BLAST of glutamate synthase vs. L. salmonis genes
Match: EMLSAG00000004194 (supercontig:LSalAtl2s:LSalAtl2s221:469662:476711:-1 gene:EMLSAG00000004194 transcript:EMLSAT00000004194 description:"augustus_masked-LSalAtl2s221-processed-gene-4.2")

HSP 1 Score: 376.326 bits (965), Expect = 8.954e-113
Identity = 197/340 (57.94%), Postives = 240/340 (70.59%), Query Frame = 0

Query:  393 QAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLD--EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            +A +  ++ +Y  Y  A  E  +   +IRG F+ D     P+D  EVE  ++IVKRF TGAMS+GSIS EAH  LA+AMN++GGKSN+GEGGE+ +R+  DI    R SAIKQVASGRFGV++ YL +A E+QIKMAQGAKPGEGG+LPGYKV   IA  R S P                                ARI+VKLVSEVGVG IAAGVAK KA+ +LI+G+DGGTGAS  T +K AGLPWELG+AE  QTLVMNNLRSR+V++ DGQL+TG DV +A +LGA+EFGF+TAPL+  GC MMRKCHLNTCPVGIATQD  LRK F+G PEH++
Sbjct:  768 EATKYENKNAYQKYVDATMETIKKC-SIRGQFEIDFSKGTPVDVSEVELASNIVKRFVTGAMSFGSISAEAHGTLAVAMNKLGGKSNTGEGGENEERYLNDI----RRSAIKQVASGRFGVSASYLAHADELQIKMAQGAKPGEGGELPGYKVTVEIAKTRRSVP--------------------------------ARISVKLVSEVGVGVIAAGVAKGKAEHILISGHDGGTGASSWTGIKGAGLPWELGIAETHQTLVMNNLRSRVVLQTDGQLRTGFDVMVAGILGADEFGFSTAPLIVLGCTMMRKCHLNTCPVGIATQDPILRKKFEGQPEHVV 1070          

HSP 2 Score: 126.716 bits (317), Expect = 6.506e-30
Identity = 66/155 (42.58%), Postives = 80/155 (51.61%), Query Frame = 0

Query:    2 PEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK------------------------------SDSASMDMVVELLTHTG-RSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            P  I  Y+ DL + +  + L +VH RFSTNT P+WE A P R +  NGEINTLRG                               SDS  +D VVE L H G RSLPE MM ++PEAW+    M   +K FY +    MEPWDGPA + FTD R
Sbjct:  211 PCQIWNYFTDLMDPEFESHLLIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNVNLMEAREGVMVSPDFEDLSTLYPIVEPDMSDSGCLDNVVEFLVHVGDRSLPEAMMTLVPEAWQNDDQMPSNKKNFYHWASFYMEPWDGPALLTFTDGR 365          

HSP 3 Score: 98.5969 bits (244), Expect = 3.764e-21
Identity = 57/146 (39.04%), Postives = 82/146 (56.16%), Query Frame = 0

Query:  127 CRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKN-NIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEG 271
            C +L ++NP++S  DLE ++   I  +K+  I+I Y     ++ L D +  I  + E+A++K+   ++ILSDR V     P+ A LA   V+H L   R RS   II+ES E RE HH   L G+GA A+ PYMV EI      EG
Sbjct:  622 CTRLWLKNPILSVNDLEHLKRTSILGWKSRVIDITYLSDDQIS-LSDIVSNICNRAEEAVKKEGVQLLILSDRNVGVNRIPVSAALASGAVHHHLIATRLRSKCAIIVESGEAREVHHLCVLIGFGADAICPYMVLEIAAMLKDEG 766          

BLAST of glutamate synthase vs. SwissProt
Match: gi|1707984|sp|P55037.1|GLTB_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 1; AltName: Full=Fd-GOGAT)

HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0
Identity = 382/603 (63.35%), Postives = 467/603 (77.45%), Query Frame = 0

Query:  127 CRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQ-KDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH 728
            CR + ++ P+++N DL K++ +D + FK+ T++IL+   QG  GL+ ALD +  + ++AI +  N+IILSDR V+ E A IPALLA S ++H L R   R+   +++ES EPRE HHFA L GYG  A+NPY+  E +   + EG +  +D + A  N+ KA  KG++KV +KIGIST+ SYRG+QIFE VG N   +++YF  T+SRI+G  L  I +E   R++ A+     D +  L+VGG+Y+WR++GE HLF+P T+  LQ+AVR  +   Y  YA  +NEQ +   T+RGL  F + + IPL+EVEP   I+KRFKTGAMSYGSIS+EAHE+LAIAMNRIGGKSN+GEGGED +RF   +  GDS+NSAIKQVASGRFGVTS YL+ AKEIQIKMAQGAKPGEGGQLPG KV PWIA  R+STP VGLISPPPHHDIYSIEDLA+LI+DLKNANREARINVKLVSEVGVGTIAAGVAKA ADVVL++GYDGGTGASP TS+KHAGLPWELGLAE  QTLV+NNLRSRIVVE DGQ+KTGRDVAIAALLGAEEFGF+TAPLV+ GCIMMR CHLNTCPVGIATQ+ ELR  F G P H
Sbjct:  581 CRLIELKTPILTNEDLAKLKALDDDEFKSVTLDILFDPNQGEAGLKTALDNLFTEADQAISQGANLIILSDRQVSAEKAAIPALLAVSGLHHHLIRNGSRTKVGLVLESGEPREVHHFAVLLGYGCGAINPYLAFETLDGMIAEGLLVNVDHKTACKNYIKAATKGVIKVASKIGISTIQSYRGAQIFEAVGLNQSVIDEYFCRTSSRIQGSDLGVIAQEAILRHQHAFAPRPGDLHT-LDVGGEYQWRKDGEEHLFSPQTIHLLQRAVREGNYELYKQYAALVNEQNQKFFTLRGLLDFQDRESIPLEEVEPIEAIMKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEGGEDPERFTWTNDQGDSKNSAIKQVASGRFGVTSLYLSQAKEIQIKMAQGAKPGEGGQLPGKKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAELIHDLKNANREARINVKLVSEVGVGTIAAGVAKAHADVVLVSGYDGGTGASPQTSIKHAGLPWELGLAETHQTLVLNNLRSRIVVETDGQMKTGRDVAIAALLGAEEFGFSTAPLVSLGCIMMRACHLNTCPVGIATQNPELRAKFTGDPAH 1182          

HSP 2 Score: 149.443 bits (376), Expect = 1.398e-35
Identity = 68/152 (44.74%), Postives = 91/152 (59.87%), Query Frame = 0

Query:    5 IGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK-------------------------------SDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            +G YY +L + D+ + LALVH RFSTNT P+WE + P+R++  NGEINT+RG                                SDS   D  +ELL   GRSLP  +MMMIPE W  H +M++E+KAFY+Y+ C+MEPWDGPAS+ FT+ +
Sbjct:  232 VGQYYPELHDPDMESALALVHSRFSTNTFPSWERSHPYRYIAHNGEINTMRGNVNWMQARQALFESSLFGEDMAKVQPVINIDGSDSTIFDNALELLYLAGRSLPHAVMMMIPEPWSAHESMSQEKKAFYKYHSCLMEPWDGPASIAFTNGK 383          

BLAST of glutamate synthase vs. SwissProt
Match: gi|81556736|sp|P96218.3|GLTB_MYCTU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain)

HSP 1 Score: 717.227 bits (1850), Expect = 0.000e+0
Identity = 358/620 (57.74%), Postives = 452/620 (72.90%), Query Frame = 0

Query:  123 DDRQCRKLRIQNPVISNADLEKIRNIDI--------ESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAY---PDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDN--LDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHI 729
            D   C ++ +  P++ N +L K+ ++D            +++ I  LY  ++G  GL  AL+ +      AI     IIILSDR  ++E+APIP+LLA + V+H L R R R+   +++ES + RE HH A L G+GA+A+NPY+V E I   +  G I GID   A++N+ KA GKG+LKVM+K+GISTL SY G+Q+F+ VG + Q +++YF        GI L +I  ++  R++ AY   PD +  + L   VGG+Y+WRR GE HLFNP TV KLQ + R      +  Y + +++Q+E + ++RGL +F      P+PLDEVEP ++IVKRF TGAMSYGSIS EAHE LAIAMNR+G +SN GEGGED KRF +D NGD R SAIKQVAS RFGVTSHYLTN  ++QIKMAQGAKPGEGGQLPG+KV PW+A  R+STP VGLISPPPHHDIYSIEDLAQLI+DLKNAN  AR++VKLVSE GVGT+AAGV+KA ADVVLI+G+DGGTGA+PLTS+KHAG PWELGLAE QQTL++N LR RIVV+ DGQLKTGRDV IA LLGAEEFGFATAPLV +GCIMMR CHL+TCPVG+ATQ+  LR+ F G PE +
Sbjct:  552 DQNSCHQIVLPQPILRNHELAKLVSLDPNDKVNGRPHGLRSKVIRCLYRVSEGGAGLAAALEEVRGAAAAAIADGARIIILSDRESDEEMAPIPSLLAVAGVHHHLVRERTRTQVGLVVESGDAREVHHMAALVGFGAAAINPYLVFESIEDMLDRGVIEGIDRTAALNNYIKAAGKGVLKVMSKMGISTLASYTGAQLFQAVGISEQVLDEYFTGLTCPTGGITLDDIAADVAARHRLAYLDRPDERAHREL--EVGGEYQWRREGEYHLFNPETVFKLQHSTRTGQYKIFKEYTRLVDDQSERMASLRGLLKFRTGVRPPVPLDEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGARSNCGEGGEDVKRFDRDPNGDWRRSAIKQVASARFGVTSHYLTNCTDLQIKMAQGAKPGEGGQLPGHKVYPWVAEVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPSARVHVKLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTSMKHAGAPWELGLAETQQTLLLNGLRDRIVVQVDGQLKTGRDVMIATLLGAEEFGFATAPLVVAGCIMMRVCHLDTCPVGVATQNPLLRERFTGKPEFV 1169          

HSP 2 Score: 139.813 bits (351), Expect = 1.482e-32
Identity = 67/149 (44.97%), Postives = 86/149 (57.72%), Query Frame = 0

Query:    8 YYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK---------------------------------SDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            +Y DLQ++ L + L +VH RFSTNT P+W LA PFR +  NGEINT+ G                                  SD+A  D V+ELL   GRSL   ++MMIPEAWE+H +M   R+AFY+Y+  +MEPWDGPAS+ FTD
Sbjct:  209 FYLDLQDERLTSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALIKTDIFGSAADVEKLFPICTPGASDTARFDEVLELLHLGGRSLAHAVLMMIPEAWERHESMDPARRAFYQYHASLMEPWDGPASMTFTD 357          

BLAST of glutamate synthase vs. SwissProt
Match: gi|239938894|sp|P39812.3|GLTA_BACSU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=NADPH-GOGAT)

HSP 1 Score: 715.301 bits (1845), Expect = 0.000e+0
Identity = 356/608 (58.55%), Postives = 448/608 (73.68%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFD-NLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            +R  R++++  PV+SN     ++ I     K++ I++L+ +      LE  L  +  Q EKAI +  +++ILSD+ +N+ L PIP LLA S ++  L R   R+   II+ES E RE HHFA L GYGA A+NPY+     + ++ EG +  I  + AV  + K+I +G++KVM+K+GIST+ SYRG+QIFE VG +   +++YF  TAS++ GI L  I +E  +R+++AY D   D +  L  G D++WR  GE H FNP T+  LQ A R +D   +  Y KA +E  E +  +R LF FD N  P+ L+EVE    IVKRFKTGAMS+GS+S+EAHE LAIAMNR+GGKSNSGEGGED KRF  D NGD R SAIKQ+ASGRFGV SHYL NA E+QIKMAQGAKPGEGGQLPG KV PW+A+ R STP VGLISPPPHHDIYSIEDLAQLI+DLKNANR+ARI+VKLVS+ GVGTIAAGVAKA ADV++I+GYDGGTGASP TS+KH GLPWELGLAEA QTL++N LR R+V+E DG+L TGRDV +AALLGAEEFGFATAPLV  GC+MMR CHL+TCPVG+ATQ+ ELRK F G P+HI+
Sbjct:  551 ERNVRRIKLYTPVLSNEQFYALKTIVHPDLKSQKIDVLFSE-----DLERGLKDMFTQAEKAISQGVSLLILSDKKMNERLTPIPPLLAVSALHQHLIRKGLRTKVSIIVESGEAREVHHFAALIGYGADAINPYLAYATYKQEIDEGRLD-ISYEEAVSKYGKSITEGVVKVMSKMGISTVQSYRGAQIFEAVGISRDVIDRYFSGTASQLGGIDLQTIAEEAQRRHREAYQD---DYSKTLEPGSDFQWRNGGEHHAFNPKTIHTLQWACRRNDYNLFKQYTKAADE--ERIGFLRNLFAFDGNRKPLKLEEVESAESIVKRFKTGAMSFGSLSKEAHEALAIAMNRLGGKSNSGEGGEDPKRFVPDENGDDRRSAIKQIASGRFGVKSHYLVNADELQIKMAQGAKPGEGGQLPGNKVYPWVADVRGSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANRDARISVKLVSKAGVGTIAAGVAKATADVIVISGYDGGTGASPKTSIKHTGLPWELGLAEAHQTLMLNGLRDRVVLETDGKLMTGRDVVMAALLGAEEFGFATAPLVVLGCVMMRACHLDTCPVGVATQNPELRKKFMGDPDHIV 1147          

HSP 2 Score: 139.428 bits (350), Expect = 1.854e-32
Identity = 65/156 (41.67%), Postives = 85/156 (54.49%), Query Frame = 0

Query:    2 PEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK-------------------------------SDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDRQ 126
            PE +  +Y DLQ++  V+  ALVH RFSTNT PTWE A P R++  NGEINTLRG                                SDS+ +D   E     GR      MM+IPE W ++  M+KE++AFYEY+  +MEPWDGP ++ FTD +Q
Sbjct:  204 PEQVDAFYSDLQDEAFVSAFALVHSRFSTNTFPTWERAHPNRYLVHNGEINTLRGNINWMRAREQQFVSESFGEDLNKILPILNADGSDSSILDNAFEFFVMAGRKPAHTAMMLIPEPWTENTHMSKEKRAFYEYHSSLMEPWDGPTAISFTDGKQ 359          

BLAST of glutamate synthase vs. SwissProt
Match: gi|46395959|sp|Q9C102.1|GLT1_SCHPO (RecName: Full=Putative glutamate synthase [NADPH]; AltName: Full=NADPH-GOGAT)

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 334/610 (54.75%), Postives = 448/610 (73.44%), Query Frame = 0

Query:  126 QCRKLRIQNPVISNADLEKIRNID--IESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFIT-GIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGL--FQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            QCR+LR+  P+++  +   ++N+D     +K  +I+I + K++G+ G   A++ I  + + A+ +    I+LSDR VN E  P+ ++ AC  V+H L + + RS   ++ ES + RE HH  TL GYGA AV PY+  E +   V++  +  GI E+ A+ NF  AI  GILKVM+K+GISTL SY+G+QIFE +G +++ + K F  TASRI G+    I  +    +++ YP +Q  ++L +   GD+ +R  GE+H+ +P  +A LQ AVR  ++A+Y  +++   EQ     T+RG+  F FD+   IP+++VEPWT+IV+RF TGAMSYGSIS E+H +LAIAMNR+GGKSN+GEGGED  R Q+  NGD+  SAIKQ+ASGRFGVTS YL++A E+QIKMAQGAKPGEGG+LPG KV   IA  R+ST  VGLISPPPHHDIYSIEDL QLIYD+K+AN  AR++VKLVSEVGVG +A+GVAKAKAD +L++G+DGGTGAS  T +K+AGLPWELG+AE  QTLV+N+LR R+V++ DGQ++TGRDVAIA LLGAEE+GFAT PL+A GCIMMRKCHLNTCPVGIATQD ELRK F+G PEH++
Sbjct:  618 QCRRLRMPTPILTVEEFNALKNVDRIYPDWKVASIDITFFKSEGVAGYAAAIERICSEADTAVNEGYKAIVLSDRNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDAREVHHMCTLLGYGADAVCPYLAMEALTKLVRQNAMKPGITEETAIKNFKHAINGGILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHERGYPTDQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSRTHYEQTRRC-TLRGMLDFDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGGKSNTGEGGEDPARSQRLANGDTMRSAIKQIASGRFGVTSWYLSDADELQIKMAQGAKPGEGGELPGNKVSESIAKTRHSTAGVGLISPPPHHDIYSIEDLKQLIYDMKSANPRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAETHQTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALGCIMMRKCHLNTCPVGIATQDPELRKKFEGQPEHVV 1226          

HSP 2 Score: 133.65 bits (335), Expect = 1.571e-30
Identity = 68/158 (43.04%), Postives = 85/158 (53.80%), Query Frame = 0

Query:    2 PEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK-------------------------------SDSASMDMVVELLTHTG-RSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDRQC 127
            P  +  Y+ DL   + V+  ALVH RFSTNT P+W+ AQP R    NGEINTLRG                                SDSA+ D V+ELL  +G  SLPE +M++IPEAW+    ++ E+ AFYE+  C MEPWDGPA   F D R C
Sbjct:  257 PVQVYNYFLDLNNAEYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMKSSRFGEEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKAAFYEWAACQMEPWDGPALFTFADGRYC 414          

BLAST of glutamate synthase vs. SwissProt
Match: gi|300680981|sp|Q9LV03.2|GLUT1_ARATH (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor)

HSP 1 Score: 644.425 bits (1661), Expect = 0.000e+0
Identity = 340/637 (53.38%), Postives = 446/637 (70.02%), Query Frame = 0

Query:  110 MEPWDGP-ASVPFTDDRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYM-VNEIIRTQVKEGFITGID------EQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQID----KNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLD-PIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRN---SAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            ME   GP   +  T + QC +L ++ P++   ++E I+ ++   ++ + ++I Y K +G  GLE+ LD I  +  +AI++   +++LSDR  +     + +L+A   V+H L +   R+   +++ESAEPRE HHF TL G+GA A+ PY+ V  + R QV +G I          ++  V  + KA   G++KV+ K+GISTL SY+G+QIFE +G +S+ ++K F  T SR+EG     + ++  Q ++ A+P         +   L   G+Y WR+NGE HL +P  +AKLQ+A R +  A+Y  Y+K INE  +    +RGL +F + D  IPLDEVEP ++IVKRF TGAMSYGSIS EAH  LA+AMN++GGKSN+GEGGE   R +   +G SRN   S+IKQ+ASGRFGV+S+YLTNA E+QIKMAQGAKPGEGG+LPG+KV+  IA  RNST  VGLISPPPHHDIYSIEDLAQLI+DLKNAN  ARI+VKLVSE GVG IA+GV K  AD VLIAG+DGGTGAS  T +K+AGLPWELGLAE  QTLV N+LR R V++ DGQLKTGRDVA+AALLGAEEFGF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEH++
Sbjct:  665 MECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQV-DGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQ-SNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADG-SRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVI 1298          

HSP 2 Score: 137.887 bits (346), Expect = 6.772e-32
Identity = 69/152 (45.39%), Postives = 82/152 (53.95%), Query Frame = 0

Query:    8 YYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRG----------------------------------KSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            YY DL  +   + +ALVH RFSTNT P+W+ AQP R +  NGEINTLRG                                   SDS + D V+ELL   GRSLPE +MMMIPEAW+    +   RK FYEY   +MEPWDGPA + FTD R
Sbjct:  313 YYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGR 464          

BLAST of glutamate synthase vs. SwissProt
Match: gi|417073|sp|Q03460.1|GLSN_MEDSA (RecName: Full=Glutamate synthase [NADH], amyloplastic; AltName: Full=NADH-GOGAT; Flags: Precursor)

HSP 1 Score: 639.032 bits (1647), Expect = 0.000e+0
Identity = 339/627 (54.07%), Postives = 435/627 (69.38%), Query Frame = 0

Query:  122 TDDRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEII-RTQVK-------EGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQID----KNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINE--QAENLMTIRGLFQF-DNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRN---SAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            T + QC +L ++ P++S  ++E I+ ++   ++++ I+I Y K +G  GLE+ALD I  +   AI +    ++LSDR  +++   + +LLA   V+  L +  +R+   +++ESAEPRE HHF TL G+GA A+ PY+  E I R QV         G     DE   V  + KA   G++KV+ K+GISTL SY+G+QIFE +G +S+ +EK F  T SR+EG     + ++    ++ A+P         + + L   GDY WR+ GE HL +P  +AKLQ+A R +   +Y  Y+K I+E  +A NL   RGL +F D    +P+ EVEP ++IVKRF TGAMSYGSIS EAH  LA AMN IGGKSN+GEGGE   R +   +G SRN   SAIKQVASGRFGV+S+YLTNA E+QIKMAQGAKPGEGG+LPG+KV+  IA  RNST  VGLISPPPHHDIYSIEDLAQLI+DLKNAN  ARI+VKLVSE GVG IA+GV K  A+ VLI+G+DGGTGAS  T +K AGLPWELGLAE  QTLV N+LR R  ++ DGQLKTGRDVAIAALLGAEE+GF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEH++
Sbjct:  660 TTEEQCHRLSLKGPLLSTKEMEAIKKMNYRGWRSKVIDITYSKERGTKGLEEALDRICTEAHNAISEGYTTLVLSDRAFSKKHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDE--LVKKYFKASTYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELAFPSRIFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTIHELNKACNL---RGLLKFKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMNTIGGKSNTGEGGEQPSRMEPLADG-SRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGLAETHQTLVANDLRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVI 1280          

HSP 2 Score: 143.28 bits (360), Expect = 1.519e-33
Identity = 69/150 (46.00%), Postives = 84/150 (56.00%), Query Frame = 0

Query:    8 YYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRG----------------------------------KSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            YY DL  +   + +AL+H RFSTNT P+W+ AQPFR +  NGEINTLRG                                   SDS   D V+E L H+G+SLPE +MMMIPEAW+    M  +RKAFYEY   +MEPWDGPA + FTD
Sbjct:  295 YYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREGLLKCKELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLLHSGKSLPEAVMMMIPEAWQNDKNMDPQRKAFYEYYSALMEPWDGPALISFTD 444          

BLAST of glutamate synthase vs. SwissProt
Match: gi|300680979|sp|Q0DG35.2|GLT2_ORYSJ (RecName: Full=Glutamate synthase 2 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 2; Short=NADH-GOGAT 2; Flags: Precursor)

HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0
Identity = 336/636 (52.83%), Postives = 442/636 (69.50%), Query Frame = 0

Query:  110 MEPWDGP-ASVPFTDDRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEII-RTQVKEGFITGID-----EQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQI-----DKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNL-DPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRF--QKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            ME   GP   +  T +RQC +L +++P+++  ++E I+ ++   ++++ ++I YPK  G  GL+  LD I  Q  +AI +   I++LSDRG + E   + +LLA   V+  L    +R+   +++ESAEPRE HHF+TL G+GA A+ PY+  E I R Q+ +G I   D     ++  ++ +  A   G++KV+ K+GISTL SY+G+QIFE +G  S+ V K F  T SR+EG     + ++  + ++ A+P   +     D N  L   GDY WR+NGE HL +P ++AKLQ+A R++ + +Y  Y++ I E      T+RG+ +F  + + I LDEVEP  +IVKRF TGAMSYGSIS EAH +LA AMN +GGKSN+GEGGE   R     D + + R SAIKQVASGRFGV+ +YLTNA E+QIKMAQGAKPGEGG+LPG+KV+  IA  RNST  VGLISPPPHHDIYSIEDLAQLI+DLKNAN  ARI+VKLVSE GVG +A+GV K  AD VLI+G+DGGTGAS  T +K+AGLPWELGLAE  QTLV N LR R V++ DGQ+KTGRDVA+A LLGAEEFGF+TAPL+  GCIMMRKCH NTCP GIATQD  LR  F G PEH++
Sbjct:  653 MECMIGPEGDLSETTERQCHRLTLKSPLLNTNEMEAIKKMNYRGWRSKVLDITYPKKNGRMGLKQTLDKICAQAREAIHEGYTILVLSDRGFSSERVAVSSLLAVGAVHQHLVSHLERTRIGLLVESAEPREVHHFSTLIGFGADAICPYLAIEAIWRLQI-DGRIPPNDGKPYTQEQLIEKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLASEVVSKCFEGTPSRVEGATFEMLAQDALRLHEIAFPSRTLPPGSADAN-ALPNPGDYHWRKNGEVHLNDPFSIAKLQEAARINSREAYKEYSRRIYE-LNKACTLRGMLKFREIPNQISLDEVEPAKEIVKRFCTGAMSYGSISLEAHTSLAEAMNTLGGKSNTGEGGEQPCRMVPLPDGSKNPRISAIKQVASGRFGVSIYYLTNAVEVQIKMAQGAKPGEGGELPGHKVIGDIAVTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGIVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAVLQTDGQMKTGRDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPAGIATQDPVLRAKFAGKPEHVI 1285          

HSP 2 Score: 137.887 bits (346), Expect = 6.030e-32
Identity = 67/154 (43.51%), Postives = 86/154 (55.84%), Query Frame = 0

Query:    6 GPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRG----------------------------------KSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            G ++ DL ++   + +AL+H RFSTNT P+W+ AQP R +  NGEINTLRG                                   SDS ++D V+ELL  +GRS PE +MMMIPEAW+    M  ERKA YE+   +MEPWDGPA + FTD R
Sbjct:  301 GYFFADLGDESFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNKNWMKAREGLLKCEGLGLTRDEMLKLLPIVDATSSDSGAIDNVLELLIQSGRSAPEAVMMMIPEAWQNDVNMDPERKALYEFFSALMEPWDGPALISFTDGR 454          

BLAST of glutamate synthase vs. SwissProt
Match: gi|114152810|sp|Q12680.2|GLT1_YEAST (RecName: Full=Glutamate synthase [NADH]; AltName: Full=NADH-GOGAT; Flags: Precursor)

HSP 1 Score: 627.861 bits (1618), Expect = 0.000e+0
Identity = 318/617 (51.54%), Postives = 429/617 (69.53%), Query Frame = 0

Query:  126 QCRKLRIQNPVISNADLEKIRNIDIE--SFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGID------EQGAV-DNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQ-IDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQ--FDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            QC +L +++P++   + + ++NI+    S+    I+I + K++GL G  D +D I     +AI+    I+I++DR +      I +L+A S ++H L R ++RS   +I+E+ E RE HHF  L GYG   V PY+  E +    +EG +  ++      E+G + +N+  AI  GILKVM+K+GISTL SY+G+QIFE +G ++  V+  F  T+SRI G+    + ++    +++ YP  Q I K++ L   G+Y +R  G +H+  PT +A LQ  VR  +  S+ +Y K   E   +  T+RGL +  F+N   IPL++VEPWT+I +RF +GAMSYGSIS EAH  LAIAMNR+G KSN GEGGED +R     NGD+  SAIKQVAS RFGVTS+YL++A EIQIK+AQGAKPGEGG+LP +KV   IA  R+STP VGLISPPPHHDIYSIEDL QLIYDLK AN  A I+VKLVSEVGVG +A+GVAKAKAD +L++G+DGGTGA+  TS+K+AGLPWELGLAE  QTLV+N+LR  +VV+ DGQL+TG D+A+A LLGAE F  AT PL+A GC+M+R+CHLN+C VGIATQD  LR  FKG PEH++
Sbjct:  604 QCDRLLLKSPILHWNEFQALKNIEAAYPSWSVAEIDITFDKSEGLLGYTDTIDKITKLASEAIDDGKKILIITDRKMGANRVSISSLIAISCIHHHLIRNKQRSQVALILETGEAREIHHFCVLLGYGCDGVYPYLAMETLVRMNREGLLRNVNNDNDTLEEGQILENYKHAIDAGILKVMSKMGISTLASYKGAQIFEALGLDNSIVDLCFTGTSSRIRGVTFEYLAQDAFSLHERGYPSRQTISKSVNLPESGEYHFRDGGYKHVNEPTAIASLQDTVRNKNDVSWQLYVKKEMEAIRDC-TLRGLLELDFENSVSIPLEQVEPWTEIARRFASGAMSYGSISMEAHSTLAIAMNRLGAKSNCGEGGEDAERSAVQENGDTMRSAIKQVASARFGVTSYYLSDADEIQIKIAQGAKPGEGGELPAHKVSKDIAKTRHSTPNVGLISPPPHHDIYSIEDLKQLIYDLKCANPRAGISVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGAARWTSVKYAGLPWELGLAETHQTLVLNDLRRNVVVQTDGQLRTGFDIAVAVLLGAESFTLATVPLIAMGCVMLRRCHLNSCAVGIATQDPYLRSKFKGQPEHVI 1219          

HSP 2 Score: 134.42 bits (337), Expect = 7.691e-31
Identity = 71/165 (43.03%), Postives = 91/165 (55.15%), Query Frame = 0

Query:    2 PEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK-------------------------------SDSASMDMVVELLTHTGR-SLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDRQCRKLRIQN 134
            P  +  YY DL      + +ALVH RFSTNT P+W+ AQP R +  NGEINTLRG                                SDSA++D V+ELLT  G  SLPE +MMM+PEA+  H  M  + KA+Y++  C+MEPWDGPA + FTD R C  +  +N
Sbjct:  245 PAQVYNYYPDLTNAHFKSHMALVHSRFSTNTFPSWDRAQPLRWLAHNGEINTLRGNKNWMRSREGVMNSATFKDELDKLYPIIEEGGSDSAALDNVLELLTINGTLSLPEAVMMMVPEAY--HKDMDSDLKAWYDWAACLMEPWDGPALLTFTDGRYCGAILDRN 407          

BLAST of glutamate synthase vs. SwissProt
Match: gi|122222486|sp|Q0JKD0.1|GLT1_ORYSJ (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor)

HSP 1 Score: 620.542 bits (1599), Expect = 0.000e+0
Identity = 329/623 (52.81%), Postives = 436/623 (69.98%), Query Frame = 0

Query:  122 TDDRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFI------TGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPD-----NQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQF-DNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDING--DSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            T ++QC +L ++ P++S  ++E I+ ++   ++++ ++I YPK  G  GLE+ LD I  +   AI+K   +++LSDRG + +   + +LLA   V+  L    +R+   +++ESAEPRE HHF TL G+GA AV PY+  E I     +G I          ++  V  +  A   G++KV+ K+GISTL SY+G+QIFE +G +S+ + K F  T SRIEG     + ++  + ++ A+P         D    L   GDY WR+NGE HL +P  +AKLQ+A R++ +A+Y  Y++ I E  +    +RG+ +F D  D I +DEVEP ++IVKRF TGAMSYGSIS EAH  LA+AMN++GGKSN+GEGGE   R +   NG  + + SAIKQVASGRFGV+S+YLTNA E+QIKMAQGAKPGEGG+LPG+KV+  IA  R+ST  VGLISPPPHHDIYSIEDLAQLI+DLKN+N  ARI+VKLVSE GVG +A+GV K  AD VLI+G+DGGTGAS  T +K+AGLPWELGLAE  QTLV N LR R +++ DGQLKTG+DVA+A LLGAEEFGF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEH++
Sbjct:  659 TTEKQCNRLALEGPLVSIDEMEAIKKMNYRGWRSKVLDITYPKKSGRKGLEETLDRICTEARGAIKKGYTVLVLSDRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVHHFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFPSRAPPPGSADAK-ALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQELNKTC-NLRGMLKFKDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKLGGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAVTRHSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPRARISVKLVSEAGVGVVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAILQTDGQLKTGKDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPVGIATQDPVLREKFAGEPEHVI 1279          

HSP 2 Score: 138.658 bits (348), Expect = 4.282e-32
Identity = 69/154 (44.81%), Postives = 85/154 (55.19%), Query Frame = 0

Query:    6 GPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRG----------------------------------KSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            G YY DL  ++  + +ALVH RFSTNT P+W+ AQP R +  NGEINTL+G                                   SDS + D V+ELL   GRSLPE +MMMIPEAW+    M  E+KA YE+   +MEPWDGPA + FTD R
Sbjct:  294 GYYYADLGHENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKLGLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGGRSLPEAVMMMIPEAWQNDVNMEPEKKALYEFLSALMEPWDGPALISFTDGR 447          

BLAST of glutamate synthase vs. SwissProt
Match: gi|729601|sp|Q05755.1|GLTB_AZOBR (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain; AltName: Full=NADPH-GOGAT; Flags: Precursor)

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 310/644 (48.14%), Postives = 419/644 (65.06%), Query Frame = 0

Query:  123 DDRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLD-PIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHI--LTTWCIKENGKKLRGYYILPIIFQNLLGAYALLH 763
            D+ Q R L++++PV++ A+   +R  D     A  I+  +P   G   L DAL  I  + E A+      +IL+D  +    A IPA+LA   V+  L R   R++  + + +AE  + H+FA L G GA+ VN Y+  E I  + + G    +  +  + N+ KAI  G+LK+M+K+GIS + SYRG   FE +G +   V ++FP   SRI GIGL  I+K++ +++  AY +  +     L VGG YR+R++G+RH +    +  LQQAV      ++  Y++ +N++    M +R L +  +   P+P+DEVE  T I KRF T  MS G++S EAH  L +AMNRIG KS+SGEGGED  RF+ D NGD+ NSAIKQVASGRFGVT+ YL   +E++IK+AQGAKPGEGGQLPG+KV   IA  R+STP V LISPPPHHDIYSIEDLAQLIYDLK  N +A++ VKLVS  G+GTIAAGVAKA AD++LI+G  GGTGASP TS+K AGLPWE+GL+E  Q L +N LR R+ +  DG LKTGRD+ IAA+LGAEEFG  TA L+A GCIM+R+CH NTCPVG+  QD +LR+ F GTPE +  L T+  +E  + L G     +    ++G   LLH
Sbjct:  572 DETQTRLLQLESPVLTTAEFRAMR--DYMGDTAAEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHVILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAEGLDTHYFAVLIGVGATTVNAYLAQEAIAERHRRGLFGSMPLEKGMANYKKAIDDGLLKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGIGLNGIQKKVLEQHATAYNEEVV----ALPVGGFYRFRKSGDRHGWEGGVIHTLQQAVTNDSYTTFKKYSEQVNKRPP--MQLRDLLELRSTKAPVPVDEVESITAIRKRFITPGMSMGALSPEAHGTLNVAMNRIGAKSDSGEGGEDPARFRPDKNGDNWNSAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPGFKVTEMIARLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRSGIGTIAAGVAKANADIILISGNSGGTGASPQTSIKFAGLPWEMGLSEVHQVLTLNRLRHRVRLRTDGGLKTGRDIVIAAMLGAEEFGIGTASLIAMGCIMVRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREILAGLGFRSL--NEVIGRTDLLH 1205          

HSP 2 Score: 119.013 bits (297), Expect = 4.648e-26
Identity = 57/156 (36.54%), Postives = 80/156 (51.28%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGK-------------------------------SDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTDDR 125
            + E +  +Y DL ++   +  A+ HQR+STNT PTW LAQPFR +  NGEINT++G                                SDS S+D V E++   GR+ P + MM++P+A     T     KA  +Y   +MEPWDGPA++  TD R
Sbjct:  220 LAEQLTTFYPDLLDERFESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNWMKAHETRMEHPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRAGRTAPMVKMMLVPQALTSSQTTPDNHKALIQYCNSVMEPWDGPAALAMTDGR 375          

BLAST of glutamate synthase vs. nr
Match: gi|1057039706|ref|WP_068448652.1| (glutamate synthase large subunit [Polaribacter atrinae] >gi|1026345349|gb|OAD45693.1| glutamate synthase subunit alpha [Polaribacter atrinae])

HSP 1 Score: 1215.29 bits (3143), Expect = 0.000e+0
Identity = 585/610 (95.90%), Postives = 602/610 (98.69%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIRNIDIESFKA+TIEILYPKAQGLNGLEDALDAIV+QVEKAIE KNNIIILSDRGVNQE APIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITG+DEQ AVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAK INEQ+E+LMTIRGLFQFDNLDPIPL+EVEPWT+IVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYL++AKEIQIKMAQGAKPGEGGQLPG KVLPWIA+ARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANR+ARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+N+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAKTIEILYPKAQGLNGLEDALDAIVIQVEKAIENKNNIIILSDRGVNQEFAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKTINEQSESLMTIRGLFQFDNLDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLSSAKEIQIKMAQGAKPGEGGQLPGSKVLPWIASARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANRDARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 252.677 bits (644), Expect = 7.626e-67
Identity = 120/154 (77.92%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+KERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1298075507|gb|AUC22189.1| (glutamate synthase large subunit [Polaribacter sejongensis])

HSP 1 Score: 1214.52 bits (3141), Expect = 0.000e+0
Identity = 585/610 (95.90%), Postives = 602/610 (98.69%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            +RQCRKLRIQNPVISNADLEKIRNIDIESFKA+TIEILYPKAQGLNGLEDALDAIV+QVEKAIE+KNNIIILSDRGVNQE APIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITGIDEQ AVDNFN+AIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYE+EKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAK INEQ+E+LMTIRGLFQFDNLDPIPL+EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYL++AKEIQIKMAQGAKPGEGGQLPG KVLPWIA ARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANR+ARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+N+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 NRQCRKLRIQNPVISNADLEKIRNIDIESFKAKTIEILYPKAQGLNGLEDALDAIVIQVEKAIEEKNNIIILSDRGVNQEFAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGIDEQKAVDNFNQAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEVEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKTINEQSESLMTIRGLFQFDNLDPIPLEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLSSAKEIQIKMAQGAKPGEGGQLPGSKVLPWIAAARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANRDARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 249.98 bits (637), Expect = 6.432e-66
Identity = 119/154 (77.27%), Postives = 120/154 (77.92%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYY DLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+KERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYLDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1306718328|ref|WP_100946248.1| (glutamate synthase large subunit [Polaribacter sp. ALD11] >gi|1304118713|gb|AUC85256.1| glutamate synthase large subunit [Polaribacter sp. ALD11])

HSP 1 Score: 1213.36 bits (3138), Expect = 0.000e+0
Identity = 584/607 (96.21%), Postives = 598/607 (98.52%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHIL 730
            +RQCRKLRIQNPVISNADLEKIRNIDIESFK +TIEILYPKAQGLNGLEDALDAI++QVEKAIE KNNIIILSDRGVNQE APIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITG+DEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGF+SQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTV+KLQQAVRLSDQ SYDVYAKAINEQAE+LMTIRGLF+FDNLDPIPL+EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDR RFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPG KV PWIA  RNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMN+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++
Sbjct:  549 ERQCRKLRIQNPVISNADLEKIRNIDIESFKTKTIEILYPKAQGLNGLEDALDAIIIQVEKAIEDKNNIIILSDRGVNQEFAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGMDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFSSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVSKLQQAVRLSDQGSYDVYAKAINEQAESLMTIRGLFEFDNLDPIPLEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGEKVYPWIAAVRNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNSLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVI 1155          

HSP 2 Score: 251.906 bits (642), Expect = 1.446e-66
Identity = 119/154 (77.27%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYY+DLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+KERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYRDLQEVDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGLQMEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1223861950|ref|WP_091894786.1| (glutamate synthase large subunit [Polaribacter sp. KT25b] >gi|1085841385|emb|SDS27235.1| glutamate synthase (NADH) large subunit [Polaribacter sp. KT25b])

HSP 1 Score: 1208.74 bits (3126), Expect = 0.000e+0
Identity = 580/610 (95.08%), Postives = 602/610 (98.69%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIR+IDIESFKAETI+ILYPKA+GLNGLEDALDAIV+QVEKA+E+KNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRS+FDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITG+DEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPD  ID+NL LN+GGDYRWRRN ERHLFNPTTVAKLQQAVRLSDQASYDVY+KAINEQ+E+LMTIRGLFQFDNLDPIP++EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNA+EIQIKMAQGAKPGEGGQLPG KVLPWIA+ARNSTPFVGLISPPPHHDIYSIEDLAQLI+DLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+NNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRSIDIESFKAETIQILYPKAKGLNGLEDALDAIVLQVEKALEQKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSHFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDKHIDQNLALNIGGDYRWRRNAERHLFNPTTVAKLQQAVRLSDQASYDVYSKAINEQSESLMTIRGLFQFDNLDPIPIEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAQEIQIKMAQGAKPGEGGQLPGAKVLPWIASARNSTPFVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 248.825 bits (634), Expect = 1.832e-65
Identity = 118/154 (76.62%), Postives = 119/154 (77.27%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+  RKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSASRKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1200077767|ref|WP_087520777.1| (glutamate synthase large subunit [Polaribacter sp. SA4-12] >gi|1199785304|gb|ARV15140.1| glutamate synthase large subunit [Polaribacter sp. SA4-12])

HSP 1 Score: 1207.97 bits (3124), Expect = 0.000e+0
Identity = 578/610 (94.75%), Postives = 602/610 (98.69%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETI+ILYPKA+GLNGLEDALD I+V++EKA+E+K NIIILSDRGVN+ELAPIPALLACSFVNHQLNRLRKRS+FDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITG+DEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYE+EKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTV+KLQQAVRLSDQ SYDVYAKAINEQ+E+LMTIRGLFQFDNLDPIPL+EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYL++AKEIQIKMAQGAKPGEGGQLPG KVLPWIA ARNSTPFVGLISPPPHHDIYSIEDLAQLI+DLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+N+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIQILYPKAKGLNGLEDALDDIIVKIEKALERKTNIIILSDRGVNKELAPIPALLACSFVNHQLNRLRKRSHFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEVEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVSKLQQAVRLSDQGSYDVYAKAINEQSESLMTIRGLFQFDNLDPIPLEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLSSAKEIQIKMAQGAKPGEGGQLPGEKVLPWIAEARNSTPFVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 254.988 bits (650), Expect = 1.545e-67
Identity = 121/154 (78.57%), Postives = 122/154 (79.22%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATM+KERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMSKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1200127484|ref|WP_087563758.1| (glutamate synthase large subunit [Polaribacter sp. SA4-10] >gi|1199776556|gb|ARV06395.1| glutamate synthase large subunit [Polaribacter sp. SA4-10])

HSP 1 Score: 1206.82 bits (3121), Expect = 0.000e+0
Identity = 578/610 (94.75%), Postives = 600/610 (98.36%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIRNI+IESFKAETI+ILYPKA+GLNGLEDALDAIV+Q+EKA+E+K NIIILSDRGVN+ELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITG+DEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYE+EKEIDQRYKQAYP+NQIDKNL LNVGGDYRWRRNGERHLFNPTTV+KLQQAVRLSDQ SYDVYAKAINEQ+E+LMTIRGLFQFDNLDPIPL+EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDR+RFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPG KV PWIA  RNSTPFVGLISPPPHHDIYSIEDLAQLI+DLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMN+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRNINIESFKAETIQILYPKAKGLNGLEDALDAIVIQIEKALERKTNIIILSDRGVNKELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEVEKEIDQRYKQAYPENQIDKNLALNVGGDYRWRRNGERHLFNPTTVSKLQQAVRLSDQGSYDVYAKAINEQSESLMTIRGLFQFDNLDPIPLEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRRRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGEKVYPWIAAVRNSTPFVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 252.677 bits (644), Expect = 9.181e-67
Identity = 120/154 (77.92%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATM+KERKAFYEYN CIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMSKERKAFYEYNACIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|738936897|ref|WP_036822860.1| (glutamate synthase large subunit [Polaribacter sp. Hel1_85] >gi|697011202|gb|KGL64384.1| glutamate synthase (NADPH) large chain [Polaribacter sp. Hel1_85])

HSP 1 Score: 1206.82 bits (3121), Expect = 0.000e+0
Identity = 578/610 (94.75%), Postives = 602/610 (98.69%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALD I++Q+EKA+E+K NIIILSDRGVN+ELAPIPALLACSFVNHQLNRLRKRS+FDIIIESAEPREPHHFATLFGYGASA+NPYMVNEIIRTQVKEGFITG+DEQ AVDNFN+AIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTV+KLQQAVRLSDQ SYDVYAK INEQ+E+LMTIRGLFQFDNLDPIPL+EVEPWT+IVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYL++AKEIQIKMAQGAKPGEGGQLPG KVLPWIA+ARNSTPFVGLISPPPHHDIYSIEDLAQLI+DLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+N+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDDIIIQIEKALERKTNIIILSDRGVNKELAPIPALLACSFVNHQLNRLRKRSHFDIIIESAEPREPHHFATLFGYGASAINPYMVNEIIRTQVKEGFITGMDEQKAVDNFNQAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVSKLQQAVRLSDQKSYDVYAKTINEQSESLMTIRGLFQFDNLDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLSSAKEIQIKMAQGAKPGEGGQLPGEKVLPWIASARNSTPFVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 254.218 bits (648), Expect = 2.878e-67
Identity = 121/154 (78.57%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TMTKERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHETMTKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1353612734|ref|WP_105050271.1| (glutamate synthase large subunit [Polaribacter butkevichii] >gi|1351023552|gb|PQJ69340.1| glutamate synthase large subunit [Polaribacter butkevichii])

HSP 1 Score: 1206.05 bits (3119), Expect = 0.000e+0
Identity = 582/610 (95.41%), Postives = 599/610 (98.20%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            DRQCRKLRIQNPVISNADLEKIRNIDIESFKA TI+ILYPKAQGLNGLEDALD IV+QVEKA+E+KNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRS+FDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIR QVKEGFITG+DEQ AVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNS FVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRN ERHLFNPTTVAKLQQAVRLSDQ SY+VYAKAINEQ+ENLMTIRGLFQFDNLDPIPL+EVEPWT+IVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNA+EIQIKMAQGAKPGEGGQLPG KVLPWIA ARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLV+N+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAATIQILYPKAQGLNGLEDALDNIVIQVEKALEQKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSHFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSPFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNSERHLFNPTTVAKLQQAVRLSDQGSYNVYAKAINEQSENLMTIRGLFQFDNLDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAREIQIKMAQGAKPGEGGQLPGAKVLPWIAAARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNDLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 250.366 bits (638), Expect = 5.394e-66
Identity = 119/154 (77.27%), Postives = 120/154 (77.92%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQ  DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+KERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQAIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSKERKAFYEYNGCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|1209309979|ref|WP_088354252.1| (glutamate synthase large subunit [Polaribacter haliotis])

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 578/610 (94.75%), Postives = 596/610 (97.70%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            +RQCRKL I+NPVISNADLEKIR+IDIESFKA TI ILYPKAQGLNGLEDALD I++QVEKAI+ KNNIIILSDRGVNQE APIPALLACSFVNHQLNRLRKRS+FDIIIESAEPREPHHFATLFGYGASA+NPYMVNEIIR QVKEGFI  +DEQ AVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYP+NQIDKNL LN+GGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAK INEQAE+LMTIRGLFQFDNLDPIPLDEVEPWT+IVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNA+EIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGL+EAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 ERQCRKLNIKNPVISNADLEKIRSIDIESFKATTINILYPKAQGLNGLEDALDNIIIQVEKAIQNKNNIIILSDRGVNQEFAPIPALLACSFVNHQLNRLRKRSFFDIIIESAEPREPHHFATLFGYGASAINPYMVNEIIRAQVKEGFIVDMDEQQAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPNNQIDKNLSLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKTINEQAESLMTIRGLFQFDNLDPIPLDEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAREIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLSEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 253.062 bits (645), Expect = 5.311e-67
Identity = 120/154 (77.92%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKH TM+KERKAFYEYN CIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGSQIEELFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHKTMSKERKAFYEYNSCIMEPWDGPASVPFTD 352          

BLAST of glutamate synthase vs. nr
Match: gi|928948243|ref|WP_053972969.1| (glutamate synthase large subunit [Polaribacter dokdonensis] >gi|926665634|gb|KOY50693.1| Glutamate synthase (NADPH) large chain [Polaribacter dokdonensis DSW-5] >gi|1088785628|emb|SEE62819.1| glutamate synthase (NADH) large subunit [Polaribacter dokdonensis DSW-5])

HSP 1 Score: 1203.35 bits (3112), Expect = 0.000e+0
Identity = 576/610 (94.43%), Postives = 600/610 (98.36%), Query Frame = 0

Query:  124 DRQCRKLRIQNPVISNADLEKIRNIDIESFKAETIEILYPKAQGLNGLEDALDAIVVQVEKAIEKKNNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAVNPYMVNEIIRTQVKEGFITGIDEQGAVDNFNKAIGKGILKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNVGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQASYDVYAKAINEQAENLMTIRGLFQFDNLDPIPLDEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDINGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIYDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNNLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHILTTW 733
            +RQCRKLRIQNPVISNADLEKIR+I+IESFKAETI+ILYPK++GLNGLE+AL+AIV+QVEKA+E K NIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASA+NPYMVNEIIR QVKEGFIT +DEQ AVDNFN AIGKG+LKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLN+GGDYRWRRNGERHLFNPTTV+KLQQAVRLSDQ SYDVYAKA+NEQAENLMTIRGLF+FDNLDPIPL+EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKD NGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLI+DLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLI+GYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMN+LRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEH++  +
Sbjct:  549 ERQCRKLRIQNPVISNADLEKIRSINIESFKAETIQILYPKSEGLNGLENALEAIVIQVEKALEAKKNIIILSDRGVNQELAPIPALLACSFVNHQLNRLRKRSYFDIIIESAEPREPHHFATLFGYGASAINPYMVNEIIRMQVKEGFITDMDEQKAVDNFNTAIGKGLLKVMNKIGISTLHSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEKEIDQRYKQAYPDNQIDKNLGLNIGGDYRWRRNGERHLFNPTTVSKLQQAVRLSDQKSYDVYAKAVNEQAENLMTIRGLFEFDNLDPIPLEEVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRKRFQKDSNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMAQGAKPGEGGQLPGYKVLPWIANARNSTPFVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAKAKADVVLISGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVMNSLRSRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFF 1158          

HSP 2 Score: 252.677 bits (644), Expect = 8.846e-67
Identity = 120/154 (77.92%), Postives = 121/154 (78.57%), Query Frame = 0

Query:    1 MPEDIGPYYKDLQEKDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR-------------------------------GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMTKERKAFYEYNGCIMEPWDGPASVPFTD 123
            MPEDIGPYYKDLQE DLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLR                               GKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATM+ ERKAFYEYNGCIMEPWDGPASVPFTD
Sbjct:  199 MPEDIGPYYKDLQEIDLVTRLALVHQRFSTNTMPTWELAQPFRHMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIDKLFPIILPGKSDSASMDMVVELLTHTGRSLPEIMMMMIPEAWEKHATMSPERKAFYEYNGCIMEPWDGPASVPFTD 352