BLAST of glutamate synthase vs. L. salmonis genes
Match: EMLSAG00000004194 (supercontig:LSalAtl2s:LSalAtl2s221:469662:476711:-1 gene:EMLSAG00000004194 transcript:EMLSAT00000004194 description:"augustus_masked-LSalAtl2s221-processed-gene-4.2")

HSP 1 Score: 301.982 bits (772), Expect = 2.478e-87
Identity = 166/398 (41.71%), Postives = 218/398 (54.77%), Query Frame = 0

Query:    2 PEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTG-RSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKL-------------------------------IMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQ-------------------------------RLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI 336
            P  I  YF DL + +  + L +VH RFSTNT P+WE A P R +  NGEINTLRGNV+ M  RE +M S  F   +  L+PI+ P  SDS  +D VVE L H G RSLPE MM ++PEAW+    M   +K FY + +  MEPWDGPA + FTDG Y+GA+LDRNGLRPSRY +TK+ ++                                 +SE+GV+D+ P  +   GRL+PGK+ LVD +  ++I+D E+KS I   RP+ ++LN  S++LK I         E   +  R+                                +F Y  E ++ ++ PM    KEALGSMG DTPLA LS    LI  YFKQLFAQVTNPP+D  RE+IV  +
Sbjct:  211 PCQIWNYFTDLMDPEFESHLLIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNVNLMEAREGVMVSPDF-EDLSTLYPIVEPDMSDSGCLDNVVEFLVHVGDRSLPEAMMTLVPEAWQNDDQMPSNKKNFYHWASFYMEPWDGPALLTFTDGRYVGAILDRNGLRPSRYYITKNNRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEVGVIDIDPTQIIKKGRLQPGKILLVDTDVQKVIDDHELKSSIAXSRPHGDFLNK-SIDLKIIKEAGSEYAAEISSLPRRRPINLHENDFNNNFDYGEENILEKLWRGDRRLPIFGYNIETLEMLMLPMFKTKKEALGSMGNDTPLACLSEMQPLIYEYFKQLFAQVTNPPIDPFREKIVMSL 606          

HSP 2 Score: 133.65 bits (335), Expect = 3.709e-32
Identity = 73/139 (52.52%), Postives = 95/139 (68.35%), Query Frame = 0

Query:  533 QAVRLSDQESYNVYAKAINEQSENL--MTIRGLFEFD--NFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            + V  +  E+ N Y K ++   E +   +IRG FE D     P+ + EVE  + IVKRF TGAMS+GSIS EAH  LA+AMN++GGKSN+GEGGE+  R+  D+    R SAIKQVASGRFGV++ YL +A E+QIKMA
Sbjct:  765 EGVEATKYENKNAYQKYVDATMETIKKCSIRGQFEIDFSKGTPVDVSEVELASNIVKRFVTGAMSFGSISAEAHGTLAVAMNKLGGKSNTGEGGENEERYLNDI----RRSAIKQVASGRFGVSASYLAHADELQIKMA 899          

HSP 3 Score: 133.65 bits (335), Expect = 3.975e-32
Identity = 58/84 (69.05%), Postives = 71/84 (84.52%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SR+V++ DGQL+TG DV +A +LGA+EFGF+TAPL+  GC MMRKCHLNTCPVGIATQD  LRK F+G PEHV+N+F+ IAE
Sbjct:  995 LRSRVVLQTDGQLRTGFDVMVAGILGADEFGFSTAPLIVLGCTMMRKCHLNTCPVGIATQDPILRKKFEGQPEHVVNYFFLIAE 1078          

HSP 4 Score: 73.559 bits (179), Expect = 1.725e-13
Identity = 39/86 (45.35%), Postives = 50/86 (58.14%), Query Frame = 0

Query:  326 DGIREEIVTDINRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEG 411
            +G++  I++D N GVN+   P+ A LA   VHH +   R RS   I +ES E RE HH   L G+GA A+ PYMV EI  M   EG
Sbjct:  683 EGVQLLILSDRNVGVNR--IPVSAALASGAVHHHLIATRLRSKCAIIVESGEAREVHHLCVLIGFGADAICPYMVLEIAAMLKDEG 766          

BLAST of glutamate synthase vs. SwissProt
Match: gi|12644435|sp|Q43155.3|GLTB_SPIOL (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Short=SoFdGOGAT)

HSP 1 Score: 414.075 bits (1063), Expect = 6.055e-126
Identity = 273/785 (34.78%), Postives = 395/785 (50.32%), Query Frame = 0

Query:    3 EDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMS---PERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI-----NRG---------VNQEFAPIP--------ALLACSFVHHQM-------------------NRL--------RKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYR--------------------------------------------------GSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEF-DNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQP--DV---------------NGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            E +G ++ DLQ     +  A+ H+R+STNT P W LAQP RF+  NGEINT++GN++ MR RE  ++S V+  +  ++ P   P  SDSA++D   ELL  +GR+  E +M+++PEA++ H T+    PE   FY+Y    ME WDGPA + F+DG  +GA LDRNGL P+RY  T    + ++SE+GV+ +    V   GRL PG M  VD++ G++  + E+K ++ S  PY +W+     +LK + + ++   +E   I   Q+ F Y+ ED+Q VI  MA  GKE    MG D PLAV+S KP ++ +YFKQ FAQVTNP +D +RE +V  +      RG          +Q   P P        AL+    +  QM                   NRL        R  S   +  + +E  EP   A        AV+ +++   +RM      +    +  +   F   IG G   +   + + T R +R                                                  G+QIFEI G     V+  F  + S++ G+ L E+ RE    +  A+  +   +   L   G  ++R  GE H+ NP     L +AVR   + +Y VY + +  +  +++  R L EF  +  PI + +VEP T IV+RF TG MS G+ISRE HE +AIAMNR+GGKSNSGEGGED  R++P  DV               NGD+  SAIKQVASGRFGVT  +L NA +I+IK+A
Sbjct:  187 EVLGMFYYDLQNERYTSPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMRSREPSIQSPVWRGRENEIRPYGNPKASDSANLDSAAELLIRSGRTPEEALMILVPEAYKNHPTLMIKYPEAVDFYDYYKGQMETWDGPALLLFSDGKTVGACLDRNGLAPARYWRTVDNVVYVASEVGVLPMDESKVTMKGRLGPGMMISVDLSSGQVYENTEVKKRVASSNPYGKWVKENLRSLKAVNFLSRAL-LENDTILRNQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVMSQKPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENASQVILPSPVLNEGELEALVNDPLLKAQMLPIFFDIRKGVEGTLEKRLNRLCEAADEAVRNGSQMLVLSDRSEELEPTRPAIPILLAVGAVHQHLIQNGLRMYTS--IVVDTAQCFSTHQFACLIGYGASAICPYLALETCRQWRLSNKTVNLMRTGKIPTVTIEQAQNNFCKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGKDVVDIAFQGSVSKMGGLTLDELARETLSFWVKAFSEDTAKR---LENFGFIQFRPGGEYHVNNPEMSKLLHKAVRNKSESAYAVYQQHLANRPVSVL--RDLLEFKSDRAPISVGKVEPATSIVERFCTGGMSLGAISRETHEAIAIAMNRLGGKSNSGEGGEDPIRWRPLTDVVDGYSSTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIA 963          

HSP 2 Score: 103.219 bits (256), Expect = 3.709e-21
Identity = 41/84 (48.81%), Postives = 59/84 (70.24%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R+++  DG LK G DV +A  +GA+E+GF +  ++A+GC+M R CH N CPVG+A+Q +ELR  F G P  ++NFF Y+AE
Sbjct: 1091 LRERVILRVDGGLKCGVDVMMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNFFLYVAE 1174          

BLAST of glutamate synthase vs. SwissProt
Match: gi|121446|sp|P23225.1|GLTB_MAIZE (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Flags: Precursor)

HSP 1 Score: 404.445 bits (1038), Expect = 4.733e-122
Identity = 275/785 (35.03%), Postives = 388/785 (49.43%), Query Frame = 0

Query:    3 EDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMS---PERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI-----NRG----VNQEFAPIPALLACSFVHHQMNRLRKRS---------YFDI-------------------------------FIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGS---------------------------------QIFEIVGFN-------SQFVEKY----------FPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEF-DNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQP--DV---------------NGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            E +G ++ DLQ     +  A+ H+RFSTNT P W LAQP R +  NGEINT++GN++ MR RE  +KS V+  +  ++ P   P  SDSA++D   ELL  +GRS  E +M+++PEA++ H T+S   PE   FY+Y    ME WDGPA + F+DG  +GA LDRNGLRP+RY  T    + ++SE+GV+ +    V   GRL PG M  VD+  G+++ + E+K  + S  PY  WL   +  +K + + + T  ++   +   Q+ F Y+ ED+Q VI  MA  GKE    MG D PLAVLS +P L+ +YFKQ FAQVTNP +D +RE +V  +      RG    V  E A   AL +      ++  L   S         YFDI                                 + +E  EP   A        A++ +++   +RM      +    +  +  +F   IG G   V   + + T R +R S                                 +I   +G +       +Q  E Y          F  + S+I G+ L E+ RE    +  A+  +   +   L   G  + R  GE H  NP     L +A+R     +Y VY + +  +  N++  R L E   +  PIP+ +VE  T IV+RF TG MS G+ISRE HE +AIAMNRIGGKSNSGEGGED  R+ P  DV               NGD+  SAIKQVASGRFGVT  +L NA +I+IK+A
Sbjct:  284 EVLGQFYLDLQNELYKSPFAIYHRRFSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMRSRETTLKSPVWRGREHEICPFGDPKASDSANLDSTAELLLRSGRSPAEALMILVPEAYKNHPTLSIKYPEVTDFYDYYKGQMEAWDGPALLLFSDGRTVGATLDRNGLRPARYWRTSDDFVYVASEVGVIPMDESKVVMKGRLGPGMMITVDLQTGQVLENTEVKKTVASASPYGTWLQECTRLIKPVNFLSSTI-MDNETVLRHQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVLSQRPHLLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENADQVALSSPVLNEGELETLLNDSKLKPKVLSTYFDIRKGLDGSLDKTIQALCEEADAAVRSGSQLLVLSDRSEAPEPTRPAIPILLAVGAIHQHLIQNGLRMSAS--IVADTAQCFSTHHFACLIGYGASAVCPYLALETCRQWRLSNKTLNLMRNGKMPTVTIEQAQRNFIKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGQEVVDLAFCGSVSKIGGLTLDELGRETLSFWVKAFSEDTAKR---LENFGFIQSRPGGEYHANNPEMSKLLHKAIREKRDNAYTVYQQHLASRPVNVL--RDLLELKSDRAPIPIGKVESATSIVERFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWNPLTDVVDGYSPTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIA 1060          

HSP 2 Score: 101.679 bits (252), Expect = 1.026e-20
Identity = 39/84 (46.43%), Postives = 60/84 (71.43%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R+V+  DG  ++G+DV IA  +GA+E+GF +  ++A+GC+M R CH N CPVG+A+Q +ELR  F G P  ++N+F ++AE
Sbjct: 1188 LRERVVLRVDGGFRSGQDVLIAAAMGADEYGFGSVAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLFVAE 1271          

BLAST of glutamate synthase vs. SwissProt
Match: gi|1707984|sp|P55037.1|GLTB_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 1; AltName: Full=Fd-GOGAT)

HSP 1 Score: 395.971 bits (1016), Expect = 3.694e-119
Identity = 182/339 (53.69%), Postives = 244/339 (71.98%), Query Frame = 0

Query:    5 IGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDINRGVNQE 343
            +G Y+ +L + D+ + LALVH RFSTNT P+WE + P+R++  NGEINT+RGNV+ M+ R+ + +S +FG  + K+ P+I    SDS   D  +ELL   GRSLP  +MMMIPE W  H++MS E+KAFY+Y++C+MEPWDGPAS+ FT+G  +GA+LDRNGLRPSRY VTK   +IM+SE GV+ + PE V   GRL+PG+MFLVDM +GRII DEEIK +IVS+ PY EWL     +L+ +P        +   ++ RQ  F YTFE+++ ++ PM   G EA+GSMG DTPLAVLS+KP+L+ NYF+QLFAQVTNPP+D IREEI+T     +  E
Sbjct:  232 VGQYYPELHDPDMESALALVHSRFSTNTFPSWERSHPYRYIAHNGEINTMRGNVNWMQARQALFESSLFGEDMAKVQPVINIDGSDSTIFDNALELLYLAGRSLPHAVMMMIPEPWSAHESMSQEKKAFYKYHSCLMEPWDGPASIAFTNGKMMGAVLDRNGLRPSRYYVTKDDLVIMASEAGVLPIEPERVAKKGRLQPGRMFLVDMEQGRIIADEEIKQEIVSQHPYGEWLAANLKSLEQLPSPGNVPGTDAESLRQRQMAFGYTFEELRILLAPMGRDGVEAIGSMGADTPLAVLSDKPKLLYNYFQQLFAQVTNPPIDSIREEIITSAETTIGGE 570          

HSP 2 Score: 364.77 bits (935), Expect = 5.648e-108
Identity = 188/360 (52.22%), Postives = 246/360 (68.33%), Query Frame = 0

Query:  309 ISNYFKQLFAQVTNPPLDGIREEIVTDINRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQ-PDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +      LF +       G    I++D  R V+ E A IPALLA S +HH + R   R+   + +ES EPRE HHFA L GYG  A+NPY+  E +   + EG +  +D + A  N+ KA  KG++KV +KIGIST++SYRG+QIFE VG N   +++YF  T+SRI+G  L  I +E   R+++A+     D    L++GG+Y+WR++GE HLF+P T+  LQ+AVR  + E Y  YA  +NEQ++   T+RGL +F + + IPLEEVEP   I+KRFKTGAMSYGSIS+EAHE+LAIAMNRIGGKSN+GEGGED  RF   +  GDS+NSAIKQVASGRFGVTS YL+ AKEIQIKMA
Sbjct:  625 LKTALDNLFTEADQAISQGANLIILSD--RQVSAEKAAIPALLAVSGLHHHLIRNGSRTKVGLVLESGEPREVHHFAVLLGYGCGAINPYLAFETLDGMIAEGLLVNVDHKTACKNYIKAATKGVIKVASKIGISTIQSYRGAQIFEAVGLNQSVIDEYFCRTSSRIQGSDLGVIAQEAILRHQHAFAPRPGDLHT-LDVGGEYQWRKDGEEHLFSPQTIHLLQRAVREGNYELYKQYAALVNEQNQKFFTLRGLLDFQDRESIPLEEVEPIEAIMKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEGGEDPERFTWTNDQGDSKNSAIKQVASGRFGVTSLYLSQAKEIQIKMA 981          

HSP 3 Score: 139.043 bits (349), Expect = 2.245e-32
Identity = 63/83 (75.90%), Postives = 69/83 (83.13%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIA 778
            L SRIVVE DGQ+KTGRDVAIA LLGAEEFGF+TAPLV+ GCIMMR CHLNTCPVGIATQ+ ELR  F G P H +NF  +IA
Sbjct: 1109 LRSRIVVETDGQMKTGRDVAIAALLGAEEFGFSTAPLVSLGCIMMRACHLNTCPVGIATQNPELRAKFTGDPAHAVNFMTFIA 1191          

BLAST of glutamate synthase vs. SwissProt
Match: gi|81556736|sp|P96218.3|GLTB_MYCTU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain)

HSP 1 Score: 392.889 bits (1008), Expect = 3.228e-118
Identity = 181/332 (54.52%), Postives = 243/332 (73.19%), Query Frame = 0

Query:    8 YFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFG--PQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDIN 337
            ++ DLQ+  L + L +VH RFSTNT P+W LA PFR +  NGEINT+ GN + MR RE ++K+D+FG    +EKLFPI  PG SD+A  D V+ELL   GRSL   ++MMIPEAWE+H++M P R+AFY+Y+A +MEPWDGPAS+ FTDG  +GA+LDRNGLRPSR  VT  G ++M+SE GV+D+ P  V    RL+PG+MFLVD  +GRI++DEEIK+ + +E PYQEWL+N  + L ++P   +   +    I  RQ  F YT+E++  ++ PMA +G E +GSMGTDTP+AVLS +P+++ +YF QLFAQVTNPPLD IREE+VT + 
Sbjct:  209 FYLDLQDERLTSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALIKTDIFGSAADVEKLFPICTPGASDTARFDEVLELLHLGGRSLAHAVLMMIPEAWERHESMDPARRAFYQYHASLMEPWDGPASMTFTDGTVVGAVLDRNGLRPSRIWVTDDGLVVMASEAGVLDLHPSTVVRRMRLQPGRMFLVDTAQGRIVSDEEIKADLAAEHPYQEWLDNGLVPLDELP-EGKDVRMPHHRIVMRQLAFGYTYEELNLLVAPMARLGAEPIGSMGTDTPVAVLSQRPRMLYDYFHQLFAQVTNPPLDAIREEVVTSLQ 539          

HSP 2 Score: 351.288 bits (900), Expect = 2.720e-103
Identity = 174/336 (51.79%), Postives = 231/336 (68.75%), Query Frame = 0

Query:  337 NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAY---PNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNF--DPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +R  ++E APIP+LLA + VHH + R R R+   + +ES + RE HH A L G+GA+A+NPY+V E I   +  G I G+D   A++N+ KA GKG+LKVM+K+GISTL SY G+Q+F+ VG + Q +++YF        GI L +I  ++  R++ AY   P+    + L   +GG+Y+WRR GE HLFNP TV KLQ + R    + +  Y + +++QSE + ++RGL +F      P+PL+EVEP +EIVKRF TGAMSYGSIS EAHE LAIAMNR+G +SN GEGGED  RF  D NGD R SAIKQVAS RFGVTSHYLTN  ++QIKMA
Sbjct:  634 DRESDEEMAPIPSLLAVAGVHHHLVRERTRTQVGLVVESGDAREVHHMAALVGFGAAAINPYLVFESIEDMLDRGVIEGIDRTAALNNYIKAAGKGVLKVMSKMGISTLASYTGAQLFQAVGISEQVLDEYFTGLTCPTGGITLDDIAADVAARHRLAYLDRPDERAHREL--EVGGEYQWRREGEYHLFNPETVFKLQHSTRTGQYKIFKEYTRLVDDQSERMASLRGLLKFRTGVRPPVPLDEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGARSNCGEGGEDVKRFDRDPNGDWRRSAIKQVASARFGVTSHYLTNCTDLQIKMA 967          

HSP 3 Score: 135.576 bits (340), Expect = 2.835e-31
Identity = 63/84 (75.00%), Postives = 70/84 (83.33%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  RIVV+ DGQLKTGRDV IATLLGAEEFGFATAPLV +GCIMMR CHL+TCPVG+ATQ+  LR+ F G PE V NFF +IAE
Sbjct: 1095 LRDRIVVQVDGQLKTGRDVMIATLLGAEEFGFATAPLVVAGCIMMRVCHLDTCPVGVATQNPLLRERFTGKPEFVENFFMFIAE 1178          

BLAST of glutamate synthase vs. SwissProt
Match: gi|239938894|sp|P39812.3|GLTA_BACSU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=NADPH-GOGAT)

HSP 1 Score: 371.318 bits (952), Expect = 2.109e-110
Identity = 172/333 (51.65%), Postives = 231/333 (69.37%), Query Frame = 0

Query:    2 PEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVT 334
            PE +  ++ DLQ+   V+  ALVH RFSTNT PTWE A P R++  NGEINTLRGN++ MR RE+   S+ FG  + K+ PI+    SDS+ +D   E     GR      MM+IPE W ++  MS E++AFYEY++ +MEPWDGP ++ FTDG  IGA+LDRNGLRP+RY VTK   +I SSE+GV++V  E+V    RLEPGKM L+D+ +GRII+DEE+K++I +E PYQ+WL    + +   P + +       D+ TRQ+ F YT+EDIQ+ + P+   GK+ LGSMG D PLAVLS++ Q + NYFKQLFAQVTNPP+D IRE++VT
Sbjct:  204 PEQVDAFYSDLQDEAFVSAFALVHSRFSTNTFPTWERAHPNRYLVHNGEINTLRGNINWMRAREQQFVSESFGEDLNKILPILNADGSDSSILDNAFEFFVMAGRKPAHTAMMLIPEPWTENTHMSKEKRAFYEYHSSLMEPWDGPTAISFTDGKQIGAILDRNGLRPARYYVTKDDYIIFSSEVGVIEVEQENVLYKNRLEPGKMLLIDLEEGRIISDEEVKTQIATEYPYQKWLEEELVQVNPDPESREEEQFS--DLLTRQKAFGYTYEDIQKYLIPVIKEGKDPLGSMGNDAPLAVLSDRAQSLFNYFKQLFAQVTNPPIDAIREQLVT 534          

HSP 2 Score: 339.732 bits (870), Expect = 3.042e-99
Identity = 178/356 (50.00%), Postives = 232/356 (65.17%), Query Frame = 0

Query:  313 FKQLFAQVTNPPLDGIREEIVTDINRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFD-NFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
             K +F Q       G+   I++D  + +N+   PIP LLA S +H  + R   R+   I +ES E RE HHFA L GYGA A+NPY+     + ++ EG +  +  ++AV  + K+I +G++KVM+K+GIST++SYRG+QIFE VG +   +++YF  TAS++ GI L  I  E   R++ AY +   D    L  G D++WR  GE H FNP T+  LQ A R +D   +  Y KA +E  E +  +R LF FD N  P+ LEEVE    IVKRFKTGAMS+GS+S+EAHE LAIAMNR+GGKSNSGEGGED  RF PD NGD R SAIKQ+ASGRFGV SHYL NA E+QIKMA
Sbjct:  597 LKDMFTQAEKAISQGVSLLILSD--KKMNERLTPIPPLLAVSALHQHLIRKGLRTKVSIIVESGEAREVHHFAALIGYGADAINPYLAYATYKQEIDEGRLD-ISYEEAVSKYGKSITEGVVKVMSKMGISTVQSYRGAQIFEAVGISRDVIDRYFSGTASQLGGIDLQTIAEEAQRRHREAYQD---DYSKTLEPGSDFQWRNGGEHHAFNPKTIHTLQWACRRNDYNLFKQYTKAADE--ERIGFLRNLFAFDGNRKPLKLEEVESAESIVKRFKTGAMSFGSLSKEAHEALAIAMNRLGGKSNSGEGGEDPKRFVPDENGDDRRSAIKQIASGRFGVKSHYLVNADELQIKMA 944          

HSP 3 Score: 132.88 bits (333), Expect = 2.054e-30
Identity = 56/84 (66.67%), Postives = 69/84 (82.14%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R+V+E DG+L TGRDV +A LLGAEEFGFATAPLV  GC+MMR CHL+TCPVG+ATQ+ ELRK F G P+H++N+  +IAE
Sbjct: 1072 LRDRVVLETDGKLMTGRDVVMAALLGAEEFGFATAPLVVLGCVMMRACHLDTCPVGVATQNPELRKKFMGDPDHIVNYMLFIAE 1155          

BLAST of glutamate synthase vs. SwissProt
Match: gi|417073|sp|Q03460.1|GLSN_MEDSA (RecName: Full=Glutamate synthase [NADH], amyloplastic; AltName: Full=NADH-GOGAT; Flags: Precursor)

HSP 1 Score: 362.459 bits (929), Expect = 1.946e-106
Identity = 179/367 (48.77%), Postives = 236/367 (64.31%), Query Frame = 0

Query:    2 PEDIGPYF-KDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFG---PQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDI------------------PYTNQTCPIETIDIK---TRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDINRGVNQE 343
            P  +G Y+  DL      + +AL+H RFSTNT P+W+ AQPFR +  NGEINTLRGNV+ ++ RE ++K    G     ++K  PI+    SDS   D V+E L H+G+SLPE +MMMIPEAW+  + M P+RKAFYEY + +MEPWDGPA + FTDG Y+GA LDRNGLRP R+ VT SG++IM+SE+GVVD+ PEDV   GRL PG M LVD  K  ++ND+ +K +    RPY +WL    + LKDI                  P +N    +E + I+      + F Y+ E ++ ++ PMA  G EALGSMG DTPLAV+SN+ +L   YFKQ+FAQVTNPP+D IRE+IVT +   V  E
Sbjct:  288 PAQLGEYYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREGLLKCKELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLLHSGKSLPEAVMMMIPEAWQNDKNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIPPEDVCRKGRLNPGMMLLVDFEKQIVVNDDALKEQYSLARPYGDWLEKQKIELKDIIDSVHESDIVPPTISGVPPLSNDDVDMENMGIQGLLAPLKAFGYSVESLEILLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMRCMVGPE 654          

HSP 2 Score: 290.426 bits (742), Expect = 5.867e-82
Identity = 162/347 (46.69%), Postives = 223/347 (64.27%), Query Frame = 0

Query:  337 NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEII-RMQV-------KEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLID----KRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEF-DNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRN---SAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +R  +++   + +LLA   VH  + +  +R+   + +ESAEPRE HHF TL G+GA A+ PY+  E I R+QV         G     DE   V  + KA   G++KV+ K+GISTL SY+G+QIFE +G +S+ +EK F  T SR+EG     + ++    ++ A+P+ +      + + L   GDY WR+ GE HL +P  +AKLQ+A R +  ++Y  Y+K I+E ++    +RGL +F D    +P+ EVEP +EIVKRF TGAMSYGSIS EAH  LA AMN IGGKSN+GEGGE  +R +P  +G SRN   SAIKQVASGRFGV+S+YLTNA E+QIKMA
Sbjct:  735 DRAFSKKHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDE--LVKKYFKASTYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELAFPSRIFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTIHELNKAC-NLRGLLKFKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMNTIGGKSNTGEGGEQPSRMEPLADG-SRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMA 1077          

HSP 3 Score: 137.117 bits (344), Expect = 1.250e-31
Identity = 61/84 (72.62%), Postives = 69/84 (82.14%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R  ++ DGQLKTGRDVAIA LLGAEE+GF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEHVINFF+ +AE
Sbjct: 1205 LRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAE 1288          

BLAST of glutamate synthase vs. SwissProt
Match: gi|300680979|sp|Q0DG35.2|GLT2_ORYSJ (RecName: Full=Glutamate synthase 2 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 2; Short=NADH-GOGAT 2; Flags: Precursor)

HSP 1 Score: 356.295 bits (913), Expect = 2.412e-104
Identity = 177/353 (50.14%), Postives = 230/353 (65.16%), Query Frame = 0

Query:    6 GPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFG---PQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDI-----------PYTNQTCPIET--------IDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI 336
            G +F DL +    + +AL+H RFSTNT P+W+ AQP R +  NGEINTLRGN + M+ RE ++K +  G    ++ KL PI+    SDS ++D V+ELL  +GRS PE +MMMIPEAW+    M PERKA YE+ + +MEPWDGPA + FTDG Y+GA LDRNGLRP R+ VT SG++IM+SE+GVVDV P+DV   GRL PG M LVD     ++ND+E+K +    RPY EWL    + L DI           P  +   PI            I T  + F YT E ++ ++ PMA  G+EALGSMG DTPLAV+SN+ +L   YFKQ+FAQVTNPP+D IRE+IVT +
Sbjct:  301 GYFFADLGDESFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNKNWMKAREGLLKCEGLGLTRDEMLKLLPIVDATSSDSGAIDNVLELLIQSGRSAPEAVMMMIPEAWQNDVNMDPERKALYEFFSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTYSGRVIMASEVGVVDVPPQDVSRKGRLNPGMMLLVDFENHCVVNDDELKKEYSKVRPYGEWLKRQRIQLTDIIESVNEAERIAPSISGALPITKENKADMGICGILTPLKAFGYTREALEMLMLPMAKDGQEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSM 653          

HSP 2 Score: 278.485 bits (711), Expect = 5.957e-78
Identity = 163/370 (44.05%), Postives = 232/370 (62.70%), Query Frame = 0

Query:  313 FKQLFAQVTNPPLDGIRE--EIVTDINRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEII-RMQVKEGFITGMD-----EQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLI----DKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNF-DPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRF--QPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
             KQ   ++     + I E   I+   +RG + E   + +LLA   VH  +    +R+   + +ESAEPRE HHF+TL G+GA A+ PY+  E I R+Q+ +G I   D     +++ ++ +  A   G++KV+ K+GISTL SY+G+QIFE +G  S+ V K F  T SR+EG     + ++    ++ A+P+  +         L   GDY WR+NGE HL +P ++AKLQ+A R++ +E+Y  Y++ I E ++   T+RG+ +F    + I L+EVEP  EIVKRF TGAMSYGSIS EAH +LA AMN +GGKSN+GEGGE   R    PD + + R SAIKQVASGRFGV+ +YLTNA E+QIKMA
Sbjct:  715 LKQTLDKICAQAREAIHEGYTILVLSDRGFSSERVAVSSLLAVGAVHQHLVSHLERTRIGLLVESAEPREVHHFSTLIGFGADAICPYLAIEAIWRLQI-DGRIPPNDGKPYTQEQLIEKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLASEVVSKCFEGTPSRVEGATFEMLAQDALRLHEIAFPSRTLPPGSADANALPNPGDYHWRKNGEVHLNDPFSIAKLQEAARINSREAYKEYSRRIYELNKAC-TLRGMLKFREIPNQISLDEVEPAKEIVKRFCTGAMSYGSISLEAHTSLAEAMNTLGGKSNTGEGGEQPCRMVPLPDGSKNPRISAIKQVASGRFGVSIYYLTNAVEVQIKMA 1082          

HSP 3 Score: 134.806 bits (338), Expect = 5.520e-31
Identity = 59/84 (70.24%), Postives = 68/84 (80.95%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R V++ DGQ+KTGRDVA+A LLGAEEFGF+TAPL+  GCIMMRKCH NTCP GIATQD  LR  F G PEHVIN+F+ +AE
Sbjct: 1210 LRGRAVLQTDGQMKTGRDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPAGIATQDPVLRAKFAGKPEHVINYFFMLAE 1293          

BLAST of glutamate synthase vs. SwissProt
Match: gi|300680981|sp|Q9LV03.2|GLUT1_ARATH (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor)

HSP 1 Score: 351.673 bits (901), Expect = 9.182e-103
Identity = 177/353 (50.14%), Postives = 232/353 (65.72%), Query Frame = 0

Query:    8 YFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSD---VFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDI------------------PYTNQTCPIETIDIK---TRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI 336
            Y+ DL      + +ALVH RFSTNT P+W+ AQP R +  NGEINTLRGNV+ MR RE ++K +   +   +++KL PI+    SDS + D V+ELL   GRSLPE +MMMIPEAW+  + + P RK FYEY + +MEPWDGPA + FTDG Y+GA LDRNGLRP R+ +T SG++IM+SE+GVVDV PEDV   GRL PG M LVD  K  +++D+ +K +    RPY EWL    + LKDI                  P +N    +E++ I    +  + F YT E ++ ++ PMA  G EALGSMG DTPLAV+SN+ +L   YFKQ+FAQVTNPP+D IRE+IVT +
Sbjct:  313 YYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSM 665          

HSP 2 Score: 287.73 bits (735), Expect = 5.110e-81
Identity = 160/346 (46.24%), Postives = 226/346 (65.32%), Query Frame = 0

Query:  337 NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYM-VNEIIRMQVKEGFITG------MDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLID----KRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFD-PIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRN---SAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +R  +     + +L+A   VHH + +   R+   + +ESAEPRE HHF TL G+GA A+ PY+ V  + R+QV +G I          +++ V  + KA   G++KV+ K+GISTL SY+G+QIFE +G +S+ ++K F  T SR+EG     + R+  + ++ A+P         +   L   G+Y WR+NGE HL +P  +AKLQ+A R +   +Y  Y+K INE ++    +RGL +F + D  IPL+EVEP +EIVKRF TGAMSYGSIS EAH  LA+AMN++GGKSN+GEGGE  +R +P  +G SRN   S+IKQ+ASGRFGV+S+YLTNA E+QIKMA
Sbjct:  753 DRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQV-DGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQ-SNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADG-SRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMA 1095          

HSP 3 Score: 139.043 bits (349), Expect = 2.755e-32
Identity = 62/84 (73.81%), Postives = 70/84 (83.33%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R V++ DGQLKTGRDVA+A LLGAEEFGF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEHVINFF+ +AE
Sbjct: 1223 LRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAE 1306          

BLAST of glutamate synthase vs. SwissProt
Match: gi|122222486|sp|Q0JKD0.1|GLT1_ORYSJ (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor)

HSP 1 Score: 347.436 bits (890), Expect = 2.611e-101
Identity = 173/353 (49.01%), Postives = 225/353 (63.74%), Query Frame = 0

Query:    6 GPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFG---PQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDI----PYTNQTCP---------------IETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI 336
            G Y+ DL   +  + +ALVH RFSTNT P+W+ AQP R +  NGEINTL+GN + M+ RE +++ +  G    Q  K+ PI+    SDS + D V+ELL   GRSLPE +MMMIPEAW+    M PE+KA YE+ + +MEPWDGPA + FTDG Y+GA LDRNGLRP R+ VT SG+++M SE+GVVDV  +DV   GRL PG M LVD     +++DE +K++     PY EWL    + LKDI    P T +  P                    I T  + F YT E ++ ++ PMA  G EALGSMG DTPLAV+SN+ +L   YFKQ+FAQVTNPP+D IRE+IVT +
Sbjct:  294 GYYYADLGHENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKLGLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGGRSLPEAVMMMIPEAWQNDVNMEPEKKALYEFLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVVMGSEVGVVDVPSKDVLRKGRLNPGMMLLVDFENHTVVDDEALKAQYSKAHPYGEWLKRQKIYLKDIVESVPETERVAPGISGSLTQKNEKKEHAGVNGIVTPLKAFGYTVEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSM 646          

HSP 2 Score: 284.648 bits (727), Expect = 4.782e-80
Identity = 158/345 (45.80%), Postives = 221/345 (64.06%), Query Frame = 0

Query:  337 NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFI------TGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNL-----IDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEF-DNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNG--DSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +RG + +   + +LLA   VH  +    +R+   + +ESAEPRE HHF TL G+GA AV PY+  E I     +G I          +++ V  +  A   G++KV+ K+GISTL SY+G+QIFE +G +S+ + K F  T SRIEG     + R+    ++ A+P+        D +  L   GDY WR+NGE HL +P  +AKLQ+A R++ + +Y  Y++ I E ++    +RG+ +F D  D I ++EVEP +EIVKRF TGAMSYGSIS EAH  LA+AMN++GGKSN+GEGGE  +R +P  NG  + + SAIKQVASGRFGV+S+YLTNA E+QIKMA
Sbjct:  734 DRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVHHFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFPSRAPPPGSADAK-ALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQELNKTC-NLRGMLKFKDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKLGGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMA 1076          

HSP 3 Score: 137.117 bits (344), Expect = 1.046e-31
Identity = 60/84 (71.43%), Postives = 70/84 (83.33%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R +++ DGQLKTG+DVA+A LLGAEEFGF+TAPL+  GCIMMRKCH NTCPVGIATQD  LR+ F G PEHVINFF+ +AE
Sbjct: 1204 LRGRAILQTDGQLKTGKDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAE 1287          

BLAST of glutamate synthase vs. SwissProt
Match: gi|46395959|sp|Q9C102.1|GLT1_SCHPO (RecName: Full=Putative glutamate synthase [NADPH]; AltName: Full=NADPH-GOGAT)

HSP 1 Score: 335.495 bits (859), Expect = 3.202e-97
Identity = 164/345 (47.54%), Postives = 221/345 (64.06%), Query Frame = 0

Query:    2 PEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGR-SLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDI-------PYTNQTCPIETIDIKTRQRL--FNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKPQLISNYFKQLFAQVTNPPLDGIREEIVTDI 336
            P  +  YF DL   + V+  ALVH RFSTNT P+W+ AQP R    NGEINTLRGN + M  RE +MKS  FG +   L PII  G SDSA+ D V+ELL  +G  SLPE +M++IPEAW+  + +S E+ AFYE+ AC MEPWDGPA   F DG Y GA LDRNGLRP R+ +T    +I +SE+G V + P+ +   GRL PG+M LVD  +GRI++D+E+K  I S   ++ WL+   +++  I          + T  ++ + +   + +  F YT E I  ++ PMA  GKE LGSMG D  +A LS++P+L+ +YF+QLFAQVTNPP+D IRE IV  +
Sbjct:  257 PVQVYNYFLDLNNAEYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMKSSRFGEEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKAAFYEWAACQMEPWDGPALFTFADGRYCGANLDRNGLRPCRFYLTSDDMMICASEVGTVGIEPDRIVQKGRLYPGRMLLVDTKEGRIVDDKELKHNIASRYDFRSWLDQELIDMNSIVDSLIESTSVDLTPIVDDVPLADDKTMLAFGYTLEQINMIMAPMANGGKETLGSMGNDAAIACLSDQPRLLYDYFRQLFAQVTNPPIDPIREAIVMSL 601          

HSP 2 Score: 315.849 bits (808), Expect = 1.619e-90
Identity = 164/345 (47.54%), Postives = 231/345 (66.96%), Query Frame = 0

Query:  326 DGIREEIVTDINRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFIT-GMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGL--FEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +G +  +++D  R VN E  P+ ++ AC  VHH + + + RS   +  ES + RE HH  TL GYGA AV PY+  E +   V++  +  G+ E+ A+ NF  AI  GILKVM+K+GISTL+SY+G+QIFE +G +++ + K F  TASRI G+    I  +    ++  YP +   + L +   GD+ +R  GE+H+ +P  +A LQ AVR  ++ +Y  +++   EQ+    T+RG+  F+FD+   IP+E+VEPWTEIV+RF TGAMSYGSIS E+H +LAIAMNR+GGKSN+GEGGED  R Q   NGD+  SAIKQ+ASGRFGVTS YL++A E+QIKMA
Sbjct:  682 EGYKAIVLSD--RNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDAREVHHMCTLLGYGADAVCPYLAMEALTKLVRQNAMKPGITEETAIKNFKHAINGGILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHERGYPTDQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSRTHYEQTRRC-TLRGMLDFDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGGKSNTGEGGEDPARSQRLANGDTMRSAIKQIASGRFGVTSWYLSDADELQIKMA 1023          

HSP 3 Score: 150.214 bits (378), Expect = 7.314e-36
Identity = 65/84 (77.38%), Postives = 75/84 (89.29%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L  R+V++ DGQ++TGRDVAIA LLGAEE+GFAT PL+A GCIMMRKCHLNTCPVGIATQD ELRK F+G PEHV+NFFYY+AE
Sbjct: 1151 LRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALGCIMMRKCHLNTCPVGIATQDPELRKKFEGQPEHVVNFFYYVAE 1234          

BLAST of glutamate synthase vs. nr
Match: gi|654414197|ref|WP_027885635.1| (glutamate synthase large subunit [Mesonia mobilis])

HSP 1 Score: 897.886 bits (2319), Expect = 0.000e+0
Identity = 450/754 (59.68%), Postives = 547/754 (72.55%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMG--------TDTP----------LAVLSNKP-----------------------QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDIN--------------RGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            MP DI  Y+ DL + D+VT+LALVHQRFSTNT PTW+LAQPFR+MC NGEINTLRGN+SRM+ REE+ +S+ FG  ++K+ PI + GKSDSASMDM +ELL HTGRSLPE MMMM+PEAWEK+  MS E+KAFYEYNACIMEPWDGPAS+PFTDG+YIGALLDRNGLRPSRYTVTK G +IM+SE GV D+ P +VE HGRLEPG+MFLVDM KGRII DEE+K  IV++RPY+EWLN+  L L +IPYT    PIE ++I  RQ+LF YT EDI+ +ITPMA  GKEA+GSMG        +D P           A ++N P                        ++  + K+L  Q   ++N  LD I+                             EE++ +IN              R V+ + APIP+LLACSFVHH++    +RS F I IESAEPREPHHFA LFGYGASA+NPYMVNEII   +        + + A+ NFNKAIGKGI+K+MNKIGISTL SYRGSQIFEI+G N +FV+KYF  T +RIEGIGLYEIE+EI +RY  A+     +  L L+IGGDYRWRRNGERH+FNP +VAKLQQAV+ ++  SY+ Y+K INEQ+E LMT+RGLF+F + +PIP+EEVEPWT+IVKRFKTGAMS+GSIS+EAHENLAIAMNR+ GKSNSGEGGED +RF  D+NG+ RNSAIKQVASGRFGV+ +YL+NA+EIQIKMA
Sbjct:  201 MPNDINIYYPDLNDDDVVTKLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLRGNLSRMKAREELFESEFFGEDLKKIIPITMEGKSDSASMDMALELLLHTGRSLPEAMMMMVPEAWEKNPVMSDEKKAFYEYNACIMEPWDGPASIPFTDGNYIGALLDRNGLRPSRYTVTKDGYVIMASETGVTDIKPGNVEFHGRLEPGRMFLVDMVKGRIIEDEEVKYDIVTKRPYREWLNDNLLPLANIPYTGNKTPIEKVEINKRQKLFGYTLEDIKTIITPMATQGKEAIGSMGADIPLAVLSDQPQLLFNYFKQLFAQVTNPPLDGIREEIVTDISLTIGEDKNLFDIVPEHCKKLRIQNPVISNEDLDKIKYIDHPDFKAKSISMLYEAEKGLNGLEARLEEMIFEINDAVDEGCNIIILSDRDVSDKLAPIPSLLACSFVHHRVKDHDRRSSFGIVIESAEPREPHHFALLFGYGASAINPYMVNEII-FNLANEDEIEEEPEVAIQNFNKAIGKGIVKIMNKIGISTLLSYRGSQIFEILGLNKKFVDKYFCNTPTRIEGIGLYEIEKEIQKRYHKAFYPKTTEVGLDLDIGGDYRWRRNGERHMFNPASVAKLQQAVKQNNPTSYSEYSKLINEQNERLMTLRGLFKFRDLNPIPIEEVEPWTDIVKRFKTGAMSFGSISKEAHENLAIAMNRLKGKSNSGEGGEDADRFHKDLNGNWRNSAIKQVASGRFGVSINYLSNAQEIQIKMA 953          

HSP 2 Score: 158.303 bits (399), Expect = 5.433e-36
Identity = 71/84 (84.52%), Postives = 77/84 (91.67%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L +RI VECDGQLKTGRDVAIA LLGAEEFGF+TAPLVASGCIMMR CHLNTCPVGIATQD ELRKNFKGTPE VIN+ Y++A+
Sbjct: 1081 LRNRITVECDGQLKTGRDVAIAALLGAEEFGFSTAPLVASGCIMMRACHLNTCPVGIATQDPELRKNFKGTPEDVINYMYFVAQ 1164          

BLAST of glutamate synthase vs. nr
Match: gi|496140721|ref|WP_008865228.1| (glutamate synthase large subunit [Flavobacteria bacterium MS024-2A] >gi|225003926|gb|EEG41898.1| Glutamate synthase (ferredoxin) [Flavobacteria bacterium MS024-2A])

HSP 1 Score: 897.886 bits (2319), Expect = 0.000e+0
Identity = 445/757 (58.78%), Postives = 553/757 (73.05%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMG---------------------------------------TDTPLAVLS--NKPQLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI---------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGL--NIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +PEDI  Y++DL +  +VTRLALVHQRFSTNT PTW+LAQPFR+MC NGEINT RGN+SRM+VREE+++SD+FG  I+K+ P+IL GKSDS+SMDMVVELL  TGRSLPEVMMM++PEAWEKH  M   +KAFY+YNACIMEPWDGPAS+PFTDG YIGALLDRNGLRPSRY+VTK G ++MSSE GV+D+APE++E HGRLEPGKMFLV+M++GRII D+EIK+++ ++RPY++WLN   L LK++PYT    P E  D +TR R+F YT ED++ ++TPM   GKEA+GSMG                                       TDT L++ S  N  ++ +N+ K+L  Q   ++N  LD I+                             EE++  I               +RGV+   APIP LLA S  HH + R++KRS F I IESAEPREPHHF+ LFGYGASA+NPYM+NEII  Q KE  I  +  +KA++NFNKAI KGI+KVMNKIGISTL SYRG+QIFE +G + + ++++F  TA+RIEGIGLYEIE+EIN R+  AY   + +  LGL   IGGDYRWRR+GE H+ NP+T++ LQQAVR +   SY  +AK INEQ+E LMT+RGLFEF +FDPIP+++VEPWT+IVKRFKTGAMS GSIS+EAHENLAIAMNRIGGKSNSGEGGED+ RFQPD +G+ RNSAIKQVASGRFGV+SHYL++AKEIQIKMA
Sbjct:  202 IPEDIKGYYQDLSDPAVVTRLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTFRGNLSRMQVREELLESDLFGSDIKKILPVILKGKSDSSSMDMVVELLLATGRSLPEVMMMLVPEAWEKHTAMDDNKKAFYKYNACIMEPWDGPASIPFTDGKYIGALLDRNGLRPSRYSVTKDGYVVMSSETGVLDIAPENIERHGRLEPGKMFLVNMDEGRIIEDDEIKNEVTTKRPYKKWLNENQLPLKEVPYTGNVTPTEKEDFETRMRIFGYTQEDLKTILTPMITEGKEAIGSMGIDTPLAVLSERPQLLYNYFKQLFAQVTNPPLDGIREEIVTDTSLSIGSEFNLFEISANHAKKLSIQNPVISNEDLDKIKFIESENFKSATISTLYEISGRLNSLENALEEMILAIEKAIDHEGANIIILSDRGVSPSMAPIPMLLATSHAHHALKRIKKRSKFGIIIESAEPREPHHFSLLFGYGASAINPYMINEIIFNQAKESVIN-IPAEKAIENFNKAIAKGIVKVMNKIGISTLHSYRGAQIFEALGLSQKVIDRFFCNTATRIEGIGLYEIEKEINSRHTKAY---IHESNLGLPLEIGGDYRWRRDGEEHVVNPSTISNLQQAVRQNKPASYAAFAKEINEQNEKLMTLRGLFEFSSFDPIPIDQVEPWTDIVKRFKTGAMSLGSISQEAHENLAIAMNRIGGKSNSGEGGEDKLRFQPDKDGNWRNSAIKQVASGRFGVSSHYLSSAKEIQIKMA 954          

HSP 2 Score: 161.77 bits (408), Expect = 4.720e-37
Identity = 71/84 (84.52%), Postives = 78/84 (92.86%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SRIV+ECDGQ+KTGRDVAIA LLGAEEFGF+TAPL+ASGCIMMR CHLNTCPVGIATQD ELRKNFKG PEHVIN+ Y++AE
Sbjct: 1082 LRSRIVLECDGQMKTGRDVAIACLLGAEEFGFSTAPLIASGCIMMRACHLNTCPVGIATQDPELRKNFKGKPEHVINYMYFVAE 1165          

BLAST of glutamate synthase vs. nr
Match: gi|1200282037|gb|OUU04387.1| (glutamate synthase large subunit [Flavobacteriaceae bacterium TMED42])

HSP 1 Score: 895.96 bits (2314), Expect = 0.000e+0
Identity = 447/753 (59.36%), Postives = 545/753 (72.38%), Query Frame = 0

Query:    2 PEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVI-----------------TPMAVVG----------KEALGSMG------------TDTPLAVLS--NKPQLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            PEDI  Y+KDL +  +VTRLALVHQRFSTNT PTW+LAQPFR+MC NGEINT RGN SRM+ REE+  S  FG  I+K+ P++LPGKSDS+SMDM VELL  TGRSLPEVMMMM+PEAWE+H +M  ++KAFYEYNACIMEPWDGPAS+PFTDG YIGALLDRNGLRPSRYTVTK G ++MSSE GV+D+ P++VE HGRLEPG+MFLV+M+ GRII D EIK +IV ++PY++WLN   L LK++PYT    P+E  + +TR RLF YT ED++ +I                 TP+AV+           K+    +             TDT L++ S  N  +++ +  K+L  Q   ++N  LD I+                             ++++ +I              +RGV+ E APIP LLACS  HH + +L+KRS F I IESAEPREPHHF+ LFGYGASA+NPYMVNEII  QV+   I G+  +KA+DNFNKAI KGI+KVMNKIGISTL SYRG+QIFE +G N +F+E YF  TA+RIEGIGLYEIE+EIN+R+  AY        + L IGGDYRWRR+GE+H+ NP ++AKLQQAVR +  E+Y VY+K INEQSE LMT+RG+FEF +FDPIPLEEVEPWTEIVKRFKTGAMS GSIS EAHENLAIAMNRIGGKSNSGEGGED  RFQPD++G+ +NSAIKQVASGRFGV+SHYL++AKEIQIKMA
Sbjct:  203 PEDINAYYKDLNDPLVVTRLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTFRGNYSRMQTREELFASAAFGDDIKKILPVVLPGKSDSSSMDMAVELLLATGRSLPEVMMMMVPEAWERHLSMDEDKKAFYEYNACIMEPWDGPASIPFTDGKYIGALLDRNGLRPSRYTVTKDGYVVMSSEAGVLDIEPDNVEKHGRLEPGRMFLVNMDDGRIIEDGEIKKEIVGKKPYRKWLNENLLELKEVPYTGNKTPVEKENFETRLRLFGYTEEDMKTIINPMSVSGKEAIGSMGTDTPLAVLSDRPQLIYNYFKQLFAQVTNPPLDGIREEIVTDTSLSIGSDYNIFEVVPDQCKKLRIQNPVISNEDLDKIKYISHKDYKAVSINTLYEVAKGQNGLEAALDQMIVEIEKAVDVGANIIILSDRGVSPERAPIPMLLACSHAHHGLKKLKKRSLFGIVIESAEPREPHHFSLLFGYGASAINPYMVNEIITHQVENDIIQGISVEKAIDNFNKAIAKGIVKVMNKIGISTLHSYRGAQIFEALGLNEKFIETYFCKTATRIEGIGLYEIEKEINKRFHKAYSQENF-AGMPLEIGGDYRWRRDGEKHMLNPASIAKLQQAVRQNKFETYGVYSKLINEQSEKLMTLRGMFEFSSFDPIPLEEVEPWTEIVKRFKTGAMSLGSISAEAHENLAIAMNRIGGKSNSGEGGEDPQRFQPDMDGNWKNSAIKQVASGRFGVSSHYLSSAKEIQIKMA 954          

HSP 2 Score: 162.155 bits (409), Expect = 3.494e-37
Identity = 72/84 (85.71%), Postives = 78/84 (92.86%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SRIV+ECDGQ+KTGRDVAIA LLGAEEFGF+TAPLVASGCIMMR CHLNTCPVGIATQD ELRKNFKG PEHVINF Y++A+
Sbjct: 1082 LRSRIVLECDGQMKTGRDVAIACLLGAEEFGFSTAPLVASGCIMMRACHLNTCPVGIATQDPELRKNFKGKPEHVINFMYFVAQ 1165          

BLAST of glutamate synthase vs. nr
Match: gi|1103708762|ref|WP_071671758.1| (glutamate synthase large subunit [Flavobacteriaceae bacterium UJ101] >gi|1102954639|gb|APD07939.1| glutamate synthase (ferredoxin) [Flavobacteriaceae bacterium UJ101])

HSP 1 Score: 883.634 bits (2282), Expect = 0.000e+0
Identity = 449/756 (59.39%), Postives = 545/756 (72.09%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVI-----------------TPMAVVGKE-------------------------------ALGSMGTDTPLAVLS---------NKPQLISNY-------FKQLFAQVTNPP--------LDGIREEIVTDIN-----------------RGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +PEDI  Y+KDLQE D++TRLALVHQRFSTNT PTW+LAQPFR MC NGEINTLRGNVSRMR REE++KSD     I+++ P IL GKSDSASMDMV+ELL  TGRSLPE MMM++PEAWEKHQ+MS  +KAFYEYNACIMEPWDGPAS+PFTDG+YIGALLDRNGLRPSRYTVTKSG +IMSSE GV+++ PE++E HGRLEPGKMFLVDMN+GRI+NDEEIK +I S+ PYQ+WL   +++L DIPYT    P E  D++ R+++F YT+E+I ++I                 TP+AV   +                               +LG +GTD  L  ++         N P +IS Y       +  L  + T  P        L+G+ E + T IN                 RG  +  APIPALLACS VHH + R  KRS F I +ESAEPRE H FA LFGYGASA+NPYMVNEII  +V    +  +D +KA++N+NKAIGKG++K+MNKIGISTL SYRG+QIFE +G N +F+ KYFPYT +RIEGIGLYEIE+E+ +R+K A+  +  +  L L IGGDYRWRRNGE+HLFNP T+AKLQQAVR ++ +SY  Y+K IN+QS+  MTIRGL EF N DPI ++EVEPWTEIVKRFKTGAMSYGSIS+EAHE LAIAMNR+GGKSNSGEGGE+  RF  D +GD RNSAIKQVASGRFGV+S+YLT+A+EIQIKMA
Sbjct:  201 IPEDIKLYYKDLQEEDVITRLALVHQRFSTNTFPTWDLAQPFRQMCHNGEINTLRGNVSRMRAREELLKSDSIEEDIKEILPTILNGKSDSASMDMVLELLLITGRSLPEAMMMLVPEAWEKHQSMSTTKKAFYEYNACIMEPWDGPASIPFTDGNYIGALLDRNGLRPSRYTVTKSGYVIMSSETGVIELEPENIEYHGRLEPGKMFLVDMNEGRIVNDEEIKEQIASKYPYQKWLKENTVHLTDIPYTQCIIPNEKTDLQIRKKVFGYTYEEITKIIAPMASLGKEPIGSMGTDTPLAVFSHKPQLLYNYFKQLFAQVTNPPLDGIREELITDLSLG-LGTDYNLFEITENHCKKLRINNP-IISKYDLEKIKTYDNLDFKSTTIPILYEVKEGLNGLEEALDTLINQVEKAVEENNTIIVLSDRGTTKNLAPIPALLACSLVHHNLQRRGKRSRFSIIMESAEPREVHQFALLFGYGASAINPYMVNEIIEERVDMNTVK-LDSEKAIENYNKAIGKGVVKIMNKIGISTLNSYRGAQIFEALGLNKKFITKYFPYTPTRIEGIGLYEIEKELYKRHKEAFEEHQ-NGNLNLEIGGDYRWRRNGEKHLFNPLTIAKLQQAVRQNEPQSYAEYSKLINDQSKQTMTIRGLLEFANHDPISIDEVEPWTEIVKRFKTGAMSYGSISQEAHETLAIAMNRLGGKSNSGEGGENPQRFYKDQSGDWRNSAIKQVASGRFGVSSNYLTSAQEIQIKMA 952          

HSP 2 Score: 159.073 bits (401), Expect = 2.894e-36
Identity = 70/84 (83.33%), Postives = 77/84 (91.67%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L +RIVVECDGQLKTGRDVAIA LLGAEEFGFATAPLVA+GC+MMR CHLNTCPVGIATQ+ ELRK FKG PEHV+NF YY+A+
Sbjct: 1080 LRNRIVVECDGQLKTGRDVAIACLLGAEEFGFATAPLVATGCVMMRACHLNTCPVGIATQNPELRKKFKGKPEHVVNFMYYVAQ 1163          

BLAST of glutamate synthase vs. nr
Match: gi|1251805668|gb|PDH43581.1| (glutamate synthase large subunit [Flavobacteriales bacterium MED-G22])

HSP 1 Score: 878.241 bits (2268), Expect = 0.000e+0
Identity = 442/756 (58.47%), Postives = 541/756 (71.56%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVI-----------------TPMAVVG----------KEALGSMG------------TDTPLAVLS--NKPQLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLG--LNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +PEDI  Y+ DL    +VTRLALVHQRFSTNT PTW+LAQPFR+MC NGEINT RGN SRM+ REE++KSD+FG  I+K+ P+ILP KSDSASMDMVVELL  +GRSLPEVMMMM+PEAWEKHQ M  ++KAFYEYN+CIMEPWDGPAS+PFTDG YIGALLDRNGLRPSRY+VTK G ++MSSE GV+D+AP+++E HGRLEPGKMFLV+MN+GRI+ DEEIK +I SERPY+EWL    L LK++PYT    P E  D +TR R+F YT ED++ +I                 TP+AV+           K+    +             TDT L+V S  N   + +N+ K+L  Q   ++N  LD I+                             EE+V  +              +RGV+   APIP LLAC+  H+ + R  KRS F I +ESAEPREPHHFA LFGYGASA+NPY+VNE+I+ Q +   I  ++  KA+ NFNKAI KGI+KVMNKIGISTL SYRG+QIFE +G + +F++++F  TA+RIEGIGLYEIE+EI +R+  AY   L +  +G  L IGGDYRWRR+GE H  NP TVA LQQAVR +  E Y  +AK +NEQ++ LMT+RGLFEF +FDPIPL++VEPWT IVKRFKTGAMS GSIS+EAHENLA+AMNRIGGKSNSGEGGED  RFQPD++G+ RNSAIKQVASGRFGV+SHYL++AKEIQIKMA
Sbjct:  201 IPEDIKNYYLDLSNPAVVTRLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTFRGNYSRMQAREELLKSDLFGEDIKKILPVILPSKSDSASMDMVVELLLASGRSLPEVMMMMVPEAWEKHQAMDEDKKAFYEYNSCIMEPWDGPASIPFTDGKYIGALLDRNGLRPSRYSVTKDGYVVMSSETGVLDIAPDNIERHGRLEPGKMFLVNMNEGRIVEDEEIKKEITSERPYREWLQKNLLRLKEVPYTGNKTPDEKEDFETRLRIFGYTQEDLKTIIKPMVISAKEAIGSMGTDTPLAVLSDKPQLLYNYFKQLFAQVTNPPLDGIREEIVTDTSLSVGSDYNIFDVTANHAKKLHIQNPVISNEDLDKIKNIENPDFKSVSISTLYETQSGLNGLENALEEMVHQVERALSNGANIIILSDRGVSSSLAPIPMLLACAHTHNALKRKGKRSRFGIILESAEPREPHHFALLFGYGASAINPYLVNEVIQHQTENEGIA-LETDKAISNFNKAIAKGIVKVMNKIGISTLHSYRGAQIFEALGLSQKFIDRFFCNTATRIEGIGLYEIEKEIAQRHSKAY---LHESNIGMSLEIGGDYRWRRDGEAHAVNPHTVAHLQQAVRQNKVEEYETFAKLVNEQNKKLMTLRGLFEFSSFDPIPLDQVEPWTAIVKRFKTGAMSLGSISQEAHENLAVAMNRIGGKSNSGEGGEDPKRFQPDIDGNWRNSAIKQVASGRFGVSSHYLSSAKEIQIKMA 952          

HSP 2 Score: 161.77 bits (408), Expect = 4.430e-37
Identity = 71/84 (84.52%), Postives = 78/84 (92.86%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SRIV+ECDGQ+KTGRDVAIA LLGAEEFGF+TAPL+ASGCIMMR CHLNTCPVGIATQD ELRKNFKG PEHVIN+ Y++AE
Sbjct: 1080 LRSRIVLECDGQMKTGRDVAIACLLGAEEFGFSTAPLIASGCIMMRACHLNTCPVGIATQDPELRKNFKGKPEHVINYMYFVAE 1163          

BLAST of glutamate synthase vs. nr
Match: gi|1200589957|gb|OUW99846.1| (glutamate synthase large subunit [Flavobacteriaceae bacterium TMED238])

HSP 1 Score: 874.004 bits (2257), Expect = 0.000e+0
Identity = 433/755 (57.35%), Postives = 536/755 (70.99%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQ-IEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTRQRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTP------------------LAVLSNKP-----------------------QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            +PEDI  ++ DL    LVT LALVHQRFSTNT PTW+LAQPFR+MC NGEINTLRGNVSRM  REE++ S +   + + K+FPII+PGKSDSASMDMV+ELL  TGRSLPE+MM+++PEAWEKH++M+  +KAFYE+N+C+MEPWDGPAS+PFTDG YIGALLDRNGLRPSRYTVTK G ++MSSE GV+++ PE++E HGRLEPGKMFLVDMN GRII DEEIK +IVS+ PY++W+N+  L L  IPYT    P E I+ +TR ++F YT ED   +I PM   GKE++GSMG+DTP                   A ++N P                        LI  + K+L  Q   ++N  LD I+                             E IV D+              +R VN++ APIP LLACSF+HH M + +KRS F I IESAEPREPHHF+ LFGYGASA+NPY+VNEII    + G I  +   +AV N+N A  KGILKVMNKIGISTL SYRG+QIFE +G  S+FV+KYF  T SRIEG+GLY +E+EI+ R K A+ +   D+ + +  GG+Y+WRRNGE H+ NP T++KLQQ+VR    E+Y +Y+ +IN+Q+E  MT+RG+F+F  FDPIP+EEVE WTEIVKRFKTGAMSYGS+S+EAHENLAIAMNRIGGKSNSGEGGED  RF+ + NGDS+NS+IKQVASGRFGV+SHYLTNA EIQIKMA
Sbjct:  202 IPEDIERFYIDLSNPLLVTSLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLRGNVSRMISREELINSKLISEEFLNKIFPIIIPGKSDSASMDMVLELLLMTGRSLPEIMMILVPEAWEKHKSMNDSKKAFYEFNSCLMEPWDGPASIPFTDGKYIGALLDRNGLRPSRYTVTKDGYVVMSSETGVIEIKPENIEKHGRLEPGKMFLVDMNAGRIIEDEEIKKEIVSKHPYEKWVNDNVLPLSKIPYTGNRTPKEKINFETRLKIFGYTKEDFNTIIIPMCKKGKESIGSMGSDTPLAILSKRPQLLYNYFKQLFAQVTNPPLDGIREEIVTDTSLSVGSDYNIFDLIPEHAKKLKIQNPIISNEDLDKIKYIDHHDFKASSVKALYKLSKGHNGLEEALESIVNDVEKKVDNGSNIIIISDRNVNKKLAPIPILLACSFIHHSMIKRKKRSNFGIVIESAEPREPHHFSMLFGYGASAINPYLVNEIISFHHELGIIKNISLDQAVSNYNNATAKGILKVMNKIGISTLHSYRGAQIFEALGLRSKFVKKYFNNTPSRIEGVGLYVLEKEISTRLKKAFESK--DEFINIETGGEYKWRRNGEAHMLNPLTISKLQQSVRDESYETYKIYSDSINKQNEQFMTLRGMFKFSKFDPIPIEEVESWTEIVKRFKTGAMSYGSLSKEAHENLAIAMNRIGGKSNSGEGGEDPLRFKLNQNGDSKNSSIKQVASGRFGVSSHYLTNATEIQIKMA 954          

HSP 2 Score: 158.303 bits (399), Expect = 5.823e-36
Identity = 71/84 (84.52%), Postives = 76/84 (90.48%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SR+VVECDGQLKTGRDVAIA LLGAEEFGF+TAPLVASGCIMMR CHLNTCPVGIAT D  LRKNFKG PEHVIN+ Y+IA+
Sbjct: 1082 LRSRVVVECDGQLKTGRDVAIACLLGAEEFGFSTAPLVASGCIMMRACHLNTCPVGIATTDPVLRKNFKGKPEHVINYMYFIAQ 1165          

BLAST of glutamate synthase vs. nr
Match: gi|1173355557|ref|WP_081211785.1| (glutamate synthase large subunit [Salegentibacter sp. K5023])

HSP 1 Score: 871.307 bits (2250), Expect = 0.000e+0
Identity = 441/757 (58.26%), Postives = 541/757 (71.47%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTR------------------------------------------QRLFNYTFEDIQEVITPMAVVGKEALGSMGTDTPLAVLSNKP--QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDIN--------------RGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            MP+DI  Y+KDL E  +VT+LALVHQRFSTNT PTW+LAQPFR+MC NGEINTLRGN+SRM+ REE+ +S+VFG  ++K+ PI   GKSDSASMD V+ELL  TGRSLPE +MMM+PEAWEK+ +MS E+KAFYEYNACIMEPWDGPAS+PFTDG+YIGALLDRNGLRPSRYT+TK G +IMSSE GV+D+ PE+VE HGRLEPG+MFLVDMN+GRI+ DEE+KSKIVSERPY++WLNN  L L ++PYT    P E ID  TR                                          Q LFNY  +   +V  P     +E    + TD  LA+  ++    +I    K+L  Q   ++N  LD I+                             +EI+ +IN              R VN + APIP+LLACSFVHH++ +  +RS F I IESAEPREPHHFA LFGYGASA+NPYMVNEII   V++  I   D ++AV NFNKAIGKGI+K+MNKIGISTL SYRG+QIFEI+G N +FV+KYF  T +RIEGIGLYEIE+EI +R++ A+     D  L L+IGGDYRWRRNGERH+FNP +VAKLQQAV+ ++ +SY  Y+K IN+Q+E LMT+RG+F+F + +PIP++EVEPWTEIVKRFKTGAMS+GSIS+EAHENLAIAMNR+ GKSNSGEGGED  RF  D++G+ RNSAIKQVASGRFGV+ +YL++A EIQIKMA
Sbjct:  201 MPQDINVYYKDLNEPGVVTKLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLRGNLSRMKAREELFESEVFGEDLKKIVPITADGKSDSASMDTVLELLLQTGRSLPEAIMMMVPEAWEKNPSMSEEKKAFYEYNACIMEPWDGPASIPFTDGNYIGALLDRNGLRPSRYTLTKDGYVIMSSETGVMDIKPENVELHGRLEPGRMFLVDMNEGRIVRDEEVKSKIVSERPYRKWLNNNLLPLAEVPYTGNKTPREKIDFNTRQKLFGYTLEDISTIITPMATLGKEAIGSMGADIPLAVLSDKPQLLFNYFKQLFAQVTNPPLDGIRE---EIVTDISLAIGEDRNIFDIIPEQAKKLRIQNPVISNEDLDKIKYIDQPDFKTESISILYEAEKGLNGLETRLDEIIYEINNAVDRGCNVIILSDRNVNPKLAPIPSLLACSFVHHRVKKYNRRSSFGIVIESAEPREPHHFALLFGYGASAINPYMVNEIIHQLVEDNEIDMEDAEEAVQNFNKAIGKGIVKIMNKIGISTLHSYRGAQIFEILGLNQKFVDKYFSNTPTRIEGIGLYEIEKEIQKRFEAAFQPPETDTGLDLDIGGDYRWRRNGERHMFNPASVAKLQQAVKQNNPKSYQEYSKLINDQNEALMTLRGMFKFKDLNPIPIDEVEPWTEIVKRFKTGAMSFGSISKEAHENLAIAMNRMKGKSNSGEGGEDPQRFNKDLDGNWRNSAIKQVASGRFGVSINYLSSASEIQIKMA 954          

HSP 2 Score: 159.458 bits (402), Expect = 2.149e-36
Identity = 73/84 (86.90%), Postives = 77/84 (91.67%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L +RIVVECDGQLKTGRDVAIA LLGAEEFGF+TAPLVASGCIMMR CHLNTCPVGIATQD ELRK FKGTPE VINF Y+IA+
Sbjct: 1082 LRNRIVVECDGQLKTGRDVAIACLLGAEEFGFSTAPLVASGCIMMRACHLNTCPVGIATQDPELRKKFKGTPEDVINFMYFIAQ 1165          

BLAST of glutamate synthase vs. nr
Match: gi|1124702267|ref|WP_074980269.1| (glutamate synthase large subunit [Zhouia amylolytica] >gi|1097571495|emb|SFT17069.1| glutamate synthase (ferredoxin) [Zhouia amylolytica])

HSP 1 Score: 868.611 bits (2243), Expect = 0.000e+0
Identity = 438/758 (57.78%), Postives = 539/758 (71.11%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETI--------------DIKT----------------------------RQRLFNYTFEDIQEVITP-MAVVGKEALGSMGTDTPLAVLSNKP--QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            MP+DI  Y+ DL + D+VT+LALVHQRFSTNT PTW+LAQPFR+MC NGEINTL GN+SRM+ REE+  S +FG  ++ + P++L GKSDSASMDMV+ELL  TGRSLPE MMM++PEAWE+HQ+M   +KAFYEYNACIMEPWDGPAS+PFTDG+YIGALLDRNGLRPSRY+VTK G +IMSSE GVVDV P+++E HGRLEPGKMFLVDMN+GRII DEE+K  IVS+RPY++W++   L L  I YT    P+E +              DIKT                             Q LFNY  +   +V  P +  + +E +    TD  L++ ++K    +I    K+L  Q   ++N  LD I+                             E+I+ +I              +RGV++  APIP+LLACSFVHH + + +KRS   I +ESAEPREPHHFATLFGYGASA+NPYMVNEII   V EG I G++   A+ NFNKAIGKGI+K+MNKIGISTL SYRGSQIFE +G N +FV KYF  T++RIEGIGLYEIE+EI +R++ A+ N+ I   + L IGGDYRWRRNGERH+FNP +VAKLQQ+VR +  ++Y  YA  INEQSE LMT+RG+F+F + +PI ++EVEPWT+IVKRFKTGAMS+GSISREAHENLAIAMNRIGGKSNSGEGGED  RFQ D+NG+ RNSAIKQVASGRFGV+  YL+NA+EIQIKMA
Sbjct:  202 MPQDISGYYLDLLDPDVVTKLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLTGNLSRMKAREELFASKLFGEDLKHIPPVVLDGKSDSASMDMVLELLLQTGRSLPEAMMMLVPEAWERHQSMPDNKKAFYEYNACIMEPWDGPASIPFTDGNYIGALLDRNGLRPSRYSVTKDGYVIMSSETGVVDVEPDNIEYHGRLEPGKMFLVDMNEGRIIEDEEVKETIVSKRPYRQWVDENLLPLAKIAYTGNKTPVEAVDPITREKLFGYTQEDIKTIISPMAIGAKEAIGSMGTDTPLAVLSDKPQLLFNYFKQLFAQVTNPPLDGIREEIV----TDISLSIGADKNIFDVIPEQAKKLRIQNPVISNEDLDKIKYIEHPDFKTKSISILYEAAKGLNGLESRLEDIIKEISTAIDEGFNIIILSDRGVSETMAPIPSLLACSFVHHGLKKHKKRSSMGIIVESAEPREPHHFATLFGYGASAINPYMVNEIIANLVDEGEINGIEAPVAIQNFNKAIGKGIIKIMNKIGISTLHSYRGSQIFEALGLNEKFVNKYFCNTSTRIEGIGLYEIEKEIQKRFQKAFSNSQIGAEMDLEIGGDYRWRRNGERHMFNPASVAKLQQSVRQNRYDTYKEYASLINEQSERLMTLRGMFKFKDLNPISIDEVEPWTDIVKRFKTGAMSFGSISREAHENLAIAMNRIGGKSNSGEGGEDPGRFQKDLNGNWRNSAIKQVASGRFGVSIDYLSNAREIQIKMA 955          

HSP 2 Score: 166.007 bits (419), Expect = 1.857e-38
Identity = 75/84 (89.29%), Postives = 79/84 (94.05%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L +RIVVECDGQLKTGRDVAIA LLGAEEFGFATAPLVASGCIMMR CHLNTCPVGIATQD ELRKNFKGTPEHVIN+ Y+IA+
Sbjct: 1083 LRNRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRACHLNTCPVGIATQDPELRKNFKGTPEHVINYMYFIAQ 1166          

BLAST of glutamate synthase vs. nr
Match: gi|740427976|ref|WP_038260517.1| (glutamate synthase large subunit [Zhouia amylolytica] >gi|568805140|gb|ETN96592.1| glutamate synthase family protein [Zhouia amylolytica AD3])

HSP 1 Score: 867.07 bits (2239), Expect = 0.000e+0
Identity = 439/758 (57.92%), Postives = 537/758 (70.84%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETI--------------DIKT----------------------------RQRLFNYTFEDIQEVITP-MAVVGKEALGSMGTDTPLAVLSNKP--QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            MP+DI  Y+ DL + D+VT+LALVHQRFSTNT PTW+LAQPFR+MC NGEINTL GN+SRM+ REE+  S +FG  ++ + PI+L GKSDSASMDMV+ELL  TGRSLPE MMM++PEAWE+HQ+M   +KAFYEYNACIMEPWDGPAS+PFTDG+YIGALLDRNGLRPSRY+VTK G +IMSSE GVVDV PE++E HGRLEPGKMFLVDMN+GRII DEE+K  IVS+RPY++W++   L L  I YT    P+E +              DIKT                             Q LFNY  +   +V  P +  + +E +    TD  L++ ++K    +I    K+L  Q   ++N  LD I+                             E+I+ +I              +RGV++  APIP+LLACS+VHH + + +KRS   I IESAEPREPHHFATLFGYGASA+NPYMVNEII   V EG I G++   A+ NFNKAIGKGI+K+MNKIGISTL SYRGSQIFE +G N +FV KYF  T++RIEGIGLYEIE+EI +R++ A+    I   + L IGGDYRWRRNGERH+FNP +VAKLQQ+VR +  ++Y  YA  INEQSE LMT+RG+F+F + +PI ++EVEPWT+IVKRFKTGAMS+GSISREAHENLAIAMNRIGGKSNSGEGGED  RFQ D+NG+ RNSAIKQVASGRFGV+  YL+NA+EIQIKMA
Sbjct:  202 MPQDISGYYLDLLDPDVVTKLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLTGNLSRMKAREELFASKLFGEDLKHIPPIVLDGKSDSASMDMVLELLLQTGRSLPEAMMMLVPEAWERHQSMPDNKKAFYEYNACIMEPWDGPASIPFTDGNYIGALLDRNGLRPSRYSVTKDGYVIMSSETGVVDVEPENIEYHGRLEPGKMFLVDMNEGRIIEDEEVKETIVSKRPYRQWVDENLLPLAKIAYTGNKTPVEAVDPITREKLFGYTQEDIKTIISPMAIGAKEAIGSMGTDTPLAVLSDKPQLLFNYFKQLFAQVTNPPLDGIREEIV----TDISLSIGADKNIFDVIPEQAKKLRIQNPVISNEDLDKIKYIEHPDFKTKSISILYEAAKGLNGLESRLEDIIKEISTAIDEGFNIIILSDRGVSETMAPIPSLLACSYVHHGLKKHKKRSSVGIIIESAEPREPHHFATLFGYGASAINPYMVNEIIANLVDEGEINGIEAPVAIQNFNKAIGKGIIKIMNKIGISTLHSYRGSQIFEALGLNEKFVNKYFSNTSTRIEGIGLYEIEKEIQKRFQKAFSKRQIGAEMDLEIGGDYRWRRNGERHMFNPASVAKLQQSVRQNRYDTYKEYASLINEQSERLMTLRGMFKFKDLNPISIDEVEPWTDIVKRFKTGAMSFGSISREAHENLAIAMNRIGGKSNSGEGGEDPGRFQKDLNGNWRNSAIKQVASGRFGVSIDYLSNAREIQIKMA 955          

HSP 2 Score: 166.007 bits (419), Expect = 1.840e-38
Identity = 75/84 (89.29%), Postives = 79/84 (94.05%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L +RIVVECDGQLKTGRDVAIA LLGAEEFGFATAPLVASGCIMMR CHLNTCPVGIATQD ELRKNFKGTPEHVIN+ Y+IA+
Sbjct: 1083 LRNRIVVECDGQLKTGRDVAIAALLGAEEFGFATAPLVASGCIMMRACHLNTCPVGIATQDPELRKNFKGTPEHVINYMYFIAQ 1166          

BLAST of glutamate synthase vs. nr
Match: gi|1183603860|ref|WP_084841254.1| (glutamate synthase large subunit [Zunongwangia atlantica] >gi|1181192801|gb|ORL45662.1| glutamate synthase (NADPH) large subunit [Zunongwangia atlantica 22II14-10F7])

HSP 1 Score: 866.3 bits (2237), Expect = 0.000e+0
Identity = 441/758 (58.18%), Postives = 534/758 (70.45%), Query Frame = 0

Query:    1 MPEDIGPYFKDLQETDLVTRLALVHQRFSTNTMPTWELAQPFRFMCQNGEINTLRGNVSRMRVREEIMKSDVFGPQIEKLFPIILPGKSDSASMDMVVELLTHTGRSLPEVMMMMIPEAWEKHQTMSPERKAFYEYNACIMEPWDGPASVPFTDGDYIGALLDRNGLRPSRYTVTKSGKLIMSSEIGVVDVAPEDVESHGRLEPGKMFLVDMNKGRIINDEEIKSKIVSERPYQEWLNNTSLNLKDIPYTNQTCPIETIDIKTR------------------------------------------QRLFNYTFEDIQEVITP-MAVVGKEALGSMGTDTPLAVLSNKP--QLISNYFKQLFAQ---VTNPPLDGIR-----------------------------EEIVTDI--------------NRGVNQEFAPIPALLACSFVHHQMNRLRKRSYFDIFIESAEPREPHHFATLFGYGASAVNPYMVNEIIRMQVKEGFITGMDEQKAVDNFNKAIGKGILKVMNKIGISTLRSYRGSQIFEIVGFNSQFVEKYFPYTASRIEGIGLYEIEREINERYKYAYPNNLIDKRLGLNIGGDYRWRRNGERHLFNPTTVAKLQQAVRLSDQESYNVYAKAINEQSENLMTIRGLFEFDNFDPIPLEEVEPWTEIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGEDRNRFQPDVNGDSRNSAIKQVASGRFGVTSHYLTNAKEIQIKMA 667
            MP+DI  Y+KDL + DLVT+LALVHQRFSTNT PTW+LAQPFR+MC NGEINTLRGN+SRM+ REE+ +S++FG  I+K+ P+ L GKSDSASMDMV+E+L  TGRSLPE +MMM+PEAWEK+ +M   +KAFYEYNACIMEPWDGPAS+PFTDG YIGALLDRNGLRPSRYTVTK G +IMSSE GVVDV PE+VE HGRLEPGKMFLVDM +GRI+ DEE+K +IVS+RPY++WL++  L L D+PYT    PIE +D KTR                                          Q LFNY  +   +V  P +  + +E +    TD  L++  +K    +   + K+L  Q   ++N  LD I+                             EEIV +I              +R V+ + APIP+LLACSFVHH++ + ++RS F I IESAEPREPHHFA LFGYGA A+NPYMVNEII    ++G I   D   AV NFNKAIGKGI+K+MNKIGISTL SYRGSQIFEI+G N +F  KYF  T SRIEGIGLYEIE+EI +RYK A+     D  L L IGGDYRWRRNGERH+ NP +VAKLQQAV+ +  ++Y  Y+  IN Q++NLMT+RG+F+F   +PI ++EVEPWTEIVKRFKTGAMS+GSIS+EAHENLAIAMNR+ GKSNSGEGGED NRF  D NG+ RNSAIKQVASGRFGV+ +YL+NAKEIQIKMA
Sbjct:  201 MPQDINIYYKDLNDPDLVTKLALVHQRFSTNTFPTWDLAQPFRYMCHNGEINTLRGNLSRMKAREELFESEIFGEDIKKILPVTLEGKSDSASMDMVLEMLLQTGRSLPEAIMMMVPEAWEKNPSMGDGKKAFYEYNACIMEPWDGPASIPFTDGQYIGALLDRNGLRPSRYTVTKDGYVIMSSETGVVDVKPENVELHGRLEPGKMFLVDMVEGRIVGDEEVKEQIVSKRPYRKWLDSNLLPLADVPYTGNKTPIEPVDFKTRQKVFGYTIEDIKTIISPMATQGKEAIGSMGTDIPLAVLSDKPQLLFNYFKQLFAQVTNPPLDGIREEIV----TDISLSIGEDKNIFDITPEHAKKLRIQNPVISNEDLDKIKYIDHPDFRAKSISILYEIDKGLNGLEERLEEIVQEIRKAVDEDRNIIILSDRNVSDKLAPIPSLLACSFVHHELKKHKRRSAFGIVIESAEPREPHHFALLFGYGACAINPYMVNEIIYKLSEDGDIDEKDPDVAVKNFNKAIGKGIVKIMNKIGISTLHSYRGSQIFEILGLNKKFTNKYFTNTPSRIEGIGLYEIEKEIQKRYKTAFTPPETDTGLDLEIGGDYRWRRNGERHMLNPASVAKLQQAVKQNSYKTYQEYSNFINNQTKNLMTLRGMFKFKELNPISIDEVEPWTEIVKRFKTGAMSFGSISKEAHENLAIAMNRLQGKSNSGEGGEDANRFTRDANGNWRNSAIKQVASGRFGVSINYLSNAKEIQIKMA 954          

HSP 2 Score: 161.384 bits (407), Expect = 5.216e-37
Identity = 73/84 (86.90%), Postives = 78/84 (92.86%), Query Frame = 0

Query:  696 LLSRIVVECDGQLKTGRDVAIATLLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGTPEHVINFFYYIAE 779
            L SRI VECDGQLKTGRDVAIA LLGAEEFGF+TAPLVASGCIMMR CHLNTCPVGIATQD ELRKNFKGTPE+VINF Y++A+
Sbjct: 1082 LRSRITVECDGQLKTGRDVAIACLLGAEEFGFSTAPLVASGCIMMRACHLNTCPVGIATQDPELRKNFKGTPENVINFMYFVAQ 1165