BLAST of prolyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000003803 (supercontig:LSalAtl2s:LSalAtl2s206:247338:248819:-1 gene:EMLSAG00000003803 transcript:EMLSAT00000003803 description:"maker-LSalAtl2s206-augustus-gene-2.13")

HSP 1 Score: 147.132 bits (370), Expect = 2.017e-41
Identity = 85/303 (28.05%), Postives = 144/303 (47.52%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADS-GEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAA 302
            +   G     +P + P+  W+ S R ++ GPEL+  ++R  ++F L PTHEE ++    +   SY+ +PL +YQI  K+RDE+ P+FGL+R  EF+MKD Y+FD+D+   ++ Y  M   Y   F +L + +        S+G ++SHE+H L D+ G+D I    +     N+E                         +V +++ C                           E ++G + P +         K IEV H F LG KYS   ++   +++ +  H  M C+G+G+SR+++A
Sbjct:   75 LQSVGCQRLRMPSLTPSSYWERSHRKSEMGPELMSLKNRRGKEFVLAPTHEEALSQFFSSLRLSYKSMPLYVYQITNKYRDELNPKFGLLRANEFLMKDLYTFDVDQACAKETYKNMSVVYERFFSQLNVPFLRAVGSGASMGSSASHEYHFLTDAVGQDKIGTCNSCEQKFNME-------------------------VVENNYSCP--------------------------ECLNGTIEPLSQ--------KAIEVAHTFHLGNKYSSVFNSDFMNKNAKMEHAEMCCFGIGLSRLLSA 318          

BLAST of prolyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000010574 (supercontig:LSalAtl2s:LSalAtl2s702:126834:130647:1 gene:EMLSAG00000010574 transcript:EMLSAT00000010574 description:"maker-LSalAtl2s702-augustus-gene-1.17")

HSP 1 Score: 53.9138 bits (128), Expect = 4.587e-8
Identity = 34/105 (32.38%), Postives = 50/105 (47.62%), Query Frame = 0

Query:    5 GALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRF-GLMRGREFIMKDAYSFDIDEQ 108
            G  E + P +  A LWK SG W+ Y   + RF+   K  F L P +      I  + +RS+R LP+ +       R+E+     GL+R R F   DA+ F +  Q
Sbjct:  434 GFKEVISPNIYNANLWKTSGHWDHYAENMFRFEIE-KEKFGLKPMNCPGHCLIFDHGVRSWRDLPIRMSDFGVLHRNELSGALTGLLRVRRFQQDDAHIFCMPNQ 537          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|166980149|sp|A8GN90.1|SYP_RICAH (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 320.087 bits (819), Expect = 1.931e-105
Identity = 170/381 (44.62%), Postives = 224/381 (58.79%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+KAGALE L+P +QPA LW ESGR+N+YG E+L+FQDRH      GPT+E++IT+I +++I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDI+E+     Y++MY+AY N F  LG+    V ADNG IGG  SHEFH++A++GE  I Y+K              L      D  +I  +                     Y  A  + D      ++                + I+  KGIEVGHIF +G KYS ++ A  +DE G+     M  YG+G+SR+VAA IE N DE GI WP ++APF +++I +  H S+ V+  A K Y DL  K  +VL DD   RPG  F   +LIG PH II
Sbjct:   61 MNKAGALEVLMPCIQPAHLWMESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKAYINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFK----------TLKDNPDIDVDEIKSW---------------------YAAAEEKHDINKLPISE----------------QEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPVEMSSYGIGISRLVAAIIEANCDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAA-KAYKDLSDKNIEVLYDDTEARPGSKFATHDLIGSPHQII 393          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|75536572|sp|Q4ULX2.1|SYP_RICFE (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 318.931 bits (816), Expect = 4.123e-105
Identity = 168/381 (44.09%), Postives = 226/381 (59.32%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+KAGALE L+P +QPA LW ESGR+++YG E+L+FQDRH      GPT+E++IT+I +++I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDI+E+     Y++MYQAY N F  LG+    V ADNG IGGN SHEFH++A++GE  I YNK              L      D  +I  +                     Y  A  + D      ++                + I+  KGIEVGHIF +G KYS +++A  +DE G+     M  YG+G+SR+VAA IE N +E GI WP  +APF +++I +  H S+ V+  A K+Y +L A+  +VL DD   R G  F   +LIG P+ II
Sbjct:   61 MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYQAYINTFRDLGVFAIPVIADNGPIGGNLSHEFHIIAETGESTIYYNKRFK----------TLKDNPNIDVEEIKSW---------------------YAAAEEKHDVNKLPISE----------------QEITSSKGIEVGHIFYIGSKYSVNMNALINDEHGKLTPVEMSSYGIGISRLVAAIIEANCNEKGIIWPSNVAPFKVSLINLNIHDSKCVELAA-KVYKELSAQNIEVLYDDTEARAGSKFATHDLIGSPYQII 393          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|254782927|sp|C0R3N5.1|SYP_WOLWR (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 318.931 bits (816), Expect = 4.631e-105
Identity = 164/381 (43.04%), Postives = 227/381 (59.58%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            MDK+GA+E L+P VQPA LW+ESGR++DYG E+LR +DRH+ D   GPTHEEV T+++++ ++SY+ LPL LYQIQ KFRDE+RPR+G+MRGREF+MKDAYSFD+D +   + Y+ MY+ Y  IF R+GL    V AD G IGGN SHEFH+LA++GE  + Y+   +     E V +       AD                                               ++ D E CP   + E +++ KGIE+GHIF  G KYS+ + A  + + G+ V+ HMG YG+GVSR+V A IE  +D+ GI WPE++APF I +I +    +E     ADK+Y  L++   +VL DD     G+ F  M+LIG+P  II
Sbjct:   61 MDKSGAIEVLMPCVQPANLWRESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEVATDLIRDVVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDAYSFDVDYEGALNSYNLMYKTYIKIFKRMGLTPIGVRADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDVESLKSIYAVAD-----------------------------------------------DMHDPETCP--ISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHMGSYGIGVSRLVGAIIEAFHDDKGIIWPESVAPFRIGLINLQTKVTE----AADKIYKALKSD--EVLYDDTEGSVGVKFSRMDLIGLPWQII 386          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|114152245|sp|Q1RIC9.1|SYP_RICBR (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS >gi|166980150|sp|A8GU77.1|SYP_RICB8 RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 318.546 bits (815), Expect = 8.184e-105
Identity = 168/381 (44.09%), Postives = 230/381 (60.37%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            MDKAG LE L+P +QPA LW ESGR+++YG E+L+FQDRH      GPT+E+++T+I +N+I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDIDE++    Y++MY+AY N F  LG+    V ADNG IGG  SHEFH++A++GE +I Y+K              L      D  +I  +                     Y  A  + DA   S+                  + I+  KGIEVGHIF +G KYS +++A  +DE G+     M  YG+G+SR+VAA IE N+D  GI WP A+APF I++I +  H S+ ++  A+++Y +L A+  +VL DD + R G  F   +LIG P+ II
Sbjct:   61 MDKAGCLEVLMPCIQPAHLWVESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMVTDIFRNNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDIDEESAVKTYNQMYKAYINTFRDLGVFAVPVIADNGPIGGKLSHEFHIIAETGESNIYYDKRFK----------TLKDNPDIDIEEIKSW---------------------YAAAEEKHDASKLSS-----------------DKEITSSKGIEVGHIFYIGTKYSVNMNALINDEHGKLTPIEMSSYGIGISRLVAAIIEANSDAKGIIWPIAVAPFKISLINLNIHDSKCLE-LAERVYNELLAQNIEVLYDDTDVRAGSKFATHDLIGSPYQII 392          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|81854111|sp|Q92I92.1|SYP_RICCN (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS >gi|259511616|sp|C3PNA1.1|SYP_RICAE RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 317.39 bits (812), Expect = 1.746e-104
Identity = 169/381 (44.36%), Postives = 225/381 (59.06%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+KAGALE L+P +QPA LW ESGR+++YG E+L+FQDRH      GPT+E++IT+I +++I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDI+E+     Y++MY+AY N F  LG+    V ADNG IGGN SHEFH++A++GE  I Y+K                  T  D   I V    S                      W   A+     +   + + E+             KGIEVGHIF +G KYS +++A  +DE G+     M  YG+G+SR+VAA IE N DE GI WP ++APF +++I +  H S+ V+  A+  Y +L  K  +VL DD   RPG  F   +LIG PH II
Sbjct:   61 MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKAYINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFK---------------TLKDNPDIDVEEIKS----------------------WYAAAEEKYEVNKLPISEQEITS----------SKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEANCDEKGIIWPSSVAPFKVSLINLNIHDSKCVE-LAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQII 393          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|226701316|sp|B4RBZ9.1|SYP_PHEZH (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 317.775 bits (813), Expect = 2.166e-104
Identity = 169/381 (44.36%), Postives = 237/381 (62.20%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            MD+AGA+E L+P +Q A+LW+ESGR++DYG E+LR +DRH+RD   GPT EEVIT+I +  I+SY+ LP NLY IQ KFRDE RPRFG+MRGREF+MKDAYSFDIDE A +  Y++M+ AY N++ RLGL    V AD G IGG+ SHEF +LAD+GE  +  ++      ++ ++ AP P     D    P+    + +  +        S + +  A +E    T +  D R                    +GIEVGHIF  G+KYS+ ++A  +   G +   HMG YGVGVSR+V A IE ++DE GI WP+++APF   ++ +   ++  V A A++ Y  LQA G + LLDDR+ERPG  F  ++L+G+P  +I
Sbjct:   61 MDRAGAIEVLMPTLQLADLWRESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRAYIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDAYSFDIDEAAARRAYNRMFCAYLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHR------DLVEMGAPGPDID-WDGDLEPLVQQRTRLYAA--------SDEMHDQARFE----TEAPEDKR-----------------MTARGIEVGHIFYFGEKYSRPMNAKVAGPDGAERFVHMGSYGVGVSRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAA-VDAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVPWQLI 404          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|259511617|sp|C4K2D7.1|SYP_RICPU (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 317.005 bits (811), Expect = 2.875e-104
Identity = 169/381 (44.36%), Postives = 225/381 (59.06%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+KAGALE L+P +QPA LW ESGR+++YG E+L+FQDRH      GPT+E++IT+I +++I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDI+E+     Y++MY+AY N F  LG+    V ADNG IGGN SHEFH++A++GE  I Y+K                  T  D   I V    S                      W   A+     +   + + E+             KGIEVGHIF +G KYS +++A  +DE G+     M  YG+G+SR+VAA IE N DE GI WP ++APF +++I +  H S+ V+  A+  Y +L  K  +VL DD   RPG  F   +LIG PH II
Sbjct:   61 MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKAYINAFRDLGVFAIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFK---------------TLKDNPDIDVEEIKS----------------------WYAAAEEKYEVNKLPISEQEITS----------SKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEANCDEKGIIWPSSVAPFKVSLINLNIHDSKCVE-LAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQII 393          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|166980152|sp|A8GRW1.1|SYP_RICRS (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS >gi|189083508|sp|B0BXB9.1|SYP_RICRO RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 316.62 bits (810), Expect = 4.387e-104
Identity = 167/381 (43.83%), Postives = 225/381 (59.06%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+KAGALE L+P +QPA LW ESGR+++YG E+L+FQDRH      GPT+E++IT+I +++I+SY+ LP NLY IQ KFRDEIRPRFG+MRGREF+MKDAYSFDI+E+     Y++MY+AY N F  LG+    V ADNG IGGN SHEFH++A++GE  I Y+K              L      D  +I                            +W   A+     +   + + E+             KGIEVGHIF +G KYS +++A  +DE G+     M  YG+G+SR+VAA IE N DE GI WP ++APF +++I +  H S+ V+  A+  Y +L  K  +VL DD   RPG  F   +LIG PH II
Sbjct:   61 MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKAYINAFRDLGVFAIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKI----------LKDNPDIDVEEI---------------------------KSWYAAAEEKYEVNKLPISEQEITS----------SKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEANCDEKGIIWPSSVAPFKVSLINLNIHDSKCVE-LAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQII 393          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|81831258|sp|Q73GW6.1|SYP_WOLPM (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 312.768 bits (800), Expect = 9.081e-103
Identity = 160/381 (41.99%), Postives = 225/381 (59.06%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+K+GA+E L+P VQPA LW+ESGR++DYG E+LR +DRH+ D   GPTHEE+ T+++++ ++SY+ LPL LYQIQ KFRDE+RPR+G+MRGREF+MKDAYSFD+D +   + Y+ MY+ Y  IF R+G     V AD G IGGN SHEFH+LA++GE  + Y+   +     E + +       AD                                               ++ D E CP   + E +++ KGIE+GHIF  G KYS+ + A  + + G+ V+ HMG YG+GVSR+V A IE  +D+ GI WPE +APF I +I +    +E     ADK+Y  L++   +VL DD     G+ F  M+LIG+P  II
Sbjct:   61 MNKSGAIEALMPCVQPASLWRESGRYDDYGKEMLRIKDRHEEDMLFGPTHEEIATDLIRDVVKSYKDLPLCLYQIQWKFRDEVRPRYGVMRGREFLMKDAYSFDVDYEGALNSYNLMYKTYIKIFKRMGFTPIGVGADTGPIGGNLSHEFHILANTGESTLYYDNKFSELLESEDIESLKSIYAVAD-----------------------------------------------DMHDPETCP--ISQEQLNVSKGIEIGHIFYFGDKYSKPMKASVTSQDGKNVNIHMGSYGIGVSRLVGAIIEAFHDDKGIIWPEEVAPFRIGLINLQTKVTE----AADKIYKALKSD--EVLYDDTEGSVGVKFSRMDLIGLPWQII 386          

BLAST of prolyl-trna synthetase vs. SwissProt
Match: gi|189083491|sp|B0SZ28.1|SYP_CAUSK (RecName: Full=Proline--tRNA ligase; AltName: Full=Prolyl-tRNA synthetase; Short=ProRS)

HSP 1 Score: 313.153 bits (801), Expect = 1.297e-102
Identity = 167/381 (43.83%), Postives = 229/381 (60.10%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            MD+AGA+E L+P +Q A+LW+ESGR++DYG E+LR  DRHKRD   GPT EEV+T+I +  ++SY+ LP NLY IQ KFRDE RPRFG+MRGREF+MKDAYSFD+D +  +  Y++M+ AY N+F R+GL    + AD G IGG+ SHEF VLA++GE  +  +K      ++ ++ AP P         I  Y D   +V                      + +T   A   E+ D         G+ +S  +GIEVGHIF  G KYS+ + A  +   G+ V   MG YGVGVSR++ A IE ++DE GI WPE++APFD+ +I +    +   +A  +  Y  LQA G DVL DD + R G  F  M+LIG+P  +I
Sbjct:   61 MDRAGAIELLMPTIQLADLWRESGRYDDYGDEMLRITDRHKRDLLYGPTAEEVVTDIFRASVKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDAYSFDLDAEGARKAYNRMFVAYLNLFSRMGLKAVPMRADTGPIGGDLSHEFIVLAETGESAVFCHK------DLVEMAAPGP--------DIDWYGDLQPLV----------------------NERTALYAATEEMHDEAAFAALPEGDRLS-ARGIEVGHIFSFGTKYSEPMKALVTGPDGKDVPVQMGSYGVGVSRLLGAIIEASHDEGGIIWPESVAPFDVGVINMRQGDAACDEAC-ETAYKALQAAGRDVLYDDNDTRGGAKFATMDLIGVPWQLI 403          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|495763490|ref|WP_008488069.1| (proline--tRNA ligase [Idiomarina xiamenensis] >gi|407215056|gb|EKE84897.1| prolyl-tRNA ligase [Idiomarina xiamenensis 10-D-4])

HSP 1 Score: 441.81 bits (1135), Expect = 1.203e-149
Identity = 218/407 (53.56%), Postives = 276/407 (67.81%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEK-----VPAPLPTTTR----------ADASQIPVYADHSV---------IVMSDFICGANVSGKHYTGANWERDAKT--PSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M  AGALE L+PMVQPA+LW+ESGRW  YGPELLR QDR++R+F LGPTHEEVI  + + +I SY+QLPLNL+QIQTKFRDEIRPRFG+MR REFIMKDAYSF + E+++Q  YDKMYQAYCNIF RLGLN+ AV AD GSIGGN SHEF VLA SGED+IAY+ + +YAAN+E       P+ +    R           D  ++ + ADH +          V +      N  G+ Y   +   +  T   + ADIR VV G+  P      T++I +GIEVGHIFQLG+KYS+++      E+G+     MGCYG+G+SRIVAAAIEQ++D+ GI WP  +APF + II +  HKS  V+  A+ +Y  LQ  G +VL DDR ERPG+MF DMEL+GIPH I+
Sbjct:   61 MQAAGALEVLMPMVQPADLWQESGRWEAYGPELLRLQDRNQREFVLGPTHEEVIGELARKEISSYKQLPLNLFQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHLSEESLQQTYDKMYQAYCNIFTRLGLNFRAVIADTGSIGGNQSHEFQVLAKSGEDEIAYSDSSDYAANVEMAEALVTPSDIKEIQRIAVQASAESDVDYVELVLRADHQLNSVKAEKLSAVAAPLQRLENPQGQLYRIVDRSAEGLTDADTVADIRNVVSGDPSPCGQG--TLAIARGIEVGHIFQLGKKYSEAMKIGVLSETGKHETLTMGCYGIGISRIVAAAIEQHHDDRGICWPAPLAPFQVVIIPMNMHKSARVQEVAETLYQQLQNAGIEVLFDDRKERPGVMFADMELVGIPHQIV 465          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|1149057160|ref|WP_077641094.1| (proline--tRNA ligase [Salinivibrio sp. IB574] >gi|1146170565|gb|OOF20257.1| proline--tRNA ligase [Salinivibrio sp. IB574])

HSP 1 Score: 439.499 bits (1129), Expect = 4.772e-149
Identity = 212/396 (53.54%), Postives = 273/396 (68.94%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQI---------------PVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+K+GA+ETL+P+VQPA+LW+E+GRW  +GPELLR  DRH+R F LGPTHEEVITN+V+N+I SY+QLP+NLYQIQTKFRDE+RPRFG+MR REFIMKDAYSFDID+  M+  Y+KM  AYC IFDR+GL+Y AV AD G+I      +   L   G+ ++   K    A N+ +V APL   T  +   +               P   D SV VM+DF  GAN+  KHY G NW RD +    AD+R VV+G+  P      T+   +GIEVGHIFQLG  YS+ + A    + G+     MGCYG+GV+R+VAAAIEQN+DENGI WP+A+APF + I+ +  HKSE V+  A+K+YADL A G +VL DDR ERPG+MF D+EL+G+PH I+
Sbjct:   61 MNKSGAVETLMPVVQPADLWQETGRWEKFGPELLRIADRHERPFVLGPTHEEVITNLVRNEINSYKQLPINLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFDIDKAGMEQSYEKMRAAYCAIFDRMGLDYRAVLADTGAI---VDADLIALIIRGDHELNEVK----AENLPEVAAPLTFATEEEIRALVGAGPGSLGPVNLPAPFIVDRSVAVMNDFGAGANIDDKHYFGINWGRDVELGQVADLRNVVEGDPSPCGKG--TLMFKRGIEVGHIFQLGNNYSEKMGAGVLGQDGKNTTLEMGCYGIGVTRVVAAAIEQNHDENGIIWPDAIAPFTVAIVPMNMHKSERVREAAEKLYADLTAAGIEVLFDDRKERPGVMFSDIELVGVPHTIV 447          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|1278366005|gb|PIZ04578.1| (proline--tRNA ligase [Gammaproteobacteria bacterium CG_4_10_14_0_8_um_filter_38_16] >gi|1278479673|gb|PJA03698.1| proline--tRNA ligase [Gammaproteobacteria bacterium CG_4_10_14_0_2_um_filter_38_22] >gi|1278599933|gb|PJB11352.1| proline--tRNA ligase [Gammaproteobacteria bacterium CG_4_9_14_3_um_filter_38_9])

HSP 1 Score: 394.045 bits (1011), Expect = 4.821e-132
Identity = 194/381 (50.92%), Postives = 243/381 (63.78%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            M+ AGALE L+P +QPAELW E+ RW  YG ELL+ +DRH+RDFC GPTHEEV+T +++N++ SY+QLP+N+YQIQTKFRDEIRPRFG+MR REF+MKDAYSF +D  ++Q  Y+ MYQAY  IF RLGL++ AV AD G IGG  SHEF VLA+SGED + Y+   +YA                                                AN ER         ++E   G+  P       +   +GIEVGH+FQLG KYS+S++     E G+ +H  MGCYGVGVSRIVAA IEQN+DE GI WPE MAPF I II +  HK+  V+  A K+Y  L A GY+VLLDDR ERPG+MF DM+LIGIPH ++
Sbjct:   61 MNHAGALEILMPAIQPAELWMETDRWEKYGNELLKIKDRHERDFCFGPTHEEVVTYLMRNELSSYKQLPINVYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHVDMASLQKTYEVMYQAYTAIFTRLGLHFRAVLADTGQIGGAFSHEFQVLAESGEDVVVYSDGSDYA------------------------------------------------ANIERAENLEKTQPVKE---GDASPDGKG--KLKFARGIEVGHVFQLGDKYSKSMNLTVLGEDGKALHPQMGCYGVGVSRIVAACIEQNHDEKGICWPEPMAPFQIVIIPMQMHKAYRVRDAAMKLYDKLTAAGYEVLLDDRRERPGVMFADMDLIGIPHRVV 388          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|1176238344|gb|OQY46921.1| (proline--tRNA ligase [Desulfobacteraceae bacterium 4572_87])

HSP 1 Score: 337.421 bits (864), Expect = 8.184e-109
Identity = 182/402 (45.27%), Postives = 248/402 (61.69%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADA------SQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIR---EVVDGELCPGATNGETISICK--------GIEVGHIFQLGQKYSQSIDAYCSDES-----GEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHI 380
            M++AGA+E L+P VQPAELW+ESGRW  YG ELLRF+DRH R+ C+GPTHEEVIT++V+ +I SY+Q+P+NLYQIQTKFRDEIRPRFG+MRGREFIMKDAYSFD+DE      Y+ MY+AY  IF R GL +  VEAD+G+IGG+ SHEF VLA+SGED I    A +YAAN+E+           D        ++      ++  +++F+    +S K        R  + P A  IR   E+ D +L   A + +++ +          G  +G    +G K     D            G +   H     + + R VAAAIEQN+D++GI +P  +APF++TI+ +  H  E+V A A K+Y DL + G DVLLDDR+ER G+ FKD +L+GIP  +
Sbjct:   61 MNRAGAIEVLMPSVQPAELWQESGRWEYYGRELLRFKDRHDREACMGPTHEEVITDLVRKEIHSYKQMPINLYQIQTKFRDEIRPRFGIMRGREFIMKDAYSFDVDEDGANKSYEAMYRAYERIFKRCGLKFRPVEADSGTIGGSHSHEFMVLAESGEDQIVNCTACDYAANLERAEVKETEVASPDTDGFEALGRVDTPDQRTIDEVAEFL---KISSKQLVKTLVYRVDEEPVAVLIRGDHELNDAKL-KNALDAQSVEMASERVVKEATGAPLGFAGPVGLKIKVVADNAVKAMKNFVVGGNEKDLHFQNANLDIGRTVAAAIEQNHDKDGIVFPVPVAPFEVTILPLQMHNEEVV-AAASKIYDDLLSMGIDVLLDDRDERAGVKFKDADLLGIPVRV 457          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|495517153|ref|WP_008241798.1| (proline--tRNA ligase [Sulfurovum sp. AR] >gi|385345210|gb|EIF51922.1| prolyl-tRNA synthetase [Sulfurovum sp. AR])

HSP 1 Score: 336.265 bits (861), Expect = 2.875e-108
Identity = 182/412 (44.17%), Postives = 239/412 (58.01%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKV---PAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGAN---------WERDAKTPSA-------------------ADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            +DKAG  E  L  V PA LW+ESGR   YG ELLRF+DR + DF LGPTHEE++ N+V+  ++SY+QLPLN+YQI  KFRDEIRPRFGLMRGREF+MKDAYSF    + MQ  +  M + Y  IF RLGL +  V+AD+G+IGG+ S EF VLADSGED I      +Y ANIE     P     +T      +    D  V  +  F   A+V  +     N         +E +A   +                    ADI  V +G+ C   +   ++   KGIE GHIFQLG +YS+ + A   DE+G+     MG YG+GVSR++AA IEQN+D+ G  W +  APFD+ II +   K +      +++Y  + AKG DVLLDDR +R G   KD ELIG+PH ++
Sbjct:   62 LDKAGCQEVSLAFVTPAALWEESGRLEKYGKELLRFKDRKENDFILGPTHEEMMVNLVRQTVKSYKQLPLNVYQINLKFRDEIRPRFGLMRGREFLMKDAYSFHTSTEDMQREFALMEETYKKIFTRLGLEFRVVDADSGAIGGSGSKEFMVLADSGEDTIVVCNECDYGANIEAAVAKPKAYDASTEIKVIAMKALYDEGVSKVVHFALPASVELQDVKACNAVNANDLVEYEEEAAIVTLVVDTALEGIDDLTAEEKIYADISAVQEGDACASCSG--SLRYTKGIEAGHIFQLGTRYSEPLAANFLDENGKSQPMVMGTYGIGVSRLLAAIIEQNHDDKGCIWTKESAPFDLQII-VSNIKDDAQVTLGEQLYEAMLAKGVDVLLDDRKDRFGAKIKDYELIGVPHAVV 470          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|1270448908|gb|PHS41296.1| (proline--tRNA ligase [Sulfurovum sp.])

HSP 1 Score: 332.798 bits (852), Expect = 5.030e-107
Identity = 184/410 (44.88%), Postives = 236/410 (57.56%), Query Frame = 0

Query:    1 MDKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPT---TTRADASQIPVYADHSVIVMSDFI-----CGANVSGKHYTGANWERDAKTPSA---------------------ADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            +DKAG  E  L  V PA LW+ESGR   YG ELLRF+DR   DF LGPTHEE++ N+V+  ++SY+QLPLNLYQI  KFRDEIRPRFGLMRGREF+MKD YSF    + +Q  +  M + Y  IF RLGL +  V+AD+G+IGG+ S EF VLADSGED I       Y ANIE   A   +   +T      +    D  V  + +F+         V   +   AN   D + P+                      ADI  V +G+ C     G  +   KGIE GHIFQLG +YS+ + A   DE+G+     MG YG+GVSR++A  IEQN+D+ G  W +  APFD+ II I   K E   A  +K+YA +  KG DVLLD+R +R G   KD ELIG+PH ++
Sbjct:   62 LDKAGCQEVSLSFVTPAALWEESGRLEKYGKELLRFKDRKNNDFILGPTHEEMMVNMVRQSVKSYKQLPLNLYQINLKFRDEIRPRFGLMRGREFLMKDGYSFHASTEDLQREFALMEETYKKIFTRLGLEFRVVDADSGAIGGSGSKEFMVLADSGEDTIVVCPDCEYGANIEAAVAQSKSYDASTEIKVVTMKALYDEGVTEIVEFVLEESELLEEVKACNSINANDLVDVENPNTVDRRMIDNNLKECTVYENPEYADISAVQEGDACTRC--GGALHYSKGIEAGHIFQLGTQYSEPLAANFLDENGKAQPMVMGTYGIGVSRLLAGIIEQNHDDKGCVWTKESAPFDLQII-ISNIKDEAQVALGEKLYAAMLEKGVDVLLDERKDRFGAKMKDYELIGVPHAVV 468          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|1111725609|gb|OJJ12160.1| (proline--tRNA ligase [Alphaproteobacteria bacterium AO1-B])

HSP 1 Score: 325.479 bits (833), Expect = 6.088e-105
Identity = 167/379 (44.06%), Postives = 240/379 (63.32%), Query Frame = 0

Query:    3 KAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            +AGA++ L+P +QPA+LW+ESGR++ YG E+LR  DRH+R+   GPT+EE+IT+I ++ +RSY+ LPLNLY IQ KFRDE+RPRFG+MRGREF+MKDAYSFD+D+++ +  Y++M+ AY   FDR+GL    ++AD G IGG+ SHEF +LA++GE ++  +K+L        +  P+P      A     ++D S IV S                 W     TP AA   + +  E   GA + +T    +GIEVGHIF  G KYS ++ A  +   G +V  HMG YGVGVSR++ A IE N+DE+GI WP+++APF + II +     +L     + +YA L+A G +VL DD + R G  F  M+LIG+P  ++
Sbjct:   63 RAGAVQLLMPTIQPADLWRESGRYDAYGKEMLRITDRHEREMLFGPTNEEMITDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGIMRGREFLMKDAYSFDLDQESARHAYNRMFVAYLRTFDRMGLKAIPMKADTGPIGGDLSHEFIILAETGESEVFLDKSL--------LEMPIP------AKDTDFWSDLSPIVES-----------------W----TTPYAAT--DEMHDETAFGALSEDTRVSARGIEVGHIFYFGTKYSDAMGAKVATAEGTEVPVHMGSYGVGVSRLLGALIEANHDEDGIIWPKSVAPFHVGIINM-RPGDDLTDPVCEDLYAKLEAAGIEVLYDDTDSRAGAKFATMDLIGLPFQVV 403          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|946930082|ref|WP_055852023.1| (proline--tRNA ligase [Aurantimonas sp. Leaf443] >gi|945325292|gb|KQT82739.1| proline--tRNA ligase [Aurantimonas sp. Leaf443])

HSP 1 Score: 324.709 bits (831), Expect = 1.085e-104
Identity = 168/380 (44.21%), Postives = 236/380 (62.11%), Query Frame = 0

Query:    2 DKAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            D++GA+E L+P +Q A+LW+ESGR++DYG E+LR +DRH+RD   GPT+EE++T+I ++ +RSY+ LPLNLY IQ KFRDE+RPRFG+MR REF+MKDAYSFDID+++ +  Y++M+ AY   F+R GL    + AD G IGG+ SHEF +LA +GE  +  ++      +   +P P      ADA            V++D +              W     TP AA   + +  E    A   +     +GIEVGHIF  G KYS +++A  +   G+ V  HMG YG+G SR++AA IE  +DENGI WP+++APFDI +I +    +E  KA  D +YA LQA G+DVL DD + R G  F  M+LIG+P  +I
Sbjct:   62 DRSGAVELLMPTIQSADLWRESGRYDDYGKEMLRIEDRHERDLLFGPTNEEMVTDIFRSSVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDIDQESARIAYNRMFVAYLRTFERCGLKAIPMRADTGPIGGDLSHEFIILASTGESQVYCHR------DFLDLPVPPSGVDFADAQ-----------VLADIVSA------------W----TTPYAAT--DEMHDEAAWNAVPADAQLAARGIEVGHIFYFGTKYSAAMNAVVTGPDGKDVPVHMGSYGIGPSRLIAAIIEAFHDENGIVWPKSIAPFDIGLINMKPGDAECDKA-CDDLYAKLQAAGFDVLYDDEDTRAGAKFATMDLIGLPTQVI 405          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|495469118|ref|WP_008193807.1| (proline--tRNA ligase [Labrenzia alexandrii] >gi|222439005|gb|EEE45684.1| prolyl-tRNA synthetase [Labrenzia alexandrii DFL-11])

HSP 1 Score: 324.709 bits (831), Expect = 1.387e-104
Identity = 167/379 (44.06%), Postives = 238/379 (62.80%), Query Frame = 0

Query:    3 KAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            +AGA++ L+P +QPA+LW+ESGR++ YG E+LR  DRH+R+   GPT+EE+IT+I ++ +RSY+ LPLNLY IQ KFRDE+RPRFG+MRGREF+MKDAYSFD+D++  +  Y++M+ AY   FDR+GL    ++AD G IGG+ SHEF +LA++GE ++  +K+L        +  P+P      A     ++D S IV S                 W     TP AA   + +  E   GA   +T    +GIEVGHIF  G KYS ++ A  +   G +V  HMG YGVGVSR++ A IE N+DE+GI WP+++APF + II +     +L     + +YA L+A G +VL DD + R G  F  M+LIG+P  ++
Sbjct:   63 RAGAVQLLMPTIQPADLWRESGRYDAYGKEMLRITDRHEREMLFGPTNEEMITDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGIMRGREFLMKDAYSFDLDQETARHAYNRMFVAYLRTFDRMGLKAIPMKADTGPIGGDLSHEFIILAETGESEVFLDKSL--------LEMPIP------AKDTDFWSDLSPIVES-----------------W----TTPYAAT--DEMHDEAAFGALEEDTRVSARGIEVGHIFYFGTKYSDAMGAKVATAEGTEVPVHMGSYGVGVSRLLGALIEANHDEDGIIWPKSVAPFHVGIINM-RPGDDLTDPVCEDLYAKLEAAGIEVLYDDTDSRAGAKFATMDLIGLPFQVV 403          

BLAST of prolyl-trna synthetase vs. nr
Match: gi|944184251|ref|WP_055672863.1| (proline--tRNA ligase [Labrenzia alexandrii] >gi|933282967|emb|CTQ73237.1| Proline--tRNA ligase [Labrenzia alexandrii])

HSP 1 Score: 324.324 bits (830), Expect = 2.117e-104
Identity = 167/379 (44.06%), Postives = 238/379 (62.80%), Query Frame = 0

Query:    3 KAGALETLLPMVQPAELWKESGRWNDYGPELLRFQDRHKRDFCLGPTHEEVITNIVKNDIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFDIDEQAMQDVYDKMYQAYCNIFDRLGLNYSAVEADNGSIGGNSSHEFHVLADSGEDDIAYNKALNYAANIEKVPAPLPTTTRADASQIPVYADHSVIVMSDFICGANVSGKHYTGANWERDAKTPSAADIREVVDGELCPGATNGETISICKGIEVGHIFQLGQKYSQSIDAYCSDESGEKVHYHMGCYGVGVSRIVAAAIEQNNDENGITWPEAMAPFDITIITIGYHKSELVKATADKMYADLQAKGYDVLLDDRNERPGIMFKDMELIGIPHHII 381
            +AGA++ L+P +QPA+LW+ESGR++ YG E+LR  DRH+R+   GPT+EE+IT+I ++ +RSY+ LPLNLY IQ KFRDE+RPRFG+MRGREF+MKDAYSFD+D++  +  Y++M+ AY   FDR+GL    ++AD G IGG+ SHEF +LA++GE ++  +K+L        +  P+P      A     ++D S IV S                 W     TP AA   + +  E   GA   +T    +GIEVGHIF  G KYS ++ A  +   G +V  HMG YGVGVSR++ A IE N+DE+GI WP+++APF + II +     +L     + +YA L+A G +VL DD + R G  F  M+LIG+P  ++
Sbjct:   63 RAGAVQLLMPTIQPADLWRESGRYDAYGKEMLRITDRHEREMLFGPTNEEMITDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGIMRGREFLMKDAYSFDLDQETARHAYNRMFVAYLRTFDRMGLKAIPMKADTGPIGGDLSHEFIILAETGESEVFLDKSL--------LEMPIP------AKDTDFWSDLSPIVES-----------------W----TTPYAAT--DEMHDEAAFGALAEDTRVSARGIEVGHIFYFGTKYSDAMGAKVATAEGTEVPVHMGSYGVGVSRLLGALIEANHDEDGIIWPKSVAPFHVGIINM-RPGDDLTDPVCEDLYAKLEAAGIEVLYDDTDSRAGAKFATMDLIGLPFQVV 403