hypothetical protein DAPPUDRAFT_333049, maker-scaffold1237_size53912-snap-gene-0.7 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000008334 (supercontig:LSalAtl2s:LSalAtl2s508:27226:174463:1 gene:EMLSAG00000008334 transcript:EMLSAT00000008334 description:"maker-LSalAtl2s508-snap-gene-1.26") HSP 1 Score: 803.512 bits (2074), Expect = 0.000e+0 Identity = 495/943 (52.49%), Postives = 610/943 (64.69%), Query Frame = 0
Query: 4 LLELNQAEVIRPGICVISVPDETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAAS---------RNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-------KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAF--GFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMM 927
L L V+RP V VP+ +T G+ PC+ +PVG +KW GPN GITSFDNIG AMLTVFQ TNDA+GN FN I+F+ LI++GSFFMLNL LGVLSGEFAKE+ERVE+R F++L+ QQ+LE+ELNG + K + I E+T S E DDD + FWR E++ RFFIR YAEF+FLG FI E+++R+YALGPR YF ++FN FDC VIV S+ EV+W + + GSFGLS LRALRLLR+FKVTKYWSSLRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQ+FGG+FNF GTP SNFNT ALLTVFQVLTGEDWN+VMY I+S+GG + GM+Y LYF++LTL G+YTLLNVFLAIA D+L Q E + +++++TT + + E + F E A +I + KPILPYSS FILSSSNPIR+ IH + T FD FIM +I++SSIALA+EDPV E S RN +L Y DY FT IF +E LK+IDLGF+LHPG+Y RD WN LD VV+CAL++F G+ TGQ L KSL++LRVLRPLKTI RVPKLKAVFDCVV SLKNVFNILIVY+LFQ IFA++ VQLFNGKFFFC+D+S+ NA+ECQG YF + N P + R W+++ FH+DN A++TLFA+QT ILQ+SMA+TYE+ GP P +R+EMSIFYIVFF+VFPFFFVNIFVALII+TF E GEAELQD +DKNQK+CIDFAIQARPLE Y+P+ D F Y WR++ S+PFE FIM+LI+ NTILLM+
Sbjct: 206 LSTLRTFRVLRPLKLVSGVPNRIITGGDSGNPCYRGSSKHSPVG------------KKWVGPNNGITSFDNIGLAMLTVFQ-------------TNDALGNLFNWIFFIPLIIVGSFFMLNLFLGVLSGEFAKEKERVESRVGFMQLKEQQKLEKELNG----VQKTHKFIKENEQT---------------------------SFKKEFDDDSN-------------------------FWRSERQFRFFIR--------------------------------------LYAEFIFLGCFILEILIRLYALGPRTYFSTTFNVFDCFVIVGSIVEVIWVHFKDKGGSFGLSALRALRLLRVFKVTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFSTGTPTSNFNTITSALLTVFQVLTGEDWNQVMYTAIDSKGGRKGGGMVYSLYFVMLTLCGDYTLLNVFLAIACDSL--DQAAALTEAEEAEKERHKTTLSDDIFLDMEVHDDKENKKYFDPIKAAEIAFEKAAEAAAKAATQEITIDQEENISIKPILPYSSFFILSSSNPIRVSIHWLXTKKVFDCFIMCVIVLSSIALASEDPVDENSIRNKLLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLDITVVSCALISFYHTLAGTPTGQKL---KSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNVFNILIVYMLFQIIFAIVGVQLFNGKFFFCSDESRTNADECQGYYFHYEVPNETPKIVERTWERRSFHYDNCFRAIITLFAIQT------ILQDSMASTYENEGPKPWYRVEMSIFYIVFFVVFPFFFVNIFVALIIVTFNELGEAELQD-NMDKNQKSCIDFAIQARPLELYVPQRTDGFYYHAWRLITSSPFENFIMVLIICNTILLML 1017 HSP 2 Score: 83.9593 bits (206), Expect = 1.120e-16 Identity = 62/176 (35.23%), Postives = 86/176 (48.86%), Query Frame = 0
Query: 559 LPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCA-------------------------------LVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVP 701
LP SS+F LS NPIR + V + F+ I+ II + I +A + P +++S+ + L + VF GIF +EM KI+ GFILH GSY R+ WNI+D +VV L+ + +TG +LST LR RVLRPLK + VP
Sbjct: 54 LP-SSLFFLSGKNPIRRAVIWFVRWIVFEWIIIFTIIANCIVMALDQHLPHKDKSKLSVQLEELEPVFMGIFTMEMCFKIVADGFILHHGSYLRNPWNIMDFIVVISGSIYVHPNKSFIHISHYNDVIYYFYSTSFLPYLIPKSDKKENTGPDLST---LRTFRVLRPLKLVSGVP 225
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000009889 (supercontig:LSalAtl2s:LSalAtl2s643:800:51309:-1 gene:EMLSAG00000009889 transcript:EMLSAT00000009889 description:"maker-LSalAtl2s643-snap-gene-0.8") HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0 Identity = 443/706 (62.75%), Postives = 489/706 (69.26%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRS--TETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQ 728
VT+G+ VPCHSDDP AP GA+TC N+SICLE WEGPNYGITSFDNIGF +L+ EFAKERERVENRQAFL+LRRQQQLERELNGYVDWICKA E EE +HIMEARKRAANKR+K++ LGK RS TE DDDDDE DEG+SQASA RTRLRN GGC Q FWRCEKRVRF+IR TVKQQWFYWFVI+LVF NT+CVAVEHY+QP+WLT+ LYYAEFVFLGLF+ EM V+ Y L P IV++ F ++ ++ AG F+V + S +SL SMRSIISLLFLLFLFILIFALLGMQLFGGAFNF DGTPPSNFN+FAI+LLTVFQ+LTGEDWNEVMYQGIESQGGHRSGM+Y LYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAEN++ R F+HG IVTFPFFDTFIM+IIIMSSIALAAEDPVQE S RNH+LTYFDYVFTGIFAVEM+LK+IDLG I HPGSY RDVWN++DA+VV CA ++F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQ
Sbjct: 207 VTDGSLKVPCHSDDPLTAPAGAHTCEPNVSICLENWEGPNYGITSFDNIGF--------------------------------------------------AMLTVEFAKERERVENRQAFLRLRRQQQLERELNGYVDWICKAAEERTTEEER-----NHIMEARKRAANKRRKIKVLGKGRSIETEDDDDDDEVDEGISQASAIRTRLRNHGGC-QPFWRCEKRVRFYIRHTVKQQWFYWFVIVLVFLNTLCVAVEHYNQPEWLTELLYYAEFVFLGLFLAEM-VQGYILNPH-----------SIVLIVWSFVPLF---LKYAGPN-------------FQVMEDHS------VSL--SMRSIISLLFLLFLFILIFALLGMQLFGGAFNFSDGTPPSNFNSFAISLLTVFQILTGEDWNEVMYQGIESQGGHRSGMVYSLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENDELVANAGQIR------LFVHG-------------------------------------------------IVTFPFFDTFIMIIIIMSSIALAAEDPVQENSRRNHILTYFDYVFTGIFAVEMILKVIDLGLIAHPGSYLRDVWNLMDALVVICASISFIFDMTGSSKGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVTNILIVYILFQ 765 HSP 2 Score: 91.6633 bits (226), Expect = 4.525e-19 Identity = 57/173 (32.95%), Postives = 88/173 (50.87%), Query Frame = 0
Query: 594 IIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGS-----------------------STGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNG 741
++ + I LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R+ WNI+D +VV +V FG + S G +L T LR +RVLRPLK + VP L+ V ++ +L + I ++ + IFA+I ++ ++G
Sbjct: 22 LLTNCIVLALEEHLPNGDRTIFAQKLEKTEPYFLGIFTVEAALKILALGFVLHRGSYLRNAWNIMDFIVVVTGMVTFGAEASIPFDLTIVRSFRESEASSSSIPSEEGVDLRT---LRAIRVLRPLKLVSGVPSLQVVLKSILKALAPLMQIALLVMFAILIFAIIGLEFYSG 191
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000005510 (supercontig:LSalAtl2s:LSalAtl2s297:203090:236595:1 gene:EMLSAG00000005510 transcript:EMLSAT00000005510 description:"maker-LSalAtl2s297-augustus-gene-2.17") HSP 1 Score: 710.679 bits (1833), Expect = 0.000e+0 Identity = 397/621 (63.93%), Postives = 482/621 (77.62%), Query Frame = 0
Query: 260 KQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGG-HRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLS-ELEMELRNGRSA----YDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNR-PPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 873
KQQW++W VIILVF NT VAVEHY+QPQWLT FLYYAEF+FLG F+ EM +R+YALGP IYF S+FNRFDC+VI S+FEV+W N AGSFGLS LRALRLLR+FKVTKYWS LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG+FNF D TP SNFNT ALLTVFQVLT EDWN VMY I SQGG H G+IY +YF++LTL GNY LLNVFLAIA D+L A ELTAAE + + E +++L+ + +L EL + G DD+ V +PILPYSS+FIL+S+NP R+ +H VT PFFD FIM++I++SS +LAAEDPV+E RN +L YFDYVFT IFA+E +LKI+DLG HPGSY RD+WN+LD++VV+CAL++F FQG+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNVFNILIVY+LF IFA++ VQLFNGKFF+C+D SK NAEECQGEYFVF + P V R+W + F++DN AA++TLFAVQT+EGW +LQNSM++T+ED GP P F EM+IFYIV+F+VFPFFFVNIFVAL+I+TF E GEAEL D +IDKNQ
Sbjct: 3 KQQWWFWLVIILVFLNTCTVAVEHYNQPQWLTIFLYYAEFLFLGAFVFEMCIRLYALGPFIYFSSAFNRFDCVVITGSIFEVLWVNLQPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFPDETPTSNFNTIINALLTVFQVLTEEDWNNVMYXAIVSQGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSLDQAAELTAAEEAEKERQEEEQERQRLREQQELKAIGADGQLGDELRGDEEEGGEPPLPDMDDEDQV-RPILPYSSLFILTSTNPFRVGLHWFVTKPFFDGFIMIVILLSSASLAAEDPVEEHHPRNLLLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALISFYFQGTPMGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNSMSSTFEDEGPFPWFSTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNELGEAELTD-DIDKNQ 618
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000004952 (supercontig:LSalAtl2s:LSalAtl2s260:1045447:1062229:1 gene:EMLSAG00000004952 transcript:EMLSAT00000004952 description:"maker-LSalAtl2s260-snap-gene-10.24") HSP 1 Score: 452.981 bits (1164), Expect = 1.538e-139 Identity = 323/947 (34.11%), Postives = 449/947 (47.41%), Query Frame = 0
Query: 39 DDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRS--GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL------------------------RNGRSAYDD-------------------------------DIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMM 927
DD + G Y C + +C E GPN+GIT+FDN +MLTVFQC+T+EG+ + W +A GN G EF+KER + +R + K + +Q+ ++ GY+DW+ AE ++ L RS E D+ + + L T + + ++ R R VK Q +W I +F C FV L + ++ R + + P G+SVLR +RLLR FKVT+YW+SL+NL+ SL++S+ +I SL+ LLFLF+ I+ALLG QLFGG F ++LTGEDWN VMY GI + GG ++ GM LYFI L + GNY LLNVFLAIAVDNLA + E + + A ++ + GTQ E E L NG Y+D D + PI P+SS+FI S +N R+ + +F FI+V I++SS +LAAEDP+ +S RN V+ Y G + H G+Y R NILD VVV+ ++++ F+ S+ +S +K LRVLRVLRPL+ + R LK V V+ S+ + NI++V +L QF+FAVI VQLF GKF+ C+D SK EECQG+Y F + P V R W FH+DNVA MLTLF V T EGWP IL S+ + ED GP+P+ R M IFYIV+ I+ FF VNIFV +I+TFQ++GE+ + E+DKNQ+ CID+ + A+P Y+P D +Y+IW S+ FEY + I+LNTI L M
Sbjct: 241 DDDPRLCGGNYQCET--GVCREYIPGPNFGITNFDNFILSMLTVFQCITLEGFMT--QW--EAPGN-------------GRIL-----------EFSKERTKTTSRGIYQKAKMLEQMNDDMKGYMDWLLIAE-----------------------------XLEPLQYKRSAEASSDEKKGIDPLQLKQTLHT------SKLAKLAKINRKFRRACRKAVKSQVMFWNYFISLFNRFDC--------------------FVVLSSILELVLTRTHIMPP----------------------------------LGMSVLRCIRLLRAFKVTRYWTSLKNLLKSLINSIEAIFSLIVLLFLFLGIYALLGSQLFGGKF--------------------XERILTGEDWNAVMYDGILAYGGPQTVLGMFASLYFITLFIIGNYILLNVFLAIAVDNLAGDDDDEEXETPVVKDPEPAFAPAQQDPSRPPP--PGTQIPHEREDLLMMIDNHHLYNYDPGYEDEYNPYLNSNGYDHYNDYNQMVNNNGPIKSLEEKLEEEKANEEAFLEDDDNKKDPIPPFSSLFIFSPTNRFRVLCYNFCNHAYFANFILVCIMVSSASLAAEDPLHSKSPRNIVIAY--------------------GAVGHKGAYARYPANILDLVVVSVSIISIAFRDSAGA--ISVLKILRVLRVLRPLRAVNRAKGLKLVIXAVIVSVSTIQNIVLVTVLLQFLFAVIGVQLFKGKFYSCSDSSKMTEEECQGQYVDFKKSDWAQPTVLEREWSLNDFHYDNVAKGMLTLFVVSTFEGWPGILYTSLDSHAEDTGPLPISRPSMFIFYIVYIIIIAFFMVNIFVGFVIVTFQKEGESPFANCELDKNQRNCIDYTLNAKPARLYLPS--DPVQYRIWAWAXSSKFEYVVFGAILLNTISLAM 1022 HSP 2 Score: 112.464 bits (280), Expect = 2.469e-25 Identity = 75/258 (29.07%), Postives = 134/258 (51.94%), Query Frame = 0
Query: 251 VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEV-MWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPS--NFNTFAIALLTVFQVLTGEDWNEVMYQGIES----------QGGHRSG---MIYCLYFIILTLFGNYTLLNVFLAIAVDN 492
V++ I + F + V + NT+ +A+ Y+QPQ +DFL ++F F E V++M A P+ YF +N FDCI+++ S ++ M + G ++ R R LR+ K+ S+R L+ + + S +++ ++ L+ L I+ ++GMQ+FG +D + S NF F AL+ +F+ TGE W ++M I S + G+ +G +I YFI + ++ +LN+F+A+ +DN
Sbjct: 992 VQYRIWAWAXSSKFEYVVFGAILLNTISLAMSFYNQPQIYSDFLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINEDTSIHSNNNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDN 1249 HSP 3 Score: 98.2117 bits (243), Expect = 6.526e-21 Identity = 73/253 (28.85%), Postives = 123/253 (48.62%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQ--EQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQG---SSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDK-----SKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGW 804
S+++ S NPIR+ +V + F+ FI+ I+ + ++LA P + ++ N L + +F IF E V+KI+ G H +Y R+ WN +D ++V GF SS +K+LR RVLRPL+ + VP L+ V + ++ ++ +F+I ++ L I+++I +Q+F G C D + C G Y + R + P +FDN +MLT+F T EG+
Sbjct: 47 SIYLFSLKNPIRLKCITVVEWKAFEFFILATILATCVSLALHKPYPNGDTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFID-----FSIVMIGFMDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMFQGLMHKACFDNITGVLIDDDPRLCGGNYQCETGVCREYIPGPNFGIT---NFDNFILSMLTVFQCITLEGF 291 HSP 4 Score: 63.929 bits (154), Expect = 2.062e-10 Identity = 77/329 (23.40%), Postives = 134/329 (40.73%), Query Frame = 0
Query: 570 SNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLST-IKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAA--------TYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQE-------QGEAELQD-----GEIDKNQKACI 877
S+P++ I F+ + I++++I+LA Q Q + L +Y+FT FA+E V+K+ P Y D WN D ++V + + G +G I R+ R LR +K + + ++ + + S + + ++++ L FI+ V+ +Q+F GK D S + S+ N F N +A++ LF T E W I+ + + + + E P I ++I FFI F +N+FVA+I+ F G L + E D + K CI
Sbjct: 989 SDPVQYRIWAWAXSSKFEYVVFGAILLNTISLAMSFYNQPQIYSD-FLDLLNYIFTIFFALEFVIKMA----AFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVF-GKIAINEDTS------------IHSNNN----------------FQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDNFDYLTRDWSILGAHHLDEFVNLWSEYDPDAKGCI 1283
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000010843 (supercontig:LSalAtl2s:LSalAtl2s721:13130:108134:1 gene:EMLSAG00000010843 transcript:EMLSAT00000010843 description:"maker-LSalAtl2s721-augustus-gene-1.27") HSP 1 Score: 438.343 bits (1126), Expect = 2.411e-132 Identity = 266/636 (41.82%), Postives = 386/636 (60.69%), Query Frame = 0
Query: 338 VFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVT-KYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-----NFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRS-GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENED-----------------QNETTAASRNMEKQLQFMHGTQK-------------------LSELEMELRNG-RSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
+F +T +E F L + + F+ + +YW L+NL+ SL++S+ +I+SLL LLFLF+ IFALLG Q+FGG + + ++ +NF++F+ +LL+VFQ+LTGEDWN VMY GI++ GG S G++ LYFIIL + GNY LLNVFLAIAVDNLA+A L E++AE E+ N + L + + + E++ +N R + + PI S+ +I +N RI H I +F ++V I++SS LAAEDP+ SERN +L YFDY FT IF +E+ LK+I GF+LHPG++CR +N+LD +VV +L++F F + +S +K LRVLRVLRPL+ I R LK V CV+ + K + NI++V LL QF+FAVI VQLF GKFF C+DKSK AE C+G++ V+ + P+V R+W++ FH+DNV +LTLF V T EGWP IL S+ + ED GP+ +R +++FY ++ I+ FF VNIFV +I+TFQ +GE+ ++ E+DKNQ+ CI+FA+ A+P+ RY+PK + +YK+W V S FEY I +I++NT+ L MK
Sbjct: 749 IFGGKFTKSLESRSHFDTFPQSCLTVFQGFEDSERYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAFGGVNSIGIVSSLYFIILFICGNYILLNVFLAIAVDNLADADAL---EDEAEKEEGAGPPEDGGVEGVDILGANSHCGGDTELRLSLDQEYSLEDYDQYAEDDEIVGDDDDEEDEGQFELDDKNTLRMDSESPFYKVVPIPQESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFSSGA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCSDKSKMTAETCRGKFIVYKDGDLNQPIVEERIWERNDFHYDNVGKGLLTLFVVSTFEGWPGILYVSIDSNAEDVGPIHNYRPIVAVFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPK--NPIQYKLWAFVTSPVFEYTIFAMILINTLSLAMK 1375 HSP 2 Score: 335.88 bits (860), Expect = 2.116e-97 Identity = 201/511 (39.33%), Postives = 283/511 (55.38%), Query Frame = 0
Query: 12 VIRPGICVISVPDETVTEGNKNVPCHSDDPTKAPVGAYTCNSNIS-ICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWIC------------------------KAEEVILAEERTTDEERSHIMEAR-------KRAANKRKKMQKLGKSRSTETD------------DDDDEP-------------DEGLSQAS------AFRTRLRNQ----GGCC----------------QQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQ 439
V+RP V VP E +D+PT G Y C+ +C E WEGPN+GIT+FD+ G +MLTVFQC+T+EGWT +LYW DAMG+S+ IYFV+ +V+G+FF++NL+LGVLSGEF+KERE+ ++R F KLR+++Q+E +L GY++W+ K + V++ ++T ++E R + A R QK + + E D D +++P D +++S +F RL N+ GG + + +R R R VK +W +IILVF NT +A EHY QP WL DF + F+ LF EM ++MY+LG + YF S FNRFDC V+++S+ E++ TN M GLSVLR +RLLR FKVT+YWSS+ NLV SL++S+ SI SLL +LFLFI IF+LLGMQ+FGG F + S+F+TF + LTVFQ
Sbjct: 278 VLRPLRLVSGVPREME---------ETDNPTLCG-GNYKCDEEAGYVCREYWEGPNFGITNFDHFGLSMLTVFQCITLEGWTDVLYWVQDAMGSSWQFIYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQKLRQKEQMEDDLEGYMEWLTVAELIEPSPTDDNIGGLKVDVGKHKGDPVVMGLLKSTMLPLQQVLEGRGDEFSKEREKAKSRGDFQKHRERQQIEEDLRGYLDWITQAEDLENDPSLPPPNLLGAKNKDSSTNRSSKEISRDSFSARLTNEADGKGGALDVDGTGEPSSWFQKKKKALEKLNRRARRVCRKIVKSXAMFWIIIILVFLNTCVLATEHYKQPIWLDDFQEFTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTNENIMP-PLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFT-KSLESRSHFDTFPQSCLTVFQ 776 HSP 3 Score: 120.939 bits (302), Expect = 7.144e-28 Identity = 177/861 (20.56%), Postives = 340/861 (39.49%), Query Frame = 0
Query: 61 KWEGPN-YGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMG--NSF---NSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDE-------ERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH-YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGL-SVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDGTPPS-------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGG-----HRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAAT-------YEDRGPVPLFRIEMSI-FYIVFFIVFPFFFVNIFVALIIITFQ 857
K G N Y +FD+ ++L+VFQ +T E W ++Y A G NS +S+YF+ L + G++ +LN+ L + V+N L + + E D + +++ A + ++ + +E + A + + + DD+ + S F + + + R +F V++ + ++ +A E + L Y ++ F +F E+ +++ + G P + S FN D +V+ S+ +++ +G+ + +LR LR+LR + L+++V ++ + ++I +++ + L +FA++G+QLF G F ++DG +++ LLT+F V T E W ++Y I+S H I +++ I + + ++N+F+ + QNE +A +N E +N R+ + + KP+ Y NPI+ + VT P F+ I +I++++++LA + Q Q+ L + +FT F +E V K+ F +Y D WN D ++V +L+ + G N+ +I R+ RV+R +K + + ++ + S + + + I+ L FI+ V+ +Q+F K P S+ NR +F A+L LF T E W I+ + EDRG + + ++I FF+V F +N+FVA+I+ F
Sbjct: 832 KSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAFGGVNSIGIVSSLYFIILFICGNYILLNVFLAIA----------VDNLADADALEDEAEKEEGAGPPEDGGVEGVDILGANSHCGGDTELRLSLDQEYSLEDYDQYAEDDEIVGDDDDEEDEGQFELDDKNTLRMDSESPFYKVVPIPQESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFS-----SGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCSDKSKMTAETCRGKFIVYKDGDLNQPIVEERIWERNDFHYDNVGKGLLTLFVVSTFEGWPGILYVSIDSNAEDVGPIHNYRPIVAVFYFIYIIIIAFFMVNIFVGFVIVTF-----------------QNEGESAYKNCELD-----------------KNQRNCIEFALNA-KPVRRYIP------KNPIQYKLWAFVTSPVFEYTIFAMILINTLSLAMKFYQQPQT-YTAFLDVLNVIFTVFFTLEFVFKLGAFRF----KNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFG--------KIAPQEG---------SNINR------------NNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 1602 HSP 4 Score: 90.5077 bits (223), Expect = 1.345e-18 Identity = 78/317 (24.61%), Postives = 131/317 (41.32%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALA--AEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDK 871
S++ + NPIR V + F+ I++ I+ + +ALA P ++ +E N +L +Y+F IF E V+KII GF HP +Y R WN+LD +V +++ SS +K+LR RVLRPL+ + VP+ D N C G Y V W+ F +FD+ +MLT+F T EGW +L Q++M ++++ I+++ ++ FF +N+ + ++ F ++ E G+ K
Sbjct: 151 SIYCWTIKNPIRKRFIQFVEWKPFEFLILLTILGNCVALAIYTPFPAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTIL--SSLRIEGFDVKALRAFRVLRPLRLVSGVPREMEETD--------------------------------------------NPTLCGGNY---KCDEEAGYVCREYWEGPNFGITNFDHFGLSMLTVFQCITLEGWTDVLYWVQDAMGSSWQ------------FIYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQK 406 HSP 5 Score: 54.299 bits (129), Expect = 1.861e-7 Identity = 39/154 (25.32%), Postives = 78/154 (50.65%), Query Frame = 0
Query: 590 IMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKF 743
I++++ +++ LA E ++ + + + F +F EM LK+ LGF Y ++N D VV +++ + L + LR +R+LR K + + + +VNS+ ++ ++L++ LF FIF+++ +Q+F GKF
Sbjct: 607 IIILVFLNTCVLATEH-YKQPIWLDDFQEFTNLFFVVLFTFEMFLKMYSLGF----QGYFVSLFNRFDCFVVISSILELILTNENIMPPLG-LSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKF 754
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000003972 (supercontig:LSalAtl2s:LSalAtl2s2133:21116:38912:-1 gene:EMLSAG00000003972 transcript:EMLSAT00000003972 description:"maker-LSalAtl2s2133-snap-gene-0.4") HSP 1 Score: 344.739 bits (883), Expect = 7.342e-110 Identity = 166/225 (73.78%), Postives = 196/225 (87.11%), Query Frame = 0
Query: 656 LDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNR-PPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACI 877
+D +VV+CA+ +F GSS G+NL T+KSLRVLRVLRPLKTIKRVPKLKAVFDCV+NSLKNVFNIL+VY+LF FIFAVIAVQLFNGKFF+C+D SK +AEE GEYF+FS+ N PP V PR+W++Q+FH+DNVA A+LTLFAVQTTEGWPAILQ SMAAT+ED+ P+P FR+ MSIFYIV+FIVFPFFFVNIFVALIIITFQEQGE+EL++ EIDK C+
Sbjct: 1 MDMLVVSCAITSFAMDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAEE--GEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKKSVECV 223 HSP 2 Score: 58.151 bits (139), Expect = 5.869e-9 Identity = 54/208 (25.96%), Postives = 92/208 (44.23%), Query Frame = 0
Query: 347 VEMAGSFG------LSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF------QDGT-------------PPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGG---HRSGM--IYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENE 509
++M GS G + LR LR+LR K K L+ + +++S++++ ++L + LF IFA++ +QLF G F + QD PP +++ A A+LT+F V T E W ++ Q + E Q R GM Y +YFI+ F +N+F+A+ + E E + + +
Sbjct: 15 MDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAEEGEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFF----FVNIFVALIIITFQEQGESELEEAEIDKK 218
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000006853 (supercontig:LSalAtl2s:LSalAtl2s3888:1494:5853:1 gene:EMLSAG00000006853 transcript:EMLSAT00000006853 description:"maker-LSalAtl2s3888-augustus-gene-0.2") HSP 1 Score: 289.271 bits (739), Expect = 2.096e-91 Identity = 159/193 (82.38%), Postives = 176/193 (91.19%), Query Frame = 0
Query: 309 MVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTA 501
M +RM+ALGPRIY+ESSFNRFDC+VI SVFEV+WT+ AGSFG SVLRALRLLRIFKVT YWSSLRNLVISLLSSM+SIISLL LLFLFILIFALLGMQLFGG FNF DGTPPSNFN+F+IA+LTVFQ+LTGEDWNEVMYQGIESQGG ++G+IY LYFI LT+FGNYTLLNVFLAIAVD+L AQE+TA
Sbjct: 1 MFLRMWALGPRIYYESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGFSVLRALRLLRIFKVTAYWSSLRNLVISLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYQGIESQGGAQAGLIYSLYFIFLTMFGNYTLLNVFLAIAVDSLGQAQEMTA 193
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000007884 (supercontig:LSalAtl2s:LSalAtl2s464:432083:573266:-1 gene:EMLSAG00000007884 transcript:EMLSAT00000007884 description:"maker-LSalAtl2s464-augustus-gene-6.21") HSP 1 Score: 163.31 bits (412), Expect = 4.998e-41 Identity = 103/319 (32.29%), Postives = 162/319 (50.78%), Query Frame = 0
Query: 242 QQFWRCEKRVRFF---IRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVM--WTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF----NFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENE-------DQNETTAASRNMEKQLQFMHGTQKLSELEMELRNG 544
+ F C K +R F + V F ++ + NT + +E + QP+ LT + + VF +F EM++++ A G Y FN FD ++++ S E+ + +EM G+ GLSVLR RLLRI K+ ++ +LR ++ +L +M ++ LL LFI IF++LGM LFG F Q NF++ A +TVFQ+LT EDWN V++ G++ R+ LYF+ L FGNY L N+ +AI V+ +N E +++ +E D + S K F + + E E+RNG
Sbjct: 774 EAFLFCSKGIRRFQAALERIVDHNIFQRGILFCILINTFSMGIEFHKQPELLTKIVEVSNLVFSAIFAVEMILKVSAYGIFRYISDGFNVFDGLIVILSSIEIYNGLIHGIEMDGNSGLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKFCDKVGNQRKCDRKNFDSLLWATVTVFQILTQEDWNIVLFSGMQ-----RTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSNQDEKRSSDRNSEYSKDPVGKLDDFSSQINSPIRYKNDMFHGDSLDYPDEEFEIRNG 1087 HSP 2 Score: 146.747 bits (369), Expect = 6.493e-36 Identity = 104/374 (27.81%), Postives = 184/374 (49.20%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDP-VQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV--AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPK-----ERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
++++ ++ IR+ I + +FD +++ I + I LA E P + S +L + +FT IF +EM +K I G I +Y ++ WNI+D +V + V G + + ++ R++R LRPL+ I R P LK V +++SLK + NI+++ F IF ++ VQLF G F++C P+A + + ++ W K++FDN+ A+++LF + + +GW I+ + A D+ P F I++I F ++ FF +N+FV +++ F + AE Q+ E + A I+ R R M K ++ + +VV S F I +I LN I + M+
Sbjct: 1269 TLYVFHPNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYC---EGPHANIVKNKTQCLELSDK-----GNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFH-RCRAE-QEKEERARRAAKRAKKIEER--RRRMMKAPYYIHYSHYRLSVHKVVTSKYFXLAISAVIGLNVITMAME 1630 HSP 3 Score: 108.227 bits (269), Expect = 4.676e-24 Identity = 113/487 (23.20%), Postives = 204/487 (41.89%), Query Frame = 0
Query: 250 RVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTD--FLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFD-CIVIVASVFEVMWTNCVEMAGSFG-LSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPS-----------------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNL--ANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAE---DPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQ---NLSTIKSLRVLRVLR 692
++R + WF + V++ + N + +A+E + P W + L +F +F EM ++ + G YF++ +N D +VIV+ V V + + F L V R +R LR +V L+ +V +LLSS++ I +++ + F +IF +LG+QLF G F + +G + NF+ AL+++F + + + W +MYQG+++ G + +YFI L + +LN+F+ + V+N A++ + + + R M K ++H + R+ +H +VT +F I +I ++ I +A E PV + L +Y FT +F +E +KI LG G Y D WN LD V+V ++V + + N + ++ +RVLR+ R
Sbjct: 1278 KIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRCRAEQEKEERARRAAKRAKKIEERRRRMMKAPYYIHYSH----------------------------------------YRLSVHKVVTSKYFXLAISAVIGLNVITMAMEFYMMPVVLA----YALKVCNYFFTAVFILEAGMKISALGI----GRYLSDKWNQLDVVIVLLSVVGIVLEEIKSDMMPINPTIMRVMRVLRIAR 1716 HSP 4 Score: 84.3445 bits (207), Expect = 1.123e-16 Identity = 65/207 (31.40%), Postives = 107/207 (51.69%), Query Frame = 0
Query: 545 RSAYDDDIFVQKPIL-PYSSMF------ILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQE---QSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNG 741
R Y DD ++ I P +S F L+ SNP+R F I+ P+F+ M +I+ + + L P ++ ++ R +L D V F VEM +K+I +G + G Y + WN LD +V + + + NLST LR +RVLRPL+ I R+P ++ + ++++L + N+L++ FIF +I VQL+ G
Sbjct: 10 RKPYTDDDYMNSDIGDPPASEFAEYSFRFLNLSNPVRKFCIRILVNPWFERLTMTVILFNCVTLGMYHPCEDTDCKTRRCQILKNCDDVIYIYFTVEMFIKMIAMG-VFGKGCYLSETWNRLDCFIVLAGALEYCLKVEKL--NLST---LRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 210 HSP 5 Score: 74.7146 bits (182), Expect = 1.056e-13 Identity = 97/400 (24.25%), Postives = 169/400 (42.25%), Query Frame = 0
Query: 514 TTAASRNMEKQLQFMH------GTQKLSELEMELRNGRSAYDDDIFV----QKPILP--YSSMF--ILSSSNPIRIF---IHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER-NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNL---STIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQD--GEIDKNQKACI-DFAIQARPLERY 889
+TA S + QL F H T L+E E R+ Y + + + + P Y+S+ L S IR F + IV F I+ I++++ ++ E +Q E ++ + VF+ IFAVEM+LK+ G Y D +N+ D ++V + + + G G + S + LR R+LR LK ++ +P L+ ++ ++ NV + +LF FIF+++ + LF KF DK N +C + +FD++ A +T+F + T E W +L + M T +++++ + N+ VA+++ F Q E D E K+ + DF+ Q RY
Sbjct: 710 STALSAXLTAQLVFSHKDMKYCSTYNLAEAIDENNKKRAIYSLNGSMTGEHELRVTPKKYNSIMEAFLFCSKGIRRFQAALERIVDHNIFQRGILFCILINTFSMGIE--FHKQPELLTKIVEVSNLVFSAIFAVEMILKVSAYGIF----RYISDGFNVFDGLIVILSSIEI-YNGLIHGIEMDGNSGLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKF---CDKVG-NQRKC-----------------------DRKNFDSLLWATVTVFQILTQEDWNIVLFSGMQRTSH----------WAALYFVTLMTFGNYVLFNLLVAILVEGFSNQDEKRSSDRNSEYSKDPVGKLDDFSSQINSPIRY 1065 HSP 6 Score: 62.003 bits (149), Expect = 8.052e-10 Identity = 63/218 (28.90%), Postives = 96/218 (44.04%), Query Frame = 0
Query: 205 LGKS-RSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH----YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG---PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG 414
LGKS R TDDD D G AS F +F VR F + WF + ++ FN V + + H D L + V F EM ++M A+G Y ++NR DC +++A E C+++ LS LR +R+LR + S+R LV+ LL ++ + ++L L F IF ++G+QL+ G
Sbjct: 5 LGKSFRKPYTDDDYMNSDIGDPPASEF-------AEYSFRFLNLSNPVRKFCIRILVNPWFERLTMTVILFNCVTLGMYHPCEDTDCKTRRCQILKNCDDVIYIYFTVEMFIKMIAMGVFGKGCYLSETWNRLDCFIVLAGALEY----CLKVE-KLNLSTLRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 210 HSP 7 Score: 55.0694 bits (131), Expect = 1.021e-7 Identity = 31/98 (31.63%), Postives = 55/98 (56.12%), Query Frame = 0
Query: 53 SNISICLEKWEGPNYGIT---SFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMG---------NSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKER 138
N + CLE + N I +FDN+G A++++F + +GW +I+Y DA+G N + IYF++ +++ FF+LN+ +GV+ F + R
Sbjct: 1464 KNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRCR 1561
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000009714 (supercontig:LSalAtl2s:LSalAtl2s627:140867:158129:1 gene:EMLSAG00000009714 transcript:EMLSAT00000009714 description:"maker-LSalAtl2s627-snap-gene-1.23") HSP 1 Score: 133.265 bits (334), Expect = 9.311e-32 Identity = 103/353 (29.18%), Postives = 174/353 (49.29%), Query Frame = 0
Query: 585 FFDTFIMVIIIMSSIALAAEDP-VQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQ-NLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDK---SKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQ---GEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYK--IWRVVVSTPFEYFIMILIVLNTILLMM 927
+F+ ++ +I++SS + ED + + L Y D + T +F +E LK+ +G P Y + W LD V+V +L+ F S G N+ K++R LR LRPL+ + ++ +K V + ++ +L ++FN+L+V ++F IFA+I V LF G F C D K + E + + N W K FDNV A L L V T +GW I+ +++ + ++ P I M +++ F I+ FF +N+ V +II F EQ G + L D + ++QK I +A + R +K + ++ +V + F+ IM I LN + +MM
Sbjct: 1012 YFEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMG----PVMYFTNAWCWLDFVIVAXSLI--NFIASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDSIXGHKFSHEIIPNKTVCLNQTNAQ-------WINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNEQKNKGGSSL-DAFMTEDQKKYIAAMKKASKKKPLKALPRPYWKPQAIVFGIVTNKKFDMIIMGFIGLNMLTMMM 1350 HSP 2 Score: 93.2041 bits (230), Expect = 1.901e-19 Identity = 55/175 (31.43%), Postives = 98/175 (56.00%), Query Frame = 0
Query: 290 LTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF------NFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQG 458
+ + L + F +F E +++ A+ PR +F +N FD ++++ S+ E++ G GLS+LR+ RLLR+FK+ K W SL +++ + +++ ++ +L +L + I IFA++GMQLFG + ++ P NF F + + VF+VL GE W E M+ + G
Sbjct: 660 MNNLLKNGNYFFTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILSLVELL------AXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGSFYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGE-WIESMWACMYVAG 827 HSP 3 Score: 92.4337 bits (228), Expect = 3.220e-19 Identity = 113/547 (20.66%), Postives = 218/547 (39.85%), Query Frame = 0
Query: 230 FRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH--YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSV---LRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN----------FQDGTPPSN-----------------FNTFAIALLTVFQVLTGEDWNEVMYQGIES-----QGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLF 739
F R+ G FW + R ++ ++F V+ L+ ++ + +E ++ L D LYY + + +F E ++++A+GP +YF +++ D +++ S+ + + G+ + + +R LR LR + ++ +V +L+ ++ SI ++L + +F LIFA++G+ LF G F+ F P+ F+ +A L + QV T + W +M I+S Q + + YF+ + G++ LN+ + + +D E +++ ++ + E Q +++ +K S+ + R + KP + + GIVT FD IM I ++ + + D Q+ VL + F IF +EM+LKI L Y + WN+ D +V +L R ++ + +V +L + NI ++ L FIFAV + LF
Sbjct: 979 FVYRIIPLGDQHSPFWEAWTQQRLMAMRLIENEYFEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMGPVMYFTNAWCWLDFVIVAXSLINFI----ASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDSIXGHKFSHEIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKF------------NEQKNKGGSSLDAFMTEDQKKYIAAMKKASKKKPLKALPRPYW-------KP----------------QAIVFGIVTNKKFDMIIMGFIGLNMLTMMM-DHYQQTEGWTFVLDNLNVFFIMIFTLEMLLKIFALR-----QYYFSEPWNLFDFFIVLLSLGGL----------------------------FLRAKGIRTLLFSLVMALPALGNICLLLFLVMFIFAVFGMSLF 1452 HSP 4 Score: 84.7297 bits (208), Expect = 8.352e-17 Identity = 54/179 (30.17%), Postives = 94/179 (52.51%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNG 741
++F L+ +PIR ++T P F+ I+ I+++ I + D + + +FT I+ E +K++ GFILH +Y RD WN LD V+ + + +L + SLR RV R LK++ VP LK + ++ S+KN+F++ I+ L +FA++ +QL+ G
Sbjct: 146 ALFFLTPFHPIRRIAIRVLTHPLFNFIIICTILVNCIVMMLPDTEYXSAS--------EIIFTSIYTYESAVKLLARGFILHNFTYLRDPWNWLDFAVIGMSYITIAI-------DLGSFSSLRTFRVFRALKSVAXVPGLKTIVGAIIYSVKNLFDVTILTLFGLALFALLGLQLYMG 309 HSP 5 Score: 60.8474 bits (146), Expect = 1.661e-9 Identity = 46/136 (33.82%), Postives = 72/136 (52.94%), Query Frame = 0
Query: 612 ERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV---AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF 744
E N++L +Y FT IFA E +K++ + P + D WN D ++V +LV A G G LS ++S R+LRV + K+ K L + + N+L + N+ +V + FIFAV+ +QLF G F+
Sbjct: 659 EMNNLLKNGNYFFTTIFAFESFMKLMAMS----PRYFFADGWNCFDFLIVILSLVELLAXGVNG------LSMLRSFRLLRVFKLAKSWK---SLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLF-GSFY 780 HSP 6 Score: 56.6102 bits (135), Expect = 2.961e-8 Identity = 29/89 (32.58%), Postives = 53/89 (59.55%), Query Frame = 0
Query: 47 GAYTCNSNISICLEKW-EGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEF 134
GA C ++CL + + PN+G T+FDN G+A L F+ +T + W + LY +N ++F+ I +GSF+++NL+L +++ +
Sbjct: 362 GAGKCPEG-TVCLAGFAQNPNHGYTTFDNFGYAYLCAFRLMTQDYWEN-LYQITLRTAGPWNILFFILNIFMGSFYLINLILAIVAMSY 448
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Match: EMLSAG00000010768 (supercontig:LSalAtl2s:LSalAtl2s712:648445:661651:1 gene:EMLSAG00000010768 transcript:EMLSAT00000010768 description:"maker-LSalAtl2s712-snap-gene-6.14") HSP 1 Score: 129.028 bits (323), Expect = 1.917e-30 Identity = 95/339 (28.02%), Postives = 164/339 (48.38%), Query Frame = 0
Query: 574 RIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDP-VQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSK---------PNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE----------AELQDGEIDKNQKACIDFAIQARPLERYMPK 892
R+ + ++ +F+ ++ +I++SS + ED + +L Y D + T +F +E +LK+ +G +++ G + W LD V+V +L+ FG G N+ K++R LR LRPL+ + ++ +K V + +V +L ++FN+L+V L+F IFA+I + F GKF+ C D+ PN C E +W + +FDNV A L LF V T +GW I+ +++ + ++ P I M F++ F + FF +N+ V +II F EQ E Q I +KA ++A P + P+
Sbjct: 1175 RLQVSRLIENKYFEGVVLTLILLSSFVMTLEDIWFDTRPLLVDMLYYLDRILTVVFFLETLLKLFAMGCVMYFG----NAWCWLDFVIVAVSLINFGASLVGLG-NIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKFYKCLDEETGEKFSHEIIPNKTVCLNETGA-------------VWTNARVNFDNVFIAYLALFQVATFKGWMTIMDDAIDSVEKNNQPHREININMYFFFVFFIVFGSFFTLNLLVGVIIDKFNEQKNKGGSSLDAFMTEDQKKYIAAMKKASTKKPLKALPRPNWKPQ 1495 HSP 2 Score: 104.76 bits (260), Expect = 6.846e-23 Identity = 64/201 (31.84%), Postives = 110/201 (54.73%), Query Frame = 0
Query: 267 FVIILVFFNTVCVAVEHYD--------QPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGT--------PPSNFNTFAIALLTVFQVLTGEDWNEVMY 451
F+ I + N + +A++ YD +L+ L + F +F E +++ A+ PR +F +N FD +++V S+ E++ G GLS+LR+ RLLR+FK+ K W SL +++ + ++ ++ +L F+L + I IFA++GMQLFG ++ DG P NF F + + VF+VL GE W E ++
Sbjct: 753 FITICIAVNVIFMALDRYDIEYDANGGMSPFLSSVLTQGNYFFTTIFAVESFIKLVAMSPRYFFSEGWNCFDFLIVVLSLVELL------AEGVSGLSMLRSFRLLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGK--DYYDGVCEKWDCDMPRWNFTDFLHSFMIVFRVLCGE-WIESIW 944 HSP 3 Score: 78.1814 bits (191), Expect = 8.688e-15 Identity = 59/251 (23.51%), Postives = 113/251 (45.02%), Query Frame = 0
Query: 240 CC-----QQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH--YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF----------NFQDGTPPS-----------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIES 456
CC FW+ R R + ++ ++F V+ L+ ++ + +E +D L D LYY + + +F E +++++A+G +YF +++ D +++ S+ + V + +R LR LR + ++ +V +L+ ++ SI ++L + +F LIFA++G+ F G F F P+ NF+ IA L +FQV T + W +M I+S
Sbjct: 1158 CCIGDPDSPFWQLWYRHRLQVSRLIENKYFEGVVLTLILLSSFVMTLEDIWFDTRPLLVDMLYYLDRILTVVFFLETLLKLFAMGCVMYFGNAWCWLDFVIVAVSLIN-FGASLVGLGNIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKFYKCLDEETGEKFSHEIIPNKTVCLNETGAVWTNARVNFDNVFIAYLALFQVATFKGWMTIMDDAIDS 1407 HSP 4 Score: 64.3142 bits (155), Expect = 1.533e-10 Identity = 52/172 (30.23%), Postives = 85/172 (49.42%), Query Frame = 0
Query: 580 IVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER-------NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF 744
IV PF + FI + I ++ I +A + E + VLT +Y FT IFAVE +K++ + P + + WN D ++V +LV +G S LS ++S R+LRV + K+ K L + + N+ + N+ V + FIFAV+ +QLF ++
Sbjct: 744 IVFDPFTELFITICIAVNVIFMALDRYDIEYDANGGMSPFLSSVLTQGNYFFTTIFAVESFIKLV----AMSPRYFFSEGWNCFDFLIVVLSLVELLAEGVS---GLSMLRSFRLLRVFKLAKSWK---SLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGKDYY 905 HSP 5 Score: 62.3882 bits (150), Expect = 5.841e-10 Identity = 49/205 (23.90%), Postives = 104/205 (50.73%), Query Frame = 0
Query: 301 FLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVE--MAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQ-----GGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQE 498
F+ +F EM+++M+AL YF +N FD +V++ S+ + ++ +E L V+R ++ R+ ++ K +R L+ SL+ + +++++ LLFL + IFA+ GM L F++ S F+ + + D E E+ G + +G+ + + ++IL+ ++N+++A+ ++N + A E
Sbjct: 1520 FIVIFTAEMLLKMFALRHH-YFAEPWNLFDFVVVMLSLAGLFLSDLIEKYFVSPTLLRVVRVAKVGRVLRLIKGAKGIRTLLFSLVMAFPALVNICLLLFLVMFIFAVFGMSL------FKNVKIRSGFDDMCTSAGWAEALDAITDDRECDKPSSETGDAGNCGNYMAGVAFMIIYLILSFL---IIVNMYIAVILENYSQANE 1714 HSP 6 Score: 55.0694 bits (131), Expect = 1.003e-7 Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 0
Query: 47 GAYTCNSNISICLEKW-EGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLS 131
GA C + C+E + PNYG TSFDN G A L F+ +T + W + LY ++ I+F+ I +GSF+++NL+L +++
Sbjct: 282 GAGKCPDGTT-CMEGFGPNPNYGYTSFDNFGSAYLCAFRLMTQDFWEN-LYQITLRTAGPWHIIFFMCNIFLGSFYLINLILAIVA 365
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|322518442|sp|C9D7C2.1|CAC1A_APIME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Cacophony protein) HSP 1 Score: 1244.95 bits (3220), Expect = 0.000e+0 Identity = 662/916 (72.27%), Postives = 761/916 (83.08%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL--RNGRSAY----------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++NIS C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQLE EL Y++WICKAEEVILAEERTT+EE+ HI+E RKRA K+KK+ K + + E + DDD+ D LS ++ + G C+QFW EKR R++IR +VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YF+SSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAEN+ E ED+ + ++ +EK++Q + + ++E+ NG+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFVF P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 340 VKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLEHELYCYLNWICKAEEVILAEERTTEEEKKHILEGRKRAEAKKKKLGKSKSTDTEEEEGDDDQDDGELSSST-------KEKGPCKQFWLAEKRFRYWIRKSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFDSGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENEEEEEDKQKQ---AQEIEKEIQSLQNPKDGGAPKVEICPPNGKGGKQSSEEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTQQDCQGQYFVFEDGALLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1243 HSP 2 Score: 168.318 bits (425), Expect = 3.354e-41 Identity = 191/852 (22.42%), Postives = 354/852 (41.55%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNS----FNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFL---KLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH---YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDGT----PPS--------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSM------AATYEDRGPVPLFRIEMSIFYIVFFIVF-PFFFVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G S+YF+ L++ G++ +LN+ L + A +E K ++ Q++E+E+ L + + I + + + +K + +DDD P L +S F N VR V ++F +F+++++ +++ +A E D P+ + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F F+DG P +++ A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ ++ N ++ I IV F+ FIM +I+++++ L + Q + +N L Y + FTG+F VE +LKI G ++ +D WN D + V ++V A + G+N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F G D S + +N +F + ++ LF T E WP I+ + + A + G ++ Y V FI F F +N+FVA+I+ F
Sbjct: 729 TNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENEEEEEDKQKQAQEIEKEIQS------------LQNPKDGGAPKVEICPPNGKGGKQSSEEEK------KQDEDDDTGPKPMLPYSSMFILSPTNP-------------VRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPR--NEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTQQDCQGQYFVFEDGALLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPL----ERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGV----RNFFKDAWNTFDFITVIGSIVDALVIE---FGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF-GNIALDADTS-------------ITKHN---------------NFQSFIQGLMLLFRCATGEAWPNIMLSCVKGRPCDAKAGKQEGGC---GSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1463 HSP 3 Score: 134.035 bits (336), Expect = 1.982e-30 Identity = 98/322 (30.43%), Postives = 158/322 (49.07%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEYFV-FSSYNRPPMVFPR------------LWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P Q+++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 146 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKQDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRDINVIVKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 452 HSP 4 Score: 73.559 bits (179), Expect = 7.399e-12 Identity = 74/308 (24.03%), Postives = 132/308 (42.86%), Query Frame = 0
Query: 248 EKRVRFFIRSTVKQQW--FYWFVIILVFFNTVCVAVEHYDQPQ---WLTDFLYYAEFVFLGLFITEMVVRMYALGPRI----YFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN------------FQDG--TPPSN----------------------------------FNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQE 498
K RF I +W F + V++ + N V +A+E + Q L L E FLG+F E +++ ALG + Y + +N D V+V F ++ +E+ L LRA+R+LR K+ SL+ ++ S++ +M ++ + L+ I+IFA++G++ + G + ++G P N F+ A+LTVFQ +T E W ++Y ++ G + +YFI L + G++ +LN+ L + A +E
Sbjct: 158 RKHTRFII------EWPPFEYAVLLTIIANCVVLALEEHLPKQDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTG-FITAFSQGIEL--DMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRDINVIVKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN----WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 452
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|60391851|sp|P91645.3|CAC1A_DROME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Protein cacophony; AltName: Full=Protein nightblind A; AltName: Full=Protein no-on-transient B; Short=Dmca1A) HSP 1 Score: 1231.85 bits (3186), Expect = 0.000e+0 Identity = 644/925 (69.62%), Postives = 743/925 (80.32%), Query Frame = 0
Query: 23 PDETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFV-----------------QKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGF--QGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
P++ V EG PC++D+ + G++ CN+ S+CLEKWEGPN GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G++FN IYFV LIVIGSFFMLNLVLGVLSGEF+ ER RVE R F K R + + + Y+DWI +AEEVILAEERTT+EE+ HIMEAR+R A KRKK++ LGKS+ST+T++++ E D G +TR + QG C FWR EKR RF+IR TVK QWFYWFVI+LVF NTVCVAVEHY QP +LT+FLYYAEF+FLGLF++EM ++MYALGPRIYFESSFNRFDC+VI S+FEV+W+ GSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLL+SMRSIISLLFLLFLFILIFALLGMQLFGG FN GTP +NFNTF IALLTVFQ+LTGEDWNEVMYQGI SQGG + GMIY +YFI+L LFGNYTLLNVFLAIAVDNLANAQELTAAE + ED+ + + +EK+++ + + + NG A KP+LPYSSMFILS +NPIR H +V P+FD FIMV+I MSSIALAAEDPV+E S RN +L YFDY FTG+F +EM+LKI+DLG ILHPGSY R+ WNI+DAVVV CA V+FGF GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIF+VI VQLFNGKFF+CTD+SK + ECQG YF + P R+W+ + FH+DNVAAAMLTLFAVQT EGWP +LQ+SMAATYEDRGP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF I ARPLERYMPK R++FKYK+WR+VVSTPFEYFIM+LIV NT+LLMMK
Sbjct: 205 PNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFSNERNRVERRMEFQKCRFRAMFQTAMVSYLDWITQAEEVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYG--DDGYLKTRSKPQGSCTG-FWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEV--KGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQL---QELEKEMEALQADG------VHVENGDGAVAPSKSKGKKKEEEKKEEEEVTEGPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMK 1115 HSP 2 Score: 150.984 bits (380), Expect = 1.072e-35 Identity = 192/848 (22.64%), Postives = 354/848 (41.75%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNS----FNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVE---NRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH-YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--------------------QDGTPPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRS---------GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQ-EQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWP----AILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVF-PFFFVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y + G + SIYF+ L++ G++ +LN+ L + A +E + K ++ Q+LE+E+ +A+ V H+ E A K K + E ++ + P L +S F N +R V +F +F+++++ +++ +A E + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S+++++++L + LF IF+++G+QLF G F + +D P +++ A A+LT+F V TGE W +V+ + + R + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + + F G + L E + R+ + ++ RI +V+ PF + FIM++I+ +++ L + Q + E++ L Y + FTG+F+VE VLKII G ++ +D WNI D + V ++V A + N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F + S + +N +F + ++ LF T E WP A L+ ++ P ++ Y V FI F F +N+FVA+I+ F
Sbjct: 602 TNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEA-----LQADGV-------------HV-ENGDGAVAPSKSKGKKKEEEKKEEEEVTEGPKPMLPYSSMFILSPTNP-------------IRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSC--------IDFTIGARPL---ERYMPKNRNTFKYKVW--------------------RI----VVSTPF-EYFIMMLIVFNTLLLMMKYHNQGDMYEKS--LKYINMGFTGMFSVETVLKIIGFGV----KNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENS-------------ITRHN---------------NFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1340 HSP 3 Score: 125.176 bits (313), Expect = 9.436e-28 Identity = 90/321 (28.04%), Postives = 154/321 (47.98%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEYFV--------------FSSYNRPPMVFPRLWQKQK---FHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQ 859
+S+FIL+ NPIR + I+ +P F+ +++ II + + LA E+ P +++ L + F IF VE LKI+ LG +LH SY R++WNI+D VV + F Q G + +++LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C PN +GE F N M + W+ FDN+ AMLT+F T EGW AIL +++ + + I+++ ++ FF +N+ + ++ F +
Sbjct: 16 TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVV---VTGFMTQYPQIGPEVD-LRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEK-WEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFN------------WIYFVPLIVIGSFFMLNLVLGVLSGEFSNE 319
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|46576355|sp|O57483.1|CAC1S_LITCT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=FGalpha1S; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 759.214 bits (1959), Expect = 0.000e+0 Identity = 435/959 (45.36%), Postives = 582/959 (60.69%), Query Frame = 0
Query: 18 CVISVPDETVTEGN-KNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKR-VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQD-GTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAAS---RNMEKQLQFMHGTQ-------------KLSELEMELRNGRSAYDDDIFVQK---------------------------PILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPM-VFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
C D T T N K PC S + C+ N S C W GPN GIT FDN GFAMLTV+QC+TMEGWT +LYW NDA+GN + IYFV+LI++GSFF+LNLVLGVLSGEF KERE+ ++R AF LR QQ ++ +L GY+DWI AE +M+ + ++++ G +ETD + EG+++ AF + WR R +R R VK ++FYW VII++ NTV +A EH+ QP LT A V L LF EM+V++YALG + YF S FNRFD V+ + EVM ++ G+SVLR +RLLRIFK+T+YW+SL NLV SLL+S+RSI SLL LLFLF++IFALLGMQ+FGG F+F+D S F+TF AL+TVFQ+LTGEDW VMY GI + GG SGM C+YFIIL + GNY LLNVFLAIAVDNLA A+ LT+A+ E + + A + + E++L Q K+ E E + + Y F P+ SS FI S +N IR+ H I+ F FI++ I++SSI+LAAEDP+Q +S RN VL+ D VFT IF E+VLK+ G LH GS+CR+ +NILD VV +L++ G + S+ +S +K LRVLRVLRPL+ I R LK V C+ ++K + NI++V L QF+F+ I VQLF GKF+ CTD +K A+EC+G +FV N M PR+W FHFDNV + M++LF + T EGWP +L ++ + ED GP+ +RIE+++F+IV+ I+ FF +NIFV +I+TFQEQGE E +D E+DKNQ+ C+ +A++ARPL RY+PK + +YKIW VV S+ FEY + LI LNTI L M+
Sbjct: 224 CYFKDTDITATVDNEKPAPCSSTGQGRQ------CSINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQMLREQQAMDEDLRGYLDWITHAE----------------VMDPDMEPRDGFSQLEEGG----SETDSLYE--IEGINKFIAFFRQ-----------WRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDSLTKAQDIANEVLLALFTMEMIVKIYALGFQSYFMSLFNRFDSFVVCTGLLEVMLV-ASDIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDLEVRRSTFDTFPQALITVFQILTGEDWTAVMYNGIMAYGGPTYSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLARANPDKTEEEKLLLAKKEQKAKGEGIPTTARLKIDEFESNVNEIKDPYPSADFPGDDEEEEPEIPISPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKIRVLCHRIINATTFTNFILLFILLSSISLAAEDPIQPESFRNKVLSKLDIVFTVIFTTEIVLKMTAYGAFLHKGSFCRNSFNILDLSVVGVSLISMGIESSA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFVAKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHAEDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVIVTFQEQGEQEYKDCELDKNQRQCVQYALKARPLRRYIPK--NPHQYKIWYVVTSSYFEYLMFFLITLNTISLGMQ 1136 HSP 2 Score: 142.124 bits (357), Expect = 5.469e-33 Identity = 182/880 (20.68%), Postives = 358/880 (40.68%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNS-----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQAS--------------AFRTR------LRNQGGCCQQ-----FWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQ-WLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN-------------------FQDGTPPS-------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAF--------GFQGSSTGQNLS--TIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATY-------EDRGPVPLFRIEMS---IFYIVFFIVFPFFFVNIFVALIIITF 856
++FD A++TVFQ +T E WT+++Y A G S IYF+ L V G++ +LN+ L + A+ ++A + R++++L R A T+EE+ ++ A+K K + + + + E + + +E + A + R R L+ + + + ++R + F F+++ + +++ +A E QP+ + L + VF +F TE+V++M A G ++ S SFN D V+ S+ + +E + + +LR LR+LR + L+++V L ++++I +++ + L +F+ +G+QLF G F +DG P +F+ ++++F + T E W +++Y+ I+S G IY ++FI+ + + ++N+F+ + Q + E + + +N + +Q+ + L ++ K NP + I +VT +F+ + +I +++I+L + Q +E +++ + FTGIF VEM LK+ Y D WN+ D ++V +++ G + + S +I R+ RVLR +K + R ++ + + S + + + ++ ++ FI+AVI +Q+F GK D + N + +F A+L LF T E W IL +A +Y D P + S ++I F+++ F +N+FVA+I+ F
Sbjct: 594 STFDTFPQALITVFQILTGEDWTAVMYNGIMAYGGPTYSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLAR-----------------ANPDKTEEEK--LLLAKKEQKAKGEGIPTTARLKIDEFESNVNEIKDPYPSADFPGDDEEEEPEIPISPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKIRVLCHRIINATTFTNFILLFILLSSISLAAEDPIQPESFRNKVLSKLDIVFTVIFTTEIVLKMTAYGAFLHKGSFCRNSFNILDLSVVGVSLISM----GIESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFVAKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHA-EDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVIVTF-----------QEQGEQEYKDCELDKNQRQCVQYALKARPLRR----------------YIPK--------------NPHQYKIWYVVTSSYFEYLMFFLITLNTISLGMQHYGQ-TAEFSYMSDILNVAFTGIFTVEMFLKLA----AFKAKGYFGDPWNVFDFLIVIGSVIDVILSEIDTPGIPATPGAEESSRISITFFRLFRVLRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIAL-VDGTHIN---------------------------RNSNFQTFPQAVLLLFRCATGEAWQEIL---LACSYGKLCDPMSDFQPGEEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNF 1371 HSP 3 Score: 136.732 bits (343), Expect = 2.414e-31 Identity = 153/672 (22.77%), Postives = 292/672 (43.45%), Query Frame = 0
Query: 252 RFFIRSTVKQQWFYWFVIILVFFNTVCVAV---EHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAG--------SFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN----FQDG----------------------------------TPPSN----FNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYD------DDIFVQKPILPYSSMFILSS--SNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQE 858
R F + V+ + F +++ +F N V +A+ D L E++FL +F E +++ A G Y + +N D ++ VF V+ ++ G F + LRA R+LR ++ SL+ ++ S++ +M ++ + L+ I+I+A++G++LF G + F+D P+N F+ F A+LTV+Q +T E W EV+Y ++ G +YF+ L L G++ +LN+ L + +E A+++ + E A ++ L ++ + + +ME R+G S + D ++ + I + + F +R +V FF +++II+++++ +A E Q S + V +F +EM++KI LGF SY ++N D+ VV L+ S L I LR +R+LR K + L + ++NS++++ ++L++ LF IFA++ +Q+F GKF F E+ + ++ FD A++T+F + T E W A++ N + A GP + + I++I+ F+ + +N+F+A+ + E
Sbjct: 41 RKFCINIVEWKPFEMIILLTIFANCVALAIFLPMPEDDTNSTNSVLEKVEYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQCSINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWP----WIYFVSLILLGSFFVLNLVLGVLSGEFTKERE--KAKSRGAFQMLREQQAMDEDLRGYLDWITHAEVMDP-DMEPRDGFSQLEEGGSETDSLYEIEGINKFIAFFRQWRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDS-LTKAQDIANEVLLALFTMEMIVKIYALGF----QSYFMSLFNRFDSFVVCTGLLEVMLVASDIMSPLG-ISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDF---------EDL---------------------EVRRSTFDTFPQALITVFQILTGEDWTAVMYNGIMAY---GGPT-YSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAE 665 HSP 4 Score: 126.716 bits (317), Expect = 3.479e-28 Identity = 112/438 (25.57%), Postives = 194/438 (44.29%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQ--SERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLST--------IKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGK-----FFFCTD--------KSKPNAEECQGEYFVFSSYNRPPMVFPRLW----QKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDG---------EIDKNQKACIDFAIQARPLERYMPKERDSFK------------------------YKIWR------------VVVSTPFEYFIMILIVLNTILL 925
++F L NP R F IV + F+ I++ I + +ALA P+ E + N VL +Y+F IF +E LKI+ GFILH +Y R+ WNILD +V+ + + + S Q L +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A++ ++LF+GK +F TD K P + QG S N R W HFDN AMLT++ T EGW +L +++ + I+++ ++ FF +N+ + ++ F ++ E G +D++ + +D+ A ++ M + RD F ++ WR +V S F + ++I+I+LNT+++
Sbjct: 30 ALFCLGLQNPFRKFCINIVEWKPFEMIILLTIFANCVALAIFLPMPEDDTNSTNSVLEKVEYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQ---CSINGSEC---RGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWP------------WIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQMLREQQAMDEDLRGYLDWITHAEVMDPDM-EPRDGFSQLEEGGSETDSLYEIEGINKFIAFFRQWRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVII 448
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|6165983|sp|O73700.1|CAC1D_CHICK (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=CHCACHA1D; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0 Identity = 438/943 (46.45%), Postives = 597/943 (63.31%), Query Frame = 0
Query: 51 CNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEE--RSHIMEARKRAANKRKKMQKLGKSR-------STETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFF-------------IRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDG-TPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGG-HRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNME-KQLQFMHGTQK-----------LSELEMELRNGRSAYD-------------------------DDIFVQKPILPY---SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
C N + C W GPN GIT+FDN FAMLTVFQC+TMEGWT +LYW NDA+G + IYFV+LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLR +QQLE +L GY+DWI +AE++ + DEE R+ + A K+ ++ G+S ++ET+ + E G + A CC + + +F R+ VK FYW VI+LVF NT+ ++ EHY+QP WLT A V L LF EM+V+MY+LG + YF S FNRFDC V+ + E + +E+ G+SV R +RLLRIFKVT++W+SL NLV SLL+SM+SI SLL LLFLFI+IF+LLGMQLFGG FNF + T S F+ F ALLTVFQ+LTGEDWN VMY GI + GG SGMI C+YFIIL + GNY LLNVFLAIAVDNLA+A+ L A+ + E + + A ++E K+ + G QK E E E ++ D ++ +++ I P S+ FI SS+NPIR+ H ++ F I+V I++SS++LAAEDP++ S RN++L Y DYVFT +F E++LK+ G LH GS+CR+ +N+LD +VV +LV+FG Q S+ +S +K LRVLRVLRPL+ I R LK V CV +++ + NI+IV L QF+FA I VQLF GKF+ CTD++K N EEC+G Y V+ + PMV R+WQ F+FDNV +AM+ LF V T EGWPA+L ++ + E+ GPV +R+E+SIF+I++ I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+++A++ARPL RY+PK + ++YK W VV ST FEY + +LI+LNT+ L M+
Sbjct: 321 CVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPA----------CCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVE-LEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPK--NPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQ 1246 HSP 2 Score: 132.494 bits (332), Expect = 5.793e-30 Identity = 107/382 (28.01%), Postives = 175/382 (45.81%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--------GSSTGQNLS--TIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGK-----FFFCTD---KSKP--------------NAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGE---------IDKNQKACIDFAIQARPLERYMPKERD 895
P ++F LS +NPIR +V + FD FI++ I + +ALA P E NH L +Y F IF VE LKII G +LHP +Y R+ WN+LD V+V L + + GS +G +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A+I ++LF GK F +D + P N EC+G + V P +FDN A AMLT+F T EGW +L +++ + I+++ I+ FF +N+ + ++ F ++ E G+ ++++ K +D+ QA ++ +E D
Sbjct: 97 PPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGW-----------VGP---NGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWP------------WIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEAD 452 HSP 3 Score: 122.865 bits (307), Expect = 5.671e-27 Identity = 180/890 (20.22%), Postives = 356/890 (40.00%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNS-----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEP---------------DEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRST-----------VKQQWFYWFVIILVFFNTVCVAVEHYDQPQ-WLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN-------------------FQDGTPPS-------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLG---FILHPG---SYCRDVWNILDAVVVTCALVAFGF---------------QGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWP----AILQNSMAATYEDRGPVPLFRIEMS---IFYIVFFIVFPFFFVNIFVALIIITFQ 857
++FDN A+LTVFQ +T E W +++Y A G +S IYF+ L + G++ +LN+ L + A + E + Q ++++ A + + + ++S E + K+ K K+ + E +D+D +P ++ + R R ++ ++ + FFI S+ + F +++ + ++V +A E + + + L YA++VF +F E++++M A G ++ S FN D +V+ S+ ++ + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G F ++DG + NF+ A++ +F V T E W ++Y+ I+S G G +Y ++FII + + ++N+F+ + Q + E + + +N + +++ + L ++ K Y ++++S+ F+ + V+I+++++ LA + Q + N + + VFTG+F VEMVLK+I F+ Y D WN D+++V ++V G+S +I R+ RV+R +K + R ++ + + S + + + ++ + FI+AVI +Q+F GK + Q FP+ A+L LF T E W A L D P + + I++I F+++ F +N+FVA+I+ F
Sbjct: 707 STFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLA-DAESLNTAQKEEAEEKERKKN------------------ARKESLENKKSEKSEGDQ----KKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSF----GIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNG-ENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTF-----------QEQGEQEYKNCELDKNQRQCVEYALKARPLRR----------------YIPKNPYQYKFWYVVNSTG--------------FEYIMFVLIMLNTLCLAMQHYGQSKL-FNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVF-GKVAMRDNNQINRNNNFQ--------------TFPQ--------------AVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFD 1497 HSP 4 Score: 85.5001 bits (210), Expect = 1.490e-15 Identity = 75/308 (24.35%), Postives = 135/308 (43.83%), Query Frame = 0
Query: 257 STVKQQWFYWFVIILVFFNTVCVAVEHY-----DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVE----------MAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF------------QDGTP-----------------------P----SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYC----LYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAA 502
S V+ + F F+++ +F N CVA+ Y D L E+ FL +F E +++ A G P Y + +N D +++V +F V+ + G F + LRA R+LR ++ SL+ ++ S++ +M ++ + L+ I+I+A++G++LF G + +D P P +NF+ FA A+LTVFQ +T E W +V+Y ++ G C +YF+ L + G++ +LN+ L + + +E A
Sbjct: 116 SLVEWKPFDIFILLSIFAN--CVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIG--------CEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKA 413
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|5921703|sp|Q25452.1|CAC1M_MUSDO (RecName: Full=Muscle calcium channel subunit alpha-1; AltName: Full=MDL-alpha-1) HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0 Identity = 423/946 (44.71%), Postives = 586/946 (61.95%), Query Frame = 0
Query: 25 ETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--QDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRS-GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAE--------------NQAENED----------------------QNETTAASRNMEKQLQFMHGT-QKLSELEMELRNGRSAYDDDIFVQKPILPY-SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
+T N + D A + C S C + W GPN GIT+FDN G +MLTVFQC+T+EGWT +LY DAMG+S+ IYFV+++++G+FF++NL+LGVLSGEF+KER + +NR F KLR +QQ+E +L GY+DWI +AE++ D + I + K NK K++ D+ D +E S FR++ + R +R+R R VK Q FYW +I+LVF NT +A EHY QP WL F Y F+ LF EM+++MY+LG + YF S FNRFDC V++ S+ E++ T+ E+ G+SVLR +RLLR+FKVTKYW SL NLV SLL+S++SI SLL LLFLFI+IF LLGMQ+FGG F F ++ P SNF++F +LLTVFQ+LTGEDWN VMY GI + GG S G++ C+Y+IIL + GNY LLNVFLAIAVDNLA+A L+ E N+ ENE+ Q+E + + E + G Q++S L R + D+ K +PY +S FI S +N RIF H + F FI+ I+ SS LAAE+P++ + RN VL FDY FT +F +E+VLK+I GF+LH G++CR +N+LD +VV +L++ F ++ +S +K LRVLRVLRPL+ I R LK V CV+ ++K + NI++V L QF+FAVI VQLF GKFF C+D SK +C G Y + + + P + R W+ KFHFD+VA AMLTLF V T EGWP +L S+ + E+ GP+ FR ++ +YI++ I+ FF VNIFV +I+TFQ +GE E ++ E+DKNQ+ CI+FA++A+P+ RY+PK S +YK+W V S+ FEY I +LI++NT+ L MK
Sbjct: 222 KTCRHSNTGEYLNDLDELHACGVGFKCPSGYE-CFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWEWIYFVSMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDI------EPDPDAQIIEDCHK---NKVKEV--------VSIDNLKDHENETQQTDSWFRSQKK-------YLERINRRIRRACRKAVKSQAFYWLIILLVFLNTGVLATEHYRQPIWLDQFQEYTNIFFIALFTCEMILKMYSLGFQGYFVSLFNRFDCFVVIGSISEMVLTSS-ELMAPLGVSVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEEKPRSNFDSFYQSLLTVFQILTGEDWNVVMYDGIRAYGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQIQLDNQIKNEMENEEYLQNGDHISFKAEFGADLDTYLQDEECGSYSDDENTYNKLGGVKQRVSSLP------RRNTNTDMDRIKKDIPYGTSFFIFSHTNRFRIFCHRLCNHSNFGNFILCCIMFSSAMLAAENPLKADASRNIVLNKFDYFFTAVFTIELVLKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISIFFNSNA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFSCSDGSKVYESDCHGTYLFYENGDINKPRLKEREWKNNKFHFDDVAKAMLTLFTVSTFEGWPTLLYVSIDSNKENGGPIYNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKH--SIQYKVWWFVTSSSFEYSIFVLIMINTVTLAMK 1129 HSP 2 Score: 143.28 bits (360), Expect = 2.354e-33 Identity = 163/741 (22.00%), Postives = 316/741 (42.65%), Query Frame = 0
Query: 211 TETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRV---------RFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTD----FLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN---------------------------------FQDGTPP----SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNE---------------TTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALII---------ITFQEQGEAELQDGEIDKNQ 873
TETD+ ++ Q + R + +GG QQ R E+ + R F V + F +F+++ +F N V +AV + P ++ L E++FL +F +E V+++ A G Y + +N D ++V + +N V+ F + LRA R+LR ++ SL+ ++ S+L +M ++ + L+ I+I+A++G++LF G + F D P +NF+ F +++LTVFQ +T E W +V+Y ++ G +YF+ + + G + ++N+ L + ++E T A+N+ + + E T A + Q + K E+ + ++++ ++ + IR V F I++++ +++ LA E ++ + Y + F +F EM+LK+ LGF Y ++N D VV ++ S L + LR +R+LR K K L + ++NS++++ ++L++ LF IF ++ +Q+F G+F F ++ KP + +FD+ ++LT+F + T E W ++ + + A G V F I I+YI+ FI + +N+F+A+ + T +++ E+++Q KN+
Sbjct: 8 TETDNVQNK------QKATTPKRTQRRGGK-QQLDRPERALFCLTLKNPLRIFCIKIVDSKLFEYFILLTIFANCVALAV-YTPYPSGDSNITNQMLEKIEYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTALSNLVKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWEW----IYFVSMVILGAFFVMNLILGVLSGEF--SKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDPDAQIIEDCHKNKVKEVVSIDNLKDHENETQQTDSWFRSQKKYLERINRRIRRACRKAVKSQAFYWLIILLVFLNTGVLATEH-YRQPIWLDQFQEYTNIFFIALFTCEMILKMYSLGF----QGYFVSLFNRFDCFVVIGSISEMVLTSSELMAPLG-VSVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEEKPRS-----------------------------NFDSFYQSLLTVFQILTGEDWNVVMYDGIRAY----GGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQIQLDNQIKNE 693 HSP 3 Score: 138.658 bits (348), Expect = 5.804e-32 Identity = 98/356 (27.53%), Postives = 171/356 (48.03%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF----------FCTDKSKPNAE----ECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGE---------IDKNQKACIDFAIQARPLE 887
P ++F L+ NP+RIF IV F+ FI++ I + +ALA P N +L +Y+F IF E V+KII GF+LH GSY R+ WN LD +V +++ S+ + +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A+I ++LF+GK + D + +A +C Y F + P +FDN +MLT+F T EGW +L Q++M +++E I+++ I+ FF +N+ + ++ F ++ G+ I+++ + +D+ QA +E
Sbjct: 37 PERALFCLTLKNPLRIFCIKIVDSKLFEYFILLTIFANCVALAVYTPYPSGDSNITNQMLEKIEYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTAL--SNLVKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDWVGP--------NDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWE------------WIYFVSMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIE 370 HSP 4 Score: 119.013 bits (297), Expect = 7.327e-26 Identity = 176/869 (20.25%), Postives = 351/869 (40.39%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFN-----SIYFVTLIVIGSFFMLNLVLGVLSGEFAKER-----ERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLG--KSRSTE-----TDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDF-LYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMW-TNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-NFQDGTP--------------------PS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSST------------------GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPV-----PLFRIEMSI---FYIVFFIVFPFFFVNIFVALIIITFQ 857
++FD+ ++LTVFQ +T E W ++Y A G F+ IY++ L + G++ +LN+ L + A E+ + Q L + + ++E E +++ + + E D + E ++ KLG K R + T+ D D + + ++F + R R F F F++ + F++ +A E+ + + L ++ F +F E+V+++ + G + S+FN D +V+ S+ + + +N + + + +LR LR+LR + L+++V ++ ++++I +++ + L +FA++G+QLF G F + DG+ P +F+ A A+LT+F V T E W ++Y I+S G IY I+ + Y ++ F + N+ + +N+ E E +N +N ++F + + ++ K + Y + ++SS+ F+ I V+I+++++ LA + Q + + +L + +FT +F++E + K+ F +Y D WN D ++V + + + T G L +I R+ RV+R +K + + ++ + + S + + + ++ ++ FI+AVI +Q+F GK S Q FP+ A+L LF T E W I+ MA + D + SI ++I F+++ F +N+FVA+I+ F
Sbjct: 598 SNFDSFYQSLLTVFQILTGEDWNVVMYDGIRAYGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQIQLDNQIKNEMENE-----EYLQNGDHISFKAEFGADLDTYLQDEECGSYSDDENTYNKLGGVKQRVSSLPRRNTNTDMDRIKKDIPYGTSFF------------IFSHTNRFRIFCHRLCNHSNFGNFILCCIMFSSAMLAAENPLKADASRNIVLNKFDYFFTAVFTIELVLKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISIFFNSNAISV-----VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFSCSDGSKVYESDCHGTYLFYENGDINKPRLKEREWKNNKFHFDDVAKAMLTLFTVSTFEGWPTLLYVSIDSNK-ENGGPIYNFRPIVAAYYIIYIIIIAFFMV---NIFVGFVIVTFQNEGEQEYKN--CELDKNQRNCIEFALKAKPVRR----------------YIPKHSIQYKVWWFVTSSS--------------FEYSIFVLIMINTVTLAMKFYKQPEY-YSEILDALNMIFTAVFSLEFIFKLAAFRF----KNYFGDAWNTFDFIIVLGSFIDIVYSEIKTKEQALATCDGQSCNKAKGGSTLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIMLEEGTSIDRNNNFQ--------------TFPQ--------------AVLVLFRSATGEAWQEIM---MACSPRDDVKCDPESDAVNNCGSSIAFPYFISFYVLCSFLIINLFVAVIMDNFD 1371
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|1209676875|sp|Q02485.2|CAC1S_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=ROB1; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 745.347 bits (1923), Expect = 0.000e+0 Identity = 427/950 (44.95%), Postives = 578/950 (60.84%), Query Frame = 0
Query: 29 EGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDE--PDEGLSQASAFRTRLRNQGGCCQQFWRCEKRV-RFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDG-TPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENE-------------DQNETTAASRNMEKQLQF----MHGTQKLSELEMELRNGRSAYDDDIFVQK---------------------------PILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPM-VFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
E K PC + C N S C W GPN+GIT FDN GF+MLTV+QC++MEGWT +LYW NDA+GN + IYFVTLI++GSFF+LNLVLGVLSGEF KERE+ ++R F KLR +QQLE +L GY+ WI + E + + + R GK E D + EGL++ F +R+ WR RV R+ VK + FYW VI++V NT+ +A EH++QP WLT A V L LF EM+++MY LG R YF S FNRFDC V+ + + E++ M G+SVLR +RLLR+FK+TKYW+SL NLV SLL+S+RSI SLL LLFLF++IFALLGMQLFGG ++F+D SNF+ F AL++VFQVLTGEDWN VMY GI + GG G++ C+YFIIL + GNY LLNVFLAIAVDNLA A+ LT+A+ E + E + ++ +E++ + K+ E E + + Y F PI SS FI S +N IR+ H IV +F FI++ I++SS ALAAEDP++ S RN +L YFDYVFT +F VE+VLK+ G LH GS+CR+ +NILD +VV +L++ G + S+ +S +K LRVLRVLRPL+ I R LK V CV +++ + NI++V L QF+FA I VQLF GKF+ C D SK EEC+G Y+++ + + + PR W FHFDNV +AM++LF V T EGWP +L ++ + ED GPV R+EM+IF+I++ I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+ +A++ARPL Y+PK + ++Y++W VV S+ FEY + LI+LNTI L M+
Sbjct: 238 ENEKPSPCARTGSGRP------CTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLRE-------------------------GKLSLDEGGSDTESLYEIEGLNKIIQF---IRH--------WRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMT-PLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPK--NPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQ 1138 HSP 2 Score: 147.902 bits (372), Expect = 9.726e-35 Identity = 187/887 (21.08%), Postives = 363/887 (40.92%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAM---GNSFNS----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLEREL-----------NGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQ-------FWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTD-FLYYAEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN-------------------FQDGTPPS-------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER-NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSST--------------GQNLSTIKSLRV-------LRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATY-------EDRGPVPLFRIEMSI---FYIVFFIVFPFFFVNIFVALIIITF 856
++FDN A+++VFQ +T E W S++Y N M G S+ IYF+ L V G++ +LN+ L + A+ ++A + R+++++ R L ++ K E + + DE S++ E + S DD++DEP+ S L+ + + ++R V WF F+++ + ++ +A E + + + L Y ++VF +F E+V++M G ++ S FN D +V+ S+ + +E + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G F ++DG P +F+ A++++F V T E W +++Y+ I+S +G +Y ++FII + + ++N+F+ + + Q E E +N +N + +Q+ + L ++ K NP + + +VT +F+ + +I++++I L + QSE+ NH+ + FT IF +EM+LK+I P Y D WN+ D ++V +++ T N+ +S R+ RV+R +K + R ++ + + S + + + ++ ++ FI+AVI +Q+F GK D ++ N + +F A+L LF T E W IL +A +Y D P + + ++I F+++ F +N+FVA+I+ F
Sbjct: 595 SNFDNFPQALISVFQVLTGEDWNSVMY--NGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYP-----------SADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISM----GLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSN-EEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVI---------VTFQEQGETEYKN--CELDKNQRQCVQYALKARPLR----------------CYIPK--------------NPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQH--YNQSEQMNHISDILNVAFTIIFTLEMILKLI----AFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMF-GKIAM-VDGTQIN---------------------------RNNNFQTFPQAVLLLFRCATGEAWQEIL---LACSYGKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNF 1384 HSP 3 Score: 126.331 bits (316), Expect = 3.653e-28 Identity = 148/671 (22.06%), Postives = 289/671 (43.07%), Query Frame = 0
Query: 257 STVKQQWFYWFVIILVFFNTVCVAV---EHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVF-------EVMWTNCVEMAG-SFGLSV--LRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN-------------------------------------FQDGTPPSN-----FNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQ-FMHGTQKLSELEM-ELRNGRSAYDDDIFVQKPILPYSSM-----FILSSSNPIRIF---IHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQE 858
S V+ + F +++ +F N V +AV D L L E+ FL +F E +++ A G Y S +N D I++ VF ++ TN M+ GL V LRA R+LR ++ SL+ ++ S+ +M + + L+ ++I+A++G++LF G + + G P N F+ F ++LTV+Q ++ E W +V+Y ++ G +YF+ L L G++ +LN+ L + + E T +A++ + + +E+ L+ +M + +++ +LR G+ + D+ + + + FI R+F H +V F +++I+ ++++++A+E Q H+ + V +F +EM++K+ LG Y ++N D VV ++ S L I LR +R+LR K K L + ++NS++++ ++L++ LF IFA++ +QLF G++ F + + + +FDN A++++F V T E W +++ N + A P L + I++I+ F+ + +N+F+A+ + E
Sbjct: 46 SVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWP----WIYFVTLILLGSFFILNLVLGVL------SGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLW-LTHLQDVANRVLLALFTIEMLMKMYGLGL----RQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLG-ISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVR------------------------------RSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVL----VCIYFIILFVCGNYILLNVFLAIAVDNLAE 666 HSP 4 Score: 117.857 bits (294), Expect = 1.667e-25 Identity = 91/342 (26.61%), Postives = 155/342 (45.32%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQG-----------SSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGK-----FFFCTD--------KSKPNAEECQGEYFVFS-SYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDK 871
P ++F L+ NP+R +V + F+T I++ I + +ALA P+ E N L +Y F +F++E +KII GF+ H +Y R WN+LD ++V + + SS G L +K+LR RVLRPL+ + VP L+ V + + ++ +F+I ++ L I+A+I ++LF GK +F TD K P A G + S R P HFDN +MLT++ + EGW +L +++ + I+++ ++ FF +N+ + ++ F ++ E G K
Sbjct: 27 PPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLD-VKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGP---NHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWP------------WIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQK 352
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|308153651|sp|Q13936.4|CAC1C_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 741.11 bits (1912), Expect = 0.000e+0 Identity = 436/979 (44.54%), Postives = 595/979 (60.78%), Query Frame = 0
Query: 29 EGNKNVPCHSD-DPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILA-EERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKR-VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQD-GTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAE----------------NQAENEDQNETTAASRNMEKQLQFMHGTQ----------KLSELEMELRNGRSAYDD------------------------DIFVQKPILPY---SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYF--------------------DYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
EG +VP D P G N ++C W+GP +GIT+FDN FAMLTVFQC+TMEGWT +LYW NDA+G + IYFVTLI+IGSFF+LNLVLGVLSGEF+KERE+ + R F KLR +QQLE +L GY+DWI +AE++ E+ DEE+ R ++ S T++ EG + + R+ ++WR R R R+ VK FYW VI LVF NT+ +A EHY+QP WLT+ A L LF EM+++MY+LG + YF S FNRFDC V+ + E + ++ G+SVLR +RLLRIFK+T+YW+SL NLV SLL+S+RSI SLL LLFLFI+IF+LLGMQLFGG FNF + T S F+ F +LLTVFQ+LTGEDWN VMY GI + GG GM+ C+YFIIL + GNY LLNVFLAIAVDNLA+A+ LT+A+ + + ++ E A + E++++ T + +L+ +S Y + ++ +++ +P S+ FI SS+N R+ H IV F I+ I++SSI+LAAEDPVQ S RNH+L YF DYVFT IF +E++LK+ G LH GS+CR+ +NILD +VV+ +L++FG Q S+ ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI+IV L QF+FA I VQLF GK + C+D SK EC+G Y + P++ PR W+ KF FDNV AAM+ LF V T EGWP +L S+ + ED+GP+ +R+E+SIF+I++ I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+++A++ARPL RY+PK + +YK+W VV ST FEY + +LI+LNTI L M+
Sbjct: 302 EGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKP------------RNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK--FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVE-TKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSA----INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPK--NQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQ 1259 HSP 2 Score: 123.635 bits (309), Expect = 3.356e-27 Identity = 96/374 (25.67%), Postives = 166/374 (44.39%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLST---------IKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFF------------CTDKSKP------NAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGE---------IDKNQKACIDFAIQARPLERYMPKERD 895
P ++ L+ NPIR IV + F+ I++ I + +ALA P E N L +Y+F IF VE LK+I G + HP +Y R+ WN+LD ++V L + + ++ + +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A+I ++LF GK D P + +CQ ++ P + +FDN A AMLT+F T EGW +L A D P I+++ I+ FF +N+ + ++ F ++ E G+ ++++ K +D+ QA ++ P+ D
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGP--------KHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRD---WPW------IYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDID---PENED 453 HSP 3 Score: 113.235 bits (282), Expect = 5.302e-24 Identity = 150/681 (22.03%), Postives = 282/681 (41.41%), Query Frame = 0
Query: 262 QWFYWFVIILVFFNTVCVAVEHY-----DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS---------FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN------------------------------FQDGT--PP---------SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQE---------------------------LTAAEN-QAENEDQNETTAASRNMEKQLQFMHG--TQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALII 853
+W + +IIL+ CVA+ Y D L E++FL +F E +++ A G P Y + +N D I++V +F + + G+ F + LRA R+LR ++ SL+ ++ S++ +M ++ + L+ I+I+A++G++LF G + Q+GT P +NF+ FA A+LTVFQ +T E W +V+Y ++ G +YF+ L + G++ +LN+ L + + +E +T AE+ ENED+ RNM T+ ++ ++E N + I K S + + R V F ++ ++ ++++ +A+E + + V + +F EM+LK+ LG +Y ++N D VV ++ + L I LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF F D+ Q ++ FDN ++LT+F + T E W +++ + + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 122 EWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWP----WIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK-----FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFVVCGGILETILVETKIMSPLG-ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF--DE----------------------------MQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 753 HSP 4 Score: 109.383 bits (272), Expect = 8.012e-23 Identity = 184/932 (19.74%), Postives = 366/932 (39.27%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGN-SFNS----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSR-STETDDDDDEPDEGLSQAS-------------------AFRTRLRNQGGCCQQFWRCEKRVRFFIRST-----------VKQQWFYWFVIILVFFNTVCVAVEHYDQ-PQWLTDFLYY--------------------AEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDG-------TPPS------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDL---GFILHP----------GS-----------YCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSL-------------RVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAA---------TYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITF 856
++FDN ++LTVFQ +T E W S++Y A G SF IYF+ L + G++ +LN+ L + A ++ + + +++L R + K +E++ E+ E + +E + A+ G+S +T+ + DD +P+E ++ R R ++ ++ + FFI S+ V F ++ + +++ +A E Q + L+Y A++VF +F E++++M A G ++ S FN D +V+ S+ ++ + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G ++DG P S +F+ A++ +F V T E W E++Y+ I+S + G IY ++FII + + ++N+F+ + Q + E + + +N + +++ + L ++ K Y ++++S+ +F+ + V+I++++I LA + + + + +FTG+F VEM+LK+I G+ P GS Y D WN DA++V ++V + ++ S+ R+ RV+R +K + R ++ + + S + + + ++ ++ FI+AVI +Q+F GK D ++ N + +F A+L LF T E W I+ M + G P ++I F+++ F +N+FVA+I+ F
Sbjct: 687 STFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPE-----KKQELV--EKPAVGESKEEKIELKSITAD--------GESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISF----GIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDK-GPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTF-----------QEQGEQEYKNCELDKNQRQCVEYALKARPLRR----------------YIPKNQHQYKVWYVVNST--------------YFEYLMFVLILLNTICLAMQH-YGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIAL-NDTTEIN---------------------------RNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNF 1527
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|116411|sp|P15381.1|CAC1C_RABIT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Smooth muscle calcium channel blocker receptor; Short=CACB-receptor; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 740.725 bits (1911), Expect = 0.000e+0 Identity = 433/959 (45.15%), Postives = 594/959 (61.94%), Query Frame = 0
Query: 29 EGNKNVPCHSD-DPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILA-EERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKR-VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQD-GTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMH--GTQKLSELEMELRN------------------------GRSAYDD------------------------DIFVQKPILPY---SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
EG +VP D P G N ++C W+GP +GIT+FDN FAMLTVFQC+TMEGWT +LYW DAMG +YFV+L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLR +QQLE +L GY+DWI +AE++ E+ DEE+ R ++ S T++ EG + + R+ ++WR R R R+ VK FYW VI LVF NT+ +A EHY+QP WLT+ A L LF EM+++MY+LG + YF S FNRFDC ++ + E + M+ G+SVLR +RLLRIFK+T+YW+SL NLV SLL+S+RSI SLL LLFLFI+IF+LLGMQLFGG FNF + T S F+ F +LLTVFQ+LTGEDWN VMY GI + GG GM+ C+YFIIL + GNY LLNVFLAIAVDNLA+A+ LT+A+ + E E + + A + + EK+ + + ++ E ++EL++ +S Y + ++ +++ +P S+ FI S +N R+ H IV F I+ I++SSI+LAAEDPVQ S RNH+L YFD VFT IF +E+ LK+ G LH GS+CR+ +NILD +VV+ +L++FG Q S+ ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI+IV L QF+FA I VQLF GK + C+D SK EC+G Y + P++ PR W+ KF FDNV AAM+ LF V T EGWP +L S+ + ED+GP+ +R+E+SIF+I++ I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+++A++ARPL RY+PK + +YK+W VV ST FEY + +LI+LNTI L M+
Sbjct: 332 EGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEK------------PRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK--FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKVMS-PLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMDDLQPNESEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSA----INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPK--NQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQ 1269 HSP 2 Score: 124.02 bits (310), Expect = 2.138e-27 Identity = 96/343 (27.99%), Postives = 154/343 (44.90%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLST---------IKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFF------------CTDKSKPNA-EECQGEYFVFSSYNRPPMVFPRLWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFV-NIFVALIIITFQEQGEAELQDGEIDK 871
P ++ L+ NPIR IV + F+ I++ I + +ALA P E N L +Y+F IF VE LK+I G + HP +Y R+ WN+LD ++V L + + ++ + +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A+I ++LF GK D P A E G + +P W K +FDN A AMLT+F T EGW +L Q++M E+ Y V ++F FFV N+ + ++ F ++ E G+ K
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPG------WDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 453 HSP 3 Score: 120.939 bits (302), Expect = 1.966e-26 Identity = 175/881 (19.86%), Postives = 347/881 (39.39%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGN-SFNS----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVI----LAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDE---------------GLSQASAFRTRLRNQGGCCQQ-----FWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQ-PQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDG-------TPPS------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSL-------------RVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATY---------EDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITF 856
++FDN ++LTVFQ +T E W S++Y A G SF IYF+ L + G++ +LN+ L + A ++ + + +++L R + K +EV+ L E + E I + + M L + E++D P+ G L+ + + + R R V F ++ + +++ +A E Q + L+Y + VF +F E+ ++M A G ++ S FN D +V+ S+ ++ + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G ++DG P S +F+ A++ +F V T E W E++Y+ I+S + G IY ++FII + + ++N+F+ + Q + E + + +N + +++ + L ++ K Y ++++S+ +F+ + V+I++++I LA + + + + +FTG+F VEM+LK+I P Y D WN+ D ++V +++ ++ ++ S+ R+ RV+R +K + R ++ + + S + + + ++ ++ FI+AVI +Q+F GK D ++ N + +F A+L LF T E W I+ M G P ++I F+++ F +N+FVA+I+ F
Sbjct: 717 STFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPE-----KKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMDDL---QPNESEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISF----GIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDK-GPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTF-----------QEQGEQEYKNCELDKNQRQCVEYALKARPLRR----------------YIPKNQHQYKVWYVVNST--------------YFEYLMFVLILLNTICLAMQH-YGQSCLFKIAMNILNMLFTGLFTVEMILKLI----AFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIAL-NDTTEIN---------------------------RNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPHNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNF 1509 HSP 4 Score: 116.316 bits (290), Expect = 5.192e-25 Identity = 152/685 (22.19%), Postives = 285/685 (41.61%), Query Frame = 0
Query: 262 QWFYWFVIILVFFNTVCVAVEHY-----DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS---------FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN------------------------------FQDGT--PP---------SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQE---------------------------LTAAEN-QAENEDQNETTAASRNMEKQLQFMHG--TQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYF----DYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALII 853
+W + +IIL+ CVA+ Y D L E++FL +F E +++ A G P Y + +N D I++V +F + + G+ F + LRA R+LR ++ SL+ ++ S++ +M ++ + L+ I+I+A++G++LF G + Q+GT P +NF+ FA A+LTVFQ +T E W +V+Y ++ G + +YF+ L +FG++ +LN+ L + + +E +T AE+ ENED+ RNM T+ ++ ++E N + I K S + + R V F ++ ++ ++++ +A+ E + H LT + +F EM+LK+ LG +Y ++N D +V ++ + L I LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF F D+ Q ++ FDN ++LT+F + T E W +++ + + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 152 EWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYE----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK-----FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIAS-----EHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKVMSPLG-ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF--DE----------------------------MQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 783
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|226693506|sp|O60840.2|CAC1F_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1F; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.4) HSP 1 Score: 740.339 bits (1910), Expect = 0.000e+0 Identity = 429/954 (44.97%), Postives = 581/954 (60.90%), Query Frame = 0
Query: 35 PCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRS------------------------------TETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAG--SFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF-QDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGH-RSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELR-----------------------NGRSAYDDDIFVQK--PILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
PC S +A C N + C +W GPN GIT+FDN FAMLTVFQCVTMEGWT +LYW DAMG +YFV+L++ GSFF+LNLVLGVLSGEF+KERE+ + R F K R +QQ+E +L GY+DWI +AEE+ + + D S E R A R ++ +L R TET D+DE + L+ + ++ + C++ R + +R R VK YW V++LVF NT+ +A EH+ QP WLT YA V L LF EM++++Y LGP Y S FNRFDC V+ + E T VE+ G+SVLR +RLLRIFKVT++W+SL NLV SLL+SM+SI SLL LLFLFI+IF+LLGMQLFGG FNF Q T S F+TF ALLTVFQ+LTGEDWN VMY GI + GG GM+ C+YFIIL + GNY LLNVFLAIAVDNLA+ TA + E ++ + + + + G +K E G + + +K PI S+ F LS +NP+R H ++ F I+V II+SS++LAAEDP++ S RNH+L YFDY FT IF VE++LK+ G LH GS+CR +N+LD +VV+ +L++FG S+ +S +K LRVLRVLRPL+ I R LK V CV +++ + NI+IV L QF+FA I VQLF GKF+ CTD++K +EC+G + V+ + P+V RLW F+FDNV +AM+ LF V T EGWPA+L ++ A ED GP+ +R+E+S+F+IV+ I+ FF +NIFV +IITF+ QGE E Q+ E+DKNQ+ C+++A++A+PL RY+PK + +Y++W V S FEY + +LI+LNT+ L M+
Sbjct: 282 PCASSGSGRA------CTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNLGSMAEEGR---AGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNKIM-KTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILE---TTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKDLPQENEGL------VPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSA----ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPK--NPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQ 1210 HSP 2 Score: 130.568 bits (327), Expect = 2.138e-29 Identity = 107/371 (28.84%), Postives = 168/371 (45.28%), Query Frame = 0
Query: 563 SMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAF------GFQGSS--TGQNLS--TIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGK-----FFFCTD-----KSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFV-NIFVALIIITFQEQGEAELQDGEIDKNQ---------KACIDFAIQARPLERYMPKERDSF 897
++F L+ +NP+R IV + FD I++ I + +AL P E NH L +YVF IF VE VLKI+ G +LHP +Y R+ WN+LD ++V L + G G + TG +K+LR RVLRPL+ + VP L V + ++ +L + +I ++ L I+A+I ++LF G+ +F +D P A G + R P +FDN AMLT+F T EGW +L Q++M E+ Y V ++F FFV N+ + ++ F ++ E G+ K + + +D+ QA L+ P D+
Sbjct: 71 ALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGP---NGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPSADDNL 425 HSP 3 Score: 118.242 bits (295), Expect = 1.310e-25 Identity = 80/291 (27.49%), Postives = 144/291 (49.48%), Query Frame = 0
Query: 235 RNQGGCCQQFWRCEKRVRFFIR--------STVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFG----------LSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTP---PSNFNTFAIALLTVFQVLTGEDWNEVMYQGI-------ESQGGHRS----GMIYCL-YFIILTLFGNYTLLNVFLAIAVDN 492
+NQ C + + + R+ + +TV F + + +L+ NTV +A++HY+Q + VF GLF EMV+++ A P+ YF ++N FD +++V S+ ++ T V G G ++ R R++R+ K+ +R L+ + + S +++ + L+ + I+A++GMQ+F G QDGT +NF TF A+L +F+ TGE W E+M + ES G G + + YFI + + ++N+F+A+ +DN
Sbjct: 1155 KNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTE-VNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMF-GKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDN 1443 HSP 4 Score: 116.316 bits (290), Expect = 5.085e-25 Identity = 135/665 (20.30%), Postives = 268/665 (40.30%), Query Frame = 0
Query: 251 VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQ-WLTDFLYYAEFVFLGLFITEMVVRMYALGPRIY----FESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN-------------------FQDGTPPS-------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFII------LTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLS---------TIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATY----EDRGPVPLFRIEMSI---FYIVFFIVFPFFFVNIFVALIIITF 856
+R + + F +++ + ++V +A E + + L Y ++ F +F E++++M G ++ S FN D +V+ S+ + + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G F + DG NF+ A++ +F V T E W ++Y+ I++ G IY I + + ++N+F+ + +A+ E + + +N + +++ Q L ++ K NP + + V F+ + ++I+++++ALA + ++ + N+ + + VFTG+F +EMVLKII P Y D WN DA++V ++V + G +L +I R+ RV+R +K + + ++ + + S + + + ++ + FI+AVI +Q+F GK D ++ N + +F A+L LF T E W I+ S+ D GP F + ++I FF++ F +N+FVA+I+ F
Sbjct: 860 LRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISF----GIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYA-EDHGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITF-----------RAQGEQEYQNCELDKNQRQCVEYALKAQPLRR----------------YIPK--------------NPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQH-YEQTAPFNYAMDILNMVFTGLFTIEMVLKII----AFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMF-GKVAL-QDGTQIN---------------------------RNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNF 1444 HSP 5 Score: 90.8929 bits (224), Expect = 3.283e-17 Identity = 82/338 (24.26%), Postives = 153/338 (45.27%), Query Frame = 0
Query: 262 QWFYWFVIILVFFNTVCVAVEHY-----DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMW----------TNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF------------QDGTP---------------------P------SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQ-FMHGTQKLSELEME 540
+W + ++IL+ CVA+ Y D L E+VFL +F E V+++ A G P Y + +N D I++V +F V+ + G F + LRA R+LR ++ SL ++ S++ ++ ++ + L+ I+I+A++G++LF G + +D +P P +NF+ F A+LTVFQ +T E W +V+Y ++ G + +YF+ L +FG++ +LN+ L + + +E A+ + + + Q E + ME+ L+ ++ + EL+ME
Sbjct: 90 EWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYE----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKERE--KAKARGDFQKQRE----KQQMEEDLRGYLDWITQAEELDME 417
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Match: gi|6165982|sp|Q01815.1|CAC1C_MOUSE (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=MELC-CC; AltName: Full=Mouse brain class C; Short=MBC; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0 Identity = 433/959 (45.15%), Postives = 594/959 (61.94%), Query Frame = 0
Query: 29 EGNKNVPCHSD-DPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILA-EERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKR-VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQD-GTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHR-SGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMH--GTQKLSELEMELR----NGRSA------YDD--------------------------------------DIFVQKPILPY---SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYN-RPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
EG +VP D P G N ++C W+GP +GIT+FDN FAMLTVFQC+TMEGWT +LYW DAMG +YFV+L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLR +QQLE +L GY+DWI +AE++ E+ DE++ R ++ S T++ EG + + R+ ++WR R R R+ VK FYW VI LVF NT+ +A EHY+QP WLT+ A L LF EM+++MY+LG + YF S FNRFDC ++ + E + ++ G+SVLR +RLLRIFK+T+YW+SL NLV SLL+S+RSI SLL LLFLFI+IF+LLGMQLFGG FNF + T S F+ F +LLTVFQ+LTGEDWN VMY GI + GG GM+ C+YFIIL + GNY LLNVFLAIAVDNLA+A+ LT+A+ + E E + + A + + EK+ + M ++ E ++EL+ +G S DD ++ +++ +P S+ FI S +N R+ H IV F I+ I++SSI+LAAEDPVQ S RNH+L DYVFT IF +E++LK+ G LH GS+CR+ +NILD +VV+ +L++FG Q S+ ++ +K LRVLRVLRPL+ I R LK V CV +++ + NI+IV L QF+FA I VQLF GK + C+D SK EC+G Y + P++ PR W+ KF FDNV AAM+ LF V T EGWP +L S+ + ED+GP+ +R+E+SIF+I++ I+ FF +NIFV +I+TFQEQGE E ++ E+DKNQ+ C+++A++ARPL RY+PK + +YK+W VV ST FEY + +LI+LNTI L M+
Sbjct: 302 EGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKP------------RNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK--FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVE-TKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSA----INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPK--NQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQ 1239 HSP 2 Score: 125.946 bits (315), Expect = 5.662e-28 Identity = 176/880 (20.00%), Postives = 356/880 (40.45%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGN-SFNS----IYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAAN-KRKKMQKLGKSRSTETDDDDDEPDEGLSQASAF-----------RTRLRNQGGCCQQFWRCEKRVRFFIRST-----------VKQQWFYWFVIILVFFNTVCVAVEHYDQ-PQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFES----SFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDG-------TPPS------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIY------CLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSL-------------RVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAA---------TYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITF 856
++FDN ++LTVFQ +T E W S++Y A G SF IYF+ L + G++ +LN+ L + A ++ + + +++L R + K +EV+ E+ +E + +E + A+ + K+ ++++D P A R R ++ ++ + FFI S V F ++ + +++ +A E Q + L A++VF +F E++++M A G ++ S FN D +V+ S+ ++ + + +LR LR+LR + L+++V + ++R+I +++ + L +FA +G+QLF G ++DG P S +F+ A++ +F V T E W E++Y+ I+S + G IY ++FII + + ++N+F+ + Q + E + + +N + +++ + L ++ K Y ++++S+ +F+ + V+I++++I LA + + + + +FTG+F VEM+LK+I P Y D WN+ D ++V +++ ++ ++ S+ R+ RV+R +K + R ++ + + S + + + ++ ++ FI+AVI +Q+F GK D ++ N + +F A+L LF T E W I+ M + G P ++I F+++ F +N+FVA+I+ F
Sbjct: 687 STFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPE-----KKQEVM--EKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISF----GIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDK-GPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTF-----------QEQGEQEYKNCELDKNQRQCVEYALKARPLRR----------------YIPKNQHQYKVWYVVNST--------------YFEYLMFVLILLNTICLAMQH-YGQSCLFKIAMNILNMLFTGLFTVEMILKLI----AFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIAL-NDTTEIN---------------------------RNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNF 1479 HSP 3 Score: 124.405 bits (311), Expect = 1.778e-27 Identity = 96/337 (28.49%), Postives = 157/337 (46.59%), Query Frame = 0
Query: 560 PYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSER--NHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLST---------IKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKS---KPNAEECQGEYFVFSSYNRP---PMVFPRLWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFV-NIFVALIIITFQEQGEAELQDGEIDK 871
P ++ L+ NPIR IV + F+ I++ I + +ALA P E N L +Y+F IF VE LK+I G + HP +Y R+ WN+LD ++V L + + ++ + +K+LR RVLRPL+ + VP L+ V + ++ ++ + +I ++ L I+A+I ++LF GK C ++ AEE + + + R V W K +FDN A AMLT+F T EGW +L Q++M E+ Y V ++F FFV N+ + ++ F ++ E G+ K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMG-------------YELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 423 HSP 4 Score: 115.931 bits (289), Expect = 6.907e-25 Identity = 152/685 (22.19%), Postives = 285/685 (41.61%), Query Frame = 0
Query: 262 QWFYWFVIILVFFNTVCVAVEHY-----DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS---------FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN------------------------------FQDGT--PP---------SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQE---------------------------LTAAEN-QAENEDQNETTAASRNMEKQLQFMHG--TQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYF----DYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALII 853
+W + +IIL+ CVA+ Y D L E++FL +F E +++ A G P Y + +N D I++V +F + + G+ F + LRA R+LR ++ SL+ ++ S++ +M ++ + L+ I+I+A++G++LF G + Q+GT P +NF+ FA A+LTVFQ +T E W +V+Y ++ G + +YF+ L +FG++ +LN+ L + + +E +T AE+ ENED+ RNM T+ ++ ++E N + I K S + + R V F ++ ++ ++++ +A+ E + H LT + +F EM+LK+ LG +Y ++N D +V ++ + L I LR +R+LR K + L + ++NS++++ ++L++ LF IF+++ +QLF GKF F D+ Q ++ FDN ++LT+F + T E W +++ + + A P L + I++I+ FI + +N+F+A+ +
Sbjct: 122 EWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYE----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSK-----FSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIAS-----EHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKIMSPLG-ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNF--DE----------------------------MQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGML----VCIYFIILFICGNYILLNVFLAIAV 753
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1022762368|gb|KZS08153.1| (Voltage-dependent calcium channel type A subunit alpha-1 [Daphnia magna]) HSP 1 Score: 1331.62 bits (3445), Expect = 0.000e+0 Identity = 690/929 (74.27%), Postives = 791/929 (85.15%), Query Frame = 0
Query: 24 DETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDE--GLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMH----GTQKL----------------SELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
+E + EG PC++D+ T+A G+Y C+ N S+CLEKWEGPN+GITSFDNIGFAMLTVFQC+TMEGWTSILYWTNDA+G+S+N +YFV LIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYV+WICKAEEVILAEERTT+EE+ HIMEAR+RAA KRKK++ LGKSRST+T+D+++E + G S+AS +++++N+G C++FWR EKR+RF IR TVKQQWFYWFVI+LVFFNTVCVAVEHY+QP WLT+FLYYAEF FLGLF+TEM ++MYALGPRIYFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLR+FKVTKYWSSLRNLVISLLSSMRSI+SLLFLLFLFILIFALLGMQLFGGAFNF +GTPP+NFN+F IALLTVFQ+LTGEDWNEVMYQGIESQGG R GMIY LYFIIL LFGNYTLLNVFLAIAVDNLANAQELTAAE + E + + + +EK+++ +H G+ KL SE + + G + DDDI KP+LPYSSMFILS +NP+R H +V P+FD FIMV+I +SSIALAAEDPV+E S RN +L YFDY FTG+F VEMVLK+ID G ILH GSY R++WN++DAVVV CA V+F F GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVY+LF FIFAV+AVQLFNGKFF+CTD SK ++CQG+YF F+ RPP V R W+ Q FH+DNV AAMLTLFAVQT EGWP +LQNSMAAT ED GP+ +RIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDFAIQA+PLERYMPK+R+SFKYKIWRVVVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 7 NEILAEGELATPCNTDNATQAVAGSYICDYNSSLCLEKWEGPNFGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSSYNWVYFVPLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKLHIMEARRRAAAKRKKLKHLGKSRSTDTEDEENEDEPDEGFSRASYLKSKVKNKG-ACKRFWRAEKRLRFKIRHTVKQQWFYWFVIVLVFFNTVCVAVEHYNQPPWLTEFLYYAEFAFLGLFMTEMCIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWS--AFKSGSFGLSVLRALRLLRVFKVTKYWSSLRNLVISLLSSMRSILSLLFLLFLFILIFALLGMQLFGGAFNFPEGTPPANFNSFPIALLTVFQILTGEDWNEVMYQGIESQGGSRRGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQE---EEDKEKQQQELEKEMEALHMGSDGSPKLDTTSPSKKGKGKRGHKSEGNGDAKTGDNVDDDDIMGPKPMLPYSSMFILSPTNPVRRAAHWVVNLPYFDFFIMVVISLSSIALAAEDPVEEGSFRNDILNYFDYAFTGVFTVEMVLKVIDSGIILHQGSYLREIWNVMDAVVVICAAVSFTFDMMGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYMLFHFIFAVVAVQLFNGKFFYCTDDSKHTQDDCQGDYFTFAYDKRPPEVKRREWKSQLFHYDNVMAAMLTLFAVQTGEGWPQVLQNSMAATQEDHGPILHYRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFAIQAKPLERYMPKDRESFKYKIWRVVVSTPFEYFIMTLIVLNTLLLMMK 929
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|321454423|gb|EFX65595.1| (hypothetical protein DAPPUDRAFT_333049 [Daphnia pulex]) HSP 1 Score: 1331.62 bits (3445), Expect = 0.000e+0 Identity = 692/929 (74.49%), Postives = 791/929 (85.15%), Query Frame = 0
Query: 24 DETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDE--GLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMH----GTQKL----------------SELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
+E + EG PC++D+ T+A G+Y C+ N S+CLEKWEGPN+GITSFDNIGFAMLTVFQC+TMEGWTSILYWTNDA+G+S+N +YFV LIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYV+WICKAEEVILAEERTT+EE+ HIMEAR+RAA KRKK++ LGKSRST+T+D+++E + G S+AS +++++N+G C ++FWR EKR+RF IR TVKQQWFYWFVI+LVFFNTVCVAVEHY+QP WLT+FLYYAEF FLGLF+TEM ++MYALGPRIYFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLR+FKVTKYWSSLRNLVISLLSSMRSI+SLLFLLFLFILIFALLGMQLFGGAFNF +GTPP+NFN+F IALLTVFQ+LTGEDWNEVMYQGIESQGG R GMIY LYFIIL LFGNYTLLNVFLAIAVDNLANAQELTAAE + E + + + +EK+++ +H G+ KL SE + + G + DDDI KP+LPYSSMFILS +NP+R H +V P+FD FIMV+I +SSIALAAEDPV+E S RN +L YFDY FTG+F VEMVLK+ID G ILH GSY R++WN++DAVVV CA V+F F GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVY+LF FIFAV+AVQLFNGKFF+CTD SK ++CQGEYF FS RPP V R W+ Q FH+DNV AAMLTLFAVQT EGWP +LQNSMAAT ED GP+ +RIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDFAIQA+PLERYMPK+R+SFKYKIWRVVVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 201 NEILAEGELATPCNTDNATQAVAGSYICDYNSSLCLEKWEGPNFGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSSYNWVYFVPLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKLHIMEARRRAAAKRKKLKHLGKSRSTDTEDEENEDEPDEGFSRASYLKSKVKNKGAC-KRFWRAEKRLRFKIRHTVKQQWFYWFVIVLVFFNTVCVAVEHYNQPPWLTEFLYYAEFAFLGLFMTEMCIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSAF--KSGSFGLSVLRALRLLRVFKVTKYWSSLRNLVISLLSSMRSILSLLFLLFLFILIFALLGMQLFGGAFNFPEGTPPANFNSFPIALLTVFQILTGEDWNEVMYQGIESQGGSRRGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQE---EEDKEKQQQELEKEMEALHLGSEGSPKLDSTSPSKKGKGKRGHKSEGNGDAKTGDNVEDDDIMGPKPMLPYSSMFILSPTNPVRRAAHWVVNLPYFDFFIMVVISLSSIALAAEDPVEEGSFRNDILNYFDYAFTGVFTVEMVLKVIDSGIILHQGSYLREIWNVMDAVVVICAAVSFTFDMMGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYMLFHFIFAVVAVQLFNGKFFYCTDDSKHTQDDCQGEYFTFSYDKRPPEVKRREWKSQLFHYDNVMAAMLTLFAVQTGEGWPQVLQNSMAATQEDHGPILHYRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFAIQAKPLERYMPKDRESFKYKIWRVVVSTPFEYFIMTLIVLNTLLLMMK 1123 HSP 2 Score: 126.716 bits (317), Expect = 1.021e-25 Identity = 90/326 (27.61%), Postives = 158/326 (48.47%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILD-AVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-----------------FCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FIL+ N IR + I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF LH GSY R++WN++D VVVT ++ F ++ LR RVLRPLK + R+P L+ V ++ ++ + I ++ L IFA+I ++ ++G T + NA + ++ YN + W+ F FDN+ AMLT+F T EGW +IL +++ ++Y ++++ ++ FF +N+ + ++ F ++ E
Sbjct: 11 TSLFILTEKNIIRRYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTPLARKLEKTEPYFLGIFCVESTLKILALGFALHRGSYLRNIWNMMDFVVVVTGSMTIFAEANVEV-----DLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSIEDLNEILAEGELATPCNTDNATQAVAGSYI-CDYNSSLCL--EKWEGPNFGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSSYN------------WVYFVPLIVIGSFFMLNLVLGVLSGEFAKERE 316 HSP 3 Score: 93.5893 bits (231), Expect = 1.419e-15 Identity = 64/249 (25.70%), Postives = 124/249 (49.80%), Query Frame = 0
Query: 264 FYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSF-GLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGT--PPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIES----------QGGH-------RSGMIYCLYFIILTLFGNYTLLNVFLAIAVDN 492
F +F++ L+ NT+ + +++Y Q + L+Y F LF E ++++ + G R +F+ +N FD + +V S+ + + VE +F + LR R R+ K+ + ++R L+ + + S +++ + L+ + I+A++GMQ+FG D + +NF +F LL +F+ TGE W +M I + G S + Y YF+ ++ +LN+F+A+ +DN
Sbjct: 1105 FEYFIMTLIVLNTLLLMMKYYKQSVLYKETLHYMNTAFTALFSIECMLKIISFGVRNFFKDPWNTFDFVTVVGSIIDAL---VVEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNLHLDPDSSVNRHNNFQSFIGGLLLLFRCATGEAWPNIMLSCIRGRQCDPRALKFENGQVIEDKECGSNLAYA-YFVSFIFLCSFLMLNLFVAVIMDN 1349 HSP 4 Score: 72.7886 bits (177), Expect = 2.986e-9 Identity = 73/331 (22.05%), Postives = 142/331 (42.90%), Query Frame = 0
Query: 251 VRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHY---DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRI----YFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGA-----FNFQD----------GTPP---------------------------------SNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQ 526
+R + R ++ F + V++ + N V +A+E + L L E FLG+F E +++ ALG + Y + +N D +V+V + VE+ L +LR+ R+LR K+ SL+ ++ S++ +M ++ + L+ I+IFA++G++ + GA ++ +D TP ++F+ A+LTVFQ +T E W ++Y ++ G + +YF+ L + G++ +LN+ L + A +E + EN + +E++L
Sbjct: 22 IRRYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTPLARKLEKTEPYFLGIFCVESTLKILALGFALHRGSYLRNIWNMMDFVVVVTGSMTIFAEANVEV----DLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSIEDLNEILAEGELATPCNTDNATQAVAGSYICDYNSSLCLEKWEGPNFGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSSYN----WVYFVPLIVIGSFFMLNLVLGVLSGEFAKERE------RVENRQAFLKLRRQQQLERELN 338
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1317988909|ref|XP_023288257.1| (voltage-dependent calcium channel type A subunit alpha-1 [Orussus abietinus]) HSP 1 Score: 1299.26 bits (3361), Expect = 0.000e+0 Identity = 677/927 (73.03%), Postives = 778/927 (83.93%), Query Frame = 0
Query: 24 DETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL------------RNGRSAY------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
++ V EG PC++D+ T+AP+GA+ C+ ++S C+++WEGPN GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G++FN +YF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRR QQLERELNGYV+WICKAEEVILAEERTT+EE+ HI+EAR+RAA KRKK++ LGKS+ST+T++++ E D S+AS +++++N+G C Q FWR EKR RF++R TVK QWFYWFVI+LVFFNT+CVAVEHY QP WLT+FLYYAEFVFLGLF+ EM ++MYALGPRIYFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW+SLRNLVISLL+SMRSIISLLFLLFLFILIFALLGMQLFGG FNF GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFIIL LFGNYTLLNVFLAIAVDNLANAQELTAAE + ED+ + + +EK+++ + S ++E+ + G+ A DD+ KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFFFC+D SK EEC G+YFVF N P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMP +R+S KYKIWR+VVSTPFEYFIMILIVLNT+LLMMK
Sbjct: 212 NQIVKEGEFPSPCNTDNLTEAPLGAHVCDPSVSACMDRWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWVYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRAQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIIEARRRAAAKRKKLKNLGKSKSTDTEEEEGEDDPDDGFSRASYLKSKVKNKGACLQ-FWRAEKRFRFWVRHTVKAQWFYWFVIVLVFFNTICVAVEHYKQPSWLTEFLYYAEFVFLGLFMMEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSAL--KSGSFGLSVLRALRLLRIFKVTKYWASLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFPSGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQM---QELEKEMEALQMPTDGSPPKVEICPPSPTRNFKGGKGGKQASEEKKQDDDEDTGPKPMLPYSSMFILSPTNPVRRGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVDEHSMRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCSDDSKYTEEECHGQYFVFEDGNLLPSSRKREWQPQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPNKRNSVKYKIWRIVVSTPFEYFIMILIVLNTLLLMMK 1132 HSP 2 Score: 177.563 bits (449), Expect = 1.332e-41 Identity = 200/852 (23.47%), Postives = 360/852 (42.25%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFN----SIYFVTLIVIGSFFMLNLVLGVLSGEFAKERE---RVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDE---PDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH-YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF-------------------NFQDGT--PPS----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIE-------MSIFYIVFFIVF-PFFFVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G S+YF+ L++ G++ +LN+ L + A +E E + K ++ Q+LE+E+ A + TD + E + + K K GK S E DDDE P L +S F N VR V ++F +F+++++ +++ +A E D+ + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F F+DG P S +++ A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + ++ + LE + N R N ++ I IV F+ FIM++I+++++ L + Q + L Y + FTG+F +E +LKI+ GF ++ +D WN D + V ++V G+N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F AE E+ F S+ ++ LF T E WP I+ + + D + + ++E ++ Y V FI F F +N+FVA+I+ F
Sbjct: 612 TNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQMQELEKEME--------------ALQMPTDGSPPKV-EICPPSPTRNFKGGKGGKQASEEKKQDDDEDTGPKPMLPYSSMFILSPTNP-------------VRRGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVDEHSMRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCSDDSKYTEEECHGQYFVFEDGNLLPSSRKREWQPQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCIDFTIQAR-----------PLERYMPNKR-------------------------NSVKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKYHRQGDLYQK-TLKYMNICFTGMFTIECILKIVAFGF----RNFFKDPWNTFDFITVIGSIVDALV--VEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL--------EAESAINEHNNFQSF---------------------IQGLMLLFRCATGEAWPNIMLSCIKGRPCD---LKVQKVETEGCGSNLAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1353 HSP 3 Score: 132.494 bits (332), Expect = 1.750e-27 Identity = 96/322 (29.81%), Postives = 159/322 (49.38%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-----------FVFSSYNRPPMVFPRL--WQKQK---FHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
SS+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + + F GIF VE LKI+ LGFILH GSY R++WNI+D VV + QG + +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE+ ++ P V + W+ FDN+ AMLT+F T EGW AIL +++ +T+ +++I ++ FF +N+ + ++ F ++ E
Sbjct: 21 SSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEHTEVYFLGIFCVEASLKILALGFILHRGSYLRNIWNIMDFFVVVTGFITMFTQGINLDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGILHKTCYSIEDLNQIVKEGEFPSPCNTDNLTEAPLGAHVCDPSVSACMDRWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFN------------WVYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 327 HSP 4 Score: 77.0258 bits (188), Expect = 1.981e-10 Identity = 78/334 (23.35%), Postives = 141/334 (42.22%), Query Frame = 0
Query: 249 KRVRFFIRSTVKQQW--FYWFVIILVFFNTVCVAVEHY---DQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFN------------FQDGTPPS------------------------------------NFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQL 525
K RF I +W F + V++ + N V +A+E + L L + E FLG+F E +++ ALG Y + +N D V+V F M+T + + L LRA+R+LR K+ SL+ ++ S++ +M ++ + L+ I+IFA++G++ + G + ++G PS +F+ A+LTVFQ +T E W ++Y ++ G +YFI L + G++ +LN+ L + A +E + EN ++ +E++L
Sbjct: 34 KHTRFII------EWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEHTEVYFLGIFCVEASLKILALGFILHRGSYLRNIWNIMDFFVVVTG-FITMFTQGINL--DMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGILHKTCYSIEDLNQIVKEGEFPSPCNTDNLTEAPLGAHVCDPSVSACMDRWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALG----STFNWVYFIPLIVLGSFFMLNLVLGVLSGEFAKERE------KVENRQSFLKLRRAQQLEREL 348
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|766923154|ref|XP_011506186.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 [Ceratosolen solmsi marchali]) HSP 1 Score: 1295.41 bits (3351), Expect = 0.000e+0 Identity = 665/932 (71.35%), Postives = 772/932 (82.83%), Query Frame = 0
Query: 24 DETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRA-ANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL------------RNGRSAY------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ------GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
D V +G + PC++D+ ++AP GA+ CN+N S C ++WEGPN GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G++FN IYF+ LIVIGSFFMLNLVLGVLSGEFAKERE+VENRQ FLK+RR QQLERELNGYV+WICKAEEVILAEERTT+EE+ HIMEAR++A A KRKK++ LG S+ST+T++D+ E D+ S+AS +++++NQG C + FWR EKR RF+IR TVK QWFYWFVI+LVFFNTVCVAVEHY QP+WLT+FL+Y EFVFLGLF+ EMV++MYALGPRIYFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLL+SMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGI+SQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQELTAAE + E + + + +EK+++ + S ++E+ + G+ A D+D KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV + + RN +L YFDY FTG+F +EM+LKIIDLG ILHPGSY R+ WN +DA+VV CA V+F F G S +NLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+CTD SK E+CQG +FVF P R W+ Q FH+DNV AMLTLFAVQT EGWP ILQNSMAATYE++GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+V+STPFEYFIMILIVLNT+LLMMK
Sbjct: 211 DLIVKDGEQPNPCNTDNESEAPAGAHVCNANTSKCSDRWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWIYFIPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQTFLKMRRHQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRKAEAAKRKKLKNLGTSKSTDTEEDEGEDDQEDGFSRASYLKSKVKNQGSCAE-FWRNEKRFRFWIRHTVKTQWFYWFVIVLVFFNTVCVAVEHYGQPEWLTEFLFYTEFVFLGLFMCEMVIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSAV--KSGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIQSQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQE---EEDKEKQIQELEKEMESLQKPNDGSAPKVEISPPSPTANFKGGKGGKQASEEKKQDDEDDSGPKPMLPYSSMFILSPTNPVRRGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVNDSNPRNTILNYFDYAFTGVFTIEMILKIIDLGVILHPGSYLREFWNFMDAIVVVCAAVSFAFDMTSGDGGGSASKNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKYTKEDCQGWFFVFEENELHPEAKERKWESQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYENKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSMKYKIWRIVMSTPFEYFIMILIVLNTLLLMMK 1136 HSP 2 Score: 160.999 bits (406), Expect = 2.411e-36 Identity = 191/855 (22.34%), Postives = 353/855 (41.29%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNS----FNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQ---QQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH-YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEV---MWTNCVEMAGSFGLSVLRALRLLRIF---KVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--------------------QDGTPPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFR-------IEMSIFYIVFFIVF-PFFFVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y + G S+YF+ L++ G++ +LN+ L + A +E + + ++ Q+LE+E+ L + + I A K K K + D+DD P L +S F N VR V ++F +F+++++ +++ +A E + L Y ++ F G+F EM++++ LG P Y +N D IV+V + M + + S LS +++LR+LR+ K K L+ + +++S++++I++L + LF IFA++ +QLF G F + ++ P +++ +A+LT+F V TGE W +++ + E++G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ Y + N ++ I IV F+ FIM++I+++++ L + Q +N L Y + FTG+F VE ++KI G ++ +D WN D + V ++V A + G+N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F +K+ N +F + ++ LF T E WP N M A D+ P + ++ Y V FI F F +N+FVA+I+ F
Sbjct: 612 TNFNTFPIALLTVFQILTGEDWNEVMYQGIQSQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQEEEDKEKQIQELEKEMES------------LQKPNDGSAPKVEISPPSPTANFKGGKGGKQASEEKKQDDEDDSGPKPMLPYSSMFILSPTNP-------------VRRGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVNDSNPRNTILNYFDYAFTGVFTIEMILKIIDLGVILHPGSYLREFWNFMDAIVVVCAAVSFAFDMTSGDGGGSASKNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKYTKEDCQGWFFVFEENELHPEAKERKWESQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYENKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPLERY----MPKERNSMKYKIWRIVMSTPFEYFIMILIVLNTLLLMMKYHRQSDLYKN-TLKYMNMCFTGMFTVECIMKIAAFGV----RNFFKDPWNTFDFITVIGSIVDALVIE---FGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGN--IALVEKNSINKHN---------------------------NFQSFIQGLMLLFRCATGEAWP----NIMLACIADQPCDPRSKESGDKCGSNLAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1356 HSP 3 Score: 137.502 bits (345), Expect = 4.926e-29 Identity = 96/326 (29.45%), Postives = 160/326 (49.08%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKF-----------FFCTDKSKPN-------AEECQGEYFVFSSYNRPPMVFPRLWQKQK---FHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
SS+FILS N IR I+ +P F+ +++ II + + LA E+ P Q+++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG + +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G D +PN +E G + ++ ++ W+ FDN+ AMLT+F T EGW AIL +++ +T+ I++I ++ FF +N+ + ++ F ++ E
Sbjct: 20 SSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPCQDKTTLAKKLETTEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFPQGINLDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIENADLIVKDGEQPNPCNTDNESEAPAGAHVCNANTSK----CSDRWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFN------------WIYFIPLIVIGSFFMLNLVLGVLSGEFAKERE 326
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|805757715|ref|XP_012147114.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X5 [Megachile rotundata]) HSP 1 Score: 1291.95 bits (3342), Expect = 0.000e+0 Identity = 665/924 (71.97%), Postives = 778/924 (84.20%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL---------RNGRSAY---------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++N+S C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQL+RELN Y+DWICKAEEVILAEERTT+EE+ HI+E R+RAA K+KK++ LGKS+ST+T++++ + D+ LS++S F++R + +G C Q FWR EKR RF+IRS+VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAE++ + + ++ +EK+++ + + + ++E+ ++G+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFV+ P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 216 VKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLDRELNCYLDWICKAEEVILAEERTTEEEKMHILEVRRRAAAKKKKLKNLGKSKSTDTEEEEGDDDQDDRLSRSSYFKSREKEEGTCVQ-FWRAEKRFRFWIRSSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFESSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEK---VEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1133 HSP 2 Score: 132.494 bits (332), Expect = 1.786e-27 Identity = 97/322 (30.12%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-FVFSSYNRPPMVFPR------------LWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRNINVIVKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 328
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|805757722|ref|XP_012147131.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X7 [Megachile rotundata]) HSP 1 Score: 1290.79 bits (3339), Expect = 0.000e+0 Identity = 665/924 (71.97%), Postives = 778/924 (84.20%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL---------RNGRSAY---------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++N+S C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQL+RELN Y+DWICKAEEVILAEERTT+EE+ HI+E R+RAA K+KK++ LGKS+ST+T++++ + D+ LS++S F++R + +G C Q FWR EKR RF+IRS+VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAE++ + + ++ +EK+++ + + + ++E+ ++G+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFV+ P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 340 VKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLDRELNCYLDWICKAEEVILAEERTTEEEKMHILEVRRRAAAKKKKLKNLGKSKSTDTEEEEGDDDQDDRLSRSSYFKSREKEEGTCVQ-FWRAEKRFRFWIRSSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFESSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEK---VEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1257 HSP 2 Score: 167.548 bits (423), Expect = 2.262e-38 Identity = 192/862 (22.27%), Postives = 352/862 (40.84%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFN----SIYFVTLIVIGSFFMLNLVLGVLSGEFAKERE---RVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH---YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--------------------QDGTPPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-----AFG--FQGSSTGQNLSTIKS-------LRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFF----FVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G S+YF+ L++ G++ +LN+ L + A +E + + K ++ Q+LE+E+ L + + + + I K K K + +DDD P L +S F N VR V ++F +F+++++ +++ +A E D P+ + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F + +D P +++ A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ ++ N ++ I IV F+ FIM +I+++++ L + Q + +N L Y + FTG+F VE +LKI G ++ +D WN D + V ++V FG F +G L K LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F + N P + +F + A ++ LF T E WP I+ + + D S +F+ F FF +N+FVA+I+ F
Sbjct: 737 TNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEKVEDKEKQAQELEKEIES------------LQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNP-------------VRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPR--NEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPL----ERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGV----RNFFKDAWNTFDFITVIGSIVDALVIEFGERFSSMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG------------------------NISNDPGTAI-----DEHNNFQSFFAGLMLLFRCATGEAWPNIMLSCIKGRPCDEKAGKQTEECGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1493 HSP 3 Score: 132.109 bits (331), Expect = 2.033e-27 Identity = 97/322 (30.12%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-FVFSSYNRPPMVFP------------RLWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 146 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRNINVIVKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 452
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|805757709|ref|XP_012147094.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X3 [Megachile rotundata]) HSP 1 Score: 1290.79 bits (3339), Expect = 0.000e+0 Identity = 665/924 (71.97%), Postives = 778/924 (84.20%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL---------RNGRSAY---------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++N+S C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQL+RELN Y+DWICKAEEVILAEERTT+EE+ HI+E R+RAA K+KK++ LGKS+ST+T++++ + D+ LS++S F++R + +G C Q FWR EKR RF+IRS+VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAE++ + + ++ +EK+++ + + + ++E+ ++G+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFV+ P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 216 VKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLDRELNCYLDWICKAEEVILAEERTTEEEKMHILEVRRRAAAKKKKLKNLGKSKSTDTEEEEGDDDQDDRLSRSSYFKSREKEEGTCVQ-FWRAEKRFRFWIRSSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFESSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEK---VEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1133 HSP 2 Score: 132.109 bits (331), Expect = 1.900e-27 Identity = 97/322 (30.12%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-FVFSSYNRPPMVFPR------------LWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRNINVIVKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 328 HSP 3 Score: 73.1738 bits (178), Expect = 2.506e-9 Identity = 53/220 (24.09%), Postives = 108/220 (49.09%), Query Frame = 0
Query: 298 EFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWT------NCVEMAGSFG--------LSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG-AFNFQDG-TPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGI-----ESQGGHRS----GMIYCLYFIILTLFGNYTLLNVFLAIAVDN 492
E+ +GL + V+ M + +F+ ++N FD I ++ S+ + + + + G G + LR R R+ K+ + ++R L+ + + S +++ + L+ + I+A++GMQ+FG A + + T +NF +F L+ +F+ TGE W +M I + + G ++ I YF+ F ++ +LN+F+A+ +DN
Sbjct: 1116 EYFIMGLIVLNTVLLMM----KNFFKDAWNTFDFITVIGSIVDALVIEFGERFSSMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDAETAITKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCIKGRPCDEKAGKQTEECGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDN 1331
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|805757706|ref|XP_012147083.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X2 [Megachile rotundata]) HSP 1 Score: 1290.02 bits (3337), Expect = 0.000e+0 Identity = 665/924 (71.97%), Postives = 778/924 (84.20%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL---------RNGRSAY---------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++N+S C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQL+RELN Y+DWICKAEEVILAEERTT+EE+ HI+E R+RAA K+KK++ LGKS+ST+T++++ + D+ LS++S F++R + +G C Q FWR EKR RF+IRS+VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAE++ + + ++ +EK+++ + + + ++E+ ++G+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFV+ P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 216 VKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLDRELNCYLDWICKAEEVILAEERTTEEEKMHILEVRRRAAAKKKKLKNLGKSKSTDTEEEEGDDDQDDRLSRSSYFKSREKEEGTCVQ-FWRAEKRFRFWIRSSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFESSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEK---VEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1133 HSP 2 Score: 168.318 bits (425), Expect = 1.107e-38 Identity = 189/849 (22.26%), Postives = 351/849 (41.34%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNS----FNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERE---RVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH---YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--------------------QDGTPPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFF----FVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G S+YF+ L++ G++ +LN+ L + A +E + + K ++ Q+LE+E+ L + + + + I K K K + +DDD P L +S F N VR V ++F +F+++++ +++ +A E D P+ + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F + +D P +++ A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ ++ N ++ I IV F+ FIM +I+++++ L + Q + +N L Y + FTG+F VE +LKI G ++ +D WN D + V ++V A + G+N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F +AE ++ F S+ ++ LF T E WP I+ + + D S +F+ F FF +N+FVA+I+ F
Sbjct: 613 TNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEKVEDKEKQAQELEKEIES------------LQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNP-------------VRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPR--NEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPL----ERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGV----RNFFKDAWNTFDFITVIGSIVDALVIE---FGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL--------DAETAITKHNNFQSF---------------------IQGLMLLFRCATGEAWPNIMLSCIKGRPCDEKAGKQTEECGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1353 HSP 3 Score: 132.109 bits (331), Expect = 2.010e-27 Identity = 97/322 (30.12%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-FVFSSYNRPPMVFPR------------LWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRNINVIVKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 328
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|805757703|ref|XP_012147077.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X1 [Megachile rotundata]) HSP 1 Score: 1290.02 bits (3337), Expect = 0.000e+0 Identity = 665/924 (71.97%), Postives = 778/924 (84.20%), Query Frame = 0
Query: 27 VTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEG--LSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMEL---------RNGRSAY---------DDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ--GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
V EG + PC++D+ ++AP GA+ C++N+S C++ WEGPN+GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQL+RELN Y+DWICKAEEVILAEERTT+EE+ HI+E R+RAA K+KK++ LGKS+ST+T++++ + D+ LS++S F++R + +G C Q FWR EKR RF+IRS+VK Q FYWFVI+LVFFNTVCVAVEHY QPQWLTDFLY+AEFVFL LF+ EM +++YALGPR YFESSFNRFDC+VI S+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGG FNF+ GTPP+NFNTF IALLTVFQ+LTGEDWNEVMYQGIESQGGH+ GMIY LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AAE++ + + ++ +EK+++ + + + ++E+ ++G+ +DD KP+LPYSSMFILS +NP+R H +V +FD FIMV+I +SSIALAAEDPV E S RN VL YFDY FTG+F VEM+LKIIDLG ILHPGSY R+ WNI+DAVVV CA V+F F GSS GQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF+C+D+SK ++CQG+YFV+ P R WQ Q FH+DNV AAMLTLFAVQT EGWP ILQNSMAATYED+GP+ FRIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIM LIVLNT+LLMMK
Sbjct: 216 VKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLDRELNCYLDWICKAEEVILAEERTTEEEKMHILEVRRRAAAKKKKLKNLGKSKSTDTEEEEGDDDQDDRLSRSSYFKSREKEEGTCVQ-FWRAEKRFRFWIRSSVKSQKFYWFVIVLVFFNTVCVAVEHYGQPQWLTDFLYFAEFVFLALFMLEMFIKVYALGPRTYFESSFNRFDCVVISGSIFEVIWSEV--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFESGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEK---VEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMK 1133 HSP 2 Score: 167.162 bits (422), Expect = 2.918e-38 Identity = 192/862 (22.27%), Postives = 352/862 (40.84%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFN----SIYFVTLIVIGSFFMLNLVLGVLSGEFAKERE---RVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH---YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGS--FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNF--------------------QDGTPPS-----------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-----AFG--FQGSSTGQNLSTIKS-------LRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFF----FVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G S+YF+ L++ G++ +LN+ L + A +E + + K ++ Q+LE+E+ L + + + + I K K K + +DDD P L +S F N VR V ++F +F+++++ +++ +A E D P+ + L Y ++ F G+F EM++++ LG P Y +N D +V++ + + AG + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F + +D P +++ A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ ++ N ++ I IV F+ FIM +I+++++ L + Q + +N L Y + FTG+F VE +LKI G ++ +D WN D + V ++V FG F +G L K LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F +AE ++ F S+ ++ LF T E WP I+ + + D S +F+ F FF +N+FVA+I+ F
Sbjct: 613 TNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEDEEKVEDKEKQAQELEKEIES------------LQKPKDGNAPKVEICPPSPTQNFKDGKGGKQSSEEKKQDEDDDTGPKPMLPYSSMFILSPTNP-------------VRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPR--NEVLNYFDYAFTGVFTVEMILKIIDLGVILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTEQDCQGQYFVYEEDAMLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPL----ERYMPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGV----RNFFKDAWNTFDFITVIGSIVDALVIEFGERFSSMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL--------DAETAITKHNNFQSF---------------------IQGLMLLFRCATGEAWPNIMLSCIKGRPCDEKAGKQTEECGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1369 HSP 3 Score: 132.109 bits (331), Expect = 2.034e-27 Identity = 97/322 (30.12%), Postives = 157/322 (48.76%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFF-FCTDKSKPNAEECQGEY-FVFSSYNRPPMVFPR------------LWQKQKF---HFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS N IR I+ +P F+ +++ II + + LA E+ P +++ L + F GIF VE LKI+ LGF+LH GSY R++WNI+D VV + QG +L T LR +RVLRPLK + +P L+ V ++ ++ + I ++ L IFA+I ++ ++G C N +GE ++ N+ F W+ F FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRT---LRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYSIRNINVIVKEGEQPSPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 328
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Match: gi|1070588487|ref|XP_018406731.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 [Cyphomyrmex costatus]) HSP 1 Score: 1289.25 bits (3335), Expect = 0.000e+0 Identity = 660/925 (71.35%), Postives = 765/925 (82.70%), Query Frame = 0
Query: 24 DETVTEGNKNVPCHSDDPTKAPVGAYTCNSNISICLEKWEGPNYGITSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERERVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEHYDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALGPRIYFESSFNRFDCIVIVASVFEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAFNFQDGTPPSNFNTFAIALLTVFQVLTGEDWNEVMYQGIESQGGHRSGMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAA----------------ENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQKPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALVAFGFQ----GSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKACIDFAIQARPLERYMPKERDSFKYKIWRVVVSTPFEYFIMILIVLNTILLMMK 928
+E V EG+ PC +D+ +AP GA+ C++N+S C++ WEGPN GITSFDNIGFAMLTVFQC+TMEGWT+ILYWTNDA+G+++N IYF+ LIV+GSFFMLNLVLGVLSGEFAKERE+VENRQ+FLKLRRQQQLERELN Y++WICKAEEVILAEERTT+EE+ HI+EAR+RAA K++K++ LGKS+ST+T++++ E E +AS F+++++ QG C Q FWR EKR RF+IR++VK Q FYWFVI+LVFFNTVCVAVEHY+QP+WLTDFLYYAE+VFLGLF+ EM +++YALGPR YFESSFNRFDCIVI AS+FEV+W+ +GSFGLSVLRALRLLRIFKVTKYW SLRNLVISLLSSMRSI+SLLFLLFLFILIFALLGMQLFGG FNF DGTPP+NFNTF IALLTVFQ+LTGEDWNEVMY+GIESQG M + LYFI+L LFGNYTLLNVFLAIAVDNLANAQEL+AA E + E+ + ++ E++L F + + +E + R +DD KP+LPYSSMFILS +NPIR H IV +FD FIMV+I +SSIALAAEDPV E++ RN +L YFDY FTG+F +EMVLKIIDLG ILHPGSY R+ WNI+DAVVV CA+V+ F S+ QNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNV NILIVY+LFQFIFAVIAVQLFNGKFF CTD SK E+C G +FVF P + PR WQ Q FH+DNV AMLTLFAVQT EGWP ILQNSMAATYED+GP+ +RIEMSIFYIV+FIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQK+CIDF IQARPLERYMPKER+S KYKIWR+VVSTPFEYFIMILIVLNT+LLMMK
Sbjct: 213 NEIVKEGDMPSPCSADNRNEAPPGAHVCDANVSTCMDHWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYNWIYFIPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQSFLKLRRQQQLERELNCYLNWICKAEEVILAEERTTEEEKMHILEARRRAAAKKRKLKNLGKSKSTDTEEEEGEDGEDDGEASYFKSKVKKQGTCLQ-FWRAEKRFRFWIRNSVKSQQFYWFVIVLVFFNTVCVAVEHYNQPKWLTDFLYYAEYVFLGLFMMEMFIKVYALGPRTYFESSFNRFDCIVISASIFEVIWSKL--KSGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIVSLLFLLFLFILIFALLGMQLFGGQFNFDDGTPPTNFNTFPIALLTVFQILTGEDWNEVMYKGIESQG-----MAHSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEQQEEDKEKQMQELEKEIESLQRRGDAPRPQSGERRLIF---SSRDGRIETQTSEERRQDEDDDTGPKPMLPYSSMFILSPTNPIRRGAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEKASRNQILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSIAFDILGSSSTASQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFHCTDDSKYTQEDCNGHFFVFEEGKLQPELKPRKWQSQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNYRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIQARPLERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMK 1126 HSP 2 Score: 176.407 bits (446), Expect = 3.509e-41 Identity = 195/850 (22.94%), Postives = 362/850 (42.59%), Query Frame = 0
Query: 70 TSFDNIGFAMLTVFQCVTMEGWTSILYWTNDAMGNSFNSIYFVTLIVIGSFFMLNLVLGVLSGEFAKERE---RVENRQAFLKLRRQQQLERELNGYVDWICKAEEVILAEERTTDEERSHIMEARKRAANKRKKMQKLGKSRSTETDDDDDEPDEGLSQASAFRTRLRNQGGCCQQFWRCEKRVRFFIRSTVKQQWFYWFVIILVFFNTVCVAVEH-YDQPQWLTDFLYYAEFVFLGLFITEMVVRMYALG----PRIYFESSFNRFDCIVIVASV----FEVMWTNCVEMAGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGAF------------------------NFQDGTPPS-------NFNTFAIALLTVFQVLTGEDWNEVMYQGI----ESQGGHRS-----GMIYCLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENEDQNETTAASRNMEKQLQFMHGTQKLSELEMELRNGRSAYDDDIFVQ-KPILPYSSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAEDPVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILDAVVVTCALV-AFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFFCTDKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAILQNSMAATYEDRGPVPLFRIE--------MSIFYIVFFIVF-PFFFVNIFVALIIITF 856
T+F+ A+LTVFQ +T E W ++Y ++ G + +S+YF+ L++ G++ +LN+ L + A +E E +Q K ++ Q+LE+E+ +R D R E R +++ +++ + +DDD P L +S F N +R V ++F +F+++++ +++ +A E + L Y ++ F G+F EMV+++ LG P Y +N D +V++ ++ F+++ ++ + LR LR+LR K K L+ + +++S++++I++L + LF IFA++ +QLF G F Q P +++ +A+LT+F V TGE W +++ + E +G ++ + Y +YFI+ F +N+F+A+ + QE AE Q D+N+ + D +Q +P+ ++ N I+ I IV F+ FIM++I+++++ L + Q S +N L Y + FTG+F VE VLKI G ++ +D WN D + V ++V A + G+N + LR+ R R +K +++ ++ + V S K + + ++ + FI+A+I +Q+F G D S + +N +F + ++ LF T E WP N M + + R P + ++ Y V FI F F +N+FVA+I+ F
Sbjct: 611 TNFNTFPIALLTVFQILTGEDWNEVMYKGIESQGMA-HSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAEEEQQEEDKEKQMQELEKEIESL--------------QRRGDAPRPQSGERRLIFSSRDGRIETQTSEERRQDEDDDTGPKPMLPYSSMFILSPTNP-------------IRRGAHWIVNLRYFDFFIMVVISLSSIALAAEDPVSEKASRNQILNYFDYAFTGVFTIEMVLKIIDLGIILHPGSYLREFWNIMDAVVVICAMVSIAFDILGSSSTASQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFHCTDDSKYTQEDCNGHFFVFEEGKLQPELKPRKWQSQSFHYDNVMVAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNYRIEMSIFYIVYFIVFPFF----FVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCI---------------------------------DFTIQARPL----ERYMPKERNSIKYKIWRIVVSTPFEYFIMILIVLNTLLLMMKFHRQSDSYKN-TLKYMNMCFTGMFTVECVLKIAAFGV----KNFFKDAWNTFDFITVIGSIVDALVIE---FGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF-GNIALDADTS-------------ITKHN---------------NFQSFIQGLMLLFRCATGEAWP----NIMLSCVKGRSCDPKAEKDEQDSCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1347 HSP 3 Score: 124.79 bits (312), Expect = 3.844e-25 Identity = 92/330 (27.88%), Postives = 156/330 (47.27%), Query Frame = 0
Query: 562 SSMFILSSSNPIRIFIHGIVTFPFFDTFIMVIIIMSSIALAAED--PVQEQSERNHVLTYFDYVFTGIFAVEMVLKIIDLGFILHPGSYCRDVWNILD-AVVVTCALVAFGFQGSSTGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVFNILIVYLLFQFIFAVIAVQLFNGKFFF--------------------CT----DKSKPNAEECQGEYFVFSSYNRPPMVFPRLWQKQKFHFDNVAAAMLTLFAVQTTEGWPAIL---QNSMAATYEDRGPVPLFRIEMSIFYIVFFIVFPFFFVNIFVALIIITFQEQGE 861
+S+FILS +N +R + I+ +P F+ +++ II + + LA E+ P Q+++ L + F IF E LKII LGF+LH GSY R++WNI+D VVVT ++ F ++ LR RVLRPLK + ++P L+ V ++ ++ + I ++ L IFA+I ++ ++G C+ +++ P A C + P FDN+ AMLT+F T EGW AIL +++ +TY I++I ++ FF +N+ + ++ F ++ E
Sbjct: 23 TSLFILSENNWLRRNVRFIIEWPPFEYGVLLTIIANCVVLALEEHLPRQDKTILAQKLESTEIYFLTIFCAEASLKIIALGFVLHRGSYLRNIWNIMDFFVVVTGSMTVFAETNVEVD-----LRMLRSFRVLRPLKLVSKIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGTLHKTCYNIMDINEIVKEGDMPSPCSADNRNEAPPGAHVCDANVSTCMDHWEGP-------NSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------------WIYFIPLIVLGSFFMLNLVLGVLSGEFAKERE 328 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_333049 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 17
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BLAST of hypothetical protein DAPPUDRAFT_333049 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_333049 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1237_size53912:388..11636- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1237_size53912-snap-gene-0.7 ID=maker-scaffold1237_size53912-snap-gene-0.7|Name=hypothetical protein DAPPUDRAFT_333049|organism=Tigriopus kingsejongensis|type=gene|length=11249bp|location=Sequence derived from alignment at scaffold1237_size53912:388..11636- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_333049' has the following synonyms
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