BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 739.954 bits (1909), Expect = 0.000e+0
Identity = 349/550 (63.45%), Postives = 440/550 (80.00%), Query Frame = 0

Query:   53 SAGHYTPPTIPAEDEDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIVDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            S  H+  P I   +E  I  SPY +  L     ADF+W+ +  + +  A++CGMTGR YTY+M + +S+KFGSALLRLGAKKGDVMG+VVPNIPEF IAF+G A +GVTLTTMNPTYRPEEIARQL+NS AK+++TIGLFL NI+Q+CEIY GI+KIIV+GME+ P+DC SF++M I+DDGS +DQ  + D + D++ LP+SSGTTGPPKGV+LTH N+V+N+ QL HP ++N        +     ++AVLPFFHI+AMT  ++ GL  G K++TLPKFEPE ++ +L  +KPT + +VPPLVSF++++P +K   L+S++ + GGA+ FGPTLIEKFM KC  S + F+EG+GMTE SPV+HVQP+   + G CG+ IPNT+ KIIDI+TG  LGP + GELCV+GPQ+M+GYYKNKKAT+K I +GW  TGDIA Y+ +GQF IVDRLKELIKVKGLQVAPSELEDLIR++ GV+DVA VGVPDER+GELPRAYVVRKNR+V+EQ IID+V E+ APHKKLGAGVMFVDTLPKNQTGKILRRELKAQ
Sbjct:   30 SXSHFGAPLIKPGEEKLIQYSPYEEVVLPSIPYADFIWENLEDYKEREALVCGMTGRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFI----GDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCD-SDLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 574          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 203.371 bits (516), Expect = 1.513e-57
Identity = 155/560 (27.68%), Postives = 271/560 (48.39%), Query Frame = 0

Query:   79 ELVESNLADFVWKKV-HQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHP-GVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMN--LGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQP--EKNAVLGGCGHPIPN------------TMAKIIDIETGATL-GPNQEGELCVAGPQVMKGYYKNKKATNKTIVDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRK-NRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQNP---GALVSPDRILK 615
            E+ + N  +F+ +K+  + PD I +     GR   +   + +  K  S L + G  KGDV+ +   N  E+ I  LG    G  +  +N    P+E+  Q+  S+ K ++       +     E    IEK+I++G E    D +  +  L+ DDG    Q    D   D++ +PF+S ++G  KG+  T+ +++S   Q   P G  N    +   +   ++      FFH   MT   N  LG   G  I TL +F  + +L+AL   K   +      +  LS +P V  + L+SL+ ++ G      T+  + +++  PS+   RE +G+ E   V+   P  +KN++       +P+            +M KII  + G  + GP+Q+GE+CV  PQ M GY  NK+ ++    D +  TGD+  YD+ G    V+++  LI+    +VAPS LE  +   + + D AVVG+P++ +G++PRA+VV K      E+++ + +  R   H+++  G+ FV  +P+++  K+LR  L    P     +  P+++ K
Sbjct:   55 EIQKLNFHEFILQKIGEKEPDRIWIKDLSIGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSGA-KGILHTNKSLMS---QFYSPDGAAN----HWFDQLIGDSIHCANYFFH---MTGFYNFALGSIYGISIYTLSEFSEQNFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRI-PSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYL-NKEESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAEYKPHVNNLIKRPNKLFK 601          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 129.413 bits (324), Expect = 2.372e-31
Identity = 107/383 (27.94%), Postives = 182/383 (47.52%), Query Frame = 0

Query:  246 NDLVVLPFSSGTTGPPKGVSLTHFNM---VSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEE---YLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESV--------IGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTE-SSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLG----------PNQEGELCVAGPQVMKGYYKNKKATNKTI--VDGWLLTGDIAKYDDNGQFMIVDRLK-ELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELK 600
            ND  ++ ++SGT+G PKGV +TH N+   VS M++               + TS +  + VLP +H++ +   + + L +GA++  LP F+ ++   YL   S +   F+  VP + S L+    +   +   +  +        + G+A     L+ K+       ++   E +GMTE    + +   E   + G  G P PN  A+I+  E G+ L            ++ GEL + GP V   YY N+KAT ++      W LTGD A  +  G F I+ R   ++IK  G +++  E+E     +  ++++AVVG+ DE  GE+  A +V      L  DI+     +  P   +    M V+++ KN  GKI ++ELK
Sbjct:  147 NDGALILYTSGTSGSPKGVWITHRNIEAQVSGMIKFW-------------AWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLTNSSMNISVFMG-VPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRKWQTISGHILL---ERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMS-EEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYSVPRQFMLVNSIDKNHMGKINKKELK 511          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 119.783 bits (299), Expect = 2.634e-28
Identity = 101/357 (28.29%), Postives = 165/357 (46.22%), Query Frame = 0

Query:  251 LPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPK--FEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAV---LGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIV-DGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            + F+SGTTG PK V+L+HF +++N +   +    +LK                +P FH +       + L  G+K+V LP   F+P   L  +   K T+L   P +             ++A LE+                           R  +G TE+ PVS +   K++    +   G P+ +  AKI+D + G  L  N  GEL V  P +M GYYK++K++ K I  D +  +GD+    + G   I+ RL +++   G  + P E+ED + +H  + +V +VGVPD+  GE   A++  +     E D+ +    + A H K+    MFV   PK  +GK+ + E+KA
Sbjct:    4 IQFTSGTTGLPKAVALSHFCVINNALLTGNRIGFDLKY---------HXINLWVPLFHTFGCVTGTLISLCYGSKLV-LPSDGFDPTACLNCIPAEKCTYLFGTPTM-------------YIAVLEAQ-----------------------KKLRNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLE-EGAKEFDLKEHCKGKIA-HFKIPKYPMFVKDFPKTLSGKVKKFEMKA 311          

HSP 2 Score: 98.9821 bits (245), Expect = 1.187e-21
Identity = 79/263 (30.04%), Postives = 135/263 (51.33%), Query Frame = 0

Query:  345 KPTFLHLVPPL-VSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCS-PSVVNFREGFGMTESSPVSHV-QPEKN--AVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIV-DGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            K T+L   P + V+ L     +K   ++S+E  I G +     + E F +  +  S++N    +G TE+ P + V  PE +    +   G P+ +  AKI+D + G  L  N  GEL V  P +M GYYK++K++ K I  D +  +GD+A   + G   I+ RL +++   G  + P E+ED + +H  + +V ++GVPD+  GE   A++  +     E D+ +    + A H K+    MFV   PK  +GK+ + E++ 
Sbjct:  655 KCTYLFGTPTMYVAVLEAQKKLKVD-VSSVELGITGGSNCLVEVCEDFEKHLNMKSLMNI---YGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLE-EGAKEFDLKEHCKGKIA-HFKIPKYSMFVKDFPKTLSGKVKKLEMRG 910          

HSP 3 Score: 98.9821 bits (245), Expect = 1.240e-21
Identity = 65/200 (32.50%), Postives = 108/200 (54.00%), Query Frame = 0

Query:  406 FGMTESSPVSHVQPEKNAV---LGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIV-DGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            +G TE+ PVS +   K++    +   G P+ +  AKI+D + G  L  N  GEL V  P +M GYYK++K++ K I  D +  +GD+    + G   I+ RL +++   G  + P E+ED++ +H  + +V +VGVPD   GE   A++  +     E D+ +    + + H K+    MFV   PK  +GK+ + E+KA
Sbjct:  343 YGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLE-EGAKEFDLKEHCKGKIS-HFKIPKYPMFVKDFPKTLSGKVKKFEMKA 539          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000010073 (supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10")

HSP 1 Score: 105.145 bits (261), Expect = 8.749e-24
Identity = 113/484 (23.35%), Postives = 199/484 (41.12%), Query Frame = 0

Query:  119 MSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPAD---------CLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKI-VTLPKFEPEEYLKAL-------STHKPTFLHLVPPLV-------------------SFLSTNPAVKAHH--------------LASLESVIGGAAPF----GPTLIEKFMEKCSPSV-VNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGA---TLGPNQEGELCVAGPQVMKGYYKNKKATNKTIVDG-----WLLTGDIAKYDDNGQFMIVDRLKELIKVK-GLQVAPSELEDLIRRHSGVNDVAVVGVP 538
            +S  FG  L  LG +  + + +   +  E+  + LG       L T+      + IA  +  +Q   +IT    LP ++   E  P I+ II    +    D          LSF +++  + GS  +   +    +D  ++ ++SG+TG PKGV L H +++   +   H  V +        K   ET +  LP  HIY + A M + +H G K+  + P    ++  K +       S  +PT +  VP ++                   S L      K+H+                 + +++GG        G  L     +  S ++ +   +G+G+TES     V   +    G  G P    + ++I+ E G    T  P   GE+ + GP +  GYYK  + T +          W  TGD+ ++ ++G   I+DR K+L+K++ G  V+  ++E +I+    V +V   G P
Sbjct:  151 LSTDFGKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTHDLLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVI--ELGSKSETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGT-IAAFHLVVKD--------KIPEETYLGYLPLAHIYELLAEMTMLIH-GIKVGYSSPNTMIDKSTKVMKGSKGDCSVLRPTLMCAVPLVIDRIYKGIHENLSKKSKFMRSLLHFCYHYKSHYRRLGMRTPIMNALIFRPMRAIVGGKVRVIVSGGAPLSHNIHDYVSCALDIPIHQGYGLTESCAGGSVMHREELSTGRVGPPGQGVLIRLINWEDGGYRVTDKPRPRGEIVIGGPNIAHGYYKLPQKTAEDFYKDENNVRWFKTGDVGEFAEDGTLKIIDRKKDLVKLQYGEYVSLGKVETVIKTCPLVENVCAYGDP 622          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 100.523 bits (249), Expect = 1.740e-22
Identity = 118/502 (23.51%), Postives = 206/502 (41.04%), Query Frame = 0

Query:  122 KFGSALLRLGAKKGDVMGMVVPNIPEFPI---AFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASL-ESVIGGAAPFGPTL----------------IEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQV-MKGYYKNKKATNKTIVD-GWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRR-HSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELK 600
            KF   L   G +KGDV+ +++ N  +  I   A L   GV V+++  N +   E +  +++ +QAKF+I        ++    I   ++  +   +E   +      + + +D                L V+ ++SGTTG PKG+ L       NMV L      NL   +P +  +   T     F H+       +    +   + T     PE+ + +L   K  F     P      T   V A H+  + E ++     +                    I   + K   ++ N    + MTES  +  +  ++ +  G  G    N    I DI +G  LGPN  GEL    P     GY      T K  +D GW  +GD+  Y+D+G+     R+KE++K  G  V P E+E +I+  +S + +  +    ++    L    V++K   +  ++I  +V +R   +K L   ++  + LP+N  GK+LR ++ 
Sbjct:   85 KFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGV-VSISVDNIS--TETLLSEVQETQAKFVICTNRTYYKVKDLSSIIINLDTQLHAILEIDMSTQTQIPEQIEFD-------------LQTLAVILWTSGTTGKPKGIQL-------NMVSLW-----NLLFLSPKTDGNMLITCN---FAHVSGFN--YSCFWSINFDVCTFHFISPED-VTSLGVEKIIFTLSQIPF-----TTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVG----NMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKIQTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRNDIN 543          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000004934 (supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9")

HSP 1 Score: 100.523 bits (249), Expect = 2.846e-22
Identity = 114/533 (21.39%), Postives = 220/533 (41.28%), Query Frame = 0

Query:  112 TYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPG-IEKIIVLGMED---------KPADCLSFIDMLIYDDGSLYDQDRSCDVY---NDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMT---AIMNLGLHLG----AKIVTLPKFEPEEYLKALSTHKPTFLHLVP------------------------------------------PLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATL---GPNQEGELCVAGPQVMKGYYKNKKATNKTIVDG----WLLTGDIAKYDDNGQFMIVDRLKELIKVK-GLQVAPSELEDLIRRHSGVNDVAVVG-----------VPDERSGELPRAYVVRKNRNVLEQDII 563
            TY+    +SR F   L  L     D + +   +  ++ ++  G     +T+ T+      E +   +E + AK IIT    L  +++      G I+ II L  E+         +  +  SF ++L     S    D   D      D+ V+ ++SG++G PKGV L+HFN++SN+  ++     N        +   E+ +  LP  HI+ ++   A+   G+ +G      ++       E  +  +   KPT++ +VP                                          P+++ L     ++     ++ +++ G AP      E FME      +   +G+G+TE+     +  +++  LG  GHP P    K+ + + G       P   GE+ V G  +  GY++    T +   +     W  TGDI +   +G   I+DR K++IK++ G  V+  ++E  +  H  V+ + V G           +P+E+S ++ +  +  +N +V + +++
Sbjct:  215 TYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQNLLKRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAVIMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNF------VRLDKESYIGYLPLAHIFELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKWRKLGMNTPIINLLFF-KKIQQFLGGNVSTIVSGGAPLSAQAHE-FMEIVFD--LKLSQGYGLTETCGGFILGRKEDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYLGTHPLVDIICVYGEPERNCIVGLIIPNEKSIKILKESLGLRNESVYDNNLL 737          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 93.5893 bits (231), Expect = 2.227e-20
Identity = 67/222 (30.18%), Postives = 111/222 (50.00%), Query Frame = 0

Query:  383 FGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIV--DGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIR-RHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKL-GAGVMFVDTLPKNQTGKILRRELK 600
            FG   + + ++KC P   NF   +G TES     V   +++     G  +  T   I D+ TG  LGPN+ GEL           Y N+   N+ +   DGW  TGD   Y + G+F    R+ E+IK +G  V P ELE L++  H  + +V VV V +++   L    VV+ ++ +  + I   + ++   +K++ G  ++  + LP+N  GK+LR E++
Sbjct:  273 FGVVKLFEVLKKCVP---NF---YGSTESGGGFLVSTNQHS----NGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQ 484          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000009272 (supercontig:LSalAtl2s:LSalAtl2s591:175091:177076:1 gene:EMLSAG00000009272 transcript:EMLSAT00000009272 description:"augustus_masked-LSalAtl2s591-processed-gene-1.0")

HSP 1 Score: 86.6557 bits (213), Expect = 4.926e-18
Identity = 119/506 (23.52%), Postives = 200/506 (39.53%), Query Frame = 0

Query:  121 RKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACE----------IYPGIEKIIVLGMEDKPADCLSFIDMLIYDDG-----SLYDQDRSC-----DVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETT--VAVLPFFHIYAMTAIMNL---GLHLGAK----IVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFL--------STNPAVK------AHHLASLESVIGGAAPFGPTLIEKFMEKC---------------SPSVVNFRE---------GFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQ---EGELCVAGPQVMKGYYKNKKATNKTIV--DG--WLLTGDIAKYDDNGQFMIVDRLKELIKVK-GLQVAPSELEDLIRRHSGVN-----------DVAVVGVPDE 540
            + F   L  LG +  + + +      ++ I+ LG      TL T+      + +A  L  + AK +IT    LP ++   +          I   +EK+ +    D  ++  S +++  + +      S Y  D  C         D+ ++ ++SG+TG PKG  L+H N++S ++     G N L          PE    +A LP  HI+  T  M +   G  +G      +          ++      KPT +  VP ++  +        S   A K       +   S+   +G   P    LI K + +                SP V +F E         G+G+TES     +    +  +G  G+P+P    K+++ E G     N     GE+ V G  V  GYY   + T ++    DG  W  TGDI +    G F I+DR K+L+K++ G  V+  ++E   + H  V+           D+  V VPD+
Sbjct:   54 QSFAHGLRYLGFQPREKIVIYAETSAKWLISALGCFRNAYTLVTVYTNLGEDGVAYSLNQTDAKVVITSEDLLPKLKGILKKGGHIHTIIYIEKPLEKMNIHAQNDDGSEQASEVNVYSFTEVIRIGVSSYSPDLDCYESWKATPEDIAIIMYTSGSTGNPKGALLSHANILSGILACCPYGXNLL--------VPPEEDRYIAYLPLAHIFEFTLEMTMILRGTSIGYSGPNTLTDAGXMIKRGHMGDARALKPTSMIAVPVILDRIFKGIQFKVSQEGAFKENLFTFCYQYRSMWRKLGFDTPIMNKLIFKKVRESLGGEIQSIIGGGAPLSPEVHDFLETVLCVKISQGYGLTESCGGITLCHYDDYHVGEVGYPLPGAYIKLVNWEEGGYKVNNSTAPRGEIHVGGNVVAMGYYNMSEKTKESFYEKDGIRWFPTGDIGQLQPTGAFKIIDRKKDLVKLQMGEYVSLGKVEGAWKIHPFVDAICSYGNSFKFDIVAVVVPDK 551          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 78.5666 bits (192), Expect = 1.509e-15
Identity = 125/521 (23.99%), Postives = 215/521 (41.27%), Query Frame = 0

Query:  110 QYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLE------NSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPA-DCLSFIDMLIYDDGS---LYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMT-AIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVK----AHHLAS---LESV-----IGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIVDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLI-RRHSGVNDVAVVGVPDERSGELPRAYVVRKN-------RNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRREL 599
            +YT   A+ +S + G+A             +++ N   F I  +G   +G +++   P+   E I RQL       N +   +IT    +P I        G+++II L     P    +S  ++ + DD     +++++ +  + +++  + +S+          L   +  S +   +H  + N +   P+S       + +  FFH    T  + N+   L  K   +    P EY+   +TH    +    P +   S   AVK    ++ L+S   L SV     IGG+     T  EK      PS+    +G+G TE+  +S    +++      G   P    +I++   G  LGPN  G++ V     M+ Y  + +       DG+   GD+  YD  G+     R K+L+KV    V P E+E  I  + S V D+ V G  D+   E     +V  +       +NVLE+    + A +   +K L  GV     +P+N  GKI R  L
Sbjct:   77 KYTKRFAHFISERHGNARF--------ATVLLLNNTRFFFIPVVGTWFLGSSVSLFQPSSSRETIERQLMILKEELNLEEIVVITETFAIPKISNI-----GVDRIISL----TPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENI-------LNSIHYCSYVPFQIHHVIKNYE---PSSLR----LMILTNFFHPGGFTEGLRNI---LPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSIN---TNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQH-----LGRLFPKYQLRILN-PNGEPLGPNSVGDIVVKPGYGMRRYIGDHEYN--ITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLER---TFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 345.895 bits (886), Expect = 1.466e-110
Identity = 208/550 (37.82%), Postives = 314/550 (57.09%), Query Frame = 0

Query:   64 AEDEDKIVRSPYSDCELVES-NLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQ-ACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVY-NDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTV-AVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPV-----SHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVR-KNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            A +E  + RS   D E+  S  L ++ + ++ +      +I G TG  YTY      SR+  + L R+G  KGDV+  ++ N PEF  +FLGAA +G   TT NP Y P E+ RQ E + A+ I+T    +  +R+ A E   G+  + V G  D    C+ F ++L  ++      D   DV+ +D+V LP+SSGTTG PKGV LTH ++++++ Q        +   NP    S +  +  +LP FHIY++ +++  GL  G+ IV + KF+    +  +  H  T    VPP+V  ++ +P V A  LAS+  V+ GAAP G  L + FM K   +V+   +G+GMTE+ PV     +  +       G CG  + N   KI+D +TG +LG NQ GE+C+ G Q+MKGY  + +AT  TI  DGWL TGDI   DD+ +  IVDRLKE+IK KG QV P+ELE L+  H  + D AVV + D+ +GE+P A++VR +   + E +I  +VA+    +K++   V F D++PKN +GKILR++L+A+
Sbjct:    5 AAEEVVVFRSKLPDIEIDNSMTLQEYCFARMAEVGARPCLIDGQTGESYTYAEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFAAE--RGVPVVTVDGAFDG---CVEFREVLAAEE-----LDADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQ-------QVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLG--QGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKRINK-VFFTDSIPKNPSGKILRKDLRAR 534          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|12229666|sp|Q9S777.1|4CL3_ARATH (RecName: Full=4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumarate--CoA ligase isoform 3; Short=At4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 340.117 bits (871), Expect = 2.654e-108
Identity = 204/558 (36.56%), Postives = 322/558 (57.71%), Query Frame = 0

Query:   57 YTPPTIPAEDEDKIVRSPYSDCELVES-NLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVY-NDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQ--------PEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRN-VLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            ++PPT       +I RS   D ++     L  + ++K+    D   +I G TG+ YTY   + + R+  S L +LG +KGDV+ +++ N  EF  +F+GA+ +G   TT NP Y  +E+ +QL++S AK IIT   ++  ++   E        ++   E  P +CL F  ++  D+ + + +  + D+  +D   LPFSSGTTG PKGV LTH ++++++ Q +     NL         S +  + VLP FHIY++ +++   L  GA ++ + KFE    L  +  H+ T   LVPPLV  L+ NP V ++ L+S+  V+ GAAP G  L +    +   +++   +G+GMTE+ PV  +         P K+   G CG  + N   K++ +ET  +LG NQ GE+C+ G Q+MK Y  + +AT+ TI  +GWL TGDI   D++ +  IVDRLKE+IK KG QV P+ELE L+  H  + D AVV   DE +GE+P A+VVR N N + E+D+ ++VA++   +K+L   V FV ++PK+ +GKILR++LKA+
Sbjct:   27 HSPPT------PRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGE-----NLTLITTDEPTPENCLPFSTLITDDETNPFQE--TVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLY------LKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILG--QGYGMTEAGPVLSMSLGFAKEPIPTKS---GSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHK-VFFVASIPKSPSGKILRKDLKAK 559          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|182627620|sp|Q54P79.2|4CL3_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 337.806 bits (865), Expect = 1.441e-107
Identity = 192/545 (35.23%), Postives = 301/545 (55.23%), Query Frame = 0

Query:   73 SPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFI-ITIGLFLPNIRQACEIYPGIEKIIVL---GMEDKPADCLSF--------IDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVV-RKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQN 603
            S Y +  + E  +   + K +   PD + ++ G+T ++Y+         K    L +L  KKGDV+G+++PN+PE+   F G   +G   + +NP Y  EE++  L     +++ +T+ ++         ++P +EK+I++   G   K  D L+          + L  ++G  Y   R  D+  D  ++PFSSGTTG  KGV L+H+N+VSN  Q         +    ++    ++ + VLPFFH + +   + L +  G +IVTLPKFEP  +L+ +  +K     +VPP+    + +P V    L+SL ++  GAAP G  + +   E+    +V  ++G+G TE SP   V P      G  G  +PN +AKII  ETG  LG  ++GE+C+ GP VM GYY N+KATN+ I  DG+L TGDI   D++G F I+DR KELIK KG QV P+ELE L+  H  V D  VVG+     GE+PR +VV ++N ++ E++++DW   + A +K    G+ F+  +PK+ TGK+LR+ LK  N
Sbjct:   13 SKYPNIIIPEKPIPHLILKHIRYKPDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLTNNNGKDYPIVR-IDLTKDTAIIPFSSGTTGLFKGVCLSHYNLVSNTYQT--------QTIETSTYKKNDSVIGVLPFFHSFGLMLHIMLMVKQGYRIVTLPKFEPVRFLELIKKYKVAMSFIVPPIAIMFAKSPIVDKFDLSSLRTLFCGAAPLGSEIEDLIKERFKGRLV-IKQGYGATELSPCCFVTPNGLVKSGSSGTLLPNLLAKIISSETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYFFIIDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFN 547          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|74897041|sp|Q54P77.1|4CL1_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 337.806 bits (865), Expect = 1.552e-107
Identity = 194/549 (35.34%), Postives = 301/549 (54.83%), Query Frame = 0

Query:   73 SPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFI-ITIGLFLPNIRQACEIYPGIEKIIVL---GMEDKPADCLSF--------IDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVV-RKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQNPGAL 607
            S Y +  + E  +   + K +   PD + ++ G+T ++Y+         K    L +L  KKGDV+G+++PN+PE+   F G   +G   + +NP Y  EE++  L     +++ +T+ ++         ++P +EK+I++   G   K  D L+          + LI ++G  Y   R  D   D  ++PFSSGTTG  KGV L+H N+VSN  Q         +    ++    +T + +LPFFHIY +   + L +  G ++V LPKFEP  +L+ +  +K     +VPP+    + +P V    L+SL ++  GAAP    + +   E+    +V  ++G+G TE SP   V P      G  G  +PN +AKII  ETG  LG  ++GE+C+ GP VM GYY N+KATN+ I  DG+L TGDI   D++G + IVDR KELIK KG QV P+ELE L+  H  V D  VVG+     GE+PR +VV ++N ++ E++++DW   + A +K    G+ F+  +PK+ TGK+LR+ LK  NP  L
Sbjct:   13 SKYPNIIIPEKPVPQLILKHIRSKPDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLINNNGKDYPIVR-IDPKKDTAIIPFSSGTTGLFKGVCLSHHNIVSNTYQT--------QTIETSTYKKNDTVMGILPFFHIYGLMLFLMLMVKQGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKFDLSSLRTLFSGAAPLSSEVEDLIKERFKGRLV-IKQGYGATELSPACFVIPSGLVKSGSAGILLPNQLAKIISPETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFNPPKL 551          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|112800|sp|P14912.1|4CL1_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 332.413 bits (851), Expect = 1.372e-105
Identity = 203/547 (37.11%), Postives = 316/547 (57.77%), Query Frame = 0

Query:   64 AEDEDKIVRSPYSDCELVES-NLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPV-----SHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNR-NVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            A  ED I RS   D  + +   L  + ++ + +  D   +I G TG  +TY     +SRK  S L +LG ++GD + +++PN PE+  AFLGA+  G   T  NP +   E+ +QL+ SQAK IIT   ++  ++     Y   + I ++ ++D P DCL F  ++  D+  + +   + D   D+V LP+SSGTTG PKGV LTH  +V+++ Q +     NL         S +  + +LP FHIY++ A++  GL  G  I+ + KF+   +L+ +  +K T    VPP+V  ++ +P V  + L+S+ +V+ GAAP G  L +    K   +     +G+GMTE+ PV     +  +       G CG  + N   KI+D ET A+L  NQ GE+C+ G Q+MKGY  + ++T  TI  +GWL TGDI   DD+ +  IVDRLKE+IK KG QVAP+ELE L+  H  ++D AVV + DE++GE+P A+VVR N     E++I  +V+++   +K++   V FVD +PK+ +GKILR++L+A+
Sbjct:    6 APKEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLIITQACYVDKVKD----YAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSD---DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNL------YMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNA--KLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRI-FRVFFVDAIPKSPSGKILRKDLRAR 536          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|75289194|sp|Q67W82.1|4CL4_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 4; Short=4CL 4; Short=Os4CL4; AltName: Full=4-coumaroyl-CoA synthase 4)

HSP 1 Score: 333.183 bits (853), Expect = 1.387e-105
Identity = 205/543 (37.75%), Postives = 305/543 (56.17%), Query Frame = 0

Query:   70 IVRSPYSDCEL-VESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVY-NDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTV-AVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPV-----SHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVR-KNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            + RS   D E+     L  + ++K+ +      +I G TG  Y+Y     +SR+  + L RLG  KGDV+  ++ N PEF   FLGAA +G   TT NP Y P EI RQ   + A+ I+T    +  +R     +     I V+ ++     C+ F + ++  D S+   D   +V+ +D+V LP+SSGTTG PKGV LTH ++V+++ Q        +   NP      E  V  +LP FHIY++ +++  GL  G+ IV + KF+    +     H  T    VPP+V  ++ +P V A  LAS+  V+ GAAP G  L + FM K   +V+   +G+GMTE+ PV     +  +       G CG  + N   KI+D +TGATLG NQ GE+C+ G Q+MKGY  + ++T  TI   GWL TGDI   DD+ +  IVDRLKE+IK KG QV P+ELE L+  H  + D AVV + DE +GE+P A++VR +   + E +I  +VA+    +K+L   V F D++PK+ +GKILR++L+A+
Sbjct:   18 VFRSKLPDIEIPSHLTLQAYCFEKLPEVAARPCLIDGQTGAVYSYGEVEELSRRAAAGLRRLGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEIHRQASAAGARVIVTEACAVEKVRG----FAADRGIPVVAVDGDFDGCVGFGEAML--DASIEPLDADEEVHPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQ-------QVDGENPNLYFRREDVVLCLLPLFHIYSLNSVLLAGLRAGSAIVIMRKFDLGALVDLTRRHGVTVAPFVPPIVVEIAKSPRVTADDLASIRMVMSGAAPMGKDLQDAFMAKIPNAVLG--QGYGMTEAGPVLAMCLAFAKEPFEVKSGSCGTVVRNAELKIVDPDTGATLGRNQSGEICIRGEQIMKGYLNDPESTKNTIDKGGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPDIKDAAVVPMIDEIAGEVPVAFIVRIEGSAISENEIKQFVAKEVVFYKRLNK-VFFADSIPKSPSGKILRKDLRAK 544          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|112801|sp|P14913.1|4CL2_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 329.331 bits (843), Expect = 2.822e-104
Identity = 202/547 (36.93%), Postives = 314/547 (57.40%), Query Frame = 0

Query:   64 AEDEDKIVRSPYSDCELVES-NLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPV-----SHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNR-NVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            A  ED I RS   D  + +   L  + ++ + +  D   +I G TG  +TY     +SRK  S L +LG ++GD + +++PN PE+  AFLGA+  G   T  NP +   E+ +QL+ S AK IIT   ++  ++     Y   + I ++ ++D P DCL F  ++  D+  + +     D   D+V LP+SSGTTG PKGV LTH  +V+++ Q +     NL         S +  + +LP FHIY++ A++  GL  G  I+ + KF+   +L+ +  +K T    VPP+V  ++ +P V  + L+S+ +V+ GAAP G  L +    K   +     +G+GMTE+ PV     +  +       G CG  + N   KI+D ET A+L  NQ GE+C+ G Q+MKGY  + ++T  TI  +GWL TGDI   DD+ +  IVDRLKE+IK KG QVAP+ELE L+  H  ++D AVV + DE++GE+P A+VVR N     E++I  +V+++   +K++   V FVD +PK+ +GKILR++L+A+
Sbjct:    6 APKEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLIITQACYVDKVKD----YAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSD---DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNL------YMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNA--KLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRI-FRVFFVDAIPKSPSGKILRKDLRAK 536          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 328.561 bits (841), Expect = 4.564e-104
Identity = 188/525 (35.81%), Postives = 306/525 (58.29%), Query Frame = 0

Query:   85 LADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPV-----SHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQD-IIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            L  + ++ + ++     +I G   + YTY       RK    L +LG ++ D + +++PN PEF  AF+GA+ +G   T  NP + P E+ +Q + S AK IIT   F+  ++     Y     + V+ ++  P  CL F ++   D+  + +     D   D+V LP+SSGTTG PKGV LTH  +V+++ Q +     NL         S +  + VLP FHIY++ +I+  GL +GA I+ + KF+   +L+ +  +K +    VPP+V  ++ +P V ++ L+S+ +V+ GAAP G  L +    K   +     +G+GMTE+ PV     +  +   +   G CG  + N   KI+D +TG +L  NQ GE+C+ G Q+MKGY  + +AT +TI  +GWL TGDI   D++ +  IVDRLKELIK KG QVAP+E+E L+  H  ++D AVV + DE++GE+P A+VVR N + + +D + D+++++   +K++   V FV+T+PK+ +GKILR++L+A+
Sbjct:   32 LHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKIIITQSCFVGKVKD----YASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPD---DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANL------YMHSEDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTKFPNA--KLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKR-VFFVETVPKSPSGKILRKDLRAR 540          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 326.25 bits (835), Expect = 3.483e-103
Identity = 193/546 (35.35%), Postives = 316/546 (57.88%), Query Frame = 0

Query:   68 DKIVRSPYSDCELVES-NLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVY-NDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQ--------PEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            + + RS   D E+ +   L  + +++V ++ D   +I G T R Y +     +SRK  + L +LG ++G V+ +++PN  EF   F+GA+  G  +TT NP Y+P EIA+Q + + A+ I+T+  ++  +            ++V+ ++D P +    I +L   D +   Q  +  ++ +D+V LP+SSGTTG PKGV LTH  +VS++ Q +     NL         S +  + VLP FHIY++ +++   L  GA  + + KF     L+ +  +K T   +VPP+V  ++ +P V  + ++S+  ++ GAAP G  L +   E+   ++  F +G+GMTE+ PV  +         P K+   G CG  + N   KI+D ETG +L  NQ GE+C+ GP++MKGY  + ++T  TI  +GWL TGD+   DD+ +  IVDR+KE+IK KG QVAP+ELE L+  H  + D AVV    E +GE+P A+VV K+  + EQ+I ++VA++   +KK+   V FVD +PK+ +GKILR++L+++
Sbjct:    9 EHLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQS-----HDVLVITIDDAPKEGCQHISVLTEADET---QCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNL------YFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAI--FGQGYGMTEAGPVLAMNLAFAKNPFPVKS---GSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVV-KSSEISEQEIKEFVAKQVIFYKKIHR-VYFVDAIPKSPSGKILRKDLRSR 533          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. SwissProt
Match: gi|148841518|sp|Q9S725.2|4CL2_ARATH (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumarate--CoA ligase isoform 2; Short=At4CL2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 326.25 bits (835), Expect = 5.422e-103
Identity = 200/535 (37.38%), Postives = 306/535 (57.20%), Query Frame = 0

Query:   85 LADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQ--------PEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVR-KNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQNPGALVS 609
            L D++++ + ++     +I G TG  YTY   +  SRK  + L  LG K+ DV+ +++PN PE  + FL A+ +G   T+ NP + P EI++Q + S AK I+T   ++  I+       G+  I+    +  P +CL F ++   ++  + D         D+V LPFSSGTTG PKGV LTH  +V+++ Q +     NL           +  + VLP FHIYA+ +IM   L +GA I+ +PKFE    L+ +   K T   +VPP+V  ++ +P  + + L+S+  V  GAAP G  L +    K   +     +G+GMTE+ PV  +         P K+   G CG  + N   KI+D +TG +L  N+ GE+C+ G Q+MKGY  +  AT  TI  DGWL TGD+   DD+ +  IVDRLKELIK KG QVAP+ELE L+  H  +NDVAVV + +E +GE+P A+VVR K+ N+ E +I  +V+++   +K++   V F D++PK  +GKILR++L+A+    L++
Sbjct:   38 LHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQN--DGV-LIVTTDSDAIPENCLRFSELTQSEEPRV-DSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNL------YFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNA--KLGQGYGMTEAGPVLAMSLGFAKEPFPVKS---GACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINK-VFFTDSIPKAPSGKILRKDLRARLANGLMN 556          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|1325315469|ref|XP_023339433.1| (4-coumarate--CoA ligase 2-like [Eurytemora affinis])

HSP 1 Score: 777.704 bits (2007), Expect = 0.000e+0
Identity = 374/562 (66.55%), Postives = 455/562 (80.96%), Query Frame = 0

Query:   44 RGLTTARPLSAGHYTPPT---IPAEDEDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIVDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            RGL T   L A   + P    +P E E  IV+S +SD ++ E NLAD+VW+ +     + A++CGMTGR Y++ MA+G+S+KFGSALLR+GAKKGDV+G+V+PNIPEFPI F+GA GV VT+TTMNPTYRPEEIARQ+ENS+ KFI+TIG+F+ NI+Q+C+IY G+EKIIV+G    P  C+SF+D+++ +DGSLY QD++C+ + D+V LP+SSGTTGPPKGV LTH+N+V+N+ Q  HP    +K    T      +T+AVLPFFHIYAM  +M   L  G K +TLPKFEPE Y+KAL T+KPTFL+LVPPLVSFL+TNPAVK   L S+  V GGAAPFGP LIEKFMEK  P  + FREGFGMTESSP++H+QP+ +AVLG CG P+ NT AKI+D ETG  LGP ++GELCV+GPQVM+GY+KN  AT KTI++GWL+TGDIAKYD +GQF IVDRLKELIKVKGLQVAPSELEDLIRRHSGV DVAVVGVPDERSGELPRAY++RKNRNV EQ IID+VA+R APHKKLGAGVMFV+TLPKNQTGKILRRELKAQ
Sbjct:   32 RGLNTNNALHAVDRSQPNGVVLPGE-EGFIVKSRWSDVQIPEMNLADYVWEDIEHNAANPALVCGMTGRSYSFAMAHGLSKKFGSALLRMGAKKGDVIGLVLPNIPEFPIVFMGAVGVNVTVTTMNPTYRPEEIARQMENSETKFIVTIGMFVQNIKQSCDIYTGVEKIIVIGDAIAPEGCISFLDLIMGEDGSLYGQDKTCNPHEDVVALPYSSGTTGPPKGVCLTHYNLVANLCQAGHPDALPIKPFKETGVLG--STLAVLPFFHIYAMELVMLFNLRNGQKTITLPKFEPESYIKALDTYKPTFLNLVPPLVSFLATNPAVKPSLLNSVTFVTGGAAPFGPALIEKFMEKVHPHQIEFREGFGMTESSPLTHLQPDGDAVLGACGCPVSNTWAKIVDTETGEILGPEEQGELCVSGPQVMQGYFKNISATEKTIINGWLMTGDIAKYDKSGQFTIVDRLKELIKVKGLQVAPSELEDLIRRHSGVLDVAVVGVPDERSGELPRAYIIRKNRNVTEQSIIDYVADRVAPHKKLGAGVMFVETLPKNQTGKILRRELKAQ 590          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|321471668|gb|EFX82640.1| (hypothetical protein DAPPUDRAFT_316454 [Daphnia pulex])

HSP 1 Score: 528.865 bits (1361), Expect = 1.245e-179
Identity = 262/506 (51.78%), Postives = 353/506 (69.76%), Query Frame = 0

Query:  104 CGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSP----ETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNR-NVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQN 603
            CG+TGR+Y+Y     + R+FGSAL+R+G +KG+V GM++PN+PEFPI  LGAAG+G+ +TT+NPTY   EIARQL+NS    ++TI      +RQ  ++ P + +++VLG    P +  + I  L+ D G L+D+    D   D  VLP+SSGTTG PKGV LT+ N+ +N+ Q+ HPG   +  A PT+        E  V VLPFFHIY M ++M  GL  GAK+VTLP+FE E +L+ +  H+PT L LVPPLVS+LS  P +K      L ++I GAAP GP +    +E+     +  +EG+GMTE+S V+H+ P  N  +G  G P+  T  K++D+ETG +LGP Q+GE+CV GPQ+MKGY+ N+KAT +T+  DGWL TGDI  YD+  QF +VDRLKELIKVKGLQV+PSELED+IRRHSGV+DVAV+GVPDE +GELPRAYVV+K    V ++DI ++V  + APHKKL  GV+F+D++PK  TGK+LRRELK  N
Sbjct:   33 CGLTGRKYSYGKVRELVRRFGSALVRMGFRKGEVFGMILPNLPEFPIVLLGAAGIGMPVTTVNPTYTVGEIARQLQNSNTTVVVTIPQLTEALRQVAQLCPKLRRLVVLG---NPEEGFTSIGELLQDSGDLFDEKIEIDPSMDTFVLPYSSGTTGLPKGVLLTNSNVTANIQQIEHPGTMKVS-ACPTTNCDASDLQEVFVCVLPFFHIYGMVSVMLTGLDHGAKLVTLPRFESESFLENVHQHRPTMLQLVPPLVSYLSVRPDLKLESFRRLHTIIIGAAPLGPAVANMLIERLGKPDLLMQEGYGMTETSSVTHLSPIVNNKIGSFGEPLSRTQVKVVDVETGESLGPGQQGEMCVRGPQMMKGYHNNEKATKETVDSDGWLHTGDIVYYDEENQFFVVDRLKELIKVKGLQVSPSELEDVIRRHSGVSDVAVIGVPDEIAGELPRAYVVKKKGVAVSKEDIAEFVDVKVAPHKKLKGGVVFLDSIPKTNTGKLLRRELKHLN 534          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|321471685|gb|EFX82657.1| (hypothetical protein DAPPUDRAFT_316478 [Daphnia pulex])

HSP 1 Score: 527.709 bits (1358), Expect = 1.830e-178
Identity = 272/579 (46.98%), Postives = 378/579 (65.28%), Query Frame = 0

Query:   27 RAAFRLARPHPARVLQARGLTTARPLSAGHYTPPTIPAEDEDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTS---PETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVL-EQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELK 600
            R A +L +P     +Q R ++ A   S G+       +  +  IV S + D +L    +     ++   WP+  A  CG+TGR+YTY     + R FGSAL+R+G KKG+V GM++PN+PEFPI  LGAAG+G+ +TT+NP Y  EEIARQ++ S A  ++TI      +RQ  ++ P I ++I++G   +P +  +    ++ D G LYD++ + +   D+  LP+SSGTTG PKGV L+  N+ +N+ QLLHP    L    PTS  +    E  V +LPFFH+Y M  +M  GL LGAK+V LP+FE E Y+ +L  H P+ LHLVPPLV+FL   P +K      L +V  GAAP G  +  +F+E+     +  +EGFGMTE++   H+ P KN  +G  G P+P T  K+ID++TG +LGP Q GE+CV GPQ+MKGYYKN+KAT +T   +GWL TGD+  Y++  QF IVDRLKELIKVKGLQV+PSELED++RR  GV DVAV+GVPD+ +GELPRAYVV+K  N L ++DII++V  + + HK+L  GV+F+D++PK  TGKILRRELK
Sbjct:   29 RTAVQLLKPS----VQIRAVSCAT--SVGN-------SSIDKNIVYSRHQDFDLRTQTVVQRFIERASLWPNLTATECGLTGRKYTYGQLIVLIRHFGSALVRMGFKKGEVFGMLLPNLPEFPIVLLGAAGIGMPVTTVNPIYTVEEIARQMQLSGATVVVTIPQLAGTLRQVAQLCPEIRRLIIIG---QPEEGFATFGEMLQDSGDLYDENINVNPSEDIFALPYSSGTTGLPKGVMLSDANITANIQQLLHPTAMKLTSPPPTSNATDELQEVYVVILPFFHMYGMVGVMLTGLDLGAKMVILPRFEGESYVNSLHQHHPSTLHLVPPLVAFLGLRPDLKLEAFQRLHTVAIGAAPLGTAVATRFVERLGRPNLLMQEGFGMTETTSACHLSPVKNNQIGSFGEPLPRTHVKVIDVDTGESLGPGQPGEMCVQGPQMMKGYYKNEKATKETYDSEGWLHTGDMVYYNEQNQFFIVDRLKELIKVKGLQVSPSELEDVLRRIPGVLDVAVIGVPDDIAGELPRAYVVKKEGNPLSKEDIIEFVDAKVSHHKRLKGGVVFLDSIPKTNTGKILRRELK 591          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|1022770191|gb|KZS14605.1| (4-coumarate--CoA ligase 3 [Daphnia magna])

HSP 1 Score: 523.087 bits (1346), Expect = 1.538e-176
Identity = 267/547 (48.81%), Postives = 364/547 (66.54%), Query Frame = 0

Query:   60 PTIPAEDEDK-IVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTS---PETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVL-EQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELK 600
            P+  A   DK IV S + D +L    +    +++   WP+  A  CG+TGR+YTY     ++R FGSAL+R+G K+G+V GMV+PN+PEFPI  LGAAG+G+ +TT+NP Y  EEIARQL+ S A  ++TI      +RQ  ++ P I ++IV+G   KP +  +    ++ D G+L+D++   +   D+  LPFSSGTTG PKGV L++ N+ +N+ QLLHP    L    PT   +    E  V +LPFFH+Y M  +M  GL LGAK+V LP+FE + Y+ +L  H P+ LHLVPPLV FL   P +K      L +V  GAAP G  +  +F+E+     +  +EG GMTE++ V+H+ P  N  +G  G P+  T  K++D++TG +LGP Q GE+CV+GPQVMKGYYKN KAT +TI  DGWL TGD+  Y++  QF IVDRLKELIKVKGLQV+PSELED++RRH GV DVAV+GVPD+ +GELPRAYVV+K    L +++I ++V  + + HKKL  GV F+D +PK  TGKILRRELK
Sbjct:   47 PSGEASSIDKHIVYSRHKDFDLRSQTVVQRFFERASMWPNLTATECGLTGRKYTYGKLRDLTRNFGSALVRMGFKRGEVFGMVLPNLPEFPIILLGAAGIGMPVTTVNPIYNVEEIARQLQLSGATVVVTISELAGTLRQVAQLCPEIRRLIVVG---KPEEGFASFGEMLQDPGNLFDENIEINPMEDVFALPFSSGTTGLPKGVMLSNANVTANIQQLLHPTAMKLTTPPPTRHETDVLQEVYVCILPFFHMYGMVGVMLTGLDLGAKMVILPRFEGDSYVNSLHQHHPSTLHLVPPLVLFLGHRPDLKLDAFRRLHTVAIGAAPLGLAVATRFVERFQKHDLLMQEGCGMTETTSVTHLSPVINNQIGSFGEPLSRTKVKVLDVDTGESLGPGQHGEMCVSGPQVMKGYYKNPKATEETIDSDGWLHTGDMVYYNEQNQFFIVDRLKELIKVKGLQVSPSELEDILRRHPGVLDVAVIGVPDDAAGELPRAYVVKKPGVALSKEEIAEFVDAKVSHHKKLKGGVHFLDAIPKTNTGKILRRELK 590          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|1228407097|ref|XP_021962746.1| (probable 4-coumarate--CoA ligase 1 [Folsomia candida])

HSP 1 Score: 514.998 bits (1325), Expect = 8.819e-174
Identity = 258/540 (47.78%), Postives = 361/540 (66.85%), Query Frame = 0

Query:   65 EDEDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDML-IYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQP--EKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELK 600
            +D   +V S ++   +V   + +F W  +H+W ++ A  CG++GR+YT+D  Y  SR F +AL + G K+ DV+ +++PN+PE+    LG    GVT +  NPTY   EIA QL N +AK I+T+ +FLP +++A    P ++ ++V+G E +P  C S+ +M  +  DG  + +    D  N   +LP+SSGTTGPPKGV LTH N+ +N++Q+  PG   L     T     +  + +LPFFHIY    +M   L+ GA  +TLPKFEP+ ++ ++ +H+P FLHLVPPL+SF++ +P VK   L S+  ++GGAAP GP +I + +EK     + F EG+GMTE SPVSHV P    N  +G CG P+P ++ K++D+ETG  LGPNQ GELC+ GPQ+MKGYYKN+KATN+TI   GWL TGDI  YD++  F IVDRLKELIKV+G QVAPSELEDL+R+H  V DVAV+GVPDER+GELPRAY+V K+    E+ I  ++  + +P+KKL  GV FV  +PK  +GKILRRELK
Sbjct:   32 DDRFCVVPSAHNLDPVVGVTIPEFFWSNLHKWGNATAFECGVSGRKYTHDQVYKQSRSFAAALKQHGLKENDVVAILLPNVPEYAAILLGILEAGVTASPFNPTYTAHEIATQLNNCEAKAIVTLPMFLPQVQEAKAQCPLLKSVVVVG-EAQPG-CHSYFEMAKVNSDGVEFLKGSKIDTVNHTAILPWSSGTTGPPKGVMLTHSNINTNLLQVTQPGY--LATKPFTGGNEQDKFIGILPFFHIYGFLLVMAKTLYHGAHTMTLPKFEPKSFIDSVRSHQPNFLHLVPPLISFMANSPDVKTEDLKSIACIMGGAAPIGPVIINRVLEKAGKHFL-FTEGYGMTELSPVSHVSPVATNNKKIGSCGVPVPGSLCKVVDVETGEALGPNQPGELCIKGPQLMKGYYKNEKATNETIDAKGWLHTGDIGLYDEDKYFFIVDRLKELIKVRGFQVAPSELEDLLRKHPEVTDVAVIGVPDERAGELPRAYIVSKSSKPSEESIHKYLETKVSPNKKLKGGVEFVQAIPKAASGKILRRELK 566          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|321471684|gb|EFX82656.1| (hypothetical protein DAPPUDRAFT_316477 [Daphnia pulex])

HSP 1 Score: 515.383 bits (1326), Expect = 1.551e-173
Identity = 265/540 (49.07%), Postives = 361/540 (66.85%), Query Frame = 0

Query:   67 EDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSP---ETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNR-NVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            +  IV S + D +     +   +++K   WP+  AM CG+TGR+YTY     + R+FGS+L R+G KKG+V G+++PN+PEFPI  LGAA +G+ +TT+NPTY  EEIARQ + S A  ++T       +RQ  ++ P I ++IV+G    P +  + I+ +  D G L+D++       D+ VLPFSSGTTGPPKGV LTH N+ SNM Q+ HPG   +     TS  S    E  + VLPFFH+Y M  +M  GL  GAK+VTLP+FE   YL ++  H+P+ LHL PPLVS L   P +K      L +VI GAAP GP    +F+E+     +  +EG+GMTE+S V HV P  N+ +G  G P+  T  K++D+ TG +LGP Q GE+C+ GPQ+MKGYYKN+KAT +TI  DGWL TGD+A Y++  QF IVDRLKELIKVKG QVAPSELED++RR  G+ DVAV+GVPD+ +GELPRAYVV+K   ++ E++II++V  + + HK+L  GV+F+D +PK  TGKILRRELK+
Sbjct:   57 DKNIVYSRHQDFDFRSQTVIQRIFEKASLWPNLTAMECGLTGRKYTYGQLIQLIRRFGSSLTRMGFKKGEVFGIILPNLPEFPIVLLGAAAIGMPVTTVNPTYTVEEIARQFQLSGATAVVTTPQLAETLRQVAKLCPEIRRLIVIG---NPEEGFASIEDMFQDSGDLFDENIDIIPSEDIFVLPFSSGTTGPPKGVMLTHSNLCSNMAQVGHPGTMKVAYCPATSSDSSDLQEVLICVLPFFHMYGMLLVMLSGLDHGAKLVTLPRFESASYLNSVHQHRPSALHLAPPLVSLLGLRPDLKLEAFHRLHTVIVGAAPLGPAAATRFIERLRKPDLFMQEGYGMTETSCVCHVSPIVNSQIGSFGEPLSRTQVKVVDLVTGLSLGPGQHGEVCIKGPQLMKGYYKNEKATKETIDNDGWLHTGDMAYYNEQNQFFIVDRLKELIKVKGFQVAPSELEDILRRIPGILDVAVIGVPDDIAGELPRAYVVKKESISLTEKNIIEFVDAKVSHHKRLKGGVVFLDVIPKTATGKILRRELKS 593          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|321463518|gb|EFX74533.1| (hypothetical protein DAPPUDRAFT_307209 [Daphnia pulex])

HSP 1 Score: 501.901 bits (1291), Expect = 8.709e-170
Identity = 258/485 (53.20%), Postives = 339/485 (69.90%), Query Frame = 0

Query:  119 MSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIVD-GWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNR-NVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            ++R+FGSAL+R+G KKG+V+G+V+PN+PEFPI  LGAAG+G+ +TT+NP Y  EEIARQ++NS    I+TI      IR+   + P I ++IV+G    P +    +  +  D G L+D++   D ++D+ VLP+SSGTTG PKGV LTH N+ +N+ Q++HPG   +     TS +  E  + VLPFFHIY M   M  GL  GAK+VTLP+FE E +L  L  H PT L LVPPLVSFL   P +K      L +V  GAAP GP    K +++     +  +EG+GMTESSPV+H+ P  N  +G  G P+  T  KI+D+ TG +LGP QEGE+CV GPQVMKGYYKN+KAT +TI   GWL TGDIA Y++  QF IVDRLKELIKVKGLQV+PSELED++RRH  V DVAV+G+PD+ SGELPRAYVV+KN  +V ++DI ++V  + APHK+L  GVMF+D +PK  TGK+LRRELK+
Sbjct:    2 LTRRFGSALVRMGFKKGEVLGLVLPNLPEFPITMLGAAGIGMPVTTVNPIYTAEEIARQMQNSGTSVIVTIPQMADTIREVASLCPSIRQLIVVG---GPQEGFVSLMEMFQDSGDLFDENIELDPFDDMFVLPYSSGTTGLPKGVMLTHSNICANIQQIIHPGTARVIPT--TSNSFQEVYICVLPFFHIYGMVGSMLTGLDHGAKLVTLPRFESESFLNCLYQHHPTMLQLVPPLVSFLGHRPDLKLEAFHRLHTVFCGAAPLGPAAANKLVDRLGRRDILMQEGYGMTESSPVTHMSPIINNEIGSFGEPVSRTRVKIMDLNTGESLGPGQEGEMCVFGPQVMKGYYKNEKATEETIDSAGWLHTGDIAYYNEQNQFFIVDRLKELIKVKGLQVSPSELEDVLRRHPAVLDVAVIGIPDDMSGELPRAYVVKKNGVSVSKEDIAEFVDAKVAPHKRLKGGVMFIDAIPKTNTGKLLRRELKS 481          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|1061481214|gb|ODM98582.1| (4-coumarate--CoA ligase 2 [Orchesella cincta])

HSP 1 Score: 502.286 bits (1292), Expect = 1.117e-168
Identity = 250/520 (48.08%), Postives = 349/520 (67.12%), Query Frame = 0

Query:   87 DFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDML-IYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETT-VAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQP--EKNAVLGGCGHPIPNTMAKIIDIETGATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKNRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 601
            +F+W  +H+W D+ A  CG++GR+YT+ M   +SR FG+AL + G K+ DV+ +++PN+PE+PIA LGA    + ++ +NP Y P EIA+Q+  SQ+K II +  F+P +R+  ++   ++ IIV+G  D    C SF +M+ +   G  + +    D  ++L +LP+SSGTTGPPKGV L+H N+ SN+ Q+  PG     E N   K   +   + +LPFFH +  T +M   L+ GA   TLPKFEP  ++KAL  HKP  +HLVPPL++F++ +P V  +   S+ +++GGAAP G  LI + +EK       F+EG+GMTE SPVSH+ P   KN  +G  G  +P T  KI+D  TGA LG NQ GE+CV GPQ MKGY+KN KAT +TI  +GWL TGDI  +D+   F IVDR+KELIKVKGLQVAPSELED++R H  V DVAV+G+PDER+GELPRAY+VRK   + E ++ +++AE+ + HKKL  GV F+  +PK  +GKILRRELKA
Sbjct:   63 EFIWSNLHKWEDAPAFECGVSGRKYTHGMINKLSRSFGAALKQHGLKRHDVVCLLLPNVPEYPIATLGAWEASLVVSPINPVYTPAEIAKQVNGSQSKVIIALSQFVPTVREVQKLSSSLKSIIVIG--DAVDGCHSFSEMVKVNSQGVEFARGSQFDTTDELALLPYSSGTTGPPKGVMLSHSNIASNLFQMTRPG---YVETNFFEKGDEQDRFIGILPFFHSFGFTVVMAKTLYQGAFTRTLPKFEPPTFIKALREHKPKCVHLVPPLIAFMANSPDVTKNEFVSIRAIMGGAAPIGEALITRLLEKAGKYFF-FQEGYGMTEMSPVSHILPGYTKNTKIGSTGVILPGTQCKIVDPSTGAALGANQRGEICVKGPQNMKGYFKNDKATAETIDSEGWLHTGDIGLFDEGECFWIVDRMKELIKVKGLQVAPSELEDMLRTHPDVADVAVIGIPDERAGELPRAYIVRKKETLSENEVKEFIAEKVSDHKKLAGGVEFLPAIPKAASGKILRRELKA 576          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|321456296|gb|EFX67408.1| (hypothetical protein DAPPUDRAFT_203687 [Daphnia pulex])

HSP 1 Score: 501.13 bits (1289), Expect = 6.716e-168
Identity = 251/540 (46.48%), Postives = 354/540 (65.56%), Query Frame = 0

Query:   67 EDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETG-ATLGPNQEGELCVAGPQVMKGYYKNKKATNKTI-VDGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRK--NRNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            E+ IV SPY DC+L +  +   +++   +WP+ IA+ CG+TGR+YTY+M   + R+FGSAL R+  KKG+V G++ PNIPEFPI   GA+G G+ ++ +NPT+  EE+ARQL    A  +  +      ++Q   + P I  II+LG    P +       +  D G L+++    +   D+ +LP SSGT+G PK V L+HFNM SN++Q L PG  N + A    +   +T + +LPFFH Y +T +MN G   GAK+VTLP+FE + YLKA+  HKPT +H+VPPL + L+ +PA+K   L+ + ++  GAAP G     K +E+ +   ++ +EG+G++E+SP   + P  N  LG CG PI  + AK+I+ E G   LGP Q GEL V+GPQVMKGY+ N KAT + I  +GWL TGD+A YDD+G F IVDRLKELIKVKGLQVAP+ELED++  H  V +VAV+G+PDE +GELPRAYVV+K    +V + DI  +V  + +PHK++  G+ F  T+PKN  GKILRREL+ Q
Sbjct:   47 ENNIVTSPYGDCDLHDMTMVQKIFESAGRWPNKIALECGITGRKYTYEMTCQLIRRFGSALTRMEYKKGEVFGIISPNIPEFPIVLYGASGAGMPVSLVNPTFTAEEMARQLSAVGATALFGVAPMAETLKQVARLCPVIRHIILLG---PPLEGAVSFQQMAQDSGDLFNETLDAE---DVFLLPHSSGTSGLPKSVMLSHFNMTSNVMQFLQPGGTNHQVATTNYQ---DTYICLLPFFHAYGITLLMNTGFQTGAKLVTLPQFEVQSYLKAIDDHKPTAMHVVPPLATLLAQHPALKVESLSQMHTIFCGAAPLGVQTSVKLLERLNNPNLSLQEGYGLSETSPGVLMAPLGNTKLGSCGAPISRSKAKVINHEIGDQALGPYQHGELYVSGPQVMKGYWNNPKATEEMIDGEGWLRTGDVAYYDDDGNFYIVDRLKELIKVKGLQVAPAELEDVLTSHPAVGEVAVIGIPDEHAGELPRAYVVKKPGMESVSDSDIQAFVDSKVSPHKQIKGGIEFCATIPKNNMGKILRRELRVQ 577          

BLAST of hypothetical protein DAPPUDRAFT_316454 vs. nr
Match: gi|1022764784|gb|KZS10131.1| (Long-chain fatty acid transport protein 1 [Daphnia magna])

HSP 1 Score: 483.411 bits (1243), Expect = 4.180e-161
Identity = 248/557 (44.52%), Postives = 347/557 (62.30%), Query Frame = 0

Query:   50 RPLSAGHYTPPTIPAEDEDKIVRSPYSDCELVESNLADFVWKKVHQWPDSIAMICGMTGRQYTYDMAYGMSRKFGSALLRLGAKKGDVMGMVVPNIPEFPIAFLGAAGVGVTLTTMNPTYRPEEIARQLENSQAKFIITIGLFLPNIRQACEIYPGIEKIIVLGMEDKPADCLSFIDMLIYDDGSLYDQDRSCDVYNDLVVLPFSSGTTGPPKGVSLTHFNMVSNMVQLLHPGVNNLKEANPTSKTSPETTVAVLPFFHIYAMTAIMNLGLHLGAKIVTLPKFEPEEYLKALSTHKPTFLHLVPPLVSFLSTNPAVKAHHLASLESVIGGAAPFGPTLIEKFMEKCSPSVVNFREGFGMTESSPVSHVQPEKNAVLGGCGHPIPNTMAKIIDIETG-ATLGPNQEGELCVAGPQVMKGYYKNKKATNKTIV-DGWLLTGDIAKYDDNGQFMIVDRLKELIKVKGLQVAPSELEDLIRRHSGVNDVAVVGVPDERSGELPRAYVVRKN--RNVLEQDIIDWVAERAAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 602
            +PL+AG      I +  E  +V S +SDC L    +    +  V ++   IA+ CG+TGR+YTY+M   + R++GSAL R G KKG VM +V PN+PEF IA LGA+ +G+ +  +NPT+ P EIARQL    A  +  +      ++Q  ++ P + ++I+LG    P +       ++ D G L++++   DV ND  VLP SSGTTG PK V LTHFN+ SN+ Q +HPG +  + A    +   ET + +LPFFH Y +  IM +G  LGA++VTLP+F+   YLKA+  +KPT LH+VP L      +PA+K      L ++   A+P G     +  EK +   +  ++G+G+TE+SP     P  N   G CG  I  T AK+ID+E G  TLGPNQ+GEL +AGPQ+MKGYY   +AT + I  DGWL TGD+A YD+ G F IVDRLKELIKVKG QVAP+ELED++  H  + + AV+G+PDE +GELPRAYVV+K    +V + +I  +V  + + HK++  GV F D LPKN  GK+LRREL+AQ
Sbjct:   42 QPLTAGDVA---INSRIETNMVSSRHSDCPLHGMTMVHRFFDNVSRYSKQIALECGITGRKYTYEMLAHLIRRYGSALTRAGFKKGQVMAIVSPNVPEFAIALLGASAIGMPVALVNPTFTPAEIARQLSTVGATAVFGVAPMAELLKQVAQLCPSVRRVILLG---PPQEGFVCFQEMLQDSGDLFNENVDIDVMNDTFVLPHSSGTTGVPKNVMLTHFNVGSNVTQNIHPGTSRNQLATDQYQ---ETHICLLPFFHSYGVNGIMMIGFDLGARLVTLPRFDAASYLKAIDDYKPTLLHIVPTLAYLFIKHPALKMDGFERLHTIFCAASPLGEKTATQLQEKFNRPDLLIQQGYGLTETSPGVAQTPLNNDTFGSCGRLISRTKAKVIDLEDGVTTLGPNQQGELYLAGPQIMKGYYNEPEATKEMISEDGWLRTGDVAYYDEEGNFYIVDRLKELIKVKGFQVAPAELEDILGTHPAIAEAAVIGIPDEHAGELPRAYVVKKAGMESVSDAEIRSFVDTKVSSHKQIKGGVQFCDALPKNNMGKVLRRELRAQ 589