hypothetical protein DAPPUDRAFT_333049, maker-scaffold1088_size63410-snap-gene-0.21 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000010843 (supercontig:LSalAtl2s:LSalAtl2s721:13130:108134:1 gene:EMLSAG00000010843 transcript:EMLSAT00000010843 description:"maker-LSalAtl2s721-augustus-gene-1.27") HSP 1 Score: 785.023 bits (2026), Expect = 0.000e+0 Identity = 455/1034 (44.00%), Postives = 627/1034 (60.64%), Query Frame = 0
Query: 222 YWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF-----NFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQT-GLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEK-----EDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKA-EDAAAAAARA----------------LTAEEEEELANQAP-----PVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF--AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGST--CDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKG---RLTVGKIYGGLLILENWKATK 1215
YW L+NL+ SL++S+ +I+SLL+LLFLF+ IFALLG Q+FGG + + + +NF+SFS ++L+VFQILTGEDWN VMY GI++ GG + G++ SLYFI L + GNY LLNVFLAIAVD+L A + EKE+ ED + I A G T+ R+ ++ + ED A L + + +++P P+ S+ +IF N FR H + F +++ I+ SS LAAEDP+N++S RNK L YFDY FT +F IEV LK+I YG +LHPG++ R +N++D+LVV+ ++ SF S+G +S +K LRVLRVLRPL+ I R LK V CVI + K + NI++V LL F+FAVI VQLF GKFF C+D SK A+ C+G++ +Y D + P + R+W R FHYDNV +LTLF V T EGWP +L S+ + D PI +R +++FY +Y I+ FF VNIFV +I+TFQ +GES + E+DKNQ++CI+FA+NA P+ RY+P+ + +Y+LW V S FEY I +I++NT L MK+YQQP + L +N +FT FT+E FK+ A K +YF D WN FDF+ V+GS++D M E N I++ F RLFR RLVKLL +G IR LLWTF +SF+ALP+V +LI ++FFIY ++GMQVFG I + NR+ NFQTF ++VLFR ATGEAW IML+C CD + G CG+ F+Y YF+SF +CSFL+LNLFVAVIMDNFDYLTRD SILG HHLDEF+R+W+EYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V+F TLF+L+R +LSIK +D+A+ ELR I+K+W + +D + P G G +TVGK Y LI + ++ K
Sbjct: 784 YWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAFGGVNSIGIVSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEAEKEEGAGPPEDGGVEGVDILGANSHC--GGDTELRLSLDQEYSLEDYDQYAEDDEIVGDDDDEEDEGQFELDDKNTLRMDSESPFYKVVPIPQESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIF----SSGA--ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCSDKSKMTAETCRGKFIVYKDGDLNQPIVEERIWERNDFHYDNVGKGLLTLFVVSTFEGWPGILYVSIDSNAEDVGPIHNYRPIVAVFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPI--QYKLWAFVTSPVFEYTIFAMILINTLSLAMKFYQQPQTYTAFLDVLNVIFTVFFTLEFVFKLGAFRFK-------------------------------------NYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGSNINRNNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGG--------CGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKRLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEG--NIDEANEELRHQILKIWKRTDLKLLDQICP-----GAGLEDEVTVGKFYATFLIQDYFRRFK 1755 HSP 2 Score: 197.593 bits (501), Expect = 3.227e-51 Identity = 120/298 (40.27%), Postives = 164/298 (55.03%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEV----------ILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQ 289
++ + GY WI +AE++ +L R E + + R GK + + + E KKK AL+K ++AR RK VK +W +I+LV LNTC +A EHY QP WL +F T F+ LF FEMFL+M++LG + YF S FNRFDC V+ S+ E++ T+ + GLSVLR +RLLR FKVT YWSS+ NLV SL++S+ SI SLL++LFLFI IF+LLGMQ+FGG F S+F++F + LTVFQ
Sbjct: 496 RQQIEEDLRGYLDWITQAEDLENDPSLPPPNLLGAKNKDSSTNRSSKEISRDSFSARLTNEADGKGGALDVDGTGEPSSWFQKKKK-----ALEK----------LNRRARRVCRKIVKSXAMFWIIIILVFLNTCVLATEHYKQPIWLDDFQEFTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTN-ENIMPPLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFT-KSLESRSHFDTFPQSCLTVFQ 776 HSP 3 Score: 101.293 bits (251), Expect = 1.069e-21 Identity = 70/284 (24.65%), Postives = 131/284 (46.13%), Query Frame = 0
Query: 84 LKKKFKKNSDFWVYEKKARFWIRKT---------VKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFG--GTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIE--------------SQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDS 342
L K + +F + K R +I K V F + + ++L+NT S+A++ Y QP + FL +F F E ++ A + YF +N FD +++ GS+ ++ P ++ R R++R+ K+ +R L+ + S +++ + +L+ L I+ ++GMQ+FG + +NF +F A+L +F+ TGE W E+M + + GG T Y YFI + ++ +LN+F+A+ +D+
Sbjct: 1318 LDKNQRNCIEFALNAKPVRRYIPKNPIQYKLWAFVTSPVFEYTIFAMILINTLSLAMKFYQQPQTYTAFLDVLNVIFTVFFTLEFVFKLGAFRFKNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGSNINRNNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYP-YFISFFVVCSFLILNLFVAVIMDN 1600 HSP 4 Score: 85.1149 bits (209), Expect = 9.514e-17 Identity = 76/335 (22.69%), Postives = 132/335 (39.40%), Query Frame = 0
Query: 410 LANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADD---CQGEYFLYSATDDHPPAIAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDK 733
+ + PP S++ ++ N R V + F+ I+L I+ + +ALA P + N+ L +Y F +F E ++KII YG HP +YLR WN++D +V + S + L G + +K+LR RVLRPL+ + VP+ E+ D+ C G Y D+ + W F ++D+ +MLT+F T EGW VL Q +M +++ I+++ ++ FF +N+ + ++ F ++ E + K
Sbjct: 139 IGAKPPPDRAPRSIYCWTIKNPIRKRFIQFVEWKPFEFLILLTILGNCVALAIYTPFPAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEGFD----VKALRAFRVLRPLRLVSGVPR-----------------------------------------------EMEETDNPTLCGGNY----KCDEEAGYVCREYWEGPNFGITNFDHFGLSMLTVFQCITLEGWTDVLYWVQDAMGSSW------------QFIYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQK 406 HSP 5 Score: 69.707 bits (169), Expect = 4.564e-12 Identity = 55/221 (24.89%), Postives = 99/221 (44.80%), Query Frame = 0
Query: 765 RIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMV-EFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFR 984
+IV+S + I+ L+ LNT +L ++Y+QP+ L D + NL F + F+ E LK+ S G YF +N FD V+ SI++ ++ E + + LR R R K+ R ++ L+ + V S ++ +++++ + FI++++GMQ+FG +F E H F TF + +F+
Sbjct: 596 KIVKSXAMFWIIIILVFLNTCVLATEHYKQPIW-------------------------------------LDDFQEFTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTNENIMPPLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGG-KFTKSLESRSH--FDTFPQSCLTVFQ 776 HSP 6 Score: 63.1586 bits (152), Expect = 4.607e-10 Identity = 53/236 (22.46%), Postives = 91/236 (38.56%), Query Frame = 0
Query: 836 TDILSYMNLTFTLCFSIECVLKLISYG----PTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRH----------VNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNF 1057
IL + F + F+ ECV+K+I+YG PT+Y + WN+ DF V+ ++ ++ +V LR FR R ++L+ R + T + GN + D + Y NF FG ++ +F+C T E W ++ + GS++ + YFVS + L +F ++NL + V+ F
Sbjct: 204 NQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEGFDVKALRAFRVLRPLRLVSG--VPREMEETDNPTLCG-----------------------GNYKCDEEAGYVCREYWEGPNFGITNFDHFGLSMLTVFQCITLEGWTDVLYWVQD-----------------------AMGSSWQFIYFVSXVVLGAFFVMNLILGVLSGEF 391 HSP 7 Score: 57.3806 bits (137), Expect = 2.573e-8 Identity = 43/185 (23.24%), Postives = 96/185 (51.89%), Query Frame = 0
Query: 90 KNSDFWVYEKKARFWI--RKTVKKQWFYWFVILLVLLNTCSVAVEH-YNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIY----FESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGL-SVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF 266
+ S F+++ +F I +F V++ +++++ +A E N ++ L+ ++ F +F E+ L++ + G ++ S FN D LV++ S+ +++ +G+ + +LR LR+LR + L+++V ++ + K+I +++L+ L +FA++G+QLF G F
Sbjct: 1012 QESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFS-----SGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKF 1191 HSP 8 Score: 54.6842 bits (130), Expect = 1.851e-7 Identity = 66/306 (21.57%), Postives = 118/306 (38.56%), Query Frame = 0
Query: 483 FTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRV---PKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRR-----------------------------------------------QKFHY-----DNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQE----QGESELEEA 729
F +F E+ LK+ G Y L+N D VV +I + EN+ L VLR +R L+T K + + ++NS+ ++ ++LV+ LF FIF+++ +Q+F GKF + Q ++ +D + R W+R K HY D+ ++L++F + T E W V+ + A F I + S+++I+ FI + +N+F+A+ + + + E+E EE
Sbjct: 640 FVVLFTFEMFLKMYSLGF----QGYFVSLFNRFDCFVVISSILELILT------NENIMPPLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFTKSLESRSHFDTFPQSCLTVFQGFED-----SERYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQA-FGGVNSIGIVS---SLYFIILFICGNYILLNVFLAIAVDNLADADALEDEAEKEEG 926
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000004952 (supercontig:LSalAtl2s:LSalAtl2s260:1045447:1062229:1 gene:EMLSAG00000004952 transcript:EMLSAT00000004952 description:"maker-LSalAtl2s260-snap-gene-10.24") HSP 1 Score: 758.829 bits (1958), Expect = 0.000e+0 Identity = 469/1139 (41.18%), Postives = 640/1139 (56.19%), Query Frame = 0
Query: 136 QPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRI------YFESSFNRFDCLVISGSVFEVVWT--HFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQT--GLIYSLYFIFLTLFGNYTLLNVFLAIAVDSL-GQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPG---PRTKERI---------------------------------EYERRKAEDAAAAAARALTAEEE-------EELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVD---ALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLAC--ESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPRE---EIGKGRLTVGKIYGGLLILENWK 1212
P L + LHT++ L I F R ++ YF S FNRFDC V+ S+ E+V T H P G+SVLR +RLLR FKVT YW+SL+NL+ SL++S+++I SL++LLFLF+ I+ALLG QLFGG F +ILTGEDWN VMY GI + GG QT G+ SLYFI L + GNY LLNVFLAIAVD+L G + K+ E A A + P PPG P +E + +Y + + + EE+ E+ N+ P+ P+SS+FIFS TN FR + F FI++ I+ SS +LAAEDP+++ SPRN +I YG + H G+Y R N++D++VVS +I S A SAG +S +K LRVLRVLRPL+ + R LK V VI S+ + NI++V +L F+FAVI VQLF GKF+ C+D SK ++CQG+Y + +D P + R W FHYDNV MLTLF V T EGWP +L S+ + D P+ + R M IFYIVY I+ FF VNIFV +I+TFQ++GES E+DKNQ++CID+ +NA P Y+P + V +YR+W S+ FEY + I+LNT L M +Y Q P + +D L +N FT+ F++E V+K+ ++ P YF D WN FD + V+GS +D + + I++ F RLFRA RLVKLL + +IR LLWTF++SF+ALPYV+LLIA++FFIY ++GMQVFG I + DT + + NFQ F S ++VLFR ATGEAW IML+C CD + + +G CGS +Y YF+SF SFL+LNLFVAVIMDNFDYLTRD SILGAHHLDEF+ +W+EYDP A+G I + ++ +L+ ++PPLGFG CP R A K+L+ MN P+ D+G V F TLF++IR +L IKM + ++ A+ ELR + K+W + +D L+P + E G + VGK Y +LI + ++
Sbjct: 365 DPLQLKQTLHTSKLAKLAK-INRKFRRACRKAVKSQVMFWNYFISLFNRFDCFVVLSSILELVLTRTHIMP---PLGMSVLRCIRLLRAFKVTRYWTSLKNLLKSLINSIEAIFSLIVLLFLFLGIYALLGSQLFGGKF--------------------XERILTGEDWNAVMYDGILAYGGPQTVLGMFASLYFITLFIIGNYILLNVFLAIAVDNLAGDDDDEEXETPVVKDPEPAFAPAQQDPSRPPPPGTQIPHEREDLLMMIDNHHLYNYDPGYEDEYNPYLNSNGYDHYNDYNQMVNNNGPIKSLEEKLEEEKANEEAFLEDDDNKKDPIPPFSSLFIFSPTNRFRVLCYNFCNHAYFANFILVCIMVSSASLAAEDPLHSKSPRNI--------------------VIAYGAVGHKGAYARYPANILDLVVVSVSIISIAFR--DSAGA--ISVLKILRVLRVLRPLRAVNRAKGLKLVIXAVIVSVSTIQNIVLVTVLLQFLFAVIGVQLFKGKFYSCSDSSKMTEEECQGQYVDFKKSDWAQPTVLEREWSLNDFHYDNVAKGMLTLFVVSTFEGWPGILYTSLDSHAEDTGPLPISRPSMFIFYIVYIIIIAFFMVNIFVGFVIVTFQKEGESPFANCELDKNQRNCIDYTLNAKPARLYLPSDPV--QYRIWAWAXSSKFEYVVFGAILLNTISLAMSFYNQ-------------------------------------PQIYSDFLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINEDTSIHSNNNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAG--------CGSIIAYPYFISFFIATSFLILNLFVAVIMDNFDYLTRDWSILGAHHLDEFVNLWSEYDPDAKGCIKHMDVVQLLRKINPPLGFGKLCPHRNACKRLVAMNMPLRDDGTVEFQATLFAIIRTSLGIKMEGS--LEXANSELRLIMKKIW-RKDDSVLDKLIPRKRGESEFGND-VAVGKFYATVLIQDCFR 1404 HSP 2 Score: 105.531 bits (262), Expect = 6.075e-23 Identity = 75/257 (29.18%), Postives = 130/257 (50.58%), Query Frame = 0
Query: 102 RFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEV-VWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPS--NFNSFSIAMLTVFQILTGEDWNEVMYHGIES----------QGGAQTG---LIYSLYFIFLTLFGNYTLLNVFLAIAVDS 342
R W K F + V +LLNT S+A+ YNQP S+FL ++F F E ++M A + YF +N FDC+++ GS ++ + + G ++ R R LR+ K+ S+R L+ + + S +++ ++LL+ L I+ ++GMQ+FG D + S NF +F A++ +F+ TGE W ++M I S + G G +I YFI + ++ +LN+F+A+ +D+
Sbjct: 995 RIWAWAXSSK--FEYVVFGAILLNTISLAMSFYNQPQIYSDFLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINEDTSIHSNNNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDN 1249 HSP 3 Score: 100.523 bits (249), Expect = 2.078e-21 Identity = 76/254 (29.92%), Postives = 127/254 (50.00%), Query Frame = 0
Query: 422 SMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPV-NTDSPR-NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDS------KEDADDCQGEYFLYSAT-DDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGW 666
S+++FS N R TVV + F+ FI+ I+A+ ++LA P N D+ N L + F +F E ++KI+ YG+ H +YLR WN +D +V MD+L S+ +K+LR RVLRPL+ + VP L+ V + ++ ++ +F+I ++ L I+++I +Q+F G D+ +D C G Y + ++ P P ++DN +MLT+F T EG+
Sbjct: 47 SIYLFSLKNPIRLKCITVVEWKAFEFFILATILATCVSLALHKPYPNGDTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGF----MDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMFQGLMHKACFDNITGVLIDDDPRLCGGNYQCETGVCREYIP--GPNFGIT---NFDNFILSMLTVFQCITLEGF 291 HSP 4 Score: 63.929 bits (154), Expect = 2.560e-10 Identity = 35/121 (28.93%), Postives = 68/121 (56.20%), Query Frame = 0
Query: 839 LSYMNLTFTLCFSIECVLKLISYG----PTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVF 955
L + + F + F+ ECV+K+++YG +Y ++ WN DF V+ +D L+ + +V LR FR R ++L+ +++++L + + L ++ LL+ + IY+IIG+Q+F
Sbjct: 103 LEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGFMDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMF 223
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000005510 (supercontig:LSalAtl2s:LSalAtl2s297:203090:236595:1 gene:EMLSAG00000005510 transcript:EMLSAT00000005510 description:"maker-LSalAtl2s297-augustus-gene-2.17") HSP 1 Score: 661.759 bits (1706), Expect = 0.000e+0 Identity = 377/636 (59.28%), Postives = 469/636 (73.74%), Query Frame = 0
Query: 110 KKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQT-GLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEE---------ELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQ 735
K+QW++W VI+LV LNTC+VAVEHYNQP WL+ FL+ EF+FLG F+FEM +R++ALG IYF S+FNRFDC+VI+GS+FEV+W + +P+AGSFGLS LRALRLLR+FKVT YWS LRNLV++L++++ SI+SLL LLFLFI IFALLGMQLFGG+FNFPD TP SNFN+ A+LTVFQ+LT EDWN VMY I SQGG G+IYS+YF+ LTL GNY LLNVFLAIA DSL QA E+TA +E EKE +E ++ R + KA A L +EEE + +Q P+LPYSS+FI +STN FR +H VT P FD FIM+VI+ SS +LAAEDPV PRN L YFDY FT +F IE LLKI+D G HPGSYLR++WNV+D LVVSCA+ SF G+ G+ L KS+++LRVLRPLK + RVP LKAVFDCV+ SLKNVFNIL+VY+LF IFA++ VQLFNGKFFYC+D SK +A++CQGEYF++ + P + R+W + F+YDN A++TLFAVQT+EGW VLQ SM++TF D+ P F EM+IFYIVYF+VFPFFFVNIFVAL+I+TF E GE+EL + +IDKNQ
Sbjct: 3 KQQWWFWLVIILVFLNTCTVAVEHYNQPQWLTIFLYYAEFLFLGAFVFEMCIRLYALGPFIYFSSAFNRFDCVVITGSIFEVLWVNLQPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFPDETPTSNFNTIINALLTVFQVLTEEDWNNVMYXAIVSQGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSLDQAAELTAAEEAEKERQEEEQERQRLRE--------------QQELKAIGADGQLGDELRGDEEEGGEPPLPDMDDEDQVRPILPYSSLFILTSTNPFRVGLHWFVTKPFFDGFIMIVILLSSASLAAEDPVEEHHPRNLLLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALISFYFQ--GTPMGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNSMSSTFEDEGPFPWFSTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNELGEAELTD-DIDKNQ 618 HSP 2 Score: 234.572 bits (597), Expect = 3.247e-63 Identity = 148/423 (34.99%), Postives = 229/423 (54.14%), Query Frame = 0
Query: 838 ILSYMNLTFTLCFSIECVLKLISYG----PTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFF-IYAIIGMQVFGNIRF--------------------------DPDTEYN----RHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPS-AEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKE-KIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGRNPLSE 1223
+L Y + FT F+IEC+LK++ G P SY +D WN+ D + V +++ + + + ++L R R +K++ + ++ + V S K + + IL++ MLF I+A++G+Q+F F P E + N+ G+ I+ LF T E W ++ S + D F + +T I +++ + + F +N+FVA+++ F+ L A D+ + S G+I+YT+M+ +LK +DPPLGFGSKCPD LA+K+L+RMN P+D+EG VHF TTLF+L+R NL I MR+ +EMDQAD ELR TI + WP+ ++ K+DLLVPP E G +LTVGKI+GG+LILENWK TKFG P+++
Sbjct: 364 LLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALI-SFYFQGTPMGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNV-FNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNSMSSTFEDEGPFPWFSTEMAI--------------FYIVYFVVFPFFFVNIFVALVIVTFNELGE------AELTDDIDKNQLRQQESWNGGKIHYTDMFALLKQIDPPLGFGSKCPDLLAYKRLVRMNMPVDNEGKVHFNTTLFALVRVNLQIFMRSTDEMDQADQELRTTIGRSWPFTKRDGKLDLLVPPSSETGPNKLTVGKIFGGMLILENWKITKFGSTPVTK 764 HSP 3 Score: 71.2478 bits (173), Expect = 1.608e-12 Identity = 64/243 (26.34%), Postives = 108/243 (44.44%), Query Frame = 0
Query: 818 FFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGF--LRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFD 1058
F + V+++ P LT L Y F F E ++L + GP YF +N FD V + GSI + L V + G LR R R+ K+ + +R L+ + + ++ ++ L+ + FI+A++GMQ+FG PD NF T + ++ +F+ T E W ++M Y A + + G YFV L ++ +LN+F+A+ D+ D
Sbjct: 16 FLNTCTVAVEHYNQPQWLTIFLYYAEFLFLGAFVFEMCIRLYALGPFIYFSSAFNRFDCVVITGSIFEVLWVNLQPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFPDETPTS--NFNTIINALLTVFQVLTEEDWNNVM---------------YXAI---VSQGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSLD 238
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000008334 (supercontig:LSalAtl2s:LSalAtl2s508:27226:174463:1 gene:EMLSAG00000008334 transcript:EMLSAT00000008334 description:"maker-LSalAtl2s508-snap-gene-1.26") HSP 1 Score: 645.58 bits (1664), Expect = 0.000e+0 Identity = 396/762 (51.97%), Postives = 511/762 (67.06%), Query Frame = 0
Query: 42 KRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQ------------AQEMTALQEKEKEDEEAKEAAIRAAY-GPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMM 789
K ++ R L + + E E ++ K+ + KK+F +S+FW E++ RF+IR Y EF+FLG FI E+ +R++ALG R YF ++FN FDC VI GS+ EV+W HFK K GSFGLS LRALRLLR+FKVT YWSSLRNLV++L++++ SI+SLL LLFLFI IFALLGMQ+FGG+FNF GTP SNFN+ + A+LTVFQ+LTGEDWN+VMY I+S+GG + G++YSLYF+ LTL G+YTLLNVFLAIA DSL Q + T L + D E + Y PI K + AE AA AA + +T ++EE ++ P+LPYSS FI SS+N R +IH + T +FD FIM VI+ SSIALA+EDPV+ +S RNK L Y DYGFT +F +E LK+ID G +LHPG+YLR+ WN +D+ VVSCA+ SF + G+ G+ L KSL++LRVLRPLKTI RVPKLKAVFDCV+ SLKNVFNIL+VY+LF IFA++ VQLFNGKFF+C+D+S+ +AD+CQG YF Y ++ P I R W R+ FHYDN A++TLFA+QT +LQ SMA+T+ ++ P +RVEMSIFYIV+F+VFPFFFVNIFVALII+TF E GE+EL++ +DKNQKSCIDFAI A PLE Y+P+ GF Y WR++ S+PFE FIM LI+ NT LLM+
Sbjct: 315 KERVESRVGFMQLKEQQKLEKELNGVQKTHKFIKENEQT--SFKKEFDDDSNFWRSERQFRFFIR-------LY-------------------------------AEFIFLGCFILEILIRLYALGPRTYFSTTFNVFDCFVIVGSIVEVIWVHFKDKGGSFGLSALRALRLLRVFKVTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFSTGTPTSNFNTITSALLTVFQVLTGEDWNQVMYTAIDSKGGRKGGGMVYSLYFVMLTLCGDYTLLNVFLAIACDSLDQAAALTEAEEAEKERHKTTLSDDIFLDMEVHDDKENKKYFDPI------KAAEIAFEKAAEAAAKAATQEITIDQEENIS--IKPILPYSSFFILSSSNPIRVSIHWLXTKKVFDCFIMCVIVLSSIALASEDPVDENSIRNKLLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLDITVVSCALISFYHTLAGTPTGQKL---KSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNVFNILIVYMLFQIIFAIVGVQLFNGKFFFCSDESRTNADECQGYYFHYEVPNE-TPKIVERTWERRSFHYDNCFRAIITLFAIQT------ILQDSMASTYENEGPKPWYRVEMSIFYIVFFVVFPFFFVNIFVALIIVTFNELGEAELQD-NMDKNQKSCIDFAIQARPLELYVPQRTDGFYYHAWRLITSSPFENFIMVLIICNTILLML 1017 HSP 2 Score: 105.145 bits (261), Expect = 5.878e-23 Identity = 169/706 (23.94%), Postives = 270/706 (38.24%), Query Frame = 0
Query: 402 LTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVV-SCAI-----------------------TSFAMDMLGSAG-----GENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPA----IAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITF---QEQGESELEEAEIDKNQKSCIDFAINA-SPLERYMPE-ERVGFR------YRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVG--FLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVF-GNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFD 1058
L E+ E+ P LP SS+F S N R A+ V + +F+ I+ IIA+ I +A + P S + +L + F +F +E+ KI+ G ILH GSYLR WN+MD +VV S +I TSF ++ + G +LST LR RVLRPLK + VP ++ G + + C Y + H P + P +DN+ AMLT+F G +LF IF+I IV FF +N+F+ ++ F +E+ ES + ++ + QK ++ +N +++ E E+ F+ WR F +FI Y +F GCF +E ++ A+ GP +YF +N+FD +VGSIV+ + V F + G LR R R+ K+ + ++R L+ + + ++ ++ L+ + FI+A++GMQ+F G+ F T + NF T S ++ +F+ TGE W +M Y A K K G +S YFV + +LN+F+A+ D+ D
Sbjct: 38 LVMEKSLEMKLHHPNKLP-SSLFFLSGKNPIRRAVIWFVRWIVFEWIIIFTIIANCIVMALDQHLPHKDKSKLSVQLEELEPVFMGIFTMEMCFKIVADGFILHHGSYLRNPWNIMDFIVVISGSIYVHPNKSFIHISHYNDVIYYFYSTSFLPYLIPKSDKKENTGPDLST---LRTFRVLRPLKLVSGVPN----------------------------------RIITG---------GDSGNPC------YRGSSKHSPVGKKWVGP---NNGITSFDNIGLAMLTVFQTNDALG-------------------NLFN---WIFFIPLIIVGSFFMLNLFLGVLSGEFAKEKERVESRVGFMQLKEQQK--LEKELNGVQKTHKFIKENEQTSFKKEFDDDSNFWR--SERQFRFFIRL-------------------------YAEFIFLGCFILEILIRLYAL-------------------------------------GPRTYFSTTFNVFDCFVIVGSIVEVIWVHFKDKGGSFGLSALRALRLLRVFKVTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFSTGTPTS---NFNTITSALLTVFQVLTGEDWNQVM---------------YTAIDSK--GGRKGGGMVYSL-YFVMLTLCGDYTLLNVFLAIACDSLD 578 HSP 3 Score: 55.4546 bits (132), Expect = 8.770e-8 Identity = 41/126 (32.54%), Postives = 67/126 (53.17%), Query Frame = 0
Query: 838 ILSYMNLTFTLCFSIECVLKLISYG----PTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFIN--VGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLF-FIYAIIGMQVFG 956
+L Y + FT F++EC LK+I G P +Y +DFWN D +TVV + + A + L+L R R +K + + ++ + V S K + + IL++ MLF I+AI+G+Q+F
Sbjct: 714 LLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLD-ITVVSCALISFYHTLAGTPTGQKLKSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNV-FNILIVYMLFQIIFAIVGVQLFN 837
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000009889 (supercontig:LSalAtl2s:LSalAtl2s643:800:51309:-1 gene:EMLSAG00000009889 transcript:EMLSAT00000009889 description:"maker-LSalAtl2s643-snap-gene-0.8") HSP 1 Score: 456.447 bits (1173), Expect = 6.562e-145 Identity = 303/591 (51.27%), Postives = 374/591 (63.28%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSD---FWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFH 589
++ + GY WI KA E TTEEER +IMEARKRA KR+K++ LGK RS E+ED+++++E + + A++ + + + FW EK+ RF+IR TVK+QWFYWFVI+LV LNT VAVEHYNQP WL+E L+ EFVFLGLF+ EM ++ + L S+ +VW+ F P L + + + + VSL SM+SIISLL LLFLFILIFALLGMQLFGG FNF DGTPPSNFNSF+I++LTVFQILTGEDWNEVMY GIESQGG ++G++YSLYFI LTLFGNYTLLNVFLAIAVD+L A++ AA +A E +E +AN R +H +VTFP FD FIM++II SSIALAAEDPV +S RN LTYFDY FT +F +E++LK+ID G+I HPGSYLR++WN+MD LVV CA SF DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF
Sbjct: 283 QQQLERELNGYVDWICKAAEER-----TTEEERNHIMEARKRAANKRRKIKVLGKGRSIETEDDDDDDEVDEGISQAS---AIRTRLRNHGGCQPFWRCEKRVRFYIRHTVKQQWFYWFVIVLVFLNTLCVAVEHYNQPEWLTELLYYAEFVFLGLFLAEM-VQGYILN-----------------PHSIVLIVWS-FVP---------------LFLKYAGPNFQVMEDHSVSL--SMRSIISLLFLLFLFILIFALLGMQLFGGAFNFSDGTPPSNFNSFAISLLTVFQILTGEDWNEVMYQGIESQGGHRSGMVYSLYFIILTLFGNYTLLNVFLAIAVDNLAN---------------------------------------------AQELTAAENQA---ENDELVAN----------------AGQIRLFVHGIVTFPFFDTFIMIIIIMSSIALAAEDPVQENSRRNHILTYFDYVFTGIFAVEMILKVIDLGLIAHPGSYLRDVWNLMDALVVICASISFIFDMTGSSKGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVTNILIVYILFQ 765 HSP 2 Score: 79.337 bits (194), Expect = 4.261e-15 Identity = 68/246 (27.64%), Postives = 112/246 (45.53%), Query Frame = 0
Query: 451 LVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMD---------------------MLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDDSKEDADD------CQGEYFLYSATDDHP--PAIAPRL--WRRQKF---HYDNVPTAMLTL 657
L ++ + I LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR WN+MD +VV + +F + + S G +L T LR +RVLRPLK + VP L+ V ++ +L + I ++ + IFA+I ++ ++G K Y ++ + D C + L + H P ++ L W + +DN+ AMLT+
Sbjct: 20 LFLLTNCIVLALEEHLPNGDRTIFAQKLEKTEPYFLGIFTVEAALKILALGFVLHRGSYLRNAWNIMDFIVVVTGMVTFGAEASIPFDLTIVRSFRESEASSSSIPSEEGVDLRT---LRAIRVLRPLKLVSGVPSLQVVLKSILKALAPLMQIALLVMFAILIFAIIGLEFYSGALHKACYSVENLELMVTDGSLKVPCHSDDPLTAPAGAHTCEPNVSICLENWEGPNYGITSFDNIGFAMLTV 262
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000003972 (supercontig:LSalAtl2s:LSalAtl2s2133:21116:38912:-1 gene:EMLSAG00000003972 transcript:EMLSAT00000003972 description:"maker-LSalAtl2s2133-snap-gene-0.4") HSP 1 Score: 398.282 bits (1022), Expect = 1.006e-127 Identity = 194/233 (83.26%), Postives = 210/233 (90.13%), Query Frame = 0
Query: 515 MDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASP 747
MDMLVVSCAITSFAMDMLGS+GGENL TMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVY+LFHFIFAVIAVQLFNGKFFYC+D SK+DA+ +GEYFL+S +D PP + PR+W+RQ+FHYDNV TA+LTLFAVQTTEGWPA+LQQSMAATF DQAPI FRV MSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDK C+ I+ P
Sbjct: 1 MDMLVVSCAITSFAMDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAE--EGEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKKSVECVLKLISYGP 231 HSP 2 Score: 340.502 bits (872), Expect = 3.937e-106 Identity = 178/273 (65.20%), Postives = 185/273 (67.77%), Query Frame = 0
Query: 851 SIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIR 1123
S+ECVLKLISYGP SYFKD WNIFD FV FKALPYVILLIAMLFFIY IIGMQ+FGNI F+P+TEYNRHVNFQTF +GIMVLFRCATGEAWPSIMLACE+G D KYN TSGKILDPEKTCGS+ SY YFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLD EGRIYYTEMYDMLKNMDPPLGF SKC KK IR
Sbjct: 219 SVECVLKLISYGPGSYFKDAWNIFD--------------------------------------------------FVTXFKALPYVILLIAMLFFIYTIIGMQIFGNIEFNPETEYNRHVNFQTFAAGIMVLFRCATGEAWPSIMLACEAGKRXDERNHKYNLTSGKILDPEKTCGSSMSYVYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLD-------------EGRIYYTEMYDMLKNMDPPLGFVSKCEQ---LKKWIR 425 HSP 3 Score: 58.9214 bits (141), Expect = 4.718e-9 Identity = 54/235 (22.98%), Postives = 102/235 (43.40%), Query Frame = 0
Query: 180 DCLVISGSVFEVVWTHFKPKAG-SFG-LSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF-----------------------NFPDGTPPS-------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQT---------GLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGP 373
D LV+S ++ G + G + LR LR+LR K L+ + +++S+K++ ++L++ LF IFA++ +QLF G F +FP P ++++ + A+LT+F + T E W ++ + + Q + Y +YFI F +N+F+A+ + + Q Q + L+E E + + + +YGP
Sbjct: 2 DMLVVSCAITSFAMDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAEEGEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFF----FVNIFVALIIITF-QEQGESELEEAEIDKKSVECVLKLISYGP 231
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000006853 (supercontig:LSalAtl2s:LSalAtl2s3888:1494:5853:1 gene:EMLSAG00000006853 transcript:EMLSAT00000006853 description:"maker-LSalAtl2s3888-augustus-gene-0.2") HSP 1 Score: 360.918 bits (925), Expect = 2.779e-116 Identity = 214/247 (86.64%), Postives = 225/247 (91.09%), Query Frame = 0
Query: 159 MFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-----KAEDAAAAAAR 400
MFLRMWALG RIY+ESSFNRFDCLVISGSVFEVVWTHFKPKAGSFG SVLRALRLLRIFKVT YWSSLRNLV+SLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMY GIESQGGAQ GLIYSLYFIFLT+FGNYTLLNVFLAIAVDSLGQAQEMTA +EKEKEDEEAKEAA+R AYGP+PPGP+T + E++ + ED A AR
Sbjct: 1 MFLRMWALGPRIYYESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGFSVLRALRLLRIFKVTAYWSSLRNLVISLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYQGIESQGGAQAGLIYSLYFIFLTMFGNYTLLNVFLAIAVDSLGQAQEMTACEEKEKEDEEAKEAALRXAYGPVPPGPKTAAQ-EFDEDGNLIVRPEDVEAEEAR 246 HSP 2 Score: 57.7658 bits (138), Expect = 3.302e-9 Identity = 55/206 (26.70%), Postives = 98/206 (47.57%), Query Frame = 0
Query: 856 LKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGF--LRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSF---LMLNLFVAVIMDN 1056
L++ + GP Y++ +N FD + + GS+ + + F + GF LR R R+ K+ ++R L+ + + S K++ ++LL+ + I+A++GMQ+FG PD NF +F ++ +F+ TGE W +M Y E G+ Y + FIFL F +LN+F+A+ +D+
Sbjct: 3 LRMWALGPRIYYESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGFSVLRALRLLRIFKVTAYWSSLRNLVISLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDG--TPPSNFNSFSIAMLTVFQILTGEDWNEVM---------------YQGI-------ESQGGAQAGLIYSLYFIFLTMFGNYTLLNVFLAIAVDS 184
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000008332 (supercontig:LSalAtl2s:LSalAtl2s508:183886:232439:1 gene:EMLSAG00000008332 transcript:EMLSAT00000008332 description:"maker-LSalAtl2s508-snap-gene-1.28") HSP 1 Score: 309.301 bits (791), Expect = 3.051e-96 Identity = 147/224 (65.62%), Postives = 185/224 (82.59%), Query Frame = 0
Query: 995 MLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
MLA SG +CD A +YN TSG +++ E+ CGS+ +Y YF+SFIF CSF+MLNLFVAVIMDNFDYLTRD+SILG+HHL EFI W+E+DPS GRI+YTE++++L+++DPPLGFG KCP+R+AFKKLIRMN P+D EG VHFTTTLF+LIRE+L I +R + EMDQAD+ELR TIIK WP+ ++KIDLLVP + GKG LTVGK+YGG++I NW TKFG+
Sbjct: 1 MLAVLSGRSCDLKALEYNITSGTLINQEQNCGSSLTYIYFISFIFFCSFIMLNLFVAVIMDNFDYLTRDTSILGSHHLGEFITTWSEFDPSGIGRIHYTEVFNLLRSIDPPLGFGKKCPERMAFKKLIRMNMPMDTEGKVHFTTTLFALIREHLKINVRESSEMDQADIELRTTIIKCWPHTKRDKIDLLVPTAKSTGKGYLTVGKLYGGVMIHNNWCNTKFGQ 224
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000008678 (supercontig:LSalAtl2s:LSalAtl2s537:391902:427693:-1 gene:EMLSAG00000008678 transcript:EMLSAT00000008678 description:"maker-LSalAtl2s537-snap-gene-3.43") HSP 1 Score: 288.5 bits (737), Expect = 1.131e-86 Identity = 159/304 (52.30%), Postives = 177/304 (58.22%), Query Frame = 0
Query: 868 KDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIK 1171
+D WN FDF+TVVGSIVDALMVEFA ALPYV LL+AMLFFIY IIGMQVFGNI+ DP+ CATGEAWPSIML+CE G CDP A N T+G+I+D K CGS F+Y YFVSFIFLCSFLMLNLF+ YD MLKNMDPPLGFGSKCPDRLA+KKLIRMN P++ EG V+FTTTLF+LIRENLSIKMRAAEEMDQAD+ELR TI+K
Sbjct: 38 QDPWNTFDFITVVGSIVDALMVEFA---------------------------------------ALPYVCLLMAMLFFIYTIIGMQVFGNIKLDPE---------------------CATGEAWPSIMLSCEDGRDCDPRALHINETTGEIVDKTKKCGSGFTYVYFVSFIFLCSFLMLNLFM-------------------------------YD--------------MLKNMDPPLGFGSKCPDRLAYKKLIRMNMPLNSEGKVNFTTTLFALIRENLSIKMRAAEEMDQADVELRDTILK 236 HSP 2 Score: 53.1434 bits (126), Expect = 3.374e-7 Identity = 27/31 (87.10%), Postives = 30/31 (96.77%), Query Frame = 0
Query: 705 FFVNIFVALIIITFQEQGESELEEAEIDKNQ 735
FFVNIFVALIIITFQEQGE+EL++ EIDKNQ
Sbjct: 8 FFVNIFVALIIITFQEQGEAELQDGEIDKNQ 38
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000009714 (supercontig:LSalAtl2s:LSalAtl2s627:140867:158129:1 gene:EMLSAG00000009714 transcript:EMLSAT00000009714 description:"maker-LSalAtl2s627-snap-gene-1.23") HSP 1 Score: 228.024 bits (580), Expect = 8.248e-61 Identity = 229/939 (24.39%), Postives = 392/939 (41.75%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHYNQPH--------WLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF------NFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVF------------------------LAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPP---------------GPRTKERIEYERRKAEDAAAAAARALTAEEEE---------ELANQAPP----VLPYSSMF--IFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED------PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAI--APRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQ---GESELEEAEIDKNQKSCI--------DFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDP--DTEYN 967
+ L +++N + +HYN + ++ L + F +F FE F+++ A+ R +F +N FD L++ S+ E++ G GLS+LR+ RLLR+FK+ W SL +++ + +++ ++ +L ++L + I IFA++GMQLFG + + P NF F + + VF++L GE W E M+ + G YF+ L N +LN+F ++IAV+ + T + KE+ + + P+ P G E + D + T + EE E+ + PP ++P + + R ++ F+ ++ +I+ SS + ED PV D L Y D T VF +E LK+ G P Y W +D ++V+ ++ +F ++G+ N+ K++R LR LRPL+ + ++ +K V + +I +L ++FN+L+V ++F IFA+I V LF G F C D G F + + + W K +DNV A L L V T +GW ++ ++ + ++ P + M +++ + I+ FF +N+ V +II F EQ G S L +A + ++QK I + A P + P+ V + IV + F+ IM I LN +MM +YQQ + L +N F FT+E K+ A+ Y P WN+FDF V+ S L L + IR LL++ V + AL + LL+ ++ FI+A+ GM +F N++ P D E++
Sbjct: 630 ITLCIVVNVLFMMFDHYNIEYDANGGMSMEMNNLLKNGNYFFTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILSLVELL------AXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGSFYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGE-WIESMWACMYVAGPTCVP-----YFLATVLVANLVILNLFLALLLSSFSDMGGSGEEEDGKPDKMSIAVNRFKRLFSFTKRKIKEELFDRIXRKIAHSKSKPLIPEANGNGFSHKTNGYIGGHNNVPSETQTYIPIDQQSDKFAPQTKDHEEIYYDPLETLEVEDCCPPFVYRIIPLGDQHSPFWEAWTQQRLMAMRLIENEYFEGVVLGLILLSSFVMTLEDIWFETRPVLIDC-----LYYLDRILTVVFFLETTLKLFAMG----PVMYFTNAWCWLDFVIVAXSLINFIASLVGAV---NIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCI-------DSIXGHKFSHEIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNEQKNKGGSSL-DAFMTEDQKKYIAAMKKASKKKPLKALPRPYWKPQAIV------FGIVTNKKFDMIIMGFIGLNMLTMMMDHYQQTEGWTFVLDNLNVFFIMIFTLEMLLKIFALRQYYFSEP--------------------------------------WNLFDFFIVLLS--------------------------LGGLFLRAKGIRTLLFSLVMALPALGNICLLLFLVMFIFAVFGMSLFKNVKIIPGFDDEFD 1466 HSP 2 Score: 85.1149 bits (209), Expect = 8.270e-17 Identity = 55/178 (30.90%), Postives = 91/178 (51.12%), Query Frame = 0
Query: 835 LTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNR----HVNFQTFGSGIMVLFRCATGEAWPSIMLACE--SGSTCDP 1006
+ ++L N FT F+ E +KL++ P +F D WN FDF+ V+ S+V+ L A + LR FR R+ KL + ++ ++ + AL + +++ ++ FI+A++GMQ+FG+ + E + NF F M++FR GE W M AC +G TC P
Sbjct: 660 MNNLLKNGNYFFTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILSLVELL----AXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGSFYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGE-WIESMWACMYVAGPTCVP 832 HSP 3 Score: 75.485 bits (184), Expect = 7.422e-14 Identity = 53/170 (31.18%), Postives = 90/170 (52.94%), Query Frame = 0
Query: 433 RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG 602
R AI V+T PLF+ I+ I+ + I + P + + + FT ++ E +K++ G ILH +YLR+ WN +D V+ + + A+D L + SLR RV R LK++ VP LK + +I S+KN+F++ ++ L +FA++ +QL+ G
Sbjct: 158 RIAIR-VLTHPLFNFIIICTILVNCIVMML--------PDTEYXSASEIIFTSIYTYESAVKLLARGFILHNFTYLRDPWNWLDFAVIGMSYITIAID---------LGSFSSLRTFRVFRALKSVAXVPGLKTIVGAIIYSVKNLFDVTILTLFGLALFALLGLQLYMG 309 HSP 4 Score: 74.3294 bits (181), Expect = 1.913e-13 Identity = 66/311 (21.22%), Postives = 146/311 (46.95%), Query Frame = 0
Query: 90 KNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEH--YNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSV---LRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN---------------FPDGTPPSN------------FNSFSIAMLTVFQILTGEDWNEVMYHGIES-----QGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEM--TALQEKEKEDEE 361
++S FW + R + ++ ++F V+ L+LL++ + +E + L + L+ + + +F E L+++A+G +YF +++ D ++++ S+ + + G+ + + +R LR LR + ++ +V +L+ ++ SI ++LL+ +F LIFA++G+ LF GTF+ P+ T N F++ +A L + Q+ T + W +M I+S Q + + YF+ + G++ LN+ + + +D + + ++L ED++
Sbjct: 989 QHSPFWEAWTQQRLMAMRLIENEYFEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMGPVMYFTNAWCWLDFVIVAXSLINFIAS----LVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDSIXGHKFSHEIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNEQKNKGGSSLDAFMTEDQK 1295
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|60391851|sp|P91645.3|CAC1A_DROME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Protein cacophony; AltName: Full=Protein nightblind A; AltName: Full=Protein no-on-transient B; Short=Dmca1A) HSP 1 Score: 1509.58 bits (3907), Expect = 0.000e+0 Identity = 801/1235 (64.86%), Postives = 926/1235 (74.98%), Query Frame = 0
Query: 5 FSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDE----EEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFA---KNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDP-DTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
F A Y WI +AEEVIL E+RTTEEE+ +IMEAR+R KRKKL++LGKS+ST++E+E + ++ L + + G + FW EK+ RFWIR TVK QWFYWFVI+LV LNT VAVEHY QP +L+EFL+ EF+FLGLF+ EMF++M+ALG RIYFESSFNRFDC+VISGS+FEV+W+ K GSFGLSVLRALRLLRIFKVT YWSSLRNLV+SLL+SM+SIISLL LLFLFILIFALLGMQLFGG FN P GTP +NFN+F IA+LTVFQILTGEDWNEVMY GI SQGGAQ G+IYS+YFI L LFGNYTLLNVFLAIAVD+L AQE+TA LQE EKE E + + G + A + + +EEEE+ P+LPYSSMFI S TN R H VV P FD FIM+VI SSIALAAEDPV +S RNK L YFDY FT VF IE+LLKI+D GVILHPGSYLRE WN+MD +VV CA SF DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIF+VI VQLFNGKFFYCTD+SK + +CQG YF Y D+ P R+W+ + FHYDNV AMLTLFAVQT EGWP VLQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ R F+Y++WRIV STPFEYFIM LIV NT LLMMKY H + L Y+N+ FT FS+E VLK+I +G ++FKD WNIFD +TV+GSIVDAL +EF N INVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI+ + RH NFQ+F G+M+LFRCATGEAWP+IMLAC G CD A K P + CGST +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+DDE V FTTTLF+LIRENLSIKMRA EEMDQAD+ELR+TI +WP + K+ ++LLVPP +++ KG+L+VGKIY G LILE+W++T+FG+
Sbjct: 338 FQTAMVSYLDWITQAEEVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSC--------TGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK--GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENG--------------DGAVAPSKSKGKKKEEEKKEEEEVTEGPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEE-DELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKY-------------------------------------HNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEK---------APGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWPLQAKKMLNLLVPPSDQLNKGKLSVGKIYAGFLILESWRSTRFGQ 1501 HSP 2 Score: 181.415 bits (459), Expect = 9.780e-45 Identity = 176/704 (25.00%), Postives = 306/704 (43.47%), Query Frame = 0
Query: 413 QAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDDSK------EDADDCQGEYFLYSATD----DHPPAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQ---------KSCIDFAINASPL----ERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLM----------MKYYQQPVMMSVGL------------HYMNTVFTGCFTVECFF-KVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFD-----PDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNF 1057
+ PP +S+FI + N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F C+F +E LKI+ G++LH SYLR +WN+MD VV +T F M G E +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C + L AT ++ ++ W +DN+ AMLT+F T EGW A+L + + A S F I+++ ++ FF +N+ + ++ F + E K + S +D+ A + ER EE++ R + +T +K +P G H + T + F + F + + V+++G P LT+ L Y F F E +K+ + GP YF+ +N FD V + GSI + + E + LR R R+ K+ + ++R L+ + + S +++ ++ L+ + I+A++GMQ+FG +F+ P+T NF TF ++ +F+ TGE W +M G I G +S YF+ + ++ +LN+F+A+ +DN
Sbjct: 8 ENPPGGGPTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVV---VTGF-MTQYPQIGPE--VDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILY------WTNDALGSAFNW---IYFVPLIVIGSFFMLNLVLGVLSGEFSNERNRVERRMEFQKCRFRAMFQTAMVSYLDWITQAEEVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGG-QFNLPGGTPET------NFNTFPIALLTVFQILTGEDWNEVMY------------------QGIISQGGAQKGMIYS-IYFIVLVLFGNYTLLNVFLAIAVDNL 670
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|322518442|sp|C9D7C2.1|CAC1A_APIME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Cacophony protein) HSP 1 Score: 1474.53 bits (3816), Expect = 0.000e+0 Identity = 786/1227 (64.06%), Postives = 934/1227 (76.12%), Query Frame = 0
Query: 12 YFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA------LQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHP--PAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR-----NPLSEAQ 1225
Y WI KAEEVIL E+RTTEEE+++I+E RKRA K+KKL + + E E ++++++ LS + G FW+ EK+ R+WIRK+VK Q FYWFVI+LV NT VAVEHY QP WL++FL+ EFVFL LF+ EMF++++ALG R YF+SSFNRFDC+VISGS+FEV+W+ K +GSFGLSVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQGG + G+IYSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A ++K+K+ +E E I++ P G ++E + ++ +E+++ + P+LPYSSMFI S TN R A H VV FD FIM+VI SSIALAAEDPV DSPRN+ L YFDY FT VF +E++LKIID G+ILHPGSYLRE WN+MD +VV CA SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNGKFFYC+D+SK DCQG+YF++ +D P R W+ Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK+++Q Y NT L YMN+ FT F++EC+LK+ ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT +H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A +GK E CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D + V+FTTTLF+LIRENL+IK+R A E +QA+ ELR TI +WP + K+ +DLL+P EEIG+G++TVGKIY LLILE+W+ T+FG+ P+ E Q
Sbjct: 476 YLNWICKAEEVILAEERTTEEEKKHILEGRKRAEAKKKKLGKSKSTDTEEEEGDDDQDDGELSSSTKEKGPC--------KQFWLAEKRFRYWIRKSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSEVK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFDSGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENEEEEEDKQKQAQEI-EKEIQSLQNPKDGG---APKVEICPPNGKGGKQSSEEEKKQDEDDDTGPK--PMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTQQDCQGQYFVF---EDGALLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDA------YKNT-------------------------------LKYMNMCFTGMFTVECILKIAAFGVRNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSITKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCVKGRPCD-------AKAGK---QEGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDVDLKVNFTTTLFALIRENLNIKVRRASERNQANEELRDTIRSIWPLQAKKMLDLLIPRNEEIGRGKMTVGKIYVCLLILESWRTTRFGQIESAGQPIMELQ 1636 HSP 2 Score: 124.405 bits (311), Expect = 2.790e-27 Identity = 95/325 (29.23%), Postives = 156/325 (48.00%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCA-ITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDC----QGEYFLYSATDDHPPAIAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD VV IT+F S G E +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y D E A C + E + D + W F +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 146 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKQDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAF------SQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRDINVIVKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 452 HSP 3 Score: 72.7886 bits (177), Expect = 1.771e-11 Identity = 80/347 (23.05%), Postives = 147/347 (42.36%), Query Frame = 0
Query: 98 EKKARFWIRKTVKKQW--FYWFVILLVLLNTCSVAVEHY---NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFP--------------DGTPPSN----------------------------------FNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYE 387
K RF I +W F + V+L ++ N +A+E + L++ L TE FLG+F E L++ ALG + Y + +N D V+ V + + L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT + + P N F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E+E
Sbjct: 158 RKHTRFII------EWPPFEYAVLLTIIANCVVLALEEHLPKQDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVV---VTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRDINVIVKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLEHE 472
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|116411|sp|P15381.1|CAC1C_RABIT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Smooth muscle calcium channel blocker receptor; Short=CACB-receptor; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 958.748 bits (2477), Expect = 0.000e+0 Identity = 562/1281 (43.87%), Postives = 753/1281 (58.78%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVIL-NEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWV-YEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPD-GTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQ------------------EKEKED------EEAKEAAIR----AAYGPIPPGPRTKERIEYERRKAEDAA--------------------AAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF---------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
K+ + +GY WI +AE++ NED +EE+ M + TES + E + + LA + K S +W + + R R VK FYW VI LV LNT ++A EHYNQPHWL+E T L LF EM L+M++LGL+ YF S FNRFDC ++ G + E + K + G+SVLR +RLLRIFK+T YW+SL NLV SLL+S++SI SLLLLLFLFI+IF+LLGMQLFGG FNF + T S F++F ++LTVFQILTGEDWN VMY GI + GG G++ +YFI L + GNY LLNVFLAIAVD+L A+ +T+ Q EK++E EEAKE I A G PP + + + ++ED + R L+ E L +A P+ S+ FIFS N FR H +V +F I+ I+ SSI+LAAEDPV S RN L YFD FT +F IE+ LK+ YG LH GS+ R +N++D+LVVS ++ SF + ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GK + C+D SK+ +C+G Y Y + P I PR W KF +DNV AM+ LF V T EGWP +L +S+ + D+ PI +RVE+SIF+I+Y I+ FF +NIFV +I+TFQEQGE E + E+DKNQ+ C+++A+ A PL RY+P+ + +Y++W +V ST FEY + LI+LNT L M++Y Q + + ++ +N +FTG FTVE +LKLI++ P YF D WN+FDF+ V+GSI+D ++ E + I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLI MLFFIYA+IGMQVFG I + TE NR+ NFQTF +++LFRCATGEAW IMLAC G C P + +N+T G E CGS+F+ YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK ++QA+ ELR I K+W + +D +VPP G +TVGK Y LI E ++ K
Sbjct: 457 KQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSM---------------PTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKVMS-PLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMD-DLQPNESEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLS---ELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQ------SSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQ--HQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVE-------------------------------------MILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPHNSTEG-----ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEG--NLEQANEELRAIIKKIWKRTSMKLLDQVVPP---AGDDEVTVGKFYATFLIQEYFRKFK 1662 HSP 2 Score: 173.326 bits (438), Expect = 3.553e-42 Identity = 175/735 (23.81%), Postives = 302/735 (41.09%), Query Frame = 0
Query: 419 PYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLS-------TMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPA--------------IAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFV-NIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINA---------------SPLERYMPE------------------ERVGFRY----------RLWR------------IVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF-AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNF 1057
P ++ + N R A ++V + F++ I+L I A+ +ALA P D N L +Y F +F +E LK+I YG++ HP +YLR WN++D ++V + S ++ A G N +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A+I ++LF GK + + AD A DD P + W K ++DN AMLT+F T EGW VL Q +M E+ Y V ++F FFV N+ + ++ F ++ E E+ +++++ K +D+ A P MP E G R R WR V+S F + ++ L+ LNT + ++Y QP ++ N FT E K+ ++G++ +YF +N FD V G I++ ++VE + + + LR R R+ K+ R ++ L+ + + S +++ ++LL+ + I++++GMQ+FG +F+ D R F F ++ +F+ TGE W S+M A G C YF+ +F+C ++++LN+F+A+ +DN
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVAD--------VPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQ-------------------------------------AYFVSLFNRFDCFIVCGGILETILVETKVMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGG-KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYD------------GIMAYGGPSFPGMLVC------IYFI-ILFICGNYILLNVFLAIAVDNL 786 HSP 3 Score: 107.457 bits (267), Expect = 4.089e-22 Identity = 157/684 (22.95%), Postives = 285/684 (41.67%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHY-----NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGL----RIYFESSFNRFDCLVISGSVFEVVWTH---------FKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF-----------NFPDGTPPS------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSL---YFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMT-------ALQEKEKEDEEAKEAA--IRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEE----EEELANQAPPVLPYSSMFIFSST----NSF-RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAI------TSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALII 715
+ILL + C VA+ Y + + + L E++FL +F E FL++ A GL Y + +N D +++ +F + K F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + P PS NF++F+ AMLTVFQ +T E W +V+Y ++ G Y L YF+ L +FG++ +LN+ L + + +E L+EK++ +E+ K I A P E ++ E+ + + ++ E + E N + S FS N F R V +F ++ ++ +++ +A+E N + + +F E+LLK+ G+ +Y L+N D +V I + M LG + LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF + D+ Q RR F DN P ++LT+F + T E W +V+ + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 159 IILLTIFANC-VALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPEN--EDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKVMSPLG---------ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF---------DEMQT--------------------RRSTF--DNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 783
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|6165982|sp|Q01815.1|CAC1C_MOUSE (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=MELC-CC; AltName: Full=Mouse brain class C; Short=MBC; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 953.74 bits (2464), Expect = 0.000e+0 Identity = 558/1277 (43.70%), Postives = 748/1277 (58.57%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEV-ILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWV-YEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPD-GTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQ------------------EKEKED------EEAKEAAIR----AAYGPIPPGPRTK----ERIEYERRKAEDAAAAAARALTAE------------EEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF---------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
K+ + +GY WI +AE++ NED +E++ M + TES + E + + LA + K S +W + + R R VK FYW VI LV LNT ++A EHYNQPHWL+E T L LF EM L+M++LGL+ YF S FNRFDC ++ G + E + K + G+SVLR +RLLRIFK+T YW+SL NLV SLL+S++SI SLLLLLFLFI+IF+LLGMQLFGG FNF + T S F++F ++LTVFQILTGEDWN VMY GI + GG G++ +YFI L + GNY LLNVFLAIAVD+L A+ +T+ Q EK++E EE+KE I A G PP + + E E + A E E L +A P+ S+ FIFS N FR H +V +F I+ I+ SSI+LAAEDPV S RN L DY FT +F +E++LK+ YG LH GS+ R +N++D+LVVS ++ SF + ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GK + C+D SK+ +C+G Y Y + P I PR W KF +DNV AM+ LF V T EGWP +L +S+ + D+ PI +RVE+SIF+I+Y I+ FF +NIFV +I+TFQEQGE E + E+DKNQ+ C+++A+ A PL RY+P+ + +Y++W +V ST FEY + LI+LNT L M++Y Q + + ++ +N +FTG FTVE +LKLI++ P YF D WN+FDF+ V+GSI+D ++ E + I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLI MLFFIYA+IGMQVFG I + TE NR+ NFQTF +++LFRCATGEAW IMLAC G C P + N+T G E CGS+F+ YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK ++QA+ ELR I K+W + +D +VPP G +TVGK Y LI E ++ K
Sbjct: 427 KQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSM---------------PTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMS-PLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQ------SSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQ--HQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVE-------------------------------------MILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-----ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEG--NLEQANEELRAIIKKIWKRTSMKLLDQVVPP---AGDDEVTVGKFYATFLIQEYFRKFK 1632 HSP 2 Score: 172.94 bits (437), Expect = 4.513e-42 Identity = 174/729 (23.87%), Postives = 303/729 (41.56%), Query Frame = 0
Query: 419 PYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLS-------TMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFF-YCTDD-------SKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFV-NIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINA---------------SPLERYMPE------------------ERVGFRY----------RLWR------------IVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFA-KNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNF 1057
P ++ + N R A ++V + F++ I+L I A+ +ALA P D N L +Y F +F +E LK+I YG++ HP +YLR WN++D ++V + S ++ A G N +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A+I ++LF GK C + ++ED C E + + W K ++DN AMLT+F T EGW VL Q +M E+ Y V ++F FFV N+ + ++ F ++ E E+ +++++ K +D+ A P MP E G R R WR V+S F + ++ L+ LNT + ++Y QP ++ N FT E K+ ++G++ +YF +N FD V G I++ ++VE + + + LR R R+ K+ R ++ L+ + + S +++ ++LL+ + I++++GMQ+FG +F+ D R F F ++ +F+ TGE W S+M A G C YF+ +F+C ++++LN+F+A+ +DN
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNG--TVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQ-------------------------------------AYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGG-KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYD------------GIMAYGGPSFPGMLVC------IYFI-ILFICGNYILLNVFLAIAVDNL 756 HSP 3 Score: 106.301 bits (264), Expect = 9.407e-22 Identity = 156/680 (22.94%), Postives = 285/680 (41.91%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHY-----NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGL----RIYFESSFNRFDCLVISGSVFEVVWTH---------FKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLF-----------GGTFNFPDGTPPS------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSL---YFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMT-------ALQEKEKEDEEAKEAA--IRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEE----EEELANQAPPVLPYSSMFIFSST----NSF-RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAI--TSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALII 715
+ILL + C VA+ Y + + + L E++FL +F E FL++ A GL Y + +N D +++ +F + K F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + P PS NF++F+ AMLTVFQ +T E W +V+Y ++ G Y L YF+ L +FG++ +LN+ L + + +E L+EK++ +E+ K I A P E ++ ++ + + ++ E + E N + S FS N F R V +F ++ ++ +++ +A+E N + + +F E+LLK+ G+ +Y L+N D +V I T + S G + LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF + D+ Q RR F DN P ++LT+F + T E W +V+ + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 129 IILLTIFANC-VALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPEN--EDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKIMSPLG-----ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF---------DEMQT--------------------RRSTF--DNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 753
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|308153651|sp|Q13936.4|CAC1C_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 944.88 bits (2441), Expect = 0.000e+0 Identity = 565/1301 (43.43%), Postives = 755/1301 (58.03%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEV-ILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWV-YEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPD-GTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQ----------------EKEKEDE--------EAKEAAIR----AAYGPIPPGPRTKERIEYERRKAEDAA--------------------AAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFD--------------------YGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF---------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
K+ + +GY WI +AE++ NED +EE+ M + TES + E + + LA + K S +W + + R R VK FYW VI LV LNT ++A EHYNQP+WL+E T L LF EM L+M++LGL+ YF S FNRFDC V+ G + E + K + G+SVLR +RLLRIFK+T YW+SL NLV SLL+S++SI SLLLLLFLFI+IF+LLGMQLFGG FNF + T S F++F ++LTVFQILTGEDWN VMY GI + GG G++ +YFI L + GNY LLNVFLAIAVD+L A+ +T+ Q EK+ E E+KE I A G PP + + + + ED + R L+ E L +A P+ S+ FIFSS N FR H +V +F I+ I+ SSI+LAAEDPV S RN L YFD Y FT +F +E++LK+ YG LH GS+ R +N++D+LVVS ++ SF + ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GK + C+D SK+ +C+G Y Y + P I PR W KF +DNV AM+ LF V T EGWP +L +S+ + D+ PI +RVE+SIF+I+Y I+ FF +NIFV +I+TFQEQGE E + E+DKNQ+ C+++A+ A PL RY+P+ + +Y++W +V ST FEY + LI+LNT L M++Y Q + + ++ +N +FTG FTVE K+IA K + + P F I ++ +I YF D WN FD + VVGSIVD + E + I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLI MLFFIYA+IGMQVFG I + TE NR+ NFQTF +++LFRCATGEAW IMLAC G C P + N+T G E CGS+F+ YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK ++QA+ ELR I K+W + +D +VPP G +TVGK Y LI E ++ K
Sbjct: 427 KQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSM---------------PTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMS-PLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMD-DLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLS---ELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQ------SSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQ--HQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPW---------NVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-----ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEG--NLEQANEELRAIIKKIWKRTSMKLLDQVVPP---AGDDEVTVGKFYATFLIQEYFRKFK 1680 HSP 2 Score: 173.326 bits (438), Expect = 3.388e-42 Identity = 172/731 (23.53%), Postives = 300/731 (41.04%), Query Frame = 0
Query: 419 PYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLS-------TMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPA--------------IAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINA---------------SPLERYMPE------------------ERVGFRY----------RLWR------------IVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFA-KNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNF 1057
P ++ + N R A ++V + F++ I+L I A+ +ALA P D N L +Y F +F +E LK+I YG++ HP +YLR WN++D ++V + S ++ A G N +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A+I ++LF GK + + AD A DD P + W K ++DN AMLT+F T EGW VL A D I+++ I+ FF +N+ + ++ F ++ E E+ +++++ K +D+ A P MP E G R R WR V+S F + ++ L+ LNT + ++Y QP ++ N FT E K+ ++G++ +YF +N FD V G I++ ++VE + + + LR R R+ K+ R ++ L+ + + S +++ ++LL+ + I++++GMQ+FG +F+ D R F F ++ +F+ TGE W S+M A G C YF+ +F+C ++++LN+F+A+ +DN
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAD--------VPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWP---------WIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQ-------------------------------------AYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGG-KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYD------------GIMAYGGPSFPGMLVC------IYFI-ILFICGNYILLNVFLAIAVDNL 756 HSP 3 Score: 104.375 bits (259), Expect = 3.834e-21 Identity = 152/679 (22.39%), Postives = 281/679 (41.38%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHY-----NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGL----RIYFESSFNRFDCLVISGSVFEVVWTH---------FKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTF-----------NFPDGTPPS------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMT-------ALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEE----EEELANQAPPVLPYSSMFIFSST----NSF-RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAI------TSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALII 715
+ILL + C VA+ Y + + + L E++FL +F E FL++ A GL Y + +N D +++ +F + K F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + P PS NF++F+ AMLTVFQ +T E W +V+Y ++ G +YF+ L + G++ +LN+ L + + +E L+EK++ +E+ K E ++ E+ + + ++ E + E N + S FS N F R V +F ++ ++ +++ +A+E N + + + +F E+LLK+ G+ +Y L+N D VV I + M LG + LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF + D+ Q RR F DN P ++LT+F + T E W +V+ + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 129 IILLTIFANC-VALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPW----IYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFVVCGGILETILVETKIMSPLG---------ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF---------DEMQT--------------------RRSTF--DNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 753
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|1209676875|sp|Q02485.2|CAC1S_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=ROB1; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 929.858 bits (2402), Expect = 0.000e+0 Identity = 519/1171 (44.32%), Postives = 712/1171 (60.80%), Query Frame = 0
Query: 102 RFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDG-TPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEK---------------EKEDEEAKEAAIRAAYGP----IPPGPRTK-ERIEYERRKAEDAAAAAARALTAEEEE---------------ELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF-----------------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWK 1212
R+ VK + FYW VIL+V LNT S+A EH+NQP WL+ V L LF EM ++M+ LGLR YF S FNRFDC V+ + E++ G+SVLR +RLLR+FK+T YW+SL NLV SLL+S++SI SLLLLLFLF++IFALLGMQLFGG ++F D SNF++F A+++VFQ+LTGEDWN VMY+GI + GG G++ +YFI L + GNY LLNVFLAIAVD+L +A+ +T+ Q+ +K +EE + P IP + K + E + +D +A EE+E +L +A P+ SS FIFS TN R H +V F FI+L I+ SS ALAAEDP+ DS RN+ L YFDY FT VF +E++LK+ YG LH GS+ R +N++D+LVV+ ++ S ++ +S +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GKF+ C D SK ++C+G Y++Y D + PR W FH+DNV +AM++LF V T EGWP +L +++ + D P+ RVEM+IF+I+Y I+ FF +NIFV +I+TFQEQGE+E + E+DKNQ+ C+ +A+ A PL Y+P+ ++Y++W +V S+ FEY + LI+LNT L M++Y Q M+ H ++DIL N+ FT+ F++E +LKLI++ P YF D WN+FDF+ V+GSI+D ++ E I+ F RLFR RL+KLL + +R LLWTF++SF+ALPYV LLI MLFFIYA+IGMQ+FG I T+ NR+ NFQTF +++LFRCATGEAW I+LAC G CDP + + G+ E CG+ F+Y YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF IWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK +QA+ ELR I K+W + +D ++PP IG +TVGK Y LI E+++
Sbjct: 422 RWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVE-SGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLE------SSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKN--PYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMN-----------------------------H-----ISDIL---NVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKRCDPES---DYAPGE----EYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEG--NFEQANEELRAIIKKIWKRTSMKLLDQVIPP---IGDDEVTVGKFYATFLIQEHFR 1534 HSP 2 Score: 179.104 bits (453), Expect = 4.668e-44 Identity = 174/715 (24.34%), Postives = 310/715 (43.36%), Query Frame = 0
Query: 415 PPVLPYS--SMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLG---------SAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGK-----FFYCTD----DSKEDADDC----QGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE--------------SELEE--------------AEIDKNQKSCIDFAINASPLE---------------RYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF-AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFD-PDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNF 1057
P +LP ++F + N R A +VV + F+ I+L I A+ +ALA P+ D N L +Y F VF IE +KII YG + H +YLR WNV+D ++V + + ++ + S+ G L +K+LR RVLRPL+ + VP L+ V + + ++ +F+I ++ L I+A+I ++LF GK +F TD E C G + ++ P H+DN +MLT++ + EGW VL A ++ P I+++ ++ FF +N+ + ++ F ++ E +LEE ++D ++ + S E R+ + FR++ +V+S F + ++ ++ LNT + +++ QP+ ++ N V FT+E K+ +G++ YF +N FD V I++ L+VE A + + LR R RL K+ + ++ L+ + + S +++ ++LL+ + I+A++GMQ+FG R+D DTE R NF F ++ +F+ TGE W S+M YN G + + YF+ +F+C ++++LN+F+A+ +DN
Sbjct: 21 PEILPRPPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLD-VKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGP---NHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDA-IGNEWPW--------IYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLR-------------------------------------QYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGG-RYDFEDTEVRRS-NFDNFPQALISVFQVLTGEDWNSVM---------------YN---GIMAYGGPSYPGVLVCIYFI-ILFVCGNYILLNVFLAIAVDNL 664 HSP 3 Score: 113.235 bits (282), Expect = 8.483e-24 Identity = 160/734 (21.80%), Postives = 308/734 (41.96%), Query Frame = 0
Query: 64 DEEEEEEELLSKKKRAP--GLALKKK---FKKNSDFWVYE--KKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWL-SEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFES----SFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN-------------------FPDGTPPS-------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIY------SLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMD----MLGSA--------GGENLSTMKSLRV-------LRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATF-------ADQAPISLFRVEMSI---FYIVYFIVFPFFFVNIFVALIIITF 718
D+EE+E E+ + + P L LK+K + S F+++ K R + V WF F++L +LL++ ++A E + + ++ L ++VF +F E+ L+M G ++ S FN D LV++ S+ + + A S + +LR LR+LR + L+++V + ++++I +++L+ L +FA +G+QLF G F + DG P +F++ AM+++F + T E W +++Y I+S TG +Y +++FI + + ++N+F+ + + Q Q T + E + + ++ ++Y A AR L P PY + T+S+ F+ + +I+ ++I L + N N + FT +F +E++LK+I + P Y + WNV D L+V +I + +L S+ G N+ +S R+ RV+R +K + R ++ + I S + + + ++ ++ FI+AVI +Q+F G+ + T ++ R F P A+L LF T E W +L +A ++ +D AP + + ++I ++++ F +N+FVA+I+ F
Sbjct: 744 DDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISM---GLESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSN-EEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTF-QEQGETEYKNCELDKNQ-------------------RQCVQY---------ALKARPLRC---------YIPKNPYQYQVWYVVTSSY------------FEYLMFALIMLNTICLGMQH-YNQSEQMNHISDILNVAFTIIFTLEMILKLIAF----KPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMF-------------------GKIAMVDGT---------QINRNNNFQ--TFPQAVLLLFRCATGEAWQEIL---LACSYGKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNF 1384
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|116412|sp|P22002.1|CAC1C_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Rat brain class C; Short=RBC; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 927.546 bits (2396), Expect = 0.000e+0 Identity = 555/1277 (43.46%), Postives = 739/1277 (57.87%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEV-ILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWV-YEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPD-GTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQ------------------EKEKED------EEAKEAAIR----AAYGPIPPGPRTK----ERIEYERRKAEDAAAAAARALTAE------------EEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF---------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
K+ + +GY WI +AE++ NED +E++ M + TES + E + + LA + K S +W + + R R VK FYW VI LV LNT ++A EHYNQPHWL+E T L LF EM L+M++LGL+ YF S FNRFDC ++ G + E + K + G+S R +RLLRIFK+T YW+SL NLV SLL+S++SI SLLLLLFLFI+IF+LLGMQLFGG FNF + T S F++F ++LTVFQILTGEDWN VMY GI + GG G++ +YFI L + NY LLN+FLAIAVD+L A+ +T+ Q EK++E EE+KE I A G PP + + E E + A E E L +A P+ S+ FIFS N FR H +V +F I+ I+ SSI+LAAEDPV S RN L YFD FT +F IE+ LK+ YG LH GS+ R +N++D+LVVS ++ SF + ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GK + C+D SK+ + +G Y Y + P I PR W KF +DNV AM+ LF V T EGWP +L +S+ + D+ PI +RVE+SIF+I+Y I+ FF +NIFV +I+TFQEQGE E + E+DKNQ+ C+++A+ A PL RY+P+ + +Y++W +V ST FEY + LI+LNT L M++Y Q + + ++ +N +FTG FTVE K LI++ P YF D WN FD + VVGSIVD + E + I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLI MLFFIYA+IGMQVFG I + TE NR+ NFQTF +++LFRCATGEAW IMLAC G C P + N+T G E CGS+F+ YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK ++QA+ ELR I K+W + +D +VPP G +TVGK Y LI E ++ K
Sbjct: 457 KQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSM---------------PTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMS-PLGISCWRCVRLLRIFKITRYWNSLSNLVASLLNSLRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFISPNYILLNLFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQ------SSAINVVKILRVLRVLRPLR-INRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAESKGNYITYKTGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLPRYIPKNQ--HQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILK-------------------------------------LIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVHPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-----ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEG--NLEQANEELRAIIKKIWKRTSMKLLDQVVPP---AGDDEVTVGKFYATFLIQEYFRKFK 1661 HSP 2 Score: 166.392 bits (420), Expect = 3.808e-40 Identity = 172/728 (23.63%), Postives = 298/728 (40.93%), Query Frame = 0
Query: 419 PYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLS-------TMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFF-YCTDD-------SKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFV-NIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINA---------------SPLERYMPE------------------ERVGFRY----------RLWR------------IVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFA-KNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNF 1057
P ++ + N R A ++V + F++ I+L I A+ +ALA P D N L +Y F +F +E LK+I YG++ HP +YLR WN++D ++V + S ++ A G N +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A+I ++LF GK C + ++ED C E + + W K ++DN AMLT+F T EGW VL Q +M E+ Y V ++F FFV N+ + ++ F ++ E E+ +++++ K +D+ A P MP E G R R WR V+S F + ++ L+ LNT + ++Y QP ++ N FT E K+ ++G++ +YF +N FD V G I++ ++VE + + + R R R+ K+ R ++ L+ + + S +++ ++LL+ + I++++GMQ+FG +F+ D R F F ++ +F+ TGE W S+M A G C YF+ ++++LNLF+A+ +DN
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNG--TVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQ-------------------------------------AYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISCWRCVRLLRIFKITRYWNSLSNLVASLLNSLRSIASLLLLLFLFIIIFSLLGMQLFGG-KFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYD------------GIMAYGGPSFPGMLVC------IYFIILFISPNYILLNLFLAIAVDNL 786 HSP 3 Score: 99.3673 bits (246), Expect = 1.385e-19 Identity = 153/678 (22.57%), Postives = 281/678 (41.45%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHY-----NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGL----RIYFESSFNRFDCLVISGSVFEVVWTH---------FKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLF-----------GGTFNFPDGTPPS------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSL---YFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMT-------ALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEE----EEELANQAPPVLPYSSMFIFSST----NSF-RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAI--TSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALII 715
+ILL + C VA+ Y + + + L E++FL +F E FL++ A GL Y + +N D +++ +F + K F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + P PS NF++F+ AMLTVFQ +T E W +V+Y ++ G Y L YF+ L +FG++ +LN+ L + + +E L+EK++ +E+ K E ++ ++ + + ++ E + E N + S FS N F R V +F ++ ++ +++ +A+E N + + +F E+LLK+ G+ +Y L+N D +V I T + S G + R +R+LR K + L + ++NSL+++ ++L++ LF IF+++ +QLF GKF + D+ Q RR F DN P ++LT+F + T E W +V+ + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 159 IILLTIFANC-VALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKIMSPLG-----ISCWRCVRLLRIFKITRYWNSLSNLVASLLNSLRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF---------DEMQT--------------------RRSTF--DNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFISPNYILLNLFLAIAV 783
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|32172454|sp|Q24270.2|CAC1D_DROME (RecName: Full=Voltage-dependent calcium channel type D subunit alpha-1; Short=DmCa1D) HSP 1 Score: 922.924 bits (2384), Expect = 0.000e+0 Identity = 542/1267 (42.78%), Postives = 725/1267 (57.22%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPS---NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQT-GLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKER----IEYERRKAEDAAAAAARALTAE-------------------------EEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF------------------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGS--TCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILE 1209
K+ + GY WI +AE+ I L K + + STE+ EE E ++ + R K KK DF ++ R RK VK Q FYW +I+LV LNT +A EHY Q WL F T F+GLF EM L+M++LG + YF S FNRFDC V+ GS+ E + T+ G+SVLR +RLLR+FKVT YW SL NLV SLL+S++SI SLLLLLFLFI+IFALLGMQ+FGG FNF DG NF+ F A+LTVFQI+TGEDWN VMY GI + GG + G + +YFI L + GNY LLNVFLAIAVD+L A +L E EKE+E E+A + ++ P P + I+ E+++ +D L+ E E A + P+ P +S F+FS TN FR H + F I+ I+ SS LAAE+P+ + NK L FDY FT VF IE++LK+I YG +LH G++ R +N++D+LVV C + + +S +K LRVLRVLRPL+ I R LK V CVI ++K + NI++V L F+FAVI VQLF GKFF CTD SK D+C G Y +Y D H P + R W +FH+D+V MLTLF V T EGWP +L S+ + + PI FR ++ +YI+Y I+ FF VNIFV +I+TFQ +GE E + ++DKNQ++CI+FA+ A P+ RY+P+ G +Y++W V S+ FEY I LI++NT L MK+Y QP+ + L +N +FT F +E FK+ A K +YF D WN+FDF+ V+GS +D + E N I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLI +LFFIYA++GMQVFG I D + NFQTF ++VLFR ATGEAW IM++C + CD N+ + P + CGS+ +Y YF+SF LCSFL++NLFVAVIMDNFDYLTRD SILG HHLDEFIR+W+EYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+++R +LSIK +D A+ ELR TI ++W + +D +VPP +TVGK Y LI +
Sbjct: 900 KQQIEEDLRGYLDWITQAED---------------IEPDAVGGLISDGKGKQPNEMDSTENLGEEMPEVQMTESRWR--------KMKK--DFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTN-TGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNF-DGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADAD---SLSEVEKEEEPHDESAQKKSHSPTPTIDGMDDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKILPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVV-CVSLISLVSSSNA-----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKH--GIQYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFK-------------------------------------NYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSATGEAWQEIMMSCSAQPDVKCD-----MNSDT-----PGEPCGSSIAYPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDG--NIDDANSELRATIKQIWKRTNPKLLDQVVPPPGN--DDEVTVGKFYATYLIQD 2077 HSP 2 Score: 191.815 bits (486), Expect = 7.212e-48 Identity = 186/744 (25.00%), Postives = 320/744 (43.01%), Query Frame = 0
Query: 416 PVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPV-NTDSP-RNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFF-YCTDDSKEDADD----------CQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITF-QEQGESE--------LEEAEIDKNQKSCIDFAINASPLE--------------------------RYMPE---------------ERVGFRYR--LWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFA-KNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGN-IRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIY 1091
P P ++F S N R +V + F+ I+L I A+ IALA P +DS N+ L +Y F +F E ++KI+ YG +LH G+YLR WN++D +V S A+ L ++ +K+LR RVLRPL+ + VP L+ V + ++ ++ +F+I ++ L I+A+I ++LF+GK C D+ + ++ C Y Y D I ++DN AMLT+F T EGW VL A +D +++I I+ FF +N+ + ++ F +E+ +++ E+ +I+++ + +D+ A +E MPE +RV R R + V+S F + I+ L+ LNT +L ++Y Q + Y N F G FT E K+ ++G + YF +N FD V+GSI + L+ + V LR R R+ K+ + ++ L+ + + S +++ ++LL+ + I+A++GMQVFG FD E R +NF F ++ +F+ TGE W ++M G Y A + YF+ +F+C ++++LN+F+A+ +DN + A L E + +D SA+ + +
Sbjct: 588 PDRPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQL----MKDAFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKACRDEITGEYEENIRPCGVGYQCPPGYKCYGGWDGPNDGIT---------NFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDW---------QWMYFISMVILGAFFVMNLILGVLSGEFSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQ-------------------------------------GYFVSLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYR-MNFDCFWQALLTVFQIMTGEDWNAVMYV---GINAYGGVSSYGA---------------LACIYFI-ILFICGNYILLNVFLAIAVDN---------LADADSLSEVEKEEEPHDESAQKKSH 1243 HSP 3 Score: 129.413 bits (324), Expect = 9.135e-29 Identity = 166/684 (24.27%), Postives = 298/684 (43.57%), Query Frame = 0
Query: 115 YWFVILLVLLNTCSVAVEHY-----NQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFD-CLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN---------------FPDGT----PP---------------SNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEE----------EELANQAPPVLPYSSMF-----IFSSTN-SFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE-SELEEAE 730
+ F+ILL + C +A+ Y + + ++ L E+VFL +F E +++ A G + Y + +N D +V+ G++ + K +F + LRA R+LR ++ SL+ ++ S+L +M + + LL+ I+I+A++G++LF G + P G PP +NF++F +AMLTVFQ +T E W +V+Y ++ G +YFI + + G + ++N+ L + E KE +AK R + + R K++IE + R +AED A L ++ + E L + P V S + F N R A V F I++++ ++ LA E D N + Y + F +F E+LLK+ G Y L+N D VV +IT + G +S ++ +R+LRV + K + + L A ++NS++++ ++L++ LF IFA++ +Q+F GKF + D KE+ + + ++D A+LT+F + T E W AV+ + A +S + I++I+ FI + +N+F+A+ + + SE+E+ E
Sbjct: 617 FEFLILLTIFANC-IALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKD---AFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKACRDEITGEYEENIRPCGVGYQCPPGYKCYGGWDGPNDGITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQW----MYFISMVILGAFFVMNLILGVLSG------------EFSKERNKAKN---RGDFQKL----REKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQ-EYTNVFFIGLFTCEMLLKMYSLGF----QGYFVSLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVA---SLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNF---DGKEE---------------------------KYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINA----YGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEE 1231
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|46576355|sp|O57483.1|CAC1S_LITCT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=FGalpha1S; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 922.154 bits (2382), Expect = 0.000e+0 Identity = 513/1163 (44.11%), Postives = 711/1163 (61.13%), Query Frame = 0
Query: 106 RKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPD-GTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQ-TGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAA------------------IRAAYGPIPPGPRTK-ERIEYERRKAEDAAAAA------------------ARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAI--APRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEF------------AKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
R VK ++FYW VI+++LLNT +A EH++QP L++ V L LF EM ++++ALG + YF S FNRFD V+ + EV+ G+SVLR +RLLRIFK+T YW+SL NLV SLL+S++SI SLLLLLFLF++IFALLGMQ+FGG F+F D S F++F A++TVFQILTGEDW VMY+GI + GG +G+ +YFI L + GNY LLNVFLAIAVD+L +A+ +T+ Q+ + E+ + K+ A +A IP R K + E + +D +A R L E +L +A P+ SS FIFS TN R H ++ F FI+L I+ SSI+LAAEDP+ +S RNK L+ D FT +F E++LK+ YG LH GS+ R +N++D+ VV ++ S ++ +S +K LRVLRVLRPL+ I R LK V C+ ++K + NI++V L F+F+ I VQLF GKF+ CTD +K AD+C+G +F+ A D +P + PR+W FH+DNV + M++LF + T EGWP +L +++ + D PI +R+E+++F+IVY I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+ +A+ A PL RY+P+ +Y++W +V S+ FEY + LI LNT L M++Y Q S YM +DIL N+ FT F++E LKL ++ YF D WN+FDF+ V+GS++D ++ E + I++ F RLFR RLVKLL +G +R LLWTF++SF+ALPYV LLI MLFFIYA+IGMQVFG I T NR+ NFQTF +++LFRCATGEAW I+LAC G CDP + + G+ E TCG++F+Y YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIWAEYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLFSL+R L IK +Q++ ELR I K+W + +D ++PP IG +TVGK Y LI E+++ K
Sbjct: 425 RDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDSLTKAQDIANEVLLALFTMEMIVKIYALGFQSYFMSLFNRFDSFVVCTGLLEVMLVA-SDIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDLEVRRSTFDTFPQALITVFQILTGEDWTAVMYNGIMAYGGPTYSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLARANPDKTEEEKLLLAKKEQKAKGEGIPTTARLKIDEFESNVNEIKDPYPSADFPGDDEEEEPEIPISPRPRPLA---ELQLKEKAVPMPEASSFFIFSPTNKIRVLCHRIINATTFTNFILLFILLSSISLAAEDPIQPESFRNKVLSKLDIVFTVIFTTEIVLKMTAYGAFLHKGSFCRNSFNILDLSVVGVSLISMGIE------SSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFV--AKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHAEDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVIVTFQEQGEQEYKDCELDKNQRQCVQYALKARPLRRYIPKN--PHQYKIWYVVTSSYFEYLMFFLITLNTISLGMQHYGQTAEFS----YM------------------------------SDIL---NVAFTGIFTVEMFLKLAAFKAKGYFGDPWNVFDFLIVIGSVIDVILSEIDTPGIPATPGAEESSRISITFFRLFRVLRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALVDGTHINRNSNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPMS---DFQPGE----EYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEG--NFEQSNEELRMIIKKIWKRTSMKLLDQVIPP---IGDDEVTVGKFYAIFLIQEHFRKFK 1524 HSP 2 Score: 185.652 bits (470), Expect = 4.310e-46 Identity = 175/707 (24.75%), Postives = 314/707 (44.41%), Query Frame = 0
Query: 422 SMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTD--SPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDM------LGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGK-----FFYCTD-----DSKEDA---DDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINASPLERYMPEERVGF---------------------------RYRLWR---------IVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFD-FVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYN---ATSGKILDPEKTCGSTFSYTYFVSFIFLC-SFLMLNLFVAVIMDNF 1057
++F N FR +V + F++ I+L I A+ +ALA P+ D + N L +Y F +F IE LKI+ YG ILH +YLR WN++D +VS + S ++ L + G + +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A++ ++LF+GK +F TD D+++ A QG + ++ P H+DN AMLT++ T EGW VL A ++ P I+++ ++ FF +N+ + ++ F ++ E E+ +D++ + +D+ +A ++ M E R GF ++RLW +V+S F + ++ +I+LNT ++ +++ QP ++ N V FT+E K+ A+G + SYF +N FD FV G + L+ + + + LR R R+ K+ R ++ L+ + + S +++ ++LL+ + I+A++GMQ+FG +FD + R F TF ++ +F+ TGE W ++M YN A G C YF+ +F+C ++++LN+F+A+ +DN
Sbjct: 30 ALFCLGLQNPFRKFCINIVEWKPFEMIILLTIFANCVALAIFLPMPEDDTNSTNSVLEKVEYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQCSINGSECRGWWPGP---NNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDA-IGNEWPW--------IYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQMLREQQAMDEDLRGYLDWITHAEVMDPDM-EPRDGFSQLEEGGSETDSLYEIEGINKFIAFFRQWRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDSLTKAQDIANEVLLALFTMEMIVKIYALGFQ-------------------------------------SYFMSLFNRFDSFVVCTGLLEVMLVASDIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGG-KFDFEDLEVRRSTFDTFPQALITVFQILTGEDWTAVM---------------YNGIMAYGGPTYSGMSVC------IYFI-ILFVCGNYILLNVFLAIAVDNL 663 HSP 3 Score: 117.857 bits (294), Expect = 2.514e-25 Identity = 158/698 (22.64%), Postives = 287/698 (41.12%), Query Frame = 0
Query: 118 VILLVLLNTCSVAVEHYNQP----HWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKP--------KAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN----FPDGT----------------------------------PPSN----FNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAED-AAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSF--------------RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITS---FAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQ---GESELEEAEIDKNQK 736
+ILL + C P + + L E++FL +F E FL++ A G + Y + +N D ++S VF V+ K+ F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + F D P+N F++F AMLTV+Q +T E W EV+Y ++ G +YF+ L L G++ +LN+ L + + E T KE E+AK R A+ + R ++ ++ + R D A E + + S++ N F R +V F ++++I+ +++ +A E DS K + +F +E+++KI G SY L+N D VV + A D++ G + LR +R+LR K + L + ++NS++++ ++L++ LF IFA++ +Q+F GKF D +D + RR F D P A++T+F + T E W AV+ + A + + + I++I+ F+ + +N+F+A+ + E ++ +AE K +K
Sbjct: 56 IILLTIFANCVALAIFLPMPEDDTNSTNSVLEKVEYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQCSINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPW----IYFVSLILLGSFFVLNLVLGVL------SGEFT------KEREKAKS---RGAFQML----REQQAMDEDLRGYLDWITHAEVMDPDMEPRDGFSQLEEGGSETDSLYEIEGINKFIAFFRQWRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDS-LTKAQDIANEVLLALFTMEMIVKIYALGF----QSYFMSLFNRFDSFVVCTGLLEVMLVASDIMSPLG------ISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKF---------DFEDLE--------------------VRRSTF--DTFPQALITVFQILTGEDWTAVMYNGIMAYGGP----TYSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKK 684 HSP 4 Score: 110.153 bits (274), Expect = 6.582e-23 Identity = 178/827 (21.52%), Postives = 330/827 (39.90%), Query Frame = 0
Query: 25 NEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEE------------------LLSKKKRAPG-LALKKK---FKKNSDFWVYE--KKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPH-WLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSF--NRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN-------------------FPDGTPPS-------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIY------SLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTY----FDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAG-----GENLSTMKSL---RVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFR--------VEMSIFY-IVYFIVFPFFFVNIFVALIIITFQE-------QGESELEE-----AEIDKNQKSCIDFAINASPLERYMP 753
N D+T EE+ ++ A+K K + + T + + E E E ++ +S + R L LK+K + S F+++ K R + + F F++L +LL++ S+A E QP + ++ L + VF +F E+ L+M A G ++ + SF N F+ L +S ++ + A S + +LR LR+LR + L+++V L ++K+I +++L+ L +F+ +G+QLF G F DG P +F++ M+++F I T E W +++Y I+S G IY +++FI + + ++N+F+ + + +E+ ++E K+ + ++ ++Y A AR L + N + I VVT F+ + +I ++I+L + T + +Y + FT +F +E+ LK+ + Y + WNV D L+V ++ + + + G G S+ S+ R+ RVLR +K + R ++ + I S + + + ++ ++ FI+AVI +Q+F G+ L T + + ++ P A+L LF T E W +L D P+S F+ + FY I ++++ F +N+FVA+I+ F G L+E AE D K I + L R P
Sbjct: 689 NPDKTEEEK---LLLAKKEQKAKGEGIPTTARLKIDEFESNVNEIKDPYPSADFPGDDEEEEPEIPISPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKIRVLCHRIINATTFTNFILLFILLSSISLAAEDPIQPESFRNKVLSKLDIVFTVIFTTEIVLKMTAYGAFLH-KGSFCRNSFNILDLSVVGVSLISMGIESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFVAKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSH-AEDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVIVTF-----------QEQGEQEYKDCELDK---------NQRQCVQY---------ALKARPLRR---------------------YIPKNPHQYKIWYVVTSSYFEYLMFFLITLNTISLGMQHYGQT-----AEFSYMSDILNVAFTGIFTVEMFLKLAAF----KAKGYFGDPWNVFDFLIVIGSVIDVILSEIDTPGIPATPGAEESSRISITFFRLFRVLRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-------------------GKIALVDGTHIN-----------RNSNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCD--PMSDFQPGEEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQP 1418
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|6165983|sp|O73700.1|CAC1D_CHICK (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=CHCACHA1D; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 918.302 bits (2372), Expect = 0.000e+0 Identity = 545/1292 (42.18%), Postives = 759/1292 (58.75%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEE--RRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEE----ELLSKKKRAPGLA-------LKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDG-TPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGG-AQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEE------------------------------------LANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPT----------SYFKDFWNIFDFVTVVGSIVDALMVEFAKN-----------------FINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATK 1215
K+ + +GY WI +AE++ D +EE R + A +K+ +L G+S + + E E E +S + P K KF + W + + R VK FYW VI+LV LNT +++ EHYNQP WL++ V L LF EM ++M++LGL+ YF S FNRFDC V+ G + E + + + G+SV R +RLLRIFKVT +W+SL NLV SLL+SMKSI SLLLLLFLFI+IF+LLGMQLFGG FNF + T S F++F A+LTVFQILTGEDWN VMY GI + GG + +G+I +YFI L + GNY LLNVFLAIAVD+L A+ + Q++E E++E K+ A + + + E+ E +++K +D+ A E+E++ + + P+ S+ FIFSSTN R H ++ +F I++ I+ SS++LAAEDP+ + S RN L Y DY FT +F E++LK+ +G LH GS+ R +N++D+LVV ++ SF + +S +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L F+FA I VQLF GKF+ CTD++K++ ++C+G Y +Y D P + R+W+ F++DNV +AM+ LF V T EGWPA+L +++ + + P+ +RVE+SIF+I+Y I+ FF +NIFV +I+TFQEQGE E + E+DKNQ+ C+++A+ A PL RY+P+ ++Y+ W +V ST FEY + LI+LNT L M++Y G L D + MN+ FT F++E VLKLI++ P YF D WN FD + V+GSIVD ++ E I++ F RLFR RLVKLL +G IR LLWTF++SF+ALPYV LLIAMLFFIYA+IGMQVFG + + + NR+ NFQTF +++LFRCATGEAW IMLAC G CDP + YN E TCGS F+ YF+SF LC+FL++NLFVAVIMDNFDYLTRD SILG HHLDEF RIW+EYDP A+GRI + ++ +L+ + PPLGFG CP R+A K+L+ MN P++ +G V F TLF+L+R L IK ++QA+ ELR I K+W + +D +VPP G +TVGK Y LI + ++ K
Sbjct: 423 KQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRK--CRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMS-PLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLEN-----KKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQ------SSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKN--PYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHY-------------------------------------GQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPES-DYNPGE------EYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEG--NLEQANEELRAVIKKIWKKTSMKLLDQVVPP---AGDDEVTVGKFYATFLIQDYFRKFK 1649 HSP 2 Score: 134.035 bits (336), Expect = 3.674e-30 Identity = 104/381 (27.30%), Postives = 179/381 (46.98%), Query Frame = 0
Query: 404 AEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPR--NKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDML--------GSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKF----FYCTDD--SKEDADDC----QGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESE---------LEEAEIDKNQKSCIDFAINASPLERYMPEE 755
++++ +N PP ++F S N R A ++V + FD+FI+L I A+ +ALA P D N L +Y F +F +E LKII YG++LHP +Y+R WN++D ++V + S ++ L S G +K+LR RVLRPL+ + VP L+ V + +I ++ + +I ++ L I+A+I ++LF GK F D +ED C G + + T+ + P ++DN AMLT+F T EGW VL A + I+++ I+ FF +N+ + ++ F ++ E E+ +++++ K +D+ A ++ EE
Sbjct: 86 SKKQGNTSNSRPP----RALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGP---NGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWP---------WIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEE 450 HSP 3 Score: 108.997 bits (271), Expect = 1.400e-22 Identity = 159/709 (22.43%), Postives = 290/709 (40.90%), Query Frame = 0
Query: 114 FYWFVILLVLLNTCSVAVE---HYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGL----RIYFESSFNRFDCLVISGSVFEVV----------WTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN----------------------------------------FPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMT-------ALQEKEK---------------------EDEEAKEAAIRAAYGPIP-PGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSF---------------RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTD------SPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALII 715
F F++L + N ++AV + + + L E+ FL +F E FL++ A GL Y + +N D +++ +F V+ +H K G F + LRA R+LR ++ SL+ ++ S++ +M ++ + LL+ I+I+A++G++LF G + P+G +NF++F+ AMLTVFQ +T E W +V+Y + G + I YF+ L + G++ +LN+ L + + +E L+EK++ DEEA E R + K R+ R + A+ + E ++ + S+ S + F R A+ +V + L V + L +++ +++E D NK L FTC E+L+K+ G+ +Y L+N D VV I + L +S + +R+LR+ K + L + ++NS+K++ ++L++ LF IF+++ +QLF GKF + D+ Q ++ +DN P A+LT+F + T E W AV+ + A P S + + I++I+ FI + +N+F+A+ +
Sbjct: 123 FDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGG-ITNFDNFAFAMLTVFQCITMEGWTDVLY-WVNDAIGCEWPWI---YFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFL----NTLTISSEHYNQPDWLTQIQDIANKVLLAL---FTC----EMLVKMYSLGL----QAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRI---FKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNF---------DETQ----------------------TKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAY---GGPSSSGMI-VCIYFIILFICGNYILLNVFLAIAV 773 HSP 4 Score: 74.7146 bits (182), Expect = 4.783e-12 Identity = 63/275 (22.91%), Postives = 110/275 (40.00%), Query Frame = 0
Query: 839 LSYMNLTFTLCFSIECVLKLISYG----PTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNF------------INVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVF---------------------------GNIR-------------FDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNF 1057
L + F + F++E LK+I+YG P +Y ++ WN+ DFV VV + ++ + K +V LR FR R ++L+ +++++L + +++ L ++ LL+ + IYAIIG+++F GN R P+ NF F ++ +F+C T E W ++ C+ + + YFVS I L SF +LNL + V+ F
Sbjct: 156 LEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGI---TNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCE-----------------------WPWIYFVSLIILGSFFVLNLVLGVLSGEF 404
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1325281706|ref|XP_023325541.1| (LOW QUALITY PROTEIN: voltage-dependent calcium channel type A subunit alpha-1-like, partial [Eurytemora affinis]) HSP 1 Score: 1582.39 bits (4096), Expect = 0.000e+0 Identity = 824/1215 (67.82%), Postives = 975/1215 (80.25%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSDFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKF 1216
++ + GY WI +AEEVIL E+RTTEEER +I+EAR+RA K+KKL+ LGK S+++E+E E+ + K A ++ + FW EK+ R+ IR VK+QWFYW VILLV LNTC VAVEHYNQP+WL++FL E+VFL LF+ EM +RM+ALG RIYFESSFNRFDC+VI SVFE+V+T + AG FGLSVLRALRLLRIFKVT YWSSLRNLV+SLLSSM+SI+SLL LLFLFILIFALLGMQLFGG FNF DGTP +NF+SF I++LTVFQ+LTGEDWNEV + + +G++YSLYFI LTLFGNYTLLNVFLAIAVD+L AQE+TA E + EDEE K P+ P EEE +N+ P+LPYSS+F+ SS N FR +H VVT+P FD FIM++I+ SSI+LAAEDPV+ S N+ L YFDY FTC+F IE+LLKI+D GV+LHPGSY R+ WN++D +VV CA+ +F GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNVFNIL+VY+LF FIFAVIAVQLFNGKFF+CTD SK A+ CQGEYF + +D PP + R WR+Q+FHYDNV AMLTLFAVQTTEGWPAVLQ SM ATF +Q P LFR EMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQK+CIDFAI+A PLERYMP+ER F+Y++WR+V STPFEYFIMTLIVLNT LLMMKYY+Q LHYMNT+ TG FT+EC K+ + G +YH +P +L D+L YMN+ FTL F++EC LKL ++G SYF+ WN FDF+TVVGSIVDALMVEFAKNF NVGFLRLFRAARL+KLLRQG TIRILLWTF+QS KALPYVILLIAMLFFIYAIIGMQVFG++R++PDT+YNRH NFQTF +G+ VLFRCATGEAWP+IML+C+ CD A + N T+G+ILDPEK CGST +Y YFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHL+E+IRIWAEYDPSA GRI+Y+EMYDMLKNMDPPLGFGSKCPDRLA+KKLIRMN P+D EG V FTTTLF+LIRENL+IKMR+AEEMDQAD+ELR+TI+KVWP+ +EKI+LLVP +EIG+G LTVGK+YGG+L+LENWK T+F
Sbjct: 317 QQQLERELNGYVGWICRAEEVILAEERTTEEERIHILEARRRAAAKKKKLKNLGKKNSSDTEEEVEDTGVPVRGK------AQTRRRTGCTGFWTTEKRIRYKIRGIVKQQWFYWAVILLVFLNTCCVAVEHYNQPYWLTQFLEIAEYVFLCLFLTEMSVRMYALGTRIYFESSFNRFDCVVICASVFEMVYTSLRTDAGQFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSILSLLFLLFLFILIFALLGMQLFGGAFNFSDGTPDANFDSFVISLLTVFQVLTGEDWNEVXLLLLHA-----SGMVYSLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTA-AENKVEDEEEK-------LRPVQP-----------------------------VEEEGSNR--PILPYSSLFVLSSDNPFRVGVHAVVTYPFFDTFIMIIIVGSSISLAAEDPVDERSASNQLLAYFDYIFTCIFAIEMLLKILDLGVVLHPGSYCRDFWNILDAIVVCCALVAFLFA--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYILFQFIFAVIAVQLFNGKFFFCTDISKHTAEHCQGEYFEF-GSDSEPPRVYKREWRQQQFHYDNVAVAMLTLFAVQTTEGWPAVLQHSMGATFENQGPDPLFRTEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIHAKPLERYMPKERNSFKYKIWRVVVSTPFEYFIMTLIVLNTLLLMMKYYKQTETYKTTLHYMNTILTGLFTLECLMKIFSFGFRYHNSPLVLIDVLVYMNIIFTLLFTLECALKLTAFGWRSYFRQSWNTFDFITVVGSIVDALMVEFAKNFFNVGFLRLFRAARLIKLLRQGXTIRILLWTFIQSLKALPYVILLIAMLFFIYAIIGMQVFGSLRYNPDTDYNRHNNFQTFFAGLQVLFRCATGEAWPNIMLSCQGERPCDYRAHRRNETTGEILDPEKRCGSTLTYAYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLEEYIRIWAEYDPSATGRIHYSEMYDMLKNMDPPLGFGSKCPDRLAYKKLIRMNMPLDGEGKVQFTTTLFALIRENLNIKMRSAEEMDQADIELRETILKVWPFTAREKINLLVPATQEIGRGTLTVGKMYGGILMLENWKQTQF 1478 HSP 2 Score: 126.716 bits (317), Expect = 1.557e-25 Identity = 215/1031 (20.85%), Postives = 403/1031 (39.09%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSV----LRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFN-----------------------------FPDGT---------------PPSN----FNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARAL----------------------------TAEEEEELANQAPPVLPYSSMFIFSSTNSF-------RCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGE-NLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFF----FVNIFVALIIITFQEQGESELEEAEIDKNQKSC-----IDFAINASPLERYMP----EERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVG----LHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMV--EFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLF-FIYAIIGMQVFGN--------------------IRFDPDTE----YNRHVNFQTF-----GSGIMVLFRCATGEAWPSIM 995
+ +L ++ +C V + P+ L+ L TE FLG+F E L++ ALG + Y + +N D +V+ ++ + L V LRA+R+LR K+ SL+ ++ S++ ++ ++ + LL+ I+IFA++G++ + G + P G P+N F++ + AMLTVFQ +T E W ++Y ++ G +YF+ L + G++ +LN+ L + ++ +E+E E + A ++ R ++++E E +AE+ A R +++ EEE+ + PV + + F R I +V F ++L++ ++ +A E N + L +Y F C+F E+ +++ G + Y +N D +V+ ++ L + G+ LS +++LR+LR+ K K L+ + +++S++++ ++L + LF IFA++ +QLF G F + D DA+ +D+ ++LT+F V T E W V + A+ + Y +YFI+ F +N+F+A+ + E E +++ ++ ++ + P+ Y FR + +V F+ FIM +IV ++ L + PV L Y + +FT F +E K++ +GV H P SY +DFWNI D + V ++V L +N + LR+ R R +K +++ ++ + V S K + + IL++ +LF FI+A+I +Q+F F D+E Y R Q F ++ LF T E WP+++
Sbjct: 16 YTVLTTIIASCVVMSLEEHLPNGDKTVLAVQLEATESYFLGIFCVESCLKILALGFVLHPGSYLRNIWNIMDFIVVMTGFITLLTPDTEGSKNEEQLPVDLRTLRAIRVLRPLKLVSGVPSLQVVLKSIIKALAPLMQIGLLVMFAIVIFAIIGLEFYSGALHKSCYSLSDLEEIVTEGSKMVPCYEGSSELAPSGAYTCDLSISQCLEKWEGPNNGITSFDNIAFAMLTVFQCITMEGWTSILYWTNDALGSH----FNWIYFVPLIIIGSFFMLNLVLGV----------LSGEFARERERVENRHAFLKL---------RRQQQLERELNGYVGWICRAEEVILAEERTTEEERIHILEARRRAAAKKKKLKNLGKKNSSDTEEEVEDTGVPVRGKAQTRRRTGCTGFWTTEKRIRYKIRGIVKQQWFYWAVILLVFLNTCCVAVEH-YNQPYWLTQFLEIAEYVFLCLFLTEMSVRMYALGTRI----YFESSFNRFDCVVICASVFEMVYTSLRTDAGQFGLSVLRALRLLRIF---KVTKYWSSLRNLVISLLSSMRSILSLLFLLFLFILIFALLGMQLFGGAFNF--SDGTPDAN------------------------------FDSFVISLLTVFQVLTGEDWNEVXLLLLHAS--------------GMVYSLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENKVEDEEEKLRPVQPVEEEGSNRPILPYSSLFVLSSDNPFRVGVHAVVTYPFFDTFIMIIIVGSSISLAA---EDPVDERSASNQLLAYFDYIFTCIFAIEMLLKILDLGVVLH---------------------------------PGSYCRDFWNILDAIVVCCALVAFLFAGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV-FNILIVYILFQFIFAVIAVQLFNGKFFFCTDISKHTAEHCQGEYFEFGSDSEPPRVYKREWRQQQFHYDNVAVAMLTLFAVQTTEGWPAVL 932 HSP 3 Score: 118.627 bits (296), Expect = 4.762e-23 Identity = 88/305 (28.85%), Postives = 147/305 (48.20%), Query Frame = 0
Query: 439 VVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCA-ITSFAMDMLGSAGGENLST-MKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRL----------WRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
++ +P F+ ++ IIAS + ++ E+ P + +L + F +F +E LKI+ G +LHPGSYLR +WN+MD +VV IT D GS E L +++LR +RVLRPLK + VP L+ V +I +L + I ++ + IFA+I ++ ++G K Y D +E + Y + + P+ A W +DN+ AMLT+F T EGW ++L + + A S F I+++ I+ FF +N+ + ++ F + E
Sbjct: 8 IIEWPPFEYTVLTTIIASCVVMSLEEHLPNGDKTVLAVQLEATESYFLGIFCVESCLKILALGFVLHPGSYLRNIWNIMDFIVVMTGFITLLTPDTEGSKNEEQLPVDLRTLRAIRVLRPLKLVSGVPSLQVVLKSIIKALAPLMQIGLLVMFAIVIFAIIGLEFYSGALHKSCYSLSDLEEIVTEGSKMVPCYEGSSELAPSGAYTCDLSISQCLEKWEGPNNGITSFDNIAFAMLTVFQCITMEGWTSILY------WTNDALGSHFNW---IYFVPLIIIGSFFMLNLVLGVLSGEFARERE 303
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|321454423|gb|EFX65595.1| (hypothetical protein DAPPUDRAFT_333049 [Daphnia pulex]) HSP 1 Score: 1575.84 bits (4079), Expect = 0.000e+0 Identity = 831/1228 (67.67%), Postives = 962/1228 (78.34%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNSD-FWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKED---------EEAKEAAIRAAYGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAP-PVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + GY +WI KAEEVIL E+RTTEEE+ +IMEAR+RA KRKKL+ LGKSRST++EDEE E+E RA L K K K FW EK+ RF IR TVK+QWFYWFVI+LV NT VAVEHYNQP WL+EFL+ EF FLGLF+ EM ++M+ALG RIYFESSFNRFDC+VISGS+FEV+W+ FK +GSFGLSVLRALRLLR+FKVT YWSSLRNLV+SLLSSM+SI+SLL LLFLFILIFALLGMQLFGG FNFP+GTPP+NFNSF IA+LTVFQILTGEDWNEVMY GIESQGG++ G+IYSLYFI L LFGNYTLLNVFLAIAVD+L AQE+TA +E+++E+ E+ EA + G P + + + K + A T + E+ P P+LPYSSMFI S TN R A H VV P FD FIM+VI SSIALAAEDPV S RN L YFDY FT VF +E++LK+ID G+ILH GSYLRE+WNVMD +VV CA SF DM+GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNVFNIL+VY+LFHFIFAV+AVQLFNGKFFYCTDDSK DDCQGEYF +S D PP + R W+ Q FHYDNV AMLTLFAVQT EGWP VLQ SMAAT D PI +R+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDFAI A PLERYMP++R F+Y++WR+V STPFEYFIMTLIVLNT LLMMKYY+Q V+ LH YMN FT FSIEC+LK+IS+G ++FKD WN FDFVTVVGSI+DAL+VEF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGN+ DPD+ NRH NFQ+F G+++LFRCATGEAWP+IML+C G CDP A K+ +G++++ +K CGS +Y YFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDP+A G+I YTEM+DMLKNMDPPLGFG+KCP RLA+KKLIRMN P+D +G V FT+TLF+LIRENLSIKMR A+EMD+A+ ELR TI K+WP + K+ +DL++PP E+ G+LTVGKIYGGLLILENWK T+FG+
Sbjct: 330 QQQLERELNGYVEWICKAEEVILAEERTTEEEKLHIMEARRRAAAKRKKLKHLGKSRSTDTEDEENEDEPDEGFS-RASYLKSKVKNKGACKRFWRAEKRLRFKIRHTVKQQWFYWFVIVLVFFNTVCVAVEHYNQPPWLTEFLYYAEFAFLGLFMTEMCIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSAFK--SGSFGLSVLRALRLLRVFKVTKYWSSLRNLVISLLSSMRSILSLLFLLFLFILIFALLGMQLFGGAFNFPEGTPPANFNSFPIALLTVFQILTGEDWNEVMYQGIESQGGSRRGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQEEEDKEKQQQELEKEMEALHLGSEGS--PKLDSTSPSKKGKGKRGHKSEGNGDAKTGDNVEDDDIMGPKPMLPYSSMFILSPTNPVRRAAHWVVNLPYFDFFIMVVISLSSIALAAEDPVEEGSFRNDILNYFDYAFTGVFTVEMVLKVIDSGIILHQGSYLREIWNVMDAVVVICAAVSFTFDMMGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYMLFHFIFAVVAVQLFNGKFFYCTDDSKHTQDDCQGEYFTFS-YDKRPPEVKRREWKSQLFHYDNVMAAMLTLFAVQTGEGWPQVLQNSMAATQEDHGPILHYRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFAIQAKPLERYMPKDRESFKYKIWRVVVSTPFEYFIMTLIVLNTLLLMMKYYKQSVLYKETLH-------------------------------------YMNTAFTALFSIECMLKIISFGVRNFFKDPWNTFDFVTVVGSIIDALVVEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNLHLDPDSSVNRHNNFQSFIGGLLLLFRCATGEAWPNIMLSCIRGRQCDPRALKFE--NGQVIE-DKECGSNLAYAYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPNATGKILYTEMFDMLKNMDPPLGFGNKCPYRLAYKKLIRMNMPVDVDGKVQFTSTLFALIRENLSIKMRPADEMDEANQELRVTIKKIWPLQAKKVLDLIIPPDNELNTGKLTVGKIYGGLLILENWKTTRFGQ 1511 HSP 2 Score: 125.946 bits (315), Expect = 2.643e-25 Identity = 92/322 (28.57%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADD--CQGEYFLYSATDDHPPAIAPRL------------WRRQKF---HYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI + N R ++ +P F+ ++L IIA+ + LA E+ P +P +KL + F +F +E LKI+ G LH GSYLR +WN+MD +VV +M + A E ++ LR RVLRPLK + R+P L+ V +I ++ + I ++ L IFA+I ++ ++G + T S ED ++ +GE TD+ A+A W F +DN+ AMLT+F T EGW ++L + A + ++++ ++ FF +N+ + ++ F ++ E
Sbjct: 11 TSLFILTEKNIIRRYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTPLARKLEKTEPYFLGIFCVESTLKILALGFALHRGSYLRNIWNMMDFVVVVTG----SMTIFAEANVE--VDLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGA-LHKTCYSIEDLNEILAEGELATPCNTDNATQAVAGSYICDYNSSLCLEKWEGPNFGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSSYN---------WVYFVPLIVIGSFFMLNLVLGVLSGEFAKERE 316
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662932|ref|XP_019889118.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X13 [Ooceraea biroi]) HSP 1 Score: 1571.6 bits (4068), Expect = 0.000e+0 Identity = 812/1235 (65.75%), Postives = 952/1235 (77.09%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGPRTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R D + ++E+ P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFR---------DGRGETQTSEERRQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.946 bits (315), Expect = 3.532e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.736e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIKR---DMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662918|ref|XP_019889111.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X7 [Ooceraea biroi]) HSP 1 Score: 1570.06 bits (4064), Expect = 0.000e+0 Identity = 817/1249 (65.41%), Postives = 957/1249 (76.62%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGRNPLSEAQ 1225
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+ +E Q
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQLKPAEQQ 1678 HSP 2 Score: 125.176 bits (313), Expect = 5.119e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.708e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662916|ref|XP_019889110.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X6 [Ooceraea biroi]) HSP 1 Score: 1570.06 bits (4064), Expect = 0.000e+0 Identity = 817/1249 (65.41%), Postives = 957/1249 (76.62%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGRNPLSEAQ 1225
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+ +E Q
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQLKPAEQQ 1678 HSP 2 Score: 125.176 bits (313), Expect = 5.129e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.712e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662914|ref|XP_019889109.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X5 [Ooceraea biroi]) HSP 1 Score: 1568.52 bits (4060), Expect = 0.000e+0 Identity = 815/1242 (65.62%), Postives = 954/1242 (76.81%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.176 bits (313), Expect = 5.543e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 82.8037 bits (203), Expect = 4.146e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662910|ref|XP_019889107.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X3 [Ooceraea biroi]) HSP 1 Score: 1568.52 bits (4060), Expect = 0.000e+0 Identity = 815/1242 (65.62%), Postives = 954/1242 (76.81%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.176 bits (313), Expect = 5.554e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 82.8037 bits (203), Expect = 4.151e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662926|ref|XP_019889115.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X10 [Ooceraea biroi]) HSP 1 Score: 1568.13 bits (4059), Expect = 0.000e+0 Identity = 815/1242 (65.62%), Postives = 954/1242 (76.81%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAAYGP--IPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.176 bits (313), Expect = 5.480e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.539e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662930|ref|XP_019889117.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X12 [Ooceraea biroi]) HSP 1 Score: 1567.75 bits (4058), Expect = 0.000e+0 Identity = 815/1242 (65.62%), Postives = 954/1242 (76.81%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI D DT N+H NFQ+F G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDADTSINKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.176 bits (313), Expect = 4.932e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.164e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1134662912|ref|XP_019889108.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X4 [Ooceraea biroi]) HSP 1 Score: 1562.74 bits (4045), Expect = 0.000e+0 Identity = 812/1242 (65.38%), Postives = 954/1242 (76.81%), Query Frame = 0
Query: 2 KENFSKAFEGYFQWIIKAEEVILNEDRTTEEERRYIMEARKRALQKRKKLRTLGKSRSTESEDEEEEEEELLSKKKRAPGLALKKKFKKNS---DFWVYEKKARFWIRKTVKKQWFYWFVILLVLLNTCSVAVEHYNQPHWLSEFLHTTEFVFLGLFIFEMFLRMWALGLRIYFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTA-------------LQEKEKEDEEAKEAAIRAA--YGPIPPGP-------RTKERIEYERRKAEDAAAAAARALTAEEEEELANQAPPVLPYSSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAEDPVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMDMLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNGKFFYCTDDSKEDADDCQGEYFLYSATDDHPPAIAPRLWRRQKFHYDNVPTAMLTLFAVQTTEGWPAVLQQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKNQKSCIDFAINASPLERYMPEERVGFRYRLWRIVESTPFEYFIMTLIVLNTFLLMMKYYQQPVMMSVGLHYMNTVFTGCFTVECFFKVIAMGVKYHGAPPLLTDILSYMNLTFTLCFSIECVLKLISYGPTSYFKDFWNIFDFVTVVGSIVDALMVEFAKNFINVGFLRLFRAARLVKLLRQGYTIRILLWTFVQSFKALPYVILLIAMLFFIYAIIGMQVFGNIRFDPDTEYNRHVNFQTFGSGIMVLFRCATGEAWPSIMLACESGSTCDPAAFKYNATSGKILDPEKTCGSTFSYTYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFIRIWAEYDPSAEGRIYYTEMYDMLKNMDPPLGFGSKCPDRLAFKKLIRMNQPIDDEGMVHFTTTLFSLIRENLSIKMRAAEEMDQADLELRQTIIKVWPYEGKEKIDLLVPPREEIGKGRLTVGKIYGGLLILENWKATKFGR 1218
++ + Y WI KAEEVIL E+RTTEEE+ +I+E R+RA K+KKL+ LGKS+ST++E+EE EE++ + + K K KK FW EK+ RFW+R +VK Q FYWFVI+LV NT VAVEHY QP WL++FL EFVFL LF+ EMF++M+ALG R YFESSFNRFDC+VISGS+FEV+W+ K +GSFG SVLRALRLLRIFKVT YW SLRNLV+SLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF +GTPP+NFN+F IA+LTVFQILTGEDWNEVMY GIESQ A YSLYFI L LFGNYTLLNVFLAIAVD+L AQE++A LQE EKE E ++ A PP P R + + ERR+ ED P+LPYSSMFI S TN R A H +V FD FIM+VI SSIALAAEDPV+ D+PRNK L YFDY FT VF IE++LKIID G+ILHPGSYLRE WN+MD +VV CA+ SFA DM GS+ G+NLST+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNV NIL+VY+LF FIFAVIAVQLFNG+FF+CTD+SK + ++C+G +F++ P + R W Q FHYDNV AMLTLFAVQT EGWP +LQ SMAAT+ D+ PI FR+EMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDKNQKSCIDF I A PLERYMP+ER +Y++WRIV STPFEYFIM LIVLNT LLMMK++QQ L YMN FTG FT+EC K+ A GVKYH APP+L D L+ +NL TL F IEC+LKL ++G ++FKD WN FDF+TV+GSIVDAL++EF +NFINVGFLRLFRAARL+KLLRQGYTIRILLWTFVQSFKALPYV LLIAMLFFIYAIIGMQVFGNI + +T + H NFQ+F +G+M+LFRCATGEAWP+IML+C G CD A K + CGS +Y YFVSFIF CSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEF+RIWAEYDP+A G+I+YTEMYDMLKNMDPPLGFG+KCP+RLA+KKLIRMN P+D +G V+FTTTLF+LIRENLSIKMR A+EM+QA+ ELR TI +WP + K+ +DLL+P EE+ + +LTVGKIY LLILE+W++T+FG+
Sbjct: 464 QQQLERELNSYLNWICKAEEVILAEERTTEEEKMHILEGRRRAAAKKKKLKNLGKSKSTDTEEEEGEEDQDDGFSRAS---YFKSKVKKQGTCLQFWRAEKRFRFWVRNSVKSQQFYWFVIILVFFNTICVAVEHYGQPAWLTDFLFYAEFVFLALFMMEMFIKMYALGPRTYFESSFNRFDCIVISGSIFEVIWSALK--SGSFGFSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFSEGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQNMA-----YSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEEKEEDKEKQLQELEKEIESLQKPGDGGAPKVEICPPSPTQNFRDGRGETQTSEERRQDEDDDTGPK----------------PMLPYSSMFILSPTNPVRRAAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEDAPRNKILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSFAFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGRFFFCTDESKYEPEECKGSFFVFEEGSSLP-ELKERKWDLQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHQQTEQYKNTLKYMNMCFTGMFTIECILKIAAFGVKYHDAPPVLLDFLTVVNLVVTLLFLIECILKLAAFGCKNFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNISNNAETAIDEHNNFQSFFAGLMLLFRCATGEAWPNIMLSCTKGKVCDEKA-------NKTAEDANDCGSNLAYVYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPVDGDGKVNFTTTLFALIRENLSIKMRCADEMNQANDELRDTIRSIWPLQAKKMLDLLIPRNEELSRDKLTVGKIYVCLLILESWRSTRFGQ 1671 HSP 2 Score: 125.561 bits (314), Expect = 4.728e-25 Identity = 94/330 (28.48%), Postives = 160/330 (48.48%), Query Frame = 0
Query: 421 SSMFIFSSTNSFRCAIHTVVTFPLFDVFIMLVIIASSIALAAED--PVNTDSPRNKKLTYFDYGFTCVFGIEVLLKIIDYGVILHPGSYLRELWNVMD-MLVVSCAITSFAMDMLGSAGGENLSTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYLLFHFIFAVIAVQLFNG---KFFYCTDD------SKEDADDCQGEYFLYSATDDHP---------PAIAPRLWRRQK---FHYDNVPTAMLTLFAVQTTEGWPAVL---QQSMAATFADQAPISLFRVEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGE 723
+S+FI S N R ++ +P F+ ++L IIA+ + LA E+ P + +KL + F +F +E LKI+ G +LH GSYLR +WN+MD +VV+ IT+F S G + +++LR +RVLRPLK + +P L+ V +I ++ + I ++ L IFA+I ++ ++G K Y +D E C ++ D+HP +I W +DN+ AMLT+F T EGW A+L ++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 144 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAF------SQGIKRDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNA-----NSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 450 HSP 3 Score: 83.1889 bits (204), Expect = 3.847e-12 Identity = 87/355 (24.51%), Postives = 151/355 (42.54%), Query Frame = 0
Query: 117 FVILLVLLNTCSVAVEHYNQPHW----LSEFLHTTEFVFLGLFIFEMFLRMWALGLRI----YFESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGLSVLRALRLLRIFKVTVYWSSLRNLVVSLLSSMKSIISLLLLLFLFILIFALLGMQLFGGT-----FNFPD-------GTPPS------------------------------------NFNSFSIAMLTVFQILTGEDWNEVMYHGIESQGGAQTGLIYSLYFIFLTLFGNYTLLNVFLAIAVDSLGQAQEMTALQEKEKEDEEAKEAAIRAAYGPIPPGPRTKERIEYERR-------KAEDAAAAAARALTAEEEE 408
+ +LL ++ C V + PH L++ L TE FLG+F E L++ ALG + Y + +N D V+ + K L LRA+R+LR K+ SL+ ++ S++ +M ++ + LL+ I+IFA++G++ + GT +N D G PS +F++ AMLTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + ++ KE+E E +++ ++ R ++++E E KAE+ A R T EEE+
Sbjct: 170 YAVLLTIIANCVVLALEEHLPHQDKTILAQKLEATEVYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGIITAFSQGIK---RDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIEDINLIVREGELPSPCNANSDDEHPFGAHVCNANTSICLERWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNW----IYFIPLIVLGSFFMLNLVLGV----------LSGEFAKEREKVENRQSFLKL---------RRQQQLERELNSYLNWICKAEEVILAEER--TTEEEK 496 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 18
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BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1088_size63410:32069..44621- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1088_size63410-snap-gene-0.21 ID=maker-scaffold1088_size63410-snap-gene-0.21|Name=hypothetical protein DAPPUDRAFT_333049|organism=Tigriopus kingsejongensis|type=gene|length=12553bp|location=Sequence derived from alignment at scaffold1088_size63410:32069..44621- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_333049' has the following synonyms
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