BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000011396 (supercontig:LSalAtl2s:LSalAtl2s787:62044:87799:1 gene:EMLSAG00000011396 transcript:EMLSAT00000011396 description:"maker-LSalAtl2s787-augustus-gene-0.76")

HSP 1 Score: 668.692 bits (1724), Expect = 0.000e+0
Identity = 318/632 (50.32%), Postives = 440/632 (69.62%), Query Frame = 0

Query:  260 EIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRP---QLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACA--TNNYS-----------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKSL--QDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            E  + G  T+ + F  +  +Y E+D LGTREI  EE+E+Q +GK + KY+LGDY + +++ ++ +   ++ GL +LG+E  S++AIL+++R EW++   A F+ +  +VTLYTNLGND +   +NETEV +++ S+E + K + +L AC  +K V+++   + KK       Q   Q I Y  LL        +R  P   D AIIMYTSGSTG PKGVM++H + ++ ++++I+  +F   DRY+AYLPLAH++E++AE +C+  G+KIGYSSP TLT+  SKVK GCKGDAN+ +PTLMC+VP++L+RIYK I+D M+++GWA+EELF+YF++YK+KW+ RGFD PLLNKTLFR +RYFLGGRVRL+L+GGAP+S ETH  TRT + VPVLQ YGLTE +  A  +N Y                  VNW++GGY ++DKPF RGEIH GG+ +  GY+K PE+   DFYEEDG+RWF+TGDIG+++ DGVLK++DR KDLVKL  GEYIS GKVESILK C  +ENIC  AD  KDYL AI +PDK  +    ++ L  +D  KDP  +  V  EI D+G + GL + E+P KI+   GEWT ++GL+TAA K RRR IV  YR Q++K
Sbjct: 1891 EFVKSGYKTLYEAFEDVVDKYGERDVLGTREIFSEENELQPDGKVFKKYLLGDYSYISYNGLRRQTSDLADGLMDLGIEPRSRIAILSESRQEWVVSMYAAFKNNYTLVTLYTNLGNDAVEEGLNETEVPLVIVSDEVLPKFKGILEACPLIKYVVVIEAGNGKKSDLSVNYQNNFQFIMYRDLLKRRSKKKYNRSIPSTNDTAIIMYTSGSTGRPKGVMVNHGDAIAGLRSVISYTDFTTSDRYLAYLPLAHIMEIVAEMACLYCGVKIGYSSPLTLTDNGSKVKKGCKGDANVFKPTLMCAVPLLLQRIYKGIIDNMRKKGWAMEELFNYFVAYKIKWQXRGFDXPLLNKTLFRTVRYFLGGRVRLLLNGGAPISHETHLFTRTTLSVPVLQAYGLTELSGAAAISNEYDFSTCRVGPPLIGVKVKLVNWEEGGYTINDKPFPRGEIHCGGESLCQGYFKNPEKTADDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILKTCPFIENICTYADPLKDYLFAIAIPDKAHVN---QRGLTQEDACKDPSFISSVTKEIGDYGLKNGLVKFEVPAKILFVSGEWTTDNGLITAAFKTRRRPIVEXYRIQVDK 2519          

HSP 2 Score: 609.372 bits (1570), Expect = 0.000e+0
Identity = 286/530 (53.96%), Postives = 383/530 (72.26%), Query Frame = 0

Query:  259 REIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKK---RSRPQLQCQVIPYDWLLNSMQTGDPSRQP-PLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-------------------NNYSVNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVE 765
            RE+ +  +DTM   F Y   +Y  + CLGTRE+  EE+E+Q NGK + KY++G YK+ T+  +K E   ++ G + L L   + +A+ A+TR EW++ A A F  +  +VTLYTNLG DG++H INETEV ++VC+ + + K+++++ +C  +  VI++  ++ K    +       + I +  L+      D  R P P P+D AIIMYTSGSTGTPKGV++SHKN++S +KA++N+  F+  DRY+ YLPLAHVLEL++E  C++ G+KIGYSSP TLTNKSSKVK GCKGDAN+ +PTLMCSVP+VLERIYKS+VDTM+RQGWA+EELFHYF++YKMKW+ RGFDTPLLNKTLFRK RYF+GGRVRL++SGG P+S +T S++RT +CVPVLQGYGLTE T   T                       V+W++G Y ++DK F RGEIH GGD +S+GY+K PE+  +DFYEEDG+RWF+TGDIG+++ DGVLK++DR KDLVKL  GEYIS GKVESILK C   E
Sbjct: 1363 RELCKVKVDTMFGAFEYSVAKYGXRVCLGTREVFSEEEELQPNGKIFKKYVMGAYKFMTYDELKEESYNLAEGFRSLKLPTKTHIAMFAETRKEWILTAYAAFINNFTLVTLYTNLGEDGVIHGINETEVKLVVCTVDMLPKIKKVIKSCPQIGVVIVMEANTGKTIDLKPYENNNVEFIMFRDLITRKSNED--RYPAPTPRDPAIIMYTSGSTGTPKGVVISHKNIISGVKALMNVVTFKPSDRYMGYLPLAHVLELISEMVCLICGVKIGYSSPLTLTNKSSKVKRGCKGDANVFKPTLMCSVPLVLERIYKSVVDTMRRQGWAIEELFHYFVAYKMKWQXRGFDTPLLNKTLFRKXRYFIGGRVRLLISGGXPISHDTQSVSRTALCVPVLQGYGLTELTGGVTMSDPYDRTTCRVGPPLLGLKLKLVDWEEGNYTINDKSFPRGEIHCGGDSISIGYFKNPEKTAEDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILKTCXYYE 1890          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000007052 (supercontig:LSalAtl2s:LSalAtl2s3:1758018:1760492:-1 gene:EMLSAG00000007052 transcript:EMLSAT00000007052 description:"maker-LSalAtl2s3-snap-gene-17.37")

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 306/712 (42.98%), Postives = 436/712 (61.24%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNR-EIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSR-PQLQCQVIPYDWLLNSMQTGDPSR-QPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFR--AGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT----NNYS---------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKSL---------------QDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            ++++ VL  +  +  ++D+  YPIY V Y PW+ R        R E I     +  +  P K N+  I  +G+ TMD+V+ +  + Y E+  LG+R ++  EDE QS+GK + K  LG Y W+++ +    VD    GL+ +GL    K+A+ A+TRAEW +   A F ++I +VTLYTNLG +G+ H IN+TE   ++ S E + K+  +LP   +V+ VI++   +    S+ P+   ++  ++ L+++     P++ +PP P D AIIMYTSGSTG PKGV+L+H+NL+S +K++ +  E +  + D+YIA+LPLAHVLELL E   ++ G++IGYSSP TL + S+ +K+G  GDA +L+PT+M  VP+VL RIYK I   M  +G     +F +   Y++K    G  TPLLNK LF KI   +GG+V+L++SGGAPLS E H   R C+ V VLQGYGLTETTA AT    N+ S               VNW+DG Y V+++P  RGEI IGGD VS GYYK+PE+ ++DF EE G RWFRTGDIGEV   G +KIIDR KDLVKLQ GEYIS GKVES LK C +V+NIC+  DS K   +A+I+P+K  L  +  + L               + C KD   + MV  E++    +K L R E P  + +    W PE+GLVTAA KI+RR I   Y+  +++
Sbjct:   41 VMVQPVLAILKFVVRLWDFFTYPIYQVIYKPWKKR--NDFGKLRSEIIEKTDSYVLIRSPEKENKLTIGLEGIMTMDKVWKWALQVYGERPILGSRSVLSMEDETQSDGKVFKKLELGSYHWKSYRDCDIIVDNFGRGLRHIGLCPKDKVAVYAETRAEWFLATQAAFSQNISIVTLYTNLGPEGITHGINQTEAKCVISSNELLPKLLNLLPQLPNVEKVIVIPDHTSITNSKVPEGGVEIYKFEELVDAGSNWKPAKSEPPQPDDIAIIMYTSGSTGVPKGVLLTHRNLVSTLKSLNSTFEIKISSNDKYIAFLPLAHVLELLCEMMMVIRGVRIGYSSPNTLLDTSTMIKSGTHGDAAVLQPTVMAGVPLVLTRIYKGISQKMDSRGELFNSIFRFCYDYRLKSIRNGLPTPLLNKILFGKIHKLIGGKVKLIISGGAPLSDEXHDFXRVCLGVDVLQGYGLTETTASATVMDINDISTGTVGPPNQFLDIKLVNWEDGNYKVTEQPRPRGEILIGGDCVSNGYYKMPEKTKEDFLEERGKRWFRTGDIGEVTELGTIKIIDRKKDLVKLQYGEYISLGKVESALKTCPVVDNICIYGDSFKSNCVALIVPNKTVLGKWADQKLGMYSQTEIEHNNNAFERCCKDERVIDMVLREVRLHCSKKKLQRFETPVAVYLCKENWGPETGLVTAAFKIKRRPIQEFYQKDLDQ 750          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000010073 (supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10")

HSP 1 Score: 542.732 bits (1397), Expect = 0.000e+0
Identity = 272/698 (38.97%), Postives = 423/698 (60.60%), Query Frame = 0

Query:  201 LFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHAR---REDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPS-SPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAI-INIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS-------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFY-EEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSF------VRKSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            L  I  + +VYD++ YPIY     PW VR    +  +R   + D    + +  +   T+        G+D M   +      + ++DCLGTR ++EE+DE+Q +GK + K+ +G+Y+W++F+ ++        GL+ELGLE    + I A+++AEWM  A  CF+++  +VTLY NLG D + H +N+T+VS L+ + + + K++ +L     +K +I      +   RS  +   +++ +  ++      +    PP   D AIIMYTSGSTG PKGV+L H++L+  + A  + + +    + Y+ YLPLAH+ ELLAE + +++GIK+GYSSP T+ +KS+KV  G KGD ++LRPTLMC+VP+V++RIYK I + + ++   +  L H+   YK  +   G  TP++N  +FR +R  +GG+VR+++SGGAPLS   H      + +P+ QGYGLTE+ A  +  +                    +NW+DGGY V+DKP  RGEI IGG  ++ GYYKLP++  +DFY +E+ VRWF+TGD+GE   DG LKIIDR KDLVKLQ GEY+S GKVE+++K C LVEN+C   D +K Y +A+++PD+++LT           SL+D   + + +  +  E   +G++ GL + E+P  I +    WTPESGLVTAA K+RR+ I   Y+ +++K
Sbjct:   27 LGVIKTLIYVYDFLTYPIYAAIQRPWEVREASNALRSRPISKSD--SEITYAPVYRSTERLDAFRAAGVDNMLDCWKLAVSLHGDKDCLGTRHVLEEKDEVQPDGKVFKKWAMGEYQWKSFNEIEELSTDFGKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTHDLLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVIELGSKSETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGTIAAFHLVVKDKIPEETYLGYLPLAHIYELLAEMTMLIHGIKVGYSSPNTMIDKSTKVMKGSKGDCSVLRPTLMCAVPLVIDRIYKGIHENLSKKSKFMRSLLHFCYHYKSHYRRLGMRTPIMNALIFRPMRAIVGGKVRVIVSGGAPLSHNIHDYVSCALDIPIHQGYGLTESCAGGSVMHREELSTGRVGPPGQGVLIRLINWEDGGYRVTDKPRPRGEIVIGGPNIAHGYYKLPQKTAEDFYKDENNVRWFKTGDVGEFAEDGTLKIIDRKKDLVKLQYGEYVSLGKVETVIKTCPLVENVCAYGDPTKSYCVALVVPDRNKLTELGESIGIYNASLEDLCMNEKVIATMQKETASYGRKNGLEKFEVPGAITLCTEMWTPESGLVTAAFKLRRKPIQDFYQKELKK 722          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000007051 (supercontig:LSalAtl2s:LSalAtl2s3:1688664:1690882:-1 gene:EMLSAG00000007051 transcript:EMLSAT00000007051 description:"augustus_masked-LSalAtl2s3-processed-gene-17.11")

HSP 1 Score: 498.819 bits (1283), Expect = 4.302e-166
Identity = 279/698 (39.97%), Postives = 414/698 (59.31%), Query Frame = 0

Query:  196 LLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRR-YKKSTHARREDIPGGVIFHSLTEPTKVNR-EIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILL-NPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSR-QPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFR--AGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYSVNWDDG-----GYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKSL---------------QDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +++ VL  +  +  ++D+  YPIY V Y PW+ R  + K      E     V+  S   P K N+  I  +G+ TMD+V+ +  + Y E+  LG+R ++  EDE QSNGK + K  LG Y W++F +    V+    GL+ +GL    K+A+ A+TRA+ ++     F ++I +VTLYTNLG +G+ H IN+TE   ++ S E + K+  +LP   +V  VI++ + +S      P+++ ++  +D L+++     P + +PP P D AI+MYTSGSTG PKGV+L+H+NL++ + +I +  + +  + D+YIA+LPLAHVLELL E   ++ GI+IGYSSP TL + S+ +K+G  GDA +L+PTLM  VP+VL RIY  I   M  +G   + +F +F  Y++K    G  TP+LNK LF K    +GG+V+ + +GGAPLS E H   R C+ V V+QGYGLTETTACAT   S +   G        +  K  +  ++          YYK+ E+ ++DF+EE G RWFRTGDIGEV   G +KIIDR KDLVKLQ GEYIS GKVES L  C +V+NIC+  DS K   +A+I+P+K  L  +  + L                 C +D   + MV  E++    +  L R E+P  + +    W+PE+GLVTAA KI+RR I   Y+  +++
Sbjct:    1 MVRIVLGILKFVVRLWDFFTYPIYQVIYKPWKKRNNFGKLRSEIIEKTDSHVLIRS---PEKENKLTIGLEGIITMDKVWKWALQVYGERPILGSRSVLSVEDETQSNGKVFKKLELGSYHWKSFRDCDIIVENFGRGLRHIGLCPKDKVAVYAETRAQGLLATLGAFSQNISIVTLYTNLGPEGITHGINQTEAKCVISSNELLPKLLNLLPQLPNVNKVIVIPDHTSISNSQVPEVEVKIYMFDELVDAGSNWKPVKSEPPQPDDIAIVMYTSGSTGVPKGVLLTHRNLVTTLISINSTIDIKTSSNDKYIAFLPLAHVLELLCEMMMVIRGIRIGYSSPNTLLDTSTMIKSGTHGDATVLQPTLMTCVPLVLTRIYNGINQKMDTRGKLFKSIFRFFYDYRLKSIRNGLPTPVLNKILFGKFHKLIGGKVKFIFAGGAPLSDEVHDFIRVCLGVDVIQGYGLTETTACATLMDSSDISTGTAGAPNQCIDIKLVNWEDVFCKFFFFCYSYYKIHEKTKEDFFEEKGKRWFRTGDIGEVTELGTIKIIDRKKDLVKLQYGEYISLGKVESALTTCPVVDNICIYGDSFKSNCVALIVPNKTVLGKWADQKLGMYSQTEIEHNSDTFSKCCQDERVIDMVLEEVRLHCLKNKLQRFEIPVAVYLCKENWSPETGLVTAAFKIKRRPIQEFYQKDLDQ 695          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000009272 (supercontig:LSalAtl2s:LSalAtl2s591:175091:177076:1 gene:EMLSAG00000009272 transcript:EMLSAT00000009272 description:"augustus_masked-LSalAtl2s591-processed-gene-1.0")

HSP 1 Score: 467.233 bits (1201), Expect = 1.927e-154
Identity = 244/641 (38.07%), Postives = 376/641 (58.66%), Query Frame = 0

Query:  273 FNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQ-----LQCQVIPYDWLLNSMQTGDPSRQPPL---------PQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAI----INIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTET----TACATNNYS---------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVR---------KSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
             N+    +  + CLGTR I+  +    ++G+   K+ L DYKW +++++  ++ + + GL+ LG +   K+ I A+T A+W+I A  CF+ +  +VT+YTNLG DG+ +++N+T+  V++ SE+ + K++ +L   GH+ ++I +     K     Q      Q   +        ++ G  S  P L         P+D AIIMYTSGSTG PKG +LSH N+LS + A      N+      DRYIAYLPLAH+ E   E + ++ G  IGYS P TLT+    +K G  GDA  L+PT M +VP++L+RI+K I   + ++G   E LF +   Y+  W   GFDTP++NK +F+K+R  LGG ++ ++ GGAPLS E H    T +CV + QGYGLTE+    T C  ++Y                VNW++GGY V++    RGEIH+GG++V++GYY + E+ ++ FYE+DG+RWF TGDIG++Q  G  KIIDR KDLVKLQ GEY+S GKVE   K    V+ IC   +S K  ++A+++PDK +  + ++         +       DPE + +    +Q +  +K L + E+P++ ++    WTP+ GLVTAALK++RR I  +Y+  I++
Sbjct:    1 MNHAINVHGSKQCLGTRPILSIDTVQNNDGRLLTKHELADYKWLSYNDVNEQIQSFAHGLRYLGFQPREKIVIYAETSAKWLISALGCFRNAYTLVTVYTNLGEDGVAYSLNQTDAKVVITSEDLLPKLKGILKKGGHIHTIIYIEKPLEKMNIHAQNDDGSEQASEVNVYSFTEVIRIGVSSYSPDLDCYESWKATPEDIAIIMYTSGSTGNPKGALLSHANILSGILACCPYGXNLLVPPEEDRYIAYLPLAHIFEFTLEMTMILRGTSIGYSGPNTLTDAGXMIKRGHMGDARALKPTSMIAVPVILDRIFKGIQFKVSQEGAFKENLFTFCYQYRSMWRKLGFDTPIMNKLIFKKVRESLGGEIQSIIGGGAPLSPEVHDFLETVLCVKISQGYGLTESCGGITLCHYDDYHVGEVGYPLPGAYIKLVNWEEGGYKVNNSTAPRGEIHVGGNVVAMGYYNMSEKTKESFYEKDGIRWFPTGDIGQLQPTGAFKIIDRKKDLVKLQMGEYVSLGKVEGAWKIHPFVDAICSYGNSFKFDIVAVVVPDKYKFLNHLKNNHAEEDLNRDFSTICSDPEIVIIFLKTLQVYSAKK-LEKFEIPKRXLLEPEPWTPDCGLVTAALKLKRRTIQEKYQEDIDR 640          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000004934 (supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9")

HSP 1 Score: 446.432 bits (1147), Expect = 1.494e-144
Identity = 247/696 (35.49%), Postives = 390/696 (56.03%), Query Frame = 0

Query:  203 FIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPA-CGHVKSVILLNPSS-PKKRSRPQLQCQVIPYDWLLNS-----MQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINI--AEFRAGDR--YIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT----NNYSV---------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKSL---QDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQI 865
             I LI   +D +  PI  + Y PW+    K ST          +++ SL      +R   E G DT+  + +Y  K  + ++C GTR I   E  +     T  KY  G+Y+W T+ ++       S GL+ L      K+ + A++ A+W++ A  CF+ S+ VVT+YTNLG++G+ H I +T+  V++ S+  + ++Q++L +  G ++++I L        ++ P+    V  ++ +L        Q  +     P  +D A+IMYTSGS+G PKGV+LSH N++S +  I++     F   D+  YI YLPLAH+ EL  E +    GI IG+S P TL N  S VK G  GD   L+PT M  VP++ +RIYK I+  +   G   E LF++   YK KW   G +TP++N   F+KI+ FLGG V  ++SGGAPLS++ H        + + QGYGLTET          ++S+               NWD+G Y V DKP+ RGEIH+GG ++++GY++L +  E++FYEE+GVRWF+TGDIG++Q  G LKIIDR KD++KLQ GEY+S GKVE+ L    LV+ IC+  +  ++ ++ +I+P++  +   +++SL    +   D   L+    +         L + E+P+ + +  G WT E+GL+T + K++R+ I  +Y+ +I
Sbjct:  102 LIKLIVLTWDLLVSPIINLIYQPWKKGNQKLSTIQTLLQNDSTIVYRSLDNNFNEDRRKIEYG-DTLVDLIDYASKANMSKNCFGTRRIFNVEQIVTKENNTMTKYDQGEYEWLTYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQNLLKRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAVIMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNFVRLDKESYIGYLPLAHIFELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKWRKLGMNTPIINLLFFKKIQQFLGGNVSTIVSGGAPLSAQAHEFMEIVFDLKLSQGYGLTETCGGFILGRKEDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYLGTHPLVDIICVYGEPERNCIVGLIIPNEKSI-KILKESLGLRNESVYDNNLLRSQVRQALANHSEAKLLKFEIPKAVWLIHGPWTAENGLITTSYKLKRKAIQNKYQEKI 795          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000000839 (supercontig:LSalAtl2s:LSalAtl2s1141:106424:108541:-1 gene:EMLSAG00000000839 transcript:EMLSAT00000000839 description:"maker-LSalAtl2s1141-augustus-gene-0.8")

HSP 1 Score: 102.834 bits (255), Expect = 7.536e-23
Identity = 138/607 (22.73%), Postives = 250/607 (41.19%), Query Frame = 0

Query:  311 GDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVL-VCSEESMAKVQEMLPACGHVKSVI--LLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDP--------SRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEF----RAGDRYIAYLPLAHVLELLAETSC-MMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQG--------WAVEELFHYFMSY-KMKWEDRGFDTPLLNKTLFRKIRYFLG-GRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYSVNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIIL------------PDK---------DQLTSFVRKSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKI---IIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            GD    TF +   +V   +  + + GLE G+ + IL     EW I          +   +Y     D   +  +    +++ V ++E + K+Q +     H++ +I  +  P+ P           V+ ++  + S+ T +P        S        C  ++YTSG+TG PKGVMLSH N+    K   N  E        +R+++YLPL+H+  ++ +    + + + + ++    L    ++    C        PTL   VP V E++ + I+   K           WA +  + Y  S      +D+     + NK +F+K++  LG  +    +S GAPLS ET     + + + +++ YG++E       N    W  G               +G D        LP    K   ++DG    +    GE+   G L++  R K+L+    GE I    +E  LK    + +  +V   +K YL   +              D+         ++L S VR ++ +  ++PE  + V   IQ+   +  +N T   QKI   II   +++  +G +   LK++R  I+ +Y   ++K
Sbjct:   62 GDLITWTFRDYLKDVRTAAKAMLKCGLEEGNGVCILGFNSPEWFIADLGAIYAGGIPSGIYPTNNPDSCKYIASHCHCTIMFVENQEQLQKIQSIKTQLPHLRKIIQYIGEPTDP----------DVLSWNAFI-SLGT-EPELEXLLEISLSKIXANKCCHLVYTSGTTGNPKGVMLSHDNITFTAK---NTGEHYKLKEKEERFVSYLPLSHIAAVIVDIFIPITFKVTVYFADKNALKGTLTETLREC-------HPTLFFGVPRVYEKMQEKIMGASKSTKGLKKHIGRWAKKTGYRYNKSLITHPNQDKDIQYKVANKVVFQKVKALLGFDKCVHFVSSGAPLSIETLEYFMS-LDILIMELYGMSECCGPHXANTLRAWKPG--------------KVGRD--------LPGFLTK--IDKDGEIHMK----GELDSSGFLRVTGRRKELIITAGGENIPPVLIEDKLKASLPILSNAMVIGDNKKYLSCFLTLKVNEDSETHEPSDRLSPLSEEWCNKLGSHVR-TVSEILENPE--EQVLAAIQEGINKYNINATSNAQKIQKWIIIPKDFSVSTGELGPTLKMKRYFILKKYEALVKK 614          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 96.2857 bits (238), Expect = 1.895e-20
Identity = 106/424 (25.00%), Postives = 181/424 (42.69%), Query Frame = 0

Query:  459 CAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDR-YIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLL----NKTLFRKIRYFLG-GRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTET-TACATNNYSVNWDDGGYFV----------SDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCF-LVENICLVADSSKDYLIAIILPDK--------DQLTSFVRKSLQDCA------------KDPECLKMVALEIQDFGKRKGLNRTE------LPQKIIIADGE 838
            C  ++YTSG+TG PKGVMLSH  +    KAI+   ++       ++YLPL+H+     +    +Y             +K + +K         +RPT+   VP V E+  + I +  K Q  +  ++   F    M + + G D  ++    +K +F+K++  LG  R    +SGGAPLS  T     + + +PV + YGL+E    C+           G  +          SD     GEI I G  V +GY    E+   + ++++G  +F TG               R+KDL+    GE ++   +E+ +K+   +V N  +V D+ K   + + +  K         +LT  V+K  ++              K P  +K +   I +  KR   N  +      LP+++ +  GE
Sbjct:  218 CCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALY-----LKGEVYFADKYA-LKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENG-DQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLS-LDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDIG-GEICIKGRCVMMGYL-FNEQATSEAFDKEG--YFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNLGCNYTTVGEILYHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGE 614          

HSP 2 Score: 72.0182 bits (175), Expect = 5.335e-13
Identity = 87/355 (24.51%), Postives = 146/355 (41.13%), Query Frame = 0

Query:  311 GDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEES-MAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQ---PPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTM----KRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLG-GRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTE 656
            GD+   T+S    E+   +    +LGL+    ++I +    EWMI              LY +       + +N +   +LV      + KVQ +L    H+  V+++            L   +I     L   +  D  +Q         C  I+YTSG+TG PKGVM+SH  L    K ++    +      ++YLPL+H+    A  +  +Y + I         +KS+ +K         +RPT+   VP V E+  + I + +    K Q +    L      Y     ++ F   L N+ L++KI+  LG  + +    GGAPL   T     + + + +   YGL+E
Sbjct:  688 GDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIADKLSILWRDLIE----LGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHI----AANALDIY-VVICCRGELYFADKSA-LKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTT-GNKKFIKNLGNQMLYKKIKDSLGLDQCQEFSCGGAPLMPTTTDFFLS-LDIVIKVAYGLSE 1030          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000006219 (supercontig:LSalAtl2s:LSalAtl2s33:201552:203709:-1 gene:EMLSAG00000006219 transcript:EMLSAT00000006219 description:"maker-LSalAtl2s33-augustus-gene-2.8")

HSP 1 Score: 88.1965 bits (217), Expect = 1.501e-18
Identity = 93/379 (24.54%), Postives = 157/379 (41.42%), Query Frame = 0

Query:  314 KWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMI-LANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSE-ESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDW-----LLNSMQTGDPSRQPPLPQ----DCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQG------------WAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLG-GRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYSVN 668
            KW T+     E    + G  ELGLE    + IL     EW + L  A F          TN   + + +  N+  V VLV  + + + KV  +      +K++I+          + + +     Y W     L  + Q    + Q  L       C  ++YTSG+TG PKGVM+SH N+    +   +    + GDR ++YLPL+H     +    M   + I         ++++ +K         +RP     VP    R+Y+ I + M+  G            WA +   ++ M      +  G   PL  K +F+K++  LG  + R+   G AP+S +T     + + +P+   YG++E++   T N   N
Sbjct:   63 KW-TYKEYYEEAQLAAEGFIELGLEPFHSVGILGFNAPEWSLSLVGAIFAGGFGAGIYATNSA-EAVKYVANDCRVQVLVVEDSKQLQKVNAIKEQLTFLKAIIVYG-------YQEKFEGGDNIYSWREFMDLARNAQKKGENVQNRLENISINQCCSLIYTSGTTGPPKGVMMSHDNVTWTARTSNHYLGLKHGDRGVSYLPLSH-----SAAQMMDIWMPIAQGMSMYFCDRNA-LKGSLVSTLREVRPAYFFGVP----RVYEKIEEKMRELGKANTGFKKAFGEWAKKTGLNHNMKSLEGQQPGGISYPLAKKLIFKKVKEGLGLDKARMCGIGAAPVSRKTLEYFLS-LDIPLYDCYGMSESSGPQTGNRPGN 421          

BLAST of GK21733 vs. L. salmonis genes
Match: EMLSAG00000010718 (supercontig:LSalAtl2s:LSalAtl2s70:458722:474012:1 gene:EMLSAG00000010718 transcript:EMLSAT00000010718 description:"augustus_masked-LSalAtl2s70-processed-gene-4.2")

HSP 1 Score: 69.707 bits (169), Expect = 1.633e-12
Identity = 103/425 (24.24%), Postives = 180/425 (42.35%), Query Frame = 0

Query:  286 LGTREIIEEEDEIQSNGKTY---VKY-ILGDYKWRTFSNMKY--EVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAK----VQEMLPACGHVKSVILLN---PSSPKKRSRPQLQCQVIPYDWL------LNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDR--------YIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANI-----LRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELF-----------HYFMSYKMKWEDR--GFDTPLLNKTLFRKIRYFLGGRVR-LMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNN 664
            LG+ E I     +Q+  + Y   V + I  D +W +++  +Y  EV+ V+      GL R   +AIL +   EW++   A      + V L   L  D +     + +  V+V   E+  K    +Q  LP    ++++I ++   P S K+R     +  ++ ++ +      L   +  D  ++  + Q C II YTSG+  TPKGVM+SH NL    K  + +      +R         I+ LPL+ +       S  +  I        TL   S+K     +GDA       ++PT++ +  ++ ERI+  + + +KR    ++++            H     K+K   +   + T L   T+ +K +  LG   R +  S GAP++ E          V V + +G TE     T N
Sbjct:   68 LGSEEPISVPTLLQNIAENYPDVVAFKIKRDEEWISWTYEQYLKEVNTVAKSFIXXGLHRHXSVAILGNNSPEWIVSDVAAIFSGGIAVGLNKWLSPDEIAKICIDCKADVIVVQNEAFLKKILLIQHKLP---ELRTIIQIDGDPPLSDKRRLHRTHKKIILSWEEMIVLGHNLTDDRLRDRLKKVAINQVCTII-YTSGANETPKGVMISHDNLTWCAKTSMGLIRAPGFNRMPAPGEEILISVLPLSSI-------SAQIIDIYYTLCVVGTLCISSNK-NLLTQGDAFFDTVFDVQPTVLFAPTLIYERIFHKLRN-LKRSMSGIQKIVLDWSSCTLRDKHLNEQKKIKATRKLNQWQTALAKNTVTKKYKEMLGFTSRTVFFSRGAPMAREVLRFLSGYDIV-VHETFGQTENCGLLTAN 478          

BLAST of GK21733 vs. SwissProt
Match: gi|13432172|sp|O60488.2|ACSL4_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase 4; AltName: Full=Long-chain acyl-CoA synthetase 4; Short=LACS 4)

HSP 1 Score: 523.857 bits (1348), Expect = 2.571e-174
Identity = 288/715 (40.28%), Postives = 422/715 (59.02%), Query Frame = 0

Query:  193 KYILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGD------------PSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT----NNYSV---------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDF-YEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSF-----VRKSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            K  +L  +L  ++L+  +Y  + + P YF+     +    K+       D PG   + S+T    +   I+  G DT+D++F++   ++ ++D LGTREI+ EE+E+Q NGK + K ILG+YKW  +  +   V+    GL  LGL+  + +AI  +TRAEWMI A  CF+ +  +VTLY  LG + +VH +NE+E S L+ S E + +K++  L     VK +I ++  +  K   P+         + ++SMQ+ +            PSR  P P D AI+MYTSGSTG PKGVM+ H NL++ M      I      D YI YLPLAHVLEL AE SC  YG +IGYSSP TL+++SSK+K G KGD  +L+PTLM +VP +++RIYK+++  ++   +  + LF     YK++   +G+D PL N  LF+K++  LGG VR+MLSGGAPLS +TH     C C P+ QGYGLTE+    T     +Y+                +W +GGY ++DKP  RGEI IGG  +S+GY+K  E+  +D+  +E+G RWF TGDIGE   DG L+IIDR KDLVKLQ GEY+S GKVE+ LKNC L++NIC  A S + Y+I+ ++P++ +LT       V  +  D   +P     +  EI++      L R E+P K+ ++   WTPE+GLVT A K++R+++   Y   IE+
Sbjct:    4 KLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSP-YRSVTHFDSL-AVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPE--------GFEIHSMQSVEELGSNPENLGIPPSR--PTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIER 706          

BLAST of GK21733 vs. SwissProt
Match: gi|341940183|sp|Q9QUJ7.2|ACSL4_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase 4; AltName: Full=Long-chain acyl-CoA synthetase 4; Short=LACS 4; Short=mACS4)

HSP 1 Score: 522.702 bits (1345), Expect = 8.765e-174
Identity = 289/715 (40.42%), Postives = 420/715 (58.74%), Query Frame = 0

Query:  193 KYILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGD------------PSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT----NNYSV---------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDF-YEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSF-----VRKSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            K  +L  +L  ++L+  +Y  + + P YF+     +    K+       D PG   + S+T    +   I+  G DT+D++F++   ++ ++D LGTREI+ EE+E+Q NGK + K ILG+YKW  +  +   V+    GL  LGL+  + +AI  +TRAEWMI A  CF+ +  +VTLY  LG + +VH +NE+E S L+ S E + +K++  L     VK +I ++  +  +   P+           ++SMQ+ +            PSR  P P D AI+MYTSGSTG PKGVM+ H NL++ M      I      D YI YLPLAHVLEL AE SC  YG +IGYSSP TL+++SSK+K G KGD  +L+PTLM +VP +++RIYK+++  ++   +  + LF     YK++   +G+D PL N  LF+K++  LGG VR+MLSGGAPLS +TH     C C P+ QGYGLTE+    T     +Y+                +W +GGY V DKP  RGEI IGG  +S+GY+K  E+  +D+  +E+G RWF TGDIGE   DG L+IIDR KDLVKLQ GEY+S GKVE+ LKNC L++NIC  A S + Y+I+ ++P++ +LT       V  S  D   +P     +  EI++      L R E+P K+ ++   WTPE+GLVT A K++R+++   Y   IE+
Sbjct:    4 KLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSP-YRSVTHFDSL-AVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTINRAEYPE--------GLEIHSMQSVEELGAKPENLSVPPSR--PTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIER 706          

BLAST of GK21733 vs. SwissProt
Match: gi|108935948|sp|Q5R668.2|ACSL3_PONAB (RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3)

HSP 1 Score: 520.005 bits (1338), Expect = 1.251e-172
Identity = 288/729 (39.51%), Postives = 421/729 (57.75%), Query Frame = 0

Query:  184 HIGSKSASAK--YILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEE---------QGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQ--LQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-------NNYSV------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYE-EDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKS---------LQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            H+ SK ++ K  + +   +L+FI+ +  +Y  + Y P YF     +   R +KS   + + +         ++P    R +            G DT+D+VF Y   ++  +  LGTRE++ EEDE+Q NGK + K ILG Y W ++ ++         GL+ LG +  + +AI  +TRAEWMI A ACF  +  +VTLY  LG   +VHA+NETEV+ ++ S+E +  K+++++     ++ +I ++   P     P+  +   +   + L       +     PLP D A+IMYTSGSTG PKGVM+SH N+++ +  +   I E    D YI YLPLAHVLEL AE  C+ +G +IGYSSP TL ++SSK+K G KGD ++L+PTLM +VP +++RIYK++++ +         LF    +YKM+   +G +TPL N  +FRK+R  LGG +RL+L GGAPLS+ T      C C PV QGYGLTE+    T       N   V            NW++GGYF +DKP  RGEI IGG IV++GYYK   + + DF+E E+G RW  TGDIGE + DG LKIIDR KDLVKLQ GEY+S GKVE+ LKN  LV+NIC  A+S   Y+I  ++P++ +LT   RK             C  + E LK+++    +      L + E+P KI ++   WTPE+GLVT A K++R+++   Y+  IE+
Sbjct:    4 HVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYF-----FSESRQEKSNRIKAKPVN--------SKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCNSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQIVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLS----EAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIER 715          

BLAST of GK21733 vs. SwissProt
Match: gi|6016483|sp|O35547.1|ACSL4_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase 4; AltName: Full=Long-chain acyl-CoA synthetase 4; Short=LACS 4)

HSP 1 Score: 517.694 bits (1332), Expect = 1.872e-172
Identity = 275/646 (42.57%), Postives = 392/646 (60.68%), Query Frame = 0

Query:  261 IEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGD------------PSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT----NNYSV---------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDF-YEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSF-----VRKSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            I+  G DT+D++F++   ++ ++D LGTREI+ EE+E+Q NGK + K ILG+YKW  +  +   V+    GL  LGL+  + +AI  +TRAEWMI A  CF+ +  +VTLY  LG + +VH +NE+E S L+ S E + +K++  L     VK +I ++  +  +   P+           ++SMQ+ +            PSR  P P D AI+MYTSGSTG PKGVM+ H NL++ M      I      D YI YLPLAHVLEL AE SC  YG +IGYSSP TL+++SSK+K G KGD  +L+PTLM +VP +++RIYK+++  ++   +  + LF     YK++   +G+D PL N  LF+K++  LGG VR+MLSGGAPLS +TH     C C P+ QGYGLTE+    T     +Y+                +W +GGY V DKP  RGEI IGG  +S+GY+K  E+  +D+  +E+G RWF TGDIGE   DG L+IIDR KDLVKLQ GEY+S GKVE+ LKNC L++NIC  A S + Y+I+ ++P++ +LT       V  S  D   +P     +  EI++      L R E+P K+ ++   WTPE+GLVT A K++R+++   Y   IE+
Sbjct:   30 IDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPE--------GLEIHSMQSVEELGSKPENSSIPPSR--PTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIER 665          

BLAST of GK21733 vs. SwissProt
Match: gi|115502350|sp|O95573.3|ACSL3_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3)

HSP 1 Score: 514.998 bits (1325), Expect = 9.123e-171
Identity = 286/729 (39.23%), Postives = 420/729 (57.61%), Query Frame = 0

Query:  184 HIGSKSASAK--YILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEE---------QGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQ--LQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-------NNYSV------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYE-EDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKS---------LQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            H+ SK ++ K  + +   +L+FI+ +  +Y  + Y P YF     +   R +KS   + + +         ++P    R +            G DT+D+VF Y   ++  +  LGTRE++ EEDE+Q NGK + K ILG Y W ++ ++         GL+ LG +  + +AI  +TRAEWMI A ACF  +  +VTLY  LG   +VHA+NETEV+ ++ S+E +  K+++++     ++ +I ++   P     P+  +   +   + L       +     PLP D A+IMYTSGSTG PKGVM+SH N+++ +  +   I E    D YI YLPLAHVLEL AE  C+ +G +IGYSSP TL ++SSK+K G KGD ++L+PTLM +VP +++RIYK++++ +         LF    +YKM+   +G +TPL +  +FRK+R  LGG +RL+L GGAPLS+ T      C C PV QGYGLTE+    T       N   V            NW++GGYF +DKP  RGEI IGG  V++GYYK   + + DF+E E+G RW  TGDIGE + DG LKIIDR KDLVKLQ GEY+S GKVE+ LKN  LV+NIC  A+S   Y+I  ++P++ +LT   RK             C  + E LK+++    +      L + E+P KI ++   WTPE+GLVT A K++R+++   Y+  IE+
Sbjct:    4 HVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYF-----FSESRQEKSNRIKAKPVN--------SKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLS----EAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIER 715          

BLAST of GK21733 vs. SwissProt
Match: gi|13431600|sp|Q63151.1|ACSL3_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Brain acyl-CoA synthetase II; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3)

HSP 1 Score: 504.212 bits (1297), Expect = 1.523e-166
Identity = 280/725 (38.62%), Postives = 413/725 (56.97%), Query Frame = 0

Query:  184 HIGSKSASAKY--ILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQ---------GLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQ--LQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-------NNYSV------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYE-EDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK-----SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            H+ S  ++ K    +   +L+FI+ I  +Y  + Y P YF+        + +K  H + + +         ++P    R +            G DT+D+VF Y   ++ ++  LGTREI+ EEDEIQ NGK + K ILG Y W ++ ++         GL+ LG +  + +AI  +TRAEWMI A ACF  +  +VTLY  LG   +VH +NETEV+ ++ S+E +  K+++++     ++ +I ++   P     P+  +   +     L         +   PLP D A+IMYTSGSTG PKGVM+SH N+++++  +   I      D YI YLPLAHVLEL AE  C+ +G +IGYSSP TL ++SSK+K G KGD ++L+PTLM +VP +++RIYK++++ +         LF    +YKM+   +G  TPL ++ +FR +R  LGG +R++L GGAPLS+ T      C C PV QGYGLTE+T   T       N   V            NW++GGYF +DKP  RGEI IGG  V++GYYK   + + DF+E E+G RW  TGDIGE   DG LKIIDR KDLVKLQ GEY+S GKVE+ LKN  L++NIC  A+S   Y+I  ++P++ +LT   R      + ++     E    V   + +      L + E+P KI ++   WTPE+GLVT A K++R+++   Y+  IE+
Sbjct:    4 HVSSTPSTMKLKQTIHPILLYFIHFIISLYTILTYIPFYFLCE-----SKQEKPNHIKAKPVS--------SKPDSAYRSVNSMDGLASVLYPGCDTLDKVFMYAKNKFKDKRLLGTREILNEEDEIQPNGKVFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQKPKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAAVQALGVKADVDKKAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQISKGCSTPLCDRFVFRNVRRLLGGNIRVLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVPNQKELTELARTKGFNGTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIER 715          

BLAST of GK21733 vs. SwissProt
Match: gi|341940182|sp|Q9CZW4.2|ACSL3_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3)

HSP 1 Score: 501.516 bits (1290), Expect = 2.003e-165
Identity = 281/725 (38.76%), Postives = 411/725 (56.69%), Query Frame = 0

Query:  184 HIGSKSASAKY--ILLKAVLFFIYLINHVYDYICY-PIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEE---------QGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESM-AKVQEMLPACGHVKSVILLNPSSPKKRSRPQ--LQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-------NNYSV------------NWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYE-EDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK-----SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            H+ S  ++ K    +   +L+FI+ I  +Y  + Y P YF+        + +K    + + +         ++P    R I            G DT+D+VF Y   ++  +  LGTREI+ EEDEIQ NGK + K ILG Y W ++ ++         GL+ LG +  + +AI  +TRAEWMI A ACF  +  +VTLY  LG   +VH +NETEV+ ++ S+E +  K+++++     ++ +I ++   P     P+  +   +     L         +   PLP D A+IMYTSGSTG PKGVM+SH N+++++  +   I      D YI YLPLAHVLEL AE  C+ +G +IGYSSP TL ++SSK+K G KGD ++L+PTLM +VP +++RIYK++++ +         LF    +YKM+   +G  TPL ++ +FR +R  LGG +RL+L GGAPLS+ T      C C PV QGYGLTE+T   T       N   V            NW++GGYF +DKP  RGEI IGG  V++GYYK   + + DF+E E+G RW  TGDIGE   DG LKIIDR KDLVKLQ GEY+S GKVE+ LKN  L++NIC  A+S   Y+I  ++P++ +LT   R      + ++     E    V   + +      L + E+P KI ++   WTPE+GLVT A K++R+++   Y+  IE+
Sbjct:    4 HVSSTPSTMKLKQTINPILLYFIHFIISLYTILTYIPFYFLCE-----SKQEKPNQIKAKPVS--------SKPDSAYRSINSVDGLASVLYPGCDTLDKVFMYAKNKFKNKRLLGTREILNEEDEIQPNGKIFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQKPKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAAVQALGVKANVEKKAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQISKGCSTPLCDRFVFRNVRRLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKTDFFEDENGQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVPNQKELTELARTKGFKGTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIER 715          

BLAST of GK21733 vs. SwissProt
Match: gi|75169926|sp|Q9CAP8.1|LACS9_ARATH (RecName: Full=Long chain acyl-CoA synthetase 9, chloroplastic)

HSP 1 Score: 445.662 bits (1145), Expect = 2.488e-144
Identity = 253/655 (38.63%), Postives = 370/655 (56.49%), Query Frame = 0

Query:  249 HSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIA-EFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMK------WEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS---------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDF-YEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKSLQD-------CAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            H  TEP   + E     + T+ ++F   C  + ++  LGTR++I  E E   +GKT+ K  LGDY+W TF      V   + GL ++G +   ++AI ADTR EW I    CF+R++ VVT+Y++LG + L H++NETEV+ ++C  + + K+ ++      VK VI ++   P   +   +          L      DP+   PL  D A+IMYTSGSTG PKGVM++H N+L+ + A++ I  +    D Y+AYLPLAH+LEL AE+     G  IGY SP TLT+ S+K+K G KGD   L+PT+M +VP +L+R+   +   +  +G   ++LF +  + ++       +   G +  L +  +FRKIR  LGG++R +LSGGAPLS +T      C+  P+ QGYGLTET  CA   +S                     V+W +GGY  SDKP  RGEI IGG  ++LGY+K  E+ ++ +  +E G+RWF TGDIG    DG L+IIDR KD+VKLQ GEY+S GKVE+ L     VENI + ADS   Y +A+++  +  +  +  K   D       C K+ + +K V   +    K+  L + E+P KI +    WTPESGLVTAALK++R  I  E+   + K
Sbjct:   44 HRFTEPVSSHWE----HISTLPELFEISCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGSKELKKLMDISQQLETVKRVICMDDEFPSDVNSNWMATSFTDVQ-KLGRENPVDPNF--PLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLGKRDIYMAYLPLAHILELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTALKPTIMTAVPAILDRVRDGVRKKVDAKGGLSKKLFDFAYARRLSAINGSWFGAWGLEKLLWDVLVFRKIRAVLGGQIRYLLSGGAPLSGDTQRFINICVGAPIGQGYGLTET--CAGGTFSEFEDTSVGRVGAPLPCSFVKLVDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKEVYKVDEKGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVENIMVHADSFYSYCVALVVASQHTVEGWASKQGIDFANFEELCTKE-QAVKEVYASLVKAAKQSRLEKFEIPAKIKLLASPWTPESGLVTAALKLKRDVIRREFSEDLTK 688          

BLAST of GK21733 vs. SwissProt
Match: gi|75206264|sp|Q9SJD4.1|LACS8_ARATH (RecName: Full=Long chain acyl-CoA synthetase 8)

HSP 1 Score: 439.884 bits (1130), Expect = 1.120e-141
Identity = 243/642 (37.85%), Postives = 364/642 (56.70%), Query Frame = 0

Query:  264 QGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSR-QPPLP--QDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIA-EFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDR------GFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS---------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDF-YEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRKS------LQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +G  TM  +F   CK+Y +   LGTRE I++E    S+G+ + K  LG+YKW+++  +   V   + GL  +G     ++AI +DTRAEW I    CF++SI VVT+Y +LG + L++++NET VS L+C  + + K+  +  +   VK++I +        S        I    +    + G  +  QP LP     A+IM+TSGSTG PKGVM++H NL++    ++ +  +    D YIAYLPLAHV EL AE      G  IGY S  TLT+ S+KVK G KGD + L+PT+M +VP +L+R+ + ++  ++ +G   + LF +    ++   D       G +  L +  +F+KIR  LGG +R ML GGAPLS ++      C+  P+ QGYGLTET  CA   +S                     V+W++GGY +SDKP  RGEI +GG+ V+ GY+   E+ ++ +  +E G RWF TGDIG    DG L++IDR KD+VKLQ GEY+S GKVE+ L +   V+NI + AD    Y +A+++P +  L  +  ++        +  +  E +K V   +   GK   L + ELP KI +    WTPESGLVTAALKI+R QI  ++++++ K
Sbjct:   78 EGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGEYKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYASLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVFTSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKPTIMTAVPAILDRVREGVLKKVEEKGGMAKTLFDFAYKRRLAAVDGSWFGAWGLEKMLWDALVFKKIRAVLGGHIRFMLVGGAPLSPDSQRFINICMGSPIGQGYGLTET--CAGATFSEWDDPAVGRVGPPLPCGYVKLVSWEEGGYRISDKPMPRGEIVVGGNSVTAGYFNNQEKTDEVYKVDEKGTRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGEYVSLGKVEAALGSSNYVDNIMVHADPINSYCVALVVPSRGALEKWAEEAGVKHSEFAELCEKGEAVKEVQQSLTKAGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQIKSKFKDELSK 717          

BLAST of GK21733 vs. SwissProt
Match: gi|74638740|sp|Q9P7D7.1|LCF2_SCHPO (RecName: Full=Long-chain-fatty-acid--CoA ligase 2; AltName: Full=Fatty acid activator 2; AltName: Full=Long-chain acyl-CoA synthetase 2)

HSP 1 Score: 320.087 bits (819), Expect = 1.678e-96
Identity = 210/658 (31.91%), Postives = 332/658 (50.46%), Query Frame = 0

Query:  263 EQGLDTMDQVFNYICKQYLEQDCLGTREIIEE-----------EDEIQSNGKTYVKYILGDYKWRTFSNMKYEVD-AVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPK-----KRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINI--AEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGF-DTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYSVNWD---------------------DGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            E G+ T+  +     K + E+  +G+R++I+E           ++E     K +  Y LG Y++ +F N  YE+  A+  GL   G+   + +   A T A+W   A  C  ++I +VT Y  LG DG+  +++E +   +      + K+   L     VK ++  +  S       K + P ++  +I YD LL+  +       PP   D    MYTSGSTG PKGV+L H+N+++A+  I  I        D  +AYLPLAH+ E + E  C+ +G  +GY+SP TLT+ S      CKGD    RPT++  VP V E I K I+  +       +++F   +S K    +R    +  L+  +F K++   GGR+R  +SGGA L++ T +   +CIC PVL GYGLTET A +       W                      D GY+    P  RGE+ I G  V  GY   PE N+  F  EDG  WF+TGD+GE+     L++IDR K++VK   GEYI+  K+E+      LV N+C  AD +    + I+ PD++ L +++ K            +L +  KD     ++  E+ + GK++ L   E+P+ ++++D EWT E+  +TA+ K++R+ IV  Y ++I+K
Sbjct:   37 EDGVSTVYDLLCTAAKNHGEKQAMGSRKLIKENREEREIIRKVDNEEVVEKKLWTTYELGPYEYISF-NKVYEIALALGSGLVASGITSETTMLFFAATSAKWFTTAQGCSSQAIPIVTAYETLGEDGIYTSLDECKSRAIFTDPNLIPKLLGPLKQSTWVKLIVCSSTPSEDLVELVKSTAPDVE--IITYDNLLSLGKEKPQPPHPPKADDICCYMYTSGSTGKPKGVVLLHRNIIAALGGINRILSQHINVKDYVLAYLPLAHIFEFIFEMCCLYWGGVLGYASPRTLTDASV---VNCKGDLTEFRPTVLIGVPAVYELIKKGILAKVSSMPAHRQKVFSGSLSLKQYLIERNLPGSAALDAIVFNKVKAATGGRLRYCISGGAALAASTQAFLSSCIC-PVLPGYGLTETCAGSFVLSPEQWHLYANTVGFPIPSIEFKLVDIPDLGYYTDSSP-PRGEVWIRGPAVCNGYLNRPEDNKAAF-TEDG--WFKTGDVGEIAKGNTLRLIDRKKNIVKSLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAKPVVIVNPDENGLRTYLTKNSGSSFNGNPNDTLTNLCKDSGVQHLILKELINIGKQQRLASIEIPEGVVLSDFEWTAENNFLTASRKVKRQVIVAHYSDEIQK 683          

BLAST of GK21733 vs. nr
Match: gi|1228370610|ref|XP_021946389.1| (long-chain-fatty-acid--CoA ligase 4-like isoform X2 [Folsomia candida] >gi|1228370612|ref|XP_021946390.1| long-chain-fatty-acid--CoA ligase 4-like isoform X2 [Folsomia candida] >gi|1215272744|gb|OXA59054.1| Long-chain-fatty-acid--CoA ligase 4 [Folsomia candida])

HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 320/703 (45.52%), Postives = 447/703 (63.58%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSR---QPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS-------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK-------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +LL+A+ F       +YD +  PIY +  +PWR  R  K + A+     GG+ F S T  +K++ E+E+QG+DTM++VFNY  ++   + CLG+RE+I+EEDE+Q NGK + KY+LG+Y W+T++ +       S GL+++GLE   K+ I A+TRAEW+I A  CF+++I +VTLY  LG D ++H +NETE   ++ S + M K + +L     VK +I +    P +++      + +      + +  G  S     PP P+D AIIMYTSGSTG PKGV+LS +N+  AM A  + +      D Y+ YLPLAHVLEL+ E+   + GI IGYS+P T+T++SSK+K GCKGD ++LRPT+M SVP++L+RIYK I + +   G   + LF +F  YK+KW  RGF+TPL+N+ +F+K+R  +GGRVR++ SGGAPL+ +TH   R  +CVP+LQGYGLTET ACAT +                     VNWD+G Y + D+PF RGEI IGGD V+LGYYKLPE  EKDF+EEDG RWFR+GDI EVQ DG  KIIDR KDLVKLQ GEY+S GKVES LK C LV+NIC+  D+SK + +A+I+P    LT    K       +L++   D      V  EIQ  G    L + E+P  + +    W P+SGLVTAA K++R+ I   Y++ I +
Sbjct:   10 VLLRAITF-------IYDVLTLPIYTIVQYPWRKVRECKESKAQAISHEGGITFRSTTPISKMHVELEKQGIDTMEKVFNYAVRRNGSKRCLGSRELIDEEDEVQKNGKVFKKYVLGEYLWKTYNEVDRLASGFSKGLRQMGLEPKGKICIFAETRAEWLIAAIGCFKQNITLVTLYATLGEDAVIHGLNETECEFVLTSHDLMPKFRVILENSKSVKHLIYME--DPLRKTDVSKFPENVKIHGFCDVIAAGTKSSIGGSPPSPEDVAIIMYTSGSTGAPKGVLLSQRNVSIAMMAFSDALGPVYPDDIYLGYLPLAHVLELMCESISFLSGIPIGYSTPLTMTDQSSKIKRGCKGDTSVLRPTIMASVPLILDRIYKGIQEKVGESGKIGQALFKFFYDYKLKWYYRGFNTPLVNRLIFKKLRQLIGGRVRILASGGAPLAPDTHEFIRNSLCVPLLQGYGLTETAACATISLGSDMSTGRVGAPLGCCDIRLVNWDEGNYTIKDRPFPRGEILIGGDNVALGYYKLPEATEKDFFEEDGRRWFRSGDIAEVQEDGCFKIIDRKKDLVKLQVGEYVSLGKVESELKTCPLVDNICIYGDASKQFCVALIVPAVKHLTILATKLGKSGDMTLEEMCADAGLANAVLKEIQIHGTNSKLAKFEIPGAVTLCSDTWQPDSGLVTAAFKLKRKNIQDHYQSDINR 703          

BLAST of GK21733 vs. nr
Match: gi|1228370608|ref|XP_021946388.1| (long-chain-fatty-acid--CoA ligase 4-like isoform X1 [Folsomia candida])

HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0
Identity = 320/703 (45.52%), Postives = 447/703 (63.58%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSR---QPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS-------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK-------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +LL+A+ F       +YD +  PIY +  +PWR  R  K + A+     GG+ F S T  +K++ E+E+QG+DTM++VFNY  ++   + CLG+RE+I+EEDE+Q NGK + KY+LG+Y W+T++ +       S GL+++GLE   K+ I A+TRAEW+I A  CF+++I +VTLY  LG D ++H +NETE   ++ S + M K + +L     VK +I +    P +++      + +      + +  G  S     PP P+D AIIMYTSGSTG PKGV+LS +N+  AM A  + +      D Y+ YLPLAHVLEL+ E+   + GI IGYS+P T+T++SSK+K GCKGD ++LRPT+M SVP++L+RIYK I + +   G   + LF +F  YK+KW  RGF+TPL+N+ +F+K+R  +GGRVR++ SGGAPL+ +TH   R  +CVP+LQGYGLTET ACAT +                     VNWD+G Y + D+PF RGEI IGGD V+LGYYKLPE  EKDF+EEDG RWFR+GDI EVQ DG  KIIDR KDLVKLQ GEY+S GKVES LK C LV+NIC+  D+SK + +A+I+P    LT    K       +L++   D      V  EIQ  G    L + E+P  + +    W P+SGLVTAA K++R+ I   Y++ I +
Sbjct:   27 VLLRAITF-------IYDVLTLPIYTIVQYPWRKVRECKESKAQAISHEGGITFRSTTPISKMHVELEKQGIDTMEKVFNYAVRRNGSKRCLGSRELIDEEDEVQKNGKVFKKYVLGEYLWKTYNEVDRLASGFSKGLRQMGLEPKGKICIFAETRAEWLIAAIGCFKQNITLVTLYATLGEDAVIHGLNETECEFVLTSHDLMPKFRVILENSKSVKHLIYME--DPLRKTDVSKFPENVKIHGFCDVIAAGTKSSIGGSPPSPEDVAIIMYTSGSTGAPKGVLLSQRNVSIAMMAFSDALGPVYPDDIYLGYLPLAHVLELMCESISFLSGIPIGYSTPLTMTDQSSKIKRGCKGDTSVLRPTIMASVPLILDRIYKGIQEKVGESGKIGQALFKFFYDYKLKWYYRGFNTPLVNRLIFKKLRQLIGGRVRILASGGAPLAPDTHEFIRNSLCVPLLQGYGLTETAACATISLGSDMSTGRVGAPLGCCDIRLVNWDEGNYTIKDRPFPRGEILIGGDNVALGYYKLPEATEKDFFEEDGRRWFRSGDIAEVQEDGCFKIIDRKKDLVKLQVGEYVSLGKVESELKTCPLVDNICIYGDASKQFCVALIVPAVKHLTILATKLGKSGDMTLEEMCADAGLANAVLKEIQIHGTNSKLAKFEIPGAVTLCSDTWQPDSGLVTAAFKLKRKNIQDHYQSDINR 720          

BLAST of GK21733 vs. nr
Match: gi|1061486181|gb|ODN03288.1| (Long-chain-fatty-acid--CoA ligase 4 [Orchesella cincta])

HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0
Identity = 321/705 (45.53%), Postives = 459/705 (65.11%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSV----GLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILL-NPSSPKKRSRPQLQCQVIPY-DWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIIN-IAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS-------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +L+K +L   +++  ++D++  P +++Y  PW+  R  K   A+     GG+ + S+T  + ++ E+E++ +DTMD+VF Y  K+   + CLG+RE+I+EEDE+QSNGK + KY+LGDY W+T++    EVD +SV    GL+ELGLE   K+A+LA+TRAEW+I A A F++ I +VTLY  LG D +VH +NETEV  ++ S + + K + +L  C  VK++I + +P +    S  +   +++ + D +    ++G  + +P    D AIIMYTSGSTG PKGV+LSH+N+  AM A  + +      D Y+ YLPLAHVLEL+ E  C + GI IGYSS  T+ + SSK+K GCKGDA++LRPT+M SVP+VL+RIYK I + M   G   + +F +F  YKMKW  RG+ TPL N+ +F+ +R  +GG+VRL+ SGGAPL+ + H   RT +C+P+LQGYGLTET ACAT +                     VNWD+G Y V+DKP+ RGEI IGGD V+LGYYK+ E + +DF+EEDG RWFR+GDIGE+Q DGVLKIIDR KDLVKLQ GEY+S GKVE+ LK C +VENIC+  D SK + +A+I+P+   + +   K      S +    DPE  + V  EIQ  G +  L + E+P  + +    W+P+ GLVTAA K++R+ I   Y+  I +
Sbjct:    6 VLIKGIL---WVVTFLWDFLTLPFFYIYQQPWKKTRDFKEPKAQAVSHEGGITYRSITPISAMHIELEQKKIDTMDKVFQYAVKRNGSKRCLGSREVIDEEDEMQSNGKVFKKYVLGDYLWKTYN----EVDRLSVCFSKGLRELGLEPKGKIAVLAETRAEWLISAMAAFRQCITMVTLYATLGEDAIVHGLNETEVDYVLTSHDQLPKFKNVLKHCPKVKTIIYMEDPLNKTDTSGYREDVEILAFCDVIARGSRSGKGTHEPA-ADDIAIIMYTSGSTGAPKGVLLSHRNISIAMMAFSDALGPVYPDDIYLGYLPLAHVLELMCEAICFLSGIPIGYSSALTMIDSSSKIKRGCKGDASVLRPTIMASVPLVLDRIYKGIQEKMNAGGGVAKAVFEFFYQYKMKWYYRGYQTPLTNRLIFKPVRRVVGGKVRLLASGGAPLAPDVHEFVRTSLCLPLLQGYGLTETAACATISLCSDMSTGRVGAPLGCCDIRLVNWDEGNYTVNDKPYPRGEILIGGDNVALGYYKMSESSNQDFFEEDGRRWFRSGDIGEMQKDGVLKIIDRKKDLVKLQFGEYVSLGKVEAELKTCPIVENICIYGDPSKTFCVALIVPNPKYIQTVADKLGRSGLSFEMLCNDPEIEQAVLKEIQSHGAKCRLQKFEIPGAVKLCIEVWSPDMGLVTAAFKLKRKNIQDRYQGDINR 702          

BLAST of GK21733 vs. nr
Match: gi|195430072|ref|XP_002063081.1| (long-chain-fatty-acid--CoA ligase 4 isoform X1 [Drosophila willistoni] >gi|194159166|gb|EDW74067.1| uncharacterized protein Dwil_GK21733, isoform A [Drosophila willistoni])

HSP 1 Score: 615.92 bits (1587), Expect = 0.000e+0
Identity = 302/691 (43.70%), Postives = 442/691 (63.97%), Query Frame = 0

Query:  204 IYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTK-VNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRP-QLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-NNYS------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVR------KSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            I  I  VYD I  P+Y V   PW+ R+  +   A+        + +  TEP + V+ ++ ++ +DT+++VFNY+ K +  + CLGTR+I+ EEDE+Q NG+ + KY +GDYKW+ F+  +    +   GL+ELG +    + I A+TRAEWMI A+ CF++++ +VT+Y  LG+DG+ H I+ETEV+ ++ S + + K + +L  C +V ++I +     K  +   +   +V+P+  ++ + Q       PP   D AIIMYTSGSTGTPKGV+LSHKN ++ MK   ++      D  I +LPLAHV EL+AE+ C+M G+ IGYS+P TL + SSK+K GCKGDA +L+PT M SVP++L+RI K I D +       + LF +   YK+KW  RG+ TPL++K +F+K+   +GGRVR+M+SGGAPLSS+TH   +TC+CV ++QGYGLTETT+ AT  +Y                   VNW++G Y +++KP+ +GE+ IGGD VS GYYKLP +  +DF+EEDG RWF+TGDIGEVQ DGVLKIIDR KDLVKLQ GEY+S GKVES LK C ++ENIC+  D +K + +A+++P++  L           KS +D    P   K +  EI D  ++  L + E+P  I +    W+P+ GLVTAA K++R+ I   Y++ I +
Sbjct:   21 IKAIAFVYDIITLPVYLVLQAPWKRRQDSRRVKAKVIKNDDNELTYRTTEPPRDVHVKMLQENIDTLEKVFNYVAKTHSSKRCLGTRQILSEEDEVQPNGRVFKKYNMGDYKWKNFTEAERMAASFGRGLRELGQKPRENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCISETEVTTVITSHDLLPKFRTLLAQCPNVTTIIYMEDQLHKTETTGFKEGVKVLPFAQVVKTGQDSKFENVPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKGFCDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVSIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSDSAFKKALFKFLYQYKVKWITRGYLTPLIDKLVFKKVAKLMGGRVRIMMSGGAPLSSDTHEQIKTCLCVDLIQGYGLTETTSGATVMDYRDMTYGRTGAPLTVCDIRLVNWEEGNYRITNKPYPQGEVLIGGDCVSQGYYKLPGKTNEDFFEEDGRRWFKTGDIGEVQSDGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQFTVALVVPNQKHLEELAERHGLQNKSFEDLCSSPVMEKAILKEIADHSRKCKLQKFEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINR 711          

BLAST of GK21733 vs. nr
Match: gi|1022759636|gb|KZS05957.1| (Acyl-CoA synthetase long-chain [Daphnia magna])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 306/699 (43.78%), Postives = 456/699 (65.24%), Query Frame = 0

Query:  199 AVLFFIYLINHVYDYICYPIYFVYYHPWRVR----RYKKSTHARREDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILL-NPSSPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT------NNYS-------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            A+L  I  I  +Y ++ +P+YFV   PW++R    R +  +    +D    +++ S+TE + V+    +  +DTM++VF +  +Q+ ++  LGTREI+ EEDE+Q NG+ + K++LGD++WRTF+    E ++   GL+ELGL     + + A+TRAEW+I AN C ++++ +VTLY  LG+D ++H INETEVS ++ S+E + K + +LP   +V  +I++ +P  P   +       V+PY  ++        S   P P D AIIMYTSGSTG+PKGV++SH+N+++AM    N+  F   D Y+AYLPLAHVLEL++E+  M++GI IGYS+P T+T+KSSK+K G +GDA++LRPT++ SVP+VL+RI+KSI + ++      + +F+  M YK +W DRG++TP++N TLF+ IR  LGGR+R +LSGGAPL+ ETH   +T +C PVLQGYGLTET A  T      N+               V+W++G Y + D P  RGEIHIGG  V++GYYK PE+ +++FYEE+G  WFRTGDIGE   +G L+IIDR KDLVKLQ GEY+S GKVE+ LK   LVENIC+ AD +K + +A+++P+ D L S   K      ++++    PE  K+V  E+Q    +  L + E+PQ + +    WTP+SGLVTAA K++R+ +   Y   I++
Sbjct:   10 AILLLIRCIISIYTFVTWPVYFVLQKPWKIRANRSRLRAVSLTHGDDF---ILYRSVTEISDVHARFLQDKIDTMEKVFRFAVRQHGQKCALGTREILAEEDEMQPNGRMFKKFLLGDFQWRTFTQTDSEAESFGRGLRELGLRSRENVVVFAETRAEWLISANGCMKQNMPIVTLYATLGDDAIIHGINETEVSCVITSQELLPKFKNILPCTPNVTLLIVMEDPLKPLDLNGYGDTVAVLPYQEVIRLGDLSSKSGCLPKPDDTAIIMYTSGSTGSPKGVIMSHQNVVNAMLGYTNVTTFYENDVYMAYLPLAHVLELISESIWMLFGIPIGYSTPLTMTDKSSKIKRGSQGDASVLRPTIIASVPLVLDRIHKSIQEKIEGGPAITKAVFNLAMEYKNEWTDRGYETPIINTTLFKPIRALLGGRLRFVLSGGAPLAPETHKSLKTTLCCPVLQGYGLTETCAATTVMDPDDNSLGMTGGPLTCCDVKLVSWEEGNYRIKDWPNPRGEIHIGGTNVAVGYYKQPEKTKEEFYEENGRHWFRTGDIGEFTQEGFLQIIDRKKDLVKLQYGEYVSLGKVETQLKINPLVENICIYADPTKLFPVALLVPNFDHLKSLAEKNGIELNTIEELCSHPEMAKLVLGELQKHAVKCRLQKFEIPQALTLIPDIWTPDSGLVTAAFKLKRKTVQDRYEASIDR 705          

BLAST of GK21733 vs. nr
Match: gi|1286077212|ref|XP_023030526.1| (long-chain-fatty-acid--CoA ligase 4 isoform X2 [Drosophila willistoni] >gi|946581066|gb|KRF97893.1| uncharacterized protein Dwil_GK21733, isoform B [Drosophila willistoni])

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 302/691 (43.70%), Postives = 442/691 (63.97%), Query Frame = 0

Query:  204 IYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHARREDIPGGVIFHSLTEPTK-VNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRP-QLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT-NNYS------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVR------KSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            I  I  VYD I  P+Y V   PW+ R+  +   A+        + +  TEP + V+ ++ ++ +DT+++VFNY+ K +  + CLGTR+I+ EEDE+Q NG+ + KY +GDYKW+ F+  +    +   GL+ELG +    + I A+TRAEWMI A+ CF++++ +VT+Y  LG+DG+ H I+ETEV+ ++ S + + K + +L  C +V ++I +     K  +   +   +V+P+  ++ + Q       PP   D AIIMYTSGSTGTPKGV+LSHKN ++ MK   ++      D  I +LPLAHV EL+AE+ C+M G+ IGYS+P TL + SSK+K GCKGDA +L+PT M SVP++L+RI K I D +       + LF +   YK+KW  RG+ TPL++K +F+K+   +GGRVR+M+SGGAPLSS+TH   +TC+CV ++QGYGLTETT+ AT  +Y                   VNW++G Y +++KP+ +GE+ IGGD VS GYYKLP +  +DF+EEDG RWF+TGDIGEVQ DGVLKIIDR KDLVKLQ GEY+S GKVES LK C ++ENIC+  D +K + +A+++P++  L           KS +D    P   K +  EI D  ++  L + E+P  I +    W+P+ GLVTAA K++R+ I   Y++ I +
Sbjct:   13 IKAIAFVYDIITLPVYLVLQAPWKRRQDSRRVKAKVIKNDDNELTYRTTEPPRDVHVKMLQENIDTLEKVFNYVAKTHSSKRCLGTRQILSEEDEVQPNGRVFKKYNMGDYKWKNFTEAERMAASFGRGLRELGQKPRENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCISETEVTTVITSHDLLPKFRTLLAQCPNVTTIIYMEDQLHKTETTGFKEGVKVLPFAQVVKTGQDSKFENVPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKGFCDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVSIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSDSAFKKALFKFLYQYKVKWITRGYLTPLIDKLVFKKVAKLMGGRVRIMMSGGAPLSSDTHEQIKTCLCVDLIQGYGLTETTSGATVMDYRDMTYGRTGAPLTVCDIRLVNWEEGNYRITNKPYPQGEVLIGGDCVSQGYYKLPGKTNEDFFEEDGRRWFKTGDIGEVQSDGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQFTVALVVPNQKHLEELAERHGLQNKSFEDLCSSPVMEKAILKEIADHSRKCKLQKFEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINR 703          

BLAST of GK21733 vs. nr
Match: gi|482683418|ref|XP_001361520.3| (uncharacterized protein Dpse_GA21288, isoform B [Drosophila pseudoobscura pseudoobscura] >gi|1036995579|ref|XP_017149179.1| PREDICTED: long-chain-fatty-acid--CoA ligase 4 isoform X3 [Drosophila miranda] >gi|478540125|gb|EAL26098.3| uncharacterized protein Dpse_GA21288, isoform B [Drosophila pseudoobscura pseudoobscura])

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 297/699 (42.49%), Postives = 449/699 (64.23%), Query Frame = 0

Query:  197 LKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHAR--REDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPSSPKKRSRP-QLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACATNNYS-------------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVR------KSLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            +++ +  I  I  VYD I  P+Y V   PW+ R+  +   A+  ++D    + + +   P  V+ ++ ++ +DT+++VFNY+ K +  + CLGTR+I+ EEDE+Q NG+ + KY +GDYKWRTF+  + +      GL+ELG +    + I A+TRAEWM+ A+ CF++++ +VT+Y  LG+DG+ H I+ETEV+ ++ S + + K + +L  C +V ++I +     K  +   +   +V+P++ ++   Q       PP   D AIIMYTSGSTGTPKGV+LSHKN ++ MK  +++      D  I +LPLAHV EL+AE+ C+M G+ IGYS+P TL + SSK++ GCKGDA++L+PT M SVP++L+RI K I D +       + LF +   YK+KW  RG+ TPL++K +F+K+   +GG+VR+++SGGAPLS++TH   +TC+CV ++QGYGLTETT+ AT  +                    VNW++G Y V++KP+ +GE+ IGGD VS GYYKLP +  +DF+EEDG RWF+TGDIGEVQ DGVLKIIDR KDLVKLQ GEY+S GKVES LK C +VENIC+  D +K + +A+++P++  L           K+ ++    P   K +  EI D  ++  L + E+P  I +    W+P+ GLVTAA K++R+ I   Y++ I +
Sbjct:   14 VQSAIGAIKAIAFVYDIITLPVYLVLQKPWQRRQDSRRVKAKIIKKD-ESELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTHSNKRCLGTRQILSEEDEVQPNGRVFKKYNMGDYKWRTFTEAERQAANFGRGLRELGQKPRENIVIFAETRAEWMVAAHGCFKQAMPIVTVYATLGDDGVAHCISETEVTTVITSHDLLPKFKTLLAQCPNVDTIIYMEDQLHKTETTGFKEGVKVLPFNQVVKIGQDSKFESVPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKGFVDMVPIYPDDVLIGFLPLAHVFELVAESLCLMTGVSIGYSTPLTLIDTSSKIRRGCKGDASVLKPTCMTSVPLILDRISKGINDKVNSGSAFRKALFKFLYQYKVKWIQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCVDLIQGYGLTETTSGATVMHERDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGDCVSQGYYKLPSKTNEDFFEEDGKRWFKTGDIGEVQTDGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIVENICVYGDPTKQFTVALVVPNQKHLEDLAERHGLQNKTYEELCSSPVMEKAILKEIADHSRKCKLQKFEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINR 711          

BLAST of GK21733 vs. nr
Match: gi|58384648|ref|XP_313383.2| (AGAP003623-PA [Anopheles gambiae str. PEST] >gi|55241100|gb|EAA08767.2| AGAP003623-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0
Identity = 301/701 (42.94%), Postives = 442/701 (63.05%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHAR--REDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPS-SPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT------NNYS-------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            + +++ +  I +I  VYD I  PIY     PW+ R+  +   A+  ++D    + + S+  P +++ ++ +  +DT++++FN++ K +  + C+GTR+I+ EEDE+Q NG+ + K+ +GDY WR F   ++       GL+ELG E    + I A+TRAEWMI A+ CF++++ VVT+Y  LG+DG+ H INETEV+ ++ S E + K + +L    +VK +I +     P   +  +   ++IP+  ++    T      PP  +D AIIMYTSGSTGTPKGV+LSH N +  MK   +I +    D  I +LPLAHV ELLAE+ C++ G+ IGYS+P TL + SSKV  GCKGDA++LRPT M SVP++L+RI K I D +  +    +  F +  +YK KW  RG+ TPL++K LF+KI   LGGR+R +LSGGAPL+ +TH   + C+CV V+QGYGLTETTA A         YS             +NW++G Y V++KP+ +GEI +GG  VS GYYKLP + ++DF+EEDG RWFRTGD+GE+  DG LKIIDR KDLVKLQ GEY+S GKVES LK C +VENIC+  DS+K Y +A+++P+   L     +        +D   + +  K V  E+ D G++  L+R+E+P  + +    WTP+ GLVTAA K++R+ I   Y+ +I K
Sbjct:    4 VWVQSAIGAIKIITMVYDIITMPIYLAIQRPWKRRQLARRVKAKIIQQD-SSSITYRSVDSPGEMHVKMMQNNIDTLERMFNFVTKVHTTKRCIGTRQILGEEDEMQPNGRMFKKFRMGDYVWRNFIETEHAAACFGRGLRELGQEPKQNIVIFAETRAEWMIAAHGCFKQNMPVVTIYATLGDDGVAHGINETEVTTVITSHELLPKFKSVLNVTPNVKKIIFMEDQLHPTDTTGFKEGVEIIPFSKVIEIGNTSTIPGSPPAAEDTAIIMYTSGSTGTPKGVLLSHANCIGTMKNFCDIFKIYPDDVLIGFLPLAHVFELLAESVCLLTGVPIGYSTPLTLIDSSSKVMKGCKGDASVLRPTCMTSVPLILDRISKGINDKVNAEAPMKKAFFKFAYNYKSKWTARGYQTPLMDKILFKKIAKLLGGRIRSVLSGGAPLAPDTHEQIKLCLCVDVIQGYGLTETTAGAAVMDKWDMEYSRVGAPSSSNDIRLINWEEGNYRVTNKPYPQGEIVVGGTTVSKGYYKLPGKTQEDFFEEDGQRWFRTGDVGEIHPDGALKIIDRKKDLVKLQAGEYVSLGKVESELKTCPVVENICVYGDSTKQYTVALVVPNPKHLEEIAERLDIRGVEFEDLCDNKKLEKAVLQELADHGRKCKLHRSEIPAAVHLCKDIWTPDMGLVTAAFKLKRKDIQERYQAEISK 703          

BLAST of GK21733 vs. nr
Match: gi|347970251|ref|XP_003436541.1| (AGAP003623-PB [Anopheles gambiae str. PEST] >gi|333468844|gb|EGK97082.1| AGAP003623-PB [Anopheles gambiae str. PEST])

HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0
Identity = 302/708 (42.66%), Postives = 444/708 (62.71%), Query Frame = 0

Query:  188 KSASAKYILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHAR--REDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPS-SPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT------NNYS-------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            K    + + +++ +  I +I  VYD I  PIY     PW+ R+  +   A+  ++D    + + S+  P +++ ++ +  +DT++++FN++ K +  + C+GTR+I+ EEDE+Q NG+ + K+ +GDY WR F   ++       GL+ELG E    + I A+TRAEWMI A+ CF++++ VVT+Y  LG+DG+ H INETEV+ ++ S E + K + +L    +VK +I +     P   +  +   ++IP+  ++    T      PP  +D AIIMYTSGSTGTPKGV+LSH N +  MK   +I +    D  I +LPLAHV ELLAE+ C++ G+ IGYS+P TL + SSKV  GCKGDA++LRPT M SVP++L+RI K I D +  +    +  F +  +YK KW  RG+ TPL++K LF+KI   LGGR+R +LSGGAPL+ +TH   + C+CV V+QGYGLTETTA A         YS             +NW++G Y V++KP+ +GEI +GG  VS GYYKLP + ++DF+EEDG RWFRTGD+GE+  DG LKIIDR KDLVKLQ GEY+S GKVES LK C +VENIC+  DS+K Y +A+++P+   L     +        +D   + +  K V  E+ D G++  L+R+E+P  + +    WTP+ GLVTAA K++R+ I   Y+ +I K
Sbjct:    4 KQKDMESVWVQSAIGAIKIITMVYDIITMPIYLAIQRPWKRRQLARRVKAKIIQQD-SSSITYRSVDSPGEMHVKMMQNNIDTLERMFNFVTKVHTTKRCIGTRQILGEEDEMQPNGRMFKKFRMGDYVWRNFIETEHAAACFGRGLRELGQEPKQNIVIFAETRAEWMIAAHGCFKQNMPVVTIYATLGDDGVAHGINETEVTTVITSHELLPKFKSVLNVTPNVKKIIFMEDQLHPTDTTGFKEGVEIIPFSKVIEIGNTSTIPGSPPAAEDTAIIMYTSGSTGTPKGVLLSHANCIGTMKNFCDIFKIYPDDVLIGFLPLAHVFELLAESVCLLTGVPIGYSTPLTLIDSSSKVMKGCKGDASVLRPTCMTSVPLILDRISKGINDKVNAEAPMKKAFFKFAYNYKSKWTARGYQTPLMDKILFKKIAKLLGGRIRSVLSGGAPLAPDTHEQIKLCLCVDVIQGYGLTETTAGAAVMDKWDMEYSRVGAPSSSNDIRLINWEEGNYRVTNKPYPQGEIVVGGTTVSKGYYKLPGKTQEDFFEEDGQRWFRTGDVGEIHPDGALKIIDRKKDLVKLQAGEYVSLGKVESELKTCPVVENICVYGDSTKQYTVALVVPNPKHLEEIAERLDIRGVEFEDLCDNKKLEKAVLQELADHGRKCKLHRSEIPAAVHLCKDIWTPDMGLVTAAFKLKRKDIQERYQAEISK 710          

BLAST of GK21733 vs. nr
Match: gi|347970249|ref|XP_003436540.1| (AGAP003623-PC [Anopheles gambiae str. PEST] >gi|333468845|gb|EGK97083.1| AGAP003623-PC [Anopheles gambiae str. PEST])

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 301/701 (42.94%), Postives = 442/701 (63.05%), Query Frame = 0

Query:  195 ILLKAVLFFIYLINHVYDYICYPIYFVYYHPWRVRRYKKSTHAR--REDIPGGVIFHSLTEPTKVNREIEEQGLDTMDQVFNYICKQYLEQDCLGTREIIEEEDEIQSNGKTYVKYILGDYKWRTFSNMKYEVDAVSVGLKELGLERGSKLAILADTRAEWMILANACFQRSIVVVTLYTNLGNDGLVHAINETEVSVLVCSEESMAKVQEMLPACGHVKSVILLNPS-SPKKRSRPQLQCQVIPYDWLLNSMQTGDPSRQPPLPQDCAIIMYTSGSTGTPKGVMLSHKNLLSAMKAIINIAEFRAGDRYIAYLPLAHVLELLAETSCMMYGIKIGYSSPATLTNKSSKVKAGCKGDANILRPTLMCSVPMVLERIYKSIVDTMKRQGWAVEELFHYFMSYKMKWEDRGFDTPLLNKTLFRKIRYFLGGRVRLMLSGGAPLSSETHSLTRTCICVPVLQGYGLTETTACAT------NNYS-------------VNWDDGGYFVSDKPFSRGEIHIGGDIVSLGYYKLPERNEKDFYEEDGVRWFRTGDIGEVQGDGVLKIIDRMKDLVKLQQGEYISCGKVESILKNCFLVENICLVADSSKDYLIAIILPDKDQLTSFVRK------SLQDCAKDPECLKMVALEIQDFGKRKGLNRTELPQKIIIADGEWTPESGLVTAALKIRRRQIVMEYRNQIEK 867
            + +++ +  I +I  VYD I  PIY     PW+ R+  +   A+  ++D    + + S+  P +++ ++ +  +DT++++FN++ K +  + C+GTR+I+ EEDE+Q NG+ + K+ +GDY WR F   ++       GL+ELG E    + I A+TRAEWMI A+ CF++++ VVT+Y  LG+DG+ H INETEV+ ++ S E + K + +L    +VK +I +     P   +  +   ++IP+  ++    T      PP  +D AIIMYTSGSTGTPKGV+LSH N +  MK   +I +    D  I +LPLAHV ELLAE+ C++ G+ IGYS+P TL + SSKV  GCKGDA++LRPT M SVP++L+RI K I D +  +    +  F +  +YK KW  RG+ TPL++K LF+KI   LGGR+R +LSGGAPL+ +TH   + C+CV V+QGYGLTETTA A         YS             +NW++G Y V++KP+ +GEI +GG  VS GYYKLP + ++DF+EEDG RWFRTGD+GE+  DG LKIIDR KDLVKLQ GEY+S GKVES LK C +VENIC+  DS+K Y +A+++P+   L     +        +D   + +  K V  E+ D G++  L+R+E+P  + +    WTP+ GLVTAA K++R+ I   Y+ +I K
Sbjct:   12 VWVQSAIGAIKIITMVYDIITMPIYLAIQRPWKRRQLARRVKAKIIQQD-SSSITYRSVDSPGEMHVKMMQNNIDTLERMFNFVTKVHTTKRCIGTRQILGEEDEMQPNGRMFKKFRMGDYVWRNFIETEHAAACFGRGLRELGQEPKQNIVIFAETRAEWMIAAHGCFKQNMPVVTIYATLGDDGVAHGINETEVTTVITSHELLPKFKSVLNVTPNVKKIIFMEDQLHPTDTTGFKEGVEIIPFSKVIEIGNTSTIPGSPPAAEDTAIIMYTSGSTGTPKGVLLSHANCIGTMKNFCDIFKIYPDDVLIGFLPLAHVFELLAESVCLLTGVPIGYSTPLTLIDSSSKVMKGCKGDASVLRPTCMTSVPLILDRISKGINDKVNAEAPMKKAFFKFAYNYKSKWTARGYQTPLMDKILFKKIAKLLGGRIRSVLSGGAPLAPDTHEQIKLCLCVDVIQGYGLTETTAGAAVMDKWDMEYSRVGAPSSSNDIRLINWEEGNYRVTNKPYPQGEIVVGGTTVSKGYYKLPGKTQEDFFEEDGQRWFRTGDVGEIHPDGALKIIDRKKDLVKLQAGEYVSLGKVESELKTCPVVENICVYGDSTKQYTVALVVPNPKHLEEIAERLDIRGVEFEDLCDNKKLEKAVLQELADHGRKCKLHRSEIPAAVHLCKDIWTPDMGLVTAAFKLKRKDIQERYQAEISK 711