BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007725 (supercontig:LSalAtl2s:LSalAtl2s44:25674:28922:-1 gene:EMLSAG00000007725 transcript:EMLSAT00000007725 description:"maker-LSalAtl2s44-augustus-gene-0.13")

HSP 1 Score: 407.912 bits (1047), Expect = 7.953e-122
Identity = 253/731 (34.61%), Postives = 398/731 (54.45%), Query Frame = 0

Query:   16 SDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDL--SSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVES--SDLPSKPVQKREAP---SPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDP---------SVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNS-TGHIVGAKATFIRWFGRANISEIQNE--KGRNGMDQQPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPN--------KLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGT-LYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKF 717
            ++ I +GLE YF  HG+++  HP   I      +     G   + +E+  +KLW    +    N+++L E FP D   ++VI+E  +N+L P  I  M+Q+ K + +++T+ G      C K+PVV   +  S +  K  ++R A    + + E +FF      SD F            FD          SV  YPE YC IV++M   CFE S+LE++   G++    E  + +L   +++  +N   +SGIFL +++F+  LGGI R+  T  IVGAKAT + WF + NI+E +    +G+  +    VD  T+EFE ++L I L+D  G PEGLK   +V RS+ DI+   I  DV  L  GY +++ YV  MLG+F+ V  R +L   GI S    I+ S+G+CS MG  + PMHS++P LLLG+G+DDMF+I+Q +N     +KA   +    ++ G  ++HAGV IT+TS+TDIV F +GGT+         LP+LRS+ +++A GIAA Y  QVTFFVAW+  D+KRI   R+G CC  +   + K N        ++   S+M + F      L +  VK  ++L T+ F +LG +G  L+E +F  + F+P  + +  WF+ +T +F   G     +Y  E+DL  +L +++ ++    +++DII S +SW   +  Y++            H ++E    E++TQFL SP+G Q+   F
Sbjct:   49 TNCIVRGLESYFQWHGRFIGKHPVFVICLCFAFLLASFAGFPYYRKEERRIKLWSSDKTPSRLNHEFLHEYFPSDFRFANVIVE-ADNVLDPAAIXAMFQMRKKISEIKTNYGDTLRTKCAKMPVVKTPNFLSFIFGKRRKRRVADDFFTSDYESNFF------SDSFEHEMEEEEDNSGFDKDDISYPERLSVEAYPEPYCNIVDSMEETCFESSVLELWGDNGEY-ESTEKRLESLSLIDILDTINNKNRSGIFLIKKNFTHMLGGITRDPITKEIVGAKATRMFWFLKMNIAEARKSPVEGQGVI----VDKKTMEFEKEMLDILLNDT-GLPEGLKISPHVSRSFFDIAGPTILGDVKILGIGYSIVFFYVLLMLGKFDWVGXRXYLAIAGIISVVFGIVFSIGICSAMGFFWSPMHSILPFLLLGIGIDDMFIIIQCWNTLNPYEKASSSV----DKFGYTMRHAGVAITVTSLTDIVAFGIGGTSX--------LPALRSFCIFSAVGIAAIYYFQVTFFVAWMVFDEKRISSGRNG-CCPCIEVKKVKKNTEEIMEDMEVESLSIMSKIFRFYASHLKFKPVKFLIILITMGFTSLGAYGNYLIEQKFDPIWFIPPHTYLSDWFEANTKWFSDKGGFFVNVYVYEVDLSVDLMELERIIRAIESQSDIIDSVNSWTTNYIKYVD-----------THGLAEGTLYERITQFLHSPMGLQYKRNF 742          

HSP 2 Score: 210.305 bits (534), Expect = 1.730e-55
Identity = 106/234 (45.30%), Postives = 155/234 (66.24%), Query Frame = 0

Query:  845 STQHIPAMNWVKDSVVDAGF--PGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQS-GSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNEKPPSYVIAHDAKMEINHNEEQDLVD 1075
            S   I AMN +KD +  A     G  F ++Q YS WE DE+I  EL++N+  A + V I T ++I+N  +  +V+  V+++++DV G M+FWGLTID V+   +IIS+GLCVD+S HVAH F++++ GS+++R+   L  IGPAV NGGIST +A  +L  S SHVFI++FKVFFL++ FGLFHGL+FLPV+LSL+G  P      + ++  S +I+ + +  IN      L D
Sbjct:  781 SKIKISAMNRIKDILNSANLTSSGRIFPLSQGYSMWETDEIIGYELYKNIIFAFVAVFIMTWIVISNFVASFLVLFAVMLTVLDVSGIMHFWGLTIDTVSCNFLIISIGLCVDYSTHVAHRFLIEAEGSRDQRIVKALTNIGPAVMNGGISTFLAFCLLCGSSSHVFITFFKVFFLVVTFGLFHGLVFLPVILSLIG--PISYGSDTVDRSQSVIISDNKQDSINLVSPSKLKD 1012          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010036 (supercontig:LSalAtl2s:LSalAtl2s660:189355:234598:1 gene:EMLSAG00000010036 transcript:EMLSAT00000010036 description:"maker-LSalAtl2s660-augustus-gene-1.6")

HSP 1 Score: 229.18 bits (583), Expect = 2.785e-65
Identity = 149/446 (33.41%), Postives = 233/446 (52.24%), Query Frame = 0

Query:   62 EKNMVKLWIPPNSDFAHNNQWLWENFPPDLSSVI---LEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDV--ESSDLPSKPVQKREAPSPNTEEDFFGDFG----------DESDDFAAIKSGAGVGKAFDPSV-VFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQQPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNN----SEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTT 486
            E  M KLWIP NS+F  N +W+ ENFP +    I   L    NIL PE +  +  IH+ V Q+       W DLC+K+PV +V  E + + +     +       ++ F  D            D  D F   ++ +  G  ++  + +  P+ YC  VE +   CFE S+LE++    +F  +    I  L ++ ++ A+N I  S ++  + ++  Y+G I RN TGH+V A      WF + N+   +N     G+  + V+  +LE+E + + I L+      E + +     RS+ DI+  +I+ D I LT G+F++ +Y   MLGR N VE R F    G+ S  L ++VS+G+ S MGL Y P+ +++P L LG+G+DDMF+IVQ + N    ++ E      IK L E + + +K AGV ITITS+TDI  F +GG T
Sbjct:   24 EYKMEKLWIPQNSEFISNKKWIEENFPTNYRVQIISLLSLSGNILTPEALLLLLDIHEKVEQISAGHNTRWKDLCFKVPVANVFLERNKVLT---NDKNTTFDRVDDLFISDDNVVPQNEKTEIDYDDAFLEYENESKNGSRYEKLLKILSPKVYCDFVETLNDRCFERSLLEIW----KFDRR---RIKRLTKDRIVQAINRIQNSPVYGYKTNYDNYMGSIIRNETGHVVSASTLNSVWFTQVNL---ENVSEVIGLTDEFVNKESLEWELKFVDIFLN---ASTENISAFGISARSFNDITKKSIFFDGIRLTGGFFIMCIYTVIMLGRLNCVEVRFFATIAGLFSILLGMVVSLGIISAMGLKYSPVFAMLPFLYLGIGIDDMFIIVQCWYNILEQNKKECSTDQSIKTLEEMMAETMKDAGVTITITSLTDIFAFGIGGIT 453          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000003602 (supercontig:LSalAtl2s:LSalAtl2s197:152115:159124:1 gene:EMLSAG00000003602 transcript:EMLSAT00000003602 description:"maker-LSalAtl2s197-snap-gene-1.29")

HSP 1 Score: 202.216 bits (513), Expect = 1.965e-53
Identity = 182/742 (24.53%), Postives = 327/742 (44.07%), Query Frame = 0

Query:   17 DAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCG-LGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDLSS----------VILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLP-SKPVQKREAPSPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNT-IGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQQPVDVTTLEFEQLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEM-GTLYF-----SEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLF-SPIGAQHMNKFQFAEPIACGTDAPKVLVSTVV 738
            + ++ G+   FY++G  VA +  + I G  +G+ +   LG   +  E +  K+W+P +S +  N + +      DL+S          VI+E E NIL  + +  +Y++H +++ L+    + + DLC+K      E   +P S P                                              P + C  +      C   SI E++      + K  + I  L  E+++ A++  +  S +F   R F   +G    N  G + GAKA  + +  + N S+      +NG+   P+   +++ E +   S  D+          +++ R+  +  NG + +DV     GY +++VY+   LG  N +E + +L  +GI   G+    S+G+  L+ +   PM+S++P +LLG+G+DDMFVIVQ+  N +  D  +G  K +   IG  +K AG  IT+TS+TDI+ F +GG +         LP+LR++ LY++ GI   Y   +TFF+ W  ID +R++  RD     ++    W P   +++S  E  F   G  +     +   ++ T+     G +G TL+E +F    ++  +S +  +F+    +F   G + G  Y      S +++ K   ++KN   +F  E     S  +++  F   +N               +  F    LT FL+ S +      KF+ ++   CG     V +S  V
Sbjct:   14 ERVNHGINWIFYQYGMIVAKYSYVFI-GLSLGIFIASTLGFIDYVTESDPTKIWLPQDSYYVQNTKKI-----KDLNSDAVNAATVIQVIVESEVNILRAKELNAVYKLHSSIINLKAPSNETFKDLCFK------ERVTVPISTP----------------------------------------------PSEICRALANAEWLCSAKSIFEIWTSDE--SSKEASIIAQLTDEDILDALSQDLAISKLF--RRPFRPIIGSKMFNDNGTLKGAKALLLSYSIKTNSSD------KNGI---PIWEKSVK-EHVFNYSFGDESSNLSVTLFSSSIFRA--EFQNG-MENDVQLFIFGYLIVFVYIIFNLGEMNTLEHKFYLAVVGIACVGMGYASSVGIFQLLSVYATPMNSILPFMLLGIGIDDMFVIVQASKNVKQNDDEEGENK-MDSYIGNIMKSAGXAITVTSLTDILAFGIGGFSQ--------LPALRNFCLYSSAGIFFVYFYIMTFFLGWFVIDARRVESHRDACLPCIIKKSSWVPC-YTRKSFAEXLFSCSGVLIDSLFFRGLAIIVTIVVSGFGVYGLTLIEPKFDPKSYINPESKLLSYFKASEQHFQKLGGIPGQFYILNPNNSLVEIEKVYEELKN--SEFIEEN----SLRTFHKAFSKTINS--------------TTNFQYSSLTNFLYTSRVPFDWAVKFKASQKPVCGDTTEDVKLSAFV 650          

HSP 2 Score: 164.466 bits (415), Expect = 2.869e-41
Identity = 80/186 (43.01%), Postives = 131/186 (70.43%), Query Frame = 0

Query:  866 GITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQS-GSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNE 1050
            G+ F  A  YS     ++I  EL RN+ LA++ V I  + L+A   + I V+L +++S++DV G MYFWG++ID  ++  + +S+GL VD+SAH+AH ++  S  S+ E+++ +L K+GPAV +GGIST +A ++L NS S VF ++FK+FFL+++FGL+HGL+FLPV+LS +G    ++ D++ +
Sbjct:  684 GLIFPHATQYSNLITLDIILSELTRNILLALMTVFIAALFLLAXALTSIYVLLSILLSIIDVTGYMYFWGVSIDTASAVLLTVSLGLAVDYSAHIAHGYIHSSKSSRGEKVKDSLTKVGPAVLSGGISTFLAFILLANSSSIVFTTFFKIFFLVVLFGLYHGLIFLPVLLSFIGPVYRQVQDSTKK 869          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007475 (supercontig:LSalAtl2s:LSalAtl2s429:50360:104533:1 gene:EMLSAG00000007475 transcript:EMLSAT00000007475 description:"maker-LSalAtl2s429-augustus-gene-1.9")

HSP 1 Score: 137.887 bits (346), Expect = 5.323e-35
Identity = 60/139 (43.17%), Postives = 99/139 (71.22%), Query Frame = 0

Query:  875 YSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFK 1013
            YS WE +E+I  EL+RN+ +A I + +   L +AN     +V+  V++++VD+ G ++FWG+TID +A  NI++S+GLCVD+S H+AH+F+  SG   ER  S++  IG A+ NGG +T +A+++L+ S+SH+FI +F+
Sbjct:  247 YSIWETEEIIVFELWRNMGIACIVIFVILSLFLANLLVSFLVLCNVIITVVDIVGFLHFWGITIDTIACVNIVLSIGLCVDYSIHIAHFFLCASGDAAERAASSVESIGIAIINGGTTTFMALILLIFSESHIFIYFFQ 385          

HSP 2 Score: 104.375 bits (259), Expect = 1.086e-23
Identity = 74/260 (28.46%), Postives = 127/260 (48.85%), Query Frame = 0

Query:  495 VLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDG--ACCWVVHGDQWKPN-KLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNE----TDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEF------FNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTVVDVE 741
            +LP LR +S+  A GIAA ++ Q+++F AW  +DQKRI + + G   CC        + N K  + S   +   A  R + +   ++ V++ +L  F+ G WG   +  EF   +F+P  S   QWF++   +    G    +Y   +D   ++PK+ N VD+F  +       I + + W+ +F+ Y     +  D+ +  H  S  F      F   L+ FL+S IGAQ+   F+F  P+ C   AP + + ++ + E
Sbjct:    1 ILPGLRHFSITCAIGIAAVFLCQISWFSAWFYLDQKRILNNKSGFFPCCIQYEDTSERENGKCFEFSFGNKLIRAFSRTIMHRTFQVIVIIISLILFSFGVWGAFSIRQEFDIKKFMPSSSYFVQWFEIKEKHSVSKGWQSNIYLGRIDPPLDIPKL-NSVDEFLQDLLDSKAYIITKEVWWTDFKLY-----ILRDKNV-SHWRSTLFKNDSFLFRVHLSDFLYSSIGAQYKVNFRFDTPLRCNQPAPNINIYSIWETE 253          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000005221 (supercontig:LSalAtl2s:LSalAtl2s276:209809:212602:-1 gene:EMLSAG00000005221 transcript:EMLSAT00000005221 description:"maker-LSalAtl2s276-augustus-gene-2.14")

HSP 1 Score: 135.191 bits (339), Expect = 1.500e-32
Identity = 90/305 (29.51%), Postives = 156/305 (51.15%), Query Frame = 0

Query:  361 CGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWV---VHG-----DQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTN 657
             G+ +I +Y   +   F+ V QR  L  +G    G+AI  S GL   +G+ Y  +H +IP LLLG+GVDD+F+I++S +N   ++K       L ERI   ++H+GV IT+TS+TD+V F +  TT+I        P LR++ L  A GI+  Y   VTFF+AW  +D++RI++ +  A  W    VH        W  N L ++  +E  F    + +   I        +L  F  G +GT  ++ ++  + ++  +S   ++ +     +P  G++  +Y   +  ++ + ++K  +D   N
Sbjct:   46 VGFLLILIYSLLVTSDFHPVNQRVMLLLLGFMVIGMAIGSSFGLGFHIGIIYADLHPIIPFLLLGIGVDDLFIIIKSTDNLSQKEKE----LPLKERIALVVQHSGVSITVTSLTDLVAFLISSTTSI--------PLLRAFCLSVALGISFLYFFVVTFFIAWYTLDERRIEEGK--AFLWFTKRVHSVPPKKSTWTHN-LYKKYYVEYLFAIPSQLILGGI--------SLLVFGFGIFGTFNIQQDYDPITYMRQNSYQVKFIEAKNRLYPEFGDIVDIYVGNIPYWENIQEMKQ-IDSLVN 326          

HSP 2 Score: 134.035 bits (336), Expect = 3.602e-32
Identity = 64/166 (38.55%), Postives = 111/166 (66.87%), Query Frame = 0

Query:  874 SYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQ-SGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            ++ +W  ++VIS+EL RN+ L   C++I   +L+AN    +IV+  V M++ ++ G  YF GL I+IV S  +I+SVGL +D+SAH+   + +Q +GS+ ER +  L  +GPA+F+GG ST +A ++L  + S++F S+  +    ++FGL++ L+F+P++LS  G
Sbjct:  447 AFHQWTANKVISKELIRNMSLNFACITIVLFMLLANIQLSLIVLFTVAMTITEIAGFAYFVGLNIEIVTSLELILSVGLVLDYSAHIGVCYTIQKTGSRRERSQRALLDMGPAIFSGGFSTFLAFVVLFFNDSYIFTSFCHMLGFGVLFGLWNALVFMPLLLSYFG 612          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010537 (supercontig:LSalAtl2s:LSalAtl2s6:1415448:1420769:-1 gene:EMLSAG00000010537 transcript:EMLSAT00000010537 description:"maker-LSalAtl2s6-augustus-gene-13.12")

HSP 1 Score: 107.071 bits (266), Expect = 2.172e-23
Identity = 60/178 (33.71%), Postives = 106/178 (59.55%), Query Frame = 0

Query:  865 PGITFAMAQSYSRWEIDE--VISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFML--QSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            P I+ ++   Y  + ID+   I+ +  + + +  + + I +  LI +      V   ++   + V G M +WG+ +D VA  N+I+ +G  VDFSAH+ ++++   +S S +ER+RS+L  +G  +  G  ST++ VL L  + S++F+++FK+ FL+IV G  HGL+ LPV+LSL G
Sbjct:  692 PDISISVFHPYFIY-IDQYLAIAPQTVQCILVTSLVMMIISFFLIPSLVCSFWVAFSIISIELGVIGYMTWWGVRLDGVALINLIMCIGFSVDFSAHICYHYITDTESSSPDERIRSSLYGLGLPIIQGATSTILGVLGLAFAPSYLFVTFFKMVFLVIVLGGLHGLIILPVLLSLFG 868          

HSP 2 Score: 98.2117 bits (243), Expect = 1.366e-20
Identity = 71/267 (26.59%), Postives = 111/267 (41.57%), Query Frame = 0

Query:  381 EQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACC-------------WVVHG---------DQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYF 625
            + +  +G  G+ S+ +A +   G    +G+++  ++   P LLLG+GVDD FVI+ S+  S            +PER+G+    A V IT+TS+TD   F  G  T          P +R + LYT   +A  Y   +T F A LA+  +   +   G  C             W+            D   P+     + M    D +GR L+    K  VLL   A+  + CWG T +E           DS   +++ +   YF
Sbjct:  298 KSKPLVGFAGLFSATIATIAGFGFACYVGVEFISLNFAAPFLLLGIGVDDTFVILSSWRRSSSN-------LSIPERMGRCYSEAAVSITVTSLTDFFSFMAGVVTP--------FPCVRIFCLYTGISVAFIYFWHLTLFGAILAVSGRAEKNNLHGMFCIKVIPKSLAVNKSWLTRTFMTGGTNPKDPHNPDDNKDHAGMVFFRDNMGRLLSRTWFKSLVLLLFGAYIVVACWGVTNIEEGLEKQNMANYDSYSVKYYSMDDSYF 549          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000008727 (supercontig:LSalAtl2s:LSalAtl2s542:206164:232722:1 gene:EMLSAG00000008727 transcript:EMLSAT00000008727 description:"maker-LSalAtl2s542-augustus-gene-2.8")

HSP 1 Score: 105.145 bits (261), Expect = 7.734e-23
Identity = 52/151 (34.44%), Postives = 92/151 (60.93%), Query Frame = 0

Query:  894 LAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFML--QSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPY 1042
            +A   +   +++ I N    + V   ++   V V G M  WG+ +D ++  N+I+ +G  VDFSAH+++++M   +S +  ER+R ++  +G  +  G IST++ V+ L  + S+VFI++FK+ FL+IV G  HGLL LP++LS+ G   +
Sbjct:  713 VAAFVMMFISLIFIPNPVCSLWVAFSIISIEVGVVGFMTLWGVGLDSISMINLIMCIGFSVDFSAHISYHYMSCEKSFTDKERIRDSMYALGLPIVQGAISTILGVVGLALAPSYVFITFFKMVFLVIVLGALHGLLVLPILLSIFGPSSF 863          

HSP 2 Score: 91.6633 bits (226), Expect = 1.233e-18
Identity = 86/367 (23.43%), Postives = 160/367 (43.60%), Query Frame = 0

Query:  359 LTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACC-------------WVVH---------GDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYF---PGDGEM---GTLYFSEMDLYKELPKIKNLVDKFTNET--DIIFSFDSWYPEFESYL--NDYFLQPDEAIPDHPIS 693
            L  G  + +  +  M+   + V+ +  LG  G+ S+ +A L S G     G+ +  ++   P L+LG+G+DD FV++       G  +   + + +P R+ Q  + A + ITITS+TD++ F +G  T          PS+R + LYT   +A TY+  +TFF   +AI        R    C             W+            D +     ++ ++M    D + R +     KI VL+    +  +  WG + ++      +    DS   +++++   YF   P    +   G   +S + +  ++ K   L+ K  N +  D +++ +SWY +F  Y+  N  F + D +  ++ IS
Sbjct:  279 LNIGIMIAFCIITCMMT--DWVKSKPLLGLFGVISAIMAXLFSFGFIIYCGVPFIGINLAAPFLMLGIGIDDTFVML-------GSWRRTSVHETVPARLAQTYRDAAISITITSITDMLSFWIGIITP--------FPSVRIFCLYTGACVAFTYLWHITFFGGCMAIAGYAEKSNRHSLTCLTVIPKSKAESKNWLYRIFCSGGISPDDPFNKKDNAENAIMVIFRDYVARAINNGYFKIFVLVFFTFYLVIAGWGVSNIQEGLERKKLARYDSYSVEFYEMEDTYFREYPYRVNVVLSGHFNYSNLQVQNDVEK---LLKKLENSSFVDHVYT-ESWYRDFLDYVKRNKDFAELDISTEENFIS 624          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000001950 (supercontig:LSalAtl2s:LSalAtl2s1345:18618:29195:1 gene:EMLSAG00000001950 transcript:EMLSAT00000001950 description:"maker-LSalAtl2s1345-snap-gene-0.7")

HSP 1 Score: 83.5741 bits (205), Expect = 3.859e-16
Identity = 76/320 (23.75%), Postives = 147/320 (45.94%), Query Frame = 0

Query:  341 QRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLG------RFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYG-PMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDK-RDGACCWVVHGDQWKPNKLSQRSLMEEAFDAL-GRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFP-GDGEMGTLYFSEMDLYKELPKIKN 650
            + +YGDIS  A+          Y ++++Y+   LG      RF  VE +  LG  G+    L++  S+G+   + +     +  +IP L+L +G D++F++VQ+      +   +G  +   E +G+ +      + +TSVT+   F +G  +         +P++R+++LY    +   +++QVT F++ +++D  R +D   D  CC  V G +    +     ++ + F      F+    V+ +V++    +F      T  +EV       +PDDS + ++F     Y   G      +  S +DL   LP I+N
Sbjct:  586 KETYGDISTIAV---------SYIIMFIYITFSLGENTTWKRF-MVESKITLGLGGVIIVLLSVAASIGIFGFIDVPATLIIFEIIPFLVLAVGXDNIFIMVQT-----CQRDPRGATETHVEHVGRIVGEVAPSMLLTSVTESTCFFLGAISD--------MPAVRAFALYAGMALLIDFLMQVTCFISLISLDMARQNDNYYDIICC--VRGSKKNVRQNGGEGVVYKLFKHFYAPFVMKEWVRAAVVIIFFGWFCTSLAVTPRIEVGLDQKLSMPDDSFVLKYFTFIEKYLSVGPPVYFVVDSSNVDL--SLPGIQN 878          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000006144 (supercontig:LSalAtl2s:LSalAtl2s335:81464:114163:-1 gene:EMLSAG00000006144 transcript:EMLSAT00000006144 description:"maker-LSalAtl2s335-augustus-gene-1.6")

HSP 1 Score: 78.5666 bits (192), Expect = 1.370e-14
Identity = 46/145 (31.72%), Postives = 77/145 (53.10%), Query Frame = 0

Query:  927 VGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSK-SHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNEKPPSYVIAHDAKMEINHNEE 1070
            +G      G+    V +  +I++VG+ V+F+ H+   F+   GSKN R+  +L+ +   VF+G +ST + ++ML  S    VF  +F V   +I+ GLF+GL+FLPV+L + G             PP  ++ HD    +N + E
Sbjct:  878 IGAMGLVLGIHFSAVPAVVLILAVGIGVEFTLHICIGFVTSVGSKNRRIVLSLKHMFTPVFHGAVSTFLGIIMLAFSHFDFVFRYFFLVLSSLILLGLFNGLVFLPVLLLMFG-------------PPGEIVPHDEADFLNSSPE 1009          

HSP 2 Score: 61.6178 bits (148), Expect = 1.865e-9
Identity = 47/181 (25.97%), Postives = 81/181 (44.75%), Query Frame = 0

Query:  456 ERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCWVV---HGDQWKPNKLSQR-------SLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYF 625
            +RIG+ L  AG+ + +T+V +I  F       +        P+LRS++   A  +A      +  F A +  D KR+  +R D  CC+       ++ + +KL+ R       +L   A    G++++   VKI VL+TT      G  G   +E      E +P ++S + + Q    YF
Sbjct:  434 DRIGEVLSRAGMSVLLTAVCNIAAFVSAAVIPV--------PALRSFAFQAAILVACNLPCSLILFPAIMTWDLKRVSARRSDLFCCYSAKDPESNKLEDDKLNVRRFKYDKWTLKYFASRYYGQWISKTPVKILVLVTTTILTCGGLIGLARMEDGLDLTEIVPKNTSFHDFLQAQNKYF 606          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000004370 (supercontig:LSalAtl2s:LSalAtl2s22:157610:161978:1 gene:EMLSAG00000004370 transcript:EMLSAT00000004370 description:"maker-LSalAtl2s22-snap-gene-1.32")

HSP 1 Score: 70.4774 bits (171), Expect = 3.761e-12
Identity = 53/194 (27.32%), Postives = 97/194 (50.00%), Query Frame = 0

Query:  355 DVIFLTCGYFMIYVYVQSMLGRF-NRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPM-HSLIPCLLLGLGVDDMFVIV----QSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACC 541
            DV+ +   YF+++VYV   L  F N+      L   GI     +IL + GL   +G+    +   ++P L+L +G+D++FV+V    +   + EG +       ++   IG  L+     + I+++T    F +G         L  +P++ +++LY  T +   ++ Q+T F+A L++D KRI  K+ D  CC
Sbjct:  404 DVLVVIISYFLMFVYVSFALTDFRNKKYFGITLAIAGIFMVLGSILSTTGLFGFIGVKATMIIFEIVPFLVLAVGIDNIFVLVLTARRKLLSLEGREM------EVDSLIGITLEEIAPAMLISTLTQTCAFYLG--------TLSKMPAVVTFALYAGTSLLINFLAQITGFIAILSLDLKRIKSKKLDLFCC 583          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|123778281|sp|Q0EEE2.1|PTHD3_MOUSE (RecName: Full=Patched domain-containing protein 3; AltName: Full=RND-type protein RNDEu-3)

HSP 1 Score: 101.679 bits (252), Expect = 2.442e-20
Identity = 52/166 (31.33%), Postives = 95/166 (57.23%), Query Frame = 0

Query:  873 QSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQS-GSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLL 1037
            Q++  ++    I +   RN+ +A   + I ++LLI +    + V   +   +V V G M FW + +D ++  N++I +G   DFSAH+++ F+  +  S N++    L  +G  V    IST+I V +L  +K+++F ++FK+ FL++ FG  HGL+F+PV L+  
Sbjct:  741 QAFIYFDQYAAIIENTVRNVMIASTAMFIVSLLLIPHPVCSLWVTFAIASVIVGVTGFMAFWNVNLDSISMINLVICIGFSFDFSAHISYAFVSSTEPSVNKKSIEALYLLGYPVLQSAISTIIGVCVLSAAKAYIFRTFFKIMFLVMFFGAAHGLIFIPVFLTFF 906          

HSP 2 Score: 82.0333 bits (201), Expect = 2.630e-14
Identity = 66/287 (23.00%), Postives = 120/287 (41.81%), Query Frame = 0

Query:  362 GYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKR----------IDDKRDG---ACCWVV------HGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDG 629
             Y +I ++      R + +  +  +   G+ S  ++++   GL   +G+ +  + +  P L+LG+GVDDMF+++ ++  +   +  +       ER+  +     V ITIT++T+++ F  G T++          S++ + +YT T +   Y   +T F A +A+D KR           D K      +CC         HG+   P  L  R       D  G FL     K  V+L  + +     +G   ++          DDS I  +F +   YF   G
Sbjct:  346 AYILIILFAVVSCSRLDCIRNKMCVAVFGVFSVAMSVVSGFGLMLHLGVPFVIIVANSPFLILGVGVDDMFIMISAWQKTSLSESIR-------ERLSNSYSKVAVSITITTITNVLAFYTGITSSFR--------SVQYFCIYTGTTLLFCYFYSITCFGAVMALDGKREVAWSRWLEKPDQKYSSLKKSCCVPFGSLIDKHGEDNHPMNLFFR-------DYFGPFLTTSKAKFIVVLLYIFYIISSIYGCFQVQEGLDLRNLASDDSYITPYFNVEEDYFSDYG 610          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|1042851721|sp|H2L0G5.1|PTC3_CAEEL (RecName: Full=Protein patched homolog 3)

HSP 1 Score: 100.908 bits (250), Expect = 6.923e-20
Identity = 50/169 (29.59%), Postives = 102/169 (60.36%), Query Frame = 0

Query:  874 SYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISY-FKVFFLMIVFGLFHGLLFLPVVLSLLGSKP 1041
            +++ WE    +   LF+ + +  + V     +L+ N  +  +++  VV++ +++GG M   G+ ++ +++  +I +VG+ V+F+AHV   F+   G+ ++R+ S L+ +   V++G IST + V+ML+ S+    ++Y F    L++  G+F+GL  LPV+L+L+G KP
Sbjct: 1130 AFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKP 1298          

HSP 2 Score: 73.1738 bits (178), Expect = 1.650e-11
Identity = 74/300 (24.67%), Postives = 135/300 (45.00%), Query Frame = 0

Query:  265 LGGIQRNSTGHIVGAKA---TFIRWFGRANISEIQNEK--GRNGMDQ---QP------VDVTTLEFEQLL---QISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFN----RVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHS-LIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKR-IDDKRDGACC 541
            +GGIQR++ G +V A+A    F+        + I+N+K     G+D+   QP      +      F + L   +++ + +  +P    S A++   +          + I +  GY ++ +Y     GRF      V+    L   G+    ++ +  +G  + +G+++    + ++P L LGLG+DDMF+++ ++      D+   I       IG  LK  G+ + +TS+ +I+ F  G    I        P+LRS+   TA  +A   +  +  F A + ID +R    KRD A C
Sbjct:  528 IGGIQRDN-GKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQF-------NYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNY------DEIINICNK--NEIGVLLKETGMSVMLTSINNILAFISGYVLPI--------PALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYC 803          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|83305902|sp|O15118.2|NPC1_HUMAN (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 96.6709 bits (239), Expect = 1.219e-18
Identity = 56/188 (29.79%), Postives = 106/188 (56.38%), Query Frame = 0

Query:  868 TFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLI-ANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQ-SGSKNERMRSTLRKIGPAVFNG-GISTLIAVLMLMNSKSHVF-ISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNEK 1051
             F  +  Y  +E    I  +   NL +++  + + TM+L+     S +I+   + M LV++ G M+ WG++++ V+  N+++S G+ V+F +H+   F +   GS+ ER    L  +G +VF+G  ++    +++L  +KS +F I YF+++  M++ G  HGL+FLPV+LS +G    K    + E+
Sbjct: 1078 VFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEE 1265          

HSP 2 Score: 78.1814 bits (191), Expect = 5.234e-13
Identity = 58/211 (27.49%), Postives = 108/211 (51.18%), Query Frame = 0

Query:  341 QRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNR-----VEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMH----SLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACC 541
            +RS  D  N     DV  +   Y ++++Y+   LG         V+ +  LG  GI     ++  S+G+ S +GL   P+      +IP L+L +GVD++F++VQ++   E   + +G  + L +++G+ L      + ++S ++ V F +G         L V+P++ ++SL+    +   ++LQ+T FV+ L +D KR +  R D  CC
Sbjct:  606 ERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGL---PLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDE---RLQG--ETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGA--------LSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCC 800          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|6225892|sp|Q61115.1|PTC1_MOUSE (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 94.7449 bits (234), Expect = 4.335e-18
Identity = 71/294 (24.15%), Postives = 136/294 (46.26%), Query Frame = 0

Query:  265 LGGIQRNSTGHIVGAKA------------TFIRWFGRANISEIQNEKGRNGMDQQPVDVTTLEFEQLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHS-LIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLP--ERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCW 542
            +GG  +N+TG +V A A             +  + G   +S I   + R     +    T +E           +   P    +  ++ +S+ D+S       VI +  GY ++  Y    + R++  + +  +G  G+    L++   +GLCSL+G+ +    + ++P L LG+GVDD+F++  +F+ +       G  K +P  +R G+ LK  G  + +TS++++  F +     I        P+LR++SL  A  +   + + +  F A L++D  R +D+R D  CC+
Sbjct:  329 VGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVS-------VIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSET-------GQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPI--------PALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCF 600          

HSP 2 Score: 74.3294 bits (181), Expect = 8.513e-12
Identity = 50/165 (30.30%), Postives = 85/165 (51.52%), Query Frame = 0

Query:  878 WEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISY-FKVFFLMIVFGLFHGLLFLPVVLSLLGSKP 1041
            WE    +   L  ++ + + C  +   + + N  +  I+++ + +  V++ G M   G+ +  V    +I SVG+ V+F+ HVA  F+   G KN R    L  +   V +G +STL+ VLML  S+    + Y F V  ++ V G+ +GL+ LPV+LS  G  P
Sbjct: 1004 WEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCP 1168          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|6225890|sp|Q90693.1|PTC1_CHICK (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 94.3597 bits (233), Expect = 6.973e-18
Identity = 56/208 (26.92%), Postives = 109/208 (52.40%), Query Frame = 0

Query:  339 NVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHS-LIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLP--ERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCW 542
            ++ +S+ D+S       VI +  GY ++  Y    + R++  + +  +G  G+    L++   +GLCSL+G+ +    + ++P L LG+GVDD+F++  +F+ +       G  K +P  +R G+ LK  G  + +TS++++  F +     I        P+LR++SL  A  +   + + +  F A L++D  R +D+R D  CC+
Sbjct:  429 DILKSFSDVS-------VIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSET-------GQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPI--------PALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCF 614          

HSP 2 Score: 74.7146 bits (182), Expect = 6.032e-12
Identity = 49/165 (29.70%), Postives = 85/165 (51.52%), Query Frame = 0

Query:  878 WEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISY-FKVFFLMIVFGLFHGLLFLPVVLSLLGSKP 1041
            WE    +   L  ++ + + C  +   L + N  +  I+++ + +  V++ G M   G+ +  V    +I SVG+ V+F+ H+A  F+   G KN R    L  +   V +G +STL+ VLML  S+    + Y F V  ++ + G+ +GL+ LPV+LS  G  P
Sbjct: 1017 WEQYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYP 1181          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|449081276|sp|O35604.2|NPC1_MOUSE (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 93.5893 bits (231), Expect = 1.027e-17
Identity = 46/141 (32.62%), Postives = 89/141 (63.12%), Query Frame = 0

Query:  901 ITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQS-GSKNERMRSTLRKIGPAVFNG-GISTLIAVLMLMNSKSHVF-ISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            +T ++L     S +I+ + + M LV++ G M+ WG++++ V+  N+++S G+ V+F +H+   F + + GS+  R    L  +G +VF+G  ++    +++L  +KS +F I YF+++  M++ G  HGL+FLPV+LS +G
Sbjct: 1111 VTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIG 1251          

HSP 2 Score: 72.0182 bits (175), Expect = 3.730e-11
Identity = 65/297 (21.89%), Postives = 131/297 (44.11%), Query Frame = 0

Query:  341 QRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNR-----VEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMH----SLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQ-----LHTG 623
            +RS  D  N     DV  +   Y ++++Y+   LG         V+ +  LG  GI     ++  S+G+ S MG+   P+      +IP L+L +GVD++F++VQ++   E   +     + L +++G+ L      + ++S ++   F  G         L  +P++ ++SL+    +   ++LQ+T FV+ L +D KR +       C V   D  + +  S+  L     +     L    ++  V+   +   +        +++       +P+DS +  +F+     LH+G
Sbjct:  606 ERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGM---PLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQE-----ETLDQQLGRILGEVAPTMFLSSFSETSAFFFGA--------LSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSG 886          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|160415977|sp|Q13635.2|PTC1_HUMAN (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.030e-17
Identity = 72/296 (24.32%), Postives = 139/296 (46.96%), Query Frame = 0

Query:  265 LGGIQRNSTGHIVGAKA------------TFIRWFGRANISEIQNEKGRNGMDQQPVDVTTLEF--EQLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHS-LIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLP--ERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCW 542
            +GG  +NSTG +V A A             +  + G   +S I   + +     +    T +E   + + Q S      +     +  ++ +S+ D+S       VI +  GY ++  Y    + R++  + +  +G  G+    L++   +GLCSL+G+ +    + ++P L LG+GVDD+F++  +F+ +       G  K +P  +R G+ LK  G  + +TS++++  F +     I        P+LR++SL  A  +   + + +  F A L++D  R +D+R D  CC+
Sbjct:  343 VGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTT--TTLDDILKSFSDVS-------VIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSET-------GQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPI--------PALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCF 614          

HSP 2 Score: 75.0998 bits (183), Expect = 4.889e-12
Identity = 48/153 (31.37%), Postives = 83/153 (54.25%), Query Frame = 0

Query:  892 LFLAII--CVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISY-FKVFFLMIVFGLFHGLLFLPVVLSLLGSKP 1041
            LF++++  C  +   + + N  +  I+++ + +  V++ G M   G+ +  V    +I SVG+ V+F+ HVA  F+   G KN R    L  +   V +G +STL+ VLML  S+    + Y F V  ++ + G+ +GL+ LPV+LS  G  P
Sbjct: 1030 LFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYP 1182          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|8134591|sp|P56941.1|NPC1_PIG (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 92.4337 bits (228), Expect = 2.181e-17
Identity = 45/141 (31.91%), Postives = 90/141 (63.83%), Query Frame = 0

Query:  901 ITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQS-GSKNERMRSTLRKIGPAVFNG-GISTLIAVLMLMNSKSHVF-ISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            +T +L+     + +I+ + + M LV++ G M+ WG++++ V+  N+++S G+ V+F +H+   F L + GS+ +R    L  +G +VF+G  ++    +++L  +KS +F I YF+++  +++ G  HGL+FLPV+LS +G
Sbjct: 1111 VTVVLMGCELWATVIMCVTIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTLSTKGSRVDRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAIVLLGATHGLIFLPVLLSYIG 1251          

HSP 2 Score: 82.0333 bits (201), Expect = 3.190e-14
Identity = 79/303 (26.07%), Postives = 143/303 (47.19%), Query Frame = 0

Query:  341 QRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNR-----VEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMH----SLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFL----AYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFL--PDDSSIYQWFQ-----LHTG 623
            +RS  D  N     D+  +   Y ++++Y+   LG         V+ +  LG  GI     ++  S+G+ S +G+   P+      +IP L+L +GVD++F++VQ++   E   + +G  + L +++G+ L      + ++S ++ V F +GG        L V+P++ ++SL+    +   ++LQ+T FV+ L +D KR +  R    C V   +     + S+  L     ++    L      PIV I+V +  L+F         L +VE    + L  PDDS +  +FQ     LH G
Sbjct:  606 ERSIEDELNRESNSDLFTILISYAIMFLYISIALGHIKSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYIGV---PLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDE---RLQG--ETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGG--------LSVVPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQGAEDGAGVQASESCLFRFFKNSYAPLLLKDWMRPIV-IAVFVGVLSFSI-----AVLNKVEIGLDQSLSMPDDSYVMDYFQSLSRYLHAG 886          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|6225894|sp|O35595.2|PTC2_MOUSE (RecName: Full=Protein patched homolog 2; Short=PTC2)

HSP 1 Score: 91.6633 bits (226), Expect = 3.319e-17
Identity = 53/206 (25.73%), Postives = 104/206 (50.49%), Query Frame = 0

Query:  339 NVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHS-LIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCW 542
            ++ R++ ++S   +         GY ++  Y    + R++  + +  +G  G+    LA+   +GLC+L+G+ +    + ++P L LG+GVDD+F++  +F  +  +         LPER+G+ L+  G  + +TSV ++V F +     I        P+LR++SL  A  +   +   +  F A L++D +R   +R D  CC+
Sbjct:  385 DILRAFSEVSTTRV-------VGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTP-------LPERMGECLRSTGTSVALTSVNNMVAFFMAALVPI--------PALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCF 568          

HSP 2 Score: 76.6406 bits (187), Expect = 1.514e-12
Identity = 43/177 (24.29%), Postives = 85/177 (48.02%), Query Frame = 0

Query:  837 FVHRKFNSSTQHIPAMNWVKDSVVDAGFPGI-TFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYF 1012
            F+      +   + A+   + +  +AG  G+  +     +  WE    + +     + + ++C  +   LL+ +  +  +++L + M  V++ G M F G+ +  +    ++ S+G+ V+F+ HVA  F+   GS+N R  S L +    V +G +STL+ +LML  S     I YF
Sbjct:  912 FLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYF 1088          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|73921247|sp|Q6T3U3.1|NPCL1_RAT (RecName: Full=NPC1-like intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1-like protein 1; Flags: Precursor)

HSP 1 Score: 88.1965 bits (217), Expect = 4.034e-16
Identity = 73/297 (24.58%), Postives = 131/297 (44.11%), Query Frame = 0

Query:  339 NVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNR-----VEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYG-PMHSLIPCLLLGLGVDDMFVIV---QSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKR-DGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYF 625
            + +RS  D  N     D+      Y ++++Y+   LG ++R     VE +A LG  G+     A+L +MG  S +G+     +  ++P L+L +G D++F+ V   Q      GE +           IG+ L      + + S+++ + F +G  T         +P++R+++L +   I   ++LQ+T FVA L++D KR +  R D  CC+     +  P K  +  L+         FL +  ++  V+L  L  F    +    + V       LP DS +  +F     Y 
Sbjct:  616 SAERSLEDEINRTTIQDLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREA--------HIGRTLGSVAPSMLLCSLSEAICFFLGALTP--------MPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTR--KLPPPKEKEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYFLFLNRYL 894          

HSP 2 Score: 60.8474 bits (146), Expect = 9.540e-8
Identity = 56/187 (29.95%), Postives = 100/187 (53.48%), Query Frame = 0

Query:  901 ITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSG-SKNERMRSTLRKIGPAVFNG-GISTLIAVLMLMNSKSHVF-ISYFKVFFLMIVFGLFHGLLFLPVVLSLLGS-------KPYKIADTSNEKP----PSYVIAHDAKMEINHNEEQDL 1073
            +  +LL  +  S I+ +L ++M LVD  G M  WG++ + V+  N++ +VG+ V+F +H+   F + +  ++ ER +     +G AVF G  ++    +L+L  +++ +  I +F++  L+ + GL HGL+FLPVVLS LG        +  K+A  +   P    P Y    DA   +N+    +L
Sbjct: 1127 VCYLLLGLDMCSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLGPDVNQALVQEEKLASEAAVAPEPSCPQYPSPADADANVNYGFAPEL 1313          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325286696|ref|XP_023328081.1| (Niemann-Pick C1 protein-like [Eurytemora affinis])

HSP 1 Score: 526.939 bits (1356), Expect = 5.253e-160
Identity = 287/753 (38.11%), Postives = 440/753 (58.43%), Query Frame = 0

Query:   16 SDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDLS--SVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVES-------------------SDLPSKPVQKREAPSPNTEEDFFG-----DFGDESDDFAAIKSGAGVGKA--FDPSVVFYPEQYCPIVEAMPMA-CFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQQPVDVTTLEFEQLLQISLDDKCGYPEGLKSQA-NVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESY-LNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTV 737
            S+ I   +E +FY +G  +A +P  +I   LV   LC +GL R  +E N  KLWIP +SD+  N++WLW+NFP D+   SVIL  + N+L PE I+++Y++H+ ++ + +S G+ W D+CY++P+VD+E                     ++P    Q ++  S  T +  F         D S   ++  S   + +A  FDP++  YPE YCP++E +  + C E S+LE++ + G +T + +  I+ L QE+++ A+NT+  S ++ R +DFSKYLG I RNS+GHI+GA+A F+R+FG+ N + + + +        PVD  TL +E  +   +D      +G  S   NV +S+ D+S GAI+ D +    G   ++VYVQ MLG+FN V+ R  L ++G+   GLAIL S G CS +GL + P+HS+IP L++GLGVDDMFVI+QS +N +   K    +  +  +I + +K  GV ITITS TD++ FA+G  T        VLP+L+S+ L+ A GIA+ Y    T FVA+L+ID++RI  KR+G C  +VH D W PNK+SQR    E F+ LG  L+ P V+  VL+ T      G +G + L+ EF  ++F+P +S +  WF  +T YFP +GE   +   ++D   EL K++NLV    NET+I+ + DSWY EF  + +    ++  +       + E F   LTQFLFSP GA +  +F F   + CG  AP VL+S++
Sbjct:   28 SETIVHSMELFFYWYGSILARYPIRSICLTLVIPGLCAIGLLRLEQENNPYKLWIPQDSDYIRNHEWLWQNFPDDIRFHSVILTGD-NVLTPEYIRKLYEVHEAMVNVTSSKGERWSDICYRVPMVDLEPIQDLLNDSLYEYEDYEDDVEEIPETTEQIQDGGSETTADSMFDITTQISVIDNSTLISSNNSENRIKRASEFDPAIDLYPEDYCPLIEDLTTSICLENSLLELWGKAG-YTQETKDIIYALTQEDILRAINTVTTSPVYDRAQDFSKYLGDISRNSSGHIIGARAMFLRFFGQVNTTALTDSEDVAVTKGSPVDGNTLAWEDAI---IDVLSKLADGDSSLVFNVAKSFSDLSAGAIYGDALLFGIGTSTVFVYVQIMLGKFNFVQTRPGLSSVGLGCYGLAILTSYGFCSGIGLVFSPLHSVIPFLIMGLGVDDMFVIMQSLDNLDA--KGGTALWSIERKIAETMKLGGVAITITSFTDLIAFAIGAIT--------VLPALKSFCLFCAVGIASVYFYTATIFVAFLSIDERRIRAKRNGMCPLIVHKD-WTPNKISQREFGRELFEKLGWVLSNPFVQAFVLIATAVLGGFGIYGLSELKQEFNPIKFIPPESYLAGWFHANTEYFPKEGERVIVNIGDIDYNNELHKLENLVGALVNETEILSAVDSWYLEFRKFAVGKNLVEESDWFNLFQENREKFYTILTQFLFSPDGAPYRRRFNFVNDLICGEAAPTVLLSSI 764          

HSP 2 Score: 229.18 bits (583), Expect = 3.249e-57
Identity = 110/212 (51.89%), Postives = 165/212 (77.83%), Query Frame = 0

Query:  832 MSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYK 1043
            +S++E  HR F S ++ IPAMN +K+ V +A F    F     Y+ WE DEVI  EL++N+ ++++CV +T +LLIA+  +C++V++CVV+SLV+V G M+FWGLT+D+++  N+ I++GL VD+SAHVAH F++Q GSK+ERMRSTL +IGPAV NGG ST +A +  + SKSHVFIS+F++F L+++FGL+HGL+FLPV L+L+G +P++
Sbjct:  761 LSSIELTHRLFTSPSEWIPAMNRIKELVREANFSSRAFPTGTEYASWETDEVIGYELYQNMGISLLCVFLTVLLLIASIRACLLVLVCVVLSLVNVAGYMHFWGLTVDVISCVNLEIAIGLTVDYSAHVAHTFLIQKGSKSERMRSTLTEIGPAVLNGGFSTFLAFIFTVGSKSHVFISFFRIFMLVVLFGLYHGLIFLPVALALIGPEPHQ 972          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261885|ref|XP_023331667.1| (patched domain-containing protein 3-like isoform X1 [Eurytemora affinis] >gi|1325261897|ref|XP_023331674.1| patched domain-containing protein 3-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 520.005 bits (1338), Expect = 2.707e-159
Identity = 288/740 (38.92%), Postives = 428/740 (57.84%), Query Frame = 0

Query:    6 QAIPNGLQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDL--SSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESS-DLPSKPVQKREAPSPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISE--IQNEKGRNGMDQQPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLN--DYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTV 737
            ++ P  L K   +  + LED F  +G++VA HP + IL  +    +CG GL+ F  E   VKLWIP NSD+  NN W++EN+P DL  +S+IL  E N+L  +V++ MY++ K++  +R    + W D C K PV+ V S  D+  +  ++RE      ++D FGD  DE  D   I     +   F  S+  YP+ YC IV  MP+AC E+SILE+F QGG +  + +  +  L  E ++  +N    SGIFL   +F+ YL  I+ N+ G I+GAKAT+I W GR N++E  +    GR     +P+ ++ L++E  +L++ L+ K  YP+GL+S  NV RS+GDI+   I  D+ F   GY M++ YVQ MLG+F+ VE R +L  +GI    + I+VS G+CS  G  YGPMHS++P L+LG+G+DDMFVI+Q ++    E+K + ++K    R G  +K AG  IT+TS TD++ F VG  T        +LP+L+S+ +Y A GI AT+V Q +FF+AW+ +DQ+RI+  R+G C   VH +     +  ++  ++  F     +L    VKISVLL  L     G +G  LL  EF    FLP D+ I QWF  +  YFP  GE GT+YFS  +L  ++ KI+ L      +TDII   D+W   +  YL   +Y  +P +      +++  F   L+QFLFSP+GA + N F F E + CG  A +V +S +
Sbjct:   11 ESAPGLLNKLQGSTLRILEDVFQSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFNSLILTGE-NVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDIFGR--KRREI---KFDDDLFGDEFDEEKD-TVITDPLKLATQF--SIDEYPDPYCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYNTQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRG----EPISMSMLQWEGDMLEVMLN-KSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVK----RFGFLMKQAGAAITVTSATDVIAFGVGAFT--------ILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPCYVHPETNNQTQ-ERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTELPADIDKIEKLSQDLREQTDIIVKSDAWTRPYIDYLQKLNYTEKPSK------LNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEI 717          

HSP 2 Score: 248.054 bits (632), Expect = 6.722e-64
Identity = 109/207 (52.66%), Postives = 155/207 (74.88%), Query Frame = 0

Query:  832 MSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            +S + + HR  N   +H+PAMN VK+ +  A   G  FAMA  Y+ WE DE+IS E++RN+ LAI+CV ITT+L I+N    +++++CV ++LVDVGG M+FWGLTID V+  N+II++GLCVD+SAHV H F+ + G + +R+  T+  IGPAVFNGG+ST +A ++L  S+SHVF S+FK+FFL++ FGLFHGL+ LPV+LS +G
Sbjct:  714 LSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIG 920          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261904|ref|XP_023331682.1| (patched domain-containing protein 3-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 519.235 bits (1336), Expect = 4.654e-159
Identity = 288/740 (38.92%), Postives = 428/740 (57.84%), Query Frame = 0

Query:    6 QAIPNGLQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDL--SSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESS-DLPSKPVQKREAPSPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISE--IQNEKGRNGMDQQPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLN--DYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTV 737
            ++ P  L K   +  + LED F  +G++VA HP + IL  +    +CG GL+ F  E   VKLWIP NSD+  NN W++EN+P DL  +S+IL  E N+L  +V++ MY++ K++  +R    + W D C K PV+ V S  D+  +  ++RE      ++D FGD  DE  D   I     +   F  S+  YP+ YC IV  MP+AC E+SILE+F QGG +  + +  +  L  E ++  +N    SGIFL   +F+ YL  I+ N+ G I+GAKAT+I W GR N++E  +    GR     +P+ ++ L++E  +L++ L+ K  YP+GL+S  NV RS+GDI+   I  D+ F   GY M++ YVQ MLG+F+ VE R +L  +GI    + I+VS G+CS  G  YGPMHS++P L+LG+G+DDMFVI+Q ++    E+K + ++K    R G  +K AG  IT+TS TD++ F VG  T        +LP+L+S+ +Y A GI AT+V Q +FF+AW+ +DQ+RI+  R+G C   VH +     +  ++  ++  F     +L    VKISVLL  L     G +G  LL  EF    FLP D+ I QWF  +  YFP  GE GT+YFS  +L  ++ KI+ L      +TDII   D+W   +  YL   +Y  +P +      +++  F   L+QFLFSP+GA + N F F E + CG  A +V +S +
Sbjct:   11 ESAPGLLNKLQGSTLRILEDVFQSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFNSLILTGE-NVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDIFGR--KRREI---KFDDDLFGDEFDEEKD-TVITDPLKLATQF--SIDEYPDPYCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYNTQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRG----EPISMSMLQWEGDMLEVMLN-KSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVK----RFGFLMKQAGAAITVTSATDVIAFGVGAFT--------ILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPCYVHPETNNQTQ-ERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTELPADIDKIEKLSQDLREQTDIIVKSDAWTRPYIDYLQKLNYTEKPSK------LNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEI 717          

HSP 2 Score: 247.284 bits (630), Expect = 1.024e-63
Identity = 109/207 (52.66%), Postives = 155/207 (74.88%), Query Frame = 0

Query:  832 MSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLG 1038
            +S + + HR  N   +H+PAMN VK+ +  A   G  FAMA  Y+ WE DE+IS E++RN+ LAI+CV ITT+L I+N    +++++CV ++LVDVGG M+FWGLTID V+  N+II++GLCVD+SAHV H F+ + G + +R+  T+  IGPAVFNGG+ST +A ++L  S+SHVF S+FK+FFL++ FGLFHGL+ LPV+LS +G
Sbjct:  714 LSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIG 920          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1059386504|ref|XP_017768460.1| (PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386507|ref|XP_017768461.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386509|ref|XP_017768462.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386511|ref|XP_017768463.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386513|ref|XP_017768464.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386516|ref|XP_017768465.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides] >gi|1059386518|ref|XP_017768466.1| PREDICTED: protein patched homolog 3-like [Nicrophorus vespilloides])

HSP 1 Score: 496.893 bits (1278), Expect = 2.707e-151
Identity = 325/1040 (31.25%), Postives = 514/1040 (49.42%), Query Frame = 0

Query:   13 QKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDLSSVILE-HEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRW-----FGRANISEIQNEKGRNGMDQQPVDVTTLEFEQLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDALGR--FLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTVVDVEFPICLSKLFS-PAYDLPWLNIWVNATLQIAKLLDVRRPQSLVKEGTCTLSVALGEHWRINHVHDRVIHKMTGKTCLLTFSCVDPYTTLYPPMSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFM-LQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPY 1042
            Q+ + ++    E +FY+ G  +A  P +TI    + V L   GLFRF++EK+ +KLW+PPNS F  +++ L E F       +++   +++L PEV  +++++ + V    T  G  W D+C+K+P +D     L   P ++                G+ ++D             FDPS+    + YC ++E +   CFE S+L+++          +  I  L ++ ++  +N      +  + +++   LGG+  NSTGHI+ AK+    W     F   N+ EI N  G N           LE+E+     +       E  K      RS+GDIS   ++ D+  L  G F++  YVQ ++ +FN VE R  LG  G+ S G+A +V+ GLCSL G+ YGP+H+ +P LL+GLGVDDMFVI+  +     E K    +  LPE++G  +KHAGV IT+T+ TD++ F +G +T        +LP L+SY +Y A G+  T++  VTF+VA   IDQ+R++    G   W  + + +KPN  SQ     + F+ +     L +P  K+ VL  TL    L       LE  F    F+P  + + ++ +    YFP +G     Y   ++   E   + ++  +  N +DI+   +SW   F +Y+    +     I +  + E  FN+ L++FLFS  GAQ    F+F +P+ CG  APK+++S+ +D +F     KLFS P   +P                 +RR  S+ K+                                + F+  D ++T +          + F S         W+ D V+DA                        EL RNL LA+I V   TMLLIAN   C  V +C+++S+V+V G M  WGLTID+V    + +++GLCVD++ H+ H F+ +  GS N+R   T+  IG AV  GG STLI V ML  S ++ F ++FK+F L+++FGLFHG++ LPV+LS +G KPY
Sbjct:   12 QRFTYSVVTYTELFFYKLGIRIAKSPFITIALCWMFVILSAFGLFRFHQEKSPIKLWVPPNSQFVADSERLMETFETAYRIQMMQIVADDVLTPEVFMELHEVDELVRNSVTRSGIHWNDVCFKIPQIDQGMYTL-MMPYRR----------------GNNTED-------------FDPSLEMDTDTYCGMIEGIEKECFEKSVLDLWDHS-------DRKIKKLTKQAIVDKINKFKIDPLLGQLKNYGDLLGGVTTNSTGHIIAAKSLNNYWMALLNFSAVNMDEIGNSAGTNDW----ASAECLEWEETFLNIMKYSAQRAENFKIYYTAARSFGDISGATMFQDLDILFIGVFIMVFYVQFVISKFNCVEARMILGCFGLLSVGMAFVVAAGLCSLFGVFYGPVHTSLPFLLMGLGVDDMFVIMACWEQLTPEQK----LLPLPEKVGAMMKHAGVSITVTTATDVLAFFIGSST--------ILPCLQSYCIYAALGVLMTFIFVVTFYVACFTIDQRRVEKHISGFWPWKRY-ENYKPNPFSQAETSNKVFNLIYSKVILTWP-GKLLVLCITLICLGLSIESVFKLEQRFDPTWFIPQHTYLGEFLKQKFEYFPNNGFEAATYVGAVNYTYEFKNVIHIDSQLENSSDIVTDINSWVGPFRNYVK---VNYKIDIYNETLEETKFNDFLSKFLFSMKGAQFQGNFKFEKPLQCGIPAPKIMMSS-IDYKF-----KLFSGPEEHVP----------------AMRRSMSISKD--------------------------------VNFTTGDRFSTAFS---------KVFAS---------WITDEVIDA------------------------ELLRNLQLALIAVMACTMLLIANLQICFWVFICILLSIVNVCGFMQRWGLTIDLVTCIGLTLAIGLCVDYATHIGHTFLTIGEGSLNDRALKTVTSIGSAVLYGGTSTLIGVAMLGVSDAYNFQTFFKIFLLVVIFGLFHGVVLLPVILSCIGPKPY 897          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1061485453|gb|ODN02586.1| (Patched domain-containing protein 3, partial [Orchesella cincta])

HSP 1 Score: 483.411 bits (1243), Expect = 5.205e-145
Identity = 280/739 (37.89%), Postives = 419/739 (56.70%), Query Frame = 0

Query:   12 LQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFP-PDLSSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFGD--FGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVN--TIGQ--SGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGR-----ANISEIQNEKGRNGMDQQPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDAL-GRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVST 736
            LQ  S  I   LE +FYR G  +   P   I+  ++   LCG+GL RF  E    KLWIP NS+F  + +WL +NFP P      +   E++L P+V+  + +IH  V ++  S  + W D+C+KLPV+  +++       +KRE  + N     F     G +S         A   K FDPSV    + YC IV ++   CFE+++LE++           + I+ L +EE++ A+N   I Q    +F R  +++  LG +QRN +G+I+GA+A  IRWF R      N+ ++ N++G      Q VD     +E +L +  L  +   PEG+    NV RS+ DIS   I  D   L  G+ ++++YV  MLG+FN VEQR  L   G++  GLAILVS G+CS   + YGP+H+++P LLLG+G+DDMFVI+Q +NN   ED  K I    PE++G  L+HAGV ITITS+TD   FA+GGTT        VLP+L+S+ +Y A GI ATY+ Q TFFVAWLAIDQKRI+ +R+    +++H   + P  L  RS  + A + +  + L     K+ V L T   F +  WG  LL  EF  + FLP DS ++++F     +FP  GE G +  +++DL   LPK+++L+  F N T  +   D+WYP F+ Y+N  F    + IP+  ++ + FN+ L +FLFSP G ++ ++F+F + + CG  AP V++S+
Sbjct:   56 LQLISLKIVGSLERFFYRWGYQIGLTPVPFIVACVILTVLCGIGLLRFTPENRPDKLWIPWNSEFVKDTEWLNKNFPSPFRFHYAIITAEDVLQPKVLDVLLEIHNKVKEIEASTYK-WKDICFKLPVIKFDTNKR-----RKREVNNANNSATLFNGNFDGFDSAFDFDDDEEATSEKPFDPSVSLPTDVYCNIVSSLDKECFELNMLELWNYN-------SSVINKLTKEEIVNAINHEQIRQVFYPVFGRPVNYATELGKLQRNESGYIIGAEAVMIRWFARLNRTAMNLGDVLNDEGTG----QAVDKYNFMWEKELSKFFLSFRP--PEGVNIYFNVARSFADISGSTIMGDASMLAVGFSIVFLYVIFMLGKFNFVEQRPLLSLAGLSCVGLAILVSYGICSAFKIIYGPVHNILPFLLLGIGIDDMFVIMQCWNNLRPEDMKKSI----PEKLGLTLQHAGVSITITSLTDFFAFAIGGTT--------VLPALQSFCVYAALGIVATYIFQATFFVAWLAIDQKRIERRRNAFIPYIIHA-SFSPKTLMTRSFAQTAIEKIYCKVLFRKFSKVMVFLVTFGVFGVNVWGNILLRQEFNPIWFLPQDSYLFKYFAARDQFFPEKGEEGFILMNDVDLNSNLPKLESLLLDFENST-FVHEIDAWYPPFKQYVNSNF---GKDIPNQKLTNKEFNKYLGKFLFSPTGGKYQSRFKFDKNLTCGKSAPAVMLSS 758          

HSP 2 Score: 248.44 bits (633), Expect = 1.087e-63
Identity = 113/226 (50.00%), Postives = 168/226 (74.34%), Query Frame = 0

Query:  832 MSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFPGI---TFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIA---DTSNEK 1051
            +S+  F    F+   QH+PAMN +K  + +A F      TF  + +++ WE DE+I+ EL+RN+ LA+ICV +TT++LIAN  +C +V+LCV+++LVDVGG+M+FW LTID V+  ++++++GLCVD++AH+AH FM   G++NER    L  IGPAV NGG ST +A ++L NSKSHVF+++FK+FFL++V+GLFHGL+FLPV+LS++G  PY  A   D +N+K
Sbjct:  756 LSSANFKFNIFSGPEQHVPAMNNIKRIIREANFTPANSHTFIWSFAFAGWETDEIITDELYRNMGLAMICVFLTTLVLIANLQTCCMVLLCVILTLVDVGGSMHFWNLTIDTVSCIDLVLAIGLCVDYAAHIAHAFMTSVGTRNERSTKALVNIGPAVLNGGFSTFLAFILLANSKSHVFLTFFKIFFLVVVYGLFHGLVFLPVLLSIIGPAPYATAVLEDENNDK 981          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1022768415|gb|KZS13150.1| (Niemann-Pick C1 protein [Daphnia magna])

HSP 1 Score: 457.218 bits (1175), Expect = 7.253e-136
Identity = 267/732 (36.48%), Postives = 406/732 (55.46%), Query Frame = 0

Query:   12 LQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFPPDL---SSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFGDFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGGIQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQ-QPVDVTTLEFEQLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGDQWKPNKLSQRSLMEEAFDA-LGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIP--DHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTD-APKVLVS 735
            + +AS       E++F R G  VA +P   I+ ++V  A C +GL  F  E    KLWIP +S F  N++WL ENFP ++   S V+++  +N+L PEV+  M ++H N+  + ++ G  W D+C+K+PVV  + ++   K         P   EDF  +F     +F  + S + +   F+P++      +C IVE +  AC E +IL++++   +        I  L +E++I  +NT+ +S   L    +S  LG  + N TGHIV A +  ++ F R N S I N+   N     + VD   LE+E+     L +    P   K      RS+GDIS+  I  DV  L  G  ++++YVQ MLG+FN VEQRAFL   GI S  +AI+VS GLCS  G+ YGP+HS++P LLLG+G+DDMFVIVQ ++N   E+  +     LP RIG  +KHAGV ITITSVTD   FAVG TT        VLPSLRS+ +Y+A GI ATY+ Q +FFVAWL +DQKRI+  RDG     VH + WKP+ LS+   ++  F+  L ++L     K+ +L+     F +  WG+ L+  EF  + F+P  + + Q+F +   +FP +G+M ++Y    +L   L ++  L+D   NET+I+   D W+  F+      F     AI   +  +++E F+  L  +LF+  GA+    F+F +P+ C +  AP + ++
Sbjct:   23 VNRASFIFMANFENFFLRWGITVAKNPWTYIIASVVIAATCSIGLLNFTAENRPTKLWIPQDSGFVQNSEWLQENFPNEIRLQSWVVVD--DNVLRPEVLLWMLELHDNITAVSSASGYQWRDVCFKVPVVTEQQTNQRRKRQLNISENDPFDFEDF--NFDGFDANFEEMPSSS-IDPDFEPAIDLPTGIFCDIVEGLEEACLENNILDIWSYKKE-------VISKLSREDIINTINTVQKSN--LTRVQYSSLLGQPRFNETGHIVSAGSLLVQLFSRVNYSLIANDVTDNDAGTGEVVDPINLEWEKAFVNLLHNFTSPPNSSKLYYMAARSFGDISSATILGDVRLLAAGICVVFIYVQLMLGKFNLVEQRAFLSLTGIASVAMAIVVSYGLCSAFGVFYGPVHSILPFLLLGIGIDDMFVIVQCWSNLNKEEMKRS----LPVRIGITMKHAGVSITITSVTDFAAFAVGATT--------VLPSLRSFCIYSAVGILATYIFQASFFVAWLTLDQKRIESHRDGMIPCFVHRN-WKPSSLSRIEPLQLFFNRFLAKYLFKWPAKVFILVLAAFLFGINTWGSVLMRQEFNPLWFIPTSTYLSQYFSVIESHFPDNGQMASIYIQTTNLSSNLDRLDALIDTVRNETNIVSQVDDWFGGFKE-----FTAKRHAIDWMNQNMTDEQFSTYLKNYLFTQKGAKFRRNFKFEDPLQCRSAIAPPISIT 722          

HSP 2 Score: 232.261 bits (591), Expect = 1.270e-58
Identity = 124/261 (47.51%), Postives = 183/261 (70.11%), Query Frame = 0

Query:  826 TTLYPPMS--TMEFVHRKFNSSTQHIPAMNWVKDSVVDAG--FPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNEKPPSY----VIAHDAKMEINHNEEQDLVDIQK 1078
            + + PP+S    +FV R F+   +HIPAM  +KD   D    F    FA A+ +S WE DEVI +EL+RNL +A++CV ITT +LIAN  +C++V+LCVV++LV V G M+FWGLTID V+  N+++++GLCVD++AHVAH FM ++G++NER  +T+  IGPAVF+GG ST +A + L NS SHVFI++FK+F L++ +GLFHGLLF PV+LSL+G  P+ +     ++PP+     V A + + ++ H E+  +V + K
Sbjct:  713 SAIAPPISITNFDFVFRPFSGREEHIPAMRRIKDIAEDFSQQFGSQVFASARIFSSWETDEVIMEELYRNLAIAMVCVFITTFILIANLFACVLVLLCVVLTLVCVNGTMHFWGLTIDTVSCINLVLAIGLCVDYAAHVAHTFMTKTGTRNERAAATVSSIGPAVFHGGFSTFLAFIFLANSDSHVFITFFKIFVLVVAYGLFHGLLFFPVILSLIGPPPFDL-----DRPPNLEDKAVTAEELEPQLTHREKSAIVGVLK 968          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228378754|ref|XP_021950763.1| (Niemann-Pick type protein homolog 1B-like [Folsomia candida] >gi|1215270797|gb|OXA57108.1| Niemann-Pick C1 protein [Folsomia candida])

HSP 1 Score: 430.639 bits (1106), Expect = 9.822e-126
Identity = 258/772 (33.42%), Postives = 418/772 (54.15%), Query Frame = 0

Query:   12 LQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFP-PDLSSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVE-------------SSDLPSKPV-QKREAPSPNTEEDFFGDFGDESDD------FAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGG-IQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQ-QPVDVTTLEFEQ-LLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHGD-QWKPNKLSQRSLMEEAFDA-LGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTVVDVEFPICLSKLFSPAYDLP 757
            L+K S AI   LE++FYR G  VA+HP   I GA +  AL  LG   F +E    KLWIP +S FA + +WL  NFP P   S  +   EN+LVPEV+Q +++IH+ V ++    G+ W DLC+KLP+                 ++ LPS+ V    E  +   E+ FFG   D+ D+      F  + S   V   +DPS+    E YC +V ++  AC+E++  E+++          + IHNL +++++  +N    S IF R+ +++  LGG I  N  G I+GA+A  +++    N + +   K  +     +  D  T  +E+   ++  +     P G++      RS+GDI+   I++D + L  G+ +++VY+  M+GRFN VEQR FL   GIT  G A++V+ G+ ++  L YGP++ ++P +LLG+G+DDMFVI+Q ++N       K   + +PER+G  +KHAGV IT+TS+TD+  F VG +T        ++P L+S+ +Y A GI A ++LQ TFFVA  A+DQKR++D+R+G   W+ + + ++ P++ SQ+S  +  F+    + +  PI K  V+L TL F  +  +G  LL+ EF  V FLPDDS +  + +     +PG G    +    ++            + F + +D I+SF++WY +F+ Y N +F      IP+ P+  + F + + +FL+SP G++H   F+     +CG + P     T+  V  P    K    A  +P
Sbjct:   18 LEKVSLAIQGSLENFFYRWGVLVATHPFKFIFGAFMFTALLALGFINFTQENRPDKLWIPRDSKFASDTEWLRNNFPSPLRESFAIFTAENVLVPEVMQAIFEIHERVGRISILDGKKWEDLCFKLPITKFNEASKYRRRRRRSVATTLPSREVLMTSENRTAREEDTFFGHAFDDFDNAFNEDFFNEVSSPHVVD--WDPSIELPVETYCSMVNSLDEACWEVNPTELWSFN-------RSLIHNLTKDDIVQVMNYQLTSPIFGRKVNYTAELGGKITHNEKGEIIGAEAIRLKFVVAINQTAVDLGKSMSDFSTGETADEGTWLWEKAWTELMQNASKSLPRGIQVYYANGRSWGDITESLIFEDGMMLVVGFLVMFVYISIMIGRFNMVEQRPFLSIAGITCVGFAVIVAYGIGAMCFLLYGPVNKILPFMLLGIGIDDMFVIMQCWSNLYPSCLTKKCKQSIPERMGMTMKHAGVSITVTSLTDVAAFGVGAST--------IMPGLQSFCIYAAIGIIAVFLLQATFFVACFAVDQKRLEDRRNGFFPWIKYSESEFTPSEWSQKSYYQSVFEWFFFKIILTPIGKTIVILMTLGFLGVNIYGNLLLKQEFDPVWFLPDDSHLALFIKTRENNYPGTGNPAFIVAENVNWTASFESYDKFSEMFKS-SDEIYSFENWYDDFKIYANQHF---GTDIPNKPLDPKLFQKYMGKFLYSPRGSKHRGFFKLEGNFSCGNNLP-----TIKMVMMPFRFRKFAGAAEHIP 763          

HSP 2 Score: 217.238 bits (552), Expect = 1.285e-53
Identity = 107/242 (44.21%), Postives = 157/242 (64.88%), Query Frame = 0

Query:  819 FSCVDPYTTLYPPMSTMEFVHRKFNSSTQHIPAMNWVKDSVV------DAGFPGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTSNEKPPS 1054
            FSC +   T+   M  M F  RKF  + +HIP M+ +K+ V       + G  G     + +   WE DE+I++EL++N+  + I V  TT LL+AN   C    LCV+ +L++VGG M+FWGLTID+++  +I+++VGLCVD++AH+A  FM  SGS+  R    +R IGPAV NGG ST +A+L+L NS+SH F S+FK+F ++I FGLFHGL+FLPV+L+L+G +P+        +P S
Sbjct:  734 FSCGNNLPTI--KMVMMPFRFRKFAGAAEHIPGMHHIKNIVASVNLTYNTGKHGRMIPWSPAMVTWETDELITEELYKNVLFSFISVLGTTFLLLANIRVCFFCGLCVIFTLINVGGMMHFWGLTIDMISFIDIVLAVGLCVDYAAHIALAFMSCSGSRAGRAEYAVRSIGPAVLNGGFSTFLAILLLANSQSHAFKSFFKIFTVVICFGLFHGLVFLPVLLTLIGPQPFSNTQDGKNEPGS 973          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1061478937|gb|ODM96499.1| (Niemann-Pick C1 protein [Orchesella cincta])

HSP 1 Score: 429.483 bits (1103), Expect = 1.685e-125
Identity = 259/737 (35.14%), Postives = 406/737 (55.09%), Query Frame = 0

Query:   12 LQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFP-PDLSSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFG-------DFGDE---SDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGG-IQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQQPV-DVTTLEFEQLLQISLDDKCG-YPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVH-GDQWKPNKLSQRSLMEEAFDA-LGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKV 732
            LQK S AI+  +E +FYR G  VA++P   I   L+  A+CGLGL  F  E    KLWIP +S FAHN +WL +NFP P   S ++   +N+L P +++ + +IH++V  +     + W D+C++LP+   +      +      + S +  + FFG       +F  E   SD      +  G  ++FDP +    + YC I++ +  AC+E++ LE+++            + +L +EE+I   NT   S IF R  +++  LGG I+RNS G I+ A++  ++WF   N SE++  +    M    V D T L +E      +D      P+G+       RS+GDIS G I+DD + L  G+F++++YV +MLG+FN VEQR          S ++ ++S G+ S   L YGP++ ++P LLLG+GVDDMFVIVQ +NN   +  +K     +PER+G ALKHAGV IT+TS+TDI  F VG +T        +LP L+S+ +Y A GI A ++LQ TFFVAW A+DQKR++++R+  CC + +  D++ PN  SQRS  +  FD    ++L  P+ K  V+  TL   A   +G T L  EF  + FLP+ S + Q+ +     +PG G  G +   +++     P+++  +  F    + I+ FD+WY +F+ Y N+ F      IP+  ++   F   + +FL+SP G++H   F+ A   +CG   P V
Sbjct:   27 LQKISLAITGTMERFFYRWGFIVATYPYRFISVCLLFTAICGLGLVNFTLENRPDKLWIPRDSAFAHNTEWLRDNFPSPVRQSHVIITADNVLDPGIMKMILEIHESVKNISVFDNKKWEDICFRLPITKFDLERRRRRRRSPTNS-SHHEYDAFFGSAFSGFDEFDSEFFTSDASTNNTTKGGDVRSFDPMLSLPEDIYCSIIDNLDDACWEINPLELWSYN-------RTEVESLTKEEIIQVFNTRLISPIFGRVVNYTADLGGNIERNSLGQIIKAESISLKWFVAINQSEVRAGRAVPDMGTGEVADETGLAWETEFIYLMDSITKQMPDGVNLYFATGRSFGDISQGLIFDDGLMLVAGFFIMFLYVSTMLGKFNLVEQRLMANKNRHCVSIISFIISYGVGSACFLVYGPVNKILPFLLLGIGVDDMFVIVQCWNNLFPDCLSKPNKLSIPERMGMALKHAGVSITVTSLTDIAAFGVGAST--------ILPGLQSFCVYAAIGILAIFLLQSTFFVAWFALDQKRVEERRNAYCCCIQYRDDKFIPNSYSQRSYAQIFFDEYFSKYLFTPVAKTLVITVTLGCLATTIYGNTQLRQEFDPIWFLPEGSHLSQFLEKREVKYPGIGFPGFVVAHQVNWTASFPQVEQFMHDFQGSNN-IYEFDNWYEDFKEYSNENF---GTKIPEKALAPSKFKRNMGKFLYSPAGSKHRAYFKVAGNFSCGDKLPPV 743          

HSP 2 Score: 212.231 bits (539), Expect = 4.560e-52
Identity = 108/252 (42.86%), Postives = 160/252 (63.49%), Query Frame = 0

Query:  805 RVIHKMTGKTCLLTFSCVDPYTTLYPP--MSTMEFVHRKFNSSTQHIPAMNWVKDSVVDAGFP------GITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIADTS 1048
            R   K+ G      FSC D      PP  M  M F  + F+   +HIP M+ VK  V +A         G  FA +  +  WE DE+I+ EL+R++ L++ CV IT   L+AN   C++  +CV+ ++++VGG MYFW LTI++V+  +I++++GLCVD+++H+   FM  SGS+N R +  LR IGPAV NGG ST I++ +L  S+S  FIS++K+F +++ FGLFHGL+FLPV+LS+LG  PY  A +S
Sbjct:  725 RAYFKVAGN-----FSCGDKL----PPVQMVLMPFRFKYFHGPEEHIPGMHAVKALVKNANLTFNDNSDGHMFAWSTGFVTWESDELITAELYRSVALSLACVLITIFFLLANLRMCLLCGVCVIFTIINVGGFMYFWDLTINMVSCIDIVLAIGLCVDYASHIGLAFMTCSGSRNSRAKQALRNIGPAVLNGGFSTFISISLLARSQSLAFISFYKIFTIVVGFGLFHGLIFLPVILSILGPDPYPNAFSS 967          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1215270791|gb|OXA57102.1| (Patched domain-containing protein 3 [Folsomia candida])

HSP 1 Score: 428.328 bits (1100), Expect = 3.041e-125
Identity = 265/770 (34.42%), Postives = 414/770 (53.77%), Query Frame = 0

Query:   11 GLQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFP-PDLSSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFG--------------DFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGG-IQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQ--QPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHG-DQWKPNKLSQRSLMEEAFD-ALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTVVDVEFPICLSKLFSPAYDLPWL 759
             LQK S AI+  +E +FYR G +VA+HP   I   L+  ++CGLG+F F  E    KLWIP NS+FA N +W+   FP P  +S  +   +N+LVPEV++ +  IH+ V Q+  +GG+ W  LCYKLP+    S     + + +    S +T     G              +F  E+       S +G  + F    V   E YC IV  +  AC+E+S  E+++         ++ I NL QEE+I  VN    S IF R  +++   GG I RN  G ++ A +  +RW     I++   ++GR   D+  +  D   LE+E + + +  +     P G+ +     +S+ +IS   I DD + L  G+ +++ YV  MLG+FN VEQR  L   G++  GLA +VS GLCSL  + YGP++ ++P LLLG+G+DDMFVI+Q +NN             +PER+G A+KHAGV IT+TS+TD+  F VG +T        ++P L+S+ +Y A GI A ++LQ TFFVA  A+DQKR++D+R+    W+ +  D++ PN  SQ S ++  F+  L ++L  P  KI+V+L TL+   +  +G TLL  EF  + FLP DS + ++ +    Y+PG G    L  ++++  +       +++     +D I++ ++WY +F+ Y N  F   D  IP+ P++   F + + +FL+SP G++H   F+     +CG   P   + TVV    P    K   P   +P L
Sbjct:   35 SLQKISLAITGSMEKFFYRWGFFVATHPLKAISCCLLITSICGLGIFNFTMENRPDKLWIPRNSEFAQNTEWIRATFPSPIRTSFAIVTADNVLVPEVMRVILNIHERVKQIEVTGGKNWESLCYKLPITKFLSKR-KRRSIGQAYLHSNSTTNSHTGLQILSNDFDDAFDDEFFSENSTLKPQTSESGFSQLF----VVPDEFYCSIVNELDAACWEVSPAELWSFD-------KSLIDNLTQEEIIRVVNHELISPIFGRSVNYTAEFGGNIVRNDLGEVISAGSVLLRW--SIGINQTLLQEGRVVTDKTGEVADDDCLEWELKWIDLMKNISKDMPPGINTYFVNSQSWMEISEATILDDGMQLAMGFIIMFFYVGIMLGKFNVVEQRPLLTIAGLSCVGLACVVSYGLCSLCFIVYGPVNKILPFLLLGIGIDDMFVIMQCWNNLFSSPLTNKSKLSIPERMGMAMKHAGVSITVTSLTDVAAFGVGAST--------IMPGLQSFCIYAAVGIIAVFLLQATFFVACFALDQKRVEDRRNAFFLWIKYKEDEFIPNSWSQESYLQTLFNRILAKYLFTPWAKITVILITLSMLGVTMYGNTLLRQEFDPMWFLPSDSYMSKYVEKRETYYPGTGYPSFLVANQVNWSESFQSFDRVLNDL-KSSDAIYNLENWYDDFKEYSNANF---DTNIPNRPLTPSKFQKYMGKFLYSPAGSKHRPYFKSDGNFSCGESLPP--LKTVV---MPFRFRKFNGPQEHIPAL 773          

HSP 2 Score: 147.132 bits (370), Expect = 8.309e-32
Identity = 87/236 (36.86%), Postives = 120/236 (50.85%), Query Frame = 0

Query:  819 FSCVDPYTTLYPPMST--MEFVHRKFNSSTQHIPAMNWVKDSVVDAGF-------PGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIA 1045
            FSC +      PP+ T  M F  RKFN   +HIPA+  VK  +  A         P   F        WE D +I+ EL+RN+ L+++CV +TT LL+AN   C                                         D++ H+A  FM  +GS+  R +  L+ IGPAV NGG ST +A+++L  SKSH F S+FK+F ++IV GLFHGL+FLPV+LS+ G +PY  A
Sbjct:  742 FSCGESL----PPLKTVVMPFRFRKFNGPQEHIPALRKVKSLLKTANMTFNHHESPKF-FVTGTGLVAWETDNIINAELYRNVSLSLLCVLVTTFLLLANFRIC-----------------------------------------DYATHIALAFMTCTGSRESRAKQALKNIGPAVLNGGFSTFLAIILLAGSKSHAFQSFFKIFSVVIVCGLFHGLIFLPVLLSIFGPEPYPFA 931          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228374474|ref|XP_021948457.1| (protein patched homolog 2-like [Folsomia candida])

HSP 1 Score: 427.943 bits (1099), Expect = 1.113e-124
Identity = 265/769 (34.46%), Postives = 414/769 (53.84%), Query Frame = 0

Query:   12 LQKASDAISKGLEDYFYRHGKWVASHPKLTILGALVGVALCGLGLFRFNEEKNMVKLWIPPNSDFAHNNQWLWENFP-PDLSSVILEHEENILVPEVIQQMYQIHKNVLQLRTSGGQGWLDLCYKLPVVDVESSDLPSKPVQKREAPSPNTEEDFFG--------------DFGDESDDFAAIKSGAGVGKAFDPSVVFYPEQYCPIVEAMPMACFEMSILEMFAQGGQFTPKVEATIHNLDQEELITAVNTIGQSGIFLRERDFSKYLGG-IQRNSTGHIVGAKATFIRWFGRANISEIQNEKGRNGMDQ--QPVDVTTLEFE-QLLQISLDDKCGYPEGLKSQANVQRSYGDISNGAIWDDVIFLTCGYFMIYVYVQSMLGRFNRVEQRAFLGAMGITSSGLAILVSMGLCSLMGLDYGPMHSLIPCLLLGLGVDDMFVIVQSFNNSEGEDKAKGIIKDLPERIGQALKHAGVGITITSVTDIVVFAVGGTTTIDECRLQVLPSLRSYSLYTATGIAATYVLQVTFFVAWLAIDQKRIDDKRDGACCWVVHG-DQWKPNKLSQRSLMEEAFD-ALGRFLAYPIVKISVLLTTLAFFALGCWGTTLLEVEFRYVEFLPDDSSIYQWFQLHTGYFPGDGEMGTLYFSEMDLYKELPKIKNLVDKFTNETDIIFSFDSWYPEFESYLNDYFLQPDEAIPDHPISEEFFNEKLTQFLFSPIGAQHMNKFQFAEPIACGTDAPKVLVSTVVDVEFPICLSKLFSPAYDLPWL 759
            LQK S AI+  +E +FYR G +VA+HP   I   L+  ++CGLG+F F  E    KLWIP NS+FA N +W+   FP P  +S  +   +N+LVPEV++ +  IH+ V Q+  +GG+ W  LCYKLP+    S     + + +    S +T     G              +F  E+       S +G  + F    V   E YC IV  +  AC+E+S  E+++         ++ I NL QEE+I  VN    S IF R  +++   GG I RN  G ++ A +  +RW     I++   ++GR   D+  +  D   LE+E + + +  +     P G+ +     +S+ +IS   I DD + L  G+ +++ YV  MLG+FN VEQR  L   G++  GLA +VS GLCSL  + YGP++ ++P LLLG+G+DDMFVI+Q +NN             +PER+G A+KHAGV IT+TS+TD+  F VG +T        ++P L+S+ +Y A GI A ++LQ TFFVA  A+DQKR++D+R+    W+ +  D++ PN  SQ S ++  F+  L ++L  P  KI+V+L TL+   +  +G TLL  EF  + FLP DS + ++ +    Y+PG G    L  ++++  +       +++     +D I++ ++WY +F+ Y N  F   D  IP+ P++   F + + +FL+SP G++H   F+     +CG   P   + TVV    P    K   P   +P L
Sbjct:   36 LQKISLAITGSMEKFFYRWGFFVATHPLKAISCCLLITSICGLGIFNFTMENRPDKLWIPRNSEFAQNTEWIRATFPSPIRTSFAIVTADNVLVPEVMRVILNIHERVKQIEVTGGKNWESLCYKLPITKFLSKR-KRRSIGQAYLHSNSTTNSHTGLQILSNDFDDAFDDEFFSENSTLKPQTSESGFSQLF----VVPDEFYCSIVNELDAACWEVSPAELWSFD-------KSLIDNLTQEEIIRVVNHELISPIFGRSVNYTAEFGGNIVRNDLGEVISAGSVLLRW--SIGINQTLLQEGRVVTDKTGEVADDDCLEWELKWIDLMKNISKDMPPGINTYFVNSQSWMEISEATILDDGMQLAMGFIIMFFYVGIMLGKFNVVEQRPLLTIAGLSCVGLACVVSYGLCSLCFIVYGPVNKILPFLLLGIGIDDMFVIMQCWNNLFSSPLTNKSKLSIPERMGMAMKHAGVSITVTSLTDVAAFGVGAST--------IMPGLQSFCIYAAVGIIAVFLLQATFFVACFALDQKRVEDRRNAFFLWIKYKEDEFIPNSWSQESYLQTLFNRILAKYLFTPWAKITVILITLSMLGVTMYGNTLLRQEFDPMWFLPSDSYMSKYVEKRETYYPGTGYPSFLVANQVNWSESFQSFDRVLNDL-KSSDAIYNLENWYDDFKEYSNANF---DTNIPNRPLTPSKFQKYMGKFLYSPAGSKHRPYFKSDGNFSCGESLPP--LKTVV---MPFRFRKFNGPQEHIPAL 773          

HSP 2 Score: 216.468 bits (550), Expect = 2.114e-53
Identity = 107/236 (45.34%), Postives = 154/236 (65.25%), Query Frame = 0

Query:  819 FSCVDPYTTLYPPMST--MEFVHRKFNSSTQHIPAMNWVKDSVVDAGF-------PGITFAMAQSYSRWEIDEVISQELFRNLFLAIICVSITTMLLIANTTSCIIVILCVVMSLVDVGGAMYFWGLTIDIVASTNIIISVGLCVDFSAHVAHYFMLQSGSKNERMRSTLRKIGPAVFNGGISTLIAVLMLMNSKSHVFISYFKVFFLMIVFGLFHGLLFLPVVLSLLGSKPYKIA 1045
            FSC +      PP+ T  M F  RKFN   +HIPA+  VK  +  A         P   F        WE D +I+ EL+RN+ L+++CV +TT LL+AN   C    +CVV++L++VGG M+FWGL+ID V++ +I++++GLCVD++ H+A  FM  +GS+  R +  L+ IGPAV NGG ST +A+++L  SKSH F S+FK+F ++IV GLFHGL+FLPV+LS+ G +PY  A
Sbjct:  742 FSCGESL----PPLKTVVMPFRFRKFNGPQEHIPALRKVKSLLKTANMTFNHHESPKF-FVTGTGLVAWETDNIINAELYRNVSLSLLCVLVTTFLLLANFRICAFCGICVVLTLINVGGFMHFWGLSIDTVSTIDIVLAIGLCVDYATHIALAFMTCTGSRESRAKQALKNIGPAVLNGGFSTFLAIILLAGSKSHAFQSFFKIFSVVIVCGLFHGLIFLPVLLSIFGPEPYPFA 972