BLAST of threonyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000010574 (supercontig:LSalAtl2s:LSalAtl2s702:126834:130647:1 gene:EMLSAG00000010574 transcript:EMLSAT00000010574 description:"maker-LSalAtl2s702-augustus-gene-1.17")

HSP 1 Score: 314.694 bits (805), Expect = 5.000e-100
Identity = 168/421 (39.90%), Postives = 255/421 (60.57%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEG-----------------------------KSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLL-EGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELF 391
             D+A+ IS+GLA N + A+V+G+V+D +R + +   ++L+ + D EG                             K+ FWHSSAH++ EA+E +Y G     GPP+ENGFYYD+ + +H +S +DF  ++     + ++K  FER+ M K D +  FK   +++KL +L E ++    T Y+ G   DLCRGPH+ +TG IK+  IT  +  +++G+   + L RIYGI+FP    +KE+    EEA KRDHRKLG+E DLF F + +  G   + PKGA +  +L +F+K+   + G++ V++P+I +  L++TSGHW+ Y E+ F   +   E E+F LKPMNCP HC IF    RS++DLP R+++FG ++R E SG L GL RVR F QDDAHIFC  +Q++DE K   D+   ++
Sbjct:  141 FDIAMGISKGLADNCVIARVDGQVFDLDRVLEKSCRLELIKFDDEEGNLDFFNLFIYYFNFQYLIFEMIHLFMIICKAVFWHSSAHILGEAMERVYGG-HLCYGPPIENGFYYDM-SSDHKVSDSDFKVLDNMTKSIVKEKQPFERLEMKKEDLLEMFKY--NKFKLRILNERVKTPTTTVYRCGPLIDLCRGPHVRHTGKIKSMAITKNSATYWEGNSEAESLQRIYGISFPDNKLMKEWKLFQEEAAKRDHRKLGREQDLFFFHE-LSPGSCFFQPKGAHIYNKLIDFIKEEYWERGFKEVISPNIYNANLWKTSGHWDHYAENMF---RFEIEKEKFGLKPMNCPGHCLIFDHGVRSWRDLPIRMSDFGVLHRNELSGALTGLLRVRRFQQDDAHIFCMPNQIKDEMKGALDFLKAVY 553          

BLAST of threonyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000001588 (supercontig:LSalAtl2s:LSalAtl2s1278:38379:43347:-1 gene:EMLSAG00000001588 transcript:EMLSAT00000001588 description:"maker-LSalAtl2s1278-snap-gene-0.26")

HSP 1 Score: 76.2554 bits (186), Expect = 9.709e-16
Identity = 58/226 (25.66%), Postives = 103/226 (45.58%), Query Frame = 0

Query:    2 DVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEG----KSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENG-FYYDVDT--GEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDG-----NITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMV 215
            + A  I E L      A VN ++WD NRP+ ED  ++ + ++ TE        FW S + ++   LE L+   +++I   +++G F YDVD    + + S  +     A M   +  +  FER+ +  + A+  F  +D++YK   +  +        ++T Y+ G   D+  GP I N+GF+   +  +V      G     ++ R  G+  PK   L  Y  + 
Sbjct:   83 NAAQHIHETLIERSALATVNDKIWDMNRPLEEDCVLKFIHFQQTEDPYLVNKAFWRSCSFMLGATLETLFQD-QYYIELHIQSGSFVYDVDLKFKDWAPSKEELMSFSAYMHRFSESELPFERLTVDASVALKMF--EDNQYKYKQIPSIAANSNSGHSVTLYRMGEHIDISGGPMIGNSGFL--GRRCSVHPIVHDG----IEMYRFQGVALPKGIFLDHYAMLT 299          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|123354610|sp|Q11UK3.1|SYT_CYTH3 (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 298/407 (73.22%), Postives = 349/407 (85.75%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            M +ALSISEGLARNVL+AKVNGE+WD++R I  D++VQLLTW DTEGKSTFWHSSAHL+AEALE LYPG KF IGP +E GFYYDVD G+ + S +DF  IE K++ELA+QKN + R  +SK DA++YF +K D YKLDLLEGL DG+ITFYKQG FTDLCRGPHIP+TGFIKA K+ +VAGA+++GD+ +KQLTRIYG+TFPKQ ELK+YLT++EEAKKRDHRKLG+EL+LFAFS+KVG GLPLWLPKGA+LRERLENFLKKAQV+AGYQ VVTPHIG+K+LY TSGH++KYG+DSFQPI TP EGE+F+LKPMNCPHHCEI+KT+PRSYKDLP R AEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQV++EF K  D    +F  L   D    +  RD
Sbjct:   21 MGIALSISEGLARNVLAAKVNGEIWDASRAITSDSTVQLLTWNDTEGKSTFWHSSAHLLAEALEALYPGTKFGIGPAIETGFYYDVDFGDRAFSQDDFENIEKKVLELAKQKNAYIRKEISKKDAVAYFTDKQDPYKLDLLEGLSDGSITFYKQGAFTDLCRGPHIPDTGFIKAVKLMSVAGAYWRGDEKSKQLTRIYGVTFPKQQELKDYLTILEEAKKRDHRKLGKELELFAFSEKVGMGLPLWLPKGALLRERLENFLKKAQVRAGYQPVVTPHIGNKQLYVTSGHFDKYGKDSFQPIFTPHEGEQFLLKPMNCPHHCEIYKTKPRSYKDLPIRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRPDQVKEEFLKVIDLVLYVFKVLGFNDYTAQISLRD 427          

HSP 2 Score: 70.4774 bits (171), Expect = 8.641e-12
Identity = 30/55 (54.55%), Postives = 44/55 (80.00%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +K+ DYA +++  L  +DIRG++D+RDEKIG+KIRD E+ K+P ML++GEKE  E
Sbjct:  556 EKFNDYAQDVYDRLLVKDIRGFIDNRDEKIGKKIRDTEVKKVPFMLVIGEKEVNE 610          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|166225472|sp|A6L9S2.1|SYT_PARD8 (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0
Identity = 261/413 (63.20%), Postives = 318/413 (77.00%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            M +A SIS  LA+ VL+A VNGEVWD +RPIN+DA+V+LL W D EGK  FWHSSAHLMAEAL+ LYPG+KF IGP +ENGFYYDVD GE  I   DF  IEAKM+EL  +K   +R  ++KADA+  F ++ +EYK +L+  LEDG IT Y QG+FTDLCRGPH+PNT ++KA KI +VAGA+++GD+  KQL R+YGITFPK+  L EYLT++EEAKKRDHRK+G+E+DLF FSD VGKGLP+WLPKG  LR RL+ FL++ Q +  YQ V+ P IG+K LY TSGH+ KYG+DSFQPI TPEEGEE+ LKPMNCPHHC I+K  PRSYKDLP RLAEFGTV RYEQSGELHGLTRVR FTQDDAHIFC  DQV+ EF    D  + +F  +  ++    +  RD K+ R+
Sbjct:   21 MQIAESISSRLAQEVLAASVNGEVWDLSRPINDDATVKLLKWEDEEGKHAFWHSSAHLMAEALQELYPGIKFGIGPAIENGFYYDVDPGEAVIKEGDFAAIEAKMLELVAKKEEIKRQDITKADAMKMFGDRGEEYKTELISELEDGTITTYTQGSFTDLCRGPHLPNTSYLKAVKILSVAGAYWRGDEKRKQLVRLYGITFPKKKMLDEYLTLLEEAKKRDHRKIGKEMDLFMFSDTVGKGLPMWLPKGTALRLRLQEFLRRIQARYNYQEVMCPPIGNKLLYITSGHYAKYGKDSFQPIHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRLAEFGTVCRYEQSGELHGLTRVRSFTQDDAHIFCRPDQVKQEFLNVMDIISIVFKTMNFENFEAQISLRD-KVNRE 432          

HSP 2 Score: 65.4698 bits (158), Expect = 3.348e-10
Identity = 32/54 (59.26%), Postives = 41/54 (75.93%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +K+ DYA ++  +L  Q+IR  VD R+EKIGRKIRD E+ +IP MLIVGEKE E
Sbjct:  556 EKFNDYAWQIARELGNQEIRAIVDDRNEKIGRKIRDNELKRIPYMLIVGEKEAE 609          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|166223340|sp|A6GY22.1|SYT_FLAPJ (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 543.502 bits (1399), Expect = 0.000e+0
Identity = 254/409 (62.10%), Postives = 318/409 (77.75%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEK 409
            MDVA SISEGLARNV+SA  N    + +  +  D S+ L TW D +GK  FWHS++H+MA+ LE +YPG+K  +GP ++NGFYYDVD G+  I   DF KIE++++E++++K+ F+  P++KADA+  +K  D+EYK +L+  LEDG ITF     FTDLCRG HIPNTG IKA KI ++AGA+++GD+ NKQLTR+YG +FPKQ +L +YL ++EEAK+RDHRKLG+EL+LFAFS KVG+GLPLWLPKGA LR+RLE FLKKAQ K GY+ VVTPHIG K+LY TSGH+ KYG DSFQPI TP EGEEF+LKPMNCPHHCEI+  RP SYKDLP R AEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQ+++EFKK  D    +F  L  ++    +  RD++
Sbjct:   21 MDVAKSISEGLARNVISAAFNNITVEISTELTTDGSLVLYTWNDKDGKKAFWHSTSHVMAQVLEEMYPGLKLTLGPAIDNGFYYDVDFGDTKIVEADFKKIESRILEISKEKHEFKLRPVTKADALELYK--DNEYKFELISNLEDGTITFCDHATFTDLCRGGHIPNTGIIKAVKIMSIAGAYWRGDEKNKQLTRVYGTSFPKQKDLTDYLELLEEAKRRDHRKLGKELELFAFSQKVGQGLPLWLPKGAALRDRLEQFLKKAQKKGGYEQVVTPHIGQKELYVTSGHYAKYGADSFQPISTPTEGEEFLLKPMNCPHHCEIYNVRPWSYKDLPKRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTPDQLDEEFKKVIDLVLYVFGSLGFENFTAQISLRDKE 427          

HSP 2 Score: 64.3142 bits (155), Expect = 8.116e-10
Identity = 33/70 (47.14%), Postives = 46/70 (65.71%), Query Frame = 0

Query:  365 DDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            + A I C +D       KYE YA ++   L+  +IR  +D+R+E IGRKIRDAE+ K P MLIVGE+E++
Sbjct:  545 EQAIILCLSD-------KYEIYAKKVLNLLENHEIRALIDNRNESIGRKIRDAEMQKTPFMLIVGEEEEK 607          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|238691475|sp|B2RJD6.1|SYT_PORG3 (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 540.036 bits (1390), Expect = 0.000e+0
Identity = 267/413 (64.65%), Postives = 322/413 (77.97%), Query Frame = 0

Query:    2 DVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEH-SISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            D+A SIS  L ++VL+A VNG+VWD +RPINEDA V+L  W D EGK  FWHSSAHLMAEALE LYPG+KF IGP +ENGFYYDVD GE  SI   D   IE +M +LA +K T  R  ++KADA+  F +KDD+YK++L+  L DG IT Y QG FTDLCRGPH+PNTG+IKA K+ +VAGA+++GD+  KQLTRIYGI+FPK+  L EYL ++EEAKKRDHRK+G+EL+LFAFS  VG GLPLWLP+G  LR RLE+FLK+ Q   GYQ V+TPHIG+K LY TSGH+ KYG+DSF+PI TP+EGEEFMLKPMNCPHHCEIFK  P SY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAH+FC  DQ+++EF K  D    +F  L  ++    +  RD K+ R+
Sbjct:   22 DIAGSISPRLQQDVLAAGVNGQVWDLHRPINEDAEVKLFKWDDAEGKHAFWHSSAHLMAEALEELYPGIKFGIGPAIENGFYYDVDPGEGISIKDADLPAIEKRMQDLAARKETIIRRDIAKADALRMFGDKDDQYKVELISELADGTITTYTQGGFTDLCRGPHLPNTGYIKAIKLLSVAGAYWRGDEKRKQLTRIYGISFPKKKMLDEYLELLEEAKKRDHRKIGKELELFAFSQNVGAGLPLWLPRGTQLRLRLEDFLKQIQKHFGYQQVITPHIGNKNLYITSGHYAKYGQDSFRPINTPQEGEEFMLKPMNCPHHCEIFKITPHSYRDLPIRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFCRPDQLKEEFCKVMDIIFIIFKALDFKNFEAQISLRD-KVNRE 433          

HSP 2 Score: 60.4622 bits (145), Expect = 1.282e-8
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +++ +YA+ +  +L ++DIR  VD R+EK+GRKIRD E+ +IP MLIVGE E  E
Sbjct:  557 ERFNEYAHRVAKELNQRDIRVQVDDRNEKVGRKIRDNELKRIPYMLIVGENESRE 611          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|229470721|sp|B6YQ95.1|SYT_AZOPC (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 538.88 bits (1387), Expect = 0.000e+0
Identity = 255/408 (62.50%), Postives = 315/408 (77.21%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSN-DFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            M +A SIS  LA  +LSA VNGE+WD +RPINE+ASV+L  W D EGK TFWHSSAHL+AE+L+ LYP + F +GP +ENGFYYDVD G+  +  + D   IE KM ELA +K +  R  ++K +A+  F +K + YK++L+  LEDG IT Y QGNFTDLCRGPH+PNT +IKA K+T+VA A++KGD+S KQLTRIYGI+FPK+ EL EYL ++E+AKKRDHR++G+E++LF FS KVG+GLPLWLPKG  LR RLE+FLK+ Q K GYQ V+TPHIG+K LY  SGH+ KYG+DSFQPI TPE GEEF+LKPMNCPHHCEI+K   RSYK+LP RLAEFGTVYRYE+SGEL GLTRVRGFTQDDAHIFC+ +QV++E  K  D    +F  L   D    +  RD
Sbjct:   21 MQIAESISCRLAEEILSANVNGEMWDLSRPINENASVKLYKWEDFEGKRTFWHSSAHLLAESLQELYPNIHFGMGPAIENGFYYDVDLGDGVVIKDVDLVTIEKKMQELASKKLSISRQNVTKQNALELFGKKGETYKIELISKLEDGKITVYTQGNFTDLCRGPHLPNTSYIKAIKLTSVARAYWKGDESRKQLTRIYGISFPKKEELSEYLALMEKAKKRDHRRIGKEMELFTFSHKVGRGLPLWLPKGTQLRLRLEDFLKRIQKKYGYQQVITPHIGNKTLYIASGHYAKYGKDSFQPIHTPELGEEFLLKPMNCPHHCEIYKAFLRSYKELPLRLAEFGTVYRYERSGELQGLTRVRGFTQDDAHIFCSPEQVKEELIKVIDIVRVIFCALNFNDYEVQISLRD 428          

HSP 2 Score: 60.4622 bits (145), Expect = 1.358e-8
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 0

Query:  383 YEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQ 433
            Y  YA E+  +  ++DIR  +D R+EK+GRKIRD E+ KIP +LI+GEKE+
Sbjct:  559 YNQYAEEIAKEFHKRDIRVTIDKRNEKVGRKIRDNELKKIPYLLIIGEKER 609          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|73919814|sp|Q7MVQ5.1|SYT_PORGI (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 536.569 bits (1381), Expect = 0.000e+0
Identity = 266/413 (64.41%), Postives = 321/413 (77.72%), Query Frame = 0

Query:    2 DVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEH-SISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            D+A SIS  L ++VL+A VNG+VWD +R INEDA V+L  W D EGK  FWHSSAHLMAEALE LYPG+KF IGP +ENGFYYDVD GE  SI   D   IE +M +LA +K T  R  ++KADA+  F +KDD+YK++L+  L DG IT Y QG FTDLCRGPH+PNTG+IKA K+ +VAGA+++GD+  KQLTRIYGI+FPK+  L EYL ++EEAKKRDHRK+G+EL+LFAFS  VG GLPLWLP+G  LR RLE+FLK+ Q   GYQ V+TPHIG+K LY TSGH+ KYG+DSF+PI TP+EGEEFMLKPMNCPHHCEIFK  P SY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAH+FC  DQ+++EF K  D    +F  L  ++    +  RD K+ R+
Sbjct:   22 DIAGSISPRLQQDVLAAGVNGQVWDLHRQINEDAEVKLFKWDDAEGKHAFWHSSAHLMAEALEELYPGIKFGIGPAIENGFYYDVDPGEGISIKDADLPAIEKRMQDLAARKETIIRRDIAKADALRMFGDKDDQYKVELISELADGTITTYTQGGFTDLCRGPHLPNTGYIKAIKLLSVAGAYWRGDEKRKQLTRIYGISFPKKKMLDEYLELLEEAKKRDHRKIGKELELFAFSQNVGAGLPLWLPRGTQLRLRLEDFLKQIQKHFGYQQVITPHIGNKNLYITSGHYAKYGQDSFRPINTPQEGEEFMLKPMNCPHHCEIFKITPHSYRDLPIRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFCRPDQLKEEFCKVMDIIFIIFKALDFKNFEAQISLRD-KVNRE 433          

HSP 2 Score: 60.4622 bits (145), Expect = 1.260e-8
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +++ +YA+ +  +L ++DIR  VD R+EK+GRKIRD E+ +IP MLIVGE E  E
Sbjct:  557 ERFNEYAHRVAKELNQRDIRVQVDDRNEKVGRKIRDNELKRIPYMLIVGENESRE 611          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|189083608|sp|A5FP01.1|SYT_FLAJ1 (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0
Identity = 255/409 (62.35%), Postives = 316/409 (77.26%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEK 409
            M+VA +ISEG ARNV+SA  NG   ++  P+  D ++ L TW D EGK  FWHS++H+MA+ALE LYPG+K  +GP + NGFYYDVD  +  IS  DF KIE +++E+AR K  F+  P++KA+A+  +K  D+ YK +L+  LEDG ITF     FTDLCRG HIPNTG IKA KI +VAGA+++GD+ NKQLTR+YG +FPKQ +L EYL ++EEAK+RDHRKLG+EL+LFAFS KVG+GLPLWLPKGA LR+RLE FLK+AQ KAGY+ VV+PHIG K+LY TSGH+ KYG DSFQPI TP EGEEF+LKPMNCPHHCEI+  RP SYKDLP R AEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  +Q+++EFKK  D    +F  L  ++    +  RD++
Sbjct:   21 MEVAKNISEGFARNVISASFNGTTIETETPLTTDGNLILYTWNDAEGKKAFWHSTSHVMAQALEELYPGIKLTLGPAIANGFYYDVDFEDQKISEADFKKIEDRILEIARGKFDFKMRPVTKAEALEMYK--DNVYKTELISNLEDGTITFCDHATFTDLCRGGHIPNTGIIKAVKIMSVAGAYWRGDEKNKQLTRVYGTSFPKQKDLTEYLELLEEAKRRDHRKLGKELELFAFSQKVGQGLPLWLPKGAALRDRLEQFLKRAQKKAGYEQVVSPHIGQKELYVTSGHYAKYGADSFQPIHTPAEGEEFLLKPMNCPHHCEIYNVRPWSYKDLPKRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTPEQLDEEFKKVIDLVLYVFGSLGFENFTAQISLRDQE 427          

HSP 2 Score: 64.3142 bits (155), Expect = 8.406e-10
Identity = 29/54 (53.70%), Postives = 43/54 (79.63%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +KYE+YA ++   L+  +IR  +D+R+E IG+KIRDAE+ KIP MLIVGE+E++
Sbjct:  554 EKYENYAKKVLDLLENHEIRALIDNRNETIGKKIRDAEMQKIPFMLIVGEEEEK 607          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|73919769|sp|Q8AAP2.1|SYT_BACTN (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 525.013 bits (1351), Expect = 0.000e+0
Identity = 249/413 (60.29%), Postives = 309/413 (74.82%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            + +A SIS  LA++VL+  VNGE +D  RPINEDA   L  W D EGK  FWH+SAHL+AEAL+ LYPG++F IGP +ENGFYYDVD GE  I  +D   IEAKM+EL+ +K+   R  +SK DA+  F ++ + YK +L+  LEDG+IT Y QG FTDLCRGPH+  T  IKA K+T VAGA+++G +  K LTRIYGITFPK+  L EYL ++EEAKKRDHRK+G+E+ LF FS+ VGKGLP+WLPKG  LR RL+ FL++ Q +  YQ V+TP IG+K LY TSGH+ KYG+D+FQPI TPEEGEE+ LKPMNCPHHCEI+K  PRSYKDLP R+AEFGTV RYEQSGELHGLTRVR FTQDDAHIFC  DQV+DEF +  D  + +F  +  Q+    +  RD K+ R+
Sbjct:   21 LQIAESISSRLAQDVLACGVNGETYDLGRPINEDADFVLYKWDDEEGKHAFWHTSAHLLAEALQELYPGIQFGIGPAIENGFYYDVDPGEAVIKESDLPAIEAKMLELSAKKDAVVRESISKTDALKMFGDRGETYKCELISELEDGHITTYTQGAFTDLCRGPHLMTTAPIKAIKLTTVAGAYWRGHEDRKMLTRIYGITFPKKKMLDEYLVLLEEAKKRDHRKIGKEMQLFMFSETVGKGLPMWLPKGTALRLRLQEFLRRIQTRYDYQEVITPPIGNKLLYVTSGHYAKYGKDAFQPIHTPEEGEEYFLKPMNCPHHCEIYKNFPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHIFCRPDQVKDEFLRVMDIISIVFRSMNFQNFEAQISLRD-KVNRE 432          

HSP 2 Score: 62.3882 bits (150), Expect = 3.735e-9
Identity = 30/54 (55.56%), Postives = 41/54 (75.93%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +K+ +YA ++ + L+  +IR  VD R+EKIGRKIRD E+ +IP MLIVGEKE E
Sbjct:  556 EKFNEYAEQVKMYLKMHEIRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAE 609          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|73919767|sp|Q5LEQ6.1|SYT_BACFN (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS >gi|73919768|sp|Q64VP2.1|SYT_BACFR RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 523.087 bits (1346), Expect = 0.000e+0
Identity = 248/413 (60.05%), Postives = 309/413 (74.82%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            + +A SIS  LA++VL+  VNGE++D  RPINEDASV L  W D +GK  FWH+SAHL+AEAL+ LYPG++F IGP +ENGFYYDVD GE  I   D   IEAKM EL  +K    R  ++K DA+  F ++ + YK +L+  LEDG+IT Y QG+FTDLCRGPH+  T  IKA K+T+VAGA+++G +  K LTRIYGITFPK+  L EYL ++EEAKKRDHRK+G+E+ LF FSD VGKGLP+WLPKG  LR RL++FL++ Q +  YQ V+TP IG+K LY TSGH+ KYG+D+FQPI TPEEGEE+ LKPMNCPHHCEI+K  PRSYKDLP R+AEFGTV RYEQSGELHGLTRVR FTQDDAHIFC  DQV+ EF +  D  + +F  +   +    +  RD K+ R+
Sbjct:   21 LQIAESISSRLAQDVLACGVNGEIYDLGRPINEDASVVLYKWEDEQGKHAFWHTSAHLLAEALQELYPGIQFGIGPAIENGFYYDVDPGEAVIKEADLPAIEAKMAELVAKKEAVVRRDIAKGDALKMFGDRGETYKCELISELEDGHITTYTQGDFTDLCRGPHLMTTAPIKAIKLTSVAGAYWRGHEDRKMLTRIYGITFPKKKMLDEYLALMEEAKKRDHRKIGKEMQLFMFSDTVGKGLPMWLPKGTALRLRLQDFLRRIQTRYDYQEVITPPIGNKLLYVTSGHYAKYGKDAFQPIHTPEEGEEYFLKPMNCPHHCEIYKNFPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHIFCRPDQVKGEFLRVMDIISIVFRSMDFDNFEAQISLRD-KVNRE 432          

HSP 2 Score: 60.4622 bits (145), Expect = 1.209e-8
Identity = 30/54 (55.56%), Postives = 41/54 (75.93%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +K+ +YA ++   L+ ++IR  VD R+EKIGRKIRD E+ +IP MLIVGEKE E
Sbjct:  556 EKFNEYAEKVKTYLKMKEIRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAE 609          

BLAST of threonyl-trna synthetase vs. SwissProt
Match: gi|166223322|sp|A6L7J7.1|SYT_BACV8 (RecName: Full=Threonine--tRNA ligase; AltName: Full=Threonyl-tRNA synthetase; Short=ThrRS)

HSP 1 Score: 523.087 bits (1346), Expect = 0.000e+0
Identity = 247/413 (59.81%), Postives = 309/413 (74.82%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRDEKIGRK 413
            + +A SIS  LA++VL+  VNGE  D +RPINEDA+  L  W D +GK  FWH+SAHL+AEAL+ LYPG++F IGP +ENGFYYDVD GE  I   D   IEAKM ELA +K    R  ++K DA+  F E+ + YK +L+  LEDG+IT Y QG FTDLCRGPH+ +T  IKA K+  VAGA+++G +  KQ+TRIYGITFPK+  L EYL M+EEAKKRDHRK+G+E+DLF F+D VGKGLP+WLPKG  LR RL++FL++ Q +  YQ V+ P IG+K LY TSGH+ KYG+DSFQPI TPEEGEE+ LKPMNCPHHC I+K  PRSYKDLP R+AEFGTV RYEQSGELHGLTRVR FTQDDAH+FC  DQV++EF +  D  N +F  +  +++   +  RD K+ R+
Sbjct:   21 LQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLYKWEDEQGKHAFWHTSAHLLAEALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPAIEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCELISELEDGHITTYTQGAFTDLCRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKKRDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGNKNLYITSGHYAKYGKDSFQPIHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEFLRVMDIINIVFKSMNFENVEAQISLRD-KVNRE 432          

HSP 2 Score: 63.1586 bits (152), Expect = 1.786e-9
Identity = 30/54 (55.56%), Postives = 42/54 (77.78%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +K+ +YA ++  +L++ D+R  VD R+EKIGRKIRD E+ +IP MLIVGEKE E
Sbjct:  556 EKFNEYAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAE 609          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|941702820|ref|WP_055143407.1| (threonine--tRNA ligase [Jiulongibacter sediminis] >gi|936517420|gb|KPM49299.1| threonyl-tRNA synthetase [Jiulongibacter sediminis])

HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0
Identity = 343/406 (84.48%), Postives = 370/406 (91.13%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHR 406
            MDVALSISEGLARNVLSAKVNG V D+NRPI ED+ +QLLTW D +GKSTFWHSSAHLMAEALE LYPGVKFW+GPPVENGFYYDVDTG+H+IS +DFGKIEAKM+ELAR+KN + R PMSKA+A++YFKEK+DEYKLDLLEGLEDG+ITFY QGNFTDLCRGPHIPNTGFIKAAKITNVAGA+FKGDQS KQLTRIYG+TFPKQ ELKEYL MVEEAKKRDHRKLGQEL++FAFS+KVGKGLPLWLPKGAMLRERLENFLKKAQ+KAGY  VVTPHIGSKKLYETSGHW+KYGEDSFQPIKTPEE EEFMLKPMNCPHHCEIFKTRPRSYK+LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC NDQVEDEFKK  D    +F  L  +D    +  R
Sbjct:   21 MDVALSISEGLARNVLSAKVNGTVVDANRPIEEDSDLQLLTWSDADGKSTFWHSSAHLMAEALEALYPGVKFWVGPPVENGFYYDVDTGDHTISQDDFGKIEAKMLELAREKNDYFRQPMSKAEAVAYFKEKNDEYKLDLLEGLEDGSITFYSQGNFTDLCRGPHIPNTGFIKAAKITNVAGAYFKGDQSQKQLTRIYGVTFPKQKELKEYLEMVEEAKKRDHRKLGQELEIFAFSEKVGKGLPLWLPKGAMLRERLENFLKKAQLKAGYLPVVTPHIGSKKLYETSGHWDKYGEDSFQPIKTPEEDEEFMLKPMNCPHHCEIFKTRPRSYKELPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRNDQVEDEFKKVIDLVLYVFKSLGFEDYSAQISLR 426          

HSP 2 Score: 100.138 bits (248), Expect = 7.459e-19
Identity = 45/55 (81.82%), Postives = 55/55 (100.00%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYEDYANE++L+LQ++DIRGYVDHRDEKIGRKIRDAE+NK+PLMLI+GEKE+EE
Sbjct:  556 EKYEDYANEVYLELQDKDIRGYVDHRDEKIGRKIRDAEVNKLPLMLILGEKEKEE 610          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1245965013|ref|WP_096194283.1| (threonine--tRNA ligase [Cytophagales bacterium TFI 002] >gi|1243937047|emb|SOE22782.1| threonyl-tRNA synthetase [Cytophagales bacterium TFI 002])

HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0
Identity = 329/406 (81.03%), Postives = 364/406 (89.66%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHR 406
            MDVALSISEGLARNVL+AKVNGEVWDSNR + +++++QLLTW D +GKSTFWHSSAHLMAEALE LYPG+KFW+GPPVENGFYYDVD GE +I+  DF KIE KMIELARQKN FERIP+SKADA+SYF+ K+DE+KLDLL GL+DG IT YKQGNFTDLCRGPHIPNTGFIKAAKI NVAGA+FKGDQ+ +QLTRIYG+TFPKQ EL+E+L  +EEAK+RDHRKLGQEL++FAFS+KVGKGLPLWLPKGAMLRERLENFLK+AQ++AGY  VVTPHIGSK+LY TSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYK+LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKK  D    +F  L   D    V  R
Sbjct:   21 MDVALSISEGLARNVLAAKVNGEVWDSNRSLPQESNLQLLTWNDDDGKSTFWHSSAHLMAEALESLYPGIKFWVGPPVENGFYYDVDAGEKTITHEDFAKIEQKMIELARQKNVFERIPISKADAVSYFENKNDEFKLDLLSGLDDGTITLYKQGNFTDLCRGPHIPNTGFIKAAKIMNVAGAYFKGDQTQRQLTRIYGVTFPKQKELEEHLKRLEEAKQRDHRKLGQELEIFAFSEKVGKGLPLWLPKGAMLRERLENFLKRAQLRAGYLPVVTPHIGSKQLYVTSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKELPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKVIDLVLYVFKSLGFDDFSAQVSLR 426          

HSP 2 Score: 102.449 bits (254), Expect = 1.356e-19
Identity = 47/55 (85.45%), Postives = 53/55 (96.36%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYEDYANE+FL LQE+DIRGY+DHRDEKIGRKIRDAE+NK+PLMLIVGEKE EE
Sbjct:  556 EKYEDYANEVFLRLQEEDIRGYIDHRDEKIGRKIRDAEVNKVPLMLIVGEKEMEE 610          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|504843732|ref|WP_015030834.1| (threonine--tRNA ligase [Emticicia oligotrophica] >gi|387857049|gb|AFK05146.1| threonyl-tRNA synthetase [Emticicia oligotrophica DSM 17448])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 323/406 (79.56%), Postives = 362/406 (89.16%), Query Frame = 0

Query:    2 DVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            DVALSISEGLARNVL+AKVNG V D+N PI  D+++QLLTW DT+GKSTFWHSSAHLMAEALE LYP VKFW+GPP+ENGFYYDVDT   +IS++DF KIE KM+ELARQKN + RIPM+KA+AI YF+EK DEYKLDLL GLEDG+ITFYKQGNFTDLCRGPHIPNTGFIKAAKI NVAGA+FKGDQ+NK LTRIYG+TFPKQ EL +YL M+EEAK+RDHR+LGQEL+LF FS+KVGKGLPLWLPKGAMLRERLENFL+KAQVKAGYQ VVTPHIGSK+LY+TSGHWEKYG+DSFQPI TPEEGEEF+LKPMNCPHHCEIF++RPRSY++LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC +DQVE+EFKK  D    +F  L  +D    V  RD
Sbjct:   22 DVALSISEGLARNVLAAKVNGTVQDANLPIETDSTLQLLTWNDTDGKSTFWHSSAHLMAEALETLYPKVKFWVGPPLENGFYYDVDTNGEAISNDDFKKIEDKMLELARQKNDYIRIPMAKAEAIKYFEEKGDEYKLDLLSGLEDGSITFYKQGNFTDLCRGPHIPNTGFIKAAKIMNVAGAYFKGDQNNKMLTRIYGVTFPKQKELDDYLQMLEEAKRRDHRRLGQELELFTFSEKVGKGLPLWLPKGAMLRERLENFLRKAQVKAGYQQVVTPHIGSKQLYQTSGHWEKYGKDSFQPINTPEEGEEFLLKPMNCPHHCEIFRSRPRSYRELPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTDDQVENEFKKVIDLVLYVFKSLGFEDFSAQVSLRD 427          

HSP 2 Score: 103.99 bits (258), Expect = 3.398e-20
Identity = 49/55 (89.09%), Postives = 53/55 (96.36%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYEDYANE+FL LQEQDIRGYVDHRDEKIGRKIRDAE+NK+PLMLIVGEKE EE
Sbjct:  556 EKYEDYANEVFLTLQEQDIRGYVDHRDEKIGRKIRDAEVNKLPLMLIVGEKEAEE 610          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1325848341|ref|WP_101727041.1| (threonine--tRNA ligase [Emticicia sp. TH156] >gi|1319730746|gb|PLK43703.1| threonine--tRNA ligase [Emticicia sp. TH156])

HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 324/406 (79.80%), Postives = 362/406 (89.16%), Query Frame = 0

Query:    2 DVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            DVALSISEGLARNVL+A+VNG+V D+++PI ED+++QLLTW DT+GKSTFWHSSAHLMAEALE LYPGVKFW+GPP+ENGFYYDVDT   +IS++DF KIE KM+ELARQKN +ERIPMSKADA+ YF+EK DEYKLDLL GLEDG+ITFY+QGNFTDLCRGPHIPNTGFIKAAKI NVAGA+FKGDQ+NK LTRIYGITFPKQ EL EYL  +EEAK+RDHRKLGQEL+LF FS+KVGKGLPLWLPKGAMLRERLENFL++AQVKAGYQ VVTPHIG K+LY TSGHWEKYG+DSFQPI TPEEGEEFMLKPMNCPHHCEI+++RPRSY++LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQVEDEFKK  D    +F  L   +    V  RD
Sbjct:   23 DVALSISEGLARNVLAARVNGKVQDASQPIEEDSTLQLLTWNDTDGKSTFWHSSAHLMAEALEALYPGVKFWVGPPLENGFYYDVDTNGVTISNDDFRKIEDKMLELARQKNDYERIPMSKADAVKYFEEKGDEYKLDLLSGLEDGSITFYRQGNFTDLCRGPHIPNTGFIKAAKIMNVAGAYFKGDQNNKMLTRIYGITFPKQKELDEYLQFLEEAKRRDHRKLGQELELFTFSEKVGKGLPLWLPKGAMLRERLENFLRRAQVKAGYQQVVTPHIGGKQLYITSGHWEKYGKDSFQPIATPEEGEEFMLKPMNCPHHCEIYRSRPRSYRELPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCREDQVEDEFKKVIDLVLYVFKALGFDNFSAQVSLRD 428          

HSP 2 Score: 100.908 bits (250), Expect = 3.917e-19
Identity = 47/55 (85.45%), Postives = 52/55 (94.55%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYEDYAN++FL LQE DIRGYVDHRDEKIGRKIRDAE+NK+PLMLIVGEKE EE
Sbjct:  557 EKYEDYANDVFLTLQENDIRGYVDHRDEKIGRKIRDAEVNKLPLMLIVGEKESEE 611          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1099519059|emb|SFP99076.1| (threonyl-tRNA synthetase [Pseudarcicella hirudinis])

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 302/407 (74.20%), Postives = 350/407 (86.00%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            MD+ALSISEGLARNVL+AKVNG+V D++ PINED+ V LLTW DT+GK TFWHSSAHL+AEALE LYPG KF IGP +ENGFYYD+D GE S+S +DF KIE KM+ELARQKN F R  +SKADAI YF EK+DEYKL+L++GLEDG+ITFY QGNFTDLCRGPHIPNTGFIKA K+ +VAGA+++GD+  KQ+TR+Y ITFPKQ EL EYL ++EEAK+RDHRKLG+ELDLFAFS+KVG GLPLWLPKGAMLRERLENFL++AQV+AGY  VVTPHIGSK+LY TSGH+ KYG DSF+PI TP+E EEF+LKPMNCPHHCEIFKT+PRSY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC +DQVE+EF K  D    +F  L  ++    +  RD
Sbjct:   44 MDIALSISEGLARNVLAAKVNGKVQDASLPINEDSEVALLTWNDTDGKQTFWHSSAHLLAEALEALYPGTKFGIGPAIENGFYYDIDMGEKSLSQDDFKKIEDKMLELARQKNEFSRKAVSKADAIGYFTEKNDEYKLELIDGLEDGSITFYSQGNFTDLCRGPHIPNTGFIKAVKLMSVAGAYWRGDEKRKQMTRVYAITFPKQKELDEYLHLLEEAKRRDHRKLGKELDLFAFSEKVGMGLPLWLPKGAMLRERLENFLRRAQVRAGYSPVVTPHIGSKQLYVTSGHYAKYGADSFKPIHTPDENEEFLLKPMNCPHHCEIFKTKPRSYRDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTSDQVEEEFMKVIDLVLYVFKALGFENFSAQISLRD 450          

HSP 2 Score: 95.1301 bits (235), Expect = 3.075e-17
Identity = 44/55 (80.00%), Postives = 50/55 (90.91%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYE+YANELFL LQE D+RGYVDHRDEKIGRKIRDAE++K+P MLIVGEKE  E
Sbjct:  579 EKYENYANELFLRLQEVDLRGYVDHRDEKIGRKIRDAEVSKVPFMLIVGEKESAE 633          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1149994294|ref|WP_077920963.1| (threonine--tRNA ligase [Spirosoma sp. 209])

HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0
Identity = 307/407 (75.43%), Postives = 345/407 (84.77%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            +D+A  ISEGLARNVL+AKVNG+V D+ +PINEDASVQLLTW D EGK+TFWHSSAHL+AEALE LYPGVKF IGP VE GFYYDVD      S  DF K+E KM+ELARQK  + R PMSKADAI+YF+EK D YKLDLLEGL+DG ITFY QG FTDLCRGPHIPNTGFIKAAK+ NVAGA+++G++ NKQLTRIY +T+PKQ EL EYL ++EEAKKRDHRKLG+ELDLFAFS++VG GLPLWLPKG MLRERLENFL+KAQ++AGYQ VVTPHIGSK+LY TSGHW+KYGEDSFQPIKTP+  EEF+LKPMNCPHHCEI+KTRPRSY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQV+DEF K  D    +F  L   D    V  RD
Sbjct:   27 LDIATQISEGLARNVLAAKVNGKVQDATQPINEDASVQLLTWNDAEGKATFWHSSAHLLAEALESLYPGVKFGIGPAVETGFYYDVDLNGQPFSQEDFKKVEDKMLELARQKQDYIRKPMSKADAIAYFEEKGDPYKLDLLEGLDDGTITFYTQGQFTDLCRGPHIPNTGFIKAAKLMNVAGAYWRGNEKNKQLTRIYAVTYPKQKELDEYLHLLEEAKKRDHRKLGKELDLFAFSERVGAGLPLWLPKGTMLRERLENFLRKAQIRAGYQQVVTPHIGSKQLYVTSGHWDKYGEDSFQPIKTPDPNEEFLLKPMNCPHHCEIYKTRPRSYRDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRPDQVKDEFMKVIDLVLYVFKSLGFSDYSAQVSLRD 433          

HSP 2 Score: 90.1225 bits (222), Expect = 1.272e-15
Identity = 41/55 (74.55%), Postives = 48/55 (87.27%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYEDYAN+L+  LQE DIRG+VD RDEKIGRKIRDAE+NK+P ML+VGEKE  E
Sbjct:  562 EKYEDYANDLYFTLQEHDIRGFVDLRDEKIGRKIRDAEVNKVPFMLVVGEKEAAE 616          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1011389034|ref|WP_062301753.1| (threonine--tRNA ligase [Roseivirga seohaensis] >gi|1005899548|gb|KYG83063.1| threonine--tRNA ligase [Roseivirga seohaensis])

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 308/408 (75.49%), Postives = 352/408 (86.27%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHS-ISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            MDVALSISEGLARNVL+A++NGEV D+NRPI  D+ V+LLTW + EGK+TFWHSSAHLMAEALE LYPG+KF IGP +ENGFYYDVD GE+     +   KIEAKM+ELARQKN ++R  +SKADAI+YFKEKDDEYKLDLLEGLEDG+ITFY+QGNFTDLCRGPHIPNTGFIKAAK+ ++AGA+++GD++NKQLTR+YGITFPKQ EL EYL M+EEAKKRDHRKLGQEL+LF FS KVG GLPLWLPKGA LRERLE F++KAQVKAGY  VVTPHIGSK LY TSGH+EKYG DSFQPIKTP EGEEF+LKPMNCPHHCEI+K++PRSYK+LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQV++EF K  D    +F  L  ++    +  RD
Sbjct:   25 MDVALSISEGLARNVLAAEINGEVRDANRPIESDSDVKLLTWNNDEGKATFWHSSAHLMAEALEALYPGIKFGIGPSIENGFYYDVDFGENQEFDHSHLEKIEAKMLELARQKNEYKRSEVSKADAIAYFKEKDDEYKLDLLEGLEDGSITFYQQGNFTDLCRGPHIPNTGFIKAAKVLSIAGAYWRGDENNKQLTRLYGITFPKQKELTEYLHMLEEAKKRDHRKLGQELELFTFSQKVGLGLPLWLPKGAKLRERLETFMRKAQVKAGYDPVVTPHIGSKNLYVTSGHYEKYGADSFQPIKTPNEGEEFLLKPMNCPHHCEIYKSKPRSYKELPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTEDQVKEEFIKVIDLVLYVFESLGFENFTAQISLRD 432          

HSP 2 Score: 85.8853 bits (211), Expect = 3.292e-14
Identity = 40/54 (74.07%), Postives = 47/54 (87.04%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +KY DYA EL+  LQ +DI G+VDHRDEKIGRKIRDAE+ KIPLMLIVGEKE++
Sbjct:  561 EKYADYAQELYNKLQSEDITGFVDHRDEKIGRKIRDAEVKKIPLMLIVGEKERD 614          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1223987205|ref|WP_092018032.1| (threonine--tRNA ligase [Pseudarcicella hirudinis])

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 302/407 (74.20%), Postives = 350/407 (86.00%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            MD+ALSISEGLARNVL+AKVNG+V D++ PINED+ V LLTW DT+GK TFWHSSAHL+AEALE LYPG KF IGP +ENGFYYD+D GE S+S +DF KIE KM+ELARQKN F R  +SKADAI YF EK+DEYKL+L++GLEDG+ITFY QGNFTDLCRGPHIPNTGFIKA K+ +VAGA+++GD+  KQ+TR+Y ITFPKQ EL EYL ++EEAK+RDHRKLG+ELDLFAFS+KVG GLPLWLPKGAMLRERLENFL++AQV+AGY  VVTPHIGSK+LY TSGH+ KYG DSF+PI TP+E EEF+LKPMNCPHHCEIFKT+PRSY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC +DQVE+EF K  D    +F  L  ++    +  RD
Sbjct:   21 MDIALSISEGLARNVLAAKVNGKVQDASLPINEDSEVALLTWNDTDGKQTFWHSSAHLLAEALEALYPGTKFGIGPAIENGFYYDIDMGEKSLSQDDFKKIEDKMLELARQKNEFSRKAVSKADAIGYFTEKNDEYKLELIDGLEDGSITFYSQGNFTDLCRGPHIPNTGFIKAVKLMSVAGAYWRGDEKRKQMTRVYAITFPKQKELDEYLHLLEEAKRRDHRKLGKELDLFAFSEKVGMGLPLWLPKGAMLRERLENFLRRAQVRAGYSPVVTPHIGSKQLYVTSGHYAKYGADSFKPIHTPDENEEFLLKPMNCPHHCEIFKTKPRSYRDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTSDQVEEEFMKVIDLVLYVFKALGFENFSAQISLRD 427          

HSP 2 Score: 95.1301 bits (235), Expect = 3.413e-17
Identity = 44/55 (80.00%), Postives = 50/55 (90.91%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQEE 435
            +KYE+YANELFL LQE D+RGYVDHRDEKIGRKIRDAE++K+P MLIVGEKE  E
Sbjct:  556 EKYENYANELFLRLQEVDLRGYVDHRDEKIGRKIRDAEVSKVPFMLIVGEKESAE 610          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|1113409115|gb|OJW71712.1| (threonine--tRNA ligase [Spirosoma sp. 48-14])

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 307/407 (75.43%), Postives = 346/407 (85.01%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHSISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            +++A  ISEGLARNVL+AKVNG V D+ RPI EDA+VQLLTW DTEGK+TFWHSSAHL+AEALE LYPGVKF IGP +E GFYYDVD G    S  DF K+E KM+ELARQK  + R PMSKADAI+YF+EK D YKLDLLEGL+DG ITFY QGNFTDLCRGPHIPNTGFIKAAK+ NVAGA+++G++ NKQLTRIY +T+PKQ EL EYL ++EEAKKRDHRKLG+EL+LFAFS+KVG GLPLWLPKG MLRERLENFL+KAQ++AGY  VVTPHIGSK+LY TSGHWEKYGEDSFQPIKTP+  EEF+LKPMNCPHHCEI+KTRPRSY+DLP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQV+DEF K  D    +F  L  +D    V  RD
Sbjct:   27 LEIAAQISEGLARNVLAAKVNGVVQDATRPIEEDANVQLLTWNDTEGKATFWHSSAHLLAEALEALYPGVKFGIGPAIETGFYYDVDLGGQPFSQEDFKKVEDKMLELARQKEQYIRKPMSKADAIAYFEEKGDPYKLDLLEGLDDGTITFYSQGNFTDLCRGPHIPNTGFIKAAKLMNVAGAYWRGNEKNKQLTRIYAVTYPKQKELDEYLFLLEEAKKRDHRKLGKELELFAFSEKVGAGLPLWLPKGTMLRERLENFLRKAQLRAGYSPVVTPHIGSKQLYVTSGHWEKYGEDSFQPIKTPDPNEEFLLKPMNCPHHCEIYKTRPRSYRDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRPDQVKDEFMKVIDLVLYVFKSLGFEDYSAQVSLRD 433          

HSP 2 Score: 87.8113 bits (216), Expect = 7.707e-15
Identity = 41/52 (78.85%), Postives = 46/52 (88.46%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKE 432
            +KYEDYAN+LF  LQE DIRG+VD RDEKIGRKIRDAE+ K+P MLIVGEKE
Sbjct:  562 EKYEDYANDLFFSLQEHDIRGFVDLRDEKIGRKIRDAEVAKVPYMLIVGEKE 613          

BLAST of threonyl-trna synthetase vs. nr
Match: gi|921287849|ref|WP_053224450.1| (threonine--tRNA ligase [Roseivirga seohaensis] >gi|917715559|gb|KOF01932.1| threonyl-tRNA synthetase [Roseivirga seohaensis subsp. aquiponti])

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 308/408 (75.49%), Postives = 352/408 (86.27%), Query Frame = 0

Query:    1 MDVALSISEGLARNVLSAKVNGEVWDSNRPINEDASVQLLTWRDTEGKSTFWHSSAHLMAEALEVLYPGVKFWIGPPVENGFYYDVDTGEHS-ISSNDFGKIEAKMIELARQKNTFERIPMSKADAISYFKEKDDEYKLDLLEGLEDGNITFYKQGNFTDLCRGPHIPNTGFIKAAKITNVAGAFFKGDQSNKQLTRIYGITFPKQGELKEYLTMVEEAKKRDHRKLGQELDLFAFSDKVGKGLPLWLPKGAMLRERLENFLKKAQVKAGYQAVVTPHIGSKKLYETSGHWEKYGEDSFQPIKTPEEGEEFMLKPMNCPHHCEIFKTRPRSYKDLPFRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCANDQVEDEFKKYEDYANELFLDLQEQDIRGYVDHRD 407
            MDVALSISEGLARNVL+A++NGEV D+NRPI  D+ V+LLTW + EGK+TFWHSSAHLMAEALE LYPG+KF IGP +ENGFYYDVD GE+     +   KIEAKM+ELARQKN ++R  +SKADAI+YFKEKDDEYKLDLLEGLEDG+ITFY+QGNFTDLCRGPHIPNTGFIKAAK+ ++AGA+++GD++NKQLTR+YGITFPKQ EL EYL M+EEAKKRDHRKLGQEL+LF FS KVG GLPLWLPKGA LRERLE F++KAQVKAGY  VVTPHIGSK LY TSGH+EKYG DSFQPIKTP EGEEF+LKPMNCPHHCEI+K++PRSYK+LP RLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC  DQV++EF K  D    +F  L  ++    +  RD
Sbjct:   25 MDVALSISEGLARNVLAAEINGEVRDANRPIESDSDVKLLTWNNDEGKATFWHSSAHLMAEALEALYPGIKFGIGPSIENGFYYDVDFGENQEFDHSHLEKIEAKMLELARQKNEYKRSEVSKADAIAYFKEKDDEYKLDLLEGLEDGSITFYQQGNFTDLCRGPHIPNTGFIKAAKVLSIAGAYWRGDENNKQLTRLYGITFPKQKELTEYLHMLEEAKKRDHRKLGQELELFTFSQKVGLGLPLWLPKGAKLRERLETFMRKAQVKAGYDPVVTPHIGSKNLYVTSGHYEKYGADSFQPIKTPNEGEEFLLKPMNCPHHCEIYKSKPRSYKELPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTEDQVKEEFIKVIDLVLYVFESLGFENFTAQISLRD 432          

HSP 2 Score: 85.8853 bits (211), Expect = 3.413e-14
Identity = 40/54 (74.07%), Postives = 47/54 (87.04%), Query Frame = 0

Query:  381 KKYEDYANELFLDLQEQDIRGYVDHRDEKIGRKIRDAEINKIPLMLIVGEKEQE 434
            +KY DYA EL+  LQ +DI G+VDHRDEKIGRKIRDAE+ KIPLMLIVGEKE++
Sbjct:  561 EKYADYAQELYNKLQSEDITGFVDHRDEKIGRKIRDAEVKKIPLMLIVGEKERD 614