BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 206.453 bits (524), Expect = 1.922e-59
Identity = 159/566 (28.09%), Postives = 281/566 (49.65%), Query Frame = 0

Query:   45 SLQMSLVGSDNIVSAKTPVDHNHVLPTAPDRPMVEAYFL-GIKARIENEGNQRF-------YTDGVTG----KYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMF-HISGIGKLLMTAFNQQVIFF-----QDADLVQDISLILQACHKYRAGKVFLGSH--HCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNM-TLCNCYTMTELGFFVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVK-PPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHL-PGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            +LQ S +  + I++++   D + VL    +   + +YF+  +K  +    N+ F       Y  G T     + ++I D   K  + AK     G+ +GD+V I + N  +    V  T +C  V S++  ++S  T+ ++V++TQAK +ICT    R +++  D    S+ ++ L   +  L  I   D+   +Q+         P+ I +F L  +A+I W+SGTTG PKGIQ++   + + +  S    +  ++L TC F H+SG       + N  V  F     +D   +    +I           V   SH  + A+ +   ++    +   V+ + P GA  +  +  DL+  F N+  + N Y MTE G  +  S D    G V    +V I D  +   LGPN +GE++ + P  P  GY  +P ET +F   DG+C +GD  H+++ G L+Y+GR+KE++K   +H+YP+E+E +IQ +   + E G++ + +   Q +   +V +  G  +   EI++ V +++DD+K L   +   + PLPR+  GK++RN +
Sbjct:    3 NLQSSKINENKILTSQNDFDDSVVLEYNQN---ITSYFIDSMKKYMTKNSNKEFLVNCGKKYFPGETTSEGPESVSILDFIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTN---RTYYKVKD---LSSIIINL---DTQLHAILEIDMSTQTQI---------PEQI-EFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLLFLSPK--TDGNMLITCNFAHVSGFNYSCFWSINFDVCTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKI-QTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLE-IIILTNEPLPRNPNGKLLRNDI 542          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 155.606 bits (392), Expect = 1.823e-41
Identity = 146/566 (25.80%), Postives = 250/566 (44.17%), Query Frame = 0

Query:   45 SLQMSLVGSDNIVSAKTPVDHNHVLPTAPDRPMVEAYFLGIKARIE-NEGNQ-------RFYTDGVTGK---YLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISD-FSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPD-----LLTTCMFHISG-IGKLLMT-AFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG--FFVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFA-GYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHL-PGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            ++Q S +  D +++ K+  D N  L    ++ + E +   I   +E NE  +       RF+ D           + D   K   +AK     G+ + D+V + + N  +    V  T +C  V S++   +S  T+  ++ DTQA+++ CT    +K          +    +++ D +  + I N      S+++          P  D FSL D+ +I WSSGTTG PKG+Q++       M      F+ P      LL+    HISG I  L  T  F+    ++  +D V+   ++          K+F             EV                          LK   PN      Y  TE G  F VS +   N  G V   ++  I D  T   LGPN+ GE++ +     + GY  +  E  +  + DG+ HTGDF H+ EQG  ++  R+ E+IK + +H+YP+ELE L++ +   + EVGV    +   Q +  A++ +     +    I+K + ++VD++K++ G +     PLPR+A+GK++R+++
Sbjct:    3 NIQHSKISEDYVLTTKS--DDNRTLQAEYNQNLAECFLDKITKYLEVNEDKELLVNCGKRFFPDETVSDGPVAHTLKDFLHKVPALAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKV--------KNCGCTIINMDTDIEEMIANG---CTSKVIV---------PKQDVFSLKDVFVILWSSGTTGQPKGVQMTVVSFLDSM------FATPKVKGNMLLSANFGHISGFISSLFWTFHFSTSTFYYISSDDVKHFGVV----------KLF-------------EV--------------------------LKKCVPNF-----YGSTESGGGFLVSTNQHSN--GGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEK-KQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEI 483          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 123.25 bits (308), Expect = 5.477e-30
Identity = 118/470 (25.11%), Postives = 204/470 (43.40%), Query Frame = 0

Query:  144 FVPLVLGTWVCGAVPSLTDPDLSVATVRAQVE------DTQAKVIICTPGKVRKFHE-ALDQL--------HASTQLVLLHDDEENLQ-FIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMTAFNQQV--IFFQDADLVQDISL--ILQACHKYRAGKVFLGSHHCAQLVNMS-EVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTL-CNCYTMTELGFFVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLI-QHLPGVAEVGVYGQPDPASQELAVAVV---RLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            F+P+V GTW  G+  SL  P  S  T+  Q+       + +  V+I     + K     +D++        + S Q + ++DD E ++ F +     + S++  +     N        L+ I    +            V FQ+  H +  +    S   ++ T  FH  G  + L     Q+   I  +  +   D S   +  A    +   +        ++   S ++ S  DLS V ++YP+G  ++       +  FP++ + C  Y  TE    +S       LG +    Q++I++P     LGPN  G+IVVKP Y    Y     +       DGF + GD G++D  G +++  R K+++K  N+H+YP E+E+ I      V ++GV+GQ D    E    V+    +  G +L+    +   A +   +K L+GGVR V   +PR+A GK+ R +L
Sbjct:  108 FIPVV-GTWFLGSSVSLFQPSSSRETIERQLMILKEELNLEEIVVITETFAIPKISNIGVDRIISLTPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAEN-------ILNSIHYCSY------------VPFQI--HHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTET-LSISLGISDQHLGRLFPKYQLRILNP-NGEPLGPNSVGDIVVKPGYGMRRYIG---DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVR-VTSAIPRNANGKIFRGRL 549          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 111.309 bits (277), Expect = 6.649e-26
Identity = 113/537 (21.04%), Postives = 227/537 (42.27%), Query Frame = 0

Query:   90 ENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAI--ICWSSGTTG---TPKGIQVSF------------QLVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCN-CYTMTELGFFVSYSFDPNCLGSVAEGSQVKIVDP-VTKMAL-------------------GPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKLA 588
            E E ++ +  D   G+     ++     +VA   T  G  +GD++ +   N +E+  L LG W CG +    + +L    +  Q+ +++ K+++C         + L+  +   +L+++ +     +      + +++ LL    +    K I D++ D I +     SSG  G   T K +   F            QL+   +  +   F         +  I GI    ++ F++Q       + + D      +C+ ++            ++++ S + S  DLS ++++      + + +  ++    P++      Y + E G  V+ ++      S+A      + D  +  M L                   GP+Q GEI VK P    GY  +  E++ F D D + HTGD G++D QG++Y+  ++  +I++    + P  LE+ +     + +  V G P+  + ++  A V L PG    E  +   +  ++ D +++RGG+ FV   +PR    KV+R+ LA
Sbjct:   71 EKEPDRIWIKDLSIGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGN-----ECTAQDKVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSGAKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQNFL----EALVDXKTASISCYAWQI-----------RILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGL-VTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKE-ESSAF-DNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQ-NIPRDENWKVLRSVLA 583          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 107.071 bits (266), Expect = 1.317e-24
Identity = 66/178 (37.08%), Postives = 95/178 (53.37%), Query Frame = 0

Query:  410 PNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            PN +G        KI+D  T   LGP + GE+ V  P    GYYK    T +    +G+ HTGD  ++++ G  Y   R+KE+IK +   + P ELE+LI+  PGV +V   G PD  + EL  A V +    ++ E  I   V E+V   K+L  GV FVD  LP++  GK++R +L
Sbjct:  405 PNTIG--------KIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQ-NGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYV-VRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDT-LPKNQTGKILRREL 571          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 82.8037 bits (203), Expect = 1.107e-16
Identity = 67/233 (28.76%), Postives = 106/233 (45.49%), Query Frame = 0

Query:  359 LDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG--FFV-----SYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMR 584
            +D+S V L    G+     +  D +      +L N Y  TE G   FV     S+    N +G      + KIVD   K+ L  N  GE+ V+ P    GYYK    + +   PD + H+GD     E+G     GR+ +++     +IYP E+E+ +   P + EV + G PD    E   A + L  GA  +E ++K+H   ++  FK  +  +   D   P++  GKV +
Sbjct:  678 VDVSSVELGITGGSNCLVEVCEDFEKHLNMKSLMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGKI-LPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGA--KEFDLKEHCKGKIAHFKIPKYSMFVKD--FPKTLSGKVKK 905          

HSP 2 Score: 82.8037 bits (203), Expect = 1.272e-16
Identity = 65/239 (27.20%), Postives = 109/239 (45.61%), Query Frame = 0

Query:  359 LDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG--FFV-----SYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKLAYI 590
             ++  ++L    G+        D +      +L N Y  TE G   F+     S+    N +G      + KIVD   K+ L  N  GE+ V+ P    GYYK    + +   PD + H+GD G   E+G     GR+ +++     +IYP E+E+++   P + EV + G PD    E   A + L  GA  +E ++K+H   ++  FK  +  +   D   P++  GKV + ++  I
Sbjct:  307 FEMKAIKLGITGGSNCLVEACKDFEKHLNMKSLMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKI-LPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGA--KEFDLKEHCKGKISHFKIPKYPMFVKD--FPKTLSGKVKKFEMKAI 540          

HSP 3 Score: 79.337 bits (194), Expect = 1.237e-15
Identity = 62/207 (29.95%), Postives = 98/207 (47.34%), Query Frame = 0

Query:  391 LCNCYTMTELG--FFV-----SYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKLAYI 590
            L N Y  TE G   F+     S+    N +G      + KIVD   K+ L  N  GE+ V+ P    GYYK    + +   PD + H+GD G   E+G     GR+ +++     +IYP E+E+ +   P + EV + G PD    E   A + L  GA  +E ++K+H   ++  FK  +  +   D   P++  GKV + ++  I
Sbjct:  111 LRNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKI-LPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGA--KEFDLKEHCKGKIAHFKIPKYPMFVKD--FPKTLSGKVKKFEMKAI 312          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|75311763|sp|Q9M0X9.1|4CLL7_ARATH (RecName: Full=4-coumarate--CoA ligase-like 7; AltName: Full=4-coumarate--CoA ligase isoform 6; Short=At4CL6)

HSP 1 Score: 186.422 bits (472), Expect = 1.117e-50
Identity = 151/560 (26.96%), Postives = 260/560 (46.43%), Query Frame = 0

Query:   46 LQMSLVGSDNIVSAKTPVDHNHVLPTAPDRPMVEAYFLGIKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSL-----DDIAIICWSSGTTGTPKGIQ------VSFQLVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFFVSYSFDPN-------CLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            ++ S  G D I  +  P     VLP  P+  +V   F        +  ++    D  TG  L  S L+    ++A  F  +G+ + D+V I  PNS +F    L     G V +  +P  +V  V  Q++D+  K+II         ++  D++      V+L   ++ ++    ++    S++L+  + +   +P+S++        D A + +SSGTTGT KG++      ++  L+    +    ++    L    MFH+ G+  +  +   +       A    ++ L+L+   K+R   +++       L   S V    DLS ++ +    A +   ++ +     PN+ L   Y MTE    VS   DP          G +A G + +IV   T  +  PNQ GEI V+ P    GY   P  T    D   + HTGD G+F+E G LY   R+KE+IKY+   + P ELE L+   P + +  V   PD  + E+ +A V  +P +++ E +I+K +A+QV  +K+LR  V F+ + +P+SA GK++R +L
Sbjct:    1 MEKSGYGRDGIYRSLRP---TLVLPKDPNTSLVSFLF----RNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISV-------NQLFDKIKGFDLPVVLLGSKDTVEIPPGSN----SKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARF--ELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKK-FDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLR-RVSFISL-VPKSAAGKILRREL 536          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 182.185 bits (461), Expect = 4.545e-49
Identity = 146/513 (28.46%), Postives = 245/513 (47.76%), Query Frame = 0

Query:  100 DGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVII---CTPGKVRKFHEALDQLHASTQLVLLHDDEEN-LQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFN--QQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG-------FFVSYSFD--PNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            +G   +    +++E+  R+VA     +G+ + D + I +PNS EFV   +G    GA+ ++ +P  + A V  Q + + AK+II   C  GKV+ +    D      +++ +    E  L F +          L   D+   P+       DD+  + +SSGTTG PKG+ ++ +     + +     +AN +   + +  C   +FHI  +  +L+        ++  Q  D+   + LI     KY   KV +G      ++ +++ P     DLS VR V    A +   +   ++  FPN  L   Y MTE G        F    FD      G+V   +++KIVDP T  +L  NQ GEI ++      GY   P  TTR  D +G+ HTGD G  DE   L+   R+KE+IKY+   + P E+E L+ + P +++  V    D  + E+ VA V  + G+ + E E+K  +++QV  +K+++  V FV+  +P+S  GK++R  L
Sbjct:   51 NGANDQIYTYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKIIITQSCFVGKVKDYASEND-----VKVICIDSAPEGCLHFSE----------LTQSDEHEIPE--VKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELI----QKY---KVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVK-RVFFVET-VPKSPSGKILRKDL 537          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|57012620|sp|Q9LU36.1|4CL4_ARATH (RecName: Full=4-coumarate--CoA ligase 4; Short=4CL 4; AltName: Full=4-coumarate--CoA ligase isoform 5; Short=At4CL5; AltName: Full=4-coumaroyl-CoA synthase 4)

HSP 1 Score: 180.259 bits (456), Expect = 2.698e-48
Identity = 142/529 (26.84%), Postives = 250/529 (47.26%), Query Frame = 0

Query:   76 PMVEAYFLGIKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLS-QLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSAN------DFSAPDLLTTC--MFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFFV-SYSFDPN-------CLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            P+ +  F       + + +     DG TG+ L  +D++   R++A     +G+  GD+V + +PNS EF    L     GAV +  +P  +   +  Q + + AK+II     V K    L  L     L++  DD+ +   + ++D   +S   L   D+    KP    S +D   + +SSGTTG PKG+ ++ + +   +    +      +F+A D++     MFHI  +  L+++A               +++L+++   +Y+   V +        +   E     DLS VR++    A +   +   ++  FPN      Y MTE G    S +F  N         G+V   +++K+VD  T ++L  N++GEI V+      GY   P  T R  D DG+ HTGD G  D+   ++   R+KE+IK++   + P ELE L+   P + +  V    D  + E+ VA V  + G+ L E ++K +V +QV  +K+++  V F++V +P++  GK++R  L
Sbjct:   43 PLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASAAKMIITKKCLVDK----LTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETELLKP--KISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRF--ELNLVMELIQRYKVTVVPVAPPVVLAFIKSPETER-YDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIK-MVFFIEV-IPKAVSGKILRKDL 560          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 179.489 bits (454), Expect = 4.030e-48
Identity = 147/529 (27.79%), Postives = 247/529 (46.69%), Query Frame = 0

Query:   87 ARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVII---CTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTCM---FHISGIGKLLMTAFN--QQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVY----PVGARVHNAMLADLKAFFPNMTLCNCYTMTELG----FFVSYSFDP-----NCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            AR+   G +    DG TG+    +++E  +R+ A     MGVG+GD+V   + N  EF    LG    GA  +  +P  +   V  Q E   A+VI+   C   KVR+F        A   + ++  D      ++  ++    +L A  D  P          DD+  + +SSGTTG PKG+ ++ +     + +     + N + + D +  C+   FHI  +  +L+        ++  +  DL   + L+       R   + +       +V +++ P  +  DL+ +R+V     P+G  + +A +A +    PN  L   Y MTE G      ++++ +P        G+V   +++KIVDP T  +LG NQ+GEI ++      GY   P  T    D DG+ HTGD G  D+   ++   R+KEIIKY+   + P ELE L+   P + +  V    D  + E+ VA +    G+ + E EIKK VA++V  +K++   V F D  +P++  GK++R  L
Sbjct:   33 ARMAEVGARPCLIDGQTGESYTYAEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFA-------AERGVPVVTVDGAFDGCVEFREVLAAEELDADADVHP----------DDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLV-------RKHNITIAPFVPPIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDAFMAKI----PNAVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKRIN-KVFFTD-SIPKNPSGKILRKDL 531          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|112800|sp|P14912.1|4CL1_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 177.178 bits (448), Expect = 2.354e-47
Identity = 154/524 (29.39%), Postives = 250/524 (47.71%), Query Frame = 0

Query:   89 IENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPV----LSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFNQ--QVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFFVS---------YSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            I   G++    +G TG+    S +E+ +R+VA     +G+ +GD + + +PNS E+    LG    GA+ ++ +P  + A V  Q++ +QAK+II     V K  +              +  E+N+Q I   D P      S+L+   D+   P+ +   + DD+  + +SSGTTG PKG+ ++ +     + +     + N +   + +  C   +FHI  +  +L         ++  Q  D+V  + LI     KY   KV +G      ++ +++ P     DLS VR V    A +   +   ++A FPN  L   Y MTE G  ++         Y       G+V   +++KIVDP T  +L  NQ GEI ++      GY   P  T    D +G+ HTGD G  D+   L+   R+KEIIKY+   + P ELE L+   P +++  V    D  + E+ VA V    G T  E EIK+ V++QV  +K++   V FVD  +P+S  GK++R  L
Sbjct:   36 ISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLIITQACYVDKVKD--------------YAAEKNIQIICIDDAPQDCLHFSKLME-ADESEMPEVV--INSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELI----QKY---KVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIF-RVFFVDA-IPKSPSGKILRKDL 533          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 175.252 bits (443), Expect = 1.167e-46
Identity = 137/508 (26.97%), Postives = 239/508 (47.05%), Query Frame = 0

Query:   98 YTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISD-FSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPD-----LLTTCMFHISGIGKLL--MTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMS-EVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFF------VSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKLAYI 590
            + D  TG+ L  ++L      VA   + +G+ +G +V +  PNS+ F  + L     GA+ + T+P  +   +  Q++D+   +   T   + K   A  +L     +VL+  DEE +  + +     + +L+ +  + P+   + +    DD A + +SSGTTG  KG+  S + +   ++   N F + D     + T  MFHI G+      + A+   +I     ++ +    ++ A  KY+A  + L       +VN + ++ +  DLS +  V   GA +   +       +P + +   Y +TE          V  S      G ++   + +IVDPVT   LGP QTGE+ +K P    GY+     T+   D +G+  TGD  + DE G ++   R+KE+IKY+   + P ELE L+   P + +  V   PD    +  +A V    G++L E  I + VA+QV  +K++R  V FV   +P++  GK++R  L  I
Sbjct:   49 FIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKL----PIVLM--DEERVDSVGD-----VRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHE----MMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIR-KVAFVS-SIPKNPSGKILRKDLIKI 539          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|112801|sp|P14913.1|4CL2_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 175.252 bits (443), Expect = 1.236e-46
Identity = 154/524 (29.39%), Postives = 249/524 (47.52%), Query Frame = 0

Query:   89 IENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPV----LSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFNQ--QVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFFVS---------YSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            I   G++    +G TG+    S +E+ +R+VA     +G+ +GD + + +PNS E+    LG    GA+ ++ +P  + A V  Q++ + AK+II     V K  +              +  E+N+Q I   D P      S+L+   D+   P+ + D   DD+  + +SSGTTG PKG+ ++ +     + +     + N +   + +  C   +FHI  +  +L         ++  Q  D+V  + LI     KY   KV +G      ++ +++ P     DLS VR V    A +   +   ++A FPN  L   Y MTE G  ++         Y       G+V   +++KIVDP T  +L  NQ GEI ++      GY   P  T    D +G+ HTGD G  D+   L+   R+KEIIKY+   + P ELE L+   P +++  V    D  + E+ VA V    G T  E EIK+ V++QV  +K++   V FVD  +P+S  GK++R  L
Sbjct:   36 ISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLIITQACYVDKVKD--------------YAAEKNIQIICIDDAPQDCLHFSKLME-ADESEMPEVVIDS--DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELI----QKY---KVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIF-RVFFVDA-IPKSPSGKILRKDL 533          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 172.94 bits (437), Expect = 7.861e-46
Identity = 149/523 (28.49%), Postives = 247/523 (47.23%), Query Frame = 0

Query:   88 RIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDD--EENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFNQ--QVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG--FFVSYSFDPN-------CLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            R+    ++    DG T +    S++E+ +R+VA     +G+ +G +V + +PN +EF  + +G  V GA+ +  +P      +  Q +   A++I+     V K    L  L +   LV+  DD  +E  Q I      VL++  A   Q P  K       DD+  + +SSGTTG PKG+ ++ +     + +     + N +   D +  C   +FHI  +  +L+ A       +  Q  +L   + LI     KY   KV +       ++++++ P  S  D+S VR++    A +   +   L+  FP       Y MTE G    ++ +F  N         G+V   +Q+KI+D  T  +L  NQ GEI ++ P    GY   P  T    D +G+ HTGD  + D+   ++   R+KEIIKY+   + P ELE L+   P +A+  V  Q    + E+ VA V     + + E EIK+ VA+QV  +K++   V FVD  +P+S  GK++R  L
Sbjct:   34 RVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEK----LADLQSHDVLVITIDDAPKEGCQHIS-----VLTE--ADETQCPAVK----IHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELI----QKY---KVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFV--VKSSEISEQEIKEFVAKQVIFYKKIH-RVYFVDA-IPKSPSGKILRKDL 530          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|398963|sp|P31684.1|4CL1_SOLTU (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 170.629 bits (431), Expect = 4.991e-45
Identity = 150/527 (28.46%), Postives = 254/527 (48.20%), Query Frame = 0

Query:   91 NEGNQR-FYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVII---CTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPV----LSQLLAICD-QLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFN--QQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG-------FFVSYSFD--PNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            +E N R    DG   +    +++E+ +R+VA     +G+ + D + I +PN  EFV   +G    GA+ ++ +P  + A V  Q + + AK++I   C  GKV+ +                   E +L+ I    +P      S+L+   + ++P+ K       DD+  + +SSGTTG PKG+ ++ +     + +     +AN +   D +  C   +FHI  +  +L+ A      ++  Q  D+ Q + LI +        KV +G      ++ +++ P     DLS VR V    A +   +   ++A FPN  L   Y MTE G        F    FD      G+V   +++KIVDP T  +L  NQ GEI ++      GY   P  T R  + +G+ HTGD G  D+   L+   R+KE+IKY+   + P ELE L+ + P +++  V    D  + E+ VA V  + G+T+ E E+K  +++QV  +K+++  V FV+  +P+S  GK++R  L
Sbjct:   39 SEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVITQACFAGKVKDYA-----------------IENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVK----IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKH-------KVTIGPFVPPIVLAIAKSPLVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIK-RVFFVET-VPKSPSGKILRKDL 535          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|398965|sp|P31685.1|4CL2_SOLTU (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 170.244 bits (430), Expect = 7.423e-45
Identity = 147/526 (27.95%), Postives = 253/526 (48.10%), Query Frame = 0

Query:   91 NEGNQR-FYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVII---CTPGKVRKFHEALDQLHASTQLVLLHDDEENL----QFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTC---MFHISGIGKLLMTAFN--QQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVP--SGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELG-------FFVSYSFD--PNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            +E N R    DG   +    +++E+ +R+VA     +G+ + D + I +PN  EFV   +G    GA+ ++ +P  + A V  Q + + AK++I   C  GKV+ +      +    +++ +    E      + IQ+ +            ++P+ K       DD+  + +SSGTTG PKG+ ++ +     + +     +AN +   D +  C   +FHI  +  +L+ A      ++  Q  D+ Q + LI +        KV +G      ++ +++ P     DLS VR V    A +   +   ++A FPN  L   Y MTE G        F    FD      G+V   +++KIVDP T  +L  NQ GEI ++      GY   P  T R  + +G+ HTGD G  D+   L+   R+KE+IKY+   + P ELE L+ + P +++  V    D  + E+ VA V  + G+T+ E E+K  +++QV  +K+++  V FV+  +P+S  GK++R  L
Sbjct:   39 SEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVITQACFAGKVKDY-----AIENDLKVICVDSAPEGCVHFSELIQSDE-----------HEIPDVK----IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKH-------KVTIGPFVPPIVLAIAKSPLVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIK-RVFFVET-VPKSPSGKILRKDL 535          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|729357612|ref|XP_010545619.1| (PREDICTED: 4-coumarate--CoA ligase 4 [Tarenaya hassleriana])

HSP 1 Score: 214.157 bits (544), Expect = 2.942e-58
Identity = 149/514 (28.99%), Postives = 247/514 (48.05%), Query Frame = 0

Query:   93 GNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVII---CTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSAN------DFSAPDLLTTC--MFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFF---VSYSFDP-----NCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            G Q    DG TG+ L  SD+ V +R+VA    S+GVG+GD++ + +PNSLEF    L     GA+ +  +P  + A +  Q   + AK+++   C  GKV          +    +V+  +D  +  +++        + L   D+    +P  +   +D   + +SSGTTG PKG+ ++ + +   +    +      D+   D++     MFHI  +  LL++                ++SL+L+   +Y+     +       +V   E     DL  VR+V    A V   +   ++  FPN      Y MTE G     ++++ +P        G+V   +Q+KIVDP T+ +L  N  GEI ++      GY   P  T R  D DG+ HTGD G+ DE   L+   RMKE+IKY+   + P ELENL+  LP + +  V G  D  + E+ VA V  + G+ L E  +K  + +QV  +K+++  V F+D  +P++  GK++R +L
Sbjct:   49 GGQTCLIDGTTGRTLTYSDVHVASRRVAAGLQSLGVGKGDVIMLLLPNSLEFAVSFLAAAYLGAIATTANPLFTAAEIAKQSRASAAKMVVTQRCFAGKVAGLQ------NDGVLVVITDEDGPDEGYVR-------YKKLTESDETKLDRP--EIRPEDTVAMPYSSGTTGLPKGVMITHKGMVTSIAQKVDGENPNLDYKEGDVILCFLPMFHIFALDALLLSGIRAGAALLIVPRF--ELSLVLELIQRYKVTVAPVAPPVVLAVVKSPEAEK-YDLRTVRMVMCGAAAVKKELEEAVRLRFPNAVFGQGYGMTEAGTLAKSLAFAREPFEAKSGACGTVVRNAQMKIVDPTTRASLPRNHPGEICIRGDQLMKGYLNDPEATARTIDEDGWLHTGDVGYMDEDDELFIVDRMKELIKYKGYQVAPAELENLLISLPEIEDAAVIGMRDEVADEVPVAFVVRSQGSKLNEDGVKDFINKQVVHYKRIK-AVFFLDA-IPKAPSGKILRKEL 542          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|358031578|ref|NP_001239600.1| (luciferin 4-monooxygenase [Bombyx mori] >gi|355525889|gb|AET05796.1| luciferin 4-monooxygenase [Bombyx mori])

HSP 1 Score: 207.994 bits (528), Expect = 3.498e-56
Identity = 152/513 (29.63%), Postives = 247/513 (48.15%), Query Frame = 0

Query:   88 RIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVL--LHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ--LVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMT----AFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMT-LCNCYTMTEL-GFFVSYSFDPNCLGSVA---EGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            R+  E  +    +G +G+ L   ++  +   +A     +G+G+GDIV I   N  E++   +     GA  +  +   S        + ++ K I  +P   ++F+  L +L+   +  L  + DD++ + F           L        + +P      D  +II +SSGTTG PKG++++ +  LV    KP    F   DL+   +        +++T    + N+ V++       +D+   LQ   KY+AG + L       ++  S++    D+S V+++Y  GA + ++++  +K  F  +  +   Y MTE  G         N LGSV    EG+ VK+VD  T+  LGPNQ GEI VK P  F  Y  + +      D DGF  TGD  ++D +G  Y   R+KE+IKY+   + P ELE ++     V +VGV G PDP   EL  A V   P + + E E+   VA +V  +KQLRGGVRFV+  +P++  GK++R  L
Sbjct:   32 RLSKEKAKVALINGASGEKLTFGEMTQQIVNIASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINSAYSKNEFIHTTKISKPKYIFLSPSAYKEFYVTLKKLNIVQKFFLYGISDDKDVISF---------RDLATKYTHFNDFRPKDFNGTDSTSIILYSSGTTGLPKGVKLTHRNLLVSGLQKP----FCQKDLVILTLAPWCNTVGIILTLSCLSKNRTVLYLNK--FQEDV--YLQCIEKYKAG-ILLMVPPLLVILTKSKLIDKYDVSSVQIMYCGGAPLDSSVMTSIKKRFTGLKHVLQGYGMTETTGALTEEREHSNKLGSVGKVVEGNIVKVVDVETRKTLGPNQNGEICVKGPVLFEDYIGKDINED--LDEDGFYKTGDIAYYDNEGYFYIVDRIKELIKYKAGQVAPSELEAILLQHDAVQDVGVAGAPDPLVGELPTAFVVKKPNSKVTEKELIDFVAARVSSWKQLRGGVRFVN-EIPKTGSGKILRRIL 523          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1153828049|ref|XP_020290414.1| (4-coumarate--CoA ligase 1-like [Pseudomyrmex gracilis] >gi|1153828051|ref|XP_020290415.1| 4-coumarate--CoA ligase 1-like [Pseudomyrmex gracilis])

HSP 1 Score: 207.608 bits (527), Expect = 4.809e-56
Identity = 147/509 (28.88%), Postives = 249/509 (48.92%), Query Frame = 0

Query:   85 IKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLE-FVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICD-QLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVS-FQLVKHRMKPSANDFSAPDLLTTCMFHISGIG-KLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGF--FVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            I  ++ + GN+  + D  T + +   +L+VK  + A      G+GR D+V +   N     VP +  T+V  A+ +  + D+ ++T    ++  + K I CT   +    + L + + + ++V+  D+ +   F   +DI     L    D ++ N + +   SL   A I +SSGTTG PKG+++S + LV    + S  D +   L  + ++ ISG+   L       +V+ +     + D  +      K++   + L +   ++ +    V +   L  ++++Y  GA + ++M  +LK   P++ +   Y MTE+G              G+V    Q+KIVDP T   LGPNQ+GEI +K      GYY  P ET +  D DG+ H+GD G+ D+ G L+   R+KE++KYR   I P E+E ++   P V EV V G P     +  +A V   PGA + E E+  +VA  + D  +LRGGV F++     +  GK+ R  L
Sbjct:   31 IWDKLTSHGNKIAHLDARTEETVTYKELQVKTVKCALWLKQQGIGRDDVVSVCSNNHFNSIVPCLSATYVA-AIFNTMNEDMDLSTALHCIKLVKPKAIFCTAKPLDVIRKVLKETNYNIKVVVFDDNSDAFSF---SDI-----LNGYNDAEVANFRYVEHDSLKKTACILYSSGTTGMPKGVELSNYTLVNLAFEESV-DVNEVMLWFSSLYWISGVFLNLAAIVHGGKVLLYP----IFDEEMTCMLIEKFKVHTIVLSTSMVSRFLKAGYVKN-YSLPTLKILYVGGAILKSSMQENLKNLLPHVEILQAYGMTEVGIATLQKSKHKSGSSGTVVNNMQLKIVDPDTGKILGPNQSGEIWLKTTNMMNGYYNNPEETKKIIDKDGWLHSGDIGYVDDDGELFIWDRLKELMKYRGYQISPGEIEAVLSTHPAVYEVAVVGVPHELDNDHPLAFVTKKPGAKVTEQELIDYVANNMMDQYKLRGGVVFLE-SFSYTGSGKIARKHL 523          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|909562920|ref|XP_013136653.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Papilio polytes])

HSP 1 Score: 207.223 bits (526), Expect = 5.906e-56
Identity = 147/495 (29.70%), Postives = 243/495 (49.09%), Query Frame = 0

Query:  100 DGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDD--EENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMFHISGI-GKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPN-MTLCNCYTMTELGFFVSYSFDPN---CLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            +G T + L   ++  +A  +A     +GV RGD++ I   N  EF   V+G    G V +  +   +   ++  +  ++ K +IC+P   +   +    +     L++  ++  +  L +   A +   + +L    ++   + I     DD+A+I +SSGTTG PKG+ ++   V     P+        L  T  +H  G+   L+     +  I+    ++ Q     L+   KY+  ++   S     LV + + P+  D+S V +V    A +H      +   FPN   +   Y MTE    VS + +P+    +G V   S VKIV P T   LGPNQ GE+ VK      GY  +  +     D +GF  TGD G++DE   LY   R+KE+IKY+   + P E+E ++    GV +VGV G+P P++ E+ VA V   P + + EAE+++ VAE++ + K LRGGVRF+D  +P+SA GK++R  L
Sbjct:   41 NGATDEKLTYGEIAQEAMNLAVSLVKIGVKRGDVIAICSENRREFWSSVIGVICAGGVVTTINTSYTKDELKHVMGISKPKFVICSPFSYKAQEKNFKNIEGIKNLIIFGEEIPKNTLSYNALAIVGGSNSMLKENVKIDEFEAIEVVGKDDLAMILYSSGTTGLPKGVMITHLNVVTACSPTMEMPDIVALTITPWYHTMGLMSHLIGFVKGKMSIYLPKFEVEQ----YLRTIEKYKVSQL---SVVPPVLVALCKSPTKHDVSSVMIVVSGAAPLHKETADAVLKRFPNAQAVLQGYGMTEATLAVSINTNPDKKASVGQVTSNSVVKIVHPETGKVLGPNQEGEVRVKGVMLMKGYIGKKKQDD--FDEEGFFKTGDIGYYDEDKYLYIVDRLKELIKYKAYQVAPAEIEAVLLKHEGVRDVGVVGKPHPSAGEVPVAFVVTQPDSKVTEAELQQFVAERLSNTKHLRGGVRFIDA-IPKSATGKILRKDL 525          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1274123746|ref|XP_022826090.1| (4-coumarate--CoA ligase 1-like [Spodoptera litura])

HSP 1 Score: 206.068 bits (523), Expect = 1.287e-55
Identity = 146/498 (29.32%), Postives = 231/498 (46.39%), Query Frame = 0

Query:  101 GVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMF-----HISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNM-TLCNCYTMTELGFFVSYSF-----DPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            G T + L   +    A  ++   T +GVGRGDIV +      EFVP VL     GA  +  D     A ++ ++  T+ K  IC+      + + L         V L +  E++  I+         L+     +   +P+      DIA I +SSGTTG PKG+Q++   +   +    +DF+   L T  +F     +             +++++  +A+ +      +++ H       F+       L    E     +L  ++++Y   + +HN  +  +K+ FP +  +   Y MTE G   S S+         +G  + G  +K+VD  T+  +GPNQ GEI ++ P    GY   P   + + D DGF  TGD G+FDE    Y   R+KEII Y    + P+ELE ++Q  PGV E GV G+P     E   A V   PG  + E E+   VAE+V  F QLRGGVRF++  +PR+  GK++R +L
Sbjct:   42 GETREKLTYKEFTQYAVNMSTALTKLGVGRGDIVAVGSEKRNEFVPTVLAIVFTGATYTPYDLKSGRAVLKHKISLTKPKYFICSKLFWNTYSDILRSFDCIETFVTLDEGTESVISIRT--------LITNDADVSKFEPVQVQGQSDIAFILYSSGTTGMPKGVQLTH--LNCILNSLPDDFTDESLKTAFLFGEWYHNYDTFMTYKFLYLGRKIVYVNEAEPIS----FIKSVHDCDVNIAFIVPSFVCYLSKAKEAEK-YNLDSLKIIYSRSSPLHNKTIQKVKSRFPKVKNILQGYGMTEAGELTSESWGTKGPKSGSVGMASPGITLKVVDLETRKTVGPNQRGEICLRGPVLMKGYIGVP--PSNYLDEDGFFKTGDLGYFDEDRYFYIVERLKEIIVYDGYKVAPLELEAILQLHPGVREAGVIGKPVKVYGEEPTAFVVKQPGVNVTEKELLDFVAEEVPPFMQLRGGVRFIE-EMPRNPRGKIIRQRL 521          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1022777646|gb|KZS21172.1| (Luciferin 4-monooxygenase [Daphnia magna])

HSP 1 Score: 206.453 bits (524), Expect = 1.487e-55
Identity = 170/542 (31.37%), Postives = 264/542 (48.71%), Query Frame = 0

Query:   62 PVDHNHVLPTAPDRPMVEAYFLGIKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAK-LFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIP-VLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVS----FQLVKHRMKPSANDFSAPDLLTTCM---FHISGIGKLLMTAFNQQVIFFQDADLVQDISL--ILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGF-----FVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            P ++++V+P   D  + E Y L     IE  G+  + T+  T + L  S +  + + +A  L    GV   + + I +P+ LE+   VLG   CGA  +L +P  +++ ++  ++ T  KV I T     KFHE          L++L +          AD P   + LLA     P  +P  + S +D A+I +SSGTTG PKG+ ++        +  ++ + N    P+ L T M   +H  GI  +    F+  V   +   LV   +   +L+A  +YR   + +      QLV    V    DLS +RL++   A +   + A L   F    +   Y MTE        F+  S D   +G+V    +  +VDP T  ALGPN+ GEI V+ P    GY      T    D  G+ HTGD G++DE G  +   RMKE+IKY+   + P ELE ++ + P + E  V   PD A+ EL  A V   PG+TL E ++ K+VA++V   K+LRGGV F+   +P++A GK++R +L
Sbjct:   14 PGEYDNVIPE--DISLAE-YILN---EIEKFGDDIYSTNSETKQNLTFSQIVNQTKALAAGLQIKFGVQPKENIAIVLPSCLEYPVAVLGVNYCGAAATLINPSQTISELKHAIKLTNPKVWIGTEEFSTKFHELFLNAAQRPPLIVLTN---------KADHPSTWAGLLAFGQGKPVRRPTIN-SKEDTALILFSSGTTGVPKGVSLTHANYIAARRQNVELTKNIPKNPEDLNTVMLPLYHTFGISSI----FDNMVRGLRFI-LVPHFTFKNMLEAIQEYRISIMSVVPAIATQLVKQP-VEQHYDLSSLRLLFSGAAALSKEIQAGLVEKF-GCFVFQGYGMTESTLRTHSNFIGSSRD-GSIGTVMPFCESIVVDPDTNRALGPNEEGEICVRGPLIMKGYIGDEEATRHTIDSQGWLHTGDIGYYDEDGFFFITDRMKELIKYKGLQVSPTELEQILLNHPEIMEAAVAPVPDEAAGELPRAYVVKLPGSTLTEDDVAKYVADKVSPHKRLRGGVVFIK-SIPKTATGKILRREL 530          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325261085|ref|XP_023330441.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 206.453 bits (524), Expect = 1.516e-55
Identity = 148/529 (27.98%), Postives = 253/529 (47.83%), Query Frame = 0

Query:   70 PTAPDRPMVEAYFLGIKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQ-----LVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNM-TLCNCYTMTELGFFVSYS---FDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDP--ASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            P  P++P +E     I   +++ G++ +  D  TG    IS +E  +R+VA    S+G G GD++Q    + L+F   V G W+CG   SL DP+LS   ++ Q+E+T+AKV++ +   + K+++ + +L    + V     +   Q      +     L+ + +    P  + DF+ D  +II WSSG++GTPKGI  S +        H +KP     SA       MFHIS     L     +  I +   +       +L+   K +   VF G     Q+ N     +G +L+ V+ + P+G  V       + +   +  TL   Y  TE+ F   +     +   LGS+A G ++ I D  T   L P + G+I+VK       Y  +  ET  F D DGF  TGD G++D  G +++  RM+E++K  N    P E+ENL++  P + E  V+G+ DP   + ++ + +V+        +A+I   V   +   +++ G + FV+  +P +  GK +R ++
Sbjct:   17 PEQPNQPYIERLLSEINQELDDTGDRDWLVDTETGSSRTISQIEPISRRVASALLSLGFGPGDVLQTGYSSCLDFYWPVFGAWLCGGTVSLGDPNLSETNIKKQLEETKAKVLVSSIDYLDKYYKVIQELKLQGKNVKYFILDAGEQDTLPLGVESFQTLMRVQETQKMP-LLLDFTPDRESIIMWSSGSSGTPKGILHSQKNLLNLYFGHDLKPKTIISSA------LMFHISSFTFNLTLGILKGTICYIMKEKCFSGENLLKVAEKIQPEHVFCGVSQYIQVSNTK--TNGYNLNGVKNITPLGGAVSPGCSEKVLSLLGSQATLVEMYGSTEVMFVSKHEVKDIEFGLLGSLAAGVEICIQDINTGERLDPGKEGKIMVKTNTMMMRYLNREKETNEFFDSDGFGFTGDIGYYDNFGKIFFSYRMREVLKVDNYWFGPAEIENLLEKEPTIDEACVWGEYDPNTGNDQVNLGIVQKQNQKEWTKAKICSLVDANLPCTRKITGKIYFVE-KIPHNPQGKKLRGEM 535          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1330905577|gb|PNF29545.1| (Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905578|gb|PNF29546.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905579|gb|PNF29547.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905580|gb|PNF29548.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905581|gb|PNF29549.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905582|gb|PNF29550.1| Luciferin 4-monooxygenase [Cryptotermes secundus])

HSP 1 Score: 206.068 bits (523), Expect = 1.807e-55
Identity = 152/512 (29.69%), Postives = 244/512 (47.66%), Query Frame = 0

Query:   91 NEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLV-----LLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVS----FQLVKHRMKPSAN--DFSAPDLLTTCMFHISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLCNCYTMTELGFFV----SYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
              GN     D VTG+    SD+  ++  VA+     GV  GD+V I   NSL+F+  VL T+  GA+ +  +P+ +   +   +  ++ ++I C+   +    E    +    ++V     + H     + F++              D+  + +P+ +  ++ +A I  SSGTTG PKG+ +S      L+++  +PS N  D     L  T MFH  G   +L+ A     +   D     +  + L++   ++   +FL       L   +E+    DLS V+ +Y   A +   +   +K       +   Y +TE    V             G++  G   K+VD  T   LGP++TGE+  K P+   GYY  P  T    D DGF  TGD G++DE G  Y   R KE+IKY+   + P ELE ++   P V + GV G PD A+ EL +A +   PGATL + EI K+VA+ V   K+LRGGV FV+  +P+++ GK++R +L
Sbjct:   31 RHGNAVAMVDAVTGETRTFSDILTRSLSVAECLRVRGVAIGDVVAICSENSLDFIVPVLATYYIGAICAPMNPNYTAHEMLHVINISKPRIIFCSGKALACVDEVSRHVGFLKEIVTFGLPMSHKHTPFVTFLR--------------DKSCSFQPLDEHDMNLVAAILCSSGTTGLPKGVMLSQKNIISLLEYVSEPSINFIDNREVTLGLTPMFHAYGFMFMLLLATAGSKVVVMDR---FEEHIFLRSIQNHKIKHLFLVPPLVVFLAK-AEIVDKYDLSSVQRIYSGAAPLSEEIETLVKKRLGVDLVIQAYGLTETTLAVIGPTENENKKGTAGTLRPGVTCKVVDLETGRTLGPHKTGELRFKGPFIMKGYYGDPQATAASFDSDGFLLTGDVGYYDEDGFFYIVDRAKELIKYKGYQVPPAELEAVLLTHPAVKDAGVVGIPDEAAGELPLAFIVKQPGATLTKEEIIKYVADHVSPQKRLRGGVLFVE-SIPKTSSGKILRREL 523          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 205.297 bits (521), Expect = 4.018e-55
Identity = 155/541 (28.65%), Postives = 255/541 (47.13%), Query Frame = 0

Query:   54 DNIVSAKTPVDHNHVLPTAPDRPMVEAYFLGIKARIENEGNQRFYTDGVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQL----HASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMFHISGIGKLLMT----AFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNMTLC-NCYTMTELGFFVSYSFDPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAV-AVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMR 584
            D ++ +K P    + L    +R + +   L I+A +   G++ +  D  T   + IS +  ++ +VA   T +G   GD +  +    L+F   + G W+CG V S+ DP LS + +++Q++DT AK+I+C      KF EA   L     AS  LVL   D E  Q I        S      DQ P+   +  +   +  +I W+SGTTG+PKG+ +S + +  +M+ S        L +   FH    G  L++      N+  I F        +  I +   KY+      G++   QL   S   +  DLS V ++ P G ++  +    +K  FPN+      Y  TE+G  VS SF    +G++ EG Q  I D  T   LG  + GEI+ K P     Y  +  E+    D +GF H GD G++DE G+L+Y  R+KE+IK  N  I P E E+ ++++PGV E  V+   +  +    +   V L   + L   +I +   + V+  +++ G +  ++  +P +  GK +R
Sbjct:    6 DKVLYSKEP----YTLDPGQERIIFKLLDL-IQAELRETGDRDWILDCTTENSVRISHILGRSIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPIFGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVL---DLEPWQPIPEGTSSFRSLYEDQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQSKLPEKTIGLASNTFFHA---GSFLLSLDGGIRNKFSIHFIQPKHTT-VESIFRFIEKYKPMFFMCGANDFVQLA--SSDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGG-VSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVWQSMNKETDNYYIHGAVTLQASSGLNPEKILELANKDVEVKRRIDGTIHIIN-NMPHNPQGKKLR 530          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1247028125|gb|PCG66062.1| (hypothetical protein B5V51_8269 [Heliothis virescens])

HSP 1 Score: 202.601 bits (514), Expect = 3.189e-54
Identity = 145/498 (29.12%), Postives = 231/498 (46.39%), Query Frame = 0

Query:  101 GVTGKYLNISDLEVKARQVAKLFTSMGVGRGDIVQISVPNSLEFVPLVLGTWVCGAVPSLTDPDLSVATVRAQVEDTQAKVIICTPGKVRKFHEALDQLHASTQLVLLHDDEENLQFIQNADIPVLSQLLAICDQLPNPKPISDFSLDDIAIICWSSGTTGTPKGIQVSFQLVKHRMKPSANDFSAPDLLTTCMF-----HISGIGKLLMTAFNQQVIFFQDADLVQDISLILQACHKYRAGKVFLGSHHCAQLVNMSEVPSGLDLSCVRLVYPVGARVHNAMLADLKAFFPNM-TLCNCYTMTELGFFVSYSF-----DPNCLGSVAEGSQVKIVDPVTKMALGPNQTGEIVVKPPYPFAGYYKQPLETTRFCDPDGFCHTGDFGHFDEQGLLYYDGRMKEIIKYRNNHIYPMELENLIQHLPGVAEVGVYGQPDPASQELAVAVVRLAPGATLREAEIKKHVAEQVDDFKQLRGGVRFVDVPLPRSAVGKVMRNKL 587
            G T + L   +    A  ++   T +GVGRGDIV +      EFVP VL     GA  +  D     A ++ ++  T+ K  +C+      + + L       + V L D  E++  I+         L+     +   +P+      DIA I +SSGTTG PKG+Q++   +   +    +DFS   L T  +F     +             +++++  +A+ V      L+          F+       L    E     +L  ++++Y   + +H+  + ++K+ FP +  +   Y MTE G   S S+         +G  + G  +K+VDP T+  LGP Q GEI ++ P    GY   P   + + D DGF  TGD G++DE    Y   R+KEII Y    I P+ELE ++Q  PGV E GV G+P P   E   A +    G  + E E+  +V+ +V  F QLRGGVRF++  +PR+  GK++R +L
Sbjct:   42 GDTREKLTYKEFTQHAVNMSVALTKLGVGRGDIVAVGSEKRNEFVPTVLAIVFTGATYTPYDLKSGRAALKHKISLTRPKYFVCSNHFWNTYSDILKSFDCIEKFVTLDDGTESVISIRT--------LVTDDADVNKFEPVQVQGQTDIAFILYSSGTTGMPKGVQLTH--LNCILNSLPDDFSDESLKTAFLFGEWYHNYDTFMTYKFLLLGRKIVYINEAEPVT----FLKTARDCDVNIAFIVPSFVCYLSKAKEAEK-CNLDSLKIIYSRSSPLHDKTIQEVKSRFPKLKNILQGYGMTEAGELTSESWGTKGPKSGSVGMASPGITLKVVDPETRKTLGPKQRGEICLRGPVFMKGYIGVP--PSHYIDEDGFFKTGDLGYYDEDKYFYIVERIKEIIVYDGYKIAPLELETILQLHPGVKEAGVVGKPVPVYGEEPSAFIVKQHGVDVTEQELIDYVSTEVPPFMQLRGGVRFIE-EMPRNPRGKIIRQRL 521