BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:fat-spondin "fat-spondin" species:7227 "Drosophila melanogaster" [GO:0005576 "extracellular region" evidence=ISS;IDA;NAS] [GO:0004867 "serine-type endopeptidase inhibitor activity" evidence=IEA] InterPro:IPR002223 Pfam:PF00014 PRINTS:PR00759 PROSITE:PS50279 SMART:SM00131 EMBL:AE013599 GO:GO:0005576 GO:GO:0010951 GO:GO:0004867 Gene3D:4.10.410.10 InterPro:IPR020901 SUPFAM:SSF57362 PROSITE:PS00280 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 GeneTree:ENSGT00740000115000 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 UniGene:Dm.7709 GeneID:36919 KEGG:dme:Dmel_CG6953 CTD:36919 FlyBase:FBgn0026721 ChiTaRS:fat-spondin GenomeRNAi:36919 NextBio:801032 OMA:IWSNETH EMBL:AF135119 EMBL:BT031193 RefSeq:NP_477435.1 STRING:7227.FBpp0086129 EnsemblMetazoa:FBtr0086975 UCSC:CG6953-RA InParanoid:Q7KN04 PRO:PR:Q7KN04 Uniprot:Q7KN04)

HSP 1 Score: 306.605 bits (784), Expect = 3.532e-92
Identity = 155/352 (44.03%), Postives = 209/352 (59.38%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCE---GSSGFYANAPGDFLPDD------------MCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEE 449
            FW     A+ G   +A+WGSP  LE+EL+A    +RT+IKA GLWYPN    T + FR D+K+  VSLVSM GPSPDW+VG+SGL+LC ++C+W E  +  L+PWDAG DSGISY SP+S T+P   + RI++ +P DPR PFY+     M PLAKL L R+++  ++C DE          F   +  E     EE ++   C+V ++S WS CSV+CGKG+ MR+R +L P  A    C RQLV KEMC + +P+C      S    +  G+ L +             +C TS W+ WSECS +CG G   RTR F N +GRK+CPHI  +EK  C     DC+ +  E
Sbjct:  233 FWGENHIATAGFRSLAEWGSPAALETELRANGPKLRTLIKAAGLWYPNVNQNTSSKFRVDRKHPKVSLVSMFGPSPDWVVGISGLDLCTEDCSWKESMDFDLFPWDAGTDSGISYMSPNSETQPPERMYRITTMYPEDPRAPFYNPKSREMTPLAKLYLRREKIVSRNCDDE----------FLQALQLEVSDDAEEQDTRAECRVGDYSAWSPCSVSCGKGIRMRSRQYLYPAAADQNKCARQLVAKEMCVAAIPECADGPAQSKDRDDDEGENLANSQSLVGSNGEGAGLCKTSPWSVWSECSASCGIGITMRTRTFVNHLGRKRCPHITIVEKNKCM--RPDCTYEQVE 572          

HSP 2 Score: 90.5077 bits (223), Expect = 1.523e-17
Identity = 68/258 (26.36%), Postives = 108/258 (41.86%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDR----QLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMG--RKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGK 567
            C+ S WS WS CS +CG G++MRTR F+     + +G  R     +V+K  C    P C      Y       LPD  C TS+W+ WS CS+TCG+G   RTR      G  ++ C     + +     N  DC ++ E+   IC    +      +++      Q    Q             +   +  N   +    +++  +        C ++ WS WS C+ SCG G     R ++ +  + G+  SK+  K
Sbjct:  515 CKTSPWSVWSECSASCGIGITMRTRTFV-----NHLGRKRCPHITIVEKNKCMR--PDCT-----YEQVE---LPDPQCPTSQWSDWSPCSSTCGRGVTIRTRLLLLENGPDKESCTQRMELHQQKECVNPIDCHINAEQAKDICVQAPDPGPCRGTYMRYAYDPQ---NQHCYSFTYGGCRGNRNNFLTENDCLNTCNVLRSPYSSRVDQPRACVLSDWSVWSPCSVSCGVGVSESRRYVVTEPQNGGQPCSKRLVK 754          

HSP 3 Score: 71.633 bits (174), Expect = 1.063e-11
Identity = 38/109 (34.86%), Postives = 56/109 (51.38%), Query Frame = 0

Query:    7 SLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVE-ERFW 114
            + ++ L G +T    + F  F +  +             R P   +  R G FQL  D LT+F+D C NTV++   + K+E+QV+W+AP SGSGCV+    V E  R W
Sbjct:   60 TYNLLLLGSRTHLKVQHFTHFTITAEAHT--------GARRPQAASPRRVGRFQLFSDSLTQFNDRCVNTVSEADDLPKTEVQVMWVAPESGSGCVSLSAMVYEGPRAW 160          

HSP 4 Score: 53.1434 bits (126), Expect = 5.935e-6
Identity = 42/163 (25.77%), Postives = 63/163 (38.65%), Query Frame = 0

Query:  314 PGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDR--QLVQKEMCSSEVP-------------------KCEGSSGFYANAP-----------------GDFLPDD---------------------MCATSEWTKWSECSTTCGKGFRSRTRRFF 417
            P C  S+WSDWS CS TCG+G+++RTR  L+        C +  +L Q++ C + +                     C G+   YA  P                  +FL ++                      C  S+W+ WS CS +CG G  S +RR+ 
Sbjct:  576 PQCPTSQWSDWSPCSSTCGRGVTIRTRLLLLENGPDKESCTQRMELHQQKECVNPIDCHINAEQAKDICVQAPDPGPCRGTYMRYAYDPQNQHCYSFTYGGCRGNRNNFLTENDCLNTCNVLRSPYSSRVDQPRACVLSDWSVWSPCSVSCGVGV-SESRRYV 737          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:CG17739 species:7227 "Drosophila melanogaster" [GO:0005576 "extracellular region" evidence=ISS] [GO:0004867 "serine-type endopeptidase inhibitor activity" evidence=IEA] InterPro:IPR002223 Pfam:PF00014 PROSITE:PS50279 SMART:SM00131 EMBL:AE013599 GO:GO:0010951 GO:GO:0004867 Gene3D:4.10.410.10 SUPFAM:SSF57362 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 eggNOG:NOG279286 GeneTree:ENSGT00740000115000 OrthoDB:EOG71CFK9 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:AY058267 RefSeq:NP_610757.1 UniGene:Dm.4212 STRING:7227.FBpp0087006 EnsemblMetazoa:FBtr0087893 GeneID:36333 KEGG:dme:Dmel_CG17739 UCSC:CG17739-RA FlyBase:FBgn0033710 InParanoid:Q7K3Y9 OMA:CVVFRAT GenomeRNAi:36333 NextBio:797969 Uniprot:Q7K3Y9)

HSP 1 Score: 292.738 bits (748), Expect = 4.326e-86
Identity = 154/319 (48.28%), Postives = 189/319 (59.25%), Query Frame = 0

Query:  108 VVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKC--------EGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFN 418
             ++ RFW+YG  AS+GL +VA+ GS R LESELK +S+HIRTIIKARG+ YPN  GKTFAVFR D  +HL+SLVSM+ PSPDWIVGVSGLELC  NC+WVE K   LYPWDAG DSG SY S D P  P   + RI S FPNDPR PFYD  G  MKPLA L +NR+RLY+K+C   D                        ++  P C  + WS W  C+  CG G   R R+F  P  AS   C+  L +++ C     KC        EG     A +PG    D  C  S+W++WS C+ TCG G  +R+R + N
Sbjct:  223 TLDYRFWQYGELASEGLREVAEHGSTRTLESELKDQSEHIRTIIKARGIAYPNVTGKTFAVFRVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQPQVPPDVVRRIKSNFPNDPRSPFYDPTGAQMKPLATLHINRRRLYEKNCESSD-----------------------SEQVPPECATNSWSRWDECTTKCGPGKQYRIREFKNPALASRHRCNNALREEKNCVGH--KCAGFNEETAEGGEPEVAPSPGSS-DDPQCGLSDWSEWSSCTVTCGTGEMTRSRHYLN 515          

HSP 2 Score: 57.7658 bits (138), Expect = 2.219e-7
Identity = 44/137 (32.12%), Postives = 58/137 (42.34%), Query Frame = 0

Query:  309 EDESIPG--CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSG-------FYANAPGDFLPDDMCATSEWTKWSECSTTCG-KGFRSRTRRFFNRMGRKKCPHIDTIEKLM 435
            + + IP   C VS+W   S C+ +CG G  +RTR  L   K     C + LV+ + C          SG           AP    P D C  SEW+ W+ C+ TCG    R RTR   N     KC     +EK +
Sbjct:  724 QHDGIPAIDCLVSDWVSHS-CNASCGDGFQLRTRRVLRTPKYGGKPCPKHLVRLDRCYQRCDDMYSISGRGFGERRHVVRAPQP-EPQDECRYSEWSAWTPCTATCGDNAVRQRTRTLMNTDLSYKCKDRVRMEKCV 858          

HSP 3 Score: 51.2174 bits (121), Expect = 2.735e-5
Identity = 32/103 (31.07%), Postives = 51/103 (49.51%), Query Frame = 0

Query:   11 RLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPS-GSGCVNFKTTVVEER 112
            R +   + F    F  F+L +D +  DG            DA+  G ++++     TRFS  C N V  T++ VK+ + V W+AP S G GC+  + TV++ R
Sbjct:   57 RYNVSLSAFSGLSFISFMLALDLESGDGDG----------DANALG-TWEIADLAETRFSPRCPNLVENTNTNVKTRVDVFWVAPSSPGQGCILLRATVMQHR 148          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:spon-1 "Protein SPON-1" species:6239 "Caenorhabditis elegans" [GO:0004867 "serine-type endopeptidase inhibitor activity" evidence=IEA] [GO:0005604 "basement membrane" evidence=IDA] [GO:0007413 "axonal fasciculation" evidence=IMP] [GO:0008045 "motor neuron axon guidance" evidence=IMP] [GO:0010951 "negative regulation of endopeptidase activity" evidence=IEA] [GO:0016203 "muscle attachment" evidence=IMP] [GO:0031430 "M band" evidence=IDA] [GO:0033563 "dorsal/ventral axon guidance" evidence=IGI;IMP] [GO:0033627 "cell adhesion mediated by integrin" evidence=IGI] [GO:0055120 "striated muscle dense body" evidence=IDA] InterPro:IPR002223 Pfam:PF00014 PRINTS:PR00759 PROSITE:PS50279 SMART:SM00131 GO:GO:0010951 GO:GO:0031430 GO:GO:0004867 GO:GO:0055120 Gene3D:4.10.410.10 InterPro:IPR020901 SUPFAM:SSF57362 PROSITE:PS00280 GO:GO:0008045 GO:GO:0016203 GO:GO:0033627 GO:GO:0005604 GO:GO:0007413 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 GO:GO:0033563 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 EMBL:FO081111 eggNOG:NOG279286 GeneTree:ENSGT00740000115000 OrthoDB:EOG71CFK9 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 PIR:T34212 RefSeq:NP_495473.2 UniGene:Cel.34220 ProteinModelPortal:Q19305 SMR:Q19305 STRING:6239.F10E7.4 PaxDb:Q19305 PRIDE:Q19305 EnsemblMetazoa:F10E7.4 GeneID:174169 KEGG:cel:CELE_F10E7.4 UCSC:F10E7.4 CTD:174169 WormBase:F10E7.4 HOGENOM:HOG000230764 InParanoid:Q19305 OMA:IWSNETH NextBio:882825 PRO:PR:Q19305 Uniprot:Q19305)

HSP 1 Score: 270.781 bits (691), Expect = 2.325e-78
Identity = 161/533 (30.21%), Postives = 265/533 (49.72%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFY--------------------------------ANAPGD--------------------FLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTAD--CSLDNEECDRICKS---------------LNNSTSAGHSFII---DMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRL 573
             W  GG ++ G++++A+WG+    E+E KA++  +R+++K +GLW+P+ +G T + F  +K +H VSL +M GPSPDW VG+S + LC  +CTW EE+   L P+DAG DSG +Y SP+ PTEP+ PI  I+++   +P  PFY+     +  LAK+IL R+ +    C  +D L  +   H  +  S++EE     +     C +++W  WS CS TCGKG+ +R+R ++ P KA +  C RQ  +K+ C++++ +CE S  F                                 A   GD                      PD +C T+ W+ WS CS +C +G R RTR FF     K+C H++  EK  C   +      +++EE   IC+                 N+  +    FI       + Q   E++ K++ +  +E  K+ I        + ++  N + D+G  R DC V+ W+ W  C+ SCG+G + + R ++  + + G + S+   +E + RL
Sbjct:  239 LWTIGGISTDGMKEIAEWGNTYKAEAEAKAKASEVRSLMKVKGLWFPDVQGTTKSQFVVNKYHHFVSLATMFGPSPDWCVGLSSVNLCLPDCTWAEERTFELQPFDAGTDSGPTYMSPNEPTEPREPIHWITTKL--NPLSPFYNSKSDTIPTLAKVILRRKNVTSSECKSDDILKAE--AHNITNTSEDEEYKDRRE-----CMMTQWEPWSLCSATCGKGIRIRSRVYVFPIKAQVFHCHRQTTEKQFCNAKINECENSEAFSSKCQVSSWGSWGECSVQCGHGMRSRNRTFLNPATKSGDCSVDLERKDICVGENGDDCNVTPDPLCKTTAWSDWSPCSASCDEGVRVRTRLFFYSEHEKRCMHVNLQEKDTCVMQSCRRFIEINSEE---ICQEDKQAGQCAGNFPRYWYNHEKTQCERFIFTGCKGNRNQFETEEECKQICLPGYEKSKSLI----PNNQLLEDFGNTEVDDGGERVDCEVSKWTAWGSCSVSCGRGKKSRSRHVVKLARNGGHQCSEHLMQELQCRL 755          

HSP 2 Score: 50.8322 bits (120), Expect = 3.719e-5
Identity = 27/93 (29.03%), Postives = 42/93 (45.16%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKG 408
            C+VS+W+ W +CSV+CG+G   R+R  +   +     C   L+Q+  C                 P        C    W++WS CS +CG+G
Sbjct:  706 CEVSKWTAWGSCSVSCGRGKKSRSRHVVKLARNGGHQCSEHLMQELQCR------------LRPCPVKL----TCQVGPWSRWSPCSVSCGEG 782          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:CG30203 species:7227 "Drosophila melanogaster" [GO:0004867 "serine-type endopeptidase inhibitor activity" evidence=IEA] InterPro:IPR002223 Pfam:PF00014 PROSITE:PS50279 SMART:SM00131 EMBL:AE013599 GO:GO:0010951 GO:GO:0004867 Gene3D:4.10.410.10 SUPFAM:SSF57362 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 GeneTree:ENSGT00740000115000 OrthoDB:EOG71CFK9 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:BT023853 RefSeq:NP_725128.2 UniGene:Dm.23753 EnsemblMetazoa:FBtr0273303 GeneID:246514 KEGG:dme:Dmel_CG30203 UCSC:CG30203-RB FlyBase:FBgn0050203 eggNOG:NOG244582 OMA:HECKIFT GenomeRNAi:246514 NextBio:842774 Uniprot:Q3ZAL6)

HSP 1 Score: 243.047 bits (619), Expect = 5.298e-68
Identity = 140/368 (38.04%), Postives = 191/368 (51.90%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESELK--AESDHIRTIIKARGLWY-PNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSS-------------------EVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTI---EKLMCAGNTADCS---LDNEECD 451
            R+W YGG ASKG++++A+ G+ R LE+E++   ++  +RTIIKA G+ Y P   G T A  R D  +H +SL + + PSPDWI+GV+GLELC  NCTW+E K L LYPWD G DSG SY S D P  P   + RI+S +P+D R PFYDD G  MKPLA L L R++LY + C +   +P++  +                      C V  W++W+ CS  CG+G S R R F  P  A+   CDR+L +   C                     E P   GS                C  + W +WS C+ TCG+G  +RTR ++N   R++C  +  I   E   C G  +DC     DN E D
Sbjct:  224 RYWAYGGRASKGMKELAEHGATRTLENEIRENTQNGEVRTIIKAPGIPYRPKTFGTTLANARLDPVHHQISLAAKIDPSPDWILGVAGLELCLSNCTWLERKVLNLYPWDIGTDSGPSYMSSDEPQVPPDVVRRITSSYPSDHRSPFYDDTGAPMKPLATLYLTRKKLYIRECEE---VPQEGPLE---------------------CAVHPWNEWTNCSTRCGQGYSHRQRSFKNPNLAANFNCDRRLEEFRQCQGTQCGAAEEELEGEEPGLGMENPPSSGSMA-------------ECQLTNWGEWSPCTKTCGRGSSTRTRDYYNPQARQRCLSVMRIPLEETRECIG--SDCGGTIPDNGELD 552          

HSP 2 Score: 51.2174 bits (121), Expect = 2.850e-5
Identity = 38/94 (40.43%), Postives = 48/94 (51.06%), Query Frame = 0

Query:   20 IERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPS-GSGCVNFKTTVVEER 112
            I  KF  F LVV  + +D S+ F        D +G    F+LLG   TRFS  C N V  T++  K  I V W+AP +  SGCV  K  VV+ R
Sbjct:   62 INMKFVSFTLVV--EAEDPSAAFGGQ-----DMTGH---FELLGVGDTRFSTSCENMVENTNTNAKIYIAVSWIAPRNPDSGCVLIKAGVVQHR 145          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:CG30046 species:7227 "Drosophila melanogaster" [GO:0004867 "serine-type endopeptidase inhibitor activity" evidence=IEA] InterPro:IPR002223 EMBL:AE013599 GO:GO:0010951 GO:GO:0004867 SUPFAM:SSF57362 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 GeneTree:ENSGT00740000115000 OrthoDB:EOG71CFK9 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:BT133491 RefSeq:NP_725129.1 UniGene:Dm.14461 STRING:7227.FBpp0087008 EnsemblMetazoa:FBtr0087895 GeneID:36334 KEGG:dme:Dmel_CG30046 UCSC:CG30046-RB FlyBase:FBgn0050046 eggNOG:NOG277984 InParanoid:A1Z8W6 OMA:CACDEAR GenomeRNAi:36334 NextBio:797974 Uniprot:A1Z8W6)

HSP 1 Score: 236.113 bits (601), Expect = 6.374e-66
Identity = 136/339 (40.12%), Postives = 172/339 (50.74%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESE--LKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIP-GCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAP--GDFLPDDM-------------CATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIE 432
            RFWE G  AS  +++ A+  S R+LE E  +      IRTIIKARG  YPN   KT A  R D  +H+VS  S + PSPDWIVGV+GLELC +NCTW+EEK + LYPWD G DSG +Y S D P  P   I R+ S FPNDPR PFYD +G  MKP+A L + RQR+Y++ C+DED                        D  +P  C    WS WS CS  CG G+  R R +  P  A +  C+    ++  C  E+  C G +          D  PD               C  S W  WS CS TCG G+  R R++ N      C  +  +E
Sbjct:  235 RFWESGELASVAMKEYAEHCSSRLLEREFSINFRDQKIRTIIKARGPSYPNISSKTMASVRVDPIHHMVSFASKIEPSPDWIVGVTGLELCLRNCTWMEEKVINLYPWDVGTDSGPTYTSSDQPQVPPDVIRRMRSDFPNDPRSPFYDISGTPMKPMAILTVKRQRIYERRCADED---------------------SNNDPDVPRECFTHPWSSWSDCSSKCGVGMQYRRRVYKQPELARVYNCNEAQYEERECQGEM--C-GQNNLMREPEEFEDLEPDPRRIGYQPSQQRRAECELSSWGSWSPCSVTCGDGYEMRQRQYLNPQAEFDCQGVHRME 549          

HSP 2 Score: 48.1358 bits (113), Expect = 2.387e-4
Identity = 38/125 (30.40%), Postives = 56/125 (44.80%), Query Frame = 0

Query:   57 GSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPS-GSGCVNFKTTVVEER-FWEY--GGYASKGLEQVAKWGSPRILESELKAES---------DHIRTIIKARGLWYPNHEGKTFAV 168
            G F+L   + TRFS +C N V  T++  K+ + + W+AP   GSGCV  + TV + R  W    GG   +  E+V        +ES+  A +         D  R  +   G+W  N   K F  
Sbjct:   96 GRFELSDLIETRFSPNCINMVENTNTNSKTHMHLTWVAPSEPGSGCVLIRATVQQHREVWHMDDGGLTKRICEEVTD-----DVESQPTAPAAVDVPCCACDEARYELIFEGVWSRNLHPKDFPT 215          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:SPON1 "Spondin-1" species:9031 "Gallus gallus" [GO:0005578 "proteinaceous extracellular matrix" evidence=IEA] [GO:0007155 "cell adhesion" evidence=IEA] GO:GO:0005578 GO:GO:0007155 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 CTD:10418 eggNOG:NOG279286 HOGENOM:HOG000124667 HOVERGEN:HBG052920 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:AF149302 RefSeq:NP_990182.1 UniGene:Gga.3330 ProteinModelPortal:Q9W770 SMR:Q9W770 STRING:9031.ENSGALP00000039229 PaxDb:Q9W770 GeneID:395657 KEGG:gga:395657 InParanoid:Q9W770 NextBio:20815729 PRO:PR:Q9W770 Uniprot:Q9W770)

HSP 1 Score: 213.001 bits (541), Expect = 6.366e-58
Identity = 150/446 (33.63%), Postives = 218/446 (48.88%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++M+GPSPDW VG+S  +LC K+C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +K +A+++L R     + C   +F+P D      + ++ EE+   EED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  S+W  WS CS +CG G RSR R        K+ P   ++ K+     T  C + NEEC     +            S    MK++  M++    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  240 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTYESPNKPTVPQEKIRPLTSL--DHPQSPFYDPEGGSIKLVARVVLERIARKGEQC---NFVP-DNIDDIVADLAPEEK---EEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCMMSDWITWSPCSVSCGMGTRSRERYV------KQFPEDGSMCKVP-TEETEKC-IVNEECSPSSCLVTEWGEWDECSASCGTGMKRRHRMIKMTPADGSMCKAETTEAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 636          

HSP 2 Score: 83.1889 bits (204), Expect = 2.496e-15
Identity = 72/271 (26.57%), Postives = 118/271 (43.54%), Query Frame = 0

Query:  288 PEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTR--RFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSS 556
            P D SM  ++  ++ E+  + E  +IP C +S WS+WS CSVTCGKG  MRTR  ++   A +  C+ +L Q E C   +P+C               P D C  +EW++WSEC+T+CGKG   RTR  +   + G   CP  +T+++  C                                             R    ++   M K       +K+  ++  KN+ N++      C +  W+ W++C+  CG G + ++  +  +S S
Sbjct:  584 PADGSM-CKAETTEAEKCMMPECHTIP-CLLSPWSEWSDCSVTCGKG--MRTRQRMLKSAAELGDCNEELEQAEKCM--LPEC---------------PID-CELTEWSQWSECNTSCGKGHMIRTRMIKIEPQFGGTACP--ETVQRTKC---------------------------------------------RVRKCLRGPGMEKRRWKEAREKRRSEQAKKNIDNEQ---YPVCRLKPWTAWTECSTLCGGGIQERYMMVKKRSKS 782          

HSP 3 Score: 58.151 bits (139), Expect = 1.781e-7
Identity = 32/106 (30.19%), Postives = 54/106 (50.94%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W APP+G+GCV  K ++V++R   F + G    +  EQ
Sbjct:   85 FRGFTLIALKEGKEGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICEQ 179          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:Spon1 "spondin 1, (f-spondin) extracellular matrix protein" species:10090 "Mus musculus" [GO:0005576 "extracellular region" evidence=IEA] [GO:0005578 "proteinaceous extracellular matrix" evidence=IEA] [GO:0005615 "extracellular space" evidence=ISO] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0031012 "extracellular matrix" evidence=ISO] MGI:MGI:2385287 GO:GO:0005578 GO:GO:0007155 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 CTD:10418 eggNOG:NOG279286 GeneTree:ENSGT00740000115000 HOGENOM:HOG000124667 HOVERGEN:HBG052920 OMA:IRQQSDE OrthoDB:EOG71CFK9 TreeFam:TF313353 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 ChiTaRS:SPON1 EMBL:BC020531 EMBL:BC030339 RefSeq:NP_663559.1 UniGene:Mm.334160 ProteinModelPortal:Q8VCC9 SMR:Q8VCC9 IntAct:Q8VCC9 MINT:MINT-4135381 PhosphoSite:Q8VCC9 PaxDb:Q8VCC9 PRIDE:Q8VCC9 Ensembl:ENSMUST00000046687 GeneID:233744 KEGG:mmu:233744 UCSC:uc009jhv.2 InParanoid:Q8VCC9 NextBio:381807 PRO:PR:Q8VCC9 ArrayExpress:Q8VCC9 Bgee:Q8VCC9 Genevestigator:Q8VCC9 Uniprot:Q8VCC9)

HSP 1 Score: 212.231 bits (539), Expect = 1.020e-57
Identity = 148/446 (33.18%), Postives = 217/446 (48.65%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  ++    T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSVSCGMGMRSRERYV------KQFPEDGSV-CMLPTEETEKCTV-NEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 82.8037 bits (203), Expect = 4.293e-15
Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C V+EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++  EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K    K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSVCMLP-TEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSC---------GKGHMIRTRTIQMEPQFGGVPCPETVQRKKCRTRKCLRSPSVQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.4622 bits (145), Expect = 3.086e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENQEGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:Spon1 "Spondin-1" species:10116 "Rattus norvegicus" [GO:0005578 "proteinaceous extracellular matrix" evidence=IEA] [GO:0007155 "cell adhesion" evidence=IEA] RGD:619918 GO:GO:0005578 GO:GO:0007155 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 CTD:10418 eggNOG:NOG279286 HOGENOM:HOG000124667 HOVERGEN:HBG052920 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:M88469 PIR:A38152 RefSeq:NP_742064.1 UniGene:Rn.7546 PDB:1SZL PDB:1VEX PDBsum:1SZL PDBsum:1VEX ProteinModelPortal:P35446 SMR:P35446 IntAct:P35446 STRING:10116.ENSRNOP00000046944 PaxDb:P35446 PRIDE:P35446 GeneID:64456 KEGG:rno:64456 UCSC:RGD:619918 EvolutionaryTrace:P35446 NextBio:613188 PRO:PR:P35446 Genevestigator:P35446 Uniprot:P35446)

HSP 1 Score: 212.231 bits (539), Expect = 1.175e-57
Identity = 148/446 (33.18%), Postives = 217/446 (48.65%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  ++    T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPSWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSVSCGMGMRSRERYV------KQFPEDGSV-CMLPTEETEKCTV-NEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 82.4185 bits (202), Expect = 5.062e-15
Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C V+EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++  EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K    K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSVCMLP-TEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSC---------GKGHMIRTRTIQMEPQFGGAPCPETVQRKKCRARKCLRSPSIQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 4.280e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:Spon1 "spondin 1, extracellular matrix protein" species:10116 "Rattus norvegicus" [GO:0005578 "proteinaceous extracellular matrix" evidence=IEA] [GO:0005615 "extracellular space" evidence=ISO] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0031012 "extracellular matrix" evidence=ISO] RGD:619918 GO:GO:0005578 GO:GO:0007155 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 CTD:10418 eggNOG:NOG279286 HOGENOM:HOG000124667 HOVERGEN:HBG052920 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:M88469 PIR:A38152 RefSeq:NP_742064.1 UniGene:Rn.7546 PDB:1SZL PDB:1VEX PDBsum:1SZL PDBsum:1VEX ProteinModelPortal:P35446 SMR:P35446 IntAct:P35446 STRING:10116.ENSRNOP00000046944 PaxDb:P35446 PRIDE:P35446 GeneID:64456 KEGG:rno:64456 UCSC:RGD:619918 EvolutionaryTrace:P35446 NextBio:613188 PRO:PR:P35446 Genevestigator:P35446 Uniprot:P35446)

HSP 1 Score: 212.231 bits (539), Expect = 1.175e-57
Identity = 148/446 (33.18%), Postives = 217/446 (48.65%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  ++    T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPSWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSVSCGMGMRSRERYV------KQFPEDGSV-CMLPTEETEKCTV-NEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 82.4185 bits (202), Expect = 5.062e-15
Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C V+EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++  EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K    K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSVCMLP-TEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSC---------GKGHMIRTRTIQMEPQFGGAPCPETVQRKKCRARKCLRSPSIQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 4.280e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. GO
Match: - (symbol:SPON1 "Spondin-1" species:9606 "Homo sapiens" [GO:0005515 "protein binding" evidence=IPI] [GO:0005578 "proteinaceous extracellular matrix" evidence=IEA] [GO:0005615 "extracellular space" evidence=IDA] [GO:0007155 "cell adhesion" evidence=IEA] [GO:0031012 "extracellular matrix" evidence=ISS;IDA] GO:GO:0005615 EMBL:CH471064 GO:GO:0005578 GO:GO:0007155 InterPro:IPR000884 Pfam:PF00090 SMART:SM00209 SUPFAM:SSF82895 PROSITE:PS50092 InterPro:IPR002861 Pfam:PF02014 PROSITE:PS51019 CTD:10418 eggNOG:NOG279286 HOGENOM:HOG000124667 HOVERGEN:HBG052920 OMA:IRQQSDE OrthoDB:EOG71CFK9 InterPro:IPR009465 Pfam:PF06468 PROSITE:PS51020 EMBL:AB051390 EMBL:AK074803 EMBL:AK291780 EMBL:BC019825 EMBL:BC136513 EMBL:BC136563 EMBL:AB018305 RefSeq:NP_006099.2 UniGene:Hs.643864 PDB:2ZOT PDB:2ZOU PDB:3COO PDB:3Q13 PDBsum:2ZOT PDBsum:2ZOU PDBsum:3COO PDBsum:3Q13 ProteinModelPortal:Q9HCB6 SMR:Q9HCB6 IntAct:Q9HCB6 STRING:9606.ENSP00000309297 PhosphoSite:Q9HCB6 DMDM:52783472 PaxDb:Q9HCB6 PRIDE:Q9HCB6 Ensembl:ENST00000576479 GeneID:10418 KEGG:hsa:10418 UCSC:uc001mle.3 GeneCards:GC11P013940 H-InvDB:HIX0009464 HGNC:HGNC:11252 MIM:604989 neXtProt:NX_Q9HCB6 PharmGKB:PA36082 InParanoid:Q9HCB6 ChiTaRS:SPON1 EvolutionaryTrace:Q9HCB6 GeneWiki:Spondin_1 GenomeRNAi:10418 NextBio:39482 PRO:PR:Q9HCB6 CleanEx:HS_SPON1 Genevestigator:Q9HCB6 Uniprot:Q9HCB6)

HSP 1 Score: 211.846 bits (538), Expect = 1.517e-57
Identity = 148/446 (33.18%), Postives = 216/446 (48.43%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  L     T  C++ NEEC     +            +  + MKK+  M++    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSISCGMGMRSRERYV------KQFPEDGSVCTLP-TEETEKCTV-NEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 84.7297 bits (208), Expect = 1.070e-15
Identity = 73/280 (26.07%), Postives = 118/280 (42.14%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C ++EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++ +EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K  + K       + +   + ++ ++  +  +  EG     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSV-CTLPTEETEKCTVNEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNKSC---------GKGHVIRTRMIQMEPQFGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRSEQLKEESEGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 4.954e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALRENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592805388|gb|GAXK01149180.1| (TSA: Calanus finmarchicus comp96244_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 443.351 bits (1139), Expect = 1.043e-142
Identity = 209/345 (60.58%), Postives = 256/345 (74.20%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIP-GCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR 452
            +  R WE GG ASKGL QVAKWGSPR+LESELKAES HIRTIIKARGLW+PN  GKTFA+FRTD KNHLVSLVSM+GPSPDWIVGVS LE+C+ NCTW++ K++FLYPWDAGVDSGISYES D PT P  PI RI+S+ P D   PF+   G  +KP+AKL + + R YKKSCSD+    + + +H          Q  +    +P GC+ S+WS+WS CSVTCGKG+SMR R ++ P  A  +GCDRQ+V+KEMC+SEV  C+G + FY++AP D++PDD CAT+EWT WS CS  CG GFR+RTRRFFNRMGRKKCPH++T+ K  C G    C+ +  E  R
Sbjct: 1120 INYRVWEAGGRASKGLTQVAKWGSPRMLESELKAESKHIRTIIKARGLWHPNVNGKTFAIFRTDSKNHLVSLVSMLGPSPDWIVGVSALEMCQANCTWLDRKDIFLYPWDAGVDSGISYESQDQPTSPNQPIKRITSQNPPDENNPFFKSEGGPLKPIAKLSIQKLREYKKSCSDDLTESKHQFVH----------QTFDSSSLVPAGCETSDWSEWSGCSVTCGKGISMRKRKYVDPFTADNIGCDRQMVEKEMCASEVAVCQGETAFYSSAPSDWVPDDTCATTEWTDWSACSVPCGTGFRARTRRFFNRMGRKKCPHVETVMKQACQGERDICAPEEVEIVR 2124          

HSP 2 Score: 105.145 bits (261), Expect = 1.289e-22
Identity = 52/102 (50.98%), Postives = 69/102 (67.65%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEE 111
            +RL GDK +F +++F+ F+L         S   D HR   +  SG  G+FQL GD+ T+FSDHCTNT+T+TS V K EIQVLW+AP +G GCV FK TV+E+
Sbjct: 2350 LRLRGDKDKFYQKQFQSFVLT--------SVNGDPHR---VAESGSAGTFQLFGDIHTKFSDHCTNTITETSGVAKDEIQVLWVAPSAGRGCVQFKATVLEQ 2622          

HSP 3 Score: 91.6633 bits (226), Expect = 2.713e-18
Identity = 93/322 (28.88%), Postives = 139/322 (43.17%), Query Frame = 0

Query:  314 PGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASM-MGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMC--ATSEW---TKWSECSTTCGKGFRSRTRRFFNRMG-RKKCPHIDTIEKLMCAGNTADCSLDN---EECDRICKSLNNSTSAG--HSFIIDMKKKQMMV----EQQRKELV--------------------------MKQHEMMKTEIMMLNKKKDM----QKEMKNMQNDEG---------------ISRSDCSVTSWSPWS-QCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRL 573
            P C V++WS+WS CS +CG GL +RTR + +  +  +  GC  QL+QK  C  E   CE   G   +   +  P   C  A   W   TK   C      G R  +  F       K+C      ++    G   D  L++   +  D + K   +  S G    FI   +++Q++     EQ+  E                             KQ  M K + MM+ K+++M    QKEM +   D+G               I R DCSVT+WSPWS QC++SCG+G+R +FRT+   +   G+   +K  ++ K RL
Sbjct:  181 PECGVTDWSNWSPCSQSCGSGLKVRTRLYRVSKEQQVEEGCSVQLMQKGTCKGEDKMCEADGGTVCSQEREIGP---CRGAFKRWYFDTKTGVCQEFFFGGCRGNSNNFMKYEDCEKRCS-----KQSFGTGGMEDMYLNDGFKDALDVLVKKRRDDRSEGMNEGFIEIEEQRQVLQGLENEQRNAEATGEKFLKMGELMAAQKKLMMMEKRMMMDKQMAMFKQKQMMMAKQRNMVEQKQKEMMSGLFDKGQDTLTNTSVAATIGDIDR-DCSVTAWSPWSVQCSSSCGRGYRHRFRTVQQDAQGDGRPCPRKLDRQKKCRL 1119          

HSP 4 Score: 49.2914 bits (116), Expect = 3.692e-5
Identity = 38/123 (30.89%), Postives = 57/123 (46.34%), Query Frame = 0

Query:  316 CQVSEWSDWST-CSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGK-GFRSRTRRFFNRMGRKKCPHIDTIEKLMC 436
            C V+ WS WS  CS +CG+G   R R      +     C R+L +++ C   +P C               PD+ C  + W  W  CS TCG  G ++R R   + +G  +C   DT+E+ +C
Sbjct:   34 CSVTAWSPWSVQCSSSCGRGYRHRFRTVQQDAQGDGRPCPRKLDRQKKC--RLPPC---------------PDN-CQLTSWEDWGPCSHTCGSDGTQTRHR---SSVGLTQCG--DTVEERVC 333          

HSP 5 Score: 48.521 bits (114), Expect = 5.963e-5
Identity = 38/162 (23.46%), Postives = 57/162 (35.19%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTIL 551
            C TS+W++WS CS TCGKG   R R++ +                         + DN  CDR                        MVE+          EM  +E+ +   +        +    + +    C+ T W+ WS C+  CG GFR + R   
Sbjct: 1225 CETSDWSEWSGCSVTCGKGISMRKRKYVDPF-----------------------TADNIGCDR-----------------------QMVEK----------EMCASEVAVCQGETAFYSSAPS----DWVPDDTCATTEWTDWSACSVPCGTGFRARTRRFF 1530          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592893017|gb|GAXK01065358.1| (TSA: Calanus finmarchicus comp5588_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 187.578 bits (475), Expect = 7.683e-49
Identity = 148/509 (29.08%), Postives = 231/509 (45.38%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWY-PNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDES-------------MHFESGVSK-----------------EEEQGVEEDESIPG------------CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTA---DCSLDNEECDRICKSLN--NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTIL-VQSSSHGKKMSKKTGKE 568
            ++   W+YG  +S  L  +A+ G  + LE ++K  S +IR++IKARGL    N  G+TFAVFR D   HL+SLVS + PSPDWIVGVS   LC  N +WV+ + + LYPWDAG +SG++Y+     T+P+  I RI+S  PND R PF+D     +KP+A+L + +QR YKK C   D LP + S             ++  +G+                   +  Q   +D +  G            C VS+WS W+ CS +C  G   R+R F     A +  C     +K+ C + +PKCE  S  YA   G+F  D   +  E ++++    T G   +S T+   +     K    D          ++   +  +D +  D      N  N  +    +               K++    +          N+   +   ++    ++  +   C VT W  WS C+ +CG G R + R    ++SSS  K++ + T  E
Sbjct: 1025 IDYDLWKYGELSSPALTMLAETGMTKKLEIDMKRFSKNIRSVIKARGLQQRSNVVGRTFAVFRMDATKHLLSLVSKIIPSPDWIVGVSMENLCLANGSWVDSRIVDLYPWDAGTNSGLTYQERGEDTQPRASIHRITSCTPNDERSPFFDQTCAPVKPVARLHILKQREYKKECEGGDPLPLNPSWGSPNAPSINGDNVYAGTGIGPNLQSESATEPQYSYGDYDNSQTYRDDGNSYGNTYGGYGRNSNPCDVSDWSSWTQCSQSCDSGSQSRSRQFHNVIGAQIGNCKTTTFEKQPCRN-LPKCETKS--YA---GNF--DPFFSQDELSEFTSPWNTKGLVSKSLTKEEESPNANSKSYLYDGNSSPQVGSPSSYNYNSGMDTQ-IDPYSDPYNKYNGYAKQKGYPPKHNHPSSNRYNPYKDMGYYGYTYGGPPTGRFNQPSYVSDSLEAEDREQSDATPLCEVTEWGDWSHCSTNCGTGTRTRTRHYTEMESSSCSKELFEDTSCE 2524          

HSP 2 Score: 72.4034 bits (176), Expect = 3.063e-12
Identity = 53/170 (31.18%), Postives = 81/170 (47.65%), Query Frame = 0

Query:    3 IYEFSLH-IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER-FW--EYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAV 168
            +Y  SL  +R+D    +  + KF +F++V +               PS + SG G    + GD +++FS  C++ V  TS++ K E+ VLW AP  GSGC+N K  VVE   FW  + G       E  +   +P ++E   KA  D  +  +   GLW  +   K F V
Sbjct:  509 VYTVSLAGMRMDEGDIEPKKVKFIDFMIVAESDL------------PSTEVSGLGTFQTMPGDAMSKFSHKCSHAVMATSALSKEEVHVLWTAPEMGSGCINLKAMVVERNDFWFMDDGALTYTICEDDSPMAAPPLVEP-CKA-CDEAKYEVIFEGLWSRHTHPKDFPV 976          

HSP 3 Score: 69.707 bits (169), Expect = 1.973e-11
Identity = 69/261 (26.44%), Postives = 113/261 (43.30%), Query Frame = 0

Query:  299 VSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGK 559
            VS++ +Q    D   P C+V++W++WS CSVTC  G  +RTR +++P   + +  + +L QK  C  E   C  S  +  N        D    +E    +E S T   G    T +   +  +  C   +      C GN+     +  E    C +   +   G+    + K +   +E     +  K+   MK+  ++   ++D   E        G   +DC V+ WS WS C+ASCG+G+    RTIL +    GK
Sbjct: 2636 VSRKRKQFTHGD---PQCEVAQWTEWSPCSVTCDNGYKIRTRIYMMPFVPNRICDNIRLTQKMDCRHE--SCWNSDYYDQN--------DSPPMNE----NEYSLTDDNG--PITIQIVEKPNQPYC--NEEHHPGYCIGNSEQWYFNATEGK--CATFKYTGCGGNKN--NFKTEAECLEVCHPNMPDKRFNSMKSLSLV---REDYMSEQPQQPVQTGPRHNDCQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGK 3334          

HSP 4 Score: 63.929 bits (154), Expect = 1.207e-9
Identity = 42/164 (25.61%), Postives = 70/164 (42.68%), Query Frame = 0

Query:  303 EEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASM-------------MGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLP----------------DDMCATSEWTKWSECSTTCGKGFRSRTRRFFNR-MGRKKCPHIDTIEKLMC 436
            E +  E+ ++ P C+V+EW DWS CS  CG G   RTR +     +S               GC   ++++ + ++  P+             + LP                D  C  ++WT+WS CS TC  G++ RTR +    +  + C +I   +K+ C
Sbjct: 2348 EAEDREQSDATPLCEVTEWGDWSHCSTNCGTGTRTRTRHYTEMESSSCSKELFEDTSCEESSGCSNDIIEETISTTMRPR------MRRPVVKNRLPTPRKQVSRKRKQFTHGDPQCEVAQWTEWSPCSVTCDNGYKIRTRIYMMPFVPNRICDNIRLTQKMDC 2821          

HSP 5 Score: 50.447 bits (119), Expect = 1.526e-5
Identity = 38/164 (23.17%), Postives = 65/164 (39.63%), Query Frame = 0

Query:  389 MCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            +C  +EW  WS CST CG G R+RTR +   M    C   +  E   C   ++ CS D                     II+      M  + R+ +V  +         +   +K + ++ K   + +      C V  W+ WS C+ +C  G++ + R  ++
Sbjct: 2384 LCEVTEWGDWSHCSTNCGTGTRTRTRHY-TEMESSSCSK-ELFEDTSCE-ESSGCSND---------------------IIEETISTTMRPRMRRPVVKNR---------LPTPRKQVSRKRKQFTHGD----PQCEVAQWTEWSPCSVTCDNGYKIRTRIYMM 2764          

HSP 6 Score: 43.1282 bits (100), Expect = 2.865e-3
Identity = 23/62 (37.10%), Postives = 32/62 (51.61%), Query Frame = 0

Query:  292 SMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGC 353
            S+  E  +S++ +Q V+       CQVSEWS+WS CS +CG+G     R  L   K     C
Sbjct: 3155 SLVREDYMSEQPQQPVQTGPRHNDCQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGKAC 3340          

HSP 7 Score: 39.2762 bits (90), Expect = 4.448e-2
Identity = 17/39 (43.59%), Postives = 23/39 (58.97%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRFFN--RMGRKKCP 426
            C  SEW++WS CS +CG+G+ S  R      + G K CP
Sbjct: 3227 CQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGKACP 3343          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592893018|gb|GAXK01065357.1| (TSA: Calanus finmarchicus comp5588_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 187.578 bits (475), Expect = 7.714e-49
Identity = 148/509 (29.08%), Postives = 231/509 (45.38%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWY-PNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDES-------------MHFESGVSK-----------------EEEQGVEEDESIPG------------CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTA---DCSLDNEECDRICKSLN--NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTIL-VQSSSHGKKMSKKTGKE 568
            ++   W+YG  +S  L  +A+ G  + LE ++K  S +IR++IKARGL    N  G+TFAVFR D   HL+SLVS + PSPDWIVGVS   LC  N +WV+ + + LYPWDAG +SG++Y+     T+P+  I RI+S  PND R PF+D     +KP+A+L + +QR YKK C   D LP + S             ++  +G+                   +  Q   +D +  G            C VS+WS W+ CS +C  G   R+R F     A +  C     +K+ C + +PKCE  S  YA   G+F  D   +  E ++++    T G   +S T+   +     K    D          ++   +  +D +  D      N  N  +    +               K++    +          N+   +   ++    ++  +   C VT W  WS C+ +CG G R + R    ++SSS  K++ + T  E
Sbjct: 1025 IDYDLWKYGELSSPALTMLAETGMTKKLEIDMKRFSKNIRSVIKARGLQQRSNVVGRTFAVFRMDATKHLLSLVSKIIPSPDWIVGVSMENLCLANGSWVDSRIVDLYPWDAGTNSGLTYQERGEDTQPRASIHRITSCTPNDERSPFFDQTCAPVKPVARLHILKQREYKKECEGGDPLPLNPSWGSPNAPSINGDNVYAGTGIGPNLQSESATEPQYSYGDYDNSQTYRDDGNSYGNTYGGYGRNSNPCDVSDWSSWTQCSQSCDSGSQSRSRQFHNVIGAQIGNCKTTTFEKQPCRN-LPKCETKS--YA---GNF--DPFFSQDELSEFTSPWNTKGLVSKSLTKEEESPNANSKSYLYDGNSSPQVGSPSSYNYNSGMDTQ-IDPYSDPYNKYNGYAKQKGYPPKHNHPSSNRYNPYKDMGYYGYTYGGPPTGRFNQPSYVSDSLEAEDREQSDATPLCEVTEWGDWSHCSTNCGTGTRTRTRHYTEMESSSCSKELFEDTSCE 2524          

HSP 2 Score: 72.4034 bits (176), Expect = 3.066e-12
Identity = 53/170 (31.18%), Postives = 81/170 (47.65%), Query Frame = 0

Query:    3 IYEFSLH-IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER-FW--EYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAV 168
            +Y  SL  +R+D    +  + KF +F++V +               PS + SG G    + GD +++FS  C++ V  TS++ K E+ VLW AP  GSGC+N K  VVE   FW  + G       E  +   +P ++E   KA  D  +  +   GLW  +   K F V
Sbjct:  509 VYTVSLAGMRMDEGDIEPKKVKFIDFMIVAESDL------------PSTEVSGLGTFQTMPGDAMSKFSHKCSHAVMATSALSKEEVHVLWTAPEMGSGCINLKAMVVERNDFWFMDDGALTYTICEDDSPMAAPPLVEP-CKA-CDEAKYEVIFEGLWSRHTHPKDFPV 976          

HSP 3 Score: 69.707 bits (169), Expect = 1.974e-11
Identity = 69/261 (26.44%), Postives = 113/261 (43.30%), Query Frame = 0

Query:  299 VSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGK 559
            VS++ +Q    D   P C+V++W++WS CSVTC  G  +RTR +++P   + +  + +L QK  C  E   C  S  +  N        D    +E    +E S T   G    T +   +  +  C   +      C GN+     +  E    C +   +   G+    + K +   +E     +  K+   MK+  ++   ++D   E        G   +DC V+ WS WS C+ASCG+G+    RTIL +    GK
Sbjct: 2636 VSRKRKQFTHGD---PQCEVAQWTEWSPCSVTCDNGYKIRTRIYMMPFVPNRICDNIRLTQKMDCRHE--SCWNSDYYDQN--------DSPPMNE----NEYSLTDDNG--PITIQIVEKPNQPYC--NEEHHPGYCIGNSEQWYFNATEGK--CATFKYTGCGGNKN--NFKTEAECLEVCHPNMPDKRFNSMKSLSLV---REDYMSEQPQQPVQTGPRHNDCQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGK 3334          

HSP 4 Score: 63.929 bits (154), Expect = 1.208e-9
Identity = 42/164 (25.61%), Postives = 70/164 (42.68%), Query Frame = 0

Query:  303 EEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASM-------------MGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLP----------------DDMCATSEWTKWSECSTTCGKGFRSRTRRFFNR-MGRKKCPHIDTIEKLMC 436
            E +  E+ ++ P C+V+EW DWS CS  CG G   RTR +     +S               GC   ++++ + ++  P+             + LP                D  C  ++WT+WS CS TC  G++ RTR +    +  + C +I   +K+ C
Sbjct: 2348 EAEDREQSDATPLCEVTEWGDWSHCSTNCGTGTRTRTRHYTEMESSSCSKELFEDTSCEESSGCSNDIIEETISTTMRPR------MRRPVVKNRLPTPRKQVSRKRKQFTHGDPQCEVAQWTEWSPCSVTCDNGYKIRTRIYMMPFVPNRICDNIRLTQKMDC 2821          

HSP 5 Score: 50.447 bits (119), Expect = 1.526e-5
Identity = 38/164 (23.17%), Postives = 65/164 (39.63%), Query Frame = 0

Query:  389 MCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            +C  +EW  WS CST CG G R+RTR +   M    C   +  E   C   ++ CS D                     II+      M  + R+ +V  +         +   +K + ++ K   + +      C V  W+ WS C+ +C  G++ + R  ++
Sbjct: 2384 LCEVTEWGDWSHCSTNCGTGTRTRTRHY-TEMESSSCSK-ELFEDTSCE-ESSGCSND---------------------IIEETISTTMRPRMRRPVVKNR---------LPTPRKQVSRKRKQFTHGD----PQCEVAQWTEWSPCSVTCDNGYKIRTRIYMM 2764          

HSP 6 Score: 43.1282 bits (100), Expect = 2.866e-3
Identity = 23/62 (37.10%), Postives = 32/62 (51.61%), Query Frame = 0

Query:  292 SMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGC 353
            S+  E  +S++ +Q V+       CQVSEWS+WS CS +CG+G     R  L   K     C
Sbjct: 3155 SLVREDYMSEQPQQPVQTGPRHNDCQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGKAC 3340          

HSP 7 Score: 39.2762 bits (90), Expect = 4.449e-2
Identity = 17/39 (43.59%), Postives = 23/39 (58.97%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRFFN--RMGRKKCP 426
            C  SEW++WS CS +CG+G+ S  R      + G K CP
Sbjct: 3227 CQVSEWSEWSPCSASCGRGWVSMQRTILAEPKFGGKACP 3343          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592847462|gb|GAXK01110082.1| (TSA: Calanus finmarchicus comp562080_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 82.0333 bits (201), Expect = 9.689e-16
Identity = 69/295 (23.39%), Postives = 123/295 (41.69%), Query Frame = 0

Query:  163 GKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDA------------------------GVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFF--NRMGRKKCPHIDTI 431
            G++      D K+ ++S +S + PSPDW +G+ GL+LC KN TW    E+ +   D G D+G ++ SP+ PT P  P+ +I++  P+ P   F+  +                         +  KPL +      R + +  +D++ +P + +M   + + + E+Q     +S+ G  +  + D S+      + L    +D          G    + + +    E  K   S                C    W+ WS CS TCG G   R R+    +R G ++CP +  +
Sbjct:   73 GQSKTTLMVDGKHSMISFISKIIPSPDWFIGLDGLDLC-KNGTWSSLVEMEVDLLDGGTDNGFTFTSPNYPTLPPSPVQKITNILPDHPAGSFHYPSLASLPTIATVQLTKVKEFTLKKTFAIENKPLKRKAKKYSRFFYEMVNDQESMPNNTTMD-RNEIGRVEKQ--RPKKSV-GSSMLNYIDKSSL---YDQVLKFYNKD---------KGFRNSIKKMKKLRREGKKIRRSRD--------------CLVGPWSAWSPCSVTCGIGEEVRRRKVTRKSRGGGRRCPKLKEV 864          

HSP 2 Score: 37.7354 bits (86), Expect = 1.029e-1
Identity = 25/72 (34.72%), Postives = 36/72 (50.00%), Query Frame = 0

Query:  497 IMMLNKKKDMQKEMKNMQN--DEG--ISRS-DCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSK 563
            +   NK K  +  +K M+    EG  I RS DC V  WS WS C+ +CG G   + R +  +S   G++  K
Sbjct:   85 LKFYNKDKGFRNSIKKMKKLRREGKKIRRSRDCLVGPWSAWSPCSVTCGIGEEVRRRKVTRKSRGGGRRCPK 300          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592923680|gb|GAXK01034735.1| (TSA: Calanus finmarchicus comp111761_c3_seq2 transcribed RNA sequence)

HSP 1 Score: 79.7221 bits (195), Expect = 2.489e-15
Identity = 49/169 (28.99%), Postives = 86/169 (50.89%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKS 280
            R +  G  A +G++Q  + G    L+ E  A ++ +   I        +  G T      D  N  VS+++ + PSPDW +G+  L LC+ N  +++      +P DAG D+G ++ SP+ PT+P+  I +ISS++P  P   F+      +  LA + + ++R Y+ S
Sbjct:  460 RLFSVGAIAGEGVKQFVETGDSDKLDRE-AATNNFLDAFIAPP---IASGGGNTSTTIFVDGNNTKVSMLTKLVPSPDWFIGLDSLNLCQDNH-FIDSITTEAFPLDAGTDNGFTFTSPNWPTDPQGVIFQISSQYPTHPAGSFHYPHLDKLPTLAIIQITKEREYQLS 951          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592923681|gb|GAXK01034734.1| (TSA: Calanus finmarchicus comp111761_c3_seq1 transcribed RNA sequence)

HSP 1 Score: 78.9518 bits (193), Expect = 1.430e-14
Identity = 52/179 (29.05%), Postives = 88/179 (49.16%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESELKAES---DHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFL 287
            R +  G  A +G++Q  + G    L+ E    +     I   I + G       G T      D  N  VS+++ + PSPDW +G+  L LC+ N  +++      +P DAG D+G ++ SP+ PT+P+  I +ISS++P  P   F+      +  LA + + ++R Y+   SDE  +
Sbjct:  460 RLFSVGAIAGEGVKQFVETGDSDKLDREAATNNFLDAFIAPPIASGG-------GNTSTTIFVDGNNTKVSMLTKLVPSPDWFIGLDSLNLCQDN-HFIDSITTEAFPLDAGTDNGFTFTSPNWPTDPQGVIFQISSQYPTHPAGSFHYPHLDKLPTLAIIQITKEREYQ--LSDEILI 966          

HSP 2 Score: 37.3502 bits (85), Expect = 1.466e-1
Identity = 13/25 (52.00%), Postives = 18/25 (72.00%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTR 340
            C+VS W  WS+CS++CG G  +R R
Sbjct: 1399 CKVSHWGSWSSCSLSCGIGEQVRVR 1473          

HSP 3 Score: 36.1946 bits (82), Expect = 3.287e-1
Identity = 16/37 (43.24%), Postives = 20/37 (54.05%), Query Frame = 0

Query:  523 DCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGK 559
            DC V+ W  WS C+ SCG G + + R I     S GK
Sbjct: 1396 DCKVSHWGSWSSCSLSCGIGEQVRVRKIEKHPKSRGK 1506          

HSP 4 Score: 33.8834 bits (76), Expect = 2.083e+0
Identity = 18/57 (31.58%), Postives = 27/57 (47.37%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRF--FNRMGRKKCPHIDTIEKLMCAGNTADCS 444
            C  S W  WS CS +CG G + R R+     +   K CP    + ++   G+  DC+
Sbjct: 1399 CKVSHWGSWSSCSLSCGIGEQVRVRKIEKHPKSRGKPCP---PLREVKWCGSARDCN 1560          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592777083|gb|GAXK01177485.1| (TSA: Calanus finmarchicus comp461011_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 74.3294 bits (181), Expect = 4.862e-13
Identity = 45/146 (30.82%), Postives = 77/146 (52.74%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKA---RGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPF 255
             +  G   S+G+ Q A+ GS   L+ E  A    + TII     +G+      G+T      D  + +VS+++ + PSPDW +G+  L+LC +   + +   +  +P DAG D+G ++ SP+  TEP+  + R++S+FP  P   F
Sbjct: 1033 LFSIGSTVSEGVRQFAETGSTDTLDQE-AANISFLDTIISPPIEQGV------GETSTNIFVDTNHTMVSVMTKIVPSPDWFIGLDSLDLCSQGA-FRQSVSIEAFPLDAGTDNGFTFTSPNWATEPRGEVFRMTSQFPAHPAGSF 1446          

HSP 2 Score: 35.039 bits (79), Expect = 7.119e-1
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTR 340
            C+VS WS W  C+ TCG G S R+R
Sbjct:  310 CKVSAWSAWGPCTKTCGIGESSRSR 384          

HSP 3 Score: 33.8834 bits (76), Expect = 1.996e+0
Identity = 15/41 (36.59%), Postives = 21/41 (51.22%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRF--FNRMGRKKCPHI 428
            C  S W+ W  C+ TCG G  SR+R      + G + CP +
Sbjct:  262 CKVSAWSAWGPCTKTCGIGESSRSRLISRHPQHGGQPCPAL 384          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592777084|gb|GAXK01177484.1| (TSA: Calanus finmarchicus comp461011_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 73.559 bits (179), Expect = 7.508e-13
Identity = 45/146 (30.82%), Postives = 77/146 (52.74%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKA---RGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPF 255
             +  G   S+G+ Q A+ GS   L+ E  A    + TII     +G+      G+T      D  + +VS+++ + PSPDW +G+  L+LC +   + +   +  +P DAG D+G ++ SP+  TEP+  + R++S+FP  P   F
Sbjct: 1033 LFSIGSTVSEGVRQFAETGSTDTLDQEA-ANISFLDTIISPPIEQGV------GETSTNIFVDTNHTMVSVMTKIVPSPDWFIGLDSLDLCSQGA-FRQSVSIEAFPLDAGTDNGFTFTSPNWATEPRGEVFRMTSQFPAHPAGSF 1446          

HSP 2 Score: 35.4242 bits (80), Expect = 5.917e-1
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTR 340
            C+VS WS W  C+ TCG G S R+R
Sbjct:  310 CKVSAWSAWGPCTKTCGIGESSRSR 384          

HSP 3 Score: 33.8834 bits (76), Expect = 2.092e+0
Identity = 15/41 (36.59%), Postives = 21/41 (51.22%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRF--FNRMGRKKCPHI 428
            C  S W+ W  C+ TCG G  SR+R      + G + CP +
Sbjct:  262 CKVSAWSAWGPCTKTCGIGESSRSRLISRHPQHGGQPCPAL 384          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592777082|gb|GAXK01177486.1| (TSA: Calanus finmarchicus comp461011_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 72.7886 bits (177), Expect = 1.038e-12
Identity = 48/161 (29.81%), Postives = 82/161 (50.93%), Query Frame = 0

Query:  120 ASKGLEQVAKWGSPRILESELKAESDHIRTIIKA---RGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLY 277
             S+G+ Q A+ GS   L+ E  A    + TII     +G+      G+T      D  + +VS+++ + PSPDW +G+  L+LC +   + +   +  +P DAG D+G ++ SP+  TEP+  + R++S+FP  P   F       +  +A   L + R Y
Sbjct:  958 VSEGVRQFAETGSTDTLDQEA-ANISFLDTIISPPIEQGV------GETSTNIFVDTNHTMVSVMTKIVPSPDWFIGLDSLDLCSQGA-FRQSVSIEAFPLDAGTDNGFTFTSPNWATEPRGEVFRMTSQFPAHPAGSFNYPHLDQLPTIAVFQLTKLREY 1416          

HSP 2 Score: 35.4242 bits (80), Expect = 5.651e-1
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTR 340
            C+VS WS W  C+ TCG G S R+R
Sbjct:  310 CKVSAWSAWGPCTKTCGIGESSRSR 384          

HSP 3 Score: 33.4982 bits (75), Expect = 2.058e+0
Identity = 15/41 (36.59%), Postives = 21/41 (51.22%), Query Frame = 0

Query:  390 CATSEWTKWSECSTTCGKGFRSRTRRF--FNRMGRKKCPHI 428
            C  S W+ W  C+ TCG G  SR+R      + G + CP +
Sbjct:  262 CKVSAWSAWGPCTKTCGIGESSRSRLISRHPQHGGQPCPAL 384          

BLAST of EMLSAG00000011205 vs. C. finmarchicus
Match: gi|592893145|gb|GAXK01065230.1| (TSA: Calanus finmarchicus comp709760_c2_seq4 transcribed RNA sequence)

HSP 1 Score: 70.8626 bits (172), Expect = 1.050e-12
Identity = 33/94 (35.11%), Postives = 57/94 (60.64%), Query Frame = 0

Query:  162 EGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPF 255
            EG+T A+   D  N  +S ++ + PSPDW  G+  L+LC ++  W++   + + P DAG D+G+++ SP+  T+P+  +  I+S FP+ P   F
Sbjct:  318 EGRTEALVFADGNNTKLSCITAIVPSPDWFTGLDDLDLC-QDGEWLDNARIEVSPMDAGTDNGLTFTSPNWATDPRGEVFAITSSFPDHPAASF 596          

BLAST of EMLSAG00000011205 vs. L. salmonis peptides
Match: EMLSAP00000011205 (pep:novel supercontig:LSalAtl2s:LSalAtl2s763:115555:179178:-1 gene:EMLSAG00000011205 transcript:EMLSAT00000011205 description:"maker-LSalAtl2s763-snap-gene-1.17")

HSP 1 Score: 1242.64 bits (3214), Expect = 0.000e+0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query:    1 MVIYEFSLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRLTSLSKQMHGWILERLEHMFWMVWY 597
            MVIYEFSLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRLTSLSKQMHGWILERLEHMFWMVWY
Sbjct:    1 MVIYEFSLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRLTSLSKQMHGWILERLEHMFWMVWY 597          

BLAST of EMLSAG00000011205 vs. L. salmonis peptides
Match: EMLSAP00000005758 (pep:novel supercontig:LSalAtl2s:LSalAtl2s30:197499:241515:1 gene:EMLSAG00000005758 transcript:EMLSAT00000005758 description:"maker-LSalAtl2s30-augustus-gene-2.5")

HSP 1 Score: 76.6406 bits (187), Expect = 5.514e-15
Identity = 56/195 (28.72%), Postives = 93/195 (47.69%), Query Frame = 0

Query:   92 WMAPPSGSGCVNFKTTVVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDF 286
            W  P   S  + F     +   +  G  AS  +++ A+ G    L+   K     I  +  A  +  P   G+T      D  +  VSL+S + PSPDW +G+  L+LC K+  +++   +   P DAG D+G ++ SP+ PTEP+  I +I++ +P+ P   F       +  +A   L ++R Y+ S   EDF
Sbjct:   64 WRPPAQWSKVIGFSHND-DYNLFHIGSIASDSVKEFAETGXTESLD---KVSDSLILDVFSAPPI--PKGAGRTQGSIFLDGNHTQVSLMSKLVPSPDWFIGLDALDLC-KDGRFIDSIVIESDPIDAGSDNGFTFTSPNWPTEPRAEIFKITNVYPSHPAGSFNYPELSSLPTIATFSLIKEREYEMS---EDF 248          

BLAST of EMLSAG00000011205 vs. L. salmonis peptides
Match: EMLSAP00000001943 (pep:novel supercontig:LSalAtl2s:LSalAtl2s133:467280:542329:-1 gene:EMLSAG00000001943 transcript:EMLSAT00000001943 description:"maker-LSalAtl2s133-augustus-gene-4.14")

HSP 1 Score: 72.4034 bits (176), Expect = 2.203e-13
Identity = 67/257 (26.07%), Postives = 103/257 (40.08%), Query Frame = 0

Query:  307 VEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIP---GKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTS-WSPWSQCNASCGKGFRRKFRTILVQSSSHGK 559
              ED+  P    ++W++WSTCSVTCG G   R RD L P     +++ GCD    + + C+  V KC                      +EW+ WSECS TCG G R   +R                E +          + N E D  C    + T + ++ I +        E         +    +    + +K ++      ++   E  +  DC V S W+ W++C+ SCGKG R K +  L  +S  GK
Sbjct: 1007 CNEDQPCP--SWTQWTEWSTCSVTCGGGNQKRIRDCLTPRNLNGSNIYGCDGDTWEMKACN--VRKCP-------------------QWAEWSSWSECSVTCGSGGRRNRKRECKEF------QFSFNEDV----------VVNMEKDLFCPGRYSQTQSCNNGIKNCPFPGEWSEWSTCSKTCGKGTKKRNRKCIDSKFRNGNPCNADLNETENCNEQDCPVWSPWTEWTECSRSCGKGMRSKMKICLDLTSCSGK 1224          

HSP 2 Score: 59.3066 bits (142), Expect = 2.321e-9
Identity = 82/318 (25.79%), Postives = 127/318 (39.94%), Query Frame = 0

Query:  249 NDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCAT-SEWTKWSECSTTCGKGFRSRTR-----RFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNE-----ECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDM---QKEMKNMQNDEGISRSDCSVTS-WSPWSQCNASCGKGFRRKFRTIL 551
            N  R PF+   G            RQ+ Y++  +D+  L ED +     G S EE+   +E   I     ++WSDW++CSV+CG G   RT+       A     D   ++KEMC+ + P                     C + ++WT+WS CS TCG G + R R     R  N      C   DT E   C  N   C    E     EC   C S                       ++ ++   K+ +    E +++N +KD+    +  +    + GI   +C     WS WS C+ +CGKG +++ R  +
Sbjct:  905 NKQRCPFWSSWGEWTSCSQSCNGGRQKRYRECLNDDRSLGEDCN-----GDSYEEKACNKETCPI----YNKWSDWTSCSVSCGSG-GXRTKIRTCKRDAPQSCKDE--IKKEMCNEDQP---------------------CPSWTQWTEWSTCSVTCGGGNQKRIRDCLTPRNLNGSNIYGCDG-DTWEMKAC--NVRKCPQWAEWSSWSECSVTCGSGG---------------------RRNRKRECKEFQFSFNEDVVVNMEKDLFCPGRYSQTQSCNNGIK--NCPFPGEWSEWSTCSKTCGKGTKKRNRKCI 1163          

HSP 3 Score: 58.151 bits (139), Expect = 6.088e-9
Identity = 59/252 (23.41%), Postives = 100/252 (39.68%), Query Frame = 0

Query:  318 VSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSE-VPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTAD--CSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVT--------SWSPWSQCNASCGKGFRRKFRTILVQSSSHG 558
            ++ W++W+ CS++CG G   R RD L+     +  C   L + + C+SE  PK                       SEWT+W+ CS +CG G ++R R   +R         D  EK  C+ N      S  N        S+ +++  G + I   + ++  +  + K   +    + + E    N+K+    +        G+    C+           W  WS C+ASCG G + + R  L     +G
Sbjct: 1239 LTPWTEWTPCSLSCGGGXQRRERDCLVSRSGFLNPCFEPLEEIQRCNSESCPK----------------------WSEWTEWTPCSESCGGGTQTRVRDCVSRSACNSLGDSDK-EKRECSVNACPSWTSWSNW-------SVCSASCGGGTRI---RNRECXLNARNKAPCIGD-SVQREECGRRNRKRKCSSDQSKDCTGSGLISEPCNENKDCPSGWXEWGQWSSCSASCGGGSQLRKRECLSPGGRYG 1456          

HSP 4 Score: 58.151 bits (139), Expect = 6.408e-9
Identity = 65/284 (22.89%), Postives = 101/284 (35.56%), Query Frame = 0

Query:  320 EWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTR------------RFFNRMGRKKCPHID-----TIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMK--------NMQNDEGISRSDCSV------TSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMR 572
            EWS+WSTCS TCGKG   R R  +     +   C+  L + E C+ +                     D    S WT+W+ECS +CGKG RS+ +            ++        CP +      T   L C G                +   +   +   F+    +    +++   E   K  E  +      +     Q  ++        N   D    + +CSV      TSWS WS C+ASCG G R + R   + + +    +     +E   R
Sbjct: 1140 EWSEWSTCSKTCGKGTKKRNRKCIDSKFRNGNPCNADLNETENCNEQ---------------------DCPVWSPWTEWTECSRSCGKGMRSKMKICLDLTSCSGKTKYMELCNENPCPSLTPWTEWTPCSLSCGGGXQ-------------RRERDCLVSRSGFLNPCFEPLEEIQRCNSESCPKWSEWTEWTPCSESCGGGTQTRVRDCVSRSACNSLGDSDKEKRECSVNACPSWTSWSNWSVCSASCGGGTRIRNRECXLNARNKAPCIGDSVQREECGR 1389          

HSP 5 Score: 56.225 bits (134), Expect = 2.133e-8
Identity = 49/139 (35.25%), Postives = 57/139 (41.01%), Query Frame = 0

Query:  320 EWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCAT--SEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKS 456
            EW  WS+CS +CG G  +R R+ L PG                  SE P C G S         F   D C T  SEWT WS CS+TCG G + RTR                   + C GN  + SL N   DR C S
Sbjct: 1426 EWGQWSSCSASCGGGSQLRKRECLSPGGR----------YGPFLESEGP-CPGPSEITL-----FCNLDDCPTVWSEWTPWSSCSSTCGGGSKKRTR---------------VCSGMQCEGNQLETSLCN---DRPCYS 1530          

HSP 6 Score: 56.225 bits (134), Expect = 2.424e-8
Identity = 58/235 (24.68%), Postives = 87/235 (37.02%), Query Frame = 0

Query:  321 WSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDC-SVTSWSPWSQCNASCGKGFRRKFRTILVQS 554
            WS+WS CS TCG+G+  R R+                     CS +   CEG+S       G+  P      +EW+ W+ECS TCG G + + R      G +          L+C G              I K  NN          D           R+  + +         + + +  +    +K +   E  +   C S + WS WS+C+ SCG G+R+K R     S
Sbjct:  700 WSEWSACSTTCGEGIQKRKRE---------------------CSGDF--CEGASIETKKCSGNVCP----VWTEWSDWTECSVTCGGGKKXKVRECVLPSGERS-------RGLICPGE-----------GYISKKCNNYPCPELGPWSDWSSCSSSCGSGRRTRIRECG-------LPITRGSNNNPCLKPLFEIEVCNPQKCPSFSPWSEWSECSVSCGGGYRKKSRECKASS 882          

BLAST of EMLSAG00000011205 vs. L. salmonis peptides
Match: EMLSAP00000010397 (pep:novel supercontig:LSalAtl2s:LSalAtl2s68:326217:339785:1 gene:EMLSAG00000010397 transcript:EMLSAT00000010397 description:"maker-LSalAtl2s68-snap-gene-3.35")

HSP 1 Score: 51.9878 bits (123), Expect = 3.283e-7
Identity = 43/120 (35.83%), Postives = 56/120 (46.67%), Query Frame = 0

Query:  309 EDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFN---RMGRKKC 425
            ++E+ PG Q S WS WS+CS +CG G   R R     G+ S   C  Q  Q + C              AN   D         + WT+WS CST+CG GFRSR+R   +   R G  +C
Sbjct:  226 DNEAKPG-QWSLWSTWSSCSKSCGDGEQRRDRT-CGNGERSQQNCVGQSSQGQSC------------VIANCKIDG------EWARWTRWSPCSTSCGTGFRSRSRACSDPIPRYGGNRC 325          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783479|sp|Q9W770.1|SPON1_CHICK (RecName: Full=Spondin-1; AltName: Full=F-spondin; Flags: Precursor)

HSP 1 Score: 213.001 bits (541), Expect = 1.434e-58
Identity = 150/446 (33.63%), Postives = 218/446 (48.88%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++M+GPSPDW VG+S  +LC K+C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +K +A+++L R     + C   +F+P D      + ++ EE+   EED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  S+W  WS CS +CG G RSR R        K+ P   ++ K+     T  C + NEEC     +            S    MK++  M++    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  240 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTYESPNKPTVPQEKIRPLTSL--DHPQSPFYDPEGGSIKLVARVVLERIARKGEQC---NFVP-DNIDDIVADLAPEEK---EEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCMMSDWITWSPCSVSCGMGTRSRERYV------KQFPEDGSMCKVP-TEETEKC-IVNEECSPSSCLVTEWGEWDECSASCGTGMKRRHRMIKMTPADGSMCKAETTEAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 636          

HSP 2 Score: 83.1889 bits (204), Expect = 1.950e-15
Identity = 72/271 (26.57%), Postives = 118/271 (43.54%), Query Frame = 0

Query:  288 PEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTR--RFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSS 556
            P D SM  ++  ++ E+  + E  +IP C +S WS+WS CSVTCGKG  MRTR  ++   A +  C+ +L Q E C   +P+C               P D C  +EW++WSEC+T+CGKG   RTR  +   + G   CP  +T+++  C                                             R    ++   M K       +K+  ++  KN+ N++      C +  W+ W++C+  CG G + ++  +  +S S
Sbjct:  584 PADGSM-CKAETTEAEKCMMPECHTIP-CLLSPWSEWSDCSVTCGKG--MRTRQRMLKSAAELGDCNEELEQAEKCM--LPEC---------------PID-CELTEWSQWSECNTSCGKGHMIRTRMIKIEPQFGGTACP--ETVQRTKC---------------------------------------------RVRKCLRGPGMEKRRWKEAREKRRSEQAKKNIDNEQ---YPVCRLKPWTAWTECSTLCGGGIQERYMMVKKRSKS 782          

HSP 3 Score: 58.151 bits (139), Expect = 1.397e-7
Identity = 32/106 (30.19%), Postives = 54/106 (50.94%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W APP+G+GCV  K ++V++R   F + G    +  EQ
Sbjct:   85 FRGFTLIALKEGKEGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICEQ 179          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783463|sp|Q8VCC9.1|SPON1_MOUSE (RecName: Full=Spondin-1; AltName: Full=F-spondin; Flags: Precursor)

HSP 1 Score: 212.231 bits (539), Expect = 2.413e-58
Identity = 148/446 (33.18%), Postives = 217/446 (48.65%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  ++    T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSVSCGMGMRSRERYV------KQFPEDGSV-CMLPTEETEKCTV-NEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 82.8037 bits (203), Expect = 3.356e-15
Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C V+EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++  EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K    K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSVCMLP-TEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSC---------GKGHMIRTRTIQMEPQFGGVPCPETVQRKKCRTRKCLRSPSVQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.4622 bits (145), Expect = 2.420e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENQEGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|544353|sp|P35446.1|SPON1_RAT (RecName: Full=Spondin-1; AltName: Full=F-spondin; Flags: Precursor)

HSP 1 Score: 212.231 bits (539), Expect = 2.799e-58
Identity = 148/446 (33.18%), Postives = 217/446 (48.65%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  ++    T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPSWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSVSCGMGMRSRERYV------KQFPEDGSV-CMLPTEETEKCTV-NEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 82.4185 bits (202), Expect = 3.958e-15
Identity = 73/280 (26.07%), Postives = 116/280 (41.43%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C V+EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++  EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K    K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSVCMLP-TEETEKCTVNEECSPSSCLVTEWGEWDDCSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQAEKCMLPECPIDCELSEWSQWSECNKSC---------GKGHMIRTRTIQMEPQFGGAPCPETVQRKKCRARKCLRSPSIQKLRWREARESRRSEQLREESDGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 3.357e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783472|sp|Q9HCB6.2|SPON1_HUMAN (RecName: Full=Spondin-1; AltName: Full=F-spondin; AltName: Full=Vascular smooth muscle cell growth-promoting factor; Flags: Precursor)

HSP 1 Score: 211.846 bits (538), Expect = 3.657e-58
Identity = 148/446 (33.18%), Postives = 216/446 (48.43%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  L     T  C++ NEEC     +            +  + MKK+  M++    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSISCGMGMRSRERYV------KQFPEDGSVCTLP-TEETEKCTV-NEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSPWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 84.7297 bits (208), Expect = 8.354e-16
Identity = 73/280 (26.07%), Postives = 118/280 (42.14%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH-IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C ++EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++ +EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K  + K       + +   + ++ ++  +  +  EG     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSV-CTLPTEETEKCTVNEECSPSSCLMTEWGEWDECSATCGMGMKKRHRMIKMNPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNKSC---------GKGHVIRTRMIQMEPQFGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRSEQLKEESEGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 3.886e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALRENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783471|sp|Q9GLX9.1|SPON1_BOVIN (RecName: Full=Spondin-1; AltName: Full=F-spondin; AltName: Full=Vascular smooth muscle cell growth-promoting factor; Flags: Precursor)

HSP 1 Score: 211.46 bits (537), Expect = 4.970e-58
Identity = 149/446 (33.41%), Postives = 216/446 (48.43%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             WEYGGYAS+G++QVA+ GSP  +E E++ +SD + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG+S  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ PFYD  G  +  +A++++ R     + C   + +P D      + ++ EE+   +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  Q  M     P C    G              C  SEW  WS CS +CG G RSR R        K+ P   ++  L     T  C++ NEEC     +            +  + MKK+  MV+    +  M + E  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  245 LWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSL--DHPQSPFYDPEGGSITQVARVVIERIARKGEQC---NIVP-DNVDDIVADLAPEEK---DEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCM----GPGCSDEDG------------STCTMSEWITWSPCSISCGTGTRSRERYV------KQFPEDGSVCTLP-TEETEKCTV-NEECSPSSCLTTEWGEWDECSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTI----------------PCLLSLWSEWSDCSVTCGKGMRTRQRML 641          

HSP 2 Score: 81.6481 bits (200), Expect = 6.326e-15
Identity = 72/280 (25.71%), Postives = 117/280 (41.79%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKC-PHIDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMMVE----------QQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+       + E+  V E+ S   C  +EW +W  CS TCG G+  R R   + P   SM  C  +  Q E C   +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C   ++ +EK M      DC L       EC++ C         G   +I  +  QM  +          Q++K  + K       + +   + ++ ++  +  +  +G     C +  W+ WS+C   CG G + ++ T+
Sbjct:  531 FPEDGSV-CTLPTEETEKCTVNEECSPSSCLTTEWGEWDECSATCGMGMKKRHRMVKMSPADGSM--CKAETSQAEKCM--MPECH------------TIP---CLLSLWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEELEQVEKCMLPECPIDCELTEWSQWSECNKSC---------GKGHMIRTRMIQMEPQFGGTPCPETVQRKKCRIRKCLRNPSIQNLRWREARESRRSEQLREESDGDQFPGCRMRPWTAWSECTKLCGGGIQERYMTV 781          

HSP 3 Score: 60.077 bits (144), Expect = 3.596e-8
Identity = 33/106 (31.13%), Postives = 55/106 (51.89%), Query Frame = 0

Query:   24 FREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQ 126
            FR F L+   +  +G    D             G+FQ++ +  T+F  +C   VT+++   ++ IQV W+APP+G+GCV  K ++V++R   F + G    K  EQ
Sbjct:   90 FRGFTLIALKENREGDKEED-----------HAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQ 184          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|544354|sp|P35447.1|SPON1_XENLA (RecName: Full=Spondin-1; AltName: Full=F-spondin; Flags: Precursor)

HSP 1 Score: 183.726 bits (465), Expect = 2.398e-48
Identity = 138/449 (30.73%), Postives = 210/449 (46.77%), Query Frame = 0

Query:  110 EERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYP----NHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTC-SVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR---ICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
            E   WEYG  AS G++QVA+ GSP  +E E++ + D + T+IKA+  W      N      A F  D+  HL+S ++MMGPSPDW VG++  +LC K C WV++    L PWDAG DSG++YESP+ PT P+  I  ++S   + P+ P     G ++ P+A++++ R     + C   + +P++        V++E+    +ED++   C  S WS WS C S TC KG  MR R        S+   D Q  +  M     P C                   C  SEW  WS CS +CG G   R R        K+ P   ++ K+     T  C + NEEC+    I            +  + MKK+  M++    +  M + +  + E  M+ +   +                 C ++ WS WS C+ +CGKG R + R +
Sbjct:  238 EYVLWEYG-QASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLTSEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQDKIRPLTSL--DHPQSPSMTRGGPII-PIARVVIERIARKGEQC---NIIPDNVDDIVADLVTEEK----DEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCM----GPGCSDDEA------------STCMMSEWITWSPCSASCGMGIEVRERYV------KQFPEDGSLCKVP-TEETEKC-IVNEECEPSSCIVTEWAEWEECSATCRMGMKKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTI----------------PCVLSPWSEWSDCSVTCGKGTRTRQRML 635          

HSP 2 Score: 80.8777 bits (198), Expect = 1.178e-14
Identity = 74/281 (26.33%), Postives = 115/281 (40.93%), Query Frame = 0

Query:  287 LPEDESMHFESGVSKEEEQGVEEDESIPG-CQVSEWSDWSTCSVTCGKGLSMRTRDF-LIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPH---IDTIEKLMCAGNTADCSLDN----EECDRICKSLNNSTSAGHSFIIDMKKKQMM--------VEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             PED S+      ++E E+ +  +E  P  C V+EW++W  CS TC  G+  R R   + P   SM   D   V+K M    +P+C              +P   C  S W++WS+CS TCGKG R+R R   +      C     +  +EK M       C L       EC       N S   GH     M   +            Q++K  + K  +    E   L   ++ ++  K  ++ +G     C +  W+ W++C   CG G + +F T+
Sbjct:  525 FPEDGSLC--KVPTEETEKCIVNEECEPSSCIVTEWAEWEECSATCRMGMKKRHRMIKMTPADGSMCKADTTEVEKCM----MPECHT------------IP---CVLSPWSEWSDCSVTCGKGTRTRQRMLKSPSELGDCNEELELKQVEKCMLPECPISCELTEWSYWSEC-------NKSCGKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRHLKDAREKRRSEKIKEDSDGEQYPVCKMKPWTAWTECTKFCGGGIQERFMTV 777          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|313104285|sp|Q9BUD6.3|SPON2_HUMAN (RecName: Full=Spondin-2; AltName: Full=Differentially expressed in cancerous and non-cancerous lung cells 1; Short=DIL-1; AltName: Full=Mindin; Flags: Precursor)

HSP 1 Score: 114.39 bits (285), Expect = 9.671e-27
Identity = 75/233 (32.19%), Postives = 115/233 (49.36%), Query Frame = 0

Query:  114 WEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKA-RGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIP----GCQVSEWSDWSTCSVTCGK-GLSMRTR 340
            W    Y S GL   A+ G    L  E++A  + ++++ +       P+  G+T A     +++ LVS V  + PSPDW VGV  L+LC  +  W E+  L LYP+DAG DSG ++ SP+  T P+  +T I+S  P+ P   FY      + P+A++ L R R   ++     F+P        + V    +  + +  S+P     C+VS WS W  C   CG+ G   RTR
Sbjct:   84 WRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRA-----FIP-------PAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTR 303          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783424|sp|Q9WV75.1|SPON2_RAT (RecName: Full=Spondin-2; AltName: Full=Mindin; Flags: Precursor)

HSP 1 Score: 106.686 bits (265), Expect = 4.912e-24
Identity = 73/229 (31.88%), Postives = 107/229 (46.72%), Query Frame = 0

Query:  114 WEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKA-RGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGK-GLSMRTR 340
            W    Y S GL   A+ G    L  E++A  + ++++         P+  G+T A      ++ LVS V  + PSPDW VG+  L+LC     W E+  L LYP DAG DSG ++ SP+  T P+  +T I++  P+ P   FY      + P+AK+   R R   ++     F P    +   +    E    +   E+   C+VS WS W  C   CGK G   RTR
Sbjct:   83 WRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAVPSGTGQTSAELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGG-RWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLRQSPRA-----FAPPSLDL---ASRGNEIVDSLSVPETPLDCEVSLWSSWGLCGGPCGKLGAKSRTR 302          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|52783454|sp|Q8BMS2.2|SPON2_MOUSE (RecName: Full=Spondin-2; AltName: Full=Mindin; Flags: Precursor)

HSP 1 Score: 102.834 bits (255), Expect = 8.297e-23
Identity = 73/231 (31.60%), Postives = 107/231 (46.32%), Query Frame = 0

Query:  114 WEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTI---IKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGK-GLSMRTR 340
            W    Y S GL   A+ G    L  E++A  + ++++     A  +  P+  G+T        ++ LVS V  + PSPDW VG+  L+LC     W E+  L LYP DAG DSG ++ SP+  T P+  +T I++  P+ P   FY      + P+AK+   R +      S   F P    +   +    E    +   E+   C+VS WS W  C   CGK G   RTR
Sbjct:   83 WRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAI--PSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGG-RWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQQ-----SPRAFAPPSLDL---ASRGNEIVDSLSVPETPLDCEVSLWSSWGLCGGPCGKLGAKSRTR 302          

BLAST of EMLSAG00000011205 vs. SwissProt
Match: gi|81864798|sp|Q700K0.1|SSPO_RAT (RecName: Full=SCO-spondin; Flags: Precursor)

HSP 1 Score: 73.9442 bits (180), Expect = 2.939e-12
Identity = 76/349 (21.78%), Postives = 133/349 (38.11%), Query Frame = 0

Query:  265 PLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFL---IPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFN---RMGRKKCP---HIDTIEKL-MCAGNTADCS-----LDNEECDR---------ICKSLNNSTSAGHSFIIDMKKKQMMVEQQR------KELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRLTSLSKQMHGW 583
            P ++ + +  +L   SC   + LP +     +       + G +ED+ +  C ++ WS WS CS +CG GL  + R+ L   +PG + ++    QL  +       P     +G +A               +W  W  CS +CG G +SR R   +   + G   CP   H   +  L +C G+T DC      ++ E C +               N + +GH          + ++            ++ Q  M      +L+       E   +          C  ++WSPW+ C+ SCG G R +FR+     ++ G    +   +E +   T     + GW
Sbjct: 2443 PASRALCSPSQL---SCGSGECLPLEHRCDLQVNC----QDGSDEDDCV-DCVLAPWSGWSDCSRSCGLGLIFQHRELLRPPLPGGSCLL---DQLRSQPCFVQACPV----AGAWA---------------KWGPWEPCSVSCGGGHQSRQRSCVDPPPKNGGAPCPGPSHEKVLCNLQLCPGDT-DCEPGRVHVNAELCQKGLLPPCPPSCLDPEANRSCSGHCVEGCRCPPGLFLQDSHCLPLSECPCLVGQKLMQPGLAFLLDNCSQCICESGILLCKPAACSQSCGWSAWSPWTACDHSCGSGVRARFRSPTNPPAAFGGSPCEGDRQELQACYTDCGTDIPGW 2760          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: EFX77761.1 (hypothetical protein DAPPUDRAFT_321105 [Daphnia pulex])

HSP 1 Score: 382.104 bits (980), Expect = 2.549e-122
Identity = 197/335 (58.81%), Postives = 241/335 (71.94%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD AG  MKPLA++++ RQR+Y+K+C+D   + ED        VS  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  235 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPAGSDMKPLARVVITRQRVYEKACTDTSTITED-------AVSHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDICAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMKFEC 549          

HSP 2 Score: 83.9593 bits (206), Expect = 5.642e-16
Identity = 46/103 (44.66%), Postives = 61/103 (59.22%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            + L G ++Q + +KF  FLL   P         +   P S D +G   SF+L  D L+RFSD C+N +TQTSSV K+EIQVLW AP  G+GC+ F   V+E R
Sbjct:   68 VTLQGWRSQNVLQKFIGFLLTATPS--------NPAIPTSPDTAG---SFRLFSDSLSRFSDVCSNGITQTSSVPKTEIQVLWTAPGPGTGCIKFSAVVLENR 159          

HSP 3 Score: 68.9366 bits (167), Expect = 2.927e-11
Identity = 60/206 (29.13%), Postives = 91/206 (44.17%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D IC         + S + G  F    ++    + +++  L   + EM             M+ E ++   DE     DC V+ WS WS C+A+CGKG + + R  LV
Sbjct:  399 RQRVYEKACTDTSTITEDAVSHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDICAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMKFECEDSGFDES-DDPDCLVSPWSDWSPCSATCGKGMQVRSRIPLV 590          

HSP 4 Score: 56.225 bits (134), Expect = 3.030e-7
Identity = 29/77 (37.66%), Postives = 43/77 (55.84%), Query Frame = 0

Query:  303 EEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMG------CDRQLVQKEMCSSEVPKCEGS 373
            E+ G +E +  P C VS WSDWS CS TCGKG+ +R+R  L+ G   +        C+   ++K +C ++ P C  S
Sbjct:  550 EDSGFDESDD-PDCLVSPWSDWSPCSATCGKGMQVRSRIPLVIGNNGVAATSLPAHCNMDTMEKTVCMADKPDCSFS 625          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: gb|EFA01633.2| (Spondin-1-like Protein [Tribolium castaneum])

HSP 1 Score: 352.829 bits (904), Expect = 2.940e-110
Identity = 247/736 (33.56%), Postives = 341/736 (46.33%), Query Frame = 0

Query:    5 EFSLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTV---------------------------------------------------VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSS-------------EVPKC--------------------------------EGSSGFYANAP---------GDFLPDDM------CATSEWTKWSECSTTCGKGFRSRTRRFFNRMG------------------RKKCPHIDTIEKLMCAGNTAD-----------CSL--DNEEC---------DRI---CKSLNNSTSAGHS-FIIDMKKKQMMVEQQRKELVM------KQHEMMKTEIMMLNKKKDM----QKEMKNMQNDEGISRSDCSVTSWSPWSQCNAS--CGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRL 573
            ++++ +R   D+      KF EF+L V+ ++           PPS+         QL GD LT+FS+ C++TV  T S  KSEIQVLW+AP SG+GCV FK  V                                                   V   FW YG  AS+GL Q+A+ G+ R+LESELKA+SDHIRTIIKARG+ YPN  GKTFAVFR D ++HL+S+VSM+ PSPDWIVGVS LELC +NC+WVE K L LYPWDAG D GI+Y SP+ P+ P+ PI RI++  P+DPR PFYD +G  MKPLA+L L+RQRLY+K C        D S+             VE+ +   GC+  EWSDWS CS TCG+G+  + R +    + S   C ++L ++  C +             E PKC                                +  +    N P         G+   DD+      C    W++WS CS TCGKGF+ R R                            C H    E + C   + +           CSL  D   C         D I   C+  + S   G+S     +++ Q +    +KEL+       +Q ++  + ++  N + D         +     + I+ +DC +T WS WS CN +  CG G+R K+R I V  +  GK  SK+  K+ K  +
Sbjct:  110 QYTVFLRATTDE------KFAEFMLSVENEKRKSP-------PPSI---------QLYGDSLTKFSEECSSTVISTDSQPKSEIQVLWLAPASGTGCVTFKGAVVISDGVWCGEAGSLTKTLCENSQDNDDIQPVILKHCCACDEANDIIGASHTVNYTFWRYGDIASEGLRQLAENGNTRMLESELKAKSDHIRTIIKARGISYPNITGKTFAVFRVDNRHHLMSIVSMIDPSPDWIVGVSSLELCLRNCSWVESKILNLYPWDAGTDDGITYISPNQPSSPRQPIKRITATSPSDPRSPFYDKSGAEMKPLARLYLSRQRLYEKLC--------DPSI-------------VEDYDDSDGCETEEWSDWSPCSATCGRGVKYKQRRY--KNEESKYVCHKKLTERAGCEAVQKYCPHQPVREVEDPKCVLSEWSEWSSCSVTCGKGTKTRDRKYKNRLAAKSCAAAKVNPPKLQENLECYGEGKCDDLEEEVVDCPQRPWSEWSPCSVTCGKGFKVRYRLSLQSQNWPFVPNSDSYDETEEFDEDDPCSHQKVKETVECFQKSCEQEEELPEKGYVCSLPKDVGACKSNTDRWYFDNIKGNCEIFSYSGCDGNSNNFKTLEQCQKVCADYQKELIANLTAHKRQLDVTISGVLSYNIQNDAINVSAPSYRPYSQIQLINSTDCVLTKWSSWSHCNVTGHCGSGYRTKYRQIKVHPTDGGKPCSKRLTKKKKCHV 800          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: gb|EFA01753.1| (Spondin-1-like Protein [Tribolium castaneum])

HSP 1 Score: 345.125 bits (884), Expect = 1.518e-108
Identity = 181/395 (45.82%), Postives = 255/395 (64.56%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDD---MCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEEC-DRICKSLN----NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMM 499
            FW  G  AS+G   +A+WGS R++E+EL+A+S ++RTIIKA GLWYPN    T A FR D+++HL+SL SM+GPSPDW+VGV+GL LC KNCTW+E   + L+P+DAG DSGISY SP++ T+P+  + RI++ +P DPR PFYD +   M PLA+L L R++L  K+C DE+FL      + +  VS+  E+      + P C V+E+S WS CSVTCGKGL MR+R++ +P KA M  C+RQL+ KEMC + VP+CEG +     +  D   ++   +CATS W  WSECS TCG GF+ RTR F + MGRKKCPHI  +EK  C   +  C+ D  E  D +C + +    +  SA     I M+ + ++VE   ++    + E+++    M
Sbjct:  222 FWGEGQIASEGFRSLAEWGSVRLMETELRAKSKYLRTIIKAAGLWYPNVNTNTSANFRVDRRHHLISLSSMLGPSPDWVVGVNGLNLCLKNCTWIENLIIDLFPYDAGTDSGISYMSPNAETKPRERMYRITTTYPEDPRAPFYDPSKKEMTPLARLYLKREKLIPKNC-DENFL------NAQLDVSENTEE-----INRPECAVTEYSQWSECSVTCGKGLRMRSREYRMPQKAKMFNCNRQLISKEMCVAAVPECEGGTPEEEESIEDIPLENIEGICATSPWGPWSECSATCGVGFKMRTRLFIDSMGRKKCPHITVVEKEKCM--SPPCTADQIEIPDPMCPTTDWSDWSPCSASCGKGIRMRTRLLLVEPGLQQKCSSRVELIQQAPCM 602          

HSP 2 Score: 65.0846 bits (157), Expect = 5.347e-10
Identity = 34/106 (32.08%), Postives = 58/106 (54.72%), Query Frame = 0

Query:    8 LHIRLDGDKTQFIERKFREFLLVVDP-QEDDGSSI-FDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEE 111
              IR++ +  +FI  K     L  +  Q     +I  ++   P   +  R GSFQL  D +++F++ C NT+++++   K+E   +W APP GSGCV FK  V+++
Sbjct:   39 FQIRINENPEKFIPGKVYTLYLTHNKLQHFKRFTINVEAASEPGNLSPQRVGSFQLFSDSISKFNEECVNTISESNDSPKTEAFFMWAAPPPGSGCVTFKAMVLQD 144          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: EEB20418.1 (f-spondin, putative [Pediculus humanus corporis])

HSP 1 Score: 341.658 bits (875), Expect = 8.380e-107
Identity = 185/426 (43.43%), Postives = 254/426 (59.62%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKC-EGSSGFYANAPGDFLPDD--------MCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLN----NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNK----KKDMQKEMKNMQNDEGISR 521
            FW  G  AS GL QVA+WGS R LE+EL++++ +++T+IKA GLW+P+    T A F+ DK+ HL+S+VSM GP+PDWIVGVSGL LC+++CTWVE K + LYP+DAG DSG+SY SP+ PT P+  I+RI+  +P DPR PFYD  G  MKPLAKL L    +  K C ++     D ++     V   E     ED   P C V++++ WS CSVTCGKG+ MRTR++L+P KA M+GCDRQLV KEMC ++ P C EG   F   +P  +LPD          C TS + +WSECSTTCG G   RTRRF +R GRKKCPH+  +EK  C              D  C +      +  SA     +  + +Q++V  + K++   + E+ +  I  L       +   K++  ++ D G  R
Sbjct:  249 FWGEGQIASDGLRQVAEWGSARNLEAELRSKARNLKTLIKAAGLWHPHVNENTTATFKVDKQKHLLSVVSMFGPTPDWIVGVSGLNLCQRDCTWVENKVIDLYPYDAGTDSGVSYMSPNEPTIPRERISRITPLYPEDPRAPFYDPQGKPMKPLAKLYLKLDYVTPKQCHED----VDGTVDLPPVVDLTENT---EDTRRPECNVTDYTPWSPCSVTCGKGIRMRTRNYLMPQKAQMLGCDRQLVSKEMCVADQPSCSEGP--FQYPSPFQYLPDSDEVEDTEAFCETSPFGEWSECSTTCGVGVAMRTRRFLDRWGRKKCPHVSLVEKRECIEPPCPSPSPGINMDSRCTTSEWTDWSPCSATCGTGLRYRSRQVLVGGELKDICAGRVELNQQRICTLQADCSVDRTSAKQICMLEPDSGPCR 665          

HSP 2 Score: 72.4034 bits (176), Expect = 2.322e-12
Identity = 54/162 (33.33%), Postives = 81/162 (50.00%), Query Frame = 0

Query:   14 GDKTQFIERKFREFLLVVD---PQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER---FWEYGGYASKGLEQVAKW--GSPRILESELKAES-DHIRTIIKARGLWYPNHEGKTF 166
            G   Q   +KF+ F+L V+   PQ D    IF  H     D+  R G+F L  D LT++   C NT+ ++    KSEIQV+W APP GSGCV F+  V+ ++   F +  G + K  E   ++  G+  +  + L+  S D  +  +   GLW  +   K F
Sbjct:   71 GRNHQGYLQKFKGFVLTVEASHPQSD----IF--HDTDGSDSPVRVGTFMLYSDELTQYHGECINTIKESDLSDKSEIQVMWQAPPKGSGCVTFRAMVLVDKDTWFADDEGLSKKICEITPEYLRGTEDVESNTLECCSCDEAKYTMVFEGLWSSSTHPKDF 226          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: XP_006559693.1 (PREDICTED: spondin-1-like isoform X8 [Apis mellifera])

HSP 1 Score: 334.724 bits (857), Expect = 1.618e-104
Identity = 185/445 (41.57%), Postives = 253/445 (56.85%), Query Frame = 0

Query:  108 VVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRS--DCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             V+ RFWEY G AS GL+QVA++G  R LESELK +SDHIRTIIKARG+ YPN  GKTFAVFR D+K+HL+SLVSM+ PSPDWIVGVSGLELC+ NC+W+E KEL LYP+DAG D+GI+Y SPDSPTEP+  I RI+S +PND R PFYD +G+ MKPLA+L LNRQRLY+K+C +   +P D +M+  +   K  ++          C+V+ W+ W  CSVTCG+G  +R R +     A+   C+  L  +  C  E+P C              L  + CA  +W+ WS CS TCG   ++R+R F  +   K+C  I                            L  + +  +    DM K ++            Q E  K      + ++D + E   M+  E   ++  +   T WS WS C+++CG G + +FR +
Sbjct:   71 TVDYRFWEYNGMASTGLQQVAEFGLTRKLESELKNQSDHIRTIIKARGISYPNVTGKTFAVFRVDQKHHLMSLVSMIDPSPDWIVGVSGLELCQSNCSWIEHKELNLYPYDAGTDNGITYLSPDSPTEPQEAIHRITSSYPNDSRSPFYDPSGLDMKPLARLYLNRQRLYEKTCEE---IPGD-AMNTGTPDKKRRDK---------ACKVTSWAPWEACSVTCGRGTRLRQRFYEDKAAATQNKCNVTLTSRAKCQGEIPHCNNRG-----RDAGILETEKCALHDWSTWSSCSATCGDSHKTRSRNFKYKKHLKECKSIPNG-----------------------PVLQETIACEYVQCPDMDKDEVSESPS-------QEEENKNNEQDNDNEEDYEGEAPVMEVTEKWQQNCPEERYTQWSLWSPCSSTCGPGIQLRFRLV 467          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: gb|KFM77843.1| (hypothetical protein X975_09445, partial [Stegodyphus mimosarum])

HSP 1 Score: 332.798 bits (852), Expect = 2.484e-104
Identity = 185/446 (41.48%), Postives = 252/446 (56.50%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEI--MMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSS 555
            R W+YGG AS+G+++VA+ G  + LESELK ES+ IRTIIKARGLWYPN +GKTFAVFR DK +HL+SL++M+GPSPDWIVGVS LELC KNC+WV EK + LYPWDAG  +G +Y  P  PT P+  I RI+S  PN+P  PFYD  G  MKP+A+L + RQR+Y+K C  ED+L            + +  +  ++  +   C+VS+W+D+  CSV+CG G  MR+R +    KA M GC  QLV KE C+   P C G+                C T+EW +WSECS TCGKG R+R+R++ +   RK C   + +EK  C    A C  +                             +   ++R E+  KQ+      +  ++LN + D+               S C+VT WS WS C  +CGKG + + R  L  S+
Sbjct:  154 RMWQYGGNASEGVKEVAERGITKKLESELKTESNKIRTIIKARGLWYPNVQGKTFAVFRVDKYHHLMSLLTMLGPSPDWIVGVSALELCLKNCSWVGEKVMNLYPWDAGTMNGYTYIDPKVPTIPREKIRRITSSTPNNPLSPFYDPTGAQMKPVARLTVTRQRVYEKPC--EDYL---------IPTTYDTTESTDDTYTRMECRVSDWTDFQPCSVSCGHGFKMRSRQYTNKKKAEMAGCTAQLVDKEPCN---PPCVGNIS--------------CRTTEWGEWSECSVTCGKGLRTRSRKYLDNRARKTC-AFELMEKETCMAPKAVCDTE----------------------------PIRPGRRRPEVSPKQNNASDDLLYRILLNVEMDLD------------PNSTCAVTQWSEWSPCTVTCGKGLKVRTRLYLKTSA 530          

HSP 2 Score: 72.4034 bits (176), Expect = 2.158e-12
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 0

Query:   28 LLVVDPQE--DDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            +L+V+P+E  +D SS  D             GSFQL GD + ++S+ C N +   S + K +IQV+W APP+G+GCV FK TV E R
Sbjct:    1 MLIVEPKEIKNDISSRPDV------------GSFQLFGDAMCKYSEECPNAIVHASLIPKQDIQVMWKAPPAGTGCVVFKATVQENR 75          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: XP_006569018.1 (PREDICTED: spondin-1 [Apis mellifera])

HSP 1 Score: 333.954 bits (855), Expect = 3.081e-104
Identity = 169/390 (43.33%), Postives = 245/390 (62.82%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEG----SSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLN----NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMK 494
            FW     A+ G  Q+A+WGS   +E+EL+A S+ +RT+IKA GLWYPN    T   FR D+K  ++S+ SM GPSPDW+VGVS L LCRK+C+W + + + LYPWDAG D+GISY SP+S T P+  +  I++ +P DPR PFYD +G  M PLA+L L R+++  + C DE+ L        +  V++ E     ED   P CQ +E++ WS+CSV+CGKGL MRTR +L+P KA+M  C+RQLV KEMC S +P+C G      G  + A G+   D  C T++W  WSECS++CG G + RTRRF +RMGRK+CPH+  +EK+ C        L+ E+ D  CK  +    +  SA     + ++ + +MV+  +++    + E+++
Sbjct:  225 FWGKDHVATDGFRQLAEWGSASGVETELRANSNKLRTLIKAAGLWYPNVNSNTTTNFRVDRKRPMISVASMFGPSPDWVVGVSKLNLCRKDCSWTKSEIIDLYPWDAGTDNGISYMSPNSETTPREKMKPITTLYPEDPRSPFYDPSGRPMLPLARLYLTREKIIPRGC-DEEVL--------QQQVAQLEVAENTEDTVRPECQTTEYTGWSSCSVSCGKGLRMRTRSYLMPEKAAMFKCNRQLVSKEMCVSSIPECSGEEDTDDGVGSLAGGNN--DAFCETTDWGHWSECSSSCGVGIKMRTRRFKDRMGRKRCPHVYLVEKVKCMEPACSPGLE-EQIDPACKVTDWSDWSPCSASCGKGVKLRTRLLMVDPSKQQECSSRMELVQ 602          

HSP 2 Score: 75.0998 bits (183), Expect = 3.208e-13
Identity = 49/150 (32.67%), Postives = 73/150 (48.67%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEE--RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLW 157
            I L G +T    ++F  F L V+ Q          H P   + + R G FQL  D LT F++ C NT+++ S   K+E+QV+W APPSGSGCV F   V+E   R++   G  +K   ++        L+ E     D  +  +   G+W
Sbjct:   57 ISLQGSQTHERLQQFTHFTLSVNSQ----------HAP--SNPTARVGYFQLFPDGLTTFNEDCVNTISEASDYPKNEVQVMWRAPPSGSGCVIFTAMVLENNVRWYAEDGALTKTFCEMDS-SEVEALDGEKCCACDEAKYSLIMEGIW 193          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: XP_006569017.1 (PREDICTED: spondin-1 [Apis mellifera])

HSP 1 Score: 333.954 bits (855), Expect = 3.081e-104
Identity = 169/390 (43.33%), Postives = 245/390 (62.82%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEG----SSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLN----NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMK 494
            FW     A+ G  Q+A+WGS   +E+EL+A S+ +RT+IKA GLWYPN    T   FR D+K  ++S+ SM GPSPDW+VGVS L LCRK+C+W + + + LYPWDAG D+GISY SP+S T P+  +  I++ +P DPR PFYD +G  M PLA+L L R+++  + C DE+ L        +  V++ E     ED   P CQ +E++ WS+CSV+CGKGL MRTR +L+P KA+M  C+RQLV KEMC S +P+C G      G  + A G+   D  C T++W  WSECS++CG G + RTRRF +RMGRK+CPH+  +EK+ C        L+ E+ D  CK  +    +  SA     + ++ + +MV+  +++    + E+++
Sbjct:  225 FWGKDHVATDGFRQLAEWGSASGVETELRANSNKLRTLIKAAGLWYPNVNSNTTTNFRVDRKRPMISVASMFGPSPDWVVGVSKLNLCRKDCSWTKSEIIDLYPWDAGTDNGISYMSPNSETTPREKMKPITTLYPEDPRSPFYDPSGRPMLPLARLYLTREKIIPRGC-DEEVL--------QQQVAQLEVAENTEDTVRPECQTTEYTGWSSCSVSCGKGLRMRTRSYLMPEKAAMFKCNRQLVSKEMCVSSIPECSGEEDTDDGVGSLAGGNN--DAFCETTDWGHWSECSSSCGVGIKMRTRRFKDRMGRKRCPHVYLVEKVKCMEPACSPGLE-EQIDPACKVTDWSDWSPCSASCGKGVKLRTRLLMVDPSKQQECSSRMELVQ 602          

HSP 2 Score: 75.0998 bits (183), Expect = 3.208e-13
Identity = 49/150 (32.67%), Postives = 73/150 (48.67%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEE--RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLW 157
            I L G +T    ++F  F L V+ Q          H P   + + R G FQL  D LT F++ C NT+++ S   K+E+QV+W APPSGSGCV F   V+E   R++   G  +K   ++        L+ E     D  +  +   G+W
Sbjct:   57 ISLQGSQTHERLQQFTHFTLSVNSQ----------HAP--SNPTARVGYFQLFPDGLTTFNEDCVNTISEASDYPKNEVQVMWRAPPSGSGCVIFTAMVLENNVRWYAEDGALTKTFCEMDS-SEVEALDGEKCCACDEAKYSLIMEGIW 193          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: XP_003251296.1 (PREDICTED: spondin-1 [Apis mellifera])

HSP 1 Score: 333.954 bits (855), Expect = 3.081e-104
Identity = 169/390 (43.33%), Postives = 245/390 (62.82%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEG----SSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLN----NSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMK 494
            FW     A+ G  Q+A+WGS   +E+EL+A S+ +RT+IKA GLWYPN    T   FR D+K  ++S+ SM GPSPDW+VGVS L LCRK+C+W + + + LYPWDAG D+GISY SP+S T P+  +  I++ +P DPR PFYD +G  M PLA+L L R+++  + C DE+ L        +  V++ E     ED   P CQ +E++ WS+CSV+CGKGL MRTR +L+P KA+M  C+RQLV KEMC S +P+C G      G  + A G+   D  C T++W  WSECS++CG G + RTRRF +RMGRK+CPH+  +EK+ C        L+ E+ D  CK  +    +  SA     + ++ + +MV+  +++    + E+++
Sbjct:  225 FWGKDHVATDGFRQLAEWGSASGVETELRANSNKLRTLIKAAGLWYPNVNSNTTTNFRVDRKRPMISVASMFGPSPDWVVGVSKLNLCRKDCSWTKSEIIDLYPWDAGTDNGISYMSPNSETTPREKMKPITTLYPEDPRSPFYDPSGRPMLPLARLYLTREKIIPRGC-DEEVL--------QQQVAQLEVAENTEDTVRPECQTTEYTGWSSCSVSCGKGLRMRTRSYLMPEKAAMFKCNRQLVSKEMCVSSIPECSGEEDTDDGVGSLAGGNN--DAFCETTDWGHWSECSSSCGVGIKMRTRRFKDRMGRKRCPHVYLVEKVKCMEPACSPGLE-EQIDPACKVTDWSDWSPCSASCGKGVKLRTRLLMVDPSKQQECSSRMELVQ 602          

HSP 2 Score: 75.0998 bits (183), Expect = 3.208e-13
Identity = 49/150 (32.67%), Postives = 73/150 (48.67%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEE--RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLW 157
            I L G +T    ++F  F L V+ Q          H P   + + R G FQL  D LT F++ C NT+++ S   K+E+QV+W APPSGSGCV F   V+E   R++   G  +K   ++        L+ E     D  +  +   G+W
Sbjct:   57 ISLQGSQTHERLQQFTHFTLSVNSQ----------HAP--SNPTARVGYFQLFPDGLTTFNEDCVNTISEASDYPKNEVQVMWRAPPSGSGCVIFTAMVLENNVRWYAEDGALTKTFCEMDS-SEVEALDGEKCCACDEAKYSLIMEGIW 193          

BLAST of EMLSAG00000011205 vs. Select Arthropod Genomes
Match: XP_016769226.1 (PREDICTED: spondin-1-like isoform X3 [Apis mellifera])

HSP 1 Score: 335.88 bits (860), Expect = 1.608e-103
Identity = 185/443 (41.76%), Postives = 251/443 (56.66%), Query Frame = 0

Query:  108 VVEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTI 550
             V+ RFWEY G AS GL+QVA++G  R LESELK +SDHIRTIIKARG+ YPN  GKTFAVFR D+K+HL+SLVSM+ PSPDWIVGVSGLELC+ NC+W+E KEL LYP+DAG D+GI+Y SPDSPTEP+  I RI+S +PND R PFYD +G+ MKPLA+L LNRQRLY+K+C +   +P D +M+  +   K  ++          C+V+ W+ W  CSVTCG+G  +R R +     A+   C+  L  +  C  E+P C              L  + CA  +W+ WS CS TCG   ++R+R F  +   K+C  I                            L  + +  +    DM K ++            Q E  K      + ++D + E   M+N       +   T WS WS C+++CG G + +FR +
Sbjct:  235 TVDYRFWEYNGMASTGLQQVAEFGLTRKLESELKNQSDHIRTIIKARGISYPNVTGKTFAVFRVDQKHHLMSLVSMIDPSPDWIVGVSGLELCQSNCSWIEHKELNLYPYDAGTDNGITYLSPDSPTEPQEAIHRITSSYPNDSRSPFYDPSGLDMKPLARLYLNRQRLYEKTCEE---IPGD-AMNTGTPDKKRRDK---------ACKVTSWAPWEACSVTCGRGTRLRQRFYEDKAAATQNKCNVTLTSRAKCQGEIPHCNNRG-----RDAGILETEKCALHDWSTWSSCSATCGDSHKTRSRNFKYKKHLKECKSIPNG-----------------------PVLQETIACEYVQCPDMDKDEVSESPS-------QEEENKNNEQDNDNEEDYEGEAPVMENC-----PEERYTQWSLWSPCSSTCGPGIQLRFRLV 624          

HSP 2 Score: 68.9366 bits (167), Expect = 3.310e-11
Identity = 35/101 (34.65%), Postives = 52/101 (51.49%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVE 110
            + +  D+   I RKF  FL+              S  P S + +   G F L  D+LT++S++C NTV + S V K EI V W +P  GSGC+  + T++E
Sbjct:   72 VTVQADRMGIIPRKFTRFLI--------------SSEPESEEDTAESGIFDLQDDLLTKYSENCPNTVVEMSMVSKEEISVAWTSPSEGSGCIFIRATILE 158          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|1022758457|gb|KZS04951.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 380.178 bits (975), Expect = 2.435e-120
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  179 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 493          

HSP 2 Score: 80.4925 bits (197), Expect = 2.946e-12
Identity = 47/112 (41.96%), Postives = 64/112 (57.14%), Query Frame = 0

Query:    1 MVIYEFSLHIRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            + +   ++   +  +K+  + +KF  FLL   P     +SI     P S D +G   SF+L  D L+RFSD C N +TQTSSV K+EIQVLW AP  G+GC+ F   VVE R
Sbjct:    3 IALKSAAIPTNISLEKSSNVLQKFVGFLLTATPSI---TSI-----PTSPDTAG---SFRLFSDSLSRFSDVCPNGITQTSSVPKTEIQVLWTAPGPGTGCIKFSAVVVENR 103          

HSP 3 Score: 66.6254 bits (161), Expect = 6.348e-8
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  343 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 534          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942353901|gb|JAN58880.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.867 bits (969), Expect = 1.097e-119
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  104 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 418          

HSP 2 Score: 65.0846 bits (157), Expect = 2.244e-7
Identity = 59/209 (28.23%), Postives = 92/209 (44.02%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSD---CSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM     M +N + +          D G   S+   C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  268 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEM----CMGMNFECE----------DSGFDESEDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 459          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|321466767|gb|EFX77761.1| (hypothetical protein DAPPUDRAFT_321105 [Daphnia pulex])

HSP 1 Score: 382.104 bits (980), Expect = 1.247e-119
Identity = 197/335 (58.81%), Postives = 241/335 (71.94%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD AG  MKPLA++++ RQR+Y+K+C+D   + ED        VS  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  235 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPAGSDMKPLARVVITRQRVYEKACTDTSTITED-------AVSHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDICAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMKFEC 549          

HSP 2 Score: 83.9593 bits (206), Expect = 2.761e-13
Identity = 46/103 (44.66%), Postives = 61/103 (59.22%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            + L G ++Q + +KF  FLL   P         +   P S D +G   SF+L  D L+RFSD C+N +TQTSSV K+EIQVLW AP  G+GC+ F   V+E R
Sbjct:   68 VTLQGWRSQNVLQKFIGFLLTATPS--------NPAIPTSPDTAG---SFRLFSDSLSRFSDVCSNGITQTSSVPKTEIQVLWTAPGPGTGCIKFSAVVLENR 159          

HSP 3 Score: 68.9366 bits (167), Expect = 1.432e-8
Identity = 60/206 (29.13%), Postives = 91/206 (44.17%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D IC         + S + G  F    ++    + +++  L   + EM             M+ E ++   DE     DC V+ WS WS C+A+CGKG + + R  LV
Sbjct:  399 RQRVYEKACTDTSTITEDAVSHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDICAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMKFECEDSGFDES-DDPDCLVSPWSDWSPCSATCGKGMQVRSRIPLV 590          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942356591|gb|JAN60225.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.867 bits (969), Expect = 1.275e-119
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  104 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 418          

HSP 2 Score: 65.0846 bits (157), Expect = 2.040e-7
Identity = 59/209 (28.23%), Postives = 92/209 (44.02%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSD---CSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM     M +N + +          D G   S+   C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  268 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEM----CMGMNFECE----------DSGFDESEDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 459          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942353903|gb|JAN58881.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.481 bits (968), Expect = 3.004e-119
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  124 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 438          

HSP 2 Score: 65.4698 bits (158), Expect = 1.604e-7
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  288 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 479          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942338903|gb|JAN51384.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.096 bits (967), Expect = 3.234e-119
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  117 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 431          

HSP 2 Score: 65.4698 bits (158), Expect = 1.621e-7
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  281 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 472          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942327905|gb|JAN45885.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.096 bits (967), Expect = 4.006e-119
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  117 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 431          

HSP 2 Score: 65.4698 bits (158), Expect = 1.799e-7
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  281 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 472          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942367377|gb|JAN65618.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 377.096 bits (967), Expect = 1.988e-118
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  187 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 501          

HSP 2 Score: 65.855 bits (159), Expect = 1.337e-7
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  351 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 542          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942367379|gb|JAN65619.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 378.252 bits (970), Expect = 2.407e-118
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  236 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 550          

HSP 2 Score: 80.1073 bits (196), Expect = 4.892e-12
Identity = 47/103 (45.63%), Postives = 60/103 (58.25%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            + L   ++  + +KF  FLL   P     +SI     P S D +G   SF+L  D L+RFSD C N +TQTSSV K+EIQVLW AP  G+GC+ F   VVE R
Sbjct:   69 VTLQNLRSSNVLQKFVGFLLTATPSI---TSI-----PTSPDTAG---SFRLFSDSLSRFSDVCPNGITQTSSVPKTEIQVLWTAPGPGTGCIKFSAVVVENR 160          

HSP 3 Score: 66.2402 bits (160), Expect = 8.942e-8
Identity = 58/206 (28.16%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ WS WS C+A+CGKG + + R  LV
Sbjct:  400 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWSDWSPCSATCGKGMQVRSRIPLV 591          

BLAST of EMLSAG00000011205 vs. nr
Match: gi|942294507|gb|JAN29186.1| (Spondin-1 [Daphnia magna])

HSP 1 Score: 378.252 bits (970), Expect = 3.051e-118
Identity = 195/335 (58.21%), Postives = 240/335 (71.64%), Query Frame = 0

Query:  109 VEERFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
             + R WEYGG AS GL QVA+WGS + LESELK+ESDHIRTIIK+RGLWYPN  GKT+AVFR D+K+HL+SLVSM+GPSPDWIVGVS LELC +NCTWVE K L LYPWDAG DSGI+Y S +S T P+  ITRI+S +P DPR PFYD  G  MKPLA++++ RQR+Y+K+C+D   + ED        +S  +  G   D   P C V+EW  +S CSVTCGKGL MR R ++   KA M  CDRQL +KEMC+S V  C           G  + DD+CA SEW++WS CS TCGKGFR+RTRR+++RMGRKKC H++T E+ MC G   +C
Sbjct:  236 ADYRVWEYGGIASDGLRQVAEWGSTQALESELKSESDHIRTIIKSRGLWYPNVNGKTYAVFRVDRKHHLMSLVSMLGPSPDWIVGVSALELCSRNCTWVENKTLNLYPWDAGTDSGITYTSTNSMTNPREKITRITSSYPADPRSPFYDPTGSDMKPLARVVITRQRVYEKACTDTSTITED-------AISHPDNSG---DALRPECAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDRQLEEKEMCASAVELC---------GDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKC-HLETSEQEMCMGMNFEC 550          

HSP 2 Score: 80.1073 bits (196), Expect = 4.833e-12
Identity = 47/103 (45.63%), Postives = 60/103 (58.25%), Query Frame = 0

Query:   10 IRLDGDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEER 112
            + L   ++  + +KF  FLL   P     +SI     P S D +G   SF+L  D L+RFSD C N +TQTSSV K+EIQVLW AP  G+GC+ F   VVE R
Sbjct:   69 VTLQNLRSSNVLQKFVGFLLTATPSI---TSI-----PTSPDTAG---SFRLFSDSLSRFSDVCPNGITQTSSVPKTEIQVLWTAPGPGTGCIKFSAVVVENR 160          

HSP 3 Score: 64.3142 bits (155), Expect = 3.991e-7
Identity = 57/206 (27.67%), Postives = 89/206 (43.20%), Query Frame = 0

Query:  355 RQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRK--KCPHIDTIEKLMCAGNTADCSLDNEECDRICKSL------NNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILV 552
            RQ V ++ C+      E +     N+     P+  CA +EW ++S CS TCGKG R R R + N+M  +  +C      EK MCA     C   +   D +C         + S + G  F    ++    + +++  L   + EM             M  E ++   DE     +C V+ W  WS C+A+CGKG + + R  LV
Sbjct:  400 RQRVYEKACTDTSTITEDAISHPDNSGDALRPE--CAVTEWGQFSPCSVTCGKGLRMRQRFYINQMKAEMTRCDR-QLEEKEMCASAVELCGDGSMVKDDVCAVSEWSQWSSCSVTCGKGFRTRTRRYYDRMGRKKCHLETSEQEMCM----------GMNFECEDSGFDES-EDPECLVSDWRDWSPCSATCGKGMQVRSRIPLV 591          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold94_size379870-snap-gene-0.14 (protein:Tk09624 transcript:maker-scaffold94_size379870-snap-gene-0.14-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_321105")

HSP 1 Score: 522.316 bits (1344), Expect = 2.983e-176
Identity = 247/332 (74.40%), Postives = 285/332 (85.84%), Query Frame = 0

Query:  112 RFWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHEGKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADC 443
            R W+Y GY+SKGL QVAKWGSPR+LESELKAES HIRTIIKARGLWYPN  GKTFAVFRTD+KNHLVSLVSM+GPSPDWIVGVSGLELC KNCTW +++EL+LYPWDAGVDSGISYESPDS T P+ PI RI+SR P+DP QPFY+ +G  MKPLAKL + RQRLYKKSC + D+    E + F S V+  E   V ED S+PGC VS+W++WS+CSVTCGKG+SMRTR FL+P KA+M+GCDRQ+VQKEMC+SEVP CEG+  FY +AP  FLPDDMCATSEW++WSECSTTCGKGF+SRTRRFFNRMGRKKCPH+DT+EK MCAG+TA C
Sbjct:  183 RVWQYNGYSSKGLSQVAKWGSPRVLESELKAESQHIRTIIKARGLWYPNVNGKTFAVFRTDRKNHLVSLVSMLGPSPDWIVGVSGLELCLKNCTWAKKRELYLYPWDAGVDSGISYESPDSLTFPQQPIKRITSRDPDDPHQPFYNASGAPMKPLAKLTVTRQRLYKKSCGN-DYEDRSEDLMF-SDVN--EHDSVFEDHSLPGCDVSQWTEWSSCSVTCGKGISMRTRTFLMPEKAAMLGCDRQMVQKEMCASEVPICEGNR-FYLSAPEGFLPDDMCATSEWSRWSECSTTCGKGFKSRTRRFFNRMGRKKCPHVDTMEKSMCAGDTAVC 509          

HSP 2 Score: 124.405 bits (311), Expect = 6.842e-30
Identity = 62/96 (64.58%), Postives = 71/96 (73.96%), Query Frame = 0

Query:   14 GDKTQFIERKFREFLLVVDPQEDDGSSIFDSHRPPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVV 109
            GDKT+ +ER+F+EFLLVV+    +     D H       +G  GS+QLLGDVLTRFSDHCTNTVTQTS V KSEIQVLW APP+GSGCV FK TVV
Sbjct:   10 GDKTKVMERRFKEFLLVVE----NSDEWPDQHSSAVKQPAGSSGSYQLLGDVLTRFSDHCTNTVTQTSDVEKSEIQVLWTAPPAGSGCVTFKATVV 101          

HSP 3 Score: 80.1073 bits (196), Expect = 8.546e-16
Identity = 59/196 (30.10%), Postives = 88/196 (44.90%), Query Frame = 0

Query:  267 AKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDR-QLVQKEMCSSEVPKC----EGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFF---NRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRIC 454
            A L  +RQ + K+ C+ E  + E    +  +       +G   D+    C  SEWS WS CS TCGKG   RTR F    +     C     ++K MC+ +   C    +G  G       D   D  C  + W++WS CS +CGKG + RTR +     +     C ++  +EK  C G+ A+C  + +    IC
Sbjct:  416 AMLGCDRQMVQKEMCASEVPICEGNRFYLSA------PEGFLPDDM---CATSEWSRWSECSTTCGKGFKSRTRRFF--NRMGRKKCPHVDTMEKSMCAGDTAVCPQYPDGGDG-----STDPENDPHCGVTSWSEWSPCSVSCGKGLKVRTRLYKVSPQQQTNADC-NVQLMEKASCDGSRAECEYNPQNAKMIC 594          

HSP 4 Score: 77.0258 bits (188), Expect = 7.241e-15
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = 0

Query:  489 QHEMMKTEIMMLNKKKDMQKEM----KNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKMSKKTGKEAKMRLTS 575
            Q+EM K + M + K+KD+  ++    K   N++ +   DC VT+WSPWS CN+SCGK FRRKFRTI    + +G+   KK  ++ K RL S
Sbjct:  771 QNEMEKMKQMEMAKQKDIMNQLERARKAADNEDELRVVDCLVTAWSPWSDCNSSCGKAFRRKFRTIQRYPTDNGRSCPKKLERKQKCRLPS 861          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold327_size205035-snap-gene-1.9 (protein:Tk05413 transcript:maker-scaffold327_size205035-snap-gene-1.9-mRNA-1 annotation:"PREDICTED: spondin-1-like")

HSP 1 Score: 121.709 bits (304), Expect = 4.393e-29
Identity = 62/135 (45.93%), Postives = 80/135 (59.26%), Query Frame = 0

Query:  184 MGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSK---EEEQGVEEDESIPG 315
            M PSPDWIVG+S   LC  +CTWVE + + LYPWD G  SG+ Y+SPD PT P+ PI RI+S  PN+P  PF+D+    MKP A+L L +QR Y+K C      P +      SG+      E+   E +  IPG
Sbjct:    1 MIPSPDWIVGLSKENLCLDDCTWVENRVIDLYPWDVGTQSGLRYDSPDEPTVPRSPIQRITSSNPNNPASPFFDETSAPMKPAARLHLLKQREYRKPCPPGRMGPINNMNPSWSGIGGAFMPEQSYPESNMGIPG 135          

HSP 2 Score: 76.2554 bits (186), Expect = 1.183e-14
Identity = 51/138 (36.96%), Postives = 68/138 (49.28%), Query Frame = 0

Query:  316 CQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKC-----EGSSGFYANAPGDFLP--DDMCATSEWTKWSECSTTCGKGFRSRTRRF-FNRMGRKKCPHIDTIEKLMCAGNTADCSL 445
            CQV++W  WS CS  CG G   R R +    K+S  GC  +L  +E C  + P C      G+   + N  G F P  D MCA + W +WS CST CG G R RTR F    +  ++C  +   +K  C G    C+L
Sbjct:  388 CQVTQWGAWSDCSSLCGTGHRTRRRRYTEHDKSS--GCSEELDDQEPCVGQGPGCPNHNPSGTEYGFTNQQGFFNPMADMMCALTSWGEWSPCSTKCGSGTRMRTRLFKVPFVDNRRC-DVSLFKKESCFGMEESCNL 522          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold146_size311726-snap-gene-1.22 (protein:Tk07051 transcript:maker-scaffold146_size311726-snap-gene-1.22-mRNA-1 annotation:"GI18408")

HSP 1 Score: 74.3294 bits (181), Expect = 2.780e-14
Identity = 66/239 (27.62%), Postives = 112/239 (46.86%), Query Frame = 0

Query:   47 PPSMDASGRGGSFQLLGDVLTRFSDHCTNTVTQTSSVVKSEIQVLWMAPPSGSGCVNFKTTVVEERF--WEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWYPNHE---GKTFAVFRTDKKNHLVSLVSMMGPSPDWIVGVSGLELCRKNCTWVEEKELFLYPWDAGVDSGISYESPDSPTEPKLPITRISSRFPNDPRQPFYDDAGVLMKPLAKLILNRQRLYKKS 280
            PP + A+  G     L  V+ +F     NT   +S+  K   +  W  P   S  V F     E+ F  ++ G  +S G+++ A+ G    L+ ++  ES          G   P  E   G + A    D  + +VS++S + PSPDW VG+ GL LC +N  +++   +   P DAG D+G ++ SP+ PT P+  + RI++ +P  P   F+      +  +A     +++ Y+ S
Sbjct:   41 PPEVQATSEGCQSDKL--VVYKF---IFNTYWDSSTFPKQYPE--WRPPAQWSKVVGFSH---EDDFHLFQMGQESSLGVKEFAETGETETLD-KMAEES-------VLDGFHAPPIETGAGHSHAKVFIDGNHTMVSVISKIVPSPDWFVGLDGLNLC-QNGRFIDSVTVEADPMDAGTDNGFTFTSPNWPTVPQAVVFRITNTYPTHPAGSFHYPQLASLPTIASFSFIKEKEYELS 260          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold327_size205035-snap-gene-1.8 (protein:Tk05414 transcript:maker-scaffold327_size205035-snap-gene-1.8-mRNA-1 annotation:"GH22804")

HSP 1 Score: 67.781 bits (164), Expect = 6.617e-14
Identity = 37/71 (52.11%), Postives = 48/71 (67.61%), Query Frame = 0

Query:  113 FWEYGGYASKGLEQVAKWGSPRILESELKAESDHIRTIIKARGLWY-PNHEGKTFAVFRTDKKNHLVSLVS 182
            FWEYG  A++ L+ + + G    LES++K  SD IR++IKA GL    N  G+TFAVFR D  +HLVSLVS
Sbjct:   53 FWEYGQTATQALQLLGEKGITFRLESQMKLFSDSIRSVIKAHGLQQRSNVMGRTFAVFRVDPNHHLVSLVS 123          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold591_size129331-processed-gene-0.4 (protein:Tk08230 transcript:snap_masked-scaffold591_size129331-processed-gene-0.4-mRNA-1 annotation:"SCO-spondin")

HSP 1 Score: 67.781 bits (164), Expect = 6.499e-12
Identity = 79/290 (27.24%), Postives = 118/290 (40.69%), Query Frame = 0

Query:  318 VSEWSDWSTCSVTCGKGLSMRTRDFLIPGK---------ASMMGCDRQLVQKEMCS----------SEVPKCEGSSG----------FYANA----PGDFLPDDMCATS------EWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDR-ICKSLNNSTSAGHSFI-IDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTIL------VQSSSHGKK 560
            + EW++WS CSV+CG G   RTR   +PG          AS   C + L + E C+          SE  +C  + G            AN+     G    D  C  S      EW +WSECS TCG G R  TR+        + P+ + ++   C    A    + E C+   C  L   T  G   +     +K  M E    +L+   +     ++ +L K +   +E               ++T W+ WSQC+ +CG GFR+K R  +      V SSS+G +
Sbjct:  772 LGEWTEWSQCSVSCGGGDRSRTRTCGVPGSTQIASASTPASENPCQQPLFESEKCNDDRCPVYTPWSEWSQCSATCGGGSQRRNRQCVPANSRLFCEGVSEEDRTCNESPCPNWTEWGEWSECSFTCGGGTRQNTRQC-------ELPNGEQVKGSQCGVGPA---TEEESCNTDQCPELGPWTEWGPCTVTCGGGEKTRMRECGLPKLLRDDN---PCQLPLLEKMECHPEECP-------------TLTEWADWSQCSHTCGGGFRQKTRECVGPNANDVSSSSNGDE 1035          

HSP 2 Score: 60.077 bits (144), Expect = 1.540e-9
Identity = 63/275 (22.91%), Postives = 103/275 (37.45%), Query Frame = 0

Query:  284 EDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGK-----ASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSV-TSWSPWSQCNASCGKGFRRKFRTILV 552
            E  +P D   +  +G ++ +E   E     P    SEW+DW  C+ TCG G   R RD L+         +  GCD    +   C+              N P           +EWT++++CS TCG G R +TR   N       P +     L C G+           +++ +  N  T           K     E     +        KT   +  + +D     +N+ ++E  +   C   T W+ W+ C  +C KG R++ R  ++
Sbjct: 1132 ECIVPRDGDANICNGETRTQESCNE----TPCPVWSEWTDWGQCTATCGGGTEKRIRDCLLAAARNGNGTNFYGCDGDTWEMRGCNEH------------NCP---------TWTEWTEFTDCSKTCGGGRRVKTRE-CNLPPNIPAPAL----ALFCPGD-----------EKVIEDCNTGT---------CPKPTEWTEWGECSVSCGGGTRQKTRECVNFRDQDGNPCNENLVDEEPCNEQPCPAWTEWTEWTSCTKTCDKGRRKRARDCVL 1356          

HSP 3 Score: 59.6918 bits (143), Expect = 2.224e-9
Identity = 58/252 (23.02%), Postives = 92/252 (36.51%), Query Frame = 0

Query:  318 VSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMM--------GCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCAT-SEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSVTS-WSPWSQCNASCGKGFRRKFRTILVQSSSHGK 559
            ++EW+DWS CS TCG G   +TR+ + P    +          C   L   E C+                      +D C   S W +W+ CS TCG G R+R R         +C    T ++  C G+      ++E+C+                  D        E     L        K    ++ +  D        +  E  + + C V S W+ W QC A+CG G  ++ R  L+ ++ +G 
Sbjct:  995 LTEWADWSQCSHTCGGGFRQKTRECVGPNANDVSSSSNGDENACMEPLEVIEACN----------------------EDTCPVWSVWGEWTICSKTCGGGERTRYR---------QCTDPVTGQEAFCPGSNE----ESEDCN----------------TQDCPYFTEWTEWTDCSLTCGSGTRSKVRECIVPRDGDANICNGETRTQESCNETPCPVWSEWTDWGQCTATCGGGTEKRIRDCLLAAARNGN 1195          

HSP 4 Score: 55.0694 bits (131), Expect = 6.489e-8
Identity = 67/293 (22.87%), Postives = 101/293 (34.47%), Query Frame = 0

Query:  266 LAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCAT-SEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDCSV------TSWSPWSQCNASCGKGFRRKFRTIL 551
             +K     +R   + C+D    P      F  G ++E E    +D        +EW++W+ CS+TCG G   + R+ ++P       C+ +   +E C +E P                     C   SEWT W +C+ TCG G   R R       R      +      C G+T +         R C   N  T    +   D  K                   +KT    L               DE +   DC+       T W+ W +C+ SCG G R+K R  +
Sbjct: 1065 CSKTCGGGERTRYRQCTD----PVTGQEAFCPGSNEESEDCNTQDCPY----FTEWTEWTDCSLTCGSGTRSKVRECIVPRDGDANICNGETRTQESC-NETP---------------------CPVWSEWTDWGQCTATCGGGTEKRIRDCLLAAARNG----NGTNFYGCDGDTWE--------MRGCNEHNCPTWTEWTEFTDCSK------------TCGGGRRVKTRECNLPPNIPAPALALFCPGDEKVIE-DCNTGTCPKPTEWTEWGECSVSCGGGTRQKTRECV 1302          

HSP 5 Score: 54.299 bits (129), Expect = 9.208e-8
Identity = 34/97 (35.05%), Postives = 48/97 (49.48%), Query Frame = 0

Query:  319 SEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCAT-SEWTKWSECSTTCGKGFRSRTR 414
            ++WS WS CS +CG G + R+R+  +P  A                   P  E   G  +N  G+   + +C    EWT+WS+CS +CG G RSRTR
Sbjct:  716 TQWSAWSQCSASCGGGQNARSRECTLPDGAVR-----------------PNAECGEG-ESNETGNCNENVLCPVLGEWTEWSQCSVSCGGGDRSRTR 794          

HSP 6 Score: 51.9878 bits (123), Expect = 5.339e-7
Identity = 70/333 (21.02%), Postives = 118/333 (35.44%), Query Frame = 0

Query:  266 LAKLILNRQRLYKKSCSDEDFLPEDESMHFESGVSKEEEQGVEEDESIPGC-QVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKASMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRR--FFNR------------MGRKKCPHID-----TIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKKDMQKEMKNMQNDEGISRSDC-----------------SVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHGKKM 561
             +K     +R+  + C+    +P      F  G  K     V ED +   C + +EW++W  CSV+CG G   +TR+ +         C+  LV +E C+ +                        A +EWT+W+ C+ TC KG R R R    F R               + CP +      T   L C G T           R+   L+  +    +  ++  ++ M+          +  E  +         K   +E    QN E  ++  C                   T W+ W  C+ +CG+G R++ R       ++GK +
Sbjct: 1229 CSKTCGGGRRVKTRECNLPPNIPAPALALFCPGDEK-----VIEDCNTGTCPKPTEWTEWGECSVSCGGGTRQKTRECVNFRDQDGNPCNENLVDEEPCNEQPCP---------------------AWTEWTEWTSCTKTCDKGRRKRARDCVLFTRSDCPGEDEELEDCNPEPCPALTPWGEWTQCTLTCGGGTQ---------RRVRDCLSPISGFDSNGCMEPLEEIMVCSADACPAFTEWSEWTECSADCGGGTKTHVREC--TQNGEEAAQDTCLGDALEQVPCNDDVECPRWTEWNTWGDCSVTCGEGTRQRGRECTRPVETNGKLL 1524          

BLAST of EMLSAG00000011205 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold770_size100439-snap-gene-0.21 (protein:Tk05384 transcript:maker-scaffold770_size100439-snap-gene-0.21-mRNA-1 annotation:"Semaphorin-5B")

HSP 1 Score: 53.5286 bits (127), Expect = 1.724e-7
Identity = 59/263 (22.43%), Postives = 106/263 (40.30%), Query Frame = 0

Query:  305 QGVEEDESIPGCQVSEWSDWSTCSVTCGKGLSMRTRDFLIPGKA----SMMGCDRQLVQKEMCSSEVPKCEGSSGFYANAPGDFLPDDMCATSEWTKWSECSTTCGKGFRSRTRRFFNRMGRKKCPHIDTIEKLMCAGNTADCSLDNEECDRICKSLNNSTSAGHSFIIDMKKKQMMVEQQRKELVMKQHEMMKTEIMMLNKKK-----DMQKEMKNMQNDEGISRSDCSVTSWSPWSQCNASCGKGFRRKFRTILVQSSSHG 558
            Q +E        + ++WS WS+CS +C  G   R R    P  A    + +G D   +  + C+        +S   + +    L +     + W++WS CS  CG+GFRSR R+ F       C      ++      + +CS   E  D       N +  G S+     +K+     Q    + +  E+ + E    + ++       Q  M+N            ++ +W+ WS+C+  CGKG++ + R     S ++G
Sbjct:  618 QALEVTNCTQNGEWTDWSQWSSCSNSCDLGTQQRRRICGNPAPAYGGRTCIGPD---LDTQYCNDLPACASLASAAASVSSMRELEEPTSRWTPWSEWSPCSAHCGRGFRSRQRKCFGLSCTGSC------DREFVECESQECSDLLEVTDWTPWIRANHSGQGGSWY----EKRFRFNYQAPLAINQVGEISEEERFCTSTQQCSLISITQPTMRN------------NLPAWTEWSKCSRECGKGYQVRLRQCTPGSDAYG 855