 NPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDLKTAD 855
            NPC N G+C++    ++C C   + G +C+    ++CD NPC+N+ +C + +  F C C   +Y+G  C  K  D
Sbjct:  102 NPCLNGGMCMNMEGSYICSCPFGYAGPNCEVEL-EKCDSNPCENDALCFLVEDEFHCYCV-PDYHGPRCQFKYDD 174          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold451_size166902-snap-gene-0.17 (protein:Tk01628 transcript:maker-scaffold451_size166902-snap-gene-0.17-mRNA-1 annotation:"neurogenic locus protein delta")

HSP 1 Score: 64.3142 bits (155), Expect = 1.182e-10
Identity = 31/69 (44.93%), Postives = 39/69 (56.52%), Query Frame = 0
Query:  773 CEEN--SCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            CE N   C+GNPC+N G C   V  + C+C   F G++CQR  +D C  NPC N G CH     F+C C
Sbjct:  109 CEVNVDECAGNPCRNGGTCFDFVNDYKCYCHPGFVGRNCQRNVDD-CTTNPCANGGTCHDSINDFICTC 176          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold552_size138156-snap-gene-0.26 (protein:Tk00651 transcript:maker-scaffold552_size138156-snap-gene-0.26-mRNA-1 annotation:"fat-like cadherin-related tumor suppressor homolog isoform x2")

HSP 1 Score: 58.9214 bits (141), Expect = 9.070e-9
Identity = 58/244 (23.77%), Postives = 92/244 (37.70%), Query Frame = 0
Query:  653 FSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQG-HLWIGGVDPNDFPLMYKVRKNAGTSI--GFVGCIKELKINKKTISLQSMREPLVIRRRGLVECE--------ENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRR------------------RND------------------QCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYC 849
            F++  G G+VTL     I  G+W  +  +R+     + L+   +  G+  G    LNL+   ++ G              +  G  I  GF GC+ +++++  ++ LQ   E  V + +     E           C  +PC N G C      + C C   FTG +C+                    +ND                   C+PNPCQ+ G+C    R  +C C G  Y GT C
Sbjct: 3282 FNFGSGEGSVTLNG-APINDGEWHTVKLERHGNSAKISLDNRFEEQGAAPGVNDILNLEDTSVYFGA-------------EVVGQDIAKGFEGCLDDIRLDGISLPLQLKDESQVAKLKRFNNVEFKCGQLQPPGLCGSHPCSNGGSCAEDEVTYTCSCLSRFTGLNCENDLDPCGSNPCLYGAKCVNLKNDFHCECPPQLSGKRCHYGQYCNPNPCQHGGICEEGTRSAICKCRG--YSGTNC 3509          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold90_size386344-snap-gene-1.16 (protein:Tk12044 transcript:maker-scaffold90_size386344-snap-gene-1.16-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_20079")

HSP 1 Score: 57.3806 bits (137), Expect = 2.405e-8
Identity = 50/169 (29.59%), Postives = 78/169 (46.15%), Query Frame = 0
Query:  649 KHIIFSYDLGSGAVTLK-SHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPL-MYKVRKNAGTSIGFVGCIKELKINKKTISL---QSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHC-KRDFTGKSCQR 811
            KHI    DLGSGA+ L+ S+ +++ G W  +   R  + G + ++        T G    L L G L++GG+D +  P  +  V   A    G+VGC+++L IN  T+ +    S ++   IR           C   PC   G C      ++C C K  FTG +C +
Sbjct:  322 KHI----DLGSGAIKLRASNRRLDDGSWHKVEILRNQRSGTISVDDELHSF-ETPGEAFKLELDGPLYVGGLDHSLEPFRVPPVLWTASLRKGYVGCLRDLDINGYTVDIAQFASKQDSGSIRSS--CHILPPKCPEKPCLQGGSCSEGWNRYICDCSKTSFTGSTCGK 483          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold369_size193746-snap-gene-0.33 (protein:Tk07567 transcript:maker-scaffold369_size193746-snap-gene-0.33-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_54646")

HSP 1 Score: 56.6102 bits (135), Expect = 2.556e-8
Identity = 66/249 (26.51%), Postives = 94/249 (37.75%), Query Frame = 0
Query:  229 RINKPVCGSDHITYKNECDLLQAGCTKRI---------NLTVISDLPCE-----EAHLCGSKGCPFGGSCVRTGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHAC 463
            RI++  CGSD   Y N C +L+  C K +         N    +D P +     E  +CGS G                IY+++C ++   C   LT   +           T K +      + +      CPE        ++PVCG D ITYDS C LR +TC+    + V+  + G C  +   KP  C K                   TC      P   A  VCG DG  Y +E  ++R  C
Sbjct:  199 RISRLTCGSDGKLYNNGCQMLRKNCGKHVYDVPVPFCLNQLYRTDCPSDCSTQAEDLVCGSDG---------------NIYRNECELKKMTCGFPLTRYEL-----------TVKVDMSLCLDKLANCDKMVCPE-------AQEPVCGNDGITYDSPCFLRKATCT----SGVQTSHEGPCADL--SKPDECPK-------------------TCP-----PSTPADIVCGSDGNAYSSECTMKRETC 384          
BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold287_size221780-snap-gene-1.29 (protein:Tk01903 transcript:maker-scaffold287_size221780-snap-gene-1.29-mRNA-1 annotation:"neurogenic locus notch protein isoform x1")

HSP 1 Score: 56.6102 bits (135), Expect = 3.670e-8
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 0
Query:  770 LVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYC 849
            + EC+E    G+PC+++G C++    + C C R FTG  C+   N +CD NPCQN G C  +  GF C+C    Y G +C
Sbjct:  421 INECDE----GSPCEHDGKCVNTPGSYRCDCARGFTGPRCEVNIN-ECDSNPCQNEGTCIDERGGFRCICM-PGYNGRHC 494          

HSP 2 Score: 55.4546 bits (132), Expect = 9.139e-8
Identity = 27/64 (42.19%), Postives = 35/64 (54.69%), Query Frame = 0
Query:  776 NSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            N C  NPC+NEG CI +  GF C C   + G+ CQ    D+C  NPC+N G+C      + C C
Sbjct:  461 NECDSNPCQNEGTCIDERGGFRCICMPGYNGRHCQTDE-DECASNPCRNGGICTDLINSYRCTC 523          

HSP 3 Score: 53.1434 bits (126), Expect = 5.296e-7
Identity = 28/66 (42.42%), Postives = 35/66 (53.03%), Query Frame = 0
Query:  774 EENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVC 839
            E + C   PC+N   C   +  + C C   F GK+C+   +D C PNPCQNNGVCH     F C C
Sbjct: 1153 EVDECLSQPCQNGATCKDLIGTYQCMCPPGFQGKNCEFNIDD-CTPNPCQNNGVCHDLVNDFRCSC 1217          

HSP 4 Score: 52.373 bits (124), Expect = 9.017e-7
Identity = 36/91 (39.56%), Postives = 44/91 (48.35%), Query Frame = 0
Query:  767 RRGLV----ECEENSCSGNPCKNEGL--CISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHIKDRGFVCVCSGSEYYGTYCDL 851
            R G V    E + N C  NPC   G   CI  V  + C CK  + G+ C+ +RN  C  +PCQN GVC     G  CVC    +YGT C  
Sbjct: 1256 RPGFVGPRCEGDVNECLSNPCSPLGTKECIQVVNNYQCSCKPGWQGRHCETKRN-FCQHSPCQNGGVCTNTQTGHQCVCPHG-FYGTNCQF 1344          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000004167 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 25
Match NameE-valueIdentityDescription
-4.353e-3128.83symbol:Agrn "agrin" species:10090 "Mus musculus" [... [more]
-1.879e-2828.31symbol:Agrn "Agrin" species:10116 "Rattus norvegic... [more]
-1.944e-2828.31symbol:Agrn "Agrin" species:10116 "Rattus norvegic... [more]
-1.944e-2828.31symbol:Agrn "agrin" species:10116 "Rattus norvegic... [more]
-2.145e-2827.89symbol:AGRN "Agrin" species:9031 "Gallus gallus" [... [more]
-2.273e-2828.53symbol:AGRN "Agrin" species:9031 "Gallus gallus" [... [more]
-5.018e-2728.20symbol:AGRN "Uncharacterized protein" species:9913... [more]
-1.155e-2527.09symbol:HSPG2 "Uncharacterized protein" species:991... [more]
-1.899e-2528.16symbol:AGRN "Agrin" species:9031 "Gallus gallus" [... [more]
-1.971e-2529.10symbol:AGRN "Uncharacterized protein" species:9615... [more]

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BLAST of EMLSAG00000004167 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592842438|gb|GAXK01115106.1|5.019e-5736.68TSA: Calanus finmarchicus comp1152792_c2_seq1 tran... [more]
gi|592814217|gb|GAXK01140351.1|3.686e-4428.72TSA: Calanus finmarchicus comp817742_c1_seq1 trans... [more]
gi|592814216|gb|GAXK01140352.1|2.530e-4332.73TSA: Calanus finmarchicus comp817742_c1_seq2 trans... [more]
gi|592829622|gb|GAXK01127922.1|5.256e-3630.74TSA: Calanus finmarchicus comp875548_c0_seq1 trans... [more]
gi|592749933|gb|GAXK01204480.1|3.789e-3330.37TSA: Calanus finmarchicus comp206836_c0_seq4 trans... [more]
gi|592749935|gb|GAXK01204478.1|9.267e-3329.84TSA: Calanus finmarchicus comp206836_c0_seq2 trans... [more]
gi|592749934|gb|GAXK01204479.1|1.205e-2830.17TSA: Calanus finmarchicus comp206836_c0_seq3 trans... [more]
gi|592749936|gb|GAXK01204477.1|2.130e-2830.17TSA: Calanus finmarchicus comp206836_c0_seq1 trans... [more]
gi|592750032|gb|GAXK01204381.1|6.116e-2625.41TSA: Calanus finmarchicus comp207306_c0_seq1 trans... [more]
gi|592750031|gb|GAXK01204382.1|6.166e-2625.41TSA: Calanus finmarchicus comp207306_c0_seq2 trans... [more]

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BLAST of EMLSAG00000004167 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 16
Match NameE-valueIdentityDescription
EMLSAP000000041670.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s220:20338... [more]
EMLSAP000000039939.366e-2324.63pep:novel supercontig:LSalAtl2s:LSalAtl2s213:44113... [more]
EMLSAP000000123665.331e-1727.79pep:novel supercontig:LSalAtl2s:LSalAtl2s903:98980... [more]
EMLSAP000000074986.104e-1326.50pep:novel supercontig:LSalAtl2s:LSalAtl2s430:28805... [more]
EMLSAP000000031847.527e-1226.32pep:novel supercontig:LSalAtl2s:LSalAtl2s176:57537... [more]
EMLSAP000000031153.180e-1129.17pep:novel supercontig:LSalAtl2s:LSalAtl2s174:63882... [more]
EMLSAP000000005513.517e-1126.56pep:novel supercontig:LSalAtl2s:LSalAtl2s108:38311... [more]
EMLSAP000000053532.269e-921.88pep:novel supercontig:LSalAtl2s:LSalAtl2s289:18498... [more]
EMLSAP000000034351.612e-843.24pep:novel supercontig:LSalAtl2s:LSalAtl2s188:58119... [more]
EMLSAP000000123521.628e-832.08pep:novel supercontig:LSalAtl2s:LSalAtl2s903:17685... [more]

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BLAST of EMLSAG00000004167 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE3.412e-3128.83RecName: Full=Agrin; Contains: RecName: Full=Agrin... [more]
gi|399021|sp|P25304.2|AGRIN_RAT1.525e-2828.31RecName: Full=Agrin; Contains: RecName: Full=Agrin... [more]
gi|2494281|sp|Q90404.1|AGRIN_DIPOM6.412e-2826.64RecName: Full=Agrin[more]
gi|460018313|sp|P31696.3|AGRIN_CHICK1.490e-2528.16RecName: Full=Agrin; Contains: RecName: Full=Agrin... [more]
gi|1172451|sp|Q05793.1|PGBM_MOUSE2.759e-2527.17RecName: Full=Basement membrane-specific heparan s... [more]
gi|460018339|sp|O00468.5|AGRIN_HUMAN6.212e-2427.27RecName: Full=Agrin; Contains: RecName: Full=Agrin... [more]
gi|317373536|sp|P98160.4|PGBM_HUMAN3.407e-2327.38RecName: Full=Basement membrane-specific heparan s... [more]
gi|221271989|sp|B4F785.1|EGFLA_RAT6.076e-1927.02RecName: Full=Pikachurin; AltName: Full=EGF-like, ... [more]
gi|158705693|sp|A3KN33.1|EGFLA_BOVIN1.023e-1826.48RecName: Full=Pikachurin; AltName: Full=EGF-like, ... [more]
gi|121949534|sp|A0A1F4.1|EYS_DROME1.612e-1825.52RecName: Full=Protein eyes shut; AltName: Full=Pro... [more]

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BLAST of EMLSAG00000004167 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 25
Match NameE-valueIdentityDescription
gb|EFA11111.1|1.974e-4528.26hypothetical protein TcasGA2_TC004709 [Tribolium c... [more]
XP_016770916.13.217e-3828.74PREDICTED: agrin-like isoform X4 [Apis mellifera][more]
XP_006566972.13.397e-3828.74PREDICTED: agrin-like isoform X7 [Apis mellifera][more]
XP_006566973.13.417e-3828.74PREDICTED: agrin-like isoform X8 [Apis mellifera][more]
XP_006566971.13.680e-3828.74PREDICTED: agrin-like isoform X6 [Apis mellifera][more]
XP_006566970.13.786e-3828.74PREDICTED: agrin-like isoform X5 [Apis mellifera][more]
XP_006566966.13.824e-3828.74PREDICTED: agrin-like isoform X1 [Apis mellifera][more]
XP_016770915.13.831e-3828.74PREDICTED: agrin-like isoform X3 [Apis mellifera][more]
XP_016770914.13.916e-3828.74PREDICTED: agrin-like isoform X2 [Apis mellifera][more]
XP_016770917.15.384e-3828.74PREDICTED: agrin-like isoform X9 [Apis mellifera][more]

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BLAST of EMLSAG00000004167 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 25
Match NameE-valueIdentityDescription
gi|1101350313|ref|XP_018901101.1|1.498e-4830.45PREDICTED: agrin-like isoform X3 [Bemisia tabaci][more]
gi|1126207770|ref|XP_019637523.1|1.790e-4827.12PREDICTED: agrin-like isoform X5 [Branchiostoma be... [more]
gi|1080047455|ref|XP_018565030.1|1.655e-4728.03PREDICTED: agrin-like [Anoplophora glabripennis][more]
gi|1126207762|ref|XP_019637519.1|1.915e-4726.78PREDICTED: agrin-like isoform X1 [Branchiostoma be... [more]
gi|1126207776|ref|XP_019637526.1|2.132e-4727.00PREDICTED: agrin-like isoform X8 [Branchiostoma be... [more]
gi|1126207766|ref|XP_019637521.1|2.336e-4726.78PREDICTED: agrin-like isoform X3 [Branchiostoma be... [more]
gi|1126207774|ref|XP_019637525.1|2.703e-4726.78PREDICTED: agrin-like isoform X7 [Branchiostoma be... [more]
gi|1126207768|ref|XP_019637522.1|3.201e-4726.78PREDICTED: agrin-like isoform X4 [Branchiostoma be... [more]
gi|1126207772|ref|XP_019637524.1|3.547e-4726.90PREDICTED: agrin-like isoform X6 [Branchiostoma be... [more]
gi|1126207764|ref|XP_019637520.1|3.660e-4726.78PREDICTED: agrin-like isoform X2 [Branchiostoma be... [more]

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BLAST of EMLSAG00000004167 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 15
Match NameE-valueIdentityDescription
maker-scaffold421_size176100-snap-gene-0.221.024e-12031.90protein:Tk10554 transcript:maker-scaffold421_size1... [more]
maker-scaffold48_size466083-snap-gene-1.191.015e-2925.53protein:Tk05968 transcript:maker-scaffold48_size46... [more]
maker-scaffold155_size301336-snap-gene-1.52.943e-1628.52protein:Tk03781 transcript:maker-scaffold155_size3... [more]
snap_masked-scaffold965_size75845-processed-gene-0.02.398e-1222.80protein:Tk03764 transcript:snap_masked-scaffold965... [more]
maker-scaffold1671_size31647-snap-gene-0.77.906e-1132.58protein:Tk04554 transcript:maker-scaffold1671_size... [more]
maker-scaffold451_size166902-snap-gene-0.171.182e-1044.93protein:Tk01628 transcript:maker-scaffold451_size1... [more]
maker-scaffold552_size138156-snap-gene-0.269.070e-923.77protein:Tk00651 transcript:maker-scaffold552_size1... [more]
maker-scaffold90_size386344-snap-gene-1.162.405e-829.59protein:Tk12044 transcript:maker-scaffold90_size38... [more]
maker-scaffold369_size193746-snap-gene-0.332.556e-826.51protein:Tk07567 transcript:maker-scaffold369_size1... [more]
maker-scaffold287_size221780-snap-gene-1.293.670e-840.00protein:Tk01903 transcript:maker-scaffold287_size2... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s220supercontigLSalAtl2s220:203389..278765 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s220-augustus-gene-2.5
Biotypeprotein_coding
EvidenceIEA
NoteAgrin
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000004167 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000004167EMLSAT00000004167-700014Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s220:203389..278765+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000004167-686933 ID=EMLSAG00000004167-686933|Name=EMLSAG00000004167|organism=Lepeophtheirus salmonis|type=gene|length=75377bp|location=Sequence derived from alignment at LSalAtl2s220:203389..278765+ (Lepeophtheirus salmonis)
TAACAACCATATTATTTCTTTAAATCTTAGTTTTTCATGCAACTTCAACA AGTGAATAGCTTAGGCTTCATGTGCACCATATTTCTTATGCTATGAAATC ATCAAAGTCAACAAAAGTCATCACTCCACGCAACGTGGACCTTCCCCCCC ATGTCCTCTCCAAAGACTTTGCCTCTGGACCTCAAGGACCCTTTCCTCGC AAAAAATGTCTTTGTCTCTCTCTTTGGCTACTGGGACTCCTACTTTTATG TATTGGAGCCGTTGCGGCTCTTAACTTTGTACTCCCCGACGTACTACTAA GTTCCAATGGAGGAGTTCCTGAAGTTTATGGACTTATGCGTTCTGAAGAA TGTCCAAATGAAGATGGATCATCTCTCGACTACCGTGTCTCTGCATCAGA TGTCATTCTTGTGGGCTCAAAGAGCCCACTTTTATCAGGAGAAGAAGACG AAGGAGGTCTTCGAGCAGAGAGGGTTTTAAAGGGTTCTCTTATGAGGAGG GAGCTATTCGGAGGTCCATGTGCCGTGAGTTTGAGTGAAGGGAAACGTGT ATTTTTCTTGGAGCATGTGGATGAGAGTCTACGGCTCAAATTTCCCAGTT TGCTTGCATCAGATAAGTTGATTCGAGTGATTCGGAAACTTTTGGAGACG GAAGGTAAGGACGTTATTTTTTTTATGTTTAGACATATCAAATTAAAAAT GAAAAGTATTTTCATATATATCTCTCTTGGGCTGTATGCTCAAATAAATG ACGAAACTCCACCCATACTCATTTCGTTCCAAAAAAATAGTTATTCACTA AATACATATGCTATAGGGGATCTTTTTATCAGTGAGAGGGAAGTTCACAA TCCAAGATATAAATGTACACAACATTCGACTATCCTCTAGACTTCATTTC CTTTATGACACCCACTTTCCGTTCTGACTAATTTCACTTAATAAGATAAA TAAATGACCCAAGGAACATTTTCCTATATTTTTCTTCAGACCACAGAGGG AAAACATGTGGTTTTATTATTTGTATATCTGTGTGTGATACCATCAAAAT AGGGGCAGGGGGAAGAGGAAAAGGGACTAGATGTTAAAGAGGGTGGAGGG GCAGAATTGCAAACCATAAATAATAATAATAATCATCAGGGGAGAAAAGG ATTCAACAACAGGTGTACGTATCTATTTAACCTCCATGAATATGTGTACA GTACTCCCAAACCTCAATTGAACTCATTACGCTTTTGACTTATATAGGTA CCTATTGATTTATATGTTCAATTAAATTCTCAATAAGGTTATTCATGTGA CATCAGCATTTTTGAGAGCAGGGATTTTGAAAGCATGAAAAACCAAGTTT ATAAAAAAAAAAACATCCTTTTTCACTGAGATTTTAAGGGAAATGGGGTA TTTGCATGTCAAAATAGGACCGACAAATAAAATTAATTAAGTCATTACTG TCTTCCAAAAATAAATATGTCCTTCAAATGACTAGTTGTATTATATGTAT GACCTTAATAGTAATATGAATTAAAATTATGTTGTTATATTGTCTTATTT AAATTGTGTAGACAATAAAATTAACTTTTACATTTGCAAGGATATTGATT TGCGCCCAATTACATTACTTTTAGCATCGGTAATCCATATAAAAGATAAT TAATTTTACAAATCTAGCCAATTCATAGGGATTTTATCATCCATTATGTG ATTTGATTCACTGATTCTTTTTTTTTTTCTTTTTTTTTTTGGCATGAAGT AATATTTCAATAAAGATGAGTATTTTTTCATTTTAAATGCCAATTTTTTC AATATTTTTTAGGAAAACTTGTCATATTATTTATAAAATACATGGGGTTG CTTTTAGTTAATGATGCAATCCATGACGTCAGTGACAAGCATATATACCA ATTAGTATTGTCACGATCCTAATACTTTAGTATAAAGCATAGTACCAAAA TTAGTATCGGTATTGCGATATTTTTGTTATTTCATCGACTAAAAATATTT AAAAAAAAACAAACAATGTCGTTACAAACAATTTAAAGGTGTGTTCGCAT GATTTTGGTTTTAGGAGAGGGAACAAGCTCAATTTGATAATTTTGTGTGT TGAGAGTAAGGCCATCAGTTGGCCGATATTTTTTAGCATAATGGTACAAA AAAATACCGGTTTCACTACTAATTTAGTATAATACTGTTCTAATTGTGGC AGTACTCTAATAATTCAATTTATAGCAGGGATTCTCAACCTTTTTTTTTT GTAAGGCATGTAAGATCATTAAACATTGCAACTGAAAATATACTGCATTT TTCTCAATATATATATATAAAATAACTTAAGATCTTTAAAACAAACTTAT TTATAAAAAAAAATATTTTTCAAGTACCCCAAGGGGTATGCGTTCCCCCA TTTGAGAACCACTATCCTATACCATGGCTTTACTGATTAACTCTGAAAAA ACAACATTAAAAAACTCTGCAAAACTGAAGACTTTTTCTAAAATAGATAT ATTGAGATTTACATCTTGAAATAAAACAAAACTATAATTATTTAAAAAAA TATTTCTNAGAGCTGTCTCTCCTTTGCCAATTTTGAGAGGATTTAGCGCC CACATATGTCTTTCAGGTAGAATTAATAGCAGTAGTAGAGGTACATGTTA TATTCATAGTAGTCATAGACAGTAGAAGAAGTTTTTAGACCCTATATATT ATGTCTTTGGAATTATTTGTTTTATCTACGATATACATGCATATATATGG AGTAATTAGCAATTATTACAAGTGGTTCTTACCCACCTACAAATCCTGCA TTCCTATTTTAAAATTTTAAAAAAGTTATATATATATTTGTAAATATGTA AGGTAAACTTATGGGGTAAGTTGCAACACAATGGCTCAAAGTTAGTTTTA CTAAACAGGACAAAATTTAAGACAGTATTTTTACATATATCTGCTATTTT TTTAATAAATATATTAGTTTTTCTTTAGATTATAAGTGAATGCAACCTTT AAAATTTATTATGTTTATAATTATTAGTTTAGAATAGGGTTCAAATTTTT ATCCAACTCGAAAAGATACTCGATTTACTAATTTGAACAAATATTGTTTA AACTAATCCAACTCGCTCATTTTTTTTTTTTTAAGGGAAAATTATACTTT ACTAGAATAATCAAAGCTCAAAACATTTAAACAATAATGACGATTAAAAA AATATTACAGACGCTTCTTTCTTATTATTGAAAGAGGAGCTATTGATGAC CCATAAAAAAGGAGAAACAACTATGTTGCATAATGTGCAAAAATTCTCAA AAATAAATAAAATTACACTCAAATTGAAAATTGATATATTGTCCTAACAC AAACTCAACATGATGCATACCTCAAAAGCATTGAATTTACACTAGTTACA ACTCTCCTGGTCTACCCTACTTTATTTATTATCGTTCAAATATGATGTTT TACTGTCCTTTCGAATGATAGTAGTAGAAATGTGACCCATCATTACTTCA ATGTCTTGAGTTTAATTAATGTCGATTATACAATATAAAATAAAAAAACG TGATTTCGAATAATTTTCAGAAAATAGTCGGGTTTAGTAGTAAGTCGAGA TAAACTCAAAACCAATACTGCAACTGTCTCCGGTCTCCCCATCTAGTTAT CTTTCAAATCTGAATTGAGGGTGAAATCCTTAATTAATTATTATGATCCA CTGGTGCAAATGGTTGTACATATGTCCATAACAAAAAAAAATTTGTAAGG CCCAAGCACTCAATACTCAGACATCCCCAAAAACCATGGAATGAAGTGTA ACCCTTATTCAGATTGTAACGAATCAAAATTTGAAATCCGTTTGATGTTT AACAATAGAATTATAATTAACTTTGGAGAGTTGCTTTTATACGTACCTAG GTATATATACAAATAATAATAATATTTCTTTTTCTAATTTTTTTGATGGT TGTAGAGGTAGAATGGAATATATGTAAAAGTGTAGGCGATACAAATTCAT TCCTTTCTCTCTATCTCTTCAAAAATGATATATCATCCTAATAATAATTC AAATAAAAAAGAAACAACATTAAACAAAAAATAATTGTAATTTCTCTTTA TAAGTAACCATTTGAGGGAAGGAGGAGGGAAATGGTTTTATATGCATGTA TTTATATTAGAATGTACCCTTTTTGGGTTAACATCCGCCCACCCACATAA GTAACTTTTCTGCTGGAAATAATAATAAAAAAATGACTACCTCAAAGATT GAGGCCTATTAACACATATAGTTTAAAAAAATAGGATATCTTGAGGTCAA AATTAGCCATTTCAGGTCATTTGTACCTCTTGTGCTAAATATGATGCTAT GAGTCAAAAGGTATGTTTGTTTTTATTTATATTAGTATTTGATTTATCAT TAATAAAAGTATTTTAGGCCGTTTACAGGCAAAACATGGAAAATATAAGT AAAAAATTGTACTTTTATCTATTTTTTTTTTCTAATTAGTTTATAAAATT GGAAATATTATGCACCTTTTCTTCAATAATTTTAGGGCTGTAAGATGATT TACTCAATGAGGATTCCTAACATCCAATTTGAAACTTTACAAGAAGCCTA AAAATGGACAATTATGACTCAAAATTCTTATTTAAAAAAATTAAAATACC CTATACCACCCAAAAATAACATGAGTCCGTATTTTGATAAACACCCTGTA AAAAAAATGAGTTATATTTTTACAGCTATCAATTTAAATTTGTTTAAAGT ATGGTTTATAATATTAGATTGAATATAAATAACTGCAAATTATTTAGCAC ATTTTAGAGTCAATATATTATCAAAACAAAATGAAAAATATTTTTACTGA TTAGTCAAATACTAAAAAACATGTAAAGTATCATTTTTAACTCACAGACT TTTATTTGGGAATACAGAAGAGGGCAGACTATGTTTAATTTTTACCCCAA AATCTTATTTTTTTCAAGTTTCAATAACATGAGAGGCCTCTTACTTTGCT TAAGTTAGTTTTTGATAGCCACAGTAGGAAAAGTTAAAGCTAAATAGTGT TAAATTTTGCTATATTATTGGTAATAAAACAGGAAAACTGTCAATGGTTG AGCACATCTTAGACCTCATAAATTATTTGAAACAAAATGTACAATATACA TATTGATTGATTAACCGAATACTACGAAATATAAAAAGTACAATGTTTGA CTCAGTCATATATTTATTATTGTACAAAATGCCTGAATATAATCACAAAA ATGACTTTAATTTAAAAGTTAAATTGTCGTGTACTTCGCTAAAAAATGAC GTGAGAGGCTTCAAACTTTCTTAAAATCAGTTTTTTATAGTCCTCAGCAG AAAAAGTCTTTCTGCAGAGGAAACAAAATATTTACCAAAAAACGGAACAG TCTAATGTATATACATAATATAACTATAAATAAACATGGTTGATAAGTAT ACAAACCATGATTGTTAATTATTCACAATAAGAATAATTCATCTCCAAAG AGGAAAAAGGTTTGTGTCATTGAGATTAAAGAAATAAAGAGGTGGCGTGG GTGAGTGAGTGAATGTCTCTCTCTCTCTTACTTTTGTCATTCAAACAGCA ATACCAATTTGTTCTTTTCTTTATTTTTTTACAGATCTATATTTTAAATG CAAATGCCCTTCTCTCGTCATTCATTCCTTCACCATTTTTAAGCATGTGA ATAATAAATATACTCCACACAAGTTGTAACTTGTATAATTAGATTGTACA AGTTCCAACCAAAAATGAGATAAATATTTAAAGTAATTTTGATTGCAAAG GCGTAGGAGAGGTGCATTTAGGCYAATCTGGGATAATTCATTGTTTTTAA TATGAAAGAAAAACAAAAAACAAACATTCTTTTCCCTCCAAAAGATACGT TCAATATACTATGTATAAAAACGTCATTAAAAATTTAAACTGCCCTAAAA AAGTTCAAATGCCATTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNAGTTACTACATATGTGTCTTGTGAATATTGAATGAATA AGTAACTTAAGAAAATAATATCGAGAAATAGTTGTTAAATAGTTGCCCCT GCAAAATACCTGATAGACAGAATTGTCCTCGAATGCAAAAATTTGTTTCA ATCTTTTGTTTCTGGGCCTGTGCTCGATTCAGGGCACCAATAGAGGCCGK TGTAGAGGATATTGGAGACTACAATTATATATATTCTTAATGATTGTATC GGATGCATTGTTTTAGCTTTTTTTAATATATCTATAAAAATGACCATTTA ATTTGAATTCAAAGTAGATAGATCAAACGTGCCGGATTTCATTGTATATA CAAGTCAAAGATTTATTCATATTAGAAACAAAAARAAAAARAAAARAAAA ATAACATTGAGAAAAAATAGAATAAGTCTTGACATGAGTAGATAATGGAA GACCAATAGGTACATAATAATATTAATATAATGGACCTCACACGAACCAA CAACCACAGCCCACCACTTCATAAATAAATAAATACTAATAAAATATTGA GTAGTAGTGCATTGAAAAGTGTATGTGGGCAAAAAAAAAAAATACATTTT TGGGTAAAAATACATAAAGCACTTTAGATAGATGAAGTCACTTAAAAAGG CATATTTCTTAGCTACATTAGTACATGTGTGATGATGTAGGCTTTTCTTC AAAAATATATAAGTACATATTAGTGAATCACGGTTGGAGGCTAAACCATT GGGACAAGGCGTGTTATGTATTTTAAACCAACAATTTTAACAAGAATCCG TTTATTTATTAGTTTGTAGTTTTCTTTTTACTTGACCAATTAAATGTCCT AGTTAATATTATGCAACAATAGTGTCCCTTTTCTAGGGCTATCAAGCCAC AATCAAAAGGCTAGAAACTTAAGCAAATTCATGCTTTGCGGAATTTGGAA ACTAACATTGAAAGTATGGTTGAAGGTTTTCCTGTTTATGAGACAAGATA TTATATAACTTGCGGTGGAATATAAATTTTTTTGACTTGACAAACTGAAG AGGGGATTTTCTTTTCCTAAGTTGTTGTCATTATTATTTGATTCCTGAAA ATTGAACTTCATTTTCTACTACATACAATATTATGATTGATCATGGAATT CTATAATTGCATAATTTTTATTCCGGCTTGAGGGGAATATACAACGATGT GATTGGTTGAAAAAAAGGAAAAAGAAACCGTCAGACCTACTTTTTACTCC ATTCCTTCAAAAAAGGATCGAAATAGATACAAACTCGCTGTATATGTGAT TATTCCATGTGGCTAGAATTCCATACATTGATATTTACGAAACCGGATTA AACACTTGCGTGTGCTCATAAAAGAACCTAGGGATTTATTTCAGAGTTAA AATAGTAAGTCCTTTTCGAACTCAGAGTAAAATTTAGGATTGACATCGGA GTAAGTCTTTTCTATATTCTTGTTCATTTTCTTATCCCCCTCGTCACTGC TGCTTGTAGCTAATTTTATGAAATCTCATGTCAGCTAAGCTGCTCTTTGC ACAAATATTTTTCAAATTGTTGAGGTCACCATGTTGATTGTATGTTTTCT TTTTGTAACATGTCAAAAATGTGTGGCAAGAAATAATATATGATGATTTT TTGTACAACACAATTTTGATAAAGTGTTGGGGGCTGGGTTACATAATTCA GCTAATTTCACTAATACATGCATGCATAATTTAGTTAGATATATTGAGCC ACTTAAAAAGGCATACCTCCTAGCTCCATGAATACGAGCTATGATGATAG CTTTCCCCCTTCACAAATTATATGTGTATATTTGTATGGGTAATGGGTAT AATACAATTTATTTTCTTCTTTTTATTCAAAGGGAGTTGTTGATATCATG AAACTTTTTCTAATAAATGAAGGGGTGTGCTTCAAACATGGTTTATTTTT TTGGTTGTTGTTTCTWTTGTTGGGGGGGGGGGGAGTTYTTTKTATAAGAT GTATTTAGGGTTGTTCGTATTTACATATTTAGGATGTATAATGTATTTAT GATGATGATAATACTCAACTGGGCGAATGTTTCTCTTGTTTTGTTTTTTA GAGAATGAAAAATAATTCAATTTCATTATAAATCAAATTTCCATCTATTA AAAAACCTTATAGGGTAAAAATTCGGCATGTGTGTAGACCAGGGAATTTA TGGCAGAGGGGCAAGGGGKTGTATATCCCTTTGCAAAATCTTTCCAATCT GAAATATTTTCTGATGCTATTATACTAAAATGGTTTTTTAGTAGTTTGCT GTTATTAAAAATTGCTTTGTTTTTTTTTCTTCAGTACAGAGTGGACACCT AAAACTTGCAGCAGTATTTAATTACAATTTCATACCCATTATTTTTCGTT AGAAGGTTTCATTATAGAACCAAATGAGTATTGAAATACAAATAAACTAG TTTTGTTTTTATTTCATGTCTCTAAGTGTAAAAATGTCGGGGAAAAGGCT AACGTGTGCGATCTCCTCTGAACAAATTTAAGTGATGTTGTAGAGTCTCC TGGCATCTACATCCAGATTGCCTACCATGTCAATAAGTCCATTAAACCCC CATATGGCCAAGAACAACCAAAACTTCAGCAACAACGATACGGCTGRCAT CTGGCCTGCCTTCATTTGGCCCTCATCAGCCCTAAACTCAACCACTGCAC TTTGCAGTTTAAGGTATCTTGGAGATGAATGTCTGCCACAATACCTCATT TCCAAATTTGGATAGGCTCCGAGTTGCCATAATGACAACGTAGTACACTC TCGACACAACCTTTATCGTCAAGATTAAACAATTTGTTCGCTGGCATGTC GAGGCTGTTATTATTTTTGAAGGAAGACATATTGAATAAAAATAAAGCTA ATAACTACAGCATCAAAATATTTCACAAATTGGTTTATTTATTGTCTTTT ATGGATTCCATACAGATCACTTTTCCGTAATGTAGTCATAATTTTGGGTC CTCACTCTGAACAGAGAAACTAAAATAAAGTATCAATGAATTGAAGACAG TAATCTGTCATATTTATGATTCTTTTTTTCAAAAATTTGTTGAGTGGGTT TCCACCCATTAGTTCGGAAATACATAAAAAATATTTCACGGAGAAGTATA TTTTAGGAAAGGMAAATTGAGAGAAGCAAGATTCATCTTTTTNTTTATAA AAAGAAAAAAAAAAAAAAAAAAAAAAACAAAGATCCAAAAWGGCTTTCAW AAAAAAATTTTRTTCTTAAAAAAATTCAAAAATAGCTAAGACTTCTCCCA TAAAAATCAAATCAAAAAATGATATTTTCTAATTTTATAAAACAAATGTC TCAAATATATAATTTACCTATATCAAAGAGGGCTAATTTTTAATAAATTA GTCAATTAGTTAAGTTTCTTATATTAAAATACCATATCGGTCAGGTTCTA TTATCAACTCAACTGATTCCCTCACTTCTCTCTCAATCTTTTTTCTTTTG GTAGAAAAAGCGAGTAATTATTTTTTTTTAATATTTAAAACTACATAATA TGTACATATAAAAAACCGTTAATGGATCCCTAATAGTATAAGAGTTATAC TCATAATAAGTTGAGGTGGGGCGAAGGGTGGGGGTGAATAACCCCCTTTT ATGGCTTTTTAATGCTTGATTTTTTCTTCTTGTGGTTGAATGGATCAAAA ACCTCTACATACTGTTGTTGAGTAACTTCACACTATTTATGTATGTTGAA TTCCTAACAGACACTCATAATGAAGCTGGGAGTACAGCCCTACTTCGCAG TACCAGGGGTAATTATTTCTGCTGATAAAGAAAATATTTGGTTTAAAGGT GTAGAAGAGTTTTATTTATTAATTTTATTTGGTGAATTMTTATTTGAAAA AAAAAAAAATAGGAGACTCATTTAAGAGTATCCACCCATGGACAACCATT TCTCTGATAGATAAGGAAATTCTCATTCTTTTATGGGTAGGATTGACRTT AATTATGTATACCTATAAATACATATAAAAGCGGGTATAAATAGGGTATN ATAAAAATGGCCAAGACACTGATGGATGTGCCTTAAAACACAGTGTCTTC GTCTTTTGAAAATAAATAAAATATTTTTTTTCATCATAAAATCAATTTAT ATCGATTTTTTTATATGTACATATATCTAATACGAGTATTTTTTGAGCAC CCCATTTGCTATATACAAAAATAATGATATTGAGTATTATAAAAGAATAT TAATAAGCAAAAAAAGAGTCTCTGCCAAGTTTTAATTACTTATTCAAGAC GACTAAGTACACTTATATGAGGACTAGACCCTCACAACTAGGTAGGTATT GAATTGAGTAGGTTTATTAAATAAAGACAAAATAATTTGTACATAAATTC TTTTGGTAATATTTATGTGGTTGTGATGAGACTGGAATATCAATTGGGGT GATCAAGGAGAGTAAGATGTAATAATTGTTGTTATTGTTGTTCAATGTGA GTGCTGAATGAGTCAGACAAACTTTTAAGGGGTCAAAAGGAAATGGCGTT GATAGCATAATATGTATAAATGAATTTTATGACTTTATTTATAATTAAAT TAGTATTGTATAGTGTTGGATTTGGGTTCGAAAATCCTAGGCCAGTCACA GACCGTTATTATTTCTTTAAGCACAAACTCATTCGCTGCAACTTCGCTAT GGGCTGGTTTTGTTGTAAAATATGGTTTCCATCAGTAAAAAAAATGATAT TTTTATTGAAAATATATTTCTTACTTATTAATAGCACTGGTGTTGGAACA GACCTCCTAGGTAATACCTACGCTCAATTTTCGAATTAAAAAAAAAATTG AGTCGACTGAGCATTTTATTGAGTAGTCTTGATTTTCATCACCGATTAGG TATATACAGACATTGTAGTATAACAACTAATCCACCTGACCAAGGAAATA TATTGAAGTCAGTCTACATCATAAATATATATTTCCTTCTCATGAAACTA CCAGATTACAACAGCTTATTAATTCTTTGAGTTTCATGGTGTTGATATCC CTACGTGGTGTTCTAATCAATCACAATTTCGTTCTTCTAATCTCTTAGGA GACAGAATAGACATATAGCACAATTTTGCTACGAATTGCAGAATCTCTCA GTTACTAAATAATATTAGCTCATTACTGCACATAGAGTAAAAAAAAAATC TCCATTATTAGATGAAAAGTAGATGATAGATAGAGTTGACATTTTTGTAA GTAAGAAACTATCTTCAAAATTATAAAAGGAAATATGGTTGATGCTTATT CTTTTTCTTTTTTGTTCATTATTTAAGAGATCGTCGTGGTGTTCACTTCT CCTCCAGAGTCAGCATTTTTACGTATCTGTAGTGTAAATTGGTTTAAAAA TGCATAATGAAATAAATATATTCGAATTTAAATATAATTTAAATATTTTC TCTGCACTGATGCTATTTGAAATCGAAAAGATTCTCCTCTTCAAAGCAGT AATTCATTTTCTCCCCCGAAATTCGAATAACAGACAGCTAATATTAATAA GTGTCTTAATTCAGTTGTACAATACATATCGCTCGTGCCTTCTCCTTGCA CTCTTTTTTTCTTCTTACAAAAATGTCAAATCCAGTGATACATAAAGTAC ACCCTGAAAGTATTTCATTGAATATAAACCAATAAACAGGCAGTACTGGG CATGCCAGGCTACTCTACTTTTTCTGTTTTTTCCCTTTTCTAATTTATCA ACTCGACTCAAGTCGACACAACACTAATTAGATTGTTAGTTAACAGACCG TCCACATATATGGTACCTACTACGATAAGGAATCTTGAGGAGGTTGTATA ACAACTCGTAAAGTCTAATATGTGAGTAAATAGTGAGGCAATGCCCTTTT TATCCAATTATACCTTTAGGAACAATTCCTCATTCTTGACCCCTAGAATG TCATTAATATTAAAGCTCTGAGTAATGTTTCATTCATGTAAGTTATTTAA ATATAAAATTGGCATAATCTTTTGTAGCGTTTCTGTTTTTGGCTTAATGG AATTAGTGGATCCCCTTATTTGTGTCTTCACTCCTGAATATTTTCCAAAA TATAGATATATGTTCGTCCTTTGTCCCTCGTTTATTTCTACTTCATAATA AGTAAATGTAGGTATTGTTATTTTTCTCCTTCAATCTAATGATCAGTTGT CATTTGTTAGTTTAAATATAAGTAGGGGAGACAGAGGGAAGTTGGCACAC TTTTTATTTATGGTTCTTAACTCAAACTTGGTTTAACATAAACTCGTTAA AATTTAACACAAAATACTTCTTTATATATTTTCTAATGTTTGAATATGTT TAAAGGATATATCCGTCAGTAGTTGTTCCAAAAATTGAATTTAAATCATA ATTGTAAATAATAGTTGCAACTGAATTAAATGGCATTCAAGCTGAGAATT ATTAAGCCAATTCCTTATCCGTTTCACGACAAAAGTTTGAGTTTTAGCTT GATACGGATGCTTTTCTGGCCAGTTTCCCGGTCATAAACAAAAACCTATC ATTTTTGGAGTCACTAAGCCTATTTAAACATTCTATGATATCACCCTTAT AATCTGGAAAAATTGGTGTCTATTGGTAAAACACCGATAGGTTTCGAAAT TTCCAGACACGGCTAATATCAAATATTTGATGTAGTTGCTCTCTGGCCAT CCAAAATGATATTTTTTACGTAGTGAATACATTTGCAACAGTTGGAAAAT CAAGTTAGGGTAATCAATTAAGAAAATTAGATATAGTAATAATTTTAGAT GAATAAATAACTCGACTTTTCATGATGTTGACTTATTAAATTTTGATGTA TTTTGTCAGATTCAAATGTAATAAGATACATCGAATAAATCATCCGTGAT TAGTATTAAAGATAAGAGACATTTGATCTACAAAACTCTTCAGGACCCTG CTCTGCCTAATATACATGTTTGATGTGAAGTGTAATTATATTAAAAAACT AGCAGAGGATTCCTACCTTGCAGAATTGAATAGAAGGTAATATAAACAAA GAATGAAGGTAGACAAAAAGAAGACAGAGAGATGTAGAAACAGAGACGCT TAAGGAGGTTTTATTGCGGTCAAAAATCGTCTCTTAATCACCTCAGAGGC CATTTAAAACATATCATGAAACATACTTGTTATACTAGCTAAATTTTAGA CTGATCTGCTTATGTGTGATAACACATAAACAAACAAATTAACTAACTTA AAAATATCTAAGGTGTGTTCAAAAAGTAAGATTACTTTTCATAAAATTTG CGGGCAATGCAAATTTGGTATGCTCGATTTTTATGTACATAAGTACTCTC ACCAAGAACATATCTTTACTGTTTCAGCTATATACTTTGTCTAGTTTGTT TGTGAGAGGCATACCGGTTAGAAGTGTTTTGGGTGTTCATCGACTTTTTT TATCTCTATTGTGCTCTTCCTTTTGAACAAATTGGTCTTTGGCTTCGACA TCATAAACATATATGCATGTTTCGTCACCAGTTATAACCCTTTTGAGCAA ATCAGGATCATCATTGTTTTATAAGATTTAGAATAATAAACAATCAAAGG AAAATTGGAACAATTGTTGCAACTCTCCCCTTTGTCCAGATCGTTACTAT TTCTCAAAAGTATCCTCAATGAACTTTGCTATGACTATATCAACTGATAC AAAATAGTCAATAGTTCAAATGTTATAATTTTGCAACAAAAACAAATCAA AACTGACAGCCTTATTTCTAGTAAAGGAACGATTAATCGGAATCGGAGAA TTGTATGTTTTATCTGGAATTGGGATCGGCATGAGGGAAAATAATGTTTA ATTTGAGATTCAGATTCTTATTTATTAGTAGTATTTAACTATTTATTATT TTTCTAGGCTATGAAAAGAAAAAATCAATCCTCCCAAATTAAGGAATTTT TGCTTTTTATAAGAAAATTTAACATTTCGAAAAGAAAAAAAATCCAAATT TTTTTTTAATATTTGAAACCAAAATAATTTTTTCAATTTTTTTTCCAAAA AATTAAACTTCCTGTAAAGAACTATATATTTTTTCAATTTTTTTCCCAAA AATTGTAATATTTGAAAAAAATTTATTTCAAAAATTTAATTTTTGAATTT TTGTTTTTTGAGATCAGCAATGAATTTTTTGAAACTTTTTTACAAATAAA AATATTTATTTGTTAACAGTTGTGAATTTTGAAAATTTTTCCCGAAAAGT AAAGTTTTTTAAATATAGCCATCGGGTTTTGAAATATTTTTCCGAAAATT CTGTCGATTTTTGAAGAAATTGCATATTTGAAATTTAATTGTTCAAATTA TTTTCCAAAAAATTACAATTTTAGGATATTTTTAGGAAATAATTACAAAA ACCAAACACTTACCCCTAAAATGTAATCTCGTGGACGCCGCGGATATCAA AATTGTATTATTACCTACTATTTAAAATATTTTAAGAATAGGAATCGGCA TTGATAATTTTTACTAGAATCGGATGGGGATTTTTTTTTTTTTTGAATCA TTCCATCACTACATATTAAAGTAATATGTAATTTATTATCCTATTTAAGT CATATGTATATATTTTTTGCTTTTTTGATATTCGTGAAAAGTTAACTGTT CCGGGCTCCCCTACAATGTATTTGATGAGAAGTAGAAATCAAGTGAGTGT GGGCAAAGCTTGTACATACCTAGTGTCCTAGCTACATGTACATTGTACAT ACATATGTATAAAATATGTATCTACATACTAAATGTTTGTTTGACTATGG GCAAAGCAAACACAAAATAGAATGTATCTACGTAGTCCCTTGAAAAAAAC AAAATACATATATTTATTTTTATTTTTTTGAAAATATTTATTTTGACGAT TTAAAAAATAGTTTTATATTTTGTGTACATATGTAAGTATGTATGTATAT TAAAGAAGAAATATTTCATCGCGTACATGGCCTATATGGGCTTTAATAGT TCATTGAAAGAATATTTTAATACTAAGTTTAATCCCTTAATGAGTTTTAA ATATTTGCATACGTCTTTGGTTATTTTTGTTTGATATAGTTGGCATGTTA TGTAGTTCATTTTTTGAACTGAGTGTGGATTATATGGTTGTATCAGTTCT AAATATATAACTTTAAATCGCCCATATTGAATGTCGGGTACATTCTAGTC TTGTCTCTTTTATTGTCCTTGAAAAATGTACAAAATCTTAAAGTGCCCTC TAAAGACTTTTTAGTAATTAATATTTTAGTAAAGTACCGCAGCTTACCAA TTAAACATGTTCAATTAAACTTTAAGTGACTTTCAACCACGATAATAGTA TGTCCTTATTCTTAATTTAGGCCAAATTGAGGTATTTATTAACCTTAAAA ATATTAGATCCTAGATGGTCAAGATTGTTGGATGATGACGTCACTGGTGC ATATATATGATGTCATCATTTATCAAAGGTTGTTTGAATGGCGACTTTTT CGTCCGATTTGGACGCGTCCTAAATACTAAGGACAAAGAAATCATAATTT TTGAGACCGTTACATCACTACTGGATTATATTTATATTCTTCAAGTGATA ATATTCTATTTTTGCAAATATAATATTTTGATTAGTCGAATTACTGCAAA TTATACTTAAAGGCTGCAGAAATACCTGTCATAACTGAACAAAATAATAT GATAGATTATATGGTTAAAAGGTCAAATTGGGATCAAATAATATAAAATC AATCAATCAAATAAATATTTTGAGGCATAGTAACACTATTGAGTATTTGA TCTCATCCCCAAAATTTGAATATAATTACCTTAATGTACCAAAATATGTT GATAAGGTATTGACCTCTATGCATTAATTAAGTCATAAAAAATACATAAG GATTTTTTTAGATGAACAAACTCAAATGATTTTTTGTGAAAAAGTTAAAA GATGAAATACTAAAATGTGTAATTTGGTGTAGAAGATGCATTCTTCAAAT ATGTGTCCTAATGATTTTGTACATGTTAAATTTTATCCTTTGCTACAAAT GTTTACATTGAATAGTCATTCAGGCTTCTGTTTGTTTAAAATGCAACTTT AGGCCCAAAATATTGACCCTAAATCCTAATAGTACTCTAAAAATTATTCA TTTTCACATCTTTAGTGTAAATAAATATCCCTCATAACTTCATCCAAGAG ATATTCCCCTAGTTAATATGTATTACACAGTGCCCAAATGATTATGGTAT AGATTGATGAAGGGGTGGTAGTATTAGCAGGAGTCGGTTTTGTATATAAA TATTTGAATATTTTTCATTAAACGACGACTAAGTGGATTTTCACTTTTTT CTCTCTTTCCATCTTTTCTTTACCAAACGACTTCCCCTAATAACAATTAT GATTATATCATAGAATGATTTTTTATTCTTCTTCATTATTGTACAACCAT TTTTATGCTTCTTATTATTTTTCTTCTTTTATGACAGAACCCCAGCAGGA AACAATTCAAATTCATCTCTCATATGAGAGATATTTGTTTAACTCTTTCT ATTTGAAAAGTACAAAATAAAGGTGCCCTGCCTTTATTGAGGGGATCTTG CTTCCTCTTTGGGTGTGTGTGAGTGACTTTTTCCAGATGGTTTAAAACTG GTTCATCTCTTTGACCCATCTACATAGTATTGGTTTTCGGTGTGGAATTC CTTGAAAGCTCTGAGACACTATTTTAACTAATTTGGTCCAACTTCTATAT GGATCAATATCATAGTATAATTCGGTCTACAAAAATAAGTCTTGATCCGA TAACAAAAATAAAAGAAAAAAATTTATTTAAGAGAAAATATTATTTGACA TTTTTTTATATCAAAATTTTGTTCAATGATTGAAATATCGATCCTCGGCT TCAACTTTGGTCTATAATGTAAATTTGTGTCCACGGTTTCAAATTTAAAA ATATTGGTACAAATCAATATAGAAAAATCACAGTTCAAAAATCTTTGTTA TCAGACAGAGTTTTTACACAAATAACTGGAGTTTAAATATGGAGTATGGC TCTTGGCTGATTGAAAACAGCTTTAGATTAATTGATATTCACCCTAATTT GTTGATTATTTCAGATATTTTATTTTTCTTAAATACAAGATACAATTTCG TCCCCTGCCCCCTCCCAAATAATGAATTAACAGTTTTATTTTATTTTTGA ACCCCTGAAAAAGCAAATGTGTCCAATTCAACCAATCTGAAAGTTATTTC ATCCGTAAATAATAATATTTTGACTCTGATAAACAATATAATTTAGGATA AGAGTATCCGACATCAACTTGAATTGGTTTTTATGCTCAAAACTTATAAA ATTTACAACAAATCCTCTTTATGGCTAAACGTGCCATAAGTAAAATATGA CTGAATCCACAATGTAATTATTATCTGAAGAAACGTTATAGGATCTAATT TTATTATTTTCTTGGCCACTGTGATGAAAAAAAGGTTTGATATGCCAAAT GGCCAAAAAACCAGCTGATAAATTTATAATTAGTTTTTAATGCTCAAATA ATCTGTATGGGTCCACCAAAATCCATATCCATCGATTCCATATATGAAAG AACCTCTTTTATCCCTAATAGTTTTTAAAATATAAGGCCTATATAATTTT TTTTAAATTAAATTATCCACAATCAATTATGTATGCGTGTTGAAAAGTGA ATGGCTTCCCACTATCTCCCTCTTTCAATCCTTTGATGATGTTTGTTTGA TTATACTAACGAAAAATAAACGTTTAAGTAATTTTTGGTACATTAAGATT ATATTCTGTATTTTATTTTTTCCTTGTAAAAAAGAACTTGAATACTGATT TACATATGCACCATAAATAAAGCAGTACACCAAGTATTCCAAAATATCGC CTCTACAGATATATCAGGCTTATTATTATATTATATATTATTCTTTATAT CCCCTGAAGAAAAAATAAATTTTTAAATAGAAATAAAAGATCCACATTTA ATATGATTCCTATATGTTTTAGAAGATGAAGTTGTAAGTAAATATTAATT TGAAAAAAGTTGTGTACAATATCATTTTTTATCGATTTTCCTTACTAAAT CATTTGTAGTCTAGTTTTTAAATCTTTGAAAAATTACTTTAAATGAAATT TCTGATTTAAAAAAATTAATTCAGAATACCGTTTTTTTCATGTTTTTCTG TTCTATTAATTTTTCCATTAACCAATACGTTTGCAGTTCCGGAAGGTCAT AAATAAGACACTTGCATTTCTCCCCGATTTTATGATCTATTTAACTGAAA TTTACAAGTAAATATCATCATGGTACAGGGATATCAAACATCACAACTAA CAACTGATTATTTTTCTAGTAATATATCTTATTATCATATATAGGAGTTA ATAAATAAAAAATCGTATTTGAATTACATAGAGGTCTTCTAACCCATATA TTTGACCTCTTATTATGTTCTCTCTCTCTCTGCACTTTGTGTCTTGTTTT TTTTTAAAGATCTTTAAAAATAAATTCAAATGGTGATTATCTTAACCATG AATTTAAATTTAAAAGCTTCAGTGATGAATTTAAAAAGCCATGGGTATTC TTTGATTTCTTGGAAGTAATCAACGGAAACTCATAGCACTTTATTTCTCC CTATAAAAATGTACAATTTTTCGAAGAAGAAATCAAGAAGTAAAGGGGGT TAAGATGATTTCCTTTCTACAAAAGAAATGACTGTGCCAATGGCACAATT GTAGTCTAATTCCAGGAGCACCCTGAAGAGAGGGAATCCATATAATCAAG ATGTAAAGTCGTTAGCCCTCAACATCCATAATTCTAAGAATACCGTAAAT GTTTCATTGGAATTTGTTTATATCACCTGATACATACCATTATGAGTGAG TCACGCAAACGATCTTTTTGAAATAAAAGAAATAGAAATATAAAAACGAA TCCTGAAATCCATTAGTTCTCATTGCTATCTACGAATATATATTGAAGAA TATATGTTATATACAGTGTGCATGCGGAAAAATGCCCTGCTAAATTGTAT ACTATTTTTGTCAGCAAAAATAGTAAAATGCATTAGGTGAAACAAGAAAT AAACTTACGTTTTTCCTCCTAAAATTACAATAACGTCAGTTTACTATTCT TTACTTATTTTTTTTTAATTAAAGATAAAATCAAAATAAACTGAATATTA TAAGCAATAATTTCGTGTAGCTGGTTAATGAAATTTTTGACTGATGAAAT TTTGTGTTACAAGAATACCCTTGTCTATTTTTAAGAAACCAAAATTGCTA GAATAAGGAAAAGTCCGTATTTCTCCTTTTTCCTTGTATAAACATCATTT CTAGTGTCCATCCCACTTATATTTTAAATTCTAAATTAAAAAAAAAAGAA TCTTTGTTTTCGAAAGTTTGATCAAAATGTTAGGAATGAAAATTAGTGAG TAGCAAAGAAAAAGACAAATAAACAAATCTTTTGGGCGTGCAATTGCTCA AAATGGCTTTGCCATAATTAATTGGAGAAGTGAATGTCCCCTGCTTTGTT CAGTGACTATAACATACCAAAAATGCTAGATTAGAAACTGAGAGAGTCTG CGAAAAGGCTATCATTACCACCAAAGAGTAGATCATATATACTGAGAATG TTGTCCTTAAGACTTTTACCATGTTAATACCCATAGTCCAGGTTGTCATC AAGTCCAGTCGAGGACATATAACATAAAATGTATTATCTTTTAACATTTG TTGTGGTACGCCTATTATATAAAAATTTATGTAATCAAACTGTACATTTT GTCATACGCACCCTGTAGGAAAAACTCTGATAAAAGAAGACATTTAATTT CCTCTTATCACTCTCATTATCACTATTACATTTTTATTTAACGAAAATAA GTATTTGACATAATAAAATTAGAATTTCAAATGAAATTGCATATTATTAT AAAAAATACCCAATCGACGGTGTTGAGGATTTATAATCCAAGGCAATTCC ATTATTACATACCATACATATGTTGTTCATTTTCATAACCATAAAGGTTC CTTTCACCCATTTTTATATGTACTTACAAAATGGGTATATAGGTATATGT AAAGATTTCTAATAAGGATATGGTAATAAGAAGATTACAACAACTTAATA AGTGGAGTCGTCCTCGTAGGTGGATAGTTATTTTTTATGTATTTTTTGGG GGTTTAGCTCTTCCAAATTTATTGGAGGCCCCTACAAGGGTTTATATTGT TTTTTATATATATTATATGTTATTATTATTACCCATCTGTATTTACCAAA TATGTACCTATGAAAATTAATTTTAATGCTTCATAAAAGCGGATGCCTAA TTCATCAATGTTAGAGCTTTAAAAAAGAGTAGTCTTCCTTTATTACATAG GGCACTATAAAAAAAAGGTATACAAATATACTGTAAGGACGACTGGAGAG TGAAACATGAGACGGTTACGTCAGTCCTTATTTAGAACTGCAGACTCTAG TCCCGTCCMGTTCAGTCCGGCCCAGTTCAGTTCTGCATATCAGAACTGCA ACTTAAAAAGTTTGGTCCTTGATGACGTCTCTCCACTTTATTTCTCCTTA AATTTAATCAGTTCCAAGGATTTATAGGACCGGTCCCAGGGGCACCAGCA GGATTATATATTATTATTCATTTTTTTTTGAAAAAAAATCCAAAAATTAA TATCTTCTGGGGAAAATTTGAAAATTAAATTTTTTCCCAAACTATTTCAA AAGTCATATCTATCTTCAAATAGAAAATTTAAGAAATCAAATAAAATTTC AAATATTAAATTAAATAGTAAAAGCAAGAATTCTTTAATTTGGAGGGCGG CTACAGTCCTCCAGACCATGCCCCTGGGTCTAAATACTGGACTGGACAGA ATAAATAAGGACTGTCACAACATTACTATTTTCTCGGACGGAAAAAATGA TTCAGCCATAAAATTGTAATTACACTTGTATAAAATTGTACGCATTATTA TGAAAAAAAAGTACATTTATTTTTGTCAACTTTTGGTTTGAGATATTAAT TGGATATAAACTTTTTTGAGGTGTCAAATGCTTTTGTAATATTTTGTGCT AATGCAGAACGTTTGTCTTAGTTTCATATTTAATTGATATCAATGATTGA TTAGTTTGCTATCAGTTGATATGATTTTATCAAAATTCAAAGCTAATTCA TTTAGCAAGGAGATATTTCAACGCTCATTACAAATGGCATAGTTGCAACA AGTGCTTCAACTTTCCTTGCATTGTTTTTTCTTCTCCATTATTCAATTTA ATTACACTTTGCAAGTATCTCTGTAAGCCAGAGCTGGACTTTGAATAGAT TTTCCGATCTATAATGTTGATTTCTGATCTGTAATATAAATCTTGTTTTT GTGCAATCTACTATAATACATTGCTAATTAAAAATTATTGAACATCCAAA AAATTATTGTTGTTTACTCTTCAAAAAATATTATTTAGTATTTTTTTTTT TCAGTTTNTACTCAAATTTGATATACATTAACATTTGCAAATGTCCATGT CGAATGAGGACAGTTGCAACATTTGACAAGTGGCATTCAAATGCAGTTTG TGGGAAAACTATTGACTAAAATAATCTATAAAAATACTCAAACAATAGCA GATATAAGTGAGTATGTTGTATTAATATTTGACAATCCTGAGTCAAACAA ACTCGGAGTAAGTGAGCCAAAGAATGCAAAAGTGTTGCAACTGTTCCCGG CCTTCCCTATCTACTATTTACATAAAATATGTATCTTATATTATTAAAAA GTAGAGAAGATAGATAGAAAGAGAAATATGTAGATACAAAGTAGGTATAC ATATATTCATGAATAAATTAAAGATAAAATATAGGTCGGGTCAAAAATAA GAATCTTAATGGCTTTATTAAAGTATATTTATAAAGAAGAAAAATATAAT GATTAAAACGCCATCTTGCGACCAGAGTTTCCCTTTCAACAAAATAAAAA AATATGAAGAAGATGAATAAATTATGAATATTAAAATTATATTGTTTTTA CCTTTTAAAAATAAAAAAACCCACCCTGTTTTTTTTTTCTTTAAAATAAA AAACAAAATGGACGTATCTTTCAATTAAAATATTAAAGTATCTACATATA TACCTACACTTATGACCTCTAATACCCTCAAGGAACACTTTTCTTCTTCA TCATAAATAACTCATTTCTTTAAATAATTACCAATCTTCATTAATTCTAT CAACCCATAGATAAATAAATACGTAGAGGGCACAAGATGATGGAATAATT GAGCAAAGACTTTAGCTGATACAAAGGATATAATTCGACGTCACTTTTTT TAACTTGATTATATTAACTTGTGTTGGAACTCAAAAACTAAAAAGATTGT AGTTCTATCACTACGATCTTAATAAAATGTGTTGGTCCTAGGACGGTTGA ATACTAAAAAATATTTGACTGATAAAAAAAATTATGAAGGGGGAAAAAGA CCGATTGAATACTAACATTGACATATTAATAATGTTTTTGATATAATAAT TGGAAATAAAGCATTTTGACTGACGCCATAAATTGCATAATCATTGATGG AGACACCCTCTTGGAAGCTCAAAGGTTATATTTTACTAGATAAAATGGAT AAATTTTAATCTAACATCTTGATTAAGCTCCATCAAGAAGCTTTCAGAAT AAAAAAGACTTAACATCTGCATTGGATAATTTATTATGTCAATGATTAAC CCATATATTTCAATTGAATCAAAAGTATTATGTACTGAAAATCCCTAAAA AGGCTAAATTTGTACCATTTTTTTTTTTGGATCGAAGAAGGATAAGAAAA GTTTGAACGATAGATAAAAATATCTTACTGTGTTCATTTTAATAGTTAAT TTTTATCTACATTATGGAAATAAGATCGTATAAGGATGTAATAACGTATT TTTAATACATTTTAAATTTAAGGTCATTTATAAAAAAAAACTACGCAATA GAGGATATATTTTTGATAGACAGTAAGTTTAAGTGTTTTTCCTTGTTTGT CCCAATACACAGGGATAGTTTACGATTTATTACTCCCTGAAACATTAGTT GCAGAACGACTACGGCTTTCTCCGCCAGGATGTTATTAATTAGAACAATT AATAGAATTATGGATAATATGTAAACTTTCTAAGAATTTAATTTGATTTA AATAAATCAACATTCAGAACACGAATAATAACTACACAGAGTGCATAAAT CATCAGCTGCCAACTCCAAAAAACATATTCTCGAATCCATACCTATATTA AACACATGAATATTACTTTATCATAATGATAATATAAGGTAGACATTAGA GGCTTTCCTTTGAAATGGTCTGTAGGATAGTTAAACATCTTTGAAACACA GAGCCTTTTTGCAGTCTTTAAATGTTATGAGTATTTTTTTATTATATTAT ATTTCTTTCACAAGYATTGGAAAATAAGTCGCAATAAAAAAATCCAARGA ATAAATTATTCACTAAAAAATTCATGAACTACTAGGTTTTGTGACTAGGA ATGTCCAAAGGATTTTTTAYRGTTGAATWTTTMMSSGAGCCACAAAGATT CTTTTTGTAGGCACAATACAAAAATGAATTTTATAAAGCTTTAAGTATCT CAGGTTATTTTTGTCGGGGTACCCATCANNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNCGAATATTTGGTGTTTCCCTCTTTTATCAAAT ATAATGTTCTTCTTTTTTGTTCAACAACTATCACTTCAATTAAAGTTACA TTTTGCTATAATACTTCTTTTCATCTTTGATAAAACAGAAAAACCGCAAA TCATTAAGCACATCTTCAGATGTGCTTAATGATTTGCATTTTTAAAGTAT AAAAAACGAGGTATTTTATAGTTGTTAATGATAAAACCGATATAAAAACC GCAATTTTTGATTCATAGCATCATATTGGGGATGACACATTAAAGATGGC CGTTTTTGACTCAAATAACCCTTTTTTCAAATTAAAGTGGGTTTGACGAA GCCAAAACTAATTAAAGGGGCCTCAAACTTAATCAGGGTCAGTTTTTTTT ATTGTACCCAGCAGTAAATATCTTTCCACAGCTGGACAAAAATATTAACT CCCAGGACTGTATAATGTACAATGTACATATTTTATCCATTTTAATACAC CCTTGGTGCAAATGATACTTGAAGACTACGAATCATACATTTTTGTACAA CTATTATTTAATAAAGAGGTTGAAATAACATAGCCTGTTTATTATGGCTT TGCTCACAAGCATGTAGGAGTTTAAAATGGGCATGAAGTTACTAAAAGTA TCATCAAATTGATACCTTGAATTGGATCCTCTCCAATAAAGGAAATGTTT ATTGCTTATTTAAGCATGAAAATTGAATTGTTTCATTGTATTTTGATACT TATTAACTGGAGTTAGGACCATACATTTATGTACATATTATAGGTATCTA CATCAAAATTGACATAAATAGTGTCCATTTGATTCGAAATGAGTGCCCAT TTATGTATTGGAATGAGTGTAGGTTTCAATTAGAGGAACTGTTTCTGTAA ATAATAATAATTATGCCAAAGAGTTTCTTACGAGAAAACATGGATACACT AGACTCAATATTCATTTTGAGTGACTTCATTTTTTTGTCAGCCGTCCATG TTTCTACTTCACAGTATTTTAAACCTTGATCCTTTATACTCAAAAAGTTT GCTGCTGTTAATTATCTGCTATCAGCTATGTACAGAGTGGGAATACAAAA CTTGTAGTTGCATGACTAAATTACTAAAGACGTTATTTTTATGGACTTCA AAAAAGACAACTCAAAACCAATTTGGGATATGTTTAAATATTATATACTA TACTTTGTATGTCCTCCTTCACATGCAATAACAGCCTCGACACGCCGACG AATAGATTGGCTGSTCTTGAGGAAAAAATGTCCATAGCGTACCACACTGT CTCGATGGCAGCTCAGAGCGAATCCAAATTTGGATGAGAGTCGTAGCATA CATTATTTTCCAAAACACCCCAAACTACAAAAGTACAGAAGATTGAGTGG AGGGGTCATATGGAGGCAGGCCAAAAGTTAGCTATACTATTGCTGCAACA GTTTTGGATCTTCTTAGCTGTATGGGGTTGTAATTGACTTTATGATGTTG TAGGGAGTGAAACTGGAACTGCCAACAGTTCCTAGCCTTTTCGGCACTGA CACTTGCACGGAAGAGATTACAAACCCATTGACTTTGTTGTTGTTGCTCC GACATGTTTACACTTAGAGACATGGGATAAAATAAAATTTGTTTACTTAT AGCTGTCGTTGTTTGTGTAATGAAATCTCGTTATTAAATGCTACAGTAAT TTTTGTGTCCCCAATCTGTATAAATGAAAAATAATTCTATAATTGGCTGA CTAGATGCATACAAGGAATCATTTAAAATTACTACTCTGGTAAAAAAATG TAATGAAATTGGGAGTTCTAAAATAACGATTTCTTTTTTATCAAAGTAGT ATTTACTAAAACTATTTATTTAAATATTCGACTAACGGGTCCATAGGCGA TAATTAAATATTTACCAAAATATATGGTATTGTAGCATAAGTGATGGTGT TTAACATGGATCCTATTGATATTTGATAGTGAAAATACGTCACTCACATG ACAGGATTTTCTTCCATGAATGAAAAAATCATAATGATAAATAAAATGTA AATATTCATAGATAAATACATAAATATATTAGACATTTTAATAGAGCTTC ATACTGTATAAATTATACGTCCATTGATACTGATGATGCCTATACACTAC AAACATAGAGGTATATATAAGGAAAAAATAAGAAGAATTTGCTACTGGGT GACCTTCTTTATGTGGGTACATTAGGTCGGACTGTTTCTCAACTTTTTTC GAATTTCGATTACAGGAAATGTGGAAAAGTTGTAGTCATTTAGAAGGAAA ATTCTCTATACAAAATGTCCTTGTTCTACATTAATATCATAGCACACATC GCGATCGATGTTTGAAATAGACAAAGAGTATTTATTTAGTCAATAAAGAT TAAAATAAAGCATTATTTGAGTTCTGCTTAAAACAATTTTGACAGACAAT TGTCTAACCTAAAGGTTTGCAGGATCATATATATTTTTTTTTGTGTCTTG GTTTTTGGAATTATGAAAAAAATTAAACATTTTTTGAAAAAAAACTCAAA ACTCCACAGGTGTTCACATAAAATAAATTTATTGGAAAAAAGTTTCAAAA ATAAATTACAAATTTTTAATTATTTGGAAAAAAATATCAAAAAATCTACA ACTATTCAAAAAAATATCAAAAAATCTACAACTATTCAAAATAAATCAAA TTTCAAAAAATAAATTTCATATATTAAATTTTTGAAAAAAAGTTATAATA TGAAATTTTTTCAAAAAAAATCCAAAATCCAAAACCGGACAGTTAAAATT AAATTTTTCGGAAAAAAATATTACAAATTTAAATTTCGAATATTCCATTT TCTAGTAAATAGCCAAAATTCCATGTGTTGTTTTTTTTTTTGGGGGGGGG GGGGGGTGAGACCTTTGAAGAWACTCTAAAGTAAGAGGAGATTGAAATAA TAATAAAACAAAACATCAGATATATCAATTACGTTCTCACATTCACACAT ACATCGATATATATTTTTAGTAGCAAAATTATCCAAATCCACTGAACTCG TGATATATGAAAATATTCGTATGGTAACATTCAGTGGCGCCAGAACCATA GGGACCCCCACCCCTTTTTTCAACATTGATATCCCAATCATCTTGGAAAA ATAAACCTTCCTAATATATTTGGATACATATTTGTAAACAGCAAATATTA TTTTATTTAAAAAGCACAATATTATATAATTGTTACAATGGATAGCAAAA AACTTATATCTATATATTATATGTTTGTATGGATTTGTTATGAATGGCAT GGCATACTTTCCCTCTCAATATCCACATAGTACCAATGTTGGTGATTCTT CTTACATTCATATATAAATAGTTATATGAATAGGTCCTTAGATCTTTACA ATAGAACGTGCTTCACTTATAGAAACATAATATGTTCAACGTACATACAA CAATGTTGAAAATACAGCAATTTTAGGGAGACTATTTTGTTTACACCGTT TACAAATGAGTACTTTGTGATTCGCATCGACCATATTTGTTATTAGTGTG ACTAACCGTATCTTTTTACATTCTTTCCAAGAATTTGTTTATGTATAAAT GTCCGGATTTATATAGGAGAAATGGTGCATGTTGAAAATTGACTTAAATC GAAAATACATTCTAAATTTACATAGTTAAAAAAAAAAAAAAATTCAACAA GTAAATAAAATCCAAAATATCTTGAGTAAAAAAAATTTCTGAACATATAA GAATCAATTATACTACATTCAAAATATCCCTCCTATCTTAAATATTATCC TCTTATTTTTTACTTTGTATTCAAATTTCTGGGATGTGTTGAGATATTCA AAAATGTTTACTATAGTTTCAATCCTTTTTTTGATGTATGATACTTAATT TTACCCCCCAATATGGCCTTTCAAAAAATATATCAAATTATAATCATTTA AAATTGATATCATGAATTTTTTGTATTACTTTTTGGTACCTTCAAAATAT TAACATAATAGGTTGTTGATTGAAATTGACGGTAAATTCATTCAAGAACA GAAATGTAATCAAGAATATATTTTGATAAAAAAATTATAGCTTGCTTCTG TGTTGATAGGTTGCATATGTATGATTTACAAACTATCGAGCTTAATATCA ATAAATGTCATATGTATACATTGTTTTAAATCCAATAAATCACCCAATAT TGTTTTTGCCTATTAATATTTTCGTCGAAAAATAATAACTTTGAATCTTC AACAACATAGCATTTAATATGTAATTACATCCTTCTGCAGTAGGCTACTT TATATTGTAATCTATATGTATATTTTTTTCTATAACACATACAGCTCTCG CCATGTGGGCCCTCACAAAAGCCGTCTGACTTACAACCTATAAAACAATA AAGATTTTGATGTCTAAATTTGTTTTTCTCTTGATATTAAATAATAAGAG TAGTTTCTGGGCTCATGCAGGTATCTTGGATTTATGTACATTAGTTTGTG TTGTTAGTAATATTATTTTACATCATGCGGTTGTTTATGAATGTAAAAGC TCAGCTTGTTGGTAGTACGATCCCATCAAAAAATAAAATGTTTTTGCAAT AAAATTAAAAAGTGTTTATTGTTTAATGAGAAGTATATTCTTTGTAAATT AAATATACAGTGTTATATACTTATACACTTAGATACATACATACTATTTT ATACACGTACTTAAAACAACGAGTAGAGTAAATAGTCCAATTAATGATAT ATATATTCTATTTGACCACCTTCAGTAGCAATTAGTTTTGATACTCAGTC CAAGAACGATTTTTTTTCCGTTATGATCTTTAGGCATTAGTTATAGACAT CTCTTGATTGAAATTATAATCATTGACTAAAATATGAACTGTGTACTCAA GTTCTAATCGTGTACCGAAATTATCATTTCTAAAAAAGTCATTTTTTAAA TAAAAATTCTTTTTTTAAAAACGATGTGGAACCGAATGAACACCACATTA TATAAAATCGTAATGGTAGGATTCGCAGACCGAACCCCTATAGTTGCAGC AATAATTCACTCAATACCAGGATAAAAAAGACATACATGTATTTATTTAC CAGTTAAGAACAACTTTTCAAAATACAGTTCATTTTATCGCTGAAGAGAA TGAATCTTTTCTCCATTAAACTTCATTTGTTGAATAATGTGTAACCTATG GGGACTTTCTTCTTTAAGTATAAATGAATAGTTGAGGAGACAGTTGAATG GTTCTAATTGATTTATTTTTGGTCAAAATACTTTTAAGAGTTTAGTATTT CCTATTTTTGTTACACCTTGGAAAGTTGACTTTCCTAACATCCTTTTAAT CATTTTTCAGCGATTTTAGGGATGATAAACTGTGCTTTTCAAGTAATCAA GCAAGCTATTGATTGCACTAGGACAGTAACCCACGTGAGCTAATTGAGTC ATTGTATACTTTTTCATCTTTAAAAGGATCTTTAATTTAAACATCTAACA CTTCCCGAAAAAAAGAAAGAAATACTTGCTTTTGATTTAAATTTAAATAA GGTTTAAAATACTCCGGAATTATCTAAATGACCTTGTATAAACCTTAACA TATGATATAGGTATAGGCCATGTGGAGTATTTTAAGATGTGGCTCAGTCC TGTTGCTTCACCTGTTTTTTGATTAATTTCGTCATCGTTACGTTCCACTT ATTTTATCAAGAAGGTATTTTCTTTTTGTCGGAAAATAAAACTGTTTTGT TTCGTCGTTGACAAATATATTTTGTCAAGTATTATCAGACCAAAGCAACA CTGATGTTGTAACTCTTCTACGTACATGTAACTCTATAGCTCAACATGAA CTGATCAGTGAAATTTGGGAAGAAATAATATTAAGCCAAGGACGGTGATA GTATTTTTCTTTTGGGTAGGAGTAAATATATTTTTTTTTTTCAAATTTTC TCACTGAAATGTTTAATTGTTTTTGTTTTTTTTTAAATAAGATTTGGATT TTCAATTTTTTCTAATGCTAATTGACTTTCTGAGTAATTTGCTTCGTTTT CGATCAAATGAGTTTTTTGTTTTGTTTTTTGGAAAAAATAATGTTTGAGT TAATTTTTTTGATTACTTTTTGTCCCCTTTTTCAATGGATAATTTTTTCC AGAAAAAAGTACTTTATCTTTTTCTTAAATGCCCTTATTCTGAAAAAAAT GTACATGTATTTTTTTTCAAATAATCTCCATTTTATTTTTTTAAGTCTTT ACCTAAATAATCAAAAAATTCTGTGTGACATCTTATTTTTCCAGACTAAA TAATCCATTGGTGTGACTCTCRCCCCCCCCCCCCCCCCCATGCAACACCT ACATTGACCTACATCTTTAAAATACTTTATAATATACAAAGGATATGCAA TTTTTTTGAAAGTCGATTGCTTCATATTCATTGTACATTTGATCAAGTGT TGAAAGCTCTTGACAAATTTGTGTGTATTTTATGATTTTTCGGGGAGAAA CTTTAAGGAGCGGTGAAGCCTTTGGATAATAGACAGRAATAATACATAAA CCCTTATCGTAAGAAGAATAAAGTGATCCTTGATTTGTCAAATAAGGTGA ATATATTGAAGCTATCTCGTTTTAGRKCCCRACCSGTKCCKTATAAMTAT AKAATANNNNNNNNNGAACTCAAAGGGTCCGGAAATACCTTCAGATAACT GGAACACTAATAACTTTATTTTAAGTAACTAAATGGCTCCTTTACGCACT TCCACCCTCTTTAGATAGGATGCATAAAATATCTACACAGCTTCTATGGC TGMATATTTCTTTWAATGTGTTTTTCCATTCTACAAAACGGAATTTAAAA GCCCATAAATAACGCATTTAATTTAATCCTGCCCAAATACGATTTAATAT TTATAAATAAAAATATTTCACTCAATGAATGGTATTATAAATAAAAAGGC TATGAATTGTGAGACCCTGATATCGAATGATATAGTTGACCTACTAGTAT GCGTGTTCCCTAAAAAATAACAGTGAATTCAAAGCCAATTAATTTTGGTT CAAAAAATATTTATTTTTGTTGATTAGCTTAATTTACAGTTTGACATATG TCATGATAAGTATAATCTGTGTATCATGATCTATTTGTTATTCAGCTATT TTCCCGATAGGTATATTATAAAATAGATGAAAAGCTATTCAAGGCTTTGA TTCATTTCATTGCAATAGTTCTACTCTATGTGTTGGGTCTATCCTAAAAA TAGTCTATGGGGGCATCCTATTTTTCAGAGCTTTTTTATAGACATAATAA GATTGGTACAAAAAAATGAATTGGTCTTCTTTAGGGAAAAAAAACAATGT SCGACGACATTTACTTTGATGTAGAAAAATGGATTTTTAATNCATGACAA TTTGAAGAACATTTTGGAGAAGAGTAACTTAGCTGTCATGCTGTTTGATT AGATTAGCTGCGAGCAAATGAACATAAATCCCACTCTGCTTCTTTGCAAG GACTTAGGCAGGAATGAACCCCATGTCAATTCACAATTCTACTATGAATA TCCGTATTTCACGCGCAGGTACTTTATGCATAGACATTATCTATTTAGGA AGTAAATAATAATGATAACAATATTGGAAAATAAAAACAACGTTCTGGTT GCAACTACAAAAGTATCACGCTTGTCTGAACTCATATTTTTTACAACTCC AAGAAAATAATAAAAATTGTCTATTATTTTTCGAAAGAGAGGTTTGGTTT GATTATTTAATCATATCACATCTAATCAGTTTGATTTGTATTCTTTTTAA ACATGGGATTATATCCCTTTTTGGTAGCTGGAATAAGTATGCAACACCGG AAGGATATAAATAACATAGTTGAATTCCCTTTCCTTAGAATCCGATTAGT TGTATATTTGAAACTGCTACCATCAAGTGCCCCAGTTAAAAATGATTATA CTCGTCTAATGAAAAATTCCAAAACTCAAGCATCAAATATGACATGTATG GTTTTAAAGGGTAAATTGTAGTTTTCAACAATTGGCGACAAACAAAATAA CGGGCAATGATCTAAAATTTATTATTTACCGGTCTAAATGGGTCAATAAT AAATCATTTAAGATCCAACAGATTTTGAATACACATTGTGTATATATATA TATATAAATATTTAGTTAACAATAAAACTTTATAATATATCTATTTCTTT TAAAGGAATTAAATAAAAAAAAAAAATAAAGTTACAACTATATAAATTTA TTTTTCTACCAAAATACCTACAAAGTAAGGTAAAAAATAATAATAATTCG TAGCTGTTTTCTTGTTTATCTTCATAGGTATATATATTAGAGAAGCATAA ATAGTCAAACGCATATATATAAATAAGTATATATATATGGAAGAATCCCT CGAAAAAGATTTCAACACTTGATAAGACCCTCGTACACTTTGATATACAA TAAGAAAATAAATCCAAGTTATGGAAGAATTGTAAAAATTTTTTTTCCCC CTTATTTTAAAGAAACGTTTTTTTATGTCCTGCAACATAATATGACGTAG AGTAGATTTATTTTTTTTCAACTTATTTCGAATTTCAATTACAGTATTAC GGAAAAATTGTCATGTGGTAAATTAATAGCCAATACAAAATTTCATTTTC ATATGATGTTATCATTAAGTAGCACATCTAGTTCAGAGTTTGAAAGGTAA CAAAAGCTCTTTAATTCGTCAATAAGGATTAAAATACAGCATTTATTAAT TAGGGTAGGCATTAGTCTAACCTATAATAAAATATTTATAAAGTGTTTTT TTTTTCTAAAGCTACCCTATGGAGCAAAAAGAAAAAATGAAATAAGATCA TTTTGATTGTTCTTTTCTGAAAATGTAAACAAAGAATGCGCGTTATACGC GGATCGTCAAATTTTCCTAACTTTACTGTTCGATAGTACTATTTTAGATG AAAAAACATTAAACGCATTTTTATAAGTGTAAACTAGGTCTATCATAATT ACTTTATGTAGAATAACTAAAAATGTGCGACCTAAAGATCCCCTTGTAGG GCAATGCAACTTTCCATAGGTCATTTTCATGCCGTATTTACGGACTACTC AAAATCGAAATTCGAAAAAAAATGAAAATCAAACAGCTTCAATATGTAGT GGAGACCCAAGGCAATTAGATCACTTTTTGTTTTAAGGTCAACTACGCGG AACTGGTTTGACTTGGTAGACACTGTTTGAGCTTTTACTTTAAAAAATGA CAACTCACCTGTTATTTAATTTGAATAAAAAGTTCGTGTATAAATCTCAT TAATAATATTACTTTAAAATTCAAATAAAAAACAAACCGTTAAACCTTTT GTTACAGAACTGGGATTTTGTGTATCCCAAATTTTATAAAATTGCCTCTA ATCTGTATAAAATAGATTTTATAACCAGATATTATTATAAAAAGGCAGCA TATAATTGGTAGGCTGTATTTTTTGTTTTTGTAAGTACATTCGCAAAATC ACATATATTTATTTTTTAAGCTATACATATATTTAGTTTGTTTGTGAGAG GCATAATTGTTAGAAGTGTTTTGTGTGTTTGGCGATTTTTTGATTTTGAA AAACATGGATAAAAGAGTTTGCTTGAAACTTAGTCTAAAAAGGAAATTAA GTGCTCCAATACACTTGAAATGTTAACAGTGGCATACGGTGAAACTATTC GGAGTAAAAATAATGCTTATAAGTGGTACAAACTCTTCCAAAACGGCTGG AAAGATTCTAATGACTAGCTTCCCTCTTGATGGCCAAGTACGTTGAAGTA GTGAAGAAAATAGTTTTTGTAATAGTCGACTGACTATCAGAGAAGTTACT GAAGATATTGACATATTGGTTGGCTCATAAACATTTCTCAACTGTATACG ATCAGTTTGTCAGAAAATTCCATCCTCTACTCTGAGAGTGAAATTCTGGA ACATCTGAATTTGTATAAGATATCAAAATACGTGTATGTATCCACATATA TAAAGGAAAAATTGCGAGAATATAAAAAAACTATACTTTTGACAAATTTA TTCAAATATTTAAGAGTCCTTAATAACTCTTGTGACATTAGACTCTGTTG AAAAGTATATTGGAGTTTCTCTTTCTCTTTCTATACTTTATTCTATCTTT CACCGCTTATACATTTTTTTTTCTACATTTTCATACATTGTGAAAAAAAA ATGCAGAACTCTTACTTCTTTTTGGCTGAAAAAAAAGGCAGATTGCAACT GTCCATGGTCTTCCTTATTTATAGTTAGCAAGGTACATTTATTGTAAATA TATATATATGAACCAAACACCAAAAGTTACAAAGAAATAACAACTCTATA TGAGTTCCTCAAAATAAAATAAAAAAGGCTTAAAGTTTAATTATGAACAT ATCACTATACGCCACTATAAAAACATTATACATAGTACTTATTTAAGAGT GTCTGTTAAAGGAAAAAAATATGTTCAAGGTATTTCTTCTTTTTTTTTCT TCATTTCCACCCCAAAAATAACAACAAAAAAAAAGAAAAAAATAATAACA AAGTTTTTAAACACATCTGGACCATATTTATTAAATTTTCACTATAGGTG AACCTTACTTTGCTTTGTGATTTATGGAGTAGGAGATTCATGGAATGCTC CTCTTTACAGCGATACCACTTTTGATCCCCTCATAAAAGTGGATTTTGCG AGCAATTTCCATAATTACGTAGTTCCTTTATTTCATTCCTACCTAGAGTT CTTGATGAATTTCGTCATTAAGTATGACGCGGGCATGTTTACTCAAAAAA AAAAAATAATAATAATAAGGGATCCCAAACGCAGGAAAATAATAAAATTA CCTAATCATTAACCTGGAAAGGACTTGGTATCATCATCATAATTATGAAG GAATTAGGGAGGTATGTGCCATAAGGTATTTAGAATACAGGGAAATATAA TCAAGACCAGTGGCAAGGGCACAGAGTGTATGACACCCCAGCGATGCATA TTTGATAAGGTGACAAAAGTAAATTGTGCCAATTTAGAAAAGAAAACAAT TAATAAACTTGCAAAATATTTTTATACAATGACATTTGAGAAAAATTGGA AATATATATTTCTTAAAAAAAAAACAATTTTCCTGAAATTTCTTATATTA AAAAAGAAGGAAATTTGATTCAATTTTTCAAAAAAGAAAGGTTAAAAAAA TCCCACACACATTTTCTCCAGCCTCACATACACTAAAACGAGCCCACTGC GTCAAAAAGTATTTTGAAAGAGCTCTCAAACCGATGGATCCGAAATTTAA TTACAAAGAGCTATTTCTTTTTAAATTGACATACTTTTGTGAATCCGTGC TCAGAGTATCTCAAATTTACACGTGACCTCACTATTGAAAATCGTGTCAC TCTGCCATTGATAAAGATAGTCTAAAAACAATGTAGTGGCAAGCTGTATT GCAGAACGGGTACTTTAAGTGGAKTAACCAATAAAACTCTTTTTTACATT CTATCCAAAACGTCCGGGGACTTTTATATAAATTTTTATAATTCCTTTTT TTTCGTGGTATTCTTAAATTGTATTAATGTCAAAATATTCATACAGCTCT CCCTCTTCCATACGCCTTTCAAACCGTCACATCGACTCAATAAGGGAAAC AAACTATAACTATCTTAACTTAAACCAAAGAAAAATTAATTTTTGAACTA AAAAAATCCTTCTTTTTCGAATGGCTCTGTACATTCATCCCCCACAAAAA ATCCAACAAATTAAACAAGTTGTAAAGATTCTAATCACATCTTTATTGCA TTGAAACAAAGAGAGATCAAAAGAGGTGGGAAGTAGACTATGGACAGAGG TGTATAAAGTATGTCATGAAAAGCGGGAGCGGTTTGTTCTAATTGAGAAA CTGAACAGGCTTTTTTTATTATTGAATTCATCAGAGTAATTTCAGCCTAT ATGTCCTTACTATAAGCTTAGTGGAGTTTTTAGGGGAGTCTGCAAGATTA TATTTTGGGGAGGGCTTGGTTTATGGATTTATTTAGAAAAAAAATTCAAA TATAAAATTTTTGGGAAAAACAAGGAAAAAAAAATTAAAAGGAATCTTCA AAAAAAAATTTTTTTTTCTTTAATTTCAAAATTAAATTAAAAATTAGACA GTAAATATATATTGTATCCAAAACAACAAAAGCAGTAACAAAATTGAATG GATCTTTGTTCTAAATTTTTGTTCAGAATCATCAAATTTTAGATAATACT CAAAATATTTTCACAAGAAAAAAACGCACACACATACCGGCGTTGTATAT CATTTGAATCTCTGATAATCGTTCAATGTTATATTTATGAGTAAGCATTT TTACCATATGCCAAACATAACTGTGGCCGTTTTTTTGTCAATGAGTATTT TTTTATCTTAATTGGTTTTTTCCTCAAGAGATTTTATTCTGTACCTAGTA CCAATAATCCTGAAGGTACACAAACCACAGTATCAGAATTACTGATGTGG TAAACTATCAAATAAAGATTTGTTTATTCAAGGACTCTAACTAAGCAATG CAGAAACATCAAATTAGTTTCCCAAATAAAGGCATACATGTACACTCACG TAGATAGATACATTATACATAAATATATACCTTCATAATAATATAATGTA TTAATTGTATTCTTATTCAATATCATTTTTTCTTTAAGAAAGAGAGAGAA AAAAAGTGAATAAAAAAAATTAACATAGTAAATTTTCATCTTTTTGTATT CTTAACCCACGCCAAAGAACCTCAATAATAAAGCAGTATTATATTAGTCT GTATTTTGAATGTACCTACATAAAATATTATGTATGACTGAGACTGGAAA GGTATCATCACTAATTACTTTTTGAGGGTTTTTTACATATTAAATATTGG GAGGGTTTGGTGGTTATTTAGTGCCAATTTATTATGTCAATGTGAAAAGT ATCAGGTCGTCAGCATCATTGTGGTATTGACGTGGGAAGAATGATCTTTT TTTTTAATAGCATCATTTGCAAAAAAAATCTCTAGGAGAATAGGAAAAAA AATATGAATAATATAAATACTAGTGATGGAGGATTTCGGTCTGAGACCGA TCTAGGATTAGTCTGCGACCACTATAAATATTTCTGATTGACTGATCCAA TATACAATAATAGAAAATATTGGCGAAACAAAGACTATGTTATCAATATT TCTCAAATTATAGTTGCCTACTTTTTTGAACGATTCGGGCGTCCAGTATA CTTGAATATATATTACTCTATATCTGAGCATTCAACCTTCTTGAATTTGC CATTTTAACTTAAAATAAAAAGTGATTTTTGCAAAGGGAAGAACAGATTA CATATTTCATGACGGAAAATCATACAATAACGTCATTTTTGTAAATCTAA AGCGATATTTTGCTTGAGAAACGTAGCCAAGAAAATTTCCTCTAACTACA GGGTATAGGGTTAATTTTCTCATTCATATAGGAAGGTTTAGTGATTATTT TTTGACATTCTGGCTCAGCCATTTTTTTTTCTTAAGCAGATCATTATAAA AATTTCGTCATCATGAAAGAGGAAAAAGGCAAAAGGCATGCTGAAGTTGA TGTGGGGAGGATTATGCACATTGTAAAGTACTCCATGTGCCTGGTTTTCA AGGTGAACAAGATTAAGTTTGATAGAGAAGATATTTCGAGGAAATCAGGA GGTGAAGTGCACAATTTCAATATGGATCCGGAGTTCCTGGCCAGCTTGGA GAAGGAGATAAAGAAGAACCCAATCAAGTCGATGAATTGTTACGCCAACA ACATCTCCATGGATTCTAGGACCATCAGTAGAGCCGTGAAGGGCGACTTG GGATTTTTCTCATACAAGAGGATACCACACCACCTTCTGACAGATGTCAA TTATTGAAGTGTTTCTAAACAACCTCCCGAGTCCACTTTTTGCTCCACAG CCCTATTAGAGATACTTGTCGTCCCTTGCTTCAAAGGCATTTCCATTTGT ATTTAGTCGCATAGGAGGGGTAAAGTGAATAAAAATCTTTAGATTTCGGT CCACGGTCTCGGACCAAGTGTCAACACTAATAAATCCATATTATGTAGAA CTCAGCTACCACCTTCAATTCCACATTAGATCTGTACAAAAAACATTTGA CTCACAGCATAGGTAGGTAAAGAATATTAAAACATTGATGAATGTATGAA GGAACTAGAAAATCCTTATAATATCTACGCCTTCTCGTGGGTTGACTCCA GGGTCGAACGGGTGTTTTCAACAAAATAACGATTTGTTAAATGATTACAT ACTACATAACAATAATTTGGAGAAAGATTCATTATATTATAGGGAAATAC TTAATTTTGTTCCTGTATAGAGAAGTGATTTGATTAGGTAGGTATGTTGT GTACAAGTTGGAATTTTGTACAAGTTGCAATCAAGAAAGGAGACGATTAA CTATTTGTATGGAATAAACTATAGTAGTTTTGGATAAGATTATGCAATGA TTGAGCCTCTATAAAATAGTGGATWTATTAAGGATGCTTCTTTTTTTTTT TTTTTTTTGTTTGATAAAAATTACTAATTAATTAACAGCCCATGATCTGT ATTTAGAGGTCCTATCACTTATAATTAATTATATTATTATTATGATAAAA TGTATAGATAAAAATGAAATAGGAGCATCAATTATTAATAAGTGAAAACA AGGTTGTGGCTTGGATTGTTTTCTTTAGGGAGGGGGGGGGGGGGAGTAGG AGTTGTTTGACTGCCTCATTATTTTTTAGATCTAACGTTGGTTTTTTTTA TTAAATGACTTAATTTCTTTGAGAAAGTATTTAGAATCAGTATATTATGA TAAATAAAATTTTTTTGTTACATAAAATAAAAATGAGTAAGGAACATCAT TCATAATTTCACCTATCGACAGCTTATTTCGGAAATTGATAAGCATTTTG GAATTGTGTGATTATATTAAATATCTACCATTTGATCAAATTTGAGAACA TTAATTCATTTTTATGAGAGGAATGTTTTGCTTTATATAAAATATTCTGA AATTTGACAAAGATTAATTAGAATAATTTGTCAATATAAATTGACAATAA TTTGTCGGATAATACAAATTCAGGAATCTTTTTTAATAATCATACTATGG CCATATTGTTTTTTCTTTTACCCTCTTAATCGGCTCTAATAGTGAAATTA TATATCCGTGTCAAAAGTTGTTACATTTGATCGAAATTGAGCTCATGGCT CTTAAACTTTTTACTAAAATGCTAAATTTTATACCATTTATTTACAGCAT AGTATTCATATATCGATATAATTTATTCCTGTGTATTATGTATTAAGGTA CTCTTTTTGTATATGTCTACTTCTGTACATAATGTATCAGTTCTTTTTTC AATCTCGTCAAATCACGTGGCGGAGTCGGCATTTTAAACCAAGAAGAGAA GAGTTCACCAAATAATGAGTCTATTTTATAGATAAAGTACTTATTAATAT AATTAATTAAAAAAATAATTAAATATTGAAAGTCAAAATATTACAGAAAC AAAATGGAAGGTTGAGACAAGCAAAATTAAAATATTAATTTTTAAGGCCC ACCCTAATGGCTACCCTATCAATATATTGAATATGCAGAGTGGACAAGCA TTTGTACTCAAAGACATTTCCACACCCATGTATATAGGCTTAAACACAAA GAAAACTGCATTTCTTTGTTTTATTTACACTGATGGTTATAAAATGGGGT CAGCCCAAGGATGTCTATCCATAATAAATAAGTAACTGACATATTGANTG CATTTCTTGTTTTATTTACACTGATGGTTATAAAATGGGGGTCAGCCCAA GGATGTCTATCCATAATAAATAAGTAACTGACATATTGAACATGACATGC AGGTTTTTCCTTTCAAATAAATAAATAGAGGGTGTGAGCTTTGTCAATAA TATATTATTTATGTATACGTCATATAAAAATATTGTTTGCTGACTGGTGA GACAGCCAAGGAATGACATTTAAACTTTTTGTACAAATTTCTTCAGAATT TCTTTGTCGTTTTACTTTTGAAAAAAGAAAAAGTAAAATAATATCTGATT GACAAAAAAAATAAACTTATAATTTATGAAGTCCGTCACATTCCAACAAA GAGAGAGTATAATCAAGCCATCCAGTAACAGAGTTTGCTTAGAAAAAGAT AAAAAATCCTTCTAGCCTAGTTTTCTAACTATATACAATTATCTACTAAA AAAGTTGTGAATTTTTTTTTCAAAAAATTTAATAGAAGTATTATCCAATA CATAAGGCTGTGAAATGGATTTTTAATTGTTTCTTTTTGCCAAATTATAA GATATTTTGACAAATTTGTGATACATTGTTATTTTTTTTCATGACCTTTT TTTATATAAAAAAAACATGGCATGATAACGTCAATATATAAATATTGCCT AAATAAAATTAGACAACAAAAGTAGATCGTAACTACATGTGTTAGAAGCA GACCCTGCTTAAAACACAAGAGTCAGGACTCAAAGTATAGAGAAAAACAA CTTGCAATTCCATGCACACAAGAAGTCGACAATTTTAAACTTGCATAAAA TTGAATGGGAATGACAAACATTCATTGTATAGAAGTTTTATATCAATGAC TTAACCTATTTATAACATTGTAATCACGTAATTTTCTATTTGTTTWTATT TTCAAAAAGTGCTAGAACCATACATGAACCATACATTGGTCCCTATTTCA GCGCCATTTTTGAATTTAATTNGATGATTAAAATAGAATTGGAGAGTCGT TGTGAGGGTCGACCTTAAAAATTACAGAAATATATGCAAAACGAATTTTA TGGTGCCCAAATTTAAATATAAGATTTGTTAAGGATAATAAATGAGGTGG ATGCCTACTTTAGTGTACGCAGAGCATGGAAGAAATATGTATAAATTTGC CAAGCTCCATTTTATATAATATTAGAATTTAAGAATCTTTTTTTATATAT AAAATACGAAAGACATTTCGCTTTTATTAAATAACCTTTTTTAGCAAACA TAATACTTTAGTAGCTATCACGGGGCCTATTGGAAAAACGGGGCATACTA TCCTTGTAGTGTACTCGTATTTACAATTAGGATTCGAGTCCAACATAAGT CGAATGTAACTTCGATTTCAGACTCGAATTGGACACTGAGGGAAAAAACT CGGGACTATACTTAAGAAACGAGACTTGAATCTGACTTTCAACAAATCGT GTGGGGAATTTGTTGGGGTAGGGCCCCTGAATTTTAATATTTATAATATG AACAGCTGATCAATACTTCCCAACCCTTTTTGAACCGCCTCCTGAAAATC TTTTAGATATTTGAATAATATGGTTGCAGTAAAGTTAGGACTGGAAAAAG TTCTACCCACTTCTTAATAGACTCTGCTATGTACACCTTTACACAATAAC AACTTATATGTTCTTACAAACCCCTCTTTAATGCCATCAGCTGTTATTTT GTTTACTTGCTTGTTTTTCTTACGATTATTTTTATTATTTGTTTCAAGGG TTTAATTTGTAGATCCTCACTCCTGATGTGAGGGGCACAGCTCAGAGTTT TGTAGGGATTCCTTAATTCTAGGGATTAAGAACTTTTTTAGTGTGTGTTT TATTTTGTCATCCACTGTGAAAGACTTACTGTTCTTTGATACCTTTATTG AACTGGATCATCAAGGATATACGGGTCTTCATAATAAGTCAAGATATTAC CTATATCAAGTATAGTGGGACACAACTGGTTTGCCAACGGCGAAACTGAG AAAAGCAAGTTTCCCTTAAACCGTTCTTCAAAATTCATAAAAGAACCGGT TCCTGTAAAATTAACTGGTTAGTGAATCGTGAATTTGCACTGACCAAACA AAAAGAATGAAGTGTCACCCTGTAGACAAGAGAAAACTAGGTCAAGTACC CTTTACTCCGTGGCGACCCGTGGCTTTTTGGCGTCCTAGGCAAAATATAC TATTTACCCATTAAACATTCGCGGTAAAATAATAGATTACTTAATTTTTT GACATCCCTCTTAGGATGTCGCCCTAGGTGATCGCCTAACTCKCCTATAA CAATAGGGARTAGCCTGCATCTAGCCCCTTCCTTCTCGTTCCTGTTGTTA AATAAGAACAATTTTTTCCACAGAAAATGAAGTAACAAAGAACCAGATCA GAACTYCTAATATTAAAATGATGATCCGTTTAGAATTGGGAACTGTTCGA GAAAGCAAACGAATAGAACCTGGAACTGCAATTGAACCGTCTGTTACATC ACTATAATATATATCCAAAAAGATGTTAAGATTAAATCAATGACTGCTTT TTTTCTTACTATAAACAAATCTGTAATCTCTTTTATTATTCTTATAAATC ACTTAATCTCGTCTCGTGGAGCCAAAAGTCTATATATTTTTGTTTTTGTC GGACATTTTCCTTTGTTTTAATAATCCTGTTATTAATGAATGGCATGATC ACCCATAATTATGAATCAATTCTTCGTGAATTAATTGGCCCTACAGCATA AGTTATTCATCTTTTAAAGCACAAGAAAAAAATCATAACATCATTTTACA AAGAGTATGTACATAGAGCAGAACGCAATTTTTTCATACAGTTTTATTTG ATTCTAGCATTATCCGAGAGGTGAAAGGAAAATAGATTATGTGAGTGGCC AAATTTGCTTTTTTCACTCCTATATTAAGATTTTTGGGAATAGGGCTTAA AGTGAGTGTACAATAATCAAATCTTACCGATGCTGAAATTTTAATTTCGG GGAAATGTGCAGGAGCACCTAGTAGGATCAAATTAAAAGAATACTATAAA AATATTTTCAAATTATAATAACAATTATTGACGTAATCCCGTGGTTAATT GATGTCAAAAAGCTACTTTTTTTATATCCATGATTTGATTTCCTTCATGA ATATTCATGAGCGACCACACAGATATATTTATATAATATGTACAACAATA AAAGCAAATTATTTCTTCTTGGACATGCCACTTCAAGTCTCTTTAAAGAA ACAATGAATCTGTAGTACTGAGACTCCAAAACCATTTGCATCTCCATATT CATATGTAATGTTCAAGTATATATAATTTAATTTTTGCAAAAATACAAAA GAATTTATGAGGATAGGAACTAGTGTTGGGCTTCGGTTCGAGATACTTTT TATACTTTTTGTTAGAACAAAATAATTAAACCTTTTAAAAGAAATTCGGT CTGGACTGGTCACATTCTTAAAATTATTGTATCTTGTAATCTTTCCTCWA AAAGYAAACAYAAAAAAGGCKAAAAATTATTTGMTAGCCATTAATAAATT CTGAGCTTATTTGTTCCAAATATTGAATAATTACATATTTTATGAAAGAA GAGAATTAGTTACAGCTTAATAAGTGTGAATTCTAATTCAACCAATCAAC GTTCAGATTACTGTTTAGAGGAAAGAAAAAAAATTAGATAAATAACTGTT TGGTGACGTCATGAGTATTTCTAAATTGTGAGCATTGGTATAATGACGTC ATATGGAACTTAAAGTCTTTCTATGTGTAGAACTTTTAAAACTGGGATGT GAAGAAAATCGGTCAACAGCACGGCTTCAACTTTATTTCATCACACCTTC TTTCATACTAAAAGAAACAGAAAGAAAGGCCAATATTCAGTTGGTAGCAG TTATTTAATTCTTATCAACTGTGGAATTATTATTTAATAATAATATTTGG AAATTGTTCTCAGTTTAACAAAAGTGTATTCTAATTCAGGCAATTAACAT ACAGAAAAATGATAAGGAGAAAAGAAGTGGAAAAATAGACAGCTGACAAA AAATCCATTGTTATTGTTTTGTGTTAGTAAAGTATTACTGTGACAGAGTT AGATTTTAAAAAAATCGGTCCATATGGAAAGACTGAAAAAGTCAGTCTTC GATTTAAAATATTCCATTTCGACCCCAGGTCTAGGATTGCGATCTCAGGG AAATATATGTAGGGTCAAATTTGTGTAGAAAAAATCACATAAGATTGAAT CGAGGAAAAGTCGTTTTTAGACCGAGTCTCAACAAAAATAATAGCCTTTG CATATTCGGCCCATCAAAGTGCAAACTCTGATAAAGGGAAAATAAGTGGA GACATTGGCAACGGAGAACAAATACAATATAAATTTTGTGTGTTTGTGAC GTAATGCTGTTATAGAGTAAAATAAAAAAAATGGCCCAAGAGGGTGAGAC TTAAAAAGTAGACCCACAATTTATAAGGACTAAATTTCGATGTCAGTTCT AGGATTTCGATTGGAAATATATGCTCAAATGTGTCTACAAAAAGTAACAT AAGGCTGAAAACTCGGTCTTGGACCGAGTCTCAACTCTAATAGTAGTCTT TGTATATTAATTATAAACATAAGTGACTTCTTCTCTATATGATGGACTTA AATGAAATTTGTTCATGAAAGAAAAGAAGAGAAGGACTTGTCTGGGCATC ATAATTATGTACCCGTTGTATAAATCAATGTAATTATACAATTGTGCAKA AGACATGCCATATTGACATTTGCACACTGTATAAGAAGCCAATGTCATAA ATTATTGTACATCCAATGACTCTTTCAAAATATAAAAAAACACCCAATAT CCCCATTACAACAATAGCTGAGTAACTTTAACTCATTTCTTCTTGAGGCT CCTACTACATATGTAAGTAGAAGTCAAAGGGTCAAGTCCCCACATATCAA ATTATGCAACGTCATTGCTTTAGTTTGAAATTACCAAAGGGGAAGGTGGC TTACGAAACGAGAAAAACAGAGTGATATTCCGTATGTTGTAAAAAAATGA AATAAATACAAAACAAGCAACGGAATGAAAAGTGTTTAACAATGAAAAGT AATTTGTATAACTATTTTTGGTATAAAGAAAGAAAAAAAAAGAAACTAGG CGTCTGGAGTATTCACATTCAATGACGACAAACTTTTGTATGTATGTACC TATAATATGTGTGGTTGTACCTATGAATATATGTACCCATAAACCCTACA TTCATAGATGGTTCCCCGTAAAATTAATATATATTCTTCATTTCTTGTGT GCACCTATGTCATACTCATAAGGAGCTGTGAATGGCAGACAATTTCACTC TCTCAAAATAATTGTTATCACCTAAATGGAAAAATGCTTACCTACTTATG TGGCCCGTCAGAACAAAAGTAAAAAAGAATAATGAAGCTAAAAAATAAAG TCTGGATTACATATTTTTTAGTGGTTGACAAATTATCTTATTAATTCATC AGGATTGAAAGTAAATTGCAATTAATGGATTCATCTTCACAATGAAAGGA AATTGATAGATTTGTTTTGAAATGGTCCATATCAGGCTCAAAGTTAGTCT CATAAATCAAATGAAGATTTTTAATCATACCTAACGGTCAGGGGTATCTT AACTCATCCCAGAAATTTGAATACAGCAAATAATTGACTGAAAAAAGTTC ATAAAATATTGGGATGTACACATATAAATATACGGTTAGTTTTGGGTCTG AAAATCCTTGACTGATCTGAGACTGTTATGACTTTTAACGAACTAATTTA ATCCTTCAAAGAATTTCGATCGGAACCGGTTTAAAAAAATTGGTTTATAT CGGTCCCAACAAAAAATCAGTCCGTATTGTTCTTACAAAAAAATCCCTCC ATTCAAAATTCGATCTATATGGACCGATTCTACAGATTTATTGTTGATAA AGTCATGTGCCTTTCCAAATTGTGAACATCAGGACAATAACGCAATATGA AGTTAAATGAGTTGTTTAAATTGGTCTATGATTTGATAATGATTAAATTT TCGTATACAGTCTGGACCTAAATATTTGTACAAATCAATTGAAGGAAAGA CTGAAGGGGGGGGGGGGGGGCAAAAATTCGATCTTGTACCGAGTCTCAAC AATAGTATATTTAATTCAAAACAATTAAGTGGACATAATTTCTCTTTTTC TTAACTTTGGTTAAAATATTTTTTTGTTTTAATTCACGACATTTGTATCA GTTGTGTACATATTATAATTTAATTGTACATTTAAAAAATATAACTTATG CAGTGAGTTGGACGTCTCATTTTATTTCATCAGCCTTTCTAATACGAAAT ATATATACAGTGATAGTGTTATTAGTTATATGGTTCTTGATTTCTGCCTT GGGTCTCGTCTCGGTCTTGTCTTGGTTTCACCTCTTGAATGTCTCAAATA ATCAAATAAGGCACAAATAAAAGATTAAAATATTTTTTTCCTTTAAAAAA ACTAGAATTTTTCCTCTTATTGTCACGATCCCTTAACTTGGTCTCCCGTA AGGTGGTTTACTGAGTGTTTTCATGTTAGGTAAGCATTGTGAATGTTGGC CTTTTTAGAGGTCTAAACAATCCAGTATTATAGGAATAAAAACTCTTTTT TGATTAAAATTAAGAGAAATGTTGATCGATTGACTTGTACTTCGATGTCA AAATAACAACAAAAAACAAAAAGCCTTAATCCCTTTCTGCTATATCCAAC AAATATCTCTCTAACGTTTAGTTTTATTGTAGCATTATTTTACATTCTTA TTTACATTGTGTAGATAATAAAACAAACTATTAGAGTCGTAAGGGTAAAA TATTTGTATTCAGTTATCCAACTTTATTCGTTCGTAATCTATCAGATACA GAGATAATTAGCAGAATGAAAGCATTAAATAGCCTTTATCATCTCTAATT AATTTAATTAAAATCAAATATCACTGCTCATCATTGGCATCAGGTAGTAT TCAATACAAATCGTAAGTCTTTTTCAACATTCAAAACAATTTGATGAGGG GTCATCAACATKAAATATGASCCGCCAATATGGGGTTGCCTTCAATTTTT GATGTCAATGACAGCATTATATTATCATTTCAGTTATATTTGATAAATAT TACCAAAATATATACGATATATAGTACTTTGGACACTTGAATTGTCCAAT GTGCTAAATTACAACGCCGATTTCAAAATATTCAGGACTTCATCAAAAAA GGCATACGGGGTATTATTGTCTGTGAAATTTACAATGTCTAGCCTTCTAA GTCTTAAAAATATGGGGTTATTATTTTTACTCTGAAGGCAAAGGTACTAA AACTGTTTATAAATTAAATATTTTTCATGCGAAAAGTCAAAAACTATATA CACAATTAATGATAAATGTTCAATCCTCTAATGGGCATTTAGTCATTGTA AATGTTTTAGTTACAATATTTAGTGTGACTGTATTTAAAACAACAACTAG AAACACGTGGACATATTATTGTCAATTTTGGGTTTTGCTTAAATAATTTA AAACTAATAAAATATTTTTTTTTTGCAAAATATATTTTGTAAAAATCTGT ATTTTTAATTACTTTTGTTTAGATTTTGGTATTTTTTCATTATAATTTAC TTTTATTTTAAGTCAACCTTTAAAAATTTGTCCGACATGACATTGAAAAT TTCAATAATTTATCTTTTATTAAAAGTCCCCGGAGAGAATGTAAAAAGAA GAAAAGTTCGGAGAAAATATACCTAAAAAGAGAAATATATATTGGCTTCG TCCTAAATCTTAAAGACGCGTCTGAATCGGAGGCCAAGGCTGTCCCATAA CACAATCTTTACTTAATGAGATCATCTTAGATTTCACCCATTGACATCAT CATCAATCAATCTTTCCCTATCTGGATTCAATAATAAATACTGTAACTAG GTCTAAGTTAAGAGTGATAGCAAACTAGCCCCTTGCATGAAGGATACTAA AATATGTACGAAACTAATTCAATAGAGTATGTGCAAACTACTAAAAAATA TATATATTATATGCAAGGTTATTTAACCGTCTGTAACTTGTTGTTTTTCA AAAATGATGCATTTTTAGAAAAAAATTCACAGTGCCATTTCTGTAAGTAA CATAAAAGTATGTTTTTATTATGCAACATTATCATTCGGGGAGTTGTAGT ATTTTCTCGCTTCTCGCAAGTCGGTGCCGCTCACTGTGGTTTTGTTTACA ACCTAATATAAGTAAACAACGTTCCTGCCCAATTATCAAACTGATGTAGA TACGTTACTATACTATAGGTAAAAATGAGGTCCTGTAGAGGACACTCTAA AATTTGGAACGTTTCTCCAAGGAATTGTTCGATTTTAATGGACTATATCA AATGCAACCTTACTCTACAATATACATTATAGATCCTTTCAATTTTTGAT TGCAACTGGTACAAGTTGCTTGTTGTACAAGTTAGTGTTTGTACAAAATA AATCTATGATTTAAGAAAGGGGTCAGTTGAGAGGTTACGTTGAGTATATA ACAAATATTATGTTCCATTACTTTATAATTCAAAATTTGAATAGGTATTT TAATGTTCGCTATTCATTATAATATCTGAAAGATCTCTCAACATGAATCA TTGTTAAAGTCCCCTCACGATAATTATAATTTCAAACTAGATTGATCATA AGGGGGTATATATGTTACATATTTGTCTTTGTTAATAGTCTTTGAGATTA TCATTATATATGTTGCTTAATACTTTATTTGTTACAATATTATAAATGAT GTTATTCCTTTATTAGAACACAAATACATATTTGAACGTATTTCATTGTA TTTTTGATGTKAATAAKAAAGAARWMTTTTTCCTAWTCTTTTCCTGGNWT TTTTTACTARCCNNNNNNNNNNTTCACCAAAATATGCTGAAACCATGCCC AGTTTCACCAACCAAATTTAAGCCTTTCTTAAGTTTACGACAGCTGATAT TTTTACGTCATAAGCCCTAAGTTTACATTCAAGCCGGGATAGTTCAAATA GCCCGTGGATTTTTCCAAAAATATTAATTTTTTGAAAACAGTTGTGAATA TTTGAAGTTTTTTACCAATTAAAAAGAATTATAATAATTTTTGGGTTTTT TATTCCAAAAAATTCCAAAAGCCGATATATATGTATAGTCCTGCAGACGC ACCTGGTGATCCTTTTGCTTAAGCAAATAAATGGTTGTCAGTTGCTCTTT TAAAAAGCATATAATACCTCTACTCATCTCATATATAAAGCACTCCTATA TGTAATTAATAAAAATAAAAGATTTGAAACAACACGCAAGTGGTTTTTAT TTAACATAATAAATATATGTATCATGCTATAAGTAATATACATAGGAGTA TGAGTTTGCTATTTGACAAGTCACAGCTGTGAGTAAAATATATGTATATA TATGAGTATAGCAAATCTTATCAAAATGCCCATTATTATCAAAAAATTAA TTTTTATTTTTCTAAAATTGCAAAAAAGAAAAAAAACTTATCATTTCTTC GATTACATTAAGATAAATGGAATTTTCGACTATATAAATATTATTTATCA GTTCTGTAAGAGGTCTTCGTGACTGACTCTAAATGGACTTCCGTCATTTC ATTGATTCTTAAATGAATATACAAGTATTTTTACCGTACATATTACATTG TTATTATGTATGGCCCTTATCACTAAGACAAATAATAAAATAAAAAGAAT ATATTTGCAATGGTATATTCTAAAAATCAACTACTCCGGTGTATTTTAAG TCATACATCGAATTGTTTGATTGTAAAGCTGTGAAAACCCTCCCTAATAC TATACTCTAGGGCAGGGGTTCCCAAACGTTTCAGCCTGCGMCCCCCAAAA TAACGGTGCCAGAGACTGGCGACCCCCAACAACACTGGAGGTGGATTATA ATGTTGCGCACAGCCACGCGCACATGCACTATGCGTGCGTGCGTGCGTGC TTGNGCGNGTCTGTTCTCCCAGACAGCACGCGCACACACAGGCCACGGGG CCCCGCTCACTCGACCAGAGAGACACACACAGTGAGATCAGAGCKCGTTC ACGKGAASCAGCCGATCAGTGACTGACTGCTTCTTGACAGTTGAAACAGT GAAAACACAGAGTCTCTCTGGATGCTTGGTTTTCAAGTGTGGGNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNCCCTGCATCTGTGTCTCGCGACCCCCACTTTGGGAACCCCTGCT CTAGGGCAGTTGTTCTCAAATTTTAGACAGTTTCAGTGCACTTTCAATAC TATAGAATACTTAATGGCTCACCGTATATAAAATTGTATTAATTTATTTT GTAATTGAAGAAAGGATCTGTGTAAAGAAAAAACGAATTTCAATAAAGTA GAAAAGAAAAAAAATGTTCATGCTTGTGCTTTGTATCCTTTTTGTTCATT ATTTAATCAGTCGCGAGGGTTTTTTTCCCTCTGAAGGAAGAATTTTCGTC TATCTTGGGTGTAAATTGTTTTCAAAATGCATAATAAAACTAAATATAAT TACAATATACAAAGGCTATTTTAAATAAAGAGGAAACTCTTTATACCAGT AAATCATTTTCKCCCTACAATATCAAAAAACAAGCCACTGATATTTAATT CATTAATACCATATATCTCGCTCTAGTCTTATCCTTGCGATTTTGACGCA AATCCTATCTCTACTCATAATGAGACAAATTTTGTTTATCTTTTTGTTGG TTAATTGGTTGTCGGAAAAACTTTCTTTACAACGGGAGTTGTAAATTTAA GTTTTGTTGTTTTTAACCAGCTGAACAATATTTTTATTTTTTCAAGCTGT TATGATTATTTTTTCATTCTTGAGAAGAATATATCCAAGTATTGAGTAAC TACAGTGAGTGTGAGGCTATAAAATGAGTGTAACACTAAACGTTTGTTGG GGAGTTATTTTTTATATTATTGAATCAAAATGTGTCTGTTTGTCGACACC AAAATATTTCGGGGAATGCGGTGTACTATTGTTAGTATCTTATAGTTAAG TTAAATATATAGAAAAGTTATATACGTGTATGAAGTTTATTTATCCCATT GTTAATGAGAAACGTAATTTTAATGAGATCTCACGATTTTTCCAATGTTC ACTCTTCAGCCTATTGTTTGACAGAAAAGCTATTTTGTAGTAATGTTATA TGCATTCAGGCCTCCTACCTTTGATCCATTTTGAATTTAGAAGTGAAGGG GTATCTGAGGGGCTGAAGTATATTAAGGATTTTTCCTTTAAAAGAAAATT TAATATTTGAAATTTTATTTAATTTTTCAAAAAAAAAAAATTCCAAAAAA ATTATTTTTCTATAAATGGTTATGAATTATATAAAATTTTATCGGAAAAT TTTCTATTTAGATTTTTTTTTCTTAAATTTTTTTTTTGAAATTGTTTTCA AAAAAATTTAGTTTTTGTGACCATATGAGCATTTTTAAAATTTATTTCAA AAAATTTGGATTTTTAATTTTTTTTATAAAACAAAAATATATTAAAATTT TTGTAAATTTGTGAGTTTTTTGAAATTTGTTTCCAAAGAATTTAATTTTC CCTTAAGAACTGGGAATTTTTTAAATTTTTTGGATTTTTTTTCCTGAAAA GTCCAAAATTCAAGTCCCCCAAAATGTAATCCTGCATAAACGCCCCTACA ATAGTGAATATATATTTACAATTATTATTTTGAGAGGTCATATTATATGA TTGTATTAGCTATATATATATTTTTGTTTTGTAATGTGTGTTGTTCATTA TAAAACTGGGCGTTCCTTCTTATTAGATCTCATTGGATGTTTTGACTCGT AGCTATTTCGTTTTATACTTTCAAATCTATATACATTAACTTTTAATACA TATTATAATTTATCGAAGGGAGCACCATTCACACACCAAAAGTAGTATAA AGAGACTCAATACGTATATGATATAATCAAGTTCAGACATATGTTGTTGT TGATTTGTTTTTTGGATTGTAATTATTATATTTAAAGACATTGTTATTCT GTCATTTTGTAATGATGGAAACTCCAAAAAAGGCATTTTTCTACATAAAT AAGTTATTATTCATTAAATATATTTTTATGGCGTTTAAAAATAGTGTGTG TTTTTTTGTCCATTCAAGTGTTGGGACAAGTTATGGATATATATCTATAC ATAATTTGCAAAAATCTCATCATCATTCAAGTAGTTGTCTGGTCTGAAGC ATTGGTGATTGAACTCGTTTCATTGAGAGAGATAGAGAATAGTGCTGTAG GTTTGGTCTTGATCTCGACCCCCCCCCCCCCACACCTCTCATATTMTCCT GATCTCTATGTTAGTTAAGTTATTAATGTAATTTATCATATAACCCTTCA CAGACAAAACAAAAATAGGGAAAAGGCTCAAAATCAGTTAGTAGTGAGTC AATTCCGGGCCAATTCTTTCGTAACTATTGAATCATCATTCAATAATTGT TCAGATTTGGTCACAGGGCACAAATATAAGCGTATATTACACACATAGAT AGCACTGCGCAAAATTCTTTTCAGCATAACAACTATTTTAAAGAACAAAT TACAGACTCTCAGAACCTTGTAGATAATATATTTTTTAATTTACTGATGC CCTATTAAATACAAAGCTTTTTGTCTGAGGTCACAAATGAACAGACAGCT CCACCGAGGAGGAGGATATGTACTAAATTTTATAAGATAAGAACATCCAT TTCAGTGGCACTGTCTATTGATTCTGATGCCACAAGTAAACCCTCTATTA AAATCATCTTTTTCTATCAATAAAAATAGAGAAGAAAAACATCTATTGAT CTCGACCTACAATGGTATCTACCCATATTTACCCTTATTCCCGATACTCC AAACTTTTGGTCTCGGATAAAGTGATTTTGAGTACAGCACTAAAATATGA GAGATAAAGAGTAAACGAAACACGCTCCTTTGATTACAATAAGAGTCATA ATACATATATTATGATATTTCATCATATTCTGTATAAAGTGAACAAAAAA TATCCAGTTGAGGACAAAGAATGAAATTGTCAATCTATCACGTCATTACT TCAATAATATTAAAGTTTACAGAGTACTACGTTATGAGCGGTCGATTTTT TTATTCACGGTTCCATAAAGTACAAAGTAATTACGTAATCATACATTATA GTCTTAATTTTTTGGTTGAAATTAAAGCTAAATTGTTACTTGATAAATAA TATATTTATCCTAAGACATACATTTACTTTTTACTATTAAGACATTTAAA ATAGAGAAAATTTTAAACAATGTAGATTAGAATTTGTAAAGTTTTTAAGA ATTGACCTTAAAATCCTTTGATATAAGCTATATTCATAAGTATCCATGAG GTGTCATCAGCGTTGGGATTTTTCAAAGAATGAGATGTGTGTCTAATCGC AGTGTCCATGAAAATTGGCTCTTAAAAATCATACTTCTTGCAATTAATAC TAAAGAAATTAATAGAGTAAGATCAATTGCCTTTTTGGAGGAGACTGTTT TTTTTTTTCCTTTTCGATATGTCATAGAGTTTAAATGTTCTCATTAAATT TGAAGATCCATTTTCCTAACCTTTAACGTTAATAGTGACAAATTTTTACA GCATTGTAACATGCAAAAGGCATGTAATATATCGTTGAAACCAATTTCAT GATCCCTATCAGTCTTTTGAATGTTGATAAGTAGAGTTCCATTTTACCTC TTCTTGAGGTGCTTTGAATAATTTTAAACAATATTGATTAAAGAAAAATT GGACAATAATTTTTTTTTAGATTTATGTACGTATTTACCAATTATTTTTG GGGCTTTTCCATTTCTTTTAGTAGGAAGGGTTGCAAGGTCAAATAAAGGT AACAATAAAATATAAAAATATACACTGTATTTCAATGCCCGTTGTCGATT TTTTTTTTCTCCTTGTTGCTTTGTTTTAAAAGCTGATTGGTAGTACCCAA AAAGTAGCCGCTACTTAGTTATGAATAATCCCCATTTATCATTGAAGAAT TATTCCATATTTAAGGAAGGTCAGCTAACCAAGTTAGCAATTGTTTTTGG GCATTTTTACATTTCTTTTAAGGTGAAAGGTTACTAGCTACAATCAAGGT AAACACGTGACATAAAAACTTACACTGTATTTTGTGTTCAGTTAATGGTT TTTCTTTTATTTTTTTCTCTGTATCAGTGTGTGAACATTAATTAATCAAA TTTGACTTTGCTATTGTTTAGCTTTAGACAATTGTTGCCGACAATTATTG AATTARGGTTCTAAAGTTATGAAGAATTGTCCAACAAATATCAACTGATT TTGATCCATTTTTCTGTTTCTTTTTATAAGAGAAAAGGTTGCGAGGTAGA ATAAAGCTAATAACGTTCTAGACTTTTACAGTGTAATGAAGGATCCCTAC GGATTTTTTTATCATTAAATAATATTTGAATTATATATTTGAGCTGTATT CACATAATAAAATTCATTTTTTTAATTTATAGTCGGTATTGAATTACAAA AATACATAGAACAACGATATGTGTTATTACTTTTTAATTAAGTTACTTTT TTATTTTTTTTTCTAATACTAAAACATTGTAAAATTTTGCCTATTATTAT AACATTTTGAAGACATCCATCATCAGCATTCATTTATAAAAGTAGTCTAT AGGCAACCATCTGTGATTCAAAATTACACAGACGACATCACCATTAACTT GAAATCTTTTATTTTTCTTCTCCCTCTCCTTTTAAATAATAATCATTCTA AGATATTCATATACATAATTAAGTTTGNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGGAGCTGCTCTG ATATTCATCTTATCTATTATATATGTACATATAAATAAATATATTTGTTA GCAAATTTGCAAAATATATATATATATATATTAATGGGGACAGATCTTCT TCACCATAAACCAGTCAAGGCTAAATATGTTAGTAATTATCGTACCATAG TTTTTGATTAGAACGATTAAAGGTATACTACGTAATATCTATGAGTCTCT CTGTCTATCTATCTTTATATTTGGGACGTTAAAACAAAAACAATACTGAA CAGAGATCAAGAAAGGGAGAAATGTCCAATTGGGCATGAAGTTAGTAATA AAATACATGGTGTTAAGATTAAATCGGATCATTTTTCAAAAATCTTTAAA TTGTCACTGAAGCCACATTTTTTTAGCGTATTTACTCGAAAATTGGCTTG AATTGTAAATTTAAATGTTGAAAAAGAATTAATTCTCCAGCATCAATCAC AGCCTGGATGCGGGTCCGGAAGGAGGAACAGCCGTTGACGAGGTAGTTAC TTGGTATGTTGGCAATTGTCTCCTTGATGGATAGAATCAGAGAGTCCTTA ATGACATGATATGTTTGATTTGTTTTAGATTCGACGTAGTCCCCGACAAA ATAATTTATTGAAGGCGAAAGTTAGTTGACCAAAATAATAAGAGTATAAT TATTTATAGGTACATCAATCACTCAAAATCTTGAATGCAATCTCCCAATA CTTCTATCAAAACTGAAGAGTTTCATAAATAAAAATATAATCAAATGTAA AATACAGGGAGAATCAAACATAAAAAGATTTAAAGCGCCCTTTATGCTGC TAACATTGCTATTTATTACTACTGCAATTGGAATGTTTTTGTCAACCTCA GACGGCGTGAACTTTTTCTTCACATAATCTCTCTATGGGTATGAAGCTTG TTAATAATTTATTTTAGGCTATGGTAGTGTTGCCACCTTGTTTTTATACT CATTTATTGATAACGAAAATTTAGTACATTTGCGCCATTTTGCAGTTATT TATGTTTTTTAGATAACTCATTTCCTATTATGTATATACCTACATACAGT CCAATATTTCATTAACATTTTTCAATTAATCATCAGCATTGTATTCAAAT TGATGAGATGAGTGGAAGTACATTTCAACATTTTCTATAGTTCAAAATCT TCATCTGATTTATAAAGCTTAATTTGATCCCGATATGGCCTTTCAAATAC CAAACATCTTCTTCTAATTTATATAAATTGAAACCATAAATTATTGCTAC TACTTTAGAAACTTTTGTGCAAAATGTTGTATTATAAGATTTTTTTTTTG AGATGGTTGTATGTGAGTGCAAGTATACATATATAAGTCTGACACTGTTA AATTTATGAGTTGAGACCCCCAATACTTAAAACATAATATATTTTGAATG GGTGGAGTAGTACTAAAAAAACTCTCTAACTCAACGAGGAGAGAGATAAA AAAAAATAGGGTTAAAACTACTTCACAATCGACCCATATCTTGGAATCAT CATCTTCTTCTTCCAATGAAACAACTACAACAACAAAACCATTCTAATTA CGTAATGAATCTTTTCTTCATCTCTAGGGATGAGAACGAGAACGTTGTTG ACAATCTCTAAGTAATGAAGCAACGATTATCAAGTCTATAATATTCATAA CTCAAACCCTTTCTTATCTCCTTGAAAATAACAAAAATATCATAAAGAAA TAGAAAAGATAAGGAAATGCCTTAAATGTCTGGAGTAGACCTCTCCTTTG AGTACCTATAATGTACAGATTAGTGTTCAAACTCTGTCTGAGACATTTTT TTTTTGAGTTCGGTCTTGAATGATTTTTTTAGACTGATTTGGACCCATAT TTCACGGTCTAGACCACAGTTTGAAAGCGTACACCGAAATTAAAATCATA GAGCGAAATTCAAACGGTGAACCAAAATTTTATTTTCTATTGTACAATTT TTTACTCCTTTCTTAATGGCAAGAGTGTTTGGAATAGTGTTGTATCGACT CAAACAATTATCAACTTGGTTTGATTACACTTGAATTGAAGACTTTCAAT TCAACTCAAATAGTGAGTCAATTTACAATTTAAAAACAAATGAATCAATC AATTTCATATTTTAATTGAATTGACTTGATTTTTATCCCCGAACTGCAAC AAATGTGCTTAAGTGATTTTCTAATCAATAACAATTTTAATTATTTATAT GCCAAAATTCAAATTGTCTGGATTACCTGTTAATTTTGGTTTCTTTTTTT TTTAACATGCCCATTATACACACGATTTTCATCACTATTCATAACACTGA ATGAGGGGAGAGAAGCAAAAAATTAACAGCTGACAACAAAATACAGCATA ATTTTTTTTCGTTAGTGAGGATCCTCAACGACTCGACTCAAGTCGATACA ACGCTAGTCGAGAGTCTACCTCACCTTAAAGTACCTATAATGTACATATA AATGTATGTACTCGTCATGAGCTTTATGAGGAAAAAAGCAATAATTAATT CGTCAGACAATACTATAAATAAATAATAATACATAGTTATTTTGCTTTAT GTTAATGAGTAGTGGATTATTATTATTCGAATAAATATACACAATATGTT AATACAAACAAGAATATGATACGATGCGCAAAAATCCCCAAAGAATTGCT GTTCCTTTTTATTTGTTTAATGTTTGCTGTGTATATAGAAAAGTTATTTA ATTTAAGTCCCTTCCACAAGTTATATTATCTCCCTTTGTTTTACTGGTCT TATTAATGAAACATGTCCATATTAGTCAAATCGAATAAATTATCATTGTT ATGAAAGATGGGCAAGGAAAAAAGATTCAAAACATTATGTTTCTATAGAA TACGGCCGCATCTGTCTTCACATATTCAAGTTAGAATAATTTACATAAAA AAGATGTAGTAAAAAGACATCTATTATTTTTCCGATGTATGGCTTTAGTA ATGTGTATCCCACTTTTTATAAGTGTGCTCGATTTACACTGGATAGAGTT AGAAAGGTAAGATTTTGTGCTTAAAAAACTTATTGAATAGTTTTGTGATT GGTTGATCTAGTATTTCTTGAGCCTGCGTCAAAGATGGGCACCAAAAAAT AGAAAATATACCATATTATATGGCTTTTTTTTTTCTTCTATAAAAGCAAA AACGCAACCCAAGCGGCTGTGAAAAGTGTTTATGTTCTTGATACTATAAC AACCAATTATTCCAATTTTGGCTACGTCGATTCCATTCTAATGATTTTGA TTTCAAGGATGAAGTACACAGTGGAAGGCCAATTCTCGGAAATGTGGATA AAATAAGGGAAACTGTAGTGTCCAACCGGCATGAAAGCACTTTTTGATTG CCAATGAGATAAACATTAATGGGAAAAAATAAATCAATCAGAAAAATCTA TTAAAAATAATGGATTAATATAAGAATAAAAGCTTCTATAGCAACATTTT CCACAAAGTTTCAAATCTTGATGAAGAATTGATATGTAGCTTGTCCATAC AAAAGCAAGGCACAAGAATTTTTGAACCAATTGAGCCTGAACTACATATT TTGTAATCTTTGACAAATTATTATCAATTTTTACTTACAAGTTAGTCTGA TTAGCTTTTTGGAGGCGCCGCAAAAAAAAATAATTTAAGTTACAAAAGTT TAGTGTCACAATAAAATAATTAGAAAGGGGTATATAGGTGCCCAATTATG TAAGTCTCATAAAACAAATAAAGATTCTAAGCTATAAACTTTAATTGAAG AAGTTATTTGGTTAAAGATTAATTCAAGAGTAAGAACATTCTTTTTAAAA TTTAGCTTCAAATTACTCGCGGCTATCTAAATACCCGGTTCTACCTGAGA CCAAATTTCAATAATCCGAGACTAATCGGTTCCAAAAAAACAACCATGCA GGACCCATCCCTAGAACAAATATTTGATGTAGAGAAAACAATTGGAAATA TTCCCTATACTTAATTTGTGCAAAAGATTGTTTTTAATTAGAGAGGTTTT GTGCACCTAACAATCTTATAAATCAAACATGACTGCATAGTAATATATAC ATATTCCTGTTGAAAGGATGATGTGAGTACATATATAAGTCTCTGAGATC TTCATGTGGCATATTTTGACTCATTCATTTCAAGAAAAAAAAAAACTAAT GATGGAATCGTGGAAGATTCTTATCAAATAACTGCTGCTGTTTCCAATTT TTAATATCCACGTACCTACTTTTAAAATAAAATGTTTGAATGATAAAGAA GTCTTAGATATCAAATAATGAATTTGGCAAACTCCAATGATTGAATTTCA GATCTTTTCTCTGTTCCTTTTAGATGTGGTTTTTTTTATAGATATTGCCT ATAAAAAATAATGAAATGATTATTCAAAAAATTAAATTTTTTGATTTACT GGGTTGGGATCGGAGTAATCACATAATATGTGTAATCTGTATAATACATA ATAAATAACCATCCCAGTCCAATTATATATTTTATTAAAAAACGAGGKCC GCTACTCCCCCTGTGGTGCCTTACAATACTAATAATTAGTGTTGGATTCC AGTTATAACTACCGAGTTCGAGTTTAAACATCAGACAAAGAAAACTATTT TTATATTAAATTGATGAAATCTAAAAATAAATACAATATTTACGTCAGTT TGACTCAACATTACTTGCCTAATACACAAATTTAATCTATTTTATATTGA CACATGTCCATGTTTCCATTTTTTTTCTCTTAATCTTCCAAATCCCTTAA GGTCTGAACAATTCCTAACATTTATTGTGTAATAATAACTCCATAGTTCA GAAAAATTGGTTCAGTGCTACCAATTGCTTTTGGCCCTTTTATTATTTCG GTTGAGCTCAAGTAATTTTCTGAAAATTTCCAACTTTAGCAGAGGCCCCA CTATACAGGGCAGGAGAGTCATGACATCCCTCTGATTGAGATGATAATCA AATTGCAGCTGAAATAATCTTCTTTTTTTTCTACTGTCCTTCATTTCAAA AACTTCAGTTTTTTCAAGTTCGAATAATGCTCACCTAACACTACAAATGA TACATTCATAATAAAAAAGAAATAGAAAGTTCAGTGAATAGTCATAATAA AACACATCAAGGGAACCAGCTGTTGGGTAGTTTTTCTTTTAATTTAATTT ATTTAATCATTTTGACAAATTTTATTACCGATGGTTGATATATTAAGGTC AAAGTATTTTGTTTAGAAGGAACGACTTCTCGTGCCATAAATCGTTGTTA AGAGTGAAGAGAGAAGATGGAATAGGTGGGTGTAGATAGATTACATCCAT GGGGAGGAAGCAGCATTGTTTAAAATAAAATAAAAAAGTTCCCTAGGATT TGAAGAGGAGGAATGTGTAACAAAAAAATAAATAAACAAATCAGTCATGA GATGATCTTTAGTGGTTCATTTGTTCTTTATTACATAAATACTAGTAGGG GTGGGTTGGTAGAGTATCGAACTGGGATTCGGTCTGGTTCGACAATCTCT GTAAGATAAGGTAAACTGTTTAATACAGTGCCCATTGCAATTAATTATAA TTTTCTTTGGGGAGTGTGTTGCAATTTTTGACCTAGCTTTGAGTGTATTT TTAAATTACTTTTTTTTTACTAATACAAAAWATTTTATAGGGAAAAACAG ATGAATTTTTTTCACTATATATAANNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNGGGTGTAACACCCCCTTCTCCTGTATTCACAGGCCTG ACTAGTAGTTGTAGTCATAATTTAATTATTTCGTGGGGGGGTGGGGCTGA TTGRAGTGACGATTGTACCACTAATAGGGCCGACAAATACCCTTTGATAA TTATGGCCCAGTATTAAAATATATGTACCAATTGAAATAATATTTTCTAC AAGGTTGTGGCAGTAACGTAACTACCTAGATACTTGTACTTGACAATCTT ATATTTCTAAATTTGCCCTAGACATGTGGCAAAAGAGAGGTTTAATCTTA GCATACTTAAATGTTATATAAATTGTATTTTCTAAAAAAGTCATAATAAT TTGTTTTCAAAATTTGAACTTTTAAAAGAAAGTTTGTCAAAATTATCTCC TGCACTTCTCACTAGGATTTGAAGTGTCCCTCCGTGTACATAGGTCTAGT TATAAGAGTGTCTGGCGATATGTAATAGTAAGTAGATCATGTCTATAGCC AAAAATTGTAGATACGCTAATCTTTTTACTTTAAAAAAATCGAATTTTGA ATAGTCAAAACGTTTAGATTACGGACTTCACAAATAGTTAGATTTATGGG GCTTTTTTTATGTTGGTATGTAAATAATATTTTAAAAGGTTTTATTGAAG AAGAAAAAAAAACAAAATACATAAGTATTGCTAATAATTAGTACGATGTA GCGTACAATGAACCAAATCTTGGGTAATATCACGGATGTTACGCACCCTT CTAGCCCTAATAAAGACACGATCAAAATTCTACTTACTCTTTGATATGTA TAGTTATTTCAAAACAAAAATATTTCAAAAGAAGGATTTTGGATGTTCTC TGGTTCACCCATTGAAAAAAGAGATCTTGTCAATGATAAATCCTTCATTT ATGGTTAAGATCTGATGGTTTTTTTTAAGAAAAAAAAAAAGAGACAAAAT TTTTTTAACGCATGTAAAACACGTGTAGATTTTGATGTTTTGTTTTTTGT CTTTTTTGAAAGGTAACAAATATTTTGACAGTTTGAAAATAGTATTTGTA TATATATACATAAAAAGAAATACTCATCGTCATAAATGTCAGAACGGTTA TTTATGTTAATGATTTTTGGTACAATTTCTAGGATTGCCTTTAAAAAAAA TAAAAGGTTTTGTGACTTATTTTATTTCAAAAATCACCCAACCTTGACAT ATTTATACATTTTAAAAAATACCACACAATATTATAGTCCTTGGGTATCG CCTACATGAAATGTCTCAAAGATTGAAGTTTTTTTAGTATTTAATTTATA AGTAAAACATATTGTTCAACTTGTTTGTTTTAGTCCAAGGGTTTTTGGAT GTGTTTTATTATTTGCAGTTTCTCTGTTCTATCAAAGATAGTGTGTTTAT TTTTTGTTAACACCTATCACTTTAAGAAGGATTATTTTTTGTTAGGCTTC GTTTGAATAAACAAGGAAACTAAAAATTATCGAGTACATCGTATAGCTAT TAAATTATTGCAAGCATTGTGCAGAATACTTTGTACTGAAAAACTAAATA GAAAAATATGTAGAGAGTAACTTTTGACTCATATCCTCATCATATTTGAA GCAGTAAAGGAGGCCTAAAAATGATTCAGTTCGACAGTAACCTCCCATTT TTTTCAAAATAACATGGTCTAAAAGGCCTTAAACTTTCCGAAAGAAGATT TTTTTATGTCTTCAGCAGCTAAGAGCTTTGTGGACTATGGACATGGATAT TAACCTGCAAAGGTTAATGTATACCCATAACAAGGTATAAAGTAATTTGA GGATGTGTCAAAGTGATTTATGCGTGAAAAGAATAATATGTTCGTTTTTT CTTAATTACCTCTAAACGCATATTTCGTGGGGATATTGCAAAAGTGACGT AACTGACCTTTTTCTCAATGTAAGATATTTTCATTAGAAGTTATTTTATG TAATATATAGAATCATTATGATGATTGATGTTTTGTTTTTTCACCGTAAG TCAGGATACTCAACAACAGTCAATGAATAATTCAGTCAAAAGGGTGAATT CACTGAGGCTGCATAGTTAAATATAAAGTAATATATTCTTTGTTTTGCCA ATTGGCTTCACGGCTTTACCTTGCCAACACAAAATACCATATGTATTTTG ATGTCAGCTGTCGATTCCCTCCTCTCGCTTGATACACCATGGCTAATTCA TAATGAATTCATAATGAAAATAAAATAAGTTATTCTATATATACTTATGC TCTGTTTCCAAAAGAACAGGAATACAATTTCTTGTTGATCCCTCGTTGTT TATATAATAATATATTGCCCATTTTATTACATATATGAATAAATTTTGGC TATCATATAGAAATACAAATTTATGGGTACGCTCTTAATAGAATATTACT ATGTAAGCAATATTATGACACAGTATCGAATAAAATACTATGTAGATTGT AATACTATGAAATATATATATGTAAATCATTTTAAAAATGGTGAAGAAAT GATATGACGGTGACTGTCTTAGAGTCCATAAGATATATATGGCAGTTGGT GTTTTTGACTTATTTGGCTAACTACAAGATGATTTTTGGCATTTTTTTCT TTCGGAGGAAAGATTGCGTCATACATTAAAGGCAACGACGTGTTATTGCT TTAACTAGTACGAGTTGAAAATAGCAATTTGTTTAGGGTATGGTTTACAA TTTTGCTAGGACGTCATATTTTATCTTTCAAACTCGAAATTGGAAACAGC AAAAGAGGAAAGTTGAACTTTAATAATTGCAACATTTTCACAATATATAA ATAACTTCACTAAGTGAAGCAGATTATAGAATAGCTAATTATTGCCTATT GGGGATTGAGAAGAGATGTATTTGCACATACTGACTCTTGTGCGAGGTAT ATCTAAAAAAATAATCATATTGTAAAAGGTTCATTATAGATACAAACAAG AGCCGTCAGTTCATGGATCGACTCCGAGTCCCTTCCCCTGATACTCCATA TTAGACGAACTCACGGCTACATCCATCTATTTTTAAGATCCTCCAAATTG TCCAAATAATCCTCTCCTCCTACTCCAATAAGGATATATAATTCGGGGCC CTCCCTAATTATAATGAGAAGGTTGCGATAATTGAATTTCAACTTTCCGC TGTCGCTGACCCCAATTTCGAGATTAGAAATAAAAGAAGTCCAGGCAAAA TTGTAAAAAATAGCCATTGTCTTGTACAAGCTACATTTGTCCAACTTTAT CTTTGCAGGCTTAAAAAGAGCCGCATTTTGGCATCTCTAGTAATAATTAA TATTCATTTCCTGGAGCCCAAATGCTAATTCGTTCCTCCTTCTTCTACAG TGGATAGGTAGTTAGAATCAGAAAACCTATCTCGGCATGATTTTTGCATA TGATTAAAATTATAATAATAATAAATGATATTATTATTTATTTTTTCTTC TCAGTAGAACCCATAAAACCATCCCGATATTATTTATTTAGATTTCTATT CTTCCAAACAGATGGTCTTGGAAGATGTTTGATTCTATGAGTATGAATTG AGAAGCTCCAAGGGTGGTGGCKTTACTCTTACTTAGAAGCCTGCCTCTTC TTCTTTTTACTCAAACAGGGGGGGAAAATAATATTTTTTAAAGAAAATTC TTACCCCTATGACGTCTTTGAATCCAATTAAACTTCTTTAAATATTATGA TTATCACTATTAGCAGTAAGCTTATAATAATAATAATATTATGTGGATTT GCAGCAAAATATTCAAGTTACACTATACATAAATCTGTTGGACTCCGAAA ATATATAAAAAAATTATAACAATCAGTGGTTTTGTTTATTCACTGGATTA ATTTCCTAGTTAAGAATCTACATATTTAAGGAATTTAAATCTTTTAATGA CTAGGGGATGGTTGTTAGACTCCTGATGAATAGCAAAGACGCTTTATACA TAATATTTGTTTAATTTCTTATGTGAGACTGTTCAGTTCTTTATACACAA TGGTATGTAGTAGATTCCTATCAGTTGGACCCCTTTTAAGCCACTTTATT TATGTTTTTTGATGAAAACAAATCAGTCAAGATGTATTATATATAAAATT AATATTCGATGTCTATACATGGAATGCGCACGCCTTTTATACCTTTTTAT TTTTTGACCAAGTAAAGGCTATTTTTCTTATCTTCCAAGGGAGTCATTCC AAGTGAAATCAATCAAAAAAGGACATTTTTCACAGCACGATCTTTGTTTG TTTTTTTTAAACTTAGCACATATTTTTAATATTAGTTAAAAGCTCAAAAA TTCCAGCCTTTTTTGTTCTCCGGTTCAGGATTTAGAGGCTTCTAAAGTTT CCCCTCGACATCACCTACCCTATTTTAAATCAATATATTTTGNAATATTT TTGAAGATATCGATCTATTTTAAAACACCATATAATAGATAATTGTTTTA CTCTTTTTTTGTATTTTATTCTATACACCATCAAAATGAGTTTTTGCCTA TTTGGTCACGAGAAAAAAAAATGAAACATGCCATTTTCAATTTCACTCAA ATTATTATATGTTAACTTGTAATATATATTAAACATATCCAATGAGTTTT TAGAGATGCATTCGATTCTACTTAAATGCTACAGATGAAAATGTATGCAT AGGTAAAGCAAATTAAAAGAACTTACATTAATTAATAATTATTTAAAGTT GTGTTTTGTCAATAATAAAATAATCTAGAATGAGTTTTCTTAAAATTGAG TTTGGATTAGTGTTGTGTGTAGGTCCTTATTCACGACTGCAATCCTGTCC AGTCAGATCCCATACAATACATACAATCCTACTTGTTGGTCCTTAAAAAA GGTAAAATAAATCCCTAGTGATGTTATTGAGAGTGGTTTCCTTGTTTTAA TTATTATATTAAAGAATAAATTATAGAAATAAGTAAAAATATTTTATGTT CGAATTTAATAGCATTATAATTAAAACATGAATATTTTTGCAAGATATGT ACAATACTTTTAATAAATATTGCATATATCTTATTTGGCTAATTAAGCCA TCGATATTTTGAAAAAAATCCAATGGATGATAGTTGTTGCGTACCGGTCT TCAGACTGTACGGAACAAATAAGCCCCGACACAACACTAGTCTGTATTAG ATATTCTGAATCTGTTGATTATTATCAATTTCTATTAACAAAATATTCTG ATGTGCCATTTGGAAGTAGCCTTAATTGCTTATCGCTATGAAATTATTCG AAATAGATAAATTTTGTTTGAATGCCCATATTGGATCGAAATTAAATCTT ATAAATTAAATGGAGGTTCTTAACTATAGATAACATCCTTGGGTACCTCA GCTCATACTGAAATTTAAATACAACTGTTATAACTGACCTAAATATGTCT ATCAAATATTGTGGTGTATGTATGCTGTTCTAAACAAATGAATTGAACAT TTTTTTCCTTTAATGGATTTATATTCAAGATTTTAGTTATGGTAACTCAC TGCAAATGTATAATAGTATTTAAATTCCTTATGACATTCTTTACACAATT GTTTTGATTCAATTTTCAATTTTATTTCATAACTATTTTGCTATTTTTGT AAGGGAAAAAATGAGTTCGCAGTACATGTATTGTATATAGTATTCCCTGC TATGACCAAGGGATCCACAAGAGTAATTGAAATCTGCCTCATCCATCAAT TTTTTTCATTATATATTTTATGTGAGGAGTAAACATAAAATAAATTATAA ACGGAAAAGAAGAAAAATAGATTTTTATCAATTAATTAATTTTGTCTTTT TTTACAATATACATAAATATCAGTTTAGAACCCCAAATTAAAGAATTTTT TACTTTAGAGCAAGGAAATTTTTTTAACATTGTAAGAAAAAAGTGAAAAG AATTAGTTATTCAAAAAATGTACATAAAGATTGTTATTTGAAGAGTAAAA CTCAAAAATTTAATTTCAAATAGTGAATTTTTTGGGAAAAATAATTCCAA AAATCTACAAAAACATTCACAAGAAAATAAAAAAATTCCAAAAAAATATT AAATATTAAATTTTTTGGAAAGAAATTTCATAAATCTATAGCAATTCACA AAAAAAAAATCTAAAATCTATAACTGTTATAAAAAAATTGAATTTAAAAA AAAAAATTTCAAATATTATTTTTTTGGGGAAAAATTTCAAAAATTTATAG CGATTTACAAAAAGGGAAATTTTTGAAAAAAAAAATTGAAAAATGAAATT TCAAATATAAAATTTTTTGGAATAAATTACAAAAAACCATATTTATTCAC AGAAAATTACTATTTCTTCAAAAAATTTCCAAAATGAAATTTCAAATATT TAATTTTTTTGTAAAAGAAATAAAAATCCATAGTATGTTATATAATGTAG AAAAATAAATTGGGCATTTTTCCTTTTCTTTCCCTTACTTTTTGTTCAAG AGCTTTTTTTTGTATAAGCTCCAGTATATGTATTTCATGAATTGATCTTC ATGTACTTTGCTTTGATATATTTTTTAATTAAATCCGTAAAGACGTCTTT TTCCTCCTCTTTGTCCTTAGTAGTAGGAGAAAATACATTATATATTTATG TATAGTTCAAAACGACTATAATTAGTTGGTAAAATTGTAATTCGATATTT TCAAAAAGAAAGAAAAAGGCGAGAGAAGTTTCTTCGTCATTAGATCGAGT ACGGGTAGTTCTAGTACTGAATTGTACAATAATCATATATACCGCTTTTG AAGTAGAGAACATCATCTACATATTATGTACATGGATTGTAGAGTCAAAA ACACATATGTTGGAACCCTTAATAAAAGGACAAAGTCGTCGTCTCCTTAT AATCAGATATATATACATAATAAATAATGATCTCCCAATTACAGGAGTTC GTTCCATAGTATTTCCCCTTATTTATAAAGATTAAATAACTACTATTTTT ATATGTCAAATAATGTTTAAAATATATAATGTAATGATTATGTCATTTCC TAAACATTATTAAATATATTTCAACCTCTTTGACAAATAGAAATATAAAC ACACTTTTATCATTCTATACTACGAATTATATTTGAGACGCAGTGACGGC CATAGGGCCCCCCCCCACAAAAAACCCCGTAAAAAAGTGAATAAAATATC ATTATGAAGCGATAACTCAACGTTTTGATTGACAATTCATTTAACTAGTG ACAAATTCATTATAGGGTCTCTAGATAAGTATATCTTCACTGAAAAGGGC AATCAAGTAGTAAACCTGGATTTGTTTTCATATACATTTAAAAAGAAGAT ATCAAATCAAGATAAAGGATTTGTGAGGCTTGAATCTTCCCGATTAATTA TGGAGGNGAGGCATCGTTTTTCCTCCAAAACCAAAAATATGATGAATTCA GGCACTTTCACCAAAGCATTCAATTTTCTTAGACAGGAATGTGATTTTAC TCATGGTAAAGGCTTATTAAATCAATAAAGACCATTTGTCGGTGAAATTT AATAATGTGCGACGGCTAATAGAAAAAAAAGCCCTTCAAGGACAATGCAT TCACAGTTTGAAAGATATTTTCCATTTCCTTTGGCATTTCTCTAACCCTT CTTGTAACATCAGAAAGCTGTGAAAGAAGTTTGTAATGCCTTAAGAGACT AAAAAACTATTTGAGAAATAACAGTGGGGATAATCGTACAAGTGATTTAG CTATACTTTCAATAAATGTATAGAGAATGACGAAATTAGAGACGTTTTTG CTCTAAATCACAATAATATAGGAATTTCATTTGTGTAAATAAATATTTAC TTTAAAAAGATAATTAAATAAATTTGTTTGATGTTGTTCTATACATATTA AAGCAATATCATCTTGAAATATATAAACTTTAAACGTTTATATATTTTCT CATGGTTGTTTTACGTGACACATATTAAATGTTGATTAGCAAAACTATCA CTTATAGTAAGCATCCCCATAGTATTCCATAGCAACCCCTATCAACGCCA CTGTTAAGATGTATCATATATTTTTTTTGTTATAAAAAGAGGAACATGGA TTTTAGCAATGCACAAGTTGACTATTAGCCCACGGGCGTGCTGTATATTT TGCAATCATACAAACCTCAGCTCCACAAAAAAACTAAATCGTTCAGAACT TACTATTTTAGTTGCAGTTCCAATTGGGTAACGGAATTCAAAAATAGTGG AACGCACGGTCTCAAGGACTAATGAGTGAGGCTAAAGAGACTTTGAGGAA ATAATTGACAAGACTGTTGACACGTCCTTACTTTCCACCGAAAATAAATT ATGATTCAATAAGTGTGGGGAAACTATTCAAAGCTTAACAAAAGTTAATT CTGATTCAAGGAATCAACGTTTTGAGACCGTGATTAGGAGAAAGCAAGCA GAAAAATCAACATCTGACAAGAAACTACTGTCAGTCGTCTGACTTAATTT CAATAGATGGTACTAATGTTGTAATTAAATAAAGCTTCATTTTAATTAAT ATATATATTCTGCACAAGTTGTAGTTTCTAACTCTAATTTAACTTTTTTT AATTATAACATGCATACCAACTATTAATTTATACCATCCTTTCATATTTA AACGATTGTAATTAAGTATATGTATTTACTAGTAAATAAGTAGTCAGTAT TTGACCTTTGTAATGCCCCATAAAATACTTGTATCTAATCAGTAATTTTC AACAAAATAGTCAAAAATGACACCATTAATATATATATATATCATCTAAG GAATAATCCCATTTCTACATTATTTTTGTCACAAATATTGTAAATTCTTC ATATATAATGCTGATTCTCACTTTTTGGTAGCAACTTGTACACACCATAT CTATAATATATAATGATCTCCCAATTACGGTAGTACATAAGTTTCATATT ATTATTAATACATGATTATAAATAACAATTTTTTAATGTCAAATACAGTT TAAAATGTATATTTTAACGATTTGTGTTTGATTATAATACTACGAACAAG CACATTTAGATCATACATGTAATTAATTATAATCATTTGTAAAATCATAA ACTGGGGTAGAAATCCCCCACTGATTACAATCAAATGGGATAATAATTTT AAAAAATTATAAAATGGGGTGTGCGTCTACATAATATTTTGTATGTACGA TGTATGACCAATGGTTTTTTATTTTATTTCTCTCCCTTATTTGGGCAGAT GGTTGTTAGGGTAGCACTCACAACATCATCAGTTGTTGTTATTCTTCGCT ACTGCTATGGTATATAGGTGAAGTTTCTTGTGCAGAAGTCTTTTAATCTG TCCTCCACCTATGAATGTTCATATCTCCAGTTGTATTTTTGGACAATATA CATATGAGGTGAATATTTGAGTTTCTGGCGTGGTCACACACAATATATAT ATATACGTTTATAAAATATCTCGAATTTCAATTCCAACCCCCTTTGAATA AGAGAAAAGAAAAGACATATATTCATGTCACACAAAACATAACAATTCAA TACATTGTAGTAACAACTCCCCTAGCTTATGCGTAGCTACCTAGATCGTA TTCCTGACCTCAGGAGAAATGAGTATGTTTAAATCATTCTCTGTCTTGTG TAGGTGTCCAAAAGATGGATTTCTGATAAGAGAGTAGGAGAATGAAGAAA TAAACGTCTTATTTATAAATATTAAGTTTGTTAATAATATCATCTGAGAA AGTAGGAACGTGACATGGATATTAAAATCACATCCATGACATTAAAATCC CTTATGAATGATATTGATGATGTAAAATATTATTATTCTGAGTAATTTTT TCATATTTTCTTTTCAGCATCAAGTGGTTCAAATGTAGAGGGATGTCGCC ATTCTAATTGTCGTTTTGGAGCCTCGTGTATAGTGGATGCCAAGAGTGGA GATTCTTATTGTGATTGTAAAGGGATTGATTGCTCTGGACGAATAAATAA ACCTGTTTGTGGCTCTGATCATATCACGTATAAGAATGAATGCGACCTGC TACAGGCAGGATGTACAAAAAGAATCAATTTAACTGTTATCTCGGATTTG CCTTGTGAGGAAGCCCATCTTTGTGGTTCTAAGGGATGTCCTTTTGGAGG AAGTTGTGTTCGGACTGGAGGTTTTTATCGCTGTCTTTGTACGCACAACT GCGATGATAAAGAAAATAAAAAAATTTGTGGTAAAGAGACCGATTTCAAA CATTGATTTATCAGGGAGCGCACAAGAGGGGGATAAGGGGCCCAAAGCTA CCTCACTTTTTAAGAATTTACAATTATCATGCAAAAAAAGATCTTTGTTT TTAATTTAAGGCCCCTCCAATTTCAAGAATGGTGCGCGGAGCCTGTTTAT CTATTAATGATTAAGAAAAACCCACTTTGTTAATAATTTTTTGAAAACTT TTTTCTATTACTCCAATTAGGAAACAATAATAAAATTTACAAGAGTCAAT GTCATATGGAGTTGGATGCCTGTGAATCTCGGCTAACAAACTTGACCATG AGTTCCCATGATAACTGCTGTAAGATCAAGTTTTATTTCTATTCTACTTT TTTAAACTATCTTTTTGTCCTTCACTTTACCATTTTGATTCATTATTATT TTTTTTTCTTTTTTACTTCCATCCTCCCACGCTCTTTAGACTCATGCAGT CAAACATGTAAATGGAATGAGGATTGTCTCTACTCAGAGAAAAGTGGTAA AAGTCTTTGCAGTTGTCCCGAGTGCCCCTCCGCTGGGAATGAAGAGGAAG ATCCAGTTTGTGGAACAGACAATATTACATATGACTCTGTTTGTTCTCTG AGAGCAAGCACTTGTAGCAAGAAACGAGGAAATGACGTACGAGTTAAATA CCCTGGGGTCTGTCGAACCATCAAATATGAGAAACCAATTCCTTGTGGAA AGAGTTCCTGTGATTTCGGGGCAGTGTGTGAGGAGGACAGTTATTGTGTC TGCTCTGTTACTTGTAAGGAGGAAGAGCAATTCCCTAAATACTACGCTGG AGCCGTTTGTGGAAGMGATGGACGAACCTACGAAACGGAGMGTGATCTAA GACGTCATGCTTGCAAGATGCAGAAGGAAATTGTGGTGGCCGATTTCAAA CCTTGTACACGAGGAGCTACAAGGATTGGAGATTACTGTCTGCATGAAGA GTCTGATTGCTTGGCCCTAAACAGTGAATGCGTAAATAATCGGTGTATTT GTCCAGAGGGAACGAGCCCGAATAGTGCGAATACATCTTGTGAAATAATG AAGATAGGCTCACTCTGCGACCCCAATCCCTGTCAAGGAGGGGGCACATG TGATGAACATGATGGAACATTTTCATGCTATTGTCGTTCAGGATTGACAG GCTCACGATGCGAACATGACTTATCACTGACAGATATGAACACAGCCTCC TTCACGGGTCAAGACTCGCATGTAGAAATTAAAATACCAACTGACTCAGT TAAAAGAATGGAAATTGAAATCATGTTTAGAACGTTTAACGTACAAGAGG GGCTATTACTCTATGGACAAAGTGAAAATGAAAATGACTTTATTTCCCTT GGGATTATGGATGGGMAATTATTTTTTCTCGGAACTTCTCTTTAATTAGG CGTTTGTTAGTACGTAGGTTGAGAGATTGAATCTAAATAGTTGCGTTCAT TAAATGACATAATAATTCATACATACATACATACCATTAAGGTGTTAACG TTTCAAATTATGAAAATACGATCAGAACATTTCCTTTCTTTATACCTCTC TCTTCTTCGAATAATAAATGTCACGTATTTAAGAACATAAAGTAACGCTA TATTAATTACATTTAATTGATGTGCAATTAATATTAATTTAATGCAGTTT TGCCTAATTTATGTTCATTATTTTAGGCATATCATATTCAGCTATGATTT AGGAAGTGGCGCCGTTACTTTAAAATCCCATGGGAAAATTGAAAAGGGCA AATGGACACATTTGGTTGCAAAGCGCTATCATCAAGATGGTTTATTAGAA TTAGAAAGAACAGACAAGGTCATAGGTTCCACAAGGGGCTCTCTTCGTAC TCTAAACCTTCAGGGACATTTGTGGATCGGGGGCGTGGATCCTAATGATT TTCCACTTATGTACAAAGTTCGTAAAAATGCTGGTACTTCCATTGGATTT GTGGGTTGTATCAAGGAGCTCAAGATAAACAAGAAGACTATAAGCCTTCA ATCCATGAGAGAGCCTTTGGTGATCCGGAGACGGGGTTTGGTGGAGTGTG AAGAGAATTCTTGCAGTGGGAATCCCTGCAAAAATGAAGGACTCTGTATA TCTCAGGTGCAAGGCTTTCTCTGCCATTGCAAAAGAGACTTTACAGGTAA GGAGGGATGATGAAGTAAAGGCTTCTAATGAGACTCTTATATTTAGGAAA GAGCTGTCAAAGACGACGGAATGATCAGTGCGATCCAAATCCCTGTCAAA ATAATGGAGTTTGTCATATAAAAGATCGTGGATTCGTCTGTGTTTGCTCT GGATCTGAATACTATGGAACTTATTGCGATCTTAAAACTGCGGATACTCC TAATGATCAACCTGCATATGGGTCTGCAAAGGCTCCTAAGTATGTTTTTT ATGCTGTATACTTGAGTGACCAATCGGGTAATTTTTTGTCCGGACATCTC ATCTTTTTAAATAATCTCCTAAATACTCGAATCCTTCTTTTAAAATTCAA AATATGTTGAGATTTATTGTCATTGTGTACAAATGAGAATAATACTGTTA CTTGTGACGGAATTTTAGTAGAAAGCTCCAATAAAAAAACGATCCCAAAT ATAGTTTCTGAAATATATTATAACAATTCGTGCCTGATCTGTCTAAATTG CTTAAAAATACGGTATTTTATAAAACCGACCGTTTGGACAAAGAAGGCCA GATTTAGCAAGTGGAAATGTAATTTACTGCCCAAGACGCGCACAAGCGCC CAATTTTCCTCCCCTAAATAATACAGTAGGCATATTTATCATCCAGGGCG TAAAACAGACCAGAATCACACAACTGTATACTGTATTAGCCAATATTTAA TTAACTTTTTTTTAGGACTCATTTCAACAACACCCTTGCGTTTGATCTTA AAAAGAGTCTGGGACTCACTGAAGAGGCTCTAATGGAATTAGAAGTATCA TTTTTGAAGAAAGACCCCGGCTCCCTCGGTCGTTTGAAAATATCTTCTAC AAAGACAGGTCTTTTAAAACTGCAATTATCCGACTCTCAAGGAGCCTTGA CGGAGTGGATTACGTCACCTCCCTTCTTTGTCTCGGATAATCGTCCTCAT AATATCATACTTAATATATACCGTGGAGAATTGGATTTGGATGTGGATGG GYTGYGTGCATTCACAGCTGTGAATATAGATGAANCTTTTCATATCCTTG GGTTCAAAGGTAAAGTAGATTTAAACATTTGATTATCATCAGTGTGGATG TAAGTCCATTTATTACGCTTCTTTATTTTATTTTTTTCATATAAAATTGA TTGGAATATAAAAGGACTTGCAAGGAGACAGAATGGATGTTTAACCAACA TTCGTTTATCCCTCTCAAGTGACTCCACGTCGTCCATGAATGAGAGGGAG GAAATTGTGGAGCCCCCCGACTCTTGTTAAAGGAATGATATGAAAATGAT TTCACCCCATAGAGAGAGAAAATATATAGTGACAACAAATGGATTTCGAC TGACAGTTCGGCATTCTAAGGCTATGGTAGATTAAAATCTTCTATTAGCA GCAATTAACATCAGCTAAGACAAAGGATAAAAACACATATATTCTAATAC CTTGAGATACGAGTGTCACAACATGTTCGAGAGTTTAAGGCAACAGATTA CGAGAGCCACTTGAAACTATTTTTTTATTTGCTAGTTATTTTTTTTTTTG GCAATAAATTATTTGAATTGCGGGCTCTGTACATGAACAATGAAAAACTC GTACCTCGAGGTGTTACTCTACGGCATTTTATCCTAGGGTTTAAAGGTGT ATTTTAACAAAGGAACCCCAGTTGATTGGTTAGATTCTTATAATACACCA ACAGAGCCAATCAGAATTGAATATTATTATACTATTGGATTGCCTAACAA TAAGAAAGACAAACAACTGTGACGTCACATTGTACTCATTGAGTCTGATA ATATCCTTGCTCAATCATTCGCCCATCCTGATGCTCTATATATCGTATTC TTTCTCTATGCTAACCCCATGCTCATCATCTGTGATATTATCAACTATAT AAATCTAAAAGGCTGTGCTTTTAATCAATCTTTGTTTCTTTTCATTTTCA AACAATATATATTTATGTATGTATGCA
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