BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000012345 (supercontig:LSalAtl2s:LSalAtl2s900:155793:157148:-1 gene:EMLSAG00000012345 transcript:EMLSAT00000012345 description:"maker-LSalAtl2s900-snap-gene-0.28")

HSP 1 Score: 152.14 bits (383), Expect = 3.373e-42
Identity = 89/304 (29.28%), Postives = 155/304 (50.99%), Query Frame = 0

Query:  127 AKFQDLGCMLQPKDDAIDQGSLCGR-SDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISANE-RPYYFQPDE--YYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDF--VYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLG 424
              ++ L C  + ++    +   CG  S   ++I+IG+   D  F T +  C  +A+  + ++ HT+   ++       ++ RP YF+     +     +  Y    QK+   ++  +Q   + +     SFY ARGHL+P+GDF  ++ S  DA+++++NVAP WQ  N GNW  LE + R +V        VY+GT+G+ +L + NGVK+ ++L  E   +PVPK  WK+L +      + FIG NNP++ + E     +C     +  L+   +  ++  G L+ C  + V K+   +PDLG
Sbjct:   72 TSWKTLTCTRKIRESTKTRLQPCGPISYSNVMIDIGW-TSDTNFTTQLSVCHNIADETTWFSNHTIQGSLSSFGTFEKDDSRPSYFKEGSQFFTKSSASTSYKLRTQKDKFIKIFGAQ---NDFFSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVLKLPNKNGVKIPIYL-GEDELIPVPKLIWKILFNPRREEAIVFIGSNNPFLEQIETVCPDLCEDTHWNDFLEHRRF--EVSKGYLYCCSYHSVKKVISWLPDLG 368          

BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000000465 (supercontig:LSalAtl2s:LSalAtl2s106:710470:724606:-1 gene:EMLSAG00000000465 transcript:EMLSAT00000000465 description:"maker-LSalAtl2s106-augustus-gene-7.8")

HSP 1 Score: 147.132 bits (370), Expect = 2.409e-38
Identity = 114/430 (26.51%), Postives = 188/430 (43.72%), Query Frame = 0

Query:   25 FAVLLVFRSVIGIASQSDCIIEPDKAWPAFPP-IFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCG---------RSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEI-----------TGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIR-------PDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDI-FYPNDIELGILFACQVNDVDKIFEEIPDLGE 425
            +  L + + +IG  ++ DC I  D       P I ++  +Q I+P      + R IT  +GE + + C  S           S +          A      + K + L C  +       +   C            D+ +LI +GF    + ++ L+  C       +I+  HT+  E            T R+NI  ++  Y F        ++N  Y++  Q +TI +LL    +     R          + YFA+GHLSP+  F+Y + QD ++++ NVAPQ+Q FN  NW  +ES  R +  +    L+VY+GT  I +L +      ++ + P    +P P YYWKVL+D  ++  +AFIG+NNPY   +      ICT + +  + DD+ FY    E G     +V+ +      IPDL +
Sbjct:    7 YLTLFLLKFLIGTIAKEDCKINLDSRTGNHQPFILKEKTNQVIYP-----KESRIITIGDGENIVIDCHGSKLSKATHYGIPSGLSKISLSCDNGAFRNSPKIVKVEILSCTSKVYPQLERKSVKCSPVGADDRLTDLDDLVLINVGFNF-SSSYSPLMNICHDEKVYGTIWTHHTIRGESINNRDRTIDRPTFRTNIGRSKIYYPFTTMT----QMNSQYSKSTQVKTIKKLLGIYTIMVDGKRVPIIDESRSGTHYFAKGHLSPDAAFIYSAEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKQNLEVYTGTASILKLPNKQSQPTEIKMFPSLKYVPAPMYYWKVLYDPEANEAIAFIGLNNPY---ERKAHNHICTNICAQTVFDDVDFY--KFEAGYTMCXEVSQLRMSISSIPDLSK 421          

HSP 2 Score: 129.413 bits (324), Expect = 2.291e-32
Identity = 74/215 (34.42%), Postives = 117/215 (54.42%), Query Frame = 0

Query:  219 EINDFYTQVMQKETIAELLQSQAL-ADQYIRP--DDSF----YFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDI-FYPNDIELGILFACQVNDVDKIFEEIPDLGE 425
            ++N  Y++  Q +TI  L  + ++  D    P  D+S     YFA+GHLSP+  F+Y   QD ++++ NVAPQ+Q FN  NW  +ES  R +  +    L+VY+GT  I  L +     +++ L  +   +P P YYWKVL+D  ++  +AFIG+NNPY   +      IC+ + +  + DD+ FY    E G    C+V+ +      IPDL +
Sbjct:  858 QMNSQYSKSTQVKTIETLFGNNSIIVDGKEIPIIDESRSGTNYFAKGHLSPDAAFIYSVEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKRNLEVYTGTASILNLLNEQCKPINIELFSDRQYVPAPMYYWKVLYDPEANEAIAFIGLNNPY---ERKAHNHICSNICAQTVFDDVDFY--KFEAGYTMCCEVSQLRMSISSIPDLSK 1067          

HSP 3 Score: 129.413 bits (324), Expect = 2.291e-32
Identity = 124/416 (29.81%), Postives = 192/416 (46.15%), Query Frame = 0

Query:   39 SQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFV-NGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADG--EEDIVAKFQDLGCMLQPKDD-AIDQGSLCGRSDEGL-----------LIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPE-ITGRSNISANERPYYF----QPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYI-RPDDS--FYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPL-ESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            +++ C I  DK      P+   + S F++PT      D +IT V  G  +EL+C +S   NF   +      +   +   T D    E +     D  C  + +    I + S C  S EG+            I +G+  R   +   +E C+      +++ KH +  + I  R   S   RP +          +G    +  T+   K +     Q+ ++ DQ I  P  S  FY A+GHLSP+  FVY   Q+ +++F+NVAPQ+Q FN GNW  LE A RD  + + + L VY+GT+ I +L      K    L  +F  +PVP+Y+WKVLHD A    VAF+G NN      +     ICT + +  P +D  +    +  G ++ C V D++K     PDL + SLL+
Sbjct: 1086 TRNGCKILLDKLPEKNTPLITSNGS-FLYPT--YIKDDARITLVPQGSTVELNCHRSR-GNFLLYKEERVSKIESVKLTCTNDKLYTEGMEVNPADYKCSSKNQPSLIITRNSKC--SPEGIDKRKTDLERITHISLGWNFRSG-YIEQVEICIDELFYGTLWTKHYVEGQNIEMRDKYSG--RPAFIVDETGKKRLFGKRSTNQITKAYAKNS-----QNTSIYDQSIMNPSKSSKFYLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQ----KQIFLLEKKF--IPVPRYFWKVLHDPARKKAVAFVGYNN---VLRKTSPKPICTDVCDQIPWVD--WERESLFKGYMYCCNVEDLNKAISYSPDL-DASLLI 1475          

HSP 4 Score: 120.168 bits (300), Expect = 2.297e-29
Identity = 115/424 (27.12%), Postives = 197/424 (46.46%), Query Frame = 0

Query:   39 SQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDR-KITFV-NGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADG--EEDIVAKFQDLGCMLQPKDD-AIDQGSLCG-------RSDEGLL--IEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRS---NISANERPYYFQPD----EYYGY----EINDFYTQVMQKETIAELLQSQALADQYIRP------DDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPL-ESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            +++ C I  DK      P+   + S F++PT   Y +D  +IT V  G  +EL+C +S   NF   +      +   +   T D    E +     D  C  + +    I + S C        ++D G +  I +G+  R   F   +E C+      +++ KH     + G+S   +   ++RP +   +      +G     +I   Y +  QK+TI E+     +   Y  P        + + A+GHLSP+  FVY   Q+ +++F+N APQ+Q FN GNW  LE A RD  E+  + L VY+GT+ I +  +     ++  L      +P P+Y+WKV+HD ++   VAF+G NN +    +     IC  + +  P +D  +  + +  G ++ C+V+D++K     P L + SLL 
Sbjct:  440 TRNGCKILLDKLPEKNTPLITSNGS-FLYPT---YIKDEARITLVPQGSTVELNCHRSR-GNFLLYKEERISKIKSVKLTCTNDKLYTEGMEVNPADYKCSSKNQPSLIITRNSKCSPEGIDKRKTDLGRITHISLGWNFRSG-FIEQVELCIDELFYGTLWTKHN----VVGKSIEFSDKDSDRPAFIVDETGQKRLFGKRSTNKITQAYAKKSQKKTIKEITGHTTI---YGLPMIETNRKGTLFMAKGHLSPDAAFVYDGEQEGTYFFVNAAPQYQSFNNGNWRALELAVRDLAEKLHSTLTVYTGTYEILEFYEKQIFLLEKKL------IPAPRYFWKVVHDPSTKKAVAFVGYNNVF---SKTSPKPICKDVCDQIPWVD--WERDSLYKGFMYCCEVDDLNKAISYSPVL-DASLLT 838          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1325253623|ref|XP_023321392.1| (uncharacterized protein LOC111696065 [Eurytemora affinis])

HSP 1 Score: 290.041 bits (741), Expect = 1.264e-90
Identity = 160/390 (41.03%), Postives = 239/390 (61.28%), Query Frame = 0

Query:   43 CIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTF--ENFPQSEFASAVCVGGDEFLV-TADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLL 429
            CI+  D A   +PPI  D    F+ P G   S  R I    GE +++SC  S+F  +N  +     A C+    F V T D E +    ++D+ C  QP+D+    GS CG S+E  LI IGFQ+ DN+       C     SR+++ KHTL+ EI+ + +   N RP  FQ D +Y Y+++  Y++  Q+E I++L+ SQ LA+QYI    + + ARGHL+PN DF++FS  D+SF+F+NVAPQWQ FNG NW  LE+  R+F+  R    ++Y+GTHGI +LADV G  VD+++  + N+LPVPK+YWK++ D    +GVA IGVNNP++TE     + +C PL++  IL+ +  P ++  G ++AC+V ++ K+ EEIP L ++ LL
Sbjct:   46 CILNIDGAL-QYPPILADLSGSFLLPEGDDIS--RNIPVPQGEVMQVSCPGSSFGFDNIWEDGPVYARCIEDSSFDVWTPDTEGEDRVFWEDMSCKSQPRDNTEILGS-CGPSEENTLIRIGFQIADNQTTPTFTVCFDQTISRTLWVKHTLWDEISAQDH--GNSRPD-FQKDSFYDYDVDHAYSRNAQREEISKLVGSQELAEQYIEESGNLFLARGHLAPNADFIFFSWMDSSFHFINVAPQWQTFNGLNWNSLENTVRNFIISRTFDAEIYTGTHGILELADVKGDMVDIYIY-DGNRLPVPKFYWKIVLDPLQETGVAVIGVNNPHLTELPASYI-LCPPLDNPTILEGVKEPENLYKGYIYACRVEELAKVVEEIPSLPKVDLL 426          

BLAST of duplex-specific nuclease vs. nr
Match: gi|398708950|gb|AFP19103.1| (duplex-specific nuclease [Scylla paramamosain])

HSP 1 Score: 229.18 bits (583), Expect = 3.195e-67
Identity = 138/410 (33.66%), Postives = 214/410 (52.20%), Query Frame = 0

Query:   25 FAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNE-FATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNG--VKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPL-ESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            F ++ +F + +     SDC+   DK +P +PP+  DS  +F+ P       +R +    GE + L+C     EN  Q E A A C+  +  L   + E D+ +    LGC    K+        CG  + G+L EIGF++   E F  ++  C   +   ++Y +H ++       +I ++ RP +     ++   ++  Y+Q  Q E + +LL  + LA Q I     FYFA+GH+SP+ DFV  ++QDA++Y++N  PQWQ FN GNW YLE ATRD  E++   L VYSG  G+ +L D+NG  V+V L L  E   +P P   WKV+HD  ++   A +GVNNP++T        +C  L  S P +D  F  +D+  G  + C V D+      +P+LGE+ LL 
Sbjct:   11 FVLITIFSACVYC---SDCVWNKDKDFPQYPPLILDSSFEFVLPVEE--DGNRVVRVAAGETVTLACPGDEIENLHQVE-AEARCLE-NGLLAIENSEWDMAS----LGCGDDVKEVIFRDQGACGAGNIGILHEIGFEILSAEKFKKVLRVCFDPSLETTLYTEHLIHGANIAAKDIDSS-RPSFKSSTGFFSVSMSTVYSQSSQLELMIKLLGDEDLAHQIINTHKQFYFAKGHMSPDADFVLMANQDATYYYINALPQWQAFNNGNWKYLEFATRDLAEKKRRDLRVYSGGWGVLELIDINGNPVEVFLGLAQEKKVVPAPAVTWKVVHDELTNCAAAIVGVNNPHLTS---APSTLCEDLCSSLPWID--FDVSDLGHGYTYCCSVADLRAAVPHVPELGEVCLLT 403          

BLAST of duplex-specific nuclease vs. nr
Match: gi|321474829|gb|EFX85793.1| (hypothetical protein DAPPUDRAFT_98405 [Daphnia pulex])

HSP 1 Score: 226.098 bits (575), Expect = 3.655e-66
Identity = 142/417 (34.05%), Postives = 225/417 (53.96%), Query Frame = 0

Query:   27 VLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSC-IKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISA----NERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ---LPVPKYYWKVLHDSASSSGVAFIGVNNPY---ITEDEIEALKICTPLESHPILDDIFYPN--DIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            +L+   S++G AS + C  + + + PA PP F D+  + I PT  + +  R IT    +Q+ ++C   S       ++F SA C      L    G +++   +  LGC    K+   + G+ C  S  G  IEIG+Q+ +  +   +  C   + + ++Y+ +T    I+G +NI+A    N RP + + + + G   +  Y+Q  Q  ++A ++ S  LA +YI    S+YFARGHL+P+GDF+    QDA++Y++N APQWQ FN GNW  LE   RD    R   L +Y+GT+ I  LAD+NGV+  ++L  + N    LP PKYYWKV+HD  +++  A IG+NNP+   +T  ++    +C  L        + +PN  DI  G +F C V  +  +    P+LG+LSLLV
Sbjct:    7 LLVALFSLLGTASAA-CTFDLNASSPANPPHFVDAFGKIIQPT--LQNGVRIITLDANQQITVACEGTSNLLTATSAQFNSATCSSAGSTLQV--GTQNL--GYSSLGCKTAVKETIKETGT-CMTS--GTQIEIGWQV-NTAYINQLTVCHVKSTANTLYSINT----ISG-ANIAADDKANLRPSFRKGNYFVGINPDTGYSQAGQNVSVANIVGSYDLAIKYIDVGKSYYFARGHLAPDGDFIDAGSQDATYYYINAAPQWQSFNNGNWKSLEFGVRDLAISRARDLVIYTGTYDILTLADINGVQKPIYLAKDANNNNVLPAPKYYWKVVHDPVTNTATAVIGINNPHLTTVTGADVFCADVCNQLTW------MTWPNRFDITRGYMFCCSVQSLRNVVAHAPNLGDLSLLV 401          

BLAST of duplex-specific nuclease vs. nr
Match: gi|326381239|gb|ADZ58187.1| (duplex-specific nuclease [Portunus pelagicus])

HSP 1 Score: 226.098 bits (575), Expect = 4.093e-66
Identity = 145/412 (35.19%), Postives = 212/412 (51.46%), Query Frame = 0

Query:   23 FCFAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNE-FATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNG--VKVDLWLRPEFNQLPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPL-ESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            F   VL+V  S  G    SDC+ + DK +P +PP+  DS  +F+ P     + +R +    GE + L+C     EN    E A A C+     +V     ED V     LGC    K+  +     CG  D G+L EIGF++   E F  ++  C   A   ++Y +H ++       +I ++ RP +     ++   ++  Y+Q  Q E + +LL  + LA Q I      YFA+GH+SP+ DFV   +QDA++Y++N  PQWQ FN GNW YLE ATRD  E++   L VYSG  G+ +L D+NG  V+V L L  E   +P P   WKV+HD  ++   A +GVNNP++T        +C  L  S P +D  F   D+E G  + C V D+      +PDLGE+ LL 
Sbjct:    8 FTHFVLIVLFSAFG--HCSDCMWDKDKDFPRYPPLILDSNYEFVLPVEE--NGNRVVRLALGEIVTLACPGDELENLHLVE-AKARCLEDGLLMV-----EDSVWDMASLGCGDDVKEMILRDQGACGAGDIGVLHEIGFEVLSAERFKKVLRVCFDPASETTLYTEHLIHGASIAAKDIDSS-RPSFKTSTGFFSVSMSSVYSQSSQLELMIKLLGDEDLAHQIIDTHKQLYFAKGHMSPDADFVIMDNQDATYYYINAVPQWQAFNNGNWKYLEFATRDLAEKKKRDLRVYSGGWGVLELDDINGNPVEVFLGLAQEKKVVPAPAVTWKVVHDEVTNCAAAIVGVNNPHLTS---APSTLCEDLCSSLPWID--FDVADLEHGYTYCCSVADLRAAVPHVPDLGEVCLLT 403          

BLAST of duplex-specific nuclease vs. nr
Match: gi|321473935|gb|EFX84901.1| (hypothetical protein DAPPUDRAFT_194191 [Daphnia pulex])

HSP 1 Score: 226.098 bits (575), Expect = 4.710e-66
Identity = 138/394 (35.03%), Postives = 212/394 (53.81%), Query Frame = 0

Query:   38 ASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIK-STFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYY-GYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ---LPVPKYYWKVLHDSASSSGVAFIGVNNPY--ITEDEIEALKICTPLESHPILDD-IFYPNDIELGILFACQVNDVDKIFEEIPDL 423
            ++++ C I  D + P FPP   D   + + P   I    R I    G+++ +SC+      N    +   A C      L  ADG E     + +L C++Q  +  ++QG+ C     G  I  G++    +F  L + C   A + + YA  T+Y   +  ++   N+RPYYF  D Y+ G  +N  YTQV Q +TIA ++ S+ LA QYI P+  +YFARGHL+P+ DF+  + QDAS+YFMN APQ+Q+FN GNW +LE   RD    R   L +Y+GT  +  LAD+NGV+  ++L  + N    +P PKYYWK++H+  S++  A IG+NNPY  +  ++I    +C  +   P ++D IF   +I  G  F C   ++ K     P+L
Sbjct:   26 SNKAGCAINLDTSSPTFPPHLIDENKKIVLPV--IEGTARIINLNEGQKVTVSCLGVGNLLNATGLQLNPATCAASSN-LQFADGTE---FSYDELTCLIQNHEVLVEQGT-CANG-PGTFIRNGWEFGA-DFIPLYDMCHDEALALNYYAIDTVYGR-SANADDKTNDRPYYFSQDVYFPGISVNTQYTQVEQTKTIAMIVGSEELAAQYIVPNTEYYFARGHLAPDADFIDAASQDASYYFMNTAPQFQIFNNGNWKWLEIGVRDTALARQLDLTIYTGTFSVTTLADINGVQQPIFLAFDANNNGLIPAPKYYWKLIHEPISNTATAVIGINNPYNAVMPEDILCPDVCDQI---PWVNDAIFQLTNIAKGYTFCCTAAELHKAISFAPNL 406          

BLAST of duplex-specific nuclease vs. nr
Match: gi|321475023|gb|EFX85987.1| (hypothetical protein DAPPUDRAFT_313567 [Daphnia pulex])

HSP 1 Score: 225.713 bits (574), Expect = 5.260e-66
Identity = 138/417 (33.09%), Postives = 214/417 (51.32%), Query Frame = 0

Query:   18 LTMVKFCFAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSC-IKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ---LPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            + + K   AV+L+   ++     + C    D + P +PP F DS +  I PT  + +  R IT    + + ++C   + F     ++F S  C      L    G +++   +  LGC    K+   + G+    S  G  IEIG+Q+ ++ +   +  C   +   ++YA HT+Y      ++  AN RP + + + + G ++   Y+Q  Q  T+++++ S  L  QYI    S+YFARGHL+P+GDF+    QDA++Y++N APQWQ FN GNW  LE+ TRD    R   + VY+GT     LADV GV+  ++L  + N    LP PKYYWKV+HD AS+   AFIG+NNP++T     A   CT + S           DI  G +F C    +  +    P+LG + LLV
Sbjct:    1 MAITKKLLAVVLLIACLVADPVSAVCTFNLDTSSPTYPPHFVDSSNNIIQPT--LQNGVRTITLAASQSITVACEGTNNFLMATNAQFNSGKCATAGTNLQI--GTQNL--GYSSLGCNAVVKETIKETGTC---STTGTKIEIGWQV-NSAYINQLTVCHVQSNDNTLYAIHTIYGANA-AADDKANARPSFRKGNYFVGVDVETAYSQAGQLVTVSDIVGSSTLGAQYIDTTKSYYFARGHLAPDGDFIDAGSQDATYYYINAAPQWQSFNNGNWKALEAGTRDLAISRVIDVVVYTGTFETLTLADVKGVQKPIYLAKDGNNNNVLPAPKYYWKVVHDPASNKATAFIGINNPHLTSVS-GADVFCTDVCSQVGWMSWSNRFDIPKGYMFCCTAAGLHNVVAHAPNLGSIGLLV 405          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1022766590|gb|KZS11634.1| (Uncharacterized protein APZ42_023543 [Daphnia magna])

HSP 1 Score: 224.172 bits (570), Expect = 2.103e-65
Identity = 139/422 (32.94%), Postives = 220/422 (52.13%), Query Frame = 0

Query:   25 FAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSC------IKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISA----NERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ---LPVPKYYWKVLHDSASSSGVAFIGVNNPY---ITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            F VL+    ++  ++ +DC    D + PA PP   DS  + I PT  + +  R IT    + + ++C      + +T       + A+A    G   L+   G +++  ++  LGC    K+   + G+    S  G  IEIG+Q+    F   +  C   + + ++Y+ +T    I+G +NI+A    N RP + +   Y G +++  Y+Q  Q  ++A ++ S  LA  YI    S++FARGHLSP+GDF+    QDA++Y++N APQWQ FN GNW  LE   R+    RG  L +Y+GTH I  LAD+NG++  ++L  + N    LP PKYYWKV+HD  +++  A IG+NNP+   IT  ++    +C  L      D      +I  G +F C V  +  +    P+LG L+LLV
Sbjct:    6 FMVLVTALVLLPESTSADCTFNLDTSSPANPPHLVDSFGKIIQPT--LQNNVRIITLTENQVVTVACEGNGNVLTATNMQINSGKCATA----GSSLLI---GTQNL--EYGSLGCKTVVKETIKETGTC---SVGGTHIEIGWQV-STRFINQMTICHVKSTANTLYSINT----ISG-ANIAADDKSNLRPSFRKGGFYVGIDVDAGYSQTGQNISVANIVGSAGLATTYINLSKSYFFARGHLSPDGDFIDAGSQDATYYYINAAPQWQSFNNGNWKSLEFGVRNLAISRGFDLVIYTGTHEILTLADINGIQKPIYLAKDSNNNNLLPAPKYYWKVVHDPVTNTATAVIGINNPHLTTITGADVFCADVCNQLTWMTWTDRF----EISKGYMFCCTVESLSNVVAHAPNLGNLNLLV 403          

BLAST of duplex-specific nuclease vs. nr
Match: gi|217272469|dbj|BAH02823.1| (duplex-specific nuclease [Paralithodes camtschaticus])

HSP 1 Score: 224.172 bits (570), Expect = 2.806e-65
Identity = 137/409 (33.50%), Postives = 207/409 (50.61%), Query Frame = 0

Query:   25 FAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNE-FATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ--LPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            F ++L+F SV G     DC+ + D  +P  PP+  DS  + I P   + +  R ++  +G  L L+C  S   N    E   A C GG   ++  +G E    +   LGC    K+        CG +D+G    IGF+      +  LI  C +     ++Y +H L+       +I  + RP +     ++   ++  Y+Q  Q + + ++L    LA+  I P    YFA+GH+SP+ DFV  + QDA++YF+N  PQWQ FN GNW YLE ATRD  E  G+ L VYSG   + QL D+NG  VD+ L     +  +PVP   WKV+++ +SS   A +G+NNP+IT        +C+ L S     D F  +D+  G  + C V+D+ +    IPDLG + LL 
Sbjct:   15 FLIVLLFVSVNG----QDCVWDKDTDFPEDPPLIFDSNLELIRPV--LENGKRIVSVPSGSSLTLACSGSELINLGM-EAVEAKCAGG--VMLAIEGTE---WEIWSLGCSNHVKETIRRNLGTCGEADQGDRHSIGFEYYGGSIYYELISVCFEPVSETTLYTEHVLHGANIAAKDIETS-RPSFKTSTGFFSVSMSTVYSQASQLQLMTDILGDSDLANNIIDPSQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHIT---TAPSPLCSDLCSSLTWID-FNLDDLAHGYTYCCAVDDLRQAIPYIPDLGNVGLLT 406          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1022763209|gb|KZS08846.1| (Uncharacterized protein APZ42_027051 [Daphnia magna])

HSP 1 Score: 223.787 bits (569), Expect = 4.054e-65
Identity = 136/424 (32.08%), Postives = 231/424 (54.48%), Query Frame = 0

Query:   14 NLQLLTMVKFCFAV-LLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQS---EFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNEFATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ---LPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            +L  L ++ F F   LL+ +S+     ++ C    D + P+ PP   D+ +  I P   +    R IT    +++ + C+ +   N+ Q+   + A A C+   + L+  DG E     +  LGC+ Q K+  ++ G+ C     G +I IG+Q    EF  L +TC     + + ++   ++ + +  ++ S+NERP + Q   + G ++N+ Y+Q  Q +TIA+++ S +LA QY+      + +RGH++P+GDF+  + QDA++YF+N APQWQ FNG NW  LE+A R+    R   L +Y+GT  I  LAD+NGV+  ++L  + N    +PVPKYYWK++HD ++++  A +G+NNP+I  D++    +  P   + +    +   DI  G  F C   ++ K     PDLG+L L V
Sbjct:    3 SLPWLILIAFAFQTNLLLVKSLPQF--KAGCTFNLDSSSPSNPPHLIDANNNIILP--EMVGSSRVITLNTDDKVAVGCLGT--GNYIQTTGMQLAPATCLSTSKLLL-VDGRE---LSYSQLGCLKQNKEILLENGT-CANG-LGTVIRIGWQAGP-EFIPLYDTCHDKVAAANYFSTSFIHGK-SAAADDSSNERPSFSQGGYFPGIDVNNAYSQSQQTQTIADIVGSTSLAGQYVVAGSQIFLSRGHMAPDGDFIDAASQDATYYFLNTAPQWQNFNGANWNVLENALRNLAISRDVKLTIYTGTFDILTLADINGVQQKIYLEFDENNNGLIPVPKYYWKLIHDPSTNTATAVLGINNPHI--DQVLPADVICPDVCNQVPWVTWDLTDIYKGYTFCCTSAELFKAINFAPDLGDLPLFV 410          

BLAST of duplex-specific nuclease vs. nr
Match: gi|26892281|gb|AAN86143.1| (duplex-specific nuclease [Paralithodes camtschaticus])

HSP 1 Score: 219.935 bits (559), Expect = 9.233e-64
Identity = 136/409 (33.25%), Postives = 205/409 (50.12%), Query Frame = 0

Query:   25 FAVLLVFRSVIGIASQSDCIIEPDKAWPAFPPIFQDSLSQFIWPTGSIYSQDRKITFVNGEQLELSCIKSTFENFPQSEFASAVCVGGDEFLVTADGEEDIVAKFQDLGCMLQPKDDAIDQGSLCGRSDEGLLIEIGFQMRDNE-FATLIETCLQVAESRSIYAKHTLYPEITGRSNISANERPYYFQPDEYYGYEINDFYTQVMQKETIAELLQSQALADQYIRPDDSFYFARGHLSPNGDFVYFSHQDASFYFMNVAPQWQVFNGGNWYYLESATRDFVEERGAPLDVYSGTHGICQLADVNGVKVDLWLRPEFNQ--LPVPKYYWKVLHDSASSSGVAFIGVNNPYITEDEIEALKICTPLESHPILDDIFYPNDIELGILFACQVNDVDKIFEEIPDLGELSLLV 430
            F ++L+F SV G     DC+ + D  +P  PP+  DS  + I P   + +  R ++  +G  L L+C  S   N    E   A C GG   ++  +G E    +   LGC    K+        CG +D+G    IGF+      +  LI  C       ++  +H L+       +I  + RP +     ++   ++  Y+Q  Q + + ++L    LA+  I P    YFA+GH+SP+ DFV  + QDA++YF+N  PQWQ FN GNW YLE ATRD  E  G+ L VYSG   + QL D+NG  VD+ L     +  +PVP   WKV+++ +SS   A +G+NNP+IT        +C+ L S     D F  +D+  G  + C V+D+ +    IPDLG + LL 
Sbjct:   15 FLIVLLFVSVNG----QDCVWDKDTDFPEDPPLIFDSNLELIRPV--LENGKRIVSVPSGSSLTLACSGSELINLGM-EAVEAKCAGG--VMLAIEGTE---WEIWSLGCSNHVKETIRRNLGTCGEADQGDRHSIGFEYYGGSIYYELISVCFGPVSETTLRTEHVLHGANIAAKDIETS-RPSFKTSTGFFSVSMSTVYSQASQLQLMTDILGDSDLANNIIDPSQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHIT---TAPSPLCSDLCSSLTWID-FNLDDLAHGYTYCCAVDDLRQAIPYIPDLGNVGLLT 406