BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 210.69 bits (535), Expect = 2.401e-61
Identity = 153/555 (27.57%), Postives = 270/555 (48.65%), Query Frame = 0

Query:    5 KDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSR--KTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQI--NEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG-TRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKL-PGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            ++K+L S+   D+S++ +   +++       +++ +  N + +FL+NC  G +    +         S ++ + +  K AK L   G    DV+H+ + N  + + PV  T LCQGVVS+   ++  E +  ++ E++AKFV+C      +V+    DL   I+       + D  +++  +               D+     I+W+SGTTG PKGIQ +   + +++     P     +  +  F H  GF +     S     C F   + E+     +G  K+I   +       ++  SH      +I  ++++   + D V+ ICP G +      + L  +F  L+NV NLY++TE G LI  S   R  G V     V I D  + E LG N  GE++ +   +  +GYLNRP ET+     DG+  +GD+ HY++ G +F++GR+KE++K    H+YP+EIE II+ +   + E  +F K + I Q ++  +VV  +G  ++ + I   V   +DDYK +   ++    LPRN  GK++R  +  +
Sbjct:   11 ENKILTSQNDFDDSVVLE-YNQNITSYFIDSMKKYMTKNSNKEFLVNC--GKKYFPGETTSEGPESVSILDFIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKVK----DLSSIII-------NLDTQLHAILEIDMSTQTQIPEQIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLL--FLSPKTDGNMLITCNFAHVSGFNYS-CFWSINFDVCTFHFISPEDVTS--LGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKI-QTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRNDINKL 545          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 167.933 bits (424), Expect = 1.539e-45
Identity = 143/500 (28.60%), Postives = 237/500 (47.40%), Query Frame = 0

Query:   77 MSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATR--DLMVKILVV--RDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQR---MAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALE-NTEQGLVGNSKLI----------LDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR------HLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            +S+K    L  +G +  DV+ L +PN  E+    +G       ++  +P  +PE +  Q+  S AK+V+     L  ++++      + KI+V+   + P D  + +     ++A  DD  F      ID     +A+ +SSGTTG PKG+  +   + + I +   P        S   F G      IS+        FF  +A+  +   GL    KLI          +++  +Y+P  M++       ++    + P L L S++ +    +       E+      +       Y +TE   +      P+        G++ P T  KI+D DT + LG  + GE+   G +IM GY    K T+  +  +G+ HTGD+ +Y++DG  +   RLKELIK +   + P E+E++IRK PGV +VA  G PD  + EL  A VV+   S V E+ I+D VT+ V  +K++  GV+FV +LP+N  GKI+RR +K 
Sbjct:   96 LSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFM-----EMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHP--------SIHNFIGDEVEQHISI----AVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSL-LSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVT--HVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQ-NGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRS-VMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 573          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 136.732 bits (343), Expect = 1.152e-34
Identity = 130/491 (26.48%), Postives = 219/491 (44.60%), Query Frame = 0

Query:   98 LALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMV---------KILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINR----GYGPLNAQTIFQS-----------TCFFHGGGFGFPI-SVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLS-QINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEII-RKLPGVSEVAVFGKPDPISQELVTAVVVK---SEG---SNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMYS 555
            L L NT  +F PV+GT+     VSL  P    E +  Q++  + +       NL ++   T    +         +I+ +   P       Y   Q++   DD + V           I    GTT +   I        +I+N      Y P     + ++           T FFH GGF   + ++L   +T        L +  + +  ++  + +A    +P  ++     A++++   N++  Q DL SV  I P+G +I  +  ++    FP+LK +   Y  TE  + I+   + +HLG + P   ++I++ +  E LG N VG+I+ K    M  Y+    + E  +  DGF + GD+G+YD  G +FF  R K+L+K  N H+YP EIE  I  K   V ++ VFG+ D    E ++ V+V     +G    NV E+T           YK ++GGV    ++PRN+ GKI R  +  +++
Sbjct:   99 LLLNNTRFFFIPVVGTWFLGSSVSLFQPSSSRETIERQLMILKEEL------NLEEIVVITETFAIPKISNIGVDRIISLTPVPG------YMSLQEIFMNDDNEVVE----------IFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGII-----LRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLS-ISLGISDQHLGRLFPKYQLRILNPNG-EPLGPNSVGDIVVKPGYGMRRYIG---DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERT---FEAYKFPRYKWLQGGVRVTSAIPRNANGKIFRGRLPLLFN 554          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 134.42 bits (337), Expect = 1.041e-33
Identity = 132/515 (25.63%), Postives = 221/515 (42.91%), Query Frame = 0

Query:   69 RMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWS--SGTTGMPKGIQHSFRMIAD-------IINRGYGPLNAQTIFQSTCFFHGGGF-GFPISVLSGGQTACFFPTYAL-ENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICP----MGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVD--------------------SDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMR 547
            R G+ +  +  K A  L   GF   DVL L   N  EY+   LG + C G+V   + +L P+ +  Q+ ES+ K +VC   N         +  M++ L++        + V      L   DD + V   +  D    +I+   + T+   KGI H+ + +           N  +  L   +I  +  FFH  GF  F +  + G         Y L E +EQ         L+A    +   +   +     LSQ + +    DL S++ +      + + I  +  +R+    P++K V   Y L E G L+T +       S+A      + D                     D     G +Q GEI  K  + M+GYLN  KE  +  ++D + HTGDLG+YD  GI++F  ++  LI+F    + P  +E  +     + + AV G P+  + ++  A VV   G    E+ + +L+   + D++RIRGG+ FV ++PR+   K++R
Sbjct:   89 RFGN-VHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLLN--DDGKKVPQKVIFDWAKDVIYMPFTSTSSGAKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYG------ISIYTLSEFSEQNF-------LEALVDXKTASISCYAWQIRILSQ-SPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRI----PSIKYVREAYGLNECG-LVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLN--KEESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLR 579          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 127.487 bits (319), Expect = 8.724e-32
Identity = 75/211 (35.55%), Postives = 114/211 (54.03%), Query Frame = 0

Query:  348 VFPALKN-VFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIM-AKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKL-PGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPS-LPRNSIGKIMRRHVKTMY 554
            +F  LK  V N Y  TE G     ST     G V   T   I D +T + LG N++GE+M  K      GYLNR +E E     DG+ HTGD  HY E G  FF  R+ E+IK +  H+YP+E+E +++ +   + EV V    +   Q ++ A++V      +N +TI   + + VD+YKRI G ++     LPRN++GK++R  ++ ++
Sbjct:  278 LFEVLKKCVPNFYGSTESGGGFLVSTNQHSNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEK-KQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQKLF 487          

HSP 2 Score: 81.6481 bits (200), Expect = 1.131e-16
Identity = 56/220 (25.45%), Postives = 99/220 (45.00%), Query Frame = 0

Query:    5 KDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSM---GFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQ 221
            +D VL +K  +DN  +     ++L E    K+ + +E N D + L+NC    +    DE   D  +   ++    K   L +S+   G    DV+HL + N  + + PV  T LCQGV+S+    +  + +  ++ +++A+ V C      +V+     ++       +   D +  + + C     V       D   +     I+WSSGTTG PKG+Q
Sbjct:   11 EDYVLTTK-SDDNRTLQAEYNQNLAECFLDKITKYLEVNEDKELLVNCG---KRFFPDETVSDGPVAHTLKDFLHKVPALAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKVKNCGCTII-------NMDTDIEEMIANGCTSKVIVPK----QDVFSLKDVFVILWSSGTTGQPKGVQ 215          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 80.1073 bits (196), Expect = 6.985e-16
Identity = 58/210 (27.62%), Postives = 101/210 (48.10%), Query Frame = 0

Query:  352 LKNVFNLYSLTEFGALITFSTTPR-----HLGSVAP---GTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            +K++ N+Y  TE G  ++F   P+      + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDLG   E G     GRL +++    +++YP EIE+++ K P + EV + G PD    E + A +   EG+   E  + +     +  +K I    +FV   P+   GK+ +  +K +
Sbjct:  336 MKSLMNIYGATETGP-VSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGA--KEFDLKEHCKGKISHFK-IPKYPMFVKDFPKTLSGKVKKFEMKAI 540          

HSP 2 Score: 78.5666 bits (192), Expect = 2.120e-15
Identity = 60/215 (27.91%), Postives = 100/215 (46.51%), Query Frame = 0

Query:  347 GVFPALKNVFNLYSLTEFGALITFSTTPR-----HLGSVAP---GTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
             V  A K + N+Y  TE G  ++F   P+      + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDLG   E G     GRL +++    +++YP EIE+ + K P + EV + G PD    E + A +   EG+   E  + +     +  +K I    +FV   P+   GK+ +  +K +
Sbjct:  103 AVLEAQKKLRNIYGATETGP-VSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGA--KEFDLKEHCKGKIAHFK-IPKYPMFVKDFPKTLSGKVKKFEMKAI 312          

HSP 3 Score: 76.6406 bits (187), Expect = 7.578e-15
Identity = 65/243 (26.75%), Postives = 112/243 (46.09%), Query Frame = 0

Query:  321 QLDLDSVRYICPMGTN----IPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR-----HLGSVAP---GTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVK 551
            ++D+ SV      G+N    + ED  + L+     +K++ N+Y  TE G   TF  +P       + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDL    E G     GRL +++    +++YP EIE+ + K P + EV + G PD    E + A +   EG+   E  + +     +  +K I    +FV   P+   GK+ +  ++
Sbjct:  677 KVDVSSVELGITGGSNCLVEVCEDFEKHLN-----MKSLMNIYGATETGP-ATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGA--KEFDLKEHCKGKIAHFK-IPKYSMFVKDFPKTLSGKVKKLEMR 909          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 63.929 bits (154), Expect = 8.188e-11
Identity = 84/370 (22.70%), Postives = 152/370 (41.08%), Query Frame = 0

Query:  207 IIWSSGTTGMPKGIQHSFRMIADIIN---RGYGPLNAQTIFQSTCFFHGGGFGFPISV-LSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFG-----ALITFSTTPRHLGSVAPGTTVKIVD---------SDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACL--NSDGFLHTGDLGHYDEDGIMFFDGRLK-ELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMYS 555
            I+++SGT+G PKG+  + R I   ++   + +   +   I      +H  G    + V LS G      P +  +     L  NS + +           +L   + +      E+   L   + R +     ++PE    +   +   +  +   Y +TE G     +L      P  +G+  P    +I+          +D + +  +++ GE+  +G  +   Y    K T+     N D FL TGD    +  G+    GR   ++IK     L  +EIE      P +SE+AV G  D I  E+V A++V ++G  +N   +     + +  Y   R   + V S+ +N +GKI ++ +K M S
Sbjct:  152 ILYTSGTSGSPKGVWITHRNIEAQVSGMIKFWAWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLT-NSSMNISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLR-STYRLMVCGSASLPEPLLRKWQTISGHI--LLERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFL-TGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYSVPR-QFMLVNSIDKNHMGKINKKELKKMDS 515          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 60.077 bits (144), Expect = 1.155e-9
Identity = 110/484 (22.73%), Postives = 182/484 (37.60%), Query Frame = 0

Query:   81 TAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL-----MVKILVVRDCPEDPDNHV--YSFCQQLAKVDDAQFVSD---HLDIDQRMAIIWSSGTTGMPKGIQHSFRMI---------------ADIINRGYGPLN---AQTIFQSTCFFHGGGFGFP---------ISVLSGGQTACFFPT-YALENTEQGLVGNSK-----------LILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFE-NKHLYPMEIEEIIRK-LPGVSEVAVFGKPDP-ISQELVTAVVVKSEGSNVNEKT 512
            TAK    +G +    + +   N  E+F   LG     G+ +   P        Y +  SR + +V    NL  +R  +  L     + KI+V+ +    P  ++  +    +L + +    ++D   H+ ++Q  ++I++SGTTG PKG+  S  +I               A  I   Y PL+   A  +      +  G   F          +  L   +   FF      E  ++G+   SK           +  DA+  Y   G     H         +V   L LD        G  + E T +    +   ++  + L     F ++          G++ P T  K+V S + + +G    GEI  KG  +MMGYL   + T    + +G+ HT               GRLK+L+     +++ P  IE  I+  LP VS   V G  +  IS  L   V    +G   +E T
Sbjct:   87 TAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVE--NLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKS-SDDDIG----GEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELT 548          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 56.6102 bits (135), Expect = 1.163e-8
Identity = 87/373 (23.32%), Postives = 151/373 (40.48%), Query Frame = 0

Query:  208 IWSSGTTGMPKGIQHS---FRMIADIINRGYGPLNAQTIFQSTC---FFHGGGFGFPISVLSGGQTACF--FPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLD--SVRYICPMGTNIPEDTYERLHGVFPALK-NVFNLYSLTEFGALITFSTTPRHLGS-VAPGTTVK--------IVDSDTQETLGANQVGEIMAKGTRIMMG---YLNRPKETEACLNS-DGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKS---EGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            +++SG+TG PKG+ HS   + +     ++         IF       +  G  +     +++GG +  F   PTY   +    LV      L  T  Y        S  AIR     E    L+ D  S+R +  +G  I  + +   + +    + ++ + +  TE G  I  +  P  L + + P   ++        ++D    E    N+ G +  K     M    Y +  +  E   ++  G+  TGD  +  ++G +   GR+ ++I      L   E+E+++ K   + E AV G P  I  E + A VV     EG    EK I  LV  ++  +  +     F   LP+   GKIMRR ++ +
Sbjct:  207 LYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPTYPDPSRYWKLVEE----LGITQFYT-------SPTAIRQLMKYEDSYVLNSDKSSLRTLGSVGEPINIEAWNWYNDLVGRNRCDIVDTWWQTETGG-ICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNEIKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGFA-VPEKFQFCEDLPKTRSGKIMRRILRKI 566          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 185.267 bits (469), Expect = 1.425e-50
Identity = 143/504 (28.37%), Postives = 240/504 (47.62%), Query Frame = 0

Query:   72 SEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGL-----VGNSKLI---------LDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            +E+E   RK A  L  +G + +D + + LPN+ E+ F  +G      + ++ +P   P  +  Q   S AK ++     + +V+    +  VK++ +   PE        F  +L + D+ +     +  D  +A+ +SSGTTG+PKG+  + + +   + +     NA     S              VL      C  P + + +    L     VG + LI         L+   +Y+           + +++   VD   DL SVR +      + ++  + +   FP  K +   Y +TE G ++     F+  P  + S A GT V     KIVD DT  +L  NQ GEI  +G +IM GYLN P+ T   ++ +G+LHTGD+G  DED  +F   RLKELIK++   + P EIE ++   P +S+ AV    D  + E+  A VV+S GS + E  + D +++ V  YKR++  V FV ++P++  GKI+R+ ++ 
Sbjct:   61 AEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEG----CLHF-SELTQSDEHEIPEVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSE------------DVL-----MCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVD-SYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAK-LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVK-RVFFVETVPKSPSGKILRKDLRA 539          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|126501|sp|P08659.1|LUCI_PHOPY (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 183.341 bits (464), Expect = 9.957e-50
Identity = 143/502 (28.49%), Postives = 235/502 (46.81%), Query Frame = 0

Query:   72 SEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGT-FLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEDPD--NHVYSFCQQL--AKVDDAQFVSDHLDIDQRMAIIW-SSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCF-----FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYIC----PMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            +E   MS + A+ ++  G      + +   N+ ++F PVLG  F+   V    D   + EL+    + S+   V      L ++    + L +++ +++ D   D      +Y+F         ++  FV +  D D+ +A+I  SSG+TG+PKG+    R      +    P+    I   T       FH G FG   ++   G   C F    +   E+      +L L +   Y+ +  +L        ++   +D + DL ++  I     P+   + E   +R H     L  +   Y LTE  + I  +      P  +G V P    K+VD DT +TLG NQ GE+  +G  IM GY+N P+ T A ++ DG+LH+GD+ ++DED   F   RLK LIK++   + P E+E I+ + P + +  V G PD  + EL  AVVV   G  + EK I+D V   V   K++RGGV+FV  +P+   GK+  R ++ +
Sbjct:   54 AEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNI-SQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHG-FGMFTTL---GYLICGFRVVLMYRFEE------ELFLRSLQDYKIQSALLVPTLFSFFAKSTLID-KYDLSNLHEIASGGAPLSKEVGEAVAKRFH-----LPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREI 538          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 175.252 bits (443), Expect = 6.006e-47
Identity = 144/512 (28.12%), Postives = 251/512 (49.02%), Query Frame = 0

Query:   72 SEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLM---VKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLV-----GNSKLI---------LDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNL-YSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            SE+E +SRK A  L  +G +   V+ L LPN  E+ F  +G  +   +V+  +P  KP  +  Q   + A+ +V     LA   +   DL    V ++ + D P++   H+      L + D+ Q  +  +  D  +A+ +SSGTTG+PKG+  + + +   + +     N         +FH           S     C  P + + +    L+     G + LI         L+   +Y+     +     + +++ + +  Q D+ SVR I     P+G  + +   ER    FP  K +F   Y +TE G ++     F+  P  + S + GT V     KI+D++T E+L  NQ GEI  +G  IM GY+N P+ T A ++ +G+LHTGD+ + D+D  +F   R+KE+IK++   + P E+E ++   P +++ AV  +    + E+  A VVKS  S ++E+ I + V + V  YK+I   V FV ++P++  GKI+R+ +++
Sbjct:   56 SEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIV----TLAAYVEKLADLQSHDVLVITIDDAPKEGCQHI----SVLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGEN------PNLYFH-----------SDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITK-SPIVSQYDVSSVRIIMSGAAPLGKELEDALRER----FP--KAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVVKS--SEISEQEIKEFVAKQVIFYKKIH-RVYFVDAIPKSPSGKILRKDLRS 532          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 174.481 bits (441), Expect = 1.336e-46
Identity = 145/498 (29.12%), Postives = 233/498 (46.79%), Query Frame = 0

Query:   72 SEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLG------DPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFR----MIADIINRGYGPLNAQTIFQSTC-FFHGGGFG-FPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQIN---EVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTE---FGALITFSTTPRHLGS---VAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 548
            +E+       A  L  +G     V+ L  PN+    FPV+    C  V+SLG      +P      +  QI +S           L ++  A + L   I+++ +   D    V    + + K      V + +D D    +++SSGTTGM KG+  S R    M+  I+NR +G  + +  F  T   FH  G   F   +L+ G T      + +             ++ A  +Y+   + L     I ++ +N   ++  + DL S+  +   G  + ++  E     +P +K +   Y LTE    GA        R  G+   ++     +IVD  T + LG  Q GE+  KG  IM GY +  + T + L+S+G+L TGDL + DEDG +F   RLKELIK++   + P E+E ++   P +++ AV   PD    +   A VV+  GS+++EKTI++ V + V  YKRIR  V FV S+P+N  GKI+R+
Sbjct:   61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNS--ILFPVV----CLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKL--PIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNR-FGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHE-----------MMSAIGKYQATSLPL--VPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVK-ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIR-KVAFVSSIPKNPSGKILRK 534          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 174.096 bits (440), Expect = 1.669e-46
Identity = 145/518 (27.99%), Postives = 243/518 (46.91%), Query Frame = 0

Query:   52 CNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVL-GTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLM-VKILVVRDCPEDPDNH--VYSFCQQLAK--VDDAQFVSDHLDIDQRMAIIW-SSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCF-----FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
              LG         G+D      +E  S    K L++ G      + L   N  E+F PV+ G F+  GV    +     EL+ + +  S+   V      L +V    + +  +K +V+ D   D   +  + +F ++        + F +  +D  +++A+I  SSG+TG+PKG+Q +        +    P+    +   T       FH G FG   ++   G   C F    L   ++      +  L     Y+   ++L       L++ +E+  + DL ++  I   G  + ++  E +   F  L  V   Y LTE  + I  +      P   G V P    K++D DT+++LG N+ GE+  KG  +M GY+N P+ T+  ++ +G+LHTGD+G+YDE+   F   RLK LIK++   + P E+E ++ + P + +  V G PDP++ EL  AVVV   G N+ EK ++D V   V + KR+RGGV FV  +P+   GKI  R ++ +
Sbjct:   37 AKLGAIAFTNAVTGVDYSYAEYLE-KSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELV-HSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPGTAVLTVVPFHHG-FGMFTTL---GYLICGFRVVMLTKFDE------ETFLKTLQDYKCTSVILVPTLFAILNK-SELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREI 540          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|148841518|sp|Q9S725.2|4CL2_ARATH (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumarate--CoA ligase isoform 2; Short=At4CL2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 173.326 bits (438), Expect = 3.522e-46
Identity = 136/497 (27.36%), Postives = 237/497 (47.69%), Query Frame = 0

Query:   78 SRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQ-----FVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQST----C---FFHGGGF-GFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            SRK A  L ++G +  DV+ + LPN+ E     L       + +  +P   P  +  Q   S AK +V     + +++    D +  ++V  D    P+N     C + +++  ++      + + +  +  +A+ +SSGTTG+PKG+  + + +   + +     N    F       C    FH        +  L  G T    P + +            L+L+   R +    M+     + +++  E + + DL SVR +      + ++  + +   FP  K +   Y +TE G ++     F+  P  + S A GT V     KI+D DT ++L  N+ GEI  +G +IM GYLN P  T + ++ DG+LHTGD+G  D+D  +F   RLKELIK++   + P E+E ++   P +++VAV    +  + E+  A VV+S+ SN++E  I   V++ V  YKRI   V F  S+P+   GKI+R+ ++ 
Sbjct:   73 SRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGV--LIVTTDSDAIPEN-----CLRFSELTQSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEI-----------TLLLEQIQRCKVTVAMVVPPIVLAIAKSPETE-KYDLSSVRMVKSGAAPLGKELEDAISAKFPNAK-LGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRI-NKVFFTDSIPKAPSGKILRKDLRA 548          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 173.326 bits (438), Expect = 4.170e-46
Identity = 139/504 (27.58%), Postives = 232/504 (46.03%), Query Frame = 0

Query:   72 SEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDI--DQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQST----CF---FHGGGF-GFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            +E+E  SR+ A  LR MG    DV+   L N  E+ F  LG        +  +P   P  +  Q   + A+ +V     + +VR+   +  V ++ V        +  +  C +  +V  A+ +    D+  D  +A+ +SSGTTG+PKG+  + R +   + +     N    F       C    FH        ++ L  G T      + L             ++D   ++            + +++   V  + DL S+R +     PMG ++ +    ++         +   Y +TE G ++     F+  P  + S + GT V     KIVD DT  +LG NQ GEI  +G +IM GYLN P+ T+  ++ DG+LHTGD+G  D+D  +F   RLKE+IK++   + P E+E ++   P + + AV    D ++ E+  A +V++EGS + E  I   V + V  YKRI   V F  S+P+N  GKI+R+ ++ 
Sbjct:   56 AEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFAAERGVPVVTV--------DGAFDGCVEFREVLAAEELDADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDL-----------GALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAE-DLASIRMVMSGAAPMGKDLQDAFMAKIPNAV-----LGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKRIN-KVFFTDSIPKNPSGKILRKDLRA 533          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|41688574|sp|Q27757.2|LUCI_PHOPE (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 172.94 bits (437), Expect = 5.401e-46
Identity = 148/542 (27.31%), Postives = 254/542 (46.86%), Query Frame = 0

Query:   30 EALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVS--LGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEDPDNH--VYSFCQQLAKV--DDAQFVSDHLDIDQRMAII-WSSGTTGMPKGIQHSFRMIADIINRGYGP-----LNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEF--GALITFST--TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMY 554
            E +F  + +  + +G    L N +     L E+ + L CR+           A+  +  G +  D + +   N  ++F P++ + L  G+++  + D  ++ EL+ + +   + + + C      +V      L  V+ +++ D  ED   +  + +F  Q + +  D  +F  +  + D ++A++ +SSGTTG+ KG+  + + I    +    P     +N  T   +   FH G FG   ++   G   C F    +   E+      KL L +   Y+ E  +L         +   V+ + DL  ++ I   G  + ++  E +   F  L  V   Y LTE     LIT  T   P   G + P   VK+VD  T + LG N+ GE+  KG  IM  Y N  + T+A +N DG+L +GD+ +YD DG  +   RLK LIK++   + P EIE I+ + P + +  V G PD  + EL  A VV   G  +NE+ + + V+  V   K +RGGV F+  +P+ S GKI R+ ++ M+
Sbjct:   23 EQMFYALSRYADISGCIA-LTNAHTKENVLYEEFLKLSCRL-----------AESFKKYGLKQNDTIAVCSENGLQFFLPLIAS-LYLGIIAAPVSDKYIERELI-HSLGIVKPRIIFCSKNTFQKVLNVKSKLKYVETIIILDLNEDLGGYQCLNNFISQNSDINLDVKKFKPNSFNRDDQVALVMFSSGTTGVSKGVMLTHKNIVARFSHCKDPTFGNAINPTTAILTVIPFHHG-FGMTTTL---GYFTCGFRVALMHTFEE------KLFLQSLQDYKVESTLLVPTLMAFFPKSALVE-KYDLSHLKEIASGGAPLSKEIGEMVKKRF-KLNFVRQGYGLTETTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGKILGPNETGELYFKGDMIMKSYYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAPAEIEGILLQHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTAKWLRGGVKFLDEIPKGSTGKIDRKVLRQMF 538          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 171.785 bits (434), Expect = 1.494e-45
Identity = 140/487 (28.75%), Postives = 232/487 (47.64%), Query Frame = 0

Query:   85 LRSMGFEFQDVLHLALPNTTEYFFPVL-GTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPED------PDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIW-SSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCF-----FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            L++ G      + L   N  E+F PVL G F+  GV    +     EL+ + +  S+   V      L +V    + +  +K +V+ D   D       DN +     Q  K   + F +  ++  +++A+I  SSG+TG+PKG+Q +        +    P+    +   T       FH G FG   ++   G   C F    L   ++      +  L     Y+   ++L       L++   +D + DL ++  I   G  + ++  E +   F  L  V   Y LTE  + I  +      P   G V P    K++D DT++TLG N+ GE+  KG  +M GY++ P+ T   ++ +G+LHTGD+G+YDE+   F   RLK LIK++   + P E+E ++ + P + +  V G PDPI+ EL  AVVV  +G ++ EK ++D V   V + KR+RGGV FV  +P+   GKI  + ++ +
Sbjct:   69 LKNYGLVVDGRIALCSENCEEFFIPVLAGLFIGVGVAPTNEIYTLRELV-HSLGISKPTIVFSSKKGLDKVITVQKTVTAIKTIVILDSKVDYRGYQSMDNFIKKNTPQGFK--GSSFKTVEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRFSHARDPIYGNQVSPGTAILTVVPFHHG-FGMFTTL---GYLTCGFRIVMLTKFDE------ETFLKTLQDYKCSSVILVPTLFAILNRSELLD-KYDLSNLVEIASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGKAIREI 540          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|61213879|sp|Q26304.1|LUCI_LUCMI (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 171.014 bits (432), Expect = 2.832e-45
Identity = 144/525 (27.43%), Postives = 244/525 (46.48%), Query Frame = 0

Query:   52 CNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLM--------VKILVVRDCPEDPDNH--VYSFCQQLAKV--DDAQFVS-DHLDIDQRMAIIW-SSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCF-----FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
              LG    +    G+D     E   ++ + A+ +++ G + ++ + L   N  E+F PVL        V+       P    Y + E      +  P  +   RK    ++        +K +V+ D   +   H  + +F ++  ++    + FV  D  +  Q +A++  SSG+TG+PKG++ +        +    P+    +   T       FH G FG   ++   G  AC +    L   ++      +L L     Y+   ++L       L++   +D + DL ++  I   G  + ++  E +   F  L  V   Y LTE  +    +      P   G V P   VK++D DT++TLG N+ GEI  KG  +M+GY N P+ T   ++ +G+LHTGD+G+YDED   F   RLK LIK++   + P E+E ++ + P + +  V G PDP + EL  AVVV  +G  + EK I+D V   V ++KR+RGGV FV  +P+   GKI  + ++ +
Sbjct:   36 AKLGAIAFSNALTGVDISY-QEYFDITCRLAEAMKNFGMKPEEHIALCSENCEEFFIPVLAGLYIGVAVA-------PTNEIYTLRELNHSLGIAQPTIVFSSRKGLPKVLEVQKTVTCIKKIVILDSKVNFGGHDCMETFIKKHVELGFQPSSFVPIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRFSHAKDPIYGNQVSPGTAILTVVPFHHG-FGMFTTL---GYFACGYRVVMLTKFDE------ELFLRTLQDYKCTSVILVPTLFAILNKSELID-KFDLSNLTEIASGGAPLAKEVGEAVARRF-NLPGVRQGYGLTETTSAFIITPEGDDKPGASGKVVPLFKVKVIDLDTKKTLGVNRRGEICVKGPSLMLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKVIREI 540          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 246.514 bits (628), Expect = 6.502e-71
Identity = 177/566 (31.27%), Postives = 276/566 (48.76%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMV-----KILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDI-----DQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTI-FQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQE-LVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMY 554
            MS I+DKVLYSK P       D  +E ++  L   +Q  +   GD  ++++C        E+ V +   +G      S K A  L  +GF+  D LH A     ++++P+ G +LC GVVS+GDP L    ++ QI ++ AK +VC   + ++  +A + L+       ILV+   P  P     S  + L + D      D   +      + + I W+SGTTG PKG+  S   +   + +   P   +TI   S  FFH G F   +  L GG    F   +      Q      + I     +Y+P   M G++  ++L+  +    + DL SV  I P G  I +    R+   FP LK V+  Y  TE G  ++ S T  ++G++  G    I D  T E LG  +VGEIMAK   +M+ YLNR  E+   L+ +GF H GDLG+YDE+GI+ +  R+KE+IK  N  + P E E+ +  +PGV E  V+   +  +    +   V     S +N + IL+L  + V+  +RI G +  + ++P N  GK +RR V+ M+
Sbjct:    1 MSEIRDKVLYSKEP----YTLDPGQERIIFKLLDLIQAELRETGDRDWILDCT------TENSVRISHILGR-----SIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPIFGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVLDLEPWQPIPEGTSSFRSLYE-DQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQSKLP--EKTIGLASNTFFHAGSF---LLSLDGGIRNKFSIHFI-----QPKHTTVESIFRFIEKYKPMFFMCGANDFVQLASSDLA--KFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGG-VSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVWQSMNKETDNYYIHGAVTLQASSGLNPEKILELANKDVEVKRRIDGTIHIINNMPHNPQGKKLRRTVRQMF 537          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325261085|ref|XP_023330441.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 239.195 bits (609), Expect = 3.373e-68
Identity = 168/564 (29.79%), Postives = 277/564 (49.11%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLM-----VKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDI--DQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYG-PLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH---LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPIS--QELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVK 551
            M++IKDK++YS +       P+   +  +E L  ++ Q ++  GD  +L++   G             R  S++EP+SR+ A  L S+GF   DVL     +  ++++PV G +LC G VSLGDP+L    ++ Q+ E++AK +V     L +  K  ++L      VK  ++    +D         Q L +V + Q +   LD   D+   I+WSSG++G PKGI HS +   +++N  +G  L  +TI  S   FH   F F +++     T C+         E+   G +  +L    + +PE +  G    I++S  N      +L+ V+ I P+G  +     E++  +  +   +  +Y  TE   +            LGS+A G  + I D +T E L   + G+IM K   +MM YLNR KET    +SDGF  TGD+G+YD  G +FF  R++E++K +N    P EIE ++ K P + E  V+G+ DP +   ++   +V K       +  I  LV  ++   ++I G + FV  +P N  GK +R  +K
Sbjct:    1 MNVIKDKIIYSTLEPK----PEQPNQPYIERLLSEINQELDDTGDRDWLVDTETG-----------SSRTISQIEPISRRVASALLSLGFGPGDVLQTGYSSCLDFYWPVFGAWLCGGTVSLGDPNLSETNIKKQLEETKAKVLVSSIDYLDKYYKVIQELKLQGKNVKYFILDAGEQDTLPLGVESFQTLMRVQETQKMPLLLDFTPDRESIIMWSSGSSGTPKGILHSQK---NLLNLYFGHDLKPKTIISSALMFHISSFTFNLTLGILKGTICYI------MKEKCFSGEN--LLKVAEKIQPEHVFCGVSQYIQVS--NTKTNGYNLNGVKNITPLGGAVSPGCSEKVLSLLGSQATLVEMYGSTEVMFVSKHEVKDIEFGLLGSLAAGVEICIQDINTGERLDPGKEGKIMVKTNTMMMRYLNREKETNEFFDSDGFGFTGDIGYYDNFGKIFFSYRMREVLKVDNYWFGPAEIENLLEKEPTIDEACVWGEYDPNTGNDQVNLGIVQKQNQKEWTKAKICSLVDANLPCTRKITGKIYFVEKIPHNPQGKKLRGEMK 536          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325319021|ref|XP_023341279.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 232.261 bits (591), Expect = 1.381e-65
Identity = 164/565 (29.03%), Postives = 282/565 (49.91%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQ---VRKATR--DLMVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDI--DQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISV-LSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGAL-ITFSTTPRH--LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPD--PISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKT 552
            M+++KDKV+YS +       P    +  +E L  +V Q IE  GD  ++++   G                S++EP+SR+ A  L ++GF   DVL     +  ++++P  G +LC G VSL DP+L    ++ QI +++AK +VC    L +   +++  R  D+   + V+ +   +    V SF Q     D     ++ L+   D    ++WSSG+TG PKGI HS R +  I      P + ++I  S   FH GGF   +S+ L GG T+ F         E+   G +   +    +++PE + +G    I++S+   VD   +L  V+ I P+G  +  +  E++  +      +  +Y  TE   L    +  P+   LG++  G  + I D  T E LG  + G++M +   +MM YLNR +E     NSDGF + GD+G+YDE+G +FF  R++E++K +N    P EIE ++  L  ++E  V+G+ D    + ++   +V+++      E  I + +T ++   +RI G +  +  +P N  GK +RR +K 
Sbjct:    1 MNIVKDKVIYSPL----PPRPSPPYQPYIERLLAEVSQEIEETGDRDWIVDTETG-----------QSHTISKIEPVSRRVAGALHNLGFGPGDVLQTGYSSCLDFYWPAFGAWLCGGAVSLADPNLSSTAIKQQIKDTKAKIIVCSKQYLDKYCAIKQELREEDVHFNLSVLDEEDGNLPADVKSF-QSFLNFDGDISHNEKLEYIPDSVTMVLWSSGSTGNPKGILHSHRTLQTI--SLAKPSSPKSILVSAIMFHIGGFTLNLSIALFGGTTSYFIK-------EKCFSGENWFKI--AEKFQPEHVYIGVSQFIQVSKAKNVDR--NLSGVKMITPLGGAVSPECCEKVLSMVGDQAKIIEMYGSTEIVFLAFKLNKHPKFGFLGTLGSGAEMYIQDIKTGEKLGPRKEGKMMVRTRSMMMNYLNREEENREFFNSDGFGYVGDVGYYDEEGNIFFCYRMREVLKVDNYWFGPEEIENLMEALDDINEACVWGEYDMNTGNDQVSLGIVLQANKEEWTETRINEYITSNLPITRRITGKIYLLDRIPHNPQGKKLRREMKN 536          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1351476638|ref|XP_013404248.2| (4-coumarate--CoA ligase 1 isoform X2 [Lingula anatina])

HSP 1 Score: 219.164 bits (557), Expect = 1.280e-60
Identity = 179/575 (31.13%), Postives = 276/575 (48.00%), Query Frame = 0

Query:    8 VLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL--MVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQR--MAII-WSSGTTGMPKGIQHS-FRMIADIINRGYGPLNAQTIFQSTCF------FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHA----IRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALI----TFSTTPRHLGS---VAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMYS 555
            V+ SK+P     IP+ V      +L + V QN E  GD   L+N           E G        ++ + R  + L ++ G    DV ++  PN  EY F +LG     GVV+  +P    E + YQ+  ++A F++  P N+++V+ A R++  +  + VV D P        +F   +   DD       +  + R  +AI+  SSGTTGMPK +  + + ++A+++      +  +      C+      FH  G     SVL  G   C             +V   K  LD+  +   E      H      I L++   VD + DL  VR I     P+G N      +RL G+     ++   Y LTE   ++      +  PR  GS   + P T  K+VD DT++ LGANQ GE+  +G ++M GY N P+ T  C++ DG+ HTGD+GHYDE+G  +   RLKELIK++   + P E+E +I   P V++VAV G PD I+ EL  A V+         + I D V +H+  +KR+RGGV FV  +P++  GKI+RR ++   S
Sbjct:    7 VVRSKLPSVE--IPENV------SLAEFVLQNAEKYGDLVALVN----------GETGQQFTYRELLQNVRRVGSGLFKA-GLRKNDVFYIYCPNVPEYAFGLLGALAIGGVVTTVNPLYTEEELEYQLRSTKATFIMTVPENVSKVKDAAREIGHLKVVYVVGDAPAG------TFPFSVLLNDDGIAFPSFVPFNTREDVAILPSSSGTTGMPKPVMLTHYNLVANLMQIDNPYIQTRVTPGEDCYVCVLPVFHIFGL---TSVLFKGLRLCL-----------KVVTVRKFHLDSYLKIVTENKGSVLHCVPPIVIHLAKNPLVD-KYDLSCVRDILSGAAPLGKNTEIAIQKRL-GI-----SIGQGYGLTETSPVVLAPPRVNCFPRKSGSSGILVPNTESKVVDPDTKQVLGANQDGELWFRGPQVMKGYFNLPQATAECIDKDGWFHTGDIGHYDEEGYFYVVDRLKELIKYKGFQVAPAELEHLIHTHPAVADVAVIGVPDEIAGELPKAYVILKHDVTATAQDISDFVAEHLAPHKRLRGGVEFVTEIPKSPSGKILRRELRKRAS 535          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1059227323|ref|XP_017759168.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Eufriesea mexicana] >gi|1059227325|ref|XP_017759169.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Eufriesea mexicana])

HSP 1 Score: 218.394 bits (555), Expect = 2.118e-60
Identity = 166/556 (29.86%), Postives = 268/556 (48.20%), Query Frame = 0

Query:   15 EDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRY--QILESRAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEDPDNHVYSFCQQLAK----VDDAQFVSDHLD-IDQRMA-IIWSSGTTGMPKGIQHSFRMIADIINRGYGP--LNAQTIFQSTCF---FHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            +DN I  D V+E    +L Q +   + TN D    I+         E +    CR   E+   S   + +L++ G   +D +  A  N   Y   +  T       +  +P       R+  +I + R  FV      +     +T +   K++ + D  +  + ++ +  + L K     D   F    +D I +R A I+ SSGTTG PKG+  S R +   I+   GP  ++ +   ++  F   FHG  FG     +S G  AC    + LE           ++L +  R+R   + L     + L++   V+ + D  SVR I     P+  N+  +   R      +++N + +  LT    +   +     +G + PG   K+VD+ T +TLGA Q GEI   G ++M+GY   PK T   ++ + +LHTGDLG++ EDG ++  GR+KELIK++   + P EIE II   PGV +VAV GKPD +S E+  A+VV+  G NV+ K I+D   +++   K +RGGVIF+ SLP+   GKI+R+ + +M
Sbjct:    4 QDNIIYGDPVEEVPNVSLGQHLFDKLRTNADHLAQIDI--------ESDKHYTCR---EILEKSVILSTVLKNYGINIEDRISTASENHPNYILSICSTLFVGATFAPLNPAYTEREFRHMLEIYQPRVIFVSQRTEKIIAKVVSTVNWSTKLIQLED--QSLNRNIMTLKEILEKNRNIPDPYTFTPVQIDDISKRAAAILCSSGTTGFPKGVMLSHRSLLIFIHTTKGPKLMDMRQGDRTLIFLPLFHGYAFGLINQTISSGGIACIMRNFNLE-----------MLLKSVERFRISHIPLVPPILVMLAKHPMVE-KYDFSSVREITSGAAPLPQNVANEVKRRTK--VGSIRNGYGMTELTIVSNISDKTCLDDTIGPIMPGFKCKVVDTKTGKTLGAGQSGEICFMGDQVMLGYYKNPKTTAETIDRENWLHTGDLGYFKEDGCLYITGRIKELIKYKGFQVSPSEIEAIILTHPGVKDVAVLGKPDELSGEVPMALVVRQPGKNVSVKEIVDFANENLSPQKWLRGGVIFMESLPKTPSGKILRKQLLSM 532          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1351476634|ref|XP_013404245.2| (4-coumarate--CoA ligase 1 isoform X1 [Lingula anatina])

HSP 1 Score: 218.009 bits (554), Expect = 3.289e-60
Identity = 177/572 (30.94%), Postives = 276/572 (48.25%), Query Frame = 0

Query:    8 VLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL--MVKILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQR--MAII-WSSGTTGMPKGIQHS-FRMIADIINRGYGPLNAQTIFQSTCFFHGGGFG-FPISVLSGGQTACFFPTYALENTEQG--LVGNSKL----ILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALI----TFSTTPRHLGS---VAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTMYS 555
            V+ SK+P     IP+ V      +L + V QN E  GD   L+N   G +               E+    R+    L   G    DV ++  PN  EY F +LG     GVV+  +P    E + YQ+  ++A F++  P N+++V+ A R++  +  + VV D P        +F   +   DD       +  + R  +AI+  SSGTTGMPK +  + + ++A+++      ++   I+ S         G  P   + G     F      +  EQG  +V   +      ++ T R+R   +       I L++   VD + DL  VR I     P+G N      +RL G+     ++   Y LTE   ++      +  PR  GS   + P T  K+VD DT++ LGANQ GE+  +G ++M GY N P+ T  C++ DG+ HTGD+GHYDE+G  +   RLKELIK++   + P E+E +I   P V++VAV G PD I+ EL  A V+         + I D V +H+  +KR+RGGV FV  +P++  GKI+RR ++   S
Sbjct:    7 VVRSKLPSVE--IPENV------SLAEFVLQNAEKYGDLVALVNGETGQQFTYR-----------ELLQNVRRVGSGLFKAGLRKNDVFYIYCPNVPEYAFGLLGALAIGGVVTTVNPLYTEEELEYQLRSTKATFIMTVPENVSKVKDAAREIGHLKVVYVVGDAPAG------TFPFSVLLNDDGIAFPSFVPFNTREDVAILPSSSGTTGMPKPVMLTHYNLVANLMQ-----IDDSGIYASVTPGKDSFIGALPFYHIYGMVQIVF------KGIEQGARVVTMRRYHFAEYVELTSRFRGSVLHCVPPIVIHLAKNPLVD-KYDLSCVRDILSGAAPLGKNTEIAIQKRL-GI-----SIGQGYGLTETSPVVLAPPRVNCFPRKSGSSGILVPNTESKVVDPDTKQVLGANQDGELWFRGPQVMKGYFNLPQATAECIDKDGWFHTGDIGHYDEEGYFYVVDRLKELIKYKGFQVAPAELEHLIHTHPAVADVAVIGVPDEIAGELPKAYVILKHDVTATAQDISDFVAEHLAPHKRLRGGVEFVTEIPKSPSGKILRRELRKRAS 535          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325317676|ref|XP_023340582.1| (4-coumarate--CoA ligase-like 7 [Eurytemora affinis])

HSP 1 Score: 215.312 bits (547), Expect = 3.605e-59
Identity = 166/569 (29.17%), Postives = 277/569 (48.68%), Query Frame = 0

Query:    1 MSLIKDKVLYSKM-PEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLM-----VKILVVRDCPED--PDNHVYSFCQQLAKVDDAQFVSDH-LDIDQRMAIIWSSGTTGMPKGIQHSFRMIADI-INRGYGPLNAQTIFQSTCFFHGGGFGFPI--SVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQIN-EVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH---LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPIS--QELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVK 551
            M++IKDKVLYS + P+     P+   +S ++ +   V+  ++  GD  +L++         E E+ +     S++EP SR+ A  L S GF   DVL +   +  ++++PV G +LC GV S+GDP+L+   ++ QIL++RAK VVC    L +  K   +L      +K+ V+    E   PD  V  F   L   ++  FV  H  D +    IIWSSG+TG+PKGI  S   + +I + + Y P   + I  S   FH GGF   +  S+  G    C+F      + E        +   +  +++ E    G     RLS +  +V P   LD ++ I P+G ++       L  +     ++  +Y  +E   + ++S        LG + PG  +   D +T   L   + G+IM K   +M+ YLNR KET+   +SDGF + GD+G+YD  G +++  R++E++K       P EIE ++ K+  + E  V+G  DP +   ++   +V++          I   V  ++ + + I G +  V SLP N  GK +R  ++
Sbjct:    1 MNIIKDKVLYSPLGPKAKP--PN---KSFIDRVLSHVKHELKETGDRDWLVD--------TETELTISI---SQIEPTSRRVAAALYSHGFRADDVLQMGYSSCLDFYWPVFGAWLCGGVASMGDPNLEEMAIKQQILDTRAKVVVCSKQYLDKYVKVLLELRKEECNLKLFVLDAGKEVILPDG-VEHFQNLLEFKENNDFVGLHSYDPNSEALIIWSSGSTGVPKGIVFSQLGLNNISLGKQYQP---KKILLSLIMFHIGGFSVSVLLSIFEGA--VCYFIKKECFSEE--------VWFKSVDKFQLEHAFCGISQYTRLSNVKIDVKP---LDGLKVITPLGGSVTSQGTLNLLNLVGHHVSIIEMYGSSEVTFISSYSNKEFKSCVLGVLDPGVQMYFEDMETGVKLEPGKEGKIMIKNESMMLYYLNREKETKEFYSSDGFGYMGDVGYYDATGAIYYSYRMREVLKVSGCWFGPAEIENVLEKVNEIDEAFVWGDYDPNTGNDKVNVGIVLRKGIVEWTATNIKKYVEDNLPESRHITGKLYIVESLPHNPAGKKLRGEMR 536          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325273277|ref|XP_023321279.1| (4-coumarate--CoA ligase-like 7 [Eurytemora affinis])

HSP 1 Score: 213.772 bits (543), Expect = 1.089e-58
Identity = 157/571 (27.50%), Postives = 274/571 (47.99%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMVK---ILVVRDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDIDQ-----------RMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTCFFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYICPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGAL--ITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPI--SQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
            M+LIKDK+LYS   E    + D  K  +   L   V Q ++  GD  ++++           E G   ++ S + P+S + A     +GF+  DV+        ++++P+ G ++C GVVSLGDP L P+L+RYQIL+ ++K +VC   +  +  K    L V+   +L V D  E   N V   C+   ++    F  D LD              R+ ++WS+GTTG PKGI H  + + ++    Y          +   +H  GF   +S    G+T  +F        E+   G++   L+A  +Y+PE  +        ++  N   P  +L  ++ + P+G ++   T ER+  +     +   +Y  TE G +    +S    ++G + PG  +   D  T   LG +Q G+ M K   +M+GYL +    E+  +SDGF ++GD+G+YDE G ++F  R+K+++K EN    P EIE I+   P + +  V+G  D    S ++   V+ ++  ++  E  +  L+ + +   + I G V  +  +P +  GK +RR +++ 
Sbjct:    1 MNLIKDKILYSGEKE----VRDYGKYFIYRVL-DDVHQELDETGDRDWMVDT----------EDGGSTKL-SSIYPISIRIASAFSKLGFKAGDVIQTGYSTCLDFYWPIFGAWMCGGVVSLGDPSLNPDLIRYQILDLKSKIIVCSSEHALKYSKIVASLTVEEKPMLFVLDVLE---NKVPIGCRSFTEL----FEDDGLDAPSYESLPPYNPLDRLVVLWSAGTTGNPKGIVHCHQTLQNV---AYLKNICNKSLITNVGYHMAGFLHHLSSGILGRTISYFV------KERLFSGDT--YLNALEKYQPEYFVCSVSSFTSIA--NAKQPFRNLPKLKIVTPLGGSLSPGTVERVMKILGEHVSFLKIYGGTEMGLIGKCVYSKDYDNIGQLGPGVQLYCADPVTGSKLGPHQEGKFMVKTRSMMLGYLKKSDNDES-YDSDGFAYSGDMGYYDEHGNIYFKCRMKDVLKVENFWFGPGEIENILLNEPEILDAVVWGVYDNTTGSDKVHLGVIFENTENSWTEADVKSLIAKKLPRTRHITGQVHILTKIPYSVQGKKLRREMQSF 534          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1126184687|ref|XP_019624977.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Branchiostoma belcheri])

HSP 1 Score: 214.542 bits (545), Expect = 2.128e-58
Identity = 176/575 (30.61%), Postives = 283/575 (49.22%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCNLGLRGLAEDEVGLDCRMGSEMEPMSRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDL---MVKILVV-RDCPEDPDNHVYSFCQQLAKVDDAQFVSDHLDID--QRMAII-WSSGTTGMPKGIQHSFRMIA----DIINRGYGPLNAQ--TIFQSTCFFHGGGFGFPISV-LSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVR-YIC---PMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVKTM 553
             S +++ +L S+ P+ N  IP        E L Q V Q+ +  G+   + N   G+ G +   +        +++ + R+    L  +GF+  DVL +  PN  EY     G     GVV+  +P   P+ + YQ+  S A++V+  P ++A+  KA +     + +I V+  D PE       +F   LA  DD     +++ ++  + +A++ +SSGTTG PKG+  +   I      IIN+G    N Q   +     FFH  G    +S  L  G      P +  E           L L     Y+   +M+    A+ LSQ   VD Q DL  V+  +C   PMG N+     ++L       +++   Y LTE   +          P  +G   P T VK++ ++T + LG  + GEI  +G ++M GYLN P+ T  C++SDG+ HTGD+GHYD+ G  +   RLKELIK++   + P ++E ++   P V +VAV G PD  + E+  A +VK +  +V EK I+D V   V  +K++RGGV FV  + +++ GKI+RR ++ M
Sbjct:   68 FSTVENGILKSRFPDVN--IPG------NETLTQYVTQHFDDYGNDVAITN---GVTGESYTYL--------QLQDLIRRFGSALTRLGFKQHDVLAIFSPNVPEYAIAFFGASSVGGVVTTANPTYTPDELAYQLQHSNAQYVITIP-DVAETVKAAKSKCPSVKEIFVIGSDVPE-----CRNFSDLLA--DDGSAFPENVSVNTAEDVAVLPYSSGTTGRPKGVVLTNNNIVANLRQIINKGMLEFNRQEDCLIAQLPFFHIYGMVAVLSCCLRQGVRFVTIPRFEPE-----------LYLRVIQDYKVNRVMMVPPIALFLSQHPMVD-QYDLSRVKDLMCAAAPMGINLTMALRDKLKP-----QSLRQGYGLTETSPVTHLCVEDEFAPGSVGITLPNTEVKVIHTETGQALGEGEDGEICVRGPQVMKGYLNNPEATAGCIDSDGWFHTGDIGHYDDKGYFYIVDRLKELIKYKGLQVAPADLEAVLLGHPSVQDVAVIGVPDERAGEVPKAFIVK-KSDDVTEKEIVDYVAGKVAPFKKLRGGVEFVNEISKSASGKILRRTLRDM 597          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|746860456|ref|XP_011061069.1| (PREDICTED: luciferin 4-monooxygenase-like [Acromyrmex echinatior] >gi|332021126|gb|EGI61513.1| Luciferin 4-monooxygenase [Acromyrmex echinatior])

HSP 1 Score: 209.92 bits (533), Expect = 3.067e-57
Identity = 158/496 (31.85%), Postives = 243/496 (48.99%), Query Frame = 0

Query:   78 SRKTAKLLRSMGFEFQDVLHLALPNTTEYFFPVLGTFLCQGVVSLGDPDLKPELMRYQILESRAKFVVCHPGNLAQVRKATRDLMV--KILVVRDCPEDPDNHVYSFCQQLAKVDDA---QFVSDHLDIDQRMAIIWSSGTTGMPKGIQHSFRMIADIINRGYGPLNAQTIFQSTC------FFHGGGFGFPISVLSGGQTACFFPTYALENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIRLSQINEVDPQLDLDSVRYI----CPMGTNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR----HLGSVAPGTTVKIV---DSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLNSDGFLHTGDLGHYDEDGIMFFDGRLKELIKFENKHLYPMEIEEIIRKLPGVSEVAVFGKPDPISQELVTAVVVKSEGSNVNEKTILDLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHVK 551
            SR+ A  L+  G +  D + +   N  E+  PV   F    +    +P       ++ +  S+ K++     +   +RK  R+L    KIL++ D   +              +DDA   Q  S +LD D   AI+ SSGTTG+PKG+  + + I  +I R Y  +N  TI ++        FFH   F F I  +  G  +  F  +  E           L L    +Y+ E M +     + L++   VD + DL  V+ I     P+   I +   +RL+     + +V   Y LTE    +  S   +     +G V PGT VK++   + +T + LG N  GE+  KG  IM GY N  + T A ++ DG+LHTGD+G+YDEDG  +   R+KELIK++   + P E+E I+   PGV + AV G P+  + EL  A +VK E SN+ EK I+  V + V + KR+RGG+ FV S+P+   GKI+RR ++
Sbjct:   57 SRRLAVYLQREGLKVNDTIAVCSENNLEFCIPVCAAFYLGAIACPLNPLYSEREFKHALSISKPKYIFISTVSFNGIRKIFRELHWSPKILMLTDGNNNVSWASMYKVMSNVSIDDANALQPASVNLD-DHVTAILCSSGTTGLPKGVMLTDKNITTVI-RMY--MNTNTIPENAVSLSLLPFFHAYSFVFMILTILRGNCSIIFSHFEEE-----------LFLQYIEKYKIEYMPMVPSLMVFLAKHPLVD-KYDLSCVKTIWSGAAPLSKEIQQAVAKRLNM---NIIDVKQGYGLTETTLAVLRSPDGKGKLGSVGVVVPGTLVKVIPIGEYETDKALGPNCEGELCFKGDLIMKGYYNDEESTRATIDKDGWLHTGDVGYYDEDGYFYIVDRIKELIKYKGYQVPPAELEAILLTFPGVQDAAVIGIPNDKTGELPMAFIVKEENSNICEKDIIQYVNERVSNPKRLRGGIRFVDSIPKTPSGKILRRVLR 533