BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000000690 (supercontig:LSalAtl2s:LSalAtl2s110:165584:172366:-1 gene:EMLSAG00000000690 transcript:EMLSAT00000000690 description:"augustus_masked-LSalAtl2s110-processed-gene-1.0")

HSP 1 Score: 746.503 bits (1926), Expect = 0.000e+0
Identity = 359/587 (61.16%), Postives = 433/587 (73.76%), Query Frame = 0

Query:    8 DKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDS--------SIREGAIK-------------------------------LDRELVNSSIDEVPDSV-KNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNESGNVLRD-SSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            D  WTF DLE YSNRVA Y I Q + PGDCVAL+M N PEY+G+WLGC+K G+VPALIN ++VG PL HSI   SA A I+G +L+  +  I   +PN++ +  GS +        S+  G+                                  D E+   S + VP  +    ++F  KLLY++TSGTTGLPKAAVIK SRFY++ AG YYLN+MG+I+D   Y+PLPLYHSAGG+VG+G+MMVFG  + IR+KFSVRNFWKDC +YNC GAQYIGEICRYLLS+PETPE+K H ++++FGNGLRP IW QFT RFG+ RI EFYG+TEGN+N++NID  PGAVGF+S LFP VYPVRL+R+ E GNVLRD  +GLC+LC+PGE GEFVGKI+KNH  RSFDGY+D  ATEKKIVRNVL +GD WFRSGDLL MDE GWMYF+DR+GDTFRWRGENVST EVEAVLSNVL Q+D IVYGVE+PG EGRAGMA IV     LD   FL  +K  LPAYA P+F+RLV+ LDITGTFKLKK  LQ EGY+P+VI DPL+F D+VKG YR LD  LYN+IM+M+ R+
Sbjct:   55 DVVWTFLDLESYSNRVARYLISQGFVPGDCVALYMENRPEYVGIWLGCAKAGIVPALINTHIVGNPLRHSIXVASAXAVIYGVELSKPINEIKSLLPNIKYYESGSTTTSPEYSEISVHHGSTSDHHDQQHSAQQLSFTDDKLLHNPNCDLTSKYFDTEIKKFSTESVPPPILDKSSNFRSKLLYVFTSGTTGLPKAAVIKQSRFYYFAAGSYYLNNMGNIKDLCLYNPLPLYHSAGGLVGIGIMMVFGVRIAIRRKFSVRNFWKDCIKYNCTGAQYIGEICRYLLSSPETPEDKSHNIQLLFGNGLRPTIWNQFTERFGVTRIAEFYGSTEGNANLMNIDCKPGAVGFASRLFPSVYPVRLVRLREDGNVLRDPRTGLCVLCKPGETGEFVGKIIKNHVMRSFDGYIDKEATEKKIVRNVLYQGDSWFRSGDLLNMDECGWMYFIDRIGDTFRWRGENVSTIEVEAVLSNVLQQRDNIVYGVEVPGAEGRAGMAXIV--GDELDLGGFLAAIKNQLPAYARPLFLRLVKSLDITGTFKLKKRCLQNEGYNPHVIQDPLYFLDTVKGVYRKLDAILYNDIMEMRCRI 639          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000012891 (supercontig:LSalAtl2s:LSalAtl2s981:104426:107346:-1 gene:EMLSAG00000012891 transcript:EMLSAT00000012891 description:"maker-LSalAtl2s981-augustus-gene-1.58")

HSP 1 Score: 457.218 bits (1175), Expect = 3.095e-154
Identity = 251/581 (43.20%), Postives = 359/581 (61.79%), Query Frame = 0

Query:    1 RVCLIHG---DKRWTFQDLEDYSNRVAHYFIRQ-SYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNM----QSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPD--------SVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVG-----FSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVL-HQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFL----QGVKKNLPAYATPIFIRLVE-QLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +V L++G   ++ WTF+ ++  S+RVA+YF      K GDCVAL+M N PE+I + LG +K+G++ AL+N++L    ++++++A +AKA I+G +L   +    +S++   PN  L  Y S S +    I   R  V+   DE+          S       +  + YIYTSGTTGLPK   I H R       +  L D+ S  +   Y  LPLYH++G  +G+G     G  VVI+KKFS RNFWKDC +Y     QYIGEICR+LL+    PEE QH + I++GNGLRP+IW  F  RF I  +GEFYG+TEGN N++N D+  GAVG         L   + P+ LI+VN ++  V RD +GLC+ C  GE GE +G+IVK HP + F+GY +      KI+R+VLK+GD++FRSGDLL  DE G++YF DR+GDTFRW+GENVST EVE   +++L +Q +  VYGV++ G++GRAGM A+  P+   D  K L     G K  LP YA P+F+RL+  ++ +T T+K+KK SL+ EGYD N++ D  F+Y + K  Y  L   +Y+E+   K++L
Sbjct:  133 KVMLVNGQNKEEEWTFEMIDQESSRVANYFSNNVGLKKGDCVALYMTNRPEFIYITLGLAKIGVIAALVNSHLRQDSMVYALKAGNAKALIYGIELQDAVDEMEKSLLDLSPNFTL--YCSKSGVSPEDITRKRSPVSKDFDEIVSKSPANRGASQNISLSPSSVIFYIYTSGTTGLPKPVKITHMRQMVILMAIKNLLDLNS--EDTSYCYLPLYHASGIQLGIGNAFANGGKVVIKKKFSARNFWKDCVKYKVTATQYIGEICRFLLATKPIPEETQHSIRIIYGNGLRPEIWRSFVERFKIPLVGEFYGSTEGNMNLINFDNTVGAVGCIPNIIPKFLIELITPIHLIKVNPQTLEVYRDENGLCVQCDVGEIGEGIGRIVKGHPVKEFNGYANEEENGNKILRDVLKKGDIFFRSGDLLVQDELGYLYFKDRVGDTFRWKGENVSTYEVEMTANSLLDNQTEVAVYGVKVSGMDGRAGMIAL--PSKDEDVKKLLDKLFDGFKSKLPLYARPVFVRLLHSEMALTATYKIKKFSLKTEGYDINLLKDDSFYY-ATKNGYLPLTQNVYDELSFGKIQL 706          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 82.4185 bits (202), Expect = 8.002e-17
Identity = 102/463 (22.03%), Postives = 178/463 (38.44%), Query Frame = 0

Query:    2 VCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQ------SIVHQIPNVELFSYGSD-SSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPV----FYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNG--LRPQIWTQFTNRFGIR-RIGEFYGATEGNSNVLNIDSVPGAV-GFSSVLFPFVYPVRLIRVNESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGV 449
            VC + G + +T++ +   S +     +R   K GD + L + N PE++  ++GC+ +G+    +N       +   ++   AK  +       N++      S + +I  + +    SD  S  E AI  D            D  +      D L   Y+SGTTG PK   + HS        + + +    I D V        LP +H       +   + FG  ++   KF    F     +Y       +  +  ++ S+P         ++++ G      P +  +F N+     R  E YG TE  S V ++    G + G    L P          N  G ++   +G  +   PGE GE    +  + P +   GY        KI++N       W  +GD+   ++ G  Y VDRL +  + +G  V+ +E+E ++       D    GV
Sbjct:   80 VCGMTG-RTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFF--------DQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTE-CSPVTHVQPKDGIIYGSCGNLIP----------NTIGKIIDIDTGKVL--GPGENGE----LCVSGP-QIMQGYYKNKKATDKIIQN------GWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGV 509          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 68.5514 bits (166), Expect = 2.923e-12
Identity = 107/404 (26.49%), Postives = 159/404 (39.36%), Query Frame = 0

Query:  147 NDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEI--MFGNGLRPQI-------------WTQFTNRFGIRRIGEFYGATE---GNSNVLNIDS-VPGAVGFSSVLFPFVYPVRLIRVNESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRL-GDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVK-KNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYD 529
            ND  L +YTSGT+G PK   I H       +GM          D      LPLYH  G I  + + +  GA V +   F  +  W      + N + ++G    Y L   E   +K++K EI  M  +  R  +             W   +    + R    YG TE   G  N L  D  +PG VG     FP V   R++  +E G+VL  S+ +       + GE   +     PS     Y +  AT++    N    GD WF +GD  +M+  G    + R   D  +  G  +S  E+E    +     +  V G+E   + G    A IV   G    +  L+G   K LP Y+ P    LV  +D     K+ K  L++    P+ + +     D
Sbjct:  147 NDGALILYTSGTSGSPKGVWITHRNIEAQVSGMIKF--WAWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLTNSSMNISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRKWQTISGHILLER----YGMTEIGMGLGNSLEEDKRLPGFVG---TPFPNV-EARIM--SEEGSVLAYSNDVEFSYLSDKSGELQIR----GPSVFSFYYGNEKATKESFDPN----GD-WFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLE-DEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYSVPRQFMLVNSIDKNHMGKINKKELKKMDSPPDPLKESDILSD 528          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000010073 (supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10")

HSP 1 Score: 52.7582 bits (125), Expect = 1.758e-7
Identity = 43/202 (21.29%), Postives = 86/202 (42.57%), Query Frame = 0

Query:    7 GDKRW-TFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSD---SSIREGAIKLDRELVNSSIDEV---PDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYH 201
            G+ +W +F ++E+ S            +  + + +F  +  E++   LGC K       + ANL    + H +        I   DL P +++++ + P+++   +  D   ++ R G     R L    + E+    ++       +D  + +YTSG+TG+PK  ++ H       A  ++L     I +  +   LPL H
Sbjct:  138 GEYQWKSFNEIEELSTDFGKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTHDLLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVIELGSKSETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGTIAA-FHLVVKDKIPEETYLGYLPLAH 338          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000006577 (supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-1 gene:EMLSAG00000006577 transcript:EMLSAT00000006577 description:"maker-LSalAtl2s360-snap-gene-1.18")

HSP 1 Score: 51.9878 bits (123), Expect = 3.336e-7
Identity = 99/457 (21.66%), Postives = 167/457 (36.54%), Query Frame = 0

Query:   33 KPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACI-----FGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSID--------------EVPDSVKNCTHFN--DKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMY-YLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIR-KKFSVRN--FWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQH----KVEIMFGNGLRPQIWTQFTNRFGIRR--IGEFYGATEGNSNVLNIDSVPGAVGFS--SVLFPFVYPVRLIRVNESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHP----SRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIP 452
            + GD VA++M    E +   L C+++G V +++        L   I    AK  I     +  +   N+  + ++       +Y  D  I      + R    S ++              EV +   +    N  D L  +YTSG+TG PK  +   + +  Y A  + Y+ D     D  +      + +    V  G ++    +V+     F   N  FW+   +Y+ +         R L+   E    K      KV    G  + P+ W  +    G  +  I + Y  TE   +++    +PG       S  FPF + V  + ++ESG VL               GE  G IV N P     RS  G        ++  +        ++ SGD    D+ G+++   R+ D     G  +ST +VE+ L    H  +  V     P
Sbjct:   80 QKGDRVAIYMPMIIELVVAMLACARIGAVHSIVFGGYSSDSLASRIVDGGAKVLISADGVYRGNKLINLLDVAYKAMEETSSNYNHDIDIHILVSHIPRLNSGSPLETPSRWKTERDHWWHEVMEPASDVEWMNSEDPLFLLYTSGSTGKPKGVLHTTAGYMIYAATTFKYVFDYQDESDVYWCTADIGWITGHTYVTYGPLLNGSKSVLFEGTPFYPTNDRFWEIIQKYSVSKFYTAPTAIRALMKFGEDFVSKYDLSSLKVLGTVGEPINPEAWFWYHKNVGGSQCSIVDTYWQTETGGHIMT--PLPGCTPTKPGSACFPF-FGVSPVILDESGKVLS--------------GEAEGYIVFNKPWPGMMRSVYG------NHERFEKTYFSRFPGYYMSGDGAKRDKDGYIWITGRIDDMLNCSGHLMSTAQVESALVEHRHVAEAAVVSTPHP 513          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|74749065|sp|Q6P1M0.1|S27A4_HUMAN (RecName: Full=Long-chain fatty acid transport protein 4; Short=FATP-4; Short=Fatty acid transport protein 4; AltName: Full=Solute carrier family 27 member 4)

HSP 1 Score: 548.51 bits (1412), Expect = 0.000e+0
Identity = 270/550 (49.09%), Postives = 364/550 (66.18%), Query Frame = 0

Query:    8 DKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQI-PNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTH--FNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            D  WTF+ L++YS+ VA++   +    GD  A+FM N  E++GLWLG +KLG+  ALIN NL    LLH +    A+A +FG+++   +  +   + P++ LF  GS      GA+    E ++  + + P  + +C    F DKL YIYTSGTTGLPKAA++ HSR+Y   A +YY   M    + + YD LPLYHSAG IVG+G  ++ G TVVIRKKFS   FW DC +YNC   QYIGE+CRYLL+ P    E QH+V +  GNGLR  IWT F++RF I ++ EFYGATE N ++ N DS  GA GF+S +  FVYP+RL+RVNE +  ++R   G+CI CQPGEPG+ VG+I++  P R FDGY++  A  KKI ++V K+GD  + +GD+L MDE G++YF DR GDTFRW+GENVST EVE  LS +L   D  VYGVE+PG EGRAGMAA+  PTG  D  +F Q ++K LP YA PIF+RL+ +L  TGT+K +K  L++EG+DP ++ DPLF+ D+ KG Y  LD   Y+ I   + +L
Sbjct:   99 DTHWTFRQLDEYSSSVANFLQARGLASGDVAAIFMENRNEFVGLWLGMAKLGVEAALINTNLRRDALLHCLTTSRARALVFGSEMASAICEVHASLDPSLSLFCSGS---WEPGAVPPSTEHLDPLLKDAPKHLPSCPDKGFTDKLFYIYTSGTTGLPKAAIVVHSRYYRMAALVYYGFRMRP--NDIVYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYNCTIVQYIGELCRYLLNQPPREAENQHQVRMALGNGLRQSIWTNFSSRFHIPQVAEFYGATECNCSLGNFDSQVGACGFNSRILSFVYPIRLVRVNEDTMELIRGPDGVCIPCQPGEPGQLVGRIIQKDPLRRFDGYLNQGANNKKIAKDVFKKGDQAYLTGDVLVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLDMADVAVYGVEVPGTEGRAGMAAVASPTGNCDLERFAQVLEKELPLYARPIFLRLLPELHKTGTYKFQKTELRKEGFDPAIVKDPLFYLDAQKGRYVPLDQEAYSRIQAGEEKL 643          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|565683208|sp|Q4R3Y4.2|S27A4_MACFA (RecName: Full=Long-chain fatty acid transport protein 4; Short=FATP-4; Short=Fatty acid transport protein 4; AltName: Full=Solute carrier family 27 member 4)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 269/550 (48.91%), Postives = 363/550 (66.00%), Query Frame = 0

Query:    8 DKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQI-PNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTH--FNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            D  WTF+ L++YS+ VA++   +    GD  A+FM N  E++GLWLG +KLG+  ALIN NL    LLH +    A+A +FG+++   +  I   + P++ LF  GS       A+    E ++  +++ P  + +C    F DKL YIYTSGTTGLPKAA++ HSR+Y   A +YY   M    + + YD LPLYHSAG IVG+G  ++ G TVVIRKKFS   FW DC +Y C   QYIGE+CRYLL+ P    E QH+V +  GNGLR  IWT F++RF I ++ EFYGATE N ++ N DS  GA GF+S +  FVYP+RL+RVNE +  ++R   G+CI CQPGEPG+ VG+I++  P R FDGY++  A  KKI ++V K+GD  + +GD+L MDE G++YF DR GDTFRW+GENVST EVE  LS +L   D  VYGVE+PG EGRAGMAA   PTG  D  +F Q ++K LP YA PIF+R++ +L  TGT+KL+K  L++EG+DP ++ DPLF+ D+ KG Y  LD   Y+ I   + +L
Sbjct:   99 DTHWTFRQLDEYSSSVANFLQARGLASGDVAAIFMENRNEFVGLWLGMAKLGVEAALINTNLRRDALLHCLTTSRARALVFGSEMASAICEIHASLDPSLSLFCSGS---WEPNAVPTSTEHLDPLLEDAPKHLPSCPDKGFTDKLFYIYTSGTTGLPKAAIVVHSRYYRMAALVYYGFRMRP--NDIIYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYKCTIVQYIGELCRYLLNQPPREAENQHQVRMALGNGLRQSIWTNFSSRFHIPQVAEFYGATECNCSLGNFDSQVGACGFNSRILSFVYPIRLVRVNEDTMELIRGPDGICIPCQPGEPGQLVGRIIQKDPLRRFDGYLNQGANNKKIAKDVFKKGDQAYLTGDVLVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLDMADVAVYGVEVPGTEGRAGMAAGASPTGNCDLERFAQDLEKELPLYARPIFLRILPELHKTGTYKLQKTELRKEGFDPAIVKDPLFYLDARKGRYVLLDQEAYSRIQAGEEKL 643          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|81902280|sp|Q91VE0.1|S27A4_MOUSE (RecName: Full=Long-chain fatty acid transport protein 4; Short=FATP-4; Short=Fatty acid transport protein 4; AltName: Full=Solute carrier family 27 member 4)

HSP 1 Score: 543.888 bits (1400), Expect = 0.000e+0
Identity = 269/550 (48.91%), Postives = 360/550 (65.45%), Query Frame = 0

Query:    8 DKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQI-PNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTH--FNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            D  WTF+ L++YS+ VA++   +    G+ VALFM N  E++GLWLG +KLG+  ALIN NL    L H ++   A+A IFG+++   +  I   + P + LF  GS        + +  E ++  +++ P  + +     F DKL YIYTSGTTGLPKAA++ HSR+Y   + +YY   M    D + YD LPLYHSAG IVG+G  ++ G TVVIRKKFS   FW DC +YNC   QYIGE+CRYLL+ P    E +HKV +  GNGLR  IWT F++RF I ++ EFYGATE N ++ N DS  GA GF+S +  FVYP+RL+RVNE +  ++R   G+CI CQPG+PG+ VG+I++  P R FDGY++  A  KKI  +V K+GD  + +GD+L MDE G++YF DR GDTFRW+GENVST EVE  LS +LH  D  VYGVE+PG EGRAGMAA+  P    D   F Q +KK LP YA PIF+R + +L  TGTFK +K  L++EG+DP+V+ DPLF+ D+ KG Y +LD   Y  I   + +L
Sbjct:   99 DTHWTFRQLDEYSSSVANFLQARGLASGNVVALFMENRNEFVGLWLGMAKLGVEAALINTNLRRDALRHCLDTSKARALIFGSEMASAICEIHASLEPTLSLFCSGS---WEPSTVPVSTEHLDPLLEDAPKHLPSHPDKGFTDKLFYIYTSGTTGLPKAAIVVHSRYYRMASLVYYGFRMRP--DDIVYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYNCTIVQYIGELCRYLLNQPPREAESRHKVRMALGNGLRQSIWTDFSSRFHIPQVAEFYGATECNCSLGNFDSRVGACGFNSRILSFVYPIRLVRVNEDTMELIRGPDGVCIPCQPGQPGQLVGRIIQQDPLRRFDGYLNQGANNKKIANDVFKKGDQAYLTGDVLVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLHMADVAVYGVEVPGTEGRAGMAAVASPISNCDLESFAQTLKKELPLYARPIFLRFLPELHKTGTFKFQKTELRKEGFDPSVVKDPLFYLDARKGCYVALDQEAYTRIQAGEEKL 643          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|75061982|sp|Q5RDY4.1|S27A4_PONAB (RecName: Full=Long-chain fatty acid transport protein 4; Short=FATP-4; Short=Fatty acid transport protein 4; AltName: Full=Solute carrier family 27 member 4)

HSP 1 Score: 543.117 bits (1398), Expect = 0.000e+0
Identity = 269/550 (48.91%), Postives = 362/550 (65.82%), Query Frame = 0

Query:    8 DKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQI-PNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTH--FNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            D  WTF+ L++YS+ VA++   +    GD  A+FM N  E++GLWLG +KLG+  ALIN NL     LH +    A+A +FG+++   +  I   + P++ LF  GS       A+    E ++  + + P  +  C    F DKL YIYTSGTTGLPKAA++ HSR+Y   A +YY   M    + + YD LPLYHSAG IVG+G  ++ G TVVIRKKFS   FW DC +YNC   QYIGE+CRYLL+ P    E QH+V +  GNGLR  IWT F++RF I ++ EFYGATE N ++ N DS  GA GF+S +  FVYP+RL+RVNE +  ++R   G+CI CQPGEPG+ VG+I++  P R FDGY++  A +KKI ++V K+GD  + +GD+L MDE G++YF DR GDTFRW+GENVST EVE  LS +L   D  VYGVE+PG EGRAGMAA+  PTG  D  +F Q ++K LP YA PIF+RL+ +L  TGT+K +K  L++EG+DP ++ DPLF+ D+ KG Y  LD   Y+ I   + +L
Sbjct:   99 DTLWTFRQLDEYSSSVANFLQARGLASGDVAAIFMENRNEFVGLWLGMAKLGVEAALINTNLRRDAQLHCLTTSRARALVFGSEMASAICEIHASLDPSLSLFCSGS---WEPNAVPTSTEHLDPLLKDAPKHLPICPDKGFTDKLFYIYTSGTTGLPKAAIVVHSRYYRMAALVYYGFRMRP--NDIVYDCLPLYHSAGNIVGIGQCLLHGMTVVIRKKFSASRFWDDCIKYNCTIVQYIGELCRYLLNQPPREAENQHQVRMALGNGLRQSIWTNFSSRFHIPQVAEFYGATECNCSLGNFDSQVGACGFNSRILSFVYPIRLVRVNEDTMELIRGPDGICIPCQPGEPGQLVGRIIQKDPLRRFDGYLNQGANDKKIAKDVFKKGDQAYLTGDVLVMDELGYLYFRDRTGDTFRWKGENVSTTEVEGTLSRLLDMADVAVYGVEVPGTEGRAGMAAVASPTGNCDLERFAQVLEKELPLYARPIFLRLLPELHKTGTYKFQKTELRKEGFDPAIVKDPLFYLDARKGRYVPLDQEAYSRIQAGEEKL 643          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|2492888|sp|P97849.1|S27A1_RAT (RecName: Full=Long-chain fatty acid transport protein 1; Short=FATP-1; Short=Fatty acid transport protein 1; AltName: Full=Solute carrier family 27 member 1)

HSP 1 Score: 540.806 bits (1392), Expect = 0.000e+0
Identity = 273/540 (50.56%), Postives = 359/540 (66.48%), Query Frame = 0

Query:   11 WTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDS---VKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEI 546
            WTF  L+ YSN VA+ F++  + PGD VA+F+   PE++GLWLG +K G+V AL+N NL   PL   +   +AKA I+G ++   +  +  Q+    L  + S     E  +  D +L++  + E P +          +D+L YIYTSGTTGLPKAA++ HSR+Y   A  ++   M +  + V YD LPLYHSAG I+GVG  +++G TVV+RKKFS   FW DC +YNC   QYIGEICRYLL  P    E++H+V +  GNGLRP IW +FT  FG+R+IGEFYGATE N ++ N+D   G+ GF+S +   VYP+RL++VNE +   LRDS GLCI CQPGEPG  VG+I +  P R FDGYV  +AT KKI  +V ++GD  + SGD+L MDE G+MYF DR GDTFRWRGENVST EVEAVLS +L Q D  VYGV +PGVEG++GMAAI +P  +LD     Q ++K L +YA PIF+RL+ Q+D TGTFK++K  LQREG+DP   SD LFF D  +G Y  LD  ++  I
Sbjct:  104 WTFAQLDTYSNAVANLFLQLGFAPGDVVAVFLEGRPEFVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGK-SLLKFCSGDLGPESVLP-DTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRA--NDVLYDCLPLYHSAGNIMGVGQCIIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVRDVERRHRVRLAVGNGLRPAIWEEFTQGFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIRLVKVNEDTMEPLRDSQGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKKIAHSVFRKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEAVLSRLLGQTDVAVYGVAVPGVEGKSGMAAIADPHNQLDPNSMYQELQKVLASYAQPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGRYLPLDERVHARI 639          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|2492887|sp|Q60714.1|S27A1_MOUSE (RecName: Full=Long-chain fatty acid transport protein 1; Short=FATP-1; Short=Fatty acid transport protein 1; AltName: Full=Solute carrier family 27 member 1)

HSP 1 Score: 538.88 bits (1387), Expect = 0.000e+0
Identity = 275/540 (50.93%), Postives = 358/540 (66.30%), Query Frame = 0

Query:   11 WTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDS---VKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEI 546
            WTF  L+ YSN VA+ F +  + PGD VA+F+   PE++GLWLG +K G+V AL+N NL   PL   +   +AKA I+G ++   +  +  Q+    L  + S     E +I  D +L++  + E P +          +D+L YIYTSGTTGLPKAA++ HSR+Y   A  ++   M +    V YD LPLYHSAG I+GVG  +++G TVV+RKKFS   FW DC +YNC   QYIGEICRYLL  P    E++H+V +  GNGLRP IW +FT RFG+ +IGEFYGATE N ++ N+D   G+ GF+S +   VYP+RL++VNE +   LRDS GLCI CQPGEPG  VG+I +  P R FDGYV  +AT KKI  +V ++GD  + SGD+L MDE G+MYF DR GDTFRWRGENVST EVEAVLS +L Q D  VYGV +PGVEG+AGMAAI +P  +LD     Q ++K L +YA PIF+RL+ Q+D TGTFK++K  LQREG+DP   SD LFF D  +G Y  LD  ++  I
Sbjct:  104 WTFAQLDTYSNAVANLFRQLGFAPGDVVAVFLEGRPEFVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGK-SLLKFCSGDLGPE-SILPDTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRAAD--VLYDCLPLYHSAGNIMGVGQCVIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVRDVEQRHRVRLAVGNGLRPAIWEEFTQRFGVPQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIRLVKVNEDTMEPLRDSEGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKKIAHSVFRKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEAVLSRLLGQTDVAVYGVAVPGVEGKAGMAAIADPHSQLDPNSMYQELQKVLASYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGRYVPLDERVHARI 639          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|74749156|sp|Q6PCB7.1|S27A1_HUMAN (RecName: Full=Long-chain fatty acid transport protein 1; Short=FATP-1; Short=Fatty acid transport protein 1; AltName: Full=Solute carrier family 27 member 1)

HSP 1 Score: 538.495 bits (1386), Expect = 0.000e+0
Identity = 273/540 (50.56%), Postives = 352/540 (65.19%), Query Frame = 0

Query:   11 WTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEV---PDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEI 546
            WTF  L+ YSN VA+ F +  + PGD VA+F+   PE++GLWLG +K GM  AL+N NL   PL   +    AKA IFG ++   +  +   +    L  + S     EG +  D  L++  + E    P +       +D+L YIYTSGTTGLPKAA++ HSR+Y   A  ++   M +    V YD LPLYHSAG I+GVG  +++G TVV+RKKFS   FW DC +YNC   QYIGEICRYLL  P    E++H+V +  GNGLRP IW +FT RFG+R+IGEFYGATE N ++ N+D   G+ GF+S + P VYP+RL++VNE +  +LRD+ GLCI CQ GEPG  VG+I +  P R FDGYV  +AT KKI  +V  +GD  + SGD+L MDE G+MYF DR GDTFRWRGENVST EVE VLS +L Q D  VYGV +PGVEG+AGMAA+ +P   LD     Q ++K L  YA PIF+RL+ Q+D TGTFK++K  LQREG+DP   SD LFF D  +G Y  L+  +Y  I
Sbjct:  104 WTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPEFVGLWLGLAKAGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGK-SLIKFCSGDLGPEGILP-DTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRMAAFGHHAYRMQAAD--VLYDCLPLYHSAGNIIGVGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVREAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQAGEPGLLVGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKAGMAAVADPHSLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGHYLPLNEAVYTRI 639          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|81170471|sp|Q3ZKN0.1|S27A1_BOVIN (RecName: Full=Long-chain fatty acid transport protein 1; Short=FATP-1; Short=Fatty acid transport protein 1; AltName: Full=Solute carrier family 27 member 1)

HSP 1 Score: 537.339 bits (1383), Expect = 0.000e+0
Identity = 274/540 (50.74%), Postives = 352/540 (65.19%), Query Frame = 0

Query:   11 WTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEV---PDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEI 546
            WTF  L+ YSN VA+ F R  + PGD VA+FM   PE++GLWLG +K G+  AL+N NL   PL   +    AKA +FG +L   +  +  ++    L  + S     +G    D +L++  + E    P +       +D+L YIYTSGTTGLPKAA+I HSR+Y   A  +Y   M +    V YD LPLYHSAG I+GVG  +++G TVV+RKKFS   FW DC +YNC   QYIGEICRYLL  P    E +H+V +  GNGLRP IW +FT RFG+R+IGEFYGATE N ++ N+D   G+ GF+S + P VYP+RL++VNE +  +LRD+ GLCI CQ GEPG  VG+I +  P R FDGY+  +AT KKI  +V ++GD  + SGD+L MDE G+MYF DR GDTFRWRGENVST EVE VLS +L Q D  VYGV +PGVEG+A MAAI +P GRL      + ++K L  YA PIF+RL+ Q+D TGTFK++K  LQ EG+DP   SD LFF D  +G Y  LD  +Y  I
Sbjct:  104 WTFAQLDAYSNAVANLFRRLGFAPGDVVAIFMEGRPEFVGLWLGLAKAGVEAALLNVNLRREPLAFCLGTSGAKALVFGGELAAAVAEMSGELGK-SLVKFCSGDVGPDGVFP-DTQLLDPLLKETSTAPLAQPPGKGMDDRLFYIYTSGTTGLPKAAIIVHSRYYRIAAFGHYSYSMQAAD--VLYDCLPLYHSAGNIMGVGQCLIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLKQPVREAEGRHRVRLAVGNGLRPSIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSRGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQTGEPGLLVGQINQQDPLRRFDGYISESATSKKIAHSVFRKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKACMAAIADPHGRLSPNALYEELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQHEGFDPRQTSDRLFFLDLKQGHYLPLDQGVYTRI 639          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|74725713|sp|Q9Y2P4.1|S27A6_HUMAN (RecName: Full=Long-chain fatty acid transport protein 6; Short=FATP-6; Short=Fatty acid transport protein 6; AltName: Full=Fatty-acid-coenzyme A ligase, very long-chain 2; AltName: Full=Solute carrier family 27 member 6; AltName: Full=Very long-chain acyl-CoA synthetase homolog 1; Short=VLCSH1; Short=hVLCS-H1)

HSP 1 Score: 400.208 bits (1027), Expect = 9.085e-132
Identity = 218/554 (39.35%), Postives = 325/554 (58.66%), Query Frame = 0

Query:    4 LIHGDKRWTFQDLEDYSNRVAHYFIRQS-YKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDP-AATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIV-EPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +I+    +T+QD++  S+RVAH F+  S  K GD VAL M N P+++ +W G +KLG V A +N N+    LL+ I A   +A + GADL   ++ I+  +    +  +G   S+ +G I L  +L  S  + VP S    +      LYI+TSGTTGLPKAAVI   +      G   L   G     + Y  LPLYHS+  I+G+   +  GAT V++KKFS   FW DC +Y+    QYIGE+CRYL    +   EK HKV +  GNG+R  +W +F +RFG  ++ E Y ATE + + +N     GA+G +++ +  +    LI+ + +    +R+  G CI  + GEPG  + ++   +P   F GY  P   T+ K++ +V K+GD++  +GDL+  D+  ++YF DR GDTFRW+GENV+T EV  V+  +   Q+  VYGV I G EGRAGMA+I+ +P   LD  K  + V   LPAYA P F+R+ E+++ TGTFKL K  L  +G++P  IS+PL+F D++K +Y  L   LY++IM  +++L
Sbjct:   73 IIYEGDIYTYQDVDKRSSRVAHVFLNHSSLKKGDTVALLMSNEPDFVHVWFGLAKLGCVVAFLNTNIRSNSLLNCIRACGPRALVVGADLLGTVEEILPSLSE-NISVWGMKDSVPQGVISLKEKLSTSPDEPVPRSHHVVSLLKSTCLYIFTSGTTGLPKAAVISQLQV---LRGSAVLWAFGCTAHDIVYITLPLYHSSAAILGISGCVELGATCVLKKKFSASQFWSDCKKYDVTVFQYIGELCRYLCKQSKREGEKDHKVRLAIGNGIRSDVWREFLDRFGNIKVCELYAATESSISFMNYTGRIGAIGRTNLFYKLLSTFDLIKYDFQKDEPMRNEQGWCIHVKKGEPGLLISRVNAKNP---FFGYAGPYKHTKDKLLCDVFKKGDVYLNTGDLIVQDQDNFLYFWDRTGDTFRWKGENVATTEVADVIGMLDFIQEANVYGVAISGYEGRAGMASIILKPNTSLDLEKVYEQVVTFLPAYACPRFLRIQEKMEATGTFKLLKHQLVEDGFNPLKISEPLYFMDNLKKSYVLLTRELYDQIMLGEIKL 619          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|308153494|sp|O14975.2|S27A2_HUMAN (RecName: Full=Very long-chain acyl-CoA synthetase; Short=VLACS; Short=VLCS; AltName: Full=Fatty acid transport protein 2; Short=FATP-2; AltName: Full=Fatty-acid-coenzyme A ligase, very long-chain 1; AltName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Solute carrier family 27 member 2; AltName: Full=THCA-CoA ligase; AltName: Full=Very long-chain-fatty-acid-CoA ligase)

HSP 1 Score: 399.823 bits (1026), Expect = 1.335e-131
Identity = 228/559 (40.79%), Postives = 323/559 (57.78%), Query Frame = 0

Query:    4 LIHGDKRWTFQDLEDYSNRVA-----HYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVEL-FSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAA-TEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAI-VEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            L+  D+  T+  ++  SN+VA     H  +RQ    GDCVAL MGN P Y+ LWLG  KLG   A +N N+  + LLH  +   AK  +   +L   ++ I+  +   ++   Y S +S  +G      ++   S + +P+S ++   F+   LYIYTSGTTGLPKAA+I H R + Y  G+ +++ + +  D V Y  LP YHSA  ++G+   +V GAT+ +R KFS   FW DC +YN    QYIGE+ RYL ++P+ P ++ HKV +  GNGLR  +W QF  RFG   I EFY ATEGN   +N     GAVG  + L   +    LI+ + E    +RD +G C+    GE G  V KI +  P   F+GY    A TEKK +R+V K+GD++F SGDLL +D   ++YF DR+GDTFRW+GENV+T EV   +  V   Q+  VYGV +P  EGR GMA+I ++     D  K  Q +   LP+YA P F+R+ + ++ITGTFK +KM+L  EG++P VI D L+F D     Y  +   +YN I    L+L
Sbjct:   72 LLFRDETLTYAQVDRRSNQVARALHDHLGLRQ----GDCVALLMGNEPAYVWLWLGLVKLGCAMACLNYNIRAKSLLHCFQCCGAKVLLVSPELQAAVEEILPSLKKDDVSIYYVSRTSNTDGIDSFLDKVDEVSTEPIPESWRSEVTFSTPALYIYTSGTTGLPKAAMITHQRIW-YGTGLTFVSGLKA--DDVIYITLPFYHSAALLIGIHGCIVAGATLALRTKFSASQFWDDCRKYNVTVIQYIGELLRYLCNSPQKPNDRDHKVRLALGNGLRGDVWRQFVKRFGDICIYEFYAATEGNIGFMNYARKVGAVGRVNYLQKKIITYDLIKYDVEKDEPVRDENGYCVRVPKGEVGLLVCKITQLTP---FNGYAGAKAQTEKKKLRDVFKKGDLYFNSGDLLMVDHENFIYFHDRVGDTFRWKGENVATTEVADTVGLVDFVQEVNVYGVHVPDHEGRIGMASIKMKENHEFDGKKLFQHIADYLPSYARPRFLRIQDTIEITGTFKHRKMTLVEEGFNPAVIKDALYFLDDTAKMYVPMTEDIYNAISAKTLKL 620          

BLAST of amp dependent ligase vs. nr
Match: gi|1325278900|ref|XP_023324091.1| (long-chain fatty acid transport protein 4-like [Eurytemora affinis])

HSP 1 Score: 776.548 bits (2004), Expect = 0.000e+0
Identity = 362/557 (64.99%), Postives = 444/557 (79.71%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDS--SIREG--AIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            RVCL   D+ WTF+ L++YSN++A++ +   +K G  ++LFM N PEY+G WLGCSK+G+VPALIN NL G+ L+HSI A ++ A IFG +L   +  +   + N+ L+S GS S  + R       LDREL N S  +VP+SVK C  F DKLLYIYTSGTTGLPKAAV+K+SRFYFYC G+YY+N M  + + VFYDPLPLYHSAGG+VG+GLMM FGATVV+R+KFSVRNFWKDC +YNCNGAQYIGEICRYLLS PE+PEE+ H+VEIMFGNGLRPQIW QF +RFG++ IGEFYGATEGNSNVLNIDS PG+VGF+SVLFP VYPV +IRV++ GN +RD  GLCI C PGEPGEFVGKI+K HPSR FDGYVD  ATE KIV +VL+ GDM+ +SGDLL  DEFGWMYF+DR+GDT+RWRGENVST EVE+V+S+VL Q D++VYGV+IPGVEG+AGMAAIVE   + D  KFL+ +K  LP YA P+FIRLV  +D+TGTFK++K+ L REGYDP +ISDPLFFY++  G+Y  LDTCLY +I+  +  L
Sbjct:   90 RVCLHFEDESWTFKQLDEYSNKIANFLLSAGFKHGQSISLFMENRPEYVGTWLGCSKIGVVPALINFNLRGQSLIHSILASTSVAVIFGTELAEAISEVSEDLENIRLYSSGSSSRPTTRRSFHTCSLDRELQNHSHHDVPESVKKCLSFTDKLLYIYTSGTTGLPKAAVVKNSRFYFYCGGVYYMNAMYRMNELVFYDPLPLYHSAGGVVGIGLMMCFGATVVLRQKFSVRNFWKDCIKYNCNGAQYIGEICRYLLSVPESPEERNHRVEIMFGNGLRPQIWQQFVDRFGVKYIGEFYGATEGNSNVLNIDSKPGSVGFTSVLFPSVYPVSIIRVDQEGNPVRDERGLCIRCNPGEPGEFVGKIIKGHPSRGFDGYVDKKATENKIVHSVLRHGDMYCKSGDLLYADEFGWMYFMDRMGDTYRWRGENVSTVEVESVISSVLEQTDSVVYGVDIPGVEGKAGMAAIVEDVAKFDLEKFLKRIKAELPIYAIPLFIRLVGTIDLTGTFKIRKLDLVREGYDPKIISDPLFFYNAAVGSYCKLDTCLYEDIVSKRCHL 646          

BLAST of amp dependent ligase vs. nr
Match: gi|1061471232|gb|ODM90306.1| (Long-chain fatty acid transport protein 4 [Orchesella cincta])

HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0
Identity = 299/576 (51.91%), Postives = 409/576 (71.01%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSI--VHQIPNVELFSYGS---DSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSN----------------VLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRD-SSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VC    ++ W F+D+++ SNRVA++F  Q  K GDCV++FM N PEY+ +WLG SK+G+VPALIN +L  +PL HSIE   +KA IFG +L   +Q +  +  + ++ +F+ G    DS + + A  LD EL NS I   P  +    +F DKLLYIYTSGTTGLPKAA+IK+SRF F+  G++  N MG  ++ V YDPLPLYH+AGG++GVG  ++FG T V+R+KFS R ++KDC +YNC+ AQYIGEICRYLLS P +P++  H + +MFGNGLRPQIW  F+ RF I+ I EFYGATEGN N                V+NID+  G+VGF S++ PF+YPV LIR++E +G  +RD  +GL + C+P EPGE VGKI++NHP R F+GY D  AT KKIVR V  + D  FR+GD+L MDEFG+ YF DR GDTFRW+GEN ST EVEAV+ NV   +D++VYGVEIPG +GRAGMAAI++P  ++D +    GV K LP+YA P+F+R+V+Q+D+TGT+K+KKM LQ+EG+D   +S+ ++F+    G Y  LD  LY++I++ ++R+
Sbjct:   96 KVCFYFEEEVWKFKDVDEASNRVANFFASQGLKKGDCVSVFMENRPEYVCVWLGLSKIGIVPALINFHLRLQPLKHSIEVAKSKALIFGRELGGAIQDVQDLGLVSDLPMFATGKGEPDSVVIKDARDLDSELENSPIS--PPKLTEKINFTDKLLYIYTSGTTGLPKAAIIKNSRFLFFTMGIH--NFMGISKNEVIYDPLPLYHTAGGMLGVGQALIFGCTAVLRRKFSARAYFKDCAKYNCSAAQYIGEICRYLLSTPPSPDDTNHHIRMMFGNGLRPQIWETFSKRFNIKTISEFYGATEGNCNISKEHNIMGFMPSSQVVVNIDNHIGSVGFISMIAPFIYPVTLIRIDEATGEPIRDPKTGLVMRCKPDEPGELVGKIIENHPVREFEGYADSTATTKKIVRGVFNKTDAVFRTGDVLVMDEFGYFYFKDRTGDTFRWKGENCSTAEVEAVVMNVCGLKDSVVYGVEIPGTDGRAGMAAILDPEEKVDMSSLAAGVTKALPSYARPLFVRIVKQIDMTGTYKMKKMDLQKEGFDVTRVSEKIYFFSG--GKYVLLDNELYSKIVKGEVRV 665          

BLAST of amp dependent ligase vs. nr
Match: gi|1228377255|ref|XP_021949971.1| (long-chain fatty acid transport protein 4-like [Folsomia candida])

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 295/558 (52.87%), Postives = 393/558 (70.43%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVH--QIPNVELFSYG-SDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNE-SGNVLRD-SSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VC    D+ W+F+D+++ SNR+A+YF  Q  + G+CV++FM N P Y+ +WLG SK+G++PALIN NL  +PL H  E    KA IFG +L   +  ++    +P  +L+S G  D  +   ++ LD + + S   E P  +    +F+DKLLYI+TSGTTGLPKAA+IKHSRF F+  G++Y   +G   D V YDPLPLYH+AGG++GVG  ++ G T V+R+KFS  +++KDC  YNC  AQYIGEICRYLL  P    +K H+V +MFGNGLRPQIW +F +RF I  I EFYGATEGN N++NID+  G+VGF S++ PF+YPV LIRV+E +G V+RD  +GL + C+P EPGE VGKIV NHP R FDGY D  AT KKIV +V ++GD  FR+GD+L MDE G+ YF DR GDTFRW+GEN ST EVEAV+SNV   +D + YGVEIP  +GRAGMAAI++P   LDF     GV K+LP+YA P+F+R+V+ LD+TGT+K+KK+ LQ+EG+D   ISD +FF     G Y  +D  LY++I+   LRL
Sbjct:  302 KVCFYFEDEVWSFRDIDEESNRIANYFASQGLQKGECVSVFMENRPMYVCIWLGLSKIGVIPALINFNLRQQPLKHCFEVAKPKAIIFGHELGLAINEVIELGPLPKCDLYSTGHGDEQLLFNSLDLDVK-IKSYSKEAP-VISTPINFSDKLLYIFTSGTTGLPKAAIIKHSRFIFFTMGIHYF--VGISNDEVIYDPLPLYHTAGGMIGVGQALIHGCTAVLRRKFSASSYFKDCARYNCTAAQYIGEICRYLLCQPPNSSDKNHQVRLMFGNGLRPQIWEKFASRFNISIISEFYGATEGNCNIINIDNHVGSVGFISIILPFIYPVTLIRVDEATGEVMRDPRTGLVLRCKPNEPGELVGKIVPNHPVREFDGYADSNATTKKIVTDVFRKGDAAFRTGDVLIMDECGYFYFKDRTGDTFRWKGENCSTAEVEAVVSNVCGLKDAVCYGVEIPFTDGRAGMAAILDPDASLDFDSLAHGVTKSLPSYARPLFVRIVKHLDMTGTYKMKKIDLQKEGFDVTKISDGIFFL--CNGKYVRMDNELYSKIISGYLRL 853          

BLAST of amp dependent ligase vs. nr
Match: gi|675373448|gb|KFM66350.1| (Long-chain fatty acid transport protein 1, partial [Stegodyphus mimosarum])

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 292/556 (52.52%), Postives = 396/556 (71.22%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIP--NVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNES-GNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            + CL   D++WTFQ +EDYSN++A+ F +  +  GD VA+FM + PE++G+WLG SK+G+VPALIN+NL   PL HSI  V+ KA IFG++LT  ++ +   +   N++ F  G  S     A  L+  +  SSI   P   +N  + +D+L Y+YTSGTTG+PKAA+I+HSRF +  AG++Y+  + S    V Y  LPLYH+AGGI+ +   ++FG T+ IRKKFS   FW DC +Y    AQYIGE+CRYLL+ PE P+E++H+V +MFGNGLRPQIW +F +RF I  IGE YG+TEGN+NV+NID+  GAVGF S + P +YPV LI+V  S G  +RD  GLCI C+PGEPGEFVGKI+   P R FDGYV+ +AT+KKIVR+  ++GDM F SGD+L MDE G++YFVDR GDTFRWRGENVST EVE V+SN+L   D++VYGV +P VEGRAGMAAI +P   +D    L  + K LP+YA P FIR+ ++L+ TGTFKLKK+ LQ+EG+DP+++ DPL++ +    AY  LD  +Y++I   ++R+
Sbjct:   58 KTCLWMEDEKWTFQQVEDYSNQIANCFAKLGFHRGDDVAVFMESKPEFVGIWLGLSKIGVVPALINSNLRLDPLAHSITVVNCKAVIFGSELTDAVKGVSSMLKDVNMQYFCLGECSDGDIPARSLNSLIQESSIS--PPPTQNKGNMDDRLFYVYTSGTTGMPKAAIIRHSRFMWLGAGIHYMILLKS--KDVIYTALPLYHTAGGILSLSQTILFGNTLAIRKKFSASKFWDDCIKYQATVAQYIGELCRYLLAQPENPQERKHQVRLMFGNGLRPQIWRKFVDRFNIANIGELYGSTEGNANVINIDNKIGAVGFMSRIIPTIYPVSLIKVEPSTGEPIRDEKGLCIRCEPGEPGEFVGKIIVTDPLRQFDGYVNKSATKKKIVRDCFQKGDMAFLSGDMLMMDEDGYLYFVDRTGDTFRWRGENVSTMEVEGVISNILGLTDSVVYGVIVPDVEGRAGMAAICDPDNSVDLKHLLNELSKVLPSYAVPQFIRICDKLESTGTFKLKKVDLQKEGFDPSIVKDPLYYLNKKLNAYVKLDAEVYSDICSGRIRI 609          

BLAST of amp dependent ligase vs. nr
Match: gi|1218262963|ref|XP_021710055.1| (long-chain fatty acid transport protein 4 isoform X2 [Aedes aegypti])

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 290/559 (51.88%), Postives = 402/559 (71.91%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPN-VELFSYGSDSSIREGAIKLDREL---VNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQ-LDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VCLI  D++WTF+++ DYSNRVA+ F+   YK G+ V L + N PE++ +WLG SKLG++  LIN NL    LLHS+   + KA I+G  L   +Q I   +P+ +ELF +  + ++++  + +  +L   + ++  E P +  N    +DKLLYIYTSGTTGLPKAAVI HSRF F  A ++ +   G   D +FY PLPLYH+AGG++ +G  ++FGATVVIRKKFS   ++ DC + NC   QYIGE+CRY+L+ P++  +K HKV ++FGNGLRPQIW QF  RF I R+ EFYGATEGN+N++NID+  GA+GF S + P VYP+ +IR +  +G  +R  +GLC LC+P EPG F+GKI+ N+PSR+F GYVD +A+EKKIVR++ K+GD  F SGDLL  DE G ++F DR GDTFRW+GENVST+EVEA +SN    +DT+VYGVEIP +EGRAGMAA+++P  ++D  K  Q +K+ LP+YA P+F+RL+ + +D+TGTFKLKK+ LQ+EGYDPNVI D L FY S KG+Y SL   ++++I + ++R 
Sbjct:   75 KVCLIFEDRKWTFREVNDYSNRVANVFLNNKYKRGEVVGLMLENRPEFVAMWLGLSKLGVIVPLINHNLRKNALLHSVNVANCKALIYGESLRDAVQEIKESLPSSLELFQF--NDAVQQPVLDIAHDLASMLQNASKEQPTANVNKPDHHDKLLYIYTSGTTGLPKAAVITHSRFVFITAAIHMV--AGFRNDDIFYTPLPLYHTAGGMMSIGQALLFGATVVIRKKFSASQYFADCKKNNCTVGQYIGEMCRYILATPDSGTDKAHKVRLIFGNGLRPQIWPQFVERFNIPRVAEFYGATEGNANIVNIDNTVGAIGFVSRIIPQVYPISIIRADPATGEPIRGKNGLCQLCEPNEPGVFIGKILPNNPSRAFLGYVDKSASEKKIVRDIFKKGDAAFLSGDLLVADERGNLFFKDRTGDTFRWKGENVSTSEVEAEVSNASGYRDTVVYGVEIPNMEGRAGMAAVLDPERQVDLTKLAQTLKETLPSYARPMFVRLLTKDMDMTGTFKLKKLDLQKEGYDPNVIEDAL-FYLSPKGSYESLTKEVFDQISRGEIRF 628          

BLAST of amp dependent ligase vs. nr
Match: gi|108884157|gb|EAT48382.1| (AAEL000572-PA [Aedes aegypti])

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 290/559 (51.88%), Postives = 402/559 (71.91%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPN-VELFSYGSDSSIREGAIKLDREL---VNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQ-LDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VCLI  D++WTF+++ DYSNRVA+ F+   YK G+ V L + N PE++ +WLG SKLG++  LIN NL    LLHS+   + KA I+G  L   +Q I   +P+ +ELF +  + ++++  + +  +L   + ++  E P +  N    +DKLLYIYTSGTTGLPKAAVI HSRF F  A ++ +   G   D +FY PLPLYH+AGG++ +G  ++FGATVVIRKKFS   ++ DC + NC   QYIGE+CRY+L+ P++  +K HKV ++FGNGLRPQIW QF  RF I R+ EFYGATEGN+N++NID+  GA+GF S + P VYP+ +IR +  +G  +R  +GLC LC+P EPG F+GKI+ N+PSR+F GYVD +A+EKKIVR++ K+GD  F SGDLL  DE G ++F DR GDTFRW+GENVST+EVEA +SN    +DT+VYGVEIP +EGRAGMAA+++P  ++D  K  Q +K+ LP+YA P+F+RL+ + +D+TGTFKLKK+ LQ+EGYDPNVI D L FY S KG+Y SL   ++++I + ++R 
Sbjct:  170 KVCLIFEDRKWTFREVNDYSNRVANVFLNNKYKRGEVVGLMLENRPEFVAMWLGLSKLGVIVPLINHNLRKNALLHSVNVANCKALIYGESLRDAVQEIKESLPSSLELFQF--NDAVQQPVLDIAHDLASMLQNASKEQPTANVNKPDHHDKLLYIYTSGTTGLPKAAVITHSRFVFITAAIHMV--AGFRNDDIFYTPLPLYHTAGGMMSIGQALLFGATVVIRKKFSASQYFADCKKNNCTVGQYIGEMCRYILATPDSGTDKAHKVRLIFGNGLRPQIWPQFVERFNIPRVAEFYGATEGNANIVNIDNTVGAIGFVSRIIPQVYPISIIRADPATGEPIRGKNGLCQLCEPNEPGVFIGKILPNNPSRAFLGYVDKSASEKKIVRDIFKKGDAAFLSGDLLVADERGNLFFKDRTGDTFRWKGENVSTSEVEAEVSNASGYRDTVVYGVEIPNMEGRAGMAAVLDPERQVDLTKLAQTLKETLPSYARPMFVRLLTKDMDMTGTFKLKKLDLQKEGYDPNVIEDAL-FYLSPKGSYESLTKEVFDQISRGEIRF 723          

BLAST of amp dependent ligase vs. nr
Match: gi|1218262959|ref|XP_021710053.1| (long-chain fatty acid transport protein 4 isoform X1 [Aedes aegypti] >gi|1218262961|ref|XP_021710054.1| long-chain fatty acid transport protein 4 isoform X1 [Aedes aegypti])

HSP 1 Score: 598.586 bits (1542), Expect = 0.000e+0
Identity = 290/559 (51.88%), Postives = 402/559 (71.91%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPN-VELFSYGSDSSIREGAIKLDREL---VNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQ-LDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VCLI  D++WTF+++ DYSNRVA+ F+   YK G+ V L + N PE++ +WLG SKLG++  LIN NL    LLHS+   + KA I+G  L   +Q I   +P+ +ELF +  + ++++  + +  +L   + ++  E P +  N    +DKLLYIYTSGTTGLPKAAVI HSRF F  A ++ +   G   D +FY PLPLYH+AGG++ +G  ++FGATVVIRKKFS   ++ DC + NC   QYIGE+CRY+L+ P++  +K HKV ++FGNGLRPQIW QF  RF I R+ EFYGATEGN+N++NID+  GA+GF S + P VYP+ +IR +  +G  +R  +GLC LC+P EPG F+GKI+ N+PSR+F GYVD +A+EKKIVR++ K+GD  F SGDLL  DE G ++F DR GDTFRW+GENVST+EVEA +SN    +DT+VYGVEIP +EGRAGMAA+++P  ++D  K  Q +K+ LP+YA P+F+RL+ + +D+TGTFKLKK+ LQ+EGYDPNVI D L FY S KG+Y SL   ++++I + ++R 
Sbjct:  165 KVCLIFEDRKWTFREVNDYSNRVANVFLNNKYKRGEVVGLMLENRPEFVAMWLGLSKLGVIVPLINHNLRKNALLHSVNVANCKALIYGESLRDAVQEIKESLPSSLELFQF--NDAVQQPVLDIAHDLASMLQNASKEQPTANVNKPDHHDKLLYIYTSGTTGLPKAAVITHSRFVFITAAIHMV--AGFRNDDIFYTPLPLYHTAGGMMSIGQALLFGATVVIRKKFSASQYFADCKKNNCTVGQYIGEMCRYILATPDSGTDKAHKVRLIFGNGLRPQIWPQFVERFNIPRVAEFYGATEGNANIVNIDNTVGAIGFVSRIIPQVYPISIIRADPATGEPIRGKNGLCQLCEPNEPGVFIGKILPNNPSRAFLGYVDKSASEKKIVRDIFKKGDAAFLSGDLLVADERGNLFFKDRTGDTFRWKGENVSTSEVEAEVSNASGYRDTVVYGVEIPNMEGRAGMAAVLDPERQVDLTKLAQTLKETLPSYARPMFVRLLTKDMDMTGTFKLKKLDLQKEGYDPNVIEDAL-FYLSPKGSYESLTKEVFDQISRGEIRF 718          

BLAST of amp dependent ligase vs. nr
Match: gi|1355996190|gb|PRD36160.1| (Long-chain fatty acid transport protein 1 [Nephila clavipes])

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 289/557 (51.89%), Postives = 396/557 (71.10%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADL---TPNMQSIVHQIPNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            ++C++H DK+WTFQ++EDYSN+VA+ F +  +  GD VA+FM +  E++ +WLG SK+G+VPALIN+NL  +PL  SI +V  KA IFG++L     ++  ++ ++  ++ F  G   S    A  LD  +  +SI   P   K     ND+L Y+YTSGTTG+PKAA+I+HSRF +  AG++Y+N +   +D V Y  LPLYH+AGGI+ +   ++FG +  IR KFS   FW DC +Y    AQYIGE+CRY+L+ PE P+++QHKV++MFGNGLRPQIW QF +RF I  IGE YG+TEGN+NV+NID+  GAVGF S + P +YPV LI+V+  +G  +RD+ GLCI C+PGEPGEFVGKI+ + P R FDGYV+ +AT+KKI+R+   +GD  F SGDLL MDE G++YFVDR GDTFRWRGENVST EVE ++S VL+  D++VYGVE+P  EGRAGMAAI +P  ++D    L  + + LP YA P+F+R+ + L+ TGTFKLKK+ LQ EG++P+ I DPL+FYD  +G +  LD   YN I   ++RL
Sbjct:   29 KICIMHDDKKWTFQEIEDYSNQVANCFAKLGFSYGDDVAVFMESKVEFVAIWLGLSKIGVVPALINSNLRQQPLAFSITSVKCKAVIFGSELIQAVKDVSPLLKELDGLQYFCLGKFESSVFLAKSLDTLIPENSITP-PKHYKG--DMNDRLFYVYTSGTTGMPKAAIIRHSRFMWLGAGIHYMNKIE--KDDVLYTALPLYHTAGGILSLSQTLLFGNSCGIRSKFSASKFWDDCIKYEATVAQYIGELCRYILAQPEKPQDRQHKVKLMFGNGLRPQIWKQFIDRFNIPNIGELYGSTEGNANVINIDNKLGAVGFMSRIIPTIYPVSLIKVDANTGEPIRDNKGLCIRCEPGEPGEFVGKIITSDPIRQFDGYVNQSATKKKIIRDCFTKGDTAFLSGDLLMMDEDGYLYFVDRTGDTFRWRGENVSTMEVEGLISKVLNHADSVVYGVEVPDNEGRAGMAAISDPDHKVDLKILLDELVRVLPPYAVPLFVRICDTLEATGTFKLKKVDLQNEGFNPSTIKDPLYFYDKKQGFFLPLDEETYNSICSGQIRL 580          

BLAST of amp dependent ligase vs. nr
Match: gi|817184749|ref|XP_012283173.1| (long-chain fatty acid transport protein 4 [Orussus abietinus] >gi|817184751|ref|XP_012283183.1| long-chain fatty acid transport protein 4 [Orussus abietinus])

HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0
Identity = 278/554 (50.18%), Postives = 391/554 (70.58%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQI-PNVELFSYGSDSSIREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVNESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +VCL+  D+ WTFQ +ED+SN++A+ F R  ++ G+ VALF+ N PEY+G+WLG SKLG++  LIN NL    L+HSI+  + KA IFG+DL+  ++ I   + P V L+ +G    +  G ++ D  L+ +     P  + N   ++DKLLYIYTSGTTGLPKAAVI +SRF F  +G+++L   G      FY PLPLYH+AGG++ +G  ++ G T VIRKKFS   ++ DC +Y C  AQYIGE+CRY+L+ P  PE+ QH+V ++FGNGLRPQIW +F  RF I ++ EFYGATEGN+N++N+DS  GA+GF S + P VYP+ +I+V+  G  +R+S GLC +C+P EPG F+GKI+ N+PSR+F GYV+  A++ K+V +V  +GD  F SGD+L  DE G+++F DR GDTFRW+GENVST+EVEAV+SN+++ +D +VYGVEI G EG+AGMAAI +P G LD +K    +K  LP YA P+F+R + ++D+TGTFKLKK  LQ EGYDP  + D +++ D  K  Y+ L   +Y++I Q K+R 
Sbjct:  179 KVCLVFEDQEWTFQQVEDFSNKIAYVFRR--FEKGEVVALFLENRPEYVGIWLGLSKLGVITPLINTNLRKSTLVHSIKVANCKALIFGSDLSDAVKDISESLDPGVALYRFGEQPGVDTGRVEQDLSLLLTQAPTTPPVIPNKGSYSDKLLYIYTSGTTGLPKAAVITNSRFMFIASGIHFL--AGFRSSDRFYTPLPLYHTAGGVMSIGQALLHGGTTVIRKKFSASAYFNDCVKYKCTVAQYIGEMCRYILAVPSKPEDTQHQVRVIFGNGLRPQIWKEFVKRFKIPQVAEFYGATEGNANIVNVDSTVGAIGFVSRIIPSVYPISIIKVDSDGVPIRNSKGLCQICEPNEPGVFIGKIIPNNPSRAFLGYVNQEASKSKVVHDVFTKGDSAFISGDILVADELGYLFFKDRTGDTFRWKGENVSTSEVEAVVSNLVNYRDCVVYGVEISGTEGKAGMAAIYDPNGTLDISKLAPQIKDQLPTYARPLFVRTLTKIDLTGTFKLKKKDLQEEGYDPRKLQDKIYYLDP-KTGYQLLTPEIYDQIQQHKIRF 727          

BLAST of amp dependent ligase vs. nr
Match: gi|1233172723|ref|XP_022187860.1| (long-chain fatty acid transport protein 4-like [Nilaparvata lugens])

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 290/555 (52.25%), Postives = 383/555 (69.01%), Query Frame = 0

Query:    1 RVCLIHGDKRWTFQDLEDYSNRVAHYFIRQSYKPGDCVALFMGNCPEYIGLWLGCSKLGMVPALINANLVGRPLLHSIEAVSAKACIFGADLTPNMQSIVHQIPNVELFSYGSDSS-IREGAIKLDRELVNSSIDEVPDSVKNCTHFNDKLLYIYTSGTTGLPKAAVIKHSRFYFYCAGMYYLNDMGSIQDPVFYDPLPLYHSAGGIVGVGLMMVFGATVVIRKKFSVRNFWKDCCEYNCNGAQYIGEICRYLLSAPETPEEKQHKVEIMFGNGLRPQIWTQFTNRFGIRRIGEFYGATEGNSNVLNIDSVPGAVGFSSVLFPFVYPVRLIRVN-ESGNVLRDSSGLCILCQPGEPGEFVGKIVKNHPSRSFDGYVDPAATEKKIVRNVLKEGDMWFRSGDLLTMDEFGWMYFVDRLGDTFRWRGENVSTNEVEAVLSNVLHQQDTIVYGVEIPGVEGRAGMAAIVEPTGRLDFAKFLQGVKKNLPAYATPIFIRLVEQLDITGTFKLKKMSLQREGYDPNVISDPLFFYDSVKGAYRSLDTCLYNEIMQMKLRL 553
            +  ++H    +TF  +E+YSNRVA+YF  Q  K GD VA+FM   PEY+ +WLG SK+G V AL+N N     L HS++  S KA I G +L+ +++ I+  IP+V+++  G D S +      LD  L +SS D +P  +       DK  YIYTSGTTGLPKAAV+ H RF F       + ++   +D V YDPLPLYH+AGG++G+G  ++ G+TVVIR KFS  NFW DC +YNC  AQYIGE+CRYLL+ PE PEEKQHKV ++ GNGLRPQIW QF  RF I R+GEFYGATEGN+N++N  +  GAVG+       VYPV L++ + E+   +R  +GLCI C+ GEPG  +G I K+ PS  F GY D  A+EKKI+R+V K GD +F SGDLL  DE G+ YF DR GDTFRWRGENV+T+EVEAV+SNV+  +D +VYGVEIP VEG+AGMAAIV+   +LD  +   G+K +LP+YA P+F+R++EQ+ +T TFKLKK+ LQ EGYDP+ I+DPL+F D+  G Y +L   L+ EI++ K RL
Sbjct:   93 KAAIVHESSTFTFSQVEEYSNRVANYFKAQGIKRGDAVAIFMEARPEYVCIWLGLSKIGAVSALVNTNQRSNVLAHSLQVASCKAVIVGTELSSHIKEILSSIPDVDIYLTGKDKSKVDLKGKDLDSGLQSSSTDPLPKDIA-LAKMTDKFAYIYTSGTTGLPKAAVMTHCRFMFMVVSTNKVVNI--TEDDVVYDPLPLYHTAGGLLGIGQALLCGSTVVIRSKFSASNFWNDCIKYNCTVAQYIGEMCRYLLALPEKPEEKQHKVRLIVGNGLRPQIWAQFMKRFNIERVGEFYGATEGNANLMNGYNKIGAVGYVPWFAVPVYPVTLVKADPETNEPIRGENGLCIKCETGEPGILIGAIKKSQPSSHFSGYADKKASEKKIIRDVFKRGDEYFNSGDLLVRDECGYYYFKDRTGDTFRWRGENVATSEVEAVISNVVGLRDAVVYGVEIPNVEGKAGMAAIVDEQKQLDIGQLENGIKNSLPSYARPLFLRVIEQVPLTATFKLKKLDLQSEGYDPSKINDPLYFMDAKSGKYVTLTPQLFQEIIEGKQRL 644