voltage-dependent p q type calcium channel, maker-scaffold308_size214241-snap-gene-1.44 (gene) Tigriopus kingsejongensis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000005510 (supercontig:LSalAtl2s:LSalAtl2s297:203090:236595:1 gene:EMLSAG00000005510 transcript:EMLSAT00000005510 description:"maker-LSalAtl2s297-augustus-gene-2.17") HSP 1 Score: 820.846 bits (2119), Expect = 0.000e+0 Identity = 470/593 (79.26%), Postives = 500/593 (84.32%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDK--AEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTDDIDKNQV 594
L YAE LFLGAFVFEM IRLYALGP YFSSAFNRFDC VITGS+FEV+WVNL+PRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF D TPTSNFNT INALLTV LTEEDWN+VMY AI+SQGGR GG++YS+YFVMLTL GNYCLLNVFLAIACDSLDQAAELTA EEAEKER + E E L A+G L GD EEE GG D +++RPILPYSSLFIL+STNP RVG+HW VTKP FDGFIM VILLSSA+LAAEDPV+E+ PRN +L FDY FTAIF IEC LK+LDLG HPGS+ RD+WN++D VV CALIS YFQGTP GQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKF YC+DA+K AEECQGEYF GE G+ P V R W IKAFNYDNCGAA+ITLFAVQTSEGWVDVLQNSMSSTFEDEGP PWF TEMAIFYIVYFVVFPFFFVNIFVALVIVTFN+LGEAELTDDIDKNQ+
Sbjct: 37 LYYAEFLFLGAFVFEMCIRLYALGPFIYFSSAFNRFDCVVITGSIFEVLWVNLQPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFPDETPTSNFNTIINALLTVFQVLTEEDWNNVMYXAIVSQGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSLDQAAELTAAEEAEKERQEEEQERQRLREQQELKAIGADGQL-GDELRGDEEE---------GGEPPLPDMDDEDQVRPILPYSSLFILTSTNPFRVGLHWFVTKPFFDGFIMIVILLSSASLAAEDPVEEHHPRNLLLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALISFYFQGTPMGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNSMSSTFEDEGPFPWFSTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNELGEAELTDDIDKNQL 619
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000008334 (supercontig:LSalAtl2s:LSalAtl2s508:27226:174463:1 gene:EMLSAG00000008334 transcript:EMLSAT00000008334 description:"maker-LSalAtl2s508-snap-gene-1.26") HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0 Identity = 392/598 (65.55%), Postives = 458/598 (76.59%), Query Frame = 0
Query: 8 VYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEE--LRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTDDIDKNQVGPVE 598
+YAE +FLG F+ E+LIRLYALGP YFS+ FN FDC VI GS+ EV+WV+ K + GSFGLS LRALRLLRVFKVTKYWS LRNLVIALMNA+SSI+SLLFLLFLFIFIFALLGMQ+FGGSFNF GTPTSNFNT +ALLT VLT EDWN VMYTAI S+GGRKGGG+VYSLYFVMLTLCG+Y LLNVFLAIACDSLDQAA LT EEAEKER + + D E D E + A D E ++PILPYSS FILSS+NP+RV IHWL TK +FD FIM VI+LSS ALA+EDPV EN+ RN++L DYGFT IF +EC LK++DLG + HPG++ RD WN +D TVV CALIS Y GTPTGQKLKS+KLLRVLRPLK +NRVP LKAVFDCVV SLKNVFNILIVYMLF +IFA+VGVQLFNGKF +C+D ++ A+ECQG YF E P++ ER W ++F+YDNC A+ITLFA+QT +LQ+SM+ST+E+EGP PW+R EM+IFYIV+FVVFPFFFVNIFVAL+IVTFN+LGEAEL D++DKNQ ++
Sbjct: 378 LYAEFIFLGCFILEILIRLYALGPRTYFSTTFNVFDCFVIVGSIVEVIWVHFKDKGGSFGLSALRALRLLRVFKVTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFSTGTPTSNFNTITSALLTVFQVLTGEDWNQVMYTAIDSKGGRKGGGMVYSLYFVMLTLCGDYTLLNVFLAIACDSLDQAAALTEAEEAEKERHKTTLSDDIFLDMEVHDDKENKKYFDPIKAAEIAFEKAAEAAAKAATQEITIDQEENISIKPILPYSSFFILSSSNPIRVSIHWLXTKKVFDCFIMCVIVLSSIALASEDPVDENSIRNKLLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLDITVVSCALISFYHTLAGTPTGQKLKSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNVFNILIVYMLFQIIFAIVGVQLFNGKFFFCSDESRTNADECQGYYFHY-EVPNETPKIVERTWERRSFHYDNCFRAIITLFAIQT------ILQDSMASTYENEGPKPWYRVEMSIFYIVFFVVFPFFFVNIFVALIIVTFNELGEAELQDNMDKNQKSCID 968 HSP 2 Score: 76.2554 bits (186), Expect = 1.352e-14 Identity = 52/170 (30.59%), Postives = 79/170 (46.47%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISL-----------------YFQGTP--------------TGQKLKSVKLLRVLRPLKMVNRVP 421
SSLF LS NP+R + W V +F+ I+F I+ + +A + P ++ + + L + + F IF +E K++ G + H GS+ R+ WNIMD VV I + YF T TG L +++ RVLRPLK+V+ VP
Sbjct: 56 SSLFFLSGKNPIRRAVIWFVRWIVFEWIIIFTIIANCIVMALDQHLPHKDKSKLSVQLEELEPVFMGIFTMEMCFKIVADGFILHHGSYLRNPWNIMDFIVVISGSIYVHPNKSFIHISHYNDVIYYFYSTSFLPYLIPKSDKKENTGPDLSTLRTFRVLRPLKLVSGVP 225
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000004952 (supercontig:LSalAtl2s:LSalAtl2s260:1045447:1062229:1 gene:EMLSAG00000004952 transcript:EMLSAT00000004952 description:"maker-LSalAtl2s260-snap-gene-10.24") HSP 1 Score: 336.265 bits (861), Expect = 1.647e-101 Identity = 235/610 (38.52%), Postives = 321/610 (52.62%), Query Frame = 0
Query: 34 YFSSAFNRFDCAVITGSLFEVMWV--NLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGG-GLVYSLYFVMLTLCGNYCLLNVFLAIACDSL--------------------------DQAAELTANEEAEKERA----VADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGA-------------FGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTG-QKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEY--FSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
YF S FNRFDC V+ S+ E++ ++ P G+S LR +RLLR FKVT+YW+ L+NL+ +L+N++ +I SL+ LLFLF+ I+ALLG QLFGG F +LT EDWN+VMY I++ GG + G+ SLYF+ L + GNY LLNVFLAIA D+L D + + ER + D + D+ LN + D Q+ + G F ED + PI P+SSLFI S TN RV + F FI+ I++SSA+LAAEDP+ +PRN V+ G + H G++ R NI+D VV ++IS+ F+ + LK +++LRVLRPL+ VNR LK V V+ S+ + NI++V +L +FA++GVQLF GKF C+D++K T EECQG+Y F +W Q P V EREW + F+YDN M+TLF V T EGW +L S+ S ED GP+P R M IFYIVY ++ FF VNIFV VIVTF GE+ + ++DKNQ
Sbjct: 405 YFISLFNRFDCFVVLSSILELVLTRTHIMP---PLGMSVLRCIRLLRAFKVTRYWTSLKNLLKSLINSIEAIFSLIVLLFLFLGIYALLGSQLFGGKF-----------------XERILTGEDWNAVMYDGILAYGGPQTVLGMFASLYFITLFIIGNYILLNVFLAIAVDNLAGDDDDEEXETPVVKDPEPAFAPAQQDPSRPPPPGTQIPHEREDLLMMIDNHHLYNYDPGYEDEYNPYLNSNGY--DHYNDYNQMVNNNGPIKSLEEKLEEEKANEEAFLEDDDNKKDPIPPFSSLFIFSPTNRFRVLCYNFCNHAYFANFILVCIMVSSASLAAEDPLHSKSPRN--------------------IVIAYGAVGHKGAYARYPANILDLVVVSVSIISIAFRDSAGAISVLKILRVLRVLRPLRAVNRAKGLKLVIXAVIVSVSTIQNIVLVTVLLQFLFAVIGVQLFKGKFYSCSDSSKMTEEECQGQYVDFKKSDWAQ--PTVLEREWSLNDFHYDNVAKGMLTLFVVSTFEGWPGILYTSLDSHAEDTGPLPISRPSMFIFYIVYIIIIAFFMVNIFVGFVIVTFQKEGESPFANCELDKNQ 970 HSP 2 Score: 98.5969 bits (244), Expect = 1.678e-21 Identity = 68/251 (27.09%), Postives = 128/251 (51.00%), Query Frame = 0
Query: 286 SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQ--ENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQG-TPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNG-KFLYCTDA-----AKRTAEECQGEY-FSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWV 526
S+++ S NP+R+ +V F+ FI+ IL + +LA P + N L K + F IF EC +K++ GL H ++ R+ WN +D ++V + + + G +K+++ RVLRPL++V+ VP+L+ V + ++ ++ +F+I ++ + ++I++++G+Q+F G C D C G Y +G ++IP N+DN +M+T+F T EG++
Sbjct: 47 SIYLFSLKNPIRLKCITVVEWKAFEFFILATILATCVSLALHKPYPNGDTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGFMDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMFQGLMHKACFDNITGVLIDDDPRLCGGNYQCETGVCREYIPGPN-----FGITNFDNFILSMLTVFQCITLEGFM 292 HSP 3 Score: 74.7146 bits (182), Expect = 3.832e-14 Identity = 57/222 (25.68%), Postives = 106/222 (47.75%), Query Frame = 0
Query: 8 VYAELLFLGAFVF------EMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFG-LSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGG-SFNFDDGTPTS----NFNTAINALLTVLTEEDWNSVMYTAIISQ-----------GGRKG-GGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLD 205
+Y++ L L ++F E +I++ A P YF +N FDC ++ GS ++ +L G ++ R R LR+ K+ +R L+ + + ++ ++ L+ L FI+ ++GMQ+FG + N D ++ NF +A+ L T E W +M + I S G G G ++ YF+ + ++ +LN+F+A+ D+ D
Sbjct: 1030 IYSDFLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINEDTSIHSNNNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDNFD 1251 HSP 4 Score: 56.6102 bits (135), Expect = 1.905e-8 Identity = 65/302 (21.52%), Postives = 125/302 (41.39%), Query Frame = 0
Query: 293 TNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSV----KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSS--------TFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDL 580
++PV+ I F+ + ILL++ +LA N P+ + L +Y FT F +E +K+ F P +F D WN D +V + I + +G +L R LR +K++++ +++ + + S + + ++++ L I+ +VG+Q+F GK D + + N+ N +A+I LF T E W D++ + + S + E P ++I +F+ F +N+FVA+++ F+ L
Sbjct: 989 SDPVQYRIWAWAXSSKFEYVVFGAILLNTISLAMS---FYNQPQIYSDFLDLLNYIFTIFFALEFVIKMA----AFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVF-GKIAINEDTSIHSNN-----------------------------NFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDNFDYL 1253
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000010843 (supercontig:LSalAtl2s:LSalAtl2s721:13130:108134:1 gene:EMLSAG00000010843 transcript:EMLSAT00000010843 description:"maker-LSalAtl2s721-augustus-gene-1.27") HSP 1 Score: 324.324 bits (830), Expect = 5.211e-97 Identity = 222/551 (40.29%), Postives = 312/551 (56.62%), Query Frame = 0
Query: 84 KYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF-----NFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEG--------------GGGGAFGEDGLEELR-----------PILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEY--FSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQVGPVE 598
+YW L+NL+ +L+N++ +I+SLL LLFLF+ IFALLG Q+FGG + + + +NF++ +LL+V LT EDWN VMY I + GG G+V SLYF++L +CGNY LLNVFLAIA D+L A L +EAEKE E E + A GD R + + D + G F D LR PI S+ +I N R+ H++ F ++ I++SSA LAAEDP+ N+ RN++L+ FDY FT IF IE LKV+ G + HPG+F R +N++D VV +LIS F +K +++LRVLRPL+ +NR LK V CV+ + K + NI++V +L +FA++GVQLF GKF C+D +K TAE C+G++ + G+ Q P V ER W F+YDN G ++TLF V T EGW +L S+ S ED GPI +R +A+FY +Y ++ FF VNIFV VIVTF + GE+ + ++DKNQ +E
Sbjct: 783 RYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAFGGVNSIGIVSSLYFIILFICGNYILLNVFLAIAVDNLADADAL--EDEAEKEEGAGPPEDG-GVEGVDILGANSHCGGDTELRLSLDQEYSLEDYDQYAEDDEIVGDDDDEEDEGQFELDDKNTLRMDSESPFYKVVPIPQESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFSSGAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCSDKSKMTAETCRGKFIVYKDGDLNQ--PIVEERIWERNDFHYDNVGKGLLTLFVVSTFEGWPGILYVSIDSNAEDVGPIHNYRPIVAVFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIE 1327 HSP 2 Score: 130.568 bits (327), Expect = 9.815e-32 Identity = 72/145 (49.66%), Postives = 97/145 (66.90%), Query Frame = 0
Query: 9 YAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVN---LKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV 150
+ L F+ F FEM +++Y+LG YF S FNRFDC V+ S+ E++ N + P GLS LR +RLLR FKVT+YWS + NLV +L+N+++SI SLL +LFLFIFIF+LLGMQ+FGG F S+F+T + LTV
Sbjct: 635 FTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTNENIMPP----LGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFT-KSLESRSHFDTFPQSCLTV 774 HSP 3 Score: 86.2705 bits (212), Expect = 1.181e-17 Identity = 83/317 (26.18%), Postives = 136/317 (42.90%), Query Frame = 0
Query: 286 SLFILSSTNPVRVG-IHWLVTKPLFDGFIMFVILLSSAALAAEDP--VQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTP-TGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGE-AELTDDIDK 591
S++ + NP+R I ++ KP F+ I+ IL + ALA P ++ N++L K +Y F IF EC +K++ G HP ++ R WN++D T+V +IS G +K+++ RVLRPL++V+ VP + + TD C G Y E G V W F N+D+ G +M+T+F T EGW DVL Q++M S+++ I+++ V+ FF +N+ + ++ F+ E A+ D K
Sbjct: 151 SIYCWTIKNPIRKRFIQFVEWKP-FEFLILLTILGNCVALAIYTPFPAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEGFDVKALRAFRVLRPLRLVSGVP----------------------------------------REMEETD----NPTLCGGNYKCDEEAGY----VCREYWEGPNFGITNFDHFGLSMLTVFQCITLEGWTDVLYWVQDAMGSSWQ------------FIYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQK 406 HSP 4 Score: 67.781 bits (164), Expect = 6.877e-12 Identity = 53/212 (25.00%), Postives = 99/212 (46.70%), Query Frame = 0
Query: 12 LLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGT---PTSNFNTAINALLTVL---TEEDWNSVM-----YTAII------SQGGRKGGGLVYSL-YFVMLTLCGNYCLLNVFLAIACDSLD 205
++F F E + +L A YF +N FD ++ GSL ++ L P ++ R R++R+ K+ +R L+ + ++ + L+ L FI+ ++GMQ+F G +G+ +NF T + ALL + T E W +M + +I +G G G ++ YF+ + ++ +LN+F+A+ D+ D
Sbjct: 1392 VIFTVFFTLEFVFKLGAFRFKNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGSNINRNNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 1602 HSP 5 Score: 60.8474 bits (146), Expect = 8.399e-10 Identity = 70/298 (23.49%), Postives = 130/298 (43.62%), Query Frame = 0
Query: 294 NPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSV---KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSST-------FEDEGPIPWFRTEMAI-FYIVYFVVFPFFFVNIFVALVIVTFNDL 580
NP++ + VT P+F+ I +IL+++ +LA + Q+ L + FT F +E K LG F ++F D WN D +V +LI + G + S+ +L RV+R +K++++ ++ + S + + + I+ L I+ +VG+Q+F G+ P+ E + + N+ A++ LF T E W +++ + ED G T+ A ++I +FVV F +N+FVA+++ F+ L
Sbjct: 1342 NPIQYKLWAFVTSPVFEYTIFAMILINTLSLAMKF-YQQPQTYTAFLDVLNVIFTVFFTLEFVFK---LG-AFRFKNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF----------------------------GKIAPQ--EGSNINRNNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYL 1604 HSP 6 Score: 58.9214 bits (141), Expect = 3.807e-9 Identity = 86/422 (20.38%), Postives = 170/422 (40.28%), Query Frame = 0
Query: 241 GGLNGDAAA-RKEEEDRR-----QVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFI-----ECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQG----TPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR---TAEEC----QGEYFSSGEW----------------------------------------GQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE-LTDDIDKNQ-VGPVE 598
G N D++ R +E R ++ + G GGA DG E + R +V I+ ++ L++ LA E Q L F FT +FF+ E FLK+ LG +F ++N D VV +++ L P G L ++ +R+LR K+ ++ + +V S+ ++ ++L++ LF+ IF+++G+Q+F GKF ++ + C QG S W G+++ + + N+D+ +++++F + T E W V+ + + + G + ++++I+ F+ + +N+F+A+ + ++L +A+ L D+ +K + GP E
Sbjct: 529 GAKNKDSSTNRSSKEISRDSFSARLTNEADGKGGALDVDGTGEPSSWFQKKKKALEKLNRRARRVCRKIVKSXAMFWIIIILVFLNTCVLATEHYKQP-----IWLDDFQE-FTNLFFVVLFTFEMFLKMYSLGFQ----GYFVSLFNRFDCFVVISSILELILTNENIMPPLG--LSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFTKSLESRSHFDTFPQSCLTVFQGFEDSERYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAF----GGVNSIGIVSSLYFIILFICGNYILLNVFLAIAV---DNLADADALEDEAEKEEGAGPPE 931 HSP 7 Score: 52.373 bits (124), Expect = 3.529e-7 Identity = 36/128 (28.12%), Postives = 65/128 (50.78%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSG-LRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF 129
L Y + F F E+ +++ + G P A+ S FN D V+ SL ++ +G+ + LR LR+LR + L+++V ++ A +I +++ + L F+FA++G+QLF G F
Sbjct: 1069 LNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIF-----SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKF 1191
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000003972 (supercontig:LSalAtl2s:LSalAtl2s2133:21116:38912:-1 gene:EMLSAG00000003972 transcript:EMLSAT00000003972 description:"maker-LSalAtl2s2133-snap-gene-0.4") HSP 1 Score: 257.684 bits (657), Expect = 6.346e-80 Identity = 131/222 (59.01%), Postives = 165/222 (74.32%), Query Frame = 0
Query: 379 MDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQV 594
MD VV CA+ S G+ G+ L KS+++LRVLRPLK + RVP LKAVFDCV+ SLKNVFNIL+VY+LF IFA++ VQLFNGKF YC+DA+K+ AEE GEYF P+V R W + F+YDN A++TLFAVQT+EGW +LQ SM++TFED+ PIP+FR M+IFYIVYF+VFPFFFVNIFVAL+I+TF + GE+EL + +IDK V
Sbjct: 1 MDMLVVSCAITSFAMDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAEE--GEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKKSV 220
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000006853 (supercontig:LSalAtl2s:LSalAtl2s3888:1494:5853:1 gene:EMLSAG00000006853 transcript:EMLSAT00000006853 description:"maker-LSalAtl2s3888-augustus-gene-0.2") HSP 1 Score: 231.106 bits (588), Expect = 4.808e-72 Identity = 133/194 (68.56%), Postives = 158/194 (81.44%), Query Frame = 0
Query: 22 MLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTA 212
M +R++ALGP Y+ S+FNRFDC VI+GS+FEV+W + KP+AGSFG S LRALRLLR+FKVT YWS LRNLVI+L++++ SI+SLL LLFLFI IFALLGMQLFGG+FNF DGTP SNFN+ A+LTV LT EDWN VMY I SQGG + GL+YSLYF+ LT+ GNY LLNVFLAIA DSL QA E+TA
Sbjct: 1 MFLRMWALGPRIYYESSFNRFDCLVISGSVFEVVWTHFKPKAGSFGFSVLRALRLLRIFKVTAYWSSLRNLVISLLSSMKSIISLLLLLFLFILIFALLGMQLFGGTFNFPDGTPPSNFNSFSIAMLTVFQILTGEDWNEVMYQGIESQGGAQ-AGLIYSLYFIFLTMFGNYTLLNVFLAIAVDSLGQAQEMTA 193
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000009889 (supercontig:LSalAtl2s:LSalAtl2s643:800:51309:-1 gene:EMLSAG00000009889 transcript:EMLSAT00000009889 description:"maker-LSalAtl2s643-snap-gene-0.8") HSP 1 Score: 185.267 bits (469), Expect = 2.262e-50 Identity = 99/164 (60.37%), Postives = 124/164 (75.61%), Query Frame = 0
Query: 289 ILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLF 447
++++ +R+ +H +VT P FD FIM +I++SS ALAAEDPVQEN+ RN +L+ FDY FT IF +E LKV+DLGL+ HPGS+ RDVWN+MD VV CA IS F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF
Sbjct: 601 LVANAGQIRLFVHGIVTFPFFDTFIMIIIIMSSIALAAEDPVQENSRRNHILTYFDYVFTGIFAVEMILKVIDLGLIAHPGSYLRDVWNLMDALVVICASISFIFDMTGSSKGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVTNILIVYILF 764 HSP 2 Score: 130.183 bits (326), Expect = 7.971e-32 Identity = 71/109 (65.14%), Postives = 82/109 (75.23%), Query Frame = 0
Query: 121 GMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANE-EAEKERAVADA 225
GMQLFGG+FNF DGTP SNFN+ +LLTV LT EDWN VMY I SQGG + G+VYSLYF++LTL GNY LLNVFLAIA D+L A ELTA E +AE + VA+A
Sbjct: 498 GMQLFGGAFNFSDGTPPSNFNSFAISLLTVFQILTGEDWNEVMYQGIESQGGHR-SGMVYSLYFIILTLFGNYTLLNVFLAIAVDNLANAQELTAAENQAENDELVANA 605 HSP 3 Score: 85.8853 bits (211), Expect = 1.023e-17 Identity = 69/243 (28.40%), Postives = 113/243 (46.50%), Query Frame = 0
Query: 318 LLSSAALAAEDPVQENAPRN---RMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD------GTVVFCALISLYFQGT-----------------PTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKF-----------LYCTDAAKRTAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITL 516
LL++ + A + N R + L K + F IF +E LK+L LG + H GS+ R+ WNIMD G V F A S+ F T G L++++ +RVLRPLK+V+ VP+L+ V ++ +L + I ++ M +LIFA++G++ ++G L TD + + C + + G H E + W + ++DN G AM+T+
Sbjct: 22 LLTNCIVLALEEHLPNGDRTIFAQKLEKTEPYFLGIFTVEAALKILALGFVLHRGSYLRNAWNIMDFIVVVTGMVTFGAEASIPFDLTIVRSFRESEASSSSIPSEEGVDLRTLRAIRVLRPLKLVSGVPSLQVVLKSILKALAPLMQIALLVMFAILIFAIIGLEFYSGALHKACYSVENLELMVTDGSLKVP--CHSDDPLTAPAGAHTCEPNVSICLENWEGPNYGITSFDNIGFAMLTV 262
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000009714 (supercontig:LSalAtl2s:LSalAtl2s627:140867:158129:1 gene:EMLSAG00000009714 transcript:EMLSAT00000009714 description:"maker-LSalAtl2s627-snap-gene-1.23") HSP 1 Score: 167.162 bits (422), Expect = 9.973e-44 Identity = 164/640 (25.62%), Postives = 280/640 (43.75%), Query Frame = 0
Query: 14 FLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGT-------PTSNFNTAINALLTVL-------TEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVF------------------------LAIACDSLDQAAELTANEEAEK-----ERAVADAEAALQAEHAALD----KAEGGLNGDAAARKEEE-----DRR------QVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPV-------RVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAP-RNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLY--FQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEV------AEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFND 579
F F FE ++L A+ P +F+ +N FD ++ SL E++ G GLS LR+ RLLRVFK+ K W L +++ + N L ++ +L +L + IFIFA++GMQLFG SF + P NF +++ + V E W + MY A G YF+ L N +LN+F ++IA + + T + E+ R +A +++ A + K G + G E + D++ Q D E +E+ P Y + + +P R+ L+ F+G ++ +ILLSS + ED E P L D T +FF+E LK+ +G P +F + W +D +V +LI+ G K+++ LR LRPL+ ++++ +K V + ++ +L ++FN+L+V ++F LIFA++GV LF G F C D+ G FS + IP +W+ +DN A + L V T +GW+ ++ +++ S ++ P M +++ + ++ FF +N+ V ++I FN+
Sbjct: 671 FTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILSLVELL------AXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFG-SFYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGEWIESMW-ACMYVA----------GPTCVPYFLATVLVANLVILNLFLALLLSSFSDMGGSGEEEDGKPDKMSIAVNRFKRLFSFTKRKIKEELFDRIXRKIAHSKSKPLIPEANGNGFSHKTNGYIGGHNNVPSETQTYIPIDQQSDKFAPQTKDHEEIYYDPLETLEVEDCCPPFVYRIIPLGDQHSPFWEAWTQQRLMAMRLIENEYFEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMG----PVMYFTNAWCWLDFVIVAXSLINFIASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDS-------IXGHKFSH----EIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNE 1277 HSP 2 Score: 69.707 bits (169), Expect = 1.378e-12 Identity = 76/335 (22.69%), Postives = 141/335 (42.09%), Query Frame = 0
Query: 286 SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFN------------------------------------------YDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFN 578
+LF L+ +P+R ++T PLF+ I+ IL++ + P S + FT+I+ E +K+L G + H ++ RD WN +D V+ + I++ G S++ RV R LK V VP LK + ++ S+KN+F++ I+ + L +FA++G+QL+ G +R E E H + W I + N +DN G A + F + T + W ++ Q ++ + PW +F+I+ + F+ +N+ +A+V ++++
Sbjct: 146 ALFFLTPFHPIRRIAIRVLTHPLFNFIIICTILVNCIVMML--------PDTEYXSASEIIFTSIYTYESAVKLLARGFILHNFTYLRDPWNWLDFAVIGMSYITI---AIDLGS-FSSLRTFRVFRALKSVAXVPGLKTIVGAIIYSVKNLFDVTILTLFGLALFALLGLQLYMGVL------TQRCIYEIPDELLEMNNGSVH---SVYKSWYINSSNLYVSEVTNDYIMCGNSSGAGKCPEGTVCLAGFAQNPNHGYTTFDNFGYAYLCAFRLMTQDYWENLYQITLRTAG------PW----NILFFILNIFMGSFYLINLILAIVAMSYD 449 HSP 3 Score: 68.5514 bits (166), Expect = 3.889e-12 Identity = 104/489 (21.27%), Postives = 181/489 (37.01%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN------------------------------FDDGTPTSNFNTAINALLTVLTEEDWNSVMYTAI----ISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNG 461
L Y + + F E ++L+A+GP YF++A+ D ++ SL + +L +R LR LR + ++ +V AL+ AL SI ++L + +F IFA++G+ LF G+F+ + N A ALL V T + W ++M AI I++ + + YFV + G++ LN+ + + D ++ K +GG + DA ED+++ A L RP ++ + +VT FD IM I L+ + D Q+ +L + F IF +E LK+ L +F + WN+ D +V +L L+ R ++ + +V +L + NI ++ L + IFA+ G+ LF
Sbjct: 1045 LYYLDRILTVVFFLETTLKLFAMGPVMYFTNAWCWLDFVIVAXSLINFI-ASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDSIXGHKFSHEIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYREASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNE------------------------------QKNKGGSSLDAFM---TEDQKKYIAAMKKASKKKPLKALP--RPYWKPQAI------------VFGIVTNKKFDMIIMGFIGLNMLTMMM-DHYQQTEGWTFVLDNLNVFFIMIFTLEMLLKIFALR-----QYYFSEPWNLFDFFIVLLSLGGLFL-------------------------RAKGIRTLLFSLVMALPALGNICLLLFLVMFIFAVFGMSLFKN 1454 HSP 4 Score: 60.077 bits (144), Expect = 1.586e-9 Identity = 43/136 (31.62%), Postives = 69/136 (50.74%), Query Frame = 0
Query: 337 NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR 472
N +L +Y FT IF E F+K++ + P FF D WN D +V +L+ L G L+S +LLRV K+ +L + + +L + N+ +V + + IFA++G+QLF G F Y A ++
Sbjct: 661 NNLLKNGNYFFTTIFAFESFMKLMAMS----PRYFFADGWNCFDFLIVILSLVELLAXGVNGLSMLRSFRLLRV---FKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLF-GSF-YIEKACEK 787
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000007884 (supercontig:LSalAtl2s:LSalAtl2s464:432083:573266:-1 gene:EMLSAG00000007884 transcript:EMLSAT00000007884 description:"maker-LSalAtl2s464-augustus-gene-6.21") HSP 1 Score: 153.295 bits (386), Expect = 4.435e-39 Identity = 84/301 (27.91%), Postives = 165/301 (54.82%), Query Frame = 0
Query: 286 SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDP-VQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALIS-----LYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAER--EWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFN 578
+L++ + +R+ + ++ FD ++ I + LA E P + +P ++L+ + FT IF IE +K + GL++ ++F++ WNIMDG++V + + L + + L+ +L+R LRPL+++NR P LK V +++SLK + NI+++ F +IF ++GVQLF G F Y C+G + + + E++++ W+ +N+DN G A+++LF + + +GWV+++ + + D+ P F I++I + ++ FF +N+FV +V+ F+
Sbjct: 1269 TLYVFHPNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYY-----------CEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFH 1558 HSP 2 Score: 103.99 bits (258), Expect = 3.037e-23 Identity = 73/222 (32.88%), Postives = 113/222 (50.90%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVM--WVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTP----TSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSL---DQAAELTANEEAEKE 219
+ L+F F EM++++ A G Y S FN FD ++ S E+ ++ G+ GLS LR RLLR+ K+ ++ LR ++ ++ + ++ LL LFIFIF++LGM LFG F G NF++ + A +TV LT+EDWN V+++ G + +LYFV L GNY L N+ +AI + D+ N E K+
Sbjct: 832 SNLVFSAIFAVEMILKVSAYGIFRYISDGFNVFDGLIVILSSIEIYNGLIHGIEMDGNSGLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKFCDKVGNQRKCDRKNFDSLLWATVTVFQILTQEDWNIVLFS------GMQRTSHWAALYFVTLMTFGNYVLFNLLVAILVEGFSNQDEKRSSDRNSEYSKD 1047 HSP 3 Score: 72.4034 bits (176), Expect = 2.195e-13 Identity = 89/369 (24.12%), Postives = 153/369 (41.46%), Query Frame = 0
Query: 247 AAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILP--YSS-----LFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTG------QKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVF-PFFFVNIFVALVIVTFNDLGEAELTD---DIDKNQVGPVE 598
A A E +R + G G ELR + P Y+S LF + + +V +F I+F IL+++ ++ E Q +++ + F+AIF +E LKV G+ + D +N+ DG +V + I +Y G G L ++ R+LR LK+V +P L+ ++ ++ NV + MLF+ IF+++G+ LF KF C + +C + F S W A +T+F + T E W VL + M T + A Y V + F + N+ VA+++ F++ E +D + K+ VG ++
Sbjct: 737 AEAIDENNKKRAIYSLNGSMTGE------HELR-VTPKKYNSIMEAFLFCSKGIRRFQAALERIVDHNIFQRGILFCILINTFSMGIEFHKQPELL-TKIVEVSNLVFSAIFAVEMILKVSAYGIF----RYISDGFNVFDGLIVILSSIEIY-NGLIHGIEMDGNSGLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKF--CDKVGNQ--RKCDRKNFDSLLW------------------------ATVTVFQILTQEDWNIVLFSGMQRT-----------SHWAALYFVTLMTFGNYVLFNLLVAILVEGFSNQDEKRSSDRNSEYSKDPVGKLD 1053 HSP 4 Score: 65.4698 bits (158), Expect = 2.855e-11 Identity = 48/179 (26.82%), Postives = 91/179 (50.84%), Query Frame = 0
Query: 286 SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNR---MLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNG 461
S L+ +NPVR ++ P F+ M VIL + L P ++ + R +L D F +E F+K++ +G+ F G + + WN +D +V + + L +++ +RVLRPL+ +NR+P+++ + ++ +L + N+L++ IF ++GVQL+ G
Sbjct: 35 SFRFLNLSNPVRKFCIRILVNPWFERLTMTVILFNCVTLGMYHPCEDTDCKTRRCQILKNCDDVIYIYFTVEMFIKMIAMGV-FGKGCYLSETWNRLDCFIVLAGALEYCLKVEKLN--LSTLRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 210 HSP 5 Score: 59.6918 bits (143), Expect = 1.762e-9 Identity = 100/450 (22.22%), Postives = 185/450 (41.11%), Query Frame = 0
Query: 13 LFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNL---------VIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTS-----------------------NFNTAINALLT--VLTEED-WNSVMYTAI----ISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAE---DPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKM 416
+F F EM ++ + G AYF + +N ++ GSL V +V+ +G + +LRVF++ + PLR + V L+++L I +++ + F IF +LG+QLF G F + +G + NF+ +AL++ VL+ +D W ++MY + + Q ++ +YF+ L + +LN+F+ + ++ + AE+E+ AA +A+ EE RR++ A PY + + R+ +H +VT F I VI L+ +A E PV L +Y FTA+F +E +K+ LG+ G + D WN +D +V +++ + + + + ++RV+R L++
Sbjct: 1330 IFTFIFTIEMAMKAISTGLIYGAEAYFKNGWN-----IMDGSLVIVSFVD--TVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRC-------RAEQEKEERARRAAKRAKKI------------------EERRRRMMKA--------------------PYYIHY-----SHYRLSVHKVVTSKYFXLAISAVIGLNVITMAMEFYMMPVVLAYA----LKVCNYFFTAVFILEAGMKISALGI----GRYLSDKWNQLDVVIVLLSVVGIVLEEIKSDMMPINPTIMRVMRVLRI 1714
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Match: EMLSAG00000010768 (supercontig:LSalAtl2s:LSalAtl2s712:648445:661651:1 gene:EMLSAG00000010768 transcript:EMLSAT00000010768 description:"maker-LSalAtl2s712-snap-gene-6.14") HSP 1 Score: 110.538 bits (275), Expect = 3.115e-25 Identity = 88/292 (30.14%), Postives = 148/292 (50.68%), Query Frame = 0
Query: 297 RVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR-NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISL--YFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPE--VAERE----WLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFND 579
R+ + L+ F+G ++ +ILLSS + ED + P ML D T +FF+E LK+ +G + +F + W +D +V +LI+ G K+++ LR LRPL+ + ++ +K V + +V +L ++FN+L+V ++F LIFA++G+ F GKF C D E GE FS + IP V E W N+DN A + LF V T +GW+ ++ +++ S ++ P M F++ + V FF +N+ V ++I FN+
Sbjct: 1175 RLQVSRLIENKYFEGVVLTLILLSSFVMTLEDIWFDTRPLLVDMLYYLDRILTVVFFLETLLKLFAMGCVM----YFGNAWCWLDFVIVAVSLINFGASLVGLGNIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKFYKCLD-------EETGEKFSH----EIIPNKTVCLNETGAVWTNARVNFDNVFIAYLALFQVATFKGWMTIMDDAIDSVEKNNQPHREININMYFFFVFFIVFGSFFTLNLLVGVIIDKFNE 1451 HSP 2 Score: 87.8113 bits (216), Expect = 4.063e-18 Identity = 52/150 (34.67%), Postives = 79/150 (52.67%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF------NFDDGTPTSNFNTAINALLTV 150
L F F E I+L A+ P +FS +N FD ++ SL E++ G GLS LR+ RLLRVFK+ K W L +++ + N ++ +L F+L + IFIFA++GMQLFG + +D P NF +++ + V
Sbjct: 788 LTQGNYFFTTIFAVESFIKLVAMSPRYFFSEGWNCFDFLIVVLSLVELL------AEGVSGLSMLRSFRLLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGKDYYDGVCEKWDCDMPRWNFTDFLHSFMIV 931 HSP 3 Score: 59.6918 bits (143), Expect = 1.792e-9 Identity = 56/203 (27.59%), Postives = 104/203 (51.23%), Query Frame = 0
Query: 12 LLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPR--AGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGG---SFNFDDGTPTSNFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAE 209
L F+ F EML++++AL YF+ +N FD V+ SL + +L + L +R ++ RV ++ K +R L+ +L+ A +++++ LLFL +FIFA+ GM LF FDD ++ + A++A +T E D S T G G+ + + +++L+ ++N+++A+ ++ QA E
Sbjct: 1518 LSFIVIFTAEMLLKMFALR-HHYFAEPWNLFDFVVVMLSLAGLFLSDLIEKYFVSPTLLRVVRVAKVGRVLRLIKGAKGIRTLLFSLVMAFPALVNICLLLFLVMFIFAVFGMSLFKNVKIRSGFDDMCTSAGWAEALDA-ITDDRECDKPS-SETGDAGNCGNYMAGVAFMIIYLILSF---LIIVNMYIAVILENYSQANE 1714 HSP 4 Score: 57.7658 bits (138), Expect = 8.058e-9 Identity = 39/127 (30.71%), Postives = 67/127 (52.76%), Query Frame = 0
Query: 339 MLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLY 465
+L++ +Y FT IF +E F+K++ + P FF + WN D +V +L+ L +G L+S +LLRV K+ +L + + + + N+ V + + IFA++G+QLF GK Y
Sbjct: 787 VLTQGNYFFTTIFAVESFIKLVAMS----PRYFFSEGWNCFDFLIVVLSLVELLAEGVSGLSMLRSFRLLRV---FKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLF-GKDYY 905 HSP 5 Score: 55.0694 bits (131), Expect = 4.598e-8 Identity = 47/216 (21.76%), Postives = 90/216 (41.67%), Query Frame = 0
Query: 409 RVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGE-----WGQHIPE------VAEREWLIKAFNY---------------------------------DNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDL 580
RV R LK V +P LK + ++ S+KN+ +++I+ M L +FA++G+Q++ G +++C Y S G WG E + W + NY DN G+A + F + T + W ++ Q ++ + PW IF++ + F+ +N+ +A+V +++++L
Sbjct: 176 RVFRALKSVAVIPGLKTIVSAIIYSVKNLRDVIILTMFALSVFALLGLQIYMGVL----------SQKCVYTYPSPGSEEFEYWGNMTAESFHAWYSNKSSWYLSNDNYIMCGNASGAGKCPDGTTCMEGFGPNPNYGYTSFDNFGSAYLCAFRLMTQDFWENLYQITLRTAG------PWH----IIFFMCNIFLGSFYLINLILAIVAMSYDEL 371 HSP 6 Score: 53.5286 bits (127), Expect = 1.653e-7 Identity = 38/130 (29.23%), Postives = 62/130 (47.69%), Query Frame = 0
Query: 4 VAELVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFG----LSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF 129
V L Y + + F E L++L+A+G YF +A+ D ++ SL +N G +R LR LR + ++ +V AL+ AL SI ++L + +F IFA++G+ F G F
Sbjct: 1216 VDMLYYLDRILTVVFFLETLLKLFAMGCVMYFGNAWCWLDFVIVAVSL-----INFGASLVGLGNIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKF 1340
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|322518442|sp|C9D7C2.1|CAC1A_APIME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Cacophony protein) HSP 1 Score: 647.506 bits (1669), Expect = 0.000e+0 Identity = 356/605 (58.84%), Postives = 439/605 (72.56%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTA----NEEAEKERAVADAEAALQAEHAALDKAEGGL-----NGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L +AE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W +K +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNFD GTP +NFNT ALLTV LT EDWN VMY I SQGG K G ++YSLYF++L L GNY LLNVFLAIA D+L A EL+A EE +K++ + E +Q+ D + NG + EE+++Q D + G +P+LPYSS+FILS TNPVR HW+V FD FIM VI LSS ALAAEDPV E++PRN +L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF YC+D +K T ++CQG+YF E G +PE +REW + F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 600 LYFAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSEVK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFDSGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKG-MIYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENEEEEEDKQKQAQEIEKEIQSLQNPKDGGAPKVEICPPNGKGGKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPVRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTQQDCQGQYFVF-EDGALLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1188 HSP 2 Score: 133.265 bits (334), Expect = 4.095e-31 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P Q+ + L + F IF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 146 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKQDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDINVIVKEGEQASPCNTDNKSEAPFGAHVCDANISTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 443 HSP 3 Score: 98.2117 bits (243), Expect = 7.060e-20 Identity = 134/626 (21.41%), Postives = 255/626 (40.73%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF-------------------NFDDGT----PTS--------NFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSM------SSTFEDEGPIPWFRTEMAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EM++++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F+DG P +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + N V+ I +V F+ FIM +I+L++ L + Q +A +N L + FT +F +EC LK+ G+ +FF+D WN D V +++ +L + + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F G D + + K N+ + ++ LF T E W +++ + + + + EG + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 930 LNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCSDESKYTQQDCQGQYFVFEDGALLPEPKKREWQSQFFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTVLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGVR----NFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF-GNIALDADTS-----------------------------ITKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCVKGRPCDAKAGKQEGGC---GSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1466
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|60391851|sp|P91645.3|CAC1A_DROME (RecName: Full=Voltage-dependent calcium channel type A subunit alpha-1; AltName: Full=Protein cacophony; AltName: Full=Protein nightblind A; AltName: Full=Protein no-on-transient B; Short=Dmca1A) HSP 1 Score: 639.417 bits (1648), Expect = 0.000e+0 Identity = 360/598 (60.20%), Postives = 438/598 (73.24%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGL--NGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF--QGTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FLG F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W +K GSFGLS LRALRLLR+FKVTKYWS LRNLVI+L+N++ SI+SLLFLLFLFI IFALLGMQLFGG FN GTP +NFNT ALLTV LT EDWN VMY IISQGG + G ++YS+YF++L L GNY LLNVFLAIA D+L A ELTA EE + E L+ E AL +A+G NGD A + ++ + + E +P+LPYSS+FILS TNP+R G HW+V P FD FIM VI +SS ALAAEDPV+EN+ RN++L+ FDY FT +F IE LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IF+++GVQLFNGKF YCTD +K T+ ECQG YF E + +P+ R W +AF+YDN AAM+TLFAVQT EGW VLQ+SM++T+ED GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 473 LYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK--GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG-MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEAL-QADGVHVENGDGAVAPSKSKGKKKEEEKKEEEEV-----TEGPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEE-DELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1060 HSP 2 Score: 122.094 bits (305), Expect = 1.486e-27 Identity = 86/316 (27.22%), Postives = 159/316 (50.32%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNG---KFLYCTDAAKRTAEE------CQGEYFSSGEWGQHIPEVAEREWLIK-------AFNYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFND 579
+SLFIL+ NP+R +++ P F+ ++ I+ + LA E+ P + + L K + F IF +E LK+L LGL+ H S+ R++WNIMD VV ++ Y Q P L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G K Y + + +E C + G + L K ++DN G AM+T+F T EGW +L +++ S F W I+++ V+ FF +N+ + ++ F++
Sbjct: 16 TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPE-VDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFN------W------IYFVPLIVIGSFFMLNLVLGVLSGEFSN 318 HSP 3 Score: 95.5153 bits (236), Expect = 4.962e-19 Identity = 130/627 (20.73%), Postives = 255/627 (40.67%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--DDGTPTS--------------------------------NFNTAINALLTVLTEEDWNSVMYTAIIS----QGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQ-GTPTGQKLKS--VKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDV----LQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIF+++G+QLF G F + D+ TS N A+ L V T E W V+ ++ + +G + + S+++++ + + +N+F+A+ +T E+ E E + D E +DK +++ D G RP+ Y + + N + + +V F+ FIM +I+ ++ L + Q + + L + GFT +F +E LK++ G+ +FF+D WNI D V +++ +L+ + G + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L + + + + N+ + ++ LF T E W ++ L+ ++ P + + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 802 LNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDK----------------NQKSCIDFTIGA------------RPLERY----MPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDM-YEKSLKYINMGFTGMFSVETVLKIIGFGV----KNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENS-----------------------------ITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1343 HSP 4 Score: 70.4774 bits (171), Expect = 3.040e-11 Identity = 141/658 (21.43%), Postives = 268/658 (40.73%), Query Frame = 0
Query: 6 ELVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDC-AVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGS-----FNFDDG----------TP----------TSNF----------------NTAIN-------ALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSL-DQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGA-----------------------FGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKF----DYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQ-KLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFF----FVNIFVALVI 574
+L E FL F E +++ ALG +Y + +N D V+TG F + + P L LRA+R+LR K+ L+ ++ +++ A++ ++ + L+ I IFA++G++ + G+ ++ +D TP T +F N+ I A+LTV +T E W +++Y G +YFV L + G++ +LN+ L + ++ + E +K R A + A+ + + +AE + A R EE++ + +A +G+DG + R S + R I V F F++ ++ L++ +A E Q + L++F ++ F +F E F+K+ LG P +F +N D V+ ++ + + G L ++ LR+LR K+ +L+ + ++ S++++ ++L + LF+LIFA++G+QLF G+F G PE N++ A++T+F + T E W +V+ +G I + + Y +YF+V F +N+F+A+ +
Sbjct: 72 KLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTG--FMTQYPQIGPEVD---LRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT-----NDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFSNERNRVERRMEFQKCRFRAMFQTAMVSYLDWITQAEEVI--LAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPS-----FLTEFLYYAEFIFLGLFMSEMFIKMYALG----PRIYFESSFNRFDCVVISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQF-----------------NLPGG-----TPET----------NFNTFPIALLTVFQILTGEDWNEVMY---------QGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAV 667
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|46576355|sp|O57483.1|CAC1S_LITCT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=FGalpha1S; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 424.476 bits (1090), Expect = 2.473e-131 Identity = 262/620 (42.26%), Postives = 365/620 (58.87%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDD-GTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAE----KERAVADAEAALQAEHAAL-----DKAEG-GLNGDAAARKEE--------EDRRQVADAEGGGGG------------AFGEDGLEELR-PILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
A + L F EM++++YALG +YF S FNRFD V+ L EVM V G+S LR +RLLR+FK+T+YW+ L NLV +L+N++ SI SLL LLFLF+ IFALLGMQ+FGG F+F+D S F+T AL+TV LT EDW +VMY I++ GG G+ +YF++L +CGNY LLNVFLAIA D+L +A LT+ ++A+ K + +A A E L KA+G G+ A + +E +D AD G E L+E P+ SS FI S TN +RV H ++ F FI+ ILLSS +LAAEDP+Q + RN++LSK D FT IF E LK+ G H GSF R+ +NI+D +VV +LIS+ + + +K +++LRVLRPL+ +NR LK V C+ ++K + NI++V L +F+ +GVQLF GKF CTD K TA+EC+G +F + + E R W F++DN + M++LF + T EGW +L ++ S ED GPI +R E+A+F+IVY ++ FF +NIFV VIVTF + GE E D ++DKNQ
Sbjct: 466 ANEVLLALFTMEMIVKIYALGFQSYFMSLFNRFDSFVVCTGLLEVMLVA-SDIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDLEVRRSTFDTFPQALITVFQILTGEDWTAVMYNGIMAYGGPTYSGMSVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLARANPDKTEEEKLLLAKKEQKAKGEGIPTTARLKIDEFESNVNEIKDPYPSADFPGDDEEEEPEIPISPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKIRVLCHRIINATTFTNFILLFILLSSISLAAEDPIQPESFRNKVLSKLDIVFTVIFTTEIVLKMTAYGAFLHKGSFCRNSFNILDLSVVGVSLISMGIESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFVAKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHAEDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVIVTFQEQGEQEYKDCELDKNQ 1083 HSP 2 Score: 119.398 bits (298), Expect = 1.214e-26 Identity = 84/267 (31.46%), Postives = 139/267 (52.06%), Query Frame = 0
Query: 286 SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF------QGTP-----TGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGK-----FLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAE-REWLIKAFN----YDNCGAAMITLFAVQTSEGWVDVL 529
+LF L NP R +V F+ I+ I + ALA P+ E+ N +L K +Y F IF IE FLK++ G + H ++ R+ WNI+D T+V + S+ QG P +G +K+++ RVLRPL++V+ VP+L+ V + ++ ++ + +I ++ + ++I+A+VG++LF+GK + TD E S+G+ Q +E R W N +DN G AM+T++ T EGW +VL
Sbjct: 30 ALFCLGLQNPFRKFCINIVEWKPFEMIILLTIFANCVALAIFLPMPEDDTNSTNSVLEKVEYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQCSINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVL 296 HSP 3 Score: 105.531 bits (262), Expect = 3.230e-22 Identity = 146/649 (22.50%), Postives = 273/649 (42.06%), Query Frame = 0
Query: 2 DSVAELVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKP--------RAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN----FDDGTPTSNFNT---------------AIN-----------------------ALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAAR---KEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSS--TNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQG----TPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE 584
+SV E V E +FL F E +++ A G AY + +N D +++ +F V+ + ++ F + LRA R+LR ++ L+ ++ +++ A+ ++ + L+ I I+A++G++LF G + F D T+ + +IN A+LTV +T E W V+Y + G +YFV L L G++ +LN+ L + + E T E K R A L+ + A + G L+ A + E R + E GG D L E+ I + + F +R LV F ++ +ILL++ +A E Q ++ + + A+F +E +K+ LG S+F ++N D VV L+ + +P G + ++ +R+LR K+ +L + ++ S++++ ++L++ LF++IFA++G+Q+F GKF + +R+ +D A+IT+F + T E W V+ N + + GP + + I++I+ FV + +N+F+A+ + ++L EAE
Sbjct: 83 NSVLEKV--EYIFLFIFTIESFLKIVAYGFILHTDAYLRNGWNILDFTIVSVGVFSVLLEQISKLQGLPAPGKSSGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMIPLLHIALLVLFMIIIYAIVGLELFSGKMHKTCYFKDTDITATVDNEKPAPCSSTGQGRQCSINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPW-----IYFVSLILLGSFFVLNLVLGVL------SGEFTKEREKAKSRG---AFQMLREQQAMDEDLRGYLDWITHAEVMDPDMEPRDGFSQLEEGGSET---DSLYEIEGINKFIAFFRQWRLWHRLLRRKSRDLVKSRFFYWLVIIIILLNTVIIATEHHHQPDS-LTKAQDIANEVLLALFTMEMIVKIYALGFQ----SYFMSLFNRFDSFVVCTGLLEVMLVASDIMSPLG--ISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDLEVRRST-------------------------------FDTFPQALITVFQILTGEDWTAVMYNGIMAY---GGPT-YSGMSVCIYFIILFVCGNYILLNVFLAIAV---DNLAEAE 667 HSP 4 Score: 93.9745 bits (232), Expect = 1.270e-18 Identity = 137/633 (21.64%), Postives = 257/633 (40.60%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF-------------------DDGTPT-----------SNFN-----TAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVY----SLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--------GTPTGQKLKSV-----KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVL----QNSMSSTFEDEGPIPWFR--TEMAIFY-IVYFVVFPFFFVNIFVALVIVTFNDL 580
+++F F E+++++ A G ++ ++FN D +V+ SL + ++ A S + LR LR+LR + L+++V L A+ +I +++ + L F+F+ +G+QLF G F DG P S+F+ + + +L T+ T E W ++Y AI S G Y +++F++ + + ++N+F+ +T E+ E+E D E LDK ++RQ RP+ Y NP + I ++VT F+ + F+I L++ +L + Q A + M + FT IF +E FLK+ F +F D WN+ D +V ++I + TP ++ + +L RVLR +K+++R ++ + + S + + + ++ ++ I+A++G+Q+F GK G HI + N+ A++ LF T E W ++L + D P + T A FY I ++++ F +N+FVA+++ F+ L
Sbjct: 834 DIVFTVIFTTEIVLKMTAYGAFLHKGSFCRNSFNILDLSVVGVSLISM---GIESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCLFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKITADECRGYFFVAKDGNPAHMEAVPRVWSHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHAEDMGPIYNYRIEIAVFFIVYIILIAFFMMNIFVGFVI--------VTFQEQGEQE--YKDCE---------LDK----------------NQRQCVQYALKA------------RPLRRYIP------KNPHQYKIWYVVTSSYFEYLMFFLITLNTISLGMQHYGQ-TAEFSYMSDILNVAFTGIFTVEMFLKLA----AFKAKGYFGDPWNVFDFLIVIGSVIDVILSEIDTPGIPATPGAEESSRISITFFRLFRVLRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIALVD--------------------GTHINRNS---------NFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPMSDFQPGEEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYL 1374
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|5921703|sp|Q25452.1|CAC1M_MUSDO (RecName: Full=Muscle calcium channel subunit alpha-1; AltName: Full=MDL-alpha-1) HSP 1 Score: 421.779 bits (1083), Expect = 2.276e-130 Identity = 260/617 (42.14%), Postives = 367/617 (59.48%), Query Frame = 0
Query: 9 YAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--DDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAE---------HAALDKAEGGLNGDAAARKEE----EDRRQVADAEGGGGGAFG-------EDGLEELRPILPY-SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQVGPVE 598
Y + F+ F EM++++Y+LG YF S FNRFDC V+ GS+ E++ + + A G+S LR +RLLRVFKVTKYW L NLV +L+N++ SI SLL LLFLFI IF LLGMQ+FGG F F ++ P SNF++ +LLTV LT EDWN VMY I + GG G+V +Y+++L +CGNY LLNVFLAIA D+L A L+ E+ ++ + D + + E H + KAE G + D + EE D + GG ++ ++ +PY +S FI S TN R+ H L F FI+ I+ SSA LAAE+P++ +A RN +L+KFDY FTA+F IE LK++ G + H G+F R +N++D VV +LIS++F +K +++LRVLRPL+ +NR LK V CV+ ++K + NI++V L +FA++GVQLF GKF C+D +K +C G Y + P + EREW F++D+ AM+TLF V T EGW +L S+ S E+ GPI FR +A +YI+Y ++ FF VNIFV VIVTF + GE E + ++DKNQ +E
Sbjct: 468 YTNIFFIALFTCEMILKMYSLGFQGYFVSLFNRFDCFVVIGSISEMVLTSSELMA-PLGVSVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEEKPRSNFDSFYQSLLTVFQILTGEDWNVVMYDGIRAYGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQIQLDNQIKNEMENEEYLQNGDHISF-KAEFGADLDTYLQDEECGSYSDDENTYNKLGGVKQRVSSLPRRNTNTDMDRIKKDIPYGTSFFIFSHTNRFRIFCHRLCNHSNFGNFILCCIMFSSAMLAAENPLKADASRNIVLNKFDYFFTAVFTIELVLKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISIFFNSNAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFSCSDGSKVYESDCHGTYLFYENGDINKPRLKEREWKNNKFHFDDVAKAMLTLFTVSTFEGWPTLLYVSIDSNKENGGPIYNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIE 1081 HSP 2 Score: 135.191 bits (339), Expect = 1.079e-31 Identity = 91/312 (29.17%), Postives = 162/312 (51.92%), Query Frame = 0
Query: 283 PYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQ--ENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQG-TPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAE---------------ECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
P +LF L+ NP+R+ +V LF+ FI+ I + ALA P ++ N+ML K +Y F IF EC +K++ G + H GS+ R+ WN +D +V +IS G +K+++ RVLRPL++V+ VP+L+ V + ++ ++ + +I ++ + ++I+A++G++LF+GK L+ T T E +C Y +W V + + N+DN G +M+T+F T EGW DVL Q++M S++E W I+++ ++ FF +N+ + ++
Sbjct: 37 PERALFCLTLKNPLRIFCIKIVDSKLFEYFILLTIFANCVALAVYTPYPSGDSNITNQMLEKIEYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTALSNLVKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGK-LHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDW------VGPNDGIT---NFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWE------W------IYFVSMVILGAFFVMNLILGVL 326 HSP 3 Score: 90.1225 bits (222), Expect = 2.020e-17 Identity = 127/639 (19.87%), Postives = 255/639 (39.91%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN---------------------------------FDDG-------TPTSNFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANE-EAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFI-----ECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGT----PTG-QKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTDDIDKNQV 594
E +FL F E ++++ A G +Y + +N D ++ + NL F + LRA R+LR ++ L+ ++ +++ A+ ++ + L+ I I+A++G++LF G + FDD T NF ++ + +T E W V+Y+ + G +YFV + + G + ++N+ L + + N + +K R E L+ + +AE A E+ + +V + E+ ++ ++ +R V F I+ ++ L++ LA E Q L +F +T IFFI E LK+ LG +F ++N D VV ++ + + P G L+ V+LLRV + K + + L A ++ S++++ ++L++ LF++IF ++G+Q+F G+F + + K + N+D+ +++T+F + T E W V+ + + + G + F I+YI+ F+ + +N+F+A+ + D + D++Q+
Sbjct: 100 EYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTALSNLVKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWEW-----IYFVSMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDPDAQIIEDCHKNKVKEVVSIDNLKDHENETQQTDSWFRSQKKYLERINRRIRRACRKAVKSQAFYWLIILLVFLNTGVLATEHYRQP-----IWLDQFQ-EYTNIFFIALFTCEMILKMYSLGFQ----GYFVSLFNRFDCFVVIGSISEMVLTSSELMAPLGVSVLRCVRLLRVFKVTKYWHSLSNLVA---SLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEEKPRS------------------------------NFDSFYQSLLTVFQILTGEDWNVVMYDGIRAY----GGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQI 684 HSP 4 Score: 65.0846 bits (157), Expect = 1.354e-9 Identity = 53/227 (23.35%), Postives = 99/227 (43.61%), Query Frame = 0
Query: 12 LLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPR---------------AGSFGLSGLRALRLLRVFKVTKYWSP---LRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTP---TSNFNTAINALLTVL---TEEDWNSVMYTAII---------SQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLD 205
++F F E + +L A YF A+N FD ++ GS ++++ +K + G L + RL RV ++ K S +R L+ + + ++ + L+ + FI+A++GMQ+F G ++GT +NF T A+L + T E W +M S G + YF+ + ++ ++N+F+A+ D+ D
Sbjct: 1146 MIFTAVFSLEFIFKLAAFRFKNYFGDAWNTFDFIIVLGSFIDIVYSEIKTKEQALATCDGQSCNKAKGGSTLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVF-GKIMLEEGTSIDRNNNFQTFPQAVLVLFRSATGEAWQEIMMACSPRDDVKCDPESDAVNNCGSSIAFPYFISFYVLCSFLIINLFVAVIMDNFD 1371
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|32172454|sp|Q24270.2|CAC1D_DROME (RecName: Full=Voltage-dependent calcium channel type D subunit alpha-1; Short=DmCa1D) HSP 1 Score: 422.165 bits (1084), Expect = 2.702e-129 Identity = 266/625 (42.56%), Postives = 361/625 (57.76%), Query Frame = 0
Query: 9 YAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVN--LKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPT--SNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHA---ALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGE------------------------DGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQVGPVE 598
Y + F+G F EML+++Y+LG YF S FNRFDC V+ GS+ E + N + P G+S LR +RLLRVFKVTKYW L NLV +L+N++ SI SLL LLFLFI IFALLGMQ+FGG FNFD NF+ ALLTV +T EDWN+VMY I + GG G + +YF++L +CGNY LLNVFLAIA D+L A L+ E EKE D E+A + H+ +D + L+ D ++E D D E E + ++ PI P +S F+ S TN RV HWL F I+ I+ SSA LAAE+P++ N N++L+KFDY FTA+F IE LK++ G + H G+F R +N++D +V C + + +K +++LRVLRPL+ +NR LK V CV+ ++K + NI++V L +FA++GVQLF GKF CTD +K T +EC G Y + H P + EREW F++D+ M+TLF V T EGW +L S+ S E+ GPI FR +A +YI+Y ++ FF VNIFV VIVTF + GE E + D+DKNQ +E
Sbjct: 1019 YTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMP---PLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLS---EVEKEEEPHD-ESAQKKSHSPTPTIDGMDDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKILPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLD-LLVVCVSLISLVSSSNAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCDLDKNQRNCIE 1635 HSP 2 Score: 134.035 bits (336), Expect = 2.249e-31 Identity = 93/317 (29.34%), Postives = 159/317 (50.16%), Query Frame = 0
Query: 279 RPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPV--QENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF-QGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLY-CTDAAKRTAEE----------CQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFN 578
+P P +LF LS NP+R +V F+ I+ I + ALA P ++ N+ L K +Y F IF EC +K+L G + H G++ R+ WN++D T+V IS Q +K+++ RVLRPL++V+ VP+L+ V + ++ ++ +F+I ++ + ++I+A++G++LF+GK C D EE C Y G W + N+DN G AM+T+F T EGW DVL Q++M S ++ W +++I ++ FF +N+ + ++ F+
Sbjct: 587 QPDRPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKACRDEITGEYEENIRPCGVGYQCPPGYKCYGGWDGPNDGIT---------NFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQ------W------MYFISMVILGAFFVMNLILGVLSGEFS 882 HSP 3 Score: 65.4698 bits (158), Expect = 1.099e-9 Identity = 55/233 (23.61%), Postives = 102/233 (43.78%), Query Frame = 0
Query: 12 LLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPR--------------------AGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDG---TPTSNFNTAINALLTVL---TEEDWNSVMYTAII-----------SQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLD 205
++F F E + +L A YF A+N FD ++ GS ++++ +K + AGS ++ R R++R+ K+ +R L+ + + ++ + L+ L FI+A++GMQ+F G D G T +NF T A+L + T E W +M + + G G + Y + LC ++ ++N+F+A+ D+ D
Sbjct: 1700 MIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVF-GKIALDGGNAITANNNFQTFQQAVLVLFRSATGEAWQEIMMSCSAQPDVKCDMNSDTPGEPCGSSIAYPYFISFYVLC-SFLIINLFVAVIMDNFD 1930
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|1209676875|sp|Q02485.2|CAC1S_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=ROB1; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 414.461 bits (1064), Expect = 3.192e-127 Identity = 264/621 (42.51%), Postives = 369/621 (59.42%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDG-TPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKE--------RAVAD-AEAALQAEHAALDKAEGGLNGDAAARKE----EEDRRQVADAEGGGG--GAFGEDGLE-----------ELR------PILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
A + L F EML+++Y LG YF S FNRFDC V+ + E++ V G+S LR +RLLR+FK+TKYW+ L NLV +L+N++ SI SLL LLFLF+ IFALLGMQLFGG ++F+D SNF+ AL++V LT EDWNSVMY I++ GG G++ +YF++L +CGNY LLNVFLAIA D+L +A LT+ ++A+ E R + D +E L++ G A+ + E + +V D G ED E EL+ PI SS FI S TN +RV H +V F FI+ ILLSSAALAAEDP++ ++ RN++L FDY FTA+F +E LK+ G H GSF R+ +NI+D VV +LIS+ + + +K +++LRVLRPL+ +NR LK V CV +++ + NI++V L +FA +GVQLF GKF C D +K T EEC+G Y+ + E+ R+W+ F++DN +AM++LF V T EGW +L ++ S ED GP+ R EMAIF+I+Y ++ FF +NIFV VIVTF + GE E + ++DKNQ
Sbjct: 467 ANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVE-SGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAIS-VVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQ 1085 HSP 2 Score: 108.612 bits (270), Expect = 3.191e-23 Identity = 86/338 (25.44%), Postives = 161/338 (47.63%), Query Frame = 0
Query: 272 EDGLEELRP------ILPYS--SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQE--NAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGT--------PTGQK-----LKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGK-----FLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNY-----DNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
++GL + +P ILP +LF L+ NP+R +V F+ I+ I + ALA P+ E N N L K +Y F +F IE +K++ G +FH ++ R WN++D +VF + + + P K +K+++ RVLRPL++V+ VP+L+ V + + ++ +F+I ++ + ++I+A++G++LF GK + TD E +G +E N+ DN G +M+T++ + EGW DVL +++ + + PW I+++ ++ FF +N+ + ++
Sbjct: 8 DEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEW------PW------IYFVTLILLGSFFILNLVLGVL 333 HSP 3 Score: 90.8929 bits (224), Expect = 1.177e-17 Identity = 131/640 (20.47%), Postives = 257/640 (40.16%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLF-------EVMWVNLKP---RAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN------------------------FDDGTPTS---------------------NFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGED--GLEELRPILPYSSLFILSSTNPV-RVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF--QGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE 584
E FL F E +++ A G AY S +N D ++ +F ++ N P + + LRA R+LR ++ L+ ++ ++ A+ + + L+ + I+A++G++LF G + G P + NF ++ + ++ E W V+Y + G +YFV L L G++ +LN+ L + + E T E K R L+ + + G ++ + D + GG+ E +E L I+ + + N V R H LV +F ++ ++ L++ ++A+E N P + + A+F IE +K+ LGL +F ++N D VV ++ + G T + ++ +R+LR K+ +L + ++ S++++ ++L++ LF++IFA++G+QLF G++ + +R+ N+DN A+I++F V T E W V+ N + + G + + I++I+ FV + +N+F+A+ + ++L EAE
Sbjct: 90 EYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPW-----IYFVTLILLGSFFILNLVLGVL------SGEFTKEREKAKSRGTFQK---LREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHW--RQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASE---HHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLR----QYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRS-------------------------------NFDNFPQALISVFQVLTGEDWNSVMYNGIMAY----GGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAV---DNLAEAE 668 HSP 4 Score: 81.6481 bits (200), Expect = 1.011e-14 Identity = 132/650 (20.31%), Postives = 251/650 (38.62%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN-------------------FDDGTPTS----------------NFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVM------LTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPT----------------------GQKLKSV--KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVL----QNSMSSTFEDEGPIPWFR--TEMAIFY-IVYFVVFPFFFVNIFVALVIVTFNDL 580
L Y + +F F E+++++ G ++ + FN D V+ SL + L+ A S + LR LR+LR + L+++V + A+ +I +++ + L F+FA +G+QLF G F + DG PT N +A+ +L TV T E W ++Y AI S + G VY+ M + + ++N+F+ +T E+ E E ++ LDK ++RQ RP+ Y NP + + ++VT F+ + +I+L++ L + ++ N + + FT IF +E LK++ F P +F D WN+ D +V ++I + T ++ S +L RV+R +K+++R ++ + + S + + + ++ ++ I+A++G+Q+F GK + D + + + N+ A++ LF T E W ++L D P + T A +Y I ++++ F +N+FVA+++ F+ L
Sbjct: 832 LEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISM---GLESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDS--NEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVI--------VTFQEQGETE-----------YKNCELDK----------------NQRQCVQYALKA------------RPLRCYIP------KNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQH-YNQSEQMNHISDILNVAFTIIFTLEMILKLI----AFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMF-GK-IAMVDGTQ----------------------------INRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYL 1387
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|209572767|sp|Q13698.4|CAC1S_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 413.69 bits (1062), Expect = 7.026e-127 Identity = 262/621 (42.19%), Postives = 367/621 (59.10%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDG-TPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEA---EKERAV--------ADAEAALQAEHAALDKAEGGLNGDAAARKEE--------EDRRQVADAEGGGGGAFGEDGL-------------EELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
A + L F EML+++Y LG YF S FNRFDC V+ + E++ V G+S LR +RLLR+FK+TKYW+ L NLV +L+N++ SI SLL LLFLFI IFALLGMQLFGG ++F+D SNF+ AL++V LT EDW S+MY I++ GG G++ +YF++L +CGNY LLNVFLAIA D+L +A LT+ ++A EK+R ++ E + A+ G+ A + +E +D AD G E L E+ PI SS FI S TN +RV H +V F FI+ ILLSSAALAAEDP++ ++ RN++L FD GFT++F +E LK+ G H GSF R+ +N++D VV +LIS+ + + +K +++LRVLRPL+ +NR LK V C+ ++ + NI++V L +FA +GVQLF GKF CTD +K T EEC+G Y+ + E+ REW+ F++DN +AM++LF V T EGW +L ++ S ED GPI R EMAIF+I+Y ++ FF +NIFV VIVTF + GE E + ++DKNQ
Sbjct: 467 ANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVE-SGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAIS-VVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQ 1085 HSP 2 Score: 114.39 bits (285), Expect = 4.719e-25 Identity = 88/338 (26.04%), Postives = 164/338 (48.52%), Query Frame = 0
Query: 272 EDGLEELRP------ILPYS--SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQE--NAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGT--------PTGQK-----LKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGK-----FLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNY-----DNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
++GL + +P ILP +LF L+ NP+R +V F+ I+ I + ALA P+ E N N L K +Y F +F IE +K++ G +FH ++ R WN++D T+VF + ++ + P K +K+++ RVLRPL++V+ VP+L+ V + + ++ +F+I ++ + ++I+A++G++LF GK + TD E +G + +E N+ DN G +M+T++ T EGW DVL +++ + + PW I+++ ++ FF +N+ + ++
Sbjct: 8 DEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEW------PW------IYFVTLILLGSFFILNLVLGVL 333 HSP 3 Score: 88.9669 bits (219), Expect = 4.558e-17 Identity = 132/649 (20.34%), Postives = 264/649 (40.68%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVM--WVN--------LKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF---------------DDGTPTS------------------------------NFNTAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQA--EHAALDKAEGG-----LNGDAAARKEEEDRRQVADAEGGGGGAFGE-DGLEEL----RPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF--QGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE 584
E FL F E +++ A G AY S +N D ++ +F V+ VN + + + LRA R+LR ++ L+ ++ ++ A+ + + L+ + I+A++G++LF G + ++ +P + NF ++ + +T E W V+Y + G +YFV L L G++ +LN+ L + + E T KER A + Q E LD+ G G+ ++ + + D G + E GL ++ R ++ +F R H +V +F ++ ++ L++ ++A+E N P R+ + ++F E +K+ LGL +F ++N D VV ++ + G T + ++ +R+LR K+ +L + ++ S++++ ++L++ LF++IFA++G+QLF G++ + +R+ N+DN A+I++F V T E W ++ N + + G + + I++I+ FV + +N+F+A+ + ++L EAE
Sbjct: 90 EYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPW-----IYFVTLILLGSFFILNLVLGVL------SGEFT------KEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIF--------RWKCHDIVKSKVFYWLVILIVALNTLSIASE---HHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLR----QYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRS-------------------------------NFDNFPQALISVFQVLTGEDWTSMMYNGIMAY----GGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAV---DNLAEAE 668 HSP 4 Score: 73.1738 bits (178), Expect = 4.165e-12 Identity = 129/650 (19.85%), Postives = 248/650 (38.15%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF-------------------NFDDGTP-----------TSNFN-----TAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVM------LTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPT----------------------GQKLKSV--KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVL----QNSMSSTFEDEGPIPWFR--TEMAIFY-IVYFVVFPFFFVNIFVALVIVTFNDL 580
L + ++ F F E+++++ G ++ + FN D V+ SL + L+ A S + LR LR+LR + L+++V + A+S+I +++ + L F+FA +G+QLF G F + DG P S+F+ +A+ +L TV T E W ++Y AI S + G +Y+ M + + ++N+F+ +T E+ E E ++ LDK ++RQ A L P PY + + ++VT F+ + +I+L++ L + ++ N + + FT IF +E LK+ + F +F D WN+ D +V ++I + T ++ S +L RV+R +K+++R ++ + + S + + + ++ ++ I+A++G+Q+F L R N+ A++ LF T E W ++L + D P + T A +Y I ++++ F +N+FVA+++ F+ L
Sbjct: 832 LKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISM---GLESSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNA--EDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVI--------VTFQEQGETE-----------YKNCELDK----------------NQRQCVQY------ALKARPLRCYIPKNPY------------QYQVWYIVTSSYFEYLMFALIMLNTICLGMQH-YNQSEQMNHISDILNVAFTIIFTLEMILKL----MAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNN------------------------------NFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYL 1387
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|6165986|sp|Q99244.2|CAC1D_MESAU (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=Calcium channel, L type, alpha-1 polypeptide isoform 2; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 400.979 bits (1029), Expect = 3.953e-123 Identity = 266/621 (42.83%), Postives = 367/621 (59.10%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDG-TPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQA-EHAALDKAEGG--LNGDAAA-----RKEEEDRRQVADAE------------------GGGGGAFGEDGLEE-LRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQVGPVE 598
A + L F EML+++Y+LG AYF S FNRFDC V+ G + E + V L+ G+S R +RLLR+FKVT++W+ L NLV +L+N++ SI SLL LLFLFI IF+LLGMQLFGG FNFD+ T S F+ ALLTV LT EDWN+VMY I++ GG G++ +YF++L +CGNY LLNVFLAIA D+L A L ++ E E A ++ E+ +K E N D ++E ED+ + G E ++E + PI S+ FILS TNP+RVG H L+ +F I+ I+LSSAALAAEDP++ ++ RN +L FDY FTAIF +E LK+ G H G+F R+ +N++D VV +L+S Q + +K +++LRVLRPL+ +NR LK V CV +++ + NI+IV L +FA +GVQLF GKF CTD AK EEC+G + + P V ER W FN+DN +AM+ LF V T EGW +L ++ S E+ GP+ R E++IF+I+Y ++ FF +NIFV VIVTF + GE E + ++DKNQ VE
Sbjct: 557 ANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELE-LMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEETEDKDPYPPCDVPVGEEEEEEEEEPEVPAGPRPRRISELNMKEKIVPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISV-VKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENAGPVYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVE 1175 HSP 2 Score: 132.494 bits (332), Expect = 6.496e-31 Identity = 111/388 (28.61%), Postives = 178/388 (45.88%), Query Frame = 0
Query: 207 AAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDA---AARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ-------------GTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR--TAEECQGEYFSSGEWGQHIPEVAERE--WLIKA---FNYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFV 566
+ E ++ ++ V +AA+ A A KA ++ A + R+Q A ++ G + P +LF LS NP+R +V FD FI+ I + ALA P E+ N L K +Y F IF +E FLK++ GL+ HP ++ R+ WN++D +V L S+ + G G +K+++ RVLRPL++V+ VP+L+ V + ++ ++ + +I ++ + ++I+A++G++LF GK A AEE SG Q E W+ N+DN AM+T+F T EGW DVL ++M FE +PW Y V V+F FFV
Sbjct: 34 SGEGPTSQPNSSKQTVLSWQAAIDAARQA--KAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSR----------PARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAVNGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMG--FE----LPW-------VYFVSLVIFGSFFV 396 HSP 3 Score: 86.2705 bits (212), Expect = 3.046e-16 Identity = 146/655 (22.29%), Postives = 257/655 (39.24%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSG--------LRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN-------------------FDDG---TP--------TSNFN-----TAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYS------LYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF-QGTPT------------GQKLKS-------VKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDV----LQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVF----PFFFVNIFVALVIVTFNDL 580
L Y + F F E+L+++ G A+ + FN D V+ SL SFG+ LR LR+LR + L+++V + A+ +I +++ + L F+FA +G+QLF G F + DG +P S+FN +A+ AL TV T E W +++Y AI S G + G VY+ ++F++ + + ++N+F+ +T E+ EKE ++ LDK ++RQ + A L P PY F W V ++MFV+++ + A +++ N + + FT +F +E LKV+ F P +F D WN D +V ++I + + PT G +S +L RV+R +K+++R ++ + + S + + + ++ + I+A++G+Q+F GK A + + + F Q P+ A++ LF T E W ++ L + D P + T + F IVYF+ F F +N+FVA+++ F+ L
Sbjct: 917 LGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLV------------SFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNG--ENAGPVYNHRVEISIFFIIYIIIVAFFMMNIFVGFVI--------VTFQEQGEKE-----------YKNCELDK----------------NQRQCVEY------ALKARPLRRYIPKNPYQYKF-------------WYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVI----AFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESESLPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMF-GKV-----AMRDNNQINRNNNF------QTFPQ------------------AVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEY-TCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYL 1468 HSP 4 Score: 68.9366 bits (167), Expect = 8.358e-11 Identity = 134/653 (20.52%), Postives = 259/653 (39.66%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKP----------RAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF------------DDGTPT---------------------------SNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAE-LTANEEAEKERAVADAEAALQAEHAALDKAEG-------------GLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSS--TFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE 584
E FL F E +++ A G P AY + +N D ++ LF V+ L ++G F + LRA R+LR ++ L+ ++ +++ A+ ++ + L+ I I+A++G++LF G + +D P +NF+ A+LTV +T E W V+Y + G + +YFV L + G++ +LN+ L + + E A + +K R E L+ + +AE N + E + EG G+ G + L VT F ++ ++ L++ +++E N P ++ + A+F E +K+ LGL ++F ++N D G + L+ L +P G + + +R+LR K+ +L + ++ S+K++ ++L++ LF++IF+++G+QLF GKF + KR+ +DN A++T+F + T E W V+ + + + G I + I++I+ F+ + +N+F+A+ + ++L +AE
Sbjct: 164 EYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAVNGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFE-----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLRENEQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGSCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVT---FYWLVIVLVFLNTLTISSE---HYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQ----AYFVSLFNRFDCFVVCGGITETILVELELM-SPLG--VSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRST-------------------------------FDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMI------VCIYFIILFICGNYILLNVFLAIAV---DNLADAE 758
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|116241275|sp|Q01668.2|CAC1D_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 2; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 401.749 bits (1031), Expect = 2.849e-122 Identity = 268/622 (43.09%), Postives = 369/622 (59.32%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDD-GTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQA-EHAALDKAEGG--LNGDAAA-----RKEEEDRRQVADAE-------------------GGGGGAFGEDGL-EELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQVGPVE 598
A + L F EML+++Y+LG AYF S FNRFDC V+ G + E + V L+ + G+S R +RLLR+FKVT++W+ L NLV +L+N++ SI SLL LLFLFI IF+LLGMQLFGG FNFD+ T S F+ ALLTV LT EDWN+VMY I++ GG G++ +YF++L +CGNY LLNVFLAIA D+L A L ++ E E A ++ E+ +K E N D R+E+ED+ + G E + E++ PI S+ FILS TNP+RVG H L+ +F I+ I+LSSAALAAEDP++ ++ RN +L FDY FTAIF +E LK+ G H G+F R+ +N++D VV +L+S Q + +K +++LRVLRPL+ +NR LK V CV +++ + NI+IV L +FA +GVQLF GKF CTD AK EEC+G + + P V ER W FN+DN +AM+ LF V T EGW +L ++ S E+ GPI R E++IF+I+Y ++ FF +NIFV VIVTF + GE E + ++DKNQ VE
Sbjct: 558 ANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMS-PLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAIS-VVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVE 1177 HSP 2 Score: 130.954 bits (328), Expect = 2.351e-30 Identity = 111/370 (30.00%), Postives = 173/370 (46.76%), Query Frame = 0
Query: 223 ADAEAALQAEHA-ALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISL-------------YFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR--TAEECQGEYFSSGEWGQHIPEVAERE--WLIKA---FNYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFV 566
A +AA QA+ A + + G + RK R+Q A ++ G + P +LF LS NP+R +V FD FI+ I + ALA P E+ N L K +Y F IF +E FLK++ GL+ HP ++ R+ WN++D +V L S+ + G G +K+++ RVLRPL++V+ VP+L+ V + ++ ++ + +I ++ + ++I+A++G++LF GK A AEE SG Q E W+ N+DN AM+T+F T EGW DVL ++M FE +PW Y V V+F FFV
Sbjct: 55 AAIDAARQAKAAQTMSTSAPPPVGSLSQRK----RQQYAKSKKQGNSSNSR----------PARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMG--FE----LPW-------VYFVSLVIFGSFFV 397 HSP 3 Score: 85.1149 bits (209), Expect = 7.847e-16 Identity = 144/655 (21.98%), Postives = 256/655 (39.08%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSG--------LRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFN-------------------FDDG---TP--------TSNFN-----TAINALLTVLTEEDWNSVMYTAIISQGGRKGGGLVYS------LYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYF-QGTPTGQKLKSV-------------------KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDV----LQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVF----PFFFVNIFVALVIVTFNDL 580
L Y + F F E+L+++ G A+ + FN D V+ SL SFG+ LR LR+LR + L+++V + A+ +I +++ + L F+FA +G+QLF G F + DG +P S+FN +A+ AL TV T E W +++Y AI S G + G +Y+ ++F++ + + ++N+F+ +T E+ EKE ++ LDK ++RQ + A L P PY F W V ++MFV+++ + A +++ N + + FT +F +E LKV+ F P +F D WN D +V ++I + + PT + V +L RV+R +K+++R ++ + + S + + + ++ + I+A++G+Q+F GK A + + + F Q P+ A++ LF T E W ++ L + D P + T + F IVYF+ F F +N+FVA+++ F+ L
Sbjct: 919 LGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLV------------SFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNG--ENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVI--------VTFQEQGEKE-----------YKNCELDK----------------NQRQCVEY------ALKARPLRRYIPKNPYQYKF-------------WYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVI----AFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMF-GKV-----AMRDNNQINRNNNF------QTFPQ------------------AVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEY-TCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYL 1470 HSP 4 Score: 67.0106 bits (162), Expect = 3.201e-10 Identity = 134/653 (20.52%), Postives = 258/653 (39.51%), Query Frame = 0
Query: 11 ELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKP----------RAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF------------DDGTPT---------------------------SNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAE-LTANEEAEKERAVADAEAALQAEHAALDKAEG-------------GLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPR--NRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSS--TFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAE 584
E FL F E +++ A G P AY + +N D ++ LF V+ L ++G F + LRA R+LR ++ L+ ++ +++ A+ ++ + L+ I I+A++G++LF G + +D P +NF+ A+LTV +T E W V+Y + G + +YFV L + G++ +LN+ L + + E A + +K R E L+ + +AE N + E + EG G G + L VT F ++ ++ L++ +++E N P ++ + A+F E +K+ LGL ++F ++N D G + L+ L +P G + + +R+LR K+ +L + ++ S+K++ ++L++ LF++IF+++G+QLF GKF + KR+ +DN A++T+F + T E W V+ + + + G I + I++I+ F+ + +N+F+A+ + ++L +AE
Sbjct: 165 EYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFE-----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVT---FYWLVIVLVFLNTLTISSE---HYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQ----AYFVSLFNRFDCFVVCGGITETILVELEIM-SPLG--ISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRST-------------------------------FDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMI------VCIYFIILFICGNYILLNVFLAIAV---DNLADAE 759
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Match: gi|116407|sp|P22316.1|CAC1S_CYPCA (RecName: Full=Dihydropyridine-sensitive L-type skeletal muscle calcium channel subunit alpha-1) HSP 1 Score: 399.053 bits (1024), Expect = 1.055e-121 Identity = 250/622 (40.19%), Postives = 360/622 (57.88%), Query Frame = 0
Query: 10 AELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGT-PTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAV-----------ADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYS----------------------SLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
A ++ L F EM++++YA GP AYF S FNRFDC V+T + E++ V + G+S +R +RLLR+FK+T+YW+ L NLV +L+N++ SI SLL LLFLFI IFALLGMQ+FGG FNF D SNF+ AL++V LT E+W+S+MY I++ GG + G++ S+YF++L +CGN+ LLNVFLAIA D+L +A LTA ++ + E ++ E AL A+ +++ A K +E V + + A EE+ P +P S S FI + R H +V F I+ ILLSS +LAAEDP+ + RN++L+ D FT +F IE LK+ G H GSF R+ +NI+D VV +L+S+ + + T +K +++LRVLRPL+ +NR LK V C+ ++K + NI++V ML +FA +GVQLF GK YCTD ++TAEECQG + H EV +R W+ FN+DN M+ LF + T EGW ++L ++ S D GP+ R ++IF+I+Y ++ FF +NIFV VIVTF GE E D ++DKNQ
Sbjct: 482 ANVILLACFTIEMVMKMYAFGPRAYFMSIFNRFDCFVVTIGILEIILV-VSNIMTPLGISVMRCIRLLRLFKLTRYWTSLNNLVASLLNSVKSIASLLLLLFLFIVIFALLGMQVFGGKFNFPDRVIQRSNFDNFPQALISVFQVLTGEEWDSIMYNGIMAHGGPQSPGILVSIYFIILYVCGNFVLLNVFLAIAVDNLAEAESLTAAQKEKAEEKARRKLMRTLPEKSEEEKALMAKRLMESRSKAEGMPTTAKLKIDEFESNVNEVKDPFPPADFPGDHEEVEPEIPISPRPRPMADLQLKETVVPIAEASSFFIFGPQHKFRKLCHRIVNHTTFTNIILLFILLSSISLAAEDPIDPRSFRNKVLAYADIVFTTVFTIEIVLKMTVYGAFLHTGSFCRNSFNILDLIVVGVSLLSMGMESS-TISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAIKTIGNIVLVTMLLDFMFACIGVQLFKGKLYYCTDPLQKTAEECQGTFLKHVPNSLHDIEVHQRMWVNSDFNFDNVLNGMLALFTISTFEGWPEILYKAIDSNAVDTGPLYNNRVGISIFFIIYIIIIAFFMMNIFVGFVIVTFQKQGEQEYKDCELDKNQ 1101 HSP 2 Score: 123.25 bits (308), Expect = 7.171e-28 Identity = 100/352 (28.41%), Postives = 164/352 (46.59%), Query Frame = 0
Query: 243 LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLS--KFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ------GTPT----GQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGK------FLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAERE--WLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNI 568
+N + RK+ E +++ A GG P SLF + NP R +V F+ I+ I + ALA P+ E N L+ +Y F IF +ECFLK++ GL+FH G++ R+ WNI+D +VF L +L G PT G +K+++ RVLRPL++V+ VP+L+ V ++ S+ +F+I ++ + I+A++G++LF K + A R E +G + E W F ++DN AM+T+F T+E W DVL ++M + + PW I+++ +V FF +N+
Sbjct: 21 MNEEELKRKQREKLKKL-QATGGNPR--------------PPRSLFFFTLKNPFRKTCINIVEWKPFEIIILLTIFANCVALAVFLPMPEEDTNNTNLTLESLEYIFLVIFTLECFLKIVAYGLLFHEGAYLRNCWNILDFVIVFMGLFTLVVDTINTIAGVPTEKGGGFDMKALRAFRVLRPLRLVSGVPSLQVVMSSILKSMLPLFHIALLVFFMVHIYAIMGLELFKCKMHKTCYYQGTNIIAVREGNEKPSPCAQAGHGRRCTINGTECRAGWPGPNFGITHFDNSCFAMLTVFQCITTESWTDVLYWINDAMGNDW------PW------IYFLTLILVGSFFILNL 345 HSP 3 Score: 72.0182 bits (175), Expect = 8.997e-12 Identity = 63/237 (26.58%), Postives = 104/237 (43.88%), Query Frame = 0
Query: 12 LLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVN----LKPRAGSFGLSG----------------------LRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTP---TSNFNT---AINALLTVLTEEDWNSV----MYTAIISQGGRKGGGLVYS-------LYFVMLTLCGNYCLLNVFLAIACDSLD 205
L+F F EM+++L A YF +N FD ++ GS+ +V+ L+ R G + L G R R+LR+ K+ +RNL+ + + ++ + L+ + FI+A++GMQ+F G DGT +NF T A+ L V T E W V MY + G G Y+ YF+ + + ++N+F+AI D++D
Sbjct: 1171 LIFTVLFTGEMIVKLIAFKAKGYFGDPWNVFDFIIVVGSIVDVVLSEVDAALEARGGLWCLHGCAEVNPMQAIAEAENVRVSITFFRLFRVLRLIKLLNRSEGIRNLLWTFIKSFQALPHVGLLIVMLFFIYAVIGMQMF-GKVALVDGTEINRNNNFQTFPQAVLLLFRVATGEQWPKVILASMYGKLCDAKSDYGPGEEYTCGSSIAVFYFLSFYILCAFLIINLFVAIIMDNVD 1406
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1016161075|gb|KZC08237.1| (Voltage-dependent calcium channel type A subunit alpha-1 [Dufourea novaeangliae]) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 359/602 (59.63%), Postives = 439/602 (72.92%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARK--EEEDRRQVADAEGGGGGAFGEDGLEE----LRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L +AE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L+N++ SI+SLLFLLFLFI IFALLGMQLFGG FNFD GTP +NFNT ALLTV LT EDWN VMY I SQGG + G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A AE E+ V D E Q ++ + +G+A + + D +GG + + EE +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E++PRN +L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF YCTD +K T ++CQG+YF E G +PE +REW + F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 862 LYFAEFVFLALFMMEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFDYGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHRRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSA---AEDEQQVEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPNQNFKDGKGGKQSSEEKKQDEEDDTGPKPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFNMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDESKYTEQDCQGQYFVFEE-GAMLPEPKKREWHTQCFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1456 HSP 2 Score: 121.324 bits (303), Expect = 8.992e-25 Identity = 106/434 (24.42%), Postives = 192/434 (44.24%), Query Frame = 0
Query: 205 DQAAELTANEEAEKERAVADAE--AALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGG---------------------AFGED--GLEELRP--------------ILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMA-------IFYIVYFVVFPFFFVNIFVALV 573
D++ E NEE E +R V E + + E A +++ G + + +EE +R +A G +FG + GL ++ P +SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F IF +E LK+L LG + H GS+ R++WNIMD VV +++ F T L+ ++ RVLRPLK+V+++P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R C + +G H+ + W F ++DN G AM+T+F T EGW +L W + E+ I++I V+ FF +N+ + ++
Sbjct: 280 DESGESRGNEEDEAQRMVRRVEKGGSNEEEEDARIESQNGSERELSVDEEEPNRLAAHEAAQRDPGYASQYRRRPRLAGLSGLGDWTSFGGEVPGLVDMAPGRDQGGGAGGGGGGGKGTTSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGSMTV-FAETNVDVDLRMLRSFRVLRPLKLVSKIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDINFIMKDGEQPSPCNTDNKIEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILY--------------WVKQEICPSVTYNWIYFIPLIVLGSFFMLNLVLGVL 697 HSP 3 Score: 95.9005 bits (237), Expect = 1.089e-16 Identity = 133/624 (21.31%), Postives = 254/624 (40.71%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF-------------------NFDDG------------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFED----EGPIPWFRTEMAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EM++++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F++G T +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + N V+ I +V F+ FIM +I+L++ L + Q +A +N L + FT +F +EC LK+ G+ +FF+D WN D V +++ +L + + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L PE + + K N+ + ++ LF T E W +++ + + D + + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 1198 LNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFNMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDESKYTEQDCQGQYFVFEEGAMLPEPKKREWHTQCFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSCI----------------------------DFTIQARPLERY----MPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTLLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGVR----NFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL--------------------------DPESS----ITKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCIKGRPCDVKAGKQEPGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1735
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|987908412|ref|XP_015429783.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 [Dufourea novaeangliae]) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 359/602 (59.63%), Postives = 439/602 (72.92%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARK--EEEDRRQVADAEGGGGGAFGEDGLEE----LRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L +AE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L+N++ SI+SLLFLLFLFI IFALLGMQLFGG FNFD GTP +NFNT ALLTV LT EDWN VMY I SQGG + G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A AE E+ V D E Q ++ + +G+A + + D +GG + + EE +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E++PRN +L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF YCTD +K T ++CQG+YF E G +PE +REW + F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 483 LYFAEFVFLALFMMEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFDYGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHRRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSA---AEDEQQVEDKEKQAQELEKEIESLQKPKDGNAPKVEICPPSPNQNFKDGKGGKQSSEEKKQDEEDDTGPKPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVWEDSPRNEVLNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFNMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDESKYTEQDCQGQYFVFEE-GAMLPEPKKREWHTQCFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1077 HSP 2 Score: 119.398 bits (298), Expect = 3.541e-24 Identity = 85/311 (27.33%), Postives = 157/311 (50.48%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F IF +E LK+L LG + H GS+ R++WNIMD VV +++ F T L+ ++ RVLRPLK+V+++P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R C + +G H+ + W F ++DN G AM+T+F T EGW +L ++++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLGIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGSMTV-FAETNVDVDLRMLRSFRVLRPLKLVSKIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDINFIMKDGEQPSPCNTDNKIEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWVKDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 318 HSP 3 Score: 95.5153 bits (236), Expect = 1.533e-16 Identity = 133/624 (21.31%), Postives = 254/624 (40.71%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF-------------------NFDDG------------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFED----EGPIPWFRTEMAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EM++++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F++G T +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + N V+ I +V F+ FIM +I+L++ L + Q +A +N L + FT +F +EC LK+ G+ +FF+D WN D V +++ +L + + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L PE + + K N+ + ++ LF T E W +++ + + D + + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 819 LNYFDYAFTGVFTVEMILKIIDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFNMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDESKYTEQDCQGQYFVFEEGAMLPEPKKREWHTQCFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSCI----------------------------DFTIQARPLERY----MPKERNSVKYKIWRIVVSTPFEYFIMGLIVLNTLLLMMKFHRQSDAYKN-TLKYMNMCFTGMFTVECILKIAAFGVR----NFFKDAWNTFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL--------------------------DPESS----ITKHNNFQSFIQGLMLLFRCATGEAWPNIMLSCIKGRPCDVKAGKQEPGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1356
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101330|ref|XP_017877485.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X10 [Ceratina calcarata]) HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 339 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 934 HSP 2 Score: 100.138 bits (248), Expect = 5.000e-18 Identity = 143/643 (22.24%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 676 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1228 HSP 3 Score: 68.5514 bits (166), Expect = 2.937e-8 Identity = 49/192 (25.52%), Postives = 98/192 (51.04%), Query Frame = 0
Query: 402 LKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 3 LRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 181
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101320|ref|XP_017877479.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X5 [Ceratina calcarata]) HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.494 bits (332), Expect = 2.316e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 100.523 bits (249), Expect = 3.968e-18 Identity = 143/643 (22.24%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1366
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101318|ref|XP_017877478.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X4 [Ceratina calcarata]) HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.494 bits (332), Expect = 2.662e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 97.0561 bits (240), Expect = 4.333e-17 Identity = 137/627 (21.85%), Postives = 253/627 (40.35%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D V +++ +L + + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1350
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101328|ref|XP_017877484.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X9 [Ceratina calcarata]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.109 bits (331), Expect = 2.686e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 97.4413 bits (241), Expect = 3.685e-17 Identity = 137/627 (21.85%), Postives = 253/627 (40.35%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALI-SLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D V +++ +L + + ++L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1350
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101326|ref|XP_017877483.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X8 [Ceratina calcarata]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.494 bits (332), Expect = 2.612e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 100.138 bits (248), Expect = 4.958e-18 Identity = 143/643 (22.24%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1366
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101324|ref|XP_017877481.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X7 [Ceratina calcarata]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.494 bits (332), Expect = 2.617e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 100.138 bits (248), Expect = 4.965e-18 Identity = 143/643 (22.24%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1366
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101322|ref|XP_017877480.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X6 [Ceratina calcarata]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.494 bits (332), Expect = 2.466e-28 Identity = 89/311 (28.62%), Postives = 160/311 (51.45%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALV 573
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVL 319 HSP 3 Score: 100.138 bits (248), Expect = 4.602e-18 Identity = 142/643 (22.08%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F T+ +E N+ + A ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGN----ITNDPGTAIDEHN--------------------------NFQSFFAGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1366
BLAST of voltage-dependent p q type calcium channel vs. nr
Match: gi|1061101316|ref|XP_017877477.1| (PREDICTED: voltage-dependent calcium channel type A subunit alpha-1 isoform X3 [Ceratina calcarata]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 358/612 (58.50%), Postives = 437/612 (71.41%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALGPAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGSFGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFDDGTPTSNFNTAINALLTV---LTEEDWNSVMYTAIISQGGRKGGGLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGG----------------LNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQ--GTPTGQKL---KSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNDLGEAELTD-DIDKNQ 593
L YAE +FL F+ EM I++YALGP YF S+FNRFDC VI+GS+FEV+W LK +GSFGLS LRALRLLR+FKVTKYW LRNLVI+L++++ SI+SLLFLLFLFI IFALLGMQLFGG FNF+ GTP +NFNT ALLTV LT EDWN VMY I SQGG K G +VYSLYF++L L GNY LLNVFLAIA D+L A EL+A E E+ L+ E L K + G +G + EE+++Q D + G +P+LPYSS+FILS TNP+R HW+V FD FIM VI LSS ALAAEDPV E +PRN++L+ FDY FT +F +E LK++DLG++ HPGS+ R+ WNIMD VV CA +S F G+ GQ L KS+++LRVLRPLK + RVP LKAVFDCVV SLKNV NILIVY+LF IFA++ VQLFNGKF +CTD +K T EECQG+YF + G +PE +REW ++F+YDN AAM+TLFAVQT EGW +LQNSM++T+ED+GPI FR EM+IFYIVYF+VFPFFFVNIFVAL+I+TF + GEAEL D +IDKNQ
Sbjct: 477 LYYAEFVFLALFMLEMFIKVYALGPRTYFDSSFNRFDCVVISGSIFEVIWSALK--SGSFGLSVLRALRLLRIFKVTKYWKSLRNLVISLLSSMRSIISLLFLLFLFILIFALLGMQLFGGQFNFEAGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGTKRG-MVYSLYFIVLVLFGNYTLLNVFLAIAVDNLANAQELSAAENMEENEDKQKQAQELEKEIQTLQKPKDGNAPKVEICPPSPNQNFKDGKGEKQSSEEEKKQDEDDDTGP------------KPMLPYSSMFILSPTNPIRRAAHWVVNLRYFDVFIMVVITLSSIALAAEDPVWEESPRNKVLNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGM-LPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQ 1072 HSP 2 Score: 132.88 bits (333), Expect = 1.720e-28 Identity = 90/317 (28.39%), Postives = 163/317 (51.42%), Query Frame = 0
Query: 285 SSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAED--PVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMDGTVVFCALISLYFQGTPTGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKR----------TAEECQGEYFSSGEWGQHIPE----VAEREWLIKAF---NYDNCGAAMITLFAVQTSEGWVDVL---QNSMSSTFEDEGPIPWFRTEMAIFYIVYFVVFPFFFVNIFVALVIVTFND 579
+SLFILS N +R +++ P F+ ++ I+ + LA E+ P + + L + F AIF +E LK+L LG + H GS+ R++WNIMD VV I+ + QG L++++ +RVLRPLK+V+ +P+L+ V ++ ++ + I ++ + ++IFA++G++ ++G L+ T + R A C + S +G H+ + W F ++DN G AM+T+F T EGW +L +++ ST+ W I++I V+ FF +N+ + ++ F++
Sbjct: 22 TSLFILSEDNCIRKHTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPKHDKTILAQKLEATEIYFLAIFCVEASLKILALGFVLHRGSYLRNIWNIMDFFVVVTGFITAFSQGIELDMDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGT-LHKTCYSIRDISVIVKEGEQASPCNTDNKSEAPFGAHVCDANVSTCMDHWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTYN------W------IYFIPLIVLGSFFMLNLVLGVLSGEFSN 325 HSP 3 Score: 100.138 bits (248), Expect = 5.560e-18 Identity = 143/643 (22.24%), Postives = 256/643 (39.81%), Query Frame = 0
Query: 7 LVYAELLFLGAFVFEMLIRLYALG----PAAYFSSAFNRFDCAVITGSLFEVMWVNLKPRAGS--FGLSGLRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNF--------------------DDG-----------TPTSNFNTAINALLT---VLTEEDWNSVMYTAIISQGGRKGG----GLVYSLYFVMLTLCGNYCLLNVFLAIACDSLDQAAELTANEEAEKERAVADAEAALQAEHAALDKAEGGLNGDAAARKEEEDRRQVADAEGGGGGAFGEDGLEELRPILPYSSLFILSSTNPVRVGIHWLVTKPLFDGFIMFVILLSSAALAAEDPVQENAPRNRMLSKFDYGFTAIFFIECFLKVLDLGLMFHPGSFFRDVWNIMD-----GTVVFCALISL--YFQGTPTGQKLKSVK----------LLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFLYCTDAAKRTAEECQGEYFSSGEWGQHIPEVAEREWLIKAFNYDNCGAAMITLFAVQTSEGWVDVLQNSMSSTFEDEGPIPWFRTE-------MAIFYIVYFVVF-PFFFVNIFVALVIVTFNDL 580
L Y + F G F EML+++ LG P +Y +N D V+ + + AG + LR LR+LR K K L+ + ++N+L +++++L + LF FIFA++ +QLF G F F D+G T + +++ + A+LT V T E W ++ ++ + KG + S+++++ + + +N+F+A+ +T E+ E E + D E +DK + D + RP+ Y + V+ I +V F+ FIM +I+L++ L + Q + +N L + FT +F +EC LK+ G+ +FF+D WNI D G++V +I G P+G +L K L R R +K++ + ++ + V S K + + ++ + I+A++G+Q+F L DA + K N+ + ++ LF T E W ++ M S +D P + +A Y V F+ F F +N+FVA+++ F+ L
Sbjct: 814 LNYFDYAFTGVFTVEMLLKIVDLGIILHPGSYLREFWNIMDAVVVICAAVSFAFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFFCTDESKYTKEECQGQYFVFEDNGMLPEPRKREWSTQSFHYDNVMAAMLTLFAVQTGEGWPQILQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALII--------ITFQEQGEAE--LQDGE---------IDKNQKSC----------------------------IDFTIQARPLERY----MPRDRKSVKFRIWSIVVSTPFEYFIMCLIVLNTLLLMMKFHRQSDVYKN-TLKYMNMCFTGLFTVECILKIAAFGVR----NFFKDAWNIFDFITVIGSIVDALVIEFGERLSGMPSGGQLGEKKENFINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIAL---DAETS---------------------------ITKHNNFQSFLQGLMLLFRCATGEAWPNI----MLSCVKDRPCDPKASKQQGGCGSNIAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYL 1366 The following BLAST results are available for this feature:
BLAST of voltage-dependent p q type calcium channel vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 14
Pagesback to top
BLAST of voltage-dependent p q type calcium channel vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
Pagesback to top
BLAST of voltage-dependent p q type calcium channel vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold308_size214241:199420..212020+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold308_size214241-snap-gene-1.44 ID=maker-scaffold308_size214241-snap-gene-1.44|Name=voltage-dependent p q type calcium channel|organism=Tigriopus kingsejongensis|type=gene|length=12601bp|location=Sequence derived from alignment at scaffold308_size214241:199420..212020+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'voltage-dependent p q type calcium channel' has the following synonyms
Add to Basket
|