acyl- synthetase, maker-scaffold2333_size17043-snap-gene-0.0 (gene) Tigriopus kingsejongensis

Overview
Nameacyl- synthetase
Unique Namemaker-scaffold2333_size17043-snap-gene-0.0
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 489.574 bits (1259), Expect = 2.803e-162
Identity = 252/550 (45.82%), Postives = 329/550 (59.82%), Query Frame = 0
Query:  539 VYRKSVRNPEAFWEEIAEEHFAWRKKWDNVLSWDFKKPEGKWFEGAKLNITENCIDRHLRT-----------------------------------REITMKEGRDEWL----QPL-----------------------LDEAY----HDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIP--FSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEII 1020
            +Y  S+  P+ FW  +A +   W K +   +  + K    KWF    LN + NC+DRHL+                                    R I +KE  DE +    QP+                       L++A      +C  E M +EDPLF LYTSGSTG PKG++H+TAGY++YT++T K +F + E D++ C ADIGWITGHSYIVYGPL+NG T+V+FE  P+YPD  R+W +V++  ITQFYT+PTAIR L K     V   D SS++ LGSVGEPIN EAW+WYN+ +G+ +  IVDTWWQTETGGI ITP P   +    P     PF+G+ P L+D+ G EIK N   G LC+K PWP MAR+I+ +H+RY++TYFSA+   YFTGDGA R + GY  I GR+DDVI VSGH LGTA +ED + +H  + E+A+VG PHDIKG A+Y ++  K+  E        K I +L+ D I   A  +K QF   LPKTRSGKIMRRILRKIA  + D+ GD STL NP +V +I 
Sbjct:   43 LYNYSIERPDIFWGNLARDSITWMKNFQTTMDCNMKTASFKWFSDGLLNASVNCVDRHLKNANKTALIWEKDELGEREFNCKANVIITVDEGYRGGRVIPLKEVVDEAIKKSSQPIKHVLVAKRSGKGHVKMKSGRDVYLEDAMLQVESECHPESMSSEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPTYPDPSRYWKLVEELGITQFYTSPTAIRQLMKYEDSYVLNSDKSSLRTLGSVGEPINIEAWNWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNEIKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGV--KKFEKGIQKLVKDNISGFAVPEKFQFCEDLPKTRSGKIMRRILRKIAIGD-DSFGDXSTLANPSIVDKIF 589          

HSP 2 Score: 253.832 bits (647), Expect = 1.600e-73
Identity = 139/399 (34.84%), Postives = 224/399 (56.14%), Query Frame = 0
Query:  146 NELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYT--ALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLA---NMVGVELKPVKAGSASLPVCGYDIKILDENGEE-TQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQ 535
            +EL  R  +CK   +IT   G    R+IP K  VD+AI  +    KHV+V  R     +  K     Y   A++   +   P +++S  PL+ LYTSG+TG PKG++  + GY     F+ K I+   E D+F   +D+GW+ GHSYI YGPL+N  T++LFE  P   PD S +W+++ E  +   +T+PTAIR + K + +  ++   D SSL+     GE  +    NW    +   +  ++D WWQTE+G   +    + +  E+ P        P  G +  +LD+ G E  + + +G + +K P  PG   +++ + +R+++ Y + FPG+Y +GDG Y+ ++GY+ I GR+DD+INV+GHRL TA++E+++  H  + E AV G   +IKG+
Sbjct:  114 DELGEREFNCKANVIITVDEGYRGGRVIPLKEVVDEAIKKSSQPIKHVLVAKRSGKGHVKMKSGRDVYLEDAMLQVESECHPESMSSEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPT-YPDPSRYWKLVEELGITQFYTSPTAIRQLMKYEDS--YVLNSDKSSLRTLGSVGEPINIEAWNWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVP---ALGMRPFLGINPVLLDDKGNEIKENNKQGSLCLKTPW-PGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGE 505          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000006946 (supercontig:LSalAtl2s:LSalAtl2s393:601207:603901:-1 gene:EMLSAG00000006946 transcript:EMLSAT00000006946 description:"maker-LSalAtl2s393-augustus-gene-5.43")

HSP 1 Score: 473.011 bits (1216), Expect = 4.561e-155
Identity = 245/543 (45.12%), Postives = 338/543 (62.25%), Query Frame = 0
Query:    2 TYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDL-GLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTA--VSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV--ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
             Y+  +Q SIK+PE +W   +QN  W+ P   +L      + +WF D +L++ Y  +D+H+++GFG Q A+++DSP+T++KR +TY+ L  EV++ A  L+D   + KGD VIIYMPMI + + AMLA ARLG IHSVVFGGF+  EL++RI   + K VITAS GIE  R+I YKP VD+A+I A+     VIV+ R+       KP  D ++  L+  ++   +  V V SN PLY+LYTSGTTG+                           GD ++  SD+GW VGH Y+ Y PLL+RNT+I+FEGKP  TPDA  F RVI+E++VK  F APT +R +K+EDPN +   KY  +SLK  F+ GERCD +T  W     KVP++DHWWQTE+  PM  + +G+   L P K  S   PV G+DI+++DENG+       G +  KLP+ PG L  L+GN E F   Y + FPGFY + D G  + +GYV    R DD+INVAGHRLS + +EE++ SH  V +CAV G+  E+KGQ P+
Sbjct:   31 VYEKEFQKSIKDPEEYWGNIAQNTIWHKPWTRVLDNSNPPFSKWFVDAELSLVYNAVDRHVDEGFGSQTALVWDSPITNSKRSLTYSDLKKEVSQFAKVLRDTYDVKKGDRVIIYMPMILETIIAMLAVARLGAIHSVVFGGFSAKELSLRIKHIEAKLVITASCGIEPNRLIKYKPIVDEALIRAEVPELPVIVYQRENICQAELKPGRDSEWQDLIEGASVGDLDCVPVLSNDPLYVLYTSGTTGR-------------------------HPGDAWFTYSDMGWAVGHGYVCYSPLLHRNTSIVFEGKPAGTPDAGQFSRVIAENQVKGFFLAPTGLRFLKREDPNFEQGSKYPSNSLKAIFVGGERCDNSTKEWASHHFKVPILDHWWQTETAHPMTGHCIGLGNTLNPPK-DSTGRPVPGFDIRVVDENGKNVANGEMGRIVCKLPIAPGCLSTLYGNDEGFVGTYFSRFPGFYDTMDAGXIESNGYVKXLARDDDVINVAGHRLSISRLEEVLISHPDVVDCAVVGVRDELKGQIPI 547          

HSP 2 Score: 198.749 bits (504), Expect = 5.791e-54
Identity = 127/404 (31.44%), Postives = 202/404 (50.00%), Query Frame = 0
Query:  604 MKEGRDEWLQPLLDEA-YHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPS-YPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPI-----PFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFP-WPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIA 997
            +K GRD   Q L++ A   D D   + + DPL++LYTSG+TG   G                         D ++  +D+GW  GH Y+ Y PL++  T+++FEG P+  PD G+F  V+ ++++  F+ APT +R L ++    E   KY  +S+K +   GE  +     W + +    K PI+D WWQTET   M           + P   T   +P  G    ++DENG+ +   ++ GR+  K P  P    T++GN + +  TYFS +   Y T D    +  GY +   R DDVI V+GH L  + +E+ +  H  V + A+VG   ++KG    G I LK      D  ++++E+ Q +   +G +A L  +   + LP+TRSGKI R+ +  +A
Sbjct:  236 LKPGRDSEWQDLIEGASVGDLDCVPVLSNDPLYVLYTSGTTGRHPG-------------------------DAWFTYSDMGWAVGHGYVCYSPLLHRNTSIVFEGKPAGTPDAGQFSRVIAENQVKGFFLAPTGLRFLKREDPNFEQGSKYPSNSLKAIFVGGERCDNSTKEWASHHF---KVPILDHWWQTETAHPMTGHCIGLGNTLNPPKDSTGRPVP--GFDIRVVDENGKNVANGEM-GRIVCKLPIAPGCLSTLYGNDEGFVGTYFSRFPGFYDTMDAGXIESNGYVKXLARDDDVINVAGHRLSISRLEEVLISHPDVVDCAVVGVRDELKGQIPIGLIVLKKHFHQEDLSSLKEELVQQVRSNVGAVAXLKILLSVNNLPRTRSGKITRKCIADMA 608          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000006577 (supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-1 gene:EMLSAG00000006577 transcript:EMLSAT00000006577 description:"maker-LSalAtl2s360-snap-gene-1.18")

HSP 1 Score: 421.009 bits (1081), Expect = 1.689e-136
Identity = 198/357 (55.46%), Postives = 262/357 (73.39%), Query Frame = 0
Query:  605 KEGRDEWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQ-EDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQL 960
            K  RD W   +++ A    D+E M++EDPLF+LYTSGSTG PKG+LHTTAGYM+Y A TFK VF YQ E DVYWCTADIGWITGH+Y+ YGPL+NG+ +V+FEG P YP   RFW+++QK+ +++FYTAPTAIRAL K G + V KYDLSS+KVLG+VGEPIN EAW WY++N+G  +  IVDT+WQTETGG ++TP+P  TPTKP  A  PF GV P ++DE+G+ + G + +G +    PWP M R+++GNH+R++ TYFS +   Y +GDGA RD+ GY  ITGR+DD++  SGH + TA +E A+ EH+ V+E+A+V  PH IKG  LY +IT     +   +D M KE+  L
Sbjct:  192 KTERDHWWHEVMEPA---SDVEWMNSEDPLFLLYTSGSTGKPKGVLHTTAGYMIYAATTFKYVFDYQDESDVYWCTADIGWITGHTYVTYGPLLNGSKSVLFEGTPFYPTNDRFWEIIQKYSVSKFYTAPTAIRALMKFGEDFVSKYDLSSLKVLGTVGEPINPEAWFWYHKNVGGSQCSIVDTYWQTETGGHIMTPLPGCTPTKPGSACFPFFGVSPVILDESGKVLSG-EAEGYIVFNKPWPGMMRSVYGNHERFEKTYFSRFPGYYMSGDGAKRDKDGYIWITGRIDDMLNCSGHLMSTAQVESALVEHRHVAEAAVVSTPHPIKGECLYCFIT--PMADISLNDKMVKELKNL 542          

HSP 2 Score: 325.865 bits (834), Expect = 2.045e-100
Identity = 198/556 (35.61%), Postives = 291/556 (52.34%), Query Frame = 0
Query:    2 TYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYN-QLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERII-----PYKPFVD---------DAIILAQHKPKHVIVFDRKKGVDIPHK---PYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGV-DEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQ 535
            TYQ +Y +SI NP SFW K +    W   P      DE+                                            + YN  L+ EV + +  L+  G+ KGD V IYMPMI + + AMLACAR+G +HS+VFGG++ + LA RI D   K +I+A       ++I      YK   +         D  IL  H P+     +    ++ P +     D  +  ++  ++ V    + S  PL++LYTSG+TGKPKG++  + GY      + KY++   DE DV+W  +D+GW+ GH+Y+ YGPLLN + ++LFEG P   P    FW +I ++ V   +TAPTAIRA+ K     DF+ KYDLSSLK     GE  +     W  + +   +  ++D +WQTE+G  ++  + G    P K GSA  P  G    ILDE+G+    + EGY+    P P G + +++GN ERF+  Y + FPG+Y SGDG  +D+DGY++ITGR+DD++N +GH +STA +E  +  H+ VAE AV      IKG+
Sbjct:   22 TYQLMYDESINNPISFWGKIASQYHWKKSPS----SDEF--------------------------------------------LKYNFDLHMEVCKFSNVLKSKGVQKGDRVAIYMPMIIELVVAMLACARIGAVHSIVFGGYSSDSLASRIVDGGAKVLISADGVYRGNKLINLLDVAYKAMEETSSNYNHDIDIHILVSHIPR----LNSGSPLETPSRWKTERDHWWHEVMEPASDVE--WMNSEDPLFLLYTSGSTGKPKGVLHTTAGYMIYAATTFKYVFDYQDESDVYWCTADIGWITGHTYVTYGPLLNGSKSVLFEGTPFY-PTNDRFWEIIQKYSVSKFYTAPTAIRALMKF--GEDFVSKYDLSSLKVLGTVGEPINPEAWFWYHKNVGGSQCSIVDTYWQTETGGHIMTPLPGC--TPTKPGSACFPFFGVSPVILDESGKVLSGEAEGYIVFNKPWP-GMMRSVYGNHERFEKTYFSRFPGYYMSGDGAKRDKDGYIWITGRIDDMLNCSGHLMSTAQVESALVEHRHVAEAAVVSTPHPIKGE 517          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000006845 (supercontig:LSalAtl2s:LSalAtl2s387:339966:342548:1 gene:EMLSAG00000006845 transcript:EMLSAT00000006845 description:"snap_masked-LSalAtl2s387-processed-gene-3.14")

HSP 1 Score: 104.76 bits (260), Expect = 3.653e-23
Identity = 128/550 (23.27%), Postives = 233/550 (42.36%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWE---KQSQNLAWYTPPKHILGKDEYN--YPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQ-DLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVF----DRKKGVDIPHKPYDI----DYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKY-IYGVDEG-DVFWAASDVGWVVGH---SYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPER---FKDGYLNTFPGFYSSGDGGYKD--EDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFG 527
            Y  ++  S+K+ + FW        NL +   P+      + +   P WF D ++N +   +    E                H     T+ +L + VAR+A  L+    +  GD V+ Y+P  P+AL  MLA A LG I S     F    +  R     PK + +        +   +   + D  ++    P  V+V       KK VD+     ++    D+ + V ++  +    V  +HPL+I+++SGTTG PK II  +GG  T LK   ++ I G  +  DV    +  GW++ +   S +A G     +T ++++G P+  P  S  W ++ E  + V  T+   I   +++        K  L +LK     G      + ++  +++K  ++        G  ++A  +G   + PV  G       G DI+  +  G++ +    G +    P  P   +  W + E+   ++  Y + +P  ++ GD    +   +G+V + GR D  +N  G R  +A++  IV +   +A+    G
Sbjct:   53 YFELHSWSVKHYKDFWSFLVGSHLNLLYSGSPEVAYSNKKMDDLPPHWFPDLKINYAENLLKYENEKKIAYYYTTERMLKEGHQINKATFGELKNRVARIAYALKHKFDVKSGDRVVGYIPNCPEALEIMLASASLGAIWSSTSPDFGTVGVLDRFKQINPKVIFSIEAVSYNLKTHNHLGKLKD--VVKGLSPAAVVVIPFLAKDKKEVDLSSIHGNVMFMEDFLSSVPENPDLKFERVPFSHPLFIMFSSGTTGVPKCIIHSTGG--TLLKHVEEHQIQGNRKAQDVVLYYTTTGWMMWNWLXSALAIG-----STLVIYDGSPLH-PTHSALWDLVDECGITVFGTSAKWIAFQEEKGIKPRLTHK--LKTLKTILSTGSPLKPHSYDYVYREIKTDLL--LASISGGTDIIACFMGENSVLPVHKGEIQHSHLGCDIQCFNYGGKKEEKGTAGDLVCASPF-PSMPVGFWNDDEKHSAYRSAYFDKYPHVWAHGDYLVINPKTNGFVML-GRSDGTLNPNGVRFGSAEIYNIVETFSEIADSLCVG 586          

HSP 2 Score: 93.9745 bits (232), Expect = 8.517e-20
Identity = 95/405 (23.46%), Postives = 169/405 (41.73%), Query Frame = 0
Query:  622 DCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS----TPTKPTYATLPFIGVQPTLMDENG-QEIKGNQVDGRLCIKFPWPSMARTIWGN---HQRYKDTYFSAYENMYFTGD-GALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVG--FPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFT-SGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPE 1014
            D   E +    PLFI+++SG+TG PK ++H+T G ++      +     +  DV       GW+  +   +   L  G+T V+++G P +P     WD+V +  IT F T+   I    ++G +    + L ++K + S G P+   ++ +    I        D    + +GG  I           P          +G      +  G +E KG    G L    P+PSM    W +   H  Y+  YF  Y +++  GD   +  +   + + GR D  +  +G   G+A I + +     +++S  VG   P D +G      + LK   +    D +   +   I   +  +  L  + F    +P T +GK +   ++KI   +  ++ + S L NPE
Sbjct:  284 DLKFERVPFSHPLFIMFSSGTTGVPKCIIHSTGGTLLKHVEEHQIQGNRKAQDVVLYYTTTGWMMWN--WLXSALAIGSTLVIYDGSPLHPTHSALWDLVDECGITVFGTSAKWIAFQEEKGIKPRLTHKLKTLKTILSTGSPLKPHSYDYVYREIK------TDLLLASISGGTDIIACFMGENSVLPVHKGEIQHSHLGCDIQCFNYGGKKEEKGTA--GDLVCASPFPSMPVGFWNDDEKHSAYRSAYFDKYPHVWAHGDYLVINPKTNGFVMLGRSDGTLNPNGVRFGSAEIYNIVETFSEIADSLCVGQRSPMD-RGGEERVLLFLKMKPQHSMSDELISRLKSSIRSGLS-VRHLPSLFFQIDDIPYTINGKKVEVAVKKILAGQ--SVKNQSALANPE 674          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 86.6557 bits (213), Expect = 2.346e-17
Identity = 79/296 (26.69%), Postives = 132/296 (44.59%), Query Frame = 0
Query:  722 QKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIG-VQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAY----ENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLN 1012
            Q+ K T  +  PT   A+ +   +L  K D+SS+++  + G     E    + +++  K   +++ +  TETG     P P  +         P +G V+  ++D+NG+ +  N          P     RT    H  YKD   S      +  Y +GD A+  E GY +I GR+DD++   G N+    IED + +H  + E  I+G P D  G  L  +I L++  +  D       + +    +I          F    PKT SGK+ +  +R I+   I+ LGD ST+++
Sbjct:  652 QEFKCTYLFGTPTMYVAVLEAQKKL--KVDVSSVELGITGGSNCLVEVCEDFEKHLNMKS--LMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGKILPVNT---------PGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGAKEFD-------LKEHCKGKIAHFKIPKYSMFVKDFPKTLSGKVKKLEMRGIS---IEKLGDASTIIS 924          

HSP 2 Score: 78.5666 bits (192), Expect = 6.062e-15
Identity = 76/297 (25.59%), Postives = 131/297 (44.11%), Query Frame = 0
Query:  723 KHKITQFYTA--PTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIG-VQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAY----ENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLN 1012
            K KI  F     P  ++   K  +  V K+++ +IK+  + G     EA   + +++  K   +++ +  TETG +     P  +         P +G ++  ++D+NG+ +  N          P     RT    H  YKD   S      +  Y +GD  +  E GY +I GR+DD++I  G N+    IED + +H ++ E  IVG P +  G  L  +I L++  +  D   +++     IS    P   +    F    PKT SGK+ +  ++ I+   I+ LG     +N
Sbjct:  278 KGKIAHFKIPKYPMFVKDFPKTLSGKVKKFEMKAIKLGITGGSNCLVEACKDFEKHLNMKS--LMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKILPVNT---------PGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGAKEFD---LKEHCKGKISHFKIPKYPM----FVKDFPKTLSGKVKKFEMKAIS---IEKLGLKKATVN 553          

HSP 3 Score: 68.9366 bits (167), Expect = 5.571e-12
Identity = 61/245 (24.90%), Postives = 101/245 (41.22%), Query Frame = 0
Query:  786 DTWWQTETGGIMITPIPFSTPTKPTYATLPFIG-VQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAY----ENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKI-------------ACNEIDNLGDTSTLLN 1012
            + +  TETG +     P  +         P +G ++  ++D+NG+ +  N          P     RT    H  YKD   S      +  Y +GD  +  E GY +I GR+DD++I  G N+    IED + +H ++ E  IVG P D  G  L  +I L++  +  D       + +    +I          F    PKT SGK+ +  ++ I             AC + +   +  +L+N
Sbjct:  113 NIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKILPVNT---------PGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGAKEFD-------LKEHCKGKIAHFKIPKYPMFVKDFPKTLSGKVKKFEMKAIKLGITGGSNCLVEACKDFEKHLNMKSLMN 341          

HSP 4 Score: 54.6842 bits (130), Expect = 1.390e-7
Identity = 41/180 (22.78%), Postives = 85/180 (47.22%), Query Frame = 0
Query:  350 IKKYDLSSLKYQFLAGERCDAATLNWTEQQLKV-PVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGI 528
            +KK+++ ++K     G  C        E+ L +  +++ +  TE+G P+       +   V+  +   P+   + KI+D+NG+    +  G + V+ P   G +   + + +  K   +     +Y SGD G   E GY  I GR+DD++   G  +   ++E+++  H ++ E  + G+
Sbjct:  304 VKKFEMKAIKLGITGGSNCLVEACKDFEKHLNMKSLMNIYGATETG-PVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKILPVNTPGELWVRTP---GLMHGYYKDQKSSKK--VIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGV 477          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000012891 (supercontig:LSalAtl2s:LSalAtl2s981:104426:107346:-1 gene:EMLSAG00000012891 transcript:EMLSAT00000012891 description:"maker-LSalAtl2s981-augustus-gene-1.58")

HSP 1 Score: 67.3958 bits (163), Expect = 1.445e-11
Identity = 111/484 (22.93%), Postives = 197/484 (40.70%), Query Frame = 0
Query:   86 TYNQLYDEVARLAGGL-QDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGV---DIPHK--PYDIDYTALVNKS----TAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDV------FWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTE--QQLKVPVIDHWWQTESGWPMLANM------VGVELKPVKAGSASLPVCGYDIKI--------LDENGEETQTDV----EGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPG----FYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVAS-HKAVAECAVFGI 528
            T+  +  E +R+A     ++GL KGD V +YM   P+ +Y  L  A++G+I ++V      + +   +     KA+I    GIE++        VD+          +  ++  K GV   DI  K  P   D+  +V+KS     A   ++++ +  ++ +YTSGTTG PK  ++ +      +  ++K +  ++  D        + AS +   +G+++   G ++ +           K   A  FW+   ++KV          R +    P    I +    S++  +  G R +     W    ++ K+P++  ++ +  G   L N       VG     +      L    + IK+         DENG   Q DV    EG   + +   P    N + N E   +  L         F+ SGD   +DE GY++   RV D     G  +ST ++E    S      E AV+G+
Sbjct:  147 TFEMIDQESSRVANYFSNNVGLKKGDCVALYMTNRPEFIYITLGLAKIGVIAALVNSHLRQDSMVYALKAGNAKALIY---GIELQDA------VDEMEKSLLDLSPNFTLYCSKSGVSPEDITRKRSPVSKDFDEIVSKSPANRGASQNISLSPSSVIFYIYTSGTTGLPKP-VKITHMRQMVILMAIKNLLDLNSEDTSYCYLPLYHASGIQLGIGNAFANGGKVVIK-----------KKFSARNFWKDCVKYKVTATQYIGEICRFLLATKP----IPEETQHSIRIIYGNGLRPEI----WRSFVERFKIPLVGEFYGSTEGNMNLINFDNTVGAVGCIPNIIPKFLIELITPIHLIKVNPQTLEVYRDENGLCVQCDVGEIGEGIGRI-VKGHPVKEFNGYANEEENGNKILRDVLKKGDIFFRSGDLLVQDELGYLYFKDRVGDTFRWKGENVSTYEVEMTANSLLDNQTEVAVYGV 600          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 66.2402 bits (160), Expect = 2.904e-11
Identity = 87/392 (22.19%), Postives = 155/392 (39.54%), Query Frame = 0
Query:  617 DEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPL--INGATTVMFEGVP------SYPDFG--RFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPT-YATLPFI--GVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDK-IQFTSGLPKTRSGKIMRRILR 994
            D ++ D    I   +D L + Y+SG+TG PKG+  T +  +        N+ Q     ++    D   +  H  I   P   I   T  +  G+       + P F    F + + ++K T     P  +  +    + L+    LSSIK+L              +            D  +Q   G    +P+    P     Y +   +       ++D +  ++ G   +G LC+  P     + + G ++  K T          TGD A  ++ G + I  R+ ++I V G  +  + +ED I ++  V + A VG P +  G     Y+  K      +   M + I   +++++ P  KL   + F   LPK ++GKI+RR L+
Sbjct:  209 DGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLV-------NNITQLSHPSIHNFIGD--EVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTS--SPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDS------DLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGP-----QIMQGYYKNKKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRK------NRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 60.8474 bits (146), Expect = 1.852e-9
Identity = 94/390 (24.10%), Postives = 157/390 (40.26%), Query Frame = 0
Query:  629 DAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVV--QKHKITQFYTAPTAIRALAKQ-------GTELVDKYDLS-SIKVLGSVG--EPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIK-GNQVD--------GRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVD-DVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKI 996
            D  D   ILYTSG++G+PKG+  T        +   K  + +  DD       +  + G    +  PL  GA   M  G     D  + W  +      I+ F   PT    L K+        TE+ D    +  + V GS    EP+  + W   + +I      +++ +  TE G  +   +       P +   PF  V+  +M E G  +   N V+        G L I+   PS+    +GN +  K++ F    + + TGD A  +  G ++I GR   D+I   G  L    IE     +  +SE A+VG   +I G  +   I + + G   + D +R         ++ P +   +    + + K   GKI ++ L+K+
Sbjct:  145 DPNDGALILYTSGTSGSPKGVWITHRNIEAQVSGMIK-FWAWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGF----DAKKVWSYLTNSSMNISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRK-WQTISGHI------LLERYGMTEIGMGLGNSLE-EDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIR--GPSVFSFYYGNEKATKES-FDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGE-IVAAIIVPNKGYYLNSDILRG----FGYKKLPPYSVPRQFMLVNSIDKNHMGKINKKELKKM 513          
BLAST of acyl- synthetase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 52.7582 bits (125), Expect = 4.471e-7
Identity = 79/388 (20.36%), Postives = 148/388 (38.14%), Query Frame = 0
Query:  630 AEDPLFILYTSGSTGTPKGMLHTTAGYM--VYTAYTFKNV-FQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVL----GSVGEPINEEAW------HWYNENIGKKKSPIVDTWWQTETGGIMITP----------IPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLD-KIQFTSGLPKTRSGKIMRRIL 993
            A+D +++ +TS S+G  KG+LHT    M   Y+     N  F     D   C      +TG      G +   +   + E          F + +   K          IR L++  + LV  YDLSS+KVL    G +   I  E         +  E  G  +  +V   +  E    + +           IP   P    Y+ +  I  Q      +G  + G    G +C+K P     + + G   + + + F   +  + TGD    D  G      +++++I    + +  + +E  +     + ++A+VG P+   G     ++ LK   E  + +     +  L+  R+    ++   + F   +P+  + K++R +L
Sbjct:  222 AKDVIYMPFTSTSSGA-KGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQ-----NFLEALVDXKTASISCYAWQIRILSQ--SPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPN--MYSMIKIISRQ------DGNPVDGPDQQGEICVKSP-----QCMLGYLNKEESSAFDN-DQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEEN-----LTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVL 582          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|12231045|sp|P28812.2|Y3568_PSEAE (RecName: Full=Uncharacterized protein PA3568)

HSP 1 Score: 638.647 bits (1646), Expect = 0.000e+0
Identity = 295/538 (54.83%), Postives = 392/538 (72.86%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            MTY   +  SI  P +FW +Q+++LAWY  P +IL        +WFADG+LN +YL +D+ IE+G G+Q A+IYDSPVT T+   +Y +L DEVARLAG L+ LG+ KGD VIIYMPM+PQA  AMLACARLG +HSVVFGGFAP ELA+RIDD  PK V+TAS G+E +R+I YKP VD A+ LA H+P HV+V+ R +     H   D+D+   +  +    P+ VAS  PLYI+YTSGTTGKPKGI+RD+GG+A A++++++ IYG+  GDV+W  SDVGWVVGHS I YGPL+   TT+ +EGKP++TPDA  +WRVI EH+V  +F APTAIRAI+KEDP+G+ +K+YDL SL++ FLAGE+ D++T +W E+    PV DHWWQTE+GWP+ A  +G+    ++AGS +  V GY +++LD+ G     + +G + + LPLPPG    LWG+  R+   YL  +PG+Y +GDGGY DEDG+V+I GR DD+INV+GHRLST +MEE VA H+AVAECAV G+  E+KG  PL
Sbjct:    1 MTYLQQHARSIAEPAAFWAEQARSLAWYQAPANILESLPDGTHRWFADGRLNSAYLALDRQIEEGRGEQTALIYDSPVTGTQDRYSYLRLRDEVARLAGALRALGVGKGDRVIIYMPMVPQAAMAMLACARLGAVHSVVFGGFAPYELALRIDDATPKLVLTASCGLEFDRVIEYKPLVDKALELAIHQPAHVMVWQRPQAPARLHPGRDLDWQDCLAAAEPADPLPVASGDPLYIMYTSGTTGKPKGIVRDNGGHAVAVRYAVRTIYGMQAGDVWWGISDVGWVVGHSLIVYGPLMCGCTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAPTAIRAIRKEDPHGERVKRYDLGSLRHLFLAGEKLDSSTQHWLEEHTGRPVHDHWWQTETGWPVTAPCIGIAGHDLRAGSTNRAVPGYHVQVLDDEGRPLGANRQGAIVIALPLPPGCAQTLWGDHPRYLQAYLGNYPGYYHTGDGGYLDEDGFVYIMGRTDDVINVSGHRLSTGEMEERVAQHQAVAECAVIGVRDELKGHVPL 538          

HSP 2 Score: 295.434 bits (755), Expect = 8.665e-87
Identity = 163/423 (38.53%), Postives = 239/423 (56.50%), Query Frame = 0
Query:  612 LQPLLDEAYHDC-------DIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGT--ELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPI----PFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWP-SMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            L P  D  + DC       D   + + DPL+I+YTSG+TG PKG++    G+ V   Y  + ++  Q  DV+W  +D+GW+ GHS IVYGPL+ G TTV +EG P   PD G +W V+++H++   + APTAIRA+ K+    E V +YDL S++ L   GE ++    HW  E+ G+   P+ D WWQTETG  +  P             T   +P   VQ  ++D+ G+ +  N+  G + I  P P   A+T+WG+H RY   Y   Y   Y TGDG   DE G+  I GR DDVI VSGH L T  +E+ + +HQAV+E A++G   ++KG    G + LKD       + +++E+  L+ ++IG +A   ++     LPKTRSGKI+R +LRKIA  E  +    ST+ +P ++ EI
Sbjct:  205 LHPGRDLDWQDCLAAAEPADPLPVASGDPLYIMYTSGTTGKPKGIVRDNGGHAVAVRYAVRTIYGMQAGDVWWGISDVGWVVGHSLIVYGPLMCGCTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAPTAIRAIRKEDPHGERVKRYDLGSLRHLFLAGEKLDSSTQHWLEEHTGR---PVHDHWWQTETGWPVTAPCIGIAGHDLRAGSTNRAVPGYHVQ--VLDDEGRPLGANR-QGAIVIALPLPPGCAQTLWGDHPRYLQAYLGNYPGYYHTGDGGYLDEDGFVYIMGRTDDVINVSGHRLSTGEMEERVAQHQAVAECAVIGVRDELKGHVPLGLVVLKDDA-GIAAEQLQRELVALVREQIGALACFQRVVVVKRLPKTRSGKILRAVLRKIADGE--DYAAPSTIDDPTILGEI 618          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|75061996|sp|Q5REB8.1|ACSS3_PONAB (RecName: Full=Acyl-CoA synthetase short-chain family member 3, mitochondrial; Flags: Precursor)

HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0
Identity = 285/560 (50.89%), Postives = 380/560 (67.86%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL---VYRKSVRNPEAFWEEIAEE 557
            Y+  +  S+ +PE FW K ++ ++WY P    L         WF +G LNV Y  +D+HIE+G GD+ AIIYDSPVT+TK   TY ++ ++V++LAG L   G+ KGDTV+IYMPMIPQA+Y MLACAR+G IHS++FGGFA  EL+ RID  KPK V+TAS GIE  R + Y P V++A+ + QHKP  +++++R     +P  P  D+D+   + K+ +   V V S HPLYILYTSGTTG PKG+IR +GGYA  L +SM  IYG+  G+V+WAASD+GWVVGHSYI YGPLL+ NTT+L+EGKP+ TPDA  ++RV++EH V  +FTAPTAIRAI+++DP     K+Y L+  K  F+AGERCD  TL W++   +VPV+DHWWQTE+G P+ A+ VG+   K    G A   V GY++ ILD+N ++ +    G + VKLPLPPG    LW N E FK  Y   FPG+Y + D GY DE+GYV++  RVDD+INVAGHR+S   +EE + SH  VA+CAV G    +KG  PL   V RK +   E   E++ EE
Sbjct:   62 YKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENKHSPSTSWFVEGMLNVCYNAVDRHIENGKGDKIAIIYDSPVTNTKATFTYKEVLEQVSKLAGVLVKHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLNWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATE---EQVLEE 618          

HSP 2 Score: 255.373 bits (651), Expect = 1.011e-71
Identity = 155/437 (35.47%), Postives = 239/437 (54.69%), Query Frame = 0
Query:  602 ITMKEGRD-EWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--------TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMART-IWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEV---VQEIIDN 1022
            + +  GRD +W + +     HDC + ++ +E PL+ILYTSG+TG PKG++  T GY V   ++  +++  Q  +V+W  +D+GW+ GHSYI YGPL++G TTV++EG P   PD G ++ V+ +H +   +TAPTAIRA+ +Q  G  L  +Y L+  K L   GE  + E   W ++N+   + P++D WWQTETG    +PI  S        TP  P  A     G    ++D+N Q++K   + G + +K P P  A + +W N + +K  YF  +   Y T D    DE GY  +  RVDDVI V+GH +    IE++I  H  V++ A+VG    +KG        L+    + + + + +EI + +   IGP+A      F   LPKTRSGKI R  L  I   +   +  TST+ +P +   V+E++  
Sbjct:  263 VPLAPGRDLDWDEEMAKAQSHDC-VPVL-SEHPLYILYTSGTTGLPKGVIRPTGGYAVMLNWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEW-SKNV--FRVPVLDHWWQTETG----SPITASCVGLGNSKTP-PPGQAGKSVPGYNVMILDDNMQKLKARCL-GNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATE-EQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKI--TSTIEDPSIFGHVEEMLKQ 685          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|74752698|sp|Q9H6R3.1|ACSS3_HUMAN (RecName: Full=Acyl-CoA synthetase short-chain family member 3, mitochondrial; AltName: Full=Acyl-CoA synthetase short-chain family member 3; Flags: Precursor)

HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0
Identity = 283/560 (50.54%), Postives = 381/560 (68.04%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL---VYRKSVRNPEAFWEEIAEE 557
            Y+  +  S+ +PE FW K ++ ++WY P    L        +WF +G LN+ Y  +D+HIE+G GD+ AIIYDSPVT+TK   TY ++ ++V++LAG L   G+ KGDTV+IYMPMIPQA+Y MLACAR+G IHS++FGGFA  EL+ RID  KPK V+TAS GIE  R + Y P V++A+ + QHKP  +++++R     +P  P  D+D+   + K+ +   V V S HPLYILYTSGTTG PKG+IR +GGYA  L +SM  IYG+  G+V+WAASD+GWVVGHSYI YGPLL+ NTT+L+EGKP+ TPDA  ++RV++EH V  +FTAPTAIRAI+++DP     K+Y L+  K  F+AGERCD  TL W++   +VPV+DHWWQTE+G P+ A+ VG+   K    G A   V GY++ ILD+N ++ +    G + VKLPLPPG    LW N E FK  Y   FPG+Y + D GY DE+GY+++  RVDD+INVAGHR+S   +EE + SH  VA+CAV G    +KG  PL   V RK +   E   E++ EE
Sbjct:   62 YKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENKHSPSTRWFVEGMLNICYNAVDRHIENGKGDKIAIIYDSPVTNTKATFTYKEVLEQVSKLAGVLVKHGIKKGDTVVIYMPMIPQAMYTMLACARIGAIHSLIFGGFASKELSSRIDHVKPKVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATE---EQVLEE 618          

HSP 2 Score: 254.988 bits (650), Expect = 1.195e-71
Identity = 155/437 (35.47%), Postives = 239/437 (54.69%), Query Frame = 0
Query:  602 ITMKEGRD-EWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--------TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMART-IWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEV---VQEIIDN 1022
            + +  GRD +W + +     HDC + ++ +E PL+ILYTSG+TG PKG++  T GY V   ++  +++  Q  +V+W  +D+GW+ GHSYI YGPL++G TTV++EG P   PD G ++ V+ +H +   +TAPTAIRA+ +Q  G  L  +Y L+  K L   GE  + E   W ++N+   + P++D WWQTETG    +PI  S        TP  P  A     G    ++D+N Q++K   + G + +K P P  A + +W N + +K  YF  +   Y T D    DE GY  +  RVDDVI V+GH +    IE++I  H  V++ A+VG    +KG        L+    + + + + +EI + +   IGP+A      F   LPKTRSGKI R  L  I   +   +  TST+ +P +   V+E++  
Sbjct:  263 VPLAPGRDLDWDEEMAKAQSHDC-VPVL-SEHPLYILYTSGTTGLPKGVIRPTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEW-SKNV--FRVPVLDHWWQTETG----SPITASCVGLGNSKTP-PPGQAGKSVPGYNVMILDDNMQKLKARCL-GNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATE-EQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKI--TSTIEDPSIFGHVEEMLKQ 685          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|171769795|sp|A7MB45.1|ACSS3_BOVIN (RecName: Full=Acyl-CoA synthetase short-chain family member 3, mitochondrial; Flags: Precursor)

HSP 1 Score: 580.482 bits (1495), Expect = 0.000e+0
Identity = 285/560 (50.89%), Postives = 382/560 (68.21%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL---VYRKSVRNPEAFWEEIAEE 557
            Y+  +  S+ +PE FW K ++ ++WY P    L         WF +G LN+ Y  ID+HIE+G GD+ AIIYDSPVT+TK  ITY ++ ++V++LAG L   G+ KGDTV+IYMPMIPQA+YAMLACAR+G IHS++FGGFA  EL+ RID  KPK VITAS GIE  R + Y P V++A+ + QHKP  V++++R     +P  P Y +D+   ++K+ +   V V S HPLYILYTSGTTG PKG++R +GGYA  L +SM  IYG+  G+V+WAASD+GWVVGHSYI YGPLL+ NTT+L+EGKP+ TPDA  ++RV++EH V  +FTAPTAIRAI+++DP     K+Y L+  K  F+AGERCD  TL W+++  +VPV+DHWWQTE+G P+ A+ +G+   K    G A   V GY++ ILD+N ++ +    G + VKLPLPPG    LW N E FK  Y   FPG+Y + D GY DE+GYV++  RVDD+INVAGHR+S   +EE + S   VA+CAV G    +KG  PL   V +K +   E   E + EE
Sbjct:   61 YKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENRHSPSTSWFVEGMLNICYNAIDRHIENGKGDKIAIIYDSPVTNTKATITYKEVLEQVSKLAGVLVKHGVKKGDTVVIYMPMIPQAMYAMLACARIGAIHSLIFGGFASKELSTRIDHAKPKLVITASFGIEPGRKVEYVPLVEEALRIGQHKPDKVLIYNRPHTDMVPLAPGYYLDWDEELSKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWSMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGALEESILSLGIVADCAVVGKEDSLKGHIPLALCVLKKDINTTE---EHVLEE 617          

HSP 2 Score: 245.743 bits (626), Expect = 2.086e-68
Identity = 144/395 (36.46%), Postives = 216/395 (54.68%), Query Frame = 0
Query:  610 EWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGG-IMITPIPF--STPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMART-IWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITL-KDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKI 996
            +W + L     HDC + ++ +E PL+ILYTSG+TG PKG++  T GY V   ++  +++  +  +V+W  +D+GW+ GHSYI YGPL++G TTV++EG P   PD G ++ V+ +H +   +TAPTAIRA+ +Q  G  L  +Y L+  K L   GE  + E   W  +     + P++D WWQTETG  I  + I    S    P  A     G    ++D+N Q++K   + G + +K P P  A + +W N + +K  YF  +   Y T D    DE GY  +  RVDDVI V+GH +    +E++I     V++ A+VG    +KG        L KD   + +H  + +EI + +   IGP+A   K  F   LPKTRSGKI R  L  +
Sbjct:  271 DWDEELSKAQSHDC-VPVL-SEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWSMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVF---RVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCL-GNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYVYVMSRVDDVINVAGHRISAGALEESILSLGIVADCAVVGKEDSLKGHIPLALCVLKKDINTTEEH--VLEEIVKHVRQTIGPVAAFRKAVFVKQLPKTRSGKIPRSTLSAL 657          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|172046857|sp|Q14DH7.2|ACSS3_MOUSE (RecName: Full=Acyl-CoA synthetase short-chain family member 3, mitochondrial; Flags: Precursor)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 280/560 (50.00%), Postives = 380/560 (67.86%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYP---QWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIP-HKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVYRKSVRNPEAFWEEIAEE 557
            Y+  +  S+ +PE FW K ++ ++WY P    L   E  YP    WF +G LN+ Y  ID+HIE+G GD+ AIIYDSPVT TK  I+Y ++ ++V++LAG L   G+ KGDTV+IYMPMIPQA+Y MLACAR+G IHS++FGGFA  EL+ RID  KPK V+TAS GIE  R + Y P +++A+ + QH+P  V+++ R     +P     D+D+   + K+ +   V V S HPLYILYTSGTTG PKG++R +GGYA  L ++M  IYG+  G+V+WAASD+GWVVGHSYI YGPLL+ NTT+L+EGKP+ TPDA  ++RV++EH V  +FTAPTAIRAI+++DP     K+Y L+  K  F+AGERCD  TL W+++  +VPV+DHWWQTE+G P+ A+ +G+   K    G A   V GY++ ILD+N ++ +    G + VKLPLPPG    LW N E FK  Y   FPG+Y + D GY DE+GY+++  RVDD+INVAGHR+S   +EE V SH  VA+CAV G    +KG  PL      ++  A  E++ EE
Sbjct:   57 YKTHFAASVADPERFWGKAAEQISWYKPWTKTL---ESRYPPSTSWFVEGMLNICYNAIDRHIENGQGDKIAIIYDSPVTDTKATISYKEVLEQVSKLAGVLVKQGVKKGDTVVIYMPMIPQAIYTMLACARIGAIHSLIFGGFASKELSTRIDHAKPKVVVTASFGIEPGRKVEYIPLLEEALRIGQHRPDRVLIYSRPNMEKVPLMSGRDLDWEEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVFRVPVLDHWWQTETGSPITASCIGLGNSKTPPPGQAGKCVPGYNVMILDDNMQKLKARSLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESVLSHGTVADCAVVGKEDPLKGHVPLALCVLKKDVNASEEQVLEE 613          

HSP 2 Score: 254.603 bits (649), Expect = 1.806e-71
Identity = 156/452 (34.51%), Postives = 239/452 (52.88%), Query Frame = 0
Query:  586 LNITENCIDRHL-----RTREITMKEGRD-EWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--------TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMART-IWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            L I ++  DR L        ++ +  GRD +W + +     HDC + ++ +E PL+ILYTSG+TG PKG++  T GY V   +T  +++  +  +V+W  +D+GW+ GHSYI YGPL++G TTV++EG P   PD G ++ V+ +H +   +TAPTAIRA+ +Q  G  L  +Y L+  K L   GE  + E   W  +     + P++D WWQTETG    +PI  S        TP  P  A     G    ++D+N Q++K   + G + +K P P  A + +W N + +K  YF  +   Y T D    DE GY  +  RVDDVI V+GH +    IE+++  H  V++ A+VG    +KG        LK    + + + + +EI + +   IGP+A      F   LPKTRSGKI R  L  +   +   +  T T+ +P +   I
Sbjct:  237 LRIGQHRPDRVLIYSRPNMEKVPLMSGRDLDWEEEMAKAQSHDC-VPVL-SEHPLYILYTSGTTGLPKGVVRPTGGYAVMLNWTMSSIYGLKPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKKVF---RVPVLDHWWQTETG----SPITASCIGLGNSKTP-PPGQAGKCVPGYNVMILDDNMQKLKARSL-GNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESVLSHGTVADCAVVGKEDPLKGHVPLALCVLKKDVNASE-EQVLEEIVKHVRQSIGPVAAFRNAVFVKQLPKTRSGKIPRSTLSALVNGKPYKV--TPTIEDPSIFGHI 674          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|226709490|sp|B8FIN2.1|ACSA_DESAA (RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme)

HSP 1 Score: 539.265 bits (1388), Expect = 3.562e-179
Identity = 254/438 (57.99%), Postives = 324/438 (73.97%), Query Frame = 0
Query:  585 KLNITENCIDRHLRTREITMKEGRDEWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEIIDN 1022
            K  + + CI       ++ MK GRD W   L  +   +C  E MD+EDPLFILYTSGSTGTPKG+LHTT GY+VYT+ T + VF Y + D+YWCTADIGW+TGHSYI+YGPL NGA T+MFEG+P+YPDF RFW +  KH++  FYTAPT +RAL +QG E V     +S+++LG+VGEPIN EAW WY+  +G+++ PIVDTWWQTETGGIMITP+P +T  KP  AT PF G+QP L+D+ G +++G   +G L +   WP++ART++G+H+R+K TYF      YF+GDGA RDE GYY ITGR+DDVI VSGH LGTA IE ++  H AVSE+A+VGFPHDIKG A+Y Y+TL    E    + +RKE++  +   IGPIA  + +QFT  LPKTRSGKIMRRILRK+ACNE+DNLGDTSTL +P VV+ +I N
Sbjct:  206 KAGMVKKCIVVKHTGADVPMKAGRDLWWHELTAKESTECPPESMDSEDPLFILYTSGSTGTPKGVLHTTGGYIVYTSLTHQYVFDYHDGDIYWCTADIGWVTGHSYIIYGPLANGAVTIMFEGIPNYPDFSRFWQICDKHQVNIFYTAPTVVRALMQQGDEPVKATSRTSVRLLGTVGEPINPEAWLWYHRVVGEERCPIVDTWWQTETGGIMITPLPGATALKPGSATRPFFGIQPALIDKEGNQLEGPG-EGYLVMLDSWPALARTVYGDHRRFKSTYFIQCPGTYFSGDGARRDEDGYYWITGRIDDVINVSGHRLGTAEIESSLVAHPAVSEAAVVGFPHDIKGQAIYAYVTLSSGYEP--SEELRKELSLFVRKDIGPIATPEVLQFTDSLPKTRSGKIMRRILRKVACNELDNLGDTSTLADPAVVEALIKN 640          

HSP 2 Score: 412.149 bits (1058), Expect = 2.784e-130
Identity = 220/546 (40.29%), Postives = 326/546 (59.71%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYP---QWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNK-STAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQ---QLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVY 540
            YQ +Y +S+ +P++FW +++Q + W+ P   +     +N     +WF DG+LNV+Y C+D+H+E   GDQ AII++      +  ITY QLY+ V R +  L+  G+ KGD V IY+PMIPQ   AMLACAR+G IHS+VF GF+P+ LA RI D +   +ITA  G+   + I  K   D+A+  A    K ++V  +  G D+P K   D+ +  L  K ST   P ++ S  PL+ILYTSG+TG PKG++  +GGY      + +Y++   +GD++W  +D+GWV GHSYI YGPL N   TI+FEG P   PD S FW++  +H+V + +TAPT +RA+ ++    + +K    +S++     GE  +     W  +   + + P++D WWQTE+G  M+  + G     +K GSA+ P  G    ++D+ G + +   EGY+ V L   P     ++G+  RFK  Y    PG Y SGDG  +DEDGY +ITGR+DD+INV+GHRL TA++E  + +H AV+E AV G   +IKGQ    Y
Sbjct:   23 YQKMYDESVNDPDAFWSREAQRIDWFEPFSKV-KNSSFNGDVDIKWFLDGKLNVAYNCLDRHLEKR-GDQTAIIFEGNEPGVEEKITYRQLYERVCRFSNVLKSCGVKKGDRVSIYLPMIPQLAVAMLACARIGAIHSIVFAGFSPDALANRITDAECTILITADEGLRGPKAIGLKEAADEAMDKAGMVKKCIVV--KHTGADVPMKAGRDLWWHELTAKESTECPPESMDSEDPLFILYTSGSTGTPKGVLHTTGGYIVYTSLTHQYVFDYHDGDIYWCTADIGWVTGHSYIIYGPLANGAVTIMFEGIP-NYPDFSRFWQICDKHQVNIFYTAPTVVRALMQQ--GDEPVKATSRTSVRLLGTVGEPINPEAWLWYHRVVGEERCPIVDTWWQTETGGIMITPLPGA--TALKPGSATRPFFGIQPALIDKEGNQLEGPGEGYL-VMLDSWPALARTVYGDHRRFKSTYFIQCPGTYFSGDGARRDEDGYYWITGRIDDVINVSGHRLGTAEIESSLVAHPAVSEAAVVGFPHDIKGQAIYAY 558          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|226709500|sp|A0LG91.1|ACSA_SYNFM (RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme)

HSP 1 Score: 539.265 bits (1388), Expect = 3.806e-179
Identity = 263/422 (62.32%), Postives = 317/422 (75.12%), Query Frame = 0
Query:  601 EITMKEGRDEWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEIIDN 1022
            ++ +  GRD      +  A  DC  E+MDAEDPLFILYTSGSTG PKG+LHTT GY+VYTA + + VF Y + D+YWCTADIGW+TGHSYI+YGPL NGATTVMFEG+P+YPD+ RFW+VV KHKI  FYTAPTAIRAL +QG   V      S+K+LG+VGEPIN EAW WY  N+G+++ PIVDTWWQTETGGI+ITP+P +T  KP  AT PF GVQP ++D  G+ + G    G L IK  WP M RT++G+H+R+K TYFS Y  +YFTGDGA RDE GYY ITGRVDDVI VSGH LGTA +E A+  H AV+ESA+VGFPHDIKG  +Y Y+TLK   E  D   +R+E+ + +   IGPIA  D IQ+  GLPKTRSGKIMRRILRKIA NEIDNLGDT+TL  P VV+++I N
Sbjct:  222 KVPVVPGRDVDWTEAIKAASPDCPPEVMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYTALSHQYVFDYHDGDIYWCTADIGWVTGHSYIIYGPLANGATTVMFEGIPNYPDWSRFWNVVDKHKINIFYTAPTAIRALMRQGEAPVRATSRKSLKLLGTVGEPINPEAWLWYYNNVGEQRCPIVDTWWQTETGGILITPLPGATALKPGSATRPFFGVQPAIIDPEGKMLDGPG-SGYLIIKESWPGMLRTVYGDHERFKQTYFSNYPGLYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAESAVVGFPHDIKGQGIYAYVTLKANWEHSDE--LRQELVKWVRKEIGPIATPDYIQWAPGLPKTRSGKIMRRILRKIAANEIDNLGDTTTLAEPAVVEDLIKN 640          

HSP 2 Score: 418.698 bits (1075), Expect = 8.480e-133
Identity = 231/570 (40.53%), Postives = 338/570 (59.30%), Query Frame = 0
Query:    2 TYQNIYQDSIKNPESFWEKQSQNLAWYTP-PKHILGKDEYNYP-QWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTAVSPVAVA-SNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVYRKSVRNPEAFWEEIAEEHFAWRKK 564
            TY  +Y+ S+K+PE FW + ++ + W+ P  K   G  E +   +WF +G+LNV+Y C+D+++    GDQ AII++       ++ITY +L+D+V R A  L+  GL KGD   IY+PMIP+   AMLACAR+G++HS+VF GF+P  LA RI DC  K VITA  G+   + IP K   ++A+       K ++V  +  G  +P  P  D+D+T  +  ++   P  V  +  PL+ILYTSG+TGKPKG++  +GGY      S +Y++   +GD++W  +D+GWV GHSYI YGPL N  TT++FEG P   PD S FW V+ +HK+ + +TAPTAIRA+ ++      ++     SLK     GE  +     W    +   + P++D WWQTE+G  ++  + G     +K GSA+ P  G    I+D  G+       GY+ +K    PG L  ++G+ ERFK  Y + +PG Y +GDG  +DEDGY +ITGRVDD+INV+GHRL TA++E  + +H AVAE AV G   +IKGQ    Y     N E   +E+ +E   W +K
Sbjct:   22 TYLKMYEQSVKDPEGFWGEHAKRIDWFQPWTKVKSGSFEGDVRFKWFENGKLNVAYNCLDRNLAK-RGDQVAIIWEGDDPKVSKYITYRELHDQVCRFANVLKAQGLKKGDRATIYLPMIPELAVAMLACARIGVVHSIVFAGFSPESLAGRILDCGGKVVITADEGLRGGKPIPLKENTEEALKKCPDVQKVIVV--KHTGGKVPVVPGRDVDWTEAIKAASPDCPPEVMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYTALSHQYVFDYHDGDIYWCTADIGWVTGHSYIIYGPLANGATTVMFEGIP-NYPDWSRFWNVVDKHKINIFYTAPTAIRALMRQGEAP--VRATSRKSLKLLGTVGEPINPEAWLWYYNNVGEQRCPIVDTWWQTETGGILITPLPGATA--LKPGSATRPFFGVQPAIIDPEGKMLDGPGSGYLIIKESW-PGMLRTVYGDHERFKQTYFSNYPGLYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAESAVVGFPHDIKGQGIYAYVTLKANWEHS-DELRQELVKWVRK 581          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|259710029|sp|B0S8X7.1|ACSA_LEPBA (RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme >gi|259710030|sp|B0SRX5.1|ACSA_LEPBP RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme)

HSP 1 Score: 536.184 bits (1380), Expect = 6.692e-178
Identity = 255/424 (60.14%), Postives = 321/424 (75.71%), Query Frame = 0
Query:  602 ITMKEGRDEWLQPLLD--EAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGN-QVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEIIDN 1022
            +  KEGRD W   L+   E   +C    M++EDPLFILYTSGSTG PKG+LHTTAGY++    TF  +F Y++ D YWCTADIGWITGHSYI+YGPL NGAT++MFEGVPSYPD GRFWDV+ K+K+T FYTAPTAIRAL ++G E + K  L+S+++LGSVGEPIN EAW WY  NIGK K PIVDTWWQTETG IMI+ IP + P KP  A+ PF G+QP L+D  G E+KG  ++ G LCI  PWPSM R ++G+ +R+ DTYFS ++  YFTGDGA RD+ GY+RITGRVDDV+ VSGH +G+A +E A+ EH++V+E+A+VGFPHDIKG  +Y Y+T+K    +  +D ++KE+  ++   IG IA+ D I +  GLPKTRSGKIMRRILRKIA NE D LGD STL +P VVQ +ID+
Sbjct:  227 LNWKEGRDHWYHYLMKDPEVKKECPAVPMESEDPLFILYTSGSTGKPKGVLHTTAGYLLGANLTFATIFDYKDTDTYWCTADIGWITGHSYILYGPLSNGATSLMFEGVPSYPDMGRFWDVIDKYKVTVFYTAPTAIRALMREGLEHIKKRSLASLRLLGSVGEPINPEAWEWYYANIGKSKCPIVDTWWQTETGSIMISGIPGAIPQKPGSASWPFYGIQPVLVDNEGVELKGKGEISGNLCIAKPWPSMMRGVYGDPKRFFDTYFSQFKGYYFTGDGANRDKEGYFRITGRVDDVLNVSGHRIGSAEVESALVEHKSVAEAAVVGFPHDIKGQGIYAYVTVKQGVVT--NDLLKKELIAMVEKVIGKIARPDVIHWAPGLPKTRSGKIMRRILRKIANNEFDTLGDISTLADPSVVQSLIDD 648          

HSP 2 Score: 394.43 bits (1012), Expect = 1.669e-123
Identity = 218/553 (39.42%), Postives = 319/553 (57.69%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYP-QWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDR---------KKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEET--QTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVY 540
            Y++ Y++SI+ PE FW +Q++ L W+     +L  D      +WF  G+LNVSY C+D+H++    ++ A+I++       + +TY+ L+ EV   A  L+   + KGD V+IY+PM+P+   A LAC R+G +HSVVFGGF+P  L  RI+DCKP  VITA  G    + I  K  VD A+   + K   VIV  R         K+G D  H  + +     V K     P  + S  PL+ILYTSG+TGKPKG++  + GY      +   I+   + D +W  +D+GW+ GHSYI YGPL N  T+++FEG P   PD   FW VI ++KV V +TAPTAIRA+ +E    + IKK  L+SL+     GE  +     W    +   K P++D WWQTE+G  M++ + G    P K GSAS P  G    ++D  G E   + ++ G + +  P  P  +  ++G+P+RF D Y + F G+Y +GDG  +D++GY  ITGRVDD++NV+GHR+ +A++E  +  HK+VAE AV G   +IKGQ    Y
Sbjct:   24 YKSKYKESIEKPEKFWAEQAKRLTWFKKWTKVLRHDFAKAKVEWFVGGKLNVSYNCLDRHLDSPLKNKAALIWEGDNPDESKVLTYHDLHREVNHFANVLKKFKVKKGDRVLIYLPMVPELAIATLACTRIGAVHSVVFGGFSPEALLGRIEDCKPTLVITADGGYRGGKPIELKKNVDAALAETKFKVNDVIVVKRTGDEGNLNWKEGRD--HWYHYLMKDPEVKKECPAVP--MESEDPLFILYTSGSTGKPKGVLHTTAGYLLGANLTFATIFDYKDTDTYWCTADIGWITGHSYILYGPLSNGATSLMFEGVP-SYPDMGRFWDVIDKYKVTVFYTAPTAIRALMRE--GLEHIKKRSLASLRLLGSVGEPINPEAWEWYYANIGKSKCPIVDTWWQTETGSIMISGIPGA--IPQKPGSASWPFYGIQPVLVDNEGVELKGKGEISGNLCIAKPW-PSMMRGVYGDPKRFFDTYFSQFKGYYFTGDGANRDKEGYFRITGRVDDVLNVSGHRIGSAEVESALVEHKSVAEAAVVGFPHDIKGQGIYAY 566          

HSP 3 Score: 69.707 bits (169), Expect = 8.408e-11
Identity = 45/121 (37.19%), Postives = 63/121 (52.07%), Query Frame = 0
Query:  511 EEIVASHKAVAECAVFGIHCEIKGQKPLVYRKSVRNPEAFWEEIAEEHFAWRKKWDNVLSWDFKKPEGKWFEGAKLNITENCIDRH----LRTREITMKEGRDEWLQPLLDEAYHDCDIEI 627
            E IVA  K  A+ A   +  E K +    Y++S+  PE FW E A+    W KKW  VL  DF K + +WF G KLN++ NC+DRH    L+ +   + EG +     +L   YHD   E+
Sbjct:    4 ERIVAPSKEFAKLANVSLK-EYKSK----YKESIEKPEKFWAEQAK-RLTWFKKWTKVLRHDFAKAKVEWFVGGKLNVSYNCLDRHLDSPLKNKAALIWEGDNPDESKVL--TYHDLHREV 116          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|166919472|sp|A0L576.1|ACSA_MAGMM (RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme)

HSP 1 Score: 532.717 bits (1371), Expect = 9.958e-177
Identity = 252/422 (59.72%), Postives = 310/422 (73.46%), Query Frame = 0
Query:  601 EITMKEGRDEWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEIIDN 1022
            EI   EGRD W    +  A  DC  E MDAEDPLF+LYTSGSTG PKG+LHTT GY++Y + T + +F Y EDD+YWCTAD+GW+TGH+YIVYGPL NGATT+MFEGVP+YPD  RFW VV KH+++ FYTAPTAIR++  QG  LV      S+++LGSVGEPIN EAW WY   +G+ + PIVDTWWQTETGGI+ITP+P +TP KP  AT PF GV P ++   G+E++G   +G L +  PWP M RT++G+H R+ +TYF+ Y   YF GDG  RDE GYY ITGRVDDVI VSGH LGTA +E A+  H  V+E+A+VG PH+IKG  +Y Y+TL    E    D ++ E+ +L+   IGPIA LD IQF  GLPKTRSGKIMRRILRKIA +E+D LGDTSTL +P VVQ +IDN
Sbjct:  223 EIEWTEGRDVWYHEAIAAASADCPPEEMDAEDPLFVLYTSGSTGKPKGVLHTTGGYLLYASITHQYIFDYHEDDIYWCTADVGWVTGHTYIVYGPLANGATTLMFEGVPTYPDASRFWQVVDKHQVSIFYTAPTAIRSIMSQGDALVKATSRQSLRILGSVGEPINPEAWEWYYHVVGEGRCPIVDTWWQTETGGILITPLPGATPLKPGSATRPFFGVNPKVLSNEGKEMEG-PCEGILVLDHPWPGMMRTVYGDHDRFINTYFANYPGYYFCGDGCRRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVLHDQVAEAAVVGMPHEIKGQGIYAYVTLMSGVEPS--DALKTELVKLVRKEIGPIASLDVIQFAPGLPKTRSGKIMRRILRKIAAHEVDTLGDTSTLADPSVVQNLIDN 641          

HSP 2 Score: 402.905 bits (1034), Expect = 7.432e-127
Identity = 223/546 (40.84%), Postives = 325/546 (59.52%), Query Frame = 0
Query:    3 YQNIYQDSIKNPESFWEKQSQ-NLAWYTPPKHILGKD-EYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIP-HKPYDIDY-TALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGD-FIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVY 540
            YQ +Y+ SI +P +FW +Q++  + W+    ++L  D    + +WF   +LNVSY CID+H+E   GD+ AI+++       R ITY +L   V + A  L+  G+ KGD V IYMPMIP+A  AMLAC R+G +HS+VFGGF+P+ L  RI D + + VITA  G+   + IP +  V+ A  LAQ    H     +   ++I   +  D+ Y  A+   S    P  + +  PL++LYTSG+TGKPKG++  +GGY      + +YI+   E D++W  +DVGWV GH+YI YGPL N  TT++FEG P   PDAS FW+V+ +H+V + +TAPTAIR+I  +   GD  +K     SL+     GE  +     W    +   + P++D WWQTE+G  ++  + G    P+K GSA+ P  G + K+L   G+E +   EG + +  P  PG +  ++G+ +RF + Y   +PG+Y  GDG  +DEDGY +ITGRVDD+INV+GHRL TA++E  +  H  VAE AV G+  EIKGQ    Y
Sbjct:   24 YQEMYKRSIDDPAAFWAEQAEARIDWFKKWDNVLDFDLRKGHIKWFEGAKLNVSYNCIDRHLEKR-GDKVAILWEGDNPAADRKITYRELSALVNKFANALKARGVKKGDRVCIYMPMIPEAAVAMLACTRIGAVHSIVFGGFSPDALRDRIIDAECRVVITADGGMRGSKKIPLRKNVETA--LAQCPNVHTCFVVQDTQLEIEWTEGRDVWYHEAIAAASADCPPEEMDAEDPLFVLYTSGSTGKPKGVLHTTGGYLLYASITHQYIFDYHEDDIYWCTADVGWVTGHTYIVYGPLANGATTLMFEGVP-TYPDASRFWQVVDKHQVSIFYTAPTAIRSIMSQ---GDALVKATSRQSLRILGSVGEPINPEAWEWYYHVVGEGRCPIVDTWWQTETGGILITPLPGA--TPLKPGSATRPFFGVNPKVLSNEGKEMEGPCEGILVLDHPW-PGMMRTVYGDHDRFINTYFANYPGYYFCGDGCRRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVLHDQVAEAAVVGMPHEIKGQGIYAY 559          

HSP 3 Score: 85.5001 bits (210), Expect = 1.011e-15
Identity = 36/62 (58.06%), Postives = 45/62 (72.58%), Query Frame = 0
Query:  539 VYRKSVRNPEAFWEEIAEEHFAWRKKWDNVLSWDFKKPEGKWFEGAKLNITENCIDRHLRTR 600
            +Y++S+ +P AFW E AE    W KKWDNVL +D +K   KWFEGAKLN++ NCIDRHL  R
Sbjct:   27 MYKRSIDDPAAFWAEQAEARIDWFKKWDNVLDFDLRKGHIKWFEGAKLNVSYNCIDRHLEKR 88          
BLAST of acyl- synthetase vs. SwissProt
Match: gi|122312288|sp|Q0AAW0.1|ACSA_ALKEH (RecName: Full=Acetyl-coenzyme A synthetase; Short=AcCoA synthetase; Short=Acs; AltName: Full=Acetate--CoA ligase; AltName: Full=Acyl-activating enzyme)

HSP 1 Score: 531.946 bits (1369), Expect = 2.401e-176
Identity = 256/422 (60.66%), Postives = 316/422 (74.88%), Query Frame = 0
Query:  601 EITMKEGRDEWLQPLLDEAYHDCDIEIMDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVPSYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEIIDN 1022
            +I  KEGRD W    + +   DC  E +DAEDPLFILYTSGSTG PKG+ H +AGY++  A T K VF YQE +VYWCTAD+GW+TGHSYIVYGPL NGATT+MFEGVP+YP   R W+VV KH ++ FYTAPTAIRAL  QG E V K    S+++LG+VGEPIN EAW WY   +G  + PIVDTWWQTETG I+I P+P +T  KP  ATLPF GV+P L+D  G+E++G    G L IK  WPSM RT++G+H+R+ +TY +AY  MYFTGDGA RDE GYY ITGRVDDVI VSGH +GTA +E A+  H AV+E+A+VG+PHDIKG  +Y Y+TL    E  D   ++KE+ +L+S+ IGPIAK D IQ+  GLPKTRSGKIMRRILRK+A NE+D+LGDTSTL +P VV  +I++
Sbjct:  223 DIDWKEGRDIWYHEAVADVSADCPAEELDAEDPLFILYTSGSTGKPKGVQHCSAGYLLGAAMTHKYVFDYQEGEVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPTYPSAARCWEVVDKHNVSIFYTAPTAIRALMGQGNEHVTKTSRKSLRILGTVGEPINPEAWEWYYNVVGDGRCPIVDTWWQTETGSILIAPLPGATDLKPGSATLPFFGVEPALVDPEGKELEG-AASGNLVIKRAWPSMMRTVYGDHKRFMETYLAAYPGMYFTGDGARRDEDGYYWITGRVDDVINVSGHRMGTAEVESALVLHDAVAEAAVVGYPHDIKGQGIYAYVTLMAGVEPSDE--LKKELVKLVSNEIGPIAKPDVIQWAPGLPKTRSGKIMRRILRKVAANELDSLGDTSTLADPTVVDNLIED 641          

HSP 2 Score: 399.053 bits (1024), Expect = 2.342e-125
Identity = 227/549 (41.35%), Postives = 326/549 (59.38%), Query Frame = 0
Query:    2 TYQNIYQDSIKNPESFWEKQSQN-LAWYTPPKHILGKDEYNYP-QWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKP--KHVIVFDRKKGVDIPHKP-YDIDYTALVNKSTAVSPV-AVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQL---KVPVIDHWWQTESGWPMLANMVGV-ELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPLVY 540
            TY+ +Y+ S+ +PE FW +Q++  L W++    +   D  N   ++F  G+LNV++ C+D+H+E   GDQ AII++    +   HITY  L++ V RLA  ++  G+ KGD V IY+PMIP+A  AMLACAR+G IHS+VFGGF+P+ L  RI +   + VITA  G+   R +  K   D A+   +  P  K+V V  R  G DI  K   DI Y   V   +A  P   + +  PL+ILYTSG+TGKPKG+   S GY      + KY++   EG+V+W  +DVGWV GHSYI YGPL N  TT++FEG P   P A+  W V+ +H V + +TAPTAIRA+  +    + + K    SL+     GE  +     W    +   + P++D WWQTE+G  ++A + G  +LKP   GSA+LP  G +  ++D  G+E +    G + +K    P  +  ++G+ +RF + YL  +PG Y +GDG  +DEDGY +ITGRVDD+INV+GHR+ TA++E  +  H AVAE AV G   +IKGQ    Y
Sbjct:   23 TYEEMYRRSLDDPEGFWGEQAEKFLDWFSKWDKVYHSDLKNGEIRFFEGGKLNVAHNCLDRHLEQ-RGDQTAIIWEGDDPNNSEHITYKDLHERVCRLANAMKARGVKKGDRVCIYLPMIPEAAVAMLACARIGAIHSIVFGGFSPDALKDRIQNADCETVITADEGVRGGRNVALKSNADKAL---ESCPDVKNVFVVKRTGG-DIDWKEGRDIWYHEAVADVSADCPAEELDAEDPLFILYTSGSTGKPKGVQHCSAGYLLGAAMTHKYVFDYQEGEVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPT-YPSAARCWEVVDKHNVSIFYTAPTAIRALMGQ--GNEHVTKTSRKSLRILGTVGEPINPEAWEWYYNVVGDGRCPIVDTWWQTETGSILIAPLPGATDLKP---GSATLPFFGVEPALVDPEGKELEGAASGNLVIKRAW-PSMMRTVYGDHKRFMETYLAAYPGMYFTGDGARRDEDGYYWITGRVDDVINVSGHRMGTAEVESALVLHDAVAEAAVVGYPHDIKGQGIYAY 559          

HSP 3 Score: 73.1738 bits (178), Expect = 5.609e-12
Identity = 31/62 (50.00%), Postives = 40/62 (64.52%), Query Frame = 0
Query:  539 VYRKSVRNPEAFWEEIAEEHFAWRKKWDNVLSWDFKKPEGKWFEGAKLNITENCIDRHLRTR 600
            +YR+S+ +PE FW E AE+   W  KWD V   D K  E ++FEG KLN+  NC+DRHL  R
Sbjct:   27 MYRRSLDDPEGFWGEQAEKFLDWFSKWDKVYHSDLKNGEIRFFEGGKLNVAHNCLDRHLEQR 88          
BLAST of acyl- synthetase vs. nr
Match: gi|1140751131|ref|WP_076615732.1| (propionyl-CoA synthetase [Winogradskyella sp. J14-2] >gi|1134992052|gb|APY07225.1| propionyl-CoA synthetase [Winogradskyella sp. J14-2])

HSP 1 Score: 953.355 bits (2463), Expect = 0.000e+0
Identity = 451/559 (80.68%), Postives = 506/559 (90.52%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKP--LVYRKSVRNPEAFWEEIAEE 557
            M+YQ+IY +SI +PESFW+KQS+ L W+  P  ILG+D+Y+YPQWF DG+LN+SYLCIDKHI+DG+G+QNAIIYDSPVT TK+HI++N+L+ EV++LAGGLQ LGL KGDT +IYMPMIPQALYAMLACARLG+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNGIEI+RIIPYKPFVD+AI LA HKP+HVIVFDR+ GVDIP++  DIDY  LV+KS A+ PV VASNHP YILYTSGTTGKPKGIIRD+GGYATALKFSMKY+YGV+EGDV+WAASDVGWVVGHSYI Y PLLNRNTTILFEGKPIKTPDASTFWRVISEH VKVMFTAPTAIRAIKKEDPNG+FI+KYDLSSLKYQFLAGERCD ATL WTEQ LKVPVIDHWWQTESGWPMLANM GVEL  VK GSASLPVCGYDI+IL+E G+E    VEGYV+VKLPLPPGTLLNLW NP+RFK+GYLN FPG+Y SGDGGYKDEDGYVFITGRVDDIINVAGHRLSTA+MEEIVASHK+VAECAVFGIHC+IKGQKP  L+  KS ++ E    EI++E
Sbjct:    1 MSYQDIYLESINHPESFWKKQSEQLEWFKAPSKILGRDQYDYPQWFQDGELNLSYLCIDKHIKDGYGEQNAIIYDSPVTDTKQHISFNELHAEVSKLAGGLQTLGLQKGDTCVIYMPMIPQALYAMLACARLGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGIEIKRIIPYKPFVDEAITLASHKPEHVIVFDRQLGVDIPYQKRDIDYLELVSKSDAIEPVKVASNHPSYILYTSGTTGKPKGIIRDTGGYATALKFSMKYVYGVEEGDVYWAASDVGWVVGHSYIVYAPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPNGEFIEKYDLSSLKYQFLAGERCDVATLRWTEQHLKVPVIDHWWQTESGWPMLANMAGVELMEVKPGSASLPVCGYDIQILNEEGKEVAAGVEGYVAVKLPLPPGTLLNLWENPDRFKNGYLNKFPGYYFSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTAEMEEIVASHKSVAECAVFGIHCDIKGQKPLGLIVLKSGKHAEE--HEISKE 557          

HSP 2 Score: 297.745 bits (761), Expect = 4.004e-85
Identity = 154/371 (41.51%), Postives = 222/371 (59.84%), Query Frame = 0
Query:  633 PLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIA 997
            P +ILYTSG+TG PKG++  T GY     ++ K V+  +E DVYW  +D+GW+ GHSYIVY PL+N  TT++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+    E ++KYDLSS+K     GE  +     W  +++   K P++D WWQTE+G  M+  +        KP  A+LP  G    +++E G+E+    V+G + +K P  P     +W N  R+K+ Y + +   YF+GDG  +DE GY  ITGRVDD+I V+GH L TA +E+ +  H++V+E A+ G   DIKG    G I LK    + +H+ + KEI Q +   IG +A    +     LPKTRSGKI+R++LR I+
Sbjct:  233 PSYILYTSGTTGKPKGIIRDTGGYATALKFSMKYVYGVEEGDVYWAASDVGWVVGHSYIVYAPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPNGEFIEKYDLSSLKYQFLAGERCDVATLRWTEQHL---KVPVIDHWWQTESGWPMLANMAGVELMEVKPGSASLPVCGYDIQILNEEGKEVAAG-VEGYVAVKLPLPPGTLLNLWENPDRFKNGYLNKFPGYYFSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTAEMEEIVASHKSVAECAVFGIHCDIKGQKPLGLIVLKSGKHAEEHE-ISKEIIQDVRHEIGAVAAFKDVFVVKRLPKTRSGKILRKLLRHIS 598          
BLAST of acyl- synthetase vs. nr
Match: gi|1317348621|ref|WP_101449387.1| (propionyl-CoA synthetase [Aquimarina sp. MAR_2010_214] >gi|1315795371|gb|PKV51742.1| propionyl-CoA synthetase [Aquimarina sp. MAR_2010_214])

HSP 1 Score: 929.087 bits (2400), Expect = 0.000e+0
Identity = 430/538 (79.93%), Postives = 489/538 (90.89%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M+Y+  Y  SI++PE FW +Q+Q L+WY  P++IL KD   Y QWFADG+LNVSYLCIDKHIEDG+G+QNAIIYDSPVT+T++HITYNQL++EV++LAGGLQ+LGL KGDTVIIYMPMIPQA+YAM ACAR+G+IHSVVFGGFAP+ELAIRI+DC+PKA+ITAS+GIE+ER+IPYKPFVD AI  A+HKP HVIV+DRK GV IPHK YD+DY +LV K+TA  PV VAS HPLYILYTSGTTG PKGIIRDSGGYATALKFSMKY+YGVDEGDV+WAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDP+G+ IK++D+S LKYQFLAGERCD ATL WTE  LK+P+IDHWWQTESGWPMLANMVG+EL+ +K GSA LPVCGYDI+IL+ENG++T T+ EGYV++KLPLPPGTL NLW NPERFK GYL  F G+Y SGDGGYKDEDGY+FITGRVDDIINVAGHRLSTA+MEE+VASHKAVAECAVFG+HC IKGQKPL
Sbjct:    1 MSYKEFYNQSIQDPEGFWREQAQKLSWYKQPENILSKDSNEYDQWFADGELNVSYLCIDKHIEDGYGEQNAIIYDSPVTNTQKHITYNQLHEEVSKLAGGLQELGLVKGDTVIIYMPMIPQAIYAMQACARIGVIHSVVFGGFAPHELAIRINDCEPKAIITASSGIEVERVIPYKPFVDKAIEEAKHKPDHVIVYDRKLGVGIPHKSYDLDYDSLVAKATAAKPVPVASTHPLYILYTSGTTGTPKGIIRDSGGYATALKFSMKYVYGVDEGDVYWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPDGELIKQFDISCLKYQFLAGERCDIATLRWTENHLKIPIIDHWWQTESGWPMLANMVGLELQEIKPGSAGLPVCGYDIQILNENGKQTPTNTEGYVAIKLPLPPGTLSNLWKNPERFKAGYLERFEGYYFSGDGGYKDEDGYLFITGRVDDIINVAGHRLSTAEMEEVVASHKAVAECAVFGVHCSIKGQKPL 538          

HSP 2 Score: 298.516 bits (763), Expect = 2.192e-85
Identity = 157/371 (42.32%), Postives = 220/371 (59.30%), Query Frame = 0
Query:  633 PLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGT--ELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPF--STPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIA 997
            PL+ILYTSG+TGTPKG++  + GY     ++ K V+   E DVYW  +D+GW+ GHSYI YGPL+N  TT++FEG P   PD   FW V+ +HK+   +TAPTAIRA+ K+    EL+ ++D+S +K     GE  +     W   ++   K PI+D WWQTE+G  M+  +        KP  A LP  G    +++ENG++   N  +G + IK P  P     +W N +R+K  Y   +E  YF+GDG  +DE GY  ITGRVDD+I V+GH L TA +E+ +  H+AV+E A+ G    IKG    G + LK TGE+ D   ++ EI   + D IG +A    +     LPKTRSGKI+R++LR I 
Sbjct:  233 PLYILYTSGTTGTPKGIIRDSGGYATALKFSMKYVYGVDEGDVYWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPDGELIKQFDISCLKYQFLAGERCDIATLRWTENHL---KIPIIDHWWQTESGWPMLANMVGLELQEIKPGSAGLPVCGYDIQILNENGKQTPTN-TEGYVAIKLPLPPGTLSNLWKNPERFKAGYLERFEGYYFSGDGGYKDEDGYLFITGRVDDIINVAGHRLSTAEMEEVVASHKAVAECAVFGVHCSIKGQKPLGLVVLK-TGETLDDQQLQNEIVTDVRDVIGAVASFKNVLVVKRLPKTRSGKILRKLLRNIV 598          
BLAST of acyl- synthetase vs. nr
Match: gi|1236591505|ref|WP_094968451.1| (acetate--CoA ligase [Winogradskyella sp. WNB302] >gi|1235776952|gb|OZV68685.1| propionyl-CoA synthetase [Winogradskyella sp. WNB302])

HSP 1 Score: 928.317 bits (2398), Expect = 0.000e+0
Identity = 441/538 (81.97%), Postives = 491/538 (91.26%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ ++ +SI NP  FWEKQ++ L+WY  PK ILG D++NYPQWF DG+LN+SYLCIDKHIEDGFG Q+AIIYDSPVT TK HI++ +L+DEV++LAGGL  LGL+KGDT+IIYMPMIPQALY+MLACARLGIIHSVVFGGFAP+ELAIRIDDCKPKAVITASNGIEI+RIIPYKPFVDDAI LA+HKP+HVIVFDR  G+++P K YDIDY+ LV KS +V P+ V SNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMK++YGV+EGDV+WAASDVGWVVGHSYI YGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDP+GDFI KYDLSSLKYQFLAGERCD +TL WTEQ LKVPVIDHWWQTESGWPMLANMVGVE  PVK GSA LPVCGYDI++L+ENG+E  + VEG+V+VKLPLPPGTLLNLWGNPERFK GYL  FPG+Y SGDGG+KDED YVFITGRVDDIINVAGHRLSTA+MEE+VASH AVAECAVFG+HC++KGQKPL
Sbjct:    1 MNYQELHIESISNPSQFWEKQAKQLSWYKAPKKILGVDDFNYPQWFVDGKLNMSYLCIDKHIEDGFGSQDAIIYDSPVTKTKEHISFYKLHDEVSKLAGGLLALGLTKGDTIIIYMPMIPQALYSMLACARLGIIHSVVFGGFAPHELAIRIDDCKPKAVITASNGIEIDRIIPYKPFVDDAINLAEHKPRHVIVFDRNLGLEMPQKSYDIDYSDLVAKSVSVEPIPVESNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKHVYGVEEGDVYWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPDGDFITKYDLSSLKYQFLAGERCDVSTLRWTEQHLKVPVIDHWWQTESGWPMLANMVGVEKMPVKPGSAGLPVCGYDIQVLNENGKEVTSGVEGFVTVKLPLPPGTLLNLWGNPERFKAGYLQRFPGYYFSGDGGFKDEDNYVFITGRVDDIINVAGHRLSTAEMEEVVASHSAVAECAVFGVHCDLKGQKPL 538          

HSP 2 Score: 300.827 bits (769), Expect = 3.817e-86
Identity = 154/376 (40.96%), Postives = 226/376 (60.11%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGT--ELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPF--STPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIA 997
            +++  PL+ILYTSG+TG PKG++  + GY     ++ K+V+  +E DVYW  +D+GW+ GHSYIVYGPL+N  TT++FEG P   PD   FW V+ +HK+   +TAPTAIRA+ K+    + + KYDLSS+K     GE  +     W  +++   K P++D WWQTE+G  M+  +      P KP  A LP  G    +++ENG+E+    V+G + +K P  P     +WGN +R+K  Y   +   YF+GDG  +DE  Y  ITGRVDD+I V+GH L TA +E+ +  H AV+E A+ G   D+KG    G + LK +    D   + ++I + +   IG +A L  +     LPKTRSGKI+R++LR I+
Sbjct:  228 VESNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKHVYGVEEGDVYWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPDGDFITKYDLSSLKYQFLAGERCDVSTLRWTEQHL---KVPVIDHWWQTESGWPMLANMVGVEKMPVKPGSAGLPVCGYDIQVLNENGKEVTSG-VEGFVTVKLPLPPGTLLNLWGNPERFKAGYLQRFPGYYFSGDGGFKDEDNYVFITGRVDDIINVAGHRLSTAEMEEVVASHSAVAECAVFGVHCDLKGQKPLGLVVLK-SENGLDETAITRDIIKEVRHEIGAVASLKDVLVVKRLPKTRSGKILRKLLRNIS 598          
BLAST of acyl- synthetase vs. nr
Match: gi|1306818290|ref|WP_100996365.1| (propionyl-CoA synthetase [Lacinutrix sp. Bg11-31] >gi|1304125096|gb|AUC81732.1| propionyl-CoA synthetase [Lacinutrix sp. Bg11-31])

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 433/538 (80.48%), Postives = 482/538 (89.59%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  YQ SI+ PE FW +Q++N+ W+  P+ IL KD+ +Y QWF DG+LN+SYLCIDKHI+DGFG QNAII+DSPVT+TK+HIT+NQL+ EV++LAGGLQ LGL KGDT IIYMPMIPQALYAMLACAR+G+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNGIEIERIIPYKPFVD+AI  A++KP+HVIVFDR  GV+IP K YDIDYT LV KS ++  V+V S HP YILYTSGTTG PKGIIRDSGGYATALKFSMKYIYGVDEGD FWAASDVGWVVGHSYI YGPLLNRNTTI+FEGKPI+TPDASTFWRVISEH+VKVMFTAPTAIRAIKKEDP G FIK +DLS LKYQFLAGERCD ATL WTE+ LKVPVIDHWWQTESGWPM+ANMVGV L+ VK GS+S PVCGYDI+IL+E GEE  + VEGYV+VKLPLPPGTL NLWGNPERFK GYL+ FPG+Y SGDGGYKDEDGYVFITGRVDD+INVAGHRLSTA+MEEIVASHKAVAECAVFG+HCE+KGQKPL
Sbjct:    1 MDYQEFYQKSIQQPEQFWNEQAKNIDWFKAPETILSKDKQDYNQWFEDGELNLSYLCIDKHIKDGFGSQNAIIFDSPVTNTKQHITFNQLHHEVSKLAGGLQVLGLQKGDTCIIYMPMIPQALYAMLACARIGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGIEIERIIPYKPFVDEAIAKAENKPEHVIVFDRLLGVEIPKKNYDIDYTTLVEKSPSIEAVSVESTHPSYILYTSGTTGTPKGIIRDSGGYATALKFSMKYIYGVDEGDTFWAASDVGWVVGHSYIVYGPLLNRNTTIVFEGKPIRTPDASTFWRVISEHRVKVMFTAPTAIRAIKKEDPEGKFIKHFDLSCLKYQFLAGERCDVATLTWTEEHLKVPVIDHWWQTESGWPMIANMVGVHLQEVKPGSSSFPVCGYDIQILNEEGEEVASSVEGYVAVKLPLPPGTLTNLWGNPERFKAGYLDRFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVASHKAVAECAVFGVHCELKGQKPL 538          

HSP 2 Score: 296.975 bits (759), Expect = 8.035e-85
Identity = 157/398 (39.45%), Postives = 237/398 (59.55%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTE--LVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPI--PFSTPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPWPSMART-IWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            +++  P +ILYTSG+TGTPKG++  + GY     ++ K ++   E D +W  +D+GW+ GHSYIVYGPL+N  TT++FEG P   PD   FW V+ +H++   +TAPTAIRA+ K+  E   +  +DLS +K     GE  +     W  E++   K P++D WWQTE+G  MI  +        KP  ++ P  G    +++E G+E+  + V+G + +K P P    T +WGN +R+K  Y   +   YF+GDG  +DE GY  ITGRVDDVI V+GH L TA +E+ +  H+AV+E A+ G   ++KG    G + LK +GE+     ++KEI Q +   IG +A    +   + LPKTRSGKI+R++LR IA  +  N+   ST+ +  ++ EI
Sbjct:  228 VESTHPSYILYTSGTTGTPKGIIRDSGGYATALKFSMKYIYGVDEGDTFWAASDVGWVVGHSYIVYGPLLNRNTTIVFEGKPIRTPDASTFWRVISEHRVKVMFTAPTAIRAIKKEDPEGKFIKHFDLSCLKYQFLAGERCDVATLTWTEEHL---KVPVIDHWWQTESGWPMIANMVGVHLQEVKPGSSSFPVCGYDIQILNEEGEEV-ASSVEGYVAVKLPLPPGTLTNLWGNPERFKAGYLDRFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVASHKAVAECAVFGVHCELKGQKPLGLVVLK-SGETSAEIQIKKEIVQDVRREIGAVASFRDVLVVNRLPKTRSGKILRKLLRNIADEQQYNI--PSTIDDVHIINEI 618          
BLAST of acyl- synthetase vs. nr
Match: gi|749648850|ref|WP_040249436.1| (propionyl-CoA synthetase [Psychroserpens mesophilus])

HSP 1 Score: 916.761 bits (2368), Expect = 0.000e+0
Identity = 427/538 (79.37%), Postives = 480/538 (89.22%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  YQ SI+NPE FW+ QS+ L W+TPPK IL KD+Y+Y QWF DG+LN+SYLCIDKHI+DGFG+QNAIIYDSPVT+ K++I++N+L+ EV++LAGGLQ +GL  GD  IIYMPMIPQALYAMLACARLGIIHSVVFGGFAP+ELAIRIDDCKPKA+ITAS+GIEIERIIPYKPFVD+AI+ + +KP+HVIVFDR+ GV+IP+K YDIDYT LV KS +V  V++ S HP YILYTSGTTG PKGIIRD+GGYATALKFSMKYIYGVDEGD FWAASDVGWVVGHSYI YGPLL+RNTTILFEGKPIKTPDASTFWRVISEH VKVMFTAPTAIRAIKKEDPNGD +K++D+S LKYQFLAGERCD ATL WT Q L VPVIDHWWQTESGWPMLANMVG+EL+ VK GSA  PVCGYDI+IL+E+GE      EGYV+VKLPLPPGTL NLWGNPERFK GYLN F G+Y SGDGGY+DEDGYVFITGRVDDIINVAGHRLSTA+MEE+VASH +VAECAVFGIHC++KGQKPL
Sbjct:    1 MNYQEFYQKSIQNPELFWKDQSEALEWFTPPKTILSKDKYDYYQWFEDGELNLSYLCIDKHIKDGFGEQNAIIYDSPVTNVKQYISFNKLHLEVSKLAGGLQSMGLKTGDVCIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPHELAIRIDDCKPKAIITASSGIEIERIIPYKPFVDEAILKSHYKPEHVIVFDRQLGVEIPNKSYDIDYTTLVEKSPSVEAVSLKSTHPSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYIYGVDEGDTFWAASDVGWVVGHSYIVYGPLLHRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPNGDLLKQFDMSCLKYQFLAGERCDVATLTWTRQHLNVPVIDHWWQTESGWPMLANMVGIELQSVKPGSAGFPVCGYDIRILNEDGEIAANGAEGYVAVKLPLPPGTLSNLWGNPERFKSGYLNRFDGYYFSGDGGYQDEDGYVFITGRVDDIINVAGHRLSTAEMEEVVASHNSVAECAVFGIHCDLKGQKPL 538          

HSP 2 Score: 286.96 bits (733), Expect = 3.351e-81
Identity = 151/398 (37.94%), Postives = 233/398 (58.54%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPF--STPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            + +  P +ILYTSG+TGTPKG++  T GY     ++ K ++   E D +W  +D+GW+ GHSYIVYGPL++  TT++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+    +L+ ++D+S +K     GE  +     W  +++     P++D WWQTE+G  M+  +        KP  A  P  G    +++E+G EI  N  +G + +K P  P     +WGN +R+K  Y + ++  YF+GDG  +DE GY  ITGRVDD+I V+GH L TA +E+ +  H +V+E A+ G   D+KG    G I LK +G +    ++++ I Q +   IG +A    +   + LPKTRSGKI+R++LR IA  +  N+   ST+ +  ++ EI
Sbjct:  228 LKSTHPSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYIYGVDEGDTFWAASDVGWVVGHSYIVYGPLLHRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPNGDLLKQFDMSCLKYQFLAGERCDVATLTWTRQHL---NVPVIDHWWQTESGWPMLANMVGIELQSVKPGSAGFPVCGYDIRILNEDG-EIAANGAEGYVAVKLPLPPGTLSNLWGNPERFKSGYLNRFDGYYFSGDGGYQDEDGYVFITGRVDDIINVAGHRLSTAEMEEVVASHNSVAECAVFGIHCDLKGQKPLGLIVLK-SGVTSTEKDIQQSIVQSVRHEIGAVASFRDVLVVNRLPKTRSGKILRKLLRNIADEQQYNI--PSTIDDVTIINEI 618          
BLAST of acyl- synthetase vs. nr
Match: gi|1363478209|gb|PSG85952.1| (propionyl-CoA synthetase [Mesoflavibacter sp. HG37] >gi|1363485934|gb|PSG93620.1| propionyl-CoA synthetase [Mesoflavibacter sp. HG96])

HSP 1 Score: 915.22 bits (2364), Expect = 0.000e+0
Identity = 428/538 (79.55%), Postives = 480/538 (89.22%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  Y  SI NPE FW+ QSQNL W+  P  IL KD+++Y QWFADGQLN+SYLCIDKHI DGFGDQNAIIYDSPVT+TK+HIT+NQL+ EV++LAGGLQ LGL KGDT IIYMPMIPQA+YAMLACAR+G+IHSVVFGGFAP+ELAIRIDDCKPKAVITAS+GIEI RIIPYKPFVD+AI  A+ KP+ VIVFDR++ V+IP+KPYD+DY  LV  S ++  + + + HP YILYTSGTTGKPKGIIRD+GGYATALKFSMK++YGV+EGDV+WAASDVGWVVGHSYI YGPLLNRNTTILFEGKPIKTPDASTFWRVISEH VKVMFTAPTAIRAIKKEDP GD IK YDLS L+YQFLAGERCD ATL+WTEQ+LKVPVIDHWWQTESGWPMLANMVGV+L+ VK GSASLPVCGYDI+IL+E G+E +  VEG V+ KLPLPPGTLLNLW NP+RFK GYL  FPG+Y SGDGGYKDEDGYVFITGRVDDIINVAGHRLSTA+MEE+VAS+ +VAECAVFG+HCEIKGQKPL
Sbjct:    1 MDYQKFYNSSIDNPEDFWKTQSQNLDWFKQPNTILSKDKHDYYQWFADGQLNLSYLCIDKHINDGFGDQNAIIYDSPVTNTKKHITFNQLHKEVSKLAGGLQALGLKKGDTCIIYMPMIPQAIYAMLACARIGVIHSVVFGGFAPHELAIRIDDCKPKAVITASHGIEINRIIPYKPFVDEAIKQAKSKPEKVIVFDREQDVEIPNKPYDVDYKTLVENSDSIDAIPLEATHPSYILYTSGTTGKPKGIIRDTGGYATALKFSMKHVYGVEEGDVYWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPEGDLIKNYDLSCLRYQFLAGERCDVATLHWTEQKLKVPVIDHWWQTESGWPMLANMVGVQLQDVKPGSASLPVCGYDIQILNEEGQEVEAGVEGLVTAKLPLPPGTLLNLWENPQRFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTAEMEEVVASNASVAECAVFGVHCEIKGQKPL 538          

HSP 2 Score: 299.286 bits (765), Expect = 1.182e-85
Identity = 157/386 (40.67%), Postives = 230/386 (59.59%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGTE--LVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDT 1007
            ++A  P +ILYTSG+TG PKG++  T GY     ++ K+V+  +E DVYW  +D+GW+ GHSYIVYGPL+N  TT++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+  E  L+  YDLS ++     GE  +    HW  +   K K P++D WWQTE+G  M+  +        KP  A+LP  G    +++E GQE++   V+G +  K P  P     +W N QR+K  Y   +   YF+GDG  +DE GY  ITGRVDD+I V+GH L TA +E+ +  + +V+E A+ G   +IKG    G + LK +G + D++ ++ EI + +   IG +A    +   + LPKTRSGKI+R++LR IA  +  N+  T
Sbjct:  228 LEATHPSYILYTSGTTGKPKGIIRDTGGYATALKFSMKHVYGVEEGDVYWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPEGDLIKNYDLSCLRYQFLAGERCDVATLHWTEQ---KLKVPVIDHWWQTESGWPMLANMVGVQLQDVKPGSASLPVCGYDIQILNEEGQEVEAG-VEGLVTAKLPLPPGTLLNLWENPQRFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTAEMEEVVASNASVAECAVFGVHCEIKGQKPLGLVVLK-SGATTDYEAIQTEIIKDVRKEIGAVASFKDVLVVNRLPKTRSGKILRKLLRNIADEQQYNIPST 608          
BLAST of acyl- synthetase vs. nr
Match: gi|1086475560|emb|SDE68264.1| (propionyl-CoA synthetase [Ulvibacter litoralis])

HSP 1 Score: 914.835 bits (2363), Expect = 0.000e+0
Identity = 425/538 (79.00%), Postives = 483/538 (89.78%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQN Y  SI +P+ FW +Q++ L WYT PK  + KDEY+Y QWF DG+LN+SYLCID HI +G+G+QNAIIYDSPVT TK HIT+NQL+ EV++LAGGLQDLGL KGDT IIYMPMIPQALYAMLACAR+G+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNGIEI+RIIPYKPFVD+AI  +++KP+HVIVFDRK GV+IP K YDIDYT LV KS+++  V+++++HP YILYTSGTTG PKGIIRD+GGYATALKFSMKYIYGV+EGD FWAASDVGWVVGHS+IAYGPLLNRNTT+LFEGKPI+TPDASTFWRVISEH+VKVMFTAPTAIRAIKKEDPNGD +K++DLS LKYQFLAGERCD ATL WTE++L VPVIDHWWQTESGWPM+ANMVGV+L  VK GS+  PVCGYDI+IL+E G+E   DVEGYV+VKLPLPPGTL NLWGNP+RF  GYL  FPG+Y SGDGGYKDEDGYVFITGRVDD+INVAGHRLSTA+MEEIVASHKAVAECAVFGIHC +KGQKPL
Sbjct:    1 MKYQNFYSRSIDHPDEFWLEQARQLNWYTQPKIGVSKDEYDYDQWFEDGELNLSYLCIDTHIANGYGNQNAIIYDSPVTTTKEHITFNQLHAEVSKLAGGLQDLGLQKGDTCIIYMPMIPQALYAMLACARIGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGIEIDRIIPYKPFVDEAIQKSKNKPEHVIVFDRKLGVEIPKKNYDIDYTTLVEKSSSIEAVSLSASHPSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYIYGVEEGDTFWAASDVGWVVGHSFIAYGPLLNRNTTVLFEGKPIRTPDASTFWRVISEHQVKVMFTAPTAIRAIKKEDPNGDMLKQFDLSCLKYQFLAGERCDVATLTWTEEKLNVPVIDHWWQTESGWPMIANMVGVQLNLVKPGSSGFPVCGYDIRILNEEGQEVADDVEGYVTVKLPLPPGTLRNLWGNPKRFVAGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVASHKAVAECAVFGIHCNLKGQKPL 538          

HSP 2 Score: 287.345 bits (734), Expect = 2.242e-81
Identity = 150/376 (39.89%), Postives = 219/376 (58.24%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIA 997
            + A  P +ILYTSG+TGTPKG++  T GY     ++ K ++  +E D +W  +D+GW+ GHS+I YGPL+N  TTV+FEG P   PD   FW V+ +H++   +TAPTAIRA+ K+    +++ ++DLS +K     GE  +     W  E   K   P++D WWQTE+G  MI  +        KP  +  P  G    +++E GQE+  + V+G + +K P  P   R +WGN +R+   Y   +   YF+GDG  +DE GY  ITGRVDDVI V+GH L TA +E+ +  H+AV+E A+ G   ++KG    G I LK +G++     +  EI   +   IG +A    +     LPKTRSGKI+R++LR IA
Sbjct:  228 LSASHPSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYIYGVEEGDTFWAASDVGWVVGHSFIAYGPLLNRNTTVLFEGKPIRTPDASTFWRVISEHQVKVMFTAPTAIRAIKKEDPNGDMLKQFDLSCLKYQFLAGERCDVATLTWTEE---KLNVPVIDHWWQTESGWPMIANMVGVQLNLVKPGSSGFPVCGYDIRILNEEGQEV-ADDVEGYVTVKLPLPPGTLRNLWGNPKRFVAGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVASHKAVAECAVFGIHCNLKGQKPLGLIVLK-SGDTFKEQEIASEIISQVRKEIGAVASFRDVLIVERLPKTRSGKILRKLLRNIA 598          
BLAST of acyl- synthetase vs. nr
Match: gi|518217021|ref|WP_019387229.1| (propionyl-CoA synthetase [Arenitalea lutea] >gi|1109869712|emb|SHI56328.1| propionyl-CoA synthetase [Arenitalea lutea])

HSP 1 Score: 914.835 bits (2363), Expect = 0.000e+0
Identity = 426/538 (79.18%), Postives = 480/538 (89.22%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  YQ+S+K+PE FW +Q+Q L W+  PK IL KD+++Y QWF DGQLN+SYLCIDKHI+DGFG+Q+AIIYDSPVT+ K+HI++NQL+ EV++LAGGLQ LGL +GDT IIYMPMIPQA+YAMLAC R+G+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNGIEIE+IIPYKPFVD+AI  A++KP+HV+VFDR+ GV+IP K YDIDY +LV  S +V  V+V S HP YILYTSGTTG PKGIIRD+GGYATALKFSMKY+YGVDEGD FWAASDVGWVVGHSYI YGPLLNRNTTILFEGKPI+TPDASTFWRVISEH VKVMFTAPTAIRAIKKEDP G  IK++D+S LKYQFLAGERCD ATLNWT ++L VPVIDHWWQTESGWPMLANMVGVEL+ VK GSAS PVCGYDIKIL+E GEE +  VEGYV+VKLPLPPGTL NLWGNP+RFK GYL  FPG+Y SGDGGYKDED YVFITGRVDDIINVAGHRLSTA+MEEIVAS+K VAECAVFG+HCEIKGQKPL
Sbjct:    1 MNYQEFYQNSLKHPEQFWSQQAQELEWFKSPKIILSKDKHDYFQWFEDGQLNLSYLCIDKHIKDGFGEQDAIIYDSPVTNAKKHISFNQLHFEVSKLAGGLQSLGLERGDTCIIYMPMIPQAIYAMLACVRIGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGIEIEKIIPYKPFVDEAITKAENKPEHVVVFDRQLGVEIPKKNYDIDYKSLVENSPSVDAVSVESIHPSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYVYGVDEGDTFWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIRTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPTGHMIKRFDMSCLKYQFLAGERCDVATLNWTHEKLNVPVIDHWWQTESGWPMLANMVGVELQEVKPGSASFPVCGYDIKILNEAGEEVEASVEGYVAVKLPLPPGTLSNLWGNPKRFKSGYLERFPGYYFSGDGGYKDEDDYVFITGRVDDIINVAGHRLSTAEMEEIVASNKVVAECAVFGVHCEIKGQKPL 538          

HSP 2 Score: 288.115 bits (736), Expect = 1.339e-81
Identity = 153/393 (38.93%), Postives = 232/393 (59.03%), Query Frame = 0
Query:  633 PLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQGT--ELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            P +ILYTSG+TGTPKG++  T GY     ++ K V+   E D +W  +D+GW+ GHSYIVYGPL+N  TT++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+     ++ ++D+S +K     GE  +    +W +E   K   P++D WWQTE+G  M+  +        KP  A+ P  G    +++E G+E++ + V+G + +K P  P     +WGN +R+K  Y   +   YF+GDG  +DE  Y  ITGRVDD+I V+GH L TA +E+ +  ++ V+E A+ G   +IKG    G I LK   +S D+  ++KEI + + + IG +A    +   + LPKTRSGKI+R++LR IA     N+   ST+ +  ++ EI
Sbjct:  233 PSYILYTSGTTGTPKGIIRDTGGYATALKFSMKYVYGVDEGDTFWAASDVGWVVGHSYIVYGPLLNRNTTILFEGKPIRTPDASTFWRVISEHNVKVMFTAPTAIRAIKKEDPTGHMIKRFDMSCLKYQFLAGERCDVATLNWTHE---KLNVPVIDHWWQTESGWPMLANMVGVELQEVKPGSASFPVCGYDIKILNEAGEEVEAS-VEGYVAVKLPLPPGTLSNLWGNPKRFKSGYLERFPGYYFSGDGGYKDEDDYVFITGRVDDIINVAGHRLSTAEMEEIVASNKVVAECAVFGVHCEIKGQKPLGLIVLKSNVDS-DNSQIQKEIIKDVRNEIGAVASFKDVLIVNRLPKTRSGKILRKLLRNIADERQYNI--PSTIDDVAIINEI 618          
BLAST of acyl- synthetase vs. nr
Match: gi|1306875354|ref|WP_101018935.1| (propionyl-CoA synthetase [Olleya sp. Bg11-27] >gi|1304122916|gb|AUC76839.1| propionyl-CoA synthetase [Olleya sp. Bg11-27])

HSP 1 Score: 913.294 bits (2359), Expect = 0.000e+0
Identity = 421/538 (78.25%), Postives = 478/538 (88.85%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  Y+ SI  P  FW++Q+  L W+  PK IL KD+Y+Y QWF DGQLN+SYLCIDKHI++GFGDQNAIIYDSPVT+ K+HIT+NQL+ EV++LAGGLQ LGL KGDT IIYMPMIPQALYAMLACAR+G+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNG+EI++IIPYKPFVD AI  A++KP+HVIVFDR  GV+IP K YDIDYT LV  S +++ VA+ S HP YILYTSGTTG PKGIIRD+GGYATALKF+MKYIYGV+EG+ FWAASDVGWVVGHS+IAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEH VK MFTAPTAIRAI+KEDPNG  +K++DLS LKYQFLAGERCD ATL W E++L VPVIDHWWQTESGWPM++NMVGV L+ +K GSASLPV GYDI+IL+E GEE Q+ VEGYV+VKLPLPPGTL NLWGNPERFK GYL  FPG+Y SGDGGYKDEDGYVFITGRVDD+INVAGHRLSTA+MEEIV+SHK+VAECAVFG+HCE+KGQKPL
Sbjct:    1 MYYQEFYRKSIDQPAQFWQEQAMQLEWFKVPKTILSKDKYDYDQWFEDGQLNLSYLCIDKHIKEGFGDQNAIIYDSPVTNVKQHITFNQLHHEVSKLAGGLQSLGLQKGDTCIIYMPMIPQALYAMLACARIGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGVEIQKIIPYKPFVDQAITKAKNKPEHVIVFDRALGVEIPKKSYDIDYTTLVEASPSIAAVAIESTHPSYILYTSGTTGTPKGIIRDTGGYATALKFAMKYIYGVNEGETFWAASDVGWVVGHSFIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKAMFTAPTAIRAIRKEDPNGHMLKRFDLSCLKYQFLAGERCDVATLKWAEEKLNVPVIDHWWQTESGWPMISNMVGVGLQEIKPGSASLPVSGYDIQILNEEGEEVQSSVEGYVTVKLPLPPGTLSNLWGNPERFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVSSHKSVAECAVFGVHCELKGQKPL 538          

HSP 2 Score: 294.278 bits (752), Expect = 6.516e-84
Identity = 154/398 (38.69%), Postives = 237/398 (59.55%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            +++  P +ILYTSG+TGTPKG++  T GY     +  K ++   E + +W  +D+GW+ GHS+I YGPL+N  TT++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+     ++ ++DLS +K     GE  +     W  E   K   P++D WWQTE+G  MI+ +        KP  A+LP  G    +++E G+E++ + V+G + +K P  P     +WGN +R+K  Y   +   YF+GDG  +DE GY  ITGRVDDVI V+GH L TA +E+ ++ H++V+E A+ G   ++KG    G + LK T  + D+D ++ +I Q +   IG +A   ++   S LPKTRSGKI+R++LR IA  +  N+   ST+ +  ++ EI
Sbjct:  228 IESTHPSYILYTSGTTGTPKGIIRDTGGYATALKFAMKYIYGVNEGETFWAASDVGWVVGHSFIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHNVKAMFTAPTAIRAIRKEDPNGHMLKRFDLSCLKYQFLAGERCDVATLKWAEE---KLNVPVIDHWWQTESGWPMISNMVGVGLQEIKPGSASLPVSGYDIQILNEEGEEVQSS-VEGYVTVKLPLPPGTLSNLWGNPERFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVSSHKSVAECAVFGVHCELKGQKPLGLVVLK-TETALDNDEIQNQIIQAVRQEIGAVASFREVLVVSRLPKTRSGKILRKLLRNIADEQQYNI--PSTIDDVTIIDEI 618          
BLAST of acyl- synthetase vs. nr
Match: gi|1222793596|ref|WP_090838685.1| (propionyl-CoA synthetase [Olleya namhaensis] >gi|1097364952|emb|SFI96595.1| propionyl-CoA synthetase [Olleya namhaensis])

HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0
Identity = 420/538 (78.07%), Postives = 478/538 (88.85%), Query Frame = 0
Query:    1 MTYQNIYQDSIKNPESFWEKQSQNLAWYTPPKHILGKDEYNYPQWFADGQLNVSYLCIDKHIEDGFGDQNAIIYDSPVTHTKRHITYNQLYDEVARLAGGLQDLGLSKGDTVIIYMPMIPQALYAMLACARLGIIHSVVFGGFAPNELAIRIDDCKPKAVITASNGIEIERIIPYKPFVDDAIILAQHKPKHVIVFDRKKGVDIPHKPYDIDYTALVNKSTAVSPVAVASNHPLYILYTSGTTGKPKGIIRDSGGYATALKFSMKYIYGVDEGDVFWAASDVGWVVGHSYIAYGPLLNRNTTILFEGKPIKTPDASTFWRVISEHKVKVMFTAPTAIRAIKKEDPNGDFIKKYDLSSLKYQFLAGERCDAATLNWTEQQLKVPVIDHWWQTESGWPMLANMVGVELKPVKAGSASLPVCGYDIKILDENGEETQTDVEGYVSVKLPLPPGTLLNLWGNPERFKDGYLNTFPGFYSSGDGGYKDEDGYVFITGRVDDIINVAGHRLSTADMEEIVASHKAVAECAVFGIHCEIKGQKPL 538
            M YQ  Y+ SI  P  FW++Q+  L W+  PK IL KD+Y+Y QWF DGQLN+SYLCIDKHI++GFGDQNAIIYDSPVT+ K+HIT+NQL+ EV++LAGGLQ LGL KGDT IIYMPMIPQALYAMLACAR+G+IHSVVFGGFAP+ELAIRIDDCKPKA+ITASNG+EI++IIPYKPFVD AI  A++KP+HVIVFDR  GV+IP K YDIDYT LV  S +++ VA+ S HP YILYTSGTTG PKGIIRD+GGYATALKF+MKYIYGV+EG+ FWAASDVGWVVGHS+IAYGPLLNRNT+ILFEGKPIKTPDASTFWRVISEH VK MFTAPTAIRAI+KEDPNG  +K++DLS LKYQFLAGERCD ATL W E++L VPVIDHWWQTESGWPM++NMVGV L+ +K GSASLPV GYDI+IL+E GEE Q+ VEGYV+VKLPLPPGTL NLWGNPERFK GYL  FPG+Y SGDGGYKDEDGYVFITGRVDD+INVAGHRLSTA+MEEIV+SHK+VAECAVFG+HCE+KGQKPL
Sbjct:    1 MYYQEFYRKSIDQPTQFWQEQAMQLEWFKVPKTILSKDKYDYDQWFEDGQLNLSYLCIDKHIKEGFGDQNAIIYDSPVTNVKQHITFNQLHHEVSKLAGGLQSLGLQKGDTCIIYMPMIPQALYAMLACARIGVIHSVVFGGFAPHELAIRIDDCKPKAIITASNGVEIQKIIPYKPFVDQAITKAKNKPEHVIVFDRALGVEIPKKSYDIDYTTLVEASPSIAAVAIESTHPSYILYTSGTTGTPKGIIRDTGGYATALKFAMKYIYGVNEGETFWAASDVGWVVGHSFIAYGPLLNRNTSILFEGKPIKTPDASTFWRVISEHNVKAMFTAPTAIRAIRKEDPNGHMLKRFDLSCLKYQFLAGERCDVATLKWAEEKLNVPVIDHWWQTESGWPMISNMVGVGLQEIKPGSASLPVSGYDIQILNEEGEEVQSSVEGYVTVKLPLPPGTLSNLWGNPERFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVSSHKSVAECAVFGVHCELKGQKPL 538          

HSP 2 Score: 291.967 bits (746), Expect = 6.134e-83
Identity = 152/398 (38.19%), Postives = 237/398 (59.55%), Query Frame = 0
Query:  628 MDAEDPLFILYTSGSTGTPKGMLHTTAGYMVYTAYTFKNVFQYQEDDVYWCTADIGWITGHSYIVYGPLINGATTVMFEGVP-SYPDFGRFWDVVQKHKITQFYTAPTAIRALAKQ--GTELVDKYDLSSIKVLGSVGEPINEEAWHWYNENIGKKKSPIVDTWWQTETGGIMITPIPFS--TPTKPTYATLPFIGVQPTLMDENGQEIKGNQVDGRLCIKFPW-PSMARTIWGNHQRYKDTYFSAYENMYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGTAPIEDAINEHQAVSESAIVGFPHDIKGSALYGYITLKDTGESRDHDNMRKEINQLISDRIGPIAKLDKIQFTSGLPKTRSGKIMRRILRKIACNEIDNLGDTSTLLNPEVVQEI 1019
            +++  P +ILYTSG+TGTPKG++  T GY     +  K ++   E + +W  +D+GW+ GHS+I YGPL+N  T+++FEG P   PD   FW V+ +H +   +TAPTAIRA+ K+     ++ ++DLS +K     GE  +     W  E   K   P++D WWQTE+G  MI+ +        KP  A+LP  G    +++E G+E++ + V+G + +K P  P     +WGN +R+K  Y   +   YF+GDG  +DE GY  ITGRVDDVI V+GH L TA +E+ ++ H++V+E A+ G   ++KG    G + LK T  + D+D ++ +I Q +   IG +A   ++   + LPKTRSGKI+R++LR IA  +  N+   ST+ +  ++ EI
Sbjct:  228 IESTHPSYILYTSGTTGTPKGIIRDTGGYATALKFAMKYIYGVNEGETFWAASDVGWVVGHSFIAYGPLLNRNTSILFEGKPIKTPDASTFWRVISEHNVKAMFTAPTAIRAIRKEDPNGHMLKRFDLSCLKYQFLAGERCDVATLKWAEE---KLNVPVIDHWWQTESGWPMISNMVGVGLQEIKPGSASLPVSGYDIQILNEEGEEVQSS-VEGYVTVKLPLPPGTLSNLWGNPERFKSGYLERFPGYYFSGDGGYKDEDGYVFITGRVDDVINVAGHRLSTAEMEEIVSSHKSVAECAVFGVHCELKGQKPLGLVVLK-TETALDNDAIQNQIIQAVRQEIGAVASFREVLVVNRLPKTRSGKILRKLLRNIADEQQYNI--PSTIDDVTIIDEI 618          
The following BLAST results are available for this feature:
BLAST of acyl- synthetase vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 9
Match NameE-valueIdentityDescription
EMLSAG000000045072.803e-16245.82supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-... [more]
EMLSAG000000069464.561e-15545.12supercontig:LSalAtl2s:LSalAtl2s393:601207:603901:-... [more]
EMLSAG000000065771.689e-13655.46supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-... [more]
EMLSAG000000068453.653e-2323.27supercontig:LSalAtl2s:LSalAtl2s387:339966:342548:1... [more]
EMLSAG000000063092.346e-1726.69supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-... [more]
EMLSAG000000128911.445e-1122.93supercontig:LSalAtl2s:LSalAtl2s981:104426:107346:-... [more]
EMLSAG000000017092.904e-1122.19supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1... [more]
EMLSAG000000099041.852e-924.10supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-... [more]
EMLSAG000000020814.471e-720.36supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1... [more]
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BLAST of acyl- synthetase vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|12231045|sp|P28812.2|Y3568_PSEAE8.665e-8754.83RecName: Full=Uncharacterized protein PA3568[more]
gi|75061996|sp|Q5REB8.1|ACSS3_PONAB1.011e-7150.89RecName: Full=Acyl-CoA synthetase short-chain fami... [more]
gi|74752698|sp|Q9H6R3.1|ACSS3_HUMAN1.195e-7150.54RecName: Full=Acyl-CoA synthetase short-chain fami... [more]
gi|171769795|sp|A7MB45.1|ACSS3_BOVIN2.086e-6850.89RecName: Full=Acyl-CoA synthetase short-chain fami... [more]
gi|172046857|sp|Q14DH7.2|ACSS3_MOUSE1.806e-7150.00RecName: Full=Acyl-CoA synthetase short-chain fami... [more]
gi|226709490|sp|B8FIN2.1|ACSA_DESAA3.562e-17957.99RecName: Full=Acetyl-coenzyme A synthetase; Short=... [more]
gi|226709500|sp|A0LG91.1|ACSA_SYNFM3.806e-17962.32RecName: Full=Acetyl-coenzyme A synthetase; Short=... [more]
gi|259710029|sp|B0S8X7.1|ACSA_LEPBA6.692e-17860.14RecName: Full=Acetyl-coenzyme A synthetase; Short=... [more]
gi|166919472|sp|A0L576.1|ACSA_MAGMM9.958e-17759.72RecName: Full=Acetyl-coenzyme A synthetase; Short=... [more]
gi|122312288|sp|Q0AAW0.1|ACSA_ALKEH2.401e-17660.66RecName: Full=Acetyl-coenzyme A synthetase; Short=... [more]

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BLAST of acyl- synthetase vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1140751131|ref|WP_076615732.1|4.004e-8580.68propionyl-CoA synthetase [Winogradskyella sp. J14-... [more]
gi|1317348621|ref|WP_101449387.1|2.192e-8579.93propionyl-CoA synthetase [Aquimarina sp. MAR_2010_... [more]
gi|1236591505|ref|WP_094968451.1|3.817e-8681.97acetate--CoA ligase [Winogradskyella sp. WNB302] >... [more]
gi|1306818290|ref|WP_100996365.1|8.035e-8580.48propionyl-CoA synthetase [Lacinutrix sp. Bg11-31] ... [more]
gi|749648850|ref|WP_040249436.1|3.351e-8179.37propionyl-CoA synthetase [Psychroserpens mesophilu... [more]
gi|1363478209|gb|PSG85952.1|1.182e-8579.55propionyl-CoA synthetase [Mesoflavibacter sp. HG37... [more]
gi|1086475560|emb|SDE68264.1|2.242e-8179.00propionyl-CoA synthetase [Ulvibacter litoralis][more]
gi|518217021|ref|WP_019387229.1|1.339e-8179.18propionyl-CoA synthetase [Arenitalea lutea] >gi|11... [more]
gi|1306875354|ref|WP_101018935.1|6.516e-8478.25propionyl-CoA synthetase [Olleya sp. Bg11-27] >gi|... [more]
gi|1222793596|ref|WP_090838685.1|6.134e-8378.07propionyl-CoA synthetase [Olleya namhaensis] >gi|1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold2333_size17043supercontigscaffold2333_size17043:6721..10637 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
Note-
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
maker-scaffold2333_size17043-snap-gene-0.0-mRNA-1maker-scaffold2333_size17043-snap-gene-0.0-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold2333_size17043:6721..10637+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>maker-scaffold2333_size17043-snap-gene-0.0 ID=maker-scaffold2333_size17043-snap-gene-0.0|Name=acyl- synthetase|organism=Tigriopus kingsejongensis|type=gene|length=3917bp|location=Sequence derived from alignment at scaffold2333_size17043:6721..10637+ (Tigriopus kingsejongensis)
ATGACCTATCAAAACATTTACCAAGATAGTATCAAAAATCCAGAATCTTT CTGGGAAAAACAATCACAAAATTTAGCCTGGTACACGCCGCCTAAACATA TTTTGGGTAAGGATGAATATAATTATCCGCAGTGGTTTGCAGACGGGCAA CTCAATGTTAGCTACCTATGTATCGACAAGCATATTGAAGATGGTTTTGG TGACCAAAATGCTATTATTTATGATTCGCCTGTAACGCATACAAAACGTC ATATTACTTACAATCAACTCTACGATGAAGTGGCGCGTTTAGCCGGTGGA TTACAAGATTTGGGACTATCAAAAGGCGATACTGTAATTATATACATGCC AATGATTCCGCAGGCTTTATACGCAATGTTAGCTTGCGCGCGTTTAGGCA TTATACACTCCGTTGTGTTTGGTGGTTTTGCGCCCAATGAGTTGGCAATA CGCATTGACGATTGCAAACCAAAAGCAGTAATTACAGCATCAAATGGTAT TGAAATAGAACGTATTATACCTTACAAACCTTTTGTAGATGACGCCATTA TACTAGCACAACACAAACCTAAACACGTTATTGTTTTTGACCGAAAAAAA GGAGTTGATATCCCACATAAACCGTACGATATTGATTATACAGCTTTGGT AAATAAGTCTACAGCAGTCTCTCCAGTTGCAGTGGCATCTAACCATCCAT TATATATATTATATACCTCTGGTACAACAGGAAAACCAAAGGGTATTATT AGAGATTCTGGAGGTTATGCAACTGCACTAAAATTTTCGATGAAATATAT TTATGGCGTTGATGAAGGTGACGTGTTTTGGGCTGCAAGTGATGTAGGTT GGGTTGTAGGACACAGTTATATTGCCTATGGTCCGTTACTTAACAGAAAT ACAACTATTTTATTTGAAGGCAAACCTATTAAAACACCAGATGCATCCAC ATTTTGGCGTGTTATAAGCGAGCATAAAGTAAAAGTTATGTTTACTGCAC CAACTGCTATTAGGGCCATAAAAAAGGAAGATCCTAATGGTGATTTTATA AAAAAATACGATTTAAGCAGTCTTAAATATCAGTTTTTAGCAGGTGAGCG TTGTGACGCAGCAACTTTAAATTGGACAGAACAACAGCTAAAAGTACCAG TAATAGACCATTGGTGGCAAACCGAGAGTGGTTGGCCAATGCTTGCAAAT ATGGTTGGTGTTGAGCTAAAACCAGTAAAAGCAGGCTCGGCATCTTTACC CGTTTGTGGCTATGATATTAAAATTTTGGATGAAAACGGTGAAGAAACAC AAACTGATGTTGAAGGTTATGTTAGTGTAAAACTGCCGTTACCACCAGGA ACATTGTTAAATTTATGGGGAAATCCTGAACGTTTTAAAGATGGGTATCT TAATACCTTTCCTGGATTTTATTCTTCAGGAGATGGCGGATACAAGGACG AAGATGGTTATGTGTTTATTACAGGTCGTGTAGATGATATTATAAACGTT GCAGGCCATAGATTATCCACCGCTGATATGGAAGAAATTGTAGCATCACA CAAAGCTGTTGCCGAATGTGCTGTTTTTGGTATCCATTGCGAAATAAAAG GGCAGAAACCTTTAGGTTTGGTCGTTTTAAAAAATGATCATAATTTAGAC CACGCTTTAATTTCAAAAGAAATAATAAACGATGTACGCAAAGCTATTGG TGCAGTTGCAGCGCTTAGAAATGTGCTTGTTGTAAAACGCTTGCCTAAAA CACGTTCAGGAAAAATTCTTCGTAAATTATTACGAGATATTTCAGATAAT TTTGAATATAATATCCCATCAACCATTGACGATATTGAAATAATTTCAGA AATTAAAACCGTTTATAAAAACAATCAAATAGGAATCCATTCAGTTTAAC AAATAACTTTTTAAACAAAAAACGATACTTAAACTCTTAAATGAAATCAA ATTTTAGATTATCCCTTTCAGGGGACTTTAGGGGTGTTTTCAAATAAAAA CTAAAAAAAAACTATACAATGAGTAATTATCACATAAAACATTTTGAAGA ATACTTTCAAGTTTATAGAAAATCTGTTAGAAACCCTGAGGCCTTTTGGG AAGAAATAGCCGAAGAACATTTTGCGTGGCGAAAAAAATGGGATAACGTT TTAAGTTGGGATTTTAAAAAACCAGAAGGAAAATGGTTTGAAGGTGCCAA ACTCAATATTACCGAGAACTGTATTGATAGACATCTACGAACACGCGGTA ATAAAACAGCTATTTTATTTGAGCCAAACGACCCAAAGGAACCGGCAGAG CATATTACTTATAAACAGTTGCATGAGCGCGTTTGTCAGTTTGCTAACGT GCTAAAAAATAAAGGTATAAAAAAAGGAGACCGCGTTTGTATTTATTTGC CAATGATACCCGAATTGGCCATTTCAATTCTTGCATGTGCACGTATTGGG GCGATACATTCCGTTGTATTTGCAGGTTTTTCAGCAACAGCATTATCAAC ACGTATTAATGATTGTGACGCTAAAATGGTTATAACGTCTGATGGTTCAT ATCGTGGTGCTAAAACGTTAGATTTAAAAGGTATTGTAGACGAGGCTTTA GATATTTGTCCTGGTGTAACAACGGTTTTAGTAGCAAAACGTATTCATTC AGAAATTACAATGAAAGAAGGACGTGATGAGTGGTTGCAACCCCTTTTAG ACGAGGCCTATCACGACTGTGATATAGAAATTATGGATGCTGAAGATCCG CTATTTATATTATACACATCTGGCTCAACAGGTACACCTAAAGGCATGTT ACATACTACGGCAGGTTATATGGTGTACACGGCATACACCTTTAAAAACG TATTTCAATATCAGGAAGATGATGTGTATTGGTGTACCGCAGATATTGGG TGGATTACTGGCCACAGTTATATTGTATATGGTCCATTAATAAATGGCGC TACTACCGTAATGTTCGAAGGTGTACCGAGTTATCCAGATTTTGGACGTT TTTGGGATGTAGTACAAAAACACAAAATCACTCAATTTTACACCGCACCT ACAGCAATAAGAGCTTTAGCAAAACAAGGAACTGAGCTGGTTGATAAGTA TGATTTATCATCTATAAAAGTACTTGGATCCGTAGGCGAACCTATTAATG AAGAAGCTTGGCACTGGTATAACGAGAATATTGGCAAGAAAAAAAGTCCA ATAGTAGATACGTGGTGGCAAACTGAAACAGGTGGAATAATGATAACGCC AATTCCGTTTTCAACACCTACAAAACCTACATACGCAACGTTGCCGTTTA TAGGTGTTCAACCAACATTAATGGATGAAAATGGCCAGGAAATTAAAGGC AACCAAGTTGATGGGCGTTTGTGTATAAAATTTCCGTGGCCGTCAATGGC TAGAACTATTTGGGGAAATCACCAACGTTATAAAGACACCTATTTTTCTG CTTATGAAAATATGTATTTTACAGGTGATGGTGCTTTACGTGATGAAGTA GGGTATTACCGTATTACCGGACGTGTGGATGATGTAATTATTGTATCTGG GCATAACCTAGGAACTGCGCCAATTGAAGATGCTATTAATGAGCATCAAG CCGTTTCAGAGTCTGCTATTGTTGGTTTTCCACATGATATTAAAGGCAGT GCACTTTATGGATATATTACTTTGAAAGACACAGGCGAAAGCCGTGACCA TGACAATATGCGTAAAGAAATTAATCAACTAATTAGTGATAGAATTGGGC CTATTGCTAAACTCGATAAAATTCAATTTACAAGTGGTTTGCCAAAAACG CGCTCAGGAAAAATAATGCGTCGAATACTTCGGAAAATTGCGTGTAATGA AATAGATAATTTAGGAGATACCAGTACATTATTAAATCCGGAGGTGGTTC AGGAAATTATAGATAAT
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Synonyms
The feature 'acyl- synthetase' has the following synonyms
Synonym
Tk02004
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