glutamate synthase, maker-scaffold3_size1495701-snap-gene-8.0 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of glutamate synthase vs. L. salmonis genes
Match: EMLSAG00000004194 (supercontig:LSalAtl2s:LSalAtl2s221:469662:476711:-1 gene:EMLSAG00000004194 transcript:EMLSAT00000004194 description:"augustus_masked-LSalAtl2s221-processed-gene-4.2") HSP 1 Score: 435.647 bits (1119), Expect = 1.010e-127 Identity = 274/733 (37.38%), Postives = 386/733 (52.66%), Query Frame = 0
Query: 13 IVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDP---IKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDD----FYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSG-RSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDII-------------------------------VLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLK-------------SSGPRRKEV-----DFDNIV---------------RNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEGIEAA--LNRINNEVEMIAHDSNI--ICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQ 660
I + A + MEHRGA A+ ++GDGAG+L+ IP +++ SE+N+ R Y G+ F + +KK+F K ++ + REVP D+ +GS+A SEP + Q FV ++ +EN+ F IL T D FYI S S IVYKGQ + Q+ YF DL D F S + IVH+RFSTNT P W+ A P R ++HNGEINT++GN+N EARE + + D L + PI +P +SDSG DNV EFL+ G RSLP A+M ++PEAWQNDD +PS KK+FY + MEPWDGPA + FTDG VGA LDRNGLRPSRY IT+++ + SE G + IDP++I+KK RL+PGK+L++D D +++I D E+KS+I P+ D++ K DLK SS PRR+ + DF+N R FG++ E ++++ M KE +GSMG D PLA LS++ YFKQ FAQVTNPPID REK MSL+ +G G I++ G + L L +PIL+ + L+ + + + + ++ I + ++ I N E + + LSD V + + V LA G+VHH +I+ VE G+ E HH L+ +GADAI PY+ +
Sbjct: 42 IFEDAAELSRRMEHRGACSADNDSGDGAGVLIAIPKDYYQKKL--SEVNVQLPRLYATGIIFCDESDESDVKKDFEK---HAESLKGNLRVIHWREVPKDSNSLGSVARASEPKLLQVFVTSEEEG----IENKCFDKYIYILRKTATHYIPACKDSRKRFYICSLSRTAIVYKGQFNPCQIWNYFTDLMDPEFESHLLIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNVNLMEAREGVMVSPDFED--LSTLYPIVEPDMSDSGCLDNVVEFLVHVGDRSLPEAMMTLVPEAWQNDDQMPSNKKNFYHWASFYMEPWDGPALLTFTDGRYVGAILDRNGLRPSRYYITKNNRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEVGVIDIDPTQIIKKGRLQPGKILLVDTDVQKVIDDHELKSSIAXSRPHGDFLNKSI----DLKIIKEAGSEYAAEISSLPRRRPINLHENDFNNNFDYGEENILEKLWRGDRRLPIFGYNIETLEMLMLPMFKTKKEALGSMGNDTPLACLSEMQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLECPIGPEGNILNPG--DCTRLWLKNPILSVNDLEHLKRTSILGWKSRVIDITYLSDDQISLSDIVSNICNRAEEAVKKEGVQLLILSDRNVGVNRIPVSAALASGAVHHHLIATRLRSKCAIIVESGEAREVHHLCVLIGFGADAICPYMVLE 757 HSP 2 Score: 373.244 bits (957), Expect = 1.155e-106 Identity = 205/421 (48.69%), Postives = 268/421 (63.66%), Query Frame = 0
Query: 812 MNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMY-ASQKQNHELEHVLDRELI--MQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRS-LNIHFRGSAGQSFGAFAAHGLSLVLE 1228
MN++GGKSN+GEGGE+E R+ + D SAIKQVASGRFGV+ +YL +A E+QIKMAQGAKPGEGG+LPG+KV IA+ R S P ARISVKLVS++GVGVIA+GVAK A+HILISG DGGTGAS + I+ GLPWELG++E HQTLV N LR RVVLQ DGQ+RTG D+ +A +LGA+E+G +TA L+V GC MMRKCHLNTCPVGIATQD LRKKF G+ EH+VNYF +A +R MA +G + NDL+G T+ L++ KT LN +LL+ E + S Q+ +L LD E+I T + + + ++ + I +T R G LS IA Y G+ + + +NI GS G++F A + G++ V++
Sbjct: 845 MNKLGGKSNTGEGGENEERYLN----DIRRSAIKQVASGRFGVSASYLAHADELQIKMAQGAKPGEGGELPGYKVTVEIAKTRRSVP--------------------------------ARISVKLVSEVGVGVIAAGVAKGKAEHILISGHDGGTGASSWTGIKGAGLPWELGIAETHQTLVMNNLRSRVVLQTDGQLRTGFDVMVAGILGADEFGFSTAPLIVLGCTMMRKCHLNTCPVGIATQDPILRKKFEGQPEHVVNYFFLIAEEIREFMAKLGCTKFNDLIGHTEFLKVTENPIPSKTHLLNFNSLLHSAESMRIPKKHGGSIPQDFQLNLRLDNEVIKACDTIINSDSLNPISLEYPIVNTQRTFGATLSYHIAMKYNESGLPRDNIINISLHGSGGRNFAAGMSGGIAYVVK 1229
BLAST of glutamate synthase vs. SwissProt
Match: gi|1707984|sp|P55037.1|GLTB_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 1; AltName: Full=Fd-GOGAT) HSP 1 Score: 1322.38 bits (3421), Expect = 0.000e+0 Identity = 691/1479 (46.72%), Postives = 948/1479 (64.10%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDH-DQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRF---KEEEGIEAALNRINNEV-EMIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMI---SR------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQ-VIARKWH-NEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF----GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDK--------------------------------------------VSRNA------TMNRIGGKSNSGEGGEDEFRFK-SLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLD-RELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVF-LRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKA 1406
+ +K + IV+ L +L N+EHRGA G E NTGDGAGIL+Q+PH F + A I IP Y VG + DP+ + + + + + R++P N +G A+ SEP M+Q ++ + + D E +L+ +R L + + +Y++S S++T+VYKG L+ Q+ Y+ +L D SA+A+VHSRFSTNT P W+ + P+R I+HNGEINT++GN+NW +AR+ S + V P+ + SDS FDN E L +GRSLPHA+MMMIPE W +S+ KK+FY+YH +MEPWDGPASI FT+G ++GA LDRNGLRPSRY +T+DD++++ SEAG L I+P R+ KK RL+PG+M ++D+++ RIIADEE+K I ++PY +W+ ++ L S G D +++ + Q+AFG++ E+ ++L M G E IGSMGAD PLA+LS + NYF+Q FAQVTNPPIDS+RE+ S +T +GG G ++D + ++ L +PILT E +L+ LDD F + + F + E G++ AL+ + E + I+ +N+I LSD +V A+P LLA+ +HH +I SR +E G+ E HHFA LL YG AI PYLA+++ +IA N D + +Y KAA KG++K+ SK+G+STI SYRGAQ FEA+G++QSV+ + F T SR+ G D +E +H+ AF GDL + L G +QW++ GE HLF+PQ+IHLLQ AV+ ++ YK +A +++ +S+ A MNRIGGKSN+GEGGED RF + + GDS SAIKQVASGRFGVT YL+ AKEIQIKMAQGAKPGEGGQLPG KV IA+VR+STPGVGLISPPPHHDIYSIEDLA+LI DLKNAN+EARI+VKLVS++GVG IA+GVAKAHAD +L+SG DGGTGASP +SI+H GLPWELGL+E HQTLV N LR R+V++ DGQM+TGRD+AIA +LGAEE+G +TA LV GCIMMR CHLNTCPVGIATQ+ ELR KF G H VN+ F+A LR +MA +G ++N++VGRTD+LE HWK + ++L +L++ E G D Y Q+H L+H LD +L+ A + +V I + NR VGT++ NEI K + +G+ + ++++HF+GSAGQSFGAF G++L LEG+ANDY+ KGLSG +I + P + S F + +NII GNV LYGAT+GE+ I GM G+RF VRNSG + VVE +G +GCEYMTGG V+VLG G NF AGMSGG A+I+ + N MV ++ LE +E+ L++ I+ T+S +L DWE F+K+ P++ K +L+A
Sbjct: 47 FIVQMKGKVSHDIVEQGLQMLVNLEHRGACGCEPNTGDGAGILIQVPHKFIQKIAGAEGITIPAPGQYAVGNIYGSPDPLARAEARQKFNDIVAQEGLKVLGWRDIPTQNEPLGETAIASEPFMQQVYIARPEGLTDDLDFERKLYVIRK--LTHGAIRSPKIDTYWYVASLSARTLVYKGMLTTAQVGQYYPELHDPDMESALALVHSRFSTNTFPSWERSHPYRYIAHNGEINTMRGNVNWMQARQALFE-SSLFGEDMAKVQPVINIDGSDSTIFDNALELLYLAGRSLPHAVMMMIPEPWSAHESMSQEKKAFYKYHSCLMEPWDGPASIAFTNGKMMGAVLDRNGLRPSRYYVTKDDLVIMASEAGVLPIEPERVAKKGRLQPGRMFLVDMEQGRIIADEEIKQEIVSQHPYGEWLAANLKSLEQLPSPG-NVPGTDAESLRQRQMAFGYTFEELRILLAPMGRDGVEAIGSMGADTPLAVLSDKPKLLYNYFQQLFAQVTNPPIDSIREEIITSAETTIGGEGNLLDPRPESCRLIELKTPILTNEDLAKLKALDDDEFKSVTLDILFDPNQGEAGLKTALDNLFTEADQAISQGANLIILSDRQVSAEKAAIPALLAVSGLHHHLIRNGSRTKVGLVLESGEPREVHHFAVLLGYGCGAINPYLAFETLDGMIAEGLLVNVDHKTACKNYIKAATKGVIKVASKIGISTIQSYRGAQIFEAVGLNQSVIDEYFCRTSSRIQGSDLGVIAQEAILRHQHAFAPRPGDL--HTLDVGGEYQWRKDGEEHLFSPQTIHLLQRAVREGNYELYKQYAALVNEQNQKFFTLRGLLDFQDRESIPLEEVEPIEAIMKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEGGEDPERFTWTNDQGDSKNSAIKQVASGRFGVTSLYLSQAKEIQIKMAQGAKPGEGGQLPGKKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAELIHDLKNANREARINVKLVSEVGVGTIAAGVAKAHADVVLVSGYDGGTGASPQTSIKHAGLPWELGLAETHQTLVLNNLRSRIVVETDGQMKTGRDVAIAALLGAEEFGFSTAPLVSLGCIMMRACHLNTCPVGIATQNPELRAKFTGDPAHAVNFMTFIATELREVMAQLGFRTINEMVGRTDILEPKKAVAHWKAKGIDLSTILHQPEVGDDVGRYCQIPQDHGLQHSLDITQLLDLCQPAIAKGEKVTATLPITNINRVVGTIVGNEITKRH-WEGLPEDTVHLHFQGSAGQSFGAFIPKGMTLELEGDANDYLGKGLSGGKIIVYPPKGSSFIASENIIAGNVCLYGATAGEVYISGMVGERFCVRNSGVNTVVEAVGDHGCEYMTGGKVVVLGQTGRNFAAGMSGGVAYIFDETGDFATRCNSAMVGLEKLEDPEEIKDLKELIQNHVNYTDSAKGKAVLADWEASIPKFVKVMPRDYKRVLQA 1518
BLAST of glutamate synthase vs. SwissProt
Match: gi|239938894|sp|P39812.3|GLTA_BACSU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=NADPH-GOGAT) HSP 1 Score: 1294.64 bits (3349), Expect = 0.000e+0 Identity = 687/1471 (46.70%), Postives = 929/1471 (63.15%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFV-KYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEGIEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSC-QVIARKWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDK-------------------------------------------VSRNA------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIAN-TDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTL-ARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEIL-RNINHEMVSVDTLEVADEV-FLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKA 1406
L LK T IV L +L ++HRG G++ +TGDGAG+LVQIP FF+ IN+P YGVGM FF + ++ + I I V + R VPV+ IG++A +S P + Q F+ D N E +L+ +R N + DFY +S SS+TIVYKG L+ Q+ ++ DLQD+ F SA A+VHSRFSTNT P W+ A P R + HNGEINT++GNINW ARE+Q +SE+ L +LPI + SDS DN EF + +GR H MM+IPE W + + K++FYEYH ++MEPWDGP +I FTDG +GA LDRNGLRP+RY +T+DD I+ SE G + ++ ++ K+RLEPGKML+IDL+E RII+DEEVK+ I YPYQ W+E++ V V+ S R+E F +++ Q AFG++ ED L + +GK+P+GSMG D PLA+LS AQ NYFKQ FAQVTNPPID++RE+ S T+LG G ++ E ++L +P+L+ E F L+ + + ++ + F E+ +E L + + E I+ +++ LSD +++E +P LLA+ ++H +I + VE G+ E HHFA L+ YGADAI PYLAY + Q I + E ++ Y K+ +G++K+MSK+G+ST+ SYRGAQ FEA+G+S+ V+ + F GT S++GG+D +E +HR A+ D L QW+ GE H FNP++IH LQ A +RND++ +K + K D+ +S+ A MNR+GGKSNSGEGGED RF ENGD SAIKQ+ASGRFGV +YL NA E+QIKMAQGAKPGEGGQLPG+KV +A VR STPGVGLISPPPHHDIYSIEDLAQLI DLKNAN++ARISVKLVSK GVG IA+GVAKA AD I+ISG DGGTGASP +SI+HTGLPWELGL+EAHQTL+ N LRDRVVL+ DG++ TGRD+ +A +LGAEE+G ATA LVV GC+MMR CHL+TCPVG+ATQ+ ELRKKF G +H+VNY F+A +R MA +G + ++++GRTD+L + HWK L+L LLY +P +T + QNH+++ LD I+ A + E + +I +TNR GT+ +EI+K YG +G+ + ++ +HF GSAGQSFGAF G++L L+G++NDYV KGLSG +I + AS N+I+GNVA YGATSGE I G AG+RF VRNSG + VVEGIG +GCEYMTGGSV+VLG +G NF AGMSGG A++ + + R N EM+ ++LE E+ ++ +++ TNS A ++L WE F+K+ PK K +L +
Sbjct: 26 LYAHLKGKQTHDIVKQGLKMLCQLDHRGGQGSDPDTGDGAGLLVQIPDAFFRKEC--KNINLPEKERYGVGMVFFSQKEDERKKIEKQINALIEQEGQVVLGWRTVPVNVGKIGTVAQKSCPFVRQVFIGASSDLKDNLSFERKLYVIRKQAENWGVTEGL----DFYFASLSSQTIVYKGLLTPEQVDAFYSDLQDEAFVSAFALVHSRFSTNTFPTWERAHPNRYLVHNGEINTLRGNINWMRAREQQF-VSESFGEDLNKILPILNADGSDSSILDNAFEFFVMAGRKPAHTAMMLIPEPWTENTHMSKEKRAFYEYHSSLMEPWDGPTAISFTDGKQIGAILDRNGLRPARYYVTKDDYIIFSSEVGVIEVEQENVLYKNRLEPGKMLLIDLEEGRIISDEEVKTQIATEYPYQKWLEEELVQVNPDPES---REEEQFSDLLTRQKAFGYTYEDIQKYLIPVIKEGKDPLGSMGNDAPLAVLSDRAQSLFNYFKQLFAQVTNPPIDAIREQLVTSTMTWLGAEGDLLHPSERNVRRIKLYTPVLSNEQFYALKTIVHPDLKSQKIDVLFSED--LERGLKDMFTQAEKAISQGVSLLILSDKKMNERLTPIPPLLAVSALHQHLIRKGLRTKVSIIVESGEAREVHHFAALIGYGADAINPYLAYATYKQEIDEGRLDISYEEAVSKYGKSITEGVVKVMSKMGISTVQSYRGAQIFEAVGISRDVIDRYFSGTASQLGGIDLQTIAEEAQRRHREAYQDDYSKTLEPGSDFQWRNGGEHHAFNPKTIHTLQWACRRNDYNLFKQYTKAADEERIGFLRNLFAFDGNRKPLKLEEVESAESIVKRFKTGAMSFGSLSKEAHEALAIAMNRLGGKSNSGEGGEDPKRFVPDENGDDRRSAIKQIASGRFGVKSHYLVNADELQIKMAQGAKPGEGGQLPGNKVYPWVADVRGSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANRDARISVKLVSKAGVGTIAAGVAKATADVIVISGYDGGTGASPKTSIKHTGLPWELGLAEAHQTLMLNGLRDRVVLETDGKLMTGRDVVMAALLGAEEFGFATAPLVVLGCVMMRACHLDTCPVGVATQNPELRKKFMGDPDHIVNYMLFIAEEVREYMAALGFKTFDEMIGRTDVLHASERAKEHWKASQLDLSTLLY--QPEGVRTFQSP--QNHKIDQSLDITTILPAVQEAIESGKEADISIEINNTNRVAGTITGSEISKRYGEEGLPEDTIKLHFTGSAGQSFGAFVPKGMTLYLDGDSNDYVGKGLSGGKIIVKSSEGFNSASDDNVIIGNVAFYGATSGEAYINGRAGERFAVRNSGVNVVVEGIGDHGCEYMTGGSVVVLGDVGKNFAAGMSGGIAYVLTEDVKAFKRKCNLEMILFESLEDEKEIQQIKAMLERHTAYTNSQKAEDLLDQWEDSVKKFVKVIPKNYKQMLAS 1480
BLAST of glutamate synthase vs. SwissProt
Match: gi|417073|sp|Q03460.1|GLSN_MEDSA (RecName: Full=Glutamate synthase [NADH], amyloplastic; AltName: Full=NADH-GOGAT; Flags: Precursor) HSP 1 Score: 1267.29 bits (3278), Expect = 0.000e+0 Identity = 695/1527 (45.51%), Postives = 933/1527 (61.10%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLR-------SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQL-RGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEARE-----KQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSS--------------GP-RRKEVDFDNIVRNQL-----AFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEE---EGIEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSC---QVIAR-------KWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF-------GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENG--DSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIAN--TDNHWKTRDLNLEALLY---KFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVF-LRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKA 1406
V +L +R V AL +L M HRGA G E NTGDGAGILV +PH F++ + +P Y VGMFF PK ++ K+I K + R VP DNT +G A +EP +EQ F+ D DLE Q++ LR +S LN Q D I DFYI S SS+T++YKGQL+ QL Y+ DL ++RFTS +A++HSRFSTNT P W AQPFR + HNGEINT++GN+NW +ARE K+L +SE + L+ LPI D SDSG FD V EFLL SG+SLP A+MMMIPEAWQND ++ +K+FYEY+ A+MEPWDGPA I FTDG +GA LDRNGLRP R+ +T +++ SE G + I P + +K RL PG ML++D +++ ++ D+ +K PY DW+EKQ + + D+ S P +VD +N+ L AFG+S E +++L MA G E +GSMG D PLA++S + YFKQ FAQVTNPPID +REK S++ +G G + + E++ L L P+L+ + + ++ ++ + + + + +E +G+E AL+RI E I+ + LSD + ++AV +LLA+G+VH ++ VE + E HHF TL+ +GADAI PYLA ++ QV + ++++DE L+ Y KA+ G++K+++K+G+ST+ SY+GAQ FEALG+S V+ KCF GT SRV G F ++ H LAF G LP+ G + W++ GE HL +P +I LQ A + N YK ++K I ++++ MN IGGKSN+GEGGE R + L +G + SAIKQVASGRFGV+ YLTNA E+QIKMAQGAKPGEGG+LPGHKV +IA RNST GVGLISPPPHHDIYSIEDLAQLI DLKNAN ARISVKLVS+ GVGVIASGV K HA+H+LISG DGGTGAS + I+ GLPWELGL+E HQTLV N LR R LQ DGQ++TGRD+AIA +LGAEE+G +TA L+ GCIMMRKCH NTCPVGIATQD LR+KF G+ EH++N+F +A +R IM+ +G +VN++VGR+D+LE+ + K +++L LL + P + Q Y QKQ+H L+ LD +LI + A V I +TNRAVGTMLS+E+ K Y G+ +++I F GSAGQSFGAF G++L LEG++NDY+ KGLSG ++ + P + S F NI++GNVALYGAT GE GMA +RF VRNSGA AVVEG+G +GCEYMTGG+V+VLG G NF AGMSGG A++ N E+V +D +E +++ LR I++ TNS +A +L D+E L F+K+FP+E K +L +
Sbjct: 106 FVAELNGQSSRKTVTDALEMLVRMTHRGACGCEANTGDGAGILVALPHGFYQEVV---DFQLPPQGNYAVGMFFLPKSDSRRKESKNIFTKVAESLGHKVLGWRSVPTDNTGLGKSAQLTEPVIEQVFLT-PSSDSKVDLEKQMYILRKLSMVSITSALN--LQSDG--ITDFYICSLSSRTVIYKGQLTPAQLGEYYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREGLLKCKELGLSE---NDLKKFLPIVDANSSDSGCFDGVLEFLLHSGKSLPEAVMMMIPEAWQNDKNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIPPEDVCRKGRLNPGMMLLVDFEKQIVVNDDALKEQYSLARPYGDWLEKQKIELKDIIDSVHESDIVPPTISGVPPLSNDDVDMENMGIQGLLAPLKAFGYSVESLEILLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMRCMVGPEGDLTETTEEQCHRLSLKGPLLSTKEMEAIKKMNYRGWRSKVIDITYSKERGTKGLEEALDRICTEAHNAISEGYTTLVLSDRAFSKKHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDE--LVKKYFKASTYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELAFPSRIFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTIHELNKACNLRGLLKFKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMNTIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGLAETHQTLVANDLRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGFRTVNEMVGRSDMLEVDKEVVKGNAKLENIDLSLLLRPAAELRPEAAQ--YCVQKQDHGLDMALDNKLISLSNAALEKGLPVYIETPICNTNRAVGTMLSHEVTKRYNLAGLPADTIHIQFTGSAGQSFGAFLCPGITLELEGDSNDYIGKGLSGGKVVVYPPKGSNFDPKDNILIGNVALYGATRGEAYFNGMAAERFCVRNSGALAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGTFQSRCNLELVDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFENLLPKFVKVFPREYKRVLAS 1617
BLAST of glutamate synthase vs. SwissProt
Match: gi|81556736|sp|P96218.3|GLTB_MYCTU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain) HSP 1 Score: 1267.29 bits (3278), Expect = 0.000e+0 Identity = 665/1496 (44.45%), Postives = 928/1496 (62.03%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQ----QDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSK--LRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDN--IVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSR--------VSLRFKEEEG---IEAALNRINNEVEMIAHDSNIIC-LSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNE--DEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDL----RQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDK---------------------------------VSRNAT-------------------MNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPG-SDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILP--DRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKA 1406
+V D+ +R IVD A+T L N+EHRGA GAE +GDGAGIL+Q+P F + + +P Y G+ F P+ + ++K + + R VP D++ +G+++ ++ P Q F+ LE + + +R ++ QD + Y S S +T+VYKG L+ QL+ ++LDLQD+R TSA+ IVHSRFSTNT P W LA PFR I+HNGEINT+ GN NW ARE + ++ S + + PIC P SD+ FD V E L GRSL HA++MMIPEAW+ +S+ +++FY+YH ++MEPWDGPAS+ FTDGT+VGA LDRNGLRPSR +T+D ++V+ SEAG L + PS +V++ RL+PG+M ++D + RI++DEE+K+ + +PYQ+W++ V +D+L P K+V + IV QLAFG++ E+ +L++ MA G EPIGSMG D P+A+LS+ + +YF Q FAQVTNPP+D++RE+ SL+ GG +++ ++ + L PIL +L LD ++ R + ++ EG + AAL + D I LSD E DE +P+LLA+ VHH ++ VE GD E HH A L+ +GA AI PYL ++S + + + E D + +Y KAA KG+LK+MSK+G+ST+ SY GAQ F+A+G+S+ V+ + F G GG+ ++ A+HRLA+ D L G +QW+R+GE+HLFNP+++ LQH+ + + +K + + +D V R +T MNR+G +SN GEGGED RF NGD SAIKQVAS RFGVT +YLTN ++QIKMAQGAKPGEGGQLPGHKV +A VR+STPGVGLISPPPHHDIYSIEDLAQLI DLKNAN AR+ VKLVS+ GVG +A+GV+KAHAD +LISG DGGTGA+PL+S++H G PWELGL+E QTL+ N LRDR+V+Q DGQ++TGRD+ IAT+LGAEE+G ATA LVV GCIMMR CHL+TCPVG+ATQ+ LR++F GK E + N+F F+A +R +A +G +VN+ VG+ L+ HWK L+L +L++ E +Q +Y S +Q+H L+ LD++LI+ + A + V I + NR VGTML +E+ K YG +G+ +++I F GSAG SFGAF G++L + G+ANDYV KGLSG I + P D + + NII GNV L+GATSGE+ +RG+ G+RF VRNSGAHAVVEG+G +GCEYMTGG V++LG G NF AGMSGG A++Y E+ N+N EMV ++TL+ D +L I+ + T+S V IL+DW + +F+K+ P++ K +L+A
Sbjct: 22 MVVDMHGRRSRDIVDKAITALLNLEHRGAQGAEPRSGDGAGILIQVPDEFLREAV---DFELPAPGSYATGIAFLPQSSKDAAAACAAVQKIAEAEGLQVLGWRSVPTDDSSLGALSRDAMPTFRQVFLAGA---SGMALERRCYVVRKRAEHELGTKGPGQDGPGRETVYFPSLSGQTLVYKGMLTTPQLKAFYLDLQDERLTSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALIK-TDIFGSAADVEKLFPICTPGASDTARFDEVLELLHLGGRSLAHAVLMMIPEAWERHESMDPARRAFYQYHASLMEPWDGPASMTFTDGTVVGAVLDRNGLRPSRIWVTDDGLVVMASEAGVLDLHPSTVVRRMRLQPGRMFLVDTAQGRIVSDEEIKADLAAEHPYQEWLDNGLVPLDEL----PEGKDVRMPHHRIVMRQLAFGYTYEELNLLVAPMARLGAEPIGSMGTDTPVAVLSQRPRMLYDYFHQLFAQVTNPPLDAIREEVVTSLQGTTGGERDLLNPDQNSCHQIVLPQPILRNHELAKLVSLDPNDKVNGRPHGLRSKVIRCLYRVSEGGAGLAAALEEVRGAAAAAIADGARIIILSDRESDEEMAPIPSLLAVAGVHHHLVRERTRTQVGLVVESGDAREVHHMAALVGFGAAAINPYLVFESIEDMLDRGVIEGIDRTAALNNYIKAAGKGVLKVMSKMGISTLASYTGAQLFQAVGISEQVLDEYFTGLTCPTGGITLDDIAADVAARHRLAYLDRPDERAHRELEVGGEYQWRREGEYHLFNPETVFKLQHSTRTGQYKIFKEYTRLVDDQSERMASLRGLLKFRTGVRPPVPLDEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGARSNCGEGGEDVKRFDRDPNGDWRRSAIKQVASARFGVTSHYLTNCTDLQIKMAQGAKPGEGGQLPGHKVYPWVAEVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPSARVHVKLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTSMKHAGAPWELGLAETQQTLLLNGLRDRIVVQVDGQLKTGRDVMIATLLGAEEFGFATAPLVVAGCIMMRVCHLDTCPVGVATQNPLLRERFTGKPEFVENFFMFIAEEVREYLAQLGFRTVNEAVGQAGALDTTLARAHWKAHKLDLAPVLHEPESAFMNQDLYCSSRQDHGLDKALDQQLIVMSREALDSGKPVRFSTTIGNVNRTVGTMLGHELTKAYGGQGLPDGTIDITFDGSAGNSFGAFVPKGITLRVYGDANDYVGKGLSGGRIVVRPSDDAPQDYVAEDNIIGGNVILFGATSGEVYLRGVVGERFAVRNSGAHAVVEGVGDHGCEYMTGGRVVILGRTGRNFAAGMSGGVAYVYDPDGELPANLNSEMVELETLDEDDADWLHGTIQVHVDATDSAVGQRILSDWSGQQRHFVKVMPRDYKRVLQA 1506
BLAST of glutamate synthase vs. SwissProt
Match: gi|300680979|sp|Q0DG35.2|GLT2_ORYSJ (RecName: Full=Glutamate synthase 2 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 2; Short=NADH-GOGAT 2; Flags: Precursor) HSP 1 Score: 1266.52 bits (3276), Expect = 0.000e+0 Identity = 685/1514 (45.24%), Postives = 930/1514 (61.43%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRS-SILN--DTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFL-DLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLN---ISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSG--------------PRRKEVDFD----NIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEG---IEAALNRINNEVEMIAHDS-NIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSC---QVIARKWHNE----DEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF-------GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNAT--------------------------------------------------MNRIGGKSNSGEGGEDEFRFKSLENG--DSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANT--DNHWKTRDLNLEALL---YKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSV-DTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAIL 1404
+ +L +R VD A+ +L+ M HRGA G E+NTGDGAGILV +PH FF+ + +P Y VGMFF P D ++ K + R+ L + R VP DN+ +G A+++EP +EQ FV K D E Q++ LR S+++ + DFY+ S SS+TIVYKGQL QL+GYF DL D+ FTS +A++HSRFSTNT P W AQP R + HNGEINT++GN NW +ARE L + T D L+ +LPI D SDSG DNV E L++SGRS P A+MMMIPEAWQND ++ +K+ YE+ A+MEPWDGPA I FTDG +GA LDRNGLRP R+ +T +++ SE G + + P + +K RL PG ML++D + ++ D+E+K K PY +W+++Q + + D+ S P KE D I+ AFG++RE ++++ MA G+E +GSMG D PLA++S + YFKQ FAQVTNPPID +REK S++ +G G + + E + L L SP+L + ++ ++ + + + + ++ G ++ L++I + H+ I+ LSD +AV +LLA+G+VH ++S VE + E HHF+TL+ +GADAI PYLA ++ Q+ R N+ +E L+ Y A+ G++K+++K+G+ST+ SY+GAQ FEALG++ VVSKCF+GT SRV G F ++ H +AF G N LP+ G + W++ GE HL +P SI LQ A + N YK +++ I ++++ T MN +GGKSN+GEGGE R L +G + +SAIKQVASGRFGV+I YLTNA E+QIKMAQGAKPGEGG+LPGHKV +IA RNST GVGLISPPPHHDIYSIEDLAQLI DLKNAN ARISVKLVS+ GVG++ASGV K HADH+LISG DGGTGAS + I++ GLPWELGL+E HQTLV N LR R VLQ DGQM+TGRD+A+A +LGAEE+G +TA L+ GCIMMRKCH NTCP GIATQD LR KF GK EH++NYF LA +R IMA +G +VN++VGR+D+LEI + + K +++L LL + PG+ Q Y +KQ+H L+ LD +LI +T A V + + NRAVGTMLS+E+ K Y G+ +++I GSAGQSFGAF G++L LEG++NDYV KGLSG +I + P RNS+F NI++GNVALYGAT GE GMA +RF VRNSGA AVVEGIG +GCEYMTGG+ ++LG G NF AGMSGG A++Y + N+E+V + +E D LR I++ T S++A +IL +++ L FIK++P++ K +L
Sbjct: 111 FIAELSGEYSRKTVDDAIEMLDRMAHRGACGCEKNTGDGAGILVALPHNFFREVTKDAGFELPPPGEYAVGMFFMPTDDKRREKSKLLFREKAELLGHTVLGWRRVPTDNSGLGQSAVDTEPVIEQVFVT-KSASSKADFERQMYVLRRFSVMSIREVLGVKNGGTKDFYMCSLSSRTIVYKGQLKPSQLKGYFFADLGDESFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNKNWMKAREGLLKCEGLGLTRDEMLK-LLPIVDATSSDSGAIDNVLELLIQSGRSAPEAVMMMIPEAWQNDVNMDPERKALYEFFSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTYSGRVIMASEVGVVDVPPQDVSRKGRLNPGMMLLVDFENHCVVNDDELKKEYSKVRPYGEWLKRQRIQLTDIIESVNEAERIAPSISGALPITKENKADMGICGILTPLKAFGYTREALEMLMLPMAKDGQEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLSETTERQCHRLTLKSPLLNTNEMEAIKKMNYRGWRSKVLDITYPKKNGRMGLKQTLDKICAQAREAIHEGYTILVLSDRGFSSERVAVSSLLAVGAVHQHLVSHLERTRIGLLVESAEPREVHHFSTLIGFGADAICPYLAIEAIWRLQIDGRIPPNDGKPYTQEQLIEKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLASEVVSKCFEGTPSRVEGATFEMLAQDALRLHEIAFPSRTLPPGSADANALPNPGDYHWRKNGEVHLNDPFSIAKLQEAARINSREAYKEYSRRIYELNKACTLRGMLKFREIPNQISLDEVEPAKEIVKRFCTGAMSYGSISLEAHTSLAEAMNTLGGKSNTGEGGEQPCRMVPLPDGSKNPRISAIKQVASGRFGVSIYYLTNAVEVQIKMAQGAKPGEGGELPGHKVIGDIAVTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGIVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAVLQTDGQMKTGRDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPAGIATQDPVLRAKFAGKPEHVINYFFMLAEEVREIMAQLGFRTVNEMVGRSDMLEIDPKVLEGNEKLENIDLSRLLKPAAEISPGAVQ--YCVEKQDHGLDMALDNKLIASSTAALRKGVRVFIETPVRNINRAVGTMLSHEVTKRYHIHGLPSDTIHIKLNGSAGQSFGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRNSRFNPQDNIVIGNVALYGATKGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTAVILGKTGRNFAAGMSGGIAYVYDVDGKFSSRCNYELVDLYAVVEEDDITTLRMMIQQHRLHTQSDLARDILLNFDTLLPKFIKVYPRDYKRVL 1620
BLAST of glutamate synthase vs. SwissProt
Match: gi|300680981|sp|Q9LV03.2|GLUT1_ARATH (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor) HSP 1 Score: 1253.81 bits (3243), Expect = 0.000e+0 Identity = 684/1525 (44.85%), Postives = 928/1525 (60.85%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRS----SILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFL-DLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVL--PICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEV------------DFDN--------IVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEE---EGIEAALNRINNEV-EMIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSC---QVIARKWHNEDEEF-----LMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF-------GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENG--DSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIAN--TDNHWKTRDLNLEALLY---KFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLE-VADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSVV 1412
V +L TR V +L +L M HRGA G E NTGDGAGILV +PH F+ A +P + Y VGMFF P ++ K++ K + R VP DN+ +G+ AL++EP + Q F+ + D E Q++ LR +I Q A+ DFYI S SS+TIVYKGQL QL+ Y+ DL +RFTS +A+VHSRFSTNT P W AQP R + HNGEINT++GN+NW ARE L +E SK PI D SDSG FD V E L+R+GRSLP A+MMMIPEAWQND ++ +K FYEY A+MEPWDGPA I FTDG +GA LDRNGLRP R+ IT +++ SE G + + P +++K RL PG ML++D ++ ++ D+ +K PY +W+++Q + + D+ S P + + D D+ ++ AFG++ E +++L MA G E +GSMG D PLA++S + YFKQ FAQVTNPPID +REK S++ +G G + + E++ L L P+L E + ++ ++ + + + + +E +G+E L+RI +E E I ++ LSD +AV +L+A+G+VHH ++ VE + E HHF TL+ +GADAI PYLA ++ QV + + EF L+ Y KA+ G++K+++K+G+ST+ SY+GAQ FEALG+S V+ KCF GT SRV G F ++ H LAF G + L + G + W++ GE HL +P +I LQ A + N + YK ++K I+++++ + MN++GGKSN+GEGGE R + L +G + S+IKQ+ASGRFGV+ YLTNA E+QIKMAQGAKPGEGG+LPGHKV +IA RNST GVGLISPPPHHDIYSIEDLAQLI DLKNAN ARISVKLVS+ GVGVIASGV K HADH+LI+G DGGTGAS + I++ GLPWELGL+E HQTLV N LR R VLQ DGQ++TGRD+A+A +LGAEE+G +TA L+ GCIMMRKCH NTCPVGIATQD LR+KF G+ EH++N+F LA +R IM+ +G +V +++GR D+LE+ N+ K +++L LL + PG+ Q Y QKQ+H L+ LD+ELI + A + V I + NRAVGTMLS+E+ K Y G+ K +++I F GSAGQS GAF G+ L LEG++NDYV KGLSG ++ + P + S F +NI++GNVALYGATSGE GMA +RF VRNSGA AVVEG+G +GCEYMTGG+V+VLG G NF AGMSGG A++ + N E+V +D +E D++ L+ I++ TNS +A +L D+E L FIK+FP++ K +L A H V
Sbjct: 121 FVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSK-ADFEQQMYILRRVSMVAIRAALNLQHGAM-KDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDREVVKNNDKLENIDLSLLLRPAAEIRPGAAQ--YCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMKHEEV 1641
BLAST of glutamate synthase vs. SwissProt
Match: gi|46395959|sp|Q9C102.1|GLT1_SCHPO (RecName: Full=Putative glutamate synthase [NADPH]; AltName: Full=NADPH-GOGAT) HSP 1 Score: 1245.34 bits (3221), Expect = 0.000e+0 Identity = 674/1496 (45.05%), Postives = 926/1496 (61.90%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYD---MVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKD-HDQNGDLENQLFFLRSSILNDTYQQDAALIDD---FYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGR-SLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIV--------RNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDD--SNFSFSRVSLRFKEEEGIE---AALNRINNEVEMIAHDS-NIICLSDIEVDEHYLAVPTLLAIGSVHHFMI-----SRV----EGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNED---EEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFG---DLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNAT---------------------------------------------------MNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELI--MQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEV-FLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILK 1405
C +K + IV A +L NM HRGA GA+ GDGAG++ +P+ F + + +P S Y +G FF + + C+ + + + + + + R VP DN+++G AL EP + Q V K +D + + LF + +L +Q + LI FYI S +TIVYKGQL+ VQ+ YFLDL + + S A+VHSRFSTNT P W AQP R +HNGEINT++GN NW ARE + S + ++LPI + SDS FDNV E L SG SLP A+M++IPEAWQND ++ K +FYE+ MEPWDGPA F DG GA LDRNGLRP R+ +T DD+++ SE G + I+P RIV+K RL PG+ML++D E RI+ D+E+K I RY ++ W++++ + ++ + S VD IV + LAFG++ E ++I+ MA GKE +GSMG D +A LS + +YF+Q FAQVTNPPID +RE MSL+ Y+G SG ++++ + + LR+ +PILT E F+ L+ +D ++ + + + F + EG+ AA+ RI +E + ++ I LSD V+ + + ++ A G+VHH+++ SRV E GD E HH TLL YGADA+ PYLA ++ + R+ + EE + ++ A G+LK+MSK+G+ST+ SY+GAQ FEALG+ V++KCF GT SR+ G+ F + A H + +R +PD+G ++ GE H+ +P++I LQ AV+ + + Y F++ + +R T MNR+GGKSN+GEGGED R + L NGD+M SAIKQ+ASGRFGVT YL++A E+QIKMAQGAKPGEGG+LPG+KV ++IA+ R+ST GVGLISPPPHHDIYSIEDL QLI+D+K+AN AR+SVKLVS++GVG++ASGVAKA ADHIL+SG DGGTGAS + I++ GLPWELG++E HQTLV N LR RVV+Q DGQ+RTGRD+AIA +LGAEEWG AT L+ GCIMMRKCHLNTCPVGIATQD ELRKKF G+ EH+VN+F+++A LRGIMA +G ++N++VGR+D L++A N+ L F Y +KQ+H L LD +LI + TL S V +I +T+R +G LSN+I+K YG +G+ S+ ++ GSAGQSFGAF A G++L LEG+ NDYV KGLSG + I P R S F +N+I+GNV LYGATSG I G+A +RF VRNSGA AVVEG+G +GCEYMTGG V++LGS G NF AGMSGG A++Y Q + IN EMV + ++ A E+ FLR I+ T S VA IL+D+ F+K+ P+E KA+L+
Sbjct: 73 FTCHIKGQVSHKIVTDARLLLCNMTHRGATGADTRDGDGAGVMTGMPYTFMQKEFGQIGCTLPKSGEYAIGNVFFSPE---ADVCREAMTAFTQVAEKLGLAILAWRSVPCDNSILGPAALSREPTILQPCVVLKAAYDGEAEFDTDLFERQLYVLR---KQSSHLIGKEKWFYICSLHRETIVYKGQLAPVQVYNYFLDLNNAEYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMK-SSRFGEEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKAAFYEWAACQMEPWDGPALFTFADGRYCGANLDRNGLRPCRFYLTSDDMMICASEVGTVGIEPDRIVQKGRLYPGRMLLVDTKEGRIVDDKELKHNIASRYDFRSWLDQELIDMNSIVDSLIESTSVDLTPIVDDVPLADDKTMLAFGYTLEQINMIMAPMANGGKETLGSMGNDAAIACLSDQPRLLYDYFRQLFAQVTNPPIDPIREAIVMSLQCYIGPSGNLLEINQSQCRRLRMPTPILTVEEFNALKNVDRIYPDWKVASIDITFFKSEGVAGYAAAIERICSEADTAVNEGYKAIVLSDRNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDAREVHHMCTLLGYGADAVCPYLAMEALTKLVRQNAMKPGITEETAIKNFKHAINGGILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHERGYPTDQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSRTHYEQTRRCTLRGMLDFDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGGKSNTGEGGEDPARSQRLANGDTMRSAIKQIASGRFGVTSWYLSDADELQIKMAQGAKPGEGGELPGNKVSESIAKTRHSTAGVGLISPPPHHDIYSIEDLKQLIYDMKSANPRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAETHQTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALGCIMMRKCHLNTCPVGIATQDPELRKKFEGQPEHVVNFFYYVAEELRGIMAKLGFRTINEMVGRSDKLKVAEPINNKSKLLDLTPLLTPAFTLRPGAATYNVRKQDHRLYTRLDNKLIDEAEVTLEEGIPSVVE--CEIINTDRTLGATLSNKISKRYGEEGLPTDSIRVNVFGSAGQSFGAFLAPGVTLQLEGDCNDYVGKGLSGGRLIIYPPRVSPFKPEENMIIGNVCLYGATSGHAFISGVAAERFAVRNSGAIAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYVYDMQMDFAGKINTEMVDISSVTDAAEIAFLRGLIQDHRHYTGSQVADRILSDFPRHLSRFVKVLPREYKAVLE 1559
BLAST of glutamate synthase vs. SwissProt
Match: gi|122222486|sp|Q0JKD0.1|GLT1_ORYSJ (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor) HSP 1 Score: 1229.93 bits (3181), Expect = 0.000e+0 Identity = 677/1521 (44.51%), Postives = 927/1521 (60.95%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRS-SILNDTYQQDAAL------IDDFYISSFSSKTIVYKGQLSAVQLRGYFL-DLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISET--LDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGP------------------RRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSL------RFKEEEGIEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVI---ARKWHNED-----EEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF-------GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENG--DSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIAN--TDNHWKTRDLNLEALL---YKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEV-FLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAIL 1404
V +L R V+ AL +LE M HRGA G E+NTGDGAGILV +PH FF+ + +P Y VGM F P D ++ K+ +K V + R VP DN+ +G AL++EP +EQ F+ K D E QL+ LR SIL+ AAL DFY+ S SS+TIVYKGQL QL+GY+ DL + FTS +A+VHSRFSTNT P W AQP R + HNGEINT++GN NW +ARE L + + +LPI D SDSG FD V E L+R GRSLP A+MMMIPEAWQND ++ KK+ YE+ A+MEPWDGPA I FTDG +GA LDRNGLRP R+ +T +V+GSE G + + +++K RL PG ML++D + ++ DE +K+ K +PY +W+++Q +Y+ D+ S P +++ + IV AFG++ E +++L MA G E +GSMG D PLA++S + YFKQ FAQVTNPPID +REK S++ +G G +++ E + L L+ P+++ D +E + N+ R + + +G+E L+RI E I ++ LSD +AV +LLA+G+VH +++ VE + E HHF TL+ +GADA+ PYLA ++ + + N D +E L+ Y A+ G++K+++K+G+ST+ SY+GAQ FEALG+S V+ KCF GT SR+ G F ++ H LAF G LP+ G + W++ GE HL +P ++ LQ A + N + YK +++ I ++++ MN++GGKSN+GEGGE R + L NG + SAIKQVASGRFGV+ YLTNA E+QIKMAQGAKPGEGG+LPGHKV +IA R+ST GVGLISPPPHHDIYSIEDLAQLI DLKN+N ARISVKLVS+ GVGV+ASGV K HADH+LISG DGGTGAS + I++ GLPWELGL+E HQTLV N LR R +LQ DGQ++TG+D+A+A +LGAEE+G +TA L+ GCIMMRKCH NTCPVGIATQD LR+KF G+ EH++N+F LA LR IM+ +G ++ ++VGR+D+LE+ ++ K +++L +L + PG+ Q Y +KQ+H L+ LD +LI + A V I++TNRAVGTMLS+E+ K Y KG+ ++++ GSAGQS GAF G++L LEG++NDYV KGLSG +I + P R+S F NI++GNVALYGAT GE GMA +RF VRNSGA AVVEGIG +GCEYMTGG+V++LG G NF AGMSGG A++Y + NHE+V + +E +++ L+ I++ T S VA +IL++++ L F+K+FP++ K +L
Sbjct: 104 FVAELSGDYKRATVNDALEMLERMAHRGACGCEKNTGDGAGILVALPHNFFREVTKDAGFELPQPGEYAVGMVFLPIDEKRRERSKAEFQKVAESLGHVILGWRRVPTDNSDLGESALQTEPVIEQVFLT-KSSSSEADFEQQLYILRRLSILSIR----AALNLRRGGKRDFYMCSLSSRTIVYKGQLKPCQLKGYYYADLGHENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKLGLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGGRSLPEAVMMMIPEAWQNDVNMEPEKKALYEFLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVVMGSEVGVVDVPSKDVLRKGRLNPGMMLLVDFENHTVVDDEALKAQYSKAHPYGEWLKRQKIYLKDIVESVPETERVAPGISGSLTQKNEKKEHAGVNGIVTPLKAFGYTVEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLLETTEKQCNRLALEGPLVS---IDEMEAIKKMNYRGWRSKVLDITYPKKSGRKGLEETLDRICTEARGAIKKGYTVLVLSDRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVHHFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFPSRAPPPGSADAKALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQELNKTCNLRGMLKFKDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKLGGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAVTRHSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPRARISVKLVSEAGVGVVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAILQTDGQLKTGKDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMSQLGFRTITEMVGRSDMLEVDPEVVKSNEKLENIDLSLILKPAAEIRPGAAQ--YCVEKQDHGLDMALDNKLIALSKAALEKEVRVFIETPIQNTNRAVGTMLSHEVTKRYHMKGLPAGTIHVKLTGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRDSTFIPEDNIVIGNVALYGATIGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTVVILGKTGRNFAAGMSGGIAYVYDIDGKFSVRCNHELVDLYHVEEEEDITTLKMMIEQHRLNTGSVVARDILSNFDTLLPKFVKVFPRDYKRVL 1614
BLAST of glutamate synthase vs. SwissProt
Match: gi|729601|sp|Q05755.1|GLTB_AZOBR (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain; AltName: Full=NADPH-GOGAT; Flags: Precursor) HSP 1 Score: 1144.8 bits (2960), Expect = 0.000e+0 Identity = 617/1480 (41.69%), Postives = 888/1480 (60.00%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPI-KKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKE---VDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRL-EYLDDSNFSFSRVSLRFKEEEGIEA---ALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMIS---------RVEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARK--WHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKV-------------------------------------------------SRNATMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLL-EIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVAD-EVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDH 1409
+ + P R +V+ + L+ + HRGA A+ TGDGAGI V +P FFK P ++ VG F P+ + + C+ I+ I + R+VP++ +IG A + P++EQ V + E L+ +R I + I+DFYI S S+++I+YKG A QL ++ DL D+RF S AI H R+STNT P W LAQPFR ++HNGEINT++GN+NW +A E ++ + ++++ P+ +SDSG+ D V E ++R+GR+ P MM++P+A + + P K+ +Y +++MEPWDGPA++ TDG V +DRNGLRP RY IT D +I+ GSE G + ID +++++K RL PG+M+ +DL ++ D E+K + P+ W++ T ++D+L + + E +D + R Q AFG + ED +LIL M GKE IGSMG D P+A+LS + ++F+Q F+QVTNPPIDSLRE+ MSLKT LG G I+D E + +L+L+SP+LT F + +Y+ D+ + + F + G EA AL RI E E + + + L+D + A+P +LA G+VH +I V + +TH+FA L+ GA + YLA ++ R+ + + E MA+Y KA + GLLKIMSK+G+S I+SYRG FEA+G+S+++V++ F +SR+ G+ G QK++ +H A+ + LP G +++++ G+ H + IH LQ AV + ++T+K ++++++K + N MNRIG KS+SGEGGED RF+ +NGD+ SAIKQVASGRFGVT YL +E++IK+AQGAKPGEGGQLPG KV + IAR+R+STPGV LISPPPHHDIYSIEDLAQLI+DLK N +A+++VKLVS+ G+G IA+GVAKA+AD ILISG GGTGASP +SI+ GLPWE+GLSE HQ L N+LR RV L+ DG ++TGRDI IA MLGAEE+GI TA+L+ GCIM+R+CH NTCPVG+ QD +LR+KF G E +VN F FLA +R I+A +G S+N+++GRTDLL +++ H DL+L L + +PG + Y + + +E+ LD ++ ++ + +T RA+GT LS+ + + +G G+Q + I RG+AGQS GAFA G+ L + G+ANDYV KGLSG I + P +S ++KN I+GN LYGAT+G+L G AG+RF VRNSGA VVEG G NGCEYMTGG+ ++LG +G+NF AGM+GG A++Y D + IN E V +EV E L+ I++ T S A IL DW F ++ PKE+ L+ H
Sbjct: 41 FIAAIDGKPRRSVVEKGIEALKAVWHRGAVDADGKTGDGAGIHVAVPQKFFKDHVKVIGHRAPDNKL-AVGQVFLPRISLDAQEACRCIVETEILAFGYYIYGWRQVPINVDIIGEKANATRPEIEQIIVGNNKGVSDEQFELDLYIIRRRIEKAVKGEQ---INDFYICSLSARSIIYKGMFLAEQLTTFYPDLLDERFESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNWMKAHETRME-HPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRAGRTAPMVKMMLVPQALTSSQTTPDNHKALIQYCNSVMEPWDGPAALAMTDGRWVVGGMDRNGLRPMRYTITTDGLIIGGSETGMVKIDETQVIEKGRLGPGEMIAVDLQSGKLYRDRELKDHLATLKPWDKWVQ-NTTHLDELVKTASLKGEPSDMDKAELRRRQQAFGLTMEDMELILHPMVEDGKEAIGSMGDDSPIAVLSDKYRGLHHFFRQNFSQVTNPPIDSLRERRVMSLKTRLGNLGNILDEDETQTRLLQLESPVLTTAEFRAMRDYMGDTA---AEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHVILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAEGLDTHYFAVLIGVGATTVNAYLAQEAIAERHRRGLFGSMPLEKGMANYKKAIDDGLLKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGIGLNGIQKKVLEQHATAYNE-EVVALPVGGFYRFRKSGDRHGWEGGVIHTLQQAVTNDSYTTFKKYSEQVNKRPPMQLRDLLELRSTKAPVPVDEVESITAIRKRFITPGMSMGALSPEAHGTLNVAMNRIGAKSDSGEGGEDPARFRPDKNGDNWNSAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPGFKVTEMIARLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRSGIGTIAAGVAKANADIILISGNSGGTGASPQTSIKFAGLPWEMGLSEVHQVLTLNRLRHRVRLRTDGGLKTGRDIVIAAMLGAEEFGIGTASLIAMGCIMVRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREILAGLGFRSLNEVIGRTDLLHQVSRGAEHLD--DLDLNPRLAQVDPG-ENARYCTLQGRNEVPDTLDARIVADARPLFEEGEKMQLAYNARNTQRAIGTRLSSMVTRKFGMFGLQPGHITIRLRGTAGQSLGAFAVQGIKLEVMGDANDYVGKGLSGGTIVVRPTTSSPLETNKNTIIGNTVLYGATAGKLFAAGQAGERFAVRNSGATVVVEGCGSNGCEYMTGGTAVILGRVGDNFAAGMTGGMAYVYDLDDSLPLYINDESVIFQRIEVGHYESQLKHLIEEHVTETQSRFAAEILNDWAREVTKFWQVVPKEMLNRLEVPVH 1507
BLAST of glutamate synthase vs. SwissProt
Match: gi|2506463|sp|P55038.2|GLTS_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 2; AltName: Full=FD-GOGAT) HSP 1 Score: 1140.56 bits (2949), Expect = 0.000e+0 Identity = 623/1519 (41.01%), Postives = 900/1519 (59.25%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKK--SFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWI--EKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEE--EGIEAAL-NRINNEVEMIAHDSNIICLSDIE----VDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEFLM--------------ASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQR-------------NDFSTYKLFAK-----------------------EIDKV-----------------SRNA------TMNRIGGKSNSGEGGEDEFRFKSL------------------ENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEH----VLDRELIMQTTLARSNNSEV--NRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVAD-EVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELK 1401
+ +L+ P +V+ AL L MEHRG A+ ++GDGAG++ IP + + +P GVGM F P++P + ++ + + + L + + REVPV++ ++G A ++P +EQ V + +L+ +L+ RS I L +DFY+ SFS +TIVYKG + ++ L ++LDL++ +TS A+ H RFSTNT+PKW LAQP R + HNGEINT+ GNINW AREK+L +S ++L + PI + SDS N D+ E L+R+GRS A M+++PEA++N +L Y + F++Y+ + EPWDGPA + F+DG IVGA LDRNGLRP+RY IT+DD IVLGSEAG + + IV+K RL PG+M+ +DL E++I+ + ++K ++YPY +WI ++QTV D + D +++ Q AFG++ ED ++++ MA QGKEP MG D PLA+LS + +YFKQ+FAQVTNPPID LRE MSL +LG G +++ + A ++L SP L EV L+ + + VS + + +E AL N + + + + I+ L+D + E+ +P LLA+G+VHH +I V+ W THHFA L+ YGA AI PYLA +S + + W +E + LM +Y ++ E GL KI+SK+G+S + SY GAQ FEA+G+ +V F GT SRVGG+ E+ H +AF ++ + L + G ++ GE+H+ +P+ L AV + + Y+ + K E++ V SR A MNR+G KSNSGEGGED R+ +L +NGD+ SAIKQ+ASGRFGVT YL + K+++IKMAQGAKPGEGGQLPG KV + IA +R S PGV LISPPPHHDIYSIEDLAQLI+DL N EA++SVKLV+++G+G IA+GVAKA+AD I ISG DGGTGASPLSSI+H G PWELG++E H+ L++N+LRDRV+L+ADG ++TG D+ +A ++GAEE+G + A++ EGCIM R CH N CPVG+ATQ + LR++F G +VN+F+F+A +R ++A +G S++D++GRTDLL++ + KT++L L+ LL P + Q Q NHE H VLD +++ + + N + + +++ +T+R VGT LS IAK YG+ G + ++ ++F+G+AGQSFGAF G++L L+GEANDYV KG++G EI I+P + FA N+I+GN LYGAT G L G AG+RF VRNS AV+EG G + CEYMTGG ++VLG +G N GAGM+GG A+ ++ IN E++++ + + E L+ I E T S IL +W F + P K
Sbjct: 41 FIANLRGKPDHTLVEQALKALGCMEHRGGCSADNDSGDGAGVMTAIPRELLAQWFNTRNLPMPDGDRLGVGMVFLPQEPSAREVARAYVEEVVRLEKLTVLGWREVPVNSDVLGIQAKNNQPHIEQILVTCPEGCAGDELDRRLYIARSII-------GKKLAEDFYVCSFSCRTIVYKGMVRSIILGEFYLDLKNPGYTSNFAVYHRRFSTNTMPKWPLAQPMRLLGHNGEINTLLGNINWMAAREKELEVSGWTKAELEALTPIVNQANSDSYNLDSALELLVRTGRSPLEAAMILVPEAYKNQPALKDYPEISDFHDYYSGLQEPWDGPALLVFSDGKIVGAGLDRNGLRPARYCITKDDYIVLGSEAGVVDLPEVDIVEKGRLAPGQMIAVDLAEQKILKNYQIKQQAAQKYPYGEWIKIQRQTVASDSFAE---KTLFNDAQTVLQQQAAFGYTAEDVEMVVVPMASQGKEPTFCMGDDTPLAVLSHKPRLLYDYFKQRFAQVTNPPIDPLRENLVMSLAMFLGKRGNLLEPKAESARTIKLRSP-LVNEV--ELQAIKTGQLQVAEVSTLYDLDGVNSLETALDNLVKTAIATVQAGAEILVLTDRPNGAILTENQSFIPPLLAVGAVHHHLIRAGLRLKASLIVDTAQCWSTHHFACLVGYGASAICPYLALES---VRQWWLDEKTQKLMENGRLDRIDLPTALKNYRQSVEAGLFKILSKMGISLLASYHGAQIFEAIGLGAELVEYAFAGTTSRVGGLTIADVAGEVMVFHGMAFPEMAKK-LENFGFVNYRPGGEYHMNSPEMSKSLHKAVAAYKVGGNGNNGEAYDHYELYRQYLKDRPVTALRDLLDFNADQPAISLEEVESVESIVKRFCTGGMSLGALSREAHETLAIAMNRLGAKSNSGEGGEDVVRYLTLDDVDSEGNSPTLPHLHGLQNGDTANSAIKQIASGRFGVTPEYLMSGKQLEIKMAQGAKPGEGGQLPGKKVSEYIAMLRRSKPGVTLISPPPHHDIYSIEDLAQLIYDLHQINPEAQVSVKLVAEIGIGTIAAGVAKANADIIQISGHDGGTGASPLSSIKHAGSPWELGVTEVHRVLMENQLRDRVLLRADGGLKTGWDVVMAALMGAEEYGFGSIAMIAEGCIMARVCHTNNCPVGVATQQERLRQRFKGVPGQVVNFFYFIAEEVRSLLAHLGYRSLDDIIGRTDLLKVRSDVQLSKTQNLTLDCLLNL--PDTKQN---RQWLNHEPVHSNGPVLDDDILADPDIQEAINHQTTATKTYRLVNTDRTVGTRLSGAIAKKYGNNGFEG-NITLNFQGAAGQSFGAFNLDGMTLHLQGEANDYVGKGMNGGEIVIVPHPQASFAPEDNVIIGNTCLYGATGGNLYANGRAGERFAVRNSVGKAVIEGAGDHCCEYMTGGVIVVLGPVGRNVGAGMTGGLAYFLDEVGDLPEKINPEIITLQRITASKGEEQLKSLITAHVEHTGSPKGKAILANWSDYLGKFWQAVPPSEK 1536
BLAST of glutamate synthase vs. nr
Match: gi|503529513|ref|WP_013763635.1| (glutamate synthase large subunit [Haliscomenobacter hydrossis] >gi|332331979|gb|AEE49080.1| Glutamate synthase (ferredoxin) [Haliscomenobacter hydrossis DSM 1100]) HSP 1 Score: 1605.5 bits (4156), Expect = 0.000e+0 Identity = 788/1475 (53.42%), Postives = 1036/1475 (70.24%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFK---EEEGIEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDE---EFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGD--LRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAK 1407
L+ +L I + +V+ ALT+L NMEHRGA G E NTGDGAGIL+Q PH F K +I++P YGVGM FFP D ++ C+ + + Y+ + R+VP D+ ++G A+ +EP +EQ FVK K LE +L+ LR ++++ Y+ + ++FYI+SFS KT+VYKGQL+ QLR YFLDLQD SA+A++HSRFSTNTVPKWKLAQPFR I+HNGEINT++GN+NWW+++EK L D +L + PIC +SDSGNFDNV E+L+ SG SLPHALMMMIPEAW++DD++ YKK+FYEYH IMEPWDGPASICFT+G +VGA LDRNGLRPSRY + ED+ +++ SEAG L +D S++VKK RL+PG+ML+ DLDE RI+ DEE+KSTIC+R PY+DW+++ + ++DL + P E+D + RNQ FGF++ED +++ +G+EP+GSMGAD PLA+LS +QH SNYFKQ FAQVTNPPID +RE+ MSL LGG+ K +D+ + + L+SP++T +R++YL+ S F + + F+ +E ++AA++ + + E ++ NI+ LSD + DE + +P+LLAIG+VHH +I R VE GDI ETHHF +L+ YGA + PY+ Y + H + E ++ ++ K K +LK+MSK G+ST+ SY+GAQ FE LG+S VV KCF G++SR+ G+ + G +E+ A+H +AF + L L G++QWKR+GE HLFNP+SIH LQ A ++NDF YK +A I++ + A MNR+GGKSNSGEGGEDE RF+ ENGD SAIKQVASGRFGVT YLTNA E+QIKMAQGAKPGEGGQLPGHKVD+ I RVR+STPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN ARI+VKLVSK GVG+IASGVAKAHAD ILISG DGGTGASPL+SIRH GLPWELGL+E HQTL++NKLRDRVV+Q DGQ+RTG+DIAIAT+LGAEEWG+ATAALVVEGCIMMRKCH+NTCPVGIATQD ELRK+FNGK EH++N+F FLA LR IMA++G ++N++VG+ +LL + + HWK R ++L A+L+K + Y +Q+H + VLDR+LI Q LA N +V+ +F I++T+RAVG MLSNE++K Y KG+ +++ FRGSAGQSFGAFAA GL LEGEANDY KGLSG + ++PDR +KF +NII+GNVA YGAT+GE I+GMAG+RFGVRNSG AVVEG+G +GCEYMTGG V+VLG G NF AGMSGG A++++ E + +N EMV +D +E D LR ++ + T+S VA +L +W++ + F K+ P++ KA+L +
Sbjct: 22 LMANLNGIKSHELVEDALTMLANMEHRGACGCEPNTGDGAGILIQTPHEFLKKKCRELKIDLPDFGEYGVGMVFFPMDRAQRQECRVLFQDYMDECGFELIGWRKVPTDHELLGESAVATEPRVEQVFVKPKKTMDLKTLERRLYILRKFVIHNIYKVFPHVKENFYIASFSYKTVVYKGQLTTYQLRPYFLDLQDADLKSAIAVIHSRFSTNTVPKWKLAQPFRYIAHNGEINTVRGNLNWWQSKEKNLMSEIFSDEELELIKPICGDDLSDSGNFDNVLEYLVMSGYSLPHALMMMIPEAWEHDDTMEDYKKAFYEYHKTIMEPWDGPASICFTNGILVGATLDRNGLRPSRYCLLEDNTLIVASEAGALPVDQSKVVKKGRLQPGRMLIADLDEGRIVGDEELKSTICQRLPYRDWMKEYRLTLEDLPENIPGVVELDSKTLHRNQQLFGFTKEDIKVLINPTVAEGQEPLGSMGADTPLAVLSHQSQHLSNYFKQLFAQVTNPPIDPIRERSVMSLFAMLGGNPKHLDLVPERGRFIHLESPVITNGELNRVKYLEHSYFKSGVIDILFRADGQEGRLKAAIDNLCAQAEDLVRQGCNILILSDRQADEKHAPMPSLLAIGAVHHHLIRRGLRTLTSLVVEAGDIRETHHFCSLIGYGATCVNPYMVYATIAEQRELGHLDASMKLEKMIDTFNKVIGKSILKVMSKNGISTLQSYQGAQIFEILGISNEVVEKCFTGSVSRIQGISYDGIAEEVLARHTIAFPEMPLPGEQLEVGGVYQWKRRGEAHLFNPKSIHHLQVASRKNDFQEYKKYASIINEQTEQALTLRGLLTFRKGNPIPIEEVEPVENIMKRFATGAMSFGSISHEAHSTLAIAMNRMGGKSNSGEGGEDEIRFEPKENGDWERSAIKQVASGRFGVTSYYLTNAAELQIKMAQGAKPGEGGQLPGHKVDEWIGRVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANPAARINVKLVSKAGVGIIASGVAKAHADAILISGHDGGTGASPLTSIRHAGLPWELGLAETHQTLLRNKLRDRVVVQTDGQLRTGKDIAIATLLGAEEWGVATAALVVEGCIMMRKCHVNTCPVGIATQDPELRKRFNGKPEHVINFFRFLAEDLRSIMAELGFRTINEMVGKVELLRLRSNVQHWKYRQVDLNAILFKQPVDENVGQYKRVEQDHGIAGVLDRKLIYQAKLALENGQKVSSIFPIQNTDRAVGAMLSNEVSKRYKGKGLPDATIDFRFRGSAGQSFGAFAAPGLQFTLEGEANDYFGKGLSGGRLIVVPDREAKFEPQENIIIGNVAFYGATAGEAYIKGMAGERFGVRNSGVKAVVEGVGDHGCEYMTGGVVVVLGETGKNFAAGMSGGIAYVHNPLGEFEKRVNLEMVDLDPMEDEDFELLRRMLRNHFSCTSSKVAREMLENWDIQRQFFTKVMPRDYKAVLAKR 1496
BLAST of glutamate synthase vs. nr
Match: gi|763358051|ref|WP_044215606.1| (glutamate synthase large subunit [Phaeodactylibacter xiamenensis]) HSP 1 Score: 1592.4 bits (4122), Expect = 0.000e+0 Identity = 794/1476 (53.79%), Postives = 1024/1476 (69.38%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFK--EEEG-IEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEFL---MASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGD-LRQNVLPDI-GLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDK-------------------------------VSRNAT-------------------MNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILK 1405
L+ +L I + +++ ALT+L NMEHRGA G E NTGDGAGIL+Q PH FF +P YGVG+ FFP D + C+ + YI + R++P D+ +G A+ EP MEQ FV K + LE +L+ LR + +++TY Q D FY++SFS KT+VYKGQL+ QLR YF DL D+ SA+A++HSRFSTNTVPKWKLAQPFR I+HNGEINTI GN+NWW ++E L + + +L + PIC +SDS NFDNV EFL+ G SLPHALM+MIPEAWQ+D+ + YK++FYEY+ IMEPWDGPASICFTDG ++GA LDRNGLRPSRY +TED+++++ SEAG L +D S++V K RL+PGK+L+ DLDE R+I D E+K IC+R PY++W+++ V + DL P + E D + ++RNQ GF++ED +++ M GK+P+GSMGAD PLA+LSK +QH +NYFKQ FAQVTNPPID +RE+ MSL LGGS I+D+ D A + LDSP+LT E +++Y++ +F + + FK ++EG ++AA++ + E ++ + SNI+ LSD + +P+LLA+G+VHH +I + VE GD+ E HH+ATL+ YGA AI PY+AY S + K ED + L + +Y KA KGLLKIMSK+G+ST+ SY+GAQ FE LG+++ VV KCF+G+ISR+ G+ F G +E +HR+AF + QN ++ G++QWKR+GE HLF+PQ+IHLLQ A + D+ TYK +A+ ID ++R AT MNRIG KSNSGEGGEDE RF+ ENGD SAIKQVASGRFGVT +YLTNA E+QIKMAQGAKPGEGGQLPGHKVDD I RVR+STPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN++ARI+VKLVSK GVG+IASGVAKAHAD ILISG DGGTGASPL+SIRH GLPWELGL+E HQTLVKNKLRDRV LQ DGQMRTGRD+AIAT+LGAEEWG+ATAALVVEGCIMMRKCH+NTCPVGIATQD ELRK+F GK EH++N+F FLA LR IMA +G +++++VG+ ++L+ N +WK R L+L +LYK + + + +Q+H + HVLDR L+ A + + V F I +T+RA GTMLSNEI+K +G KG+ + ++ HFRGSAGQSF AF A GLS LEGEANDY KGLSGA++ +PDR++KF + NII+GNVA YGATSGE I GMAG+RF VRNSG VVEGIG +GCEYMTGG V+VLG G NF AGMSGG A+IY+ + +N M+ +D +E D L I K ++LT S A +L DWEL + F K+ P++ K +L+
Sbjct: 23 LIANLNGIKSHKVIEDALTMLTNMEHRGACGCEPNTGDGAGILIQTPHAFFAKKCKEQGFELPEFGKYGVGVVFFPADRNLRRQCRVLFDDYIDELGFDLLGYRKIPTDHEELGESAVSVEPRMEQVFVAPKTPMERKALERRLYVLRKFSTHTIHNTYPQSK---DQFYLASFSYKTVVYKGQLTTYQLRPYFPDLHDELCESAIALIHSRFSTNTVPKWKLAQPFRYIAHNGEINTIMGNLNWWASKEGLLKSTLFTEEELEKLKPICGESLSDSANFDNVLEFLVLGGMSLPHALMLMIPEAWQHDEQMEDYKRAFYEYNRTIMEPWDGPASICFTDGILLGATLDRNGLRPSRYCLTEDNVLIVASEAGALPVDQSKVVTKGRLQPGKILIADLDEHRVIGDTELKDVICQRLPYREWLDQNRVSLKDLPVQQPAKVEFDQNTLLRNQQMHGFTKEDLKVVIGPMIKAGKDPLGSMGADTPLAVLSKQSQHLANYFKQLFAQVTNPPIDPIRERSVMSLYAMLGGSSNILDINADRAKFIHLDSPLLTNEDLSKVKYINHPDFKSGVIDIVFKAGKQEGLLQAAIDHVCGVAEDLVRNGSNILILSDRNAGPYQAPIPSLLAVGAVHHHLIQKGLRSLTSIVVEAGDVREAHHYATLIGYGASAINPYMAYASIAQLYGKGVFEDGKTLDEVINTYNKAIGKGLLKIMSKIGISTLQSYQGAQIFEVLGLNKEVVEKCFRGSISRIQGIGFDGIAEEAMVRHRMAFPENGGQNPRLEVGGVYQWKRRGEAHLFSPQAIHLLQFATRTGDYKTYKKYARLIDDQAEQALTLRGLLTFRRGEKVPLKEVEPAESIMTRFATGAMSFGSISHEAHATLAVAMNRIGAKSNSGEGGEDEVRFERKENGDWERSAIKQVASGRFGVTSHYLTNADELQIKMAQGAKPGEGGQLPGHKVDDWIGRVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANRKARINVKLVSKAGVGIIASGVAKAHADAILISGHDGGTGASPLTSIRHAGLPWELGLAETHQTLVKNKLRDRVTLQTDGQMRTGRDLAIATLLGAEEWGVATAALVVEGCIMMRKCHMNTCPVGIATQDPELRKRFTGKPEHVINFFRFLAEDLREIMASLGFRTLDEMVGKVEMLKAKNKPTYWKYRGLDLSPILYKEPADENVGQHKAVEQDHGIAHVLDRRLMATAQAAINQGNRVEASFDIRNTDRATGTMLSNEISKRHGGKGLPEGTIQFHFRGSAGQSFAAFGAPGLSFRLEGEANDYFGKGLSGAQLIAVPDRDAKFTASDNIIIGNVAFYGATSGEAYINGMAGERFAVRNSGVKTVVEGIGDHGCEYMTGGIVVVLGDTGKNFAAGMSGGIAYIYNPDGHFEQRVNRGMIDLDPMEAEDVSRLHALITKHHKLTGSTRAATLLADWELAQQQFTKVMPRDYKRVLE 1495
BLAST of glutamate synthase vs. nr
Match: gi|1269685730|ref|WP_099148121.1| (glutamate synthase large subunit [Lewinella nigricans] >gi|1269200918|gb|PHN08530.1| glutamate synthase large subunit [Lewinella nigricans DSM 23189 = NBRC 102662]) HSP 1 Score: 1567.75 bits (4058), Expect = 0.000e+0 Identity = 787/1478 (53.25%), Postives = 1015/1478 (68.67%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEE-EG--IEAALNRINNEVEMIAHD-SNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEF----LMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDI---GLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKD 1408
+ +L + +V AL +L NMEHRGA G E NTGDGAGIL Q+PH F + +N+P YGVGM FFP+ + + + ++ YI + R + N IG A+ EP MEQ FV+ KD LE +L+ LR ++ + D+FYI+SFS KTIVYKGQL+ Q++ YF DLQD + SA+A+VHSRFSTNTVPKWKLAQPFR ++HNGEINTIQGN+NWW+A+EK + + +L + PIC ++SDSGNFDNV EFL+ SG SLPHALMMMIPEAWQ+DD + YKK+FYEYH IMEPWDGPASICFTDG +VGA LDRNGLRPSRY +T+D+++++ SEAG + ID S++VKK RL+PGK+L+ DLDE RII DEE+KS IC+R PY++W+++ + +DD++ G E+D + RNQ FGF++ED ++L M +GK+PIGSMGAD PLA+LS +QH NYFKQ FAQVTNPPID +RE+ MSL LGGS I+D G + A+ + LD P+L+ +++ L F + F + +G + AA++ I E + + +NI+ LSD + + +P+LLA+G+VHH +I VE GD ETHHFATL+ YGA AI PY+AY S + R+ +++ L+ +Y KA KGLLKIMSK+G+ST+ SY+GAQ FE LG+++ VV CF G++SR+ G+ + G KE+ +H LAF PD+ G++QWKR+GE HLFNPQSIHLLQ A ++ D++ +K ++K I+ + A MN+IGG+SNSGEGGED RF ENGD SAIKQVASGRFGVT YL NA E+QIKMAQGAKPGEGGQLPGHKVDD I RVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN +ARI+VKLVSK GVG+IASGVAKAHAD ILI+G DGGTGASPL+SIRH GLPWELGL+E+HQTLVKNKLRDRVVLQ DGQ+RTGRD+AIAT+LGAEEWG+ATAALVVEGCIMMRKCHLNTCPVGIATQD ELRK+F G EH++N+F FLA LR IMA++G +V ++VG+ +LL++ ++WK + L+L +LYK + +Q+H + VLDR LI A ++ VN FKI++T+RAVG MLS+EI++ + ++G+ ++ FRGSAGQSFGAF A GL LEGEANDY KGLSG + ++PDR + F H+NII+GNVA YGATSGE I+G AG+RF VRNSG AVVEGIG +GCEYMTGG V+VLG G NF AGMSGG A++Y+ + + +N EM+ +D L+ AD LR I+ + T+S AL+IL +W K F+K+ P++ KA+L+ ++
Sbjct: 23 FIANLNGHKSHELVADALQMLTNMEHRGACGCEPNTGDGAGILTQVPHDFLTIECKKIGLNLPEYGAYGVGMVFFPQHEVLRRQSRFLLNDYIDECGFDLIGYRPLKTINDDIGPSAMSVEPRMEQVFVRPKDKLDLKTLERRLYILRKYATHNIHLTYPQSKDEFYITSFSYKTIVYKGQLTTYQVQPYFADLQDPNYKSAIALVHSRFSTNTVPKWKLAQPFRYVAHNGEINTIQGNVNWWKAKEKLIKAELFSEEELEKLKPICGQHLSDSGNFDNVLEFLVLSGISLPHALMMMIPEAWQHDDHMEDYKKAFYEYHKTIMEPWDGPASICFTDGILVGATLDRNGLRPSRYCLTDDNVLIVASEAGVIPIDQSKVVKKGRLQPGKILIADLDEHRIIGDEELKSIICQRLPYREWLDEHRLTLDDIQERGNPVIELDEKTLFRNQQLFGFTKEDLRVLLAPMIKEGKDPIGSMGADTPLAVLSHHSQHLGNYFKQLFAQVTNPPIDPIRERSVMSLYAMLGGSRNILDRGPERASFIHLDQPLLSNGELKKIKELHHPRFRSGVIDTLFDADGQGGRLRAAIDHICAVAEDLTRNGANILILSDRNANAYRAPLPSLLAVGAVHHHLIRTGLRALTSIVVETGDARETHHFATLIGYGASAINPYMAYASIADLRRRGGVFEDDLSLDKLLYTYKKAVGKGLLKIMSKIGISTLQSYQGAQIFEILGLNKEVVDHCFAGSVSRIEGIGYDGIAKEVLVRHHLAFPH-SSTAAPDLEVGGIYQWKRRGEAHLFNPQSIHLLQFATRKKDYTAFKKYSKIINDQAEQALTLRGLLTFRKGEAIDISEVEPVEKILKRFATGAMSFGSISHEAHSTLAIAMNKIGGRSNSGEGGEDPIRFDRKENGDWERSAIKQVASGRFGVTSYYLNNAIELQIKMAQGAKPGEGGQLPGHKVDDWIGRVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANPDARINVKLVSKAGVGIIASGVAKAHADAILIAGHDGGTGASPLTSIRHAGLPWELGLAESHQTLVKNKLRDRVVLQTDGQIRTGRDLAIATLLGAEEWGVATAALVVEGCIMMRKCHLNTCPVGIATQDPELRKRFTGDPEHVINFFTFLAQELREIMAELGFRTVEEMVGKVELLKVKQHIDYWKYQALDLSPILYKEPADPSVGQFKRVEQDHGIADVLDRRLIEHAQPALNDAQVVNTTFKIKNTDRAVGAMLSSEISRRFHAEGLPDATIQYRFRGSAGQSFGAFGAKGLEFTLEGEANDYFGKGLSGGRLIVVPDREATFDPHENIIIGNVAFYGATSGEAYIKGTAGERFCVRNSGVKAVVEGIGDHGCEYMTGGVVVVLGETGKNFAAGMSGGMAYVYNPEKKFESLVNQEMIDLDPLDEADFELLRRMIRNHFSYTSSKTALDILDNWNESKKLFVKVMPRDYKAVLRRRN 1499
BLAST of glutamate synthase vs. nr
Match: gi|522023735|ref|WP_020534950.1| (glutamate synthase large subunit [Lewinella cohaerens]) HSP 1 Score: 1558.12 bits (4033), Expect = 0.000e+0 Identity = 777/1478 (52.57%), Postives = 1002/1478 (67.79%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGDLENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFK---EEEGIEAALNRINNEVE-MIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARK---WHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDI-----GLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAK 1407
L+ +L +PT ++ ALT+L NMEHRGA G E N+GDGAGIL+Q+PH F + +P YGVGM FFP D I + C+ + YI + + R+V D+ +G + +EP MEQ FVK K + LE +LF LR ++ +Q A D FY++SFS KTIVYKGQL+ QLR YF DLQD R SA+A++HSRFSTNTVPKWKLAQPFR I+HNGEINT++GN+NWW ++E L ++ ++ +LP+C ++SDSGNFDNV EFL+ +G SLPHALMMMIPEAWQND+ +P +KK+FYE+H +MEPWDGPASICFTDG +VGA LDRNGLRPSRY +T DD +++ SEAG L ID S++V K RL+PG++L+ DL E RII D+E+K TIC+R PY++W++ + + DL + + + R Q G ++ED +I++ M GK+PIGSMGAD+PLA LS+ +QH SNYFKQ FAQVTNPPID +RE+ MSL LGG I+DV + A + LD P+L+ + R++ L S++ ++ FK + + AA++ I E ++ + I+ +SD + D + +P+LL++G++HH ++ VE GD ETHHFATL+ YGA A+ PYL Y + + +K + ++ + Y KA KGLLKIMSK+GVST+ SY+GAQ FEALG+ + VV CF GTISR+ G+ F G +E +H LA+ + LP+ G++QWKR+GE H FNP+SIH LQ A + ND+ YK +A EI+ + A MNRIGG+SNSGEGGEDE RF ENGDSM SA KQ+ASGRFGVT +YL+ A E+QIKMAQGAKPGEGGQLPG KVDD I RVR+STPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+EARI+VKLVSK GVG+IASGVAKAHAD ILISG DGGTGASPLSSIRH GLPWELGL+E+HQTLVKNKLRDRV LQ DGQ+RTGRD+AIAT+LGAEEWG+ATAALVVEGCIMMRKCH+NTCPVGIATQDKELRK+F GK EH++N+F FLA LR IMA++G ++N++VG + L++ +WK RDL+L LLYK G + Y Q+H ++ +LDR+L+ A V F I++T+RAVGTMLS EI+K++ G+ S+ FRGSAGQSFGAF A G+ VLEGEANDY KGLSG + ++PDR + F KNII+GNVA YGAT GE I+G+AG+RF VRNSG VVEGIG +GCEYMTGG V+ LG G NF AGMSGG A++Y + + +N EMV ++ L D+ R ++ + T+S AL++L DWE F K+ P++ KA+L+ K
Sbjct: 33 LIANLSGVPTHDLIKDALTMLVNMEHRGACGCEPNSGDGAGILIQLPHDFLVDKCQENGFTLPAFGEYGVGMVFFPADKILSDQCRFLFDDYIDELGLELLGYRKVTTDHEEVGPTSRSTEPRMEQVFVKPKTEMEPDALERRLFVLRKYATHNIHQTYPATKDQFYLASFSYKTIVYKGQLTTWQLRPYFPDLQDIRCKSAIALIHSRFSTNTVPKWKLAQPFRMIAHNGEINTVRGNLNWWRSKESLLKSNKFSQEEMDKLLPVCGEHLSDSGNFDNVLEFLVLNGFSLPHALMMMIPEAWQNDELMPDHKKAFYEFHRTMMEPWDGPASICFTDGILVGATLDRNGLRPSRYCLTNDDRLIVASEAGALVIDQSKVVMKGRLQPGRVLIADLKEHRIIGDQELKDTICRRLPYREWLDANKITLKDLPLEESLGTSLHKNTLFRLQQLHGITKEDLKVIIEPMISLGKDPIGSMGADIPLACLSQQSQHLSNYFKQLFAQVTNPPIDPIRERSVMSLYAMLGGEDNILDVKPERARYIHLDHPLLSNDDLGRIKQLHHSHYKAGTINAIFKIGGQSGRLRAAVDHICAMAEDLVRNGHGILVISDRQADAFHAPIPSLLSVGAIHHHLVRLGLRSQTSLVVEAGDARETHHFATLIGYGASAVNPYLTYATISDLYQKGAFGKEKSRDYYIEVYNKAIGKGLLKIMSKIGVSTLQSYQGAQIFEALGIHRDVVDHCFTGTISRIQGVGFDGIARESLVRHDLAY---PKQALPNPQLEVGGVYQWKRRGEEHTFNPKSIHYLQLAARTNDYDAYKKYATEINHQTEKALTLRGLLAFRKGDPIPIEQVEPAEAIMKRFATGAMSFGSISWEAHTTLAIAMNRIGGRSNSGEGGEDEARFTPDENGDSMNSATKQIASGRFGVTSHYLSQANELQIKMAQGAKPGEGGQLPGGKVDDWIGRVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSKAGVGIIASGVAKAHADAILISGYDGGTGASPLSSIRHAGLPWELGLAESHQTLVKNKLRDRVTLQTDGQLRTGRDLAIATLLGAEEWGVATAALVVEGCIMMRKCHVNTCPVGIATQDKELRKRFTGKPEHVINFFRFLAEDLREIMAELGFRTINEMVGHVERLKVNREVTYWKYRDLDLTPLLYKEAVGEEVGQYKKVVQDHGIDQILDRQLVAHAQPALLAAQHVKTTFPIKNTDRAVGTMLSYEISKIWKGDGLPDASIEYRFRGSAGQSFGAFGARGIEFVLEGEANDYFGKGLSGGRLIVVPDREATFDPAKNIIVGNVAFYGATDGESYIKGLAGERFAVRNSGVKTVVEGIGDHGCEYMTGGLVVNLGETGKNFAAGMSGGMAYVYDPKKDFAERLNAEMVELEPLNEDDQELFRQMLRNHFAYTSSKTALDMLNDWEATLKVFTKVMPRDYKAVLERK 1507
BLAST of glutamate synthase vs. nr
Match: gi|1053211299|ref|WP_065999878.1| (glutamate synthase large subunit [Mucilaginibacter sp. PPCGB 2223] >gi|1052496933|gb|OCX53216.1| glutamate synthase subunit alpha [Mucilaginibacter sp. PPCGB 2223]) HSP 1 Score: 1536.16 bits (3976), Expect = 0.000e+0 Identity = 760/1471 (51.67%), Postives = 997/1471 (67.78%), Query Frame = 0
Query: 13 IVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNGD-LENQLFFLRSSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCL-SIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFK---EEEGIEAALNRINNEVEMIAHDS-NIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVI---ARKWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLR--QNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAK-------------------------EIDKV-------SRNAT-------------------MNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSVV 1412
I+ ALTILENMEHRGA G + TGDGAGIL+Q+PH FF EI++P YGVGM FFPKDP K C++II+ + ++ + R+VPVDN+ IG A +EPD+EQ F+ H N D E +L+ LR I + + DFY +S S KTI+YKGQL+ Q+R YF DL D R SA ++HSRFSTNT P W+LAQPFR I+HNGEINT+ GN+NW+ + K L S ++ +LP+ D SDS DN+ E LL +GRSLPH +MM+IPEAW ++ + KK+FYEYH +MEPWDGPA+I FTDG ++GA LDRNGLRP RY+IT+D+ ++ SEAG L S+ S +V+K RL+PGKM +ID ++ +IIAD+E+K I R PY W+E + +++L + D++ R Q FG+SRED + I+ MA+ GKEP+GSMG DVPLA+LS QH S+YFKQ FAQVTNPPID +RE+ MSL T++G +G ++D + + L PILT ++L +D F + FK + +E + R+ E D ++ L D VD + +P+L+A+ +VHH ++ + VE GDIWE HHFA LL++GA AI PYLA + + + + D++ L +Y KA GLLKI SK+G+ST+ SY G+Q FE LG++Q VV +CF G ++R+GG+ +E KHR+ FG + ++LP+ G++QWKR+GE HLFNPQ+IHLLQHA + ND++ YK +AK ID+V R AT MNRIG KSN+GEGGEDE R+ LENGDSM SAIKQ+AS RFGVT NYLTNA E+QIKMAQGAKPGEGGQLPGHKVD+ IA+ R++TPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+ ARI+VKLVSK GVG IA+GVAKAHAD ILI+G DGGTGASP+SSI+H GLPWELGL+EAHQTLV+NKLR RVVLQADGQM+TGRD+AIA ++GAEEWG+ATAALV GCIMMRKCHLNTCPVG+ATQD+ELRK F+GK EH+VN F FLA +R IMA++G ++N++VGR L++ HWK + ++L +L+ T+Y S+KQ+H ++ +LD +L+ Q A + S V F++++T+R +GTMLSNEI+K+YGS G+ + ++N F GSAGQSFGAF+ GLS LEGEANDYV KGLSGA++ I P + + F +NII+GNVALYGATSGE+ IRGMAG+RF VRNSGA AVVEG+G +GCEYMTGG ++LGS G NF AGMSGG A++Y + N N EMV +D L D+ + ++K ++T S VA ++L +WE +L F+K+FP+E K +L+ + +
Sbjct: 37 IILDALTILENMEHRGACGCDPLTGDGAGILIQLPHEFFMDECAALEISLPEPGEYGVGMTFFPKDPTVKKACRNIIKNAVEKLGLIFLGYRKVPVDNSGIGETAKAAEPDVEQLFIGRPHHITNADDFERKLYVLRRFITKTVNETLKDAVADFYFTSLSCKTIIYKGQLTTYQVRQYFNDLSDPRMESAFGMIHSRFSTNTFPSWRLAQPFRMIAHNGEINTLTGNLNWFYSGVKSLASSYFSQEEMEMLLPVIDNNQSDSACLDNIIEILLHTGRSLPHVMMMLIPEAWDGNEQMDPLKKAFYEYHATLMEPWDGPAAISFTDGKLIGALLDRNGLRPLRYVITKDNRVIAASEAGVLASLRESDVVRKGRLQPGKMFLIDTEQGKIIADDEIKRKISSRQPYGRWLENYKIRLEELAEPRLSFSSLSADSVFRYQQVFGYSREDIETIIMPMALDGKEPVGSMGTDVPLAVLSDKPQHLSSYFKQFFAQVTNPPIDPIRERLVMSLATFIGSNGNLLDEDKMHCHCVALKQPILTNYELEKLRSIDTGLFHAKTLQTYFKADGQPGSLEKGIARLCRYAEDAVEDGFEVLILQDRAVDSEHAPIPSLMAVSAVHHHLVRKGLRGSVGLVVEAGDIWEVHHFACLLAFGATAINPYLALATIDTLKAEGKLGASLDQKALQKNYVKAVSDGLLKIFSKMGISTLQSYHGSQVFEILGLNQDVVDRCFAGAVTRIGGLGLDEIAREALCKHRIGFGASKDASHLLPEGGIYQWKRRGEQHLFNPQTIHLLQHATRTNDYNVYKNYAKLINDQTDKHFTIRGMLDFAHHREAISIDEVEPAEAIMKRFATGAMSFGSISHEAHSTLAIAMNRIGAKSNTGEGGEDEMRYIPLENGDSMRSAIKQIASARFGVTANYLTNADELQIKMAQGAKPGEGGQLPGHKVDEWIAKTRHATPGVGLISPPPHHDIYSIEDLAQLIFDLKNANRAARINVKLVSKAGVGTIAAGVAKAHADVILIAGYDGGTGASPISSIKHAGLPWELGLAEAHQTLVRNKLRSRVVLQADGQMKTGRDLAIACLMGAEEWGVATAALVAGGCIMMRKCHLNTCPVGVATQDEELRKLFSGKPEHIVNLFRFLAEEMREIMAELGFRTINEMVGRVQFLKVKEGLKHWKVKKVDLSGILHPVSNAKGLTLYNSEKQDHGMDEILDWKLLEQAKPALEDQSPVFGSFEVKNTDRTIGTMLSNEISKVYGSAGLPENTINYKFVGSAGQSFGAFSTKGLSFELEGEANDYVGKGLSGAQLAIYPSKEATFTPEENIIIGNVALYGATSGEVFIRGMAGERFAVRNSGATAVVEGVGDHGCEYMTGGRALILGSTGRNFAAGMSGGLAWVYDPEHTFTENCNTEMVDLDPLSAKDKEQIVTLLRKHVQITGSTVAKSLLENWEAAQLKFVKVFPREYKKVLQQAQYQTI 1507
BLAST of glutamate synthase vs. nr
Match: gi|1168001166|ref|WP_080241701.1| (glutamate synthase large subunit [Spirosoma rigui]) HSP 1 Score: 1531.92 bits (3965), Expect = 0.000e+0 Identity = 763/1484 (51.42%), Postives = 1006/1484 (67.79%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYK-DHDQNGDLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEE---EGIEAALNRINNEVEMIAHDS-NIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARK---WHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGDLRQNVLPDI---GLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSV 1411
V +K + IV AL +L MEHRGA GAE N+GDGAG+L+QIPH FF + +++P + YGVGM +FPKD + C++++ + + + + R VPV+N+ +GS + +EP MEQ F+K D D E +L+ LR + I+N+T A +D FY SS S +TI YKGQL+ +QL YF DLQ + SA+ +VHSRFSTNT P WKLAQPFR I+HNGEINT++GN+NW +A E L ++ ++ +LPICDP SDS N DN E L+ SGRSLPH +MM+IPEAW +D + +K+FYEYH A++EPWDGPASI FTDG IVGA LDRNGLRPSR+ +T+ DI+++ SE G L IDP+ +V K RL+PGKM ++D+++ RI+ADEE+K I R PYQ W+ + + DL++ D + ++R Q AFGF+ ED +IL M GKE +GSMG DVPLA+LS+ +QH S+YFKQ FAQVTNPPIDS+RE+ MSL +++G + ++ + + LD P+LT + FD+L ++D F ++ F + + +E AL+RI E D +I+ LSD +D + +P+LL+ ++HH++I R VE GD+WETHH ATL+ YGA + PY+A+++ + K + D + L +Y KA LLKI SK+G+ST+ SY+GA FE LG++ VV++ F GT+SR+GGM +E+ +H +AF D+ +P + G++QWK++GE H+FNP +IHLLQ + QRND+S +K ++K ID S+ A MNRIGGKSNSGEGGEDE R+K L NGDSM S IKQVASGRFGVT +YLTNA+E+QIKMAQGAKPGEGGQLPG KVDD I R R+STPGVGLISPPPHHDIYSIEDLAQLI DLKNAN+ ARISVKLVS+ GVG IA+GVAKAHADHILISG DGGTGASPLSSIRH GLPWELGL+EAHQTLV+NKLR RV +QADGQMRTGRD+AIA +LGAEE+G+ATAALV GCIMMRKCHLNTCPVG+ATQ+KELR F GK EH+VN F F+A LR IMA++G ++N++VG+ +LE+ + HWK +++NL+ALLYK D +Y ++QNHELEHVLDR+LI A + V F +++ +R++GTMLSNEI+K YG G+ S++I RG+AGQSFGAFA G+ L LEG+ANDY KGL GA++ + PDR ++F +N I+GNV+ YGATSGE IRGMAG+RFGVRNSGA VVEG+G +G EYMTGG I+LG G NF AGMSGG A++Y +N EMVS++TL V D +R+ ++K +E T SNVAL ++ DW+ F+K+ P + + L + S+
Sbjct: 15 FVAHIKGRKSHQIVSDALQMLRRMEHRGAVGAEANSGDGAGLLIQIPHEFFLDETRKLGVHLPPALEYGVGMVYFPKDVWLREECRAVLNRKMKRLGLELLCYRVVPVNNSDLGSGSRSAEPQMEQVFIKRPADITNPDDFERKLYILRNYSTRIINETI----AGVDTFYFSSLSCRTITYKGQLTTLQLEPYFPDLQHEDVVSALGVVHSRFSTNTFPSWKLAQPFRYIAHNGEINTVRGNVNWMKAAEGLLESAKFTKDEMDMLLPICDPKQSDSANLDNAIELLVMSGRSLPHVMMMLIPEAWDGNDQMDPVRKAFYEYHAALIEPWDGPASISFTDGRIVGATLDRNGLRPSRFWVTDQDIVIMASEVGVLDIDPATVVSKGRLQPGKMFLVDMEQGRIVADEEIKEQISSRKPYQQWLNNNKIKIQDLEAPIRTYSHYDPNKLMRMQQAFGFTSEDLRMILAPMVETGKEALGSMGTDVPLAVLSEQSQHLSHYFKQLFAQVTNPPIDSIRERAIMSLISFVGATYNLLSESPEHCRQIELDQPVLTTQEFDKLRFIDRPKFQAKTINCLFTTDGHGKSLERALDRICRYAEDAIQDGYSILVLSDRAIDSSHAPIPSLLSTSAIHHYLIHRGLRGKVGIVVEAGDVWETHHVATLIGYGASGVNPYMAFETIANMKEKGLLQVDYDLDKLYKNYVKAVNGELLKIFSKMGISTLQSYQGAMIFECLGLNTEVVNRYFTGTVSRIGGMGLDEIAREILIRHCVAFPDM-PVAIPRLEVGGIYQWKQRGEKHIFNPDTIHLLQQSTQRNDYSLFKKYSKLIDDQSQKAITLRGLLKFKKGTPVPIDEVEPIESIFKRFATGAMSFGSISWEAHTTLAIAMNRIGGKSNSGEGGEDELRYKDLPNGDSMNSGIKQVASGRFGVTSHYLTNARELQIKMAQGAKPGEGGQLPGFKVDDWIGRTRHSTPGVGLISPPPHHDIYSIEDLAQLISDLKNANRAARISVKLVSEAGVGTIAAGVAKAHADHILISGHDGGTGASPLSSIRHAGLPWELGLAEAHQTLVRNKLRGRVTVQADGQMRTGRDLAIAALLGAEEFGVATAALVAAGCIMMRKCHLNTCPVGVATQNKELRALFTGKPEHVVNMFTFIATELREIMAEMGFRTINEMVGQAQMLELRSDLPHWKYKNINLDALLYKEPTNLDVALYKQEEQNHELEHVLDRKLIELAKPALDSAEAVYGEFTVQNIDRSIGTMLSNEISKRYGGPGLPDGSIHIKLRGTAGQSFGAFATAGIKLELEGDANDYFGKGLCGAQLIVYPDRTAQFKPEENSIVGNVSFYGATSGEAYIRGMAGERFGVRNSGAKVVVEGVGDHGLEYMTGGLAIILGQTGRNFAAGMSGGVAYVYDLDGTFSSKVNMEMVSLETLNVEDHGIVREYVEKHFEYTTSNVALALMQDWDQQIGRFVKVMPADFRNALAGRGISL 1493
BLAST of glutamate synthase vs. nr
Match: gi|890836031|ref|WP_048905466.1| (glutamate synthase large subunit [Pedobacter sp. V48] >gi|576322282|gb|ETZ21966.1| glutamate synthase [Pedobacter sp. V48]) HSP 1 Score: 1525.38 bits (3948), Expect = 0.000e+0 Identity = 770/1486 (51.82%), Postives = 1007/1486 (67.77%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNG-DLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEGIEAALNR-----INNEVEMIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF--GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA---------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEI-ANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSVVLA 1414
V +K ++ I+ A+TILEN++HRGA GAE NTGDGAGI++QIPH F L+ ++ S YGVGM F PKD + C+ II + ++ + R+V + IG MAL EP+MEQ F+ G D E +L+ + S +N+T + + DFYI+SFSS+TIVYKGQL+++Q+R YF +L D R SA +VHSRF+TNT P WKLAQPFR I+HNGEINT+QGN+NW+ A K S +L +LP+ D SDSG DN+ E LL SGRSLPH LMM+IPEAW +D + K++FY++H +MEPWDGPA++ FTDG ++GA LDRNGLRP RY ITEDD +++ SEAG L++D S+I++K RL PGKM V+D+++ RII+D E+K +C PY DWI K + +++L + ++I R Q FG+SRED DLIL+ MA GKEPIGSMG D+PLAILS+ QH ++YFKQ FAQVTNPPID +REK MSL ++G +G I++ + + + PILT ++L +D F + F+ + G AL + V+ + +I LSD +D + A+P+LLA+ +VHH +I + VE GD+WE HHFATL+ +GA A+ PYLA ++ + E +E L +Y A GLLKI SK+G+ST+ SY GAQ FE LG+ +SVV F G +SR+GG+ KE KH F + +LP G ++WKRKGE HLFNPQ+IHLLQ+A ++ND+ TYK ++K +++ + A MNRIGGKSN+GEGGEDE R++ LENGDSM SAIKQ+AS RFGVT YLTNA E+QIKMAQGAKPGEGGQLPGHKVDD IA+VR++TPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+EARI+VKLVSK GVG IA+GVAKAHAD IL+SG DGGTGASPL+SI+H GLPWELGL+EAHQTLVKN+LR RVVLQ DGQ++TGRDIAIAT+LGAEEWG+ATAALV GCIMMRKCHLNTCPVG+ATQD LRK F G+ +H+VN F+FLA LR MA++G SV ++VG++D L++ A WK +DL+L A+LYK P + +++ ++ Q+H L +LD ELI A N + + F++++TNRA+GTMLSNE++K+Y S+G+ ++N F+GSAGQSFGAFA G+SL LEGEANDYV KGLSGA ++I P +K+ +NII+GNVALYGATSGEL +RG AG+RF VRNSGA AVVEG+G +GCEYMTGG V+V+G G+NF AGMSGG A+IY+ + + N EMV +D L+ DE+ + +KK +LT S+VA +L DW +FIK+FPKE KA+L K + + ++
Sbjct: 24 FVAHIKGRKSQQIISDAITILENLDHRGAVGAEINTGDGAGIMIQIPHEFLYDECLKIGFSLNESGDYGVGMLFLPKDVKAREECREIIYRAAEKLNLEVLGFRKVITNTEGIGDMALSVEPEMEQVFIARPYAIAAGADFERKLYVFKNYLSKTINNTVK---GINGDFYIASFSSRTIVYKGQLTSLQVRSYFTELSDKRVVSAFGLVHSRFATNTFPSWKLAQPFRYIAHNGEINTLQGNLNWFRASVKSFASSYFTPEELNILLPVIDESNSDSGCLDNIVELLLHSGRSLPHVLMMLIPEAWDGNDDMDELKQAFYKFHATLMEPWDGPAAVSFTDGNLIGATLDRNGLRPQRYAITEDDRVIMASEAGALALDQSKIIEKGRLTPGKMFVVDMEQGRIISDAEIKQQVCGSKPYGDWINKYQIRLEELPDPRLVFSNLSQESIFRYQQVFGYSREDIDLILKPMAQDGKEPIGSMGTDIPLAILSQKPQHLASYFKQLFAQVTNPPIDPIREKVVMSLAGFMGNNGNILEENAMQCHCVGIKHPILTNLELEKLRSIDTGVFQSKTLQTYFRAD-GNPGALAKGIERLCRYAVDAVEDGFQVIILSDRALDSEHAAIPSLLAVSAVHHHLIRKGYRGAVGIVVEAGDVWEVHHFATLIGFGATAVNPYLALETIAGFEAELQ-EKKEKLFQNYIYAVNNGLLKIFSKMGISTLQSYHGAQIFEILGLHKSVVDNYFTGAVSRIGGLGLDEIAKETLIKHNRIFKLANRPDAILPTGGNYKWKRKGEQHLFNPQTIHLLQNATRKNDYGTYKQYSKLVNEQTNQAYTIRGLFEFNYNRPAISLEEVEPVENIFKRFATGAMSFGSISHEAHSTLAIAMNRIGGKSNTGEGGEDELRYEVLENGDSMRSAIKQIASARFGVTSYYLTNADELQIKMAQGAKPGEGGQLPGHKVDDWIAKVRHATPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSKAGVGTIAAGVAKAHADVILVSGFDGGTGASPLTSIQHAGLPWELGLAEAHQTLVKNRLRSRVVLQTDGQLKTGRDIAIATLLGAEEWGVATAALVTAGCIMMRKCHLNTCPVGVATQDPNLRKLFTGEADHVVNLFYFLAQELRETMAELGFRSVEEMVGQSDALKVRAIAQEDWKQKDLDLSAILYK-APDNGLSLFKTEDQDHGLSEILDHELIKAAQPALFNKEPIYKEFEVKNTNRAIGTMLSNEVSKIYKSQGLPADTINFKFKGSAGQSFGAFATKGISLHLEGEANDYVGKGLSGARLSIFPFSTTKYVPEQNIIIGNVALYGATSGELFVRGQAGERFAVRNSGATAVVEGLGDHGCEYMTGGEVLVIGDTGSNFAAGMSGGIAWIYNVKGDFASKCNKEMVDLDPLDEQDELRITHLLKKHLQLTESSVAKFLLDDWATQSAHFIKVFPKEYKAVLMNKSNKLKIS 1503
BLAST of glutamate synthase vs. nr
Match: gi|1273039715|ref|WP_099438294.1| (glutamate synthase large subunit [Pedobacter ginsengisoli] >gi|1272250942|gb|ATP56352.1| glutamate synthase large subunit [Pedobacter ginsengisoli]) HSP 1 Score: 1524.99 bits (3947), Expect = 0.000e+0 Identity = 768/1486 (51.68%), Postives = 1007/1486 (67.77%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNG-DLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEGIEAALNR-----INNEVEMIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF--GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA---------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNH-WKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSVVLA 1414
V +K ++ I+ A+TILEN++HRGA GAE NTGDGAGI++QIPH F L+ ++ S YGVGM F PKD + C+ II + D+ + R V + IG MAL EP+MEQ F+ G D E +L+ + S +N+T + + DFYI+SFSS+TIVYKGQL+++Q+R YF +L D R SA +VHSRF+TNT P W+LAQPFR I+HNGEINT+QGN+NW+ A K S +L +LP+ D SDSG DN+ E LL SGRSLPH LMM+IPEAW +D + K++FY++H +MEPWDGPA++ FTDG ++GA LDRNGLRP RY ITEDD +++ SEAG L++D S+I++K RL PGKM V+D+++ RII+D E+K +C PY DWI K + +++L + ++I R Q FG+SRED DLIL+ MA GKEPIGSMG D+PLAILS+ QH ++YFKQ FAQVTNPPID +REK MSL ++G +G I++ + + + PILT ++L +D F + F+ + G AL + V+ + +I LSD +D + A+P+LLA+ +VHH +I + VE GD+WE HHFATL+ +GA A+ PYLA ++ + E +E L +Y A GLLKI SK+G+ST+ SY GAQ FE LG+++SVV F G +SR+GG+ KE KH F + +LP G ++WKRKGE HLFNPQ+IHLLQ+A ++ND++TYK ++K +++ + A MNRIGGKSN+GEGGEDE R++ LENGDSM SAIKQ+AS RFGVT YLTNA E+QIKMAQGAKPGEGGQLPGHKVDD IA+VR++TPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+EARI+VKLVSK GVG IA+GVAKAHAD IL+SG DGGTGASPL+SI+H GLPWELGL+EAHQTLVKN+LR RVVLQ DGQ++TGRDIAIAT+LGAEEWG+ATAALV GCIMMRKCHLNTCPVG+ATQD LRK F G+ +H+VN F+FLA LR MA++G SV ++VG++D L++ D WK +DL+L A+LYK P + ++Y ++ Q+H L +LD +LI A N + + F++++TNR++GTMLSNE++K+Y S+G+ ++N F+GSAGQSFG FA G+SL LEGEANDYV KGLSGA ++I P +K+ +NII+GNVALYGATSGEL +RG AG+RF VRNSGA AVVEG+G +GCEYMTGG V+V+G G+NF AGMSGG A+IY+ + + N EMV +D L+ DE+ + +KK +LT S+VA +L DW +FIK+FPKE KA+L K + + ++
Sbjct: 24 FVAHIKGRKSQQIISDAITILENLDHRGAVGAEINTGDGAGIMIQIPHEFLYDECLKIGFSLNESGDYGVGMLFLPKDVKAREECREIIYRAAEKLDLEVLGFRRVLTNTEGIGDMALSVEPEMEQVFIARPHAIAAGADFERKLYVFKNYLSKTINNTVK---GINGDFYIASFSSRTIVYKGQLTSLQVRSYFTELSDKRVVSAFGLVHSRFATNTFPSWRLAQPFRYIAHNGEINTLQGNLNWFRASVKSFASSYFTPEELNILLPVIDESNSDSGCLDNIVELLLHSGRSLPHVLMMLIPEAWDGNDDMDELKQAFYKFHATLMEPWDGPAAVSFTDGNLIGATLDRNGLRPQRYAITEDDRVIMASEAGALALDQSKIIEKGRLTPGKMFVVDMEQGRIISDAEIKQQVCGSKPYGDWINKYQIRLEELPDPRLVFSNLSQESIFRYQQVFGYSREDIDLILKPMAQDGKEPIGSMGTDIPLAILSQKPQHLASYFKQLFAQVTNPPIDPIREKVVMSLAGFMGNNGNILEENAMQCHCVGIKHPILTNLELEKLRSIDTGVFQSKTLQTYFRAD-GKPGALAKGIDRLCRYAVDAVEDGFQVIILSDRALDSEHAAIPSLLAVSAVHHHLIRKGYRGAVGIVVEAGDVWEVHHFATLIGFGATAVNPYLALETIAGFETELQ-EKKEKLFQNYIYAVNNGLLKIFSKMGISTLQSYHGAQIFEILGLNKSVVDNYFTGAVSRIGGLGLDEIAKETLIKHNRIFKLANRPDAILPTGGNYKWKRKGEQHLFNPQTIHLLQNATRKNDYNTYKQYSKLVNEQTNQAYTIRGLFEFNYNRPAISLEEVEPVENIFKRFATGAMSFGSISHEAHSTLAIAMNRIGGKSNTGEGGEDELRYEVLENGDSMRSAIKQIASARFGVTSYYLTNADELQIKMAQGAKPGEGGQLPGHKVDDWIAKVRHATPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSKAGVGTIAAGVAKAHADVILVSGFDGGTGASPLTSIQHAGLPWELGLAEAHQTLVKNRLRSRVVLQTDGQLKTGRDIAIATLLGAEEWGVATAALVTAGCIMMRKCHLNTCPVGVATQDPNLRKLFTGEADHVVNLFYFLAQELRETMAELGFRSVEEMVGQSDALKVRAIDQEDWKQKDLDLSAILYK-APDNGLSLYKTEDQDHGLSEILDHDLIKAAQPALFNKEPIYKEFEVKNTNRSIGTMLSNEVSKIYKSQGLPADTINFKFKGSAGQSFGGFATKGISLHLEGEANDYVGKGLSGARLSIFPFSATKYVPEQNIIIGNVALYGATSGELFVRGQAGERFAVRNSGATAVVEGLGDHGCEYMTGGEVLVIGDTGSNFAAGMSGGIAWIYNVKGDFASKCNKEMVDLDPLDEQDELRINHLLKKHLQLTESSVAKFLLDDWATQSAHFIKVFPKEYKAVLMNKSNKLKIS 1503
BLAST of glutamate synthase vs. nr
Match: gi|1057606900|ref|WP_068890768.1| (glutamate synthase large subunit [Pedobacter panaciterrae]) HSP 1 Score: 1523.84 bits (3944), Expect = 0.000e+0 Identity = 767/1486 (51.62%), Postives = 1009/1486 (67.90%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQNG-DLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFKEEEGIEAALNR-----INNEVEMIAHDSNIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKWHNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAF--GDLRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA---------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNH-WKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSVVLA 1414
V +K ++ I+ A+TILEN++HRGA GAE NTGDGAGI++QIPH F L+ ++ S YGVGM F PKD + C+ II + ++ + R+V + IG MAL EP+MEQ F+ G D E +L+ + S +N+T + + DFYI+SFSS+TIVYKGQL+++Q+R YF +L D R SA +VHSRF+TNT P W+LAQPFR I+HNGEINT+QGN+NW+ A K S +L +LP+ D SDSG DN+ E LL SGRSLPH LMM+IPEAW ++ + K++FY++H +MEPWDGPA++ FTDG ++GA LDRNGLRP RY ITEDD +++ SEAG L++D S+I++K RL PGKM V+D+++ RII+D E+K +C PY DWI K + +++L + ++I R Q FG+SRED DLIL+ MA GKEPIGSMG D+PLAILS+ QH ++YFKQ FAQVTNPPID +REK MSL ++G +G I++ + + + PILT ++L +D F + F+ + G AL + V+ + +I LSD +D + A+P+LLA+ +VHH +I + VE GD+WE HHFATL+ +GA A+ PYLA ++ + E +E L +Y A GLLKI SK+G+ST+ SY GAQ FE LG+ ++VV F G +SR+GG+ KE KH F + +LP G ++WKRKGE HLFNPQ+IHLLQ+A ++ND+STYK ++K +++ + A MNRIGGKSN+GEGGEDE R++ LENGDSM SAIKQ+AS RFGVT YLTNA E+QIKMAQGAKPGEGGQLPGHKVDD IA+VR++TPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+EARI+VKLVSK GVG IA+GVAKAHAD IL+SG DGGTGASPL+SI+H GLPWELGL+EAHQTLVKN+LR RVVLQ DGQ++TGRDIAIAT+LGAEEWG+ATAALV GCIMMRKCHLNTCPVG+ATQD LRK F G+ +H+VN F+FLA LR MA++G SV ++VG++D L++ D WK +DL+L A+LYK P + +++ ++ Q+H L +LD +LI A N + + F++++TNRA+GTMLSNE++K+Y S+G+ ++N F+GSAGQSFGAFA G+SL LEGEANDYV KGLSGA ++I P +K+ +NII+GNVALYGATSGEL +RG AG+RF VRNSGA AVVEG+G +GCEYMTGG V+V+G G+NF AGMSGG A+IY+ + + N EMV +D L+ DE+ + +KK +LT+S+VA +L DW +FIK+FPKE KA+L K + + ++
Sbjct: 24 FVAHIKGRKSQQIISDAITILENLDHRGAVGAEINTGDGAGIMIQIPHEFLYDECLKIGFSLNESGDYGVGMLFLPKDVKAREECREIIYRAAEKLNLEVLGFRKVITNTEGIGDMALSVEPEMEQVFIARPHAIAAGADFERKLYVFKNYLSKTINNTVK---GINGDFYIASFSSRTIVYKGQLTSLQVRSYFTELGDKRVVSAFGLVHSRFATNTFPSWRLAQPFRYIAHNGEINTLQGNLNWFRASVKSFASSYFTPEELNILLPVIDESNSDSGCLDNIVELLLHSGRSLPHVLMMLIPEAWDGNEDMDELKQAFYKFHATLMEPWDGPAAVSFTDGNLIGATLDRNGLRPQRYAITEDDRVIMASEAGALALDQSKIIEKGRLTPGKMFVVDMEQGRIISDAEIKQQVCGSKPYGDWINKYQIRLEELPDPRLVFSNLSQESIFRYQQVFGYSREDIDLILKPMAQDGKEPIGSMGTDIPLAILSQKPQHLASYFKQLFAQVTNPPIDPIREKVVMSLAGFMGNNGNILEENAMQCHCVGIKHPILTNLELEKLRSIDTGVFQSKTLQTYFRAD-GKPGALAKGIDRLCRYAVDAVEDGFQVIILSDRALDSEHAAIPSLLAVSAVHHHLIRKGYRGAVGIVVEAGDVWEVHHFATLIGFGATAVNPYLALETIAGFETELQ-EKKEKLFQNYIYAVNNGLLKIFSKMGISTLQSYHGAQIFEILGLHKNVVDNYFTGAVSRIGGLGLDEIAKETLIKHNRIFKLANRPDAILPTGGNYKWKRKGEQHLFNPQTIHLLQNATRKNDYSTYKQYSKLVNEQTNQAYTIRGLFEFNYNRPAVSLEEVEPVESIFKRFATGAMSFGSISHEAHSTLAIAMNRIGGKSNTGEGGEDELRYEVLENGDSMRSAIKQIASARFGVTSYYLTNADELQIKMAQGAKPGEGGQLPGHKVDDWIAKVRHATPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSKAGVGTIAAGVAKAHADVILVSGFDGGTGASPLTSIQHAGLPWELGLAEAHQTLVKNRLRSRVVLQTDGQLKTGRDIAIATLLGAEEWGVATAALVTAGCIMMRKCHLNTCPVGVATQDPNLRKLFTGEADHVVNLFYFLAQELRETMAELGFRSVEEMVGQSDALKVRAIDQEDWKQKDLDLSAILYK-APDNGLSLFKTEDQDHGLSEILDHDLIKAAQPALFNKEPIYKEFEVKNTNRAIGTMLSNEVSKIYKSQGLPADTINFKFKGSAGQSFGAFATKGISLHLEGEANDYVGKGLSGARLSIFPFSTTKYVPEQNIIIGNVALYGATSGELFVRGQAGERFAVRNSGATAVVEGLGDHGCEYMTGGEVLVIGDTGSNFAAGMSGGLAWIYNVKGDFANKCNKEMVDLDPLDEQDELRITHLLKKHLQLTDSSVAKFLLDDWATQSAHFIKVFPKEYKAVLMNKSNKLKIS 1503
BLAST of glutamate synthase vs. nr
Match: gi|1223505222|ref|WP_091542351.1| (glutamate synthase large subunit [Thermoflexibacter ruber]) HSP 1 Score: 1523.45 bits (3943), Expect = 0.000e+0 Identity = 755/1483 (50.91%), Postives = 1008/1483 (67.97%), Query Frame = 0
Query: 1 LVCDLKAIPTRYIVDSALTILENMEHRGAYGAEENTGDGAGILVQIPHLFFKTFALRSEINIPCSRFYGVGMFFFPKDPIKKNFCKSIIRKYIYLYDMVTVITREVPVDNTMIGSMALESEPDMEQWFVKYKDHDQN-GDLENQLFFLR---SSILNDTYQQDAALIDDFYISSFSSKTIVYKGQLSAVQLRGYFLDLQDDRFTSAVAIVHSRFSTNTVPKWKLAQPFRCISHNGEINTIQGNINWWEAREKQLNISETLDSKLRNVLPICDPYISDSGNFDNVAEFLLRSGRSLPHALMMMIPEAWQNDDSLPSYKKSFYEYHDAIMEPWDGPASICFTDGTIVGAALDRNGLRPSRYLITEDDIIVLGSEAGCLSIDPSRIVKKSRLEPGKMLVIDLDEKRIIADEEVKSTICKRYPYQDWIEKQTVYVDDLKSSGPRRKEVDFDNIVRNQLAFGFSREDEDLILQIMAVQGKEPIGSMGADVPLAILSKIAQHPSNYFKQQFAQVTNPPIDSLREKFYMSLKTYLGGSGKIVDVGEDEAAVLRLDSPILTREVFDRLEYLDDSNFSFSRVSLRFK---EEEGIEAALNRINNEVEMIAHDS-NIICLSDIEVDEHYLAVPTLLAIGSVHHFMISR---------VEGGDIWETHHFATLLSYGADAIFPYLAYQSCQVIARKW---HNEDEEFLMASYTKAAEKGLLKIMSKLGVSTINSYRGAQTFEALGVSQSVVSKCFKGTISRVGGMDFVGFQKELNAKHRLAFGD--LRQNVLPDIGLHQWKRKGEFHLFNPQSIHLLQHAVQRNDFSTYKLFAKEIDKVSRNA--------------------------------------------------TMNRIGGKSNSGEGGEDEFRFKSLENGDSMMSAIKQVASGRFGVTINYLTNAKEIQIKMAQGAKPGEGGQLPGHKVDDNIARVRNSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANQEARISVKLVSKMGVGVIASGVAKAHADHILISGADGGTGASPLSSIRHTGLPWELGLSEAHQTLVKNKLRDRVVLQADGQMRTGRDIAIATMLGAEEWGIATAALVVEGCIMMRKCHLNTCPVGIATQDKELRKKFNGKVEHLVNYFHFLAHHLRGIMADVGVSSVNDLVGRTDLLEIANTDNHWKTRDLNLEALLYKFEPGSDQTMYASQKQNHELEHVLDRELIMQTTLARSNNSEVNRVFKIESTNRAVGTMLSNEIAKMYGSKGMQKRSLNIHFRGSAGQSFGAFAAHGLSLVLEGEANDYVAKGLSGAEITILPDRNSKFASHKNIILGNVALYGATSGELMIRGMAGDRFGVRNSGAHAVVEGIGHNGCEYMTGGSVIVLGSIGNNFGAGMSGGYAFIYSSQDEILRNINHEMVSVDTLEVADEVFLRDKIKKFYELTNSNVALNILTDWELLKLNFIKIFPKELKAILKAKDHSV 1411
V ++K + IV+ AL +L MEHRGA G E NTGDGAGIL+Q+PH FF + +++P YGVGM FFPKD + C++ + + I + + R VP DN+MIG A +EP +EQ F+K H + E +LF LR + I+N+T A L FY +S S KTI+YKGQL+ Q+R YF DL D+ SA+A++HSRFSTNT P WKLAQPFR I+HNGEINT++GN++W+ A E ++ + PIC+P SDS N DNV E L+ SGRSLPH +MM+IPEAW ++ + + +K+FYE+H ++MEPWDGPASI FTDG IVGA LDRNGLRPSRY +T +D++++ SE+G L +D S+++KK RL+PG+M ++D+++ RIIADEEVK IC + PY +W+++ + + D+ S + + +D +++ Q++ G++ ED ++ MA+ G E +GSMG D PLA+LS+ +QH SNYFKQ FAQVTNPPID +RE+ MSL +++G S ++ L L P+L+ E +++ Y+D F + + FK +E +E L+RI E D +II LSD D + +P+LLA +VHH ++ + VE D+WETHHFATL+ YGA + PYLA+++ + +K EE L +YTKA K LLKI +K+G+ST+ SY+GAQ FEALG+ + V+ + F GTISR+GG+ KE+ +HRLAF L L G++QWK++GE H+FNPQ+IHLLQ + +RND+ +K ++K ID ++ A MNRIGGKSNSGEGGEDE R+K L NGDSM SAIKQVASGRFGVT YL+NA+E+QIKMAQGAKPGEGGQLPGHKVD+ I R R+STPGVGLISPPPHHDIYSIEDLAQLIFDLKNAN+EARI+VKLVS+ GVG +A+GV KAHAD +LI+G DGGTGASPLSSIRH GLPWELGL+E HQTLVKNKLR R+++QADGQ+RTGRD+AIA +LGAEEWG+ATAALV GC+MMRKCHLNTCPVG+ATQ++ELR F GK E++VN F FLA LR IMAD+G +VN++VG++D LE+ HWK ++L+L A+LYK D +Y ++QNH +++VLD +L+ + A N +V FKI + +RAVGTMLSNEI+K YG++G+ + +++ FRG+AGQSFGAFAA GL+L LEG++NDY KGLSGA + + PDR + F +N I+GNVA YGATSGE IRGMAG+RF VRNSG VVEGIG +GCEYMTGG V+VLG G NF AGMSGG A+I++ + +N EMVS++TL D+ +R ++ + T+S +A IL DWE + F+K+ P + K L+ K+ S+
Sbjct: 28 FVANIKGRKSHQIVEDALKMLTRMEHRGACGCEPNTGDGAGILIQVPHEFFLDECTKLGLDLPPYGEYGVGMVFFPKDEKLREECRATLNRNIKKLKLELIGYRTVPTDNSMIGETAFLAEPHIEQVFIKRPSHITDLMAFERKLFVLRNYATRIINETV---AGLQGAFYFTSLSYKTIIYKGQLTTGQVRPYFPDLHDEHIVSALAVIHSRFSTNTFPSWKLAQPFRYIAHNGEINTVKGNVSWFTAAEVDFFSPYFSKEEMEMLRPICNPSQSDSANLDNVIEMLVLSGRSLPHVMMMLIPEAWDGNEQMDATRKAFYEFHASLMEPWDGPASITFTDGNIVGATLDRNGLRPSRYCVTNEDVVIMASESGALEVDQSKVIKKGRLQPGRMFIVDMEQGRIIADEEVKEQICSQQPYGEWLKQYKIRLSDVPSPAGKFRPLDEKALLKRQISAGYTSEDLKYVIGPMAMTGLEALGSMGLDTPLAVLSEQSQHLSNYFKQLFAQVTNPPIDPIRERMIMSLISFVGQSKNLLTETPQHCHTLELQQPVLSNEDLEKIRYIDHKGFQSKTIYMYFKADGKEGSLERGLDRICQYAEHAVEDGFSIIILSDRSFDSSHAQIPSLLATAAVHHHLLRKGMRGSVGIVVETSDVWETHHFATLIGYGASGVNPYLAFETISYMKQKGLLDTELSEEKLHYNYTKAVGKELLKIFAKMGISTLQSYQGAQIFEALGIHKEVIDRYFTGTISRIGGLKLDDIAKEVLIRHRLAFPTEPLPTQRLEVGGVYQWKQRGEAHIFNPQTIHLLQESTKRNDYQLFKKYSKLIDDQTQKALTLRGMLKFKKRNPIPIEEVEPIENIFKRFATGAMSFGSISWEAHTTLAIAMNRIGGKSNSGEGGEDERRYKKLANGDSMNSAIKQVASGRFGVTSYYLSNAQELQIKMAQGAKPGEGGQLPGHKVDEWIGRTRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEAGVGTVAAGVCKAHADVVLIAGHDGGTGASPLSSIRHAGLPWELGLAETHQTLVKNKLRSRIIVQADGQIRTGRDLAIAALLGAEEWGVATAALVATGCVMMRKCHLNTCPVGVATQNEELRALFTGKPEYVVNLFTFLALELREIMADLGFRTVNEMVGQSDCLEVREDIKHWKHKNLDLSAVLYKAPVTLDVGLYHQEEQNHGIDNVLDWQLLEKAKPALQNKEKVYAQFKIINIDRAVGTMLSNEISKKYGAEGLPQDTIHFKFRGTAGQSFGAFAAKGLTLELEGDSNDYFGKGLSGARMILYPDRTANFEPSENSIVGNVAFYGATSGEAYIRGMAGERFAVRNSGVKTVVEGIGDHGCEYMTGGLVVVLGKTGRNFAAGMSGGVAYIWNKDGDFEAKLNKEMVSLETLSEEDQSIIRMFVENHAQYTDSQLAKYILKDWEKVIKQFVKVMPNDFKKALEKKNISL 1507 The following BLAST results are available for this feature:
BLAST of glutamate synthase vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 1
BLAST of glutamate synthase vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of glutamate synthase vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold3_size1495701:850284..854734- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold3_size1495701-snap-gene-8.0 ID=maker-scaffold3_size1495701-snap-gene-8.0|Name=glutamate synthase|organism=Tigriopus kingsejongensis|type=gene|length=4451bp|location=Sequence derived from alignment at scaffold3_size1495701:850284..854734- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'glutamate synthase' has the following synonyms
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