hypothetical protein DAPPUDRAFT_329423, maker-scaffold168_size293125-snap-gene-1.72 (gene) Tigriopus kingsejongensis

Overview
Namehypothetical protein DAPPUDRAFT_329423
Unique Namemaker-scaffold168_size293125-snap-gene-1.72
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000009041 (supercontig:LSalAtl2s:LSalAtl2s566:581101:585276:1 gene:EMLSAG00000009041 transcript:EMLSAT00000009041 description:"augustus_masked-LSalAtl2s566-processed-gene-5.3")

HSP 1 Score: 501.901 bits (1291), Expect = 3.397e-172
Identity = 246/448 (54.91%), Postives = 322/448 (71.88%), Query Frame = 0
Query:  279 VGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCK--TSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAATATPSDETAE 724
            V  + G+   +KFDR+L D PCSGDGT+RKNADIWPKW+   S NLHG Q+RI KRGLELL VGG++VYSTCSLNPVEDEAV+  +L++  DTV +VD S+ VPGLKFKPGL++W +ADK+   YSK+E+VPE  H+L+RP MFP  P+  E   L + MR+LPH QDTGGFFVA L+K   CPWE++RT +    ++E+G          V  +P KK  R+ G+KEDPF+YF ++  E  F++IK++FD  D L  +MFLTRCK  T R  LY+TS+ +R I+  N+DRLK+IN GVKAFA+CENKGS C  RLAQEG+ MT+PFLGKR+V  + KDLE  L + D++ PP+I       + Q ++L TGS+ F+F  K+ +    L +ELVGWKGK T+RAYVPKN+R+HYLRLIG DTS++EKNKFE +RE A      +++ E
Sbjct:    3 VHDSSGAKKNLKFDRILADVPCSGDGTLRKNADIWPKWSPHASNNLHGXQFRILKRGLELLEVGGRLVYSTCSLNPVEDEAVLTHMLKETRDTVRIVDGSSLVPGLKFKPGLTSWKIADKNFNIYSKFEDVPESMHSLIRPFMFP--PKNVESLGLSKAMRVLPHLQDTGGFFVAVLEKVKICPWESERTMNVKAPSSEDGNEV------PVTQEPPKKKPRFFGYKEDPFMYFNEE--EPVFEDIKKYFDIKD-LDSKMFLTRCKDITKRNVLYYTSSALREIITKNEDRLKLINAGVKAFARCENKGSVCLFRLAQEGARMTLPFLGKRLVFVTNKDLEXXLFSEDVDKPPEITLFDEKTQNQFNKLDTGSVGFIF--KSTDDHEQLRIELVGWKGKSTIRAYVPKNERIHYLRLIGSDTSRFEKNKFEERREKANDLEKENDSKE 437          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000003034 (supercontig:LSalAtl2s:LSalAtl2s1723:22498:25427:-1 gene:EMLSAG00000003034 transcript:EMLSAT00000003034 description:"maker-LSalAtl2s1723-augustus-gene-0.10")

HSP 1 Score: 195.282 bits (495), Expect = 1.784e-53
Identity = 118/335 (35.22%), Postives = 180/335 (53.73%), Query Frame = 0
Query:   62 RENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWY-PERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNV-IVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWV 394
            R N KF ++Y  +     E D  E + A +  LP SFR T    Q+  + +L E++F   L R+                       WY P   AWQ+    +     +A  ++   ++ E + G++ RQE VSM+P   LD+QPHH+VLDMCA+PGSKT Q +E +  D      G ++AN+ +  R   L+    +  S N  +  + D   FP I +      S++++FDR++CD PCSGDGT+RKN ++W  W+ A     H +QY IA R +ELL  GG MVYS+C++NP+E+EAV+ R+LRD E  + +++    +  L +  G  TW+
Sbjct:   35 RTNEKF-EYYYKRLCPQEEWD--EALHAMRQPLPLSFRFTSSSPQSFLL-SLFENKFGSNLKRV----------------------PWYEPFGYAWQIQGIHRWNLDSDA--EIQKNILREWDLGHLYRQELVSMVPVHFLDIQPHHRVLDMCASPGSKTTQALEYLGND------GFLIANEFNKIRVTNLIGNLHKFSSSNAHLTLSEDGRYFPDIVMK----DSSILQFDRIVCDVPCSGDGTIRKNPNVWLNWHPARGNERHHLQYSIAARSIELLKPGGIMVYSSCAMNPIENEAVISRLLRDFEGDIFIINPPNAI-ALNYAQG--TWI 328          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000001858 (supercontig:LSalAtl2s:LSalAtl2s131:708019:713057:1 gene:EMLSAG00000001858 transcript:EMLSAT00000001858 description:"maker-LSalAtl2s131-augustus-gene-7.18")

HSP 1 Score: 98.9821 bits (245), Expect = 2.308e-21
Identity = 83/281 (29.54%), Postives = 118/281 (41.99%), Query Frame = 0
Query:  181 ETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELL----AVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQK 457
            E  +G+   Q   SM+  + L  Q   ++LDM +APG KT       H    M   GL+ AND +  RC  +V    RL   N +V  HD    P I  G          FDRVL DAPCSG G + K+  +    +  +      +Q  +    ++ L      GG +VYSTCS+ P E+E+V+   L+  +  V+LV       G+ F  G+  +                     T  R   + PS        L R  R  PH  +  GF+VA L+K
Sbjct:  354 EYLAGHYILQGASSMLSTMALAPQEKERILDMASAPGGKTT------HIAALMKNTGLLFANDPNKKRCKAIVGNLHRLGITNTVVCCHDGRKMPSIMKG----------FDRVLLDAPCSGSGVVSKDPTVKTSKDEKDIARCAHLQKELILSAIDCLDAKSKTGGYLVYSTCSILPDENESVIEYALK--KRNVKLVPT-----GIDF--GVEGF---------------------TKFRENRYHPS------MSLTR--RYYPHTHNMDGFYVAKLKK 580          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000010613 (supercontig:LSalAtl2s:LSalAtl2s705:297106:300420:1 gene:EMLSAG00000010613 transcript:EMLSAT00000010613 description:"augustus_masked-LSalAtl2s705-processed-gene-3.20")

HSP 1 Score: 56.9954 bits (136), Expect = 9.180e-9
Identity = 50/190 (26.32%), Postives = 86/190 (45.26%), Query Frame = 0
Query:  184 SGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKR--LQSPNVIVSNHDASI-----FPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILR 366
            +G+I  Q+  S +P   L+V+    V+D CAAPG+KT Q+   +  +      G V A + D  R  +L    +R  +    V V N D +      FP +              + ++ D  CS  G +   +D+     G     L   Q+++ +  L+   V  K+ YSTCS++ +E+E V+  I++
Sbjct:  147 NGSIILQDKASTLPIQALNVKEGSIVMDACAAPGNKTTQIASCVGLN------GGVYAIERDEERIQILDQTLERAGIHKSLVKVCNIDFTTLNPDDFPDV--------------EYIVLDPSCSSSGIL---SDVTI---GNRLSKLSAFQFKMLEHALKFPNV-SKISYSTCSVHKIENEEVIEAIVK 309          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000000620 (supercontig:LSalAtl2s:LSalAtl2s109:659327:660546:-1 gene:EMLSAG00000000620 transcript:EMLSAT00000000620 description:"augustus_masked-LSalAtl2s109-processed-gene-6.2")

HSP 1 Score: 55.0694 bits (131), Expect = 3.850e-8
Identity = 37/110 (33.64%), Postives = 55/110 (50.00%), Query Frame = 0
Query:  194 SMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGD 303
            S++P L L++Q  + VLD+C+ PG K      A+   +T++P  L V NDS  SR    ++  + + +  V  S  D  I      G+         FDRV+CD PCS D
Sbjct:  272 SLLPVLALNIQEGNTVLDLCSGPGGK------ALAISQTLLPSKL-VCNDSSRSR----LNTVQNVFNQYVGSSGLDLDIDFTCSDGEDFFVDYESSFDRVICDVPCSTD 370          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Match: EMLSAG00000007186 (supercontig:LSalAtl2s:LSalAtl2s404:330934:333537:1 gene:EMLSAG00000007186 transcript:EMLSAT00000007186 description:"augustus_masked-LSalAtl2s404-processed-gene-3.6")

HSP 1 Score: 52.373 bits (124), Expect = 3.370e-7
Identity = 51/180 (28.33%), Postives = 79/180 (43.89%), Query Frame = 0
Query:  209 VLDMCAAPGSKTVQLIEAIHADETMIP-EGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFK 387
            +LDMC++PG KT      +HA E +    G V+A D    +   +    ++    NV     D         G+    S    FDR+L DAPCS  G  R    I  K+    S     +Q R+    + LL  GG +VYSTC++   E+E     +L   +  +E+   +  + G+ +K
Sbjct:  242 MLDMCSSPGGKT------LHASEILQGLGGYVLAVDKSTKKIETIRKNLEKYGIENVSTLVGD---------GRTLCDSLHQTFDRILVDAPCSALG-QRACFRIQMKFKEMLS--YPKVQKRLLDSAVGLLRPGGLLVYSTCTITLGENEDNCSWLLNKYKGILEVDITNPMLDGVGYK 403          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|74872722|sp|Q9W4M9.1|NSUN2_DROME (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=NOP2/Sun domain family member 2 ortholog)

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 317/695 (45.61%), Postives = 458/695 (65.90%), Query Frame = 0
Query:   29 RRGQRRQRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAP--IVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHA--DETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKS-NTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAENGKR-----TIDSESDEVASK-----PRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKT-SRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLG-KRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKF 706
            R+ Q+R+   K+  R         + + Y++I R+NA F+ +Y+ QK+   + +  + + + + +LP +FRVTGF+ +A A+ ++IE+Q F E  R   +          AP  +  P CL WYP  +A+QL+LTRKDIRR E  ++LHNFLI ET +G ISRQE VSMIPP+VLDV+P  KVLDMCAAPGSKT QLIEA+HA  +E  IP G V+AND DN+RCY+LVHQAKRL SP ++V+NHD+S+FP +   + DGS A++KFD++LCD PCSGDGT+RKN DIW KWN A + NLHGIQYRI +RG E+L VGG++VYSTCSLNP+E+EAV+ RI++D +  +ELVD    VPGLK+KPG++ W LA K  +  ++++EEVPE  HT++RP MFP   +E  K  LE+C+R+LPH QD+GGFFVA L+K+    +E     + V+ N E  K+      +D++   +  K     P++K  R  G+KEDP+V+F ++  + ++  IKEF+   ++L Q   LTRC T  +KN+Y+ S  +R+++ +N++ +K+INTGVK F +CEN+ +    RLAQEG   +  F+G  R +   ++DL +ML   D   PP   +L    + +  EL  GS    + ++         +  VGW+G  ++RAYV K++ +H LRL+G D SK+E NK+
Sbjct:   13 RKRQKRENGPKRTDR---------QAQPYEEIKRDNAFFIKYYQLQKICATDEEWTQFLASIRDNLPTTFRVTGFKDEAKALLSIIETQLFTEYVRAVAELHQ------KAPEDVERPLCLPWYPNGLAYQLHLTRKDIRRSEPLYRLHNFLIVETTAGGISRQEAVSMIPPIVLDVRPTDKVLDMCAAPGSKTAQLIEALHAAPEEHKIPPGFVLANDVDNNRCYMLVHQAKRLNSPCLLVTNHDSSVFPNLVTTKPDGSKAILKFDKILCDVPCSGDGTLRKNPDIWLKWNLAQAYNLHGIQYRIVRRGAEMLEVGGRLVYSTCSLNPIENEAVLQRIIKDADGALELVDAGHLVPGLKYKPGMTDWKLATKEVDQIFTRFEEVPESLHTIIRPGMFPLPADEMAKIGLEKCLRVLPHLQDSGGFFVAVLEKRRQLSFEKNDVVELVKLN-ETAKQPAAEPQVDADGKPIEEKSVPWGPQRKKRRLHGYKEDPYVFFGEN--DPDYQAIKEFYQLDESLSQRCLLTRCVTEKKKNIYYCSEPIRDLVLNNENNIKIINTGVKTFVRCENRHTVHPFRLAQEGLQTSNAFMGASRRIQVEREDLVMMLNCTDPTQPPSTHELKKETQERCKELGVGSCILKYVDQR------FTLYTVGWRGTSSLRAYVQKDETIHILRLLGADLSKFETNKY 683          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|148887181|sp|Q1HFZ0.2|NSUN2_MOUSE (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=Myc-induced SUN domain-containing protein; Short=Misu; AltName: Full=NOL1/NOP2/Sun domain family member 2)

HSP 1 Score: 592.423 bits (1526), Expect = 0.000e+0
Identity = 326/753 (43.29%), Postives = 451/753 (59.89%), Query Frame = 0
Query:   25 MGGIRRGQRRQRTDKKKPRDPNDWSGHKENRA-----YQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSP-EEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRT--------------------------YDQVE---------KNAENGKRTIDSESDE----VASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK-NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSP--CALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLL-VELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEI--KREAAATATPSDETAEK 725
            MG   RG+R Q+  + +  +     G K  +A     Y +IV+EN  F  +Y+  K+   E +  + + + +  LPA+ R+TG++S A  + + +++++F EL  L  +  G  V  P         L+WYPE +AW  NL+RK +R+     K H FL+SETESGNISRQE VSMIPPL+L+V+PHHK+LDMCAAPGSKT QLIE +HAD ++  PEG V+AND DN RCYLLVHQAKRL SP ++V NHDAS  P++ V   DG   ++ +DR+LCD PCSGDGTMRKN D+W KW   NS  LHG+Q RIA RG E LA GG+MVYSTCSLNPVEDEAV+  +L   E  +EL DVSA +PGLK+ PG+S W +  +   +++ W EVP+  HT +RP MFPP+  E+ +  HLERC+RILPH Q+TGGFFVA L KKAP PW NKR                            DQ E          ++E    T ++ES+E    V   P  K  +  GFKEDPFV+  +D  +  F  I++F+    +  +   LTR    +K  LY  S  +RN+L +N +++KVINTG+K + +  N G    CA RLAQEG     PF+  RI+T S +D++ +L   +    P    LS    +Q+ +L+ GS+   ++  +A   T    + L GW+GK ++R +VPKN+R+HYLR++G +    +K +  I     AA+   P DE A++
Sbjct:    1 MGRRARGRRFQQPPQPEGEEDASDGGRKRGQAGWEGGYPEIVKENKLFEHYYQELKIVP-EGEWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKELEDL--EVDGQKVEVPQP-------LSWYPEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTV-DVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRPTMFPPTDLEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKAPMPW-NKRQPKVQNKSAEAREPRVSSHVAATEGNPSDQSELESQMITGAGDSETAHNTENTESNEKKDGVCGPPPSKKMKLFGFKEDPFVFIPED--DPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCR-NNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQEN----PFFRKLSSEAYSQVKDLAKGSVVLKYEPDSANPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNENAASPEQPGDEDAKQ 734          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|148887180|sp|Q08J23.2|NSUN2_HUMAN (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=Myc-induced SUN domain-containing protein; Short=Misu; AltName: Full=NOL1/NOP2/Sun domain family member 2; AltName: Full=Substrate of AIM1/Aurora kinase B; AltName: Full=tRNA (cytosine-5-)-methyltransferase; AltName: Full=tRNA methyltransferase 4 homolog; Short=hTrm4)

HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 318/742 (42.86%), Postives = 439/742 (59.16%), Query Frame = 0
Query:   48 WSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPP-SPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAEN-------------GKRTI---------------------------DSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK-NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSP--CALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAE-GATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEI--KREAAATATPSDETAEKPTEGKENSTEGKQNST 741
            W G      Y +IV+EN  F  +Y+  K+   E +  + + A +  LPA+ R+TG++S A  + + +++++F EL  L  +  G  V  P         L+WYPE +AW  NL+RK +R+     K H FL+SETESGNISRQE VSMIPPL+L+V+PHHK+LDMCAAPGSKT QLIE +HAD  +  PEG V+AND DN RCYLLVHQAKRL SP ++V NHDAS  P++ +   DG   ++ +DR+LCD PCSGDGTMRKN D+W KW   NS  LHG+Q RIA RG E LA GG+MVYSTCSLNP+EDEAV+  +L   E  +EL DVS  +PGLK+ PG++ W +  K   +++ W+ VP   HT +RP MFPP  PE+ +  HLERC+RILPH Q+TGGFFVA L KK+  PW NKR      K+AE              GK T                             S+ D V   P  K  +  GFKEDPFV+  +D  +  F  I++F+    +  +   LTR    +K  LY  S  +RN+L +N +++KVINTG+K + +  N G    CA RLAQEG     PF+  RI+T S +D++++L   +    P    LS    +Q  +L+ GSI   ++  +A   A    + L GW+GK ++R +VPKN+R+HYLR++G +    +K +  I     AA+T  P ++  E    G+ NS + ++ ++
Sbjct:   34 WEG-----GYPEIVKENKLFEHYYQELKIVP-EGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDL--EVDGQKVEVPQP-------LSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQI-DVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPW-NKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPED--DPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCR-NNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQEN----PFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANS 751          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|123892686|sp|Q28E61.1|NSUN2_XENTR (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=NOL1/NOP2/Sun domain family member 2)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 324/760 (42.63%), Postives = 445/760 (58.55%), Query Frame = 0
Query:   39 KKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQK-VADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEK-FHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKR---------------------------------TYDQVEKNAENGK------RTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK-NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSP--CALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLL-VELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAATATPSDETAEKPTEGK---ENSTEGKQNSTEGKENSTE 749
            ++K R+   W        Y +I++EN  F  +Y+  K V D E D  + + + +  LPA+ R+TG++S A  + + ++ ++F EL  +  +  G  +  P         L+WYPE +AW  NL+RK IR+     K H FL++ETESGNISRQE VSMIPP++L VQPHHK+LDMCAAPGSKT Q+IE +HAD  +  PEG V+AND DN RCYLLVHQAKRL SP ++V NHDAS  P++ V + +GS  V+ +DR+LCD PCSGDGT+RKN D+W KW   NS  LHG+Q RIA RG+E LA GG+MVYSTCSLNPVEDEAV+  +L   E ++EL DV++ +PGLK+ PG++ W +  K   +Y KWE++P   HT +RP MFPP  EE  K  +L RCMRILPH Q+TGGFFVA L KKAP PW NKR                                   D+   + ENG+      ++  S++D V   P  K  +  GFKEDPFV+  +D  +  FD I+ F+    +  ++  LTR +  +K  LY  S  +RN+L  N +++KVINTG+K   +  N G    CA RLAQEG     PF+  RIVT S +D++V+L   +    P ++  S   + Q +    GSI   ++    E  T    + L GW+GK ++R++VPKN+R+HYLR++G +  K +    E                +KP EGK   E   + K +    KE S E
Sbjct:   27 RRKAREQAAWEC-----GYPEIIKENKLFEHYYQELKIVPDGEWD--KFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDI--EVDGQKIEAPQP-------LSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLV-ENNGSREVLYYDRILCDVPCSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPPKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPW-NKRQPKLQRRPPVSACDASIAVAPELVKAVTENSAGMADEPAVDTENGETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKEDPFVFVSED--DPIFDPIQTFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCR-NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQEN----PFLSKFSKETQKQANNFDMGSIVLKYEPDPQEPETLQCPIVLCGWRGKTSIRSFVPKNERLHYLRMMGVEVFKEKAEVLE----------------KKPVEGKACDEEHIDEKMDIDGAKEESKE 745          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|82228491|sp|Q4V7N2.1|NSUN2_XENLA (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=NOL1/NOP2/Sun domain family member 2)

HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0
Identity = 306/701 (43.65%), Postives = 427/701 (60.91%), Query Frame = 0
Query:   38 DKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVA-DNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEK-FHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKR------------------------------TYDQVEKNAENGK------RTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK-NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSP--CALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLL--VELVGWKGKETVRAYVPKNDRVHYLRLI 694
            +++K R+   W G      Y +I++EN  F  +Y+  K+  D E D  + + A +  LPA+ R+TG++S A  + + ++ ++F EL  +  +  G  +  P         L+WYPE +AW  NL+RK IR+     K H FL+SETESGNISRQE VSMIPP++L+VQPHHK+LDMCAAPGSKT Q+IE +HAD  +  PEG V+AND DN RCYLLVHQAKRL SP ++V NHDAS  P++ + + +GS  V+ +DR+LCD PCSGDGTMRKN D+W KW   NS  LHG+Q RIA RG+E LA GG+MVYSTCSLNPVEDEAV+  +L   E ++EL DV++ +PGLK+ PG++ W +  K   ++ KWE+VP   HT +RP MFP   EE  K  +L RCMRILPH Q+TGGFFVA L KKAP PW NKR                                D+   +AENG+      ++  S++D V   P  K  +  GFKEDPFV+  +D  +  F+ I++F+    +  ++  LTR +  +K  LY  S  +RN+L  N +++KVINTG+K   +  N G    CA RLAQEG     PF+  RI+T S +D++V+L   +    P ++  S   + Q + L  GSI   + E + +   TL   + L GW+GK ++R++V    R++ ++ I
Sbjct:   22 ERRKAREQAAWEG-----GYPEIIKENKLFEHYYQELKIVPDGEWD--KFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDI--EVDGQKIEAPQP-------LSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPVLLNVQPHHKILDMCAAPGSKTAQIIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPRLLI-ENNGSREVLYYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLDKSEGSLELADVASELPGLKWMPGITQWRVMTKEGQWFEKWEDVPTSRHTQIRPTMFPLKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAPMPW-NKRQPKLQRRPPVSVCDASVAPEIVKAVADISAIADEPAVDAENGETKPCTNQSGSSKTDSVCCPPPSKKMKLFGFKEDPFVFLSED--DPIFEPIQKFYALDPSFPKKNLLTRTQEGKKRQLYMVSKELRNVLLHNSEKMKVINTGIKVLCR-NNDGEQYGCAYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQEN----PFLSKFSKETQKQANNLDMGSIVLKY-EPDPQQPETLQCPIVLCGWRGKTSIRSFVFFLHRINLVQWI 696          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|82082708|sp|Q5ZLV4.1|NSUN2_CHICK (RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase; AltName: Full=NOL1/NOP2/Sun domain family member 2)

HSP 1 Score: 556.599 bits (1433), Expect = 0.000e+0
Identity = 323/773 (41.79%), Postives = 452/773 (58.47%), Query Frame = 0
Query:   44 DPNDWSGHKENRAYQDIVRENAKFVDFYRAQK-VADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEK-FHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEK-------------NAENG------KRTI--DSE---------SDEVASK-------PRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK-NLYFTSNLVRNILESNQDRLKVINTGVKAFAK-CENKGSPCALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLL--VELVGWKGKETVRAYVPKNDRVHYLRLIGGDT--SKYEKNKFEIKREAAATATPSDETAEKPTEGKENSTEGKQNSTEGKENSTEGKEDSIEGKENSTEGKENSTE 770
            D   W+G      Y +IV+EN  F  +YR Q+ V D E D    + A +  LPA+ R+TG++S A  + + ++ ++F EL  L  +  G  V  P A       L+WYPE +AW  NL+RK +R+     + H FL+SETE GNISRQE VSMIPPL+L+V P HK+LDMCAAPGSKT QLIE +HAD  +  P+G V+AND DN RCYLLVHQAKRL SP ++V NHDAS  P + V   DG    + +DR+LCD PCSGDGTMRKN D+W KW   NS  LHG+Q RIA RG+E LA GG+MVYSTCSLNP+E+EAV+  +L   +  +EL DVS+ +PGLK  PG++ W +  K   ++ +W++VP    T +RP MFP   EE  K  +LERC+RILPH Q+TGGFFVA L KK+P PW NKR     +K             NA +G      K T+  D E         SD   SK       P KK+  + GFKEDPFV+  +D  +  F  I++F+    +  +   LTR +  +K  LY  S  +RN+L +N +++KVINTG+K +++  + +   CA RLAQEG     PF+  RIV    +D++++L   +    P ++  S   + ++ +++ GSI   + E + E    L   + L GW+GK ++RA+VPKN+R+HYLR++G +   +K ++ + E K E      P+        E KE     K  +   +E+     E S    EN +E  +  ++
Sbjct:   30 DQAGWAG-----GYPEIVKENELFERYYREQRIVPDGEWD--AFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHL--EVDGQKVEMPQA-------LSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDHKILDMCAAPGSKTAQLIEMLHADMNVPFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPNLQV-DVDGRKETLFYDRILCDVPCSGDGTMRKNIDVWKKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALELADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFPIKEEEKLKAMNLERCIRILPHHQNTGGFFVAVLIKKSPMPW-NKRQPKVHQKLPGKTEDTEVTATNAGDGSEDATEKPTLAEDEEPKKVQELQNSDTEQSKKGVCGPPPSKKMKLF-GFKEDPFVFLPED--DPLFLPIQKFYALDPSFPKMNLLTRTQEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWSRNSDGEQFGCAFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQEN----PFLSKFSSETQRKVKDMAMGSIVLKY-EPDPEKPDDLQCPIVLCGWQGKTSLRAFVPKNERLHYLRMMGVEVFKAKRKEGESEGKTEEEVQCRPAQTEEGMDVEDKERDAVTKMEAEIDEESPRSPVESSAMEIENKSEDSDQCSK 776          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|391358184|sp|Q9HGQ2.3|TRM4A_SCHPO (RecName: Full=Multisite-specific tRNA:(cytosine-C(5))-methyltransferase trm4a; AltName: Full=tRNA (cytosine-5-)-methyltransferase trm4a)

HSP 1 Score: 405.216 bits (1040), Expect = 1.030e-129
Identity = 224/596 (37.58%), Postives = 334/596 (56.04%), Query Frame = 0
Query:   60 IVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFF--LELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADE------TMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYD--------------QVEKNAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDA-LKQEMFLTRCKTS--RKNLYFTSNLVRNILESNQDRLKVINTGVKAFAK---------CENKGSPCALRLAQEGSLMTIPFLGKR-IVTPSKKDLEVML 620
            I R+N  F  +YR QK+   E D   + +     LP +FR+T     A  VR+     ++  +E AR           T  A I  P  L WYP+ MA+ L+++++ IR+      L  FL+ ETE+G+I+RQE+VSM+PPL+L+V+ HHKVLDMCAAPGSKT QL+EA+H         T++P G+V+ANDSDN R ++LVHQ KRL SPNV++ NHDAS  P  H+   DG    +KFDR+L D PCSGDGT RKN  +W +W+   +  LH  Q +I  RGL+LL  GG++VYSTCSLNP+E+EAVV  +L     +V LVDVS+ +P LK   G+  WV+ D     Y  ++ +P+  +  M P ++P   +E  + +++ C+RI PH Q+TGGFFVA L+K        K   D              Q  K  +   +   S+S     KP+ K    +  + DPFVY K+D  +   ++I + F   +A +K+  F  R       K +Y +++L RN++E+N++R+K ++ G+K F +            K   C  R+  +G+ +   F+ +  +   +  DL ++L
Sbjct:   24 IERKNENFEKYYRLQKLV-TEDDFILLKQKLTEQLPTTFRITASIPHATQVRDYFIEHYYPLIENAR-----------TEDAKIPLPVSLPWYPDGMAFMLDISKEVIRKSPHLKALQEFLVLETEAGDINRQESVSMVPPLLLNVESHHKVLDMCAAPGSKTAQLLEALHKPTKKEDITTLLPSGIVIANDSDNKRAHMLVHQIKRLNSPNVLIVNHDASFLPNFHLSSPDGKK-FLKFDRILADVPCSGDGTFRKNIALWNEWSLKTALGLHATQIKILMRGLQLLEKGGRLVYSTCSLNPIENEAVVSAVLNATRGSVRLVDVSSELPQLKRSQGVDNWVVCDSDLNIYPSFDTLPKELYEKMPPTLWPLPKKELAELNIQNCLRIYPHFQNTGGFFVAVLEKYENLTSSMKTAVDDNKVFLREQKLPSEQASKKRKQDTQETSSDSKLAEVKPKGKNGGNRFHELDPFVYIKED--DQALEKIYKKFGIDEAIIKKNQFFVRNVNGVPTKAIYISNDLFRNVIENNRNRVKFVHGGLKIFVRQDFGSLSREIAEKNGTCVFRVQSDGANLASHFIAESCLFHTTLSDLFILL 604          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|586408|sp|P38205.1|NCL1_YEAST (RecName: Full=Multisite-specific tRNA:(cytosine-C(5))-methyltransferase; AltName: Full=Multisite-specific tRNA:m5C-methyltransferase; AltName: Full=tRNA (cytosine-5-)-methyltransferase NCL1; AltName: Full=tRNA methyltransferase 4)

HSP 1 Score: 399.823 bits (1026), Expect = 1.159e-127
Identity = 253/692 (36.56%), Postives = 369/692 (53.32%), Query Frame = 0
Query:   52 KENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHV-GQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAENGKRTIDSESDEVASK-PRKKLSRYQGFK----------EDPFVYFKKDTVEAEFDEIK---EFFDFSDALKQEMFLTRCKTSR--KNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEG-SLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPV-----VKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYL-RLIGGDTSKYEKNKFEIKREAAATATPS 719
            +  + + ++V+EN K+  +Y+   +   E    E  +  Q  LP +FR+TG R  A  V NL + +    L  ++ +  G  +  P         L WYP+ +AWQL++ +  IR+ E + K   FL+ E   GNISRQE VSMIPP+VL+V+PHH VLDMCAAPGSKT QLIEA+H D T  P G VVAND+D  R ++LVHQ KRL S N++V NHDA  FP+I + G ++  + V+KFDR+LCD PCSGDGTMRKN ++W  WN      LH +Q  I  RGL LL   G++VYSTCSLNP+E+EAVV   LR   D + LV+    +PGL    G+S W + D++ T  +K +E      TL     F PS EEA KF+L+ CMR+ PHQQ+TGGFF+   +K              VE + E     + SE+  + S+ P+ K  + +  +          E+PFV+     V+ + + +K   +F+   +   +   L R  T    + +Y     +++++++N DRLK+I +GVK F   +     C+ R+  E   +M       RIV  + + L+ +L    IE+ P   D+           ++ +LS+G  AF+   +N      L + +  WKG + +   V K D    L R+ G D              A A ATPS
Sbjct:   22 RAQKNWSELVKENEKWEKYYKTLALFP-EDQWEEFKKTCQAPLPLTFRITGSRKHAGEVLNLFKERHLPNLTNVEFE--GEKIKAPVE-------LPWYPDHLAWQLDVPKTVIRKNEQFAKTQRFLVVENAVGNISRQEAVSMIPPIVLEVKPHHTVLDMCAAPGSKTAQLIEALHKD-TDEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHGNSNNKNDVLKFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGLGLHAVQLNILNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVSKWPVYDRNLTEKTKGDE-----GTL--DSFFSPSEEEASKFNLQNCMRVYPHQQNTGGFFITVFEK--------------VEDSTEAATEKLSSETPALESEGPQTKKIKVEEVQKKERLPRDANEEPFVF-----VDPQHEALKVCWDFYGIDNIFDRNTCLVRNATGEPTRVVYTVCPALKDVIQANDDRLKIIYSGVKLFVS-QRSDIECSWRIQSESLPIMKHHMKSNRIVEANLEMLKHLL----IESFPNFDDIRSKNIDNDFVEKMTKLSSGC-AFIDVSRNDPAKENLFLPV--WKGNKCINLMVCKEDTHELLYRIFGID--------------ANAKATPS 654          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|384872665|sp|O13935.2|TRM4B_SCHPO (RecName: Full=Multisite-specific tRNA:(cytosine-C(5))-methyltransferase trm4b; AltName: Full=tRNA (cytosine-5-)-methyltransferase trm4b)

HSP 1 Score: 398.282 bits (1022), Expect = 4.656e-127
Identity = 249/693 (35.93%), Postives = 370/693 (53.39%), Query Frame = 0
Query:   55 RAYQD---IVRENAKFVDFYRAQKVADNEAD--LAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-----------IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHV---GQADGSSAV-----MKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTW-VLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNA-------ENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTS--RKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCE------------NKGSPCALRLAQEGSLMTIPFLG-KRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATT-LLVELVGWKGKETVRAYVPKNDRVHY-LRLIGGDT 698
            RAY D   IV EN +F  +Y+ Q +   + +      +   +  LP +FR+ G+R  A  ++N  E  +   L  +  +  G T+  PT        L WYP+ +A+ ++  +  IR+     +L  FL+SE E+GNI+RQE VSM+PPL LDV+PHH +LDMCAAPGSKT QLIEA++    +             EGLV+AND+D  R  +LVHQ  RL SPN++V NHDAS  P I+V     +DG + +     +KFDR+L D PCSGDGT RKN  +W +W+  ++ +LH +Q RI  RGL+LL VGG +VYSTCS+NP+E+EAVV   L+     V LVDVS  +P LK  PGL +W VL D  N F S  E   +     +   M+P   EE  K H+ERC R+ PH Q+TGGFFVA LQK  P    N R++D  +  A       EN ++  +   DE ++    K S    F E+PFVY   D  +     I +F+    +  ++ F  R ++    +++YF  +L + I+E+N +R+K ++ GV+ F K E            NK   C  R+   G  +  PFL  K       KDL++ L+ N+     + ++ S ++K + +++  G        +  +GA   +L+    W+   +    + + ++ +  L L G D 
Sbjct:   13 RAYNDKSEIVLENKQFEGYYKKQNLFRGKPNDEFDSFMEYMRKPLPTTFRICGYRHHAFELKNHFEKYYVPSLKNVVHE--GQTIPPPTV-------LPWYPDGLAYIVDAQKDVIRKSPPLKRLQRFLVSENEAGNINRQEAVSMLPPLFLDVEPHHVILDMCAAPGSKTAQLIEAVYKKANIKDAAHDSKNLKSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVNHDASTMPNIYVKGSSPSDGLNVIEEKKILKFDRILADVPCSGDGTFRKNLSLWREWSANSAFSLHPLQLRILIRGLQLLKVGGCLVYSTCSINPIENEAVVTAALKATGGAVSLVDVSKKLPLLKRDPGLLSWKVLDDSLNEFQSPAENTND--KIELTESMWPLPEEEMSKLHIERCARLYPHMQNTGGFFVAVLQKTDPI---NSRSFDPKKYTASMEILPPENKRQRTEKGVDEASNSTLTK-SGNSYFDEEPFVYINPD--DTSIKTIVDFYGIDPSFPRDQFFVRNQSGIPVRSIYFACSLFKEIIEANTNRVKFVHGGVRFFVKQEISQLLKDFSLKANKDI-CNFRIHSNGVNIISPFLNEKHFYDAGLKDLKI-LVKNEYPHVEQFSE-SGMLKKEFEKMPLGCNILRVDAQTKDGALMDMLILQPIWRSPTSCNLMLARKEKQNLSLELFGMDV 685          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Match: gi|146325696|sp|Q3IHA0.2|RSMF_PSEHT (RecName: Full=Ribosomal RNA small subunit methyltransferase F; AltName: Full=16S rRNA m5C1407 methyltransferase; AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmF)

HSP 1 Score: 118.242 bits (295), Expect = 7.844e-27
Identity = 72/202 (35.64%), Postives = 107/202 (52.97%), Query Frame = 0
Query:  184 SGNISRQETVSMIPPLVLDVQPHHK--VLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPG 383
            SG +  QE  SM+PP+ L     H   VLDM +APGSKT QL   ++       +G++VAN+  +SR  +L    KR+   N  +S+ D  IF         G+     FD +L DAPCSG+GT+RK+AD    W+  ++  +  +Q  + K     L  GG +VYSTC+L P+E++ V   +L +  D +    +S   PG
Sbjct:   95 SGAMYVQEASSMLPPIALKQSIEHTNYVLDMASAPGSKTSQLAALMNN------QGVLVANELSSSRLKVLSATLKRMGVGNCALSHFDGVIF---------GNYMFECFDSILLDAPCSGEGTVRKDADALKNWSIDSNIEIAQVQKDLIKSAFYALKPGGTLVYSTCTLTPLENQHVCDFLLSEFGDCIIPESLSDLFPG 281          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|1325270134|ref|XP_023319648.1| (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 771.541 bits (1991), Expect = 0.000e+0
Identity = 375/735 (51.02%), Postives = 507/735 (68.98%), Query Frame = 0
Query:   25 MGGIRRGQRRQRTDKKKPRDPNDWSGHK---------ENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQA--DGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENK--RTYDQVEKNAENGKRTIDSESDEV--------------------------------ASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK---NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKRE 711
            MGGIRR  +  +  + +P    D SG +         +NR Y+DIVREN  FVD+YR Q +   E +   M+++ Q DLPASFR+TGFRSQ   +  ++E  +F E   L  ++ GG V  PT       CL WYP+RMA+QLN+TRKDIRR+E YF+LHNFL++ETE+GN SRQETVSMIPPLVLDV+PHHK+LDMCAAPGSKT QLIEA+H DE  +P G +VAND+DN+RCY+L HQAKRLQSP V+++NHDA+I P + V ++  D     +KFDR+LCD PC+GDGTMRKN DIWPKWN  N  NLHG+QYRI KRG+ELL VGG++VYSTCS NP+E+EAV+HR+L++ E ++EL+DV + +PGLK+ PGLS+W+LAD+    Y+  E+VPE   T++RP MFPP+PEEA K++L +C+RILPHQQ+TGGFFVA + KKA   WE K  + ++  +   +NG   ++   DE                                    P KK  RYQGF+EDPF Y +++  +  F  IK ++     L  E FL R K   K   NLYFT+  V++I+E+N++++K+INTGVKAF +CENKG+ C  R+AQEG++MT+ F+  R + PS+ D+E +L ++D + PP+  D+SP  + QL   STGS+A +F +K+ +G     VE++GWKGK T+RAYVPKN+RVHYLRL+G D S++EKNKF+ K++
Sbjct:    1 MGGIRRRGKNNKPRRDRPTPVYDESGQRIDDRNTEEGKNRGYKDIVRENVGFVDYYRTQNICPKE-EFDAMIQSLQQDLPASFRITGFRSQTKPLLKIMEGDYFQEFTEL--EKKGGKVVVPT-------CLEWYPDRMAYQLNVTRKDIRRDEIYFRLHNFLVAETETGNTSRQETVSMIPPLVLDVKPHHKILDMCAAPGSKTAQLIEALHQDEGGLPTGFLVANDADNARCYMLTHQAKRLQSPCVLITNHDAAIMPNMMVKKSPQDPELVPLKFDRILCDVPCTGDGTMRKNPDIWPKWNVMNGPNLHGLQYRILKRGIELLEVGGRLVYSTCSFNPIENEAVIHRLLKEGEGSLELLDVESFLPGLKYSPGLSSWILADRQGNSYTSMEDVPESLVTIIRPQMFPPAPEEAAKYNLNKCIRILPHQQNTGGFFVALIGKKALGGWEKKEKKNWEYKKGAGKNGNEDVNGAEDEGKNGKEEEGKEGDGKENEKEEEKGHNKHYREKDGGGPPKKKGRYQGFREDPFTYLQEN--DPLFATIKSYYGLE--LDSECFLRRSKDDSKKVCNLYFTTLQVKDIVENNKEKVKLINTGVKAFTRCENKGADCDFRIAQEGAVMTLNFIKDRRLFPSRPDMEKLLQSSDFDKPPENQDMSPEFRKQLGACSTGSVALIFIDKDKDGE-EYRVEIIGWKGKSTIRAYVPKNERVHYLRLVGVDVSRFEKNKFQDKKK 720          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|1325270132|ref|XP_023319647.1| (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 770 bits (1987), Expect = 0.000e+0
Identity = 375/738 (50.81%), Postives = 507/738 (68.70%), Query Frame = 0
Query:   25 MGGIRRGQRRQRTDKKKPRDPNDWSGHK---------ENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQA-----DGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENK--RTYDQVEKNAENGKRTIDSESDEV--------------------------------ASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTSRK---NLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKRE 711
            MGGIRR  +  +  + +P    D SG +         +NR Y+DIVREN  FVD+YR Q +   E +   M+++ Q DLPASFR+TGFRSQ   +  ++E  +F E   L  ++ GG V  PT       CL WYP+RMA+QLN+TRKDIRR+E YF+LHNFL++ETE+GN SRQETVSMIPPLVLDV+PHHK+LDMCAAPGSKT QLIEA+H DE  +P G +VAND+DN+RCY+L HQAKRLQSP V+++NHDA+I P + V ++     D     +KFDR+LCD PC+GDGTMRKN DIWPKWN  N  NLHG+QYRI KRG+ELL VGG++VYSTCS NP+E+EAV+HR+L++ E ++EL+DV + +PGLK+ PGLS+W+LAD+    Y+  E+VPE   T++RP MFPP+PEEA K++L +C+RILPHQQ+TGGFFVA + KKA   WE K  + ++  +   +NG   ++   DE                                    P KK  RYQGF+EDPF Y +++  +  F  IK ++     L  E FL R K   K   NLYFT+  V++I+E+N++++K+INTGVKAF +CENKG+ C  R+AQEG++MT+ F+  R + PS+ D+E +L ++D + PP+  D+SP  + QL   STGS+A +F +K+ +G     VE++GWKGK T+RAYVPKN+RVHYLRL+G D S++EKNKF+ K++
Sbjct:    1 MGGIRRRGKNNKPRRDRPTPVYDESGQRIDDRNTEEGKNRGYKDIVRENVGFVDYYRTQNICPKE-EFDAMIQSLQQDLPASFRITGFRSQTKPLLKIMEGDYFQEFTEL--EKKGGKVVVPT-------CLEWYPDRMAYQLNVTRKDIRRDEIYFRLHNFLVAETETGNTSRQETVSMIPPLVLDVKPHHKILDMCAAPGSKTAQLIEALHQDEGGLPTGFLVANDADNARCYMLTHQAKRLQSPCVLITNHDAAIMPNMMVKKSPQLSQDPELVPLKFDRILCDVPCTGDGTMRKNPDIWPKWNVMNGPNLHGLQYRILKRGIELLEVGGRLVYSTCSFNPIENEAVIHRLLKEGEGSLELLDVESFLPGLKYSPGLSSWILADRQGNSYTSMEDVPESLVTIIRPQMFPPAPEEAAKYNLNKCIRILPHQQNTGGFFVALIGKKALGGWEKKEKKNWEYKKGAGKNGNEDVNGAEDEGKNGKEEEGKEGDGKENEKEEEKGHNKHYREKDGGGPPKKKGRYQGFREDPFTYLQEN--DPLFATIKSYYGLE--LDSECFLRRSKDDSKKVCNLYFTTLQVKDIVENNKEKVKLINTGVKAFTRCENKGADCDFRIAQEGAVMTLNFIKDRRLFPSRPDMEKLLQSSDFDKPPENQDMSPEFRKQLGACSTGSVALIFIDKDKDGE-EYRVEIIGWKGKSTIRAYVPKNERVHYLRLVGVDVSRFEKNKFQDKKK 723          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|321458018|gb|EFX69093.1| (hypothetical protein DAPPUDRAFT_329423 [Daphnia pulex])

HSP 1 Score: 727.628 bits (1877), Expect = 0.000e+0
Identity = 353/692 (51.01%), Postives = 472/692 (68.21%), Query Frame = 0
Query:   25 MGGIRRGQRRQRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAENGKRTIDSES--DEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTS-RKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGKRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAA 713
            MG + R  +R++ D         W    +N  Y  +V+EN+ F  +Y+AQK+  ++      +   +  LPASFR++G R+Q  A+  ++E ++F     L E+Q+            +P+CLTWYPE +AWQL+LTRKDIR+ EA  KLHNFL+SETE+GNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKT QL+E +H DE   PEGLV+ANDS+N RCYLL HQ KRL SPN+I++NHDAS+ P  H+    G   V+KFDR+LCD PCSGDGT+RKN D+W KWN AN  +LHG+QYRIA+RG E+L+VGGKMVYSTCSLNP+E+EAV+HR+L + + ++EL +VS  +PGLK+ PGLS WV+  +  T Y+  EEVPE    ++R  +FPP PE+A+KFHLERC+RILPHQQ+TGGFFV+ L+K AP PWE +     V         + + ES  D     P +K  + +GFKEDP+++F +D  E  +  IKEF+  SD +  ++ LTRCK   +KN+Y+TS +VR++  +N+DRL +INTG+K FA+ ENKG+ C  R+AQEG+L  + F+ KR V  ++ D+ ++LL +D+ TPP+I   S     QL E+ TGS      E        L + +V WKGK +VRAYV K DR+HYLRL G D SK+E NKF  K  +A
Sbjct:    1 MGRMGRKNKREKKDGAAL----SWGTKNKNEGYDVVVKENSAFEMYYKAQKIVPDD-QWESFMYHLKEPLPASFRISGHRNQGKAILKIMEGEYF---KALTEEQA------------KPECLTWYPENLAWQLSLTRKDIRKSEANVKLHNFLVSETEAGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTSQLVEMLHCDEGKAPEGLVIANDSNNKRCYLLTHQLKRLPSPNLIITNHDASLMPNFHIPTPGGGKDVLKFDRILCDVPCSGDGTLRKNLDVWMKWNSANGSSLHGLQYRIARRGAEMLSVGGKMVYSTCSLNPMENEAVIHRLLVEAKGSLELEEVSGKLPGLKYVPGLSHWVVMSRVLTAYATPEEVPEAMKHILRGSLFPPKPEDADKFHLERCLRILPHQQNTGGFFVSALKKVAPLPWEAQERKPIVAATNSTAVVSTEVESTKDNAPRSPARKRQKIRGFKEDPYLFFGED--EELWPPIKEFYGLSDTMDPKLLLTRCKEGKKKNIYYTSPMVRDLTRTNEDRLTIINTGIKMFARSENKGTDCGYRIAQEGALAMMEFMKKRRVVITRDDMTMLLLNDDMNTPPEIHSFSSGAAKQLHEIDTGSTLMECYEDG------LHIVVVAWKGKTSVRAYVAKCDRIHYLRLCGADLSKFEVNKFAEKERSA 664          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|1070595297|ref|XP_018394790.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Cyphomyrmex costatus] >gi|1009380398|gb|KYN03352.1| tRNA (cytosine-5-)-methyltransferase CG6133 [Cyphomyrmex costatus])

HSP 1 Score: 721.079 bits (1860), Expect = 0.000e+0
Identity = 360/706 (50.99%), Postives = 479/706 (67.85%), Query Frame = 0
Query:   32 QRRQRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPW-----------------ENKRTYDQVEKNA-----ENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLG-KRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAA 713
            +RR++  K      N+W+   ++ +Y DI+REN  F  +Y+ Q +   E      +   +T+LP +FR+TG +++A A+  +I+S FF E+           V++ T  I+  + L +YPE +AWQL LTRKDIRR EAYF+LHNFLI+ET +G+ISRQE VSM+PPLVLDV+P HKVLDMCAAPGSKT QLIE IHADE   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD++I P   + ++DG+   +KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIAKRGLELLAVGG+MVYSTCSLNPVE+EAV+HR+L + ED+V+L+D    VPGL   PG++ W  A K+  +Y  WE+VPE   T +RP MFPP   EA KFHLERCMRILPH QDTGGFFVA L+K    PW                 ENK  +   E++A     E+GKRT++ E    + +P KK  +  G++EDPFV+F KD  E  +  IK+F+  SD L     L RC +  +KN+YFTS  +R+I+  N++++K+INTGVK F +C+NK   CA RLAQEG    I ++   R V  SK+DL ++L ++D  TPP+I  L+   + +L E +TGS   ++KE+  +    L ++LVGW+G  ++RAYVP  D +HYLRL+G D SK+EKNKFE  R AA
Sbjct:   16 KRREKQKK------NEWNSPSQSGSYADIIRENQDFESYYKTQGIVP-EDKWDAFISTIRTNLPVAFRITGSKAEAKALLEIIKSDFFKEILNTDMDSDKEDVNSQTKTIL--RSLPFYPEELAWQLQLTRKDIRRSEAYFRLHNFLIAETNNGSISRQEVVSMVPPLVLDVKPSHKVLDMCAAPGSKTAQLIEMIHADEENPPEGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSTIMPNFTITKSDGTKDTLKFDRILADVPCSGDGTMRKNPDIWCKWSPANGSNLHGIQYRIAKRGLELLAVGGRMVYSTCSLNPVENEAVLHRLLCETEDSVQLIDCRELVPGLICDPGVTHWKPASKNLQYYDSWEDVPEQWQTQVRPKMFPPKTNEASKFHLERCMRILPHHQDTGGFFVAVLEKTKSLPWERETCVLNQSTQNADGNENKDKHSLEEESAQDIISESGKRTLEEEKK--SREPPKKRKKMTGYREDPFVFF-KDQTEDVWQSIKDFYGISDDLDPRCLLVRCHEGKKKNIYFTSPAIRDIVICNENKVKMINTGVKTFVRCDNKNMNCAFRLAQEGMHSIIRYISDDRKVQISKEDLIMLLQSDDPHTPPEIIKLNSETQERLKEFATGSCILLYKEEKIDNPNRLNLQLVGWRGIMSLRAYVPTCDAIHYLRLLGADCSKFEKNKFEENRAAA 709          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|1069663873|ref|XP_018317077.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Trachymyrmex zeteki])

HSP 1 Score: 718.383 bits (1853), Expect = 0.000e+0
Identity = 363/718 (50.56%), Postives = 481/718 (66.99%), Query Frame = 0
Query:   39 KKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWE------NKRT----------------YDQVEKNAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLG-KRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAAT--ATPSDETAEKPTEGK 730
            +++ +  N+W+   ++ +Y DI+REN  F ++Y+ Q +   E      +   +T+LP +FR+TG +++A A+  +I+S FF E+           V+     I+    L +YPE +AWQL LTRKDIRR EAYF+LHNFLI+ET +G+ISRQE VSM+PPLVLDV+P HKVLDMCAAPGSKT QLIE IHADE   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD+SI P   + + DG+  ++KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIAKRGLELLAVGGKMVYSTCSLNP+E+EAV+HR+L + ED+V+L+D    VPGL   PG++ W  A K+  +Y+ WE+VPE   T +RP MFPP   EA KFHLE CMRILPH QDTGGFFVA L+K    PWE      N+ T                + +  K +E+GKRT++ E    + +P KK  R  G++EDPFV+F KD  E  +  IK+F+  SD L     L RC +  +KN+YFTS  +R+I+ SN++++K+INTGVK F +C+NK   CA RLAQEG    I ++   R V  SK+DL ++L  +D  TPP+I  L+   + +L E +TGS   ++KE+  +    L ++LVGW+G  ++RAYVP  D +HYLRL+G D SK+EKNKFE  R AA       SD   E  TE K
Sbjct:   17 RREKQKKNEWNNTSQSGSYADIIRENKDFENYYKTQGIVP-EDKWDAFISTIRTNLPVAFRITGSKAEAKALLEIIKSDFFKEILNADLDGDKEDVNGQAKTILHS--LPFYPEELAWQLQLTRKDIRRSEAYFRLHNFLIAETNNGSISRQEVVSMVPPLVLDVKPLHKVLDMCAAPGSKTAQLIEMIHADEENPPEGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSSIMPNFMITKPDGTKDILKFDRILADVPCSGDGTMRKNPDIWCKWSPANGSNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPIENEAVLHRLLCETEDSVQLIDCRDLVPGLICDPGVTHWKPASKNLQYYNSWEDVPEQWQTQVRPKMFPPDANEASKFHLEHCMRILPHHQDTGGFFVAVLKKVKSLPWERETCVLNQSTQNTDGNENKDKHILEEFAEDTKLSESGKRTLEKEKK--SREPSKKRRRMTGYREDPFVFF-KDQTEDVWQSIKDFYGISDDLDPRCLLVRCHEGKKKNIYFTSPAIRDIVISNENKVKMINTGVKTFVRCDNKNMNCAFRLAQEGMHSIIRYINDDRKVQISKEDLIMLLQNDDPHTPPEIVKLNSETQERLKEFATGSCILLYKEEKTDNPNRLNLQLVGWRGIMSLRAYVPTCDAIHYLRLLGADCSKFEKNKFEENRAAALAKDVNNSDVKTECETEVK 728          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|817085560|ref|XP_012265476.1| (tRNA (cytosine(34)-C(5))-methyltransferase isoform X1 [Athalia rosae] >gi|1183241769|ref|XP_020711173.1| tRNA (cytosine(34)-C(5))-methyltransferase isoform X1 [Athalia rosae])

HSP 1 Score: 717.998 bits (1852), Expect = 0.000e+0
Identity = 369/752 (49.07%), Postives = 507/752 (67.42%), Query Frame = 0
Query:   35 QRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVA-DNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMI-PEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWEN---KRTYDQVEKNAENGKR---TIDSESDEVAS------------------KPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGK-RIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEK---NAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAATATPSDETAEKPTEGKENSTEGKQNSTE--GKENSTEGKED 753
            +R D++K +D  +W   +  R Y++IVREN  F ++Y+ Q V  +NE ++   +   + +LPA+FR+TG +++  A+  +I+  FF E+   K + +   +       + PKCL +YP+ +AWQL LTRKDIRR EAY +LHNFLI+ET+SG+ISRQE VSMIPPLVLDV+PHHKVLDMCAAPGSKT QLIE IH+D++   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD+S+ P   V ++DG++  +KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIA+RGLELLAVGGKMVYSTCSLNP+E+EAV+HRIL +  D+V LVD    VPGLK  PG+S W  A K+  +Y  W+EVPE   T +RP MFPP+P + +KF LE+ MRILPH QDTGGFFVA L+K    PWEN   ++T + VE    N  R   +++ E+++ A+                   P++K  R  G++EDPFV+FK D  E  +  IKEF+D S+ L     L RC +  +KN+YFTS  +R+I++ N+ ++K+INTGVK F +C+NK   CA RLAQEG      ++G  R V  SK+DL  +LL +D +TPPKI+ LSP  ++++ + +TGS   ++KEK   +   A  L +++VGW+G  ++RAYVP +D +HYLRL+G D SK+EKNKF+  + A  +       A+    G E   E   N  E  G EN+ + ++D
Sbjct:   16 KRRDRQKNKD--EWD-KRPQRCYEEIVRENVDFENYYKTQNVIPENEWEV--FMDKMRENLPAAFRITGSKAETKALLEIIKGDFFNEIINTKLESNESEIKEEVEKKLYPKCLPFYPDSLAWQLQLTRKDIRRSEAYSRLHNFLIAETDSGSISRQEVVSMIPPLVLDVKPHHKVLDMCAAPGSKTAQLIEMIHSDDSNAHPEGFVIANDLDNNRCYMLVHQAKRLNSPNILITNHDSSVMPNFTVTKSDGTTGKLKFDRILADVPCSGDGTMRKNPDIWCKWSPANGNNLHGIQYRIAQRGLELLAVGGKMVYSTCSLNPIENEAVLHRILAEVGDSVRLVDGRDLVPGLKCNPGVSHWTPASKNMQYYKTWDEVPEQWQTQIRPHMFPPNPADVDKFCLEKSMRILPHHQDTGGFFVAVLEKVKLLPWENDQQEKTKEDVEGQTNNDNREELSLEDEAEKEAAGEKVADGKRPLELEKKPWGPQRKRKRIVGYREDPFVFFKNDK-EDVWPSIKEFYDISEMLDPTCLLVRCHEGKKKNIYFTSPAIRDIVKLNETKVKMINTGVKTFVRCDNKNMKCAFRLAQEGLHSLFHYVGDCRKVQISKEDLITLLLNDDPQTPPKISKLSPETQSRVKDFATGSCVLLYKEKPTVDENNANLLELQMVGWRGTMSLRAYVPLHDAIHYLRLLGADCSKFEKNKFKENQNAEKSK------AQVINGGTEQKMELTANVEESKGAENAVDMEQD 755          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|746860126|ref|XP_011060893.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Acromyrmex echinatior] >gi|332021238|gb|EGI61623.1| tRNA (cytosine-5-)-methyltransferase [Acromyrmex echinatior])

HSP 1 Score: 717.613 bits (1851), Expect = 0.000e+0
Identity = 360/704 (51.14%), Postives = 474/704 (67.33%), Query Frame = 0
Query:   32 QRRQRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPW-----------------ENKRTYDQVEKNAENGKRTIDSES---DEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGK-RIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAA 713
            +RR++  K      N+W+   ++ +Y DI+REN  F ++Y+ Q +   E      +   +T+LP +FR+TG +++A A+  +I+S FF E+           V+     I+    L +YPE +AWQL LTRKDIRR EAYF+LHNFLI+ET +G+ISRQE VSM+PPLVLDV+P HKVLDMCAAPGSKT QLIE IHADE   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD+SI P   + ++DG+   +KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIAKRGLELLAVGG+MVYSTCSLNP+E+EAV+HR+L + ED+V+L+D    VPGL   PG++ W  A K+  +Y  WE+VPE   T +RP MFPP   EA KFHLE CMRILPH QDTGGFFVA L+K    PW                 ENK  +   EK+A++ K + +SE    +E    P KK  R  G++EDPFV+F KD  E  +  IK+F+  SD L     L RC +  +KN+YFTS  +R+I+ SN++++K+INTGVK F +C+NK   CA RLAQEG    I ++   R V  SK+DL ++L  +D  TPP+I  L+   + +L E +TGS   ++KEK  +    L ++LVGW+G  ++RAYVP  D +HYLRL+G D SK+EKNKFE  R AA
Sbjct:   16 KRREKQKK------NEWNSASQSGSYADIIRENEDFENYYKTQGIVP-EDKWDAFISTIRTNLPVAFRITGSKAEAKALLEIIKSDFFKEILNADLDSDKEDVNNRAKTILHS--LPFYPEELAWQLQLTRKDIRRSEAYFRLHNFLIAETNNGSISRQEVVSMVPPLVLDVKPSHKVLDMCAAPGSKTAQLIEMIHADEENPPEGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSSIMPNFMITKSDGTKDTLKFDRILADVPCSGDGTMRKNPDIWCKWSPANGSNLHGIQYRIAKRGLELLAVGGRMVYSTCSLNPIENEAVLHRLLCETEDSVQLIDCRDLVPGLICDPGVTHWKPASKNLQYYDSWEDVPEQWQTQVRPKMFPPDANEASKFHLEHCMRILPHHQDTGGFFVAVLEKVKALPWEREICVLNQSTQNVDGNENKNKHILEEKSAQDTKLS-ESEKQTLEEEKKSPPKKRRRMTGYREDPFVFF-KDQTEDVWQSIKDFYGISDDLDPRCLLVRCHEGKKKNIYFTSPAIRDIVISNENKVKMINTGVKTFVRCDNKNMNCAFRLAQEGMHSIIRYISNDRKVQISKEDLIMLLQNDDPHTPPEIVKLNSETQERLKEFATGSCILLYKEKKTDNPNRLNLQLVGWRGIMSLRAYVPTCDAIHYLRLLGADCSKFEKNKFEENRAAA 708          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|1068416918|ref|XP_018053941.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Atta colombica] >gi|1009352891|gb|KYM78314.1| tRNA (cytosine-5-)-methyltransferase CG6133 [Atta colombica])

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 361/710 (50.85%), Postives = 475/710 (66.90%), Query Frame = 0
Query:   31 GQRRQRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWE--------NKRTYDQVE---------------KNAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLG-KRIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAAT 715
             ++R+   KK     N+W+   ++ +Y DI+REN  F ++Y+ Q +   E      +   +T+LP +FR+TG +++A A+  +I+S FF E+           V++    I+    L +YPE +AWQL LTRKDIRR EAYF+LHNFLI+ET +G+ISRQE VSM+PPLVLDV+P HKVLDMCAAPGSKT QLIE IHADE   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD+SI P   + + D +   +KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIAKRGLELLAVGG+MVYSTCSLNP+E+EAV+HR+L + ED+V+L+D    VPGL   PG++ W  A K+  +Y  WE+VPE   T +RP MFPP   EA KFHLERCMRILPH QDTGGFFVA L+K    PWE        N +  D  E               K +EN KRT++ E    + +P KK  R  G++EDPFV+F KD  E  +  IK+F+  SD L     L RC +  +KN+YFTS  +R+I+ SN++++K+INTGVK F +C+NK   CA RLAQEG    I ++   R V  SK+DL ++L  +D  TPP+I  L+   + +L E +TGS   ++KE+  +    L ++LVGW+G  ++RAYVP  D +HYLRL+G D SK+EKNKFE  R AA T
Sbjct:   14 AEKRREKQKK-----NEWNSASQSGSYADIIRENEDFENYYKTQGIVP-EDKWDAFISTIRTNLPVAFRITGSKAEAKALLKIIKSDFFKEILNADLDGDKEDVNSRAKTILH--SLPFYPEELAWQLQLTRKDIRRSEAYFRLHNFLIAETNNGSISRQEVVSMVPPLVLDVKPSHKVLDMCAAPGSKTAQLIEMIHADEENPPEGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSSIMPNFMITKPDDTKDTLKFDRILADVPCSGDGTMRKNPDIWCKWSPANGSNLHGIQYRIAKRGLELLAVGGRMVYSTCSLNPIENEAVLHRLLCETEDSVQLIDCRDLVPGLICDPGVTHWKPASKNLQYYDSWEDVPEQWQTQVRPKMFPPDANEASKFHLERCMRILPHHQDTGGFFVAVLEKVKSLPWEREICVLNQNTQNVDGNENKDKHILEEESAQDIKLSENEKRTLEEEKK--SREPPKKRRRMTGYREDPFVFF-KDQTEDVWQSIKDFYGISDDLDPRCLLVRCHEGKKKNIYFTSPAIRDIVISNENKVKMINTGVKTFVRCDNKNMNCAFRLAQEGMHSIIRYISDDRKVQISKEDLIMLLQNDDPHTPPEIVKLNSETQERLKEFATGSCILLYKEEKTDNPNRLNLQLVGWRGIMSLRAYVPTCDAIHYLRLLGADCSKFEKNKFEENRAAALT 712          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|998517101|ref|XP_015519362.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Neodiprion lecontei])

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 377/751 (50.20%), Postives = 503/751 (66.98%), Query Frame = 0
Query:   35 QRTDKKKPRDPNDWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVTGFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPTAPIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADETM-IPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQV--EK-----------------------NAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRC-KTSRKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGK-RIVTPSKKDLEVMLLAND-IETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAE---GATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAATATPSD--ETAEK---PTEGKENSTEGKQNSTEGKENST 748
            +R DK+K +D  +W   +  R Y++I+REN  F ++Y+ Q +  +E +    +   + +LPA+FR+TG +++  A+ ++I+  FF E+  +K +     V       + PKCL +YP+ +AWQL LTRKDIRR EAYF+LHNFLI+ET SGNISRQE VSMIPPLVL+V+P HKVLDMCAAPGSKT QLIE IH+DE+   PEG V+AND DN+RCY+LVHQAKRL SPN++++NHD+S+ P  +V + DG++  +KFDR+L D PCSGDGTMRKN DIW KW+ AN  NLHGIQYRIA+RGLELLAVGG+MVYSTCSLNP+E+EAV+HRIL +  D+V LVD    VPGLK  PG+S W LA K+  +Y  WEEVPE   T +RP MFPP+  + +KFHLER MRILPH QDTGGFFVA L+K  P PWENK    +V  EK                        A +GKR +++E       P++K  R  G++EDPFV+FK D  E  +  IKEF++ SD L     L RC +  +KN+YFTS  +R+I++ N+ ++K+INTGVK F +C+NK   CA RLAQEG      ++G+ R V  SK DL VMLL ND   TPP+I+ LSP  ++++ + +TGS   ++KE+  E       L +E+VGW+G  ++RAYVP +D +HYLRL+G D SK+EKNKF+  + A  +    D  E+ EK      GKE   E  +N  E   N++
Sbjct:   16 KRRDKQKNKD--EWD-KRPQRCYEEIIRENVDFENYYKTQNIVPSE-EWYIFMNKMRENLPAAFRITGSKAETKALLDIIKGDFFNEIINIKRENEEEEVKEKEK--LYPKCLPFYPDSLAWQLQLTRKDIRRSEAYFRLHNFLIAETASGNISRQEVVSMIPPLVLEVKPQHKVLDMCAAPGSKTAQLIEMIHSDESNPHPEGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSSVMPNFNVAKPDGTTEKLKFDRILADVPCSGDGTMRKNPDIWCKWSPANGNNLHGIQYRIAQRGLELLAVGGRMVYSTCSLNPIENEAVIHRILTEVGDSVRLVDGRNLVPGLKCNPGVSHWTLASKNLQYYKTWEEVPEQWQTQIRPHMFPPNAADVDKFHLERSMRILPHHQDTGGFFVAVLEKVKPLPWENKEEKMEVGSEKPSTNDANTELSLEEEAEKEEANLKATDGKRPLEAEKKPWG--PQRKRKRMVGYREDPFVFFKGDK-EDVWPSIKEFYNISDKLDPTCLLVRCHEGKKKNIYFTSPAIRDIVKLNEAKVKMINTGVKTFVRCDNKNMKCAFRLAQEGLNSLFDYIGECRKVRISKDDL-VMLLQNDNPNTPPEISKLSPDTQSRVQDFATGSCVLIYKEELIEEDKNLNPLELEMVGWRGTMSLRAYVPTHDAIHYLRLLGADCSKFEKNKFKENQNAETSNVELDDGESMEKGNTTANGKE--LEDNKNEEESSNNTS 754          
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Match: gi|970881700|ref|XP_015118498.1| (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Diachasma alloeum])

HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0
Identity = 369/721 (51.18%), Postives = 487/721 (67.55%), Query Frame = 0
Query:   25 MG-GIRRGQRRQRTDKKKPRDPN--DWSGHKENRAYQDIVRENAKFVDFYRAQKVADNEADLAEMVRAFQTDLPASFRVT-GFRSQAVAVRNLIESQFFLELARLKEQQSGGTVSTPT-APIVEPKCLTWYPERMAWQLNLTRKDIRREEAYFKLHNFLISETESGNISRQETVSMIPPLVLDVQPHHKVLDMCAAPGSKTVQLIEAIHADE-TMIPEGLVVANDSDNSRCYLLVHQAKRLQSPNVIVSNHDASIFPKIHVGQADGSSAVMKFDRVLCDAPCSGDGTMRKNADIWPKWNGANSCNLHGIQYRIAKRGLELLAVGGKMVYSTCSLNPVEDEAVVHRILRDCEDTVELVDVSAHVPGLKFKPGLSTWVLADKSNTFYSKWEEVPEVHHTLMRPPMFPPSPEEAEKFHLERCMRILPHQQDTGGFFVACLQKKAPCPWENKRTYDQVEKNAENGKRTIDSESDEVASKPRKKLSRYQGFKEDPFVYFKKDTVEAEFDEIKEFFDFSDALKQEMFLTRCKTS-RKNLYFTSNLVRNILESNQDRLKVINTGVKAFAKCENKGSPCALRLAQEGSLMTIPFLGK-RIVTPSKKDLEVMLLANDIETPPKIADLSPVVKAQLDELSTGSIAFVFKEKNAEGATTLLVELVGWKGKETVRAYVPKNDRVHYLRLIGGDTSKYEKNKFEIKREAAATATPSDETAEKPTEGKENSTEGK 737
            MG G RRG ++   DK++ R  N  +W   +  R Y+D+VR+N  F  FY+AQ++   E    E +   + +LP +FR+T G R++  A+  +I+  FF E+   K        STP   P V+P  L +YPE +AWQL LTRKDIRR EAYF+LHNFLI+ET SG+ISRQE VSMIPPLVLDV+P HKVLDMCAAPGSKT QLIE IHADE    P+G V+AND DN+RCY+LVHQAKRL SPN++++NHD+S+ P   +   DGS  ++KFDR+L D PC+GDGTMRKN DIW KWN AN  NLHGIQ+RIAKRGLE+LAVGG+MVYSTCSLNP+E+EAV+HR+L +  D+V LVD    VPGL   PG+S WV   K   FY  +EEVPE   TL+RP MFPP PEEA +FHLE+CMRILPH QDTGGFFVA L+K    PWE +R         + G+  +    ++  + P +K  R  G++EDPFV+FK +  E  +  IKEF+  SD L     L RC+   +KN+YFTS  +R+I+  N++ +K+INTGVK F +C+NK   CA RLAQEG    + ++G+ R V   ++DL  +L  ++ +TPP+IA LS  ++     + +GS   +++E +AE    L + +VGW+G  ++RAYVP +D VHYLRL+G D SK+EKNKFE  R A  T    D+T+  P+  +EN T+ K
Sbjct:    1 MGRGRRRGAKKNFGDKRRERQKNKDEWD-KRPQRHYEDMVRDNKDFETFYKAQRIVP-EDQWPEFLSKMKENLPVAFRITAGSRAETKALSKIIQGDFFTEILNAKLD------STPDDEPKVKPHRLPFYPEGLAWQLQLTRKDIRRSEAYFRLHNFLIAETSSGSISRQEVVSMIPPLVLDVKPDHKVLDMCAAPGSKTAQLIEMIHADEGNPHPKGFVIANDVDNNRCYMLVHQAKRLNSPNILITNHDSSVMPNFRMTSPDGSEGILKFDRILADVPCTGDGTMRKNPDIWCKWNPANGHNLHGIQFRIAKRGLEMLAVGGRMVYSTCSLNPLENEAVLHRLLCETGDSVRLVDGREFVPGLVCNPGVSHWVACSKDMKFYETFEEVPEQWQTLVRPKMFPPKPEEAGRFHLEKCMRILPHHQDTGGFFVAVLEKVKALPWEREREGSP----QDGGEGGVKRSGEQDKAGPERKKKRMFGYREDPFVFFKDE--EEVWPAIKEFYSISDDLDPRCLLVRCQEGKKKNIYFTSPAIRDIVLLNENNVKMINTGVKTFVRCDNKNMKCAFRLAQEGMPSILSYIGENRKVKIGREDLVELLQNDNPQTPPEIAKLSEEIQKGTVNIDSGSCILIYEESDAENLDPLKLVMVGWRGTMSLRAYVPLHDAVHYLRLLGADFSKFEKNKFEENRNANKTTEGEDDTSIFPS-NEENLTDEK 706          
The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_329423 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 6
Match NameE-valueIdentityDescription
EMLSAG000000090413.397e-17254.91supercontig:LSalAtl2s:LSalAtl2s566:581101:585276:1... [more]
EMLSAG000000030341.784e-5335.22supercontig:LSalAtl2s:LSalAtl2s1723:22498:25427:-1... [more]
EMLSAG000000018582.308e-2129.54supercontig:LSalAtl2s:LSalAtl2s131:708019:713057:1... [more]
EMLSAG000000106139.180e-926.32supercontig:LSalAtl2s:LSalAtl2s705:297106:300420:1... [more]
EMLSAG000000006203.850e-833.64supercontig:LSalAtl2s:LSalAtl2s109:659327:660546:-... [more]
EMLSAG000000071863.370e-728.33supercontig:LSalAtl2s:LSalAtl2s404:330934:333537:1... [more]
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BLAST of hypothetical protein DAPPUDRAFT_329423 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|74872722|sp|Q9W4M9.1|NSUN2_DROME0.000e+045.61RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|148887181|sp|Q1HFZ0.2|NSUN2_MOUSE0.000e+043.29RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|148887180|sp|Q08J23.2|NSUN2_HUMAN0.000e+042.86RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|123892686|sp|Q28E61.1|NSUN2_XENTR0.000e+042.63RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|82228491|sp|Q4V7N2.1|NSUN2_XENLA0.000e+043.65RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|82082708|sp|Q5ZLV4.1|NSUN2_CHICK0.000e+041.79RecName: Full=tRNA (cytosine(34)-C(5))-methyltrans... [more]
gi|391358184|sp|Q9HGQ2.3|TRM4A_SCHPO1.030e-12937.58RecName: Full=Multisite-specific tRNA:(cytosine-C(... [more]
gi|586408|sp|P38205.1|NCL1_YEAST1.159e-12736.56RecName: Full=Multisite-specific tRNA:(cytosine-C(... [more]
gi|384872665|sp|O13935.2|TRM4B_SCHPO4.656e-12735.93RecName: Full=Multisite-specific tRNA:(cytosine-C(... [more]
gi|146325696|sp|Q3IHA0.2|RSMF_PSEHT7.844e-2735.64RecName: Full=Ribosomal RNA small subunit methyltr... [more]

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BLAST of hypothetical protein DAPPUDRAFT_329423 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1325270134|ref|XP_023319648.1|0.000e+051.02tRNA (cytosine(34)-C(5))-methyltransferase-like is... [more]
gi|1325270132|ref|XP_023319647.1|0.000e+050.81tRNA (cytosine(34)-C(5))-methyltransferase-like is... [more]
gi|321458018|gb|EFX69093.1|0.000e+051.01hypothetical protein DAPPUDRAFT_329423 [Daphnia pu... [more]
gi|1070595297|ref|XP_018394790.1|0.000e+050.99PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]
gi|1069663873|ref|XP_018317077.1|0.000e+050.56PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]
gi|817085560|ref|XP_012265476.1|0.000e+049.07tRNA (cytosine(34)-C(5))-methyltransferase isoform... [more]
gi|746860126|ref|XP_011060893.1|0.000e+051.14PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]
gi|1068416918|ref|XP_018053941.1|0.000e+050.85PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]
gi|998517101|ref|XP_015519362.1|0.000e+050.20PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]
gi|970881700|ref|XP_015118498.1|0.000e+051.18PREDICTED: tRNA (cytosine(34)-C(5))-methyltransfer... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold168_size293125supercontigscaffold168_size293125:207567..210529 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
NoteP:methylation
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
maker-scaffold168_size293125-snap-gene-1.72-mRNA-1maker-scaffold168_size293125-snap-gene-1.72-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold168_size293125:207567..210529+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>maker-scaffold168_size293125-snap-gene-1.72 ID=maker-scaffold168_size293125-snap-gene-1.72|Name=hypothetical protein DAPPUDRAFT_329423|organism=Tigriopus kingsejongensis|type=gene|length=2963bp|location=Sequence derived from alignment at scaffold168_size293125:207567..210529+ (Tigriopus kingsejongensis)
CTTCCACAACACAAACACGTGAATTGGGACCGTGGGTTGGACTGGGTTGG ACCAAGTAGAGGCACTAGAGAAGGGACCAAGGACCTCTGTATCTAGAATT CCTGGGTTGGACTCTGCGATTTTCCAGTGTTCCAGGCTTGGGGGACTCTC CGTGGATCATGGGCGGAATCCGACGTGGGCAGCGTCGCCAGCGCACCGAT AAAAAGAAGCCTCGCGATCCGAATGACTGGTCGGGTCACAAGGAGAACCG GGCTTATCAAGATATCGTGCGCGAAAATGCCAAGTTTGTCGACTTCTACC GGGCCCAGAAGGTCGCTGACAACGAGGCCGACTTGGCCGAGATGGTACGC GCCTTTCAAACCGACTTGCCCGCCTCATTTCGCGTCACTGGCTTTCGCTC ACAGGTGAGCCCCGGAGGCCGGATCCGGATTGGAGGAATCCATCTCACTC TCGCTCTTTCCCTGAATGCGTCGACTAGGCCGTGGCCGTGCGCAATCTCA TCGAGAGCCAATTCTTCCTGGAATTGGCGCGCCTCAAGGAGCAGCAGAGT GGCGGCACGGTGAGCACACCCACGGCACCCATCGTGGAGCCCAAGTGTCT GACCTGGTATCCCGAGCGCATGGCGTGGCAACTCAATCTCACGCGCAAGG ATATCCGCCGCGAGGAGGCCTACTTCAAGCTCCACAACTTTCTCATCTCC GAGACCGAATCAGGTGAGTCCCAATCCCCACCATCCTCGAGACCTGGCTA GACCTGGCATGATTTCCTCTCAGGCAACATCAGTCGACAAGAGACCGTGT CCATGATTCCCCCCCTGGTGCTCGATGTGCAGCCACACCACAAGGTGCTG GACATGTGCGCCGCTCCCGGCTCCAAGACGGTCCAGCTCATCGAAGCCAT TCACGCGGATGAAACCATGATCCCCGAGGGTCTGGTCGTGGCCAACGATT CGGACAACAGCCGCTGCTATCTGCTCGTCCATCAGGCCAAGCGGCTCCAA AGTCCCAACGTGATTGTGAGCAACCACGACGCCTCCATCTTCCCCAAGAT TCACGTGGGCCAGGCCGATGGAAGCTCGGCCGTGATGAAGTTCGATCGCG TTCTCTGTGATGCGCCTTGCTCCGGCGACGGGACCATGCGCAAGAATGCT GATATCTGGCCGGTAAGCGTCCCGCTCCGTGGTTGGCCGCCGCTGGTTCT AATCCGAGGTGGTTATTTGCTTCTAGAAATGGAACGGGGCCAACAGTTGC AATCTGCACGGCATCCAGTATCGGATTGCCAAGCGCGGTCTGGAATTATT GGCCGTGGGTGGCAAGATGGTCTACTCCACGTGCTCGCTCAATCCGGTCG AAGATGAGGCGGTCGTGCATAGGTAAATGATGCCCTCAAATCAGAGCCTC CACCAGCCTTGACTAAATTAATTGCTTGCTTTCCTAGAATCCTCCGTGAT TGTGAGGATACGGTCGAGCTAGTCGACGTCTCGGCTCATGTACCGGGACT CAAGTTCAAGCCCGGCTTGTCAACCTGGGTCTTGGCCGACAAGAGCAACA CCTTCTACTCCAAGTGGGAAGAGGTGCCAGAAGTGCATCACACGCTGATG AGACCGCCCATGTTCCCTCCTAGTCCCGAGGAGGCCGAGAAGTTTCATCT CGAGAGGTAGTCGCCACTCATTTCGTCCATCGACTCGGATGATAAACCCC TTTTTTTCGTCTCCAGGTGTATGCGCATCCTGCCTCACCAACAAGATACG GGGGGATTTTTCGTGGCTTGCCTACAGAAGAAAGCCCCGTGCCCGTGGGA GAACAAGAGGACCTACGACCAAGTCGAAAAGAACGGTAACCAACCTCACT TTGCCCGATGTATGCATCGTAGTTAACCCCTCATTTATTCCGCTCAGCAG AAAATGGAAAGCGAACCATCGACTCTGAAAGTGACGAGGTCGCGAGCAAG CCGAGGAAGAAGCTGTCCCGATACCAGGGATTCAAAGAAGATCCCTTTGT CTACTTCAAGAAGGACACCGTGGAGGCTGAGTTTGACGAAATCAAGGAGT TCTTCGACTTTTCCGACGCCCTCAAGCAAGAGATGTTCCTCACTCGTTGC AAGACTAGCCGGAAGAATCTCTACTTCACCTCCAACCTCGTGCGAAACAT CCTGGAGTCCAATCAAGACCGCTTGAAAGTGATCAACACAGGGGTGAAAG CATTTGCCAAATGCGAAAACAAAGGCTCCCCCTGTGCCCTAAGGTGAGTT CAATCCCTCACTAGCTCACTGCTCATGGAGTATAAAGGATGATTCGTGTT TTAGACTCGCCCAAGAAGGCTCCTTGATGACCATTCCATTCCTTGGCAAG CGCATCGTGACGCCCTCCAAAAAGGATCTGGAGGTCATGCTGCTGGCCAA TGACATCGAAACACCTCCCAAAATTGCCGATCTCAGCCCAGTTGTAAAAG CTCAGCTAGACGAGTTGAGCACCGGAAGTATTGCCTTCGTCTTCAAGGAA AAGAACGCAGAGGGTAAGTGTGAATTGTCCGCCGAACGAACCATCATCTT GCACCTCCTGCATTGTTGCTCATGCATTTCCAGGAGCCACCACACTCCTG GTTGAGTTAGTGGGCTGGAAGGGCAAGGAGACCGTGCGCGCTTACGTCCC CAAGAATGATCGCGTCCACTACTTACGCCTCATCGGCGGAGACACCTCCA AGTACGAGAAGAACAAGTTTGAAATCAAACGTGAGGCGGCCGCCACCGCG ACTCCCTCGGACGAGACTGCCGAGAAACCGACCGAGGGGAAGGAGAACTC AACCGAAGGGAAGCAGAACTCGACCGAAGGGAAGGAGAACTCGACCGAAG GGAAGGAGGACTCGATCGAAGGGAAGGAGAACTCGACCGAAGGGAAGGAG AACTCGACCGAAGGGAAGGAGAACACGCCTATGGAAGCCACTGTGGATCC AGCCGGAGAATGA
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Synonyms
The feature 'hypothetical protein DAPPUDRAFT_329423' has the following synonyms
Synonym
Tk04473
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