GF14256, maker-scaffold722_size106786-snap-gene-0.21 (gene) Tigriopus kingsejongensis

Overview
NameGF14256
Unique Namemaker-scaffold722_size106786-snap-gene-0.21
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000011568 (supercontig:LSalAtl2s:LSalAtl2s800:154479:160331:1 gene:EMLSAG00000011568 transcript:EMLSAT00000011568 description:"maker-LSalAtl2s800-augustus-gene-1.53")

HSP 1 Score: 1441.79 bits (3731), Expect = 0.000e+0
Identity = 798/1541 (51.78%), Postives = 1024/1541 (66.45%), Query Frame = 0
Query:    2 FLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHP-SLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASA--VSLPSHEPETASEAVHVVRPILLDLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLP-EGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQA--------EKDAVPETPEATDED-PAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMK-GTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSG---------------------------HGP----DGLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISD-NKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSF---------EESWWRDLYQVLFR-------------------IFEVGK---------------------FD---MNDLSLRDHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDTSVIVYKEEDIVFGRVHVHLDVLEVSMNVLTSTAYGQTIEGPPWFEVLMPDHFQVLKKLLRSLEQSFNESKSVLEDTPNSSRKRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDIPR 1437
            F+ K+IESLL++K+++R    ELR ACE AL     L  +    G+  VLP       I +   L P ELAC+ SK+  +V  SLDS+QKLVAYG  RF+          G P +  D+L  SVAHAFSGPQTD +VQLQV+KALLTI+TSAN+ VH+NSLLL+V+TC+NI+LA RN+INQT AKATL+Q+LN +FS ME+AS    ++P       +++   V P++ +L D ++        +++ ++Q D +LV RSLCKLS+ P P +   D KSH LRSKILSL LLLSILQN G+ F  + IF+S +K YLCVALS NGVS +SEVFELS+A+FIS+LT+YR +LK QIEIFFKEICLNILEA+SSSFEQKW V+QG   IC D QIV+DIFVNYDCDL AANIFERLVDDL+K+AQG+QA+++GATP Q+ ++RI+ LECL  ILKC+VEWSKELYVNPH             +  E P   + D P+  E+VKQ K VLE GI+LF  KP KGI+YL    I       +A F H E NRL+KT +GEFLG+LENKE MY YVD ++F  ++FV ALR FL  FRLPGEAQKIDRLMEKFASRY +CNPTQ LF SAD AYVLAYS+IMLTTDLHS+ VKKKMTKEEF+RNN+GINDSED+P +YLS IY+ IAS+EIKM+  TS       + D K+R  +W+ ES +ISQTAGALMESA +R+D FTSA+ LDHVKPMFKL+WSP+LA FSVGLQ  DD  + QLCL GI+C IR++ IF FSLER+AFIQALARFTLLT++SNV E+++KNI+ IKTLIS+AH+DGNYL  SWL+IMKCISQLE+AQ++G++G                           +GP    DG   +AR S       +EANSQ +IVAVDRIFTGSKNL+G+AIV F  ALC VS++EI S+   RM+SL+KLVEISYYNMERIRL+WSRIW V+GNHFN+VGC + QN+SFFAVDSLKQLSLKFLEKGEL NFHFQKDFLRPFE+I+KNNKS QIRDMVVRC++QM+QSQ++NIKSGWKNI +TF LAAGD+  ++V LS Q+   IV  ++ D N+  + + FQDC+ C+     N    +I   +L  +    + V +    F         +  W R  + +LF                    +FE+ K                     FD   ++D       EWIDTTCNHALYA TDVF+EF+ VL  ++L DLLS YQWCI Q N+QLSKSAISC + LV TN+  ++ ++  LI+ FL D++  T     +       R+ +HLDV+    + + S  + +         +   ++ +++  L+ S  +S  E  S +ED      K  L +L K ET +  C L IL E       +     L    +  L     Y+LSMT+K Q++ W+ +L      +    +  FI  M  LYN  CDILS  +S E + ++C+++KR+G  +DI +
Sbjct:    4 FMRKSIESLLKDKEIRRSHNAELRRACEEAL---EILKDNQVEVGESGVLPTPGAKSPIIDSVFL-PLELACK-SKSAGIVTCSLDSIQKLVAYG--RFN----------GAPRAFYDKLTESVAHAFSGPQTDAEVQLQVIKALLTIVTSANISVHENSLLLAVKTCFNIFLAGRNMINQTVAKATLNQMLNNIFSKMEAASRDFKNIPPLPLLKDTDSHRAVTPLISELIDKVIDPTEEHTHIHK-VLQNDCFLVLRSLCKLSIAPXPSDETSDNKSHGLRSKILSLHLLLSILQNGGETFTSDHIFISVLKQYLCVALSVNGVSIISEVFELSIAIFISILTQYRKYLKSQIEIFFKEICLNILEAASSSFEQKWKVIQGXSKICEDPQIVVDIFVNYDCDLNAANIFERLVDDLSKIAQGRQAFDVGATPLQVKKIRIKGLECLVSILKCLVEWSKELYVNPHYKPMVTENGHHAPSKEEEPNLENSDHPSQYEKVKQHKDVLEQGIRLFNQKPSKGIRYLFNRQICQDNSNSIASFFHSENNRLNKTTLGEFLGDLENKEIMYSYVDLMDFGSINFVKALRHFLEGFRLPGEAQKIDRLMEKFASRYFQCNPTQDLFASADTAYVLAYSIIMLTTDLHSSQVKKKMTKEEFIRNNRGINDSEDLPEDYLSNIYEEIASSEIKMRSNTSEVGKTGFVADQKKRNQIWSMESVNISQTAGALMESACTRSDVFTSARHLDHVKPMFKLLWSPVLAAFSVGLQDCDDAEISQLCLGGIQCAIRISCIFGFSLERNAFIQALARFTLLTDSSNVTEIKAKNIDTIKTLISIAHSDGNYLSSSWLZIMKCISQLEFAQMVGTNGSMNGSNYSISAGSLISAASSKALSLAENGPKLSSDGDYYNARESL------NEANSQSVIVAVDRIFTGSKNLNGDAIVDFVKALCQVSRDEISSEPKPRMFSLIKLVEISYYNMERIRLQWSRIWAVLGNHFNVVGCNESQNISFFAVDSLKQLSLKFLEKGELDNFHFQKDFLRPFEFIVKNNKSTQIRDMVVRCIAQMVQSQARNIKSGWKNILSTFTLAAGDEDGAVVTLSFQSILYIVNSMLDDTNEITVVDFFQDCIKCLSEYACNVLFPEIAMEALQHVRKCANFVAERKSKFLNNDETDDQDRVWIRGWFPILFELSCVMNRSKLDIRTRSLTILFEIVKNYGGLFEKHWWRDLFQVIFRIFDVGKIDDERSSHQNEWIDTTCNHALYATTDVFNEFFRVLSSILLLDLLSLYQWCISQENDQLSKSAISCLKNLVVTNEKLIDQESESLILKFLGDVVSATFANGSRPS---LNRIVIHLDVI----STVKSVVFNRLENNSSVISLRDENYIKIIDNLVMS-HRSAKELMSRMED------KTMLPVLTKYETSSLTCCLEILFEFYVDESSD-----LNSKLLDILRESFSYYLSMTSKLQKDEWNSLLLVTFNHLYTVNDEKFIQLMPELYNHTCDILSSHISNELKVIICKVMKRVGLCFDIVK 1501          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000011656 (supercontig:LSalAtl2s:LSalAtl2s80:773194:804518:1 gene:EMLSAG00000011656 transcript:EMLSAT00000011656 description:"maker-LSalAtl2s80-augustus-gene-8.32")

HSP 1 Score: 159.073 bits (401), Expect = 1.075e-41
Identity = 84/184 (45.65%), Postives = 122/184 (66.30%), Query Frame = 0
Query:  495 LEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKM 676
            L+ G K F   P KGI++L+   ++ +  ++VAQFL+K    L+KT IG+FLGE    N+  +  +V+  +F +L  V ALR FL +FRLPGEAQKIDR+ME FA RY + NP   +F ++D  YVL++++IML T LH+ SVK K + E+F+  N+GIND  D+P E L  +YD+I +   K+
Sbjct:   87 LQMGKKKFNMDPKKGIEFLITQGLIKETPEDVAQFLYK-GEGLNKTMIGDFLGEKNDFNEAVLKAFVELHDFTDLILVQALRQFLWSFRLPGEAQKIDRMMESFAQRYCQLNPD--IFTNSDTCYVLSFAIIMLNTSLHNPSVKDKPSVEQFISMNRGINDGGDLPRELLVSLYDSIKTEPFKI 267          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000006288 (supercontig:LSalAtl2s:LSalAtl2s342:169461:179750:-1 gene:EMLSAG00000006288 transcript:EMLSAT00000006288 description:"maker-LSalAtl2s342-snap-gene-1.11")

HSP 1 Score: 158.688 bits (400), Expect = 4.393e-39
Identity = 153/595 (25.71%), Postives = 267/595 (44.87%), Query Frame = 0
Query:  284 LSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSS--FEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMR-----IRCLECLCFILKCMVEWSKELYVNPHQAEK------DAVPETPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSK--GDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKK---MTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLW----NRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNE---------MRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLL 847
            ++L LL + L+      +  +  ++ +K+ L   +++   S    +F  +L +   + + +R HLK Q+E+FF+++ ++++ + S    +E K   +  +  +      +  +++NYDCD+  +NIFE    DL K+        +G      + +      +  +E  C   K M++  K      H  +K      D    TP+ T      L  +K +K ++    + F  KP KGI ++ E  +        EVA FL +E   LDK  +G+++   +N   +  +V   +F  +    ALR+FL  FRLPGEA  I  +ME FA  + + N       S+ +A+ LAY+VIML  D H+ +  KK   MT E+F RN +G N   D   + L+++Y  I +NEI M            + + +   LW     R  ES        +ES +            DH   +F +IW P +A  S           ++  L G + C  +A  ++     D  I +L +FT L    N+N+         +  K +   + ++S+    G+ L+  W +IM+C+ QL  + LL
Sbjct:  395 VALSLLATALEIGAKDIEKFESLLNLVKNDLSKYVTRLISSESRGIFSSNLRIAFLIFSFFRRHLKFQLEVFFQKL-MDVITSESIKVVYEHKESALDLVVRLYRLPGFITQVYLNYDCDIYTSNIFE----DLTKMLSKNTFPLMGLHSTHFLSLDALLAVVDSIEGHCH--KLMLDVKKGHL---HSQDKGSLQGNDNSSITPKTTLTH-EQLMALKHKKKLIGTATEQFNTKPSKGISFMQEAGLFQNPLDPVEVAHFL-RENPHLDKKQLGDYISNRKNLNILGAFVKSFDFEHVRIDEALRLFLETFRLPGEAPLITLIMEHFADHWQKSNGDP--LSSSXSAFXLAYAVIMLNVDQHNVNHTKKNDPMTMEQFCRNLRGTNGGADHKEDMLAEVYHNIRNNEIVMPAEQ--------SGVVKENYLWKCVLKRGMES------DFLESPNG---------TFDH--ELFGIIWGPTIAALSYVFDKASSEHPLEKSLGGFQKCATIAAHYSMIDVFDNLIISLCKFTTLLSVENLNKNSPFLVSYGLNEKALSVTRLVVSLVIKHGDILREGWKNIMECLLQLFRSGLL 950          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000001923 (supercontig:LSalAtl2s:LSalAtl2s1330:63988:71857:1 gene:EMLSAG00000001923 transcript:EMLSAT00000001923 description:"augustus_masked-LSalAtl2s1330-processed-gene-0.1")

HSP 1 Score: 140.198 bits (352), Expect = 3.386e-34
Identity = 76/191 (39.79%), Postives = 111/191 (58.12%), Query Frame = 0
Query:  491 RKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE---NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNP-TQKLFKSADAAYVLAYSVIMLTTDLHSTSVK--KKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIK 675
            RK     G+ +F  KP KGI YL+    +    + VA+FL      L K  IGE+LG L    N   +  +  E++F  L    ALR F   FR+PGEAQKI+RLME F+ RY++CNP      +S D  ++LA+++I+L TDLH+ ++K  K+M  ++F +N + I+D  D+ PE+L  IY+ I   E K
Sbjct:  288 RKRHYRTGLNIFNKKPEKGISYLIRRGFLENSPQAVARFLITRKG-LSKQMIGEYLGNLTYSFNMAVLACFAGELDFTNLQIDVALRKFQTYFRMPGEAQKIERLMEIFSGRYLQCNPDIGSKLRSTDTIFILAFAIILLNTDLHTPNIKPEKRMKLDDFTKNLRNIDDGRDLDPEWLXGIYERIQGQEFK 477          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000003216 (supercontig:LSalAtl2s:LSalAtl2s177:439240:441692:1 gene:EMLSAG00000003216 transcript:EMLSAT00000003216 description:"maker-LSalAtl2s177-augustus-gene-4.28")

HSP 1 Score: 130.568 bits (327), Expect = 4.440e-31
Identity = 78/230 (33.91%), Postives = 128/230 (55.65%), Query Frame = 0
Query:  458 ELYVNPHQAEKDAVPETPEATDEDPAHLERVKQ------RKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE---NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPT-QKLFKSADAAYVLAYSVIMLTTDLHSTSVK--KKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIK 675
            ++YV P Q +   + +T         H+  ++       RK     G+ LF   P +G++YL+  + V      VA+FL      + K  IGE+L  L+   N   ++ +V EI+F+ L    ALR+      LPGE+QKI++++E F+ RY++CN      FKS D  +VL+Y++I+L TDLHS +V+  ++M KE+F+RN +GI+   D+  + L  I+D I +NE +
Sbjct:  294 DIYVPPAQKKSVRIQDTHNYLPPPKQHINTLRPHVDDTIRKRQYRVGLNLFNQNPERGMEYLIRKNYVEYSPAAVAKFLIGRKG-ISKKMIGEYLTSLQRPFNMAVLHCFVHEIDFSGLHIDIALRILQQEVMLPGESQKIEKMVEVFSKRYIQCNQMFVASFKSTDTIFVLSYAMILLNTDLHSRAVRSSRRMKKEDFVRNLRGIDGGNDVDEDMLRGIFDRIRTNEFR 522          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000005438 (supercontig:LSalAtl2s:LSalAtl2s292:598840:621289:-1 gene:EMLSAG00000005438 transcript:EMLSAT00000005438 description:"maker-LSalAtl2s292-augustus-gene-6.47")

HSP 1 Score: 91.2781 bits (225), Expect = 1.739e-18
Identity = 48/118 (40.68%), Postives = 72/118 (61.02%), Query Frame = 0
Query:  558 YVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQK-LFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEI 674
            Y+   +F+ L    ALR+FL  F L GE Q+ +R++  F+ RY+ECNP  K  F S+DA + L  ++++L TDLH  S ++KMT  EF++N   +N+ +D P E L  +Y AI    I
Sbjct: 1227 YLKFFDFSNLTLDGALRLFLSQFCLTGETQERERVLFYFSKRYMECNPECKSKFLSSDAVHTLTCAIMLLNTDLHGDSGQRKMTSGEFIKNLSELNEGQDFPRELLKSLYYAIKHEPI 1344          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000008391 (supercontig:LSalAtl2s:LSalAtl2s512:629114:630490:1 gene:EMLSAG00000008391 transcript:EMLSAT00000008391 description:"maker-LSalAtl2s512-augustus-gene-5.15")

HSP 1 Score: 78.1814 bits (191), Expect = 1.262e-15
Identity = 53/186 (28.49%), Postives = 92/186 (49.46%), Query Frame = 0
Query:  488 VKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGE-AQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNK---GINDSEDIPPEYLSKIYDAI 669
             K+   +L+ G   F     KG+ Y   + ++    +++AQF +   + L K+ + ++L +  +      Y+   NF+     +ALR F      P +  + + +L+  F+ R+ +CNP  KL  S D  YV  YS+I+L+ DL S  VK KM+K EF+RN +   G +       E   ++YD +
Sbjct:   89 AKKLYLLLDEGTLTFNSDFQKGMDYFFAHGLLKDNTEDIAQFFNGS-HLLSKSEMKKYLQKCTDVTDRLXYLQ--NFSSQSLPNALRKFFSKLEAPDDRGRYLQQLLNTFSRRFCQCNP--KLGYSVDFVYVSCYSLILLSVDLSSPHVKNKMSKREFIRNTRNAVGNDRPLSTGDEIFGEMYDNV 269          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000006779 (supercontig:LSalAtl2s:LSalAtl2s37:479446:498428:1 gene:EMLSAG00000006779 transcript:EMLSAT00000006779 description:"maker-LSalAtl2s37-augustus-gene-5.5")

HSP 1 Score: 61.2326 bits (147), Expect = 2.188e-9
Identity = 41/151 (27.15%), Postives = 71/151 (47.02%), Query Frame = 0
Query:  944 RIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEK-GELQNFHFQKDFLRPFEYIMKNNKSP-QIRDMVVRCLSQMIQSQSQNIKSGWKNIFAT-----FGLAAGDDVESIVALSHQTTQRIVTKLIS-DNKFALKESFQDCLSCVI 1086
            R W +VG HF    C +D  +   AV  +  ++  FL+   EL  FHF +   +PFE I+        I+D VV  + + ++S + +I+SGW+ +F T     F +    D+  I     ++   +    +S D+      +  DC+ C+I
Sbjct: 1085 RAWAIVGPHFMEAACHNDNLIXKKAVQHIHDITTAFLQNISELPFFHFNEAVFKPFENIVCLELCDLDIQDQVVSSICEFVESYTSDIRSGWRPLFGTLKAVQFSIVMESDISHI-----RSVLDVFNAFLSTDSPEIFAYAALDCVGCLI 1230          
BLAST of GF14256 vs. L. salmonis genes
Match: EMLSAG00000006002 (supercontig:LSalAtl2s:LSalAtl2s3255:3251:10476:1 gene:EMLSAG00000006002 transcript:EMLSAT00000006002 description:"augustus_masked-LSalAtl2s3255-processed-gene-0.1")

HSP 1 Score: 60.077 bits (144), Expect = 4.676e-9
Identity = 69/283 (24.38%), Postives = 117/283 (41.34%), Query Frame = 0
Query:  805 MRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLESHARPSTLVMDVTSEANSQGIIVA-----------VDRIFTGSKNLDGNAIVHFFNALCDVSKE--EIGS-KQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFH---------FQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVT 1064
            + SKN++ ++ ++SVAH  GN L  SW  I+  +  L +  +LG       G  S   P+        E +S  I  A           V R+F  S +L   ++ H   ALC +S+E  E+ +  +   ++++ KL+E +  N+ R+ +    IW  + NH  LV C+         +      +L FL K  LQ  +          Q   L P  + +     P +R   +  +  +I +  + +K GW  I    G       + ++  S Q  Q I T
Sbjct:  348 LTSKNLQCMRAILSVAHCHGNLLGTSWHIILTSLQHLVW--ILGLKPVANGGALSSTSPTNSSTSTNVENSSSVITTAVMAXLPVLSNMVTRLFESSCDLSDESVNHLIEALCVLSEESMELATLNREPSLFAVAKLLETALVNLTRLDV----IWRPITNHLLLV-CQHPH----LRMRQWGSEALTFLCKQSLQQDYDPPINDSSKLQTLLLSPL-FELCAIPFPDVRQKQLDTVLHIIHTSGEALKDGWPVILNIIGSLDESHSDILIRNSFQCIQLIFT 618          
BLAST of GF14256 vs. SwissProt
Match: gi|408407575|sp|A2A5R2.1|BIG2_MOUSE (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Short=Brefeldin A-inhibited GEP 2; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 2)

HSP 1 Score: 1087.02 bits (2810), Expect = 0.000e+0
Identity = 575/1133 (50.75%), Postives = 753/1133 (66.46%), Query Frame = 0
Query:  228 DLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP-HQAE--KDAVPE---------------------------TPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGT-----SLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SGHGPD---GLESHA---------------------RPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            D+  H VA  F       +++Q+DA+LVFRSLCKLSMKPL EG PDPKSHELRSK++SL+LLLS+LQN+G  F+ +++FV+AIK YLCVALS+NGVS+V +VFELSLA+F++LL+ +++HLK QIE+FFKEI LNILE S+SSFE +WMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+  +E+G TP Q + +R + LECL  ILKCMVEWSK+LYVNP HQA   ++ +P+                           T  A  +DP   E +KQ+K ++E+GI+LF  KP +GI++L E  ++    +++AQFLH+E  RLD T +GEFLG+    NKE MY YVD+++F E +FVSALR FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IYD I   +I MK T     +  + K  +   KQR LL+N E E +++TA ALME+ S     FTSA  LDHV+PMFKL+W+PLLA +S+GLQ+ DD  V  LCLEGIRC +R+A IF   LERDA++QALARF+LLT +S++ EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG G +    L+ H+                     R      +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIWHV+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMV+RC++QM+ SQ+ NI+SGWKNIFA F  AA D   +IV L+ QTT  IV+ +   +  A  +SFQD + C+     N +  D    ++ ++      V +  +  +E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF++FYE L  V+L+D+ +Q QWC++Q NEQL++S  +C + LV +N  K     +    + + DI K T
Sbjct:  363 DVQGHQVAARF------SHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQE-ERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTT 1488          

HSP 2 Score: 186.422 bits (472), Expect = 3.842e-46
Identity = 98/203 (48.28%), Postives = 141/203 (69.46%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEP--GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMES 201
            MF+ +A+E +L +K+VKRPQ+ +LR AC+VAL +++A       + +   L    P     I  ++   PFELAC QSK+ ++V  SLD LQKL+AYGH+       P +  PG   L DR+V ++ + F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+
Sbjct:    9 MFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKA-------ELEKQRLGAAAPPKANFIEADKYFLPFELAC-QSKSPRVVSTSLDCLQKLIAYGHIT---GNAPDSGAPGK-RLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMEN 199          
BLAST of GF14256 vs. SwissProt
Match: gi|81865497|sp|Q7TSU1.1|BIG2_RAT (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Short=Brefeldin A-inhibited GEP 2; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 2)

HSP 1 Score: 1082.01 bits (2797), Expect = 0.000e+0
Identity = 573/1133 (50.57%), Postives = 752/1133 (66.37%), Query Frame = 0
Query:  228 DLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP-HQAE--KDAVPE---------------------------TPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGT-----SLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SGHGPD---GLESHA---------------------RPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            D   H VA  F       +++Q+DA+LVFRSLCKLSMKPL EG PDPKSHELRSK++SL+LLLS+LQN+G  F+ +++FV+AIK YLCVALS+NGVS+V +VFELSLA+F++LL+ +++HLK QIE+FFKEI LNILE S+SSFE +WMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+  +E+G TP Q + +R + LECL  ILKCMVEWSK+LYVNP HQA   ++ +P+                           T  A  +DP   E +KQ+K ++E+GI+LF  KP +GI++L E  ++    +++AQFLH+E  RLD T +GEFLG+    NKE MY YVD+++F E +FVSALR FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ I   +I MK T     +  + K  +   KQR LL+N E E +++TA ALME+ S     FTSA  LDHV+PMFKL+W+PLLA +S+GLQ+ DD  V  LCLEGIRC +R+A IF   LERDA++QALARF+LLT +S++ EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG G +    L+ H+                     R      +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIWHV+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMV+RC++QM+ SQ+ NI+SGWKNIFA F  AA D   +IV L+ QTT  IV+ +   +  A  +SFQD + C+     N +  D    ++ ++      V +  +  +E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF++FYE L  V+L+D+ +Q QWC++Q NEQL++S  +C + LV +N  K     +    + + DI + T
Sbjct:  363 DAQGHPVAARF------SHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVSSGTQTAIPDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQE-ERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTMATKSTKQNVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFRTT 1488          

HSP 2 Score: 187.193 bits (474), Expect = 2.619e-46
Identity = 99/203 (48.77%), Postives = 141/203 (69.46%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEP--GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMES 201
            MF+ +A+E +L +K+VKRPQ+ +LR AC+VAL +++A       + +   L    P     I  ++   PFELAC QSK+ ++V  SLD LQKL+AYGH+       P +  PG   L DR+V +V + F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+
Sbjct:    9 MFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKA-------ELEKQRLGAAAPPKANFIEADKYFLPFELAC-QSKSPRVVSTSLDCLQKLIAYGHIT---GNAPDSGAPGK-RLIDRIVETVCNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMEN 199          
BLAST of GF14256 vs. SwissProt
Match: gi|146329988|sp|Q9Y6D5.3|BIG2_HUMAN (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Short=Brefeldin A-inhibited GEP 2; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 2)

HSP 1 Score: 1078.16 bits (2787), Expect = 0.000e+0
Identity = 572/1133 (50.49%), Postives = 745/1133 (65.75%), Query Frame = 0
Query:  228 DLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP-HQA-----------------------------EKDAVPETPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGT-----SLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SGHGPD---GLESHA---------------------RPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDL--SLRDHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            D   H VA  F       +++Q+DA+LVFRSLCKLSMKPL EG PDPKSHELRSK++SL+LLLS+LQN+G  F+ +++F++AIK YLCVALS+NGVS+V +VFELSLA+F++LL+ +++HLK QIE+FFKEI LNILE S+SSFE +WMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+  +E+G TP Q + +R + LECL  ILKCMVEWSK+LYVNP HQ                              E      T     +DP   E +KQ+K ++E+GI+LF  KP +GI++L E  ++    +++AQFLH+E  RLD T +G+FLG+    NKE MY YVD+++F E +FVSALR FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ I   +I MK T     +  + K  +   KQR LL+N E E +++TA ALME+ S     FTSA  LDHV+PMFKL+W+PLLA +S+GLQ+ DD  V  LCLEGIRC IR+A IF   LERDA++QALARF+LLT +S++ EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG G +    L+ H                      R      +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIWHV+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDM +RC++QM+ SQ+ NI+SGWKNIFA F  AA D   +IV L+ QTT  IVT +   +  A  +SFQD + C+     N +  D    ++ ++      V +  +  +E              W R  + +LF +                   FE+ K                     FD   L   L +  EW+ TTCNHALYA+ DVF++FYE L  V+L+D+ +Q QWC++Q NEQL++S  +C + LV +N  K   + +    + + DI K T
Sbjct:  356 DAQGHQVAARF------SHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQE-ERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTT 1481          

HSP 2 Score: 185.652 bits (470), Expect = 7.432e-46
Identity = 98/203 (48.28%), Postives = 140/203 (68.97%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEP--GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMES 201
            MF+ +A+E +L +K+VKRPQ+ +LR AC+VAL +++A       + +   L    P     I  ++   PFELAC QSK+ ++V  SLD LQKL+AYGH+       P +  PG   L DR+V ++   F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+
Sbjct:    9 MFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKA-------EIEKQRLGTAAPPKANFIEADKYFLPFELAC-QSKSPRVVSTSLDCLQKLIAYGHIT---GNAPDSGAPGK-RLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMEN 199          
BLAST of GF14256 vs. SwissProt
Match: gi|13123969|sp|O46382.1|BIG1_BOVIN (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Short=Brefeldin A-inhibited GEP 1; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 1; AltName: Full=p200 ARF guanine nucleotide exchange factor; AltName: Full=p200 ARF-GEP1)

HSP 1 Score: 1055.43 bits (2728), Expect = 0.000e+0
Identity = 574/1121 (51.20%), Postives = 739/1121 (65.92%), Query Frame = 0
Query:  244 YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQA-----EKDAVPETPE-------------------------------ATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTS-----LANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SG--HGPDGLESHAR---PSTLV-----------------MDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFDIVK---QHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            + +++Q+DA+LVFRSLCKLSMKPL +G PDPKSHELRSKILSL+LLLSILQN+G  F  N++F++AIK YLCVALS+NGVS+V EVFELSL++F++LL+ ++ HLK QIE+FFKEI L ILE S+SSF+ KWMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+ + E+G +  Q + +R + LECL  ILKCMVEWSK+ YVNP+       EK +  ET E                               +  ++P   E +KQ+K ++E GI LFT KP +GI+YL E  ++    +++AQFLH+E  RLD T +GEFLG+ +  NKE MY YVD+ +F+  DFVSALR+FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ IA  +I MK T        ++K  +   KQR LL+N E E +++TA ALME+ S     FTSA  L+HV+PMFKL W+P LA FSVGLQ  DD  V  LCLEGIRC IR+A IF+  LERDA++QALARFTLLT +S + EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG   G +G  + A+   P   V                  +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIW V+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAA D  ESIV L+ QTT  IVT +   +  A  +SFQD + C+     N +  D    ++ ++    K      Q+F+E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF+++ EVL  V+L D+ +Q  WC+QQ NEQL++S  +C + +V  N  K   + +    +   DI K T
Sbjct:  415 FSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFGTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQETSEMKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFTKKPKRGIQYLQEQGMLGTTPEDIAQFLHQE-ERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPAKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGAKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGYTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTT 1534          

HSP 2 Score: 189.119 bits (479), Expect = 7.023e-47
Identity = 102/229 (44.54%), Postives = 149/229 (65.07%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA-----LPASNTSDGQCSVLPEIEPG-QLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPETASEAVHVVR 223
            MFL +A+E +L +K+VK+  + +LR+ACEVAL +++A      P    +    S LP ++     I  ++   PFELAC QSK  ++V  SLD LQKL+AYGHL       P +  PG   L DR++ ++   F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+ +       E E   +  H+++
Sbjct:   10 MFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELAC-QSKCPRIVSTSLDCLQKLIAYGHLT---GNAPDSTTPGK-KLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQ 233          

HSP 3 Score: 59.6918 bits (143), Expect = 2.199e-7
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 0
Query: 1318 LVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            L+KQET +  C L IL  + T        + +++  ++     L YFL++T+++ +E W  +L     ++L   +  F +  S  Y L+C+I+   +  E R VL +   RIG ++ I
Sbjct: 1719 LLKQETSSLACGLRILFRMYTDESRASAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQI 1836          
BLAST of GF14256 vs. SwissProt
Match: gi|408387574|sp|G3X9K3.1|BIG1_MOUSE (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Short=BIG1; Short=Brefeldin A-inhibited GEP 1; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 1)

HSP 1 Score: 1052.74 bits (2721), Expect = 0.000e+0
Identity = 570/1121 (50.85%), Postives = 734/1121 (65.48%), Query Frame = 0
Query:  244 YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQA-----EKDAVPETPE-------------------------------ATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTS-----LANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SG--------------HGPD--------GLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFDIVK---QHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            + +++Q+DA+LVFRSLCKLSMKPL +G PDPKSHELRSKILSL+LLLSILQN+G  F+ N++F++AIK YLCVALS+NGVS+V EVFELSL++F++LL+ ++ HLK QIE+FFKEI L ILE S+SSF+ KWMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+ + E+G +  Q + +R + LECL  ILKCMVEWSK+ YVNP+       EK +  E  E                               +  ++P   E +KQ+K ++E GI LF  KP +GI+YL E  ++    +++AQFLH+E  RLD T +GEFLG+ +  NKE MY YVD+ +F+  DFVSALR+FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ IA  +I MK T        + K  +   KQR LL+N E E +++TA ALME+ S     FTSA  L+HV+PMFKL W+P LA FSVGLQ  DD  V  LCLEGIRC IR+A IF+  LERDA++QALARFTLLT +S + EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG                PD        G     +    + +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIW V+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAA D  ESIV L+ QTT  IVT +   +  A  +SFQD + C+     N +  D    ++ ++    K      Q+F+E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF+++ EVL  V+L D+ +Q  WC+QQ NEQL++S  +C + +V  N  K   + +    +   DI K T
Sbjct:  412 FSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEVKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQE-ERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMSVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTT 1531          

HSP 2 Score: 189.889 bits (481), Expect = 3.235e-47
Identity = 101/229 (44.10%), Postives = 148/229 (64.63%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLR------ALPASNTSDGQCSVLPEIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPETASEAVHVVR 223
            MFL +A+E +L +K+VK+  + +LR+ACEVAL +++      + P      G  ++ P       I  ++   PFELAC QSK  ++V  SLD LQKL+AYGHL     R P +  PG   L DR++ ++   F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+ +       E E   +  H+++
Sbjct:   10 MFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELAC-QSKCPRIVSTSLDCLQKLIAYGHLT---GRAPDSTTPGK-KLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQQHLLQ 233          
BLAST of GF14256 vs. SwissProt
Match: gi|116241267|sp|Q9Y6D6.2|BIG1_HUMAN (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Short=Brefeldin A-inhibited GEP 1; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 1; AltName: Full=p200 ARF guanine nucleotide exchange factor; AltName: Full=p200 ARF-GEP1)

HSP 1 Score: 1050.81 bits (2716), Expect = 0.000e+0
Identity = 570/1121 (50.85%), Postives = 735/1121 (65.57%), Query Frame = 0
Query:  244 YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQA-----EKDAVPETPE-------------------------------ATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTS-----LANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SG--------------HGPD--------GLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFDIVK---QHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            + +++Q+DA+LVFRSLCKLSMKPL +G PDPKSHELRSKILSL+LLLSILQN+G  F+ N++F++AIK YLCVALS+NGVS+V EVFELSL++F++LL+ ++ HLK QIE+FFKEI L ILE S+SSF+ KWMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+ + E+G +  Q + +R + LECL  ILKCMVEWSK+ YVNP+       EK +  E  E                               +  ++P   E +KQ+K ++E GI LF  KP +GI+YL E  ++    +++AQFLH+E  RLD T +GEFLG+ +  NKE MY YVD+ +F+  DFVSALR+FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ IA  +I MK T        ++K  +   KQR LL+N E E +++TA ALME+ S     FTSA  L+HV+PMFKL W+P LA FSVGLQ  DD  V  LCLEGIRC IR+A IF+  LERDA++QALARFTLLT +S + EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG                PD        G     +    + +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIW V+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAA D  ESIV L+ QTT  IVT +   +  A  +SFQD + C+     N +  D    ++ ++    K      Q+F+E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF+++ EVL  V+L D+ +Q  WC+QQ NEQL++S  +C + +V  N  K   + +    +   DI K T
Sbjct:  415 FSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQE-ERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTT 1534          

HSP 2 Score: 188.734 bits (478), Expect = 7.266e-47
Identity = 102/229 (44.54%), Postives = 149/229 (65.07%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA-----LPASNTSDGQCSVLPEIEPG-QLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPETASEAVHVVR 223
            MFL +A+E +L +K+VK+  + +LR+ACEVAL +++A      P    +    S LP ++     I  ++   PFELAC QSK  ++V  SLD LQKL+AYGHL       P +  PG   L DR++ ++   F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+ +       E E   +  H+++
Sbjct:   10 MFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELAC-QSKCPRIVSTSLDCLQKLIAYGHLT---GNAPDSTTPGK-KLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQ 233          
BLAST of GF14256 vs. SwissProt
Match: gi|408407574|sp|D4A631.1|BIG1_RAT (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Short=BIG1; Short=Brefeldin A-inhibited GEP 1; AltName: Full=ADP-ribosylation factor guanine nucleotide-exchange factor 1)

HSP 1 Score: 1048.88 bits (2711), Expect = 0.000e+0
Identity = 569/1121 (50.76%), Postives = 733/1121 (65.39%), Query Frame = 0
Query:  244 YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQA-----EKDAVPETPE-------------------------------ATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTS-----LANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGS-------SG--------------HGPD--------GLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFDIVK---QHGQSFEES-------------WWRDLYQVLFRI-------------------FEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            + +++Q+DA+LVFRSLCKLSMKPL +G PDPKSHELRSKILSL+LLLSILQN+G  F+ N++F++AIK YLCVALS+NGVS+V EVFELSL++F++LL+ ++ HLK QIE+FFKEI L ILE S+SSF+ KWMV+Q +  IC DAQ V+DI+VNYDCDL AANIFERLV+DL+K+AQG+ + E+G +  Q + +R + LECL  ILKCMVEWSK+ YVNP+       EK +  E  E                               +  ++P   E +KQ+K ++E GI LF  KP +GI+YL E  ++    +++AQFLH+E  RLD T  GEFLG+ +  NKE MY YVD+ +F+  DFVSALR+FL  FRLPGEAQKIDRLMEKFA+RY+ECN  Q LF SAD AYVLAYS+IMLTTDLHS  VK KMTKE++++ N+GINDS+D+P EYLS IY+ IA  +I MK T        + K  +   KQR LL+N E E +++TA ALME+ S     FTSA  L+HV+PMFKL W+P LA FSVGLQ  DD  V  LCLEGIRC IR+A IF+  LERDA++QALARFTLLT +S + EM+ KNI+ IKTLI+VAHTDGNYL +SW +I+KCISQLE AQL+G+       SG                PD        G     +    + +   E +SQ ++VAVDRIFTGS  LDGNAIV F   LC VS +E+ S    RM+SL K+VEISYYNM RIRL+WSRIW V+G+HFN VGC  +++V+ FAVDSL+QLS+KFLEKGEL NF FQKDFLRPFE+IMK N+SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAA D  ESIV L+ QT+  IVT +   +  A  +SFQD + C+     N +  D    ++ ++    K      Q+F+E              W R  + +LF +                   FE+ K                     FD   L  +  +  EW+ TTCNHALYA+ DVF+++ EVL  V+L D+ +Q  WC+QQ NEQL++S  +C + +V  N  K   + +    +   DI K T
Sbjct:  412 FSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQE-ERLDSTQAGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTSGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCVINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTT 1531          

HSP 2 Score: 189.504 bits (480), Expect = 4.469e-47
Identity = 109/285 (38.25%), Postives = 165/285 (57.89%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLR------ALPASNTSDGQCSVLPEIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPETASEAVHVVRPILLDLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHEL 279
            MFL +A+E +L +K+VK+  + +LR+ACEVAL +++      + P      G  ++ P       I  ++   PFELAC QSK  ++V  SLD LQKL+AYGHL  S    P +  PG   L DR++ ++   F GPQTDE VQLQ++KALLT +TS ++ +H+ ++L +VRTCYNIYLAS+N+INQTTAKATL+Q+LN +F+ ME+ +       E E   +  H+++  +           +L P+   ++ Q                   EG  DP++H++
Sbjct:   10 MFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELAC-QSKCPRIVSTSLDCLQKLIAYGHLTGS---APDSTTPGK-KLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQHHLLQSPVSHHEPESPHLRYLPPQTVDHIAQEQ-----------------EGDLDPQTHDV 272          

HSP 3 Score: 58.5362 bits (140), Expect = 4.243e-7
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 0
Query: 1318 LVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            L+KQET +  C L IL  + T        + +++  ++     L YFL++T+++ +E W  +L     ++L   +  F +  S  Y L+C+I+   +  E R VL +   RIG ++ I
Sbjct: 1716 LLKQETSSLACGLRILFRMYTDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGIVFQI 1833          
BLAST of GF14256 vs. SwissProt
Match: gi|449061809|sp|F4JSZ5.1|BIG1_ARATH (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1; Short=BIG1; AltName: Full=ARF guanine-nucleotide exchange factor BIG1)

HSP 1 Score: 637.106 bits (1642), Expect = 0.000e+0
Identity = 388/1073 (36.16%), Postives = 585/1073 (54.52%), Query Frame = 0
Query:  134 DEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASA-VSLPS----------HEPETASEAVHVVRPILLDLADHLVAREFLAP------------------EVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILE-ASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQ-AYEIGATPNQLMQMRIRCLECLCFILKCMVEW-SKELYVN----PHQAEKDAVPETPEATDE-------------------DPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELEN--KEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTSLAN--------NKVIITDLKQRTLLWNRESESISQTAGALME-------SASSRTD-AFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHG------------------PDGLESHARPSTLVMDV---------TSEANSQGII--------------------VAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCVI 1086
            +E ++L VL+ LL  + S  + +  + LL  VRTCYN+YL   N  NQ  AK+ L+QI+  VF+  E+ S   SL +           +      +VH+ +  + D+   + A E   P                  E   + ++ D +L+F++LCKLSMK   +   D +   +R K LSLELL  I+ N G  +  ++ F++AIK  LC++L +N   +V  +F+L  A+F +LL KYR  +K ++ IFF  + L +LE     SF QK  V+  + NICHD  ++IDIFVN+DCD+E+ NIFER+V+ L K A G         +P Q +  R   ++CL  I+K M  W  ++L V     P   E +A       ++E                   D A LE+ +  K   + G+ LF  KP KGI++L+ +  V     EV  FL +    L+ T IG++LGE E+   + M+ YVD  +F E++F  A+R FL  FRLPGEAQKIDR+MEKFA R+ +CNP    F SAD AYVLAYSVIML TD H+  VK+KMTK +F+RNN+GI+D +D+P EYL  +YD +  NEIKM   S A         NK++  D     + W +  E      G L++       S S +++ A+     +  ++ M ++ W P+LA FSV L  +DD +    CL G R  + +  +     +RDAF+ ++A+FT L       +M+ KN++A+K +IS+A  DGN+LQ +W  I+ C+S++E+ QLLG                       P+  +  A  + ++M V         T   N  G++                      ++ ++  S+ L   AIV F  ALC VS  E+ S    R++SL KLVEI++YNM RIRL WSRIW ++ + F  VG  ++ +V+ F +DSL+QLS+KFLE+ EL N++FQ +FLRPF  +M+ + S +IR+++VRC+SQM+ S+  N+KSGWK++F  F  AA D+ ++IV L+ +T ++IV +  S        +F DC+ C+I
Sbjct:  131 EESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGFNGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLAITDKNVNEGNSVHICQGFINDV---ITAGEAAPPPDFALVQPPEEGASSTEDEGTGSKIREDGFLLFKNLCKLSMKFSSQENTDDQIL-VRGKTLSLELLKVIIDNGGPIWLSDERFLNAIKQLLCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLRVLENVLQPSFVQKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALGPPPGSSTILSPVQDITFRHESVKCLVSIIKAMGTWMDQQLSVGDSLLPKSLENEAPANNHSNSNEEDGTTIDHDFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEFLISSKKVGNSPDEVVSFL-RNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGEAIRFFLRGFRLPGEAQKIDRIMEKFAERFCKCNPNS--FSSADTAYVLAYSVIMLNTDAHNIMVKEKMTKADFIRNNRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNKLLGLDGILNLVYWTQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVEVSWGPMLAAFSVTLDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNL---HCAGDMKQKNVDAVKAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFASTETEEKKALGFPNLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSFQLNNVYAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLVEIAHYNMNRIRLVWSRIWSILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYNFQNEFLRPFVIVMQKSSSAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADERKNIVLLAFETMEKIVREYFSYITETEATTFTDCVRCLI 1193          
BLAST of GF14256 vs. SwissProt
Match: gi|449061867|sp|F4IXW2.2|BIG5_ARATH (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 5; Short=BIG5; AltName: Full=ARF guanine-nucleotide exchange factor BIG5; AltName: Full=Protein BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE 1; Short=Protein BEN1; AltName: Full=Protein HOPM INTERACTOR 7)

HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 408/1138 (35.85%), Postives = 616/1138 (54.13%), Query Frame = 0
Query:   63 ERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDR--------LVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTME----SASAV-----------SLPSHEPETAS------------------------EAVHVV--------RPILLDLADHLVAREFLAPEVYRNMM---QRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIG-ATPNQLMQMRIRCLECLCFILKCMVEWSK---ELYVNPHQAEKDAV----PETPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELEN--KEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPP-EYLSKIYDAIASNEIKMK-------------GTSLANNKVIITDLKQRTLL----WNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLESHARPSTLVMDVTSEANSQGIIVAV--------DRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQS-VRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCVI----NKSKLDIRTRSLTIL 1101
            E +L P  LA  ++KN K+   +LD L KL+AY HL             G P L+          +++ V         D  V LQVLK LLT + S   +VH   LL  +R CYNI L S++ INQ T+KA L+Q+++ VF  ME    SAS+            S P +E  TA+                        E +H +            LD A HL   + +   +    M   QRDA LVFR+LCK+ MK       D      +++ILSLELL  +L+    +F  N  F+ ++K+YL  AL +  VS  S +F+ +  +F  LL ++R  LK +I IFF  I L  L+ S    +QK  V++ +  +C D Q+++D++VNYDCDLEA N+FER+V  L+K+AQG Q+ +   A  +Q   ++   L+CL  +LK +V+W K   E   +   A +D+     P   ++ ++ P++ E+ K  K  +E  I  F    VKG++YL+ N +V +    VAQFL +  + L K  IG++LG+ E      M+ YVD + F+E+ F SA+R FL  FRLPGEAQKIDR+MEKFA RY   NP   LFK+AD AYVLAY+VIML TD H+  V  KM+K +F R N   ND ED  P E L +IYD+I   EIK+K             G       V I +L     +       E+E I +    +      +   F + +++D ++PM + +  PLLA FSV ++  D+   + LC+EG +  I +A +      R AF+ +L RFT L       EMRSKN+EA++ L+ +  ++ + LQ +W  +++C+S+LE+  ++ + G            +  VM  +++ +  G++ ++        +++F  S  L   ++V FF ALC VS EE+  KQS  R++SL KLVEISYYN+ RIR+ W+RIW V+  HF   G   D+ ++ +A+DSL+QL +K+LE+ EL NF FQ D L+PF  IM+N +S  IR ++V C+ QMI+S+  +IKSGW+++F  F  AA D+VESIV  S +  ++++ +        + + F DC++C+I    NK+   I  +++ +L
Sbjct:  105 ELVLKPLRLAF-ETKNLKIFDAALDCLHKLIAYDHLE------------GDPGLDGGKNSAPFTDILNMVCSCVDNSSPDSTV-LQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLGDALTQAKDTTLASVEELHTLVGGADIKGLEAALDKAVHLEDGKKIKRGIELESMSIGQRDALLVFRTLCKMGMKE------DSDEVTTKTRILSLELLQGMLEGVSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYASGIFSVLLLRFRDSLKGEIGIFFPIIVLRSLDNSECPNDQKMGVLRMLEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKSREDVPSNFEKAKAHKSTMEAAISEFNRNSVKGVEYLIANKLVERNPASVAQFL-RSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSMKFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNP--GLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMN-ATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKLSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFRKHGVKRGVFHTVEQVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFLTSLVRFTFL---HAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEF--IISTPGI-----------AATVMHGSNQISRDGVVQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEEL--KQSPARVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLGMKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSVFMIFTAAADDEVESIVEKSFENVEQVILEHFDQ---VIGDCFMDCVNCLIRFANNKASDRISLKAIALL 1197          
BLAST of GF14256 vs. SwissProt
Match: gi|75264111|sp|Q9LPC5.1|BIG3_ARATH (RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 3; Short=BIG3; AltName: Full=ARF guanine-nucleotide exchange factor BIG3; AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 10)

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 360/961 (37.46%), Postives = 566/961 (58.90%), Query Frame = 0
Query:  228 DLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILE-ASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGAT-PNQLMQMRIRCLECLCFILKCMVEW-SKELYV-NPHQA----------EKDAVP-------------ETPEATDE------DPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENK--EAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTSLA--------NNKVIITDLKQRTLLWNRESESISQTAGALM--------ESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPD-----------GLESHARPSTLVM--------------------------------DVTSEANSQGI--------IVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCVI 1086
            +LAD  V ++  +     N ++RDA+LVFR+LCKLSMK  P+  P+     +R KI++LELL  +L+N+G  F+ +D F+ AIK YLC++L +N  SN+  +F+LS ++ +SL++++R  LK +I +FF  I L +LE  +   F+QK +V++ +  +C D+QI++DIF+NYDCD+ ++NIFER+V+ L K AQG     +    P Q   M++  ++CL  +L+ M +W +K+L + +P+ A          E+ + P             E  ++  E      D   +E+ +  K  L+ GI +F  KP KGI++L++ + V    +E+A FL K+ + L+KT IG++LGE E+   + M+ YVD   F  ++F  A+R FL  FRLPGEAQKIDR+MEKFA R+ +CNP  K F SAD AYVLAYSVI+L TD H+  VK KMT + F+RNN+GI+D +D+P EYL  +Y+ I+ NEIKMK   L         +++++  D     ++  R  +   +T+  L+        E A      + +A  +  ++ M ++ W+P+LA FSV L  +DD V+  LCLEG    I + ++ +    RDAF+ +LA+FT L   +++   + KNIEAIK ++ +A  +GNYLQ +W  I+ C+S+ E+  LLG  G  PD           G    A+P+++                                   VTSE  +  I        +  + RIFT S+ L+  AI+ F  ALC VS +E+ S    R++SL K+VEI++YNM RIRL WS IWHV+ + F  +GC D+ +++ FA+DSL+QLS+KFLE+ EL N++FQ +F++PF  +M+ + + +IR++++RC+SQM+ S+  N+KSGWK++F  F  AA D  ++IV LS +  ++I+             +F DC++C++
Sbjct:  316 ELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMKTPPKEDPE----LMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASNLMIIFQLSCSILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLRSMGDWVNKQLRLPDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGNSDALAIEQRRAYKLELQEGISIFNQKPKKGIEFLIKANKVGDSPEEIAAFL-KDASGLNKTLIGDYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAERFCKCNP--KDFSSADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEEYLRALYERISRNEIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMETSDDLIRHMQERFKEKARKSESVYYAASDVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLCLEGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSPADI---KQKNIEAIKAIVKLAEEEGNYLQDAWEHILTCVSRFEHLHLLG-EGAPPDATFFAFPQTESGNSPLAKPNSVPAIKERAPGKLQYAASAMIRGSYDGSGVAGKASNTVTSEQMNNLISNLNLLEQVGDMSRIFTRSQRLNSEAIIDFVKALCKVSMDELRSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSDNLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDNVKSGWKSMFMIFTTAAHDAHKNIVFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLV 1265          

HSP 2 Score: 78.9518 bits (193), Expect = 2.644e-13
Identity = 67/197 (34.01%), Postives = 105/197 (53.30%), Query Frame = 0
Query:   20 QYQELREACEVALADLRAL----PASNTSDGQCSVLPEIEPG----QLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNP-GHPS--LEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAV 205
            ++ +L   C+  +  LR+     P +++  G     P  + G     L   E IL P  +    +   K+V  ++D +QKL+A+G++R          +P G P   L  +L+ ++         DE ++L VLK LLT +TS ++R+H +SLL  VRTCY IYL SRN++NQ TAKA+L Q+   VF  ME+ S+ 
Sbjct:   29 KHSKLAHECKSVIERLRSPENSSPVADSESGSSIPGPLHDGGAAEYSLAESEIILSPL-INASSTGVLKIVDPAVDCIQKLIAHGYVRG-------EADPTGGPEALLLSKLIETICKCHE--LDDEGLELLVLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYLGSRNVVNQATAKASLVQMSVIVFRRMEADSST 215          

HSP 3 Score: 58.151 bits (139), Expect = 5.948e-7
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 0
Query: 1090 KLDIRTRSLTILFDIVKQHGQSFEESWWRDLYQ-VLFRIFEVGKFDMN---DLSLR--------DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLV 1204
            + +IR  +L +LFD ++ HG  F  + W  +++ VLFRIF+  + D++   D S          D   W+  TC+ AL  + D+F  FY+  V  +L  +L  +   I++P++ L+ + I+    L+
Sbjct: 1346 RAEIRKVALKVLFDTLRNHGDHFSLALWERVFESVLFRIFDYVRQDVDPSEDDSTDQRGYNGEVDQESWLYETCSLALQLVVDLFVNFYKT-VNPLLKKVLMLFVSLIKRPHQSLAGAGIAALVRLM 1471          
BLAST of GF14256 vs. nr
Match: gi|1012973208|gb|KYQ47635.1| (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Trachymyrmex zeteki])

HSP 1 Score: 1276.92 bits (3303), Expect = 0.000e+0
Identity = 728/1569 (46.40%), Postives = 983/1569 (62.65%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA----LPASNTSDGQCSVLPEIEP-GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESAS--------------AVSLPSHEPETASEAVHVVRPILL------DLADHLVAR-EFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAE--------KDAVPETP--------------------EATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMK---GTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLESHARP---------STLVMDVTS--------------------EANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDN------KFALKESFQDC--LSCVINKSKLDIRTRSLTILFDIVKQHGQSFEESWWRDLYQVLFRIFEVGKFDMNDLSLRDHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT---SVIVYKEE--------DIVFGRVH-VHLDVLEVS----------------------MNVLTSTAYGQTIEGPPWFEVLMPDH-FQVLKKLLRS--LEQSFN---ESKSVLEDTPNSSRKRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L ++DVKR    +LR++CE AL DLR     +PA    +   + LP+ +    +I  E+   PFELAC QSK+ ++V+ +LD LQKL+AYGHL      +P +  P    L  R+V ++   F+GPQTDE VQLQ++KALLT++TS +V VH+ ++LL++RT YN+YLASRN++NQTTA+ATL+Q++N +F+ ME+ +                 + + E ET   A +V  P+ L      D  D   A  + +    + +++Q+DA+LVFR+LCKLSMKPLP+G PDPKSHELRSKILSL+LLL ILQN+G   + N++FV AIK YLCVALS+NGVS+V EVFELSLALF++LL ++++HLK QIE+FFKEI +NILE SSSSFE KWMV+  +  IC DAQ           DL AAN+FERLV+DL+K+AQG+QA E+GA+PNQ   MRIR LECL  ILKCMVEWS++LYVNP             D  PE P                    +   + P   E  KQ+K V E GI++F+ KP KG++YL E  ++    ++VA++LH +  RLDKTAIG+FLG+  + + MYHY+D++NFA+ D V+ALR FL  FRLPGEAQKIDRLMEKFASRY ECNP   LF SAD AY+L +S+IMLTTDLHS  VK KMTKE+++R N+  +D+ED+P EYLS+IYD IA NEIKMK     S    K +I+  K+R LLWN E E IS  A  LMES S     FT+AK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ SLERDA++QALARFTLLT NS + EM++KNI+ IKTLI+VAHTDGNYL  SWLD++KCISQLE AQL+G+ G  P  L   ++P          +L   V S                    E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS EE+      RM+SL K+VEISYYNM RIRL+WSRIW V+G+HF+ VGC   Q+++FFAVDSL+QL+ KF+EKGE  NF FQKDFLRPFE+IMK N+SP IRDMVVRC++Q++ SQ+ NI+SGWKNIF+ F  AA D  ES+V L+   T +I+  L+  +        +++  F     LSCV+++ KLD+RTR+LT+LFD+VK HG SF+  WW+DL+QVLFRIF+  K         +  EW+ TTCNHALYA+ DVFS+FY+ L  ++L  L  Q  WC+Q+ NEQL++S  +C + LV +N  K +  T+      + DI + T   +++ +K          D++ G     H+ +L+ S                      M ++      Q  E    +  L  +H  Q+++ LLRS    +SFN   E ++VL     S R      L+KQET++  C L IL ++                               + +  +  W ++ A + E      +  F    S+ Y  +C+++   +  E R +L +   RIG ++ I
Sbjct:   11 MFIVRALEKILADRDVKRSHLSQLRKSCESALEDLRNEIKDVPAVQGEEVTSNALPQPKSDSNVITAEKYFLPFELAC-QSKSPRIVVTALDCLQKLIAYGHLT---GNVPDSTEPNK-LLIVRIVETICGCFTGPQTDEGVQLQIIKALLTVMTSQHVEVHEGTVLLTIRTVYNVYLASRNLVNQTTARATLTQMINVIFARMETQAEEDNIRIDGEHQQEVPVIVNGETETELSAENV--PVSLENGSPEDNGDEAAAENDNMVTAKFTHVLQKDAFLVFRALCKLSMKPLPDGTPDPKSHELRSKILSLQLLLGILQNAGPVLRSNEMFVIAIKQYLCVALSKNGVSSVPEVFELSLALFLALLARFKMHLKMQIEVFFKEIFMNILETSSSSFEHKWMVIHALTRICADAQ----------SDLSAANLFERLVNDLSKIAQGRQALELGASPNQEKSMRIRGLECLVSILKCMVEWSRDLYVNPSVPADQQPLSDLPDTAPEIPLPRYGSAGSLSSANSSLIGNKEVPDSPEQYEVQKQQKEVWETGIEIFSRKPGKGVQYLQEQGLLGTSQEDVARWLHLD-ERLDKTAIGDFLGDHNHNQVMYHYIDQMNFADRDLVTALRYFLEGFRLPGEAQKIDRLMEKFASRYCECNPNNGLFTSADTAYILGFSIIMLTTDLHSPQVKNKMTKEQYIRLNRRNSDNEDLPEEYLSRIYDEIAGNEIKMKSNPNNSRLAGKQLISSEKKRRLLWNMEMEVISTAAKNLMESVSHVQAPFTTAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLTANSPITEMKAKNIDTIKTLITVAHTDGNYLGSSWLDVVKCISQLELAQLIGT-GVRPQLLGPPSKPHFPSPLANFGSLAHSVNSHQTNNLNLSSLDPSVKESIGETSSQSVVVAVDRIFTGSTRLDGDAIVEFVKALCQVSLEELAHPTQPRMFSLTKIVEISYYNMGRIRLQWSRIWQVLGDHFDRVGCSPRQDIAFFAVDSLRQLATKFIEKGEFANFRFQKDFLRPFEHIMKKNRSPVIRDMVVRCVAQIVHSQAPNIRSGWKNIFSVFHHAASDRDESVVELAFSMTGKIINILLVRDFLLILYNLSVRGWFPLLFELSCVVSRCKLDVRTRALTVLFDVVKTHGASFKPHWWKDLFQVLFRIFDNMKLPEQHT---EKAEWMTTTCNHALYAIVDVFSQFYDTLGPLLLEQLYFQLLWCVQKDNEQLARSGTNCLENLVISNGIKFDEQTWQKTCQCVLDIFESTLPSALLTWKPHSPNKESDLDVITGESDGFHVGILKRSNSAQSLNTEILPKAKLFSASYAMQIVFRAGADQQKEEQGMYCALTTNHLLQLVECLLRSHRFAKSFNSNHEQRNVLWKA--SFRGNMKPNLLKQETQSLACALRILFKM------------------------------YSDEAHRTDWSKIEARLVE----MNDHRFAIHASTCYPCLCEVMCFDLKPELRSILRRFFLRIGPVFRI 1521          
BLAST of GF14256 vs. nr
Match: gi|1070611469|ref|XP_018403612.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 2 [Cyphomyrmex costatus])

HSP 1 Score: 1257.66 bits (3253), Expect = 0.000e+0
Identity = 731/1640 (44.57%), Postives = 993/1640 (60.55%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA----LPASNTSDGQCSVLPEIEP-GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMES----------------ASAVSLPSHEPETASEAVH---------VVRPILLDLADHLVARE----------------------FLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAE--------KDAVPETP--------------------EATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMK---GTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGH----GPDGLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV---------INKSKLDIR-TRSLTILFD---------IVKQHGQSFEE--SWWRDLYQVLFR-------------------IFEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT---SVIVYKE----------------------------------------EDIVFGRV------HVHLDVLEVSMNVLTSTAYGQTIEGPPWFEVLMPDH-FQVLKKLLRS--LEQSFN---ESKSVLEDTPNSSRKRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L ++DV+R    +LR++CE AL DLR     +PA    +   + LP+ +    +I  E+   PFELAC QSK+ ++V+ +LD LQKL+AYGHL      +P +  P    L  R+V ++   F+GPQTDE VQLQ++KALLT++TS +V VH+ ++LL++RT YN+YLASRN++NQTTA+ATL+Q++N +F+ ME+                 + +     E E +SE V          +VR IL D+   +V  E                       +    + +++Q+DA+LVFR+LCKLSMKPLP+G PDPKSHELRSKILSL+LLL ILQN+G   + N++FV AIK YLCVALS+NGVS+V EVFELSLALF++LL ++++HLK QIE+FFKEI +NILE SSSSFE KWMV+  +  IC DAQ V+DI+VNYDCDL AAN+FERLV+DL+K+AQG+QA E+GA+PNQ   MRIR LECL  ILKCMVEWS++LYVNP             D  PE P                    +   + P   E  KQ+K V E GI++F+ KP KG++YL E  ++    ++VA++LH +  RLDKTAIG+FLG+  + + MYHY+D++NFAE D V+ALR FL  FRLPGEAQKIDRLMEKFASRY ECNP   LF SAD AY+L +S+IMLTTDLHS  VK KMTKE+++R N+  +D+ED+P EYLS+IYD IA NEIKMK     S    K +I+  K+R LLWN E E IS  A  LMES S     FT+AK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ SLERDA++QALARFTLLT NS + EM++KNI+ IKTLI+VAHTDGNYL  SWLD++KCISQLE AQL+G+       GP    + +     V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS EE+      RM+SL K+VEISYYNM RIRL+WSRIW V+G+HF+ VGC   Q+++FFAVDSL+QL+ KF+EKGE  NF FQKDFLRPFE+IMK N+SP IRDMVVRC++Q++ SQ+ NI+SGWKNIF+ F  AA D  ES+V L+   T +I+ +L +++   + +SFQD + C+          + S   IR  RS     D         ++   G   EE  +W R  + +LF                    +F+V K                     FD   L  +  +  EW+ TTCNHALYA+ DVFS+FY++L  ++L  L  Q  WC+QQ NEQL++S  +C + LV +N  K +  T+      + DI + T   +++ +K                                         ++IVF           +L + +  M    S +  Q  E    +  L  +H  Q+++ LLRS    +SFN   E ++VL     S R      L+KQET++  C L IL ++ +          +E   V      L YFL++  +  ++ W  +L  +  +I+   +  F    S+ Y  +C+++   +  E R +L +   RIG ++ I
Sbjct:   11 MFIVRALEKILADRDVRRSHLSQLRKSCESALEDLRNEIKDVPAVQGEEVTSNALPQPKSDSNVITAEKYFLPFELAC-QSKSPRIVVTALDCLQKLIAYGHLT---GNVPDSTEPNK-LLIVRIVETICGCFTGPQTDEGVQLQIIKALLTVMTSQHVEVHEGTVLLTIRTVYNVYLASRNLVNQTTARATLTQMINVIFARMETQAEEDNIRIDGEHQQEVTVIVNGETETELSSENVPGSDSIDSQIIVRGILDDVVKSVVPLEEEVSLENGNPEDNGDEAVTENDNMVTAKFTHVLQKDAFLVFRALCKLSMKPLPDGTPDPKSHELRSKILSLQLLLGILQNAGPVLRSNEMFVIAIKQYLCVALSKNGVSSVPEVFELSLALFLALLARFKMHLKMQIEVFFKEIFMNILETSSSSFEHKWMVIHALTRICADAQSVVDIYVNYDCDLSAANLFERLVNDLSKIAQGRQALELGASPNQEKSMRIRGLECLVSILKCMVEWSRDLYVNPSVPADQQPLSDLPDTAPEIPLPRYGSAGSLSSANSSLTGNKEIPDSPEQYEVQKQQKEVWETGIEIFSRKPGKGVQYLQEQGLLGTSQEDVARWLHLD-ERLDKTAIGDFLGDHNHNQVMYHYIDQMNFAERDLVTALRYFLEGFRLPGEAQKIDRLMEKFASRYCECNPNNGLFTSADTAYILGFSIIMLTTDLHSPQVKNKMTKEQYIRLNRRNSDNEDLPEEYLSRIYDEIAGNEIKMKSNPNNSRLAGKQLISSEKKRRLLWNMEMEVISTAAKNLMESVSHVQAPFTTAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLTANSPITEMKAKNIDTIKTLITVAHTDGNYLGSSWLDVVKCISQLELAQLIGTGVRPQLLGPPNNLNLSSLDPSVKESIGETSSQSVVVAVDRIFTGSTRLDGDAIVEFVKALCQVSLEELAHPTQPRMFSLTKIVEISYYNMGRIRLQWSRIWQVLGDHFDRVGCSPRQDIAFFAVDSLRQLATKFIEKGEFANFRFQKDFLRPFEHIMKKNRSPVIRDMVVRCVAQIVHSQAPNIRSGWKNIFSVFHHAASDRDESVVELAFSMTGKIINELYAEDFSIMVDSFQDAVKCLSEFACNAFFPDTSMEAIRLIRSCASYIDANPNLFAEGMMDDSGMVSEEDRAWVRGWFPLLFELSCVVSRCKLDVRTRALTVLFDVVKTHGSSFKPHWWKDLFQVLFRIFDNMKLPEQHTEKAEWMTTTCNHALYAIVDVFSQFYDILGPLLLEQLYFQLLWCVQQDNEQLARSGTNCLENLVISNGIKFDEQTWQKTCQCVLDIFESTLPSALLTWKPHPPNKDNSAQSLNIEVLPKAKLFSALQIKCVVQLELIQTIDNIVFYPATSRKEDQENLALAQADMLNGKSASADQQKEEQGMYCALTTNHLLQLVECLLRSHRFAKSFNSDHEQRNVLWKA--SFRGNMKPNLLKQETQSLACALRILFKMYSDEAHRTDWSKIEARLVEVACEALEYFLALANEAHRDAWTPILLLLLTRIIKMSDHRFAIHASTCYPCLCEVMCFDLKPELRSILRRFFLRIGPVFRI 1642          
BLAST of GF14256 vs. nr
Match: gi|985412762|ref|XP_015371907.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Diuraphis noxia])

HSP 1 Score: 1254.97 bits (3246), Expect = 0.000e+0
Identity = 721/1635 (44.10%), Postives = 989/1635 (60.49%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEPG-QLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTME-SASAVSLPSHEPETA-----SEAVH--------VVRPILLDLA-----------DHLVAREFLAPEVYRNM----------------------MQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP--------------HQAEKDAVPE-------------------TPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKG--INDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLE---SHARPST---------LVMDVTS-----EANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFR-------------------IFEVGK-----FDMN--------------DLSLRDHR----EWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDTSVIVYKEED----------IVFGRVHVHLDVLEVSMNVL-----------------TSTAYGQTIE-------GPPWFEVLMPDH-FQVLKKLLRSLEQSFNESKSVLEDTPNSSR-KRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L +KD+K+  + ELR+ CE AL  +++   +       S LP  + G   I  E+   PFELAC QSK+ ++V+ SLD LQKL+AYGHL  + T     ++ G P L D++V ++   F+G QTDE + LQ++KALLT++TS +V VH+ ++L +V+TCYNIYLASRN++NQTTA+ATL+Q+LN +F+ ME  A  V +P +E E        E V         +   I+ DL            D  +  + LA ++ R+M                      +Q+DA+LVFRSLCKLSMKPLPEG PDPKSHELRSKILSL LLLSILQN+G  F+ N +F++AIK +LCVALS NG S+V EVFELSLA+F+SLL++++ HLK QIE+FFKEI LNILE SSS+FE KWMV+Q +  IC DAQ V+D++VNYDCDL AAN+F+RLVDDL+K+AQG+QA E+GAT NQ   MRIR LECL  ILKCMVEWSK+LYVNP              + A +  V +                     EA D  P  L+ +K +K V E GI +F  KP +GI +L E  ++     EVA++ H +  RLDKTAIG+FLGE E  NKE MY+YVD++NF   D V+ALR FL  FRLPGEAQKIDRL+EKFASR+ ECNP   LF SAD  YVL+YS+IMLTTDLHS  VK KMTKE++++ N+G  I+D++D+P EYLSKIYD IA +EIKMK T +   K +I + K+R +LWN E E+I+  A  LMES S     FT AK L+HVKPMFK+ W+P LA FSVGLQ  DD+ +  LCL+GIRC IR+A IF+ +LERDA++QALARFTLLT NS V EM++KNIE IKTLI+VA+TDGNYL  SWLDI+KCISQLE AQ++G+ G  P  L    S   P             +D +      E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS  E+ S  + RM+SL K+VEISYYNM RIRL+WSR+W V+G HFN VGC  +++++ FA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF  F LAA D   +IV +S +TTQ ++      N   + ESFQD + C+     N    +I   ++ +L    D V    Q F E              W R  + +LF                    +FE+ K     F +N              ++  ++H     EW+  TCNHAL ++ DVF++++++L  ++L DL +Q QWCIQQ N+QL++S+  C + L+ +N +K +   +      +  +         +E++          ++  +  + L+++    N++                  +T   Q  E           F  L  +H F +   LLR    +   + ++L++ P  S    D+S ++ QET++  C+L I+++              +    + + +   Y+LS+     +  W+ +L  I  ++    E  F    S+ Y L+C+++SI +  E + VL ++  RIG ++ I
Sbjct:   10 MFIIRALEKILGDKDIKKSYHAELRKECEAALQSIKSECQAEEKTDTSSALPLPKDGTSAISAEKYFRPFELAC-QSKSPRIVVTSLDCLQKLIAYGHLTGNCT-----NDTGKP-LIDKIVETICDCFTGCQTDEGILLQIIKALLTVVTSQHVEVHEATILKAVKTCYNIYLASRNLVNQTTARATLTQMLNVIFTRMEMQAMEVDVPKYESEIVEMCEVDETVQSDDLVNQDIDESIIYDLVNDICDSAIQSIDQGIKTKELAQDIIRSMSQESLETTNEAECKTITSFNHILQKDAFLVFRSLCKLSMKPLPEGTPDPKSHELRSKILSLHLLLSILQNAGPVFRTNPMFITAIKQFLCVALSNNGTSSVPEVFELSLAIFLSLLSRFKTHLKMQIEVFFKEIFLNILETSSSTFEHKWMVIQALTRICADAQSVVDMYVNYDCDLSAANLFQRLVDDLSKIAQGRQAIELGATLNQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNSQTNLGPERNVREYDANESGVTQDSLNSFDGSESSLTSNSSGNKEAPDM-PQQLQVLKHQKEVWETGILMFNRKPKQGIHFLQEQKLLGTSXSEVAEWFHVD-ERLDKTAIGDFLGENEDFNKEVMYYYVDQMNFVGKDVVTALRFFLDGFRLPGEAQKIDRLLEKFASRWFECNPNNGLFDSADTLYVLSYSIIMLTTDLHSPQVKTKMTKEQYIKMNRGSCISDNKDLPEEYLSKIYDDIAGHEIKMKST-VKPGKQLIPNEKRRKVLWNMEMEAIAVAAKNLMESVSHVRAPFTEAKHLEHVKPMFKMTWTPFLAAFSVGLQDCDDLEIAMLCLDGIRCAIRIACIFHMTLERDAYVQALARFTLLTANSPVTEMKAKNIETIKTLITVAYTDGNYLGSSWLDIIKCISQLELAQMVGT-GVRPQFLSQVISKTYPPIEALSQKLNFTTLDSSGKGSIGETSSQSVVVAVDRIFTGSTRLDGDAIVEFVKALCQVSINELASSTNPRMFSLQKIVEISYYNMGRIRLQWSRMWQVLGEHFNRVGCYPNEDIATFALDSLRQLSMKFIEKGEFANFKFQKDFLRPFEIIMKKNHSPTIRDMVVRCITQMVNSQAANIRSGWKNIFGVFHLAASDHDGAIVDMSFRTTQHVINLFYKQNCIIMMESFQDAVKCLSEFACNVGFPEISMEAIHLLRTCADCVHDKPQIFAEHFSEEPSSIEEDGVWVRGWFPLLFELSCIVSRCKLDIRTRALTVLFELVKTHGTVFKLNWWKDLFNVLFRIFDNVKHQEHSVERIEWMTITCNHALTSIVDVFTQYFDILCAILLQDLYAQLQWCIQQDNDQLAQSSTYCLENLITSNGSKFDKVNWDCTCQTIVSMFDSMMPKPLQEQNLKSEHGKPDILITAKCAIRLELIRTVDNIVFFPSTSRKEDQEYLALAQNTTINQNTEIVEQQQEEQGMFSNLSSEHLFMLTDCLLRVHNYTKQYNYTLLQNIPQRSYVNDDISNMLTQETQSVACVLRIVLKAICDESRRMHWTHAQNVASTVVNDSFQYYLSLNNHYHRTSWNPILLLIFTKLYKLPEDKFAVQSSTHYPLLCEMISIDLKPELKTVLARIFSRIGPVFKI 1633          
BLAST of GF14256 vs. nr
Match: gi|1028720451|ref|XP_016663116.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Acyrthosiphon pisum])

HSP 1 Score: 1249.96 bits (3233), Expect = 0.000e+0
Identity = 717/1635 (43.85%), Postives = 988/1635 (60.43%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEPGQ-LIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTME-SASAVSLPSHEPETA-----SEAVHVVRPILLDLADHLV-------------------AREFLAPEVYRNM----------------------MQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP--------------HQAEKDAVPE-------------------TPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKG--INDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLE---SHARPST---------LVMDVTS-----EANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFR-------------------IFEVGK-----FDMN--------------DLSLRDHR----EWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDTSVIVYKEED----------IVFGRVHVHLDVLEVSMNVL-----------------TSTAYGQTIE-------GPPWFEVLMPDH-FQVLKKLLRSLEQSFNESKSVLEDTPNSSR-KRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L +KDVK+  + ELR+ CE AL  +++   +       S LP  + G   I  E+   PFELAC QSK+ ++V+ SLD LQKL+AYGHL  + T     ++ G P L D++V ++   F+G QTDE + LQ++KALLT++TS +V VH+ ++L +V+TCYNIYLASRN++NQTTA+ATL+Q+LN +F+ ME  A  V +P +E E        E V    P+  D+ + ++                     + LA ++ R+M                      +Q+DA+LVFRSLCKLSMKPLPEG PDPKSHELRSKILSL LLLSILQN+G  F+ N +F++AIK +LCVALS NG S+V EVFELSLA+F+SLL++++ HLK QIE+FFKEI LNILE SSS+FE KWMV+Q +  IC DAQ V+D++VNYDCDL AAN+F+RLVDDL+K+AQG+QA E+GAT NQ   MRIR LECL  ILKCMVEWSK+LYVNP              + A + AV +                     EA D  P  L+ +K +K V E GI +F  KP  GI +L E  ++     ++A++ H +  RLDKTAIG+FLGE E  NKE MY+YVD++NF   D V+ALR FL  FRLPGEAQKIDRL+EKFASR+ ECNP   LF SAD  YVL+YS+IMLTTDLHS  VK KMTKE++++ N+G  I+D++D+P EYLSKIYD IA +EIKMK T +   K +I + K+R +LWN E E+I+  A  LMES S     FT AK L+HVKPMFK+ W+P LA FSVGLQ  DD+ +  LCL+GIRC IR+A IF+ +LERDA++QALARFTLLT NS V EM++KNIE IKTLI+VA+TDGNYL  SWLDI+KCISQLE AQ++G+ G  P  L    S   P             +D +      E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC +S  E+ +  + RM+SL K+VEISYYNM RIRL+WSR+W V+G HFN VGC  +++++ FA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF  F LAA D   +IV +S +TTQ ++      N   + ESFQD + C+     N    +I   ++ +L    D V    Q F E              W R  + +LF                    +FE+ K     F +N              ++  ++H     EW+  TCNHAL ++ DVF++++++L  V+L DL +Q QWCIQQ N+QL++S+  C + L+ +N +K +   +      +  +         +E++          ++  +  + L+++    N++                  +T   Q  E           +  L  +H F +   LLR    +   + ++L++ P  S    D+S ++ QET++  C+L I ++              +    + + +   Y+LS+     +  W+ +L  I  ++    E  F    S+ Y L+C+++SI +  E + VL ++  RIG ++ I
Sbjct:   10 MFIIRALEKILGDKDVKKSYHAELRKECEAALQSIKSECQAEEKTDASSALPLPKDGSSAISAEKYFRPFELAC-QSKSPRIVVTSLDCLQKLIAYGHLTGNCT-----NDTGKP-LIDKIVETICDCFTGCQTDEGILLQIIKALLTVVTSQHVEVHEATILKAVKTCYNIYLASRNLVNQTTARATLTQMLNVIFTRMEMQAMEVDVPKYESEIVELCEVDETVQSDDPLNQDIDESIIYDIVNTICDSAIESIDQGTKTKELAQDIVRSMSQESLETTNEAECKTIASFNHILQKDAFLVFRSLCKLSMKPLPEGTPDPKSHELRSKILSLHLLLSILQNAGPVFRTNPMFITAIKQFLCVALSNNGTSSVPEVFELSLAIFLSLLSRFKTHLKMQIEVFFKEIFLNILETSSSTFEHKWMVIQALTRICADAQSVVDMYVNYDCDLSAANLFQRLVDDLSKIAQGRQAIELGATLNQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNSQTNLGPERNVREYDANESAVTQDSLNSFDGSESSLTSNSSGNKEAPDM-PQQLQVLKHQKEVWETGILMFNRKPKHGINFLQEQKLLGTSPSDIAEWFHVD-ERLDKTAIGDFLGENEDFNKEVMYYYVDQMNFVGKDVVTALRFFLDGFRLPGEAQKIDRLLEKFASRWFECNPNNGLFDSADTLYVLSYSIIMLTTDLHSPQVKTKMTKEQYIKMNRGSCISDNKDLPEEYLSKIYDDIAGHEIKMKST-VKPGKQLIPNEKRRKVLWNMEMEAIAVAAKNLMESVSHVRAPFTEAKHLEHVKPMFKMTWTPFLAAFSVGLQDCDDLEIAMLCLDGIRCAIRIACIFHMTLERDAYVQALARFTLLTANSPVTEMKAKNIETIKTLITVAYTDGNYLGTSWLDIIKCISQLELAQMVGT-GVRPQFLSQVISKTYPPIEALSQKLNFTTLDSSGKGSIGETSSQSVVVAVDRIFTGSTRLDGDAIVEFVKALCQISINELANSTNPRMFSLQKIVEISYYNMGRIRLQWSRMWQVLGEHFNRVGCYPNEDIATFALDSLRQLSMKFIEKGEFANFKFQKDFLRPFEIIMKKNHSPTIRDMVVRCITQMVNSQAANIRSGWKNIFGVFHLAASDHDGAIVDMSFRTTQHVINLFYKQNCIIMMESFQDAVKCLSEFACNVGFPEISMEAIHLLRTCADCVHDKPQIFAEHFSEEPSSLEEDGVWVRGWFPLLFELSCIVSRCKLDIRTRALTVLFELVKTHGTVFKLNWWKDLFNVLFRIFDNVKHQEHSVERIEWMTITCNHALTSIVDVFTQYFDILCAVLLQDLYAQLQWCIQQDNDQLAQSSTYCLENLITSNGSKFDKVNWDCTCQTIVSMFDSMMPKPLQEQNLRSEPVKPDILITAKCAIRLELIRTVDNIVFFPSTSRKEDQEYLALAQNTTINQNTEILEQQQEEQGMYSNLSSEHLFMLTDCLLRVHNYTKQYNYTLLQNIPQRSYVNDDISNMLTQETQSVACVLRIALKAICDESRRVHWTHAQNVASTVVNDSFQYYLSLNNHYHRTSWNPILLLIFTKLYKLPEDKFAVQSSTHYPLLCEMISIDLKPELKTVLGRIFSRIGPVFKI 1633          
BLAST of GF14256 vs. nr
Match: gi|1230208436|ref|XP_022179292.1| (brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Myzus persicae])

HSP 1 Score: 1249.19 bits (3231), Expect = 0.000e+0
Identity = 720/1635 (44.04%), Postives = 992/1635 (60.67%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRALPASNTSDGQCSVLPEIEPGQ-LIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTME-SASAVSLPSHEPETA-----SEAVH------------VVRPILLDLADHLV-------AREFLAPEVYRNM----------------------MQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNP--------------HQAEKDAVPE-------------------TPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKG--INDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLE---SHARPST---------LVMDVTS-----EANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTIL---FDIVKQHGQSFEES-------------WWRDLYQVLFR-------------------IFEVGK-----FDMN--------------DLSLRDHR----EWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEA----DTFGLIISFLADII------KDTSVIVYKEEDIVFGRVHVHLDVLEVSMNVL-----------------TSTAYGQTIE-------GPPWFEVLMPDH-FQVLKKLLRSLEQSFNESKSVLEDTPNSSR-KRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L +KDVK+  + ELR+ CE AL  +++   +       S LP  + G   I  E+   PFELAC QSK+ ++V+ SLD LQKL+AYGHL  + T     ++ G P L D++V ++   F+G QTDE + LQ++KALLT++TS +V VH+ ++L +V+TCYNIYLASRN++NQTTA+ATL+Q+LN +F+ ME  A  V +P +E E        E V             ++  I+ D+ D  +         + LA ++ R+M                      +Q+DA+LVFRSLCKLSMKPLPEG PDPKSHELRSKILSL LLLSILQN+G  F+ N +F++AIK +LCVALS NG S+V EVFELSLA+F+SLL++++ HLK QIE+FFKEI LNILE SSS+FE KWMV+Q +  IC DAQ V+D++VNYDCDL AAN+F+RLVDDL+K+AQG+QA E+GAT NQ   MRIR LECL  ILKCMVEWSK+LYVNP              + A + A+ +                     EA D  P  L+ +K +K V E GI +F  KP  GI +L E  ++     ++A++ H +  RLDKTAIG+FLGE E  NKE MY+YVD++NF   D V+ALR FL  FRLPGEAQKIDRL+EKFASR+ ECNP   LF SAD  YVL+YS+IMLTTDLHS  VK KMTKE++++ N+G  I+D++D+P EYLSKIYD IA +EIKMK T +   K +I + K+R +LWN E E+I+  A  LMES S     FT AK L+HVKPMFK+ W+P LA FSVGLQ  DD+ +  LCL+GIRC IR+A IF+ +LERDA++QALARFTLLT NS V EM++KNIE IKTLI+VA+TDGNYL  SWLDI+KCISQLE AQ++G+ G  P  L    S   P             +D +      E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS  E+ +  + RM+SL K+VEISYYNM RIRL+WSR+W V+G HFN VGC  +++++ FA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF  F LAA D   +IV +S +TTQ ++      N   + ESFQD + C+     N    +I   ++ +L    D V    Q F E              W R  + +LF                    +FE+ K     F +N              ++  ++H     EW+  TCNHAL ++ DVF++++++L  ++L DL +Q QWCIQQ N+QL++S+  C + L+ +N +K +      T   I+S    ++      ++      K + ++  +  + L+++    N++                  +T   Q  E           +  L  +H F +   LLR    +   + ++L++ P  S    D+S ++ QET++  C+L I ++              +    + + +   Y+LS+     +  W+ +L  I  ++    E  F    S+ Y L+C+++SI +  E + VL ++  RIG ++ I
Sbjct:   10 MFIIRALEKILGDKDVKKSYHAELRKECEAALQSIKSECQAEEKTDASSALPLPKDGSSAISAEKYFRPFELAC-QSKSPRIVVTSLDCLQKLIAYGHLTGNCT-----NDTGKP-LIDKIVETICDCFTGCQTDEGILLQIIKALLTVVTSQHVEVHEATILKAVKTCYNIYLASRNLVNQTTARATLTQMLNVIFTRMEMQAMEVDVPKYESEIVELCEVDETVQSDDSLNIDIDESIIYDIVNDICDAAIQSIDQGNKTKELAQDIVRSMSQESLETTNEAECKTVASFNHILQKDAFLVFRSLCKLSMKPLPEGTPDPKSHELRSKILSLHLLLSILQNAGPVFRTNPMFITAIKQFLCVALSNNGTSSVPEVFELSLAIFLSLLSRFKTHLKMQIEVFFKEIFLNILETSSSTFEHKWMVIQALTRICADAQSVVDMYVNYDCDLSAANLFQRLVDDLSKIAQGRQAIELGATLNQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNSQTNLGPERNVREYDANESAITQDSLNSLDGSESSLTSNSSGNKEAPDM-PQQLQVLKHQKEVWETGILMFNRKPKHGINFLQEQKLLGTSPSDIAEWFHVD-ERLDKTAIGDFLGENEDFNKEVMYYYVDQMNFVGKDVVTALRFFLDGFRLPGEAQKIDRLLEKFASRWFECNPNNGLFDSADTLYVLSYSIIMLTTDLHSPQVKTKMTKEQYIKMNRGSCISDNKDLPEEYLSKIYDDIAGHEIKMKST-VKPGKQLIPNEKRRKVLWNMEMEAIAVAAKNLMESVSHVRAPFTEAKHLEHVKPMFKMTWTPFLAAFSVGLQDCDDLEIAMLCLDGIRCAIRIACIFHMTLERDAYVQALARFTLLTANSPVTEMKAKNIETIKTLITVAYTDGNYLGTSWLDIIKCISQLELAQMVGT-GVRPQFLSQVISKTYPPIEALSQKLNFTTLDSSGKGSIGETSSQSVVVAVDRIFTGSTRLDGDAIVEFVKALCQVSINELANSTNPRMFSLQKIVEISYYNMGRIRLQWSRMWQVLGEHFNRVGCYPNEDIATFALDSLRQLSMKFIEKGEFANFKFQKDFLRPFEIIMKKNHSPTIRDMVVRCITQMVNSQAANIRSGWKNIFGVFHLAASDHDGAIVDMSFRTTQHVINLFYKQNCIIMMESFQDAVKCLSEFACNVGFPEISMEAIHLLRTCADCVHDKPQIFAEHFSEEPSSLEEDGVWVRGWFPLLFELSCIVSRCKLDIRTRALTVLFELVKTHGTVFKLNWWKDLFNVLFRIFDNVKHQEHSVERIEWMTITCNHALTSIVDVFTQYFDILCAILLQDLYAQLQWCIQQDNDQLAQSSTYCLENLITSNGSKFDKVNWDCTCQTIVSMFDSMMPKPLQEQNLRSEHVKPDTLITAKCAIRLELIRTVDNIVFFPSTSRKEDQEYLALAQNTTINQNTEILEQQQEEQGMYSNLSSEHLFMLTDCLLRVHNYTKQYNYTLLQNIPQRSYVNDDISNMLTQETQSVACVLRIALKAICDESRRVHWTHAQNVASTVVNDSFQYYLSLNNHYHKTSWNPILLLIFTKLYKLPEDKFAVQSSTHYPLLCEMISIDLKPELKTVLGRIFSRIGPVFKI 1633          
BLAST of GF14256 vs. nr
Match: gi|954560261|ref|XP_014603898.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 2 [Polistes canadensis])

HSP 1 Score: 1248.8 bits (3230), Expect = 0.000e+0
Identity = 729/1666 (43.76%), Postives = 992/1666 (59.54%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA----LPASNTSDGQCSVLPEIEP-GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPE-----------TASEAV-------------HVVRPILLDLADHLVAREFLA------------------------------PEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAEKDAVP--------------------------------ETPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLESHARP-----------------------STL---VMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFD---------------IVKQHGQSFEE--SWWRDLYQVLFR-------------------IFEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKD---TSVIVYKEE------------DIVFGRVHVHLDVLEVSMNVL-------------TSTAYGQTIEGPP-----------------WFEVLMPDH-FQVLKKLLRS--LEQSFN---ESKSVLEDTPNSSRKRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L +KDVKR    +L+ +CE AL DLR     +P +   +   + LP+ +    +I  E+   PFELAC QSK+ ++V+ +LD LQKL+AYGHL      +P +  P    L  R+V ++   F GPQTDE VQLQ++KALLT++TS +V VH+ ++LL++RT YN+YLASRN++NQTTA+ATL+Q++N +F+ ME+ +       EPE           T +E V             HV+   +LD   H V  E  A                                 + +++Q+DA+LVFR+LCKLSMKPLP+G PDPKSHELRSKILSL+LLL ILQN+G   + N++FV AIK YLCVALS+NGVS+V EVFELSLALF++LL +++VHLK QIE+FFKEI +NILE SSSSFE KWMV+  +  IC DAQ V+DI+VNYDCDL AAN+FERLV+DL+K+AQG+QA E+GA+PNQ   MRIR LECL  ILKCMVEWS++LYVNP       +P                                E P++    P   E  KQ+K V E GI +F  KP KG++YL E  ++    ++VA++LH +  RLDKTAIG+FLG+  + + MY+Y+D++NFA+ D V+ALR FL  FRLPGEAQKIDRLMEKFASRY ECNP   LF SAD AY+L +S+IMLTTDLHS  VK KMTKE++++ N+ I+D+ED+P EYLSKIYD IA NEIKMK       K +I+  K+R LLWN E E IS  A  LMES S     FT+AK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ +LERDA++QALARFTLLT NS + EM++KNI+ IKTLI+VAHTDGNYL  SWLD++KCISQLE AQL+G+ G  P  L   ++P                       S+L   V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS EE+      RM+SL K+VEISYYNM RIRL+WSRIW V+G+HF+ VGC   Q ++FFAVDSL+QL+ KF+EKGE  NF FQKDFLRPFE+IMK N+SP IRDMVVRC++Q++ SQ+ NI+SGWKNIF+ F  AA D  ES+V L+   T +I+ +L +++   + +SFQD + C+     N S  D    ++ ++                 ++   G   EE  +W R  + +LF                    +F+V K                     FD   L  +  +  EW+ TTCNHALYA+ DVFS+FY+ L  ++L  L SQ  WC+QQ NEQL++S  +C + LV +N  K +  T+      + DI +    T++I +K                +  +  V L++++   NV+              + A    + G P                  +  L   H  Q+++ LL+S    ++FN   E + +L  +      +  + L+ QE ++  C L IL ++ +          +E   V      L YFL+++ +   + W  +L  +  +IL   +  F    SS Y L+C+++   +  E R VL +   RIG ++ I
Sbjct:   11 MFIVRALEKILADKDVKRSHLSQLKRSCESALEDLRNEIKDVPVAQGEETTSNALPQPKSDSNVISAEKYFLPFELAC-QSKSPRIVVTALDCLQKLIAYGHLT---GNVPDSTEPNK-LLIVRIVETICSCFMGPQTDEGVQLQIIKALLTVMTSQHVEVHEGTVLLTIRTVYNVYLASRNLVNQTTARATLTQMINVIFARMETQAEEENVRTEPEQQQDTSASSGDTETEIVNNDEVPTENVCESHVIVKSILDDIIHSVVPEDAANVATSEEASLDQVPAEDNTDEAVAESDNMVTAKFTHVLQKDAFLVFRALCKLSMKPLPDGTPDPKSHELRSKILSLQLLLGILQNAGPVLRSNEMFVIAIKQYLCVALSKNGVSSVPEVFELSLALFLALLARFKVHLKMQIEVFFKEIFMNILETSSSSFEHKWMVIHALTRICADAQSVVDIYVNYDCDLSAANLFERLVNDLSKIAQGRQALELGASPNQEKSMRIRGLECLVSILKCMVEWSRDLYVNPSVPADQQLPSEPPDLPLEPPLPRYGSAGSLSSANSSLVGNKEIPDS----PEQYEVQKQQKEVWEAGIDIFNRKPSKGVQYLQEQSLLGTSPEDVARWLHMD-ERLDKTAIGDFLGDHNHNQVMYNYIDQMNFADRDLVTALRYFLEGFRLPGEAQKIDRLMEKFASRYCECNPNNGLFTSADTAYILGFSIIMLTTDLHSPQVKNKMTKEQYIKLNRRISDNEDLPEEYLSKIYDEIAGNEIKMKSNPNRPGKQVISSEKKRRLLWNMEMEVISTAAKNLMESVSHVQAPFTTAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLDGIRCAIRIACIFHMTLERDAYVQALARFTLLTANSPITEMKAKNIDTIKTLITVAHTDGNYLGSSWLDVVKCISQLELAQLIGT-GVRPQLLGPPSKPHFPSPLFTNLAHNNSYQNNGLNLSSLDPSVKESIGETSSQSVVVAVDRIFTGSTRLDGDAIVAFVKALCQVSLEELSHPTQPRMFSLTKIVEISYYNMGRIRLQWSRIWQVLGDHFDRVGCSPRQEIAFFAVDSLRQLATKFIEKGEFANFRFQKDFLRPFEHIMKKNRSPVIRDMVVRCVAQIVHSQAPNIRSGWKNIFSVFHHAASDRDESVVELAFSMTGKIINELYAEDFSIMVDSFQDAVKCLSEFACNASFPDTSMEAIRLMRSCASYIVANPNLFAEGMMDDSGIVSEEDRAWVRGWFPLLFELSCVVSRCKLDVRTRALTVLFDVVKTHGASFKPHWWKDLFQVLFRIFDNMKLPEQHTEKAEWMTTTCNHALYAIVDVFSQFYDTLGPLLLGQLYSQLLWCVQQDNEQLARSGTNCLENLVISNGIKFDEKTWDNTCRCVLDIFESTLPTALITWKPPSPNNENAKYKLFSALLIKCVVQLELIQTIDNVVFYPATSRKEDQENLALAQADMLNGKPSELGVQGGGDQQKEEQGMYCSLTTTHLLQLVECLLKSHRFAKTFNSNHEQRQLLWKSGFYCNMKSETGLLNQEIQSLACALRILFKMYSDEAHRNDWAKVETLLVEVACEALEYFLALSNEMHIDAWTPILLLLLTRILKMSDSRFAVHASSCYPLLCEVMCFDLKPELRSVLRRFFLRIGPVFRI 1665          
BLAST of GF14256 vs. nr
Match: gi|1189084159|ref|XP_020810363.1| (brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Drosophila serrata])

HSP 1 Score: 1241.87 bits (3212), Expect = 0.000e+0
Identity = 678/1398 (48.50%), Postives = 900/1398 (64.38%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA--LPASNTSDGQ---CSVLP--EIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPETASEAVHVVRPI------LLDLADHLVAREFLA---------------------PEV---------------------------YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAEKDAVPETPEATDED---------------------------PAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELEN--KEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSE-DIPPEYLSKIYDAIASNEIKMKGTSLA-------NNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSS------GHGPDGLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCVINKSKLDIRTRSLTILFDIVKQHGQSFEES----------------------WWRDLYQVLFR-------------------IFEVGK--------------FDM-----NDLSLRDH----REWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            MF+ +A+E +L +KD++R  + +L+++C+ AL  ++A  + A   ++G    C+ LP  + +   +I+ E    PFELAC+ S++ ++V+ +LD LQKL+AYGHL  S   +  + NPGH  L DR+V ++   FSGPQTDE VQLQ++KALLT++TS +V +H+ +LL +VRTCY+IYL+S+N++NQTTA+ATL+Q+LN +F+ ME+     LP       + ++H            L  AD ++A E LA                     P V                           + +++Q+DA+LVFRSLCKLSMKPLPEG PDPKSHELRSK+LSL LLL ILQN+G  F+ N++F+ AIK YLCVALS NGVS V EVFELSL++F++LL+ ++VHLK QIE+FFKEI LNILEA+SSSFE KWMV+Q +  IC DAQ V+DI+VNYDCD  AAN+FERLV+DL+K+AQG+QA E+GA P Q   MRIR LECL  ILKCMVEWSK+LYVNP+        ++P AT++D                           P  LE  K RK V+E GI+LF  KP KG+++L E  ++    +++A +LH++  RLDKT IG +LGE ++  K  M  Y+D+ NF +L+ V+ALR+ L  FRLPGEAQKIDRLMEKFASRY ECNP  +LF+SAD  YVLA+S+IMLTTDLHS  VK KMTKE++++ N+GI+DS+ D+P EYLS IYD IA +EIKMK  S           +  IT+ K+R LLWN E E IS TA  LM+S S     FTSAK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ SLERDA++QALARFTLL  NS +NEM++KNI+ IKTLI VAHTDGNYL  SWLDI+KCISQLE AQL+G+             L+    PS  V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS +E+   Q  RM+SL K+VEISYYNMERIRL+WSRIW V+G HFN VGC  ++ +SFFA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE+IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAAGD  E IV L+ QTT +I+  L       + +SFQD + C+   +       S+  +  +V+   Q   E+                      W R  + +LF                    +FE+ K              F++     +++ L +H     EW+ TTCNHALYA+ DVF+++++VL  ++L +L +Q  WC+QQ NEQL++S  +C + LV +N  K    T+      + DI   T
Sbjct:   11 MFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLPKNDAASIINAETYFLPFELACK-SRSPRIVVTALDCLQKLIAYGHLTGS---IQDSANPGH-LLIDRIVVTIYGCFSGPQTDEGVQLQIIKALLTVVTSQHVEIHETTLLQAVRTCYDIYLSSKNLVNQTTARATLTQMLNVIFARMEN-QVYELPPPPSHQMNGSIHSEDWTNGGGEEALGDADEVIASEILAEIITAAYNEATKDQESGGDAEPIVNGNDSSSHSDHDSVELHSENDAVVTAKFTHILQKDAFLVFRSLCKLSMKPLPEGHPDPKSHELRSKVLSLHLLLLILQNAGPVFRSNEMFIMAIKQYLCVALSNNGVSLVPEVFELSLSIFVALLSNFKVHLKRQIEVFFKEIFLNILEANSSSFEHKWMVIQALTRICADAQSVVDIYVNYDCDFSAANLFERLVNDLSKIAQGRQALELGANPMQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNMPAPALQVQSPTATEQDQADITIQTAHSGSSHSLNSNQEQLQDLPEALEERKMRKEVMETGIELFNRKPKKGVQFLQEKQLLGPSCQDIAHWLHED-ERLDKTVIGNYLGENDDHSKAVMCAYIDDFNFRQLEIVAALRILLEEFRLPGEAQKIDRLMEKFASRYCECNPQNQLFQSADTVYVLAFSIIMLTTDLHSPQVKHKMTKEQYIKMNRGISDSKGDLPEEYLSSIYDEIAEHEIKMKNNSGMLQQPKPLGKQAFITE-KRRKLLWNMEMEVISLTATNLMQSVSHVKSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCISQLELAQLIGTGVRPQYLSGAQTTLKDSLNPS--VKEHIGETSSQSVVVAVDRIFTGSMRLDGDAIVDFVKALCQVSVDELQQPQP-RMFSLQKIVEISYYNMERIRLQWSRIWQVLGEHFNAVGCNSNEEISFFALDSLRQLSMKFMEKGEFSNFRFQKDFLRPFEHIMKKNASPAIRDMVVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDHEEPIVELAFQTTGKIIGDLYQRQFAIMVDSFQDAVKCLSEFATARFPDTSMEAI-RLVRTCAQCVHEAPQLFAEHAGMENDASVAEEDRVWVRGWFPMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPNWWKDLFNVIFRIFDNMKLPEHVTEKSEWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCVQQSNEQLARSGTNCLENLVISNGFKFNESTWDKTCQCILDIFNAT 1396          
BLAST of GF14256 vs. nr
Match: gi|1036705398|ref|XP_017037331.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Drosophila kikkawai])

HSP 1 Score: 1240.71 bits (3209), Expect = 0.000e+0
Identity = 677/1397 (48.46%), Postives = 898/1397 (64.28%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA--LPASNTSDGQ---CSVLP--EIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMES-ASAVSLPSHEPETAS----EAVHVVRPILLDLADHLVAREFLAPEV------------------------------------------------YRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAEKDAVPETPEATDED---------------------------PAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELEN--KEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSE-DIPPEYLSKIYDAIASNEIKMKGTSLA-------NNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSS------GHGPDGLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCVINKSKLDIRTRSLTILFDIVKQHGQSFEES----------------------WWRDLYQVLFR-------------------IFEVGK--------------FDM-----NDLSLRDH----REWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            MF+ +A+E +L +KD++R  + +L+++C+ AL  ++A  + A   ++G    C+ LP  + +   +I+ E    PFELAC+ S++ ++V+ +LD LQKL+AYGHL  S   +  + NPGH  L DR+V ++   FSGPQTDE VQLQ++KALLT++TS +V +H+ +LL +VRTCY+IYL+S+N++NQTTA+ATL+Q+LN +F+ ME+    V  P   P   S    +  +      L  AD ++A E LA  +                                                + +++Q+DA+LVFRSLCKLSMKPLPEG PDPKSHELRSK+LSL LLL ILQN+G  F+ N++F+ AIK YLCVALS NGVS V EVFELSL++F++LL+ ++VHLK QIE+FFKEI LNILEA+SSSFE KWMV+Q +  IC DAQ V+DI+VNYDCD  AAN+FERLV+DL+K+AQG+QA E+GA P Q   MRIR LECL  ILKCMVEWSK+LYVNP+        ++P AT++D                           P  LE  K RK V+E GI+LF  KP KG+++L E  ++    +++A +LH++  RLDKT IG +LGE ++  K  M  Y+D+ NF +L+ V+ALR+ L  FRLPGEAQKIDRLMEKFASRY ECNP  +LF+SAD  YVLA+S+IMLTTDLHS  VK KMTKE++++ N+GI+DS+ D+P EYLS IYD IA +EIKMK  S           +  IT+ K+R LLWN E E IS TA  LM+S S     FTSAK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL GIRC IR+A IF+ SLERDA++QALARFTLL  NS +NEM++KNI+ IKTLI VAHTDGNYL  SWLDI+KCISQLE AQL+G+             L+    PS  V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS +E+   Q  RM+SL K+VEISYYNMERIRL+WSRIW V+G HFN VGC  ++ +SFFA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE+IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAAGD  E IV L+ QTT +I+  L       + +SFQD + C+   +       S+  +  +V+   Q   E+                      W R  + +LF                    +FE+ K              F++     +++ L +H     EW+ TTCNHALYA+ DVF+++++VL  ++L +L +Q  WC+QQ NEQL++S  +C + LV +N  K    T+      + DI   T
Sbjct:   11 MFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLPKNDAASIINAETYFLPFELACK-SRSPRIVVTALDCLQKLIAYGHLTGS---IQDSANPGH-LLIDRIVVTIYGCFSGPQTDEGVQLQIIKALLTVVTSQHVEIHETTLLQAVRTCYDIYLSSKNLVNQTTARATLTQMLNVIFARMENQVYEVPPPPSHPMNGSVHSEDWTNGGGEEALGDADEVIASEILAEIITAAYNEATKDQESGGDAEPIANGNDSSSHSDHDSVELHSENDAVVTAKFTHILQKDAFLVFRSLCKLSMKPLPEGHPDPKSHELRSKVLSLHLLLLILQNAGPVFRSNEMFIMAIKQYLCVALSNNGVSLVPEVFELSLSIFVALLSNFKVHLKRQIEVFFKEIFLNILEANSSSFEHKWMVIQALTRICADAQSVVDIYVNYDCDFSAANLFERLVNDLSKIAQGRQALELGANPMQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNMPAPPLQVQSPTATEQDQADITIQTAHSGSSHSLNSNQEQLQDLPEALEERKMRKEVMETGIELFNRKPKKGVQFLQEKQLLGPTCQDIAHWLHED-ERLDKTVIGNYLGENDDHSKAVMCAYIDDFNFRQLEVVAALRILLEEFRLPGEAQKIDRLMEKFASRYCECNPQNQLFQSADTVYVLAFSIIMLTTDLHSPQVKHKMTKEQYIKMNRGISDSKGDLPEEYLSSIYDEIAEHEIKMKNNSGMLQQPKPLGKQAFITE-KRRKLLWNMEMEVISLTATNLMQSVSHVKSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLNGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCISQLELAQLIGTGVRPQYLSGAQTTLKDSLNPS--VKEHIGETSSQSVVVAVDRIFTGSMRLDGDAIVDFVKALCQVSVDELQQPQP-RMFSLQKIVEISYYNMERIRLQWSRIWQVLGEHFNAVGCNSNEEISFFALDSLRQLSMKFMEKGEFSNFRFQKDFLRPFEHIMKKNASPAIRDMVVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDHEEPIVELAFQTTGKIIGDLYQRQFAIMVDSFQDAVKCLSEFATARFPDTSMEAI-RLVRTCAQCVHEAPQLFAEHAGMENDASVAEEDRVWVRGWFPMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPNWWKDLFNVIFRIFDNMKLPEHVTEKSEWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCVQQSNEQLARSGTNCLENLVISNGFKFNESTWDKTCQCILDIFNAT 1396          
BLAST of GF14256 vs. nr
Match: gi|972195788|ref|XP_015179780.1| (PREDICTED: brefeldin A-inhibited guanine nucleotide-exchange protein 1 [Polistes dominula])

HSP 1 Score: 1240.71 bits (3209), Expect = 0.000e+0
Identity = 725/1663 (43.60%), Postives = 995/1663 (59.83%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA----LPASNTSDGQCSVLPEIEP-GQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEPE-----------TASEAVH--------------VVRPILLDLADHLVAREF-----------------------------LAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPH-----------------------------QAEKDAVPETPEATDEDPAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELENKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSEDIPPEYLSKIYDAIASNEIKMKGTSLANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSSGHGPDGLESHARP-----------------------STL---VMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQDCLSCV----INKSKLDIRTRSLTILFD---------------IVKQHGQSFEE--SWWRDLYQVLFR-------------------IFEVGK---------------------FDMNDLSLR--DHREWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKD---TSVIVYKEE------------DIVFGRVHVHLDVLEVSMNVL-------------TSTAYGQTIEGPP-----------------WFEVLMPDH-FQVLKKLLRS--LEQSFN---ESKSVLEDTPNSSRKRDLSILVKQETKAAECLLTILIELCTSNVGNEFQKSLEKYFVSFLVNVLGYFLSMTTKTQQEHWDRMLASISEQILNFQEPMFISAMSSLYNLICDILSIGVSKEFRGVLCQMLKRIGSLYDI 1435
            MF+ +A+E +L +KDVKR    +L+ +CE AL DLR     +P +   +   + LP+ +    +I  E+   PFELAC QSK+ ++V+ +LD LQKL+AYGHL      +P +  P    L  R+V ++   F GPQTDE VQLQ++KALLT++TS +V VH+ ++LL++RT YN+YLASRN++NQTTA+ATL+Q++N +F+ ME+ +       E E           T +E V+              +V+ IL D+ + +V  +                              +    + +++Q+DA+LVFR+LCKLSMKPLP+G PDPKSHELRSKILSL+LLL ILQN+G   + N++FV AIK YLCVALS+NGVS+V EVFELSLALF++LL +++VHLK QIE+FFKEI +NILE SSSSFE KWMV+  +  IC DAQ V+DI+VNYDCDL AAN+FERLV+DL+K+AQG+QA E+GA+PNQ   MRIR LECL  ILKCMVEWS++LYVNP                               +   ++    E  D  P   E  KQ+K V E GI +F  KP KG++YL E+ ++    ++VA++LH +  RLDKTAIG+FLG+  + + MY+Y+D++NFA+ D V+ALR FL  FRLPGEAQKIDRLMEKFASRY ECNP   LF SAD AY+L +S+IMLTTDLHS  VK KMTKE++++ N+ I+D+ED+P EYLSKIYD IA NEIKMK       K +I+  K+R LLWN E E IS  A  LMES S     FT+AK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ +LERDA++QALARFTLLT NS + EM++KNI+ IKTLI+VAHTDGNYL  SWLD++KCISQLE AQL+G+ G  P  L   ++P                       S+L   V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS EE+      RM+SL K+VEISYYNM RIRL+WSRIW V+G+HF+ VGC   Q ++FFAVDSL+QL+ KF+EKGE  NF FQKDFLRPFE+IMK N+SP IRDMVVRC++Q++ SQ+ NI+SGWKNIF+ F  AA D  ES+V L+   T +I+ +L +++   + +SFQD + C+     N S  D    ++ ++                 ++   G   EE  +W R  + +LF                    +F+V K                     FD   L  +  +  EW+ TTCNHALYA+ DVFS+FY+ L  ++L  L SQ  WC+QQ NEQL++S  +C + LV +N  K + +T+      + DI +    T++I +K                +  +  V L++++   NV+              + A    + G P                  +  L   H  Q++  LL+S    ++FN   E + + +     S K +  +L  QE ++  C L IL ++ +         ++E   V      L YFL+++ +   + W  +L  +  +IL   +  F    SS Y L+C+++   +  E R VL +   RIG ++ I
Sbjct:   11 MFIVRALEKILADKDVKRSHLSQLKRSCESALEDLRNEIKDVPVAQGEETTSNALPQPKSDSNVISAEKYFLPFELAC-QSKSPRIVVTALDCLQKLIAYGHLT---GNVPDSTEPNK-LLIVRIVETICSCFMGPQTDEGVQLQIIKALLTVMTSQHVEVHEGTVLLTIRTVYNVYLASRNLVNQTTARATLTQMINVIFARMETQAEEENVRTESEQQQDTSTSNGDTETEIVNNDEALTENVCESHVIVKSILDDVINSVVPEDAANVATSEEASLDQVPAEDNTDEAVAESDNMVTAKFTHVLQKDAFLVFRALCKLSMKPLPDGTPDPKSHELRSKILSLQLLLGILQNAGPVLRSNEMFVIAIKQYLCVALSKNGVSSVPEVFELSLALFLALLARFKVHLKMQIEVFFKEIFMNILETSSSSFEHKWMVIHALTRICADAQSVVDIYVNYDCDLSAANLFERLVNDLSKIAQGRQALELGASPNQEKSMRIRGLECLVSILKCMVEWSRDLYVNPSVPADQQLPSEPPDPPLEPPLPRYGSAGSLSSANSSLVGNKEIPDS-PEQYEVQKQQKEVWEAGIDIFNRKPSKGVQYLQEHGLLGTSPEDVARWLHID-ERLDKTAIGDFLGDHNHNQVMYNYIDQMNFADRDLVTALRYFLEGFRLPGEAQKIDRLMEKFASRYCECNPNNGLFTSADTAYILGFSIIMLTTDLHSPQVKNKMTKEQYIKLNRRISDNEDLPEEYLSKIYDEIAGNEIKMKSNPNRPGKQVISSEKKRRLLWNMEMEVISTAAKNLMESVSHVQAPFTTAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIASLCLDGIRCAIRIACIFHMTLERDAYVQALARFTLLTANSPITEMKAKNIDTIKTLITVAHTDGNYLGSSWLDVVKCISQLELAQLIGT-GVRPQLLGPPSKPHFPSPLFTNLAHNNSYQNNGLNLSSLDPSVKESIGETSSQSVVVAVDRIFTGSTRLDGDAIVAFVKALCQVSLEELSHPTQPRMFSLTKIVEISYYNMGRIRLQWSRIWQVLGDHFDRVGCSPRQEIAFFAVDSLRQLATKFIEKGEFANFRFQKDFLRPFEHIMKKNRSPIIRDMVVRCVAQIVHSQAPNIRSGWKNIFSVFHHAASDRDESVVELAFSMTGKIINELYAEDFSIMVDSFQDAVKCLSEFACNASFPDTSMEAIRLMRSCASYIVANPNLFAEGMMDDSGIVSEEDRAWVRGWFPLLFELSCVVSRCKLDVRTRALTVLFDVVKTHGASFKPHWWKDLFQVLFRIFDNMKLPEQHTEKAEWMTTTCNHALYAIVDVFSQFYDTLGPLLLGQLYSQLLWCVQQDNEQLARSGTNCLENLVISNGIKFDEETWDNTCRCILDIFESTLPTALITWKPPSPNNENAKYKLFSALLIKCVVQLELIQTIDNVVFYPATSRKEDQENLALAQADMLNGKPSELGVQGGGDQQKEEQGMYCSLTTKHLLQLVGCLLKSHRFAKTFNSNDEQRQLWKSGFYCSMKSETGLL-NQEIQSLACALRILFKMYSDEAHRNDWATVETLLVEVACEALEYFLALSNEMHIDAWTPILLLLLTRILKMSDSRFAVHASSCYPLLCEVMCFDLKPELRSVLRRFFLRIGPVFRI 1664          
BLAST of GF14256 vs. nr
Match: gi|194761140|ref|XP_001962790.1| (uncharacterized protein Dana_GF14256 [Drosophila ananassae] >gi|190616487|gb|EDV32011.1| uncharacterized protein Dana_GF14256 [Drosophila ananassae])

HSP 1 Score: 1239.56 bits (3206), Expect = 0.000e+0
Identity = 678/1397 (48.53%), Postives = 901/1397 (64.50%), Query Frame = 0
Query:    1 MFLEKAIESLLQEKDVKRPQYQELREACEVALADLRA--LPASNTSDGQ---CSVLP--EIEPGQLIHEERILHPFELACRQSKNTKLVIFSLDSLQKLVAYGHLRFSHTRLPLNHNPGHPSLEDRLVSSVAHAFSGPQTDEQVQLQVLKALLTILTSANVRVHDNSLLLSVRTCYNIYLASRNIINQTTAKATLSQILNYVFSTMESASAVSLPSHEP--------------------ETASEAVHVVRPILL-----------------------------------DLADHLVAREFLAPEVYRNMMQRDAYLVFRSLCKLSMKPLPEGAPDPKSHELRSKILSLELLLSILQNSGDAFQHNDIFVSAIKSYLCVALSQNGVSNVSEVFELSLALFISLLTKYRVHLKPQIEIFFKEICLNILEASSSSFEQKWMVVQGIGNICHDAQIVIDIFVNYDCDLEAANIFERLVDDLAKLAQGKQAYEIGATPNQLMQMRIRCLECLCFILKCMVEWSKELYVNPHQAEKDAVPETPEATDED---------------------------PAHLERVKQRKFVLEYGIKLFTHKPVKGIKYLVENHIVSKGDKEVAQFLHKECNRLDKTAIGEFLGELE--NKEAMYHYVDEINFAELDFVSALRVFLGNFRLPGEAQKIDRLMEKFASRYVECNPTQKLFKSADAAYVLAYSVIMLTTDLHSTSVKKKMTKEEFLRNNKGINDSE-DIPPEYLSKIYDAIASNEIKMKGTS------LANNKVIITDLKQRTLLWNRESESISQTAGALMESASSRTDAFTSAKRLDHVKPMFKLIWSPLLATFSVGLQSTDDMVVVQLCLEGIRCCIRLANIFNFSLERDAFIQALARFTLLTENSNVNEMRSKNIEAIKTLISVAHTDGNYLQHSWLDIMKCISQLEYAQLLGSS------GHGPDGLESHARPSTLVMDVTSEANSQGIIVAVDRIFTGSKNLDGNAIVHFFNALCDVSKEEIGSKQSVRMYSLMKLVEISYYNMERIRLEWSRIWHVVGNHFNLVGCKDDQNVSFFAVDSLKQLSLKFLEKGELQNFHFQKDFLRPFEYIMKNNKSPQIRDMVVRCLSQMIQSQSQNIKSGWKNIFATFGLAAGDDVESIVALSHQTTQRIVTKLISDNKFALKESFQD---CLSCVINKSKLDIRTRSLTIL---FDIVKQHGQSFEES---------------WWRDLYQVLFR-------------------IFEVGK--------------FDM-----NDLSLRDH----REWIDTTCNHALYAMTDVFSEFYEVLVGVVLTDLLSQYQWCIQQPNEQLSKSAISCFQTLVATNQAKMEADTFGLIISFLADIIKDT 1230
            MF+ +A+E +L +KD++R  + +L+++C+ AL  ++A  + A   ++G    C+ LP  + +   +I+ E    PFELAC+ S++ ++V+ +LD LQKL+AYGHL  S   +  + NPGH  L DR+V ++   FSGPQTDE VQLQ++KALLT++TS +V +H+ +LL +VRTCY+IYL+S+N++NQTTA+ATL+Q+LN +F+ ME+      P   P                    ET+++A  V+   LL                                   D  +     + +    + +++Q+DA+LVFR+LCKLSMKPLPEG PDPKSHELRSK+LSL LLL ILQN+G  F+ N++F+ AIK YLCVALS NGVS V EVFELSL++F++LL+ ++VHLK QIE+FFKEI LNILEA+SSSFE KWMV+Q +  IC DAQ V+DI+VNYDCD  AAN+FERLV+DL+K+AQG+QA E+GA P Q   MRIR LECL  ILKCMVEWSK+LYVNP+        ++P ATD D                           P  LE  K RK V+E GI+LF  KP KG+++L E  ++    +++A++LH++  RLDKT IG +LGE +  +KE M  Y+D  +F +L+ V+ALR+ L  FRLPGEAQKIDRLMEKFASRY ECNP  +LF+SAD  YVLA+S+IMLTTDLHS  VK KMTKE++++ N+GI+DS+ D+P EYLS IYD IA +EIKMK  S          +  IT+ K+R LLWN E E IS TA  LM+S S     FTSAK L+HV+PMFK+ W+P LA FSVGLQ  DD  +  LCL+GIRC IR+A IF+ SLERDA++QALARFTLL  NS +NEM++KNI+ IKTLI VAHTDGNYL  SWLDI+KCISQLE AQL+G+             L+    PS  V +   E +SQ ++VAVDRIFTGS  LDG+AIV F  ALC VS +E+  +Q  RM+SL K+VEISYYNMERIRL+WSRIW V+G HFN VGC +++ ++FFA+DSL+QLS+KF+EKGE  NF FQKDFLRPFE+IMK N SP IRDMVVRC++QM+ SQ+ NI+SGWKNIF+ F LAAGD  E IV L+ QTT +I+  L       + +SFQD   CLS        D    S+ ++      V +  Q F E                W R  + +LF                    +FE+ K              F++     +++ L +H     EW+ TTCNHALYA+ DVF+++++VL  ++L +L +Q  WC+QQ NEQL++S  +C + LV +N  K    T+      + DI   T
Sbjct:   12 MFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLPKNDAASIINAETYFLPFELACK-SRSPRIVVTALDCLQKLIAYGHLTGS---IQDSANPGH-LLIDRIVVTIYGCFSGPQTDEGVQLQIIKALLTVVTSQHVEIHEFTLLQAVRTCYDIYLSSKNLVNQTTARATLTQMLNVIFARMENQVYEMPPPPNPINGSIHSEDCNGSTGGGSSDETSADADEVIASELLAEIITAAYNEAMKDVESGGEGEPVANGNESSHSDHDSVELHSENDAVVTAKFTHILQKDAFLVFRALCKLSMKPLPEGHPDPKSHELRSKVLSLHLLLLILQNAGPVFRSNEMFIMAIKQYLCVALSNNGVSLVPEVFELSLSIFVALLSNFKVHLKRQIEVFFKEIFLNILEANSSSFEHKWMVIQALTRICADAQSVVDIYVNYDCDFSAANLFERLVNDLSKIAQGRQALELGANPIQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNMPAPALQVQSPTATDHDQPDTTIQTTHSGSSHSLNSNQEQFQDLPEALEERKMRKEVMETGIELFNRKPQKGVQFLQEKQLLGSTCQDIARWLHED-ERLDKTVIGNYLGENDEHSKEVMCAYIDAFDFRQLEVVAALRILLEEFRLPGEAQKIDRLMEKFASRYCECNPQNQLFQSADTVYVLAFSIIMLTTDLHSPQVKHKMTKEQYIKMNRGISDSKADLPEEYLSSIYDEIAEHEIKMKNNSGLLQPKPTGKQAFITE-KRRKLLWNMEMEVISLTATNLMQSVSHVKSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIATLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCISQLELAQLIGTGVRPQFLSGAQTTLKDSLNPS--VKEHIGETSSQSVVVAVDRIFTGSMRLDGDAIVDFVKALCHVSVDELQQQQP-RMFSLQKIVEISYYNMERIRLQWSRIWQVLGEHFNTVGCNNNEEIAFFALDSLRQLSMKFMEKGEFSNFRFQKDFLRPFEHIMKKNASPAIRDMVVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDHEEPIVELAFQTTGKIIGDLYQRQFAIMVDSFQDAVKCLSEFATARFADTSMESIRLVRTCAQCVHEAPQLFAEHAGMENDASVAEEDRVWVRGWFPMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPNWWKDLFNVIFRIFDNMKLPEHVTEKSEWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCVQQSNEQLARSGTNCLENLVISNGFKFNESTWDKTCQCILDIFNAT 1398          
The following BLAST results are available for this feature:
BLAST of GF14256 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 9
Match NameE-valueIdentityDescription
EMLSAG000000115680.000e+051.78supercontig:LSalAtl2s:LSalAtl2s800:154479:160331:1... [more]
EMLSAG000000116561.075e-4145.65supercontig:LSalAtl2s:LSalAtl2s80:773194:804518:1 ... [more]
EMLSAG000000062884.393e-3925.71supercontig:LSalAtl2s:LSalAtl2s342:169461:179750:-... [more]
EMLSAG000000019233.386e-3439.79supercontig:LSalAtl2s:LSalAtl2s1330:63988:71857:1 ... [more]
EMLSAG000000032164.440e-3133.91supercontig:LSalAtl2s:LSalAtl2s177:439240:441692:1... [more]
EMLSAG000000054381.739e-1840.68supercontig:LSalAtl2s:LSalAtl2s292:598840:621289:-... [more]
EMLSAG000000083911.262e-1528.49supercontig:LSalAtl2s:LSalAtl2s512:629114:630490:1... [more]
EMLSAG000000067792.188e-927.15supercontig:LSalAtl2s:LSalAtl2s37:479446:498428:1 ... [more]
EMLSAG000000060024.676e-924.38supercontig:LSalAtl2s:LSalAtl2s3255:3251:10476:1 g... [more]
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BLAST of GF14256 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|408407575|sp|A2A5R2.1|BIG2_MOUSE3.842e-4650.75RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|81865497|sp|Q7TSU1.1|BIG2_RAT2.619e-4650.57RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|146329988|sp|Q9Y6D5.3|BIG2_HUMAN7.432e-4650.49RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|13123969|sp|O46382.1|BIG1_BOVIN7.023e-4751.20RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|408387574|sp|G3X9K3.1|BIG1_MOUSE3.235e-4750.85RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|116241267|sp|Q9Y6D6.2|BIG1_HUMAN7.266e-4750.85RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|408407574|sp|D4A631.1|BIG1_RAT4.469e-4750.76RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|449061809|sp|F4JSZ5.1|BIG1_ARATH0.000e+036.16RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|449061867|sp|F4IXW2.2|BIG5_ARATH0.000e+035.85RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]
gi|75264111|sp|Q9LPC5.1|BIG3_ARATH2.644e-1337.46RecName: Full=Brefeldin A-inhibited guanine nucleo... [more]

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BLAST of GF14256 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1012973208|gb|KYQ47635.1|0.000e+046.40Brefeldin A-inhibited guanine nucleotide-exchange ... [more]
gi|1070611469|ref|XP_018403612.1|0.000e+044.57PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|985412762|ref|XP_015371907.1|0.000e+044.10PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|1028720451|ref|XP_016663116.1|0.000e+043.85PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|1230208436|ref|XP_022179292.1|0.000e+044.04brefeldin A-inhibited guanine nucleotide-exchange ... [more]
gi|954560261|ref|XP_014603898.1|0.000e+043.76PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|1189084159|ref|XP_020810363.1|0.000e+048.50brefeldin A-inhibited guanine nucleotide-exchange ... [more]
gi|1036705398|ref|XP_017037331.1|0.000e+048.46PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|972195788|ref|XP_015179780.1|0.000e+043.60PREDICTED: brefeldin A-inhibited guanine nucleotid... [more]
gi|194761140|ref|XP_001962790.1|0.000e+048.53uncharacterized protein Dana_GF14256 [Drosophila a... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold722_size106786supercontigscaffold722_size106786:5335..9894 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
NoteP:regulation of ARF protein signal transduction
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
maker-scaffold722_size106786-snap-gene-0.21-mRNA-1maker-scaffold722_size106786-snap-gene-0.21-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold722_size106786:5335..9894+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>maker-scaffold722_size106786-snap-gene-0.21 ID=maker-scaffold722_size106786-snap-gene-0.21|Name=GF14256|organism=Tigriopus kingsejongensis|type=gene|length=4560bp|location=Sequence derived from alignment at scaffold722_size106786:5335..9894+ (Tigriopus kingsejongensis)
ATGTTCCTGGAGAAGGCCATTGAGAGCCTCCTGCAGGAGAAGGATGTGAA GCGGCCGCAGTATCAGGAGTTGCGCGAGGCCTGTGAGGTCGCACTGGCCG ACCTCCGGGCCTTGCCGGCCTCGAACACGAGCGACGGCCAATGCTCCGTC CTGCCCGAGATCGAACCCGGGCAGCTCATCCACGAGGAGCGCATCCTCCA CCCCTTCGAGCTGGCCTGTCGCCAGAGCAAGAACACCAAGCTGGTCATCT TCTCCCTGGACAGCCTCCAGAAGCTGGTGGCCTACGGACATTTGCGCTTC TCCCACACCCGTTTGCCGCTCAACCACAACCCGGGCCATCCCTCGCTCGA GGATCGGCTGGTGAGCTCGGTGGCCCACGCCTTCAGCGGGCCGCAGACGG ACGAGCAGGTTCAGTTGCAAGTCCTCAAGGCCCTGCTCACCATATTGACT TCGGCCAATGTGCGGGTGCACGACAATAGCCTACTCCTGTCCGTCCGGAC GTGCTACAACATCTACCTGGCCTCGCGGAACATCATCAATCAGACCACGG CCAAGGCCACGCTCAGCCAGATCCTCAACTACGTGTTCAGCACCATGGAG TCGGCCTCGGCCGTGAGTTTGCCCTCCCACGAGCCCGAGACGGCGTCCGA GGCCGTCCACGTGGTCCGGCCCATCCTCTTGGACCTGGCCGACCACCTGG TGGCCCGCGAGTTCCTGGCCCCCGAGGTCTACCGCAACATGATGCAGCGC GATGCTTACCTCGTTTTCCGCTCCCTCTGCAAGCTCTCCATGAAACCCCT GCCCGAGGGGGCACCGGATCCCAAGTCCCACGAGCTCCGCTCCAAGATCC TGAGTCTGGAGCTGCTCTTGAGCATCCTGCAGAACAGCGGCGACGCCTTC CAGCACAATGACATCTTCGTGTCGGCCATCAAGTCCTACCTCTGCGTGGC TCTCTCCCAGAATGGCGTGTCCAACGTGAGCGAGGTGTTCGAGCTGTCCC TGGCCCTCTTCATATCGCTCCTGACCAAGTATCGAGTGCACCTGAAGCCT CAGATCGAGATCTTCTTCAAAGAGATCTGTCTCAACATCCTCGAGGCCTC GAGCTCGTCCTTTGAACAGAAGTGGATGGTCGTCCAGGGCATCGGCAACA TTTGCCACGACGCCCAGATCGTCATTGACATCTTTGTCAACTACGACTGC GACCTCGAGGCCGCCAACATCTTCGAGCGGCTGGTGGACGATCTGGCCAA GCTGGCCCAGGGCAAGCAGGCCTACGAGATCGGGGCCACGCCCAATCAGT TGATGCAGATGCGGATCCGCTGCCTCGAGTGCCTCTGCTTCATCCTCAAG TGCATGGTCGAGTGGAGCAAGGAGCTCTACGTCAACCCGCACCAGGCCGA GAAGGACGCCGTGCCCGAGACGCCTGAAGCCACCGATGAGGACCCCGCGC ACTTGGAGCGAGTCAAGCAGCGCAAGTTCGTGCTCGAGTACGGCATCAAG CTGTTCACTCACAAGCCGGTCAAAGGCATCAAGTATCTGGTGGAGAATCA CATCGTGAGCAAGGGCGACAAGGAGGTGGCTCAGTTCCTCCACAAGGAGT GCAATCGCCTAGACAAGACGGCCATCGGCGAGTTCTTGGGCGAGCTCGAG AACAAGGAAGCCATGTACCACTACGTGGACGAGATCAACTTTGCCGAGCT GGACTTTGTTTCGGCCTTGCGGGTGTTCCTCGGCAACTTTCGCCTCCCCG GCGAGGCCCAGAAGATCGATCGTCTCATGGAGAAGTTCGCCTCGCGCTAC GTGGAGTGCAATCCCACCCAGAAGCTCTTCAAGTCGGCCGACGCCGCCTA CGTGCTCGCCTACTCCGTGATCATGCTCACCACTGATTTGCACAGCACGA GCGTGAAGAAGAAGATGACCAAGGAGGAGTTTCTCCGGAACAACAAGGGC ATCAACGATAGCGAGGACATCCCGCCCGAGTATCTGTCCAAGATCTACGA CGCCATCGCCTCCAACGAGATCAAGATGAAGGGCACATCCCTGGCCAACA ACAAGGTGATCATCACAGATCTGAAGCAGAGAACCCTGCTCTGGAACCGC GAGAGCGAGTCCATCTCCCAGACGGCCGGAGCGCTCATGGAGTCGGCCAG TAGCCGGACCGATGCCTTCACCTCGGCCAAGCGGCTGGACCACGTCAAGC CCATGTTCAAGCTGATCTGGTCGCCGCTCTTGGCGACCTTCAGTGTCGGC CTCCAGTCCACCGACGACATGGTGGTGGTCCAGCTCTGCCTCGAGGGCAT CCGATGCTGCATCCGCCTGGCCAACATATTCAACTTCTCCCTGGAGCGGG ACGCCTTCATCCAAGCCTTGGCTCGCTTCACGCTCCTCACCGAGAACTCG AACGTGAACGAGATGCGCTCGAAGAACATCGAGGCCATCAAGACCCTGAT CTCGGTGGCTCACACGGACGGCAACTACTTGCAGCACTCGTGGCTGGACA TCATGAAGTGCATCTCCCAGTTGGAGTACGCCCAATTGCTGGGCTCCTCC GGCCACGGCCCCGACGGGCTGGAGTCCCACGCCCGCCCCAGTACGCTGGT GATGGACGTGACTAGTGAGGCCAATAGTCAGGGCATCATCGTGGCCGTGG ACCGGATCTTCACCGGCTCCAAGAACTTGGACGGAAACGCCATCGTCCAC TTCTTCAACGCTCTCTGCGACGTGTCCAAGGAGGAGATCGGCTCGAAGCA AAGCGTGAGGATGTATTCGCTCATGAAGCTGGTCGAGATCAGCTACTACA ACATGGAGCGTATCCGACTCGAGTGGTCCCGGATCTGGCACGTGGTGGGC AATCACTTCAACCTGGTCGGCTGTAAGGACGATCAGAACGTGTCGTTCTT CGCCGTGGACTCGCTCAAGCAGCTCTCCCTCAAGTTCTTGGAGAAGGGTG AGCTGCAGAATTTCCACTTTCAAAAGGACTTCCTCCGCCCCTTCGAGTAC ATCATGAAGAACAACAAGTCCCCCCAGATCCGCGACATGGTGGTCCGCTG CCTCTCCCAGATGATCCAGAGCCAGAGTCAGAACATCAAGTCGGGCTGGA AGAACATCTTTGCCACCTTCGGCCTGGCGGCCGGCGACGACGTCGAGTCC ATTGTGGCACTGAGCCACCAGACCACCCAGCGGATCGTGACCAAGTTGAT CAGCGACAACAAGTTCGCCCTGAAGGAGTCCTTCCAGGACTGCGTCAAAT GTCTCTCAGAGTTTGCCTGCAATCAGATGCTCCAGGACGTGGCCATGGAG TCGCTCCAATTGATCCGCAAGTGCGCCGAGTTCGTCCACAACCACCAGGA CCAGTTCGAGACTCTGGCCGGCCCGCTGCTCTCGTCCTCTAGTAGCTCTC GCGGCACGAACGGCGAGGCGGCGGATGAGGCGGCCCTGGCTCGGAATAAC GTCTGGATCCGGGGCTGGTTTCCCGTCCTCTTTGAGCTGTCCTGCGTGAT CAACAAGAGCAAGCTGGACATCCGGACCCGGAGTCTCACTATCCTCTTCG ACATTGTCAAGCAGCACGGGCAGTCCTTTGAGGAGAGCTGGTGGCGGGAC CTCTACCAAGTGCTCTTCCGGATCTTCGAGGTGGGCAAGTTCGATATGAA CGACCTCTCGCTCCGGGATCACCGCGAGTGGATCGACACCACGTGCAACC ACGCCCTGTACGCCATGACGGACGTGTTCAGCGAGTTCTACGAGGTCCTC GTCGGCGTGGTCCTCACCGACCTGCTTAGCCAGTATCAGTGGTGCATCCA ACAGCCCAATGAGCAGTTGTCCAAGTCAGCCATCTCGTGCTTCCAGACAC TGGTGGCCACCAACCAGGCCAAGATGGAGGCAGACACGTTCGGGCTCATC ATCTCCTTCCTCGCCGACATCATCAAGGACACGTCGGTGATCGTGTACAA GGAGGAAGACATCGTCTTTGGACGGGTCCACGTCCATCTGGACGTCCTGG AGGTGTCCATGAACGTACTCACGTCCACTGCCTATGGTCAGACCATCGAG GGCCCGCCCTGGTTCGAAGTGCTCATGCCCGACCACTTCCAGGTGCTCAA GAAGCTCCTCCGGAGCCTGGAGCAGTCGTTCAACGAGTCCAAAAGCGTGC TCGAGGACACGCCCAATAGCTCTCGGAAGCGCGATCTCTCCATCCTGGTC AAGCAGGAGACCAAGGCCGCCGAGTGTCTGCTCACCATCCTCATCGAGCT GTGCACCTCGAATGTGGGCAACGAGTTTCAAAAGTCGTTGGAGAAGTATT TCGTGAGCTTCCTAGTCAACGTTCTCGGCTACTTCCTCTCGATGACGACC AAGACCCAGCAGGAGCATTGGGATCGCATGCTCGCCTCGATCTCGGAGCA GATCCTCAACTTCCAAGAGCCCATGTTCATCTCGGCCATGTCCTCGCTCT ACAACTTGATTTGTGATATCTTGAGCATCGGCGTGTCCAAGGAGTTCCGC GGAGTTCTGTGCCAAATGCTGAAGCGGATCGGATCCCTGTACGACATTCC AAGACTCTAG
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Synonyms
The feature 'GF14256' has the following synonyms
Synonym
Tk04290
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