BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. L. salmonis genes
Match: EMLSAG00000004676 (supercontig:LSalAtl2s:LSalAtl2s244:183507:188860:-1 gene:EMLSAG00000004676 transcript:EMLSAT00000004676 description:"maker-LSalAtl2s244-augustus-gene-1.13")

HSP 1 Score: 447.973 bits (1151), Expect = 4.039e-143
Identity = 267/674 (39.61%), Postives = 383/674 (56.82%), Query Frame = 0

Query:   93 LAARYGVGYTPCLVVVDAQDGSVITRDGGTAVTRQGHAAFASWEPPAQPDVDTSSVAALGANPDPWQRVVAQMLVDRVAQLLSG------SAAARSFGLCDPRWA----EASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLDEPA--PPPPATVTRPPVPIVPAPLPMGHLLTYMTNE--SEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHS-GYRAPTSQEQMDGASVVEGFVCQAC-EQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSR-DHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFESDALTGSKTLSLRVLM---WGELACRDFRLFATPTSES 743
            L +R  V   P LVVV+  DGSV++RD  + +T +G AAF+ W+      VDT+S+  L  N    ++ +     D + +LLS       +   RS  L +P+ +     A+G F++L  +GFE     L  P   S  +L AFR  ++ ++D       P   +T     IV A        +   NE  SEF QK++ E +    +E+   Q+ A   +P E LE +A        + H+  +  +++DLLL+ELL+WFKNDFF W ++P C  C      + G   PT  E  DGAS+VE + C  C   + RFPRYHS P KLLETRRGRCGEWANCFAL+ R M +D R VLDWTDHVWVE+FS+ ++RW+H DPCE   DKPLLYEVGW K+L+YVIATS  EV DVT RY +      +++R  V+E+WL++++  LT+ +     + +     +RRL +LV L+     P + D E L GRQSGS AW++ RGE+G    P     W+    E +++ F L YNV+ D Y R   G E + GW  G  E++ + R ++ D W+   LARKE +    + W +DLS+T LSV+ ++L I S T  +GRV+WQL G++  L+PS  +T  S+AL G+K L L   +    G++A +  +LF   T+ S
Sbjct:  206 LGSRLEVNGIPALVVVNLFDGSVMSRDARSEITSKGSAAFSIWDSMCSV-VDTTSLNLLKDN----EKDIMDSACDILNRLLSNVIKDPTNMKYRSIKLGNPKISSALLNANGAFEVLFTVGFEESDDCLILPMNASISILTAFRDKIKDMIDNKTNQEEPEIALT----SIVGAQSSSARNNSTFQNEKTSEFNQKILNEIELSKTYESSINQALATSCIPHESLEMKA----KEASKYHQYVNDKLMKDLLLMELLSWFKNDFFQWFDAPKCSNCNDQVTKTIGCVTPTDIELRDGASIVEKYECPLCFRSDFRFPRYHSNPSKLLETRRGRCGEWANCFALLARXMKYDVRRVLDWTDHVWVEIFSEHQDRWVHVDPCEGVLDKPLLYEVGWKKELSYVIATSIYEVIDVTPRYVKCIEETKKNKRYLVQEKWLSDTIGNLTDXLMNNLSKEQKELCVQRRLKDLVELMSQ-KNPINLD-ESLPGRQSGSSAWRLQRGEVGEQFKP---YVWKPNEHECETKEFELCYNVIEDTYIR---GKERIVGWRNGLSEMKDLFRKEERD-WNMVYLARKERLETGKLSWDIDLSETNLSVKCIELKIESKTYENGRVLWQLCGNNVCLIPSSSTTLNSEALAGAKKLXLTASLSGGKGDVAWQHAQLFRASTNCS 857          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|82192765|sp|Q503I8.1|NGLY1_DANRE (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 369.007 bits (946), Expect = 1.254e-116
Identity = 197/434 (45.39%), Postives = 266/434 (61.29%), Query Frame = 0

Query:  266 MTNESEFYQKMVAES-QHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLD-QPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEV--DSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQL 692
             T+ S  +Q+ V  + QHV+ +E+P+ Q +A   +P E L SRA  +L +   A   A  +   D+L+L+LL WFK+DFF WV++  C  CGG +  SG  +P+S +    A  VE   C  C+   RFPRY++ PEKLLETR+GRCGEWANCF L+CR++G + R++ D TDHVW EV+SQ++ RW+H DPCEN CDKPLLYEVGWGKKL+Y++A SKD+V DVT+RYS  H  + SRRT+V+E WL   L KL  + Q+     R  QL +R L+ELV  + P   QP    GE L GR SGS+AW+ ARGE G     +  +     P+E   +S+ FH+ YNV +D Y RVSNG E + GW  G    E M R ++ D W    LAR E      M WK + +   + ++S  + + S T  SG V W L
Sbjct:  150 FTSSSATFQRTVQSNFQHVLVYEDPELQQKALECIPHELLRSRAKERLKQANDAD-AACSLGEEDMLVLDLLQWFKSDFFSWVDNLPCIQCGGKTQPSGSLSPSSDDLHWDAGRVENHFCHTCQLSTRFPRYNN-PEKLLETRKGRCGEWANCFTLLCRALGLEARYIWDSTDHVWTEVYSQSQRRWIHCDPCENACDKPLLYEVGWGKKLSYILAFSKDQVADVTWRYSCKHPEVLSRRTQVQETWLLHMLNKLNAERQQFLGAERKQQLLQRLLVELVEFISPKSPQP----GE-LGGRMSGSLAWRAARGETGASNAKEDTQENVFTPSESEKESKRFHICYNVTKDCYYRVSNGQETIQGWQKGAWRTENMFRKEEHD-WQMVYLARTEGASFGRMSWKFNCAPAGMKMKSASVRVFSQTFHSGTVRWSL 575          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|75076356|sp|Q4R6F3.1|NGLY1_MACFA (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 360.918 bits (925), Expect = 1.962e-113
Identity = 228/595 (38.32%), Postives = 330/595 (55.46%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTAL----QQVLDEPAPPPPATVTRPP-----VPIVPAPLPMG---HL------------LTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRA--PTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVD--SQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFESDA-LTGSKTLSLRVLMW---GELACRDFRLFATPTSESEAIQLRWVVQL 754
            G  + L  +GFE     L FP   S   L+  R  +       LD          ++ P     +P  P+  P G   H              + +T +S     + +  QHV+ +ENP  Q +A   +P+++L+ ++  +L+R+R+  +  + I   D LLLELL WFK +FF WVN+  C  CGG +  S  R+  P   E   GA  VE   C AC+   RFPRY++ PEKLLETR GRCGEWANCF L CR++GF+ R+V D+TDHVW EV+S ++ RWLH D CE+ CDKPLLYE+GWGKKL+YVIA SKDEV DVT+RYS  H  + +RRTKV+E  L E++  L    Q     NR  +L +R ++ELV  + P    +   GE L GR SGS+AW++ARGEMGL     RK T    P+E +  S+  HL YN+V+D Y RVSN N+ ++GW+ G  ++E + R  ++D W    LARKE    A + WK +     L + S+ +  +S T  +G + W+L  D+  +  + D+   S A  +G+  + L   +    G++A +  +LF    ++ E   L  +++ 
Sbjct:   70 GAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVESSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSPDPPSASTVTPDSAILGVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRVRKLDK-GTNISDEDFLLLELLHWFKEEFFHWVNNILCSKCGGQT-RSRDRSLLPNDDELKWGAKKVEDHYCDACQFSNRFPRYNN-PEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEEVIARRTKVKEALLRETINGLNKQRQLFLSENRRKELLQRIIVELVEFISP---KTPKPGE-LGGRISGSVAWRVARGEMGL----QRKET-SFIPSENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVETD-WHMVYLARKEGSSFAYISWKFECGSVGLKIDSISIRTTSQTFQTGTIEWKLRSDTARVELTGDNNLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKF 651          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|74732105|sp|Q96IV0.1|NGLY1_HUMAN (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; Short=hPNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 360.147 bits (923), Expect = 3.590e-113
Identity = 230/596 (38.59%), Postives = 329/596 (55.20%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTAL----QQVLDEPAPPPPATVTRPP-----VPIVPAPLPMGHLLTYMTN----------------ESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRA--PTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVD--SQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFESDA-LTGSKTLSLRVLMW---GELACRDFRLFATPTSESEAIQLRWVVQL 754
            G  + L  +GFE     L FP   S   L+  R  +       LD          ++ P     +P  P+  P G L  +  N                +S   + + +  QHV+ +ENP  Q +A   +P+++L+ ++  +L+R R+  +    I   D LLLELL WFK +FF WVN+  C  CGG +  S  R+  P+  E   GA  VE   C AC+   RFPRY++ PEKLLETR GRCGEWANCF L CR++GF+ R+V D+TDHVW EV+S ++ RWLH D CE+ CDKPLLYE+GWGKKL+YVIA SKDEV DVT+RYS  H  + +RRTKV+E  L +++  L    Q     NR  +L +R ++ELV  + P    +   GE L GR SGS+AW++ARGEMGL     RK T    P E +  S+  HL YN+V+D Y RVSN N+ ++GW+ G  ++E + R  ++D W    LARKE    A + WK +     L V S+ +  SS T  +G V W+L  D+  +  + D++  S A  +G+  + L   +    G++A +  +LF    ++ E   L  +++ 
Sbjct:   70 GAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSSNPSG-LNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLDK-GINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQT-RSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNN-PEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRIIVELVEFISP---KTPKPGE-LGGRISGSVAWRVARGEMGL----QRKETL-FIPCENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVETD-WHMVYLARKEGSSFAYISWKFECGSVGLKVDSISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKF 651          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|82081682|sp|Q5ZJM3.1|NGLY1_CHICK (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 359.377 bits (921), Expect = 6.247e-113
Identity = 218/563 (38.72%), Postives = 309/563 (54.88%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTAL---------------QQVLDEPAPPPPATVTRP--PVPIVPAP---LPMGHLLTYMTNESEFYQKMVAESQH-VMKFENPQAQSRARRVVPIEKLESRA---LAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSV-HSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVD--SQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFESDA-LTGSKTLSLRVLMWG 726
            G  + L  +GF+     + FP   S   LR  R  +               +    E      A   +P  P   VP P    P   LL  +   ++    + ++  H ++ +E+   Q +A  ++P+++L+ +A   LAQ  RL +       +   D LLLELL WFKNDFF WV++  C  CGG +     Y +PT  +    A  VE   C  C+   RFPRY++ PEKLLETRRGRCGEWANCF L CR++GF+ R+V DWTDHVW EV+S ++ RWLH DPCEN CDKPLLYE+GWGKKL+YVIA SKDEV DVT+RYS  H  + +RRT + E  L E++  +    Q+     R  +L ER ++EL   + P    +   GE   GR SGS+AW++ARGE G +E    ++     P+E +  S++FHL YNVV D YTR+SN NE + GW  G  + E + R  ++D W    LARKE    AS+ WK +     L + ++ +  SS T  SGR+ W+LY  +  ++   D++  S +   G+  ++L   + G
Sbjct:   69 GAVECLFEMGFQEGETHMVFPKEASIEQLRKVRDLIAVERSSRLNESNQVHRSASSETVAITQAIAHQPSRPAGSVPTPDHQQPEPSLLQSLKMAADILTTLQSKCDHLILAYESTSLQQKALALIPLQQLKEKAQRKLAQATRLDKGEH----VNEEDFLLLELLNWFKNDFFHWVDNLPCSRCGGQTEGKRDYLSPTDDDLRWNADRVENHYCSQCQFCNRFPRYNN-PEKLLETRRGRCGEWANCFTLCCRAVGFEARYVRDWTDHVWTEVYSASQKRWLHCDPCENVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHQEVLTRRTALSEAKLRETINAINKKKQQSLSEGRRKELLERTIVELAEFISP---KTPKPGE-FGGRTSGSMAWRIARGETGSEE----RKEVIFIPSEKEKASKLFHLMYNVVEDSYTRISNNNEQITGWGTGIWKAESIARKVETD-WKMVYLARKEGSSSASISWKFECKSVGLKIDNISIRTSSQTFHSGRIKWRLYSPTAEVILVGDNSLSSYSDFCGATEVTLEATLSG 617          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|114152093|sp|Q9JI78.2|NGLY1_MOUSE (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; Short=mPNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 344.739 bits (883), Expect = 2.453e-107
Identity = 220/593 (37.10%), Postives = 318/593 (53.63%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQ--------------QVLDEPAPPP-PATVTRPPVPIV------PAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSS-VHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVD--SQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFES-DALTGSKTLSLRVLMW---GELACRDFRLFATPTSESEAIQLRWVVQLRE 756
            G  + L  +GFE     L FP   S   L+  R  +               Q    PA    P   +  P  ++         LP       +  +S   + + +  QHV  +ENP  Q +A   +P+ +L+ +A  +L R R+  +  + +   D LLLELL WFK +FF WVN+  C  CGG +        P   E   GA  VE   C AC+   RFPRY++ PEKLLETR GRCGEWANCF L CR++GF+ R+V D+TDHVW EV+S ++ RWLH D CE+ CDKPLLYE+GWGKKL+Y+IA SKDEV DVT+RYS  H  + SRRTKV+E  L E++  L    Q     +R  +L +R ++ELV  + P    +   GE L GR SGS+AW++ARGE GL+     ++     P+E +  S+ FHL Y++VRD Y RVS+ N  ++GW+ G  ++E + R  + D W+   LARKE    A + WK +     L V +V +  SS +  SG V W+L  ++  +    D    S +  +G+  ++L   +    G++A +  +LF    ++S    L  ++   +
Sbjct:   70 GAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDK-GTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNN-PEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISP---KTPRPGE-LGGRVSGSLAWRVARGETGLE-----RKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKD-WNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFND 650          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|114152094|sp|Q5XI55.2|NGLY1_RAT (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; Short=PNGase; AltName: Full=N-glycanase 1; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 339.347 bits (869), Expect = 2.557e-105
Identity = 206/528 (39.02%), Postives = 293/528 (55.49%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFR------------------------TALQQVLDEPAPPPPATVTRPPVPIVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMD-GASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVD--SQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQL 692
            G  + L  +GFE     L FP   S   L+  R                         A++   ++P  P          P  P  LP   L   +  +S  ++ + +  QHV  +ENP  Q +A   +P+ +L+ ++  +L R R+  +  + +   D LLLELL WFK +FF WVN+  C  CG  +       P + +++  GA  VE   C AC+   RFPRY++ PEKLLETR GRCGEWANCF L CR++GF+ R+V D+TDHVW EV+S ++ RWLH D CE+ CDKPLLYE+GWGKKL+Y+IA SKDEV DVT+RYS  H  + SRRTKV+E  L E++  L    Q     +R  +L +R ++ELV  + P    +   GE L GR SGS+AW++ARGE  L+     ++     P+E +  S+ FHL Y++VRD Y RVS+ N  ++GW+ G  ++E + R  + D W+   LARKE    A + WK +     L V +V +  SS +  +G V W+L
Sbjct:   70 GAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAVERRSRLDGSSQKVEFSQHPAAVRLPAEQPEDPTGLMQHSGNQPGQPLSLPSAPL---VVGDSTIFKVLQSNIQHVQLYENPVLQEKALACIPVNELKRKSQEKLFRARKLDK-GTKVSDEDFLLLELLHWFKEEFFHWVNNVVCSRCGRETRSRDEALPPNDDELKWGAKNVEDHYCDACQLSNRFPRYNN-PEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHEEVMSRRTKVKEELLRETINGLNKQRQLLLSESRRKELLQRIIVELVEFISP---KTPRPGE-LGGRVSGSLAWRVARGETCLE-----RKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNANISGWENGVWKMESIFRKVEKD-WNMVYLARKEGSSFAYISWKFECGSAGLKVDNVSIRTSSQSFETGSVRWKL 582          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|74876519|sp|Q7KRR5.1|NGLY1_DROME (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 250.366 bits (638), Expect = 5.114e-72
Identity = 160/520 (30.77%), Postives = 261/520 (50.19%), Query Frame = 0

Query:  195 GLFQILRALGFER--DGHGLHFPTYGSFYVLRAFRTALQQVLD-------EPAPPPPATVTRPPVPIVPAPLPMGHLLTY---MTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQL----NRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHL-LEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIV------NGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRV 688
            G  ++L A+GF R    +    PT  S   ++ +R AL +            +PP  +T +  P+ I P+ +   H + +   +   + F Q +   S  VM++E+    +  R ++P+E+L   A  +L    +++    R      +RDLLL+EL+ WF   FF WVN+  C  CG         +   + + +G   VE  VC  C QE +F RY+   + LL +R+GRCGE+ANCF  +CR++ +D R V    DHVW EV+S+A+ RWLH DP EN  D PL+Y+ GW + + Y++A S+D++QDVT+RY+ DH  +   R    E+ + ++L  +    ++    +R L L++R + E++ L LE   +P+  +   L GR SGS++W+ +RGE            + L+  E+  +  ++ Y+   D Y R +   E +        W       + + R  + D W    LAR ED     + W  D SKT L V+S  L   + T   G++
Sbjct:   74 GCERLLEAIGFVRAPSSNAYTLPTEVSLQQVKKYRDALSERRTAWLNGTVSKSPPQQSTTSTTPLFIKPS-VEYRHRIAFPRVLRTNNNFLQSLELYSDAVMQYEDNLLLATGRTLIPVEELTEMASEKLIDIQDQIASGERQEKEPCVRDLLLVELVNWFNTQFFQWVNNIPCRVCGSEE------SRLRRTEREGDIRVEVTVC--CGQESKFYRYNDISQ-LLVSRKGRCGEYANCFTFLCRALDYDARIVHSHFDHVWTEVYSEAQMRWLHVDPSENVIDSPLMYQHGWKRHIDYILAYSRDDIQDVTWRYTNDHQKILHLRKLCGEKEMVQTLDAIRAKRRQNCTADRKLFLSQRNMYEVIGLTLER--KPTENE---LKGRSSGSLSWRQSRGEHTFTNIF----VFNLSATELQKRQLNVRYSCATDTYERYAKEGEHITILDSYKTWQKAQFSSKNIFRKVERD-WKMAYLARLEDTDCGEIAWTFDFSKTNLKVKSYNLVFETKTFGDGKI 573          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|221222469|sp|Q28YQ7.2|NGLY1_DROPS (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; AltName: Full=Peptide:N-glycanase)

HSP 1 Score: 244.202 bits (622), Expect = 9.454e-70
Identity = 165/529 (31.19%), Postives = 253/529 (47.83%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFY---------------VLRAFRTA-LQQVLDEPAPPPPATVTRPPVPIVPAPLPMGHLLTY---MTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRA----LAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHL-LEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAP--AEVDSQVFHLAYNVVRDEYTRVSNGN------EIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRV 688
            G  ++L A+GF       H P+  S+                VLR  RTA L   + +    P A  + P    +   +P    +T+   +   + F Q +   S  VM++E+    +  R ++P++ L S+A    +A  + +           +RDLLL+EL  WF   FF WVN+  C  CG         +   + Q +G   VE  VC  C QE +F RY+    +LL +R+GRCGE+ANCF  +CR + +D R V    DHVW EVFS+ + RWLH DP +N  D PL+Y+ GW + + YV A S+D+ QDVT+RY+ +H  +   R    E+ L ++L  +    Q+     R   L++R + E++ L LE   +P+  +   L GR SGS++W+ +RGE            +  +P  AE+     +L Y+   D Y R +  +           W       + + R  + D W    LAR ED   A + WK DL+KT L V+S KL   + T   G++
Sbjct:   73 GCEKLLYAIGFR------HTPSSNSYTLPGEVSLQQIQKYYDVLRERRTAWLSGAMPKSPAHPKAACSTPSPLFIKTSVPYRQRITFPRVLRTSNRFLQSLELYSDAVMQYEDELLLATGRSLIPVDDLTSKASEKMMAMQDLIASGECSEKEPCIRDLLLVELTNWFNTQFFEWVNNIQCQVCGSEE------SKLRRTQTEGDVRVEVNVC--CGQESKFYRYND-ISQLLVSRKGRCGEYANCFTFLCRCLDYDARLVHSHFDHVWTEVFSETQMRWLHVDPSDNVVDSPLMYQHGWKRGIDYVFAYSRDDAQDVTWRYTNNHQEILKLRKLCDEKELIQTLNAIREKRQQNCSAERKNFLSQRNMFEVIGLTLER--KPTENE---LKGRSSGSLSWRQSRGEHTF------TNIFVFSPNEAELKKCQLNLRYSCATDTYERYTKDDGQITLLNTYKTWQSAQFSSKNIYRKVERD-WKMAYLARLEDTDCAEIIWKFDLTKTNLKVKSYKLVFETKTFGEGKI 574          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|75024657|sp|Q9TW67.1|NGLY1_CAEEL (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; AltName: Full=Peptide:N-glycanase; Short=PNGase)

HSP 1 Score: 243.047 bits (619), Expect = 1.288e-69
Identity = 151/423 (35.70%), Postives = 225/423 (53.19%), Query Frame = 0

Query:  268 NESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRA-PTSQEQMDG-ASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSH-----TDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDSQVF-HLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSIS 679
            +E  F ++ V  S     +++   ++ AR V+P E+L  RA+ +  R  +A            +L +LL WFK  FF W + P C  C       G +  PT +EQ +G AS VE ++C  C  E+RFPRY++ P KLL+TR GRCGEWANCF L+  ++  ++R + D TDHVW EV+  AE RW H DPCENT D+PLLY  GWGK L Y I    D V DVT+RY  D   L ++R +VR+      L KL +    G    R  +L  RR+ EL+ ++   +  +H       G+ L GR +GS  W+  RGE+G           +LAP    +Q +    Y+V+ D Y   S   EI  G+     E+E ++R++++D W+   L RK      ++ W  DL   + S++ +++ ++
Sbjct:  120 SEKRFLEQFVKCSNVPRSYQDEVFKALARSVMP-EELVGRAMTEGPRDEKA------------ILKDLLHWFKTQFFTWFDRPTCPKCTLKCSTDGLQGTPTREEQKEGGASRVEVYICDGCNTEMRFPRYNN-PAKLLQTRTGRCGEWANCFGLLLAALNLESRFIYDTTDHVWNEVYLLAEQRWCHVDPCENTMDRPLLYTRGWGKTLGYCIGYGSDHVVDVTWRYIWDSKKLVTQRNEVRQPVFENFLSKLNSRQAEGQTEPRKRELAVRRVCELMEMMAQ-EAKNHKIGWEKIGDDLGGRITGSEEWRRERGELGESGPKLLAEPIKLAPPTGPAQNYLEFNYDVITDTY---SQPPEI--GFSAQAFELENVQRVEETD-WNMTYLCRKRGDAPGNISWHFDLKSLKKSIEKIEIRMA 521          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. SwissProt
Match: gi|75170522|sp|Q9FGY9.1|PNG1_ARATH (RecName: Full=Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; AltName: Full=Peptide:N-glycanase; Short=AtPNG1)

HSP 1 Score: 240.35 bits (612), Expect = 1.114e-67
Identity = 138/319 (43.26%), Postives = 182/319 (57.05%), Query Frame = 0

Query:  268 NESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRG--FERNRMLQLTERR-LIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDED 583
            +  EF  ++      V+ +E+P  Q  AR+ VP ++LE +AL  L   +  +   S        LL+LL WFK  F  WVN P C FCG  ++  G   P + E   GA+ VE + C  C    RFPRY+  P KL+ET++GRCGEWANCF L CR+ G+D+R ++D+TDHVW E +S +  RW+H DPCE   DKP+LYE GW KKL YVIA SKD V DVT RY++    + SRRT   E  L + L  LT + +R   FE    L+L +R    EL   L   D  S +    L GRQSG   W++ R E G DE+
Sbjct:  112 DNGEFEGRIRPYVSQVLMYEDPVRQDAARKTVPKDELEEKALVSL--AKEGNFEPSKEERDYAFLLQLLFWFKKSF-RWVNEPPCDFCGNKTIGQGMGNPLTSELAYGANRVEIYRCTMCPTTTRFPRYND-PLKLVETKKGRCGEWANCFTLYCRTFGYDSRLIMDFTDHVWTECYSHSLKRWIHLDPCEGVYDKPMLYEKGWNKKLNYVIAISKDGVCDVTKRYTKKWHEVLSRRTLTTESSLQDGLRTLTRERRRSLMFESLSKLELRDRNEQEELERNLHSADNASVS----LPGRQSGDREWRIMRSEFGSDEN 422          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|1325305867|ref|XP_023334551.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Eurytemora affinis])

HSP 1 Score: 473.781 bits (1218), Expect = 2.175e-152
Identity = 303/825 (36.73%), Postives = 437/825 (52.97%), Query Frame = 0

Query:    5 DLLSQTVRT-AAGSRVQVREALGERAVLALYFVHPLAEADRRFTAELGPAYRTYGSAGP-VGLLLVPLADSPLDPALEALA----------LPVWLAGPSLAARYGVGYTPCLVVVDAQDGSVITRDGGTAVTRQGHAAFASWEPPAQPDVDTSSVAALGANPDPWQRVVAQMLVDRVAQLLS--GSAAARSFGLCDPRWAE----ASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLD--------------------------EPAPPPPATVTRPPVPIVPAPLPMGHLLTYMTN---------------------ESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKE-DVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFESDALTGSKTLSLRVLMW---GELACRDFRLF-ATPTSESEAI-QLRWVVQLR 755
            DLLS  V     G R  +++ L ++  +ALYF        R FT  L  AYR     GP  G+ ++ ++    +    +            +P   A   ++A++ +   P L V+   DGS+I+ DG   V   G  +F  WE  A   +D S V  L  N    +     +L+   + ++   G+   R   L +P+       A+G F++L + GFE D   L  P       +  F  A+ ++ D                            +  P    +  P     +  P G L + M +                     E +F++K+V+  QH++ +E+P AQ+ A  V+P+E+ ES A  +L R +      S ++  D  L+E   WFK +FF WV++P C  C G + + G   PT +EQ DGA  VEG+VC++C  E RFPRYHSKP KLLETRRGRCGEWANCF L CR++GFDTRH+LDWTDHVW EV+S AE+RWLH DP E   DKPL+YE GWGKKLTYVIA+SKDEVQDVT+RYS+DHAA + RR  VR +WL  SLL+LT   Q  +   R  +L +RRL + + +L P       +GE++ GRQ+GS+AW++ARGE+G  E      T+  +  +V+   F L   + ++ Y  + +G  I  GW  G  E E + RI +SD W+   L+R+E ++G+ S+ WK+ L     S   V+L ++S+   +GRV+WQL G+S  LLP P    ++D LTGS  L L  LM    G+LA +  +LF  + T E +A   LR VV+L+
Sbjct:    2 DLLSSAVIVDKNGRRCDIKDILKDKVGVALYFSAHWCPPCRAFTPRLAQAYRDSRIQGPGKGIEVIFVSSDRSETEFYSYMKEAHGDWFGLVPGQAASQLISAQFSIRGIPALKVI-GLDGSLISEDGRQEVMSLGSRSFQQWESLAPAPIDVSVVNLLLDNEPTVKNEACDILIRLFSNIIKDPGNIKYRQVKLTNPKIESKLLPAAGAFEVLFSAGFEEDDDKLLLPLAIPTSKIEKFHDAIMKIKDASQGSLSSSGSKPGASGGSGSETSGSGGSGAPTQGSSSLPGGSASSSGPAGGLSSLMESGGSSSNIQGSSRLLMPDVLAREKDFHRKLVSGYQHMLTYEDPIAQAAALSVMPVERFESAAKEKLERAKTLDPRLSDLLYSDFKLIEFKEWFKTEFFKWVDAPKCSTCNGDTENKGMLVPTIEEQADGAGRVEGYVCKSCGAETRFPRYHSKPAKLLETRRGRCGEWANCFVLCCRALGFDTRHILDWTDHVWSEVWSPAEDRWLHVDPGE-AVDKPLVYEAGWGKKLTYVIASSKDEVQDVTWRYSKDHAATKQRRNIVRPKWLIRSLLQLTEKAQETYTSERKAKLAQRRLADCLEMLMPR---KAGEGEKV-GRQTGSLAWRLARGELG--EPSSTSFTFTPSAQDVEKTEFCLELFMNKNYYL-LGDGTRI-EGWRSGILEAENVTRIVESD-WNMVYLSRQEGNLGKGSVTWKVKLPPGYTSGH-VELLVNSTVFKTGRVIWQLCGESSCLLPQPGVNLQTDQLTGSNELKLTALMSGGEGDLAWQHTQLFRCSSTGEGDANPNLRLVVKLK 814          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|919002046|ref|XP_013410246.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Lingula anatina])

HSP 1 Score: 410.994 bits (1055), Expect = 1.612e-130
Identity = 230/582 (39.52%), Postives = 342/582 (58.76%), Query Frame = 0

Query:  193 ASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLDEPAPPPPA--TVTRPPVPIVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASG----IMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRT-----WELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFE-SDALTGSKTLSLRVLM---WGELACRDFRLFATPTSESEAIQLRWVVQLRE 756
             SG  + L A+GF+ DG  L  P       +R+ R  L +V  +      A     + P P           +  M  E  FY K+ +   HV+ +E+P  Q++AR  +P++++ + A  + + L  AH  A      I + D+L+LEL+ W+K DFF WVN+PNC  CGG++ ++G   PT++++  GA  VE + C  C + VRFPRY+  P KLLETRRGRCGE+ANCF L CR++GF+ R+VLDWTDHVW EV+S+++ RWLH D CE  CDKPLLYEVGWGKKLTYVIA SKD++QDVT+RY+R+   + SRR + RE WL  ++L +  + Q+     R+ +L  R L+ELV  + P  +    +GE + GRQSGS+AW++ARGE+G    P + +T     ++L   E + ++FHL Y    D Y RVSN +E V GW  G  E + + R ++ D W    LAR+E    AS+ WK+D + + L V +V++  SS     G++ W+L GD++  +P  +S    +D L G + L+L   +    G++A +  +LF     E        V++L++
Sbjct:   61 VSGGMECLFAMGFQEDGEYLRLPRNVLLDEIRSVRDELHEVRQQQVNQQGAGQPKPQQPTPQTTPLQTPAFDVALMAKEMTFYSKLKSSFDHVLVYEDPALQAKARDCMPLQEMRAEAQKKFDELS-AHSKAMDKPVEIDVNDILILELVNWYK-DFFSWVNAPNCDSCGGATQNAGMAEPTAEDRKWGAGRVENYWCGTCRKHVRFPRYNH-PGKLLETRRGRCGEYANCFTLCCRAVGFEARYVLDWTDHVWTEVYSRSQKRWLHCDTCEKGCDKPLLYEVGWGKKLTYVIAFSKDDIQDVTWRYTRNFQEILSRRNECRENWLVNTILAMRKERQKHLPPARIQELEHRTLVELVEFMTP--KKELKEGEDV-GRQSGSLAWRIARGEVGA---PPKLKTNTPVIFKLTDVEKEKKLFHLKYCCASDTYIRVSNDSEEVKGWQAGVFEADSVFRKEEHD-WKMVYLARREGAATASITWKVDCTDSGLLVDTVEVLASSCVYEDGKISWRLCGDNQICMPIKESKRTLTDDLNGCRELTLTAELSKGRGDVAWQHTQLFRQQKDEVHNFPFEIVIKLKK 632          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|762133729|ref|XP_011450075.1| (PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase isoform X1 [Crassostrea gigas] >gi|405952637|gb|EKC20424.1| Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Crassostrea gigas])

HSP 1 Score: 408.683 bits (1049), Expect = 2.653e-129
Identity = 225/587 (38.33%), Postives = 325/587 (55.37%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLD----------EPAPPPPATVTRPP---------------------VP----IVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPA-SGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKR--TWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFES-DALTGSKTLSLRVLM---WGELACRDFRLF 736
            G  + L  +GF  DG  L  P   S  ++R  R  L + ++             P PP T+  PP                     VP    + P PL           E  F+ K+     HV+ +E+P  Q +AR ++P+E L+  A  +L  +++A + A + I  RDL+LLELLAWFKN FF WV++P C  CGG +   G   PT  E    A+ VE + C  C++ VRFPRY+  PEKLLETR GRCGEWANCF L CR++GF+ R+VLDWTDHVW EV+S+ + RWLH DPCEN CDKPLLYE GWGKKLTYV+A SKDEVQDV++RYS   A +  RR + RE WL + + +L    +     +R  ++  R LIELV  +     P  +DG+ LSGR +GS+AW+MARGE+G  + P       ++L   E  S+VFH+ Y+  +DEY RVS  +E++  +     + + + R ++ D W    LAR E    A + WK D S   + +  +++  SS T  +G + W+L   ++  + + D   ++   L+G   ++L  ++    GE+A +  ++F
Sbjct:   62 GALECLFEMGFVEDGEFLTLPPNTSLEIIRQIRKDLNEYVETMGKVNGEQASQTPTPPVTIRAPPQAPATTGAPSQTPSTTRAPSQVPATTRMPPNPLQDKSQQEINQMERRFFSKIQNSLVHVLMYEDPTLQKKARAIIPVETLKKEAHRKLEEIKKAEKAAVTSIDFRDLMLLELLAWFKNSFFSWVDAPKCDNCGGETQSVGMAEPTPDEIRWQANRVENYKCNRCQRFVRFPRYNH-PEKLLETRCGRCGEWANCFTLCCRAVGFEARYVLDWTDHVWTEVYSEIQKRWLHCDPCENVCDKPLLYEAGWGKKLTYVLAFSKDEVQDVSWRYSAKQAEMLGRRNECRESWLVQVVHRLWKAKEPSNSPDRNEEMKRRLLIELVEFM----TPKSSDGQNLSGRTTGSLAWRMARGEIGSSQAPTSAEPFVFKLTEEERRSKVFHVKYSCAKDEYIRVSKNSEVIKPFSSCVNKQQNVFRKEERD-WKMAYLARTEGSSSAEISWKFDFSG--MEIDKIEICTSSQTFENGVISWRLCSKNQCAMLTGDPEVKTYSELSGEDEMTLTAMLSGGKGEVAWQHTQMF 640          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|919068819|ref|XP_013415884.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Lingula anatina])

HSP 1 Score: 407.527 bits (1046), Expect = 4.735e-129
Identity = 229/582 (39.35%), Postives = 341/582 (58.59%), Query Frame = 0

Query:  193 ASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLDEPAPPPPA--TVTRPPVPIVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASG----IMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRT-----WELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFE-SDALTGSKTLSLRVLM---WGELACRDFRLFATPTSESEAIQLRWVVQLRE 756
             SG  + L A+GF+ DG  L  P       +R+ R  L +V  +      A     + P P           +  M  E  FY K+ +   HV+ +E+P  Q++AR  +P++++ + A  + + L  AH  A      I + D+L+LEL+ W+K DFF WVN+PNC  CGG++ ++G   PT++++  GA  VE + C  C + VRFPRY+  P KLLETRRGRCGE+ANCF L CR++G++ R+VLDWTDHVW EV+S+++ RWLH D CE  CDKPLLYEVGWGKKLTYVIA SKD++QDVT+RY+R+   + SRR + RE WL  ++L +  + Q+     R  +L  R L+ELV  + P  +    +GE + GRQSGS+AW++ARGE+G    P + +T     ++L   E + ++FHL Y    D Y RVSN +E V GW  G  E + + R ++ D W    LAR+E    AS+ WK+D + + L V +V++  SS     G++ W+L GD++  +P  +S    +D L G + L+L   +    G++A +  +LF     E        V++L++
Sbjct:   61 VSGGMECLFAMGFQEDGEYLRLPRNVLLDEMRSVRDELHEVRQQQVNQQGAGQPKPQQPTPQTTPLQTPAFNVALMAKEMTFYSKLRSSFDHVLVYEDPALQAKARDCMPLQEMRAEAQKKFDELS-AHSKAMNKPVEIDVNDILILELVNWYK-DFFSWVNAPNCDSCGGATENAGMAEPTAEDRKWGAGRVENYWCGNCRKHVRFPRYNH-PGKLLETRRGRCGEYANCFTLCCRAVGYEARYVLDWTDHVWTEVYSRSQKRWLHCDTCEKGCDKPLLYEVGWGKKLTYVIAFSKDDIQDVTWRYTRNFQEILSRRNECRENWLVNTILAMRKERQKHLPPARAQELEHRTLVELVEFMTP--KKELKEGEDV-GRQSGSLAWRIARGEVGA---PPKLKTNTPVIFKLTDVEKEKKLFHLKYCCASDTYIRVSNDSEEVKGWQAGVFEEDSVFRKEEHD-WKMVYLARREGAATASITWKVDCTDSGLLVDTVEVLASSCVYEDGKISWRLCGDNQICMPIKESKRTLTDDLNGCRELTLTAELSKGRGDVAWQHTQLFRQQKDEVHNFPFEIVIKLKK 632          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|762133731|ref|XP_011450076.1| (PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase isoform X2 [Crassostrea gigas])

HSP 1 Score: 395.201 bits (1014), Expect = 3.513e-125
Identity = 211/512 (41.21%), Postives = 288/512 (56.25%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLD----------EPAPPPPATVTRPP---------------------VP----IVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPA-SGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKR--TWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLS 665
            G  + L  +GF  DG  L  P   S  ++R  R  L + ++             P PP T+  PP                     VP    + P PL           E  F+ K+     HV+ +E+P  Q +AR ++P+E L+  A  +L  +++A + A + I  RDL+LLELLAWFKN FF WV++P C  CGG +   G   PT  E    A+ VE + C  C++ VRFPRY+  PEKLLETR GRCGEWANCF L CR++GF+ R+VLDWTDHVW EV+S+ + RWLH DPCEN CDKPLLYE GWGKKLTYV+A SKDEVQDV++RYS   A +  RR + RE WL + + +L    +     +R  ++  R LIELV  +     P  +DG+ LSGR +GS+AW+MARGE+G  + P       ++L   E  S+VFH+ Y+  +DEY RVS  +E++  +     + + + R ++ D W    LAR E    A + WK D S
Sbjct:   62 GALECLFEMGFVEDGEFLTLPPNTSLEIIRQIRKDLNEYVETMGKVNGEQASQTPTPPVTIRAPPQAPATTGAPSQTPSTTRAPSQVPATTRMPPNPLQDKSQQEINQMERRFFSKIQNSLVHVLMYEDPTLQKKARAIIPVETLKKEAHRKLEEIKKAEKAAVTSIDFRDLMLLELLAWFKNSFFSWVDAPKCDNCGGETQSVGMAEPTPDEIRWQANRVENYKCNRCQRFVRFPRYNH-PEKLLETRCGRCGEWANCFTLCCRAVGFEARYVLDWTDHVWTEVYSEIQKRWLHCDPCENVCDKPLLYEAGWGKKLTYVLAFSKDEVQDVSWRYSAKQAEMLGRRNECRESWLVQVVHRLWKAKEPSNSPDRNEEMKRRLLIELVEFM----TPKSSDGQNLSGRTTGSLAWRMARGEIGSSQAPTSAEPFVFKLTEEERRSKVFHVKYSCAKDEYIRVSKNSEVIKPFSSCVNKQQNVFRKEERD-WKMAYLARTEGSSSAEISWKFDFS 567          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|171460972|ref|NP_001116357.1| (N-glycanase 1 S homeolog [Xenopus laevis] >gi|115527879|gb|AAI24907.1| LOC733299 protein [Xenopus laevis] >gi|1050371971|gb|OCT74171.1| hypothetical protein XELAEV_18033126mg [Xenopus laevis])

HSP 1 Score: 397.127 bits (1019), Expect = 4.306e-125
Identity = 223/532 (41.92%), Postives = 312/532 (58.65%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTAL----QQVLDEPAPPPPATVTRPPV--------PIVPA-PLPMGH-LLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFES 709
            G  + L  +GFE +   L FP   S   LR  R  +     + +    P PPAT + PP         P+ P+ PLP    +  ++ +E  + + + + SQHV+ +E P+ Q RA + +P+  L++RA  +L   +      S + L D LLLELL WFK DFF WVNS  C  CGG +      +P+ ++   GA+ VE   C+ C+   RFPRY+  PEKLLETRRGRCGEWANCF L CR++GF+ R+V D TDHVW EV+S ++NRWLH DPCEN CDKPLLYEVGWGKKL+Y+IA SKDEV DVT+RYS  H  + +RR +VRE WL E+++ L    Q+    +R  +L  R ++ELV  +     P  T    L GR SGS+AW++ARGE  L     +   +  + +E  S+ FHL YNVV D YTR SN N ++ GW+ G  + E + R  +SD W    LAR E    A++ WK++ +   L ++S+ +  SS T  SG+++W+L      +  + D+ F S
Sbjct:   62 GAIECLFEMGFEEEETHLVFPKMASVEKLRNVRDHIAIERNKRMSAANPTPPATSSTPPAFSRTPVPNPVPPSIPLPATEDISNFLASEVRYLKTLQSNSQHVLIYEEPEIQQRALQEIPVSDLKTRAQKKLIEAKSLDSDTS-VNLEDFLLLELLRWFKQDFFQWVNSLPCSHCGGETQARDALSPSDEDLRWGANRVENHYCEKCKHSNRFPRYN-HPEKLLETRRGRCGEWANCFTLCCRALGFEARYVWDSTDHVWTEVYSSSQNRWLHCDPCENACDKPLLYEVGWGKKLSYIIAFSKDEVVDVTWRYSCKHEDVIARRKEVRESWLRETIVGLNKMRQQSVPEHRKQELLGRLIVELVEFM----SPKTTKPGELGGRVSGSVAWRVARGETSLQS--TKSVIFIPSESEKTSKRFHLQYNVVEDTYTRTSNNNVVIAGWENGAWKAESICRKVESD-WKMVYLARTEGSSSAAISWKIECASAGLQIESISVRTSSQTFHSGKIIWKLSSPEAEVEVNSDTNFHS 584          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|301605650|ref|XP_002932449.1| (PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Xenopus tropicalis] >gi|1043419104|gb|OCA31735.1| hypothetical protein XENTR_v90016440mg [Xenopus tropicalis])

HSP 1 Score: 396.741 bits (1018), Expect = 5.219e-125
Identity = 217/534 (40.64%), Postives = 307/534 (57.49%), Query Frame = 0

Query:  195 GLFQILRALGFERDGHGLHFPTYGSFYVLRAFR----TALQQVLDEPAPPPPATVTRPPVP----------IVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPAEVDS--QVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSRGLLPSPDSTFES 709
            G  + L  +GFE     L FP   S   LR  R    T   + +      P AT + PP+                     + +++ +E  + + + + SQHV+ +E  + Q RA + +P+  L++RA  +L  ++      +G+ + D LLLELL WFK DFF WVNS  C  CGG +      +P++++   GA+ VE   C+ C+   RFPRY+  PEKLLETRRGRCGEWANCF L CR++GF+ R+V D TDHVW EV+S ++NRWLH DPCEN CDKPLLYEVGWGKKL+Y+I  SKDEV DVT+RYS  H  + +RR +VRE WL E++  L    Q     NR  +L  R ++ELV  + P    +   GE L GR SGS+AW+MARGE  L  +    ++    P+E +   ++FHL YNVV D YTRVSN NE++ GW+ G  + E M R  ++D W    LAR E    A++ WK++ +   L ++S+ +  S  T  SG++ W+L      +  + D++  S
Sbjct:   62 GAIECLFEMGFEEGETHLVFPKMASVEKLRKVRDHIATERNKRMSGANSTPQATPSTPPMSSRSPVPNPVPPSIPLQATEDISSFLASEVRYLKTLQSNSQHVLIYEQSELQQRALQEIPVLDLKTRAQQKLTEVKSID-SDTGVNIEDFLLLELLRWFKQDFFQWVNSLPCCLCGGETQGRDALSPSAEDLRWGANRVENHYCEKCKHSNRFPRYN-HPEKLLETRRGRCGEWANCFTLCCRALGFEARYVWDSTDHVWTEVYSTSQNRWLHCDPCENACDKPLLYEVGWGKKLSYIIGFSKDEVVDVTWRYSCKHEDVIARRKEVRESWLRETITGLNKMRQVSLPENRKQELLGRLIVELVEFMSP---KTPKPGE-LGGRVSGSLAWRMARGETSLQSN----KSVVFIPSEREKIGKLFHLRYNVVEDAYTRVSNNNEVITGWENGVWKAESMCRKVEND-WKMVYLARTEGSSSAAISWKIECASVGLQIESLSVRTSGQTFHSGKIKWKLTSPEVEVEVNSDTSLHS 584          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|1207919146|ref|XP_021348446.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Mizuhopecten yessoensis] >gi|1205905787|gb|OWF52816.1| Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Mizuhopecten yessoensis])

HSP 1 Score: 395.971 bits (1016), Expect = 1.179e-124
Identity = 218/559 (39.00%), Postives = 316/559 (56.53%), Query Frame = 0

Query:  193 ASGLFQILRALGFERDGHGLHFP-----TYGSFYVLRAFRTALQQVLDEPAPPPPATVTRPPVPIVPAPLPMG--------HLLTYMTN------ESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRK-RTWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSD-EWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSR-GLLPSPDSTFESDALTGSKTLSLRVLMWG 726
             SG  + L  +GF  DG  L FP     T     V     TA + V    +   P+T    P    PA  P            +T M+       E +F+ K+ +   HV+ +E    Q +AR V+P+E+L   A  + + L+ A + AS + L+D +LL LLAWFKN FF WV++P C  C G +   G   P+ ++   GAS VE + C  C + VRFPRY+  P KLL+TRRGRCGEWANCF L CR+MGF+ R+VLDWTDHVW EV+S ++ RWLH DPCEN CDKPLLYEVGWGKKLTYVIA SKDEVQDV++RYS +   + SRR +VRE WL +   +L N+  +     R  +++ R ++ELV  +        +D   LSGR +GS+ W++ARGE G      ++   + L  +E   ++ H+ YN  +DEYTRV++ N+ ++G  +G+C  +     ++ + +W    LAR E    A++ WK D   + L V+++++ + SST  +G + W+L  D++  +L   D       L G   ++L   + G
Sbjct:   60 VSGALECLFEMGFIEDGEFLTFPASIPPTLTLIQVRNELHTATKLV--RSSTGGPSTGQSTPRVQQPAVTPSAARQIQLPAQTVTPMSRQAMQQAERQFFMKIESSLVHVLNYERRDFQQKARSVIPMEELNKEAKKRFSALQTADKDASAVGLQDCVLLSLLAWFKNSFFKWVDAPECDICQGKTRSVGPAEPSQEDLRWGASRVENYRCDGCNKFVRFPRYND-PGKLLDTRRGRCGEWANCFTLCCRTMGFEARYVLDWTDHVWTEVYSTSQKRWLHCDPCENACDKPLLYEVGWGKKLTYVIAFSKDEVQDVSWRYSANFKEMMSRRKEVRENWLGQVCFRLWNEKNKVVSEERRKEMSNRCIVELVEFI----TQKKSDKNDLSGRTTGSLTWRLARGETGNTPQAIKEPFVFTLTDSEKQGKLLHVRYNCAKDEYTRVTDENKKMSG--FGSCLFKSTNVFRKEEHDWKMVYLARTEGSAIATLSWKFDFKGSGLKVRNIEICVKSSTYENGVLSWRLCSDNQCAILKGTDDLKSVSDLNGVADMTLTANLSG 609          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|1242786352|ref|XP_022333208.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Crassostrea virginica] >gi|1242786354|ref|XP_022333209.1| peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Crassostrea virginica])

HSP 1 Score: 395.586 bits (1015), Expect = 2.938e-124
Identity = 221/578 (38.24%), Postives = 316/578 (54.67%), Query Frame = 0

Query:  193 ASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLDE-------------PAPPPPATVTRP----------------------PVPIVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPAS-GIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKL--TNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKR--TWELAPAEVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLYGDSR-GLLPSPDSTFESDALTGSKTLSLRVLMWG 726
             SG  + L  +GF  DG  L  P   S  +++  R  L + +                +P  PAT T                            +P PL    L      E  F+ K+ +   HV+ +E+P+ Q +A+ ++P+E L+  A  +   +++A   AS  +  RDL+LLELL+WFKN FF WV++P C  CGG +   G   PTS E    A+ VE + C  C++ VRFPRY+  PEKLLETR GRCGEWANCF L CR++GF+ R+VLDWTDHVW EV+SQ + RWLH DPCEN CDKPLLYE GWGKKLTYV+A SKDEVQDV++RYS  H  + SRR + RE WL + + +L    + +   ERN   ++ +R L ELV  +     P  ++G+ L+GR +GS+AW++ARGE+G  + P       + L   E  S++FHL Y+  RDEY RVS   E+   +     + + + R ++ D W    LAR E    A++ WK D S   + ++ V++  +S T  +G + W+L   ++  LL           L+G   ++L  +M G
Sbjct:   58 VSGALECLFEMGFIEDGEFLTLPQTTSLAIIKQIRMDLNKYIQSIERDNGSQESEKVASPQAPATTTTNLNPTAATSAPAATLTPTAATPISTAAIPNPLENKSLQEINQMEKRFFVKIESSLVHVLMYEDPRLQQKAKSIIPVENLKKEAHRKFEEIKKADSAASKNVDFRDLMLLELLSWFKNSFFKWVDAPKCDNCGGDTQSVGMAEPTSDEIRWQANRVENYKCHHCQRFVRFPRYNH-PEKLLETRCGRCGEWANCFTLCCRAVGFEARYVLDWTDHVWTEVYSQIQQRWLHCDPCENACDKPLLYEAGWGKKLTYVLAFSKDEVQDVSWRYSAKHVEMLSRRNECRESWLVQVIHRLWKAREPRNSPERND--EMKKRLLTELVEFM----TPKSSEGQNLTGRTTGSLAWRLARGEIG-SQTPSNSEPFVFNLTDQERKSKLFHLRYSCARDEYIRVSKDGEVTKSFASCLNKHQNIFRKEERD-WRMVYLARTEGSATAAISWKFDFSG--MEIEKVEICAASQTFENGVISWRLCSKNQCALLTGGLEIKTYTELSGEDQMTLTAMMSG 624          

BLAST of peptide-n -(n-acetyl-beta-glucosaminyl)asparagine amidase vs. nr
Match: gi|1188103151|ref|XP_020773341.1| (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Boleophthalmus pectinirostris])

HSP 1 Score: 393.275 bits (1009), Expect = 1.254e-123
Identity = 237/640 (37.03%), Postives = 337/640 (52.66%), Query Frame = 0

Query:  147 SVAALGANPDPWQRVVAQMLVDRVAQLLSG--SAAARSFGLCDPRWAE----ASGLFQILRALGFERDGHGLHFPTYGSFYVLRAFRTALQQVLDE-----------------PAPPPPATVTRPPVPIVPAPLPMGHLLTYMTNESEFYQKMVAESQHVMKFENPQAQSRARRVVPIEKLESRALAQLNRLRRAHRPASGIMLRDLLLLELLAWFKNDFFVWVNSPNCGFCGGSSVHSGYRAPTSQEQMDGASVVEGFVCQACEQEVRFPRYHSKPEKLLETRRGRCGEWANCFALICRSMGFDTRHVLDWTDHVWVEVFSQAENRWLHADPCENTCDKPLLYEVGWGKKLTYVIATSKDEVQDVTYRYSRDHAALRSRRTKVRERWLTESLLKLTNDIQRGFERNRMLQLTERRLIELVHLLEPLDQPSHTDGERLSGRQSGSIAWKMARGEMGLDEDPDRKRTWELAPA--EVDSQVFHLAYNVVRDEYTRVSNGNEIVNGWDYGTCEVEGMERIQQSDEWS---LARKEDVGQASMKWKLDLSKTRLSVQSVKLSISSSTDASGRVVWQLY-GDSRGLLPSPDSTFESDALTGSKTLSLRVLMW---GELACRDFRLFATPTSESEAIQLRWVVQL 754
            +V  L  NP+     VA++L+     +L        RS  + +P ++       G  + L  +GFE     L FP   S   LR  R ++    D+                  APP     ++P  P+   P     +   +T +S F        QHVM +ENPQ Q +A+  +P ++L S A  ++   + A  P   +   D ++LELL WFK DFF WV+   C  CGG + +SG  +P++++   GA  VE   CQ C+   RFPRY++ PEKLLETRRGRCGEWANCF L CR+MG + R++ D TDHVW EV+S ++ RWLH D CEN CDKPLLYEVGWGKKL+Y++A SKD+V DVT+RYS  H  + SRRTKV+E WL  ++  L N  Q+     R  +LTER L+ELV  + P  +P    GE L GR SGS+AW++ARGE     D D+   +   P   E   ++ H+ YN   D+Y RVSN  E+  GW+    + E + R ++ D W    LAR E      + W+ D S   + +QSV +   S T  SG V W++  G +         T    +++GS   S+   +    G++A +  +LF     E++      +VQL
Sbjct:    6 AVTTLCENPNEVFMDVAKLLLTYADNILRNPNEEKYRSIRIGNPTFSTKLLPVKGAVECLFEMGFEEAETHLVFPKTASVEQLRVIRESIAAERDQRLRGSQPTAQAQGGACASAPPSSPGTSQPATPVQQPPTLESSMSFLVTLQSNF--------QHVMVYENPQLQQKAKSCIPNQQLTSAAEKKMKEAKEAD-PDCKLSQEDFVVLELLRWFKQDFFSWVDCLPCSRCGGQTRNSGSLSPSTEDLRWGAQRVENHFCQTCQLSNRFPRYNN-PEKLLETRRGRCGEWANCFTLCCRAMGLEARYIWDSTDHVWTEVYSPSQRRWLHCDSCENVCDKPLLYEVGWGKKLSYILAFSKDQVVDVTWRYSCKHPEVLSRRTKVQEAWLLHTISSLNNTKQQSLSPQRKKELTERLLVELVEFISP-KKPK--PGE-LGGRNSGSLAWRVARGETKAP-DSDKTLGFVFTPTDKEKSDRLLHIRYNASTDQYCRVSNHCEVTPGWEQCVWQREAVFRKEEKD-WQMVYLARTEGSSTGKITWRFDFSPVGMKIQSVTVMAKSQTFQSGTVCWKVQSGQATTEFSGDGQTHPLSSVSGSAEFSIVAELCGGDGDVAWQHAQLFRQSLKETDETSFEILVQL 629