EMLSAG00000004534, EMLSAG00000004534-687300 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000004534
Unique NameEMLSAG00000004534-687300
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:sns "sticks and stones" species:7227 "Drosophila melanogaster" [GO:0007155 "cell adhesion" evidence=ISS] [GO:0005886 "plasma membrane" evidence=ISS;IDA] [GO:0007520 "myoblast fusion" evidence=IMP;TAS] [GO:0007523 "larval visceral muscle development" evidence=IMP] [GO:0007157 "heterophilic cell-cell adhesion" evidence=IDA;IMP] [GO:0030036 "actin cytoskeleton organization" evidence=IMP] [GO:0036059 "nephrocyte diaphragm assembly" evidence=IMP] [GO:0005515 "protein binding" evidence=IPI] [GO:0005917 "nephrocyte diaphragm" evidence=IDA] [GO:0097206 "nephrocyte filtration" evidence=IMP] [GO:0007156 "homophilic cell adhesion" evidence=IGI] [GO:0061321 "garland nephrocyte differentiation" evidence=IMP] [GO:0001745 "compound eye morphogenesis" evidence=IMP] [GO:0007286 "spermatid development" evidence=IMP] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 EMBL:AE013599 GO:GO:0005886 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0007286 GO:GO:0030036 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007520 GO:GO:0007157 InterPro:IPR013098 Pfam:PF07679 GO:GO:0007156 InterPro:IPR013162 Pfam:PF08205 GO:GO:0001745 GO:GO:0007523 ChiTaRS:sns GeneTree:ENSGT00550000074545 GO:GO:0061321 UniGene:Dm.14097 GeneID:44097 KEGG:dme:Dmel_CG33141 CTD:44097 FlyBase:FBgn0024189 GenomeRNAi:44097 NextBio:836750 GO:GO:0005917 GO:GO:0036059 GO:GO:0097206 RefSeq:NP_001036532.1 ProteinModelPortal:Q0E9F2 SMR:Q0E9F2 IntAct:Q0E9F2 PRIDE:Q0E9F2 EnsemblMetazoa:FBtr0111043 UCSC:CG33141-RB OMA:EGYSAGE Bgee:Q0E9F2 Uniprot:Q0E9F2)

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 349/958 (36.43%), Postives = 516/958 (53.86%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET---------LSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMN-----------------------GSSVAEGG-----------------------STNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            GQTV L+C S GGNP A+++WY+   QI  +Y T+GR S N+YTF A + DN  R+RC+A N +S  PL A++ LSV FAP  VT+ GPTEA VGD +   C T  SNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I+G   G I+  G  ++L C+S  GNPLATLTWY+ D+ I    +  D++       +E+ + AN SDN   YRCEASN AT     PL  + TLSV F P T+ ++ +PE  + G +A + C++ SSNP A + W + DG+ IE       + G +GG ++T    ++V    +G  + C++ NE L  S ++ +++            D + +P F   P+ T   V EG  L V++  RANP  VTY+W           T+  D   RI++    LN T   ++DAG Y    +NS+G + L I + V YG TI  +++ ++V  GE A   C V+ KP+T + + W RVG+DM  +TS    NGT+ +H  + +  +ED G F C A+N + D    +   L+V+  P I ++P   +AAS   +   L CRAQG+P   F W +         ++ +       KYE++   ID +T+ES L +  V+  DYG+Y+C+A+NE G +   V L +TS+PD P  LN+ N T+++V L W PGFDGG K S+ +RYR    + Y+Y+D   +S +   +  L++ T Y FSV ++N+LG S +  +    ET          S L G   P         + G++L       ++++LNV +I C + +R   R+                        G S+A  G                       +TN   K+ATIEMYA SSYN+T++GETLSS+SEKS  Y+
Sbjct:  300 GQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKS-----VSAEITILANVSDNQAQYRCEASNSATEI---PLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAKLSWWK-DGIPIEGINNTS-KPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSL------------DVLYRPKFVPPPSSTAVGV-EGESLQVSLQTRANPTPVTYKWTK------DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLH--IKDAKREDVGNFRCVADNRV-DNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQ---------DKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNS-HKLTIGGLRMNTLYLFSVMSWNELGQSSYLPDLARAETKEAPPPSHPASSLGGG-PPTTSQTPLGGTSGMLLVGVGAGIVVVLLNVFVIGCCLHKRNEKRLKRGLELMPAELTEDSSNTPNLVIIGISLAAFGFLLVNASLVAWFFVHQRRKKVAETTNQPAKTATIEMYAPSSYNDTVTGETLSSVSEKSESYS 1214          

HSP 2 Score: 137.887 bits (346), Expect = 2.261e-31
Identity = 177/723 (24.48%), Postives = 276/723 (38.17%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLID-FIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGA-TISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT 705
             N    V W +    + FS        Y   G   + +Y    S  S +++  Y+C+         + A+  L+V   P  + I+G       E      L   CI  N+ P A + W    V+ +  +   TV  ES    + T S++ +  GP+D     +C A +  L   +    T+  SVLYPPG P I G   G  +  GQ+  L C S  GNP A L WY+    I+   +         Y       TA   DN   +RCEASN        PL + + LSV F P  + V    E  + G    L C T  SNP A + W    G  + N     + S P GG  TT+ +   VE     +  IC   N +L E+V    T+ V       PLI  +++  I        +  V  G           NP      + N  ++ I ++   +D      S+   + I     ++  +Y+ + +NS  +  L     + V +   T+    +   +  G  A   C   +    A  +SW + G  +EG     +  +  GT S      NV++E  G+ + C + N        ++  L V  RP+    P+++    +     V L  RA  TP V + W++ G+ I Q    D    I  +   + +  + R               D G Y C A N +G       LN+T
Sbjct:   96 ANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVE-ESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYT-----FTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAPTHVTVMGPTEA-RVGDIVPLTCTTAPSNPPAEIKWMVG-GRQVRNATSKTIVS-PEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPP-APPLISGYMEGQIIPAGSVQKLLCVSSG----------GNPLATLTWYKN--DKRINSVIRAADK-----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAK-LSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTP-VTYKWTKDGTTIPQ----DGDHRIFADGGSLNFTRLHR--------------DDAGIYSCSASNSQG----GATLNIT 764          

HSP 3 Score: 93.9745 bits (232), Expect = 6.357e-18
Identity = 179/833 (21.49%), Postives = 314/833 (37.70%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASS--------EDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDN--GMVYRCEASNPATAQESGPLSSTITLSVRFKPI--TIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMT-WESELHIKNVSSRDYGSYDCIAQNE-EGISRYAVYLNVTSRPD---TPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKG---SKTYQYVDVHHSSTNSFEVEDLKLGTKY------SFSVKAFNDLGDS 789
            Q + L C+     P A++VWYRG+ +          E      F  +S        +D+   Y C+A++       P+ A + LSV + P    IEG +       G T+   C +   NPPA + W  +   +Q     RT           NI         NK    C A N      +     +SVL+ P   ++ G     +   G    LTC +   NP A + W  G   ++  T     +    +  +  ++TA    N   +V  C   N    Q +  + ST T++V + P    I+   + ++  AG    L C +   NP A + W +ND         IR         +T     I     D+   + CEA+N      +    T++V           F  + +   I+P +    +E  + ++ + S    P  +++     + + IP   +++ + P ++     +++  +N+T+         +      +  +   + +DVLY         +  V V GE+        A P T  T  W++     +G T  Q+G + +      L    + ++DAG + C+A+N  +    T +  ++V     I+    N     +  + A+L+C  +G P  +    W R+G                   Y++            S LHIK+    D G++ C+A N  +  +   + L V   P+   TPTLL   + T     L       G  K  FI R  KK    ++TY+Y +V     +S   E   +  K       ++   A N+LG++
Sbjct:  194 QDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKN--GSQIRMAYRTSG-----RLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARV---GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLN---MQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK---RINSVIRAADKSVSAEIT-----ILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH----------FAPETVKIRIEPEELRPGME--ATIICDSSSSNPPAKLSW-----WKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANP-TPVTYKWTK-----DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASN--SQGGATLNITVVVEYGTTIKSVSEN--IVVNPGEDAMLSCTVEGKPLTEEHVKWERVG-------------------YDMTVKTSTTFANGTSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPC--RAQGSPKPQFIWRQDKKDLPINRTYKY-EVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA 955          

HSP 4 Score: 90.5077 bits (223), Expect = 6.264e-17
Identity = 130/611 (21.28%), Postives = 238/611 (38.95%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTF---VASSEDNNKRYR---CKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQS-ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTY-RWAN-PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS-EGKSRLKIKIDVLYGATISDITDAVMVV--TGETAHFECVVDAKPITADTISWSR----------VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT 586
            +G  V L C +   NP AE+ W  G  Q+  +   T       +T    + +  + NKR     C   N    E + +   ++V + P    I G  E  +   G      C+++  NP A + W  + +  + N  +R   +       + I++L   +D      C A N+     + Q+ T+SV + P    I  I+P   +  G    + C S + NP A L+W++    I+G               + E  +      NG VY C+++N A  + +      ++L V ++P  +   S   V   G+   +  +T  +NPT        DG +I   G+ R+ +   GG+L    L      RD    + C A+N + G ++N  IT+ V+  + +  + + I               V  G D +++ +V   P    + +W    Y+  +   T  ++         + L+I +A++ED G ++   +N  +  +   I + V +   I+     +     TGE     C     P       W +            +++E R  + + T    L V  V+  D G + C A N + +  +T
Sbjct:  393 VGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK--RINSVIRAADK----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIR-IEPEE-LRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSA---HEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQT-RANPTPVTYKWTKDGTTIPQDGDHRIFAD--GGSLNFTRL-----HRDDAGIYSCSASNSQGGATLN--ITVVVEYGTTIKSVSENI--------------VVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGT-------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSP--KPQFIWRQDKKDLPINRTYKYEVEER-KIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVET 958          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:hbs "hibris" species:7227 "Drosophila melanogaster" [GO:0005886 "plasma membrane" evidence=ISS] [GO:0061330 "Malpighian tubule stellate cell differentiation" evidence=IEP;IMP] [GO:0016202 "regulation of striated muscle tissue development" evidence=IGI;IMP] [GO:0007520 "myoblast fusion" evidence=IGI;TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0007157 "heterophilic cell-cell adhesion" evidence=IDA] [GO:0007156 "homophilic cell adhesion" evidence=IGI] [GO:0001745 "compound eye morphogenesis" evidence=IMP] [GO:0007220 "Notch receptor processing" evidence=IMP] [GO:0005768 "endosome" evidence=IDA] [GO:0042067 "establishment of ommatidial planar polarity" evidence=IMP] [GO:0010950 "positive regulation of endopeptidase activity" evidence=IMP] [GO:0016324 "apical plasma membrane" evidence=IDA] [GO:0001751 "compound eye photoreceptor cell differentiation" evidence=IMP] [GO:0070765 "gamma-secretase complex" evidence=IPI] [GO:0045747 "positive regulation of Notch signaling pathway" evidence=IMP] [GO:0005913 "cell-cell adherens junction" evidence=IDA] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 EMBL:AE013599 Gene3D:2.60.40.10 InterPro:IPR013783 InterPro:IPR003006 PROSITE:PS00290 GO:GO:0016324 GO:GO:0042067 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0045747 GO:GO:0005913 GO:GO:0007220 GO:GO:0007520 GO:GO:0007157 GO:GO:0010950 InterPro:IPR013098 Pfam:PF07679 GO:GO:0007156 GO:GO:0001751 InterPro:IPR013162 Pfam:PF08205 GO:GO:0016202 GeneTree:ENSGT00550000074545 eggNOG:NOG251936 OrthoDB:EOG7RBZ7J GO:GO:0061330 RefSeq:NP_788355.1 UniGene:Dm.1858 ProteinModelPortal:A1Z9X4 SMR:A1Z9X4 PaxDb:A1Z9X4 PRIDE:A1Z9X4 EnsemblMetazoa:FBtr0087414 GeneID:44129 KEGG:dme:Dmel_CG7449 UCSC:CG7449-RB CTD:44129 FlyBase:FBgn0029082 InParanoid:A1Z9X4 OMA:VHETISL GenomeRNAi:44129 NextBio:836838 Bgee:A1Z9X4 Uniprot:A1Z9X4)

HSP 1 Score: 471.085 bits (1211), Expect = 1.737e-143
Identity = 287/852 (33.69%), Postives = 444/852 (52.11%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRT--SMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVER-TISQGIILTITLVSGLLLILNVILISCYVKRRR 850
            GQ V + C S GGNP A++ WYR    I     T+GR S N+Y F A++EDN     C+AKN ++  PL A++ L+V +AP  V + G  +A VGD++   C+T  SNP A + W ++ R    +    T S  GGW + SNIS+ I    R  I  C+A+N +L + VV +HT++VLYPP  P +TG + G I+  G   +L C S  GNP  TL WY+ D+II   ++  D   T     SEL L  N SDN  +Y+C+  N A      PL +T TL V F P T+ +   P+    G +A L C++ SSNP A + W + DG+ +E    +  R G +GG+++T  + +++     G+ + C++ NE L  SV++ I++            D +  P F+  P  T F   EG+   V +    NP  +TY W     + +P I+ +S +  R+ S    LNI+   + DAG Y  +  NS+G + L+I++ V Y  TI+ +++    V GE A   C + A+P+ A  + WSR G+D+  RT  S +NGT    L + +V + D G F C  +N        ++  L+V+  P I+ SP  ++ A+     A L CR+  +P   F W R G  +  +   ++ + +E +        +D + +ES L I+N S  DYG Y+C+ +N  G +   +  +  SRPD P  L V N +   V L W PGFDGG +  F +R ++ G   Y+YVD      N   ++ LK G  Y FSV A N+ G S+F  +      L+  +G      +  E+  +   +I+ IT  + +LL+LN  L++ +V RR+
Sbjct:  266 GQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKIT-----SELSLLVNASDNNAIYKCKVQNAAI---DIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKISWWK-DGIPVEGL-NLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISL------------DILYPPKFET-PQSTTFVGVEGAPFHVELLASGNPMVITYTWT---KDGLP-ISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAHVRWSRDGYDLATRTISSFENGT--ALLQIASVERSDIGNFTCIVDNQ-RGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQ-RRNKFKSVERQ--------VDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQN-ISLDGLKPGATYYFSVMAANEAGGSKFMPD----IKLTLSKGSQPHSAEYTEKDELPNVMIIGITSAAMVLLVLNAALVAWFVIRRQ 1073          

HSP 2 Score: 119.783 bits (299), Expect = 6.090e-26
Identity = 171/731 (23.39%), Postives = 288/731 (39.40%), Query Frame = 0
Query:   15 NPLAEVVWYRGDEQIDFSYYTTG-----------RESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATIS------DITDAVM---VVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKE-DAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            N   +V W +    + FS    G           + + N+    A+ ED+   Y+C+         + A+  LS+  AP  + IEG       E      L+  CI  N+NP A + W    V   +A      +T  +R  + T S + +     D  K  SC A +  L   V     +  SVLYPPG P   G   G  +  GQ  ++ C S  GNP A LTWYR      G      +  +   +++    TA   DNG    CEA N      + PL + + L+V + P  + +    +  K G    L C T  SNP A + W  N G  ++N    +  S   GG ++++ + + ++++      +C A N EL ++V    T+ V        L  + D    DI  + +I  ++         S   NP      + N    N P+  +DS        I + L++     ++   YK KV N+       I I +    T+        +  +V+   +V G  A   C   +    A  ISW + G  +EG     R  +  G+ S      N++++ D   + C ++N +      ++  L +   P+  E+P ++     +     +   A G P V+ ++W++ G  IS       S  +  ++          ++    L+I  +S  D G Y C A N +G +   + + V   P
Sbjct:   64 NRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDD-AEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKR--FTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWYR-----NGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKN---LLATTPLRAELNLTVLYAPKDVYLSGANQA-KVGDSVQLSCVTAPSNPQARISWSIN-GRPLDN-STYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDG---DILISGSILKLQCS-------SAGGNPPPTLQWYKNDKIINAPSKLVDSK-------ITSELSLLVNASDNNAIYKCKVQNA------AIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAK-ISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILYPPKF-ETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPIS-------SNSLSGQRL---------ISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAP 739          

HSP 3 Score: 105.531 bits (262), Expect = 1.733e-21
Identity = 157/746 (21.05%), Postives = 296/746 (39.68%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFS-YYTTGRESRNMYTFVAS------SEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTH-TISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRF--KPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGF-----DMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKR-----KTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRM-TWESELHIKNVSSRDYGSYDCIAQNEEGI-SRYAVYLNVTSRPD 709
            Q ++L C++   NP AE+VW++G+  +  +   T  + +   +T  ++      +ED+ K + C+A++       P+ A + LSV + P     EG ++      G  +   C +   NPPA + W       +    + +     G  + +         D    + C A  N L  T ++    ++VLY P    ++G +   +   G S +L+C++   NP A ++W   G  +   T +    ++    + S + LT +      +  C A N    Q    +  + T++V +   P  +   +D ++  +G    L C +   NP   + W +ND +       I   S      +T+  L + V A D+   + C+  N  +   +    T+ V           F  + +   + P + +  +   + L+ + S    P  +++     + + IP   L+  N P ++     +++  +NIT+    D   Y  + +N   +  +   I +D+LY            V V G   H E +    P+   T +W++ G       + G+  + +G     L +  +S+ DAG ++C A N       ++ T LL  Q   +E +P  + A S+ R     + AVL C  Q  P       WSR G                   Y++    I       + L I +V   D G++ CI  N+ G  +   V L V + P+
Sbjct:  161 QNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWY------RNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEA-KNLLATTPLRAELNLTVLYAPKDVYLSGANQAKV---GDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQN---VVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKI-------INAPSKLVDSKITSE-LSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVH----------FPPETVKISVVPKNLVPGIR--AKLICDSSSSNPPAKISW-----WKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQ--DLDGIVYTCQSHNEVLQRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVI-TYTWTKDGLPISSNSLSGQRLISDGP---RLNISRLSRNDAGVYICEALN-------SQGTALLEIQV-AVEYAPTIT-AVSEGRSFVAGEPAVLACHIQARPLEAAHVRWSRDG-------------------YDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPE 834          

HSP 4 Score: 93.2041 bits (230), Expect = 1.097e-17
Identity = 127/612 (20.75%), Postives = 232/612 (37.91%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG-TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQ---GEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTY-RWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAV---MVVTGETAHFECVVDAKPITADTISWSRVGFDMEGR-----TSMQNGTNSMH----LTVYNVSKEDAGEFLCTANNGIADMKDT 586
            +G +V L CV+   NP A + W      +D S Y T      G  S +  +    S+       C A NT   + +     ++V + P    + G  + ++   G  L   C +   NPP  +QW  + +       +   S+    +  S +S+L+  +D N I  C   N  +   +  T T+ V +PP    I+ +    +   G   +L C S + NP A ++W++    ++G    +       + +  E+++      +G+VY C++ N    +    +  TI+L + + P     +S   V   G   ++     S NP         DGL I +    G+  +  GP         L I   +R+    +ICEA N + G ++     + ++      P I  + +            S   G   V+   ++A P +  + RW+           L +  I    +   LL I    + D G +   V+N  G    +  + V+  A   D +          G  A   C   A P    +  W R G D++ +      S++   ++++    L + N S +D G++ C   N +     T
Sbjct:  359 VGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDK------IINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLV--PGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRS---VHETISLDILYPPKFETPQSTTFVGVEGAPFHVEL-LASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPR--------LNISRLSRNDAGVYICEALNSQ-GTAL-----LEIQVAVEYAPTITAVSEG----------RSFVAGEPAVLACHIQARPLEAAHVRWSR------DGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASP--QPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTT 926          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:nphs1 "nephrosis 1, congenital, Finnish type (nephrin)" species:7955 "Danio rerio" [GO:0048741 "skeletal muscle fiber development" evidence=IMP] [GO:0003094 "glomerular filtration" evidence=IMP] [GO:0016020 "membrane" evidence=IEA] [GO:0001822 "kidney development" evidence=IMP] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 ZFIN:ZDB-GENE-051102-1 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0016020 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 GO:GO:0001822 GO:GO:0048741 InterPro:IPR013098 Pfam:PF07679 GO:GO:0003094 InterPro:IPR013162 Pfam:PF08205 CTD:4868 eggNOG:NOG251936 HOGENOM:HOG000113844 HOVERGEN:HBG031752 EMBL:DQ466904 RefSeq:NP_001035777.1 UniGene:Dr.67450 ProteinModelPortal:Q1KMS5 STRING:7955.ENSDARP00000080406 GeneID:692352 KEGG:dre:692352 NextBio:20902512 Uniprot:Q1KMS5)

HSP 1 Score: 354.369 bits (908), Expect = 2.083e-101
Identity = 269/949 (28.35%), Postives = 434/949 (45.73%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYT--TGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG---FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAAS--DKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSR-DYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSEN----VAVETLSYLEGMVKPEDKDVERTISQGII------LTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVA-------EGGSTNGSTKSATIEMYAGSSYNETM---SGETLSSISEKSGXYTSSS-VDPIHGHHHHIHHHPH 921
            G  + ++C+S+GGNPLA + W +  E +  S+      R + ++       EDN+   +C++ N V+  P++    L+V F P +VT+ G  EA  G+ ++  C T++SNPP  ++W +  +          E + GG  T SN++  +   D    + C A N     + V+   + V YPP K  +    P   +  G + RL C S  GNP   LTW + ++++   ++   +  ++     EL LT + SDN   YRC++SN A       LS+     V F  +++ + +  +  + G+   L C  GSSNPTAN+ W  + G  I    +   +   FGG   T+ L + +E         C+A +  L E VN+  ++            D +  P F  +  D +  +E+ +   +   V ANP ++T  W            L  +  PR       L I    + D G Y  + +N+EG +R K+++DV Y  T+   +D V V  G+TA   CV DA PIT+   SW  +G    D  G  S  +GT    LT+Y V+++ AG + CTA+NGI+     +   L+VR  P +++ P   K AS  D    A + C+AQG P V F W++ G  +          ++ + +YE        +   S L + NVS+  DY  + C A N  G     + L  T+ PD P+ L +      SV L W+PGFDGG  Q F +RY+  GS +Y YVDV   +   + V  L   T Y+FSV A N +G+S +   N    V        +G V   D+D    ++ G +      + +      LL   +  +    KRRR ++ N SS++       +  + + ST+ A+ E+   S+    +   S E  SS+ E  G   +     P   +   ++ HP 
Sbjct:  249 GSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVPFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASK---QVSSERGVSRELLLTLHPSDNLATYRCDSSNKAKKV----LSAQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISW--SLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLEPHHWRKRITCQAFSNVLSEGVNNFYSL------------DVLFPPEFSAEQPDEVQVIEDET-ATLPAKVSANPDEITCEWI------FQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGV--LTIYEVTRDRAGVYQCTADNGISPTGSVEGQ-LIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPL----------DLGNPRYEETTESTGTIH-TSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGESSYADNNKILTVTTADAKEADGGVSTADQD--SALAGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGNTSSISQETKSEEQRSTASSSTRYASRELINASAQRTLLVDSSSEPDSSVYESYGRQENPHYYYPTEAYSPALYAHPE 1153          

HSP 2 Score: 114.39 bits (285), Expect = 3.404e-24
Identity = 169/724 (23.34%), Postives = 276/724 (38.12%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYR-GDEQIDFSYYTTGRESRNM------YTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQ-SELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDF-IDKPIFDIDPTDTIFSVEEGSDLVVN-ISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNL-----LNIT----EARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATIS-DITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRP--RIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDE-SEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGIS 696
            G+  ++ C++    P AE+  +R G+E ++   YT       +       T  A S D+ +R  C+AKN  S   L   + + V F P    I G    E   G  L   C++   NP A + W  +      ++ V   S R      S + + + P D + ++ C +IN      +  T T++V + P + ++ G        EG+   L+C + + NP   + W+ G + +  T     E +       S L    +  DNG+  +CEA N  T   S   +  I   V + P  + + + P      +G    L C +   NPT  + W +N+ + +    ++    G       +  L + +   D+  T+ C+++N               K K  L+    F +  P   +        +  G  L ++ ++  +NP       AN      P I    D  P+      +     L++T      RK    +  F    SEG +     +DVL+    S +  D V V+  ETA     V A P    T  W   G  +      +   +   L + NVS+ D G+++   +N  A+  +     L V+  P  R++  P           TA L C A   P    +FSW  MG     E E DE  EE ED+   +             L I  V+    G Y C A N  GIS
Sbjct:  146 GEQYTVTCIAPDAKPPAEITIFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRAS----SVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVPFEPAEVTLLGSFEAV---EGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELI---VYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERG------VSRELLLTLHPSDNLATYRCDSSN---------------KAKKVLSAQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPT------ANISWSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLEPHHWRKRITCQ-AFSNVLSEGVNNF-YSLDVLFPPEFSAEQPDEVQVIEDETATLPAKVSANPDEI-TCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSN--AEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGD----TADLLCVADANPITSSMFSWKWMG-----EGERDELGEESEDDGTGV-------------LTIYEVTRDRAGVYQCTADN--GIS 803          

HSP 3 Score: 54.299 bits (129), Expect = 8.062e-6
Identity = 50/195 (25.64%), Postives = 91/195 (46.67%), Query Frame = 0
Query:  198 EGQSRRLTCISMAGNPLATLTWYR-GDEIIQGT--TQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKP-ITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSG--PFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            EG+   +TCI+    P A +T +R G+E+++    T    + + +N   +E+ + A  SD+     C A NPA+ +    L + +T+ V F P   + +  + E  KAG    + C +   NP A + W +N        GE+   S    F     ++VL ++V+  D+     C++ N+     ++   T+TV
Sbjct:  145 EGEQYTVTCIAPDAKPPAEITIFRDGEELLESESYTMSGSQDKLQN-THAEVTIRARSSDDTRRLTCRAKNPASPRA---LETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKN--------GEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTV 327          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:NPHS1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0005886 "plasma membrane" evidence=ISS] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0007254 "JNK cascade" evidence=IEA] [GO:0007519 "skeletal muscle tissue development" evidence=IEA] [GO:0007520 "myoblast fusion" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0017022 "myosin binding" evidence=IEA] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IEA] [GO:0032836 "glomerular basement membrane development" evidence=IEA] [GO:0036057 "slit diaphragm" evidence=ISS] [GO:0042995 "cell projection" evidence=IEA] [GO:0044062 "regulation of excretion" evidence=IEA] [GO:0072015 "glomerular visceral epithelial cell development" evidence=IEA] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 GO:GO:0016021 GO:GO:0005886 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0042995 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GeneTree:ENSGT00550000074545 GO:GO:0036057 TreeFam:TF327139 GO:GO:0044062 OMA:NVNEGSQ OrthoDB:EOG7RBZ7J EMBL:AAEX03001005 Ensembl:ENSCAFT00000010890 Uniprot:F1PJZ9)

HSP 1 Score: 333.954 bits (855), Expect = 2.782e-94
Identity = 261/901 (28.97%), Postives = 415/901 (46.06%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQ--GTTQDDDETETKNYAQS------ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRT-----SMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEE-SPANS-KAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPE-DKDVERTISQ-----GIILTITL--VSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKS 871
            GQ++ L+CV+ GGNP A + W +  + +  ++ T   E+  R+M   V   ED+  R  C+A N+VS       + L V F P  +TI G    +    ++  CI+ +S P  +++W +  R       +  +   GG  + SN++ L+   D    ++C A +     ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +LTW +    +      Q+    +  +  +S      EL L    SDN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+      RS PF G+     + + + +RDHG    C+A + EL E+++          +F    ++ +  P F  +    + +VE+G  L+  +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +++DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G + E ++      +  G+    L +++     AG + C  +NG+A         L+VR  P++E  +P N   AA D   +A L CRA+G P++VF+W++ G  +          ++ED +Y  ++         S L I NVS+ +DY  + C A N  G     + L   SRPD PT L V + T  SV L W PGFDGG  Q F IRY   G+  + Y+DV      +F +  L+  T+Y   + A N LGDS  T +   +   +   G+ +P  + D +    Q     G+ L   L  V GLLL+ N+  +  ++ +RR+ R+     AEG S    T S
Sbjct:  258 GQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKA---------GQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDK-EGERLESVA-TPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMS----------AFYR--LNVLYPPEFLGEQVLVVTAVEQGEALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTG-RLRIHHAKLAQAGAYQCIVDNGVAP-PARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPL----------DLEDPRY-TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITT--PGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRL-----AEGISEKTETGS 1105          

HSP 2 Score: 108.997 bits (271), Expect = 1.489e-22
Identity = 167/759 (22.00%), Postives = 291/759 (38.34%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQI-DFSYYTTGRESRNMYTFVAS------SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP----TEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRG-DEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVP--KAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAG-RYKFKVNNSEGKSRLKI--KIDVLYGATISDITDAVMVVT----GETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-----LTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGI---SRYAVYLNVTSRPDT--PTLLN 715
            GQ   + CVS    P  ++ +++  + + D S          ++T  A+      S DN +   C+  +     P+   + ++V F P    IE P         G +L   C+    NPPA +QW+ + +     +     +E      RS + +++ P D    +SC A N+       +  T+ V +PP   +I G        E ++  L+CIS +  P   L W+ G  +++       D     + + S L       DNG+   CEA + A  +E+     ++TL+V++    + ++  PE    +AG +  L C     NP  ++ W ++     E    Q   RV+ G     G+  +  L +     D+   F C+A            ++ + +      P    I      + P D +         +  ISV +NP  V   W +   E + ++     + P   S      +      D G R   + ++ E +  +    +++VLY      + + V+VVT    GE A     V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+        L V   P I       +A  D  +  +     + C     P +  +FSW R+G       EE+E + ++D +   K +          L I +      G+Y CI  N  G+   +R  V L V   P    PT LN
Sbjct:  153 GQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWG----TEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILG---SASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKET--FKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQ----------LSASTQLVVQFPPTNVTILANATALRPGDALN--------LTCISVSSNP-PVNLSW-DKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEF--LGEQVLVVTAVEQGE-ALLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETL----VRLDVHYAPTI-------RALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLG-------EEEEDQSLDDMEKISKGST-------GRLRIHHAKLAQAGAYQCIVDN--GVAPPARGLVRLVVRFAPQVEHPTPLN 847          

HSP 3 Score: 87.8113 bits (216), Expect = 4.197e-16
Identity = 103/407 (25.31%), Postives = 169/407 (41.52%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRG------DEQIDF--SYYTTGRESRNMYTFVASSED--NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV----GDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATA--QESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEEL-GESVNDGITMTVK 387
            G T  L C  H   P + V W +       D +I     Y   G  SR  +     + D  ++  Y C+  ++    E ++  ++LSV   P  + +     + V    G      C++ ++ P   + +    ++  + +  V   S+   + T +   V    +D  +++ C   +  L   +  + T++VL+PPG P I   G+D G +   GQS  L C++  GNP ATL W +  + +  T    +  E    A+S L +     D+G    CEA N  +   QE G     +TL V F P  I +          K   L C + SS P   V+ R   G       E  V  G  GG+++ + L   V   D+G+T  CEA +E    E+    +T+ VK
Sbjct:   46 GATAELQCGVHA--PGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVR-AGQSLELLCVARGGNPPATLQWLKNGQPMS-TAWGTEHAEA--VARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERG-----VTLQVTFPPSAITILGSASQ-SENKNVTLSCISKSSRP--RVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVK 438          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:NPHS1 "Nephrin" species:9606 "Homo sapiens" [GO:0005515 "protein binding" evidence=IPI] [GO:0005886 "plasma membrane" evidence=ISS;IDA;TAS] [GO:0005887 "integral component of plasma membrane" evidence=TAS] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0007254 "JNK cascade" evidence=IEA] [GO:0007519 "skeletal muscle tissue development" evidence=IEA] [GO:0007520 "myoblast fusion" evidence=IEA] [GO:0007588 "excretion" evidence=TAS] [GO:0017022 "myosin binding" evidence=IPI] [GO:0019904 "protein domain specific binding" evidence=IEA] [GO:0030507 "spectrin binding" evidence=IEA] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IEA] [GO:0032836 "glomerular basement membrane development" evidence=IEP] [GO:0036057 "slit diaphragm" evidence=ISS] [GO:0042995 "cell projection" evidence=IEA] [GO:0043234 "protein complex" evidence=IEA] [GO:0044062 "regulation of excretion" evidence=IEA] [GO:0045121 "membrane raft" evidence=IEA] [GO:0051393 "alpha-actinin binding" evidence=IEA] [GO:0070062 "extracellular vesicular exosome" evidence=IDA] [GO:0072015 "glomerular visceral epithelial cell development" evidence=IEP] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 GO:GO:0070062 GO:GO:0043234 GO:GO:0005887 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0045121 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0007588 GO:GO:0042995 Reactome:REACT_111155 Orphanet:93213 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GO:GO:0036057 TreeFam:TF327139 EMBL:AC002133 GO:GO:0044062 EMBL:AF035835 EMBL:EU642886 EMBL:AF190637 EMBL:AF126957 PIR:T37190 RefSeq:NP_004637.1 UniGene:Hs.122186 ProteinModelPortal:O60500 SMR:O60500 BioGrid:110928 IntAct:O60500 STRING:9606.ENSP00000368190 PhosphoSite:O60500 PaxDb:O60500 PRIDE:O60500 DNASU:4868 Ensembl:ENST00000353632 Ensembl:ENST00000378910 GeneID:4868 KEGG:hsa:4868 UCSC:uc002oby.3 CTD:4868 GeneCards:GC19M036316 HGNC:HGNC:7908 HPA:CAB035555 MIM:256300 MIM:602716 neXtProt:NX_O60500 Orphanet:839 Orphanet:93214 Orphanet:93216 PharmGKB:PA31709 eggNOG:NOG251936 HOGENOM:HOG000113844 HOVERGEN:HBG031752 InParanoid:O60500 OMA:NVNEGSQ OrthoDB:EOG7RBZ7J SignaLink:O60500 GeneWiki:Nephrin GenomeRNAi:4868 NextBio:18748 PRO:PR:O60500 Bgee:O60500 CleanEx:HS_NPHS1 Genevestigator:O60500 Uniprot:O60500)

HSP 1 Score: 318.931 bits (816), Expect = 2.628e-89
Identity = 238/837 (28.43%), Postives = 381/837 (45.52%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYT--TGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGT--TQDDDETETKNYAQS------ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRT-----SMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSE--NVAVETLSYLEGMVKPEDK 813
            GQ++ L CV+ GGNPLA + W +  + +  ++ T  T   +R++       ED+  +  C+A N+VS       I L V F P  + I G         ++  C++ +S P  +++W +  R          +   GG  + SN++ L    D    ++C A +     ET  ++  ++V YP  K  I G   G  +  G   RL C+++ GNP  +L WY+    +  +   Q+       +  +S      EL L    SDN   + C+A         G LS++  L+V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E       R  PF G+     + + V +RDHG    C A + EL E+V+          SF    ++ + +P F  +    + +VE+G  L+  +SV ANP    + W            L     PR  I S    +L N+T A   D G Y+    NSEG +  ++++DV Y  TI  + D   V  G +    C VDA PI     +W R+G D E ++      +  G     L +++     AG + C  +NG+A     +   L+VR  P++E     +K  AA D   +A L CRA+G P++VF+W++ G  +          +++D +Y  ++         S L I NVS+ +DY  + C A N  G  +  + L   SRPD P+ L V + T +SV L W PGFDGG  Q F IRY   G+  + YVDV      +F +  L+  T+Y   + A N LGDS    +   + + T    +   +PED+
Sbjct:  258 GQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKA---------GQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDK-EGERLEGVAA-PPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVS----------SFYR--LNVLYRPEFLGEQVLVVTAVEQGEALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSSGALHLWNVTRA---DDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGEDEEDQSLDDMEKISRGPTG-RLRIHHAKLAQAGAYQCIVDNGVAP-PARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPL----------DLQDPRY-TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQ 1048          

HSP 2 Score: 108.612 bits (270), Expect = 2.074e-22
Identity = 160/707 (22.63%), Postives = 289/707 (40.88%), Query Frame = 0
Query:   53 NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV-----GDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATA--QESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-------VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGAT----------ISDITDAVMVVTGETAHFECV-VDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGEFL-CTANNGIADMKDTKSTF--LLVRQRPR-IEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP-----DTPTLLNV 716
            ++  Y C+  ++ +  E ++  ++LS+   P K+ +  P    +     G     +C++ ++ P   +  ++  ++  + +  V   S++  +   +   V    +D  +++ C A +  L   +  + T++VL+PPG P I   G+D G +   GQS  L C++  GNPLATL W +  + +      +    T+  A+S L +T    D+G    CEA N  +A  QE G     ITL V F P  I +       +  K   L C + SS P   V+ R   G       E  V  G  GG+++ + L       D+G+T  CEA +E   +            ++F   LI  +  P   +     I    EG  L       +V +++  NP + +  W   Y +   + T+    +P+     +L ++ ++    +         S+ +++   K   L  +T          ++ + +A  +  G+  +  CV V + P     +SW + G  +EG     R +   G+ +    +  VS  D G+ + C A++  A++++T S+F  L V  RP  + E      A         ++  A   P+  F+W+  G  +S                        R+     LH+ NV+  D G Y    QN EG +   + L+V   P       PT +NV
Sbjct:  105 DDAEYECQVGRSEMGPELVSPRVILSI-LVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVR-AGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTE---HTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHG-----ITLQVTFPPSAIIILGSASQTE-NKNVTLSCVSKSSRP--RVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTK------------ETFKKSLILNVKYPAQKL----WIEGPPEGQKLRAGTRVRLVCLAIGGNP-EPSLMW---YKD---SRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPV--NLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHS--AELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPE-AFNWTFRGYRLSP-----------------AGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNV 753          

HSP 3 Score: 106.686 bits (265), Expect = 7.178e-22
Identity = 160/729 (21.95%), Postives = 279/729 (38.27%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEV-VWYRGDEQIDFSYYTTGRESRNMYTFVAS------SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP----TEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRG-DEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPK--AGKKAYLFCETGSSNPTANVIWRRNDGLSIEN---QGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAG-RYKFKVNNSEGKSRLK--IKIDVLYGATISDITDAVMVVT----GETAHFECVVDAKPITADTISWSRVGFDME---GRTSMQNGTNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-----LTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQN 691
            GQ   + CVS    P  ++ +   G    D S        + ++T  A+      S DN +   C+A +     P+ A   ++V F P    IE P         G +L   C+    NP A +QW+ + +     +     +E      RS + + + P D    +SC A N+    T     T+ V +PP    I G    T   E ++  L+C+S +  P   L W+ G  +++       D     + + S L   A   DNG+   CEA + A  +E+     ++ L+V++    + ++  PE  K  AG +  L C     NP  +++W ++     E+   Q   RV  G     G+  +  L +     D+   F C+A   +L  S    +                   P  ++       ++  G  L +  +SV +NP  V   W +   E +  +       P   S      + +    D G R   + +++E +  +    +++VLY      + + V+VVT    GE A     V A P   +  +W+  G+ +    G       + ++HL  +NV++ D G  +  C  + G A+           R R  +  +P   +A  D  +  V     + C     P +  +F+W R+G        EDE ++  D+  +I      R      L I +      G+Y CI  N
Sbjct:  153 GQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWG----TEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQT---ENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKET--FKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAG--QLSASTQLAVQF-----------------PPTNVTILANASALRPGDALNLTCVSVSSNP-PVNLSW-DKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFYRLNVLYRPEF--LGEQVLVVTAVEQGE-ALLPVSVSANP-APEAFNWTFRGYRLSPAGGPRHRILSSGALHL--WNVTRADDGLYQLHCQNSEGTAE----------ARLRLDVHYAP-TIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLG--------EDEEDQSLDDMEKISRGPTGR------LRIHHAKLAQAGAYQCIVDN 820          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:NPHS1 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0005886 "plasma membrane" evidence=ISS] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0007254 "JNK cascade" evidence=IEA] [GO:0007519 "skeletal muscle tissue development" evidence=IEA] [GO:0007520 "myoblast fusion" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0017022 "myosin binding" evidence=IEA] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IEA] [GO:0032836 "glomerular basement membrane development" evidence=IEA] [GO:0036057 "slit diaphragm" evidence=ISS] [GO:0042995 "cell projection" evidence=IEA] [GO:0044062 "regulation of excretion" evidence=IEA] [GO:0072015 "glomerular visceral epithelial cell development" evidence=IEA] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 GO:GO:0016021 GO:GO:0005886 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0042995 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GeneTree:ENSGT00550000074545 GO:GO:0036057 TreeFam:TF327139 GO:GO:0044062 CTD:4868 OMA:NVNEGSQ OrthoDB:EOG7RBZ7J EMBL:DAAA02046952 RefSeq:NP_001179441.1 UniGene:Bt.86142 PRIDE:F1MPI1 Ensembl:ENSBTAT00000010289 GeneID:519706 KEGG:bta:519706 NextBio:20872931 Uniprot:F1MPI1)

HSP 1 Score: 318.161 bits (814), Expect = 4.522e-89
Identity = 234/811 (28.85%), Postives = 371/811 (45.75%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGT--TQDDDETETKNYAQS------ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRT-----SMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDS 789
            GQ++ L+C + GGNPLA + W +  + +  ++ T   ++  R++   +   ED+  R  C+A N+VS       + L V F P  +TI G    +    ++  CIT +S P  +++W +  R  Q +     +   GG  + SN++ L    D    ++C A +     ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +LTWY+    +      Q+    +  +  +S      EL L    SDN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+    + R  PF G+     + + + +RDHG    C A + EL E+V+    + V            +  P F  +    + +VE+G  L+  +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +++DV Y  TI  + D   V  G++    C VDA PI  +  +W R+G + E +       M  G+    L +++     AG + C  +NG+A         L+VR  P +E     +K  AA D   +A L CRA+G P++VF+W++ G  +          +++D +Y  ++         S L I NVS+ +DY  + CIA N  G     + L   SRPD P+ L   + T  SV L W PGFDGG  Q F IRY    +  + Y+DV      +F +  L+  T+Y   + A N LGDS
Sbjct:  258 GQSLELLCTARGGNPLATLQWLKNGQPVSTAWGTEHAQAVARSLLVMIVRPEDHGARLSCEAYNSVSSGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCITKSSRPRVLLRWWLGWRQLQPSEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGNRVRLVCLAIGGNPDPSLTWYKDSRTVTEPRPPQEPRRVQLGSVEKSGSIFSRELVLITGPSDNQAKFTCKA---------GQLSASTQLVVQFPPTNVTILANASTLRPGDALNLTCVSVSSNPPVNLSWDK-EGERLEDVAA-QPRRAPFKGSAAARSVLLRLSSRDHGHRVTCSAHSTELRETVSAFYRLNV------------LYPPEFLGEQVLVVTAVEQGEALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGLYQLHCQNSEGTAEALVRLDVQYAPTIRALQDPTEVNVGDSVDIVCTVDANPILPEMFNWERLGEEGEDQNLDDMEKMSKGSTG-RLRIHHAKLTQAGAYQCIVDNGVAP-PARGLVRLVVRFAPHVEHPAPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPL----------DLQDPRY-TEHTYHQGGVHSSLLTIANVSAAQDYALFTCIATNPLGSDHTNIQLVSISRPDPPSGLKAVSMTPYSVGLEWKPGFDGGLPQRFQIRYEALETPGFLYMDVLPPQATTFTLTGLQPSTRYRVWLLASNALGDS 1022          

HSP 2 Score: 102.449 bits (254), Expect = 1.545e-20
Identity = 157/730 (21.51%), Postives = 279/730 (38.22%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID-FSYYTTGRESRNMYTFVAS------SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP----TEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVP--KAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAG-RYKFKVNNSEGKSRLKI--KIDVLYGATISDITDAVMVVT----GETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-----LTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQN 691
            GQ  ++ CVS    P  ++ +    + I   S        + ++T  A+      S DN +   C+  +     P+ A   ++V F P    IE P     +   G +L   C     NP A +QW+ + +     +     +E      RS + +++ P D    +SC A N+       +  T+ V +PP   +I G        E ++  L+CI+ +  P   L W+ G   +Q + +   D     + + S L   A   DNG+   CEA + A  +E+     ++TL+V++    + ++  PE    +AG +  L C     NP  ++ W ++     E    Q   RV+ G     G++ +  L +     D+   F C+A            ++ + +      P    I      + P D +         +  +SV +NP  V   W +   E + ++       P   S      +      D G R     +++E +  +    +++VLY      + + V+VVT    GE A     V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+        L V+  P I       +A  D  +  V     + C     P +  +F+W R+G       EE E + ++D +   K +          L I +      G+Y CI  N
Sbjct:  153 GQEYTVSCVSGDAKPAPDITFLLSGQTISGISANVNEGSQQKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIKASFTMNVLFPPGPPVIEWPGLDEGQVRAGQSLELLCTARGGNPLATLQWLKNGQPVSTAWG----TEHAQAVARSLLVMIVRPEDHGARLSCEAYNSVSSGIQERGVTLQVTFPPSAITILG---SASQSENKNVTLSCITKSSRPRVLLRWWLGWRQLQPSEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKET--FKKSLTLNVKYPAQKLWIEGPPEGQRLRAGNRVRLVCLAIGGNPDPSLTWYKDSRTVTEPRPPQEPRRVQLGSVEKSGSIFSRELVLITGPSDNQAKFTCKAGQ----------LSASTQLVVQFPPTNVTILANASTLRPGDALN--------LTCVSVSSNP-PVNLSW-DKEGERLEDVAAQPRRAPFKGSAAARSVLLRLSSRDHGHRVTCSAHSTELRETVSAFYRLNVLYPPEF--LGEQVLVVTAVEQGE-ALLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAEAL----VRLDVQYAPTI-------RALQDPTEVNVGDSVDIVCTVDANPILPEMFNWERLG-------EEGEDQNLDDMEKMSKGST-------GRLRIHHAKLTQAGAYQCIVDN 820          

HSP 3 Score: 94.7449 bits (234), Expect = 3.075e-18
Identity = 153/699 (21.89%), Postives = 277/699 (39.63%), Query Frame = 0
Query:   53 NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV----GDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATA--QESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEEL-GESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRW-------ANPYNENIP-NITLDS-DNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECV-VDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTF--LLVRQRPR-IEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP-----DTPTLLNV 716
            ++  Y C+  ++    E ++  ++LS+   P  + +     + V    G   +  C++ ++ P   + +++  ++    +  V   S++  + T +   V    +D  +++ C   +  L   +  + T++VL+PPG P I   G+D G +   GQS  L C +  GNPLATL W +  + +      +     +  A+S L +     D+G    CEA N  ++  QE G     +TL V F P  I +          K   L C T SS P   V+ R   G       E  V  G  GG+++ + L       D+G+T  CEA +E    E+    +T+ VK          +  + ++   P +           +V +++  NP D +  W         P     P  + L S +    I+S + L+ IT    ++  ++  K       ++L ++        ++ + +A  +  G+  +  CV V + P     +SW + G  +E      R +   G+ +    +  +S  D G    C+A++   ++++T S F  L V   P  + E      A         ++  A   P+  F+W+  G  +S                        R+     L + NV+  D G Y    QN EG +   V L+V   P       PT +NV
Sbjct:  105 DDAEYECQVGRSETGPELVSPRVILSILVPPKVLQLTPEAGSTVTWVAGQEYTVSCVSGDAKPAPDITFLLSGQTISGISANVNEGSQQKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIKASFTMNVLFPPGPPVIEWPGLDEGQVR-AGQSLELLCTARGGNPLATLQWLKNGQPVSTAWGTE---HAQAVARSLLVMIVRPEDHGARLSCEAYNSVSSGIQERG-----VTLQVTFPPSAITILGSASQ-SENKNVTLSCITKSSRP--RVLLRWWLGWRQLQPSEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLTLNVK----------YPAQKLWIEGPPEGQRLRAGNRVRLVCLAIGGNP-DPSLTWYKDSRTVTEPRPPQEPRRVQLGSVEKSGSIFS-RELVLIT-GPSDNQAKFTCKAGQLSASTQLVVQFP---PTNVTILANASTLRPGDALNLTCVSVSSNPPV--NLSWDKEGERLEDVAAQPRRAPFKGSAAARSVLLRLSSRDHGHRVTCSAHS--TELRETVSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPE-AFNWTFRGYRLSP-----------------AGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAEALVRLDVQYAPTIRALQDPTEVNV 753          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:Nphs1 "nephrosis 1, nephrin" species:10090 "Mus musculus" [GO:0000165 "MAPK cascade" evidence=IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=IDA] [GO:0005886 "plasma membrane" evidence=ISO;IDA] [GO:0005887 "integral component of plasma membrane" evidence=TAS] [GO:0007155 "cell adhesion" evidence=IGI] [GO:0007254 "JNK cascade" evidence=IDA] [GO:0007275 "multicellular organismal development" evidence=IEA] [GO:0007517 "muscle organ development" evidence=IEA] [GO:0007519 "skeletal muscle tissue development" evidence=IMP] [GO:0007520 "myoblast fusion" evidence=IMP] [GO:0016020 "membrane" evidence=ISO] [GO:0016021 "integral component of membrane" evidence=TAS] [GO:0017022 "myosin binding" evidence=ISO] [GO:0019904 "protein domain specific binding" evidence=ISO] [GO:0030507 "spectrin binding" evidence=ISO] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IGI] [GO:0036057 "slit diaphragm" evidence=ISO] [GO:0042995 "cell projection" evidence=IDA] [GO:0043234 "protein complex" evidence=ISO] [GO:0044062 "regulation of excretion" evidence=IMP] [GO:0045121 "membrane raft" evidence=ISO] [GO:0051393 "alpha-actinin binding" evidence=ISO] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 MGI:MGI:1859637 GO:GO:0043234 GO:GO:0005887 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0045121 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0042995 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GeneTree:ENSGT00550000074545 GO:GO:0036057 TreeFam:TF327139 EMBL:AC167970 GO:GO:0044062 CTD:4868 eggNOG:NOG251936 HOVERGEN:HBG031752 OMA:NVNEGSQ OrthoDB:EOG7RBZ7J EMBL:AF168466 EMBL:AF172254 EMBL:AF172247 EMBL:AF172248 EMBL:AF172249 EMBL:AF172250 EMBL:AF172251 EMBL:AF172252 EMBL:AF172253 EMBL:AF172256 EMBL:AF190638 EMBL:AF191090 EMBL:AB513652 EMBL:AY183460 RefSeq:NP_062332.2 RefSeq:XP_006540278.1 UniGene:Mm.437830 ProteinModelPortal:Q9QZS7 SMR:Q9QZS7 BioGrid:207694 STRING:10090.ENSMUSP00000006825 PhosphoSite:Q9QZS7 PaxDb:Q9QZS7 PRIDE:Q9QZS7 DNASU:54631 Ensembl:ENSMUST00000006825 GeneID:54631 KEGG:mmu:54631 UCSC:uc009gem.1 UCSC:uc009gen.1 InParanoid:Q9ET59 NextBio:311448 PRO:PR:Q9QZS7 ArrayExpress:Q9QZS7 Bgee:Q9QZS7 CleanEx:MM_NPHS1 Genevestigator:Q9QZS7 Uniprot:Q9QZS7)

HSP 1 Score: 313.153 bits (801), Expect = 2.343e-87
Identity = 244/845 (28.88%), Postives = 383/845 (45.33%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDD---------ETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE------IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFD-----MEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK-PEDKD 814
            G+ + L C++ GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS E     I L V F P  VTI G T  +    ++  C+T +S P  +++W +  R          +   GG  + SN+++L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +LTW +    +    Q  +         E     +++ EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N        LS++ +GE       + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF    ++ +  P F  +    +  VE+G  L+  +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G D     ++    M  G+    L +       AG + C  +NG+A         L+VR  P+++     +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E KY+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    S  + Y+DV  +   +F +  LK  T+Y   + A N LGDS  T + + V   +   G+ + PED D
Sbjct:  272 GENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSR-ELVLIIGPPDNLAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVN--------LSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVS----------SFYR--LNVLYPPEFLGEQVRAVTVVEQGQALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEDEEELNLDDMEKMSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHKYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITT--PGLDQAPEDTD 1060          

HSP 2 Score: 109.768 bits (273), Expect = 8.056e-23
Identity = 90/387 (23.26%), Postives = 165/387 (42.64%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID--FSYYTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVP--KAGKKAYLFCETGSSNPTANVIWRR-----NDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEA 369
            GQ   + CVS    P  ++++ +G   ++   S    G E +  +T         S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   CI    NPPA +QW+ + +      ++   +E       S + + + P D    +SC + N+   ET  ++ T+ V +PP   +I G    T   E ++  L C++ +  P   L W+ G   +  T +   D     + + S L L     DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++ W +     ND    +    +++ S    G+  +  L + +   D+   F C+A
Sbjct:  167 GQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCIARGGNPPATLQWLKNGKPV----SIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILG---STSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKA 544          

HSP 3 Score: 63.5438 bits (153), Expect = 1.059e-8
Identity = 60/236 (25.42%), Postives = 101/236 (42.80%), Query Frame = 0
Query:  159 CYAINNDLGETVVQTHTI-SVLYPPGKPSITGIDPGTI-MDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDE--TETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKP----ITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            C    ++LG  +V    I S+L  P    +T     T+    GQ   +TC+S    P   + + +G   ++  +   +E   E   + ++E  +T   SDNG +  CE SNPA A    P+ ++ T+++ F P    I     ++  V +AG+   L C     NP A + W +N        G    ++      +  +VL + V   DHG    C++ N    E+    IT+ V
Sbjct:  125 CQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILFPPGPPVIDWPGLNEGHV-RAGENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQA------VAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQV 350          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:Nphs1 "Nephrin" species:10116 "Rattus norvegicus" [GO:0016021 "integral component of membrane" evidence=IEA] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 RGD:620460 GO:GO:0016021 Gene3D:2.60.40.10 InterPro:IPR013783 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 InterPro:IPR013162 Pfam:PF08205 GeneTree:ENSGT00550000074545 EMBL:AABR06004284 EMBL:AABR06004285 EMBL:AABR06004286 ProteinModelPortal:D4A8H0 Ensembl:ENSRNOT00000028358 NextBio:35573359 ArrayExpress:D4A8H0 Uniprot:D4A8H0)

HSP 1 Score: 302.753 bits (774), Expect = 3.763e-84
Identity = 228/821 (27.77%), Postives = 371/821 (45.19%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETK--------NYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAV 798
            G+ + L C + GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS       I L V F P  +TI G    +    ++  C+T +S P  +++W +  R          +   GG  + SN++ L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +L W++    +    Q  +    +        +    EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+    + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF     + +  P F  +    +  VE+G  +++ +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G      +++    +  G+    L +       AG + C  +NG+A         L+VR  P++++    +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E +Y+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    +  + +VDV  +   +F +  LK  T+Y   + A N LGDS  T + + V
Sbjct:  272 GENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDK-EGERLEDVAA-KPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVS----------SFYR--FNVLYPPEFLGEQVRAVTVVEQG-QVLLPVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHRYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV 1045          

HSP 2 Score: 97.8265 bits (242), Expect = 4.007e-19
Identity = 164/754 (21.75%), Postives = 283/754 (37.53%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYR-GDEQIDFSY-YTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD-TIF----SVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK---IKIDVLYGAT-ISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRP--DTPTLL 714
            GQ   + CVS    P  ++ + + G   +D S     G E +   T      +  S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   C     NPPA +QW+ + +     +     +E       S + + + P D    +SC + N+    T  ++ T+ V +PP   +I G        E ++  L C++ +  P   L W+ G   +  T +   D     + + S L       DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++IW ++     E    Q   RV+ G     G+  +  L + +   D+   F C+A   +L  S                       + +    PT+ TI     ++  G  L +  +S+ +NP  V   W +   E + ++     + P   S  +          D G+      +SE          + +VLY    + +   AV VV          V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+             R   + +  N   + D      + C     P +  +FSW R+G       EE+E   ++D +   K +          L I+       G+Y CI  N     +R  V L V   P  D PT L
Sbjct:  167 GQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWG----TEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILG---SVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAG--QLSAST----------------------QLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNP-PVNLSW-DKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVD------IVCTVDANPILPEMFSWERLG-------EEEEDLNLDDMEKVSKGST-------GRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPL 860          

HSP 3 Score: 85.8853 bits (211), Expect = 1.548e-15
Identity = 86/367 (23.43%), Postives = 158/367 (43.05%), Query Frame = 0
Query:   33 YYTTGRESRNMYTFVASSED--NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN-----VGDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELG-ESVNDGITMTVK 387
            Y   G  ++  +  +  + D  ++  Y C+  ++ +  E ++  ++LS+  +P KV +  P   +      G      C++ ++ P   + ++   R+  + +  V   SE     T +   V+   +D  +++ C   N  L   +  + T+++L+PPG P I   G++ G +   G++  L C +  GNP ATL W +  + +      +     +  A S L +T    D+G    C++ N  +A   G    +ITL V F P  I +          K   L C T SS P   + W    G  +    E  V  G  GG+++ + L   V   D+G+   CEA ++    E+    +T+ VK
Sbjct:   97 YSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSP-KVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVR-AGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTE---HAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSA---GTQERSITLQVTFPPSAITILGSVSQ-SENKNVTLCCLTKSSRPRVLLRWWLG-GRQLLPTDET-VMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVK 452          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:Nphs1 "Nephrin" species:10116 "Rattus norvegicus" [GO:0003094 "glomerular filtration" evidence=IC] [GO:0005515 "protein binding" evidence=IPI] [GO:0005886 "plasma membrane" evidence=IDA;TAS] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0007254 "JNK cascade" evidence=IEA] [GO:0007519 "skeletal muscle tissue development" evidence=IEA] [GO:0007520 "myoblast fusion" evidence=IEA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0017022 "myosin binding" evidence=IEA] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IEA] [GO:0032836 "glomerular basement membrane development" evidence=IEA] [GO:0036057 "slit diaphragm" evidence=IDA] [GO:0042995 "cell projection" evidence=IEA] [GO:0044062 "regulation of excretion" evidence=IEA] [GO:0072015 "glomerular visceral epithelial cell development" evidence=IEA] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 RGD:620460 GO:GO:0016021 GO:GO:0005886 GO:GO:0043234 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0045121 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0042995 Reactome:REACT_197471 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 GO:GO:0003094 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GeneTree:ENSGT00550000074545 GO:GO:0036057 GO:GO:0044062 CTD:4868 eggNOG:NOG251936 HOGENOM:HOG000113844 HOVERGEN:HBG031752 OrthoDB:EOG7RBZ7J EMBL:AF172255 EMBL:AF161715 EMBL:AF125521 RefSeq:NP_072150.1 UniGene:Rn.48745 ProteinModelPortal:Q9R044 BioGrid:249144 IntAct:Q9R044 MINT:MINT-8384233 PhosphoSite:Q9R044 PaxDb:Q9R044 PRIDE:Q9R044 Ensembl:ENSRNOT00000045212 GeneID:64563 KEGG:rno:64563 UCSC:RGD:620460 InParanoid:Q9JIX2 NextBio:613494 PRO:PR:Q9R044 Genevestigator:Q9R044 Uniprot:Q9R044)

HSP 1 Score: 302.368 bits (773), Expect = 7.368e-84
Identity = 228/821 (27.77%), Postives = 371/821 (45.19%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETK--------NYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAV 798
            G+ + L C + GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS       I L V F P  +TI G    +    ++  C+T +S P  +++W +  R          +   GG  + SN++ L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +L W++    +    Q  +    +        +    EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+    + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF     + +  P F  +    +  VE+G  +++ +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G      +++    +  G+    L +       AG + C  +NG+A         L+VR  P++++    +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E +Y+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    +  + +VDV  +   +F +  LK  T+Y   + A N LGDS  T + + V
Sbjct:  272 GENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDK-EGERLEDVAA-KPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVS----------SFYR--FNVLYPPEFLGEQVRAVTVVEQG-QVLLPVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHRYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV 1045          

HSP 2 Score: 97.4413 bits (241), Expect = 4.848e-19
Identity = 164/754 (21.75%), Postives = 283/754 (37.53%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYR-GDEQIDFSY-YTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD-TIF----SVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK---IKIDVLYGAT-ISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRP--DTPTLL 714
            GQ   + CVS    P  ++ + + G   +D S     G E +   T      +  S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   C     NPPA +QW+ + +     +     +E       S + + + P D    +SC + N+    T  ++ T+ V +PP   +I G        E ++  L C++ +  P   L W+ G   +  T +   D     + + S L       DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++IW ++     E    Q   RV+ G     G+  +  L + +   D+   F C+A   +L  S                       + +    PT+ TI     ++  G  L +  +S+ +NP  V   W +   E + ++     + P   S  +          D G+      +SE          + +VLY    + +   AV VV          V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+             R   + +  N   + D      + C     P +  +FSW R+G       EE+E   ++D +   K +          L I+       G+Y CI  N     +R  V L V   P  D PT L
Sbjct:  167 GQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWG----TEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILG---SVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAG--QLSAST----------------------QLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNP-PVNLSW-DKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVD------IVCTVDANPILPEMFSWERLG-------EEEEDLNLDDMEKVSKGST-------GRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPL 860          

HSP 3 Score: 86.2705 bits (212), Expect = 1.466e-15
Identity = 86/367 (23.43%), Postives = 159/367 (43.32%), Query Frame = 0
Query:   33 YYTTGRESRNMYTFVASSED--NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN-----VGDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELG-ESVNDGITMTVK 387
            Y   G  ++  +  +  + D  ++  Y C+  ++ +  E ++  ++LS+  +P KV +  P   +      G      C++ ++ P   + ++   R+  + +  V   SE     T +   V+   +D  +++ C   N  L   +  + T+++L+PPG P I   G++ G +   G++  L C +  GNP ATL W +  + +      +     +  A S L +T    D+G    C++ N  +A   G    +ITL V F P  I +       +  K   L C T SS P   + W    G  +    E  V  G  GG+++ + L   V   D+G+   CEA ++    E+    +T+ VK
Sbjct:   97 YSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSP-KVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVR-AGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTE---HAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSA---GTQERSITLQVTFPPSAITILGSVSQSE-NKNVTLCCLTKSSRPRVLLRWWLG-GRQLLPTDET-VMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVK 452          
BLAST of EMLSAG00000004534 vs. GO
Match: - (symbol:Nphs1 "nephrosis 1, congenital, Finnish type (nephrin)" species:10116 "Rattus norvegicus" [GO:0000165 "MAPK cascade" evidence=ISO] [GO:0003094 "glomerular filtration" evidence=IC] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=ISO] [GO:0005886 "plasma membrane" evidence=ISO;IDA;TAS] [GO:0007155 "cell adhesion" evidence=IEA;ISO] [GO:0007254 "JNK cascade" evidence=IEA;ISO] [GO:0007519 "skeletal muscle tissue development" evidence=IEA;ISO] [GO:0007520 "myoblast fusion" evidence=IEA;ISO] [GO:0016020 "membrane" evidence=IDA] [GO:0016021 "integral component of membrane" evidence=IEA] [GO:0017022 "myosin binding" evidence=IEA;ISO] [GO:0019904 "protein domain specific binding" evidence=IPI] [GO:0030507 "spectrin binding" evidence=IPI] [GO:0030838 "positive regulation of actin filament polymerization" evidence=IEA;ISO] [GO:0032836 "glomerular basement membrane development" evidence=IEA;ISO] [GO:0036057 "slit diaphragm" evidence=IDA] [GO:0042995 "cell projection" evidence=IEA;ISO] [GO:0043234 "protein complex" evidence=IDA] [GO:0044062 "regulation of excretion" evidence=IEA;ISO] [GO:0045121 "membrane raft" evidence=IDA] [GO:0051393 "alpha-actinin binding" evidence=IPI] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] [GO:0072015 "glomerular visceral epithelial cell development" evidence=IEA;ISO] InterPro:IPR003961 InterPro:IPR007110 Pfam:PF00041 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 RGD:620460 GO:GO:0016021 GO:GO:0005886 GO:GO:0043234 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0045121 GO:GO:0007254 SUPFAM:SSF49265 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0007155 GO:GO:0042995 Reactome:REACT_197471 GO:GO:0007520 GO:GO:0030838 InterPro:IPR013098 Pfam:PF07679 InterPro:IPR013106 Pfam:PF07686 GO:GO:0003094 InterPro:IPR013162 Pfam:PF08205 GO:GO:0072015 GO:GO:0007519 GO:GO:0032836 GeneTree:ENSGT00550000074545 GO:GO:0036057 GO:GO:0044062 CTD:4868 eggNOG:NOG251936 HOGENOM:HOG000113844 HOVERGEN:HBG031752 OrthoDB:EOG7RBZ7J EMBL:AF172255 EMBL:AF161715 EMBL:AF125521 RefSeq:NP_072150.1 UniGene:Rn.48745 ProteinModelPortal:Q9R044 BioGrid:249144 IntAct:Q9R044 MINT:MINT-8384233 PhosphoSite:Q9R044 PaxDb:Q9R044 PRIDE:Q9R044 Ensembl:ENSRNOT00000045212 GeneID:64563 KEGG:rno:64563 UCSC:RGD:620460 InParanoid:Q9JIX2 NextBio:613494 PRO:PR:Q9R044 Genevestigator:Q9R044 Uniprot:Q9R044)

HSP 1 Score: 302.368 bits (773), Expect = 7.368e-84
Identity = 228/821 (27.77%), Postives = 371/821 (45.19%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETK--------NYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAV 798
            G+ + L C + GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS       I L V F P  +TI G    +    ++  C+T +S P  +++W +  R          +   GG  + SN++ L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +L W++    +    Q  +    +        +    EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+    + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF     + +  P F  +    +  VE+G  +++ +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G      +++    +  G+    L +       AG + C  +NG+A         L+VR  P++++    +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E +Y+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    +  + +VDV  +   +F +  LK  T+Y   + A N LGDS  T + + V
Sbjct:  272 GENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDK-EGERLEDVAA-KPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVS----------SFYR--FNVLYPPEFLGEQVRAVTVVEQG-QVLLPVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHRYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV 1045          

HSP 2 Score: 97.4413 bits (241), Expect = 4.848e-19
Identity = 164/754 (21.75%), Postives = 283/754 (37.53%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYR-GDEQIDFSY-YTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD-TIF----SVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK---IKIDVLYGAT-ISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRP--DTPTLL 714
            GQ   + CVS    P  ++ + + G   +D S     G E +   T      +  S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   C     NPPA +QW+ + +     +     +E       S + + + P D    +SC + N+    T  ++ T+ V +PP   +I G        E ++  L C++ +  P   L W+ G   +  T +   D     + + S L       DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++IW ++     E    Q   RV+ G     G+  +  L + +   D+   F C+A   +L  S                       + +    PT+ TI     ++  G  L +  +S+ +NP  V   W +   E + ++     + P   S  +          D G+      +SE          + +VLY    + +   AV VV          V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+             R   + +  N   + D      + C     P +  +FSW R+G       EE+E   ++D +   K +          L I+       G+Y CI  N     +R  V L V   P  D PT L
Sbjct:  167 GQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWG----TEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILG---SVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAG--QLSAST----------------------QLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNP-PVNLSW-DKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVD------IVCTVDANPILPEMFSWERLG-------EEEEDLNLDDMEKVSKGST-------GRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPL 860          

HSP 3 Score: 86.2705 bits (212), Expect = 1.466e-15
Identity = 86/367 (23.43%), Postives = 159/367 (43.32%), Query Frame = 0
Query:   33 YYTTGRESRNMYTFVASSED--NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN-----VGDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELG-ESVNDGITMTVK 387
            Y   G  ++  +  +  + D  ++  Y C+  ++ +  E ++  ++LS+  +P KV +  P   +      G      C++ ++ P   + ++   R+  + +  V   SE     T +   V+   +D  +++ C   N  L   +  + T+++L+PPG P I   G++ G +   G++  L C +  GNP ATL W +  + +      +     +  A S L +T    D+G    C++ N  +A   G    +ITL V F P  I +       +  K   L C T SS P   + W    G  +    E  V  G  GG+++ + L   V   D+G+   CEA ++    E+    +T+ VK
Sbjct:   97 YSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSP-KVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVR-AGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTE---HAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSA---GTQERSITLQVTFPPSAITILGSVSQSE-NKNVTLCCLTKSSRPRVLLRWWLG-GRQLLPTDET-VMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVK 452          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847736|gb|GAXK01109808.1| (TSA: Calanus finmarchicus comp225879_c0_seq8 transcribed RNA sequence)

HSP 1 Score: 654.825 bits (1688), Expect = 0.000e+0
Identity = 376/918 (40.96%), Postives = 539/918 (58.71%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLS--YLEGMVKPEDKDVERTISQGIILT-ITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+    +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T      E +          + S G +LT I  + G+L I N+ L+ CYVKRR    + G S         S+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESG--TFVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKGDKSEPIKPTPTPPKSNSGSGGNVLTIIATIGGILFICNLCLLYCYVKRRAGKHLFGGSSRG------SSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3725          

HSP 2 Score: 111.309 bits (277), Expect = 1.149e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847737|gb|GAXK01109807.1| (TSA: Calanus finmarchicus comp225879_c0_seq7 transcribed RNA sequence)

HSP 1 Score: 654.825 bits (1688), Expect = 0.000e+0
Identity = 376/918 (40.96%), Postives = 539/918 (58.71%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLS--YLEGMVKPEDKDVERTISQGIILT-ITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+    +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T      E +          + S G +LT I  + G+L I N+ L+ CYVKRR    + G S         S+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESG--TFVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKGDKSEPIKPTPTPPKSNSGSGGNVLTIIATIGGILFICNLCLLYCYVKRRAGKHLFGGSSRG------SSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3725          

HSP 2 Score: 111.309 bits (277), Expect = 1.150e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847738|gb|GAXK01109806.1| (TSA: Calanus finmarchicus comp225879_c0_seq6 transcribed RNA sequence)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 378/919 (41.13%), Postives = 546/919 (59.41%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETL-SYLEGMVKPEDKDV--ERTISQGIILTITLVSGL-LLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+    +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T  S   G  +     V  ER     II+    V+G+ LLILNV+L+ C+++ R+   +  +     GS+ GS+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESG--TFVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKSPPPGARQQMINSVLEERAEIPFIIIASVSVAGIFLLILNVVLLYCFIQNRKTENIKDNLSLG-GSSRGSSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3743          

HSP 2 Score: 111.309 bits (277), Expect = 1.252e-23
Identity = 159/749 (21.23%), Postives = 293/749 (39.12%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I  +            +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSK-----SKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847739|gb|GAXK01109805.1| (TSA: Calanus finmarchicus comp225879_c0_seq5 transcribed RNA sequence)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 378/919 (41.13%), Postives = 546/919 (59.41%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETL-SYLEGMVKPEDKDV--ERTISQGIILTITLVSGL-LLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+    +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T  S   G  +     V  ER     II+    V+G+ LLILNV+L+ C+++ R+   +  +     GS+ GS+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESG--TFVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKSPPPGARQQMINSVLEERAEIPFIIIASVSVAGIFLLILNVVLLYCFIQNRKTENIKDNLSLG-GSSRGSSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3743          

HSP 2 Score: 111.309 bits (277), Expect = 1.253e-23
Identity = 159/749 (21.23%), Postives = 293/749 (39.12%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I  +            +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSK-----SKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847740|gb|GAXK01109804.1| (TSA: Calanus finmarchicus comp225879_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 377/918 (41.07%), Postives = 540/918 (58.82%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLS--YLEGMVKPEDKDVERTISQGIILT-ITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+ T  +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T      E +          + S G +LT I  + G+L I N+ L+ CYVKRR    + G S         S+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGT--FVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKGDKSEPIKPTPTPPKSNSGSGGNVLTIIATIGGILFICNLCLLYCYVKRRAGKHLFGGSSRG------SSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3725          

HSP 2 Score: 110.923 bits (276), Expect = 1.513e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847741|gb|GAXK01109803.1| (TSA: Calanus finmarchicus comp225879_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 377/918 (41.07%), Postives = 540/918 (58.82%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLS--YLEGMVKPEDKDVERTISQGIILT-ITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+ T  +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T      E +          + S G +LT I  + G+L I N+ L+ CYVKRR    + G S         S+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGT--FVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKGDKSEPIKPTPTPPKSNSGSGGNVLTIIATIGGILFICNLCLLYCYVKRRAGKHLFGGSSRG------SSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3725          

HSP 2 Score: 110.923 bits (276), Expect = 1.514e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847742|gb|GAXK01109802.1| (TSA: Calanus finmarchicus comp225879_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0
Identity = 379/919 (41.24%), Postives = 547/919 (59.52%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETL-SYLEGMVKPEDKDV--ERTISQGIILTITLVSGL-LLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+ T  +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T  S   G  +     V  ER     II+    V+G+ LLILNV+L+ C+++ R+   +  +     GS+ GS+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGT--FVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKSPPPGARQQMINSVLEERAEIPFIIIASVSVAGIFLLILNVVLLYCFIQNRKTENIKDNLSLG-GSSRGSSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3743          

HSP 2 Score: 110.538 bits (275), Expect = 2.004e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592847743|gb|GAXK01109801.1| (TSA: Calanus finmarchicus comp225879_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0
Identity = 379/919 (41.24%), Postives = 547/919 (59.52%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE-SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCK-SFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT---KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDV--HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETL-SYLEGMVKPEDKDV--ERTISQGIILTITLVSGL-LLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            MGQ V+L+C S+GGNPLA +VWY+ +++ID SY T+G +S+N   F+A  +DNN  YRC+AKNT+  EPLTA IV+SVQFAPD+V I GP  A VG TL+F+C T+NSNP + +QWV+D + AQ     RT+ S  GGW T SN+SV++G  D++K ISCYA N  L ET V+TH ++VLYPP + SITG   G I+ EG  RR+ C +++GNPL  L W+  D+ ++G   +  E+ T  +  SE+ +  + SDNG  Y+C+A + AT +   P   ++ L V F P  + V+ +PE P  G  A L C T SSNP   ++W  N  L   +  +   ++G FGG++T+++L IDV        + CEA +    +SV++   + V+ K  F++                     +E  S  + NI+ + NP  + Y W       IP  +L++  + R I +  + L++   + ED+G Y  +V NS GKSR  IK++V Y      IT + ++  GE+    C VD  P+  DTI W R G+D+  R +++  TN   L++ N + +DAG F+C ANNG+   +     +  FLLV  +P I++SP  SK+ASDK  T  L C+A G P+V F W R G  I+++     S   +          +D +TW+SEL +KN++ RDYG Y C A N  G S++ V L+ TS+PD P  L V + TYNSV LTW PGF+GG +Q F IR ++ GS+ + +VDV  H  +  +FEV DL++ T YSFS+ AFN LG+S +TSE V   T  S   G  +     V  ER     II+    V+G+ LLILNV+L+ C+++ R+   +  +     GS+ GS+KSATIEMY GSSYNET+SGETLSS+SEKS  Y +S ++
Sbjct: 1092 MGQAVTLVCESYGGNPLAAIVWYKNNQRIDHSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIREPLTAQIVMSVQFAPDRVEIRGPATAKVGQTLNFECATSNSNPASTLQWVIDGK-AQPAIHNRTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTVLYPPDELSITGYKQGDILQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGT--FVSSEISIKVDRSDNGKSYQCKAKSEATEE---PFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQ--TKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFID---------------APKEIDIEVNSSRIFNINAKGNPEILKYSWIKKGGTEIP--SLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPPRFVQITRSKLINEGESVKLLCQVDGFPLKTDTIKWERDGYDIASRATVETTTNGSVLSIQNATIKDAGIFICLANNGVPRGRPVEIREKLFLLVNHKPIIDDSPELSKSASDKGGTGKLICQADGAPNVTFKWFREGRAINEDNAAKYSVLDKS---------LDPLTWQSELKVKNIALRDYGHYGCAASNMWGTSQHQVILSATSKPDPPQRLRVLSSTYNSVILTWQPGFNGGFQQYFRIRLQRVGSEGFLFVDVFPHLPAATAFEVSDLQMDTAYSFSIMAFNKLGESAYTSELVQTRTAKSPPPGARQQMINSVLEERAEIPFIIIASVSVAGIFLLILNVVLLYCFIQNRKTENIKDNLSLG-GSSRGSSKSATIEMYVGSSYNETISGETLSSLSEKSDTYFTSDLE 3743          

HSP 2 Score: 110.538 bits (275), Expect = 2.005e-23
Identity = 160/749 (21.36%), Postives = 295/749 (39.39%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENF-TVRTESERGGWETRSNI--SVLIGPN--DRNKIISCYAINNDLGETVVQ-------THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGL--SIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNI-SVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSR----VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWES--ELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            G T  L+C    GN + +V W +    + +         Y   G +S   Y    S  S  ++ +Y C+        P+ A+  L+V   P ++ I      +  D      +   C  +N+ P A + W  ++ +       +   SE G    R      +   PN  D     +C A +  L  T  +       +  +SVLYPP KP I G   G  +  GQ+  L C S  GNPLA + WY+ ++ I         T + + +++ +   A   DN   YRCEA N    +   PL++ I +SV+F P  + ++  P   K G+     C T +SNP + + W  +     +I N    R  +   GG +T + + + V   D   +  C A N  L E+  +   +TV     L P  +         D       ++EGS   +   ++  NP  ++ +  A+   E       +S        + + ++I   R ++   Y+ K  +  +E   R  + + V +  T   +  +    + G  A  +C+ D+     + + W       G + + +     G+ +  L   +V++      + C A + +     + +T + V+ +P+  ++P       +  +  +    A+G P+++ +SW + G                 E   +  + + R    S   LH+ N+   D G+Y    +N  G SR ++ +NV   P
Sbjct:  429 GDTAVLLC--QVGNQIGQVQWTKDGLTLGYDRLLPGFDRYSVLGNDSLGTYNLQVSNASVQDDAKYECQVGPGRGNPPIRANAYLTVNLPPTQIQIMNHLSGSRIDIKENAEVEISCKVSNAKPRAGIIWYRNNVAFNPGTGNIEESSEDGTLPDRKTTFSKIKFRPNSADNQATYACEASHPALRGTAARPANPMRSSVLLSVLYPPEKPEIQGYLEGETIRMGQAVTLVCESYGGNPLAAIVWYKNNQRID-----HSYTTSGSKSKNTIMFLAQPDDNNAAYRCEAKNTMIRE---PLTAQIVMSVQFAPDRVEIRG-PATAKVGQTLNFECATSNSNPASTLQWVIDGKAQPAIHN----RTDTSAEGGWVTISNVSVMVGHLDKSKSISCYANNFALSETKVETHIVTV-----LYPPDELSITGYKQGDI------LQEGSLRRIKCTALSGNPLPELEWFAADKKVEGAKIEKAESGTF-----VSSEISIKVDRSDNGKSYQCKAKSEATEEPFRKSVALKVSFPPTRVKVEVEPESPIEGNPAVLKCLTDSSNPDVEILWWHNGELVAGSNSQTKAGKFGGSMTSSLLQIDVTEAHINSVYKCEARHAVTSKSVSNTTKIAVQYKPQFIDAPKEIDIEVNSSR--IFNINAKGNPEILKYSWIKKGGT---------------EIPSLNNSPLQRGIAASGHSLHLNNIQVEDSGTYIVRVENSIGKSRKSIKVNVEYPP 2531          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592929119|gb|GAXK01029426.1| (TSA: Calanus finmarchicus comp151307_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 576.63 bits (1485), Expect = 1.124e-179
Identity = 337/931 (36.20%), Postives = 522/931 (56.07%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISW-----------------SRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTY----QYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLI----LNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSS----YNETMSGETLSSISEKSGXYTS 903
            G TV+L+C+S GGNPLA + WYR  +++D SY T    S+N Y F A+ EDNN  Y C+AKN + V+PL A+I   VQ+ P  V I GP+EA  GD L+F+C T+NSNPP  + WVVD R+ Q N T +  SERGGW+T +NI+V + P DRN  I C+A+N ++ +T V++HTISVLYPP  P ITG   G ++  G+ RR+ C S+ GNPLAT+ WY G + I       +     NYA + L L+ N +DNG VYRC  ++   A+   P+  +I  +V+F P  + +  +P   + G+KA L CE+G +NP + ++W       + + G+   R G +GG  T + L +DV++RD G  + C+A N+  GE++ D +T+ +  K                     +   V EG   V+NIS  A+P  + Y+W      N+ + + DSD    ++    +L+I +  K  AG Y  + NNSEG +  KIK+DVL+   I++IT  +++ + + A FEC  DA P+  + I W                  R+  D +    +   T    L + NV+ +DAG+  C  +NG   ++    TFLLV+ +P ++ESPA  KAA+  R+T  L CRAQG+P++ F+W R    ++              KY++ + ++D +TWES+ H+ +++  DYG Y+C A+N  G+S   V L+  SRPDTP+ + + + + +SV L W+PGFDGG KQ+F ++Y    S +     + + V   +  SF +  LK  TKY FS+++ N++G+SE+++    +ET++   G   P    +  T SQ + + +T+  G+       L+V+LI+C V +RR  R    S +   S     K +  + ++GS     YN+TMS ET+S ISE+SG + S
Sbjct: 1265 GDTVTLVCISRGGNPLATITWYRNGQRVDSSYTTILNGSKNTYEFTATEEDNNALYSCQAKNDLIVKPLEAEITTIVQYGPKHVRISGPSEAMKGDELNFECSTSNSNPPVDIWWVVDGRAVQGNHTRKIASERGGWKTMTNITVRVDPTDRNINIQCHAVNQEVADTRVESHTISVLYPPEPPKITGYKEGDVLTGGKMRRIMCTSVGGNPLATIKWYLGSDEID-----SEYNVGDNYATATLDLSVNMTDNGAVYRCVVTSKVIAE---PMEKSIRTNVKFAPSFVNINVEPNTLRIGEKATLSCESGEANPPSVLVWLLKG--EVLSAGKQIFRKGNYGGKTTRSTLTVDVKSRDDGDVYTCKAYNDH-GEAL-DAVTLAIAYKPEF--------------PTVSSPVEVIEGEPFVLNISASASPTKLDYKWEKDMGGNL-DTSPDSD----VWFNGGILHIPKVSKNHAGTYTIRANNSEGMAVTKIKLDVLFSPKITNITQPLLLDSMDNAMFECTFDANPVMYEGIRWVRQEKESDEEVIVSGSSRRISVDWD---DISGNTVKSRLIIRNVTVQDAGKIACVFSNGYG-IEARGETFLLVKHKPIMDESPALKKAAAANRETGRLVCRAQGSPNITFTWRRQTGELA-----------GGSKYQVYHKMLDPLTWESQFHVLDINPADYGIYECAARNTMGVSYQMVMLDTPSRPDTPSEVMIVSSSSDSVKLKWVPGFDGGSKQTFRVKYYPATSISLYSQGRVIFVDPINETSFTITGLKSSTKYKFSIQSLNEIGESEYST---PIETMTE-AGNNPPLVDGLTDTSSQSVPIIVTISIGVCATLLLLLSVVLITCLVHKRRQKRTTSRSFSSDNS-----KPSVQDGFSGSQGTSCYNDTMSEETMSYISERSGSFIS 3892          

HSP 2 Score: 111.694 bits (278), Expect = 7.570e-24
Identity = 169/740 (22.84%), Postives = 281/740 (37.97%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--------------RNMYTFVASSEDNNKRYRCKAKN-TVSVEPLTADIVLSVQFAPDKVTIEG---PTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNY-AQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVK-CKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSR-VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANN--GIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV--FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGI-SRYAVYLNVTSRP---DTPTL 713
            + V LIC  H   P  +++WY GD++     +  GR                 ++  F  S+ DN++   C+A++  ++ EPL A I + VQ+ P +  IEG         GDT++  CI+   NP A + W  + +    ++T      +  +E  +         D N + SC A N+ + + +    T  V Y P    I+G  P   M +G      C +   NP   + W      +QG       +E   +   + + +  + +D  +  +C A N   A ++   S TI++    +P  I    + +V   GK   + C +   NP A + W      S E   E  V     G N  T  L + V   D+G  + C  T++ + E +   I   VK   SF+N       +P           S E G          ANP  V          +         N     + ++ L +    ++D   Y  K  N  G++   + + + Y      ++  V V+ GE         A P   D   W + +G +++         N   L +  VSK  AG +   ANN  G+A  K      L V   P+I           D    A+  C     P +     W R      +E +E+        +  + ++ I   T +S L I+NV+ +D G   C+  N  GI +R   +L V  +P   ++P L
Sbjct: 2135 EEVELICRIHNARPRPDIIWYLGDQE-----FIKGRREDSDDKGSLPERTTVTSILRFKPSAGDNSQNIACEAQHPALTTEPLRASIKMFVQYPPGQPQIEGYRNKQPLKKGDTVTLVCISRGGNPLATITWYRNGQRVDSSYTTILNGSKNTYEFTAT------EEDNNALYSCQAKNDLIVKPLEAEITTIVQYGPKHVRISG--PSEAM-KGDELNFECSTSNSNPPVDIWWVVDGRAVQGNHTRKIASERGGWKTMTNITVRVDPTDRNINIQCHAVNQEVA-DTRVESHTISVLYPPEPPKITGYKEGDVLTGGKMRRIMCTSVGGNPLATIKWYLG---SDEIDSEYNV-----GDNYATATLDLSVNMTDNGAVYRCVVTSKVIAEPMEKSIRTNVKFAPSFVN----INVEPNTLRIGEKATLSCESG---------EANPPSVLVWLLKGEVLSAGKQIFRKGNYGG-KTTRSTLTVDVKSRDDGDVYTCKAYNDHGEALDAVTLAIAYKPEFPTVSSPVEVIEGEPFVLNISASASPTKLD-YKWEKDMGGNLDTSPDSDVWFNGGILHIPKVSKNHAGTYTIRANNSEGMAVTK----IKLDVLFSPKITN--ITQPLLLDSMDNAMFECTFDANPVMYEGIRWVRQ----EKESDEEVIVSGSSRRISVDWDDISGNTVKSRLIIRNVTVQDAGKIACVFSNGYGIEARGETFLLVKHKPIMDESPAL 4210          

HSP 3 Score: 105.145 bits (261), Expect = 8.311e-22
Identity = 98/358 (27.37%), Postives = 164/358 (45.81%), Query Frame = 0
Query:   42 NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG---PTEANV--GDTLSFDCITTNSNP-PAIVQWVVDSRSAQENFTVRTES--ERGGWETRSNISVLI----GPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            N+    A+ ED +  Y C+     + +P+ A+  L+V   P K  + G    +E  V   + +   C   N+ P P I+ ++ D    QE    R E   ++G    R+ ++ ++       D ++ I+C A +  L  E +  +  + V YPPG+P I G      + +G +  L CIS  GNPLAT+TWYR      G   D   T   N +++    TA   DN  +Y C+A N    +   PL + IT  V++ P  + + S P     G +    C T +SNP  + IW   DG +++     ++ S   G    TN+  + V+  D  +   C A N+E+ ++  +  T++V
Sbjct: 3362 NLQIINATLEDESV-YECQVGPNGTNKPIRANAKLNVLLPPTKAELLGYKSDSELIVREEEEVELICRIHNARPRPDIIWYLGD----QEFIKGRREDSDDKGSLPERTTVTSILRFKPSAGDNSQNIACEAQHPALTTEPLRASIKMFVQYPPGQPQIEGYRNKQPLKKGDTVTLVCISRGGNPLATITWYR-----NGQRVDSSYTTILNGSKNTYEFTATEEDNNALYSCQAKNDLIVK---PLEAEITTIVQYGPKHVRI-SGPSEAMKGDELNFECSTSNSNPPVD-IWWVVDGRAVQGNHTRKIASERGGWKTMTNIT-VRVDPTDRNINIQCHAVNQEVADTRVESHTISV 4387          

HSP 4 Score: 40.817 bits (94), Expect = 3.101e-2
Identity = 84/352 (23.86%), Postives = 134/352 (38.07%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDE------QIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAI-VQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAI--NNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLA--TLTWYRGDE------IIQGXXXXXXX---XXXKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAG----KKAYLFCETGSSNPTANVIWRRNDG 327
            +G+  +L C S   NP + +VW    E      QI       G+ +R+  T    S D+   Y CKA N    E L A + L++ + P+  T+  P E   G+    + I+ +++P  +  +W  D         + T  +   W    N  +L  P         Y I  NN  G  V +   + VL+    P IT I    ++D   +    C +   NP+    + W R ++      I+ G+++         + N  +S L +      D G +  C  SN    +  G       L V+ KPI   +   P + KA     +   L C    S P     WRR  G
Sbjct: 2039 IGEKATLSCESGEANPPSVLVWLLKGEVLSAGKQIFRKGNYGGKTTRSTLTVDVKSRDDGDVYTCKAYNDHG-EALDA-VTLAIAYKPEFPTVSSPVEVIEGEPFVLN-ISASASPTKLDYKWEKDMGG-----NLDTSPDSDVW---FNGGILHIPKVSKNHAGTYTIRANNSEGMAVTKIK-LDVLF---SPKITNITQPLLLDSMDNAMFEC-TFDANPVMYEGIRWVRQEKESDEEVIVSGSSRRISVDWDDISGNTVKSRLIIRNVTVQDAGKI-ACVFSNGYGIEARG----ETFLLVKHKPI---MDESPALKKAAAANRETGRLVCRAQGS-PNITFTWRRQTG 3019          
BLAST of EMLSAG00000004534 vs. C. finmarchicus
Match: gi|592929120|gb|GAXK01029425.1| (TSA: Calanus finmarchicus comp151307_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 576.63 bits (1485), Expect = 2.068e-179
Identity = 337/931 (36.20%), Postives = 522/931 (56.07%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISW-----------------SRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTY----QYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLI----LNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSS----YNETMSGETLSSISEKSGXYTS 903
            G TV+L+C+S GGNPLA + WYR  +++D SY T    S+N Y F A+ EDNN  Y C+AKN + V+PL A+I   VQ+ P  V I GP+EA  GD L+F+C T+NSNPP  + WVVD R+ Q N T +  SERGGW+T +NI+V + P DRN  I C+A+N ++ +T V++HTISVLYPP  P ITG   G ++  G+ RR+ C S+ GNPLAT+ WY G + I       +     NYA + L L+ N +DNG VYRC  ++   A+   P+  +I  +V+F P  + +  +P   + G+KA L CE+G +NP + ++W       + + G+   R G +GG  T + L +DV++RD G  + C+A N+  GE++ D +T+ +  K                     +   V EG   V+NIS  A+P  + Y+W      N+ + + DSD    ++    +L+I +  K  AG Y  + NNSEG +  KIK+DVL+   I++IT  +++ + + A FEC  DA P+  + I W                  R+  D +    +   T    L + NV+ +DAG+  C  +NG   ++    TFLLV+ +P ++ESPA  KAA+  R+T  L CRAQG+P++ F+W R    ++              KY++ + ++D +TWES+ H+ +++  DYG Y+C A+N  G+S   V L+  SRPDTP+ + + + + +SV L W+PGFDGG KQ+F ++Y    S +     + + V   +  SF +  LK  TKY FS+++ N++G+SE+++    +ET++   G   P    +  T SQ + + +T+  G+       L+V+LI+C V +RR  R    S +   S     K +  + ++GS     YN+TMS ET+S ISE+SG + S
Sbjct: 1265 GDTVTLVCISRGGNPLATITWYRNGQRVDSSYTTILNGSKNTYEFTATEEDNNALYSCQAKNDLIVKPLEAEITTIVQYGPKHVRISGPSEAMKGDELNFECSTSNSNPPVDIWWVVDGRAVQGNHTRKIASERGGWKTMTNITVRVDPTDRNINIQCHAVNQEVADTRVESHTISVLYPPEPPKITGYKEGDVLTGGKMRRIMCTSVGGNPLATIKWYLGSDEID-----SEYNVGDNYATATLDLSVNMTDNGAVYRCVVTSKVIAE---PMEKSIRTNVKFAPSFVNINVEPNTLRIGEKATLSCESGEANPPSVLVWLLKG--EVLSAGKQIFRKGNYGGKTTRSTLTVDVKSRDDGDVYTCKAYNDH-GEAL-DAVTLAIAYKPEF--------------PTVSSPVEVIEGEPFVLNISASASPTKLDYKWEKDMGGNL-DTSPDSD----VWFNGGILHIPKVSKNHAGTYTIRANNSEGMAVTKIKLDVLFSPKITNITQPLLLDSMDNAMFECTFDANPVMYEGIRWVRQEKESDEEVIVSGSSRRISVDWD---DISGNTVKSRLIIRNVTVQDAGKIACVFSNGYG-IEARGETFLLVKHKPIMDESPALKKAAAANRETGRLVCRAQGSPNITFTWRRQTGELA-----------GGSKYQVYHKMLDPLTWESQFHVLDINPADYGIYECAARNTMGVSYQMVMLDTPSRPDTPSEVMIVSSSSDSVKLKWVPGFDGGSKQTFRVKYYPATSISLYSQGRVIFVDPINETSFTITGLKSSTKYKFSIQSLNEIGESEYST---PIETMTE-AGNNPPLVDGLTDTSSQSVPIIVTISIGVCATLLLLLSVVLITCLVHKRRQKRTTSRSFSSDNS-----KPSVQDGFSGSQGTSCYNDTMSEETMSYISERSGSFIS 3892          

HSP 2 Score: 112.079 bits (279), Expect = 6.848e-24
Identity = 169/740 (22.84%), Postives = 281/740 (37.97%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--------------RNMYTFVASSEDNNKRYRCKAKN-TVSVEPLTADIVLSVQFAPDKVTIEG---PTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNY-AQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVK-CKSFLNPLXXXXXXXXXXXXXXXXXXSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSR-VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANN--GIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV--FSWSRMGSVISQXXXXXXXXXXXXXXXXXXXNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGI-SRYAVYLNVTSRP---DTPTL 713
            + V LIC  H   P  +++WY GD++     +  GR                 ++  F  S+ DN++   C+A++  ++ EPL A I + VQ+ P +  IEG         GDT++  CI+   NP A + W  + +    ++T      +  +E  +         D N + SC A N+ + + +    T  V Y P    I+G  P   M +G      C +   NP   + W      +QG       +E   +   + + +  + +D  +  +C A N   A ++   S TI++    +P  I    + +V   GK   + C +   NP A + W      S E   E  V     G N  T  L + V   D+G  + C  T++ + E +   I   VK   SF+N       +P           S E G          ANP  V          +         N     + ++ L +    ++D   Y  K  N  G++   + + + Y      ++  V V+ GE         A P   D   W + +G +++         N   L +  VSK  AG +   ANN  G+A  K      L V   P+I           D    A+  C     P +     W R      +E +E+        +  + ++ I   T +S L I+NV+ +D G   C+  N  GI +R   +L V  +P   ++P L
Sbjct: 2135 EEVELICRIHNARPRPDIIWYLGDQE-----FIKGRREDSDDKGSLPERTTVTSILRFKPSAGDNSQNIACEAQHPALTTEPLRASIKMFVQYPPGQPQIEGYRNKQPLKKGDTVTLVCISRGGNPLATITWYRNGQRVDSSYTTILNGSKNTYEFTAT------EEDNNALYSCQAKNDLIVKPLEAEITTIVQYGPKHVRISG--PSEAM-KGDELNFECSTSNSNPPVDIWWVVDGRAVQGNHTRKIASERGGWKTMTNITVRVDPTDRNINIQCHAVNQEVA-DTRVESHTISVLYPPEPPKITGYKEGDVLTGGKMRRIMCTSVGGNPLATIKWYLG---SDEIDSEYNV-----GDNYATATLDLSVNMTDNGAVYRCVVTSKVIAEPMEKSIRTNVKFAPSFVN----INVEPNTLRIGEKATLSCESG---------EANPPSVLVWLLKGEVLSAGKQIFRKGNYGG-KTTRSTLTVDVKSRDDGDVYTCKAYNDHGEALDAVTLAIAYKPEFPTVSSPVEVIEGEPFVLNISASASPTKLD-YKWEKDMGGNLDTSPDSDVWFNGGILHIPKVSKNHAGTYTIRANNSEGMAVTK----IKLDVLFSPKITN--ITQPLLLDSMDNAMFECTFDANPVMYEGIRWVRQ----EKESDEEVIVSGSSRRISVDWDDISGNTVKSRLIIRNVTVQDAGKIACVFSNGYGIEARGETFLLVKHKPIMDESPAL 4210          

HSP 3 Score: 105.145 bits (261), Expect = 7.640e-22
Identity = 98/358 (27.37%), Postives = 164/358 (45.81%), Query Frame = 0
Query:   42 NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG---PTEANV--GDTLSFDCITTNSNP-PAIVQWVVDSRSAQENFTVRTES--ERGGWETRSNISVLI----GPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGXXXXXXXXXXKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            N+    A+ ED +  Y C+     + +P+ A+  L+V   P K  + G    +E  V   + +   C   N+ P P I+ ++ D    QE    R E   ++G    R+ ++ ++       D ++ I+C A +  L  E +  +  + V YPPG+P I G      + +G +  L CIS  GNPLAT+TWYR      G   D   T   N +++    TA   DN  +Y C+A N    +   PL + IT  V++ P  + + S P     G +    C T +SNP  + IW   DG +++     ++ S   G    TN+  + V+  D  +   C A N+E+ ++  +  T++V
Sbjct: 3362 NLQIINATLEDESV-YECQVGPNGTNKPIRANAKLNVLLPPTKAELLGYKSDSELIVREEEEVELICRIHNARPRPDIIWYLGD----QEFIKGRREDSDDKGSLPERTTVTSILRFKPSAGDNSQNIACEAQHPALTTEPLRASIKMFVQYPPGQPQIEGYRNKQPLKKGDTVTLVCISRGGNPLATITWYR-----NGQRVDSSYTTILNGSKNTYEFTATEEDNNALYSCQAKNDLIVK---PLEAEITTIVQYGPKHVRI-SGPSEAMKGDELNFECSTSNSNPPVD-IWWVVDGRAVQGNHTRKIASERGGWKTMTNIT-VRVDPTDRNINIQCHAVNQEVADTRVESHTISV 4387          

HSP 4 Score: 41.2022 bits (95), Expect = 2.980e-2
Identity = 84/352 (23.86%), Postives = 134/352 (38.07%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDE------QIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAI-VQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAI--NNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLA--TLTWYRGDE------IIQGXXXXXXX---XXXKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAG----KKAYLFCETGSSNPTANVIWRRNDG 327
            +G+  +L C S   NP + +VW    E      QI       G+ +R+  T    S D+   Y CKA N    E L A + L++ + P+  T+  P E   G+    + I+ +++P  +  +W  D         + T  +   W    N  +L  P         Y I  NN  G  V +   + VL+    P IT I    ++D   +    C +   NP+    + W R ++      I+ G+++         + N  +S L +      D G +  C  SN    +  G       L V+ KPI   +   P + KA     +   L C    S P     WRR  G
Sbjct: 2039 IGEKATLSCESGEANPPSVLVWLLKGEVLSAGKQIFRKGNYGGKTTRSTLTVDVKSRDDGDVYTCKAYNDHG-EALDA-VTLAIAYKPEFPTVSSPVEVIEGEPFVLN-ISASASPTKLDYKWEKDMGG-----NLDTSPDSDVW---FNGGILHIPKVSKNHAGTYTIRANNSEGMAVTKIK-LDVLF---SPKITNITQPLLLDSMDNAMFEC-TFDANPVMYEGIRWVRQEKESDEEVIVSGSSRRISVDWDDISGNTVKSRLIIRNVTVQDAGKI-ACVFSNGYGIEARG----ETFLLVKHKPI---MDESPALKKAAAANRETGRLVCRAQGS-PNITFTWRRQTG 3019          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000004534 (pep:novel supercontig:LSalAtl2s:LSalAtl2s237:371452:419819:1 gene:EMLSAG00000004534 transcript:EMLSAT00000004534 description:"maker-LSalAtl2s237-augustus-gene-4.26")

HSP 1 Score: 2371.27 bits (6144), Expect = 0.000e+0
Identity = 1144/1144 (100.00%), Postives = 1144/1144 (100.00%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVDPIHGHHHHIHHHPHPLPHNVPPSGGAXTGAPKNNGGHNTHHHHTLPSSTSHNHHHHHQSNRRSHRREEESMIDKSVCSDHRHQHHHHHHLDQPTVMGAASTYLVDNPPSRASSNHLNYVTMPRRAPHPRQSSTPSEPLMVPRESSIEDQGRQAYIQSLGIDNDGDDVVGVGRGIYASTLDIESPIKKTYNGLYVPPADYEYSDSISLPPPPSRDSDYTRPHFGSLRHYSPETAGHLV 1144
            MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVDPIHGHHHHIHHHPHPLPHNVPPSGGAXTGAPKNNGGHNTHHHHTLPSSTSHNHHHHHQSNRRSHRREEESMIDKSVCSDHRHQHHHHHHLDQPTVMGAASTYLVDNPPSRASSNHLNYVTMPRRAPHPRQSSTPSEPLMVPRESSIEDQGRQAYIQSLGIDNDGDDVVGVGRGIYASTLDIESPIKKTYNGLYVPPADYEYSDSISLPPPPSRDSDYTRPHFGSLRHYSPETAGHLV
Sbjct:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVDPIHGHHHHIHHHPHPLPHNVPPSGGAXTGAPKNNGGHNTHHHHTLPSSTSHNHHHHHQSNRRSHRREEESMIDKSVCSDHRHQHHHHHHLDQPTVMGAASTYLVDNPPSRASSNHLNYVTMPRRAPHPRQSSTPSEPLMVPRESSIEDQGRQAYIQSLGIDNDGDDVVGVGRGIYASTLDIESPIKKTYNGLYVPPADYEYSDSISLPPPPSRDSDYTRPHFGSLRHYSPETAGHLV 1144          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000000795 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1133:22645:64219:1 gene:EMLSAG00000000795 transcript:EMLSAT00000000795 description:"maker-LSalAtl2s1133-augustus-gene-0.5")

HSP 1 Score: 567 bits (1460), Expect = 4.636e-179
Identity = 317/822 (38.56%), Postives = 463/822 (56.33%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRS--AQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRS----GPFGGNLTTNVLGIDVEARDH-GVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQN-LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGT------------NSMHLTVYNVSKEDAGEFLCTANNGIADMKDT--KSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET 800
            + Q+V+LIC S GGNPLA+V+WYR  E  D SY ++GRESRN +TF+A ++D+N R+RC+A N  S EP+ A+IVLSVQFAP K+ + GP EA   D++   C T NSNPPAI+QW++D+    A   FT +  S  GGW T SNISV    N +NKI+SC+ +N++L +   ++  +SV+YPPG P+I G+    ++  G+ +RL C SMAGNP  TL WYRG E++   T   D+     +A +EL       DN    RCEA N A      P+ ++  LSV F P  + ++S PE P +G+K+ L CE+G+S P + + W   D   I+      VRS    G FGG  + + L + V  R H G    C A N    + V+D IT++V              KPIF ++ T   F   EG + ++N++  ANP   TY+W+   N        D +   +  +IQ+ +L I +A +  AG Y     N  G S +  +ID+LY   I  +TD+V+V  GE A FEC VDA P    TI W R  +DMEGRT    G+             ++ LTV N +  D+G F C A+NGI          T+LLVR +P ++++P N KA ++  +  VL C A G P+V  SW R  S       ED+      EKY I + ++D +TW SE +I +V  +DYG+Y+CIA+N EG +R  +  +V ++PD P+ L V    + SV+L W+PGF+GG  Q F +++  +     ++ DV   + ++F V  L+ G +Y+F++ AFN+ G+S +T  +V V T
Sbjct:  253 VAQSVTLICTSRGGNPLADVIWYRNGELADKSYTSSGRESRNTFTFIAETDDDNARFRCEASNEFSAEPMDAEIVLSVQFAPSKIYLYGPNEARRDDSVDXSCSTXNSNPPAIIQWIIDNSXLDASSYFTQKLPSPMGGWITVSNISVKXEENKKNKIVSCHVLNSELNDVKTKSQILSVIYPPGTPTIQGLSFDDLLIAGKLKRLKCTSMAGNPPPTLRWYRGKELLHSRTVITDQ-----FASAELEFIPARKDNLGDLRCEAINSAL---RFPVYASQNLSVDFSPNFVKIESHPEFPVSGEKSLLTCESGTSQPPSQITWWIGDEEIIDG-----VRSNDYPGEFGGKRSRSELQLLVSGRIHNGALIRCRAQNHL--DYVDDTITLSVA------------HKPIFTVEDTTAPFEXIEGKEAIINMTALANPPVSTYKWSLNLN--------DEEQNRQGLTIQDGVLKINKASRSHAGIYAVSATNXRGTSSITFEIDILYAPKIVYLTDSVLVGEGEPALFECAVDANPFNIQTIHWFRENYDMEGRTKTTQGSVVDKSGSGGEHVGTVMLTVLNATASDSGAFWCEADNGIERRGRIARNRTYLLVRHKPWVKQTPNNIKAXANPGEMGVLICEASGVPNVTISWVRSSSAF-----EDK------EKYRIVHTMLDPLTWRSEFYIHDVGIKDYGTYECIARNTEGSARQKIVFDVKTKPDIPSKLRVDKILHESVHLMWVPGFNGGTDQHFRLQFSSQ-ENGVEFRDVXPVNADNFVVNKLEPGVEYAFNIMAFNNQGESNYTDSSVIVHT 1027          

HSP 2 Score: 106.301 bits (264), Expect = 2.576e-23
Identity = 98/401 (24.44%), Postives = 161/401 (40.15%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTF--VASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV-----GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTES--------ERGGWETRSNISVLIGPNDRNKII--SCYAINNDLGETVVQTHTI-SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGE 376
            GQ+ ++ C    GN    V W R    + F         Y  +G+E    +    V ++ +++  + C+        P+ A   L V   P ++ I G    +       + +   C+   + P A + W       +EN    TES        E       S  S+ I P  ++  +  +C A++  L    ++   + SV +PPG+P ITG   G  +   QS  L C S  GNPLA + WYR  E+      D   T +   +++     A   D+   +RCEASN  +A+   P+ + I LSV+F P  I +   P   +        C T +SNP A + W  ++     +    +    P GG +T + + +  E         C   N EL +
Sbjct:   39 GQSTTIPC--EIGNRHGSVQWTRDGLTLGFDRKLPGFSRYSVSGKEINGQFNLEIVNATLNDDATFECQVGPADGNSPIRARAKLDVLLPPKRIEITGLDSGSRIIIRENEEVELKCVVFEAKPKATIIWY------RENSKFFTESRIDEDTIGEDAEHRVTSISSIRIRPTQKDNHVTWACEAVHPALKNAPMRASVLLSVQHPPGRPEITGYIEGETIRVAQSVTLICTSRGGNPLADVIWYRNGEL-----ADKSYTSSGRESRNTFTFIAETDDDNARFRCEASNEFSAE---PMDAEIVLSVQFAPSKIYLYG-PNEARRDDSVDXSCSTXNSNPPAIIQWIIDNSXLDASSYFTQKLPSPMGGWITVSNISVKXEENKKNKIVSCHVLNSELND 422          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000004704 (pep:novel supercontig:LSalAtl2s:LSalAtl2s247:383895:431889:-1 gene:EMLSAG00000004704 transcript:EMLSAT00000004704 description:"maker-LSalAtl2s247-augustus-gene-3.26")

HSP 1 Score: 266.544 bits (680), Expect = 2.401e-73
Identity = 230/810 (28.40%), Postives = 359/810 (44.32%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID-------FSYYTTGRE-----SRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVV---DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDL-GETV--VQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQ-----SELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSD-PEVPKA-----------------------------GKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGK--SRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSR-----VGF----DMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRM--GSVISQEP-EEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGS-YDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFII 742
            G+ ++L C+S GGNPL ++ W RG  +I+        S  +T +      S+++  FVA+  +NN  + C                                       L+F+C   N NP   ++W +   D      N   + ++ +GG+   S ISV I   D  +I ++C AI+    GE +  +   +I+VL PP +P I+G+     + EG+  +L C S  G+P   + WY GD          +      YA+     S ++LTA+  DN  +YRC A N A   +  P S+TITL V F P ++ +  + P+ PK+                             G+   + C++ SS P A + W  N GL  ++Q  +   SG FGG  T +V+       DHG  F C A N  + E      T  VK    LN L      P FD +  +T +S+ EG    +++S   NP ++ Y W  P N         + N  R       L I  A + DAGRY+   +NS G   +   + ++V Y  +I ++T    +    T  F C VDA PI+   I+W+      +G      M   + M N T++  LT+ +  + + G   C A N I+       T+L V  +P I +SP  +K A  K   A LTCR+ G P+V F W +   G  ++  P +E + E ++ +K    +    R TW+S L + +V    Y + + CIA N+ G   + V L    +PD P  +      +NSV LTW PGFDGG+ Q++ +
Sbjct:  207 GEIITLTCLSRGGNPLPDLYWERGPRKINPISMPQLKSIPSTDKNKKEGTSQSILKFVATPRENNISFSCSR-----------------------------------SLLNFNCTAFNGNPAPRIEWSLNGDDYVGXSINNHTKLDNRKGGYIFTSQISVHILETDAEEISLACSAISRSYEGEIMKEIDEISITVLKPPSQPRISGLPVSKSLREGERVQLNCSSPDGSPRPRVDWYLGD--------SPNVIPESVYAKVPIPHSMINLTASRRDNERIYRCSARNDANVHK--PSSTTITLQVFFPPDSVIINVERPQSPKSNIVQSNAVNPYYNNIGFRSRFSPKTTVLEGEFLRVTCQSSSSKPRATIQWHVN-GLPHKSQS-VSFPSGEFGGTRTVSVIEFKASYMDHGQVFECFAKNIAISE------TRIVKTNLTLNVLY----APKFDSNIQET-YSMVEGETRNISLSAVGNPSEIKYVWDFPSN---------NFNRSRFIIDGPFLIIQNANRRDAGRYRISASNSYGNFTTSKSVMVNVQYAPSIVELTPNKTIEELSTVEFHCKVDANPISDQLITWTYEEQHPLGLLKVSTMRTNSHMSNSTST--LTIISAHRGNTGYITCHARNNISKTAIHNRTYLTVNHKPWIFKSPTLNKFAIPKGDPAELTCRSTGVPEVHFKWEKTYNGRTVTVLPIDEYKGETVKPKKEYTGF----RFTWDSVLRLDHVRPEYYSTIFRCIASNKFGEDIHTVELVPRGKPDPPINVRFLASNHNSVTLTWDPGFDGGYNQTYFV 943          

HSP 2 Score: 60.8474 bits (146), Expect = 2.041e-9
Identity = 49/201 (24.38%), Postives = 80/201 (39.80%), Query Frame = 0
Query:   79 QFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDS---RSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-----GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDD-------DETETKNYAQSELHLTANYSDNGMVYRCEAS 264
            +  P +V  E P     G+     C +++SNP   + W  +    ++A+    +      G + T S I + +   D    I C A    +      E       ++VLYPP +  I   D   +  +G+   LTC+S  GNPL  L W RG   I   +          D+ + +  +QS L   A   +N + + C  S
Sbjct:   79 KVPPKRVKFEKPKSVVAGNQEIIACTSSSSNPATKLLWYKNEELIQNAELEEEILLGDRPGTYTTVSRIKIRLVKEDNGVFIICVAXQPAIFSRSDREYFQYKSKLNVLYPPERGPIILGDLRPMRTKGEIITLTCLSRGGNPLPDLYWERGPRKINPISMPQLKSIPSTDKNKKEGTSQSILKFVATPRENNISFSCSRS 279          

HSP 3 Score: 53.1434 bits (126), Expect = 4.851e-7
Identity = 51/197 (25.89%), Postives = 88/197 (44.67%), Query Frame = 0
Query:  707 RPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRY--RKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDS----EFTSENVAVE-TLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGST-----NGSTKSATIEMYAGSSYNETMSGETL 891
            +PD P  +      +NSV LTW PGFDGG+ Q++ +     + G+     ++V++   N   +  L+  T YSF + A N  G S    E   +  A+E +L   +G+           +S+  I+ I +   +  ++ ++ ++C++K+R+  R    S                KS  IE Y  S Y+  MS E  
Sbjct:  966 KPDPPINVRFLASNHNSVTLTWDPGFDGGYNQTYFVTVIEERTGNIVGHLLNVNNIQGNVTTIIGLRPRTPYSFKITAKNKAGISLPGGELIKKTEAMEHSLPVNDGI-----------LSRVAIIGIIIGVIIAFMIFLVFMTCFIKQRKRRRTRRRSSTSSRRNSSSKDKSDNKSVMIERYTPSKYSSAMSDELF 1151          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000011552 (pep:novel supercontig:LSalAtl2s:LSalAtl2s7:1384693:1561256:-1 gene:EMLSAG00000011552 transcript:EMLSAT00000011552 description:"maker-LSalAtl2s7-augustus-gene-15.10")

HSP 1 Score: 99.3673 bits (246), Expect = 1.981e-21
Identity = 83/294 (28.23%), Postives = 131/294 (44.56%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES-RNMYTFVASSEDN-NKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTE-ANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-GETVVQTHTISVLYPPGKPSITG--IDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPI 288
            +G TV L CV++GG P  EV W+R    ID SY  +  ++ +N  T +A  +D+  +++RC+A N     P +  I L + F P  V I  P E  +        C++  S P A + W  ++     +F  RTE+   G +T S+ +    PND  K ++C A N  L G  +     +++ Y P      G  ++P  I  EG      C   +  P+  + W   ++I+             +    E+   A  S     Y C ASN     E   +S+TITL V +KP+
Sbjct:  145 VGSTVILKCVTNGGRPSPEVTWWRDHSLIDSSYEKSFSQTVKNTLTLLAIKKDDLGRKFRCQASNNNVSLPASTSITLDLLFRPTSVRIVTPKEPLSTSKVYKIKCMSLGSRPSATITWWRNN-----DFLGRTETWEEGNKTYSSFTYTPKPNDNGKRLTCRAENTKLKGSAIEDVWKLTIYYIPEIILNFGANLNPNNIA-EGNDVYFECNISSNPPVYKIVWRHNNKIVNHNKSKGIIISGTSLVLQEVKRNATGS-----YSCTASN----TEGDIISNTITLRVMYKPV 423          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000006645 (pep:novel supercontig:LSalAtl2s:LSalAtl2s367:2485:101243:-1 gene:EMLSAG00000006645 transcript:EMLSAT00000006645 description:"augustus_masked-LSalAtl2s367-processed-gene-1.0")

HSP 1 Score: 95.1301 bits (235), Expect = 3.446e-20
Identity = 113/437 (25.86%), Postives = 185/437 (42.33%), Query Frame = 0
Query:  169 TVVQTHTISVLYPPGKPSI-----------TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRN-DGLSIENQGEIRVRSGPFGGNLTTN---VLGIDVEARDHGVTFICEATNEEL---GESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEG-KSRLKIKIDVLY-GATISDITDAVMVVTGETAHFECVVDAKPITADTISW----SRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            T+    T+ ++ PP +P I            GI P     EG S  L C    G PL ++ WY  D ++  T Q   E   KN   +   L   ++++  + RC ASN    +   P  S + L + F P  + ++   +   +G+  Y+ C    SNP AN+ W R   GLS         ++    GN++ +    L +    RD     IC  +N+EL    + V D   + +    F  P +      +  ++P D    +EEG D+  + ++ ANP      W +   E   +I     N   I + Q+L+ +   R++ AG Y  + +N EG +    + I ++Y    + D      V   ETAH  C VDA P  A++ SW    S  G ++   +    G+ S+ L+    ++ D G  LC A N + 
Sbjct:  112 TIYHHITLDIIVPPQQPLILDDRGREVKGTMGIGP---FREGDSLTLECHVPGGEPLPSVLWYMDDVLMDDTFQQTYEGTVKN-GLTIRKLERKHTES--LLRCLASNNNITR---PKESHVQLKMFFPPQGVKIQGPLQPYTSGRVYYISCVVWGSNPPANIKWYRGIKGLSDLVPLSSYNQTVTHSGNVSLSWIKYLPLPSHQRD---LLICRGSNQELMGHQQFVEDFHRLEI----FYAPNVMLTLGRM--VNPKD----LEEGDDVYFSCNIDANPAAYKLTWRHNGEEVTQDI-----NKGIIIANQSLV-LQRIRRDQAGSYSCEASNVEGDEVSSPVAISIMYRPVCVKDQKTIYGVSEHETAHISCKVDAXP-QAESFSWSFNTSSGGMNLPRNSFTNKGSGSL-LSYTPQTQMDYGTVLCWAENMVG 518          

HSP 2 Score: 83.1889 bits (204), Expect = 1.792e-16
Identity = 80/337 (23.74%), Postives = 138/337 (40.95%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT--GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA-NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESE---RGGWETRSNISVLIGPNDRNKIISCYAINNDL---GETVVQTHTISVLYPPGKPSITG--IDPGTIMDEGQSRRLTCISMAGNPLA-TLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRN 325
            G +++L C   GG PL  V+WY  D  +D ++  T  G     +       +      RC A N     P  + + L + F P  V I+GP +    G      C+   SNPPA ++W    +   +   + + ++     G  + S I  L  P+ +  ++ C   N +L    + V   H + + Y P      G  ++P   ++EG     +C ++  NP A  LTW + G+E+ Q      D  +    A   L L     D    Y CEASN     E   +SS + +S+ ++P+ +  +         + A++ C+  +     +  W  N
Sbjct:  150 GDSLTLECHVPGGEPLPSVLWYMDDVLMDDTFQQTYEGTVKNGLTIRKLERKHTESLLRCLASNNNITRPKESHVQLKMFFPPQGVKIQGPLQPYTSGRVYYISCVVWGSNPPANIKWYRGIKGLSDLVPLSSYNQTVTHSGNVSLSWIKYLPLPSHQRDLLICRGSNQELMGHQQFVEDFHRLEIFYAPNVMLTLGRMVNPKD-LEEGDDVYFSC-NIDANPAAYKLTWRHNGEEVTQ------DINKGIIIANQSLVLQRIRRDQAGSYSCEASN----VEGDEVSSPVAISIMYRPVCVKDQKTIYGVSEHETAHISCKVDAXPQAESFSWSFN 474          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000005066 (pep:novel supercontig:LSalAtl2s:LSalAtl2s266:205294:402426:1 gene:EMLSAG00000005066 transcript:EMLSAT00000005066 description:"maker-LSalAtl2s266-augustus-gene-3.8")

HSP 1 Score: 92.8189 bits (229), Expect = 3.120e-19
Identity = 153/663 (23.08%), Postives = 248/663 (37.41%), Query Frame = 0
Query:  173 THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSD-NGMVYRCEASNPATAQESGPLSSTITL---SVRFKPI-TIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMH----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQR----PRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV----------------TSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHK--QSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYL 804
            T  IS  Y    P IT       + + +   L C    G P   +TWY+  E++  T          + +   L    N  + +G +Y CEA+N      S  ++  + +     R  P+ TI  + D  V K        C     NP  +V W +N  +   +Q   R+     G     N++  +V   D G    C+A N  +G    +   ++++ K +      F+ KP+      D      +  D++ ++S   +P  +  R   P  EN   +++D+          N L I + R ED G Y  K  N  G       I V     I        +     A FEC     P    ++ W++ G         + G  S+     L +  V KED G ++C+A + +A    TK+   +   R    P I    AN     +    A+L C+A G P    SW +  S IS     D+S  I  E                 L I N+ S D G Y C A +E G + ++  L+                 ++ PD PT   + +    S+ ++W    + G      + + Y     +T   V  +  ++ ++ + +LK  T Y F V+A N  G S  T  +  V TL  L
Sbjct:   19 TSVISAQY--RSPQITEQPTSMTVPKNEPVTLNC-KADGIPEPNITWYKDGELVP-TAPKTHRVILPSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGDIGVIK--------CWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVG-----NLVIQEVVKSDEG-QXACQAENI-VGIRETEEAQLSIQVKPY------FLSKPL------DITAISGDDIDIICDVSGDPDPIVIWSRDDGPLPEN--KVSIDA----------NRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHAKPAIQTKPKDQRIGMNGIAKFECSAGGNP--PPSLFWTKEGSQELMFAGTKYGQRSVSEDGTLFIQGVRKEDEGFYICSALS-VAGSTATKAYLEVTALRDTPPPIIIMGTANQTLPLNT--IAILPCKASGAPKPTISWYKNQSPISI----DDSSRILVES-------------SGTLVIDNLISSDTGLYTCTASSESGETSWSASLSXXDPKNPNVIFHKTPDPSTFPDPPTRPKIVDRRSTSITISWRRSPNTGESPLMGYTLEYFSSDLETGWVVAANRITSETYTINNLKPDTSYMFLVRAENSHGMSPPTRVSERVRTLQSL 616          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000000256 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1038:13532:133097:1 gene:EMLSAG00000000256 transcript:EMLSAT00000000256 description:"maker-LSalAtl2s1038-augustus-gene-0.5")

HSP 1 Score: 90.5077 bits (223), Expect = 1.575e-18
Identity = 70/283 (24.73%), Postives = 124/283 (43.82%), Query Frame = 0
Query:  521 FECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG--ISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDG--GHKQSFIIRYRKKGSKTYQYVD--VHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFT 792
             +C ++A P     ++WS+       FD++G  + QNG+    L + +V++  AGE++CT  N       +    +L++  P +   P      S   K   + C A G P     W ++   +  +  +                      +  EL I+N+   D+G Y+C+  NE     ++  +Y+  T+ P  PT + +      +V + W+PG+ G    KQS+ IRYR+K SK   +++  V        ++ +L   TKY F V   N+ GD  F+
Sbjct:   63 IQCHIEANP-EIQFVTWSKDKRVYDPFDVDGXFNAQNGS----LIIESVNQRQAGEYVCTPYNIHGTAGPSGVMQVLIKDPPTLMRRPEKLYHRSVGGK-VTMPCGAIGVPHPDIKWRKLRGRLPHKRSK---------------------VYNGELTIENLRKSDHGVYECVVSNEVATITAKAELYIEKTT-PHAPTNITITETEVFAVTIQWLPGYSGCASCKQSYKIRYREKDSKFPNWIELPVXPPDARKIQIHNLSALTKYEFQVIGTNEYGDGMFS 317          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000011914 (pep:novel supercontig:LSalAtl2s:LSalAtl2s840:4759:167747:-1 gene:EMLSAG00000011914 transcript:EMLSAT00000011914 description:"maker-LSalAtl2s840-augustus-gene-0.12")

HSP 1 Score: 88.9669 bits (219), Expect = 4.815e-18
Identity = 185/841 (22.00%), Postives = 324/841 (38.53%), Query Frame = 0
Query:   10 VSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNT-VSVEPLTADIV-LSVQFAPDKVTIEGPTE-ANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISV---LYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLA-TLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQG-EIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVN-DGI------TMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGA--TISDITDAVMVVTGE-TAHFECVVDAKPITADTISWSRVG-------------FDMEGRTSMQNGTNSMHLTVYNVSK---------------EDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEI----EDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQS-FIIRYRKKGS-KTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAV 798
            V+ G +PL E   Y  D +I+   Y       ++    AS   +N ++ C+ K   +S   L +  V L+V   P   +I+  +  A  G+ L+  C +   +P   + W  +  +      +   +++    T S +S++   +D      C   N  LG+    T++I V    +P  K  +   +P  I+ +G + +LTC  +   PL   + W + D+I+  TT  D +    N   S  ++    +DNG+                 +   I +SV F P+ I +    E+ +  +   + C   ++     + W + +  +    G ++++R+               +   D G+ ++C ATN     S+  D I      TM +  +      I         I P + I  VE    L    +    P      W +  N+NI +I    +     +++  +L   E      GRY  + +N  G          +Y A   I+ +   ++   G+      C    KP     + W + G             +  E     + GTN     VY+V K                D G+F+C   N I  ++   ST L ++  P +      +K A+D      + C  +  P     WS    ++ +   E     +    EDE             +   L + +VS  DYG Y C A N  G  R  + L   S+PDTP    + +  +NSV L W  GF+GG   + FI++Y K      ++Y      S N   + +L   T+Y F VKA N +G+S +++E +A+
Sbjct:   88 VAIGPSPLQEN--YSVDXRIESGIY-------DLRIINASYNRDNGKFECRIKEAGLSFNELYSKSVELTVLLKPSTPSIQMSSPIATEGEQLNLTCSSIGGSPTPQITWFNEGGTQSLEAEIIKGNDKDD-PTYSILSIIPRKSDDQTSYFCSVWNRALGQIQKLTNSIKVNVNYFPRIK--VGPHNPYRII-KGGTAKLTC-RVDSKPLVHKVRWMKRDQIL--TTAFDYDIGRANIQHSGSYMCL--ADNGL--------------GRXVHEEIKVSVLFXPV-ITLPDSREMNR-NEDIVVDCMVRANPSPIKIEWFKENDHNFHQPGNKLKIRA---------------LSPYDAGI-YVCSATNHMDPSSIAPDKIVRISNGTMQIHIRHRPGNAI---------IMPRNPIGVVERSLTLRCEANPPGFPLPQYSWWKDEGNQNITSIFGGQE-----FTLHQVLLSHE------GRYCCQSHNDLGDGSFSCVTLKVYEAPKMIARLQPKLIRKEGDLNFQISCGAIGKP--KPVVRWFKDGKLIRDSESKVYQIYTSEQPVDAERGTN-----VYSVLKFVGPQRMXPANRLLPPDKGQFVCRFENEIDRVES--STELKIKHAPIMINQ--FNKIAADLAHKIYIRCSVRAFPTPRIEWSFRQRLLRESAYEMNMHPVGPGKEDE-------------YTGYLKVPDVSPGDYGDYSCTAINSMGSKRSNIKLQKKSQPDTPESPWIIDVGFNSVKLGWREGFNGGFNDTLFIVQYNKHDDLLLFRYQKC--VSQNPCNITNLYQNTRYFFKVKAANLMGESNYSNEVMAI 832          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000005454 (pep:novel supercontig:LSalAtl2s:LSalAtl2s293:185286:259880:1 gene:EMLSAG00000005454 transcript:EMLSAT00000005454 description:"maker-LSalAtl2s293-augustus-gene-2.25")

HSP 1 Score: 82.0333 bits (201), Expect = 7.404e-16
Identity = 128/523 (24.47%), Postives = 210/523 (40.15%), Query Frame = 0
Query:  196 MDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPN---ITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDM---EGRTSMQNGTNSMHLTVYNVS-KEDAGEFLCTANN--GIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQ-GTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
              EG S  L C +MA +P+ +  WY+  E          +   K    + +   A   D+G  Y C  +N    +    + + +T++    P+++ V+   +V   G+     C     NP   V W +N      N+  +R+ S               V+  D G+ + C   NE+      +    T + K     L    D P F     +TI  V+ G  L ++   + +P     +W   Y + + N   IT+ S        + + LNI++ + +  G Y+ + ++  GK     K++V +GA        + VV G++    C V   PI+   I W + G  +   + +T   NGT    L + ++  KEDA  + C A N  G +   D + +   V +RP I      S +  D+   A ++C  Q G   +  SWS  G  +S EP    S+            V  R    S L I +V  R  G+Y C AQN+ GI  Y+  L V   P
Sbjct:  102 FKEGLSFALICPAMA-SPIPSFRWYKFMEANNNKKLVKLDDRVKXVGGTLIIKEAKVEDSGK-YLCMVNNSVGGES---VETVLTVTA---PLSVDVEPKTQVVDYGRSVTFKCNY-KGNPIKEVFWLKNGKSINHNENTLRINS---------------VKKDDRGM-YQCFIRNEQ------ESAQATAELK-----LGGRFDPPEFISHFEETI--VKPGGYLSISCIAKGDP-SPNIKWFI-YGKEVKNEGAITVGSFRDAN-GDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNV-FGAPYVKKMRPMKVVAGKSMFITCPVAGYPIS--NIVWEKDGRQLPFNDRQTVFPNGT----LIISDIQRKEDASVYTCVAXNDEGYSARSDLEVS---VMERPEIVPFFFGSDSV-DEGLYAQVSCIVQRGDTPIKISWSLKGDTVSSEPGMTTSQ------------VGPR---ASILSIHSVGYRHSGTYTCTAQNKAGIISYSTSLTVNEPP 557          

HSP 2 Score: 81.2629 bits (199), Expect = 1.491e-15
Identity = 146/637 (22.92%), Postives = 242/637 (37.99%), Query Frame = 0
Query:  195 IMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKP--IFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRW-ANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSR--LKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRV-----------------GFDMEGRTSMQ--NGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIE-ESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQS-FIIRYRKKGSKTYQYVDVHH----SSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET 800
            +M+EG   +++C+  +G+    +TW + G+ I  GT      T            + NYS  G  Y C+A N A     G  + T  L V   P+        EV   G  A L C   S +   +V W  +        G      GP    L  N +G       H   + C A+N     + +D  T  ++           +++P  +  ++    +FS    + +V  I+    P  +++ +      ++  NI +   NI    SI   L I     +  G Y    +N  G S    ++KI+V    T+ + TD      G  A  +C  D  P     I W +                    D+    +M+  +GT    L + N+ K + G +LC A+NGI  +       + V+  P  E +    +   SD     VL C A+G   +   W+     +  +          D++Y I+  VID     S + IK     D   + C+A N  G    ++ L +   P+TP  L V +    +V L+W   ++G    + ++I +  K +K     D+ H       N   V  LK    Y F + A N +G S   S+ + +ET
Sbjct: 1823 VMNEGDFAQISCVITSGDLPLLITWSFHGNTI--GTNTGIATTNLGPRMSVLAINSVNYSHQGK-YTCQAKNKA-----GIRTHTAELKVNEPPMIGPFAFPKEVMNEGNFAQLTCVVTSGDLPLSVTWTFHGDKVGTQTGITTTNLGPRLSLLVINSVGYR-----HRGNYTCXASNN----AGHDSYTAKLR-----------VNEPPSLVPLNFGKNVFSEGSFAQVVCTITEGDEPIKISWSFHGESLEDSGQNIGIAITNIGSRTSI---LIINSVEHKHRGNYTCSASNKAGNSSDTAQLKINVRPRWTV-EPTDK-HFAQGSEAKIDCKSDGFP--QPKIVWRKAIGLPVSNEQXPTPSDYKDLDVSNDRNMKIIDGT----LIIKNIQKSNEGFYLCKASNGIGGISAVAK--ISVQAPPSFEIKKRTQTALISDN---TVLQCEAKGEKPIGVLWNMNNKRLDAK---------VDDRYTIREEVIDDGVL-STISIKRTERGDSALFTCVATNAFGSDDTSINLIIQENPETPYDLKVLDKAGRTVKLSWQTPYNGNSNLTRYVIEF--KPTKGSWKNDIDHVLVPGDQNQAVVYSLKPANSYHFRIVAQNVIGVSG-PSDTITIET 2402          

HSP 3 Score: 77.7962 bits (190), Expect = 1.556e-14
Identity = 156/706 (22.10%), Postives = 259/706 (36.69%), Query Frame = 0
Query:   57 YRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-GPTEANVGDTLSFDCITTNSNPPAIVQWVV--DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEG--SDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDIT-DAVMVVTGETAHFECVVDA--KPITADTISWSRVGFDMEGRTSMQN---GTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVY---LTWIPGFDGGHKQSFIIRYRKKG 748
            Y C A+N   +  ++    L+V   P  + +  G    + G+     CI +  + P  + W +  D  S++   +           TR++I  +     R+  +      N+ G    Q+  + V  PP    +T  D   +++EG+  +L CI   G+   TLTW    ++I           +     S   L   Y  +G  Y C  +N A     G  S +  L V   P  + +    EV   G+ A + C     +   ++ W                  G       T++L I      H   + C+ATN+    S +    + V     + PL     KP           S++EG  + +V  +S    P  +T+        + P+I+ +      + S  ++L I        G Y  +  N  G  R    + V    +I   +  A  V  G+     C+V+   +P+T   I+WS  G  +    S+     GT +  L + NV    +G + C A         T S  L V + P I      S    +   T +    ++G   V  +WS  G VIS EP              I   +    T  S L I NV  R  G Y C A N  G + ++  LNV    ++P++L  F++  +SV    +T +  F     +   I +  KG
Sbjct:  534 YTCTAQNKAGI--ISYSTSLTVNEPPSLMPLTFGRDILDEGEFAQLICIVSKGDEPLTMSWTLKGDDVSSEPGLSTTKIG------TRTSILTINSVGYRHSGVYTCTSQNEAG-VATQSAELKVNEPPQLMPLTFGD--DVLNEGEFAQLFCIVRVGDEPLTLTWALQGDVI-----------SSEPGLSTTQLAVGYXHSG-TYTCTVTNKA-----GSDSQSAILKVNEPPRILPLTFGQEVMNEGQFAQVVCIVTEGDEPLSIGWXLQGXXIXSXPSLSXSSLG-----TRTSMLTIQSIGYRHSGVYTCKATNK--AGSGSQXAVLRVNEPPEILPL--SFGKP-----------SMDEGEFAQIVCIVSRGDEPLSITWSLYGAVISSEPSISTN-----LLGSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQSASLIVNESPSILPFSFGAESVKEGDINQLTCLVNRGDEPLT---ITWSLKGDVVSSXPSITTTMIGTRASMLMITNVGYRHSGTYTCRATXSAGXA--THSAELKVNESPSILPFSFGSNNVKEGDITQLTCLVSRGDEPVRITWSLKGDVISSEPS-------------ITTTMFGTRT--SLLMITNVGYRHSGXYTCRASNAAGXATHSAELNVN---ESPSIL-AFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKG 1162          

HSP 4 Score: 75.0998 bits (183), Expect = 9.542e-14
Identity = 164/742 (22.10%), Postives = 271/742 (36.52%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVW-YRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-GPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNK-IISCYAINNDLGETVVQTHTISVLYPP-------GKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLG-------IDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDIT-DAVMVVTGETAHFECVVDA--KPITADTISWSRVGFDMEGRTSMQN---GTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNY 719
            G+   L C+   G+    + W  +GD            E     T +A    ++  Y C   N    +  +A  +L V   P  + +  G    N G      CI T  + P  + W +         ++   S      TR+++  +     R+  + +C A N     +  Q   + V  PP       GKPS         MDEG+  ++ CI   G+   ++TW     +I  +++    T       S L + +  Y  NG +Y C+A N     E+G +  + +L V   P  +      E  K G    L C     +    + W          +G++ V S P   ++TT ++G       I      H  T+ C AT    G + +       +  S L     F    + + D T     V  G +          P  +T+        + P+IT          +  +LL IT      +G Y  + +N+ G +    +++V    +I   +  +  V  G+     C V    +P+T   I+WS  G  +    S+     GT    L + NV    +G + C A N  +    T S  L V + P I E    S + ++     +    ++G   +  +WS  G V+S EP              I   +I   T  S L I NV  R  GSY C A N  G + ++  L V      P ++N F++
Sbjct:  668 GEFAQLFCIVRVGDEPLTLTWALQGD--------VISSEPGLSTTQLAVGYXHSGTYTCTVTNKAGSDSQSA--ILKVNEPPRILPLTFGQEVMNEGQFAQVVCIVTEGDEPLSIGWXLQGXXIXSXPSLSXSSLG----TRTSMLTIQSIGYRHSGVYTCKATNK--AGSGSQXAVLRVNEPPEILPLSFGKPS---------MDEGEFAQIVCIVSRGDEPLSITWSLYGAVI--SSEPSISTNLLGSRXSMLXIQSVGYRHNG-IYTCQAKN-----EAGSMRQSASLIVNESPSILPFSFGAESVKEGDINQLTCLVNRGDEPLTITWSL--------KGDV-VSSXP---SITTTMIGTRASMLMITNVGYRHSGTYTCRATXSA-GXATHSAELKVNESPSILP--FSFGSNNVKEGDITQLTCLVSRGDE----------PVRITWSLKGDVISSEPSITTT-----MFGTRTSLLMITNVGYRHSGXYTCRASNAAGXATHSAELNVNESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLT---ITWSLKGDVVSSEPSITTTMIGTRVSMLMITNVGYRHSGTYTCRATN--SGGTATHSAELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEP-------------SITTTMIGTRT--SMLMITNVGYRHSGSYTCRASNAAGSATHSASLLVKE----PPIINPFSF 1322          

HSP 5 Score: 72.4034 bits (176), Expect = 6.527e-13
Identity = 170/740 (22.97%), Postives = 276/740 (37.30%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVW-YRGDEQIDFSYYTTG----RESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-GPTEANVGDTLSFDCITTNSNPPAIVQWVV--DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGT-IMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLT-ANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWR-RNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV-TGETAHFECVVDA--KPITADTISWSRVGFDMEGRTSMQN---GTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCR-AQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYN 722
            G    L C+ + G+    + W  +GD        TT     R S  M T V         Y C+A  T S    T    L V  +P  +    G      GD     C+ +  + P  + W +  D  S++ + T         + TR+++ ++     R+        +N  G     TH+ + L     PSI     G+  + EG   +LTC    G+   T+TW  +GD +   +++    T       S L +T   Y  +G  Y C A+N       G  + +  L V   PI             G  A L C     +    + W  + D +S     E  + +   G    T++L I      H  ++ C A+N     S     ++ VK    +NP   F  +P+ + D       V  G +          P  +T+        + P++T          +  ++L IT+     +G Y  + +N  G      ++ +     I+  + +  V+  G  A   C V    +P+T   I+WS  G  +    S+     GT +  L +  V    +G+F C A+N    ++   ST   + + PRI      S++  D+   A LTC  + G   V  SWS  G VIS EP              I   ++   T  S L I +V  R  G Y C A+N  G+  Y   L V   P+    +  F+++ N
Sbjct:  946 GDINQLTCLVNRGDEPLTITWSLKGDVVSSXPSITTTMIGTRASMLMITNVGYRHSGT--YTCRA--TXSAGXATHSAELKVNESPSILPFSFGSNNVKEGDITQLTCLVSRGDEPVRITWSLKGDVISSEPSITTTM------FGTRTSLLMITNVGYRHSGXYTCRASNAAGXA---THS-AELNVNESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKGDVV---SSEPSITTTMIGTRVSMLMITNVGYRHSG-TYTCRATN-----SGGTATHSAELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVS----SEPSITTTMIGTR--TSMLMITNVGYRHSGSYTCRASNA--AGSATHSASLLVKEPPIINPF-SFGAEPVNEGDVAQLTCLVRRGDE----------PLSITWSLKGDIISSEPSMTTT-----MFGTKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKINEPPQIAPFSFSPHVIDEGSYAQISCSVTKGDEPLT---ITWSLHGAVISSEPSITTTMIGTRTSILIISEVGYRHSGKFTCRASNPAGSVR--YSTEXSINEPPRIVPFSFGSESI-DEGSFAQLTCSISHGDVPVSISWSLKGDVISSEP-------------SITTTMVGGRT--SILIISSVGYRHSGDYTCNAKNIAGMVTYTSTLLVNEAPE----IGPFSFSKN 1613          

HSP 6 Score: 63.1586 bits (152), Expect = 4.198e-10
Identity = 144/714 (20.17%), Postives = 268/714 (37.54%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQF-APDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESER-GGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWR-RNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVT-GETAHFECVVDA--KPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPD 709
            G++V+  C ++ GNP+ EV W +  + I+         + N     +  +D+   Y+C  +N       TA++ L  +F  P+ ++    T    G  LS  CI    +P   ++W +  +  +    +   S R    +  S++++            C A ++ +G+ +     ++V    G P +  + P  ++  G+S  +TC  +AG P++ + W +      G     ++ +T     + +       ++  VY C A N     E     S + +SV  +P  +      +    G  A + C     +    + W  + D +S E  G    + GP       ++L I      H  T+ C A N+     ++   ++TV     L PL        F  D    I    E + L+  +S    P  +++        + P ++       +I +  ++L I       +G Y     N  G +    ++ V     +  +T    V+  GE A   C+V    +P+   T++W+  G D+    S + G ++  L    V    +G + CT  N      D++S  L V + PRI       +  ++ +   V+    +G   +   W   G  I   P    S                  T  S L I+++  R  G Y C A N+ G       L V   P+
Sbjct:  200 GRSVTFKC-NYKGNPIKEVFWLKNGKSIN--------HNENTLRINSVKKDDRGMYQCFIRNEQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISCI-AKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQA-SSKVGK-ISHMAKLNVF---GAPYVKKMRPMKVV-AGKSMFITC-PVAGYPISNIVWEK-----DGRQLPFNDRQTVFPNGTLIISDIQRKEDASVYTCVAXN----DEGYSARSDLEVSVMERPEIVPFFFGSDSVDEGLYAQVSCIVQRGDTPIKISWSLKGDTVSSE-PGMTTSQVGP-----RASILSIHSVGYRHSGTYTCTAQNK--AGIISYSTSLTVNEPPSLMPL-------TFGRD----ILDEGEFAQLICIVSKGDEPLTMSWTLKGDDVSSEPGLSTT-----KIGTRTSILTINSVGYRHSGVYTCTSQNEAGVATQSAELKVNEPPQLMPLTFGDDVLNEGEFAQLFCIVRVGDEPL---TLTWALQG-DV---ISSEPGLSTTQLA---VGYXHSGTYTCTVTNKAGS--DSQSAILKVNEPPRILPLTFGQEVMNEGQFAQVVCIVTEGDEPLSIGWXLQGXXIXSXPSLSXSS---------------LGTRTSMLTIQSIGYRHSGVYTCKATNKAGSGSQXAVLRVNEPPE 836          

HSP 7 Score: 61.2326 bits (147), Expect = 1.590e-9
Identity = 182/898 (20.27%), Postives = 341/898 (37.97%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQI--DFSYYTTGRESRNMYTFVASSEDNNK-RYRCKAKNTVSVEPLTADIVLSVQFAPDKVT-------IEGPTEA---------NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPN-DRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITG--IDPGTIMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE-IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF---DVTYRWANPY---NENIPNIT----LDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDA-KPITADTISWS----RVGFDMEGRTSMQ-----NGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELH------------IKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTL----LNVFNYTYNSVYLTWIPGFD---GGHKQSFIIRYRKKGS---KTYQYVDV----HHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMV--KPEDKDVERTISQGIILT 826
            G    + CV + G+    + W    E I  +    T     R     + S++ +++  Y C+A+N   ++  +A++ ++  F   +V         E P  A         N GD     C+ T+ + P ++ W     +   N  + T +       R ++  +   N       +C A N    +  ++THT  +     +P + G    P  +M+EG   +LTC+  +G+   ++TW + GD++    TQ    T       S L + +  Y   G  Y C ASN A     G  S T  L V   P  + +     V   G  A + C     +    + W  + G S+E+ G+ I +     G    T++L I+     H   + C A+N+    S    + + V              +P + ++PTD  F+  +GS+    I  +++ F    + +R A      NE  P  +    LD  N   +  I   L I   +K + G Y  K +N  G      KI V    +          +  +    +C     KPI    + W+    R+   ++ R +++     +G  S  +++    + D+  F C A N      D  S  L++++ P   E+P + K      +T            V  SW        Q P    S      +Y I++    + +W++++             + ++   +   +  +AQN  G+S  +  + + +  + P+     + V      ++ ++W P       G    + + Y+   +   K Y +  V         +  ++ +L++ T+Y+  V+AFN +G    + E +        EG     P+D D+    SQ I +T
Sbjct: 1714 GDFAQVTCVVNRGDLPLTITWSFHGENIGPNTGISTNNLGPRISLLVINSAQHHHQGEYICQAQNKAGIQTHSAELKVNGTFILMRVADYFYFWFSEPPLIAPFSFANEVMNEGDFAQISCVITSGDLPLLITWSFHGNTIGTNTGIATTN----LGPRMSVLAINSVNYSHQGKYTCQAKN----KAGIRTHTAELKV--NEPPMIGPFAFPKEVMNEGNFAQLTCVVTSGDLPLSVTWTFHGDKV---GTQTGITTTNLGPRLSLLVINSVGYRHRGN-YTCXASNNA-----GHDSYTAKLRVNEPPSLVPLNFGKNVFSEGSFAQVVCTITEGDEPIKISWSFH-GESLEDSGQNIGIAITNIGSR--TSILIINSVEHKHRGNYTCSASNKAGNSSDTAQLKINV--------------RPRWTVEPTDKHFA--QGSE--AKIDCKSDGFPQPKIVWRKAIGLPVSNEQXPTPSDYKDLDVSNDRNMKIIDGTLIIKNIQKSNEGFYLCKASNGIGGISAVAKISVQAPPSFEIKKRTQTALISDNTVLQCEAKGEKPI---GVLWNMNNKRLDAKVDDRYTIREEVIDDGVLST-ISIKRTERGDSALFTCVATNAFGS--DDTSINLIIQENP---ETPYDLKVLDKAGRT------------VKLSW--------QTPYNGNSNLT---RYVIEFKPT-KGSWKNDIDHVLVPGDQNQAVVYSLKPANSYHFRIVAQNVIGVSGPSDTITIETAEEAPSGPPVDIRVSAVDQQTLRVSWKPPLTEHWNGDILGYYVGYKATANSEEKPYLFETVEFRKEQGHEHQLQISNLEVFTEYAVVVQAFNKIGQGPMSXEXLX----HXAEGAPTESPQDIDLVTLSSQSIKVT 2534          

HSP 8 Score: 53.9138 bits (128), Expect = 2.832e-7
Identity = 73/315 (23.17%), Postives = 118/315 (37.46%), Query Frame = 0
Query:  402 PIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWAN--PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVT-GETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRP-----RIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            P F      T    +EG    +     A+P   ++RW      N N   + LD     R+  +   L I EA+ ED+G+Y   VNNS G   ++  + V    ++ D+     VV  G +  F+C     PI    + W + G       S+ +  N++ +   +V K+D G + C   N     + T    L  R  P       EE+               ++C A+G P     W   G  +  E          D   ++           S L+I  V ++  G+Y+C A ++ G   +   LNV   P
Sbjct:   89 PKFSTVGKSTTLEFKEGLSFALICPAMASPIP-SFRWYKFMEANNNKKLVKLDD----RVKXVGGTLIIKEAKVEDSGKYLCMVNNSVGGESVETVLTVTAPLSV-DVEPKTQVVDYGRSVTFKCNYKGNPI--KEVFWLKNG------KSINHNENTLRIN--SVKKDDRGMYQCFIRNEQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLS------ISCIAKGDPSPNIKWFIYGKEVKNE-GAITVGSFRDANGDVT----------SHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFGAP 370          

HSP 9 Score: 53.1434 bits (126), Expect = 5.557e-7
Identity = 114/534 (21.35%), Postives = 192/534 (35.96%), Query Frame = 0
Query:  195 IMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRR-------NDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEE-----LGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSV-EEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVT-GETAHFECVVDAKPITADTISWS----RVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            IM+EG   +LTC+   G+   T+TW + G+ I    T     T       S L + +    +   Y C+A N A     G  + +    V   P+        EV   G  A + C     +    + W         N G+S  N G  R+           ++L I+     H   +IC+A N+        E   +G  + ++   +      F + P+  I P      V  EG    ++  + +    +   W+   N    N  + + N+ PR+    ++L I        G+Y  +  N  G      ++ V     I        V+  G  A   CVV +  +   +++W+    +VG    G T+   G     L + +V     G + C A+N      D+ +  L V + P +          S+     V+    +G   +  SWS  G            E +ED    I   + +  +  S L I +V  +  G+Y C A N+ G S     L +  RP
Sbjct: 1614 IMNEGDFAQLTCVVNRGDLPLTITWSFHGENI---GTNTGIITSNLGPRMSMLVINSAQHHHQGEYTCQAINKA-----GIRTHSAEXKVNEPPLIAPFSFANEVLNEGDFAQVTCVVNRGDLPLTITWSFHGENIGPNTGISTNNLGP-RI-----------SLLVINSAQHHHQGEYICQAQNKAGIQTHSAELKVNGTFILMRVADYF--YFWFSEPPL--IAPFSFANEVMNEGDFAQISCVITSGDLPLLITWSFHGNTIGTNTGIATTNLGPRM----SVLAINSVNYSHQGKYTCQAKNKAGIRTHTAELKVNEPPMIGPFAFPKEVMNEGNFAQLTCVVTSGDLPL-SVTWTFHGDKVG-TQTGITTTNLGPRLSLLVINSVGYRHRGNYTCXASNNAG--HDSYTAKLRVNEPPSLVPLNFGKNVFSEGSFAQVVCTITEGDEPIKISWSFHG------------ESLEDSGQNIGIAITNIGSRTSILIINSVEHKHRGNYTCSASNKAGNSSDTAQLKINVRP 2103          
BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Match: EMLSAP00000002034 (pep:novel supercontig:LSalAtl2s:LSalAtl2s136:1321748:1354066:-1 gene:EMLSAG00000002034 transcript:EMLSAT00000002034 description:"maker-LSalAtl2s136-augustus-gene-13.18")

HSP 1 Score: 79.7221 bits (195), Expect = 3.712e-15
Identity = 105/433 (24.25%), Postives = 173/433 (39.95%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQI-----DFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKN-TVSVEPLTADIVLSVQFAPDKVTIEGPTEANV-GDTLSFDCITTNSNPPAIVQWVVDSRSAQEN-----------FTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQ-----THTISVLYPPGKPSITGIDPGTI-MDEGQSRRLTCISMAGNP--LATLTWYRGDEIIQ--GTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKA---GKKAYLFCETGSSNPT--ANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDK 401
            GQ + L+C S GG P+ +V WY G + +      F         +N      S  D    + CK++N  +   P+++ + + V   P    + G  E  +   T++  C T  + P A++ W   SR   E              V  + E   +ET+S IS      + N+ I+C A NND+ E+  +     + T+SVLY P       I P  I ++E  +  + C     NP  L  + WYR  +  +    T  D E    + A   L L     D    Y C   N   + ES   S    L V ++ + ++++ DP  P +    +   LFC+    NP   + V W   DG  ++   +    +     ++  + L ++  +R     F C+  NE      +D   + V    F +P   FI K
Sbjct:  164 GQELRLLCESGGGKPIPKVTWYNGSDILPGKSSSFEEPDGTGTGKNEVRIKLSRGDLGSHFTCKSENEAIRDNPMSSSVQVDVNLRPLNTEVIGSDEPILESSTVTLMCRTIGARPAAVITWYNGSRPFAEQSPGVKALQKPPLEVTVKQEDNTYETQSRISFTASRFENNEFITCEA-NNDILESFQEEPQRDSITLSVLYTPK----VEIYPTNITVNESSTVTIYC-KFDANPKNLTNVQWYRNGQFYEEGNLTAVDIEDGLSHVA---LILKNVSRDASGTYNCLLENEIGSGES---SEPSILDVYYE-VEVSLRMDPRSPISEADERNVTLFCDVKMGNPNVLSGVKWFM-DGELLKELPQCDDENNMDLCDIDPSKLLLEHVSRHFSGNFSCQGVNEAGWSDFSDEKILEV----FYSPGTAFIQK 578          

HSP 2 Score: 73.1738 bits (178), Expect = 3.860e-13
Identity = 121/523 (23.14%), Postives = 199/523 (38.05%), Query Frame = 0
Query:  170 VVQTHTISVLYPPGKPSITGIDPGTIMD-------EGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGID-----------VEARDHGVTFI-CEATNEEL----GESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVT----YRWANPYNE-NIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS--EGKSRLKIKIDVLYGATISDITDAVMVVT-GETAHFECVVDAK---PITADTISWSRVGFDMEGRTSMQNGTNSM--------HLTVYNVSKEDAGEFLCTANN--GIADMKDTKSTFLLVRQRPRIEESPANSKAASD-----KRKTAVLTCRAQG---TPDVVFSWSRMGSVIS 640
            VVQ   ++ L  P   SI+  +   I +       EGQ  RL C S  G P+  +TWY G +I+ G +   +E +     ++E+ +  +  D G  + C++ N A      P+SS++ + V  +P+   V    E         L C T  + P A + W        E    ++    P    L   V   D             +R     FI CEA N+ L     E   D IT++V            +  P  +I PT+   +V E S + +     ANP ++T    YR    Y E N+  + ++ D +  +  I  L N++   ++ +G Y   + N    G+S     +DV Y   +S   D    ++  +  +     D K   P     + W   G ++       +  N+M         L + +VS+  +G F C   N  G +D  D K   +          SP  +    D     K ++  LTC        P   + W R G V++
Sbjct:  128 VVQLVKLTTLAEPKFVSISLPEDKNISNSIIGPYTEGQELRLLCESGGGKPIPKVTWYNGSDILPGKSSSFEEPDGTGTGKNEVRIKLSRGDLGSHFTCKSENEAI--RDNPMSSSVQVDVNLRPLNTEVIGSDEPILESSTVTLMCRTIGARPAAVITWYNGSRPFAEQSPGVKALQKP---PLEVTVKQEDNTYETQSRISFTASRFENNEFITCEANNDILESFQEEPQRDSITLSV------------LYTPKVEIYPTN--ITVNESSTVTIYCKFDANPKNLTNVQWYRNGQFYEEGNLTAVDIE-DGLSHVALI--LKNVS---RDASGTYNCLLENEIGSGESSEPSILDVYYEVEVSLRMDPRSPISEADERNVTLFCDVKMGNPNVLSGVKWFMDG-ELLKELPQCDDENNMDLCDIDPSKLLLEHVSRHFSGNFSCQGVNEAGWSDFSDEKILEVFY--------SPGTAFIQKDHDEVVKGESLSLTCGVSDPGRPPASTYIWKRGGHVVN 616          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|20177993|sp|O60500.1|NPHN_HUMAN (RecName: Full=Nephrin; AltName: Full=Renal glomerulus-specific cell adhesion receptor; Flags: Precursor)

HSP 1 Score: 318.931 bits (816), Expect = 6.737e-90
Identity = 238/837 (28.43%), Postives = 381/837 (45.52%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYT--TGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDL-GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGT--TQDDDETETKNYAQS------ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRT-----SMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSE--NVAVETLSYLEGMVKPEDK 813
            GQ++ L CV+ GGNPLA + W +  + +  ++ T  T   +R++       ED+  +  C+A N+VS       I L V F P  + I G         ++  C++ +S P  +++W +  R          +   GG  + SN++ L    D    ++C A +     ET  ++  ++V YP  K  I G   G  +  G   RL C+++ GNP  +L WY+    +  +   Q+       +  +S      EL L    SDN   + C+A         G LS++  L+V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E       R  PF G+     + + V +RDHG    C A + EL E+V+          SF    ++ + +P F  +    + +VE+G  L+  +SV ANP    + W            L     PR  I S    +L N+T A   D G Y+    NSEG +  ++++DV Y  TI  + D   V  G +    C VDA PI     +W R+G D E ++      +  G     L +++     AG + C  +NG+A     +   L+VR  P++E     +K  AA D   +A L CRA+G P++VF+W++ G  +          +++D +Y  ++         S L I NVS+ +DY  + C A N  G  +  + L   SRPD P+ L V + T +SV L W PGFDGG  Q F IRY   G+  + YVDV      +F +  L+  T+Y   + A N LGDS    +   + + T    +   +PED+
Sbjct:  258 GQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKA---------GQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDK-EGERLEGVAA-PPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVS----------SFYR--LNVLYRPEFLGEQVLVVTAVEQGEALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSSGALHLWNVTRA---DDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGEDEEDQSLDDMEKISRGPTG-RLRIHHAKLAQAGAYQCIVDNGVAP-PARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPL----------DLQDPRY-TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQ 1048          

HSP 2 Score: 108.612 bits (270), Expect = 1.626e-22
Identity = 160/707 (22.63%), Postives = 289/707 (40.88%), Query Frame = 0
Query:   53 NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV-----GDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATA--QESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-------VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGAT----------ISDITDAVMVVTGETAHFECV-VDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGEFL-CTANNGIADMKDTKSTF--LLVRQRPR-IEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP-----DTPTLLNV 716
            ++  Y C+  ++ +  E ++  ++LS+   P K+ +  P    +     G     +C++ ++ P   +  ++  ++  + +  V   S++  +   +   V    +D  +++ C A +  L   +  + T++VL+PPG P I   G+D G +   GQS  L C++  GNPLATL W +  + +      +    T+  A+S L +T    D+G    CEA N  +A  QE G     ITL V F P  I +       +  K   L C + SS P   V+ R   G       E  V  G  GG+++ + L       D+G+T  CEA +E   +            ++F   LI  +  P   +     I    EG  L       +V +++  NP + +  W   Y +   + T+    +P+     +L ++ ++    +         S+ +++   K   L  +T          ++ + +A  +  G+  +  CV V + P     +SW + G  +EG     R +   G+ +    +  VS  D G+ + C A++  A++++T S+F  L V  RP  + E      A         ++  A   P+  F+W+  G  +S                        R+     LH+ NV+  D G Y    QN EG +   + L+V   P       PT +NV
Sbjct:  105 DDAEYECQVGRSEMGPELVSPRVILSI-LVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVR-AGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTE---HTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHG-----ITLQVTFPPSAIIILGSASQTE-NKNVTLSCVSKSSRP--RVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTK------------ETFKKSLILNVKYPAQKL----WIEGPPEGQKLRAGTRVRLVCLAIGGNP-EPSLMW---YKD---SRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPV--NLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHS--AELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPE-AFNWTFRGYRLSP-----------------AGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNV 753          

HSP 3 Score: 106.686 bits (265), Expect = 5.630e-22
Identity = 160/729 (21.95%), Postives = 279/729 (38.27%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEV-VWYRGDEQIDFSYYTTGRESRNMYTFVAS------SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP----TEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRG-DEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPK--AGKKAYLFCETGSSNPTANVIWRRNDGLSIEN---QGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAG-RYKFKVNNSEGKSRLK--IKIDVLYGATISDITDAVMVVT----GETAHFECVVDAKPITADTISWSRVGFDME---GRTSMQNGTNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-----LTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQN 691
            GQ   + CVS    P  ++ +   G    D S        + ++T  A+      S DN +   C+A +     P+ A   ++V F P    IE P         G +L   C+    NP A +QW+ + +     +     +E      RS + + + P D    +SC A N+    T     T+ V +PP    I G    T   E ++  L+C+S +  P   L W+ G  +++       D     + + S L   A   DNG+   CEA + A  +E+     ++ L+V++    + ++  PE  K  AG +  L C     NP  +++W ++     E+   Q   RV  G     G+  +  L +     D+   F C+A   +L  S    +                   P  ++       ++  G  L +  +SV +NP  V   W +   E +  +       P   S      + +    D G R   + +++E +  +    +++VLY      + + V+VVT    GE A     V A P   +  +W+  G+ +    G       + ++HL  +NV++ D G  +  C  + G A+           R R  +  +P   +A  D  +  V     + C     P +  +F+W R+G        EDE ++  D+  +I      R      L I +      G+Y CI  N
Sbjct:  153 GQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWG----TEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQT---ENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKET--FKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAG--QLSASTQLAVQF-----------------PPTNVTILANASALRPGDALNLTCVSVSSNP-PVNLSW-DKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFYRLNVLYRPEF--LGEQVLVVTAVEQGE-ALLPVSVSANP-APEAFNWTFRGYRLSPAGGPRHRILSSGALHL--WNVTRADDGLYQLHCQNSEGTAE----------ARLRLDVHYAP-TIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLG--------EDEEDQSLDDMEKISRGPTGR------LRIHHAKLAQAGAYQCIVDN 820          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|325511343|sp|Q9QZS7.2|NPHN_MOUSE (RecName: Full=Nephrin; AltName: Full=Renal glomerulus-specific cell adhesion receptor; Flags: Precursor)

HSP 1 Score: 313.153 bits (801), Expect = 7.814e-88
Identity = 244/845 (28.88%), Postives = 383/845 (45.33%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDD---------ETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE------IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFD-----MEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK-PEDKD 814
            G+ + L C++ GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS E     I L V F P  VTI G T  +    ++  C+T +S P  +++W +  R          +   GG  + SN+++L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +LTW +    +    Q  +         E     +++ EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N        LS++ +GE       + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF    ++ +  P F  +    +  VE+G  L+  +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G D     ++    M  G+    L +       AG + C  +NG+A         L+VR  P+++     +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E KY+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    S  + Y+DV  +   +F +  LK  T+Y   + A N LGDS  T + + V   +   G+ + PED D
Sbjct:  272 GENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSR-ELVLIIGPPDNLAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVN--------LSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVS----------SFYR--LNVLYPPEFLGEQVRAVTVVEQGQALL-PVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEDEEELNLDDMEKMSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHKYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITT--PGLDQAPEDTD 1060          

HSP 2 Score: 109.768 bits (273), Expect = 6.319e-23
Identity = 90/387 (23.26%), Postives = 165/387 (42.64%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID--FSYYTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVP--KAGKKAYLFCETGSSNPTANVIWRR-----NDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEA 369
            GQ   + CVS    P  ++++ +G   ++   S    G E +  +T         S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   CI    NPPA +QW+ + +      ++   +E       S + + + P D    +SC + N+   ET  ++ T+ V +PP   +I G    T   E ++  L C++ +  P   L W+ G   +  T +   D     + + S L L     DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++ W +     ND    +    +++ S    G+  +  L + +   D+   F C+A
Sbjct:  167 GQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCIARGGNPPATLQWLKNGKPV----SIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILG---STSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKA 544          

HSP 3 Score: 63.5438 bits (153), Expect = 8.307e-9
Identity = 60/236 (25.42%), Postives = 101/236 (42.80%), Query Frame = 0
Query:  159 CYAINNDLGETVVQTHTI-SVLYPPGKPSITGIDPGTI-MDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDE--TETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKP----ITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            C    ++LG  +V    I S+L  P    +T     T+    GQ   +TC+S    P   + + +G   ++  +   +E   E   + ++E  +T   SDNG +  CE SNPA A    P+ ++ T+++ F P    I     ++  V +AG+   L C     NP A + W +N        G    ++      +  +VL + V   DHG    C++ N    E+    IT+ V
Sbjct:  125 CQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILFPPGPPVIDWPGLNEGHV-RAGENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQA------VAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQV 350          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|325511344|sp|Q9R044.2|NPHN_RAT (RecName: Full=Nephrin; AltName: Full=Renal glomerulus-specific cell adhesion receptor; Flags: Precursor)

HSP 1 Score: 302.368 bits (773), Expect = 3.572e-84
Identity = 228/821 (27.77%), Postives = 371/821 (45.19%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES--RNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETK--------NYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR--IYS--IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSK--AASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKY-EIKYNVIDRMTWESELHIKNVSS-RDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAV 798
            G+ + L C + GGNP A + W +  + +  ++ T   ++   ++       ED+  R  C++ N+VS       I L V F P  +TI G    +    ++  C+T +S P  +++W +  R          +   GG  + SN++ L+   D    ++C A ++    ET  ++ T++V YP  K  I G   G  +  G   RL C+++ GNP  +L W++    +    Q  +    +        +    EL L     DN   + C+A         G LS++  L V+F P  + + ++    + G    L C + SSNP  N+ W + +G  +E+    + +S PF G+  +  + + V +RDHG    C A +E L E+V+          SF     + +  P F  +    +  VE+G  +++ +SV ANP    + W            L     PR  I S     L N+T A   D G Y+    NSEG +   +K+DV Y  TI  + D   V  G +    C VDA PI  +  SW R+G      +++    +  G+    L +       AG + C  +NG+A         L+VR  P++++    +K  AA D   +A L CRA+G P++ F+W++ G  +          +++D +Y E +Y+    +   S L I NVS+ +DY  + C A N  G     + L   SRPD P  L V + + +SV L W PGFDGG  Q F IRY    +  + +VDV  +   +F +  LK  T+Y   + A N LGDS  T + + V
Sbjct:  272 GENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKA---------GQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDK-EGERLEDVAA-KPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVS----------SFYR--FNVLYPPEFLGEQVRAVTVVEQG-QVLLPVSVSANPAPEAFNW------TFRGYRLSPAGGPRHRILSGGALQLWNVTRA---DDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTG-RLRIRQAKLSQAGAYQCIVDNGVAPAA-RGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPL----------DLQDPRYTEHRYH--QGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV 1045          

HSP 2 Score: 97.4413 bits (241), Expect = 3.803e-19
Identity = 164/754 (21.75%), Postives = 283/754 (37.53%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYR-GDEQIDFSY-YTTGRESRNMYT-----FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGP--TEANV--GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD-DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIE---NQGEIRVRSGPF--GGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD-TIF----SVEEGSDL-VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK---IKIDVLYGAT-ISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG-----TNSMHLTVYNVSKEDAG--EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDV--VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRP--DTPTLL 714
            GQ   + CVS    P  ++ + + G   +D S     G E +   T      +  S DN +   C+  N     P+ A   +++ F P    I+ P   E +V  G+ L   C     NPPA +QW+ + +     +     +E       S + + + P D    +SC + N+    T  ++ T+ V +PP   +I G        E ++  L C++ +  P   L W+ G   +  T +   D     + + S L       DNG+   CEA + A ++E+     ++TL+V++    + ++  PE    + G +  L C     NP  ++IW ++     E    Q   RV+ G     G+  +  L + +   D+   F C+A   +L  S                       + +    PT+ TI     ++  G  L +  +S+ +NP  V   W +   E + ++     + P   S  +          D G+      +SE          + +VLY    + +   AV VV          V A P   +  +W+  G+    R S   G      +   L ++NV++ D G  +  C  + G A+             R   + +  N   + D      + C     P +  +FSW R+G       EE+E   ++D +   K +          L I+       G+Y CI  N     +R  V L V   P  D PT L
Sbjct:  167 GQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWG----TEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILG---SVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKET--FKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAG--QLSAST----------------------QLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNP-PVNLSW-DKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANP-APEAFNWTFRGY----RLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVD------IVCTVDANPILPEMFSWERLG-------EEEEDLNLDDMEKVSKGST-------GRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPL 860          

HSP 3 Score: 86.2705 bits (212), Expect = 1.150e-15
Identity = 86/367 (23.43%), Postives = 159/367 (43.32%), Query Frame = 0
Query:   33 YYTTGRESRNMYTFVASSED--NNKRYRCK-AKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN-----VGDTLSFDCITTNSNPPAIVQWVVDSRSAQE-NFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSI--TGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELG-ESVNDGITMTVK 387
            Y   G  ++  +  +  + D  ++  Y C+  ++ +  E ++  ++LS+  +P KV +  P   +      G      C++ ++ P   + ++   R+  + +  V   SE     T +   V+   +D  +++ C   N  L   +  + T+++L+PPG P I   G++ G +   G++  L C +  GNP ATL W +  + +      +     +  A S L +T    D+G    C++ N  +A   G    +ITL V F P  I +       +  K   L C T SS P   + W    G  +    E  V  G  GG+++ + L   V   D+G+   CEA ++    E+    +T+ VK
Sbjct:   97 YSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSP-KVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVR-AGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTE---HAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSA---GTQERSITLQVTFPPSAITILGSVSQSE-NKNVTLCCLTKSSRPRVLLRWWLG-GRQLLPTDET-VMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVK 452          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|75025812|sp|Q9U3P2.1|SYG2_CAEEL (RecName: Full=Synaptogenesis protein syg-2; AltName: Full=Synaptogenesis abnormal protein 2; Flags: Precursor)

HSP 1 Score: 279.256 bits (713), Expect = 2.229e-76
Identity = 251/962 (26.09%), Postives = 401/962 (41.68%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDF--SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSA----QENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDE--IIQGTTQDDDETETKNYAQSELHLTA-------NYSDNGMVY----------------------------------------RCEASNPATAQESGPLSSTITLSVRFKPITIAVK---SDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTT--------NVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWAN---PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDY-GSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLS---YLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYV---KRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMS 887
            G  V++ C   GGNP  +V WY  ++++    +  T  +E +N+Y+F+AS  DN   Y C+A N+ +  P    + L V + P  V + G +    G + +  C +  SNP + + W+++ RS     Q  F V          +  +  + +  +  N  + C A N + G +  Q H I ++ PP  P ITG++      EG    +TC +  GNPLA L+WYRG E  +++G  +       K    S L L          +S N   +                                        +CEA+N A  +   PL  +  LSV + P  + ++   S  +    GK A L C T SSNP A++ W+ +         + +V  G    N TT        NVL         G    C A + E   SVN    + V     + P    ++      DP   + S  EG     N++VR NP    ++W     P++  I           R+++   +L+  +    DAG Y     NS G + + IK+ V Y A I+ I+  V+  TG+T   EC  D +P  A+ I W + G   E   +   G     L + N +++ +GE+ C A+NGI  +     TFLLV   PR+      +K A      A   C+    P V F W +   +I +             KY I    ID  T+ES L IKN+   DY     CIA+N  G    ++ +   + PD P  L + N T N++ + W P FDGG  Q F ++YR++       V+  H+   +  +  L     Y F +++ N  G +   +  V   TL+       M++ E   +   I   +I  +     L ++LNV+ I C+V    ++R +R   + +A      G  +   ++MY     N+ MS
Sbjct:  249 GDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESNIRYGSSANIQCKSLPSNPASQITWIINGRSVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQIN--VECMATNPE-GSSAKQ-HVIKIIAPPKAPIITGLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVKKKLVMSLLCLCEVVIMNFFRFSKNVFYFFRFTLFIECGVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDE---PLIESQYLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD-----DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNTSFPLNV-----MYPPKMLVN------DPVTVVMS--EGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIG----------RVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIHWYKNG---EILAAQHRGHKKAILRL-NATEQQSGEYTCKADNGIG-VPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEKDEQLIKK----------NSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHT---NLRLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNVAMIRNEKLKLTTWIPYILIFIV-----LFMLLNVV-ICCFVCNRTKKRKLR-EKTEMARTAINGGDVRQ--VQMYGTMMGNDGMS 1147          

HSP 2 Score: 80.1073 bits (196), Expect = 7.318e-14
Identity = 79/330 (23.94%), Postives = 141/330 (42.73%), Query Frame = 0
Query:   99 LSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRN---KIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG-TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSI--ENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFI-DKPIFDIDPTDTIFSVEEGSDL 421
            L+  C+  N  P   V W +D +    +  V+  S     +T +  + L+  +DRN   K+I+C A   +    +    T+ VL+PP  P++  +   + +  G +  + C    GNP   + WY  ++ +Q  +T D    E KN         A+ +DN   Y C A+N  T     P    + L V + P ++ +  +  + + G  A + C++  SNP + + W  N G S+    Q E  V +G    +   +V   ++    H +   C ATN E   +    I +    K+   P+I  + D+  F+ D  +     + G+ L
Sbjct:  148 LNITCVVPNVKPEPEVLWYMDGKVMSRD--VKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNI----YSFIASQNDNMAEYECRANNSRTGN---PKRKAMKLEVNYPPASVELFGESNI-RYGSSANIQCKSLPSNPASQITWIIN-GRSVPTPTQREFVVENG-IVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKA---PIITGLEDRKFFEGDIVNVTCEAQGGNPL 462          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|49036496|sp|Q9HCK4.2|ROBO2_HUMAN (RecName: Full=Roundabout homolog 2; Flags: Precursor)

HSP 1 Score: 125.561 bits (314), Expect = 1.107e-27
Identity = 173/682 (25.37%), Postives = 265/682 (38.86%), Query Frame = 0
Query:  195 IMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSEL------HLTANYSDNGMVYRCEASNPATAQESGPLSSTITL---SVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE-IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQN--LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG---FDMEGRTSMQNGTNSMH----LTVYNVSKEDAGEFLC----TANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSR-------------PDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQS-FIIR-YRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFN--DLGDSEFTSENVAVETLS-YLEGM----VKPEDKDVERTISQGIILTITLV 830
            I+ +G+   L C    G P  T+ WY+  E ++ T +DD  +         L      H   +  D G  Y C A N      S   S  + L     R  P  + V        AG+ A L C+    +P   + W++ D + I+++ E I +R    GG L  +    +    D G+ + C  TN  +GE  +D   +TV             ++P F   P + +   EE  +    +     P   T RW             D  ++PR  Y I++   L I +    D G Y     N  GK      + V             +V  G T  F C     P  A  + W + G        +    N   S+     LT+ N+ + DAG ++C     A + +A  +   +  L  R  P I + PAN   A D   TA+L C+A G P  V SW + G      P  D    I+++                 L IKN+   D G+Y C+A +  G + ++  L+VT               P  P+   V + T NSV L+W PG  G    S +II  + +  S ++Q V  +H  T  + V  L+  T Y F V+A N   L D    S+ V  + +S   +G+    V+ E  DV   +   ++LT T V
Sbjct:   41 IVSKGEPTTLNC-KAEGRPTPTIEWYKDGERVE-TDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGS-YVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVA-------AGEPAILECQPPRGHPEPTIYWKK-DKVRIDDKEERISIR----GGKLMIS----NTRKSDAGM-YTCVGTNM-VGERDSDPAELTV------------FERPTFLRRPINQVVLEEEAVEFRCQVQGDPQP---TVRWKK-----------DDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPA--VFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDG--TALLKCKATGDPLPVISWLKEGFTF---PGRDPRATIQEQ---------------GTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTV-ANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTV 652          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|1028066215|sp|D3ZB51.1|TUTLB_RAT (RecName: Full=Protein turtle homolog B; AltName: Full=Immunoglobulin superfamily member 9B; Flags: Precursor)

HSP 1 Score: 120.168 bits (300), Expect = 4.129e-26
Identity = 131/543 (24.13%), Postives = 230/543 (42.36%), Query Frame = 0
Query:  300 KAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRI-YSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDME-----GRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT-SRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVED-LKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSY-------LEGMVKPEDKDVERTISQGIILT 826
            K G    + C T   NP   V W + +G  +   G+ +V  G    +LT       V   D G  + C A + + GE+V+    +              +  P F + P + I +V    D ++     A P ++TY W   Y ++  N+   +D   R+   I   L I   + EDAG+Y    +NS G+S      + V Y A + ++   + V  G   +  C VDA+P  A  + W++ G  ++     G T M++G+    + +   ++E  G + C   N +  M  +    L+++  P     P         R+  ++ C A G P  V +W ++G                 +    K+N +        L  + +S  D+G ++C+A N       + +L V  + P  P  + V + +  +  ++W PG+DGG++Q+F +  ++     + ++ +      S+ + D L+  T Y FSV A N LG S F SE V V TL++       L  +  P      RT  QG++L+
Sbjct:  152 KEGGSITMTC-TAFGNPKPIVTWLK-EGTLLSASGKYQVSDG----SLTVT----SVSREDRGA-YTCRAYSIQ-GEAVHTTHLL--------------VQGPPFIVSPPENI-TVNISQDALLTCRAEAYPGNLTYTW---YWQDE-NVYFQNDLKLRVRILIDGTLIIFRVKPEDAGKYTCVPSNSLGRSPSASAYLTVQYPARVLNMPPVIYVPVGIHGYIRCPVDAEP-PATVVKWNKDGRPLQVEKNLGWTLMEDGS----IRIEEATEEALGTYTCVPYNTLGTMGQSAPARLVLKDPPYFTVLPGWEYRQEAGREL-LIPCAAAGDPFPVITWRKVG-----------------KPSRSKHNALP----SGSLQFRALSKEDHGEWECVATNVVTSITASTHLTVIGTSPHAPGSVRV-HVSMTTANVSWEPGYDGGYEQTFSVWMKRAQFGPHDWLSLSVPLGPSWLLVDSLEPETAYQFSVLAQNRLGTSAF-SEVVTVNTLAFPVTTPEPLVLVTPPRCLTANRT-QQGVLLS 633          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|49036483|sp|Q7TPD3.2|ROBO2_MOUSE (RecName: Full=Roundabout homolog 2; Flags: Precursor)

HSP 1 Score: 119.783 bits (299), Expect = 5.663e-26
Identity = 171/687 (24.89%), Postives = 273/687 (39.74%), Query Frame = 0
Query:  195 IMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSEL------HLTANYSDNGMVYRCEASNPATAQESGPLSSTITL---SVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE-IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPR-IYSIQN--LLNITEARKEDAGRY----KFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG---FDMEGRTSMQNGTNSMH----LTVYNVSKEDAGEFLC----TANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMG-SVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSR-------------PDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQS-FIIR-YRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFN--DLGDSEFTSENVAVETLS-YLEGM----VKPEDKDVERTISQGIILTITLV 830
            I+ +G+   L C    G P  T+ WY+  E ++ T +DD  +         L      H   +  D G  Y C A N      S   S  + L     R  P  + V        AG+ A L C+    +P   + W++ D + I+++ E I +R    GG L  +    +    D G+ + C  TN  +GE  +D   +TV             ++P F   P + +   EE  +    +     P   T RW             D  ++PR  Y I++   L I +A   D G Y    + +V   E  + L +++  +            +V  G T  F C     P  A  + W + G        +    N   S+     LT+ N+ + DAG ++C     A + +A  +   +  L  R  P I + P N   A D   TA+L C+A G P  V SW + G + + ++P       I+D+                 L IKN+   D G+Y C+A +  G + ++  L+VT               P  P+   V + + NSV L+W PG  G    S +II  + +  S ++Q V  +H  T  + V  L+  T Y F V+A N   L D    S+ V  + +S   +G+    V+ E  DV   +   ++LT T V
Sbjct:   41 IVSKGEPTTLNC-KAEGRPTPTIEWYKDGERVE-TDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGS-YVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVA-------AGEPAILECQPPRGHPEPTIYWKK-DKVRIDDKEERISIR----GGKLMIS----NTRKSDAGM-YTCVGTNM-VGERDSDPAELTV------------FERPTFLRRPINQVVLEEEAVEFRCQVQGDPQP---TVRWKK-----------DDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPA--VFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDG--TALLKCKATGEPLPVISWLKEGFTFLGRDPRA----TIQDQ---------------GTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTV-ANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTV 656          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|1028066211|sp|E9PZ19.1|TUTLB_MOUSE (RecName: Full=Protein turtle homolog B; AltName: Full=Immunoglobulin superfamily member 9B; Flags: Precursor)

HSP 1 Score: 119.013 bits (297), Expect = 1.026e-25
Identity = 109/445 (24.49%), Postives = 195/445 (43.82%), Query Frame = 0
Query:  398 FIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRI-YSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDME-----GRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT-SRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVED-LKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSY-------LEGMVKPEDKDVERTISQGIILT 826
             +  P F + P + I +V    D ++     A P ++TY W   Y ++  N+   +D   R+   I   L I   + EDAG+Y    +NS G+S      + V Y A + ++   + V  G   +  C VDA+P  A  + W++ G  ++     G T M++G+    + +   ++E  G + C   N +  M  +    L+++  P     P         R+  ++ C A G P  V +W ++G                 +    K+N +        L  + +S  D+G ++C+A N       + +L V  + P  P  + V + +  +  ++W PG+DGG++Q+F +  ++     + ++ +      S+ + D L+  T Y FSV A N LG S F SE V V TL++       L  +  P      RT  QG++L+
Sbjct:  224 LVQGPPFIVSPPENI-TVNISQDALLTCRAEAYPGNLTYTW---YWQDE-NVYFQNDLKLRVRILIDGTLIIFRVKPEDAGKYTCVPSNSLGRSPSASAYLTVQYPARVLNMPPVIYVPVGIHGYIRCPVDAEP-PATVVKWNKDGRPLQVEKNLGWTLMEDGS----IRIEEATEEALGTYTCVPYNTLGTMGQSAPARLVLKDPPYFTVLPGWEYRQEAGREL-LIPCAAAGDPFPVITWRKVG-----------------KPSRSKHNALP----SGSLQFRALSKEDHGEWECVATNVVTSITASTHLTVIGTSPHAPGSVRV-HVSMTTANVSWEPGYDGGYEQTFSVWMKRAQFGPHDWLSLSVPPGPSWLLVDSLEPETAYQFSVLAQNRLGTSAF-SEVVTVNTLAFPVTTPEPLVLVTPPRCLTANRT-QQGVLLS 633          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|158706512|sp|Q9UPX0.2|TUTLB_HUMAN (RecName: Full=Protein turtle homolog B; AltName: Full=Immunoglobulin superfamily member 9B; Short=IgSF9B; Flags: Precursor)

HSP 1 Score: 116.701 bits (291), Expect = 5.586e-25
Identity = 127/542 (23.43%), Postives = 227/542 (41.88%), Query Frame = 0
Query:  300 KAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRI-YSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDME-----GRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT-SRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVED-LKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEG------MVKPEDKDVERTISQGIILT 826
            K G    + C T   NP   V W + +G  +   G+ +V  G    +LT       V   D G  + C A + + GE+V+    +              +  P F + P + I +V    D ++     A P ++TY W   Y ++  N+   +D   R+   I   L I   + ED+G+Y    +NS G+S      + V Y A + ++   + V  G   +  C VDA+P  A  + W++ G  ++     G T M++G+    + +   ++E  G + C   N +  M  +    L+++  P     P         R+  ++ C A G P  V +W ++G     +P   +   +                    L  + +S  D+G ++C+A N       + +L V  + P  P  + V   +  +  ++W PG+DGG++Q+F +  ++     + ++ +      S+ + D L+  T Y FSV A N LG S F SE V V TL++         +V P    +     QG++L+
Sbjct:  152 KEGGSITMTC-TAFGNPKPIVTWLK-EGTLLGASGKYQVSDG----SLTVT----SVSREDRGA-YTCRAYSIQ-GEAVHTTHLL--------------VQGPPFIVSPPENI-TVNISQDALLTCRAEAYPGNLTYTW---YWQDE-NVYFQNDLKLRVRILIDGTLIIFRVKPEDSGKYTCVPSNSLGRSPSASAYLTVQYPARVLNMPPVIYVPVGIHGYIRCPVDAEP-PATVVKWNKDGRPLQVEKNLGWTLMEDGS----IRIEEATEEALGTYTCVPYNTLGTMGQSAPARLVLKDPPYFTVLPGWEYRQEAGREL-LIPCAAAGDPFPVITWRKVG-----KPSRSKHSALPS----------------GSLQFRALSKEDHGEWECVATNVVTSITASTHLTVIGTSPHAPGSVRV-QVSMTTANVSWEPGYDGGYEQTFSVWMKRAQFGPHDWLSLPVPPGPSWLLVDTLEPETAYQFSVLAQNKLGTSAF-SEVVTVNTLAFPITTPEPLVLVTPPRCLIANRTQQGVLLS 633          
BLAST of EMLSAG00000004534 vs. SwissProt
Match: gi|49036500|sp|Q9Y6N7.1|ROBO1_HUMAN (RecName: Full=Roundabout homolog 1; AltName: Full=Deleted in U twenty twenty; AltName: Full=H-Robo-1; Flags: Precursor)

HSP 1 Score: 113.62 bits (283), Expect = 4.441e-24
Identity = 152/671 (22.65%), Postives = 259/671 (38.60%), Query Frame = 0
Query:  195 IMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSEL------HLTANYSDNGMVYRCEASN---PATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGE-IRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRI-YSIQN--LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-------FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLV-----RQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTS--------RPDTPTLL-------NVFNYTYNSVYLTWIPGFD-GGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGII 824
            I+ +G+   L C    G P  T+ WY+G E ++ T +DD  +         L      H   +  D G VY C A N    A +  +    + +    R  P  + V         G+ A + C+    +P   + W++ DG  ++++ E I +R G          L I    +     ++C  TN  +GE  ++   +TV            +++P F   P++   +V++ ++       R +P   T RW             D   +P+  Y I++   L I +    D G Y     N  GK+     + V             +V  G T  F+C     P  A  I W R G       +     +S  + + +  LT+ NV + D G ++C   N +A    TK+   +      R  P I + P N   A D   T VL+C A G+P     W + G ++S +  +   +++E+   +I+Y  +                 D G Y CIA    G + ++ Y+ V          RP  P L+        V + + N+V L+W P  + G    S+II      S +       +  T +  ++ LK    Y F V+A N  G S+ +  +  V+T   L      + K V+R +   ++
Sbjct:   78 IVSKGEPATLNC-KAEGRPTPTIEWYKGGERVE-TDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEG-VYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVA-------VGEPAVMECQPPRGHPEPTISWKK-DGSPLDDKDERITIRGG---------KLMITYTRKSDAGKYVCVGTNM-VGERESEVAELTV------------LERPSFVKRPSNLAVTVDDSAEF--KCEARGDPVP-TVRWRK-----------DDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPA--IFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLN-VAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDG--TFVLSCVATGSPVPTILWRKDGVLVSTQ--DSRIKQLENGVLQIRYAKLG----------------DTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVL 678          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: gb|EEC18933.1| (nephrin, putative, partial [Ixodes scapularis])

HSP 1 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 388/909 (42.68%), Postives = 538/909 (59.19%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQ--IDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRN-KIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNE-NIPNITLDSDN--IPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGS-KTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MG T +L C+S GGNPLA++VWYR  E+  +D SY T+GRES N  TFVA+S DNN  YRC A N VS EP++A + L+VQFAP KV + GP EA  GD ++  C T  SNPPA V WVVD R  +   T+ T   +GGW T SNI+V I   +RN K+ SCYA+N  LG+TVV+T  +S+LY P  P I G   GT +     + +TC++  GNPL T+ W++G + +  TT+ +      N   +E+ + A  SDNG VYRCEASNPAT +    L+++I L+V F P  + +K  P  PK+G+K  L CETGSS+P +++ W + DGL +    +  V  GPFGG  + NVL I+V A D    ++C+ATN  L  SV+D +T+            D   KP F   P +  F + EG  LV+N++ RANP  VTY W            T  S +  +P +Y+  +LLN+T   +   G Y  +  NSEG ++  I I++ YGA +  +T+ V+V  G  A  EC VDAKP   DT+SW R GFDM  RT         +LTV+NVS++D+G F C A+NGI + +  K+  L+V+ RP I+ SP + KAA  +  TA L CRAQ  P+  F+WSR GSVI+     D        KY++    +D +T+ES L +K + S DYG Y+C+A+NE G     V+L+  S PD P  L V N TYNS+ L W PGFDGG  QSF +RYRK  S + Y Y DV  S+  ++ +  L   T+Y+  + AFN+ G  E+  + V  +T       V+P       T+   II ++++V   LL LN++L+ C+V++RR  R+      E   ++ S+K+ATIEMYA SSYNETM+GET+SS SEKS  Y+
Sbjct:  185 MGDTTTLRCISRGGNPLAQLVWYRNQEERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPEPVSAAVRLTVQFAPSKVKVRGPKEAKAGDQITMRCSTERSNPPAEVSWVVDGRPFESVSTI-TADPKGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDTVVETAAVSILYAPDAPRIFGYTEGTPIRAKTLQHITCVTNGGNPLPTVRWFKGTQKVNSTTKTNG-----NSVSAEVAIIAQDSDNGAVYRCEASNPATKKA---LTTSIKLTVHFPPTAVTIKIKPRKPKSGQKMTLTCETGSSHPQSSISWWK-DGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTL------------DVTYKPEFYNAPLER-FDIVEGESLVINLTARANPSAVTYSWTKDERRVATARATERSADRALPSVYARGSLLNLTNVERSHGGVYDCEAENSEGSTKTSILINIQYGAIVKAVTETVIVDAGSHAQLECEVDAKPFDEDTVSWRRQGFDM-SRTRQLVKDRRAYLTVHNVSRQDSGPFDCVAHNGIGE-ESVKTANLVVKFRPEIDRSPPHLKAAGQEGGTAELLCRAQAAPNATFAWSREGSVITSSSRPD--------KYDVSVAQVDLVTFESTLSVKAIRSSDYGHYECVARNELGFDSAKVHLDTLSAPDPPLQLQVTNATYNSLTLVWRPGFDGGLPQSFRLRYRKAASNEGYHYQDVLLSNMTNYTIGGLGHDTEYTVGIMAFNEYGQGEYLKDIVKGKTTGECLPTVRPMGSLAVPTV---IITSVSVVGTSLLFLNIVLVICFVRKRRKKRL------EEEESDQSSKTATIEMYAPSSYNETMNGETVSSTSEKSEAYS 1051          

HSP 2 Score: 158.688 bits (400), Expect = 2.100e-38
Identity = 175/691 (25.33%), Postives = 296/691 (42.84%), Query Frame = 0
Query:   52 DNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-----GPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGV-TFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-------VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI--------TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQ------NGTNSMHLTVYNVSK-EDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWS----RMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            D++  Y+C+   T   + + AD  ++V   P  VTI         E    +++   C+ + + P A ++W    V+ R    +++V  ESE G     S++++    +D   + SC A++  L   +  +  + VL+ PG P I G   G  +  G +  L CIS  GNPLA L WYR  E     + D   T +   + + L   AN SDN  VYRC A+N  + +   P+S+ + L+V+F P  + V+  P+  KAG +  + C T  SNP A V W   DG   E+   I   + P GG +TT+ + +++  ++  +  F C A N+ LG++V +   +++       P     D P         IF   EG+ +       +  ++   NP   T RW     + + + T  + N     S+   + I     ++   Y+ + +N   K  L   IK+ V +  T   I        +   M +T ET        + P    +ISW + G  + G            G  S ++ V NV+  +D   ++C A N    +    S  L V  +P    +P       +     + LT RA  +  V +SW+    R+ +  + E   D +            +V  R    S L++ NV     G YDC A+N EG ++ ++ +N+
Sbjct:   32 DDDAEYQCQVGPTSDHKAIRADAHVTVLLPPASVTITDHPNGSSVEVRQSESVKLTCVVSQAKPAAQLKWYRKNVELRPDNVHYSVE-ESEGGRQNAISSLTLTPHSDDNGVVYSCEAVHPALESPMATSVRLGVLFAPGPPEIHGYTEGETIRMGDTTTLRCISRGGNPLAQLVWYRNQE---ERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPE---PVSAAVRLTVQFAPSKVKVRG-PKEAKAGDQITMRCSTERSNPPAEVSWVV-DGRPFESVSTI--TADPKGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDTVVETAAVSI----LYAP-----DAP--------RIFGYTEGTPIRAKTLQHITCVTNGGNPLP-TVRWFKG-TQKVNSTTKTNGN-----SVSAEVAIIAQDSDNGAVYRCEASNPATKKALTTSIKLTVHFPPTAVTIKIKPRKPKSGQKMTLTCETG------SSHP--QSSISWWKDGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTLDVTYKPEFYNAPLERFDIVEGESLVINLTARANPSA-VTYSWTKDERRVATARATERSADRALP----------SVYAR---GSLLNLTNVERSHGGVYDCEAENSEGSTKTSILINI 665          

HSP 3 Score: 104.375 bits (259), Expect = 1.187e-21
Identity = 174/744 (23.39%), Postives = 282/744 (37.90%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQI--DFSYYTT-----GRESR-NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY---RGDEIIQGTTQDDDETETKNYAQSELHLTANYSD---NGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE-VP-KAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWS----RVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVY---LNVTSRPD 709
            ++V L CV     P A++ WYR + ++  D  +Y+      GR++  +  T    S+DN   Y C+A +     P+   + L V FAP    I G TE     +GDT +  CI+   NP A + W  +    QE  +V +     G E+ + ++ +   +D N +  C A N    E V     ++V + P K  + G       D+   R   C +   NP A ++W    R  E +   T D      K    +  ++T N +    N  ++ C A N A       +  T  +S+ + P    +    E  P +A    ++ C T   NP   V W +         G  +V S     GN  +  + I  +  D+G  + CEA+N    +++   I +TV    F    +    KP           + E GS         ++P      W +          +  D      + +N+L I     +D   Y  +  N   +  +   + +DV Y     +   +   +V GE+        A P +A T SW+    RV        S      S++     L + NV +   G + C A N       TK++ L+  Q   I ++        D    A L C     P  +   SW R G  +S+                 +  V DR  +   L + NVS +D G +DC+A N  GI   +V    L V  RP+
Sbjct:   82 ESVKLTCVVSQAKPAAQLKWYRKNVELRPDNVHYSVEESEGGRQNAISSLTLTPHSDDNGVVYSCEAVHPALESPMATSVRLGVLFAPGPPEIHGYTEGETIRMGDTTTLRCISRGGNPLAQLVWYRN----QEERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPEPVSAAVRLTVQFAPSKVKVRGPKEAKAGDQITMR---CSTERSNPPAEVSWVVDGRPFESVSTITADP-----KGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDT---VVETAAVSILYAPDAPRIFGYTEGTPIRAKTLQHITCVTNGGNPLPTVRWFK---------GTQKVNSTTKTNGNSVSAEVAIIAQDSDNGAVYRCEASNPATKKALTTSIKLTVH---FPPTAVTIKIKPRKPKSGQKMTLTCETGS---------SHPQSSISWWKDGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTLDVTYKPEFYNAPLERFDIVEGESLVINLTARANP-SAVTYSWTKDERRVATARATERSADRALPSVYARGSLLNLTNVERSHGGVYDCEAENSEG---STKTSILINIQYGAIVKA-VTETVIVDAGSHAQLECEVDAKPFDEDTVSWRRQGFDMSR----------------TRQLVKDRRAY---LTVHNVSRQDSGPFDCVAHN--GIGEESVKTANLVVKFRPE 763          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: gb|KYB28870.1| (Nephrin-like Protein [Tribolium castaneum])

HSP 1 Score: 642.114 bits (1655), Expect = 0.000e+0
Identity = 357/910 (39.23%), Postives = 540/910 (59.34%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNI-TLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGF-DMEGRTSM---QNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK--PEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTS 903
            GQTV L+C S GGNP A+++WY+  EQI  +Y T GR S N+YTF A + DN  RY+C+A N +S  PL A++ ++V F+P  VTI GPTEA VGD +   C T NSNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I G   G+ +  G  ++++C S  GNPLATLTWY+ D+ I    +  D++ T     +E+ +  N +DN   YRCEA+N AT     PL  TIT++V F P  + ++ +P   K  ++A L C++ SSNP A + W R +G+ +  QG +   + G  GG +++  L +++  + +G+ + C+ATNE L  SV+D IT+ V            + KP+FD D  + +  + EG  L++ +    NP ++ Y W     + +P I +  S  I RI S   +LNIT   + DAG Y  +  NS+G S  ++ I V Y ATI   ++ V+V   E A   C  D  P++ DTI+W R  F D + RTS+   +NGT+ + +T  +V+++D G F C  NNG+ ++  TK   L+V+ +P I++ PA  K ASD   T  + CR+Q +P   + W+R G+ I+              KY   Y  ID +T ES L I +V+S DYG+Y+C+A+NE G +  +  L VTS PDTPTLL + N T+++V L W PGFDGG K ++ IRYR+     Y+YVDV   +  SF + DL++ T+Y FS+ A N LG+S++  + ++  T  Y + + K  PED   ++ + + +I++++++   LLI+N+ L++  + +RR  R+   S       N ++KSATIEMYA SSYN+T++GETLSS+SEKS  Y++
Sbjct:  258 GQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARVGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKINSVLKTTDKSVT-----AEITILTNVTDNEARYRCEAANSAT---EIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAKLSWWR-EGIPV--QGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQV------------LYKPVFDKDNEEAVTGI-EGEPLIITLKADGNPQNIAYTWT---KDGLPIIQSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITVQYAATIVATSENVVVNPNEDATLSCTADGNPLSDDTITWKRDDFPDFDARTSVMYDKNGTSYLRIT--DVTRDDLGNFQCIVNNGVGNVT-TKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAKYKWARSGAPITAN---------TTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELGFATASPRLEVTSAPDTPTLLTILNVTHDTVTLGWTPGFDGGMKAAYRIRYRQVNDDGYKYVDVVPQNATSFLIRDLEVNTQYVFSIMAMNKLGNSKYMPDLLSARTSMYSDHIEKIIPEDLLEKQDLPRIVIISVSVIGIFLLIVNIGLVAGCMLKRRAKRIREQS-------NQTSKSATIEMYAPSSYNDTVTGETLSSVSEKSETYSN 1121          

HSP 2 Score: 123.635 bits (309), Expect = 1.835e-27
Identity = 178/735 (24.22%), Postives = 282/735 (38.37%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQW----VVDSRSAQENFTVRTESERGG-----WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL----VVNISVRA---NPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASD-KRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
             N   +V W +    + FS        Y   G     +Y    S  S +++  Y+C+       + + A+  L+V   P  V I         E         +C   N+ P A + W    V  +   +E+  +    + G      ++T S IS+     D     +C A +  L   +    T+  SV YPPG P I G   G  +  GQ+  L C S  GNP A L WY+  E I+   +         Y       TA+ SDN   Y+CEASN        PL + + ++V F P  + + S P   + G    L C T +SNP A + W    G  + N    R    P GG +TT+ +   VE     +  IC   N +L E+V    T+ V       P   F             I    +GS +    V  IS  +   NP   T  W    N+   N  L + +      I  L N+T    ++  RY+ +  NS  +  L   I ++V +      I  + + +   E A   C   +    A  +SW R G  ++G     +  +  GT S      N++++  G  + C A N         +  L V  +P  ++   N +A +  + +  ++T +A G P ++ ++W++ G  I Q       E I  +                 L+I  +S  D G+Y C A N +G S   V + V
Sbjct:   47 ANLAGQVQWTKDGFALGFSSVIPGFPRYSVIGDRRHGIYNLRVSNASLEDDAEYQCQVGPARLHKAIRANARLNVISPPSSVEILDHRHNSKIEIKENQEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYT-----FTADASDNKARYKCEASN---IMSKAPLKAEVDMTVLFSPAHVTI-SGPTEARVGDPVPLTCTTANSNPPAEIKWMV-AGRQVRNATS-RTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINV----LYPPSAPF-------------IHGYTQGSYIPAGTVQKISCTSSGGNPL-ATLTWYK--NDKKINSVLKTTDKSVTAEITILTNVT----DNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAK-LSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKD--NEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDG--------------PVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITV 729          

HSP 3 Score: 92.4337 bits (228), Expect = 6.027e-18
Identity = 170/755 (22.52%), Postives = 288/755 (38.15%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDF---------------SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE---VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATI-SDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSM-----HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRPDT---PTLL 714
            Q   L C      P A++VWYRG+ +++                    T  ++ +  +   ++ED+   Y C+A++       PL   + LSV + P    IEG TE      G T+   C +   NPPA + W  +         +R      G  + +  +     +D      C A N      +     ++VL+ P   +I+G     +   G    LTC +   NP A + W   G ++   T++     E      S +      +   +V  C   N    Q +  + ST T++V + P    +    +   +P AG    + C +   NP A + W +ND   I +  +   +S      + TNV   D EAR     + CEA N      + + ITM V    +  P    I K   ++ P        E + L  + S    P  +++     + E IP   L +   P ++     SI+  LNITE  + +   Y  +  N   +  +   I + VLY      D  +AV  + GE        D  P      +W++ G  +  ++S  +G   +      L +  +S+ DAG + C A N  +         + V+    I  +  N     +  + A L+C A G P  D   +W R              ++  D  ++ + +V+      S L I +V+  D G++ CI  N  G ++   V L V  +P+    P LL
Sbjct:  145 QEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQ------IRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARV---GDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLN---MQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIP-AGTVQKISCTSSGGNPLATLTWYKNDK-KINSVLKTTDKSVTAEITILTNV--TDNEAR-----YRCEAANSATEIPLFETITMNV----YFPPDHVKIRKEPLELKP-------NEQATLTCDSSSSNPPAKLSW-----WREGIPVQGLLNTTKPGLHGGTVSSIELKLNITE--QLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNI-AYTWTKDGLPII-QSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALN--SQGSSVAQVNITVQYAATIVATSEN--VVVNPNEDATLSCTADGNPLSDDTITWKR--------------DDFPD--FDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALL 838          

HSP 4 Score: 88.5817 bits (218), Expect = 8.950e-17
Identity = 145/616 (23.54%), Postives = 237/616 (38.47%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG---TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKA--YLFCETGSSNPTANVIWRRNDGLSI----ENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQN---LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV---TGETAHFECVVDAKPITADTISWSRVGFDMEGRTS---------MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            +G  V L C +   NP AE+ W     Q+  +   T      G  + +  T V      +    C   N    E + +   ++V + P    I G T+ +    G      C ++  NP A + W  + +    N  ++T  +       + I++L    D      C A N+     + +T T++V +PP    I   +P  +    Q+  LTC S + NP A L+W+R    +QG   TT+      T +  + +L++T     NG+VY C+A+N A  +    +   ITL V +KP+      D E    G +    +       NP         DGL I     + G  R+ S GP        VL I   +R    T+ CEA N + G SV   + +TV+  +               I  T     V    D  ++ +   NP  D T  W     ++ P    D D    +   +N    L IT+  ++D G ++  VNN  G    K  + ++      D   A++      G+T    C   A P+      W+R G  +   T+         +   T+   L + +V+  D G + C A N + 
Sbjct:  351 VGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKI--NSVLKTTDK----SVTAEITILTNVTDNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRK-EPLELKPNEQA-TLTCDSSSSNPPAKLSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQL--NGIVYTCQATNEALQRS---VHDAITLQVLYKPV---FDKDNEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDGP--------VLNITRLSRHDAGTYSCEALNSQ-GSSVAQ-VNITVQYAA--------------TIVATSENVVVNPNEDATLSCTADGNPLSDDTITWK---RDDFP----DFDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAK--YKWARSGAPITANTTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELG 917          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: AHN56011.1 (sticks and stones, isoform C [Drosophila melanogaster])

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 348/912 (38.16%), Postives = 514/912 (56.36%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET---------LSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            GQTV L+C S GGNP A+++WY+   QI  +Y T+GR S N+YTF A + DN  R+RC+A N +S  PL A++ LSV FAP  VT+ GPTEA VGD +   C T  SNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I+G   G I+  G  ++L C+S  GNPLATLTWY+ D+ I    +  D++       +E+ + AN SDN   YRCEASN AT     PL  + TLSV F P T+ ++ +PE  + G +A + C++ SSNP A + W + DG+ IE       + G +GG ++T    ++V    +G  + C++ NE L  S ++ +++            D + +P F   P+ T   V EG  L V++  RANP  VTY+W           T+  D   RI++    LN T   ++DAG Y    +NS+G + L I + V YG TI  +++ ++V  GE A   C V+ KP+T + + W RVG+DM  +TS    NGT+ +H  + +  +ED G F C A+N + D    +   L+V+  P I ++P   +AAS   +   L CRAQG+P   F W +         ++ +       KYE++   ID +T+ES L +  V+  DYG+Y+C+A+NE G +   V L +TS+PD P  LN+ N T+++V L W PGFDGG K S+ +RYR    + Y+Y+D   +S +   +  L++ T Y FSV ++N+LG S +  +    ET          S L G   P         + G++L       ++++LNV +I C + +R   R+    VAE  +TN   K+ATIEMYA SSYN+T++GETLSS+SEKS  Y+
Sbjct:  300 GQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKS-----VSAEITILANVSDNQAQYRCEASNSATEI---PLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAKLSWWK-DGIPIEGINNTS-KPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSL------------DVLYRPKFVPPPSSTAVGV-EGESLQVSLQTRANPTPVTYKWTK------DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLH--IKDAKREDVGNFRCVADNRV-DNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQ---------DKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNS-HKLTIGGLRMNTLYLFSVMSWNELGQSSYLPDLARAETKEAPPPSHPASSLGGG-PPTTSQTPLGGTSGMLLVGVGAGIVVVLLNVFVIGCCLHKRNEKRLK--RVAE--TTNQPAKTATIEMYAPSSYNDTVTGETLSSVSEKSESYS 1164          

HSP 2 Score: 137.887 bits (346), Expect = 7.464e-32
Identity = 177/723 (24.48%), Postives = 276/723 (38.17%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLID-FIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGA-TISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT 705
             N    V W +    + FS        Y   G   + +Y    S  S +++  Y+C+         + A+  L+V   P  + I+G       E      L   CI  N+ P A + W    V+ +  +   TV  ES    + T S++ +  GP+D     +C A +  L   +    T+  SVLYPPG P I G   G  +  GQ+  L C S  GNP A L WY+    I+   +         Y       TA   DN   +RCEASN        PL + + LSV F P  + V    E  + G    L C T  SNP A + W    G  + N     + S P GG  TT+ +   VE     +  IC   N +L E+V    T+ V       PLI  +++  I        +  V  G           NP      + N  ++ I ++   +D      S+   + I     ++  +Y+ + +NS  +  L     + V +   T+    +   +  G  A   C   +    A  +SW + G  +EG     +  +  GT S      NV++E  G+ + C + N        ++  L V  RP+    P+++    +     V L  RA  TP V + W++ G+ I Q    D    I  +   + +  + R               D G Y C A N +G       LN+T
Sbjct:   96 ANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVE-ESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYT-----FTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAPTHVTVMGPTEA-RVGDIVPLTCTTAPSNPPAEIKWMVG-GRQVRNATSKTIVS-PEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPP-APPLISGYMEGQIIPAGSVQKLLCVSSG----------GNPLATLTWYKN--DKRINSVIRAADK-----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAK-LSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTP-VTYKWTKDGTTIPQ----DGDHRIFADGGSLNFTRLHR--------------DDAGIYSCSASNSQG----GATLNIT 764          

HSP 3 Score: 94.3597 bits (233), Expect = 1.805e-18
Identity = 179/833 (21.49%), Postives = 314/833 (37.70%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASS--------EDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDN--GMVYRCEASNPATAQESGPLSSTITLSVRFKPI--TIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMT-WESELHIKNVSSRDYGSYDCIAQNE-EGISRYAVYLNVTSRPD---TPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKG---SKTYQYVDVHHSSTNSFEVEDLKLGTKY------SFSVKAFNDLGDS 789
            Q + L C+     P A++VWYRG+ +          E      F  +S        +D+   Y C+A++       P+ A + LSV + P    IEG +       G T+   C +   NPPA + W  +   +Q     RT           NI         NK    C A N      +     +SVL+ P   ++ G     +   G    LTC +   NP A + W  G   ++  T     +    +  +  ++TA    N   +V  C   N    Q +  + ST T++V + P    I+   + ++  AG    L C +   NP A + W +ND         IR         +T     I     D+   + CEA+N      +    T++V           F  + +   I+P +    +E  + ++ + S    P  +++     + + IP   +++ + P ++     +++  +N+T+         +      +  +   + +DVLY         +  V V GE+        A P T  T  W++     +G T  Q+G + +      L    + ++DAG + C+A+N  +    T +  ++V     I+    N     +  + A+L+C  +G P  +    W R+G                   Y++            S LHIK+    D G++ C+A N  +  +   + L V   P+   TPTLL   + T     L       G  K  FI R  KK    ++TY+Y +V     +S   E   +  K       ++   A N+LG++
Sbjct:  194 QDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKN--GSQIRMAYRTSG-----RLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARV---GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLN---MQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK---RINSVIRAADKSVSAEIT-----ILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH----------FAPETVKIRIEPEELRPGME--ATIICDSSSSNPPAKLSW-----WKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANP-TPVTYKWTK-----DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASN--SQGGATLNITVVVEYGTTIKSVSEN--IVVNPGEDAMLSCTVEGKPLTEEHVKWERVG-------------------YDMTVKTSTTFANGTSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPC--RAQGSPKPQFIWRQDKKDLPINRTYKY-EVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA 955          

HSP 4 Score: 90.8929 bits (224), Expect = 2.276e-17
Identity = 130/611 (21.28%), Postives = 238/611 (38.95%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTF---VASSEDNNKRYR---CKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQS-ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTY-RWAN-PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS-EGKSRLKIKIDVLYGATISDITDAVMVV--TGETAHFECVVDAKPITADTISWSR----------VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT 586
            +G  V L C +   NP AE+ W  G  Q+  +   T       +T    + +  + NKR     C   N    E + +   ++V + P    I G  E  +   G      C+++  NP A + W  + +  + N  +R   +       + I++L   +D      C A N+     + Q+ T+SV + P    I  I+P   +  G    + C S + NP A L+W++    I+G               + E  +      NG VY C+++N A  + +      ++L V ++P  +   S   V   G+   +  +T  +NPT        DG +I   G+ R+ +   GG+L    L      RD    + C A+N + G ++N  IT+ V+  + +  + + I               V  G D +++ +V   P    + +W    Y+  +   T  ++         + L+I +A++ED G ++   +N  +  +   I + V +   I+     +     TGE     C     P       W +            +++E R  + + T    L V  V+  D G + C A N + +  +T
Sbjct:  393 VGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK--RINSVIRAADK----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIR-IEPEE-LRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSA---HEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQT-RANPTPVTYKWTKDGTTIPQDGDHRIFAD--GGSLNFTRL-----HRDDAGIYSCSASNSQGGATLN--ITVVVEYGTTIKSVSENI--------------VVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGT-------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSP--KPQFIWRQDKKDLPINRTYKYEVEER-KIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVET 958          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: AAF59040.2 (sticks and stones, isoform A [Drosophila melanogaster])

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 349/904 (38.61%), Postives = 516/904 (57.08%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISC-YVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            GQTV L+C S GGNP A+++WY+   QI  +Y T+GR S N+YTF A + DN  R+RC+A N +S  PL A++ LSV FAP  VT+ GPTEA VGD +   C T  SNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I+G   G I+  G  ++L C+S  GNPLATLTWY+ D+ I    +  D++       +E+ + AN SDN   YRCEASN AT     PL  + TLSV F P T+ ++ +PE  + G +A + C++ SSNP A + W + DG+ IE       + G +GG ++T    ++V    +G  + C++ NE L  S ++ +++            D + +P F   P+ T   V EG  L V++  RANP  VTY+W           T+  D   RI++    LN T   ++DAG Y    +NS+G + L I + V YG TI  +++ ++V  GE A   C V+ KP+T + + W RVG+DM  +TS    NGT+ +H  + +  +ED G F C A+N + D    +   L+V+  P I ++P   +AAS   +   L CRAQG+P   F W +         ++ +       KYE++   ID +T+ES L +  V+  DYG+Y+C+A+NE G +   V L +TS+PD P  LN+ N T+++V L W PGFDGG K S+ +RYR    + Y+Y+D   +S +   +  L++ T Y FSV ++N+LG S +  +    ET   LE M     +D   T +  +I+ I+L +   L++N  L++  +V +RR        VAE  +TN   K+ATIEMYA SSYN+T++GETLSS+SEKS  Y+
Sbjct:  300 GQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKS-----VSAEITILANVSDNQAQYRCEASNSATEI---PLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAKLSWWK-DGIPIEGINNTS-KPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSL------------DVLYRPKFVPPPSSTAVGV-EGESLQVSLQTRANPTPVTYKWTK------DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLH--IKDAKREDVGNFRCVADNRV-DNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQ---------DKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNS-HKLTIGGLRMNTLYLFSVMSWNELGQSSYLPDLARAETKG-LELMPAELTEDSSNTPNL-VIIGISLAAFGFLLVNASLVAWFFVHQRR------KKVAE--TTNQPAKTATIEMYAPSSYNDTVTGETLSSVSEKSESYS 1151          

HSP 2 Score: 137.502 bits (345), Expect = 1.070e-31
Identity = 177/723 (24.48%), Postives = 276/723 (38.17%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLID-FIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGA-TISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT 705
             N    V W +    + FS        Y   G   + +Y    S  S +++  Y+C+         + A+  L+V   P  + I+G       E      L   CI  N+ P A + W    V+ +  +   TV  ES    + T S++ +  GP+D     +C A +  L   +    T+  SVLYPPG P I G   G  +  GQ+  L C S  GNP A L WY+    I+   +         Y       TA   DN   +RCEASN        PL + + LSV F P  + V    E  + G    L C T  SNP A + W    G  + N     + S P GG  TT+ +   VE     +  IC   N +L E+V    T+ V       PLI  +++  I        +  V  G           NP      + N  ++ I ++   +D      S+   + I     ++  +Y+ + +NS  +  L     + V +   T+    +   +  G  A   C   +    A  +SW + G  +EG     +  +  GT S      NV++E  G+ + C + N        ++  L V  RP+    P+++    +     V L  RA  TP V + W++ G+ I Q    D    I  +   + +  + R               D G Y C A N +G       LN+T
Sbjct:   96 ANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVE-ESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYT-----FTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAPTHVTVMGPTEA-RVGDIVPLTCTTAPSNPPAEIKWMVG-GRQVRNATSKTIVS-PEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPP-APPLISGYMEGQIIPAGSVQKLLCVSSG----------GNPLATLTWYKN--DKRINSVIRAADK-----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAK-LSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTP-VTYKWTKDGTTIPQ----DGDHRIFADGGSLNFTRLHR--------------DDAGIYSCSASNSQG----GATLNIT 764          

HSP 3 Score: 93.5893 bits (231), Expect = 2.773e-18
Identity = 179/833 (21.49%), Postives = 314/833 (37.70%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASS--------EDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDN--GMVYRCEASNPATAQESGPLSSTITLSVRFKPI--TIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMT-WESELHIKNVSSRDYGSYDCIAQNE-EGISRYAVYLNVTSRPD---TPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKG---SKTYQYVDVHHSSTNSFEVEDLKLGTKY------SFSVKAFNDLGDS 789
            Q + L C+     P A++VWYRG+ +          E      F  +S        +D+   Y C+A++       P+ A + LSV + P    IEG +       G T+   C +   NPPA + W  +   +Q     RT           NI         NK    C A N      +     +SVL+ P   ++ G     +   G    LTC +   NP A + W  G   ++  T     +    +  +  ++TA    N   +V  C   N    Q +  + ST T++V + P    I+   + ++  AG    L C +   NP A + W +ND         IR         +T     I     D+   + CEA+N      +    T++V           F  + +   I+P +    +E  + ++ + S    P  +++     + + IP   +++ + P ++     +++  +N+T+         +      +  +   + +DVLY         +  V V GE+        A P T  T  W++     +G T  Q+G + +      L    + ++DAG + C+A+N  +    T +  ++V     I+    N     +  + A+L+C  +G P  +    W R+G                   Y++            S LHIK+    D G++ C+A N  +  +   + L V   P+   TPTLL   + T     L       G  K  FI R  KK    ++TY+Y +V     +S   E   +  K       ++   A N+LG++
Sbjct:  194 QDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKN--GSQIRMAYRTSG-----RLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARV---GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLN---MQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK---RINSVIRAADKSVSAEIT-----ILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH----------FAPETVKIRIEPEELRPGME--ATIICDSSSSNPPAKLSW-----WKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANP-TPVTYKWTK-----DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASN--SQGGATLNITVVVEYGTTIKSVSEN--IVVNPGEDAMLSCTVEGKPLTEEHVKWERVG-------------------YDMTVKTSTTFANGTSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPC--RAQGSPKPQFIWRQDKKDLPINRTYKY-EVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA 955          

HSP 4 Score: 90.5077 bits (223), Expect = 3.001e-17
Identity = 130/611 (21.28%), Postives = 238/611 (38.95%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTF---VASSEDNNKRYR---CKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQS-ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTY-RWAN-PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS-EGKSRLKIKIDVLYGATISDITDAVMVV--TGETAHFECVVDAKPITADTISWSR----------VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT 586
            +G  V L C +   NP AE+ W  G  Q+  +   T       +T    + +  + NKR     C   N    E + +   ++V + P    I G  E  +   G      C+++  NP A + W  + +  + N  +R   +       + I++L   +D      C A N+     + Q+ T+SV + P    I  I+P   +  G    + C S + NP A L+W++    I+G               + E  +      NG VY C+++N A  + +      ++L V ++P  +   S   V   G+   +  +T  +NPT        DG +I   G+ R+ +   GG+L    L      RD    + C A+N + G ++N  IT+ V+  + +  + + I               V  G D +++ +V   P    + +W    Y+  +   T  ++         + L+I +A++ED G ++   +N  +  +   I + V +   I+     +     TGE     C     P       W +            +++E R  + + T    L V  V+  D G + C A N + +  +T
Sbjct:  393 VGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK--RINSVIRAADK----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIR-IEPEE-LRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSA---HEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQT-RANPTPVTYKWTKDGTTIPQDGDHRIFAD--GGSLNFTRL-----HRDDAGIYSCSASNSQGGATLN--ITVVVEYGTTIKSVSENI--------------VVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGT-------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSP--KPQFIWRQDKKDLPINRTYKYEVEER-KIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVET 958          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: ABI31083.1 (sticks and stones, isoform B [Drosophila melanogaster])

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 349/958 (36.43%), Postives = 516/958 (53.86%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET---------LSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMN-----------------------GSSVAEGG-----------------------STNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            GQTV L+C S GGNP A+++WY+   QI  +Y T+GR S N+YTF A + DN  R+RC+A N +S  PL A++ LSV FAP  VT+ GPTEA VGD +   C T  SNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I+G   G I+  G  ++L C+S  GNPLATLTWY+ D+ I    +  D++       +E+ + AN SDN   YRCEASN AT     PL  + TLSV F P T+ ++ +PE  + G +A + C++ SSNP A + W + DG+ IE       + G +GG ++T    ++V    +G  + C++ NE L  S ++ +++            D + +P F   P+ T   V EG  L V++  RANP  VTY+W           T+  D   RI++    LN T   ++DAG Y    +NS+G + L I + V YG TI  +++ ++V  GE A   C V+ KP+T + + W RVG+DM  +TS    NGT+ +H  + +  +ED G F C A+N + D    +   L+V+  P I ++P   +AAS   +   L CRAQG+P   F W +         ++ +       KYE++   ID +T+ES L +  V+  DYG+Y+C+A+NE G +   V L +TS+PD P  LN+ N T+++V L W PGFDGG K S+ +RYR    + Y+Y+D   +S +   +  L++ T Y FSV ++N+LG S +  +    ET          S L G   P         + G++L       ++++LNV +I C + +R   R+                        G S+A  G                       +TN   K+ATIEMYA SSYN+T++GETLSS+SEKS  Y+
Sbjct:  300 GQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKS-----VSAEITILANVSDNQAQYRCEASNSATEI---PLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAKLSWWK-DGIPIEGINNTS-KPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSL------------DVLYRPKFVPPPSSTAVGV-EGESLQVSLQTRANPTPVTYKWTK------DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLH--IKDAKREDVGNFRCVADNRV-DNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQ---------DKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNS-HKLTIGGLRMNTLYLFSVMSWNELGQSSYLPDLARAETKEAPPPSHPASSLGGG-PPTTSQTPLGGTSGMLLVGVGAGIVVVLLNVFVIGCCLHKRNEKRLKRGLELMPAELTEDSSNTPNLVIIGISLAAFGFLLVNASLVAWFFVHQRRKKVAETTNQPAKTATIEMYAPSSYNDTVTGETLSSVSEKSESYS 1214          

HSP 2 Score: 137.887 bits (346), Expect = 8.594e-32
Identity = 177/723 (24.48%), Postives = 276/723 (38.17%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLID-FIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGA-TISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT 705
             N    V W +    + FS        Y   G   + +Y    S  S +++  Y+C+         + A+  L+V   P  + I+G       E      L   CI  N+ P A + W    V+ +  +   TV  ES    + T S++ +  GP+D     +C A +  L   +    T+  SVLYPPG P I G   G  +  GQ+  L C S  GNP A L WY+    I+   +         Y       TA   DN   +RCEASN        PL + + LSV F P  + V    E  + G    L C T  SNP A + W    G  + N     + S P GG  TT+ +   VE     +  IC   N +L E+V    T+ V       PLI  +++  I        +  V  G           NP      + N  ++ I ++   +D      S+   + I     ++  +Y+ + +NS  +  L     + V +   T+    +   +  G  A   C   +    A  +SW + G  +EG     +  +  GT S      NV++E  G+ + C + N        ++  L V  RP+    P+++    +     V L  RA  TP V + W++ G+ I Q    D    I  +   + +  + R               D G Y C A N +G       LN+T
Sbjct:   96 ANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVE-ESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYT-----FTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAPTHVTVMGPTEA-RVGDIVPLTCTTAPSNPPAEIKWMVG-GRQVRNATSKTIVS-PEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPP-APPLISGYMEGQIIPAGSVQKLLCVSSG----------GNPLATLTWYKN--DKRINSVIRAADK-----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAK-LSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTP-VTYKWTKDGTTIPQ----DGDHRIFADGGSLNFTRLHR--------------DDAGIYSCSASNSQG----GATLNIT 764          

HSP 3 Score: 93.9745 bits (232), Expect = 2.417e-18
Identity = 179/833 (21.49%), Postives = 314/833 (37.70%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASS--------EDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDN--GMVYRCEASNPATAQESGPLSSTITLSVRFKPI--TIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMT-WESELHIKNVSSRDYGSYDCIAQNE-EGISRYAVYLNVTSRPD---TPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKG---SKTYQYVDVHHSSTNSFEVEDLKLGTKY------SFSVKAFNDLGDS 789
            Q + L C+     P A++VWYRG+ +          E      F  +S        +D+   Y C+A++       P+ A + LSV + P    IEG +       G T+   C +   NPPA + W  +   +Q     RT           NI         NK    C A N      +     +SVL+ P   ++ G     +   G    LTC +   NP A + W  G   ++  T     +    +  +  ++TA    N   +V  C   N    Q +  + ST T++V + P    I+   + ++  AG    L C +   NP A + W +ND         IR         +T     I     D+   + CEA+N      +    T++V           F  + +   I+P +    +E  + ++ + S    P  +++     + + IP   +++ + P ++     +++  +N+T+         +      +  +   + +DVLY         +  V V GE+        A P T  T  W++     +G T  Q+G + +      L    + ++DAG + C+A+N  +    T +  ++V     I+    N     +  + A+L+C  +G P  +    W R+G                   Y++            S LHIK+    D G++ C+A N  +  +   + L V   P+   TPTLL   + T     L       G  K  FI R  KK    ++TY+Y +V     +S   E   +  K       ++   A N+LG++
Sbjct:  194 QDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKN--GSQIRMAYRTSG-----RLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARV---GDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTS-NITAVVEPNKRSLVVICHGLN---MQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK---RINSVIRAADKSVSAEIT-----ILANVSDNQAQYRCEASNSATEIPLFQSTTLSVH----------FAPETVKIRIEPEELRPGME--ATIICDSSSSNPPAKLSW-----WKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANP-TPVTYKWTK-----DGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASN--SQGGATLNITVVVEYGTTIKSVSEN--IVVNPGEDAMLSCTVEGKPLTEEHVKWERVG-------------------YDMTVKTSTTFANGTSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPC--RAQGSPKPQFIWRQDKKDLPINRTYKY-EVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA 955          

HSP 4 Score: 90.5077 bits (223), Expect = 2.381e-17
Identity = 130/611 (21.28%), Postives = 238/611 (38.95%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTF---VASSEDNNKRYR---CKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQS-ELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTY-RWAN-PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS-EGKSRLKIKIDVLYGATISDITDAVMVV--TGETAHFECVVDAKPITADTISWSR----------VGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDT 586
            +G  V L C +   NP AE+ W  G  Q+  +   T       +T    + +  + NKR     C   N    E + +   ++V + P    I G  E  +   G      C+++  NP A + W  + +  + N  +R   +       + I++L   +D      C A N+     + Q+ T+SV + P    I  I+P   +  G    + C S + NP A L+W++    I+G               + E  +      NG VY C+++N A  + +      ++L V ++P  +   S   V   G+   +  +T  +NPT        DG +I   G+ R+ +   GG+L    L      RD    + C A+N + G ++N  IT+ V+  + +  + + I               V  G D +++ +V   P    + +W    Y+  +   T  ++         + L+I +A++ED G ++   +N  +  +   I + V +   I+     +     TGE     C     P       W +            +++E R  + + T    L V  V+  D G + C A N + +  +T
Sbjct:  393 VGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDK--RINSVIRAADK----SVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIR-IEPEE-LRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSA---HEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQT-RANPTPVTYKWTKDGTTIPQDGDHRIFAD--GGSLNFTRL-----HRDDAGIYSCSASNSQGGATLN--ITVVVEYGTTIKSVSENI--------------VVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGT-------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSP--KPQFIWRQDKKDLPINRTYKYEVEER-KIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVET 958          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: gb|KFM57797.1| (Nephrin, partial [Stegodyphus mimosarum])

HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0
Identity = 321/754 (42.57%), Postives = 464/754 (61.54%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRN-KIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGD-EIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDN--IPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDM-EGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVI--SQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKK 747
            MG T++L CVS GGNPLA++VWYR D+Q+D +Y TTGRES N  TFV  S DNN  YRC+A N+VS++P+ A + L+VQFAP KVT+ GP EA  GDT++  C T  SNPPA V WVV+ RS Q   ++ T   RGGW T SN++  I  ++RN K+ SCYA+N  L ET+V++  +SVLYPP  P+I G   GT ++ GQ++++TCIS  GNPL  L W+R D E++  TT ++      N   +E  +T + +DNG+ YRCEASN A+   + P+ +T  L+V F P  + +K  P+ PKAG+K  L CE  SSNP A + W + DG  I+   +  V   P GG  T N+L ++V A D G  + C+ATN EL +S +D +T            I+ + KP+F+  P    + V EG   ++NIS R NP  + Y   +  + +   +  DS++  I  + +   L N+T  R++ AG+Y+ + +N EG S + + ++VLY A+IS  T+  MV  G  A  EC VDA P++ D I W R+ FDM   R SM+NG +  +L + NV++ED+G FLC A+NGI +     ST L+V+ +P IE + A SKAA  + +   + CRA G P+V F+W R GS I  S++P           KY+IK   +D +++ES L +K++ ++D+G Y+CIA+N+ G   + + L   S PD PT L+V N T+N+V LTW PGFDGG  Q + I+Y+K+
Sbjct:   29 MGDTLTLACVSRGGNPLAKLVWYRNDQQVDVTYTTTGRESTNTLTFVVESNDNNAIYRCEATNSVSLKPMIAMVKLTVQFAPSKVTLVGPKEAQEGDTVTMFCTTDRSNPPADVSWVVEGRSVQAATSI-TADPRGGWITTSNVTYTISGHERNMKMFSCYAVNKALSETLVESAVVSVLYPPEPPTILGYTEGTPIETGQTQQMTCISQGGNPLPNLKWFRKDKELVSSTTTNN------NIVSAESSITVDDNDNGVEYRCEASNSAS---NVPMFATTRLTVYFPPSAVNIKIKPKRPKAGQKVTLICECASSNPQAEITWWK-DGFLIQGTPD-GVFDAPNGGRSTKNILQLNVSAEDDGAVYTCQATNTELKQSAHDAVT------------INVLYKPVFETKPLQQ-YDVIEGESTIINISARGNPSTINYS-WSKASSSTSQMPADSNDVAITSVLATGPLFNMTNVRRDQAGQYRLEASNEEGSSFVTVVVNVLYAASISMATEVAMVDLGSNAFLECQVDANPLSDDVIRWQRMNFDMSRTRQSMENGRS--YLIINNVTREDSGIFLCVADNGIGNEAQKAST-LVVKHKPVIERAGAYSKAAGSEGEDVRIVCRATGAPNVSFTWFRDGSTIGGSKKP----------SKYDIKSRHLDFISYESVLTVKDLKTQDFGDYECIAKNDLGFESFKIPLGRKSVPDPPTSLSVINATHNAVILTWTPGFDGGLDQMYRIQYKKE 743          

HSP 2 Score: 109.383 bits (272), Expect = 2.242e-23
Identity = 128/540 (23.70%), Postives = 228/540 (42.22%), Query Frame = 0
Query:  176 ISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGV-TFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGR-----TSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            +SVL+ PG P I G   G  +  G +  L C+S  GNPLA L WYR D+ +     D   T T   + + L      +DN  +YRCEA+N  + +   P+ + + L+V+F P  + +   P+  + G    +FC T  SNP A+V W   +G S+  Q    + + P GG +TT+ +   +   +  +  F C A N+ L E++ +   ++V        ++ + +    +   T  +  + +G + + N+           +W     E + + T +++    I S ++  +IT    ++   Y+ + +NS     +    ++ V +  +  +I         G+     C   +    A+ I+W + GF ++G       +   G ++ ++   NVS ED G  + C A N         +  + V  +P  E  P       +   T ++   A+G P  + +SWS+  S  SQ P +     I              +      ++ NV     G Y   A NEEG S   V +NV
Sbjct:    7 LSVLFSPGPPEIEGYVEGETVRMGDTLTLACVSRGGNPLAKLVWYRNDQQV-----DVTYTTTGRESTNTLTFVVESNDNNAIYRCEATNSVSLK---PMIAMVKLTVQFAPSKVTLVG-PKEAQEGDTVTMFCTTDRSNPPADVSWVV-EGRSV--QAATSITADPRGGWITTSNVTYTISGHERNMKMFSCYAVNKALSETLVESAVVSVLYPPEPPTILGYTEGTPIETGQTQQMTCISQGGNPLPNL-----------KWFRKDKELVSSTTTNNN----IVSAES--SITVDDNDNGVEYRCEASNSASNVPMFATTRLTVYFPPSAVNIKIKPKRPKAGQKVTLICECASSNPQAE-ITWWKDGFLIQGTPDGVFDAPNGGRSTKNILQLNVSAEDDGAVYTCQATNTELKQSAHDAVTINVLYKPVFETKPLQQYDVIEGEST-IINISARGNPSTINYSWSKASSSTSQMPADSNDVAI-----------TSVLATGPLFNMTNVRRDQAGQYRLEASNEEGSSFVTVVVNV 504          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: EEB10837.1 (Nephrin precursor, putative [Pediculus humanus corporis])

HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 349/915 (38.14%), Postives = 526/915 (57.49%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISW-SRVGFDMEGRTSMQNGTNSM-HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSW--SRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKT----YQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVD 907
            GQT+ LIC S GGNP A+++W++  E +  SY T GR S N+YTF A S DN  +Y+C+A N +S  P  A++ LSV FAP  VTI GPTEA  GD +   C T NSNPPA ++W+V  R  +    + T S  GG  + SNI+V +G N R+ ++ C+ +N  L E +V THTI+VLYPPG P I+G   GT +  G  + ++CIS  GNPLATLTWY+ D+ I   ++ +D +       +E+ +  N +DNG VY+CEASNPAT     P   T+ L+V F P  + V+ +P   K G+ A L CE+ SSNP A + W + +G+ +   G I   + G  GG ++T  L ++V    +GV + C+ATN  L  SV+D  T+            D + KP+F  + T+ I+S  EG  L++ +  +ANP ++ Y W    N+N   ++    N  R+     +LNIT   + D G YK   +NS+G + +   I V Y A +      V+   GE     C VD  P+  D ISW ++  +D++ +T  +   NS  +L + + +++D+G F C  NNGI + +  K+  LLV+ +P ++ SPA  K+AS+   T  L CR+   P   F W  +RM  V +              KY  ++  ++  T+ES L I NV + DYG Y+C A+NEEGI++  + LNVTS PD P  + V N T++SV ++W+PGFDGG K S+ IRY+     +    ++Y+DV   ++  + V  L L T+Y F++ AFND G+S +  + V  +T      ++ PE+   +  I + II+++++   +LL+ NV+L+ C++ R++        + +G S  GS KSATIEMYA SSYNET++GETLSS+SEKS  Y++  V+
Sbjct:  207 GQTIELICRSRGGNPPAQLIWFKNGEPVRMSYRTAGRLSENVYTFTADSNDNKAKYKCEATNVMSPIPYKAEVTLSVLFAPAHVTISGPTEAKAGDKVPLTCTTDNSNPPADIKWMVAGRQVRNTSVITTPSPNGGSISTSNITVTVGQNKRSLVVICHGLNMHLTENIVGTHTINVLYPPGHPIISGYTKGTNIPVGTVQMISCISSGGNPLATLTWYKNDKKITSVSKTNDRS-----VSAEITVYTNMTDNGAVYKCEASNPATEV---PFIETVKLNVYFPPDHVRVRKEPAELKPGQIATLTCESSSSNPPAKLSWWK-EGIPV--GGAINTTKPGLHGGVISTIELKVNVTPEINGVVYTCQATNAPLQRSVHDAFTL------------DVLFKPVFK-ELTNKIYSAVEGEPLLIFLETKANPSNIVYTW----NKN--GVSFSKKN-SRLIVDGPVLNITRLTRHDNGEYKCTASNSQGSASITFNISVQYSAYVVTAPQYVVATPGENVELNCTVDGNPLKEDDISWRNKNYYDLDEKTISKTFINSTSYLVLRSPTRKDSGSFECVVNNGIGN-ETFKTIELLVKYKPEMDFSPARLKSASNIGSTGRLICRSSSVPKPTFQWFRNRMNLVPNI-----------TSKYFSEFREVNFTTFESILIINNVEASDYGEYECEARNEEGIAKSIIVLNVTSAPDPPLSMTVLNVTHDSVTISWVPGFDGGLKTSYRIRYKADNKNSEREGFRYLDVLTDNSTYYTVNGLNLDTEYVFTIMAFNDRGNSNYMQDVVKTKT----SNIMIPEELLGKADIPRIIIISVSITGVILLLFNVLLVVCFIIRKK-----KKKIKKGPSEQGSNKSATIEMYAPSSYNETVTGETLSSVSEKSENYSNGEVN 1069          

HSP 2 Score: 122.865 bits (307), Expect = 2.649e-27
Identity = 163/660 (24.70%), Postives = 270/660 (40.91%), Query Frame = 0
Query:   53 NNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNI--SVLIGPNDRNKII--SCYAINNDL--GETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGAT-ISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG 694
            NNK  R  A+ TV   P + +I+     + DK+ I+   E      L  +C+  NS PPA V W    ++ +S   + TV    E  G  TR N+   + I P   + +   +C A +  L     +  T ++SVLYPPG P I G   G  +  GQ+  L C S  GNP A L W++  E ++ + +         Y       TA+ +DN   Y+CEA+N        P  + +TLSV F P  + + S P   KAG K  L C T +SNP A++ W    G  + N   I   S P GG+++T+ + + V      +  IC   N  L E++    T+ V      +P+I    K   +I P  T+        ++  IS   NP      + N  ++ I +++  +D      S+   + +     ++   YK + +N   +      +K++V +    +    +   +  G+ A   C   +    A  +SW + G  + G     +  +  G  S      NV+ E  G  + C A N         +  L V  +P  +E      +A +     +         ++V++W++ G   S++     S  I D                  L+I  ++  D G Y C A N +G
Sbjct:   62 NNKAIRANARLTVIAPPSSIEII--NHSSKDKIQIKENEE------LEIECLVKNSKPPAKVIWYRGDIELKSDNSSDTVINVPE--GKVTRYNVLSKIKIKPTADDDVAEYTCEARHEALPTNMPLRSTVSLSVLYPPGLPYIEGYTEGETVKRGQTIELICRSRGGNPPAQLIWFKNGEPVRMSYRTAGRLSENVYT-----FTADSNDNKAKYKCEATN---VMSPIPYKAEVTLSVLFAPAHVTI-SGPTEAKAGDKVPLTCTTDNSNPPADIKWMV-AGRQVRNTSVITTPS-PNGGSISTSNITVTVGQNKRSLVVICHGLNMHLTENIVGTHTINVLYPPG-HPIISGYTKGT-NI-PVGTV-------QMISCISSGGNPLATLTWYKN--DKKITSVSKTNDR-----SVSAEITVYTNMTDNGAVYKCEASNPATEVPFIETVKLNVYFPPDHVRVRKEPAELKPGQIATLTCESSSSNPPAK-LSWWKEGIPVGGAINTTKPGLHGGVISTIELKVNVTPEINGVVYTCQATNAPLQRSVHDAFTLDVLFKPVFKELTNKIYSAVEGEPLLIFLETKANPSNIVYTWNKNGVSFSKK----NSRLIVDGPV---------------LNITRLTRHDNGEYKCTASNSQG 663          

HSP 3 Score: 78.1814 bits (191), Expect = 1.669e-13
Identity = 155/716 (21.65%), Postives = 273/716 (38.13%), Query Frame = 0
Query:    9 CVSHGGNPLAEVVWYRGDEQI--DFSYYTT-----GRESR-NMYTFVA---SSEDNNKRYRCKAKNTV--SVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETET-KNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFK---PITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIP-----NITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDITDAVM-VVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDR--MTWESELHIKNVSSRDYGSYDCIAQNEEG 694
            C+     P A+V+WYRGD ++  D S  T      G+ +R N+ + +    +++D+   Y C+A++    +  PL + + LSV + P    IEG TE      G T+   C +   NPPA + W  +         VR      G  + +  +     ND      C A N           T+SVL+ P   +I+G    T    G    LTC +   NP A + W      ++ T+     +    + + S + +T   +   +V  C   N    +    +  T T++V +    PI         +P  G    + C +   NP A + W +ND   I +  +   RS      + TN+        D+G  + CEA+N     +       TVK   +  P    + K   ++ P        E S         +NP      W   + E IP     N T    +   I +I+  +N+T   + +   Y  +  N+  +  +     +DVL+     ++T+ +   V GE         A P +    +W++ G     + S +   +   L +  +++ D GE+ CTA+N       T +  + V+    +  +P    A   +     L C   G P                 +ED+        Y++    I +  +   S L +++ + +D GS++C+  N  G
Sbjct:  104 CLVKNSKPPAKVIWYRGDIELKSDNSSDTVINVPEGKVTRYNVLSKIKIKPTADDDVAEYTCEARHEALPTNMPLRSTVSLSVLYPPGLPYIEGYTEGETVKRGQTIELICRSRGGNPPAQLIWFKNGEP------VRMSYRTAGRLSENVYTFTADSNDNKAKYKCEATNVMSPIPYKAEVTLSVLFAPAHVTISG---PTEAKAGDKVPLTCTTDNSNPPADIKWMVAGRQVRNTSVITTPSPNGGSISTSNITVTVGQNKRSLVVICHGLNMHLTEN---IVGTHTINVLYPPGHPIISGYTKGTNIP-VGTVQMISCISSGGNPLATLTWYKNDK-KITSVSKTNDRSVSAEITVYTNM-------TDNGAVYKCEASN----PATEVPFIETVKLNVYFPPDHVRVRKEPAELKPGQIATLTCESSS--------SNP-PAKLSW---WKEGIPVGGAINTTKPGLHGGVISTIELKVNVTP--EINGVVYTCQATNAPLQRSVHDAFTLDVLFKPVFKELTNKIYSAVEGEPLLIFLETKANP-SNIVYTWNKNGVSFSKKNS-RLIVDGPVLNITRLTRHDNGEYKCTASNSQGSASITFN--ISVQYSAYVVTAPQYVVATPGEN--VELNCTVDGNPL----------------KEDDISWRNKNYYDLDEKTISKTFINSTSYLVLRSPTRKDSGSFECVVNNGIG 758          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: EFX79012.1 (hypothetical protein DAPPUDRAFT_305049 [Daphnia pulex])

HSP 1 Score: 550.051 bits (1416), Expect = 2.498e-177
Identity = 332/854 (38.88%), Postives = 465/854 (54.45%), Query Frame = 0
Query:   81 APDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD--DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVR---------------------------QRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGM-VKPEDKDVE-RTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
             P  VT+ GP+EA V DT+S +C TTNSNP A + WV+D R  +EN +    S  GGW + SN++  I    RN  +SC+AI  DL + V +T  ISV+YPP  PSITG+   TI D     +L C+SM GNP ATL W+     + G+ ++   + T   + AQS L + A   DNG   RCEA+NPA     G L  + T+ + F P  + VK  PE  + G+   + CE+ SSNP + + W R +G  I+  G   V  G + G ++ + L ++V    HGV   C+ATN                            DKP F + PTD    V EG   +VN++ RANP +V+Y+W   + +      L   N   R+    +LLN+T  R++D G YK +  NSE              A+I      VMV  G  A  EC     P+T  T+SW R GFDMEGRT+   G    +LTV  +++ D G F C ANNGI + +  + T+L+V+                            +P +++SP   KAA D+ + A L CRAQG P++ F WSR G+ ++             +KY      ID +TWES L + NV SRDYG YDC+A+NE G +   V L+ TSRPD+P  L+V N T++S +L W  GFDGG  QS+ +RYR+ G++ Y+Y DV+ ++T +F +  L LGT+Y F + AFN+LG+S +T + V   T S       KP D +V    + + +I+ IT+V   LL+LNV LI+C+V+RR   R+  +  +EG    GS+KSATIEMYA SSYNET++GETLSSISEKS  Y+
Sbjct:   12 GPKTVTVTGPSEAKVNDTVSLECSTTNSNPVARITWVLDGREVRENGSRIATSPDGGWVSSSNLTYTIRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSITGMRDRTIRDN-TFYQLNCMSMGGNPPATLKWF-----LSGSRKEIKANYTSHGSVAQSLLEMQAKREDNGKELRCEATNPAL---EGNLVDSTTIRIEFAPKHLTVKVRPEKLRPGQTVTITCESDSSNPVSRLAWLRGNGQVIQPAGNSTV-PGAYSGIVSKSTLLMEVTPELHGVVITCQATN----------------------------DKPQF-LHPTDYSVDVIEGEPAIVNVTARANPSEVSYKW---FRDGTAIKQLKEANAYDRVTFDGSLLNLTTVRRDDKGEYKCEATNSEDP------------ASIVQTRSTVMVNEGSDAELECRAAGNPLTMATVSWRRAGFDMEGRTTQTLGVGVAYLTVKEITRNDTGAFECVANNGIGE-ESIEKTWLIVKCKFLPLLAPLPVVCLTRFSIGFLLGRADKPVMDDSPQLMKAAGDEGQLAKLVCRAQGAPNISFGWSREGTPLT-----------PSDKYSFSTRQIDIVTWESTLEVGNVRSRDYGLYDCVARNEMGTNTAKVILSGTSRPDSPLALHVVNVTHDSAHLIWRSGFDGGLFQSYRLRYRQIGNEFYKYADVYPTNTTAFTLGGLALGTEYLFGIMAFNNLGESNYTQDIVKARTSSETPPTGQKPRDLEVRPGDMPKVLIIVITIVGTALLVLNVALIACFVRRRHRKRLAKAMSSEG----GSSKSATIEMYAPSSYNETVNGETLSSISEKSESYS 795          

HSP 2 Score: 93.5893 bits (231), Expect = 2.912e-18
Identity = 126/548 (22.99%), Postives = 217/548 (39.60%), Query Frame = 0
Query:    7 LICVSHGGNPLAEVVWYRGD--EQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-GPTEANVGDTLSFDCITTNSNPPAIVQWVV-DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPL-ATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNP--TANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVE-------ARDHGVTFICEATNEELGESVNDGITMTVKCK--SFLNPL---------IDFI----DKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPN--ITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMV--VTGETAHF 521
            L C+S GGNP A + W+     ++I  +Y + G  ++++    A  EDN K  RC+A N      L     + ++FAP  +T++  P +   G T++  C + +SNP + + W+  + +  Q            G  ++S + + + P     +I+C A N+         +++ V+   G+P+I                   ++   NP   +  W+R    I+   ++ +  +   +  S L+LT    D+   Y+CEA+N                     P +I       +   G  A L C   + NP   A V WRR  G  +E +              TT  LG+ V         R+    F C A N  +GE   +   + VKCK    L PL         I F+    DKP+ D  P     + +EG    +    +  P ++++ W+       P+   +  +  I  I + ++ L +   R  D G Y     N  G +  K+   +L G +  D   A+ V  VT ++AH 
Sbjct:  134 LNCMSMGGNPPATLKWFLSGSRKEIKANYTSHGSVAQSLLEMQAKREDNGKELRCEATNPALEGNLVDSTTIRIEFAPKHLTVKVRPEKLRPGQTVTITCESDSSNPVSRLAWLRGNGQVIQPAGNSTVPGAYSGIVSKSTLLMEVTPELHGVVITCQATNDKPQFLHPTDYSVDVI--EGEPAIVN-----------------VTARANPSEVSYKWFRDGTAIK-QLKEANAYDRVTFDGSLLNLTTVRRDDKGEYKCEATNSE------------------DPASIVQTRSTVMVNEGSDAELECR-AAGNPLTMATVSWRR-AGFDMEGR--------------TTQTLGVGVAYLTVKEITRNDTGAFECVA-NNGIGEESIEKTWLIVKCKFLPLLAPLPVVCLTRFSIGFLLGRADKPVMDDSPQLMKAAGDEGQLAKLVCRAQGAP-NISFGWSREGTPLTPSDKYSFSTRQI-DIVTWESTLEVGNVRSRDYGLYDCVARNEMGTNTAKV---ILSGTSRPDSPLALHVVNVTHDSAHL 621          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: EAA07064.5 (AGAP010742-PA, partial [Anopheles gambiae str. PEST])

HSP 1 Score: 546.199 bits (1406), Expect = 5.534e-174
Identity = 357/999 (35.74%), Postives = 522/999 (52.25%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFE--VEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERT----------ISQGIILTITLVSGLLLILNV-ILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTSSSVDPIHGHHHHIHHHPHPLPHN--VPPSGGAXTGA-PKNNGGHNTHHHHTLPSSTSHNHHHHHQSNRRSHRREEESMIDKS 982
            GQ V LIC S GGNP A+++WY+ D Q+  +Y TT R S N+Y+FVA   DN  R RC+A N +   PL  +I LSV FAP +VTI G +EA  GD +S  C T  SNPPA ++W V     +   +   E+  GGW T SNISV +  N R+ ++ C+ +N  L E V+ THT++VL PP +P ITG   GTI+  G  +RL C S  GNPLATLTWY+ D+ +   T+  D++       SE+ +  N +DN   YRCEA N AT     P+S++ TL+V F P T+ +K  P   K G +A L C++ SSNP A + W R DG++++       + G +GG +++  L +++     G  + C++ NE L  +V++ I + V            +  P F   P+ T+    EG  L V +    NP  ++Y W     + +P   L S    RI S   +LNIT   + D G Y  +  NS+G + + I + V YGA+I  I++ V V  GE A   C V+ KP+T + I W R+G+DM  +TS    NGT+ +H  V N  +ED G F C A+N +A+   ++   L+V+  P I++SP   +AA+   + A L CRAQ  P   F WSR G V++           +  K+ +++  ID +T+ES L ++ V+S DYG Y+CIA+NE G  +  V L+VTS PD P  LNV N T++SV L W PGFDGG K ++ IRYR+  S  Y Y D   S  NS++  +  L++ T Y FS+ A N LG S             YL  + +   K  ER           I   +I  I L +  LL +N  ++I C +++R   +   S V        +     +  +   +    ++GETLSS+SEKS  Y++ +  P +            L  N  +PP      G  P      N  +  TLP    H  H+     +RSH   ++ MI++S
Sbjct:  236 GQNVELICRSRGGNPPAQLIWYKNDVQVRMAYRTTDRLSENIYSFVAEEGDNKARLRCEANNIMIAAPLKTEITLSVLFAPTQVTISGASEARTGDVVSLQCQTAPSNPPAEIKWSVSGHHVKNTTSRTIENPDGGWITLSNISVPVEANKRSLVVICHGLNMQLTENVIATHTVNVLLPPSQPVITGYTEGTIIAAGSVQRLQCTSSGGNPLATLTWYKNDKKLNTPTKTTDKS-----VSSEISILVNATDNQAKYRCEAHNSATEI---PMSASRTLAVNFPPETVKIKIIPPELKPGIEATLICDSSSSNPPATISWWR-DGIAVDGMNN-SSKPGLWGGTVSSLELKVNISQDMDGNVYTCQSANEALQRNVHEAIKLQV------------LYPPKFKPPPSSTVVGA-EGEPLTVAMVATGNPMSISYTWT---KDGLP--ILSSAGAQRIVSEGPILNITRLNRNDTGIYTCEAVNSQGSAMINISVVVEYGASIQSISENVTVNPGEEAMLSCTVEGKPLTEEHIRWERIGYDMTIKTSTTFANGTSYLH--VKNARREDVGNFRCIADNRVAN-PTSRDVLLIVKFAPEIDKSPVMLRAAAGFGERARLPCRAQAAPRPKFYWSRSGQVLNVN---------QSAKFYVEHKQIDALTYESILVVERVASNDYGLYECIARNELGNVKEKVRLDVTSPPDPPVSLNVLNVTHDSVTLAWTPGFDGGMKANYRIRYRELNSDRYWYED---SQPNSYKLTIGGLRMNTLYVFSIMASNGLGSSR------------YLPDVTRAHTKGKERQARLFMVQSAEIPSFVIFCIALAALCLLAVNAGLVIWCIIRKRAKGKQQSSRVP-------TLFFLLVPPFLVLNIRHFVTGETLSSVSEKSDAYSTDANRPDYTDDTTSKSASTYLVENGDMPPPRYQKEGTLPPYPNNINGAYARTLP----HPRHNPVNYQQRSH---DDQMIERS 1165          

HSP 2 Score: 127.487 bits (319), Expect = 1.021e-28
Identity = 145/554 (26.17%), Postives = 229/554 (41.34%), Query Frame = 0
Query:   48 ASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQ----ENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLG-ETVVQTHT-ISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGET-----AHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGT-NSMHLTVYNVSKE-DAGEFLCTANN 578
            AS ED+ + Y+C+         + A+  L+V   P  + I+G       E   G  L+  C+ +N+ P A + W       +    EN    TE +R  +   S + +    +D     +C A +  L  +  +QT   +SVLYPPG+P I G   G ++  GQ+  L C S  GNP A L WY+ D  ++   +  D      Y+       A   DN    RCEA+N   A    PL + ITLSV F P  + +    E  + G    L C+T  SNP A + W  + G  ++N    R    P GG +T + + + VEA    +  IC   N +L E+V    T+ V     L P      +P+       TI +      L    S   NP      + N    N P  T D        S+ + ++I     ++  +Y+ + +NS   + + +             T  + ++  E      A   C   +    A TISW R G  ++G     +  +  GT +S+ L V N+S++ D   + C + N
Sbjct:   77 ASIEDDAE-YQCQVGPAKFNSAIRANARLTVISPPSSIEIQGHSRNAKVEVREGQDLTLTCVVSNAKPVAQIVWYRGKTEYKSDTIENKVTETEGKR--YTVSSQLRIKPTSDDDYMEYTCQAKHKALQPDRPMQTKVQLSVLYPPGEPQIEGFTAGEVLRRGQNVELICRSRGGNPPAQLIWYKNDVQVRMAYRTTDRLSENIYS-----FVAEEGDNKARLRCEANNIMIA---APLKTEITLSVLFAPTQVTISGASEA-RTGDVVSLQCQTAPSNPPAEIKWSVS-GHHVKNTTS-RTIENPDGGWITLSNISVPVEANKRSLVVICHGLNMQLTENVIATHTVNV----LLPP-----SQPVITGYTEGTIIAAGSVQRLQCT-SSGGNPLATLTWYKNDKKLNTPTKTTDK-------SVSSEISILVNATDNQAKYRCEAHNS--ATEIPMSASRTLAVNFPPETVKIKIIPPELKPGIEATLICDSSSSNPPA-TISWWRDGIAVDGMNNSSKPGLWGGTVSSLELKV-NISQDMDGNVYTCQSAN 595          

HSP 3 Score: 88.9669 bits (219), Expect = 7.367e-17
Identity = 153/722 (21.19%), Postives = 273/722 (37.81%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGD------------EQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSV--EPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNY-AQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY--SIQNL-LNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDITDAVMV-VTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQN-GTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMT-WESELHIKNVSSRDYGSYDCIAQN 691
            GQ ++L CV     P+A++VWYRG              + +   YT   + R       +S+D+   Y C+AK+       P+   + LSV + P +  IEG T   V   G  +   C +   NPPA + W  +    Q     RT           NI   +     NK  + C A N  +   +    T+SVL+ P + +I+G    +    G    L C +   NP A + W      ++ TT    E     +   S + +    +   +V  C   N    Q +  + +T T++V   P    +    E  +  AG    L C +   NP A + W +ND   +    +   +S        ++ + I V A D+   + CEA N      ++   T+ V          +F  + +   I P +    +E  + L+ + S    P  +++     + + I    +++ + P ++  ++ +L L +  ++  D   Y  +  N   +  +   IK+ VLY         + +V   GE      V    P++  + +W++ G  +      Q   +    L +  +++ D G + C A N         S  ++V     I+    N     +  + A+L+C  +G P  +    W R+G                   Y++            S LH+KN    D G++ CIA N
Sbjct:  129 GQDLTLTCVVSNAKPVAQIVWYRGKTEYKSDTIENKVTETEGKRYTVSSQLR----IKPTSDDDYMEYTCQAKHKALQPDRPMQTKVQLSVLYPPGEPQIEGFTAGEVLRRGQNVELICRSRGGNPPAQLIWYKN--DVQVRMAYRTTD-----RLSENIYSFVAEEGDNKARLRCEANNIMIAAPLKTEITLSVLFAPTQVTISG---ASEARTGDVVSLQCQTAPSNPPAEIKWSVSGHHVKNTTSRTIENPDGGWITLSNISVPVEANKRSLVVICHGLN---MQLTENVIATHTVNVLLPPSQPVITGYTEGTIIAAGSVQRLQCTSSGGNPLATLTWYKNDK-KLNTPTKTTDKS-------VSSEISILVNATDNQAKYRCEAHNSATEIPMSASRTLAV----------NFPPETVKIKIIPPELKPGIE--ATLICDSSSSNPPATISW-----WRDGIAVDGMNNSSKPGLWGGTVSSLELKVNISQDMDGNVYTCQSANEALQRNVHEAIKLQVLYPPKFKPPPSSTVVGAEGEPLTVAMVATGNPMSI-SYTWTKDGLPILSSAGAQRIVSEGPILNITRLNRNDTGIYTCEAVNSQGSAMINIS--VVVEYGASIQSISEN--VTVNPGEEAMLSCTVEGKPLTEEHIRWERIG-------------------YDMTIKTSTTFANGTSYLHVKNARREDVGNFRCIADN 784          
BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Match: XP_006564422.1 (PREDICTED: nephrin isoform X5 [Apis mellifera])

HSP 1 Score: 497.278 bits (1279), Expect = 6.417e-156
Identity = 323/907 (35.61%), Postives = 474/907 (52.26%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIF-DIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDME--GR--TSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKG--SKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSG--LLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYN-ETMSGETLSSISEK 897
            GQ + L C S GGNP  ++ WY+  E+I   Y   G  S N+ +  A ++DNN RYRC+  N +SVEPL   + L+V FAP  VTI GPTEA   + +   C T NSNPPA ++W VD  + + N +    +  GGW T SN++  I    R+ ++ C A N  L E VV+THTI+V+YPP K SITG + GT +D G   RL C + +GNPLATL+WY+ D  + GTT+  D         SEL +  N SDN    RCEA+N AT     PL   + L V F P  + +  +P+   AG++  + CE+ SSNP A + W +  G+ ++       + G  GG L+   L +DV    +G  + C+A N ++  S++D  T+            D + KPIF  I+P + I    E    V+++S   NP +++Y W     + +P ++    N  RI    + LNIT+  + DAG Y  +  N EG +  ++ + V Y A I    T  ++   G  A   C VD  PI  + ++W +VG + E  GR  TS  N T+ +H+   N  +ED GE+ C  NNGI ++  ++    +   +P++  +P   +AA++K     L C+A+G+P   FSW+  G  +           + + KY I +  +  +   S L I  V+S DYG Y+C A N+ G S   ++L+VTS PD P+ L VFN T++SV L W  GFDGG   S+ IR+R+       Y Y+DV      +  +  L LGT Y FSVKA N+ GDS F  + V V+TL        P D       S  II+ I  VS    LLI   I+ +    +++  R            +G  KS T +MYA S+ N +TM+GE LSS+S++
Sbjct:  276 GQQLDLTCRSRGGNPPPQIFWYKNSERISSPYRNEGHFSENVLSITAKAQDNNARYRCEVFNIMSVEPLKVHVDLTVLFAPSGVTITGPTEAKADEQVVITCTTENSNPPADIKWTVDGHNFESNASRTEPAPEGGWITTSNVTFNINRTSRSIVVICDASNVKLTENVVRTHTITVIYPPSKLSITGYEEGTTIDAGTVLRLLCTATSGNPLATLSWYKNDRKVPGTTRTRDHA-----VSSELTILINASDNNARIRCEAANSATQI---PLLKVLVLKVNFPPDKVKITREPQNLHAGQEGRIICESSSSNPAAEMSWWKG-GIPVQGTKN-GTKPGLHGGFLSFVELSLDVTEEMNGEVYTCQARNNQMDRSIHDATTL------------DVLYKPIFSSIEPYELIGM--ESEPFVISVSAMGNPNEISYTWT---RDGLPLVS----NNRRISVRASTLNITKLDRHDAGTYICEATNEEGTTFYQLNLTVQYSAKIKRTSTSGIVYSPGIEAKLFCEVDGSPIGDEYVTWQKVGSNSELSGRYSTSFINKTSYLHIE--NPDQEDVGEYQCKVNNGIGNVT-SEPILFITNFKPQMMNTPLTRRAAANKGANVQLLCKARGSPLPRFSWTFNGKTL--------LPNVTEHKYGITHTDLSELISNSTLTIFRVTSHDYGKYECRATNKMGQSTDMIHLDVTSPPDKPSDLEVFNVTHDSVTLVWKRGFDGGLPTSYQIRWRQALDYEHRYHYLDVSAGEYKT-TITGLSLGTYYVFSVKAINEKGDSGFLPDLVKVQTLR----EAPPTDVTSSEINSSYIIVIIITVSASVCLLIAAPIVFATLKSKKKAQR------------SGINKSQTADMYAPSTVNGDTMTGE-LSSVSDE 1122          

HSP 2 Score: 133.65 bits (335), Expect = 1.454e-30
Identity = 167/716 (23.32%), Postives = 300/716 (41.90%), Query Frame = 0
Query:   37 GRESRNMYTF--VASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRT-----ESERGG--------WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD--TIFSVEEGSDLVVNISVR-------ANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV-------TGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            G +S  +Y      +S  ++  Y+C+    + ++ + A+  L+V   P KV I     +   E  VG++   +C+  ++ P A + W    +  +  + + T  +     E ++ G        ++T  +I ++   +D +   +C A +  L  ++    TI  SV YPP  P I G   G  +  GQ   LTC S  GNP   + WY+  E I    +++      +++++ L +TA   DN   YRCE  N  + +   PL   + L+V F P  + + + P   KA ++  + C T +SNP A++ W   DG + E+    R    P GG +TT+ +  ++      +  IC+A+N +L E+V    T+TV                   I P    +I   EEG+ +     +R        NP      + N  +  +P  T   D     +++ + L I     ++  R + +  NS  +  L +K+ VL    ++   D V +         G+     C   +    A+ +SW + G  ++G     +  +  G  S      +V++E  GE + C A N   D     +T L V  +P I  S    +    + +  V++  A G P ++ ++W+R G  +             + +  ++          S L+I  +   D G+Y C A NEEG + Y + L V
Sbjct:   89 GDQSTGVYNLQITQASLTDDGEYQCQVGPYLRIKSIRANAHLTVISPPQKVEISNHPNKKKIEVKVGESRRLECVVRSAKPAATITWYRGNIQIKGGETSITPISIEGDKEVQKPGENKKELLKYDTHGSIVIVPTADDNDMDYTCEANHPALPYSMPLRATIKLSVFYPPTMPYIEGYTEGETVKRGQQLDLTCRSRGGNPPPQIFWYKNSERISSPYRNEG-----HFSENVLSITAKAQDNNARYRCEVFNIMSVE---PLKVHVDLTVLFAPSGVTI-TGPTEAKADEQVVITCTTENSNPPADIKWTV-DGHNFESNAS-RTEPAPEGGWITTSNVTFNINRTSRSIVVICDASNVKLTENVVRTHTITV-------------------IYPPSKLSITGYEEGTTIDAGTVLRLLCTATSGNPLATLSWYKN--DRKVPGTTRTRD-----HAVSSELTILINASDNNARIRCEAANSATQIPL-LKVLVL---KVNFPPDKVKITREPQNLHAGQEGRIICESSSSNPAAE-MSWWKGGIPVQGTKNGTKPGLHGGFLSFVELSLDVTEEMNGEVYTCQARNNQMDRSIHDATTLDVLYKP-IFSSIEPYELIGMESEPFVISVSAMGNPNEISYTWTRDGLPLVS----------NNRRISVR---------ASTLNITKLDRHDAGTYICEATNEEGTTFYQLNLTV 742          

HSP 3 Score: 102.449 bits (254), Expect = 4.570e-21
Identity = 173/753 (22.97%), Postives = 285/753 (37.85%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQI--------------DFSYYTTGRESRNMYTF--------VASSEDNNKRYRCKAKNTV--SVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE--VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYS-----IQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITAD-TISWSRVGFDMEG---RTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTF----LLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            +G++  L CV     P A + WYRG+ QI              D      G   + +  +        V +++DN+  Y C+A +       PL A I LSV + P    IEG TE      G  L   C +   NPP  + W  +S      +  R E    G  + + +S+     D N    C   N    E +     ++VL+ P   +ITG    T     +   +TC +   NP A + W   G       ++ +   E      S +    N +   +V  C+ASN    +    +  T T++V + P  +++    E     AG    L C   S NP A + W +ND    +  G  R R       LT     I + A D+     CEA N      +   + + V             DK     +P + + + +EG   ++  S  +NP      W       IP     +   P ++      ++  L++TE    +    + + N  +        +DVLY    S I +   ++  E+  F   V A     + + +W+R G  +     R S++  T    L +  + + DAG ++C A N      +  +TF    L V+   +I+ +  +    S   + A L C   G+P  D   +W ++GS          + E+   +Y   +  I++ ++   LHI+N    D G Y C   N  G        NVTS P
Sbjct:  154 VGESRRLECVVRSAKPAATITWYRGNIQIKGGETSITPISIEGDKEVQKPGENKKELLKYDTHGSIVIVPTADDNDMDYTCEANHPALPYSMPLRATIKLSVFYPPTMPYIEGYTEGETVKRGQQLDLTCRSRGGNPPPQIFWYKNSERISSPY--RNE----GHFSENVLSITAKAQDNNARYRCEVFNIMSVEPLKVHVDLTVLFAPSGVTITG---PTEAKADEQVVITCTTENSNPPADIKWTVDGHNFESNASRTEPAPEGGWITTSNVTFNINRTSRSIVVICDASNVKLTEN---VVRTHTITVIYPPSKLSITGYEEGTTIDAGTVLRLLCTATSGNPLATLSWYKNDR---KVPGTTRTRDHAVSSELT-----ILINASDNNARIRCEAANSATQIPLLKVLVLKVNFPP---------DKVKITREPQN-LHAGQEGR--IICESSSSNPAAEMSWWKG----GIPVQGTKNGTKPGLHGGFLSFVELSLDVTEEMNGEVYTCQARNNQMDRSIHDATTLDVLYKPIFSSI-EPYELIGMESEPFVISVSAMGNPNEISYTWTRDGLPLVSNNRRISVRAST----LNITKLDRHDAGTYICEATN------EEGTTFYQLNLTVQYSAKIKRTSTSGIVYSPGIE-AKLFCEVDGSPIGDEYVTWQKVGS----------NSELSG-RYSTSF--INKTSY---LHIENPDQEDVGEYQCKVNNGIG--------NVTSEP 834          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|241839651|ref|XP_002415268.1| (nephrin, putative [Ixodes scapularis] >gi|215509480|gb|EEC18933.1| nephrin, putative, partial [Ixodes scapularis])

HSP 1 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 388/909 (42.68%), Postives = 538/909 (59.19%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQ--IDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRN-KIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNE-NIPNITLDSDN--IPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGS-KTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MG T +L C+S GGNPLA++VWYR  E+  +D SY T+GRES N  TFVA+S DNN  YRC A N VS EP++A + L+VQFAP KV + GP EA  GD ++  C T  SNPPA V WVVD R  +   T+ T   +GGW T SNI+V I   +RN K+ SCYA+N  LG+TVV+T  +S+LY P  P I G   GT +     + +TC++  GNPL T+ W++G + +  TT+ +      N   +E+ + A  SDNG VYRCEASNPAT +    L+++I L+V F P  + +K  P  PK+G+K  L CETGSS+P +++ W + DGL +    +  V  GPFGG  + NVL I+V A D    ++C+ATN  L  SV+D +T+            D   KP F   P +  F + EG  LV+N++ RANP  VTY W            T  S +  +P +Y+  +LLN+T   +   G Y  +  NSEG ++  I I++ YGA +  +T+ V+V  G  A  EC VDAKP   DT+SW R GFDM  RT         +LTV+NVS++D+G F C A+NGI + +  K+  L+V+ RP I+ SP + KAA  +  TA L CRAQ  P+  F+WSR GSVI+     D        KY++    +D +T+ES L +K + S DYG Y+C+A+NE G     V+L+  S PD P  L V N TYNS+ L W PGFDGG  QSF +RYRK  S + Y Y DV  S+  ++ +  L   T+Y+  + AFN+ G  E+  + V  +T       V+P       T+   II ++++V   LL LN++L+ C+V++RR  R+      E   ++ S+K+ATIEMYA SSYNETM+GET+SS SEKS  Y+
Sbjct:  185 MGDTTTLRCISRGGNPLAQLVWYRNQEERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPEPVSAAVRLTVQFAPSKVKVRGPKEAKAGDQITMRCSTERSNPPAEVSWVVDGRPFESVSTI-TADPKGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDTVVETAAVSILYAPDAPRIFGYTEGTPIRAKTLQHITCVTNGGNPLPTVRWFKGTQKVNSTTKTNG-----NSVSAEVAIIAQDSDNGAVYRCEASNPATKKA---LTTSIKLTVHFPPTAVTIKIKPRKPKSGQKMTLTCETGSSHPQSSISWWK-DGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTL------------DVTYKPEFYNAPLER-FDIVEGESLVINLTARANPSAVTYSWTKDERRVATARATERSADRALPSVYARGSLLNLTNVERSHGGVYDCEAENSEGSTKTSILINIQYGAIVKAVTETVIVDAGSHAQLECEVDAKPFDEDTVSWRRQGFDM-SRTRQLVKDRRAYLTVHNVSRQDSGPFDCVAHNGIGE-ESVKTANLVVKFRPEIDRSPPHLKAAGQEGGTAELLCRAQAAPNATFAWSREGSVITSSSRPD--------KYDVSVAQVDLVTFESTLSVKAIRSSDYGHYECVARNELGFDSAKVHLDTLSAPDPPLQLQVTNATYNSLTLVWRPGFDGGLPQSFRLRYRKAASNEGYHYQDVLLSNMTNYTIGGLGHDTEYTVGIMAFNEYGQGEYLKDIVKGKTTGECLPTVRPMGSLAVPTV---IITSVSVVGTSLLFLNIVLVICFVRKRRKKRL------EEEESDQSSKTATIEMYAPSSYNETMNGETVSSTSEKSEAYS 1051          

HSP 2 Score: 158.688 bits (400), Expect = 1.027e-35
Identity = 175/691 (25.33%), Postives = 296/691 (42.84%), Query Frame = 0
Query:   52 DNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIE-----GPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGV-TFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-------VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI--------TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQ------NGTNSMHLTVYNVSK-EDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAV-LTCRAQGTPDVVFSWS----RMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            D++  Y+C+   T   + + AD  ++V   P  VTI         E    +++   C+ + + P A ++W    V+ R    +++V  ESE G     S++++    +D   + SC A++  L   +  +  + VL+ PG P I G   G  +  G +  L CIS  GNPLA L WYR  E     + D   T +   + + L   AN SDN  VYRC A+N  + +   P+S+ + L+V+F P  + V+  P+  KAG +  + C T  SNP A V W   DG   E+   I   + P GG +TT+ + +++  ++  +  F C A N+ LG++V +   +++       P     D P         IF   EG+ +       +  ++   NP   T RW     + + + T  + N     S+   + I     ++   Y+ + +N   K  L   IK+ V +  T   I        +   M +T ET        + P    +ISW + G  + G            G  S ++ V NV+  +D   ++C A N    +    S  L V  +P    +P       +     + LT RA  +  V +SW+    R+ +  + E   D +            +V  R    S L++ NV     G YDC A+N EG ++ ++ +N+
Sbjct:   32 DDDAEYQCQVGPTSDHKAIRADAHVTVLLPPASVTITDHPNGSSVEVRQSESVKLTCVVSQAKPAAQLKWYRKNVELRPDNVHYSVE-ESEGGRQNAISSLTLTPHSDDNGVVYSCEAVHPALESPMATSVRLGVLFAPGPPEIHGYTEGETIRMGDTTTLRCISRGGNPLAQLVWYRNQE---ERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPE---PVSAAVRLTVQFAPSKVKVRG-PKEAKAGDQITMRCSTERSNPPAEVSWVV-DGRPFESVSTI--TADPKGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDTVVETAAVSI----LYAP-----DAP--------RIFGYTEGTPIRAKTLQHITCVTNGGNPLP-TVRWFKG-TQKVNSTTKTNGN-----SVSAEVAIIAQDSDNGAVYRCEASNPATKKALTTSIKLTVHFPPTAVTIKIKPRKPKSGQKMTLTCETG------SSHP--QSSISWWKDGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTLDVTYKPEFYNAPLERFDIVEGESLVINLTARANPSA-VTYSWTKDERRVATARATERSADRALP----------SVYAR---GSLLNLTNVERSHGGVYDCEAENSEGSTKTSILINI 665          

HSP 3 Score: 104.375 bits (259), Expect = 5.806e-19
Identity = 174/744 (23.39%), Postives = 282/744 (37.90%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQI--DFSYYTT-----GRESR-NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY---RGDEIIQGTTQDDDETETKNYAQSELHLTANYSD---NGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE-VP-KAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWS----RVGFDMEGRTSMQNGTNSMH-----LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVY---LNVTSRPD 709
            ++V L CV     P A++ WYR + ++  D  +Y+      GR++  +  T    S+DN   Y C+A +     P+   + L V FAP    I G TE     +GDT +  CI+   NP A + W  +    QE  +V +     G E+ + ++ +   +D N +  C A N    E V     ++V + P K  + G       D+   R   C +   NP A ++W    R  E +   T D      K    +  ++T N +    N  ++ C A N A       +  T  +S+ + P    +    E  P +A    ++ C T   NP   V W +         G  +V S     GN  +  + I  +  D+G  + CEA+N    +++   I +TV    F    +    KP           + E GS         ++P      W +          +  D      + +N+L I     +D   Y  +  N   +  +   + +DV Y     +   +   +V GE+        A P +A T SW+    RV        S      S++     L + NV +   G + C A N       TK++ L+  Q   I ++        D    A L C     P  +   SW R G  +S+                 +  V DR  +   L + NVS +D G +DC+A N  GI   +V    L V  RP+
Sbjct:   82 ESVKLTCVVSQAKPAAQLKWYRKNVELRPDNVHYSVEESEGGRQNAISSLTLTPHSDDNGVVYSCEAVHPALESPMATSVRLGVLFAPGPPEIHGYTEGETIRMGDTTTLRCISRGGNPLAQLVWYRN----QEERSVDSSYTTSGRESVNTLTFVANSSDNNAVYRCVATNVVSPEPVSAAVRLTVQFAPSKVKVRGPKEAKAGDQITMR---CSTERSNPPAEVSWVVDGRPFESVSTITADP-----KGGWVTTSNITVNITGQERNMKMFSCYAVNQALGDT---VVETAAVSILYAPDAPRIFGYTEGTPIRAKTLQHITCVTNGGNPLPTVRWFK---------GTQKVNSTTKTNGNSVSAEVAIIAQDSDNGAVYRCEASNPATKKALTTSIKLTVH---FPPTAVTIKIKPRKPKSGQKMTLTCETGS---------SHPQSSISWWKDGLQLTGGVDDVVVDGPFGGRASKNVLVINVTADDDRANYMCQATNRALQLSVHDSVTLDVTYKPEFYNAPLERFDIVEGESLVINLTARANP-SAVTYSWTKDERRVATARATERSADRALPSVYARGSLLNLTNVERSHGGVYDCEAENSEG---STKTSILINIQYGAIVKA-VTETVIVDAGSHAQLECEVDAKPFDEDTVSWRRQGFDMSR----------------TRQLVKDRRAY---LTVHNVSRQDSGPFDCVAHN--GIGEESVKTANLVVKFRPE 763          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|942305741|gb|JAN34803.1| (Down syndrome cell adhesion molecule [Daphnia magna])

HSP 1 Score: 685.641 bits (1768), Expect = 0.000e+0
Identity = 378/907 (41.68%), Postives = 536/907 (59.10%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD--DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYL-EGMVKPEDKDVE-RTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MGQ V L C+S GGNPLA++VWY+ + +ID +Y TTGRESRN++ F A + DNN R+RC+A N ++  P+ A+I L+V F P  VT+ GP+EA V DT+S +C TTNSNP A + WV+D R  +EN +    S  GGW + SN++  +    RN  +SC+AI  DL + V +T  ISV+YPP  PSI+G+   TI D     ++ C+SM GNP ATL W+     + G+ ++   + T   + AQS L + A   DNG   RCEASNPA     G L  + T+ + F P  + VK  PE  + G+   + CE+ SSNP + + W R +G  I+  G   V  G + G ++ + L ++V    HGV   C+ATN  +G  ++D IT+ V            + KP F   P+D    V EG   +VN++ RANP ++TY+W   + +      L   N   RI     LLN+T  R++D G YK    N+EG     ++++V Y A+I      VM+  G  A FEC     P+T  T+ W R GFDMEGRTS   G    +L V  +++ D G F C ANNGI   +  + T+L+V+ +P +++SP  +KAA D+ + A L CRAQG P++ F WSR G+ ++             +KY      +D +TWES + + NV SRDYG YDCIA+NE G +   V L+ TSRPD P  L+V N T++SVYL W  GFDGG  QS+ +RYR+ G++ Y+Y DV+ ++  +F +  L LGT+Y F + AFN+LG+S +T + V   T S       KP D +V    + + +I+ IT+V   LL+LNV LI+C+V+RR   R+  +  +EG    GS+KSATIEMYA SSYNET++GETLSSISEKS  Y+
Sbjct:  258 MGQEVKLECISRGGNPLAQIVWYKNEVKIDHAYETTGRESRNVHIFRAEASDNNARFRCEASNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVNDTVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRDN-SFYQMNCMSMGGNPPATLKWF-----LSGSRKEIKANYTNHGSVAQSLLEMQAKREDNGKELRCEASNPAL---EGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVSRLTWLRGNGQVIQPAGNSTV-PGAYSGIVSKSTLQMEVTPELHGVVITCQATN-GIGPQIHDAITLEV------------LYKPQFQ-QPSDYSVDVIEGEPAIVNLTARANPSEMTYKW---FRDGTAIKQLKEANAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNVQYPASIVQARSTVMINEGSDAEFECRAAGNPLTTTTVFWRRAGFDMEGRTSQTPGVGVAYLLVREITRNDTGAFECVANNGIGQ-ESIEQTWLVVKYKPLMDDSPQLTKAAGDEGQLAKLVCRAQGAPNISFGWSREGTPLN-----------PSDKYSFSTRQVDIVTWESTMGVSNVRSRDYGLYDCIARNEMGTNSAKVVLSGTSRPDPPLALHVVNVTHDSVYLAWRSGFDGGLFQSYRLRYRQIGNEFYKYADVYPTNVTAFTLGGLALGTEYLFGIMAFNNLGESNYTQDIVKARTSSETPPSGQKPRDLEVRPGDMPKVLIIVITIVGTALLVLNVALIACFVRRRHRKRLAKAMSSEG----GSSKSATIEMYAPSSYNETVNGETLSSISEKSESYS 1121          

HSP 2 Score: 150.599 bits (379), Expect = 4.246e-33
Identity = 173/676 (25.59%), Postives = 286/676 (42.31%), Query Frame = 0
Query:   52 DNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQ-ENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAV---MVVTGETAHFECVVDAK-PITADTISWSRVGFDM---EGRTSMQ---NGTNSMHLTVYNVSKEDAGEFL-CTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESE-LHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            D++  Y+C+       +P+ AD  L+V   P  V I          +  G+ L   C+  N+ P A + W    V+ +S + EN T   E  R  ++TRS +++   P+D      C A +  L      +  +++ YPPG P I G   G  +  GQ  +L CIS  GNPLA + WY+ +  I      D   ET       +H+  A  SDN   +RCEASN  T   + P+ + ITL+V F P T+ V + P   K      L C T +SNP A + W   DG  +   G  R+ + P GG ++++ L   V A    VT  C A   +L + V +   ++V        +    D+ I D    ++ + +         +S+  NP   T +W    + +   I  +  N   +   Q+LL +   R+++    + + +N   +  L     +        +T  V    +  G+     C  D+  P++   ++W R    +    G +++    +G  S       V+ E  G  + C A NGI        T L V  +P+ ++ P++      + + A++   A+  P ++ + W R G+ I Q  E                N  DR+T++   L++  V   D G Y C A N EG   + V LNV
Sbjct:  104 DDDAEYQCQVGPRGLSKPIRADAQLTVLIPPSSVEIVDYVAGSRIMSREGEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRR-FDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKI------DHAYETTGRESRNVHIFRAEASDNNARFRCEASNALT---TTPMKAEITLTVHFGPKTVTV-TGPSEAKVNDTVSLECSTTNSNPAARITWVL-DGREVRENGS-RIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRD----NSFYQMN-------CMSMGGNP-PATLKWF--LSGSRKEIKANYTNHGSV--AQSLLEMQAKREDNGKELRCEASNPALEGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVS--RLTWLRGNGQVIQPAGNSTVPGAYSGIVSKSTLQMEVTPELHGVVITCQATNGIGPQIHDAIT-LEVLYKPQFQQ-PSDYSVDVIEGEPAIVNLTARANPSEMTYKWFRDGTAIKQLKEA---------------NAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNV 731          

HSP 3 Score: 81.6481 bits (200), Expect = 6.518e-12
Identity = 91/401 (22.69%), Postives = 168/401 (41.90%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID---FSYYTTGRE------SRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTH-TISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAY-LFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVK 387
            G+ + L C+     P AE+VW++ + +I        T+  E      +R+  T  A  +DN   Y C+A++   + P  A + L++Q+ P    I G TE     +G  +  +CI+   NP A + W  +       +      E  G E+R+        +D N    C A +N L  T ++   T++V + P   ++TG     + D   +  L C +   NP A +TW   G E+ +  ++     +    + S L  T   +   +   C A     AQ    ++ T  +SV + P   ++    +        Y + C +   NP A + W  +      ++ EI+       G++  ++L +  +  D+G    CEA+N  L  ++ D  T+ ++
Sbjct:  153 GEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRRFDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKIDHAY------ETTGRESRNVHIFRAEASDNNARFRCEA-SNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVND---TVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQR---VAETKIISVVYPPEAPSISGMRDRTIRDNSFYQMNCMSMGGNPPATLKWFLSG-----SRKEIKANYTNH-GSVAQSLLEMQAKREDNGKELRCEASNPALEGNLVDSTTIRIE 534          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|942394527|gb|JAN79193.1| (Down syndrome cell adhesion molecule [Daphnia magna])

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 378/938 (40.30%), Postives = 536/938 (57.14%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGP-------------------------------NDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD--DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYL-EGMVKPEDKDVE-RTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MGQ V L C+S GGNPLA++VWY+ + +ID +Y TTGRESRN++ F A + DNN R+RC+A N ++  P+ A+I L+V F P  VT+ GP+EA V DT+S +C TTNSNP A + WV+D R  +EN +    S  GGW + SN++  +                                   RN  +SC+AI  DL + V +T  ISV+YPP  PSI+G+   TI D     ++ C+SM GNP ATL W+     + G+ ++   + T   + AQS L + A   DNG   RCEASNPA     G L  + T+ + F P  + VK  PE  + G+   + CE+ SSNP + + W R +G  I+  G   V  G + G ++ + L ++V    HGV   C+ATN  +G  ++D IT+ V            + KP F   P+D    V EG   +VN++ RANP ++TY+W   + +      L   N   RI     LLN+T  R++D G YK    N+EG     ++++V Y A+I      VM+  G  A FEC     P+T  T+ W R GFDMEGRTS   G    +L V  +++ D G F C ANNGI   +  + T+L+V+ +P +++SP  +KAA D+ + A L CRAQG P++ F WSR G+ ++             +KY      +D +TWES + + NV SRDYG YDCIA+NE G +   V L+ TSRPD P  L+V N T++SVYL W  GFDGG  QS+ +RYR+ G++ Y+Y DV+ ++  +F +  L LGT+Y F + AFN+LG+S +T + V   T S       KP D +V    + + +I+ IT+V   LL+LNV LI+C+V+RR   R+  +  +EG    GS+KSATIEMYA SSYNET++GETLSSISEKS  Y+
Sbjct:  258 MGQEVKLECISRGGNPLAQIVWYKNEVKIDHAYETTGRESRNVHIFRAEASDNNARFRCEASNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVNDTVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRAXXXXXXXXXXXXXXXXXXXXXXXXXXXPVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRDN-SFYQMNCMSMGGNPPATLKWF-----LSGSRKEIKANYTNHGSVAQSLLEMQAKREDNGKELRCEASNPAL---EGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVSRLTWLRGNGQVIQPAGNSTV-PGAYSGIVSKSTLQMEVTPELHGVVITCQATNG-IGPQIHDAITLEV------------LYKPQFQ-QPSDYSVDVIEGEPAIVNLTARANPSEMTYKW---FRDGTAIKQLKEANAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNVQYPASIVQARSTVMINEGSDAEFECRAAGNPLTTTTVFWRRAGFDMEGRTSQTPGVGVAYLLVREITRNDTGAFECVANNGIGQ-ESIEQTWLVVKYKPLMDDSPQLTKAAGDEGQLAKLVCRAQGAPNISFGWSREGTPLN-----------PSDKYSFSTRQVDIVTWESTMGVSNVRSRDYGLYDCIARNEMGTNSAKVVLSGTSRPDPPLALHVVNVTHDSVYLAWRSGFDGGLFQSYRLRYRQIGNEFYKYADVYPTNVTAFTLGGLALGTEYLFGIMAFNNLGESNYTQDIVKARTSSETPPSGQKPRDLEVRPGDMPKVLIIVITIVGTALLVLNVALIACFVRRRHRKRLAKAMSSEG----GSSKSATIEMYAPSSYNETVNGETLSSISEKSESYS 1152          

HSP 2 Score: 129.028 bits (323), Expect = 2.042e-26
Identity = 101/315 (32.06%), Postives = 146/315 (46.35%), Query Frame = 0
Query:   52 DNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQ-ENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGG-----NLTTNV 351
            D++  Y+C+       +P+ AD  L+V   P  V I          +  G+ L   C+  N+ P A + W    V+ +S + EN T   E  R  ++TRS +++   P+D      C A +  L      +  +++ YPPG P I G   G  +  GQ  +L CIS  GNPLA + WY+ +  I      D   ET       +H+  A  SDN   +RCEASN  T   + P+ + ITL+V F P T+ V + P   K      L C T +SNP A + W   DG  +   G  R+ + P GG     NLTT V
Sbjct:  104 DDDAEYQCQVGPRGLSKPIRADAQLTVLIPPSSVEIVDYVAGSRIMSREGEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRR-FDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKI------DHAYETTGRESRNVHIFRAEASDNNARFRCEASNALT---TTPMKAEITLTVHFGPKTVTV-TGPSEAKVNDTVSLECSTTNSNPAARITWVL-DGREVRENGS-RIATSPDGGWVSSSNLTTTV 405          

HSP 3 Score: 71.2478 bits (173), Expect = 1.124e-8
Identity = 70/269 (26.02%), Postives = 117/269 (43.49%), Query Frame = 0
Query:  127 FTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGT-IMD-EGQSRRLTCISMAGNPLATLTWYRGD-----EIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKP--ITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
            +T+    E+G      NI +     D +    C      L + +     ++VL PP    I     G+ IM  EG+   L C+     P A + W++ +     E I+ TT  D E   +   +S+L + A   DN   Y CEA +PA      P  +++ L++++ P    I   ++ E  + G++  L C +   NP A ++W +N+ + I++  E         G  + NV     EA D+   F CEA+N      +   IT+TV
Sbjct:   83 YTIMGSEEQG----EHNIRIENATLDDDAEYQCQVGPRGLSKPIRADAQLTVLIPPSSVEIVDYVAGSRIMSREGEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTT-SDAELPRRFDTRSKLTIRAQPDDNEADYICEARHPALI---APKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNE-VKIDHAYETT-------GRESRNVHIFRAEASDNNARFRCEASNALTTTPMKAEITLTV 335          

HSP 4 Score: 70.8626 bits (172), Expect = 1.355e-8
Identity = 59/231 (25.54%), Postives = 101/231 (43.72%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID---FSYYTTGRE------SRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTH-TISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW 219
            G+ + L C+     P AE+VW++ + +I        T+  E      +R+  T  A  +DN   Y C+A++   + P  A + L++Q+ P    I G TE     +G  +  +CI+   NP A + W  +       +      E  G E+R+        +D N    C A +N L  T ++   T++V + P   ++TG     + D   +  L C +   NP A +TW
Sbjct:  153 GEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRRFDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKIDHAY------ETTGRESRNVHIFRAEASDNNARFRCEA-SNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVND---TVSLECSTTNSNPAARITW 373          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|942305739|gb|JAN34802.1| (Down syndrome cell adhesion molecule [Daphnia magna])

HSP 1 Score: 659.062 bits (1699), Expect = 0.000e+0
Identity = 373/910 (40.99%), Postives = 534/910 (58.68%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD--DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQG-----IILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MGQ V L C+S GGNPLA++VWY+ + +ID +Y TTGRESRN++ F A + DNN R+RC+A N ++  P+ A+I L+V F P  VT+ GP+EA V DT+S +C TTNSNP A + WV+D R  +EN +    S  GGW + SN++  +    RN  +SC+AI  DL + V +T  ISV+YPP  PSI+G+   TI D     ++ C+SM GNP ATL W+     + G+ ++   + T   + AQS L + A   DNG   RCEASNPA     G L  + T+ + F P  + VK  PE  + G+   + CE+ SSNP + + W R +G  I+  G   V  G + G ++ + L ++V    HGV   C+ATN  +G  ++D IT+ V            + KP F   P+D    V EG   +VN++ RANP ++TY+W   + +      L   N   RI     LLN+T  R++D G YK    N+EG     ++++V Y A+I      VM+  G  A FEC     P+T  T+ W R GFDMEGRTS   G    +L V  +++ D G F C ANNGI   +  + T+L+V+ +P +++SP  +KAA D+ + A L CRAQG P++ F WSR G+ ++             +KY      +D +TWES + + NV SRDYG YDCIA+NE G +   V L+ TSRPD P  L+V N T++SVYL W  GFDGG  QS+ +RYR+ G++ Y+Y DV+ ++  +F +  L LGT+Y F + AFN+LG+S +T + V   T S        +D + +  +++G     II+ ++    LLL+LNV L+ CY+ RRR  +    +++  G   GS+KSATIEMYA SSYNET++GETLSSISEKS  Y+
Sbjct:  258 MGQEVKLECISRGGNPLAQIVWYKNEVKIDHAYETTGRESRNVHIFRAEASDNNARFRCEASNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVNDTVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRDN-SFYQMNCMSMGGNPPATLKWF-----LSGSRKEIKANYTNHGSVAQSLLEMQAKREDNGKELRCEASNPAL---EGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVSRLTWLRGNGQVIQPAGNSTV-PGAYSGIVSKSTLQMEVTPELHGVVITCQATN-GIGPQIHDAITLEV------------LYKPQFQ-QPSDYSVDVIEGEPAIVNLTARANPSEMTYKW---FRDGTAIKQLKEANAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNVQYPASIVQARSTVMINEGSDAEFECRAAGNPLTTTTVFWRRAGFDMEGRTSQTPGVGVAYLLVREITRNDTGAFECVANNGIGQ-ESIEQTWLVVKYKPLMDDSPQLTKAAGDEGQLAKLVCRAQGAPNISFGWSREGTPLN-----------PSDKYSFSTRQVDIVTWESTMGVSNVRSRDYGLYDCIARNEMGTNSAKVVLSGTSRPDPPLALHVVNVTHDSVYLAWRSGFDGGLFQSYRLRYRQIGNEFYKYADVYPTNVTAFTLGGLALGTEYLFGIMAFNNLGESNYTQDIVKARTSS------PDDDMNADLLLTRGDSPKIIIIAVSTAGALLLVLNVALVLCYLIRRRKKKCMEEAMSSEG---GSSKSATIEMYAPSSYNETVNGETLSSISEKSESYS 1119          

HSP 2 Score: 150.599 bits (379), Expect = 4.097e-33
Identity = 173/676 (25.59%), Postives = 286/676 (42.31%), Query Frame = 0
Query:   52 DNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQ-ENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAV---MVVTGETAHFECVVD-AKPITADTISWSRVGFDM---EGRTSMQ---NGTNSMHLTVYNVSKEDAGEFL-CTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESE-LHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            D++  Y+C+       +P+ AD  L+V   P  V I          +  G+ L   C+  N+ P A + W    V+ +S + EN T   E  R  ++TRS +++   P+D      C A +  L      +  +++ YPPG P I G   G  +  GQ  +L CIS  GNPLA + WY+ +  I      D   ET       +H+  A  SDN   +RCEASN  T   + P+ + ITL+V F P T+ V + P   K      L C T +SNP A + W   DG  +   G  R+ + P GG ++++ L   V A    VT  C A   +L + V +   ++V        +    D+ I D    ++ + +         +S+  NP   T +W    + +   I  +  N   +   Q+LL +   R+++    + + +N   +  L     +        +T  V    +  G+     C  D + P++   ++W R    +    G +++    +G  S       V+ E  G  + C A NGI        T L V  +P+ ++ P++      + + A++   A+  P ++ + W R G+ I Q  E                N  DR+T++   L++  V   D G Y C A N EG   + V LNV
Sbjct:  104 DDDAEYQCQVGPRGLSKPIRADAQLTVLIPPSSVEIVDYVAGSRIMSREGEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRR-FDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKI------DHAYETTGRESRNVHIFRAEASDNNARFRCEASNALT---TTPMKAEITLTVHFGPKTVTV-TGPSEAKVNDTVSLECSTTNSNPAARITWVL-DGREVRENGS-RIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRD----NSFYQMN-------CMSMGGNP-PATLKWF--LSGSRKEIKANYTNHGSV--AQSLLEMQAKREDNGKELRCEASNPALEGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVS--RLTWLRGNGQVIQPAGNSTVPGAYSGIVSKSTLQMEVTPELHGVVITCQATNGIGPQIHDAIT-LEVLYKPQFQQ-PSDYSVDVIEGEPAIVNLTARANPSEMTYKWFRDGTAIKQLKEA---------------NAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNV 731          

HSP 3 Score: 81.6481 bits (200), Expect = 6.913e-12
Identity = 91/401 (22.69%), Postives = 168/401 (41.90%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQID---FSYYTTGRE------SRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTH-TISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW-YRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAY-LFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVK 387
            G+ + L C+     P AE+VW++ + +I        T+  E      +R+  T  A  +DN   Y C+A++   + P  A + L++Q+ P    I G TE     +G  +  +CI+   NP A + W  +       +      E  G E+R+        +D N    C A +N L  T ++   T++V + P   ++TG     + D   +  L C +   NP A +TW   G E+ +  ++     +    + S L  T   +   +   C A     AQ    ++ T  +SV + P   ++    +        Y + C +   NP A + W  +      ++ EI+       G++  ++L +  +  D+G    CEA+N  L  ++ D  T+ ++
Sbjct:  153 GEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRRFDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKIDHAY------ETTGRESRNVHIFRAEASDNNARFRCEA-SNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVND---TVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQR---VAETKIISVVYPPEAPSISGMRDRTIRDNSFYQMNCMSMGGNPPATLKWFLSG-----SRKEIKANYTNH-GSVAQSLLEMQAKREDNGKELRCEASNPALEGNLVDSTTIRIE 534          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|942323125|gb|JAN43495.1| (Down syndrome cell adhesion molecule [Daphnia magna])

HSP 1 Score: 658.677 bits (1698), Expect = 0.000e+0
Identity = 373/910 (40.99%), Postives = 534/910 (58.68%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQD--DDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNI-PRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQG-----IILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYT 902
            MGQ V L C+S GGNPLA++VWY+ + +ID +Y TTGRESRN++ F A + DNN R+RC+A N ++  P+ A+I L+V F P  VT+ GP+EA V DT+S +C TTNSNP A + WV+D R  +EN +    S  GGW + SN++  +    RN  +SC+AI  DL + V +T  ISV+YPP  PSI+G+   TI D     ++ C+SM GNP ATL W+     + G+ ++   + T   + AQS L + A   DNG   RCEASNPA     G L  + T+ + F P  + VK  PE  + G+   + CE+ SSNP + + W R +G  I+  G   V  G + G ++ + L ++V    HGV   C+ATN  +G  ++D IT+ V            + KP F   P+D    V EG   +VN++ RANP ++TY+W   + +      L   N   RI     LLN+T  R++D G YK    N+EG     ++++V Y A+I      VM+  G  A FEC     P+T  T+ W R GFDMEGRTS   G    +L V  +++ D G F C ANNGI   +  + T+L+V+ +P +++SP  +KAA D+ + A L CRAQG P++ F WSR G+ ++             +KY      +D +TWES + + NV SRDYG YDCIA+NE G +   V L+ TSRPD P  L+V N T++SVYL W  GFDGG  QS+ +RYR+ G++ Y+Y DV+ ++  +F +  L LGT+Y F + AFN+LG+S +T + V   T S        +D + +  +++G     II+ ++    LLL+LNV L+ CY+ RRR  +    +++  G   GS+KSATIEMYA SSYNET++GETLSSISEKS  Y+
Sbjct:  258 MGQEVKLECISRGGNPLAQIVWYKNEVKIDHAYETTGRESRNVHIFRAEASDNNARFRCEASNALTTTPMKAEITLTVHFGPKTVTVTGPSEAKVNDTVSLECSTTNSNPAARITWVLDGREVRENGSRIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRDN-SFYQMNCMSMGGNPPATLKWF-----LSGSRKEIKANYTNHGSVAQSLLEMQAKREDNGKELRCEASNPAL---EGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVSRLTWLRGNGQVIQPAGNSTV-PGAYSGIVSKSTLQMEVTPELHGVVITCQATN-GIGPQIHDAITLEV------------LYKPQFQ-QPSDYSVDVIEGEPAIVNLTARANPSEMTYKW---FRDGTAIKQLKEANAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNVQYPASIVQARSTVMINEGSDAEFECRAAGNPLTTTTVFWRRAGFDMEGRTSQTPGVGVAYLLVREITRNDTGAFECVANNGIGQ-ESIEQTWLVVKYKPLMDDSPQLTKAAGDEGQLAKLVCRAQGAPNISFGWSREGTPLN-----------PSDKYSFSTRQVDIVTWESTMGVSNVRSRDYGLYDCIARNEMGTNSAKVVLSGTSRPDPPLALHVVNVTHDSVYLAWRSGFDGGLFQSYRLRYRQIGNEFYKYADVYPTNVTAFTLGGLALGTEYLFGIMAFNNLGESNYTQDIVKARTSS------PDDDMNADLLLTRGDSPKIIIIAVSTAGALLLVLNVALVLCYLIRRRKKKCMEEAMSSEG---GSSKSATIEMYAPSSYNETVNGETLSSISEKSESYS 1119          

HSP 2 Score: 149.828 bits (377), Expect = 7.143e-33
Identity = 187/737 (25.37%), Postives = 305/737 (41.38%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFS--------YYTTGRESR---NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQWV---VDSRSAQ-ENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAV---MVVTGETAHFECVVD-AKPITADTISWSRVGFDM---EGRTSMQ---NGTNSMHLTVYNVSKEDAGEFL-CTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESE-LHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            GQ   L C    GN      W +    + F         Y   G E +   N+    A+ ED+ + Y+C+       +P+ AD  L+V   P  V I          +  G+ L   C+  N+ P A + W    V+ +S + EN T   E  R  ++TRS +++   P+D      C A +  L      +  +++ YPPG P I G   G  +  GQ  +L CIS  GNPLA + WY+ +  I      D   ET       +H+  A  SDN   +RCEASN  T   + P+ + ITL+V F P T+ V + P   K      L C T +SNP A + W   DG  +   G  R+ + P GG ++++ L   V A    VT  C A   +L + V +   ++V        +    D+ I D    ++ + +         +S+  NP   T +W    + +   I  +  N   +   Q+LL +   R+++    + + +N   +  L     +        +T  V    +  G+     C  D + P++   ++W R    +    G +++    +G  S       V+ E  G  + C A NGI        T L V  +P+ ++ P++      + + A++   A+  P ++ + W R G+ I Q  E                N  DR+T++   L++  V   D G Y C A N EG   + V LNV
Sbjct:   46 GQMAVLRC--KVGNQQGRAQWTKDGFALGFERTIPSFPRYTIMGSEEQGEHNIRIENATLEDDAE-YQCQVGPRGLSKPIRADAQLTVLIPPSSVEIVDYVAGSRIMSREGEELLLQCLVRNAKPAAEIVWFKRNVEIKSERIENTTSDAELPRR-FDTRSKLTIRAQPDDNEADYICEARHPALIAPKRASVALNIQYPPGPPEIIGYTEGETVRMGQEVKLECISRGGNPLAQIVWYKNEVKI------DHAYETTGRESRNVHIFRAEASDNNARFRCEASNALT---TTPMKAEITLTVHFGPKTVTV-TGPSEAKVNDTVSLECSTTNSNPAARITWVL-DGREVRENGS-RIATSPDGGWVSSSNLTTTVRATGRNVTVSCHAIVADLAQRVAETKIISVVYPPEAPSISGMRDRTIRD----NSFYQMN-------CMSMGGNP-PATLKWF--LSGSRKEIKANYTNHGSV--AQSLLEMQAKREDNGKELRCEASNPALEGNLVDSTTIRIEFAPKQLTVKVRPEKLRPGQMVTITCESDSSNPVS--RLTWLRGNGQVIQPAGNSTVPGAYSGIVSKSTLQMEVTPELHGVVITCQATNGIGPQIHDAIT-LEVLYKPQFQQ-PSDYSVDVIEGEPAIVNLTARANPSEMTYKWFRDGTAIKQLKEA---------------NAYDRITFDGPLLNLTVVRRDDKGEYKCDATNAEGSRSHTVRLNV 731          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|926606889|ref|XP_013782532.1| (PREDICTED: nephrin-like [Limulus polyphemus])

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 367/938 (39.13%), Postives = 546/938 (58.21%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDR-NKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRW---ANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTS--MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQG----------IILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKS---------GXYTSSSVDPIHGHH 913
            G T++L CVS GGNPLA++VW++ ++Q+DFSY T+GR+S N +TFV  + DNN  YRC+A N +S +P+ A + LSV FA  +V + GP EA  G+ ++  C T+ SNP A + W+VD +  +   T+  +   GGW T+SN++V+I   +R ++I  C+A+N +LGET V++H +S+LYPP  P+I G   G  +  G+++++TC S  GNPL  L W+R DE ++  +     T + N   SE+ +     DNG+ YRCEASN A+ +   PL S I L+V F P  + +K  P   KAG K  L CE+ SSNP + + W RN  L    QG    +   P GG  T N+L ++V + D G  + C+A NE L  SV+  +T+ +  K    P    + K I+DI          EG+   +N++ R NP  +TY W     P  +   +  L++     + S   +L IT   + ++GRYK +  N EG S   I ++VLY ATIS +T   MV     A+ ECV  A P+T + I W R+ FDM  RT   ++NG +   LT+YNVS+ED+G F C A+NGI   K  K ++L+V+ +P I++S    KAAS+K +TA L CR  G P++ F+WS  G+ +S          I   KY    + +D +TWES L++K+V + DYG Y+C+A+N+ G     + LN TS+PD P  + V N  +N+  L W PGFDGG  Q++ IRY++ G + YQY DVH  +   + +  L +G  Y+ S++AFN++G SEFT++ V V+T S +       D + ER I++           II+++++V   LL LNV+L+ C+V+RRR  R+      E  S   S+K+ATIEMYA SSYN+T++GET+SS SEKS         G Y+   V P+   +
Sbjct:  236 GDTITLSCVSRGGNPLAQLVWFKNNQQVDFSYTTSGRKSTNKHTFVVDTSDNNAIYRCEASNIISPQPMNAVVKLSVLFASSEVKVSGPKEAKPGEAVTLSCATSRSNPAAEINWLVDGQHLKGTNTISADP-NGGWTTKSNVTVIIELQERHSRIFQCFAVNKELGETKVESHVLSILYPPDPPTILGFTKGRSIRAGETQKVTCASTGGNPLPALKWFREDEEVKSVS-----TTSGNVVSSEVTIETKERDNGVEYRCEASNSASTK---PLISEIKLTVHFPPSGVTIKVKPPKQKAGGKVNLICESASSNPASLITWWRNGFLL---QGSPSGIFDAPHGGKSTRNLLQLNVTSEDDGSRYTCQAMNEVLERSVHSTVTLNITYK----PEFYKLQKEIYDI---------VEGNSTFINLTARGNPNFITYNWVKDGKPVVDIQVDRELNNFKCSGVCSQGPILRITNVTRTNSGRYKCEATNEEGTSETTIILNVLYPATISKVTQVTMVEQSTNAYLECVAGANPLTDNMIFWRRLDFDMN-RTKHYLENGHS--FLTIYNVSQEDSGAFQCVADNGIGP-KHLKESYLVVKHKPIIDKSSPLLKAASEKGETAKLICRGNGAPNITFTWSHEGATVSGS--------IRPVKYFTGVSKLDLITWESILYVKDVKNHDYGVYECVARNDLGFDSVKITLNSTSQPDPPIGMKVLNVDHNTAMLKWNPGFDGGLPQAYRIRYKENGYEAYQYADVHPPNATVYNLTGLGVGMDYTLSIQAFNEMGASEFTTDIVKVKTSSEV------PDAETERAITKVLSEKGELPRIIIVSVSIVGTFLLALNVVLVICFVRRRRKKRL------EEESDQTSSKAATIEMYAPSSYNDTVNGETMSSTSEKSEGYSDGPSTGEYSEEPVKPVLAKY 1124          

HSP 2 Score: 160.999 bits (406), Expect = 2.508e-36
Identity = 170/696 (24.43%), Postives = 300/696 (43.10%), Query Frame = 0
Query:   42 NMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGD-----TLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTES--------ERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARD-HGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL-------VVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLY---GATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSM-----QNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVV-FSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
            N+    A  ED+ + Y+C+   T     + A+  L+V   P  V I      +V +     +LS  C      P A ++W       ++N  +R ++        ++  + T S I++   P+D   I +C A+++ L + +  +  +SVL+PPG P+I G   G  +  G +  L+C+S  GNPLA L W++ ++ +     D   T +   + ++     + SDN  +YRCEASN  + Q   P+++ + LSV F    + V S P+  K G+   L C T  SNP A + W   DG  +  +G   + + P GG  T + + + +E ++ H   F C A N+ELGE+  +   +++                ++  DP  TI    +G  +       V   S   NP     +W    +E + +++  S N+     + + + I    +++   Y+ + +NS     L  +IK+ V +   G TI           G   +  C   A    A  I+W R GF ++G  S        G ++ +L   NV+ ED G  + C A N + +     +  L +  +P   +         +   T  +   A+G P+ + ++W + G  +          +I+ ++    +      +    L I NV+  + G Y C A NEEG S   + LNV
Sbjct:   73 NLLIKNARLEDDGE-YQCQVGPTPDNHAIRANARLNVLVPPTSVVISDYENGSVVEIHQLQSLSLTCTVIGGKPAAHIKWF------RKNVELRPDNVETKTKEEKKQKFNTVSTITLTPNPDDNGAIYTCQAVHHALVKPLRSSVILSVLFPPGPPAIDGFQAGQAVRSGDTITLSCVSRGGNPLAQLVWFKNNQQV-----DFSYTTSGRKSTNKHTFVVDTSDNNAIYRCEASNIISPQ---PMNAVVKLSVLFASSEVKV-SGPKEAKPGEAVTLSCATSRSNPAAEINWLV-DGQHL--KGTNTISADPNGGWTTKSNVTVIIELQERHSRIFQCFAVNKELGETKVESHVLSI----------------LYPPDP-PTILGFTKGRSIRAGETQKVTCASTGGNPLPA-LKWFRE-DEEVKSVSTTSGNV-----VSSEVTIETKERDNGVEYRCEASNSASTKPLISEIKLTVHFPPSGVTIK--VKPPKQKAGGKVNLIC-ESASSNPASLITWWRNGFLLQGSPSGIFDAPHGGKSTRNLLQLNVTSEDDGSRYTCQAMNEVLERSVHSTVTLNITYKPEFYKLQKEIYDIVEGNST-FINLTARGNPNFITYNWVKDGKPVV---------DIQVDRELNNFKCSGVCSQGPILRITNVTRTNSGRYKCEATNEEGTSETTIILNV 712          

HSP 3 Score: 114.005 bits (284), Expect = 8.108e-22
Identity = 156/744 (20.97%), Postives = 286/744 (38.44%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQI--------DFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSD-NGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEE------GSDLVVNISVRANPFDVTYRWANPY-NENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNS--EGKSRLKIKIDVLYG-------ATISDITDAVMVVTGETAH-------FECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYA-VYLNVTSRP 708
            Q++SL C   GG P A + W+R + ++                  + +  T   + +DN   Y C+A +   V+PL + ++LSV F P    I+G         GDT++  C++   NP A + W  +++    ++T        G ++ +  + ++  +D N I  C A N    + +     +SVL+   +  ++G         G++  L+C +   NP A + W    + ++GT     +       +S + +     + +  +++C A N     E+   S  +++     P TI   +     +AG+   + C +   NP   + W R D    E    +   S    GN+ ++ + I+ + RD+GV + CEA+N    + +   I +TV                     P+     V+       G   ++  S  +NP  +   W N +  +  P+   D+ +  +  S +NLL +    ++D  RY  +  N   E      + +++ Y          I DI +        TA        +  V D KP+    +      F   G  S         L + NV++ ++G + C A N   +   +++T +L    P    S        ++   A L C A   P  D +  W R+   +++     E+                     S L I NVS  D G++ C+A N  G       YL V  +P
Sbjct:  132 QSLSLTCTVIGGKPAAHIKWFRKNVELRPDNVETKTKEEKKQKFNTVSTITLTPNPDDNGAIYTCQAVHHALVKPLRSSVILSVLFPPGPPAIDGFQAGQAVRSGDTITLSCVSRGGNPLAQLVWFKNNQQVDFSYTT------SGRKSTNKHTFVVDTSDNNAIYRCEASNIISPQPMNAVVKLSVLFASSEVKVSGPKEA---KPGEAVTLSCATSRSNPAAEINWLVDGQHLKGTNTISADPNGGWTTKSNVTVIIELQERHSRIFQCFAVNKELG-ETKVESHVLSILYPPDPPTILGFTKGRSIRAGETQKVTCASTGGNPLPALKWFRED----EEVKSVSTTS----GNVVSSEVTIETKERDNGVEYRCEASNSASTKPLISEIKLTVH------------------FPPSGVTIKVKPPKQKAGGKVNLICESASSNPASLITWWRNGFLLQGSPSGIFDAPHGGK--STRNLLQLNVTSEDDGSRYTCQAMNEVLERSVHSTVTLNITYKPEFYKLQKEIYDIVEGNSTFINLTARGNPNFITYNWVKDGKPVVDIQVDRELNNFKCSGVCSQ-----GPILRITNVTRTNSGRYKCEATN---EEGTSETTIILNVLYPAT-ISKVTQVTMVEQSTNAYLECVAGANPLTDNMIFWRRLDFDMNRTKHYLENG-------------------HSFLTIYNVSQEDSGAFQCVADNGIGPKHLKESYLVVKHKP 809          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|1008431230|ref|XP_015833149.1| (PREDICTED: nephrin isoform X1 [Tribolium castaneum])

HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0
Identity = 357/910 (39.23%), Postives = 540/910 (59.34%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNI-TLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGF-DMEGRTSM---QNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK--PEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTS 903
            GQTV L+C S GGNP A+++WY+  EQI  +Y T GR S N+YTF A + DN  RY+C+A N +S  PL A++ ++V F+P  VTI GPTEA VGD +   C T NSNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I G   G+ +  G  ++++C S  GNPLATLTWY+ D+ I    +  D++ T     +E+ +  N +DN   YRCEA+N AT     PL  TIT++V F P  + ++ +P   K  ++A L C++ SSNP A + W R +G+ +  QG +   + G  GG +++  L +++  + +G+ + C+ATNE L  SV+D IT+ V            + KP+FD D  + +  + EG  L++ +    NP ++ Y W     + +P I +  S  I RI S   +LNIT   + DAG Y  +  NS+G S  ++ I V Y ATI   ++ V+V   E A   C  D  P++ DTI+W R  F D + RTS+   +NGT+ + +T  +V+++D G F C  NNG+ ++  TK   L+V+ +P I++ PA  K ASD   T  + CR+Q +P   + W+R G+ I+              KY   Y  ID +T ES L I +V+S DYG+Y+C+A+NE G +  +  L VTS PDTPTLL + N T+++V L W PGFDGG K ++ IRYR+     Y+YVDV   +  SF + DL++ T+Y FS+ A N LG+S++  + ++  T  Y + + K  PED   ++ + + +I++++++   LLI+N+ L++  + +RR  R+   S       N ++KSATIEMYA SSYN+T++GETLSS+SEKS  Y++
Sbjct:  259 GQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARVGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKINSVLKTTDKSVT-----AEITILTNVTDNEARYRCEAANSAT---EIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAKLSWWR-EGIPV--QGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQV------------LYKPVFDKDNEEAVTGI-EGEPLIITLKADGNPQNIAYTWT---KDGLPIIQSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITVQYAATIVATSENVVVNPNEDATLSCTADGNPLSDDTITWKRDDFPDFDARTSVMYDKNGTSYLRIT--DVTRDDLGNFQCIVNNGVGNVT-TKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAKYKWARSGAPITAN---------TTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELGFATASPRLEVTSAPDTPTLLTILNVTHDTVTLGWTPGFDGGMKAAYRIRYRQVNDDGYKYVDVVPQNATSFLIRDLEVNTQYVFSIMAMNKLGNSKYMPDLLSARTSMYSDHIEKIIPEDLLEKQDLPRIVIISVSVIGIFLLIVNIGLVAGCMLKRRAKRIREQS-------NQTSKSATIEMYAPSSYNDTVTGETLSSVSEKSETYSN 1122          

HSP 2 Score: 123.635 bits (309), Expect = 8.607e-25
Identity = 178/735 (24.22%), Postives = 282/735 (38.37%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQW----VVDSRSAQENFTVRTESERGG-----WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL----VVNISVRA---NPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASD-KRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
             N   +V W +    + FS        Y   G     +Y    S  S +++  Y+C+       + + A+  L+V   P  V I         E         +C   N+ P A + W    V  +   +E+  +    + G      ++T S IS+     D     +C A +  L   +    T+  SV YPPG P I G   G  +  GQ+  L C S  GNP A L WY+  E I+   +         Y       TA+ SDN   Y+CEASN        PL + + ++V F P  + + S P   + G    L C T +SNP A + W    G  + N    R    P GG +TT+ +   VE     +  IC   N +L E+V    T+ V       P   F             I    +GS +    V  IS  +   NP   T  W    N+   N  L + +      I  L N+T    ++  RY+ +  NS  +  L   I ++V +      I  + + +   E A   C   +    A  +SW R G  ++G     +  +  GT S      N++++  G  + C A N         +  L V  +P  ++   N +A +  + +  ++T +A G P ++ ++W++ G  I Q       E I  +                 L+I  +S  D G+Y C A N +G S   V + V
Sbjct:   48 ANLAGQVQWTKDGFALGFSSVIPGFPRYSVIGDRRHGIYNLRVSNASLEDDAEYQCQVGPARLHKAIRANARLNVISPPSSVEILDHRHNSKIEIKENQEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYT-----FTADASDNKARYKCEASN---IMSKAPLKAEVDMTVLFSPAHVTI-SGPTEARVGDPVPLTCTTANSNPPAEIKWMV-AGRQVRNATS-RTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINV----LYPPSAPF-------------IHGYTQGSYIPAGTVQKISCTSSGGNPL-ATLTWYK--NDKKINSVLKTTDKSVTAEITILTNVT----DNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAK-LSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKD--NEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDG--------------PVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITV 730          

HSP 3 Score: 92.4337 bits (228), Expect = 2.925e-15
Identity = 170/755 (22.52%), Postives = 288/755 (38.15%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDF---------------SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE---VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATI-SDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSM-----HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRPDT---PTLL 714
            Q   L C      P A++VWYRG+ +++                    T  ++ +  +   ++ED+   Y C+A++       PL   + LSV + P    IEG TE      G T+   C +   NPPA + W  +         +R      G  + +  +     +D      C A N      +     ++VL+ P   +I+G     +   G    LTC +   NP A + W   G ++   T++     E      S +      +   +V  C   N    Q +  + ST T++V + P    +    +   +P AG    + C +   NP A + W +ND   I +  +   +S      + TNV   D EAR     + CEA N      + + ITM V    +  P    I K   ++ P        E + L  + S    P  +++     + E IP   L +   P ++     SI+  LNITE  + +   Y  +  N   +  +   I + VLY      D  +AV  + GE        D  P      +W++ G  +  ++S  +G   +      L +  +S+ DAG + C A N  +         + V+    I  +  N     +  + A L+C A G P  D   +W R              ++  D  ++ + +V+      S L I +V+  D G++ CI  N  G ++   V L V  +P+    P LL
Sbjct:  146 QEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQ------IRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARV---GDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLN---MQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIP-AGTVQKISCTSSGGNPLATLTWYKNDK-KINSVLKTTDKSVTAEITILTNV--TDNEAR-----YRCEAANSATEIPLFETITMNV----YFPPDHVKIRKEPLELKP-------NEQATLTCDSSSSNPPAKLSW-----WREGIPVQGLLNTTKPGLHGGTVSSIELKLNITE--QLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNI-AYTWTKDGLPII-QSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALN--SQGSSVAQVNITVQYAATIVATSEN--VVVNPNEDATLSCTADGNPLSDDTITWKR--------------DDFPD--FDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALL 839          

HSP 4 Score: 88.9669 bits (219), Expect = 3.990e-14
Identity = 142/614 (23.13%), Postives = 238/614 (38.76%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG---TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSI----ENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQN---LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV---TGETAHFECVVDAKPITADTISWSRVGFDMEGRTS---------MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            +G  V L C +   NP AE+ W     Q+  +   T      G  + +  T V      +    C   N    E + +   ++V + P    I G T+ +    G      C ++  NP A + W  + +    N  ++T  +       + I++L    D      C A N+     + +T T++V +PP    I   +P  +    Q+  LTC S + NP A L+W+R    +QG   TT+      T +  + +L++T     NG+VY C+A+N A  +    +   ITL V +KP+      +      G+   +  +   +       W + DGL I     + G  R+ S GP        VL I   +R    T+ CEA N + G SV   + +TV+  +               I  T     V    D  ++ +   NP  D T  W     ++ P    D D    +   +N    L IT+  ++D G ++  VNN  G    K  + ++      D   A++      G+T    C   A P+      W+R G  +   T+         +   T+   L + +V+  D G + C A N + 
Sbjct:  352 VGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKI--NSVLKTTDK----SVTAEITILTNVTDNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRK-EPLELKPNEQA-TLTCDSSSSNPPAKLSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQL--NGIVYTCQATNEALQRS---VHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNIAYTWTK-DGLPIIQSSSSSGIERIISDGP--------VLNITRLSRHDAGTYSCEALNSQ-GSSVAQ-VNITVQYAA--------------TIVATSENVVVNPNEDATLSCTADGNPLSDDTITWK---RDDFP----DFDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAK--YKWARSGAPITANTTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELG 918          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|642915658|ref|XP_008190700.1| (PREDICTED: nephrin isoform X3 [Tribolium castaneum] >gi|1004402240|gb|KYB28870.1| Nephrin-like Protein [Tribolium castaneum])

HSP 1 Score: 642.114 bits (1655), Expect = 0.000e+0
Identity = 357/910 (39.23%), Postives = 540/910 (59.34%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNI-TLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGF-DMEGRTSM---QNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK--PEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTS 903
            GQTV L+C S GGNP A+++WY+  EQI  +Y T GR S N+YTF A + DN  RY+C+A N +S  PL A++ ++V F+P  VTI GPTEA VGD +   C T NSNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I G   G+ +  G  ++++C S  GNPLATLTWY+ D+ I    +  D++ T     +E+ +  N +DN   YRCEA+N AT     PL  TIT++V F P  + ++ +P   K  ++A L C++ SSNP A + W R +G+ +  QG +   + G  GG +++  L +++  + +G+ + C+ATNE L  SV+D IT+ V            + KP+FD D  + +  + EG  L++ +    NP ++ Y W     + +P I +  S  I RI S   +LNIT   + DAG Y  +  NS+G S  ++ I V Y ATI   ++ V+V   E A   C  D  P++ DTI+W R  F D + RTS+   +NGT+ + +T  +V+++D G F C  NNG+ ++  TK   L+V+ +P I++ PA  K ASD   T  + CR+Q +P   + W+R G+ I+              KY   Y  ID +T ES L I +V+S DYG+Y+C+A+NE G +  +  L VTS PDTPTLL + N T+++V L W PGFDGG K ++ IRYR+     Y+YVDV   +  SF + DL++ T+Y FS+ A N LG+S++  + ++  T  Y + + K  PED   ++ + + +I++++++   LLI+N+ L++  + +RR  R+   S       N ++KSATIEMYA SSYN+T++GETLSS+SEKS  Y++
Sbjct:  258 GQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARVGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKINSVLKTTDKSVT-----AEITILTNVTDNEARYRCEAANSAT---EIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAKLSWWR-EGIPV--QGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQV------------LYKPVFDKDNEEAVTGI-EGEPLIITLKADGNPQNIAYTWT---KDGLPIIQSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITVQYAATIVATSENVVVNPNEDATLSCTADGNPLSDDTITWKRDDFPDFDARTSVMYDKNGTSYLRIT--DVTRDDLGNFQCIVNNGVGNVT-TKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAKYKWARSGAPITAN---------TTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELGFATASPRLEVTSAPDTPTLLTILNVTHDTVTLGWTPGFDGGMKAAYRIRYRQVNDDGYKYVDVVPQNATSFLIRDLEVNTQYVFSIMAMNKLGNSKYMPDLLSARTSMYSDHIEKIIPEDLLEKQDLPRIVIISVSVIGIFLLIVNIGLVAGCMLKRRAKRIREQS-------NQTSKSATIEMYAPSSYNDTVTGETLSSVSEKSETYSN 1121          

HSP 2 Score: 123.635 bits (309), Expect = 8.977e-25
Identity = 178/735 (24.22%), Postives = 282/735 (38.37%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQW----VVDSRSAQENFTVRTESERGG-----WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL----VVNISVRA---NPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASD-KRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
             N   +V W +    + FS        Y   G     +Y    S  S +++  Y+C+       + + A+  L+V   P  V I         E         +C   N+ P A + W    V  +   +E+  +    + G      ++T S IS+     D     +C A +  L   +    T+  SV YPPG P I G   G  +  GQ+  L C S  GNP A L WY+  E I+   +         Y       TA+ SDN   Y+CEASN        PL + + ++V F P  + + S P   + G    L C T +SNP A + W    G  + N    R    P GG +TT+ +   VE     +  IC   N +L E+V    T+ V       P   F             I    +GS +    V  IS  +   NP   T  W    N+   N  L + +      I  L N+T    ++  RY+ +  NS  +  L   I ++V +      I  + + +   E A   C   +    A  +SW R G  ++G     +  +  GT S      N++++  G  + C A N         +  L V  +P  ++   N +A +  + +  ++T +A G P ++ ++W++ G  I Q       E I  +                 L+I  +S  D G+Y C A N +G S   V + V
Sbjct:   47 ANLAGQVQWTKDGFALGFSSVIPGFPRYSVIGDRRHGIYNLRVSNASLEDDAEYQCQVGPARLHKAIRANARLNVISPPSSVEILDHRHNSKIEIKENQEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYT-----FTADASDNKARYKCEASN---IMSKAPLKAEVDMTVLFSPAHVTI-SGPTEARVGDPVPLTCTTANSNPPAEIKWMV-AGRQVRNATS-RTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINV----LYPPSAPF-------------IHGYTQGSYIPAGTVQKISCTSSGGNPL-ATLTWYK--NDKKINSVLKTTDKSVTAEITILTNVT----DNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAK-LSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKD--NEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDG--------------PVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITV 729          

HSP 3 Score: 92.4337 bits (228), Expect = 2.949e-15
Identity = 170/755 (22.52%), Postives = 288/755 (38.15%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDF---------------SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE---VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATI-SDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSM-----HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRPDT---PTLL 714
            Q   L C      P A++VWYRG+ +++                    T  ++ +  +   ++ED+   Y C+A++       PL   + LSV + P    IEG TE      G T+   C +   NPPA + W  +         +R      G  + +  +     +D      C A N      +     ++VL+ P   +I+G     +   G    LTC +   NP A + W   G ++   T++     E      S +      +   +V  C   N    Q +  + ST T++V + P    +    +   +P AG    + C +   NP A + W +ND   I +  +   +S      + TNV   D EAR     + CEA N      + + ITM V    +  P    I K   ++ P        E + L  + S    P  +++     + E IP   L +   P ++     SI+  LNITE  + +   Y  +  N   +  +   I + VLY      D  +AV  + GE        D  P      +W++ G  +  ++S  +G   +      L +  +S+ DAG + C A N  +         + V+    I  +  N     +  + A L+C A G P  D   +W R              ++  D  ++ + +V+      S L I +V+  D G++ CI  N  G ++   V L V  +P+    P LL
Sbjct:  145 QEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQ------IRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARV---GDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLN---MQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIP-AGTVQKISCTSSGGNPLATLTWYKNDK-KINSVLKTTDKSVTAEITILTNV--TDNEAR-----YRCEAANSATEIPLFETITMNV----YFPPDHVKIRKEPLELKP-------NEQATLTCDSSSSNPPAKLSW-----WREGIPVQGLLNTTKPGLHGGTVSSIELKLNITE--QLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNI-AYTWTKDGLPII-QSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALN--SQGSSVAQVNITVQYAATIVATSEN--VVVNPNEDATLSCTADGNPLSDDTITWKR--------------DDFPD--FDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALL 838          

HSP 4 Score: 88.5817 bits (218), Expect = 4.379e-14
Identity = 145/616 (23.54%), Postives = 237/616 (38.47%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG---TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKA--YLFCETGSSNPTANVIWRRNDGLSI----ENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQN---LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV---TGETAHFECVVDAKPITADTISWSRVGFDMEGRTS---------MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            +G  V L C +   NP AE+ W     Q+  +   T      G  + +  T V      +    C   N    E + +   ++V + P    I G T+ +    G      C ++  NP A + W  + +    N  ++T  +       + I++L    D      C A N+     + +T T++V +PP    I   +P  +    Q+  LTC S + NP A L+W+R    +QG   TT+      T +  + +L++T     NG+VY C+A+N A  +    +   ITL V +KP+      D E    G +    +       NP         DGL I     + G  R+ S GP        VL I   +R    T+ CEA N + G SV   + +TV+  +               I  T     V    D  ++ +   NP  D T  W     ++ P    D D    +   +N    L IT+  ++D G ++  VNN  G    K  + ++      D   A++      G+T    C   A P+      W+R G  +   T+         +   T+   L + +V+  D G + C A N + 
Sbjct:  351 VGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKI--NSVLKTTDK----SVTAEITILTNVTDNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRK-EPLELKPNEQA-TLTCDSSSSNPPAKLSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQL--NGIVYTCQATNEALQRS---VHDAITLQVLYKPV---FDKDNEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDGP--------VLNITRLSRHDAGTYSCEALNSQ-GSSVAQ-VNITVQYAA--------------TIVATSENVVVNPNEDATLSCTADGNPLSDDTITWK---RDDFP----DFDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAK--YKWARSGAPITANTTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELG 917          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|1008431235|ref|XP_015833152.1| (PREDICTED: nephrin isoform X4 [Tribolium castaneum])

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 357/910 (39.23%), Postives = 540/910 (59.34%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNI-TLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGF-DMEGRTSM---QNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVK--PEDKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTS 903
            GQTV L+C S GGNP A+++WY+  EQI  +Y T GR S N+YTF A + DN  RY+C+A N +S  PL A++ ++V F+P  VTI GPTEA VGD +   C T NSNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I G   G+ +  G  ++++C S  GNPLATLTWY+ D+ I    +  D++ T     +E+ +  N +DN   YRCEA+N AT     PL  TIT++V F P  + ++ +P   K  ++A L C++ SSNP A + W R +G+ +  QG +   + G  GG +++  L +++  + +G+ + C+ATNE L  SV+D IT+ V            + KP+FD D  + +  + EG  L++ +    NP ++ Y W     + +P I +  S  I RI S   +LNIT   + DAG Y  +  NS+G S  ++ I V Y ATI   ++ V+V   E A   C  D  P++ DTI+W R  F D + RTS+   +NGT+ + +T  +V+++D G F C  NNG+ ++  TK   L+V+ +P I++ PA  K ASD   T  + CR+Q +P   + W+R G+ I+              KY   Y  ID +T ES L I +V+S DYG+Y+C+A+NE G +  +  L VTS PDTPTLL + N T+++V L W PGFDGG K ++ IRYR+     Y+YVDV   +  SF + DL++ T+Y FS+ A N LG+S++  + ++  T  Y + + K  PED   ++ + + +I++++++   LLI+N+ L++  + +RR  R+   S       N ++KSATIEMYA SSYN+T++GETLSS+SEKS  Y++
Sbjct:  259 GQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARVGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKINSVLKTTDKSVT-----AEITILTNVTDNEARYRCEAANSATEI---PLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAKLSWWR-EGIPV--QGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQV------------LYKPVFDKDNEEAVTGI-EGEPLIITLKADGNPQNIAYTWT---KDGLPIIQSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITVQYAATIVATSENVVVNPNEDATLSCTADGNPLSDDTITWKRDDFPDFDARTSVMYDKNGTSYLRIT--DVTRDDLGNFQCIVNNGVGNVT-TKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAKYKWARSGAPITAN---------TTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELGFATASPRLEVTSAPDTPTLLTILNVTHDTVTLGWTPGFDGGMKAAYRIRYRQVNDDGYKYVDVVPQNATSFLIRDLEVNTQYVFSIMAMNKLGNSKYMPDLLSARTSMYSDHIEKIIPEDLLEKQDLPRIVIISVSVIGIFLLIVNIGLVAGCMLKRRAKRIREQS-------NQTSKSATIEMYAPSSYNDTVTGETLSSVSEKSETYSN 1122          

HSP 2 Score: 122.865 bits (307), Expect = 1.381e-24
Identity = 178/735 (24.22%), Postives = 282/735 (38.37%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQW----VVDSRSAQENFTVRTESERGG-----WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDL----VVNISVRA---NPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASD-KRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
             N   +V W +    + FS        Y   G     +Y    S  S +++  Y+C+       + + A+  L+V   P  V I         E         +C   N+ P A + W    V  +   +E+  +    + G      ++T S IS+     D     +C A +  L   +    T+  SV YPPG P I G   G  +  GQ+  L C S  GNP A L WY+  E I+   +         Y       TA+ SDN   Y+CEASN        PL + + ++V F P  + + S P   + G    L C T +SNP A + W    G  + N    R    P GG +TT+ +   VE     +  IC   N +L E+V    T+ V       P   F             I    +GS +    V  IS  +   NP   T  W    N+   N  L + +      I  L N+T    ++  RY+ +  NS  +  L   I ++V +      I  + + +   E A   C   +    A  +SW R G  ++G     +  +  GT S      N++++  G  + C A N         +  L V  +P  ++   N +A +  + +  ++T +A G P ++ ++W++ G  I Q       E I  +                 L+I  +S  D G+Y C A N +G S   V + V
Sbjct:   48 ANLAGQVQWTKDGFALGFSSVIPGFPRYSVIGDRRHGIYNLRVSNASLEDDAEYQCQVGPARLHKAIRANARLNVISPPSSVEILDHRHNSKIEIKENQEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYT-----FTADASDNKARYKCEASN---IMSKAPLKAEVDMTVLFSPAHVTI-SGPTEARVGDPVPLTCTTANSNPPAEIKWMV-AGRQVRNATS-RTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINV----LYPPSAPF-------------IHGYTQGSYIPAGTVQKISCTSSGGNPL-ATLTWYK--NDKKINSVLKTTDKSVTAEITILTNVT----DNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAK-LSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKD--NEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDG--------------PVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITV 730          

HSP 3 Score: 91.6633 bits (226), Expect = 4.819e-15
Identity = 171/755 (22.65%), Postives = 285/755 (37.75%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDF---------------SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE---VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATI-SDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSM-----HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRPDT---PTLL 714
            Q   L C      P A++VWYRG+ +++                    T  ++ +  +   ++ED+   Y C+A++       PL   + LSV + P    IEG TE      G T+   C +   NPPA + W  +         +R      G  + +  +     +D      C A N      +     ++VL+ P   +I+G     +   G    LTC +   NP A + W   G ++   T++     E      S +      +   +V  C   N    Q +  + ST T++V + P    +    +   +P AG    + C +   NP A + W +ND   I +  +   +S      + TNV   D EAR     + CEA N      + + ITM V    +  P    I K   ++ P        E + L  + S    P  +++     + E IP   L +   P ++     SI+  LNITE  + +   Y  +  N   +  +   I + VLY      D  +AV  + GE        D  P      +W++ G  +  ++S  +G   +      L +  +S+ DAG + C A N  +         + V+    I  +  N     +  + A L+C A G P  D   +W R        P+ D    +  +K    Y           L I +V+  D G++ CI  N  G ++   V L V  +P+    P LL
Sbjct:  146 QEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQ------IRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARV---GDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLN---MQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIP-AGTVQKISCTSSGGNPLATLTWYKNDK-KINSVLKTTDKSVTAEITILTNV--TDNEAR-----YRCEAANSATEIPLFETITMNV----YFPPDHVKIRKEPLELKP-------NEQATLTCDSSSSNPPAKLSW-----WREGIPVQGLLNTTKPGLHGGTVSSIELKLNITE--QLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNI-AYTWTKDGLPII-QSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALN--SQGSSVAQVNITVQYAATIVATSEN--VVVNPNEDATLSCTADGNPLSDDTITWKR-----DDFPDFDARTSVMYDKNGTSY-----------LRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALL 839          

HSP 4 Score: 88.1965 bits (217), Expect = 6.525e-14
Identity = 145/616 (23.54%), Postives = 237/616 (38.47%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG---TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKA--YLFCETGSSNPTANVIWRRNDGLSI----ENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQN---LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV---TGETAHFECVVDAKPITADTISWSRVGFDMEGRTS---------MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            +G  V L C +   NP AE+ W     Q+  +   T      G  + +  T V      +    C   N    E + +   ++V + P    I G T+ +    G      C ++  NP A + W  + +    N  ++T  +       + I++L    D      C A N+     + +T T++V +PP    I   +P  +    Q+  LTC S + NP A L+W+R    +QG   TT+      T +  + +L++T     NG+VY C+A+N A  +    +   ITL V +KP+      D E    G +    +       NP         DGL I     + G  R+ S GP        VL I   +R    T+ CEA N + G SV   + +TV+  +               I  T     V    D  ++ +   NP  D T  W     ++ P    D D    +   +N    L IT+  ++D G ++  VNN  G    K  + ++      D   A++      G+T    C   A P+      W+R G  +   T+         +   T+   L + +V+  D G + C A N + 
Sbjct:  352 VGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKI--NSVLKTTDK----SVTAEITILTNVTDNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRK-EPLELKPNEQA-TLTCDSSSSNPPAKLSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQL--NGIVYTCQATNEALQRS---VHDAITLQVLYKPV---FDKDNEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDGP--------VLNITRLSRHDAGTYSCEALNSQ-GSSVAQ-VNITVQYAA--------------TIVATSENVVVNPNEDATLSCTADGNPLSDDTITWK---RDDFP----DFDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAK--YKWARSGAPITANTTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELG 918          
BLAST of EMLSAG00000004534 vs. nr
Match: gi|1008431232|ref|XP_015833150.1| (PREDICTED: nephrin isoform X2 [Tribolium castaneum])

HSP 1 Score: 638.647 bits (1646), Expect = 0.000e+0
Identity = 359/914 (39.28%), Postives = 534/914 (58.42%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIR-VRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNI-TLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGF-DMEGRTSM---QNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPE------DKDVERTISQGIILTITLVSGLLLILNVILISCYVKRRRIIRMNGSSVAEGGSTNGSTKSATIEMYAGSSYNETMSGETLSSISEKSGXYTS 903
            GQTV L+C S GGNP A+++WY+  EQI  +Y T GR S N+YTF A + DN  RY+C+A N +S  PL A++ ++V F+P  VTI GPTEA VGD +   C T NSNPPA ++W+V  R  +   +    S  GGW T SNI+ ++ PN R+ ++ C+ +N  L E VV THTI+VLYPP  P I G   G+ +  G  ++++C S  GNPLATLTWY+ D+ I    +  D++ T     +E+ +  N +DN   YRCEA+N AT     PL  TIT++V F P  + ++ +P   K  ++A L C++ SSNP A + W R +G+ +  QG +   + G  GG +++  L +++  + +G+ + C+ATNE L  SV+D IT+ V            + KP+FD D  + +  + EG  L++ +    NP ++ Y W     + +P I +  S  I RI S   +LNIT   + DAG Y  +  NS+G S  ++ I V Y ATI   ++ V+V   E A   C  D  P++ DTI+W R  F D + RTS+   +NGT+ + +T  +V+++D G F C  NNG+ ++  TK   L+V+ +P I++ PA  K ASD   T  + CR+Q +P   + W+R G+ I+              KY   Y  ID +T ES L I +V+S DYG+Y+C+A+NE G +  +  L VTS PDTPTLL + N T+++V L W PGFDGG K ++ IRYR+     Y+YVDV   +  SF + DL++ T+Y FS+ A N LG+S++  + ++  T       V P       D+   R     +++  T+V  LL+++NV+LI C + RR   R++  S       N ++KSATIEMYA SSYN+T++GETLSS+SEKS  Y++
Sbjct:  259 GQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARVGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKINSVLKTTDKSVT-----AEITILTNVTDNEARYRCEAANSATEI---PLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAKLSWWR-EGIPV--QGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQV------------LYKPVFDKDNEEAVTGI-EGEPLIITLKADGNPQNIAYTWT---KDGLPIIQSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITVQYAATIVATSENVVVNPNEDATLSCTADGNPLSDDTITWKRDDFPDFDARTSVMYDKNGTSYLRIT--DVTRDDLGNFQCIVNNGVGNVT-TKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAKYKWARSGAPITAN---------TTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELGFATASPRLEVTSAPDTPTLLTILNVTHDTVTLGWTPGFDGGMKAAYRIRYRQVNDDGYKYVDVVPQNATSFLIRDLEVNTQYVFSIMAMNKLGNSKYMPDLLSART----SNKVPPSYSSPSLDEKNLRNFPGFLVIVGTIVGTLLILMNVLLIGCCLHRRSKKRVSEQS-------NQTSKSATIEMYAPSSYNDTVTGETLSSVSEKSETYSN 1122          

HSP 2 Score: 124.02 bits (310), Expect = 7.137e-25
Identity = 177/729 (24.28%), Postives = 280/729 (38.41%), Query Frame = 0
Query:   14 GNPLAEVVWYRGDEQIDFS--------YYTTGRESRNMYTFVAS--SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTI-----EGPTEANVGDTLSFDCITTNSNPPAIVQW----VVDSRSAQENFTVRTESERGG-----WETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI--SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPI-FDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATISDI-TDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAG-EFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASD-KRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV 704
             N   +V W +    + FS        Y   G     +Y    S  S +++  Y+C+       + + A+  L+V   P  V I         E         +C   N+ P A + W    V  +   +E+  +    + G      ++T S IS+     D     +C A +  L   +    T+  SV YPPG P I G   G  +  GQ+  L C S  GNP A L WY+  E I+   +         Y       TA+ SDN   Y+CEASN        PL + + ++V F P  + + S P   + G    L C T +SNP A + W    G  + N     V S P GG +TT+ +   VE     +  IC   N +L E+V    T+ V       P   FI         P  T+  +          S   NP   T  W    N+   N  L + +      I  L N+T    ++  RY+ +  NS  +  L   I ++V +      I  + + +   E A   C   +    A  +SW R G  ++G     +  +  GT S      N++++  G  + C A N         +  L V  +P  ++   N +A +  + +  ++T +A G P ++ ++W++ G  I Q       E I  +                 L+I  +S  D G+Y C A N +G S   V + V
Sbjct:   48 ANLAGQVQWTKDGFALGFSSVIPGFPRYSVIGDRRHGIYNLRVSNASLEDDAEYQCQVGPARLHKAIRANARLNVISPPSSVEILDHRHNSKIEIKENQEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQIRMAYRTAGRVSENVYT-----FTADASDNKARYKCEASN---IMSKAPLKAEVDMTVLFSPAHVTI-SGPTEARVGDPVPLTCTTANSNPPAEIKWMV-AGRQVRNATSRTVVS-PEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINV----LYPPSAPFIHGYTQGSYIPAGTVQKIS-------CTSSGGNPL-ATLTWYK--NDKKINSVLKTTDKSVTAEITILTNVT----DNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRKEPLELKPNEQATLTCDSSSSNPPAK-LSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKD--NEEAVTGIEGEPLIITLKADGNPQNIAYTWTKDGLPIIQSSSSSGIERIISDG--------------PVLNITRLSRHDAGTYSCEALNSQGSSVAQVNITV 730          

HSP 3 Score: 92.8189 bits (229), Expect = 2.710e-15
Identity = 170/755 (22.52%), Postives = 288/755 (38.15%), Query Frame = 0
Query:    3 QTVSLICVSHGGNPLAEVVWYRGDEQIDF---------------SYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVE--PLTADIVLSVQFAPDKVTIEGPTEANV---GDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPE---VPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIY-----SIQNLLNITEARKEDAGRYKFKVNNSEGKSRL--KIKIDVLYGATI-SDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSM-----HLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG-ISRYAVYLNVTSRPDT---PTLL 714
            Q   L C      P A++VWYRG+ +++                    T  ++ +  +   ++ED+   Y C+A++       PL   + LSV + P    IEG TE      G T+   C +   NPPA + W  +         +R      G  + +  +     +D      C A N      +     ++VL+ P   +I+G     +   G    LTC +   NP A + W   G ++   T++     E      S +      +   +V  C   N    Q +  + ST T++V + P    +    +   +P AG    + C +   NP A + W +ND   I +  +   +S      + TNV   D EAR     + CEA N      + + ITM V    +  P    I K   ++ P        E + L  + S    P  +++     + E IP   L +   P ++     SI+  LNITE  + +   Y  +  N   +  +   I + VLY      D  +AV  + GE        D  P      +W++ G  +  ++S  +G   +      L +  +S+ DAG + C A N  +         + V+    I  +  N     +  + A L+C A G P  D   +W R              ++  D  ++ + +V+      S L I +V+  D G++ CI  N  G ++   V L V  +P+    P LL
Sbjct:  146 QEFHLECRVRNAKPAAKIVWYRGNVELNIPDREDQIIEVPGKNGDKRVTRYDTHSRISLKPTAEDDYAEYTCEARHEALSPDIPLRTTVQLSVFYPPGPPYIEGYTEGETIRRGQTVELVCRSRGGNPPAQLIWYKNGEQ------IRMAYRTAGRVSENVYTFTADASDNKARYKCEASNIMSKAPLKAEVDMTVLFSPAHVTISGPTEARV---GDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLN---MQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIP-AGTVQKISCTSSGGNPLATLTWYKNDK-KINSVLKTTDKSVTAEITILTNV--TDNEAR-----YRCEAANSATEIPLFETITMNV----YFPPDHVKIRKEPLELKP-------NEQATLTCDSSSSNPPAKLSW-----WREGIPVQGLLNTTKPGLHGGTVSSIELKLNITE--QLNGIVYTCQATNEALQRSVHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNI-AYTWTKDGLPII-QSSSSSGIERIISDGPVLNITRLSRHDAGTYSCEALN--SQGSSVAQVNITVQYAATIVATSEN--VVVNPNEDATLSCTADGNPLSDDTITWKR--------------DDFPD--FDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALL 839          

HSP 4 Score: 88.9669 bits (219), Expect = 4.234e-14
Identity = 142/614 (23.13%), Postives = 238/614 (38.76%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTT------GRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQG---TTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSI----ENQGEIRVRS-GPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQN---LLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVV---TGETAHFECVVDAKPITADTISWSRVGFDMEGRTS---------MQNGTNSMHLTVYNVSKEDAGEFLCTANNGIA 581
            +G  V L C +   NP AE+ W     Q+  +   T      G  + +  T V      +    C   N    E + +   ++V + P    I G T+ +    G      C ++  NP A + W  + +    N  ++T  +       + I++L    D      C A N+     + +T T++V +PP    I   +P  +    Q+  LTC S + NP A L+W+R    +QG   TT+      T +  + +L++T     NG+VY C+A+N A  +    +   ITL V +KP+      +      G+   +  +   +       W + DGL I     + G  R+ S GP        VL I   +R    T+ CEA N + G SV   + +TV+  +               I  T     V    D  ++ +   NP  D T  W     ++ P    D D    +   +N    L IT+  ++D G ++  VNN  G    K  + ++      D   A++      G+T    C   A P+      W+R G  +   T+         +   T+   L + +V+  D G + C A N + 
Sbjct:  352 VGDPVPLTCTTANSNPPAEIKWMVAGRQVRNATSRTVVSPEGGWITTSNITAVVEPNRRSLVVICHGLNMQLTENVVSTHTINVLYPPSAPFIHGYTQGSYIPAGTVQKISCTSSGGNPLATLTWYKNDKKI--NSVLKTTDK----SVTAEITILTNVTDNEARYRCEAANSATEIPLFETITMNVYFPPDHVKIRK-EPLELKPNEQA-TLTCDSSSSNPPAKLSWWREGIPVQGLLNTTKPGLHGGTVSSIELKLNITEQL--NGIVYTCQATNEALQRS---VHDAITLQVLYKPVFDKDNEEAVTGIEGEPLIITLKADGNPQNIAYTWTK-DGLPIIQSSSSSGIERIISDGP--------VLNITRLSRHDAGTYSCEALNSQ-GSSVAQ-VNITVQYAA--------------TIVATSENVVVNPNEDATLSCTADGNPLSDDTITWK---RDDFP----DFDARTSVMYDKNGTSYLRITDVTRDDLGNFQCIVNNGVGNVTTKEVMLIVKHKPEIDQHPALLKFASDAGDTGRVVCRSQASPLAK--YKWARSGAPITANTTGKYYTTYRQIDALTSESVLFITHVTSSDYGNYECVARNELG 918          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold14_size734282-snap-gene-4.11 (protein:Tk11303 transcript:maker-scaffold14_size734282-snap-gene-4.11-mRNA-1 annotation:"nephrin ")

HSP 1 Score: 809.29 bits (2089), Expect = 0.000e+0
Identity = 411/816 (50.37%), Postives = 554/816 (67.89%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLY----------------PPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTD----TIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIP--RIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSE 794
            +GQTV+L CVSHGGNPLAE+VWY+  E IDFS+ ++GRESRN +TF+A+++DNN  YRC+AKN +S EP+ A+I L+VQFAPDKVTI GPTEA  GD L+F+C T NSNPPA +QW+VD     ENFT +  S  GGW ++S++S  +GP DRN +ISCYA+N  LGET+V+TH +SVL                 PP +P+ITG+  G +++EGQ RRLTCISMAGNPLA LTW+RG++ I+GTT    +     Y+ SEL + AN +DN   YRCEA+NPAT +   P  +++ L+V FKP  + +  DP  PKAGKKA L CE+ SSNPTA+++WR N G  ++   +  V++G +GGN+T+N+L IDV     G  FICEA N  L  SV+D  T++VK             KP F  D  D     +F+VEEGS L+++++ +ANP ++ Y+W +P  +NIP++   +  +P  R+      LN+T A++ DAG+YK K +NSEGK+  K K+DVLY  +I++ITD+V+V  GE A F C VDA PI  DTI W R  FDM  R +  N +N M+LTV NV++ D+G+F C ANNGI +     S FLLV+ +P I+ESP  +KAASDK  T VLTCRA G P V F+WSR GSVI             ++K+E     ID++T+ES L +KNV+S DYG+YDCIAQNEEG+ R+ +YLNVTSRPD P  L V N TY SV L W PGFDGG +QSF IR+RK+ S+ Y YVDV+    ++FEV+DLK+GT Y F++ + N +G+S++T++
Sbjct:  174 LGQTVTLTCVSHGGNPLAEIVWYKNGEGIDFSFTSSGRESRNTFTFMAATDDNNANYRCEAKNFLSPEPMVAEISLAVQFAPDKVTITGPTEAKAGDELNFECETGNSNPPASIQWLVDGVQVHENFTHKATSPNGGWVSKSDLSTKVGPQDRNIMISCYAVNQALGETIVETHMVSVLCEYIFAYVFGSILAFSDPPSQPNITGLVYGDVLEEGQMRRLTCISMAGNPLADLTWFRGEKKIEGTTT--IKGGDGKYSSSELTVIANRTDNDAPYRCEAANPATPE---PKKTSVKLAVLFKPALVKITVDPSDPKAGKKAILTCESSSSNPTADIVWRYN-GKRMKGSDQ-AVKAGEYGGNVTSNLLEIDVTPEHQGAVFICEAKNPVLETSVHDAKTLSVKY------------KPQFPDDTPDDDSIQVFTVEEGSSLLIDVTPKANPSEIEYKWTSPDRDNIPDL---AKALPESRLIVDGGKLNVTFAKRMDAGKYKVKASNSEGKTTFKFKLDVLYEPSITEITDSVIVKPGENAEFSCFVDANPIQVDTIQWKREDFDMT-RVTTTNISNVMYLTVKNVTESDSGKFECVANNGIGEEVKNVS-FLLVKHKPAIDESPGIAKAASDKGLTGVLTCRATGVPKVRFTWSREGSVIDPS---------TNDKFETDEIQIDKLTFESFLSVKNVTSSDYGAYDCIAQNEEGLDRHTIYLNVTSRPDAPAYLQVLNVTYTSVTLKWTPGFDGGFEQSFRIRFRKEDSQVYHYVDVYPPGVDTFEVQDLKVGTTYRFAIMSHNYIGESDYTTD 956          

HSP 2 Score: 151.369 bits (381), Expect = 2.532e-37
Identity = 173/690 (25.07%), Postives = 287/690 (41.59%), Query Frame = 0
Query:   50 SEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDT-----LSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTE---SERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI-SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSF----LNPLIDFIDKPIFDIDPTDTIFS--VEEGS-DLVVNISVRANPF-DVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNN--SEGKSRLKIKIDVLYGATISDIT-DAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEG-----RTSMQNGTNSMHLTVYNVSKEDAGE-FLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAAS----DKRKTAVLTCRAQGTP-DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            S D++  Y C+        P+ A   L V   P  + IEG T     D          CI  ++ P A + W  +S       T  TE   ++ G    +S I     P D      C A++  LG T ++   + SV +PPG P I G   G  +  GQ+  LTC+S  GNPLA + WY+  E I     D   T +   +++     A   DN   YRCEA N  + +   P+ + I+L+V+F P  + + + P   KAG +    CETG+SNP A++ W   DG+ +      +  S P GG ++ + L   V  +D  +   C A N+ LGE++ +   ++V C+         ++ F D P    + T  ++   +EEG    +  IS+  NP  D+T+       E    I          YS   L  I      DA  Y+ +  N  +    +  +K+ VL+   +  IT D      G+ A   C   +   TAD I W   G  M+G     +     G  + +L   +V+ E  G  F+C A N + +     +  L V+ +P+  +   +  +      ++  + ++    +  P ++ + W+         P+ D   ++     E +  ++D      +L++      D G Y   A N EG + +   L+V   P
Sbjct:   17 SLDDDADYECQVGPANFNAPIRASAHLHVLLPPTSIKIEGYTPGQRVDIKENEECELTCIVADAKPKASIIWFRNSLEFTPESTEDTEEDGTKEGRKTVKSQIKFHPRPRDNGAKYFCQAVHPALGGTPMKVGIVLSVQFPPGPPEIQGYLQGETVRLGQTVTLTCVSHGGNPLAEIVWYKNGEGI-----DFSFTSSGRESRNTFTFMAATDDNNANYRCEAKNFLSPE---PMVAEISLAVQFAPDKVTI-TGPTEAKAGDELNFECETGNSNPPASIQWLV-DGVQVHENFTHKATS-PNGGWVSKSDLSTKVGPQDRNIMISCYAVNQALGETIVETHMVSVLCEYIFAYVFGSILAFSDPPS-QPNITGLVYGDVLEEGQMRRLTCISMAGNPLADLTWFRGEKKIEGTTTIKGGDGK----YSSSELTVIANRTDNDA-PYRCEAANPATPEPKKTSVKLAVLFKPALVKITVDPSDPKAGKKAILTCESSSSNPTAD-IVWRYNGKRMKGSDQAVKAGEYGGNVTSNLLEIDVTPEHQGAVFICEAKNPVLETSVHDAKTLSVKYKPQFPDDTPDDDSIQVFTVEEGSSLLIDVTPKANPSEIEYKWT--------SPDRDNIPDLAKALPESRL-IVDG----GKLNVTFAKRMDAGKYKVKASNSEGKTTFKFKLDVLYEP 675          

HSP 3 Score: 92.0485 bits (227), Expect = 5.864e-19
Identity = 96/417 (23.02%), Postives = 170/417 (40.77%), Query Frame = 0
Query:    7 LICVSHGGNPLAEVVWYRGDEQIDFSYYTT----------GRES-RNMYTFVASSEDNNKRYRCKAKN-TVSVEPLTADIVLSVQFAPDKVTIEGPTEAN---VGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWY-RGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQ---ESGPLSSTITLSVRFKPITIAVKSDP------------EVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFL 392
            L C+     P A ++W+R    ++F+  +T          GR++ ++   F     DN  +Y C+A +  +   P+   IVLSVQF P    I+G  +     +G T++  C++   NP A + W  +      +FT        G E+R+  + +   +D N    C A N    E +V   +++V + P K +ITG    T    G      C +   NP A++ W   G ++ +  T           ++S+L       D  ++  C A N A  +   E+  +S        +   +I   SDP            +V + G+   L C + + NP A++ W R +    + +G   ++ G   G  +++ L +     D+   + CEA N    E     + + V  K  L
Sbjct:   73 LTCIVADAKPKASIIWFRN--SLEFTPESTEDTEEDGTKEGRKTVKSQIKFHPRPRDNGAKYFCQAVHPALGGTPMKVGIVLSVQFPPGPPEIQGYLQGETVRLGQTVTLTCVSHGGNPLAEIVWYKNGEGIDFSFT------SSGRESRNTFTFMAATDDNNANYRCEAKNFLSPEPMVAEISLAVQFAPDKVTITG---PTEAKAGDELNFECETGNSNPPASIQWLVDGVQVHENFTHKATSPNGGWVSKSDLSTKVGPQDRNIMISCYAVNQALGETIVETHMVSVLCEYIFAYVFGSILAFSDPPSQPNITGLVYGDVLEEGQMRRLTCISMAGNPLADLTWFRGEK---KIEGTTTIKGG--DGKYSSSELTVIANRTDNDAPYRCEAANPATPEPKKTSVKLAVLFKPAL 473          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold542_size141805-snap-gene-0.10 (protein:Tk09917 transcript:maker-scaffold542_size141805-snap-gene-0.10-mRNA-1 annotation:"sticks and isoform b")

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 332/827 (40.15%), Postives = 485/827 (58.65%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVV--DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNS--------------MHLTVYNVSKEDAGEFLCTANNGIADMKD----TKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGS--VISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSK-TYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYL 804
            +GQ V+L C S GGNPLA+V WYR D  +D SY T+G ESRN Y+F+AS+EDNN  +RC+A N ++ +P+ A+IVL+VQFAPD + ++GPTEA +G+ ++++C T NSNPPA +QWVV   SRSA   +TV   S  GGW T SNI+V + P DR+K+I C  +N++L +   ++  +SV+YPPG P I G+    +M  G  +R+TC S+AGNPLATL W+  +  ++      D     NYA +EL      +DNG   RC+ASN A  +   PL ++  L+V+F    + +  DPE P+AG    L CET +S P + + W  N G  +E+  ++ +  G FGG +TT+ L + + A+  G    CEA+N  +   V+D IT++V  K        F  KP+F    T+  F V EG    VN++  ANP  VTY WA    E+  +  + S    R+ +    L I   ++ DAG Y     N +G  + KI+I VLY   I D+T+ V+V  GE AH EC VDA P+   TI W R  +DM  RT    G+ +              + LTV N + ED+G F C A+NGI +  +       + LLVR +PRI E   +S+AA+D  +T    C A+G P+V FSW R G   V+   P   E      +K+ +    +D +TW SEL I NV++ DYG+Y+C+A+N EG+SR+ + L+V SRPD P+ L + N T++SV+L W PGF GG +Q F I+Y+ + S    ++ DV+ ++T+  ++  L+ G +Y+ +V AFN+LG+S +T E V V T + L
Sbjct:  177 LGQAVTLTCTSAGGNPLAKVAWYRNDALVDTSYTTSGLESRNTYSFLASTEDNNALFRCEAHNELNAQPMEAEIVLAVQFAPDSLDLKGPTEAKIGEIITYECSTANSNPPASIQWVVGNQSRSADHTYTV--TSPMGGWITHSNITVSLTPKDRSKMIICNGLNSELNDAKSESRMLSVIYPPGSPQIQGLSDDEVMSAGVLKRVTCTSLAGNPLATLKWFLDERPLESFYSTKD-----NYASAELEFVPRNADNGATLRCQASNTAVLE---PLEASQRLAVQFPSKFVKIALDPEQPQAGMNVTLSCETDTSYPASELAWWHN-GERLESTTDM-ILDGDFGGYITTSYLQLSLTAQHDGAVVTCEASNPAVQNRVHDAITLSVARKY----RSKFSYKPVFVRKSTEP-FQVIEGRAATVNMTAGANPTPVTYLWAKDNAEDGTDTEILSS---RVTAQNGALQIDRVQRSDAGYYLVTATNKQGSVQTKIEIQVLYPPRIVDVTNQVLVGPGEPAHLECAVDAMPLGPQTIRWERANYDMASRTKTTTGSPASLSAGKSASSNVGVVLLTVLNATAEDSGMFWCVADNGIDNDGEFPQVRNGSLLLVRHKPRIREDALHSRAAADLGETGHFICEARGVPNVTFSWMRAGGAYVVPSNPRAPE------KKFTMTATRVDPLTWRSELFIFNVTNGDYGAYECVARNTEGVSRHRMPLDVKSRPDPPSALQIVNVTHDSVHLVWTPGFHGGMEQYFRIKYQAENSPGDTRFHDVYPANTHDLQLTGLRAGQRYTLAVMAFNNLGESNYTVEPVVVRTATAL 977          

HSP 2 Score: 115.931 bits (289), Expect = 3.181e-26
Identity = 93/355 (26.20%), Postives = 159/355 (44.79%), Query Frame = 0
Query:   46 FVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEG-----PTEANVGDTLSFDCITTNSNPPAIVQW-------VVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTI-SVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIW-RRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTV 386
             V +S +++  Y C+        P+ A   L+V   P ++ I+G       E    + +   C   ++ P A + W         D R  +E  +     E G   + S I       D     +C A ++ +    +++  + SVL+PPG P I+G   G     GQ+  LTC S  GNPLA + WYR D ++     D   T +   +++     A+  DN  ++RCEA N   AQ   P+ + I L+V+F P ++ +K  P   K G+     C T +SNP A++ W   N   S ++   +   + P GG +T + + + +  +D     IC   N EL ++ ++   ++V
Sbjct:   13 IVNASINDDAIYECQVGPAPGNRPIRATARLTVLLPPTRIEIDGVFSGTRLEVKENEEVELRCTVFDARPKANIIWYRQNSVFTTDLREDEERPSALGRDE-GRITSTSTIRFRPSSADNGITWACEAQHSAITYAPLRSSVLLSVLHPPGPPEISGYIEGETKRLGQAVTLTCTSAGGNPLAKVAWYRNDALV-----DTSYTTSGLESRNTYSFLASTEDNNALFRCEAHNELNAQ---PMEAEIVLAVQFAPDSLDLKG-PTEAKIGEIITYECSTANSNPPASIQWVVGNQSRSADHTYTV---TSPMGGWITHSNITVSLTPKDRSKMIICNGLNSELNDAKSESRMLSV 354          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold61_size441589-processed-gene-2.1 (protein:Tk06307 transcript:snap_masked-scaffold61_size441589-processed-gene-2.1-mRNA-1 annotation:"protein turtle isoform x8")

HSP 1 Score: 119.783 bits (299), Expect = 2.767e-27
Identity = 132/528 (25.00%), Postives = 228/528 (43.18%), Query Frame = 0
Query:  307 LFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG--ISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDG--GHKQSFIIRYRKKGSKTYQYVDVHHSSTNS--FEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQG 822
            L C+ G + P   ++W + D        +I  R G F  N  T +   ++  RD G  + C A N       N  I  T K        +      +    P +   +V EG  +  +    ANP +++  W   + + +P I    D    I    NL+ ITEA+ +  GRY  KV N  G+       + + Y A ++   D   +  G      C ++A P     ++W++       F + G   ++NG+    + +  VS+E AG++ CT  N   +   +K+  ++++  P I   P      S   K A + CRA GTP    SW R+           +   +  +++++ YN         EL I  +   D+G Y+C+ +NE    ++R  + +  T+ P  PT L V N    +V + W+PG+ G    +Q++ IRYR+K SK   ++++  S  ++   ++ +L   TKY F V   N+ GD  + SE +  +T     G   P  +++  T   G
Sbjct:  627 LNCQAGGT-PAPEILWFKGD-----KAIQISHRIGVF--NDGTELRLSNILERDLG-DYTCIARNG----VYNGSIHFTTK--------LVMAGAAVITNQPQNQ--TVAEGRPVEFHCKGEANPGNISVSW---FKDGMP-IRSMGDGGFAIRDDGNLV-ITEAKSKAQGRYTCKVTNGIGRPIEATAFLTIEYPARVTYHPDVQYIPRGLQGIIHCHIEANP-PIQFVTWTKDKRIFDPFGIPGIMGIENGS----ILIDRVSQEHAGDYECTPFNIHGNDGSSKTMQVIIKDPPTILRRPEQEYHRSVGGKVA-MPCRAIGTPKPDISWRRV-----------DGHHLPRKRHKV-YN--------GELTIDALHKSDHGVYECVVENEVATIVARTELKIERTT-PHAPTNLTVANSQTFAVTIEWMPGYSGCAACQQTYKIRYREKESKFPNWIELPVSPADARRIQIYNLSSNTKYEFQVIGTNEYGDGMY-SEIIEAQTKDIPPGEAPPAAQNLSLTQEAG 1098          

HSP 2 Score: 74.7146 bits (182), Expect = 1.388e-13
Identity = 99/414 (23.91%), Postives = 171/414 (41.30%), Query Frame = 0
Query:  307 LFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVG-----FDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEG--ISRYAVYLNVTSRPDTPT 712
            L C+ G + P   ++W + D        +I  R G F  N  T +   ++  RD G  + C A N       N  I  T K        +      +    P +   +V EG  +  +    ANP +++  W   + + +P I    D    I    NL+ ITEA+ +  GRY  KV N  G+       + + Y A ++   D   +  G      C ++A P     ++W++       F + G   ++NG+    + +  VS+E AG++ CT  N   +   +K+  ++++  P I   P      S   K A + CRA GTP    SW R+           +   +  +++++ YN         EL I  +   D+G Y+C+ +NE    ++R  + +  T+ P  PT
Sbjct:  183 LNCQAGGT-PAPEILWFKGD-----KAIQISHRIGVF--NDGTELRLSNILERDLG-DYTCIARNG----VYNGSIHFTTK--------LVMAGAAVITNQPQNQ--TVAEGRPVEFHCKGEANPGNISVSW---FKDGMP-IRSMGDGGFAIRDDGNLV-ITEAKSKAQGRYTCKVTNGIGRPIEATAFLTIEYPARVTYHPDVQYIPRGLQGIIHCHIEANP-PIQFVTWTKDKRIFDPFGIPGIMGIENGS----ILIDRVSQEHAGDYECTPFNIHGNDGSSKTMQVIIKDPPTILRRPEQEYHRSVGGKVA-MPCRAIGTPKPDISWRRV-----------DGHHLPRKRHKV-YN--------GELTIDALHKSDHGVYECVVENEVATIVARTELKIERTT-PHAPT 541          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold44_size478958-snap-gene-1.11 (protein:Tk06921 transcript:maker-scaffold44_size478958-snap-gene-1.11-mRNA-1 annotation:"PREDICTED: uncharacterized protein LOC100881893")

HSP 1 Score: 108.612 bits (270), Expect = 3.362e-24
Identity = 113/460 (24.57%), Postives = 196/460 (42.61%), Query Frame = 0
Query:  176 ISVLYPPGKPSI---TGIDPGTIMD---EGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHL-TANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK-IKIDVLYGATISDITDAVMVVTGET-AHFECVVDAKPITADTISWSRVGFDMEGRTSMQNG----TNSMH--LTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTC 620
            ++V+ PP  P+I   +G +  T++    EG S  L CI+  GNP   +TW+R   ++  T     ET      Q+ L +      D G +  C+ASN      S P+S+ IT+ ++F P  + + +  E   AGK   L CE+G S P+A V W +N+    +   +I        GNLT + L       DHG T  C A N ++   V+  +    K   F  P++        + D      ++ EG+D+    ++ ANP      W   +N+    + +  D    +    + L +    +  +G Y    +N EG ++   I++ ++Y     +    +  V+ +  +   C + A P     I WS   F+    T M N     +NS H  L+       D G   C+A+N + +M D    F ++   P   ++P+N    +   ++  + C
Sbjct:   67 VTVVVPPMIPTIFDESGQEAQTVVGPFLEGDSVVLQCIASGGNPAPQVTWWRDSHLLDSTF----ETTFSQTVQNTLTIDKVTRQDLGAILTCQASN---NNISIPVSNRITIDLKFPPSGVKILTKKEPLSAGKYYRLECESGGSRPSAIVTWWKNNKFMGKTPSKIVDN-----GNLTISTLEFKPRPSDHGKTLTCRAENLDI---VSSALEEQWKLVIFHAPIVSLTFGANLNPD------NIAEGNDVYFECTISANPEVYKVIWL--HND----VMVSHDQSHGVIISGSSLVLQGVHRRQSGMYACVASNLEGDTQSNTIQLKIMYKPLCGNFKRIIQGVSRDRDSQIICPILAHPQPTRFI-WS---FNNSLETKMINASQFKSNSTHSELSYQPKDDNDFGLLFCSASNALGEMAD-PCVFKIIPAGP--PDAPSNCSVVNQTTESLEVEC 492          

HSP 2 Score: 83.5741 bits (205), Expect = 2.120e-16
Identity = 94/384 (24.48%), Postives = 160/384 (41.67%), Query Frame = 0
Query:    2 GQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRES-RNMYTF-VASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA-NVGDTLSFDCITTNSNPPAIVQWVVDSRSAQENFTVRTESE--RGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPPGKPSIT---GIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGID-VEARDHGVTFICEATNEELGE 376
            G +V L C++ GGNP  +V W+R    +D ++ TT  ++ +N  T    + +D      C+A N     P++  I + ++F P  V I    E  + G     +C +  S P AIV W  +++     F  +T S+    G  T S +     P+D  K ++C A N D+  + ++     V++     S+T    ++P  I  EG      C   A   +  + W   D ++       D++     + S L L   +     +Y C ASN     E    S+TI L + +KP+    K   +     + + + C   +       IW  N+ L  +      + +  F  N T + L     +  D G+ F C A+N  LGE
Sbjct:   96 GDSVVLQCIASGGNPAPQVTWWRDSHLLDSTFETTFSQTVQNTLTIDKVTRQDLGAILTCQASNNNISIPVSNRITIDLKFPPSGVKILTKKEPLSAGKYYRLECESGGSRPSAIVTWWKNNK-----FMGKTPSKIVDNGNLTISTLEFKPRPSDHGKTLTCRAENLDIVSSALEEQWKLVIFHAPIVSLTFGANLNPDNIA-EGNDVYFECTISANPEVYKVIWLHNDVMV-----SHDQSHGVIISGSSLVLQGVHRRQSGMYACVASN----LEGDTQSNTIQLKIMYKPLCGNFKRIIQGVSRDRDSQIICPILAHPQPTRFIWSFNNSLETK-----MINASQFKSNSTHSELSYQPKDDNDFGLLF-CSASN-ALGE 457          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold68_size422247-snap-gene-2.11 (protein:Tk11392 transcript:maker-scaffold68_size422247-snap-gene-2.11-mRNA-1 annotation:"nephrin- partial")

HSP 1 Score: 98.9821 bits (245), Expect = 3.449e-21
Identity = 67/247 (27.13%), Postives = 119/247 (48.18%), Query Frame = 0
Query:   53 NNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVD------SRSAQENFTVRTESERG----GWETRSNISVLIGPNDR----NKIISCYAINNDLGETVVQ-THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISM-AGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSV 283
            N  +  C  +N VS   +   + ++ +F P++V++ GP +    +++ F C ++ + P A + W V+      ++ + EN   R  +E          +++  +L  P       +  + C+A +  LGE ++   H + VL+PPG PSI+G+  G  ++ G  R LTC S  AG+P A + WY G ++++   Q D +T     A + +        NG   RCEA N A      P++ TI L +
Sbjct:  349 NGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDT-----ALAHITFVPQLEYNGSELRCEAMNEAIGV---PVTQTIRLDL 587          

HSP 2 Score: 94.7449 bits (234), Expect = 7.551e-20
Identity = 73/251 (29.08%), Postives = 115/251 (45.82%), Query Frame = 0
Query:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQW------------------------VVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-----ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW 219
            +G  V+LIC +  G+P  E+ +YR  EQ+      T R+ RN +TF AS  DNN  + C A N  S+    +D+ L+V+F P  V I+GP      N+ + ++  C +  SNP   + +                        +V S+S++  ++V    E+GGW T +NI  L+      K+     I C   N      ++ + +I+  +PP + S+TG  P  I    +S   TC S    P A L W
Sbjct:  177 VGHMVTLICKAQSGHPEPELAFYRNSEQMG-----TARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMS-RSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSV----EQGGWITSANI--LLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG--PAKI-KASESVVFTCQSSPAQPAARLAW 412          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold93_size381549-snap-gene-2.25 (protein:Tk04402 transcript:maker-scaffold93_size381549-snap-gene-2.25-mRNA-1 annotation:"hypothetical protein L798_13523 partial")

HSP 1 Score: 84.3445 bits (207), Expect = 5.619e-17
Identity = 84/341 (24.63%), Postives = 143/341 (41.94%), Query Frame = 0
Query:  516 GETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTP--DVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISR--YAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSE------FTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVILISCYV 846
            G+  +FEC V A P     ISW   G ++      +   ++  L +  V++ D+G + CTA+N   D  ++    L +R +P  +         + + K  V +C     P  D+ F W    S                E+ +++ N+++     S  H    +  DYGS  C A N  G        +L    +PD      V N TY+++++T I GFDGG  Q F++      S  +   +  +    +F V  L  GT Y  SV ++N  G SE      FT++  A  + S          K  E ++++    T+T +  +LLI+   L+  ++
Sbjct:  153 GDDVYFECAVKASP-RPYKISWKHNGKELAHDIQKKVIISNQSLVLQKVTRSDSGVYTCTAHNAEGD-GESNPLSLDIRYKPFCKPDQVQVYGVAKREKIRV-SCDVISNPSVDLKFEWVFNSS---------------SERLDLQENLVEVRGTRSLAHHIPQTEMDYGSLMCWATNSIGRQEDPCIFHLIPAGKPDPVQNCTVKNQTYSTLHITCIKGFDGGLPQGFVMEVMDAKSH-FIVANTTNLRDPAFTVTGLHSGTGYIVSVNSYNSKGHSEPMRIHAFTTKAAAEASAS------DQTPKTGESSLTRMGQFTVTPILAILLIVGGALVLVFL 468          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold204_size260821-processed-gene-1.10 (protein:Tk07812 transcript:snap_masked-scaffold204_size260821-processed-gene-1.10-mRNA-1 annotation:"neural cell adhesion molecule 2-like")

HSP 1 Score: 80.8777 bits (198), Expect = 1.437e-15
Identity = 101/427 (23.65%), Postives = 182/427 (42.62%), Query Frame = 0
Query:  169 TVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRL------TCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTA---NYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDF-IDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLK-IKIDVLYG-ATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDA-GEFLCTANNGIAD 582
            TV+    ++++  P KP I   +   +M      RL       C+   GNPL  ++W+R  ++     Q+ D +  ++  Q+ L ++A   +Y D+   + C A N    +   P +ST+T+++    + I++ + P+   A  K  + C+     PT  + WR +D +   N+    + +     NLTT  L    + +D G    C A N  +  + N  +T+ V    +  P++   I+  I  +DP +    + EG  L +  +++A+P+     W     E      L   N  R+   +  L +  A K  +G+Y    +N EG    K + I V Y    I+   +       +    +C VDAKP  + T  W  +    E R  + +  + M    Y  +  D  GE LC A+N + +
Sbjct:  132 TVITQVKLNIVEEPKKPVILDAEGSAVMGNIGPFRLRKPLILVCLVEGGNPLPEVSWWRDGQMYD---QEGDPSTYEDVLQNTLVISALDRSYHDSH--FECRAINNNVTE---PPTSTVTITLELPILDISLSNLPDPVTADVKYEVLCQVIGPQPTPTITWRLSDVILPANEPRYSLEN-----NLTTTELHFMPKIKDQGKILSCTADN-GIFPAQNKSMTLNV----YFIPVVHVEIENDI--VDPAN----IREGDTLELKCNIQAHPWVWRILWYKDGEE------LVGSN--RVLIDEQRLKLQNATKALSGQYVCSASNVEGDGFSKPMMITVNYKPVCIAPTIEYKGDNRSQGIDLKCQVDAKP-KSSTFRW--LFNSSETRFEIPSAESIMSFGNYKPTSGDQHGEVLCWASNDLGE 523          

HSP 2 Score: 77.0258 bits (188), Expect = 2.201e-14
Identity = 137/637 (21.51%), Postives = 245/637 (38.46%), Query Frame = 0
Query:  244 SELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSV---RFKPITIAVKSDPEVPKAG-----KKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARD-HGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWAN---PYNENIPNITLDSDNIPRIYSIQNLLNITE----ARKEDAGRYK--------FKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDV-VFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVT--SRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQSFIIR-YRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEGMVKPEDKDVERTISQGIILTITLVSGLLLILNVIL---ISCYVKRR 849
            ++L +++   D+  +Y+C        +ES  + + + L++     KP+ +  +    +   G     K   L C     NP   V W R DG   + +G+          ++  N L I    R  H   F C A N  + E     +T+T++      P++D     + D    D  + V     L   I  +  P  +T+R ++   P NE            PR YS++N L  TE     + +D G+          F   N      +   I V++    +DI D   +  G+T   +C + A P     I W + G ++ G   +    +   L + N +K  +G+++C+A+N   D   +K   + V  +P +  +P       ++ +   L C+    P    F W    S                   E ++ +    +  S  + K  S   +G   C A N+ G        +V     P  P    + N+T +++ +T  PGF GG  Q F++  +    + +   V  + +   +  V  L   + Y  SVKA N+ G+S        V      EG ++   + VE +    ++  I  + G ++IL   +   I+C  KRR
Sbjct:  106 AQLIISSVEMDDMGIYKCRVD----FRESPTVITQVKLNIVEEPKKPVILDAEGSAVMGNIGPFRLRKPLILVCLVEGGNPLPEVSWWR-DGQMYDQEGDPSTYE-----DVLQNTLVISALDRSYHDSHFECRAINNNVTEPPTSTVTITLEL-----PILDISLSNLPDPVTADVKYEV-----LCQVIGPQPTP-TITWRLSDVILPANE------------PR-YSLENNLTTTELHFMPKIKDQGKILSCTADNGIFPAQNKSMTLNVYF-IPVVHVEIENDIVDPANIREGDTLELKCNIQAHPWVW-RILWYKDGEELVGSNRVL--IDEQRLKLQNATKALSGQYVCSASNVEGD-GFSKPMMITVNYKP-VCIAPTIEYKGDNRSQGIDLKCQVDAKPKSSTFRWLFNSS-------------------ETRFEIPSAESIMSFGNYKPTSGDQHGEVLCWASNDLGEQLKPCVFHVVPLGAPQAPRDCQISNHTASTIEVTCTPGFSGGLVQHFVMEVFETMPNNSQVLVASNWTEDATISVWGLVPESNYIISVKAVNEKGESS------PVYVGGKTEGFIQSLPRAVEES-RLPLLFVIIGILGTMMILGACVTATIACKRKRR 676          

HSP 3 Score: 62.003 bits (149), Expect = 9.652e-10
Identity = 71/308 (23.05%), Postives = 129/308 (41.88%), Query Frame = 0
Query:    7 LICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSED---NNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFD------CITTNSNPPAIVQWVV--------DSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKIISCYAINNDLGETVVQTHTISVLYPP---GKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQ---DDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIA 291
            L+C+  GGNPL EV W+R  +  D     +  E     T V S+ D   ++  + C+A N    EP T+ + ++++     +++     +N+ D ++ D      C      P   + W +        + R + EN    TE           +  +    D+ KI+SC A +N +     ++ T++V + P    +     +DP  I  EG +  L C   A   +  + WY+  E + G+ +   D+   + +N  ++   L+  Y        C ASN     E    S  + ++V +KP+ IA
Sbjct:  173 LVCLVEGGNPLPEVSWWRDGQMYDQEGDPSTYEDVLQNTLVISALDRSYHDSHFECRAINNNVTEPPTSTVTITLELPILDISL-----SNLPDPVTADVKYEVLCQVIGPQPTPTITWRLSDVILPANEPRYSLENNLTTTE-----------LHFMPKIKDQGKILSCTA-DNGIFPAQNKSMTLNVYFIPVVHVEIENDIVDPANIR-EGDTLELKCNIQAHPWVWRILWYKDGEELVGSNRVLIDEQRLKLQNATKA---LSGQYV-------CSASN----VEGDGFSKPMMITVNYKPVCIA 448          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold108_size357748-snap-gene-2.20 (protein:Tk09444 transcript:maker-scaffold108_size357748-snap-gene-2.20-mRNA-1 annotation:"conserved hypothetical protein")

HSP 1 Score: 79.337 bits (194), Expect = 4.351e-15
Identity = 127/539 (23.56%), Postives = 207/539 (38.40%), Query Frame = 0
Query:  302 GKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWANPYNENIPNITLDSDN---IPRIYSI-QNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGT--NSMH------LTVYNVSKEDAGEFLCTANNGIADMKDTKS----TFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNV----------------TSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQ--SFIIRYRKKGSKTYQYVDVHHSSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVETLSYLEG 806
            G  A L C++   NP  +V W +N  +  E + E +  +    G+L       D    D G  ++C+A N  +G   +   T+ V  K            PIF   P D   + + GS++ +   V  +P               P++    +N   +P+   + + +L I +A   D G Y  +  N  GK    + + V    T         V     A FECVVD  P    +I W++ G     +  M +GT    MH      L +  V KED G ++C+A + +A     K+    T +  +  P I   PAN     +    AVL C+A G P    +W +     +Q+P       +E  +  I+ +          L I N+   D   Y C A +E G + ++  L+V                ++ P  PT   + N    SV ++W      G      + + Y     +T   V     ++ ++ V +LK  + Y F V+A N  G S  +  +  V TL    G
Sbjct:   23 GDTAVLKCQSPRGNPKPSVSWLKNGKVLHELEDEPKRVTLTETGSLVIR----DAVKADQG-DYVCQARNM-VGTRNSGPGTLHVNVK------------PIFIQRPEDV--TSKGGSEVTLICLVAGDP--------------EPHLVWRRENDLALPKGSQVDKGILVIPKAEPHDEGMYSCEATNPVGKVSQSVVLTVHAHPTFLVKPKDQRVGMNGIAKFECVVDGNP--PPSIFWTKEG----NQELMFSGTTHGQMHVSQDGTLVIQGVRKEDGGFYVCSALS-VAGSTLAKAYLEVTAIEDQPPPIIAMGPANQTLPVN--TIAVLPCQASGNPKPSINWFK-----NQQP----LYAVESSRIMIEAS--------GTLVIDNLRMEDTALYTCTAGSESGETSWSASLSVEDPKNPNIIFHKTPDPSTFPQPPTKPKIVNRRSTSVTISWRRNHQTGQSPLIGYTVEYFSFEMETGWVVAALRITSETYTVNNLKPDSSYVFLVRAENSHGMSPPSQMSERVRTLRAFAG 501          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold61_size441589-snap-gene-0.14 (protein:Tk06306 transcript:maker-scaffold61_size441589-snap-gene-0.14-mRNA-1 annotation:"down syndrome cell adhesion molecule isoform x1")

HSP 1 Score: 76.2554 bits (186), Expect = 4.430e-14
Identity = 152/704 (21.59%), Postives = 255/704 (36.22%), Query Frame = 0
Query:  159 CYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGT-IMDEGQSRRLTCISMAGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSVRFKPITIAVKSDPEVPKAGKKAYLFCETGSSNPTANVIWRRNDGLSIENQGEIRVRSGPFGGNLTTNVLGIDVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWAN-----PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKS-RLKIKIDVL---------YGATISDITDAVMVVT-------------------------GETAHFECVVDAKPITADTISWSRV---------------GFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLTWIPGFDGGHKQS-FIIRYRK-KGSKTYQYVDVHH----SSTNSFEVEDLKLGTKYSFSVKAFNDLGDSEFTSENVAVET 800
            C   N+  GE+V    T++       P    ++P   ++D G+S    C    GNP+ ++ W +  E +              + ++ L + +   ++  +Y+C   N    QES   +  + L  RF P       D    + G    L C     +P   V W    G   E+ G ++V S         + L I      +G T+ C+A+++ +G  V+    + V    ++  ++                  V  G  + V   V   P   +  W       P+N+     T+  +    I ++Q        RKEDA  Y     N EG S R  + + V+         +G  + +  D   V+                          G  A  +C  D  P     I+W +                 FD     +++    S  L + N+ K + G +LC A+NGI  +        +  Q P   E    ++ A     T VL C A+G   +   W+     +  +   D    I +E  E      D M   S + IK     D   + C+A N  G    ++ L +  +P+TP  L V + T  +V L+W   ++G    + ++I ++  KG+      DV H           V  L+  T Y F V A ND+G S   S+ V +ET
Sbjct:  419 CMVNNSVGGESVETVLTVTA------PLSVEVEPKQQVVDYGRSATFKC-HYDGNPVKSVYWLKNGEPV-------------GHTETTLRINSVKKEDRGMYQCFVRN---DQESAQATGELKLGGRFDPPEFVSAFDAVTTQPGPMVSLKC-VAKGDPAPEVKWFIY-GSEAESDGVLQVGSFNSDNGDVVSYLNISQARTKYGGTYECQASSK-VG-LVSHSAKLNVNGAPYVRKMLPM---------------KVVAGKSMFVTCPVAGYPIS-SVTWEKDGRQLPFNDRQ---TVYRNGTLAISNVQ--------RKEDASTYTCVARNDEGYSARSDLTVTVMEPPAIVPFSFGKDVINEGDFAQVMCSISRGDEPLLRPRWTVEPTDKQFAQGSDAKIDCKADGFP--KPEITWKKAVVNNLQMRPTPNDYQDFDASNDRNIKIIDGS--LIIKNIKKGNEGYYLCKASNGIGGISAVAK---IAVQAPPSFEIKKRTQTALISENT-VLQCEAKGEKPIGILWNMNNKRL--DTNVDPRYTIREESQE------DGML--STISIKRTQREDSALFTCVATNAFGSDDTSINLIIQEKPETPYGLKVVDKTGRTVKLSWQAPYNGNSNLTRYVIEFKPIKGTWAN---DVDHVLVPGDQTMAGVFSLRPATSYHFRVVAQNDIGVSG-PSDTVTIET 1046          

HSP 2 Score: 70.4774 bits (171), Expect = 2.619e-12
Identity = 88/375 (23.47%), Postives = 145/375 (38.67%), Query Frame = 0
Query:  355 DVEARDHGVTFICEATNEELGESVNDGITMTVKCKSFLNPLIDFIDKPIFDIDPTDTIFSVEEGSDLVVNISVRANPFDVTYRWAN--PYNENIPNITLDSDNIPRIYSIQNLLNITEARKEDAGRYKFKVNNSEGKSRLKIKIDVLYGATISDITDAVMVVTGETAHFECVVDAKPITADTISWSRVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRPRIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRPDTPTLLNVFNYTYNSVYLT 727
            +V   D   T+ C   +   GE+    ++ T        PL     KP F      + F   +G ++ V    +  P  V +RW      N N   + LD     R+  +   L I EA+ +D+G+Y   VNNS G   ++  + V    ++       +V  G +A F+C  D  P+   ++ W + G  +        G     L + +V KED G + C   N     + T    L  R  P  E   A     +       L C A+G P     W   GS       E ES+ +     ++     D     S L+I    ++  G+Y+C A ++ G+  ++  LNV   P    +L +      S+++T
Sbjct:  301 NVSPDDGFKTYKCRTKHRLTGET---KLSATAGKLVITEPLAS--GKPKFSSIVKLSAFQKTQGGNIAVTCPAQGYPIPV-FRWYKFMESNNNKKLVALDD----RVKQVGGTLIIKEAKVDDSGKYLCMVNNSVGGESVETVLTVTAPLSVEVEPKQQVVDYGRSATFKCHYDGNPV--KSVYWLKNGEPV--------GHTETTLRINSVKKEDRGMYQCFVRNDQESAQATGELKLGGRFDPP-EFVSAFDAVTTQPGPMVSLKCVAKGDPAPEVKWFIYGS-------EAESDGV----LQVGSFNSDNGDVVSYLNISQARTKYGGTYECQASSKVGLVSHSAKLNVNGAPYVRKMLPMKVVAGKSMFVT 643          
BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold691_size110934-snap-gene-0.31 (protein:Tk06103 transcript:maker-scaffold691_size110934-snap-gene-0.31-mRNA-1 annotation:"thyroid peroxidase")

HSP 1 Score: 72.4034 bits (176), Expect = 6.848e-13
Identity = 63/252 (25.00%), Postives = 111/252 (44.05%), Query Frame = 0
Query:  466 LNITEARKEDAGRYKFKVNNSEGK--SRLKIKIDVLYGATISDITDAVMVVTGETA---HFECVVDAKPITADTISWS-RVGFDMEGRTSMQNGTNSMHLTVYNVSKEDAGEFLCTANNGIADMKDTKSTFLLVRQRP---RIEESPANSKAASDKRKTAVLTCRAQGTPDVVFSWSRMGSVISQEPEEDESEEIEDEKYEIKYNVIDRMTWESELHIKNVSSRDYGSYDCIAQNEEGISRYAVYLNVTSRP 708
            L+IT  + +D+  Y+    N  G+  S  ++K+++    +I  IT        + +    F C+ +  P   D   W+ +   + EGR S+ NG    HL + ++   D G+++C A N +  ++ T +  L V  RP    I   P +  A   K  T  L C+A+G+P    SW + G  +           I D ++        R++ +  L + NV+  D G Y+C+A+NE        Y ++   P
Sbjct:  154 LHITNVQLDDSAHYRCSATNYLGRASSAARVKVNLALAESIPQITSKPRDAESQESSIVEFTCIAEGHPY-PDYSWWNNKRLINNEGRISVSNG--GQHLRIQDLRTYDQGDYVCRAENRLGKVEATAT--LTVNARPIPIAIINQPHDMSAP--KGTTVQLPCKAEGSPKPKISWMKDGRSM-----------INDRRH--------RLSSDGSLVVYNVTESDVGMYECVAENERERRTARAYFSIRDIP 379          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000004534 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 25
Match NameE-valueIdentityDescription
-2.261e-3136.43symbol:sns "sticks and stones" species:7227 "Droso... [more]
-1.737e-14333.69symbol:hbs "hibris" species:7227 "Drosophila melan... [more]
-2.083e-10128.35symbol:nphs1 "nephrosis 1, congenital, Finnish typ... [more]
-2.782e-9428.97symbol:NPHS1 "Uncharacterized protein" species:961... [more]
-2.628e-8928.43symbol:NPHS1 "Nephrin" species:9606 "Homo sapiens"... [more]
-4.522e-8928.85symbol:NPHS1 "Uncharacterized protein" species:991... [more]
-2.343e-8728.88symbol:Nphs1 "nephrosis 1, nephrin" species:10090 ... [more]
-3.763e-8427.77symbol:Nphs1 "Nephrin" species:10116 "Rattus norve... [more]
-7.368e-8427.77symbol:Nphs1 "Nephrin" species:10116 "Rattus norve... [more]
-7.368e-8427.77symbol:Nphs1 "nephrosis 1, congenital, Finnish typ... [more]

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BLAST of EMLSAG00000004534 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592847736|gb|GAXK01109808.1|1.149e-2340.96TSA: Calanus finmarchicus comp225879_c0_seq8 trans... [more]
gi|592847737|gb|GAXK01109807.1|1.150e-2340.96TSA: Calanus finmarchicus comp225879_c0_seq7 trans... [more]
gi|592847738|gb|GAXK01109806.1|1.252e-2341.13TSA: Calanus finmarchicus comp225879_c0_seq6 trans... [more]
gi|592847739|gb|GAXK01109805.1|1.253e-2341.13TSA: Calanus finmarchicus comp225879_c0_seq5 trans... [more]
gi|592847740|gb|GAXK01109804.1|1.513e-2341.07TSA: Calanus finmarchicus comp225879_c0_seq4 trans... [more]
gi|592847741|gb|GAXK01109803.1|1.514e-2341.07TSA: Calanus finmarchicus comp225879_c0_seq3 trans... [more]
gi|592847742|gb|GAXK01109802.1|2.004e-2341.24TSA: Calanus finmarchicus comp225879_c0_seq2 trans... [more]
gi|592847743|gb|GAXK01109801.1|2.005e-2341.24TSA: Calanus finmarchicus comp225879_c0_seq1 trans... [more]
gi|592929119|gb|GAXK01029426.1|1.124e-17936.20TSA: Calanus finmarchicus comp151307_c0_seq2 trans... [more]
gi|592929120|gb|GAXK01029425.1|2.068e-17936.20TSA: Calanus finmarchicus comp151307_c0_seq1 trans... [more]

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BLAST of EMLSAG00000004534 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 25
Match NameE-valueIdentityDescription
EMLSAP000000045340.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s237:37145... [more]
EMLSAP000000007954.636e-17938.56pep:novel supercontig:LSalAtl2s:LSalAtl2s1133:2264... [more]
EMLSAP000000047042.401e-7328.40pep:novel supercontig:LSalAtl2s:LSalAtl2s247:38389... [more]
EMLSAP000000115521.981e-2128.23pep:novel supercontig:LSalAtl2s:LSalAtl2s7:1384693... [more]
EMLSAP000000066453.446e-2025.86pep:novel supercontig:LSalAtl2s:LSalAtl2s367:2485:... [more]
EMLSAP000000050663.120e-1923.08pep:novel supercontig:LSalAtl2s:LSalAtl2s266:20529... [more]
EMLSAP000000002561.575e-1824.73pep:novel supercontig:LSalAtl2s:LSalAtl2s1038:1353... [more]
EMLSAP000000119144.815e-1822.00pep:novel supercontig:LSalAtl2s:LSalAtl2s840:4759:... [more]
EMLSAP000000054547.404e-1624.47pep:novel supercontig:LSalAtl2s:LSalAtl2s293:18528... [more]
EMLSAP000000020343.712e-1524.25pep:novel supercontig:LSalAtl2s:LSalAtl2s136:13217... [more]

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BLAST of EMLSAG00000004534 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|20177993|sp|O60500.1|NPHN_HUMAN6.737e-9028.43RecName: Full=Nephrin; AltName: Full=Renal glomeru... [more]
gi|325511343|sp|Q9QZS7.2|NPHN_MOUSE7.814e-8828.88RecName: Full=Nephrin; AltName: Full=Renal glomeru... [more]
gi|325511344|sp|Q9R044.2|NPHN_RAT3.572e-8427.77RecName: Full=Nephrin; AltName: Full=Renal glomeru... [more]
gi|75025812|sp|Q9U3P2.1|SYG2_CAEEL2.229e-7626.09RecName: Full=Synaptogenesis protein syg-2; AltNam... [more]
gi|49036496|sp|Q9HCK4.2|ROBO2_HUMAN1.107e-2725.37RecName: Full=Roundabout homolog 2; Flags: Precurs... [more]
gi|1028066215|sp|D3ZB51.1|TUTLB_RAT4.129e-2624.13RecName: Full=Protein turtle homolog B; AltName: F... [more]
gi|49036483|sp|Q7TPD3.2|ROBO2_MOUSE5.663e-2624.89RecName: Full=Roundabout homolog 2; Flags: Precurs... [more]
gi|1028066211|sp|E9PZ19.1|TUTLB_MOUSE1.026e-2524.49RecName: Full=Protein turtle homolog B; AltName: F... [more]
gi|158706512|sp|Q9UPX0.2|TUTLB_HUMAN5.586e-2523.43RecName: Full=Protein turtle homolog B; AltName: F... [more]
gi|49036500|sp|Q9Y6N7.1|ROBO1_HUMAN4.441e-2422.65RecName: Full=Roundabout homolog 1; AltName: Full=... [more]

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BLAST of EMLSAG00000004534 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 25
Match NameE-valueIdentityDescription
gb|EEC18933.1|2.100e-3842.68nephrin, putative, partial [Ixodes scapularis][more]
gb|KYB28870.1|1.835e-2739.23Nephrin-like Protein [Tribolium castaneum][more]
AHN56011.17.464e-3238.16sticks and stones, isoform C [Drosophila melanogas... [more]
AAF59040.21.070e-3138.61sticks and stones, isoform A [Drosophila melanogas... [more]
ABI31083.18.594e-3236.43sticks and stones, isoform B [Drosophila melanogas... [more]
gb|KFM57797.1|2.242e-2342.57Nephrin, partial [Stegodyphus mimosarum][more]
EEB10837.12.649e-2738.14Nephrin precursor, putative [Pediculus humanus cor... [more]
EFX79012.12.498e-17738.88hypothetical protein DAPPUDRAFT_305049 [Daphnia pu... [more]
EAA07064.55.534e-17435.74AGAP010742-PA, partial [Anopheles gambiae str. PES... [more]
XP_006564422.16.417e-15635.61PREDICTED: nephrin isoform X5 [Apis mellifera][more]

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BLAST of EMLSAG00000004534 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 25
Match NameE-valueIdentityDescription
gi|241839651|ref|XP_002415268.1|1.027e-3542.68nephrin, putative [Ixodes scapularis] >gi|21550948... [more]
gi|942305741|gb|JAN34803.1|4.246e-3341.68Down syndrome cell adhesion molecule [Daphnia magn... [more]
gi|942394527|gb|JAN79193.1|2.042e-2640.30Down syndrome cell adhesion molecule [Daphnia magn... [more]
gi|942305739|gb|JAN34802.1|4.097e-3340.99Down syndrome cell adhesion molecule [Daphnia magn... [more]
gi|942323125|gb|JAN43495.1|7.143e-3340.99Down syndrome cell adhesion molecule [Daphnia magn... [more]
gi|926606889|ref|XP_013782532.1|2.508e-3639.13PREDICTED: nephrin-like [Limulus polyphemus][more]
gi|1008431230|ref|XP_015833149.1|8.607e-2539.23PREDICTED: nephrin isoform X1 [Tribolium castaneum... [more]
gi|642915658|ref|XP_008190700.1|8.977e-2539.23PREDICTED: nephrin isoform X3 [Tribolium castaneum... [more]
gi|1008431235|ref|XP_015833152.1|1.381e-2439.23PREDICTED: nephrin isoform X4 [Tribolium castaneum... [more]
gi|1008431232|ref|XP_015833150.1|7.137e-2539.28PREDICTED: nephrin isoform X2 [Tribolium castaneum... [more]

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BLAST of EMLSAG00000004534 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 25
Match NameE-valueIdentityDescription
maker-scaffold14_size734282-snap-gene-4.112.532e-3750.37protein:Tk11303 transcript:maker-scaffold14_size73... [more]
maker-scaffold542_size141805-snap-gene-0.103.181e-2640.15protein:Tk09917 transcript:maker-scaffold542_size1... [more]
snap_masked-scaffold61_size441589-processed-gene-2.12.767e-2725.00protein:Tk06307 transcript:snap_masked-scaffold61_... [more]
maker-scaffold44_size478958-snap-gene-1.113.362e-2424.57protein:Tk06921 transcript:maker-scaffold44_size47... [more]
maker-scaffold68_size422247-snap-gene-2.113.449e-2127.13protein:Tk11392 transcript:maker-scaffold68_size42... [more]
maker-scaffold93_size381549-snap-gene-2.255.619e-1724.63protein:Tk04402 transcript:maker-scaffold93_size38... [more]
snap_masked-scaffold204_size260821-processed-gene-1.101.437e-1523.65protein:Tk07812 transcript:snap_masked-scaffold204... [more]
maker-scaffold108_size357748-snap-gene-2.204.351e-1523.56protein:Tk09444 transcript:maker-scaffold108_size3... [more]
maker-scaffold61_size441589-snap-gene-0.144.430e-1421.59protein:Tk06306 transcript:maker-scaffold61_size44... [more]
maker-scaffold691_size110934-snap-gene-0.316.848e-1325.00protein:Tk06103 transcript:maker-scaffold691_size1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s237supercontigLSalAtl2s237:371452..419819 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s237-augustus-gene-4.26
Biotypeprotein_coding
EvidenceIEA
NoteNephrin
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000004534 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000004534EMLSAT00000004534-700381Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s237:371452..419819+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000004534-687300 ID=EMLSAG00000004534-687300|Name=EMLSAG00000004534|organism=Lepeophtheirus salmonis|type=gene|length=48368bp|location=Sequence derived from alignment at LSalAtl2s237:371452..419819+ (Lepeophtheirus salmonis)
ATGGGATGTACATCTATATTTGCTCTAACCCCCACTCACAGTAGAATCGA GCCTGTTGAGGATTTGTAATTGAGTTGAAATTGAGTATCATTAAAATTCA AAAGGACTTAGGTTCTTAAAAAAAAAGCTCCAGAATCGACTCAAACTTTA CTTAAAAGTCTCAAAACTTTAATTAAGCTCTATGAAACGACTCGTAAACA GCTTTGGTTTGATTTTTGATGTGATATTGTATATGTATTAGAAATAGCAG ATACTGAAGCTTCAATATGACCATATCAAATCGCCATCCTTTGATCAAAT CGTAATTCATATTTTGTAGTGTTTATTAAAATAAAAAATAGGTATAAAAA TTTCTCAGATGTCATCAAGNAAAATTAATTATATTACCATTTTATTTTAA AGTTACGGTCACACATAGTTACTGAAGTAAAAAATATTACAAATAAAGCT CTTGAGTAGATATACTTTATTACATAAGTATTTGGCAGAGTTCCMAACGA ATCCCTTTTGTTGTAAGAATGAACTCAGGTGAAGAAGGAAACCATTATTT ACATACATTTCTGAGTAGACTTCTGAACTATCTCCTTTTTTATTGTTATG TCGTTTTGAATGACTATTCTACAAAATGTGTTTTCATATGACATTTGAAA CTGAACGATATCATATTTATTAATTTAATGTTCTCAATTATTACTAATTA TTAATCAACCAAATTATTTATGTCAACGTATAATTTTCTTATTCCCATTT TTATAATAAATATATACTCATTTCTATAAGTTATATATTACGACTAGAAT TGTCTGTATAATTTTTATTTTTTATGTAACTTTTAGGTACTAGAGTTTTT CGGTATACCGGTGCTAGTGCGGTACCGGGATATTGACAACGGTACTATAC TAACTGATATTGAAACCAGTACTTACAGTTACTCCAAATCATCAAAAACA TAATCCCTCAGATTTCTTAGCCCCCACCCCCAACCTTAACAAATTGTTTT ATATAGTTTTCTGAGCTATACCCTGCTCCAAACAATAATTCAGCGGATGC CTATCAGTGCATGCCAATTTTCCTAAACTCCATGTTTATTTATAATTTTA GTCGAAAAATACTACAATTCTCATATCCAAGCCCAGTGCTAGGATTTTTA TTTGGAGGGATTTTGAATATTTTTTTCAGATGACATCTCATTTTTCCAAT TCTAATATGGGCCTTTCTGATAAATAATTTGGTTTGTTTTTGATCGAATG AAGTTTATTTTTTGGAAGTAACGTTTTTGTTTTTTTAGTAACCTTTTGTT AACATTCTGGATCTCTTTTCATAGACTTAGTCGGAGAAAAAAATCACTCA GACTATTTGCTTATTTTGATTGCAAATTAAAAAATACTCAAATGAAGTCC CTTGAAAAATAAAATCCAATATGTCAAAAACTTGAGTGGTGTAGAACTTG GTTCGAAAATATTGGTACTGTGACAACATTAATAAGTAGCTACATTTTGT TACAATTAGCAGTAAAAATGAAAAGGAAAATTTGGTTACCCTGAGTATGC CTAAGGCAAAGATAAATGTGTGGGGCACTTTTTTTTTTCTTTTTTTTTAT TAACTTCTAATCCTTTATTTATGACAGCTATTTCAGAAGAGACATTTTTC ATTATGTTATATTATATTTTTTAAGATAAATCTACCCTAWGTTGGTCCCT TATTTTTTTAAACTAAACATGAAGCTGAGACAAATCAACCCAATATCCTC CCCTAAAACTGGCTCTAGGAAGAGGGTCGGTGCAAAAAAACGAAGCAGAC ATATGTGACCCCTTAATATAAACGAAAGCTATAGTTATACCTTTCAAAGT TCTTTGTGGATTATATTTGTATTATTTGACAAAATATTACAATTTATGTC AACAAACTTTATTACCTCTTGGGATGCCCCGGGAAAAAAACAATGCTCCT TAATGTTAATTTAATTGTCAAGTTAGAACGCACTACTAAAGCTAGTAATT AAATATTTCAATAAATGAACTTTTAACCTCCCAAGATACTCGAATCTTTT AGGAAATAAACAATTATTACGTCCTACTATGTCTTGATATTGTTAGATCT ACTTGCTTTCTCTTATTTTTGTGAAAAAGAGGACAGTTTTTGCCCACATA AGAATTCCTTTAAAATATACATTATACTATCTACTCAATATATCTGTACA ATTCATTAGCAAGCTAAACTTCTGATCGATAATTATAATACTAGATATGT AAAAAAAATCTCTGAATAACAAGAAAGGAATTTTTAAAGCAATGCCCTTT AGTTTATTTTGTCATTCAATGGTTTTGAAGGTAGACGAATGTGTTTAGTA TAAATGCAATCTTTAAGATTACATGCTGAAACATGTAATCTGAACGCACA TGTTGGAATAAGAATAAATTTTAAAGAAAACTTTCTAAATTAGCTGTATA CGTTACTTTTAATGCAATACCAATTAAATCATATGTTGAATTTTAATATC ATGAAATATACATATATATATTTATATGTAATTTCTTTTTAAACAGGAGA TAAATATCAGTTGTTATGAATCACTTATTTGCCTAACTACCAAATGATTT CCGGCCTTCTTTTTCTTTTTCGAGTGAAAGTTTTGTGGTAGAATAAAGTT ATTGAAAAACAAAAGAAAAAGGGAGTAAAATTCTAACTTATTCCAGCAAA GTGTGTCTGTTTCTTGACGTCTAATAGCTCCGAAAAATGCTGAGATGACT CCTATAATAAAACCCTATGTAAGTGTAATAGTTCAAAATGAATTGACAGT AAAAATTAATTGTTATTAATTAAAATGACGGATGCCATAATTATATAAAA GCATAATGAGACGTAGAAATTGTAACTTGTTCAATCAGTTTTTACAACTT TCAAATAAATTGCAACTTGTACAGTACGTATAGAAACGAATATTCTAAAT GTAGTGACATGATGCTGTATGGGAATTACCCATTGTGTTAAATGGAAAAC AGTTTCTAATTATGGTTTGAAGGAGACTGAATTATACATACTTACATATA AAGAAGTMCTCATTTAACCCTCCCCCCCCCCCTTTTATCTCTTTCCTTAA AAAACAACCTTGCTGCTTAAATAAAATAACTATTAGGTAAATCTGCAGGT TGGTAAATGGTGTATTTAAGATTTTGTTTAAAAGGGAACATGTTTCTATT ATAAATACATGGTAAAGTGTCTTTAATAAAAAAGAGTATAGACCCCTAGT ATCATCAATTGGAGTGCCATAGGACTCTGTTTACCTTGGTTTCTTTATTT TATATATTTTAACTGCATTGATCATTTCTTTGAAAATCATTTCTTGCAAC TTATTGAAATATTAAACACTATAACTTCTACTAATTTAATCCGTTGGAAG AATGTTCTGATTTTTTAGAAATCAGAATGCATTTTTAAATACAAGTTCTT CTTTTTTTTTATTTGATATTATTTTCGGAAATCTGTCTCACTTTTTTTAA CAAAATTTTTAAGTTGACTGACATTTTTTGGCTGATATTTATTTTAAAGA AATGAATGATTTTCTACAAAATAACACCTCTGGAGCTAGATTTGTAAATT TTCCAAAAACACTTTTGCACTTTTTCTATGTGGCAAGACATTTTTAGTAT CGGAACATCATAATCATAGAGGAAAATCATACTATCTATAGGTTTGTCAA AAAACAAAAACAATTAATGTGATCACCCTGACAATTTGCAGAATGTTTGA GAGGACAGAAGCATAACTGTTATGACGTTTCATTAATTTAGCTTCAAGCA GTTTTGAGAAGAAGGAAATAAACACAAGCCCAATTCGGTATCTAGCAATG ATATAAACAGGTATTAAAGCCAATAATGACTAACACATATAACTCCTCAG GGTTACTCTCCATCATATATGAGCAACCCCATTCGGGGTTACACTTTATA GTTTATAAGTATAACATCTTCTCTCCTCAAGAATGAAAAACGCGCTTAAA TTTTTTTTTAAACCAATACTCAGGTAGCAATCCTGTAAAGAGCCTGGCCC TCTTAAACTATAGTGTTATTTTTATCTATGGTCATTGGTCATAAGTAAGA AATTGCCGTTTATTTTCTCTTAAAAAATCAATTTATTATATATTTGCAGT TGTATCCATAAAGTATTTGAGCAACAAAATTGCCAAACTGCTCAAATCAA GGATCCTTAAACTCATGTATGAAATATCATAAGTATATTTGTTTTGCAAA AACAACATATAGCTGTTGATCTTTATTATGTTCCAAATTAGCATTAGCTC TCGTAGTAAACTTTATTTTTTTTTCTTTCTAGTTTCAATTAATTTACACA TTTTTAATTCATTCTCTATTTTCAGAATCAGAAACTATTTCAGAAGAAGA GGGACAAGATAGTGAGGGGGAAAAGACGAGCGGTCGAAAAACTGTTACAT CTGCAATACGATTTCGACCCACATCTGCAGATAATGGAGCTATATTTGCC TGTGAAGCGGAGCACCCAGCCCTTATTAGTAAATCCCCAATGCGAGTCAC CGTGGTTCTATCTGTCATGTGTAAGTRTTTACTGTTATTTATTTTAGAGT ACATATATGTTAATACACTACACAGGTTTATATTAGTATTGGCATGCTAC CAATATTTTCGGTCTGGTTCTAGTAACCAAATTGGTATCGGTATTTCAAT GTTTTTACGGCAAATTTAAATAAAAATCCAAAAGTTCGTTCAGATCAAAT TGAATACATTTACACGAGCATTTGTAAGATTTTTTTGTCAAATGTATGGG GATGGTCAGTTATGGAACTTCATATACCTGGAGTAAAATTAGCATTTTCC CTTTTTTTCGAATAACTAATTTTTTTTATTATATGCAATAATATATCAGA GTAACATAGTGGACTAATATATTCTGTATAACATTTTTAAAATGCAAAAC TACAAATTTCTACAAAAAATTTGTCTTCTTCACATTTTTTTTAATTACAA ATTATAATCTTATCAATAATTTATTACAAAAGTTTTAAATTTTGCATAAA AAACTCATTAGTTACCACGGATATCGTTAAATATGTGTAGTTCCTAAAAA GTACAAACCAAAAATGTATCCAAATACAAATAAAGTACTAAGAACTGTAA GTGTATAAAGTATATATAATAATATACATCTTGTGTTTTTTTAGCTGGTT CGTTTTTGTTTTTTTTGTTATTTTTTTTGTTATCTGAAGAATGTATTTTC TTGACCTTAGTCGACATAATTATTTAATTCCTGATTAGTGAACTTCCTTT CCTAAATAGATTCAATTTGATTCTAAACCTGATTGAGAATTCTTGTTTGC TCATAAAAAAAGGAGGAGAGTCACTTTAAGGATTTGTTGCGAGTTATAAT TGTAAGTCCTTGTTGGACTCAGAGTAGACTTAAAGATTGACATCGGAGTA ATTGTRCCTATCTCTTTGTCTATTTCCTTCTCCACCTCCTCCTTGATCGT AGCTGATCCCCCCTAAACCTTCTTCAAATTGTCATAGTTACCATGTTGAT TTTCCGGGCGTTTTTTTCTTTTTTAATTCCCATTATACACACAATTTTCA TCACTATATATTACAAGAAGAAGGATTCTATGACTCTAGTAGAAAAAAAA ATGGTGTCGATAGACTTTCATGTGTCTAAAATTCCATGGCCATGCCTCAG TCGTTTGATTACTTTTTTATAAAGGAAAACTTTGTTAAGCAAGAGAGAGT GTAGACATTCACGGCAAATATTTTAACACAAAGTAGTGTCACAAATACTG CATATAAATACCAGTTTAGTATAATGCTGTTTTAAGTTCGTCAGTATCCG GTACCACTATAGGTAGAACAGTATCTATAAAAAATAGAATAATATCCTTC TACTCATATCGAAAGCTCTCTCGTTTCATACTTAATAAGTATTACTTATA TATATAAAAATGATGCCAGGATAAAAATGACTTTAAATTTTTCCCTTCCT ACCTAAAATAAATTAATCCTCACTAATTTCACACTTAATGATTTTAAATA TCTCAAATATGATTAAGAAAGCAGGCATATGAATATACATATATATACTC CTATACATATTTTTTTCTCCAAAAAGCAGTTTTTGATATGTGCTATTCTT CAGTTGAGCTATTTGTTAAATGATCTAGAAACAAATCAGGATTGACCTCT ATTTCGAATAAGTCCTTCTCCTTTTAAAGACGGGGAAGAGGGACAAACAC AATTTTATTGTATATTATTAATGGGCTATGGCGTATTATTATATAATTGA ATAATAGTTCAATAAGCAATTCTTGGTTCTTCAGGATGGATAACTCTTGG AAATTCTAAAATTTATCCATGCTACATTTATGTATTTGTGTATGCACCTC TTGCCACATAACTTGTAGAAAATAAGTATATATGTATGTACAATATACAG ACCGTAGATTATAAATTTATGTGCATTTGCGTACAATGATATCATCATTA TCCAATTGTATTTAGATCAATAAATCCCCATATTTTTGAAGAAGAAAATA TGTGTCCTTTGTGATGACGATGTCATGTAATAGTGGTCGTTATTATATCC CCTCCTTTCCCATGTTTTTATTAGAGGCAGTTAAAACGATAGCAATAAAA ATAATGATTACTATTATCTTATTATAATGCGTTCCATATTTTATGGAATA CATTTAAATAAAGTCGTTGTGGAAATTGGGAGTGTACAAACCAAAAAACA AAACAAATGGAAAGTTTTCGTGCGTTTAACCTATTGTTCCGAGGAACAAT AGCCTATGAGTTTTTTTAAATTAAAAATCTTGAGTATCAAAAGTCCAGGA CTTTATAAAGGGAGACTTGCAACAACTTTTTTATTGGTGAAATCTTGGAT GCATTTAGGCATGAATTTGAATTCCGTTACTCAGCAGAAGGAAGGAGGTG AGAGAAGAGAAAGTTTGGAACTGTATTTAAAGTCATTTAATGAATGGTCA TTTGGATTGATCTATCCTACGGAGCAATCGGATTTATGGACCTTCATCTG CCCTTCGTTTCTAGAAGTTTATATTTTTCCATTTGGATATTYTAGCCACA CCGTATCTCACCATTAACTTATGTTATGTTCAAGGGTTCTCACCTCTTTG TACCTCACTCACTGGATCTCATCCTCTTTAATAGAAAGAATTGTTCTCTG TGCAGACGCAATTTAGTACCCATGTATGTGGTTGGGATGTTTGACTACGT GAATCATTGATTCTATGCTCAAGGATAAATAAATTGATACAAAACTTGTA CGAAATCATCATATACTTCAATAAGAACTACAGAGTTCTATATAAGGCAG AGTACTTGACTGAAATACCCATTGAGCAAGAGAGTTTTTTATTACAAAAA TAGACCTAATATTAAGAAAATCATTTTAACACATCATTTTCCCTTCATGA TTTATAAGCTGCTCGGCAAATTGTGTATCGTCACCAAATCATCATCATTT TAATATAGAAATAAAATCTTGAAAATTAGCTTATGTGAGCACTTCACAAT TTCTATGACCCTCGCCACTTCAACTTCAGCATCTTGTAGATCTGAGATGT ACTACCTTTTGGCTTTTGCTTATTCATGATGACAAGATGACGAAATTTTT ATTATAGTTAGTTTATGCACAAGGAATGGCTGAACCAAAATGCCAAAAAA TATTCACCAAATTCCAAACCCAGTATATTTTTGTGTTAGTGAGGTAACAC CGTGCCCTACAAAGAAAGGAACAAATTGCACACGCCATGAGGAGAAATAA TAAAGTAACGTCATTACTGTTATCACAAAACGTGATATCTTATGTAGGGA TGAGAGATCAGATCTACCATGAATCTATTAAAACTAACAGTCACGTTTCG CCTCTGCGTTTATAATCCTTATTTTTTTAGTGATAGAAGATCGCCATAAT TGACAATTTTTTAATATCTTTATTAAAATATATATTACGTGAATAGCATT AAGTAATTTAATATACATAAAAATACATATATAAGATAATAATATTATAA AGCAAAAACTTCTGCTCTCGACTTTTCTATACCCAACTGCTGCTAAATAT TTATTTGATTTACGATCTTTCTATGTGTGTGTGAACTGGAAAGAAAATGT AAGGAAGTCTAGAAAGGTGGAGAACAAAAACACATAATATACACACATAT ATATATATATATATGTATACACATGGAAAGAAAGTAAAAGGAAAAACTAT TTTTTCCTTCTTTGCATCATTAATAAATTTAATTGATGAATAAAAATAAA AATTAAAGTATCATCACTTAGAAAATATATACATATAGGCGCCTACAGAG AGTAGATAAGAGAAGGAGGAGGAGGCTAAATAAATAGTTAATTATTCATT TGTCAATTTTAAAAGTTTGTGAAAATAAATTATTATGTGCTCTATTTTGA GTCTTGAAAAGATTAAAAACTCTTCCCCTTTCTAGTTCAAGACACCATAC CTATGTACATATTGATATGAATGATAACTAGAGTTTTAAATTTTAGTTAT TTTTTGGCTTGTAAAAAAGAAAAATAGGGAGCTCGACAATTTGAATAATG CTTGGGAGAGGAACAGCTTAACTGTTATAGCGTTTTATTAGATTCGCTCT GAGTAGCTATGAGAGGAGGGAAATAAACACAAAACCAACTCCGTATCTAA AAAGAAAAGTCCCGTGATATGTTGTTTTAATTCACCTCATTGTCAGTGTC TGAAAACACTGATGCCAACAACAAAGAAACTTACATACTATAAAATGACT CAGATTTACAAACCTACTTATATGTATGATACATGATATTTTTGATCCTC GATTTATGCAGTGTGACAATTTTTGAGAGAAAAACTTCATAGCAAGTGAT ATTTTATGGAGGGGGAAGAAACATGTTTACAATCATACGTTATAACAGGT TTTCACAATTGGAACTTCTTGGAGCCATTGTACAATCAAGTGTATCGTCA AGTACTCTACGAGAGATTACTACAAGCTACCATTGATATATACGAAACCT GACTCAATATTTGTTTTTTACTCATAAATGACAGAGAGTAGCCTTTAAGA TTGGTTGGTAATTCTTGTTGGACAGAGAGTAGAATTAAGGATCGACATGG GAGTATGTTCTTTGAATTGGTCAGATAGAGTTGCACTTATTCAGTATAAG TAAACATGATACTACTTTGATTACTCCATCTGAGCAAGGAATGGTCTTTG AAGTCCATATGCATCCTTCTCTAGGAATGACAGAGTATTGAGCTTACGCA GATTGAGTTCAAGAGAATAATGTCTCCGGAGCGCGATGTGCAATGACCTA CGTCGTAGGAGTAATTCTTGCCTATTTCCTTGTTTATTTCCTTCTCCCCC TCCCCTACCTCTTCACAGCTGCCTATAGCTGATCTAATGAAACTTCATGA CTGCTAAGCTGTTCTCCTCTCAAACATTCTTCGAATTGTCAGCCCTATCC CGTTGATTGTCGGTTTMTTTTTTCTTAACTTACCCAAAATACACCCGATT TGTATTACTAATTGTAATAATAGGTGTATTAAAAAATCAATAGTTCTTCT CGATGATTAAAAGGTTGAGAATCACAGCTTTAGAATATGGCGTATTTGAT ACATGTATATCTACATTTTTTTCTTATCTAAAAAGAGTTAAATTATGTCA ATTTTAGTATCTAATGTTCGAACCAGGATTTATGATTTTTATTCTTGTGT CATATTTATCTTGACATATAAATTGAAAAATATTGACAAAAATTTATTTA TATTTGATTTAAACTATATACCTACATAAATAACTTTTAACTTGATTGAT TTGAAGAGAAATCCTTCGTTTTTATTATAAAATACATTGTACATAACAAA GTATAAAAATGTCTTATTTTTCTTACCACGCAGATCCATCAGGACCTCCA GAAATAGAGGGTTACATCCAGGGGGAGACCATTCGTATGGGGCAAACAGT GTCTCTTATTTGTGTGTCTCACGGAGGAAATCCTCTTGCAGAAGTTGTCT GGTACCGCGGGGATGAGCAAATTGATTTCTCTTACTACACGACAGGTCGT GAGTCTAGGAATATGTACACTTTTGTTGCTTCCTCTGAGGATAACAACAA GCGTTATCGGTGCAAAGCTAAGAATACTGTGAGTGTGGAACCCTTAACAG CGGATATCGTTTTATCAGTTCAATGTGAGTATTTATGCATGTATATGGCC TTTATGCGGGAAAAGTATATATTATTCACGGTTCTAATTGGCTATAACAA TAAAAATTTATGAGATGAATTAGTAGATATTAATGAATGTCCTTAATTTA TGGTCTTCTCTCATTATTTTTGCAAAATGCATCATTGGACAAAGAAAAGA AAAATAATAGCGAACATTTGCATGTATATTATTTTCGATATTTCAAATAT ACATATATGTATATTTTTTCCCATTTCCCTTATATCTGTGTAAAAATATA GATGCAGCTCGAAGAATGAGAATATGAAAAATATTGAAAATCAATTTATA CATAATTAGCAGGTTCTAATGGTCTTATATTGAACATGGACATAAAGATC TGTTTTTTCCTACCTATATGTGCATATAAAAAATATTGGTGCATAAAATA GCACTCCCCACCTATTGTAGGTATAGGGGGTACATATAACTAAGGGAAAA TCTTGAAAGGCTTATAATTAATTAACTAGATGGGGTAGAATCAGAAGTTT TTTATTATTTGAAAGCTACATATAATTGCATTCAATTTTCATAATGAGAT CCGCCTCCCTTGATAACCTACTTAGCAAGGGGATGCAAGCATTGGCAGGC AGAGGACCYTGTGCGGGKCCMTCATTTCCATTGTACTGGTTCTGCACTTT AATTTTACTTTCCATGGAAGTCCAAAAAATTGCAGGTCACTTTGGCCACA AACTATCATTTCTTTTGAAGCCAAGATTTTTGGCCTACAAACAGCCCGAA TATGAACCCATGCAATTTCTAGCTCTGGAGGATGGTTGAGGATGAGGCTT GTGCCAAGTATCATTGCCTATGGAGGCCCTGAAAAAGTGCATTAAATCTA CATCGACTAATCTGGATCCCCAGAACGTCTCCCGATACCTTCAAAGCTTC CGGTTCTGTGTGGCCCAGGTCATCAAGAGAGTTGGTTTTCAGTATAAATA TTTAAATGTCATTAAATGTAGCTTTMAAGCAATAAAAAAAATATGGTTTG TTGTAGCCTATCTAGTTCGTTCGTTGTCAGCCTGTAAATATTTTCCCAAT TTATTTAGACCACCCTGTACATAATATTAAATGAATAAAATCCAAAAGTA GTGTTTGTGGACTCAAACAATTATTAACTTCATTTAAGTCTATAATTTTT CCACTCAGGGGAAATAGTAAGTCAAATTTCAAATTCGAAAAAAAACTTCA TATCTTATTGAATTGGATTTATTTGTACCACTAAATCTCATCATGTATAT TGGGTATCATGTTCCTAAGTAACTTTTTAATCAATTAAAATGTGAAATGT TATCCTTTTAATCTCTGAAAAGAAAGACAAGACAATATATTCTATAGTTT TTGTAATGAATTCCACAATTTTACAGTTACTAACAAATATTAGGTAATTA GGGCTTAGAAAGCAAAAAAGATCGGCCGAAAAATGAAATAGCAGTACGCA TTCATAATGATYTAATTTGTACTCTTTTTTTTTTTTTTTTGTTCTTTTCA AATGGATCTWTTTGCTTCGGTTCGWTTTGMCYCCAGCCGAAACAACAATA TCTAAAACTCGATACAATCAACTAACATGAATTATAATATATATGTATTT CTTCTTAGTCGCTCCGGATAAAGTCACCATTGAGGGACCCACTGAGGCAA ATGTAGGCGATACGCTCTCTTTTGACTGCATCACAACCAATTCAAATCCT CCAGCCATCGTGCAATGGGTCGTGGATTCTCGCTCTGCTCAAGAAAACTT CACTGTCCGAACAGAAAGTGAGCGAGGTGGTTGGGAGACGCGTTCAAATA TATCTGTTCTAATTGGTCCAAATGATAGAAATAAAATCATTAGCTGTTAT GCAATCAACAATGATCTTGGGGAAACCGTTGTTCAAACGCATACGATCAG TGTTTTATGTAAGTTTTCTACAATCTAAAATATTATGTATATATGTTTAA TGTTAAAAAATGGAAATGAGAATTTATTGTTGGATTATAAAAATGATTCT ATACTCAGAAAAATAAACTTTTATTATTAAATTTATCCTATTTTCTTTAT TCTTCCAGTCATAAAGTACCATTTTTTTACTTTCACTTTATCATATTCCA TCCTTTTACTTTTTTTATTTTTCTACTTAAGATCAGAATAATATTTTTTT AAATTTTACAAACCTAAATGTTAATTTAAATCTAAGTATGTATAAATAAA TTTTTTTAAACAATTTTAGCAATTTTAACAAAATTATAATAGATATCTAA TTATACGGATACGTACACAAAGGGAATCAGGATACATACAAAATATATGT TTTTTCAGATCCACCGGGAAAGCCCAGCATCACAGGCATAGACCCTGGAA CCATCATGGATGAAGGCCAAAGTCGAAGACTCACTTGTATTTCAATGGCA GGGAATCCTTTAGCTACTCTAACATGGTATCGTGGAGATGAAATCATTCA AGGAACAACTCAGGATGACGATGAGACAGAAACTAAAAACTACGCTCAGT CCGAACTTCATTTAACAGCAAATTACTCTGACAATGGAATGGTGTATCGC TGTGAAGCAAGTAATCCTGCAACAGCACAAGAATCTGGACCCCTCTCTTC CACTATAACATTGTCTGTACGATTTAAGCCCATTACAATTGGTGAGTTGT CGTGTTTCCCCATATAGTTTTTTAATACTGCTCTAATGGTTCTATTAGGA TATGAAAATCTATATCTTATATAGATTTTTACATTGAATTTTATGTATAA CATTATATAGAATTCCTACTTTTTATGTATTCTCAAAAAAGACTCTGAAT GAAGTTATTAACATAATCTGTTTTAAATGATTATGTTCAGATGTTACCCC ATAATTCTACTTAATAATGGAAACAAAGATTTTTTATCTTCCTCCTGAGT TTAAATCCTACATAACTATATATATCTATATTTTTGAACAATGTAATTTT TCTTTTATAGCTGTAAAATCGGATCCGGAAGTCCCAAAAGCTGGTAAAAA GGCTTACCTCTTCTGTGAAACAGGTAGCTCCAATCCGACTGCAAACGTTA TTTGGAGACGAAATGATGGACTTTCTATTGAGAATCAAGGAGAAATTCGT GTTAGATCTGGGCCATTTGGAGGAAATTTGACAACAAACGTTCTTGGGAT TGATGTAGAAGCTCGGGATCATGGGGTTACTTTCATTTGTGAAGCTACAA ATGAGGAACTCGGTGAATCTGTCAATGATGGAATTACTATGACTGTGAAA TGTAAGTCTTTTCTTAATCCTTTGATTGATTTTATTGGTAAGTACATATT TAATAGTAACGACAAAATAAAAGTTTTGTAGTCATAATTACAAAACTTTG TTAAAATTTTGAGATTTCTATTAATTTTTTTACAACAAAAGTTGAGAATA TTTTAATTTATATGTTTTTTGAAACTATACTAACTTCCCTTTAAGTAAGG CAAAGAGTTAACCTTATTTGGATCTGACATTAGATAGTACCTTGACTAGC TGCTCCGAGACAGATCCCTTCCAACAGGGGTGGGGCAAGTGATAATACCT GTCAAAACTAGAAGGATATCAGTAATAGAAAAATTTGATGCGAAATCTGT TTTATTGCATGGAATAGACAGCGGATATTGACAAAATAGAAAATTAAATA ACGTGAAAAAAATTAAATGTCTTTAATTTTTTTTAATGGTAATTTTCCTC CTCACTGTTCAGTTGGAGTTTCCCCAAATAGATATTAGTACACATTGTAG TAATGCAATTATACATCTGACATATGAAATATATGAGAATTGATATACCT TTCAAATTTATATTCCCTTCTATAAAGAGTAACTGGGATTGGAACTTACG CACTCTGAGTTCAAAAGAATTTTATCAAACGTGCCAAATGTTATAGATCG GAGTTACCTAGGTATTGCATAATATGCAGTTTATACATTTCTTCTATGAA TAAATAAAAGATTATAAGAAGAAACTCATACAAAAAAATATTTCAGCCCC TCAATTCTCCAAAGAAAAGCCCTAAGAACAGTCCTGCCTCGAGGTCCCTT TCCTCCATCACTAATACTACATACATACAAATATATGATATAGTTTATGT ATTTTGCCTTTAAACGAAAGATAAAGATTTAAGACTTCTATATTTCAAGA CTGATTTAGCGGGTAGGTTTTTTTCTCATTTCTTTTTCTTTCTCAACTTA GATTTATGGCTTAACTCTCCCAGCGACCGTACATACATACACACAATTTT CTTGTTCATTAATGTTGTATACAGTACAATGCTGTTCATCCTACATATAT TTCACTCAAACTATATTGGTTGACTCAAAAAATTAATCCTTAATTATGCA TGTACGAATTATAAATCTCTCTTATTTCTTGATAAATCGAATAAAAGAGA ATTATGCGTATAGTATGTATATAGTTTGTGGGTCCCTTTTTTTACTGTAT CGTAGTTAAGAAAGTAACTTTCACAATTACATATATAAGTGTATGTATGT GCTTCTTAGCTTGCTGTTAAGTAACTTTATTGTGATGAATGGCTCTCTTT CTCTAAAATGTTTTTTAATTGAGCTAAGAAAAAAATGTCCTCTTTAAAAA ATGACTAAATAATGTATTCATAGAGGATCCTTGTATACTCAATAATTTAT CACTATGTGAGGGTGGCCAACAAAGTGCGTACACAAATGTTTGTCATTTT TTTAACCTACAACCCCCACGTACACATTTGGTACACATACAGTGCGTACG CTGTAAACACCTGCATGAGTGGACTATTGCCCAAAACATTTCCCAAAAGG ACAATTTGTGCAACGGGCATTTCGCCGAATGACCATTTGAACATTTGGTC TAAAAAATGATATTTAATATATTGATATATGTTATTGCATATTTTAATTC AATTTAAAATGAAATCATGAAAAATATCATATATATCATGTGGTAAATTA ATTAGTCTTATTCTGCAAAATTTTTTTTTATTGATATCCAATAAAGATAA TTTTATAAACAACACACTGTAGCATGTAGTTCAAATCTTTATCTCAAATC GTTGTATAAATGTTTATGCATATTTAATGAGGAAAGAAACGATCTAAAAA AAATATTCGACACTTTATGTAAACACCTAATATAGTTAATTTTGGACGAT ACACTCTAATGTATATGTTGTGTTCAAATTGCGATGCCTTTATAAGTGGC AAATTGTCTCGGAAAATTTACAAATTCCGATTTCGTTGCACTAAAATGCA TTCTTTAATTACAAAGTTGATCATTCTAATTTCTAACTATTCATGGATGC TATCATTTAATTTTGATAAATTCAAATTACTTGATTATCATTAATTTATG AGGTAAATGCCCGTGGGAATTTGAAGTTTGTACTATCCTATATAATACTT ATATTTCAGTCATTTGTATCAAAATAGTGAAAAATTACTCCATTAATTTA TATAGACTATCCTTGGAATATTCAAATATTGTATTATCCTCATCTTAAGT ACTATAACACATTCATTTAAAATTGAGCATCTCATTTGATTCATTTTGTT ATTGTTCCTTAAATTGGTCAGATGGAGCTGCATTGATTGAGTATAAGCAA AAATTATAGCATTACAATTGCTTCATCTGACTTATTAATTGTCCTTGAAG TACATATATGTACAGTATGGATTTTTCCTTCTTTAGGAACGACCGAAACG CGAACCTACATGGATTGAGTTCAATAAAAAAATTTCCCCTGAGCACGTTG TCTATTGAGCTACACTGATAAAAAATTAAGATAGTATCTTTTTGTCAGAT GTCGATTTTTCTGCTTATGTTCTCATTATGAGTGTTCTAATCATTGAATG GTTGACCAAGATCAGATTTTTTTTCCCCGAAAGAAAACCAAGATAATCAA TACGAGCATAAATTTATTGATAATTTATTTTTAAATTTTATTTCTTTTAA AATATTAACCCCCCCCCCCCTCAAAAACCGACGCCGATCGGAGCTATATG TCCTCTTTTTGGGGAATAAAAATATGGCAATCCTATTATAAATAATTTAA AATTAAAAGCAATTATAGATTAATAATTCTATAGTATAGAAGAATAGGCT AACAAACCAACTAATTTTGGCCTTGCTGTTTTGTTTCTTTTAGAAGATTT TTTTCCGATTTAAATAAAAGTATATCAACAAATATCACATAGTTTAAAAA TAAGTATAGAGAAATAATGAAAGTGAAAGCAAAAGGTCATACAACATCAC ACTTGACTTTGAATATCCCCATTTTTCTAAATACTTAACTTTATAACGGC CTCTACGTTAAAGAATGATCAGAATTTTGTGATTTTTGAAGTTATTTATC CACTTCCCAGAACAAAATTATTTTCTATTTATCCAAATCGGTCAAAATCG AAGATTTATATGAAACGACGTCGCTTAATGGAACATGTACTAGGGAGAAT TTGGTCTCTTGAGCTCAATGTGCGTAGGTTTGCACCGTGGGCGTTGCAAG AGAAGGAAATTTACTTTATGTATATGGACTTCAAAGAAGATTCCTAAGTC AGATAGACTAAATGTAATACTATAATATTTACTAATACTTATCCATCTGA CTAATAAAAGGAACAATTAAAAAAATATACTTAAGATTTGGTGTTTTCAA TAAAAAAGTATAGAATTTTTAGTAAAAGTAACGGATATACGTTTAATACA AATAAATGGTTTTCTTTATTATAAACATCTAAAATTAGATTTGTAGTTTG TCAAACTTTAAGGCCGAAATTTGTTTCCTTTTTTTTTATCATTTGGATCC ATGACGTTTTTGAACCAAAAAAGTTGTCAAATTGCCCAAATCAAGCATTA TAATTCTAATGTATAAAATATGAGAACTATGGCGAAATAAGATTAATTTT ATCCATTAATATTTGAATATTCATACTCAAGGAACTAATAACTATCGATT AAGGTCAACTGATTTGGCATTTGCTTTTTTTGTCGGCATTATGGTCGGCA ATTTAAATGGGAAGAAAGAAAAGATAAGATTTCCCGAGAATTCACTACTT TTTTGGAAATTATCTGTACATCCTTCCACTCAGTGCAAGGATGATAGCTT ATAAGCATAATCGGTCAGTCTACGAGAACTCTTCCCGAAACGTTAAAAGT AGAAACTATTGAAAAACAGTAGTTGTTGGAAGTTCCATTCAAAAACATGA CTTTGGTTTTCACTTCCAAGATGGTGATAGTTTAGAGTAGTTTTATAAAA GTTAGCCTTTAATTTTGTTCCATTTTTCATTTTTTTAACCTTTCCTTCTT TTGTTTGCAACCATAAATAAATATGGTTATCTATTAATCATAAAACTGAC AACAAATTGAAACAGTGTGCTGGAACGAATTTCAACATGCCTATGTGCCG ATTTCCGGCATATAAGCATGTCAGATTAAGCCAGAAATAGCCGGTAAAAG TCGATAGTGGGGCAGATACTGGATTGAAATTAAAACCATGTGCTGGAATT CCTTGCCGGGAGTAACATGATATACATAAATATGCAGATTTAATGCGAAA ATAATAATTAATTTATCTCTCTGCTTCAACAAAAATACTCACTCTTGAAT ATGTACTTAAGAGATAAACTCGATTTAAATCTTTTTTATTCTCTTTCTCT CTACTTCTTAACGAACAAGAAGATTGATTTAATGCATTAAATTAATGCAG TTCCTTTATAGTTATTATAATTAACGAGTGAGCCTCTGTTCTTTGTGTGT TGAATTAAAGGGAATAAGGATGAAGATGGAATGAATTACATCCATAGACG CAAAAATGAGATTTTATTGACATTGATTTAATAGCATGAATATACCTTTT TGAAGAAGCATTTTTTCTTATATTCTTATTACAAAGGATTAATGATTGTG TCTTCTACTGCTAGAGAGAGGAAGGAGKATGTCATTTGAAGAGTTCAATG GGAAATATCCATCAATTGGGAATTTAATTTTGATTTTCATTACATAAATA ACGTTTACATTTTTTATATAACATTTCATATATGTTAGTGTTCTAAAAAG TTTTTGCTATTATTAGTCAGATACCGTGAAACCAATAAGGTCTAAGAAGA KATTATATCTGTAAAACCATCTGGCCTAGGAAATATCTTACAGTTAATAT ACATAAAAAATGTATATTTCCTTCTCAAGGAGCGACCAATATTCGAACTC ACGTAAACTGATTTCAAGAGAATAATAGGTTGTGAACAATTACTTACTAT TAAATAACTCCAAGAGTTAGGAAGAATGACTTAGTTTTCTAACGATTAGT TAACTGCAGACTTTTTTTTTACTTTCATTTTAGCAGAAAAAGTTGAGATG GTCAACTTCAATGTTGTACTTACACATTAGTTATTCTGTATTCCTCTTCA ACTTTTTTTTACTTGATGAGAATGTAATTTTACTTATTCATGTTTTGAAC GTTAATCTGCTAATCTTGAATATTTATTGGTGAGTAGTCCAACAGTTGGA GTCAGATAAAGAACTTAAACAAAGAAAGGGTAGGGAGAAATCCAATAGTT ACTGCCCTGTTTTGAAAATGTTCCGAATAATTTATTTTATGAAGAGCCAT TGTCTTGTAAAGGTAAAAAAGTGCATATTTTCTACTTTATATGAGTTTCA AAGTGTTTTGCAACCGTAGTGGCAGNTTTGTCCCTGCTAGTTATATGAGA AATGTTAAAAACTCAYTCCATAATTAACTAATAATTATTCAATTAGGCTA TTTGTTAGCAAAAATTTATAAAACCTCTTTTATTTATAGATTCATACATA ATAACAATTAGCAGATTGGCAAGCAAGATCACTTTCAATAATATGTATGT AAAATCTATTTGTACATTTAATTAATATGAATTTAATGTAAGTTATGAAT TATTTTAGTTTGCATTCCTAAAAAGTAAAGGCAAATGAAAACAAGGGACA TAGTTGAGTGAGATTTGTTTAATAATTAAAAAATAGAAGTACATTGTATA AGTTTGCCTGCAGGTTTTACTTTCTCTTTTTATCTCAAAATAGGAGACAG TGACAGGGGATATATCAAATTCAATTATTCCAACCTTTTAATAATAAGGA AATAACTTCAAAAAGGGGAAAATATGTTATTAGGAGGATAGGGCCATGAA AGTAAATGGAAGGTCTCATTATGATGAAAAGGTTGTAAATTAGCCTCTTT TTAAATATTCCGGTATTGCAGCTCGGAGTCGACCCGTGGACTGAGGCATC AGTCCGGAGGAGATTGCATATCATTAATTATAGTGCTACGACTTTTTCTT GTATATACTATGAACCATTACAGCATTATTACAGGATGAACATAAAAAGT ATCTTAGAGATGAACTTCTATGTCATCAAATCCATTAACTTCGGAAACAT GATATTACTATAAACATACATAAATAGAAAGATACACTACAAACTCAAAT GATGGTCTAATTCATACATGCAACGTACCCATTAACTGATTAATAAGGAG AAGCTCCACGTACATCCTCCAAAGCATTCTTCAAACTATCTGGATACCAT GTTATGCCATTTTTCGGTTTTGCTTTTTGTACGTACTGAAAGTGCTTAAC ATTTAAAACTCTAATTATGACTACCCCAACTCATTCAATTCTTGAAAAAC AATAGAAAGGAAAAATTGTTGTCATCAATACATCACATAATTATTGTACA ACCGACCAGTTATAATAGGTTTTCCAAAAAGAAGGTGGAGTAGCCTTTTC ATTGATGAATTGGTTCGCCCATGCTACGATCTCTATTCAACTTTTCTATA AATACCAATAAAAACCACACAATTCATTTGCTACAGAGCTACATTTWTAT CACGTGTTTTGTWTCACTACTTTTTWTTTCTCTCTTAAGTTNACTACTTT TTATTTCTCTCTAAGAAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA CCTTGAGTTGTTTTTTGTATGTTTGAGGTTAAGACAAAACTTTTAAAATA ACAAATTCGTCTCTTTTTTCATGTTGATATAATTTATGTATTTGTATGTG TGTATATATATTTATATATACCTAGTTTGTGACACTCATTTACAATTTCT CCGCAACAACAACAACAAAAAAGAATAAACGAAAGAAAAGTTACTTCGTT TTTTATTACTCTCACTTGACAAGTATGTATGTACTTGTGTTGTTGCTCGA AGGAAATATTCATTCCATCACCACCAACATGTTTAATTTTTTAATTTTTT TTTTCTGAAACTAAAATAAGAGAAAGTTATGTTAACTTTTATTCTGCTTT AACACACCAATATACAATGAGCATAAGTATAAATATAAAGAAGCTTTCAA ATGTAAGGTTACAAGTACATATACAGGGTTGATTTTTGTACAAAAGTTTA TTATTTTCAATTTTTCAAGAGTAAAATTTAAAAGAGGGGTTTTGTGAAGA CTTTTATTTAACAAAATCTGAAAATAGTGTTAGGCAAAACTTTTATTCAA TATGTGAGCCATCAGCTCTCATAGTTGTTTTTATACGAGACCTAAATCCC CCGCATATCTTGAATATTAAGTAAGGCTAGAACTTTGTCCACTCCTTCTT GATGGAAGGCTCTAGAGACTGGGCACTCCTCTGAAAAGTAGCCCACACCC TTTTCTCCAGACGTGCCTAGATAGAGTAGCTCAAGGGATTTGCGTCCGGA GAGGAGGGCGACTACATTTTGAGGTCCTACAACTCCGGAAAGTTATCTCC TTTGAAGGCCTGGGTGTGATGACACTGAGCTCCTTCTCGAATAAAACAAA GTTGTCCCCAAAAAAAATTATSTTGCCCAATGGTGCACCTTCTTATCCCG AAGTTTGATATATGAATGTGCATTGAGCCGCTTCTTCCTTTCTACAAAAA TGGGAGGGAAGACTTTGGGATACTAGATTTTTTTGTTCTAAGAACATGGT TCACTGAAACTGAGACATTGCCTAGATGTTGGTTGTGATGTAACGGCTGM TCTGTTTTAAGGAAAAAATCTTTGTGAGTAGAGACTATTTAACGCTTCGG GGGATTTATATATGTTTAATTGTAGAAGCCCCATTATTGGAAATAATTTA GTAATAATTTTCATTCCTTTATTAAGAAAAACGTTAAATCATGTTACCTT ATTATCTGTTTCCATGCGTTTCTTTTTATTTTTCCGTTACAATCATCATA TATTTAATATTTATGTATGTTATATATGCTAATACTTTTTGCTACAAGAT ATTTTATTTGAGAGGGGGGGAGGGGGGGCTTTGTACAAAAACCACAACTG CTATTCATGCATCAAAACAAATAATACGTGCCTATGTGCATATTTTTAAA TACATAATAATAAAAACTGCATCATCCAATCTGTAAAGTATATTTCGCGT TTAGCTTGTTATTCTTATTTACATACGTAGAGCCACAGAATATAAACAAT ACATACGTATTCACAAATATTGTCATCTATTGAGTTATGAGTATATGTAC ATATATTTGCACTTTGAGTGTGTACGTGAGGTTAAGTGCAATAAAGGTGA CTTACTTTTTTATATACATTATTGAAGTTTTTACTTTTGAAAATTCGCAT TACATATGTAACATGTTAACATAAAAGCAGGCCGCTATGTTTTTTGTGAA AATATTCTAGCAGGCTATAACAACCCATCAAGTTATGTTCCGTAGCCCTT TGTTAGCAAACATCCTTAATATAACGGTCATTCTGAGCTAGACTATAATA ATTAGGTCATTTAAATTCTGTATCATTTTTTAAATTATTCAATTTATATT GCAATCATAAAATATCAATTAAAATGCTAAAAATATTTGRACAAAAAAGT GACATCCATAAAAAATAACCACTTACTTTTTAAATAATATTAAAAACATG AGTTTGGCCTTTCAAAAAAGAATAATTTGTTATTCCTAAAGTAAAAAGAG AAAAATATTAGTTATTTTTAATAAAATAATTATCACTTTATACAAACTTT TGGAATCAAATAAGTACCCATCCCTTTTGAGGAGGTTGTGTGAATTATAT TAAATATTATCTACAATATTACATTTTTTTTTGTATCATTTTATGGCTCA CATTGAAGAAAAAAATACCCCACAAATAAATAAAAAAACGGTTTATAAGC AAAACTGATAATTAAATGAACAAAACATTGATTACAAAAATGTAAAATAT GCGAATAAATCTTAAACAAGGTGTTATCAAAATAAATAAAGAATTGCATA TTATCAAATTTAATATTTTAGGAGTATTCTGAAAAGTATTGGCGATAAAA TGTAGATTCAAAGGAATAAGAAGTATTTTATAAATCTTGGGGTGTATAAT ATCCCAGATCACCAACAAAAAGGTTAGATTTTATTAACAAATGGTTGAAA TACCTTTGGTAGAAGCTGCAGTTTTAAAGTGTTTTATTATATATTACCAA AATTCTATGTATAATATGAAGCATAACAATAGATTGAGATTTCATCAAAA TAATATAAGGCCTTCATGGAAAAGCTCAAAGCCTTATCTTATCATAAGAA TACATAGTGGTCTGTAACAAATCATATGACATTTTCCTTAYAAAATGTKA ATTGGCTAAAAAAAATAATAATTCATTTGTGTGATGTTTTGCAGCCTACT TTTATGCTGATTTGTCACATATATAGATAACCTAAATAATGGGTTTGTAA AAATACTCTTTATTAATTCCTGAGAAGCAAGAATTGACAACCTTGTGGCC TTTTTTTTCTTTTTACTATTAGCTAAGATAAAAGTAAGGAAAGATTTTTA GAAGACAATATTCTATTTAGTTTTACAATGTTTACCATTGCATCATAAAA ACTAACCAATAGAAAAAATACAAAATTGCGAAAAATTGATTTAAATACAC CAACAGTCAAAATGGATAAAGAAAACTTATGAAAAGTTCACTATATACGG GTTTTTCTTATATCCGAAGTATAAAAAAATTACTTTTTTTGTATATTTAT GTAAATAAATAATTTTGAAGCTCCCATTTCGAGGCAACCAGTGTTTGATT AGATCCTGTTTTGACAAACACAGTCGATGATTTTTAGTATATCTTTCAGA AAAAGTGTATTTCTGGGTGCCCTCATAATATATATGCGTATTGATTAAAA AACAATATGAAATGAATTCAAAAATGACATATTGAACATATTGAAGGGCC AGCTTACGATTTGCACCAATAAAGAGGAAGAACCCTCGAAGAATTGTCCT ATGAAGTTGATACCAAAAAAAGGAAAAAAAAGTTGACTATATTCGGATTT TTACTCATTGTTTCTTTTATGCGAAGTATTCTATTTCATAAAAATTTGTA TCTGAGAAAACGTAGACTACAAGCGTATTTTTATTATTTCCGGATTTATA ATTAAATATTTCATTAACATACTTCGATCAATTATATCAAATTTATAGAA TGAGATTATAATATTATATTATGTTATATATATATAACTATATGTATTTA ATTTTTGATATTTTATTTGGCCTTGATATGAAATAGCTTTCAAATAGATT ATATTTGAATAAACTAATTTGCTTATGAAAAGGCATTTTTTCTACACATA AGGGTTGTTTGAAGTGCCTCCAAAGGATGGAGCTGATTTTTTTATAAGGA AAGATAGAGGAAAATTAGTTACAAAATGTAACTAATGCTCATTTTGGGAA AAAAATATTTTAACTTGCTTTATTGTTGACGGCCTAATAATCATAACAAT TTTTTTTTTTCACTTAAAATGAAAAAAAAAAAAATAGAAGTTTATTTACT GTGATGCGGTTTTTTCATAACAATTGAATGTATTTTTCATTATTAGAGTA ATTTTCAAAAATTATCGGTCCATTGATTTTTGTTAATTTTTATTCATAAG CAATAACCTCATTGTTTATAATATATATTTCTTTTGTTGTGTATATTATT TGCTTTAGTTATAAAAATAAATGGAAAATTTCTAATTTTTGATTACTTTT TGCAAAAATGTAATATAATGTTAAACTAGTCATTGTCTTTTATAATTTAA AATCTCAATTTCAAAAATGATACTTATTAGGTCAATTTATATATTATAAT AAAATATATACGTTAAATTAAATAGCAAATATTTAAAATAAGTTAAAGAT TAGATTGGAGAATAAACAAAATATTTTATATATTTAAATGTAGAAACTTT TATTTGAAGTAAATGAAATAAGAGCTTTTTGAGTAACTCAAGATCCGTGA TCGGAATTATAGGGTTCATTGCCCCCCCCCCCCTTCCACTTTTTGGGGAT ATGTTATAGTTATCAAAATTTAAAAAATTCCTCTTGTCTAGCAAATTTTG AATATCGTAATTTTCAAGAAAATTAAATCAATATATTATTTTGAAAAAAA TACATGCTATTTGTTAAAAGTTAGTCTCTTCTGAAAATTTTATATTCTCA TATTAAAAAAGGAGTTGTTTGGTTCATTAAAAAAAAAACTATTTGTCCAA ACCTGTGGATYTCCCTGATGACGCAACCATGACATAATTTGACCCTCCCC CCCCCCCCCACTTTAGAATTACGGMGCCGCCATTCACTACCAATTGTATT CCCATCCCCATGCTATAAATTAATGTCGAAACAAAAGCTACATGGTCAAT ATAACTCAGGGCTGCATGAATTGATCATTTAAATTTAAGTAACATGGTCA ATTATGTTAAGAGAAGACAAAATTGTCTACGTCATGACAGAAAATGATAA AGTAGCGTCAAATGTTGTATCATTACATACGACATCTTAAAGGAGATAAA TATTTTTAGGTAGCTAAGAAATATCCTAAAGCTTTGAGGGACGTATCTCC ACTCTGTTTCTATCTTTGAGAAATCCTAGGGCTATAAATTACTATATAAT ATATATACTATATAAATCTACTAGTTTCGATAAAGTTCTATCATTTTGTA CTTAACTGAATATTTCTTATAGCTAAATTATACATAATTATTTACGCCAG TATATATAGCTCACACTTTAACAAAGAGTACCCTAGGATGCATATATATC TGAAAAGAATTGACAGTGCATATTAATAAGTAAATAGAGTTCGAAAAATG GTCCTTGAAATCTGAAAACTAATTATTTCAATAACAAATGAAGATTGGGT GATTGAAATTAATTCATATTTCGCAATATTGAAGCATAAACAATTAGTTA CAAAATAAAACAGATTTGAGCTCTCCATTTTAAGTCCAAGTTGAGAAAAT GACACTTGAACGAGAAAGACGAATTTCAATTCGTGCAATCCTTGACGTGG GAAAAACCACGACGGAAGAGTTGTTGGTCGTCTCCAGGATSAACTTCTAC MCTGTCAGCAAGTCTGAAAAGTTGGAGAGGAAGAATGGCTCTATCAAAAA GACCAAACTAGATCCTAGAAGTTAAAGGAAATAATCCCATCAAGTCTTTG AGGGTCCATGCAAGAGATCTTGGGGTTTCACACCAGAGTCTTGAGAGATA TCAAAAAAGCGGGGGGAAATAGCCTTGTGAGGATTGAGAGGCAACTTTTG ATACCAGCTATGAAAGAAATTATCTCCTCTGTTGCAGGACTACATCTGCG GATGATGACAGGGCCTCCTCCCGCCGCCTGGAAGCCATCATTGCTGATAA TGGCAGCTACATTCATTATTAAGAGAGCTCAGACTCACATCTATTTTTAG TATTAATATAATTTTGTTATAATTATATTGTTCATTAATAAGTTATACTT TATTGAAGTTTAAAATTCAAAGGGTTAAGATTTTAATGGACCACTTGGTA CATGATCTATAAGAACGAATCCAAATATTCATATAGCCATAAATATATCC CAAAATTGTCTTGAATTTTATATGATATTTTGAAATGGAGTCCAATCTAT TAAATGTGGAGCATATGTATATATAAACACGATATGATTACATATCGAAC AAAGTYCACATATATTTATGTAAAACTAAATATGATTACAATTATTTTAT ATACTTTTCCAATACATTTGTGTGTAGTATAAATATACATATAGCAAATA CCTATGTATATTCATAACAAGAAGAATTTGATAATTTTATCAGTTGTTTT ATTTTAAATTGCTTCAATTTTCTATGTATGGCTATACTGATATAGATATG CTTTTTTGTTATTATTCTTTATCCTCTAAAAATGATCACGATCAAAGGAT ATATATACAATACATACATTATACATATATGAAAGCATATAGAATCAGGA ATAACAATTGGTAAATTTTAACAATTTCCTTTTTACTTTTTTTTTCTTTT CTTTTTTTTTGTGGGGAGATGAAGGGGAACTTTTTTGAAAAAACTTGTTT TAAATCCTTTTTGTGATCAATATTATAACCTCAACGAAGAAAAGAGACCA CGTCGTTACCTTCATTGTATTATGCAAACTTTCCATAGAAAAGAAACAGA AAAACACTATAAAATCAGTTTATAGTTACCCTAACACTTCCCAACTAAGG AAATATTACTCCATAATTAAGTGGAATAATTCATAGTTAATTCAATCAAT CAAAGTTCAGAACATTGATAAGAGAAAATGAAGCATAAACAATGAGATAA TGGGTCAATTTTAGAAGGGGCAAATGAAAAATATGCTACAAAGAACTTTG CACATTAATTAAAATCATCAACACGCCCTTACCTTTATTGTATCTCGCAA CCTTTTCTACAAATAAAGAAACAGAAAAAAGGCCTAAAATCAGTTGGTAG TCTATTCTTTCCAACGCACGTATTCAATATAATTATTCGGAATGGTACAC AGCTTAACAAGAGAAAATTTTAAGTGGGAAAATCAATGTTTAGAATACTG ATTAGATGAAATTAAACAGAAAAATCGGCAGGTTACATCAAAATGCAGTG TATATTTTTGTTAGTGAGGTAATCCCGAAAGACAAATCAAGTCAAGACGC TATCATACTAAGTCTGAAAAATAATGTCAACTGTTTTAGTTTATAATTAT GAATATTCTGAAAAGTGACTGCTCCAACTCATTTTGGTTCTTATTCTGTT GTGAAAATACCAAACTATTGTTGTATGAAGAGTAGTCCCACAGTTGATAA AAATTGGCTACCAACAATTTATTTGATCAACTTAATTATTCCTATTGATA AATATAAGCACAAGTATAGATGTGAAAATTTGCACACTTTCGCTTTTCTA GTAATAAGTAATTCATGTTCTCTGTTATTATTTGTGACGTCATTGCACCA AAGAAATATGTATACTATTACATTAATTAAATTTAAAAGTTAGGGTTTAT TTCTTATTCGGTTGTTGTTGTTAGCAAACCATTTATTTGCACTGGGTCAG GCTCTTTGTTAGCTTGAAACCATGAGCGTAAATAATTTATAAGCAACAGG TGTTAAAGAATATTTTGTGTAATAATTGTAAAAACAACATTAATCATATT AACTTACATTTGTTACTCCATAGATAAACCCATCTTTGACATCGATCCTA CAGATACAATTTTCTCTGTGGAAGAGGGAAGTGATCTTGTTGTGAATATC AGCGTTCGTGCAAATCCATTTGATGTTACCTACAGATGGGCAAACCCGTA TAATGAAAATATTCCTAATATTACCCTAGACTCAGACAATATTCCTCGGA TTTACTCTATTCAGAACCTTCTCAATATCACAGAAGCAAGAAAGGAAGAT GCAGGACGCTACAAATTTAAAGTAAACAATTCTGAAGGAAAATCCAGGCT CAAAATTAAGATCGATGTTCTTTATGGAGCTACGTATGTTTAAAATTATA AAAAAAGACTCATCATTCGCTTTTTCCTTTTTTTGAGCAGGATATCAGAT ATTACTGACGCAGTTATGGTCGTAACAGGGGAGACCGCTCATTTTGAATG TGTGGTAGATGCAAAACCCATAACTGCTGATACAATTAGCTGGTCCAGAG TAGGATTCGATATGGAAGGACGTACATCCATGCAAAATGGAACCAATTCT ATGCATTTAACTGTGTATAATGTGAGCAAAGAAGACGCAGGAGAATTCCT TTGTACTGCCAATAATGGGATTGCAGATATGAAGGATACAAAATCTACTT TTCTTTTAGTGAGACGTAAGTGCATGATCGATCGAAGCTTTTACACACAA ATTGTAATATTATTTTATAAATACTTAAGAACGTCCAAGGATTGAAGAAT CTCCAGCCAATTCTAAGGCTGCCTCAGATAAACGGAAAACAGCCGTACTC ACTTGTCGAGCTCAAGGAACTCCTGATGTGGTTTTCTCATGGAGTCGTAT GGGATCCGTTATAAGTCAAGAGCCTGAAGAGGATGAAAGTGAAGAAATAG AAGATGAAAAATATGAAATCAAATACAATGTAATCGATAGGTGAGTTCAT AGTTTTAATGGAAGGMGTTAATTTATTAAATTTCATGATATTACCAGAAT GACATGGGAGTCAGAGTTACACATCAAAAACGTTTCTTCACGGGACTATG GGTCATATGACTGTATAGCCCAAAATGAAGAGGGAATTTCGCGATACGCA GTCTACTTAAACGTGACGAGTCGKCCAGACACACCAACCCTACTCAATGT TTTTAACTATACATACAACTCTGTGTACCTAACATGGATCCCAGGCTTTG ATGGAGGTCACAAACAATCTTTTATCATTCGGTATTAATAATATGACGAA CACAATTATAGAACTTTTAGCAATAATAATCATTCCATTTATTCTATTAA CGACACAGATATAGAAAGAAAGGTTCAAAGACCTATCAGTATGTAGATGT ACATCATTCCTCCACAAATTCATTCGAAGTAGAAGACCTCAAGCTTGGAA CAAAATATAGCTTCTCAGTCAAGGCTTTCAATGATCTCGGAGACTCGGAA TTTACATCGGAGAACGTTGCTGTAGAAACATTAAGTAAGATATAAGAATT ACATGTGAAATGCATCTATATGAGTTTCCTCGTGGTATCTGAAGATCCCA TTTTTACAGGGTTTTACACCAACGAAAAAATCCCCAAATAACAAAATTTC TGCATAAACCTTGCATTTCATTGAATATTTCATAATATAAGCTTCCTTGA CGTCAAGAGTYTCAAACATACGGTCCGAGGAGGTTTTAGTATGGGCTGGG AGATAAATCTTCATTATTAAAATAAATCGATGAATTTCCTTTATTATCTG TTATTACTATATTGTCCGCTAGAGACGCAGTGTTTTAGTAATCGCAGACT ACATGAAAGCAACACTGTGGCGGAACTAACACTTAATAAGTGTTTTCATT TGATTATGTGTAGCATCTGTTGGTGGCGTATTATTAATGCTTCCTTTAGC CTTTCACTCTCATCAGCGAAATGTCTTTGTCAAAAAGGATAAAAGTGGAC GAAGAGAGTAGGATTTTTTCAGAATAAAATATATATATTATATGTTCGCA AAATGAACATAAAATCCGTGTTCTTGGTATACCACCAGTAATTTTCTTGT TCGTAGAGTACAAGAATGGCCCCATTATGAAACTCAGTATAGAGACAAAT ATAACAACTTGAAGGGAGAACTGAGAAAACAAATTACAGTGGAAGTCATT CATTTGTTTAAAATGCTTTCCATATGTATAAAAAACAGATTTTTAAAGTT CCARAATGCAGTGCCCAGATGTTTTGCAACTTTGTTAATACCACTGGTGT CAGTTATACATAACTTAAACAGAAACGCAGAGTGAATTCACTTTTTGTAT TTTCTGTTGCAATTGAGTAAAATTGCTGTTAAAATGGGGCATACTTTTTG AGTCCTTAGGTTGTTCATTATGAATTTAATAAAGAAAAATTCATAGAATA AGGATTTTSATAAACTATTTATTTTTCATTACACTAATTATTTTTATTGG TGACTAATGCAAAGAAGACCAGTAGTTTTACTGTCAGTTTTATGTAATTT TTCAATAATTTTACTGCCCAGCCCGCTTAAGCTCCAATTAAAATGCATTC GGCCTCCAAACCGAATGATTTTGACACCCCAGCTTTATGTAATACATTTG ATAAAAGTTGAAAACAATATATTGAATATTCACTTAAAATAAAGTATGGA ACAATTTATTTTACAGTGTAAATGATGTATATATCTATAACAAATCAATA TTTCATCATGATTCCACATATTAACGTATGAAACATGAATTAATTTTGGA CTTTTTGTAACTACCATAAAATAAATTGGTTCTTCTTAAAATTAACTTTC TTATTCAAGGTCAATTTTGGAAGGGTTATAGGGAAAATTTTCCGAGGGCC TACTTTTGAATGCAGCTCCACGTTGTTTTTTTTTAAATAGTTATATATAT AGATAGTATACTGAAAAGAAATTATTTAATAAAATAAAAATGATTGACTT TCCAATCATTAAGGTCATTTTGAAAATAATTAAATTGAAAAATTGAATTA TAAAAAACAAATGTTATTAGAAAAAATATGCATATAATATTTATAATATA ATAATTTGAGAAAAAATAGATATTTTCAAAAAAAAAAAAACGTTACTTAA AAAAAATTAAAGGAGTTTGCACTTTATTGAAGCCAATTTAATTAATAAAT TAAATGAAAGATTCTAAATTAATGTTATAAGAAATTAAAATAAACTAATG GAGAGAAAGAAAAAGGCCGAGATCTCTCTTACCGAAGGACAGTTTCATAG GAAATGAGAAACCCCAGTATCTATTTATACGTTGTGTACCAGTCGCGATG TGTATCTAAGTTGCAACTTGTATTAGCACATTGTACATGTTACAATTTCT TCTTCTTAAAACGTATTATTAAATTTCAACATTGGGCATTCCAATTGTCA ATTATTAATCGATACTATCATTTCATTTTAATCTATTAAAATTATATGAT TATTATGTATTTATGAGTAATATAACTGTGGGTATTTGGAAAATATAATT TATCAGAGAATACTTGTATTTTAGTAATTTGTATTAAATAGCCAAAAATG ACACTGCTAATATATGGATCATTCGTGGAATATTCAATTTTCGCATCATC CTCATCCTAAGTACTGTAAATCCTTCATTTAAAATTATTTCTCTTATTTT TTGGTTGCAGCTTGAAGAACTTGAGCTTAAACAAGGTACAACTTGTAAAA AATTGCAATTCTCTTGTACAAAACAAATATATAAAATAAACCTATTTATA TAACGAGTTTGACCATTTTTGGGATTAAACCACAAACCTACAATTGAATA TTTTTGATTAACATACCTACACAGAGAATGACTTCTTCTTTACACTGTAT TTAATCATAATATAAAAATACATGTGCCCTCAAAAAATGTTAAAACACAA ATCTACATACATATGCCTAAAAGCTATGACTCCTTGAGAAATGTGCATTA TTCAGGGATCAAAATATATAATGTTTACATATTATATTTTTCAATTCCTC ACATTGAAAAAAAAGAGAAATGAATATGTATAATATATTATTGTGTTGCT TTAGGGTTTTCAAAGATGTCTCTTTTGATAAATACTACGGAAATGGTTCA AATTTAGGTTGATATGTTTACTAATAAACAACTCTTCACTTTTTGGGATA AATTATTACTTGATAAGTTATTTATGGAGAAGGGGTTTTAATTCCATCAC AAAACCTTTAATAACTTTATGATCTACCACAGAATACTAATATTTTAGTC ATTTGAATTAAAATAGTCAAAAATGARACCACTAATTTATGGATCATATA TGGAAAATTCAATTATCGCATTATCTTTATGCTTCATTTCAAATTATTGA AATTATAAACATATATAAACAATTCAGCACTAAACTGTAACCCATTCATA CATGGTTTCTCATTTCCCGTAAATTGTCTTTCAGTGGTATCCCAGGAAGT ATTTTTTTAAATATAAGGATCCCCTTAGTAAATATAAATACTGTTAAATT TAATTAATTTTTTCGATATTTTAAGAAGAAATGGTAAATTGTAAGCTAGT CTTACCAAATGCATTTACAATTCTTAATTTTTTTTGTTACCACTTTTTTG AATTTTCCGTAAAAAAAATAAAAAAACGCGCTCAGTAATCGTTCGCACTT AGGGGAAAAAATATACATTATGTATTGTACATGCACATATATAAAACCCA ACCCGTGGGCTAAAACTTAACTCGTGCATTTCTAAAATCCACATATCTCT CTTTATAACAGTATGAGACATCTGAAATGTCACAGAAGCTTTTATTATCA AAAAAGTCAAACTAGCAATTTTCCCTTGGGGATAAAACCCCAAATTTTCC GGGAAACAGAATCCCGGAAAACAACCCCTAACTATTGTTTAAATATAAGT ATACTTAGTTGTAATACGTATATGTGGGTATGGACCCTTTCTCAGGTGCT TGAAATGAGTGTTCCTAAATTAGGAATATAAATACAATCCACGAACTATA TCATAGAAAATGGTTGTTTTATTGTTATTATAATAATAGGAAGAAGAATT TACGGTAGGGAAATACATATTACTAGATAGGAAGAAAGACAATTTTAAAG GAACCAGGATAAAAAAGGATTTAACGAAATGCGTCTATAGATAAATTAAA TAATGATAGTAACAATAATAAAAATAATGAAAAAACATTAAAAAAATTAA ATGCAGATGTAGATATGTAGGTAGCTGTATCCGTTTACATACATACACAG ACATAGGTAAGGAAATACAAACATGTATACCCAACATTAAACCGGTTTGC TTTCAATTTTTTTCGAATTTCAATTACGGCTAYNTCGAAAAAGTTGGGAT TTAGGAAGGATATAACCTATGTAAAATTTTCATTTTCCTACGACATCATC TTTAAGTATCACATCTAATTCAAAGTTTGAAAGAGGGGCAAAAGTTTTTA ACTCCGTCAATGAAAATTAAAGTACAACATTAATACATATTTTACATTAA TTAATAACGATAGATATTATTCTAACCTGTAAAAAAGATCTATAACGCTT TTTACTGAAAGCTACCCTATAAAGCAAAAATAGAGAATAACATTTATTTT ATTTCCTATAAGAAAAATTAGATTTTACTCAATAAAACAATTTTAGAAAA ATGTCTATCAAAACTATAATACAAATTAAATAAAAATGTTCTCTTAGCAT TAGTAAGAACAGTGAAAAGAAAAAAAATACTTTGAATGAAACATAATCTC AATTTCTAAACAAAAACTTAAAAATCTCAGAACGGGACCAAGTTATATTA TTTACAAACTCCTAACCCTTACTGAATTAGTCTCAGACTGTACTCCAAAA ATGTTGTCGGTCTCTCATCGAAATGAGAAATTCTAATATGTTATATCATG TCAATCCGATTTTTCCTCGTTGAAATATATTAAGATTAATTAATTTGAAT AAAAAATAATTCTCAATCTCTAACCAAAAATTCACAAATCTTCGATGTAA ACTAATTTTTTTTTTTTAAATATGCTTGTTCTTAGAAAATGGACTATGAT TCTGACTTAATATACATAATAGGCTTAAGCAAACCTTTGATATTAGTTCG AGAAACAGTATCAAACCGTATAAAGTTGATCCTTTACAACTCTTGACTCG TATTTAAACGATCTCAGACTGTAGACCAAAAATTTTATCGGTCTCTCATT GAGACTCTACAACTTTTTATGACGTCATCTAACCAATAATTCTTTATATG AAATTACAAATAATGTTATCGACAGTTCATTAAGACTAATTTTTTGATGA AACTACTCAACATTAAGTATTTTGCTGGATTGGTCCAAATAACATATTAA ATATGTCACTTTGACCTGTTCATAATTTTATAATCAAATTGTATACTTCT AAATAAAAATGCATTACTCAAAAATCACCAAATCATAAAAATATATTCGT ATTTTTTAAGGATTAATTAATTTATCAGGGGAGGGAATTTAGTTCTTTAA TTTACTATGAGATATTTATTGCTTCTTATTTAATATAGACTTACTAATAA TATAATTTGCAGAATAGAACAAAAAAAATTGGATGCTTTTATTATTATGA TATCTTCTTTACCCCATAAATTGTTGCAAGAAAAGCAATGTCTTTAGCAG AAATGTAAGAAGAAATGAAAGTCATTTGAAATTATAAAAAGTTGGCAAAA TAGTTTATATATTTACTACATATATGTAGGATAATATGTATCATGTAAAA GTAAGGGCATCGGTAGCTATTTACTTAGAAGAAAATNGATTATTTTATCA GAGTAACTAACYTTTGTGGTATATTTTTGTAAATAGGTATCTTTAGATAT ATGTACACCTATATATTATAAGTAAAAAATCCCAGTTAAATTTGACATTT ACTATTATCTTGAATATATATATATTATTGGTTTTTAAAGTTGGTTTTTT TTTACCTTTTTTTTTTTTTTCATTTTCCCCGTTCCTTCATTCTTTGATTA ATGATATTTTATTTTACATATAATGAACATTATAAGTTCACCGATACAAG GGTACCAAATATTTTTAGTAATGATGTGTTTACTTATTCTATTTTCAGGT TACCTGGAAGGTATGGTAAAGCCGGAGGATAAGGATGTAGAGAGAACGAT ATCCCAAGGTATCATACTCACGATCACATTGGTATCTGGTCTTCTCCTTA TCCTCAATGTCATTCTCATATCCTGCTACGTTAAACGCCGTCGTATAATT CGAATGAATGGCTCTAGCGTAGCCGAGGGTAAGTGAAACAGAACCTTTCT TACTAATTCCCCATTTTTTATAGAAGGAACGTCCTCCTCGTCCTATAGTA GGAGTAGTAGGGATCGTACTCAACCAGAGGATGAAATGGAAGAGGTCCCA TTAATTGTTGTTGTTCCTTTGTCCATCGTCGCAACGTTTTTGTTTTGCAT GGTCGTCCTATTCCTATTTTGTGTTCATAAAAATAAGTTCCTCTTCAAAT ATAAGACTGAATCAAAGGCTGCATCCTCAGAGGATGCTCAATCCGGAGGG GAGGGTAAAGAAAAAAAAAATCAAATACCTTTTGATAAGAACCATTTTTA CCTTAACAAATGATTATCATAACTAGAGATGTGAAAAACATTGCAATAAA GCGTCCACCCTATTATATTTTATTGGAAGGMGGTTGGTTTTTGGAATTTT TGGAAAAAAAATCCAAAAGTTATTTTTTTNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNAAAACCTATTTGCCTTCACAGAAAATTAAATTTT TAAGAAAAAAATTTAAATTAAATTATAATCATACATTAACTTTTATGAAA AAAAAATATTAAAATTTCAGTAAAACGCAAAAATTCCTCGGTGGGGGGCC CCCCCCMCCGAGGAATTTTTAGCCCCTCCCTCCCMCACCCTGTGAACTCC CCTGAGAGATCACTATAAATTGTAGTACTCCTTACTTACTAATAACTTTT CATACGTTTGAATTTTCAAAAGAATGAGGAATCATTCACAAGATTTACTT TTTTGTAATTTACGCTCGCCGGGAAAACTACATTTTTCATATCTCTAATC ATAACACGGGCTGGCACGTAGAGCATTTTGAAGTGACATCACAATGAACA GAAAACGCTATTACATTGGATGAAGATATTTACTATCAATAGTCATTACA TGATGTACATTTCTTTAATTTAAGAGTTTAAGAGTTTTTTCATTTGTGGT GTCACGCCCAAATGGTCTATACATATGGGATAACGGTGGATGGATGACGT ATGATTTATTTTATCCAATCAGTTTTTAACTATATGTACAATACACTTTG GGTTCACCAAGGTTAATAGAAATACAAGGTTATACCATAACACGTCTACG ACTGATGTTTGACTTCCACCACGCTTTTAAATATTGACGACATAGTATAT TAGTGGTTGCGTGTTTTCTCTAAATCTGTTGTTCTTGCCCAGCAGTCGTT ACAATGCATTAAATTGAAAATTATAGAAAAAATTACGTAATAGACTATGA KCGGTTRCGTGTTTTCTCAAAATCTGCCTATCTTGGCAAGCAGTCGGTAT GATACATCAAATTGAACATTCTATCAAGCCCGATTACATAATGGTCTAAC CTTGTATTTCTATTAACCTTGGGGTTTACTAATATTTTAAAAAGGACAGG AAAGGAACGAATAAATATATATATATGTATATAACTAACCTTCATCAATA ATAAACGTATGTTACTCAGATATGTAATCCTTGCATGATGGAGAGACTTA AACATATATCAATTTAATGAAGAAAAAGAGAGGGAAAAAACTATTGAGTA GTCCTTGTTTCTTTATCTCCTTCATTGTTTTTTTTTGTTTTTTTGTTCTT TATAGTTCAAGGTATATAACTTATACATAGGCTATTTGAACATACTCTCT ATGTAAACTTCTGAAGAAAAGAGAAGAGAAGGAAAAGGAAAGATTCAATA TTTTTAATGAAAGGTGGGGGGGGGGGGGGGATCCTCCAAAACACGCATGT TGTAAGAATACTTTCACTACGGTAAAAAATGCTCATACATACAATAATAG TGGATAGTTGGTTAAGTATTATTTAAAAAAAATAATATTATGTACTTTGT ACGTTGTGCATGCTATAATACAATGTGTTAAGGTTAAAGATGCTAGTAGC AAACATCCCTATTGTTACAATATAAACTTTATCAATTATGTAAATAAGGT AACGAAATGACGTCTACTTTTTTATTTATTTTTGTGTTCTTGTCTCTTAT AATATGTATATAGACATAGAATAAAACAGATGTTGTTGCATGTATACATA TGTATTTAGAAATATTATAGTTGTACTCTGAAGAGAGTTAGCAATAGTTG TACAATACTGTGTGCGGGCCCTTTGCCGAATTGGTCATATTACCGAACGG ACACTATGCCAAAAAAGTTATCAATATATTTATTGTTGAGTCATAGTGTT GTATCTATCCTTATTTTGGACTAAACACTGAAGTCAATTTTAGTTCAGTC TCGGTTTGGTCGACTTAAGTGCTGCACATTAGTCCTAAAAAAGATCATRT TCGGTCTTAGAAGACGTCATTCAGCTTTATTTATTCTTTGTCTCATCAGT TTTAGAACTAATAGCCCTAAGAACCGGTCCCAAAAGGCTTATAGAGCCTG GACTAAGCTGGAACCATAAAACACTAATGATTTATCCAACAATTCTATGT AATTATGATTCCGAAATATGTTAAACCAATTTTAAAAGATATAATATTTC ATTATTGGTCAAGAATTGCAATTATAAAGTGTTGATTAAATAATATTTCA TTATACAGATGCTTCAACCATTGGTTTGAACCGCTATTAAAACCGATGTA ACACAAATCATGACAATCACCCAATGAATTAGAAATTAGATAGAGAAAAA ATGTTTAAATATGAGAAAGAGTCAATTAATCAATGATTGAGACTCAAGGG GCAATGCGGATGAATCAATCGATTGTATCAAATGTTTTATAGCTTTTATG TAGATTGTCAAATCTTGATCGACATTCCTGTATCCTGAGGTCTTCTCTGA CTCTATAGTTATCAAGATGTGTGTTTTTCGTAGGTTTAGTCTGGTAGATA AGTACATAGTATGGCAGCATGAATTATCATAGAGTATTTTTAATATTTTG TTCGAGAAAGTGTCTGTCAGGCAAAATGTCATAGAACCGTACAATATAAC TACATGGTTCCATTTTATAAAGGATTATTTTAGGAAAGCACAAATTCTCA TAATTAATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNACAACTCATTTTTCGGAATCAACATA AACATTATATGCGGGGAGTTAACACAAAAACAGTATTGAACAAAAATAAA GAAAAGGGTACTATTTTTTTTTCYTTWACATTATAAAAGCATACATATAG CCCAATATTTGATGAGCATAATTTGATCTGTTATGGGGGTTGTATTCAAA TTTATGGGCTGAGATGAGATACCCAAGAATTTTAACTATATTAAAACCTT TATTTGATTTATGAAAATTTATTTGCCCCCTAATTGGTTTTTTAAATAAA GTTTATCATTTTCTAATTCGTTTAATGAGATAATGATTTTTTTTTGATGA TTTAAGTAGAATATAAAGATTAAAGAGAATAATTTGTAAATTAAAAATTA TTTTTTTAAAAAATCATTCAGGTGGCTCAGTCTAATTTAAAGCAGATGAC ACTATCCATTACGGAAATTATAAAGGTATTCACTACATACATATAGGTAC ATATTCACTTCCCCGAAAATGTCTTAGTATGTCAAAAGTTATAAAAAGTG TTTGTCATTTTTTTCATTTTCTTTTTGAAAATTGAGGTTTTGAGTTGGCA AAAAAGCTATTCGTGTAAAAAACAAAAATAATATAACCCCATAAAAAACC CTGCTACAGCATAACTTATTTTTAAATACTAAGGAAGAAGCTTTTTTGAT AATATTTTGAAAAATTAAGTATATATCATGCAACTACAAATAATTAGTTT TCTTCTGATATTTTAAAAACATTTGAAAAAATAGTTGTTTTATTTATTGA TGACCGAAAAGTCATCCTGGAATTTTATGCAAATTTGTAATTTTGGACAA CAAATAATTCGATAATACTCAAAAAATCTCGAATATTATTACATTTAAAA GTGATTGCATATGACGTAAATTAACTAATAATCCCTACCTTAAATAATGC AGATCTACGAATCAAAAAGATTAATTAAATGYAGATCCCTCATCTATCAT TTAGGGTTAGAGCCGGTCTCAGATACACAGTCAAAGAAGCATGTGCAATG TTAATATTCCAAGAGGAGCCTGGATACTCTCCCTTCTCTAAAAAAGTTTT TTAAGGATGTAACTCCTACATGCAAATTCATAACGAGTGGATTAAGATGG AGAGAGAGAGGTCGAGTGACCTCCACCCTCATATTAACCTTCTGTCTATT GCACATTTTTTTAAATATATAATAGATGTAATTTCAAAGTAGTTTTGTAT TCCATTAGGTGCACTTTTATGCATTAAAAATCAAACTTTCCTATTATGCT GATAAAATAAGTTAATCTTTGAGCTACTTCATTCTTAATAGGTTATTCTA TATTTTCTTGATTTTTTCTTTTTAACAAAAAATATTAAAAAAATCATTTT GCATTTACATATGTATGTATGAACATAAAAAACGAAGAAATGCACAGATT TATTTATTTTATTTTTTGCAAAAAAAAAAAACAATAATGGATGCAGTTAC TTAGAAGTTGAGGAGTTTTAATAANTTTAATAATATTCATAATATCATAA ACAGTGTAGAATTAATACATGTATTTTTACAAATGAAAAAAAAATATATA GCCTGTCAATATAGTAAAAAAAAAAAAGCATAATACTATGTTTTTTGTTT TTTATAGTACATACTTCATGATAAGTAATGACCCATGGGATGACGTACAT TCAGCTAAAAGGGTGATTTATACAATATTATGAATATCGATGAAAGTTAA AGAGGATCAAAGAATAGTTGTTGTTCTTAAAATTCTTAGAAAGTGAAATT GGTTTAATCTTTTATTATATATATATATAATATCAACTTTAATATAAAAA CTAAAAAGAAAAGAAAAGCTAATTCATTTCCTTTTAAAACACTTTGAAAA GTTTTTAAGATGAAGGAGAAAGAAAGTTGATTGAGTTTCGAATTTCTTCT CTGGTTTTTTAATTTCATATAATATACATACACACATTCACAAAAATATT AGCTTAAAGCCAAATGCTTTATTCAATCAAGTATAAATCTATGTGCATAT AAGTTATGCTTTTTGTACCATTTTGTGGGTTACAAATCTTTTGATACCAG GATTAGTTTTCTTACTTTTATTTTACTTGTAACTCTTTTCTTTTATTAGC AAAATACTTTATTGTTTTTTTATGTACCTTCAAAGTATAATTGTAAATTT GATTTGATTTGATCATTCATCATTGAGTTCGAACAAGCGATGAATGCATA CAAAATTCATGAAAATTACACAAAAAAGAACAATTTTTTCGTTATATCAA TGTGGCGGCGCAACAGGGAATGTTTGTTCCTCCGATGAAACAAATTTAAA GGKCCTTCACAAAKCCTCMTAGCCACTAAGAAGGGAGAGAATAAGACCTA AAACCACGTTTATTCTCATTGGGAGGAGCGGAGGAGGGGACTTAAAATAA TTTAGATCTGAATAATATGACTCTTTTTTTTCAGACAATTTCATAAATAG AAAGAAATATTTGTTTTCTAAAAAATAATGTCATTAGATGAATTTATTTC GAAAGGGTCACAAGAAGACCTTTAAGAAACTTTAGATTAGAAATAAAATG AAACATTATTTTTTTAAATAAATAAAAAATGACGTATCATATTACCTATT TAATAGTAAGTTCTGTCTTCCAAAAAATGGTCAAAAATATGCCTTGCTAT TCAGTTTCTAATTTTTAATACAATAATAATTTGAGGGTGAAAGGGTAATA AAAAAACTGGTGGTTCTAGGGGGGGGGAGGMAAAGAAAAGCGCCACGTGG TTGGGTTATGAATGACCCCTAATAGCAGTATAACATTTTTATTTGTAAAG GTGTAAAAATCAGCTAAAAAATAATTTTTGGTGATAAACTAATTATGTTG TGCACTGATAATGGAAGTCTATTTTTGGTATTATGATACATGTAATCCAG TCGAACAGATCATTTTTTAATTTATCAAGTAGACGTATAAAATAAACAAA CATGTTCCTATGATATTTGTTTGGGTCTCCTAGGTCATTTAAAGGCTGTT TTTGACGGACATAAAGCCTCCATTTTTTTCTACATCCTTCTCTATATCTC TTTTCTTTTACTTTCATTCTTTGTTTCTATTGAGCTTGTCAATTCGGGCK CCCTCTGCATTTTTTTTTTTTTTTGAAATATTTTATCGTTAACAAAACCA GTAATTAATTTACTTTCATTTAAAAGTTTAATTTTATTCCAAAGTTGGGC ATTGGTGGTTAAATGGTTAAGAGCCACTGGCATACATACATAGTATCAAA TGTAATTGTATGTAAAATTTATTATTACAAAATATCGAACTACTAACTTT CTTTTATTTGTTGAAAGATGTTCACTTTTAAAATATATGAACCAGAGCAA TTTTATATCTAAATATTTACCAATTTTTGTTTTAAATCAAATTATTCGGC AAGTAATATCCTTGATTCTTTTTACTGTATTATAATTGTAAAAACATATA TGTATACCATCATCCTATGTAATCCTAATAAATTGCAAAAAAAAATTCCT ACCCTGAAAATGGATTAAGAGAGTTAAATAAATTAATGAACTCTTTTGTA GTCCCACACACACATTTTGTATTTATAAGAAGAGTATCGGTAAATTCAAG GCATCTGTTTGCGTTTAGCATCTATGTACTTGTACTTGATACTATACAAT TTGGGGGAGATCCTTCAATTCCTTCCTCTTCTAAATACAAACATATTTAT TTTCATTCTCTGTCTGTTTTTGAATAAATCTATTCGGTGAAAAAATAAAT AGAATAAAGAGAATGTAATATTTGTATGTATGGTTACACACAAAGCAAAA TACTAATAATAATATTATAATAGAGGTCTGAATCTGATGGTTCAAAAGGG AAAATATTACAACTCTTAAAGTTCATTTATACATAGATGTACCTATTTCT TTTATAACTTTTGATATTTTATAATAATATTTTTTTATATAATTCAGAAT ATAAAGACGTCTTATCGTATACGGGAAAAAAATCCAAAAATTATACTGCG TACATACGATAGAGAATATATAGAGTGATTAAGTTATAGTTGAGGGTGCT TGGATGGMATGATTTCATGATAGTCAATATGCAAATTAAATATATGTTTT GAACAAGCTGCAATGTCTGTACAAGTTGCAGTTTTTTCTTCTCAAAATTC CATATTTAATTAAAAAAATTGGGCATTCTATTTACCAACTATTAGTTGAT ACTATCACTTAATTTGGATGAACAGAAATTTCATGGGTATTATGTATTTA TGAGTAGCGTAACTGTGAAAATTTGAACTTTCTACTGACGCAAAGAGTAT TTATATTTTAGTCATATGTGTTGTAATAGTCAAAAACTAAAACATTAATA CATCGATGATCCGTGGAGTATTAAATTATTGCATTATCCTTATCTCAAGT AGTGTAAATCCGTCATTTAAAATTATTCATCTCATTTTTTGGTTGCAAAT TGTATTAGTTAGATCGTAAAAATAAAATGAAGAAGGGAGAACATTTGGGA TGAGGACAATACACTAATAGGACATAAGCAAGCTAAAAATGTATTTTACC CACAAGTTGTATTGATATAATTCAAATTAGGAATACAAAAATACGTAAAC AATTACCTCTTTGACATTCACCAATAAATAATAAATTAAAAAGGGAAATC TTCCTCATAAGTTGGGAACAGAAATCTGGAGATTTTTTTTCCATTATCCA TACTTCCAACTCCATATTGAGAAGGAAGAGATTACATCCAGTGCTATGAT ACTATAGCAATTTCTTGCTCATGCGCTATTGTTTTTAGTTTGTGCATGCC CTTTTAGGTAGGTTTTTTTTTTTGTAATCACACTTCCCATAAATATACTC TGGCACTATTCATTATAAAGTTGAAATGACCACCCATGGATTCCTTGTAA ATACATAATAAATGACTAATTTCAATAGAAATAACAAAATGAGTTAATTA ATTAATAGTTGTTAATTAAAATTACTGATGATGCAATTAAATAAAACCTA ATTGCAGCATGTATAATCTTCATATATTAACTACAACTTGTACATCAAAA AGCTCCCACTGCAATAGCTGGAATGAAATCGTGTAACAACGGTGCCCCCA AGAATCTATAATTGTTTACTGTAATACTTACATAAGGTGTTGGAAGGAAT GATCATTTACTCACCATTTTCTAACACCTCCTATTAATTACTATTCCTTC TTCAATATGATTAGGTGGAAGTACAAATGGAAGCACAAAATCAGCTACTA TCGAAATGTATGCAGGTAGTAGCTACAATGAAACTATGTCAGGAGAGACC TTGAGCTCTATCTCCGAGAAGAGTGGAAGSTACACCTCTTCCTCTGTAGA TCCTATTCATGGACATCATCATCACATTCATCATCATCCTCATCCGCTTC CACATAATGTTCCTCCTTCTGGAGGAGCARCTACAGGAGCCCCCAAAAAT AATGGTGGCCATAATACCCATCATCATCACACTCTTCCCTCTTCCACAAG TCACAATCATCATCATCACCATCAATCCAATCGACGGAGCCATAGGCGTG AAGAGGAGTCTATGATTGATGTAAGTTTACTTCTTGTCCGTATGACTAGC GATTAACATAGAACAAGGACTTGAAGATCAACATAAAATACAGCCGCCTA AAAAATTATATTTATTTTCCATTGGTCTGAATGATTTTATATTGTTTTCT TTCCTTCCCTTTTATCTTTAGAAAGATTTAACCATAGAGATAAAAATATA GAGTGGTATTTTTGTAGAAGAAACATATCAGTTTTATTCCCCTCACGCTC TACAATCGGCATATCCCTAAACATTGTTATTGTAGGAATGGGTGAACATA ATTTAGTTACAATATTTTGTTTTGTTTCATTAATATTTCAACAGTCTTGT TAACGGATAACTTTTCTCCAGTCCTAGTTCACGATCAACCCGTTCCTGGG TAGAAGAAGTAGGCAAATTAGAGTAAACATTTAAAGTAACTGACTGTGAC TTAGACCTTCAAATTCCAGCGTTATGTACTGATTATATATTCCTTCATAT ATCTATTTATCTCACTCCTTTTAGAAAAGTGTTTGCTCCGATCATCGACA TCARCATCACCACCATCATCATCTTGATCAACCGACTGTCATGGGAGCTG CGAGTACATACCTAGTCGATAATCCTCCTTCAAGAGCATCCTCTAATCAT TTAAATTATGTGACGATGCCCAGAAGGGCTCCCCATCCTCGACAGTCATC TACKCCCTCTGAGCCTCTCATGGTGCCTCGTGAGTCTTCTATAGAGGACC AAGGAAGACAAGCCTACATTCAGTCCCTAGGTAAAAGTCTTTTTTATTTT CGTTTTCAACCATTTTACTTAATTAGTGTGAATTCCCACATCCCACAGAT ATCAAACGACAGAATTTATCCAATTTGGAAGGGGGCAATACAAAATTAAT TACCAAGAACTCTGCATATTAATTAGGATCTTCAAACCTCAGCAACTATT ATTTTCAAAATGAAAATGTATAATTACCGCGATATATTAATCATTCATAT AAAAAGTTAATTTCTAAAAGACGTCAGATATATTTTCCCTTTATAAAATT GACTTGGTCAATTAGAATTTGCTCATGTTAAGTGGTGAACAATTTTCTAC AATTATTGAATAATAATTTCAATGTTGAGAAGAATTGACAAACAACTAAA AAATAATTTTGAGCCTTTTTTCTATTTCTTTTCAAAGAAAAGGTTGCAAG ATGCAATAAAAGTAAGAGCATGTTAAAGATTTTAATTAATATGCAGAGTT CTTTGCAACATTTTGCTAATTAATCCCTTGCAAAATTGATCCATTGTGTC AATTTTGATGTGCAAATGCACTTTTATCTATGTTTGTAATATGCATTAAC CCATCTCCTTCTTTCTTCTTTCTTTTTTATTCTCTCTCTATTTCTATTGC TGCATTCAGATCTATCCGTTTCCAGTAAGGATGATGGCGGATTAGTATGG TTATGACAACCATAAATCCTGTCCCTCTGCTCTTTCCTTCATATCCATAA TAATCCTTATTTCATTTACATGTTTAACTCTTTTCTAACTTTGTTTCATC ATCCTCTATTGGACTTCTCCTCATTCTCCATGGGCTAGGTAAAGGTAGGT AAAGTAACAATCATTCATCTATTGATTGACTCAAACTTGTTAAATTACAT ACAAACCAAAGTGTACACAGAGTGGGGACTTCAAAACTTAGAGTAGCATG ACTAAATTATGAAAAACGAGACTTTTCTAGAATCAAGAAAAGACAACTCA AAACTAATTTGTGATATGTTTAAATACTATATTTAACCAGCTTTTTTTTA GTAAATATCTCCTCCTTTKCAGCCAATAATAGTTGTGACAAGCTGTAAAA ACAGATTGGCAGCATGATGAAAACTGTGCCCATGGCGTACCATGCAATCA GGGTAGCAGCTCGAAGATAAACCAAATTTGGATGATATATGTGACAGACA TTCTTTCATAAGGCACCTCAAACTGCAAAGTACAGGGTGTTGAGGTCATT GCTTGAGGAGTACCACACGGAGACAGGCAAAAAGTCAGTCTCCGTGTTAT TTCTACAAAAGTTTTGATTCTTCTTCTATGTTTTTGATGTTATAGGCAGT CCGGATGGAGCTACCAACGTTCTCTTCAGCGTTCTCGACACAAACACTGG GGCAGAGGAGATTGCGCACGCTTTGCCTTTTTTGTGTTATTACTCCGACA TTTTTATAGTTAGAGACATTGGATAAAAACAAAATTTCTTTATTTTTATT TAAACTGTCGTTTCGGCTCATAATAAAACCACATGAATTTAAATAGGTGG GATAAAATTGTAATTAAATGCCACTGTAAGTTTTAGGTCTCCACTCTGTA CATATGTAAAATTATTGTTTTGGTTTACAAAGTTATATAGTCCTTTCTAC ATAACATGTGTATTATAATAATCAAACTTTTCCTTTTTGATCTAAAAAGT TTTATTGCTATAAATGTATGGATATGGCCTAGAAGAACAAGTCTAACGGA GAAAAAAAGAGAAAATAAACATCCCTTTCTTATAAGAACAAGTTTCTTAA TAAATACATATAAATGTTATTTATGCTATACTAGGACCATTATAGTATAT ATAAAATCCACTTTCCTTTAGACCCGTCATTCAAGCATAACATGTGTTGT TCTTTTTAAAATCATTCCTTAAGACCTTTTGCTAATATGATGATCCATGT ACATATAAAGCTATAACTTAATGAGCAAACAATTACCACGTGATCCATTT TACCCAAATTCATTACGGCTAAAATAATATTTTTTTGTCTGTAAGAGGGC ACTGAAGATGTAGCAAATGACTCTATAACGAACAACGTAGCACGAGCGCT GAATTTTTTTTTAATATATCAAATATTTAAAAAAAATCAGAAATTGCATA CTTCATGAAGAAAAAGCCTAACGTAGCTAGAAAAATATCTTTATATTCAC AGCAGCGTTTTGTCACTCTGTTTTATAGTTTACATAGAAATATGGTTAAC CCCATTGAGGGTTTTTTTTTGCTTTTTTGGTTTTTTTTTGCGCAGAATGA ACTTTTCTGTTAAAATGTGCATAATTATTTATTCCAAAAGGCTACTATTG AGATTGTTTTTATATAAATACTCTAAGATGTATTAATGATTCTCGGTGAT TTTGAGTTTTTTATCATTGGGTGAGACTGGCAATTTTATTTACCATTTCT CCCTACCCGGGACCTTTGATTTTATAACTTGACATTAAGACTTTTAGAAA AATATTTTTACTAAGAAAAGCCTCATTTTGGATCACATTCTCCTGAAACG GCAGGCTATTTGGCTTGATGACCTGTCCTAAATTGATTAGATGCTTAAAT TTTAATGAAAACGCCGTTTTTACAGACAAACAGTTTTTTAATCAATTTTG AAATTAAGAATTATTCCAAATGGGTCAATTGTAAAATAGATTGATTACTT GGTTAACAATATATAATGTATAGCTAATTATGTTGTCTGAAAAAGTCTAC TCTGGAAATAGTGTAATAAAAATATTGTATAGTAAAAAATAATCAAACTC TCATTACTTTTGAAGTACTAGAAATTTCATCCTAAAATTTTACAAATCCG CATATCTAGTATAACTGTGTATTTTATATAAATTTGGTCAAATTGTAGAG GATGTGAGTAAAATTTATTTTTTTGTTCCTCTATTATATAGAATCACTTA AAAGCAATTTATAATAAGATTTAATTCAGAAAACTATTCACCCAGCAACA CCTGAACAGTGATAACAACACCCTCAAGTAAAACTTCTTCAACCTATAGA ACATGAGTGCATCTTCATATTTTATTTAAATGTTGAACACATTTATAAAT TTTTTCAATATTTTATTTTTCAAAATTAAATCTTGTTAGTACATATTTAT AATTCACTGAAATGGCAGTTGTATCTCATTTTTACTATATATATCTCTCT CATAGGTATTGATAATGATGGTGATGATGTTGTAGGAGTTGGAAGAGGCA TCTACGCCTCCACACTGGACATTGAATCTCCCATCAAAAAGACTTATAAC GGTTTATACGTTCCTCCAGCTGACTACGAGTACTCTGACTCGATTTCTCT TCCTCCACCTCCATCAAGGGACTCTGATTATACAAGACCTCATTTTGGAT CACTTAGGCATTATTCTCCAGAAACAGCAGGCCATTTGGTTTGATGATCT ATCCTTGATTGATTGAGTCTATATTTTTTAATGAAAATGTCCTTTTTTTA AATAAATATAAAACATAAGACAGTATCTTAATAACCAAAAAAAAACCCAC AAAAATAGAGCGAAAACATTATTTTGTTTATTTTTTATGAAAAAAGTACA AAAAATCAGATGAAACTGCCATTCAACTTAAATTCAACACACAAATTACT TAATTTATTTATTATCTAGAATTTTGCTTTTTTTTTTCTTCCTCCTTGTT TATATAATTGTTGACAGA
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