BLAST of tiptop vs. L. salmonis genes
Match: EMLSAG00000008256 (supercontig:LSalAtl2s:LSalAtl2s49:154084:157732:-1 gene:EMLSAG00000008256 transcript:EMLSAT00000008256 description:"maker-LSalAtl2s49-augustus-gene-1.6")

HSP 1 Score: 417.157 bits (1071), Expect = 1.955e-127
Identity = 327/722 (45.29%), Postives = 387/722 (53.60%), Query Frame = 0

Query:  122 LFKSNPLLSFNPALLSLNPSLYAVQLAQLQAAQLLSQSVGLHKSNPDLAASIAEEEFNMRKRKLAMQDEEAEMGAGVLNLSKRSPLGSPKMRAESPLDLSGNGGGGRRPSDLMSPAKAPKLDIPPWATGAF-PFLNPFLAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAKG----------------------------------SANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSA----------------------DSQTSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNEDTPGKISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAGP----DSNMSILQRLGIDEGV--DYSKPLVDPMAMFRP 780
            LFK NPL  FNPALLSLNPSLYAVQLA LQAAQLLSQ+      +  L+          RKRK+    E+  +G      SKR P+ SPK   ESPLDL+ +   G       SP K     + P+A  A  PF +PFL            G  P  L P + A SS   +S   +    NPLERMSEI KG                                    N    GPR SAWQSQWI+RGPE +KDIF+CVWCKD+F SLQ LT HMKETKHFG  IP S        +R      ++S +T    H + P     +TT +M+            + +  +S  + RDILKEQLP+PRKLVRGQDVWLGRGEQQTKDILKCMWCG+SFRSLDLMTKHMQETKHYTKVISQEQL SWKS                            +Q+HVN+VLTCKVCD ++STL++LSDHMVKNNHY ES+  P  S  ++SP                                          +KEKRKKSLPVRKLLELERAQQE       G +   GKI+CEKC EKI + +F  HI  C+G      S PS   L R+ PS+S   STP   +PS     +  DHQ      ++ KSILGSLEKMVQSNFG   K       D N+SILQRLGIDE +  D+S+P    +  FRP
Sbjct:  112 LFK-NPL--FNPALLSLNPSLYAVQLAHLQAAQLLSQASSSSSDDSPLS----------RKRKML---EDNALGG----TSKR-PM-SPK---ESPLDLTKDVAKGT-----FSPGK-----LDPFAAAALSPFFSPFL------------GARPEMLLPGTGASSSSDSNSFFKN----NPLERMSEIIKGPHQPSYSHQNTSNNNNVLSNHHQQHSPSSLPSGGGTNKNTSGPRHSAWQSQWINRGPENSKDIFKCVWCKDAFSSLQDLTDHMKETKHFGPQIPSSSPPQISPGLR------VSSKSTG---HQAPPPPTPTQTTSLMS-----------TSPSPLLSSPKTRDILKEQLPMPRKLVRGQDVWLGRGEQQTKDILKCMWCGQSFRSLDLMTKHMQETKHYTKVISQEQLSSWKSGGGGNESSVPISSPSPNSASSSSQQHQHNQSHVNSVLTCKVCDSSFSTLRDLSDHMVKNNHYNESKSGPN-SRSVVSPPPPNLP----------------------------------PPSKEKRKKSLPVRKLLELERAQQEF---IISGIKSDEGKITCEKCSEKIPVSLFSSHIHLCIG------SGPS--DLLRRPPSSSAASSTPPSISPSSTKQNTVEDHQHENESDKQPKSILGSLEKMVQSNFGASKKKSTLPVVDGNLSILQRLGIDESIPTDFSRPNNAELHNFRP 716          

HSP 2 Score: 154.066 bits (388), Expect = 4.448e-38
Identity = 100/220 (45.45%), Postives = 118/220 (53.64%), Query Frame = 0

Query: 1079 TSKDEDQLANEQGRSKKKKSHPLAALQLLCDKTEK-----------------------TPHTPVTSTTMYGVPTDPGAILAFSWACNQAVVNDSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGSAGP---GNPHDKGGSPGGQNQGV--------------------------EGQMKEENAQSKFQKYSQLAKQLSCSTQP 1246
            TS +E+  + + GR K    +PLAALQ+LCDKTEK                       +   PV ++++Y   + PGAILAFSWACN A+VNDSLLKCPFCDTPFISKGAYRHHLSKMHFVK     D K  M      P S GP    +P++   +    N  V                              K ENAQSKFQKYSQLAKQLSCS+QP
Sbjct:  814 TSHNENTSSEDPGRKK----NPLAALQMLCDKTEKPKCSSSSLNNNGGHMPTVTSASPSSILPVGASSLY---SXPGAILAFSWACNXAIVNDSLLKCPFCDTPFISKGAYRHHLSKMHFVK-----DEKGIMGSPFYHPVSKGPEKSPSPNESSSTSSSMNNLVAPPPPLNNSNSNHHVNNNNNSASSPSSSSAKAENAQSKFQKYSQLAKQLSCSSQP 1021          

BLAST of tiptop vs. L. salmonis genes
Match: EMLSAG00000008257 (supercontig:LSalAtl2s:LSalAtl2s49:363225:366513:1 gene:EMLSAG00000008257 transcript:EMLSAT00000008257 description:"maker-LSalAtl2s49-augustus-gene-3.4")

HSP 1 Score: 304.294 bits (778), Expect = 2.150e-88
Identity = 206/509 (40.47%), Postives = 276/509 (54.22%), Query Frame = 0

Query:  282 SSAMSSPPPSSSVTSSENRNPLERMSEIAKGSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWK------------------SADSQTSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPP------HPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNEDTPGKISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAGPDSNMSI-LQRLGIDEGV 765
            ++ + SPP   S  SS   +PL++M+++ KG         R SAWQSQWI+RG E  KDIF+C WCK+ F S Q+LT HMKETKHFG S+PPS +     +     S+     +      +S P +     T+  AS+P   +++S   N SS  K+  RDILKEQLP+PRKLVRGQDVWLG+GE+QT++ILKCMWCGESFRSL++MTKHMQETKHYTKVISQ+Q+ SWK                  ++ S+ +    +N++L+CKVC+ ++ TLK+LSDHM+KNNH   S  P +P                                L N SPP                 KEKRKKSLPV+KLLELER+ ++           T  K  C+KCG     H+F +HIR C G +    SSP  +R    +P T  PV T +  + +  SH               + +ILGSLE+MV  NF    K  P  ++ I + + G DE +
Sbjct:  193 AAGLPSPPKRPSFESS---SPLDKMTDMTKGKQ-------RHSAWQSQWINRGEENAKDIFKCTWCKECFHSFQALTHHMKETKHFGGSLPPSSSPGPFKNTSSSPSLSSGPSSITALPQISPPKSNPPPNTNPSASSPLLDTNSSYEMNCSSGPKK--RDILKEQLPIPRKLVRGQDVWLGKGEEQTRNILKCMWCGESFRSLEVMTKHMQETKHYTKVISQDQISSWKSNNQVSNGSSSSSVPPAVTSPSKDNKDQPMNSILSCKVCETSFQTLKDLSDHMIKNNHCINS--PKSP--------------------------------LKNSSPPASVTSVSNNNNNFTFPPKEKRKKSLPVKKLLELERSYEK----------GTTMKFPCDKCGISFPFHLFAEHIRNCNGLVSRASSSPEQKRSESLSPPTIAPVLT-NRMSSTRKSHDGG------------DSNILGSLEEMVSGNF----KGLPRDDVVIPVIKEGQDEDI 628          

HSP 2 Score: 103.219 bits (256), Expect = 1.981e-22
Identity = 71/168 (42.26%), Postives = 87/168 (51.79%), Query Frame = 0

Query: 1099 HPLAALQLLCDKTEKTPHTPVTSTTMYGVPTDPGAI--------LAFSWACNQAVVNDSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSA---PDHKMFMAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQM---------KEENAQSKFQKYSQLAKQLSCSTQP 1246
            +PL ALQ+LCDK EK                +P  +        + F W+ +     ++L KCPFC+TPF+SKGAYR+HLSKMHFVKD S     D    MA A G P    P  PH  GG  G    G+ G            EEN+ SKF+KYSQLAKQLS   +P
Sbjct:  659 NPLEALQMLCDKNEKQTQNFYGGGGGTPTGGNPHMLGSSSPXDSMVFPWSPSP----EALFKCPFCETPFVSKGAYRNHLSKMHFVKDPSVMMMMDPASIMALAAGIPSGFFP--PHHSGGGSGNVLGGLSGMQGPPVSTSPDFEENSTSKFEKYSQLAKQLSNQRKP 820          

BLAST of tiptop vs. SwissProt
Match: gi|37999961|sp|Q9U3V5.2|TIPT_DROME (RecName: Full=Protein tiptop)

HSP 1 Score: 312.383 bits (799), Expect = 2.585e-88
Identity = 229/630 (36.35%), Postives = 312/630 (49.52%), Query Frame = 0

Query:  217 PLDLSGNGGGGRRPSDLMSPA-KAP-------KLDIPP--WATGAFPFLNPFLAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAK-----------------------GSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIP-PSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTSSQN-------------------HVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELER--AQQEMSGQFK-MGNEDTPGKISCEKCGEKILMHIFVDHIRQCVG---PLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKT----------KAGPDSNMSILQRLGIDEGVDYSKPLVDPMAM 777
            PLDLS + G  +R   +  PA K P       K  +PP  W     P +NP+LA     +    GG+G   L P                SE    LE+M+E+++                       G+++N+ G  R SAWQS W+++G +  KD+F+CVWCK SF +L +LT HMKET+H G  IP P PT                         + +P AP        AS    +SS+S  +++S+ SK +   ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D +    N                    V+AVLTCKVCDQA+ +LKELS HM + +HYKES                                  S    G G+P       G  +  EKRKKSLPVRKLLELER  +   +    K + +     KI+CEKCG KI   +FV+HIR+C+G   P+   RS+  ++RL                  PSP           LG   E+  S+L +LE++++ +F ++T          +AG     SIL+RLGI++  DY+KPL+D  AM
Sbjct:  187 PLDLSVSTG--KRQESVSPPARKIPRSISADYKSPLPPGSW----MPPINPYLA----AVAAKTGGLGYSKLAP----------------SEASKALEKMTEMSRLETSPTAARSLGATSSVGAGVPAGASSNSGG--RHSAWQSHWLNKGADTAKDVFKCVWCKQSFSTLANLTAHMKETQHCGVQIPSPLPTG-----------------------GVGTPSAPPPTRLATSASNSACSSSSSSTSSSSNSSKSELNMLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSGDERERPTNTGVPSTSTAAPSSPSCTAPSVSAVLTCKVCDQAFGSLKELSTHMAQKSHYKES-----------------------------PAPSASPPAAGTGNPKR-----GRQNRNEKRKKSLPVRKLLELERSGSNSSLDSALKPLRDFAAATKITCEKCGSKIETALFVEHIRKCLGESIPIPPRRSNAGVDRL------------------PSPS----------LGLGAEKPPSVLNALEQLIEKSFESRTSRTMTHGGYSEAGTPLGASILKRLGIEDSSDYTKPLMDAQAM 703          

HSP 2 Score: 105.916 bits (263), Expect = 1.002e-21
Identity = 70/170 (41.18%), Postives = 99/170 (58.24%), Query Frame = 0

Query: 1093 SKKKKSHPLAALQLLCDKTE------KTPHTPVTSTTMYGVPTDPGA-ILAFSWACNQAVV---------NDSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGS---AGPGNPHDKGGSPGGQNQGVEGQMK--EENAQSKFQKYSQLAKQLS 1241
            ++K K+HPLAALQ LC+ T+      ++  +  +ST    +P+  G  ++AFSWACN+AV+         + S++KC +CDTPF SKGAYRHHLSK+HFVKD +  D     + A   P S   A P     +  + G Q       +   +E+ QSKF KY++LAKQLS
Sbjct:  852 AQKPKAHPLAALQKLCETTDPPSTGLRSASSAGSSTASATLPSANGNDLVAFSWACNEAVLSASNGGSAGDSSIIKCSYCDTPFASKGAYRHHLSKVHFVKD-AGEDSPRLKSPAVQSPRSMPLASPRRSASRSPATGSQQPPPSPTISPYDESPQSKFLKYTELAKQLS 1020          

BLAST of tiptop vs. SwissProt
Match: gi|73621300|sp|P22265.2|TSH_DROME (RecName: Full=Protein teashirt)

HSP 1 Score: 231.876 bits (590), Expect = 1.918e-61
Identity = 190/596 (31.88%), Postives = 265/596 (44.46%), Query Frame = 0

Query:  246 PWATGAFPFLNPFLAV----------------SSSGMVMSP---------GGVGPMGLP-------PTSSAMSSPPPSSSVTSSENRNPLERMSEIAKGSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQ-----QYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTSS---QNH----------VNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNEDT---PGKISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGT---------------KTKAGPDSNMSILQRLGIDEGVDYSKPLVD 773
            P A+   P +NP+LA                 S + +V+ P         GG   MG          T+   +S PP S V  + N+N           + + A    R + WQS W ++G      +FRCVWCK SFP+L++LTTHMK++KH G ++PP                    P+         P  P     H +         +SG+A+N S S       QYR       PLPRKLVRGQ+VWLG+G +Q   ILKCM CGESFRSL  MTKHMQET+HYT ++SQEQ +S KS ++  +S   ++H          ++AVLTCKVCD+A+++L +LS+HM KNNHY E        P + S G+                             P P          +KR+KSLPVRKLLE++      + Q    NE T            G+K    +F + +RQ +  +  P     +        + SP +          V  K+    +  G       S+L ++E+M  ++F T                TK       SIL+RLGIDE VDY+KPL+D
Sbjct:  228 PSASQLLPGVNPYLAAVAAANIFRAGQFPDWNSKNDLVVDPLEKMSDIVKGGASGMGTKEKMHSSKATTPQAASQPPKSPVQPTPNQNSESGGGSGGGAAGSGAVTKARHNIWQSHWQNKG--VASSVFRCVWCKQSFPTLEALTTHMKDSKHCGVNVPP----------------FGNLPSNNPQPQHHHPTPPPPPQNHNLRK------HSSGSASNHSPSANVKNAFQYRG--DPPTPLPRKLVRGQNVWLGKGVEQAMQILKCMRCGESFRSLGEMTKHMQETQHYTNILSQEQSISIKSGNANANSDAKESHNSLSSEESRTLSAVLTCKVCDKAFNSLGDLSNHMAKNNHYAE--------PLLQSAGARKR--------------------------PAP----------KKREKSLPVRKLLEMKGGSG--TTQEDHSNEKTSVQGKPGLGPGGGDKNDAALFAERMRQYITGVKAPEEIAKVAAAQLLAKNKSPEL----------VEQKNGGSAKAAGA-----SSVLSAIEQMFTTSFDTPPRHASLPASSPSNSSTKNTSPVASSILKRLGIDETVDYNKPLID 736          

BLAST of tiptop vs. SwissProt
Match: gi|81910749|sp|Q68FE9.1|TSH2_MOUSE (RecName: Full=Teashirt homolog 2; AltName: Full=SDCCAG33-like protein; AltName: Full=Zinc finger protein 218)

HSP 1 Score: 65.855 bits (159), Expect = 1.900e-9
Identity = 56/184 (30.43%), Postives = 83/184 (45.11%), Query Frame = 0

Query:  321 PRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPL-PRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVIS 503
            PR+ A+Q        E  + + +C++C DSF SLQ L+ HM +TKH+   +P                  +  P   ISS + +P    V       + P S  ST+G+  + S S Q+  ++   QLP   R   +    +  + E     ILKCM CG S  +L  +T HM  T H+ KV S
Sbjct:  259 PRKRAFQDM----DKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHY-QKVP------------------LKEPVPTISSKMVTPAKKRV----FDVNRPCSPDSTTGSLAD-SFSSQKSANL---QLPSNSRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTS 411          

BLAST of tiptop vs. SwissProt
Match: gi|108935911|sp|Q9NRE2.3|TSH2_HUMAN (RecName: Full=Teashirt homolog 2; AltName: Full=Ovarian cancer-related protein 10-2; Short=OVC10-2; AltName: Full=Zinc finger protein 218)

HSP 1 Score: 64.3142 bits (155), Expect = 6.130e-9
Identity = 53/183 (28.96%), Postives = 79/183 (43.17%), Query Frame = 0

Query:  321 PRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVIS 503
            PR+ A+Q        E  + + +C++C DSF SLQ L+ HM +TKH+   +P                  +  P   ISS + +P    V       + P S  ST+G+  +S  S++     L       R   +    +  + E     ILKCM CG S  +L  +T HM  T H+ KV S
Sbjct:  258 PRKRAFQDM----DKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHY-QKVP------------------LKEPVPTISSKMVTPAKKRV----FDVNRPCSPDSTTGSFADSFSSQKNANLQLSSN---NRYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTS 410          

BLAST of tiptop vs. SwissProt
Match: gi|158513307|sp|A5GFT6.1|TSH2_PIG (RecName: Full=Teashirt homolog 2)

HSP 1 Score: 61.6178 bits (148), Expect = 3.700e-8
Identity = 51/184 (27.72%), Postives = 74/184 (40.22%), Query Frame = 0

Query:  321 PRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTP-PSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVIS 503
            PR+ A+Q        E  + + +C++C DSF SLQ L+ HM +TKH+       P         P  S  M +P       ++ P +P   T       P P  +S   ++NN                   R   +    +  + E     ILKCM CG S  +L  +T HM  T H+ KV S
Sbjct:  259 PRKRAFQDM----DKEDAQKVLKCMFCGDSFDSLQDLSVHMIKTKHYQKVPLKEPV--------PTISSKMVTPAKKRVFDVNRPCSPDSTTGSFADPFPAPKNASLQLSSNN-------------------RYGYQNGASYTWQFEACKSQILKCMECGSSHDTLQQLTTHMMVTGHFLKVTS 411          

BLAST of tiptop vs. SwissProt
Match: gi|85683261|sp|Q6ZSZ6.2|TSH1_HUMAN (RecName: Full=Teashirt homolog 1; AltName: Full=Antigen NY-CO-33; AltName: Full=Serologically defined colon cancer antigen 33)

HSP 1 Score: 60.4622 bits (145), Expect = 8.593e-8
Identity = 45/172 (26.16%), Postives = 71/172 (41.28%), Query Frame = 0

Query:  334 GPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQD--VWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVIS 503
            G E  + + +C++C  SF SLQ L+ HM +TKH+   +P                  +  P  AI+      + P  K   +    PP +   +G A   ++S+             P      Q+   +  + E +   ILKCM CG S  +L  +T HM  T H+ KV +
Sbjct:  299 GKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHY-QKVP------------------LKEPVPAITK-----LVPSTKKRALQDLAPPCSPEPAGMAAEVALSESAKDQKAANPYVTPNNRYGYQNGASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKVTT 446          

BLAST of tiptop vs. SwissProt
Match: gi|85541970|sp|Q5DTH5.2|TSH1_MOUSE (RecName: Full=Teashirt homolog 1; AltName: Full=Serologically defined colon cancer antigen 3 homolog)

HSP 1 Score: 59.3066 bits (142), Expect = 2.009e-7
Identity = 45/170 (26.47%), Postives = 73/170 (42.94%), Query Frame = 0

Query:  334 GPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVIS 503
            G E  + + +C++C  SF SLQ L+ HM +TKH+       P         P  + ++ S        L+SP +P            P+  +T  A + S+  ++     +    P  R   +    +  + E +   ILKCM CG S  +L  +T HM  T H+ KV +
Sbjct:  299 GKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPV--------PAITKLVPSTKKRALQDLASPCSP-----------EPTGVATEVALSESAKDQKTANPYVT---PNNRYGYQNGASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKVTT 446          

BLAST of tiptop vs. SwissProt
Match: gi|308191590|sp|B3DJM5.1|TSH1_DANRE (RecName: Full=Teashirt homolog 1; AltName: Full=Teashirt-like zinc finger protein)

HSP 1 Score: 58.5362 bits (140), Expect = 3.456e-7
Identity = 51/176 (28.98%), Postives = 76/176 (43.18%), Query Frame = 0

Query:  334 GPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMM-----MTSPTT--AISSHLSSPMAPVVKTTHMMASTP-PSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKV 501
            G E  + + +C++C  SF SLQ L+ HM +TKH+   +P     P   S   P++       + SP +  +ISS    P+A    T     S P  +A++  G  N +S +                        W  + E +   ILKCM CG S  +L  +T HM  T H+ KV
Sbjct:  341 GKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHY-QKVPLKEPMPALASKLVPSTKKRVFQDLMSPCSPDSISSTPGIPLAETAPTKDPKISNPYVTANNRYGYQNGASYT------------------------W--QFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKV 489          

BLAST of tiptop vs. SwissProt
Match: gi|85541971|sp|Q63HK5.2|TSH3_HUMAN (RecName: Full=Teashirt homolog 3; AltName: Full=Zinc finger protein 537)

HSP 1 Score: 57.3806 bits (137), Expect = 8.399e-7
Identity = 43/173 (24.86%), Postives = 72/173 (41.62%), Query Frame = 0

Query:  334 GPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQ-----LPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKV 501
            G E  + + +C++C  SF SLQ L+ HM +TKH+       P +P                       +++ + P  +    +    PS+  ++G    +++S     D L++       P  R   +    +    E +   ILKCM CG S  +L  +T HM  T H+ KV
Sbjct:  267 GKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPVTP-----------------------VAAKIIPATRKKASLELELPSSPDSTGGTPKATISDTN--DALQKNSNPYITPNNRYGHQNGASYAWHFEARKSQILKCMECGSSHDTLQELTAHMMVTGHFIKV 414          

BLAST of tiptop vs. SwissProt
Match: gi|85541972|sp|Q8CGV9.2|TSH3_MOUSE (RecName: Full=Teashirt homolog 3; AltName: Full=Zinc finger protein 537)

HSP 1 Score: 56.9954 bits (136), Expect = 9.783e-7
Identity = 47/170 (27.65%), Postives = 66/170 (38.82%), Query Frame = 0

Query:  334 GPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGA--ANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKV 501
            G E  + + +C++C  SF SLQ L+ HM +TKH+       P +P    + P A                       K   +    P S  ST G   A  S  S    ++      P  R   +    +    E +   ILKCM CG S  +L  +T HM  T H+ KV
Sbjct:  267 GKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPVTPVAAKIIPAAR----------------------KKPSLELELPSSPDSTGGTPKATLSDASDALQKNSNPYITPNNRYGHQNGASYAWHFEARKSQILKCMECGSSHDTLQELTAHMMVTGHFIKV 414          

BLAST of tiptop vs. nr
Match: gi|929354011|ref|XP_014086099.1| (PREDICTED: protein tiptop [Bactrocera oleae])

HSP 1 Score: 350.517 bits (898), Expect = 6.329e-98
Identity = 268/774 (34.63%), Postives = 384/774 (49.61%), Query Frame = 0

Query:  135 LLSLNPSLYAVQLAQLQAAQLLSQSVGLHKSNPDLAASIAEEE-----FNMRKRKLAMQDEEAEMGAGVLNLSKRSPLGSPK-----MRAESPLDLSGNGGGGRRPSDLMSP-----AKAPKLDIPPWATGAF-PFLNPFLAVSSSGMVMSPGG-------VGPMGLPPTSSA-----MSSPPPSSSVTSSENRNPLERMSEIAKG---SANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTS---------------------------------------------SQNHVN--------AVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRG-GGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNED----------TPGKISCEKCGEKILMHIFVDHIRQCV-GPLHIPRSSP------------SMERLHRQTPSTSPPVSTPDPK--APSPVSHKSSSDHQPLGPVKEENK--------SILGSLEKMVQSNFGTK-------TKAGPDSNM------SILQRLGIDEGVDYSKPLVDPMAM 777
            LL  NP   A  L Q QA Q  S +     + P    + A  +     F+ ++R+++ ++ ++E      N  + +  G  +     M   SPLDL+   GG +R + +  P      K+ +  +PP A   + P +NP+LA  ++  +  P          G   L P  +      M+      S T S    P  ++S  A G   SA+NA  G R SAWQS W+++G +A +D+F+CVWCK SFP+L SLT HMKET+H G ++P              A+   T+     + + S+     ++ T    +T  + ++ + A   ++ +K     ++KE +PLPRKLVRGQDVWLG+G +QT++ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D + S                                             S N+ N        AVLTCKVCDQA++TLKELS+HM+KN+HYKE                       MR  GG     M      GN +        GG   +EKRKKSLPVRKLLELERAQQ+    +K+ + D             KI+CEKCGEKI   +FVDHIRQC+ G L +P  +P            +ME+L   +P T  P S  D +  A S +SH S++  + L P+ +E +        S+L ++E++++ +F T+       T +G  S        SIL+RLGID+ VDY+KPL+DP  M
Sbjct:  110 LLLTNPLAAAATLVQ-QATQAHSPTTNRQPTTPTSPQTDATNDEPVLDFSTKRRRVSERESDSEDD----NDERHADYGGIQIAETAMDVNSPLDLTV--GGRKRAATVEEPPHIAMRKSLRTALPPAAASNWVPTINPYLAAVAAASLSPPKSPLSPLEWSGKHKLLPNEATKALEKMTEMTRLGSATDSAELPPHRQLS--ANGNNSSASNAGNGGRHSAWQSHWLNKGADAVRDVFKCVWCKQSFPTLASLTVHMKETQHCGVNMP--------------ATASATTTGIPTNGNQSAQSTHHLRHTANAGTTSAATTTATTAHTTTAANKSDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTREILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSTDDKNSNGSSATSNGGGGGGTTTTSTSPVVTTPPASVGGQNTTAAMLQSSPSHNNTNNSASATVSAVLTCKVCDQAFATLKELSNHMMKNSHYKEHI---------------------MRAMGGVEHAAMTPTTTAGNTNAGR----RGGRQLREKRKKSLPVRKLLELERAQQD----YKLNSLDPALKPLRDFAATTKITCEKCGEKIETALFVDHIRQCLGGMLLVPARNPNSISDSLKSTFNAMEQL--ASPQT--PTSRSDGRKTADSNMSHASATRPEILCPLPKEGEKTSTSSTPSVLNAIEQLIEKSFDTRPRNSSNYTGSGNGSQRSTPLGSSILKRLGIDDSVDYTKPLLDPQTM 827          

HSP 2 Score: 98.9821 bits (245), Expect = 4.102e-17
Identity = 65/159 (40.88%), Postives = 82/159 (51.57%), Query Frame = 0

Query: 1100 PLAALQLLCDKTEKTPHTPVTSTTMYGVPTD---PGAILAFSWACNQAVVN---DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKA----GGGPGSAGPGNPHDKGGSPGGQNQGVEGQMKE-------ENAQSKFQKYSQLAKQLS 1241
            PLAALQ LC+ TEK P     S T  G+  +    G ++AFSWACN AV N   +S++KC  CD  F SKGAYRHH SK+H+ K+    +      K+       P SAG           G       G M         E++QSKF KY++LAKQLS
Sbjct: 1058 PLAALQKLCETTEK-PSMKTRSATTNGISHNSQLTGNMMAFSWACNDAVQNTTTESMIKCAQCDAVFSSKGAYRHHFSKVHYSKEDGELNALANAIKSPSSLACSPKSAGVSPKSSSKSPVGA------GSMSAPIINPYGESSQSKFLKYTELAKQLS 1209          

BLAST of tiptop vs. nr
Match: gi|1041532856|ref|XP_017305076.1| (PREDICTED: LOW QUALITY PROTEIN: protein tiptop-like [Diaphorina citri])

HSP 1 Score: 351.673 bits (901), Expect = 7.016e-98
Identity = 244/701 (34.81%), Postives = 335/701 (47.79%), Query Frame = 0

Query:  185 LAMQDEEAEMGAGVLNLSKRSPLGSP-KMRAESPLDLSGNGGGGRRPSDLMSPA-----KAPKLD-------IPPWATGAFPFLNPFLAVSSSGMVMSPGGVGPMGL--PPTSSAMSSPPPSSSVTSSENRNPLERMSEIAKGSANNAK---GGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTSSQ---------------------------------------------NHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKM--------GNEDT--------PGKISCEKCGEKILMHIFVDHIRQCVG-------------------PLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAGPDSNM----------SILQRLGIDEGVDYSKPLVDPMAM 777
            LAM  +  +    VLNLSK  PL +P +    SPLDLS +    +R  + +SP+     K+ ++        + PW +   P   PF A +++   +SP     +    P  +  M  P  +S  +SS+    LE+MSE++K   +  +    G R SAWQS W+++G +  KD+ +CVWCK SFPSL +LTTHMKE KH G +IPP          + P + + TSP+                                     ++ SK     ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQ+T+HYT +ISQEQ++SWKSAD   ++                                              +HV+AVLTCKVCDQA+++LKELS+HMVKN+HYKE                +        GG R                            +EKRKKSLPVRKLLE+ERAQ EM    K         GN +T         G+I+CEKCGEKI M +FVDHIR C+G                        R S  ++R   QTP+        D   P+ +S  S  D +   P      S+L ++EK+++ +F ++++ G  +            SIL+RLGIDE VDY+KPLVD   M
Sbjct:  289 LAMNMKRPDSSEPVLNLSKPIPLSTPNESLVNSPLDLSCSTR--KRSREELSPSPTSMKKSSRISNDFKPQIVTPWTSPGVPNHFPFFAAAAAAAGLSPTNTNLLATDNPLWNGKMKVPEKNSFNSSSDATKALEKMSELSKLGGDEFRPHNAGGRHSAWQSHWLNKGADQAKDVLKCVWCKQSFPSLAALTTHMKEAKHCGVNIPPPAQPNPLPPPQVPTTTVNTSPS-------------------------------------NTNSKADLNLLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQQTQHYTNIISQEQIISWKSADGNNTANKDSGDTKSTTGTNNTTNTNTPTTHSTSTGSSGSGSGSGGGTPVASSHVSAVLTCKVCDQAFTSLKELSNHMVKNSHYKE----------------HIMRSITESGGRR-------------------------RQTREKRKKSLPVRKLLEIERAQNEMRKSSKXNEEWGHVHGNGETRTRNAMHSVGRITCEKCGEKIDMAVFVDHIRLCIGGDQRNLLKSALLSSENTTSVTQALRESRKIKREDSQTPT--------DGNNPADLSTNSQKDGKSSSP------SVLNAIEKLIEKSFDSRSRHGNYAGQGGVGNAPIGSSILKRLGIDESVDYTKPLVDAQTM 895          

HSP 2 Score: 133.265 bits (334), Expect = 1.386e-27
Identity = 84/172 (48.84%), Postives = 94/172 (54.65%), Query Frame = 0

Query: 1096 KKSHPLAALQLLCDKTEKTPHTPVT-------------------------STTMYGVPTDPGAILAFSWACNQAVVN-DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQMKEENAQSKFQKYSQLAKQLS 1241
            K SHPLAALQ LCDKTE   H P +                         +TT  G  T PGAILAFSWACN AVV  DS++KC FCDTPFISKGAYRHHLSKMHFVKDG  PD     ++      S+          S G        Q  EE+  SKF KY++LAKQLS
Sbjct: 1098 KSSHPLAALQKLCDKTET--HHPTSNNSNTRPNSNSGPSVPSNSVSPSNLTTTSSG--TSPGAILAFSWACNDAVVTADSIMKCAFCDTPFISKGAYRHHLSKMHFVKDGVIPDPVTVKSQTNSPSPSSSKSMNPPNSSSSGKS----PPQNFEESPHSKFLKYTELAKQLS 1261          

BLAST of tiptop vs. nr
Match: gi|242003000|ref|XP_002422573.1| (tiptop, putative [Pediculus humanus corporis] >gi|212505363|gb|EEB09835.1| tiptop, putative [Pediculus humanus corporis])

HSP 1 Score: 349.747 bits (896), Expect = 7.190e-98
Identity = 248/687 (36.10%), Postives = 346/687 (50.36%), Query Frame = 0

Query:  182 KRKLAMQDEEAEMGAGVLNLSKRSPLGSPKMRAE--SPLDLSGNGGGGRRPSD----------LMSPAKAPKLD------IPPWATGA----FPFLNPF-------LAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAK------------GSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQ----------------TSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSG-----QFKMGNEDTPG-KISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTP--------------DPKAPS-------PVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAGPDSN-----------MSILQRLGIDEGVDYSKPLVD 773
            KR    Q E++E  A +LNLSK S   +P + AE  SPLDLS   GG +R  D               K+ ++       + PW + A    FP+           L   +S    SP   G + LP  S +  +        ++++   LE+++E++K            G+ N +    R SAWQS W+++G E  KD+ +CVWCK SFPSL ++TTHMKE KH G ++P SP                       S   +SP+ P V        T PS+S+    +      KQ    ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQ+T+HYT +ISQEQ++SWKSAD +                + + +HV+AVLTCKVCDQA+S+LKELS+HMVKN+HYKE                +     +  GG R                            +EKRKKSLPVRKLLELERAQ E+         K+  +   G +I+CEKCGEKI   IFV+HIRQC+G   +  +  ++ +    + +T P    P                ++PS       PVS K S    P G    ++ S+L ++EK+++ +F ++++ G + +            SIL+RLGIDE VDY+KPLVD
Sbjct:  215 KRSNTKQTEDSEQEA-ILNLSKAS---NPSVAAEGNSPLDLSC--GGRKRNYDDSATSSPSISPQPSRKSSRVSNDFKPVVTPWTSPALASHFPYFAAAVAAATQNLPPKNSNAENSPLWNGKLKLPEKSGSHGN--------TTDSSKALEKLNELSKLGGEDLFRSSLMGNNNISGSAGRHSAWQSHWLNKGAEQAKDVLKCVWCKQSFPSLAAMTTHMKEAKHCGVNVPSSPLQ---------------------SQAATSPIQPTV--------TSPSSSNGPTPSG-----KQDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQQTQHYTNIISQEQIISWKSADDKQGGGNTGGGPPGPGAASGASSHVSAVLTCKVCDQAFSSLKELSNHMVKNSHYKE----------------HIMRSISESGGRR-------------------------RQTREKRKKSLPVRKLLELERAQNELKNGEANLNSKISRDFVSGARITCEKCGEKIETSIFVEHIRQCIGAGLVSNNQKNLLKNALLSEATMPTNFLPREERKKGDDSGSYKQHRSPSATDLSSTPVSPKESG---PEGKQVGDSPSVLNAIEKLIEKSFDSRSRHGANFSGGAGSGNAPIGSSILKRLGIDESVDYTKPLVD 809          

HSP 2 Score: 139.428 bits (350), Expect = 1.523e-29
Identity = 78/161 (48.45%), Postives = 92/161 (57.14%), Query Frame = 0

Query: 1094 KKKKSHPLAALQLLCDKTEKTPHTPVTS------------TTMYGVPTDPGAILAFSWACNQAVV-NDSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQMKEENAQSKFQKYSQLAKQLS 1241
            K   SHPLAALQ LCDKTE   H+  ++              +    T PGAILAFSWACN AVV +DS++KC FCDT FISKGAYRHHLSKMHFVKDG  PD      +   G G+            P  + +       EE+  SKF KY++LAKQLS
Sbjct: 1018 KTTSSHPLAALQKLCDKTET--HSTASNKTNSGSTSTSGGGNVQAPSTAPGAILAFSWACNDAVVTSDSIMKCAFCDTQFISKGAYRHHLSKMHFVKDGVIPDAVALKVQTTSGKGAPTSRTASSPNSVPSSRAKSPPQPTFEESPHSKFLKYTELAKQLS 1176          

BLAST of tiptop vs. nr
Match: gi|906458302|gb|KNC21537.1| (Protein tiptop [Lucilia cuprina])

HSP 1 Score: 344.354 bits (882), Expect = 7.455e-96
Identity = 247/675 (36.59%), Postives = 325/675 (48.15%), Query Frame = 0

Query:  216 SPLDLSGNGGGGRRPSDLMSP--------AKAPKLDIPPWATGAFPFLNPFLAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAK--GSANN---------------------AKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTSSQNH------------------------------------VNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNG-TNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNED----------TPGKISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQ---------------------PLGPVKEEN----KSILGSLEKMVQSNF----------GTKTKAGPDSNMSILQRLGIDEGVDYSKPLVDPMAM 777
            SPLDLS N G  +R SD   P        A++      P ++   P +NP+LA  ++   MSP    P+ L   +    +P        +E    LE+M+E+ +  GSA                            R SAWQS W+++G +A KD+F+CVWCK SF +L SLTTHMKET+H G ++PPS      V     A+    + TT  S H          T    A+   + SS +        SK     ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D +TS  N                                     V+AVLTCKVCDQA+STLKELS+HMVKNNHYKE          MI   +   N  T +RG GR                           A+EKRKKSLPVRKLLE+ERAQ +    FK    D             K++CE CG+K+   +FVDHIRQC+    +   SP  +  +  T  +    STP  +  SP +  S SD +                     PL P + E      S+L +LE++++ +F          G  +  G     SIL+RLGID+ VDY+KPL+DP  M
Sbjct:  202 SPLDLSVNHGR-KRQSDEEEPVLISNRKLARSNDYKSLPASSTWVPPINPYLAAVAAAN-MSPKT--PLSLDWNAKTKIAP--------NEATKALEKMTEMTRLGGSAKTDHTENSTRHSTSTTSTSSTTTTTSNGRHSAWQSHWLNKGADAVKDVFKCVWCKQSFATLASLTTHMKETQHCGVNVPPS------VGSTSTAANSGGNQTTQSSHHSRHSTTQSSGTASAQAAALAANSSATSGG-----SKSDLNMLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSTDEKTSRDNSTSSNRGSSTNAATNNNATVTVNNSALSPNANATNSTVSAVLTCKVCDQAFSTLKELSNHMVKNNHYKE---------HMIRTAAAAENVPTMIRGRGR--------------------------QAREKRKKSLPVRKLLEMERAQHD----FKAAGLDQALKPLRDFAVSTKLACETCGDKVETAMFVDHIRQCIDRSLV--MSPRSQNNNNNTMKSPFGPSTPQERLSSPQTPHSRSDGRKTSDGNSSPLSATNTRADILCPLPPTESEKSSGGTSVLSALEQLIEKSFDSRPRQSSAYGEVSSGGTPLGSSILKRLGIDDNVDYTKPLMDPQTM 812          

HSP 2 Score: 102.064 bits (253), Expect = 4.901e-18
Identity = 74/196 (37.76%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 1093 SKKKKSHPLAALQLLCDKTEKTPHTPVTST--------TMYGVPT--------------------DPGAILAFSWACNQAVVN-DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMF---MAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQMK---------------EENAQSKFQKYSQLAKQLS 1241
            +KK  ++PLAALQ LC+ T+    TP  +T            +PT                       ++LAFSWACN AVV  DS++KC FCDTPF SKGAYRHHLSK+HFVKD    D        A +   P S           SP  Q Q  +                   +E+ QSKF KY++LAKQLS
Sbjct: 1014 AKKTNANPLAALQKLCETTD----TPAANTRSSSNSSHHNNNLPTHSSSSSAGTSNAGGTSASSGSSASMLAFSWACNDAVVTADSIIKCSFCDTPFSSKGAYRHHLSKVHFVKDDQHHDMNSMKSPAAMSAISPKSMTSPRSMTSAKSPAAQQQQQQRLETSPPSSVATTPTINPYDESPQSKFLKYTELAKQLS 1205          

BLAST of tiptop vs. nr
Match: gi|1098689884|ref|XP_018802689.1| (PREDICTED: protein tiptop [Bactrocera latifrons])

HSP 1 Score: 343.584 bits (880), Expect = 1.524e-95
Identity = 260/776 (33.51%), Postives = 380/776 (48.97%), Query Frame = 0

Query:  135 LLSLNPSLYAVQLAQLQAAQLLSQSVGLHKS---NPDLAASIAE--EEFNMRKRKLA-----MQDEEAEMGA---GVLNLSKRSPLGSPKMRAESPLDLSGNGGGGRRPSDLMSP------AKAPKLDIPPWATGAF-PFLNPFLAVSSSGMVMSPGG-------VGPMGLPPTSSA-----MSSPPPSSSVTSSENRNPLERMSEIAKGS--ANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQT-------------------------------------------SSQNH--------VNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGP------GGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNED----------TPGKISCEKCGEKILMHIFVDHIRQCVGPL---------HIPRSSPSMERLHRQTPSTSPPVSTPDPK--APSPVSHKSSSDHQPLGPVKEENK--------SILGSLEKMVQSNFGTKTKAGPDS-------------NMSILQRLGIDEGVDYSKPLVDPMAM 777
            LL  NP   A  L Q QA Q  S + G   +   +P + A+  E   +F+ ++R+++      +D+  E  A   G+ N           M   SPLDL+   GG +R + +  P       K+ +  +P  A   + P +NP+LA  ++  +  P          G   L P  +      M+      S T S    P  ++S     S  A+N   G R SAWQS W+++G +A +D+F+CVWCK SFP+L +LT HMKET+H G ++P + ++P   +   P +   ++ +T    H ++             ++  + ++ +G    ++ +K     ++KE +PLPRKLVRGQDVWLG+G +QT++ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D ++                                           SS +H        V+AVLTCKVCDQA++TLKELS+HM+KN+HYKE                       MR  G    G+   A+      P    GP      GG   +EKRKKSLPVRKLLELERAQQ+    +K+ + D             KI+CEKCGEKI   +FVDHIRQC+G +         ++  S  S      Q  S   P S  D +  A S +SH S++    L P+ +E +        S+L ++E++++ +F T+ +   +                SIL+RLGID+ VDY+KPL+DP  M
Sbjct:  110 LLLTNPLAAAATLVQ-QATQAHSPAAGRQPTTPTSPQMDATNDEPVLDFSTKRRRMSERESDSEDDNHERNADDGGIQN-------AEAAMDVNSPLDLTV--GGRKRAATIDEPPPHIAMHKSLRTALPTAAASNWVPTINPYLAAVAAASLSPPKSPLSPLEWSGKHKLLPNEATKALEKMTEMTRLGSATDSAELPPHRQLSANGNNSSAASNVGNGGRHSAWQSHWLNKGADAVRDVFKCVWCKQSFPTLATLTVHMKETQHCGVNMPATASAP---ATGIPTNGNQSAQSTHHLRHTTN----------TGTTSAGTTTAATGGNTTTAANKSDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTREILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSTDDKSSNSSSAASIGGSGGGTTTTSTSPVVTTPPATASGLNTTVTMRQSSPSHNNNSASATVSAVLTCKVCDQAFATLKELSNHMMKNSHYKEHM---------------------MRAMG----GVEQAAMT-----PTTTAGPTNVGRRGGRQLREKRKKSLPVRKLLELERAQQD----YKLNSLDPALKPLRDFAAITKITCEKCGEKIETALFVDHIRQCLGGMLLVPARNHNNVSDSLKSTFNAMEQLASPQTPTSRSDGRKTADSNMSHASATRPDILCPLTKEGEKASTSSTPSVLNAIEQLIEKSFDTRPRNSSNYAGSGNGSQRSTPLGSSILKRLGIDDSVDYTKPLLDPQTM 828          

HSP 2 Score: 100.138 bits (248), Expect = 2.096e-17
Identity = 65/166 (39.16%), Postives = 88/166 (53.01%), Query Frame = 0

Query: 1094 KKKKSHPLAALQLLCDKTEKTPHTPVTSTTMYGV----PTDPGAILAFSWACNQAVVN---DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKA----GGGPGSAGPGNPHDKGGSPGGQNQGVEGQMK-------EENAQSKFQKYSQLAKQLS 1241
            KK  ++PLAALQ LC+ TEK P     S T  G+        G ++AFSWACN AV N   +S++KC  CD  F +KGAYRHH SK+H+ K+ +  +  +  +K+       P SAG           G       G M         +++QSKF KY++LAKQLS
Sbjct: 1049 KKSSANPLAALQKLCETTEK-PSMKTRSATTNGIGHHQSQLTGNMMAFSWACNDAVQNTTAESMIKCTQCDAVFSTKGAYRHHFSKVHYSKEDNELNALVSASKSPSSLACSPKSAGASPKSSSKSPVGA------GSMSAPIINPFSDSSQSKFLKYTELAKQLS 1207          

BLAST of tiptop vs. nr
Match: gi|1132387982|ref|XP_019846665.1| (PREDICTED: protein tiptop [Bactrocera dorsalis])

HSP 1 Score: 343.969 bits (881), Expect = 1.545e-95
Identity = 262/783 (33.46%), Postives = 374/783 (47.77%), Query Frame = 0

Query:  135 LLSLNPSLYAVQLAQLQAAQLLSQSVGLHKSNPDLAASIAEEE-----FNMRKRKLAMQ-----DEEAEMGA---GVLNLSKRSPLGSPKMRAESPLDLSGNGGGGRRPSDLMSP------AKAPKLDIPPWATGAF-PFLNPFLAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAK-----------------------GSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDI---LKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTS---------------------------------------------SQNH------VNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRG-GGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNED----------TPGKISCEKCGEKILMHIFVDHIRQCVGPL---------HIPRSSPSMERLHRQTPSTSPPVSTPDPK--APSPVSHKSSSDHQPLGPVKEENK--------SILGSLEKMVQSNFGTKTKAGPDS-------------NMSILQRLGIDEGVDYSKPLVDPMAM 777
            LL  NP   A  L Q QA Q  S +     + P    + A  +     F+ ++R+++ +     D+  E  A   G+ N           M   SPLDL+   GG +R + +  P       K  +  +PP A   + P +NP+LA  ++  +  P    P+  P   S      P+      E    LE+M+E+ +                        +A+N   G R SAWQS W+++G +A +D+F+CVWCK SFP+L +LT HMKET+H G ++P +  +P                TT I ++ +          H   +   SA++T+ A   ++ +     D+   +KE +PLPRKLVRGQDVWLG+G +QT++ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D ++S                                             S N+      V+AVLTCKVCDQA++TLKELS+HM+KN+HYKE                       MR  GG     M      G   P + G   GG   +EKRKKSLPVRKLLELERAQQ+    +K+ + D             KI+CEKCGEKI   +FVDHIRQC+G +          +  S  S      Q  S   P S  D +  A S +SH S++  + L P+ +E +        S+L ++E++++ +F T+ +   +                SIL+RLGID+ VDY+KPL+DP  M
Sbjct:  137 LLLTNPLAAAATLVQ-QATQAHSPTTNRQPTTPTSPQTDANNDEPVLDFSTKRRRISERESDSDDDNHERSADDGGIQN-------ADAAMDVNSPLDLTV--GGRKRAATIDEPPPHLAMRKNLRTALPPAAASNWVPTINPYLAAVAAASLSPPKS--PLS-PLEWSGKHKLLPN------EATKALEKMTEMTRLGSATDSAELPSHRQLSANGNNSSAASNVGNGGRHSAWQSHWLNKGADAVRDVFKCVWCKQSFPTLATLTVHMKETQHCGVNMPATAATP----------------TTGIPTNGNQSAQSAHHLRHTANAGTTSAATTTAATGGNTTTAANKSDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTREILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSTDDKSSNGSSAASIGGGGGGTTTTSTSPVVTTPPATASGLNTTVAMRQSSPSHNNNSTSATVSAVLTCKVCDQAFATLKELSNHMMKNSHYKEHM---------------------MRAMGGVEHATMTPTTTAG---PTNAGRR-GGRQLREKRKKSLPVRKLLELERAQQD----YKLNSLDPALKPLRDFAATTKITCEKCGEKIETALFVDHIRQCLGGMLLVPARNHNSVSDSLKSTFNAMEQLASPQTPTSRSDGRKTADSNMSHASATRPEILCPLPKEGEKTSTSSTPSVLNAIEQLIEKSFDTRPRNSSNYAGSGNGSQRSTPLGSSILKRLGIDDSVDYTKPLLDPQTM 855          

HSP 2 Score: 93.9745 bits (232), Expect = 1.528e-15
Identity = 61/160 (38.12%), Postives = 81/160 (50.62%), Query Frame = 0

Query: 1100 PLAALQLLCDKTEKTPHTPVTSTTMYGVPTD----PGAILAFSWACNQAVVN---DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKA----GGGPGSAGPGNPHDKGGSPGGQNQGVEGQMK-------EENAQSKFQKYSQLAKQLS 1241
            PLAALQ LC+ TEK P     S    G+        G ++AFSWACN AV N   +S++KC  CD  F +KGAYRHH SK+H+ K+    +  +  +K+       P S G           G       G M         +++QSKF KY++LAKQLS
Sbjct: 1082 PLAALQKLCETTEK-PSMKTRSANTNGISNHQSQLTGNMMAFSWACNDAVQNTTAESMIKCTQCDAVFSTKGAYRHHFSKVHYSKEDGELNALVSASKSPSSLACSPKSTGASPKSSSKSPVGA------GSMSAPIINPFSDSSQSKFLKYTELAKQLS 1234          

BLAST of tiptop vs. nr
Match: gi|1321319121|ref|XP_023301926.1| (protein tiptop [Lucilia cuprina])

HSP 1 Score: 340.117 bits (871), Expect = 1.320e-94
Identity = 236/650 (36.31%), Postives = 313/650 (48.15%), Query Frame = 0

Query:  216 SPLDLSGNGGGGRRPSDLMSP--------AKAPKLDIPPWATGAFPFLNPFLAVSSSGMVMSPGGVGPMGLPPTSSAMSSPPPSSSVTSSENRNPLERMSEIAK--GSANN---------------------AKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSADSQTSSQNH------------------------------------VNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNG-TNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNED----------TPGKISCEKCGEKILMHIFVDHIRQCVGPLHIPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNF----------GTKTKAGPDSNMSILQRLGIDEGVDYSKPLVDPMAM 777
            SPLDLS N G  +R SD   P        A++      P ++   P +NP+LA  ++   MSP    P+ L   +    +P        +E    LE+M+E+ +  GSA                            R SAWQS W+++G +A KD+F+CVWCK SF +L SLTTHMKET+H G ++PPS      V     A+    + TT  S H          T    A+   + SS +        SK     ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQET+HYT +ISQEQ++SWKS D +TS  N                                     V+AVLTCKVCDQA+STLKELS+HMVKNNHYKE          MI   +   N  T +RG GR                           A+EKRKKSLPVRKLLE+ERAQ +    FK    D             K++CE CG+K+   +FVDHIRQC+    +                           +P   ++ +++   P GP      S+L +LE++++ +F          G  +  G     SIL+RLGID+ VDY+KPL+DP  M
Sbjct:  202 SPLDLSVNHGR-KRQSDEEEPVLISNRKLARSNDYKSLPASSTWVPPINPYLAAVAAAN-MSPKT--PLSLDWNAKTKIAP--------NEATKALEKMTEMTRLGGSAKTDHTENSTRHSTSTTSTSSTTTTTSNGRHSAWQSHWLNKGADAVKDVFKCVWCKQSFATLASLTTHMKETQHCGVNVPPS------VGSTSTAANSGGNQTTQSSHHSRHSTTQSSGTASAQAAALAANSSATSGG-----SKSDLNMLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQETQHYTNIISQEQIISWKSTDEKTSRDNSTSSNRGSSTNAATNNNATVTVNNSALSPNANATNSTVSAVLTCKVCDQAFSTLKELSNHMVKNNHYKE---------HMIRTAAAAENVPTMIRGRGR--------------------------QAREKRKKSLPVRKLLEMERAQHD----FKAAGLDQALKPLRDFAVSTKLACETCGDKVETAMFVDHIRQCIDRSLV--------------------------MSPRSQNNNNNTMKSPFGPSTP--PSVLSALEQLIEKSFDSRPRQSSAYGEVSSGGTPLGSSILKRLGIDDNVDYTKPLMDPQTM 761          

HSP 2 Score: 102.064 bits (253), Expect = 4.859e-18
Identity = 74/196 (37.76%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 1093 SKKKKSHPLAALQLLCDKTEKTPHTPVTST--------TMYGVPT--------------------DPGAILAFSWACNQAVVN-DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMF---MAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQMK---------------EENAQSKFQKYSQLAKQLS 1241
            +KK  ++PLAALQ LC+ T+    TP  +T            +PT                       ++LAFSWACN AVV  DS++KC FCDTPF SKGAYRHHLSK+HFVKD    D        A +   P S           SP  Q Q  +                   +E+ QSKF KY++LAKQLS
Sbjct:  963 AKKTNANPLAALQKLCETTD----TPAANTRSSSNSSHHNNNLPTHSSSSSAGTSNAGGTSASSGSSASMLAFSWACNDAVVTADSIIKCSFCDTPFSSKGAYRHHLSKVHFVKDDQHHDMNSMKSPAAMSAISPKSMTSPRSMTSAKSPAAQQQQQQRLETSPPSSVATTPTINPYDESPQSKFLKYTELAKQLS 1154          

BLAST of tiptop vs. nr
Match: gi|1009369669|gb|KYM94255.1| (Protein tiptop, partial [Cyphomyrmex costatus])

HSP 1 Score: 338.191 bits (866), Expect = 1.338e-94
Identity = 211/547 (38.57%), Postives = 279/547 (51.01%), Query Frame = 0

Query:  297 SENRNPLERMSEIAK------------------GSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSS---VSKQQYRDI---LKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSAD--------------------SQTSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEMSGQFKMGNEDT---------PGKISCEKCGEKILMHIFVDHIRQCVGPLHI---PRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAG--------PDSNM--SILQRLGIDEGVDYSKPLVDPMAM 777
            SE    LE+MSE++K                  G+A       R SAWQS W+++G +  KD+ +CVWCK SFP+L ++TTHMKE KH G ++P          V PPA  + +  TT             + T           +ST    +NSS    SKQ   D+   +KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQ+T+HYT +ISQEQ++SWKS+D                       ++ +HV+AVLTCKVCDQA+S+LKELS+HMVKN+HYKE                +        GG R                            +EKRKKSLPVRKLLELERAQ E    FK G+             G+I+CEKCGEKI   +FV+HIRQC+G   +    R+      +    P+T PP  +                          + S+L ++EK+++ +F ++ + G        P + M  SIL+RLGIDE VDY+KPLVDP  M
Sbjct:  318 SEATKALEKMSELSKLGGEDLFRSSSGSGAATGGNAPATTSSSRHSAWQSHWLNKGADQAKDVLKCVWCKQSFPTLAAMTTHMKEAKHCGVNMP----------VPPPAPGIHSQQTT-------------MPTIPPPQQPHQPQTSTGNPGSNSSGTPSSKQSPSDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQQTQHYTNIISQEQIISWKSSDESKGGNATGAGGGSGNVSGGGGGATSSHVSAVLTCKVCDQAFSSLKELSNHMVKNSHYKE----------------HIMRSITESGGRR-------------------------RQTREKRKKSLPVRKLLELERAQNE----FKNGDAAAGLTHSLGKHAGRITCEKCGEKIETPLFVEHIRQCIGAGTVGANQRNFLKNALMSNHLPATLPPSES-----------------------GRTSPSVLNAIEKLIEKSFDSRVRHGTPGLHHAQPGAPMGTSILKRLGIDESVDYTKPLVDPQTM 773          

HSP 2 Score: 128.642 bits (322), Expect = 3.203e-26
Identity = 86/184 (46.74%), Postives = 96/184 (52.17%), Query Frame = 0

Query: 1087 ANEQGRSKKKKSHPLAALQLLCDKTE------KTPHTPVTSTTMYGVPTDPG-AILAFSWACNQAVVN-DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPD---------------HKMFMAKAGGGP--GSAGPGNPHDKGGSPGGQNQGVEGQMK----EENAQSKFQKYSQLAKQLS 1241
             + QGR  K  SHPLAALQ LCDKTE              S+    + +  G AILAFSWACN AVV  DS++KC FCDTPFISKGAYRHHLSKMHFVKDG  PD                        GGP   S+ P         P  Q  G   Q      EE+  SKF KY++LAKQLS
Sbjct:  881 VDSQGR--KTSSHPLAALQKLCDKTETRGPGASGTSRSGGSSAASTIGSGSGSAILAFSWACNDAVVTADSIMKCAFCDTPFISKGAYRHHLSKMHFVKDGVIPDPLTIGRSAAAAAAAAAAAVSQGTSGGPSRNSSSPPTSQRNKSPPLPQANGSPSQSAASPLEESPHSKFLKYTELAKQLS 1062          

BLAST of tiptop vs. nr
Match: gi|1080043379|ref|XP_018562792.1| (protein tiptop [Anoplophora glabripennis])

HSP 1 Score: 339.347 bits (869), Expect = 1.425e-94
Identity = 215/548 (39.23%), Postives = 284/548 (51.82%), Query Frame = 0

Query:  295 TSSENRNPLERMSEIAK--------GSANNAKGGP---RQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDILKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSAD---------------SQTSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEM----SGQF--KMGNEDTPGKISCEKCGEKILMHIFVDHIRQCVGPLH----------------IPRSSPSMERLHRQTPSTSPPVSTPDPKAPSPVSHKSS----SDHQPLGPVKEEN---KSILGSLEKMVQSNFGTKTKAGPD----------SNMSILQRLGIDEGVDYSKPLVDPMAM 777
            T S+    LE+MSE++K          AN A G     R SAWQS W+++G E  KD+ +CVWCK SFPSL ++TTHMKE KH G ++P  P             M  TS            + P  + +    +   ++S+ S   N S ++      ++KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQ+T+HYT +ISQEQ++SW+S D                   + +HV+AVLTCKVCDQA+S+LKELS+HMVKN+HYKE                +        GG R                            +EKRKKSLPVRKLLELERAQ E     +G    KM +    G+I+CEKCG KI   +FVDHIRQCVG +                 +P  SPS ++L            TP  K+PSP     S    S  +P    +  N    S+L ++EK+++ +F ++++  P              SIL+RLGIDE VDY+KPLVD   M
Sbjct:  287 TPSDATKALEKMSELSKLGGEDLFRSMANAAPGNSASNRHSAWQSHWLNKGAEQAKDVLKCVWCKQSFPSLAAMTTHMKEAKHCGVNVPVPP-------------MQSTS------------LIPQPQISSPSNTNTSTSSTNSNKPNQSDLNM-----LIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQQTQHYTNIISQEQIISWRSTDDAKGGGGGGPSGPNPPPGGASSHVSAVLTCKVCDQAFSSLKELSNHMVKNSHYKE----------------HIMRSITESGGRR-------------------------RQTREKRKKSLPVRKLLELERAQHEFKNGDNGSIIEKMRDPTGAGRITCEKCGNKIETSLFVDHIRQCVGGMTNNQRNFLKNALMSSNILPPESPSRDKL-----------KTPTDKSPSPQQRSPSVSDLSTKEPSATPENNNGSSPSVLNAIEKLIEKSFDSRSRQNPGFPGQGPTQAPMGSSILKRLGIDESVDYTKPLVDAQTM 752          

HSP 2 Score: 130.183 bits (326), Expect = 1.306e-26
Identity = 86/177 (48.59%), Postives = 98/177 (55.37%), Query Frame = 0

Query: 1090 QGRSKKKKSHPLAALQLLCDKTEKTPHTPVTSTTMYGVPTDP--------------GAILAFSWACNQAVV-NDSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGSAGPGNP----------HDKGGSPGGQNQGVEGQMKEENAQSKFQKYSQLAKQLS 1241
            Q  +K+  SHPLAALQ LCDKTE   HT   S        +P              GAILAFSWACN AV+ +DS++KC FCDTPFISKGAYRHHLSKMHFVKDG  PD      K      SAG G P               S G ++    G   EE+  SKF KY++LAKQLS
Sbjct:  961 QTGNKRGSSHPLAALQKLCDKTET--HTNNRSHAAATAVANPVTASNTTTTSGTTPGAILAFSWACNDAVMTSDSIMKCAFCDTPFISKGAYRHHLSKMHFVKDGVIPDPVSL--KTSQPSSSAGSGVPLKGTAIVPTVSSSSASTGAKSPPAPGGF-EESPHSKFLKYTELAKQLS 1132          

BLAST of tiptop vs. nr
Match: gi|1009395603|gb|KYN16405.1| (Protein tiptop, partial [Trachymyrmex cornetzi])

HSP 1 Score: 338.961 bits (868), Expect = 1.805e-94
Identity = 214/567 (37.74%), Postives = 296/567 (52.20%), Query Frame = 0

Query:  297 SENRNPLERMSEIAK-------------------GSANNAKGGPRQSAWQSQWISRGPEANKDIFRCVWCKDSFPSLQSLTTHMKETKHFGASIPPSPTSPHQVSVRPPASMMMTSPTTAISSHLSSPMAPVVKTTHMMASTPPSASSTSGAANNSSVSKQQYRDI---LKEQLPLPRKLVRGQDVWLGRGEQQTKDILKCMWCGESFRSLDLMTKHMQETKHYTKVISQEQLVSWKSAD-------------------SQTSSQNHVNAVLTCKVCDQAYSTLKELSDHMVKNNHYKESQGPPTPSPGMISPGSNPTNGTNMRGGGRGSVGMPSGALLGNGSPPHPGMGPGGSSAKEKRKKSLPVRKLLELERAQQEM-SGQFKMGNEDTPGK----ISCEKCGEKILMHIFVDHIRQCVGP-----------------LHIPRSSPS---MERLHRQT----------PSTSPPVSTPDPKAPSPVSHKSSSDHQPLGPVKEENKSILGSLEKMVQSNFGTKTKAG--------PDSNM--SILQRLGIDEGVDYSKPLVDPMAM 777
            SE    LE+MSE++K                    +A       R SAWQS W+++G +  KD+ +CVWCK SFP+L ++TTHMKE KH G ++P          V PPA  + +  TT           P +           S  +T    + +  SKQ   D+   +KE +PLPRKLVRGQDVWLG+G +QT+ ILKCMWCG+SFRSL  MT HMQ+T+HYT +ISQEQ++SWKS+D                      ++ +HV+AVLTCKVCDQA+S+LKELS+HMVKN+HYKE                +        GG R                            +EKRKKSLPVRKLLELERAQ E  +G   +G   + GK    I+CEKCGEKI   +FV+HIRQC+G                   H+P   P+    + ++ +T          PS++   +TP    P+P ++++++   P+G     + S+L ++EK+++ +F ++ + G        P + M  SIL+RLGIDE VDY+KPLVDP  M
Sbjct:  334 SEATKALEKMSELSKLGGEDLFRSSSGGGGTVTGANAPATTSSSRHSAWQSHWLNKGADQAKDVLKCVWCKQSFPTLAAMTTHMKEAKHCGVNMP----------VPPPAPGIHSQQTTM----------PTIPPPQQPHQPQTSTGNTGSNTSGTPSSKQSPSDLNLLIKETMPLPRKLVRGQDVWLGKGAEQTRQILKCMWCGQSFRSLAEMTSHMQQTQHYTNIISQEQIISWKSSDDSKGGNSTGAGGGSGNVAGGAGATSSHVSAVLTCKVCDQAFSSLKELSNHMVKNSHYKE----------------HIMRSITESGGRR-------------------------RQTREKRKKSLPVRKLLELERAQNEFKNGDAAVGLTHSLGKHAGRITCEKCGEKIETPLFVEHIRQCIGAGTVGANQRNFLKNALMSNHLPALPPTESGRKSVNEETSSISSRHHRSPSSASDATTPGKDTPTPTANETTA---PMG----TSPSVLNAIEKLIEKSFDSRVRHGTPGLHHAQPGAPMGTSILKRLGIDESVDYTKPLVDPQTM 832          

HSP 2 Score: 137.502 bits (345), Expect = 6.246e-29
Identity = 82/163 (50.31%), Postives = 97/163 (59.51%), Query Frame = 0

Query: 1087 ANEQGRSKKKKSHPLAALQLLCDKTE------KTPHTPVTSTTMYGVPTDPG-AILAFSWACNQAVVN-DSLLKCPFCDTPFISKGAYRHHLSKMHFVKDGSAPDHKMFMAKAGGGPGSAGPGNPHDKGGSPGGQNQGVEGQMKEENAQSKFQKYSQLAKQLS 1241
             + QGR  K  SHPLAALQ LCDKTE       +      S+    + +  G AILAFSWACN AVV  DS++KC FCDTPFISKGAYRHHLSKMHFVKDG  PD  + + ++   P     G+P     SP            EE+  SKF KY++LAKQLS
Sbjct:  961 VDSQGR--KTSSHPLAALQKLCDKTETRGPGASSTSRSGGSSAASTIGSGSGSAILAFSWACNDAVVTADSIMKCAFCDTPFISKGAYRHHLSKMHFVKDGVIPD-PLTIGRSAAEPIPQANGSPSQSAASP-----------LEESPHSKFLKYTELAKQLS 1109