BLAST of vacuolar protein-sorting vs. L. salmonis genes
Match: EMLSAG00000001293 (supercontig:LSalAtl2s:LSalAtl2s1214:59320:63000:-1 gene:EMLSAG00000001293 transcript:EMLSAT00000001293 description:"maker-LSalAtl2s1214-augustus-gene-0.47")

HSP 1 Score: 469.159 bits (1206), Expect = 8.389e-156
Identity = 217/398 (54.52%), Postives = 301/398 (75.63%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVG----FFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDL--HDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPH 409
            C   L R +NMLL F++DL SIS EI++LQQQSV MNL+L+NRQSV  ELSQFV+++V+PE MI +++ +PVT+  FL+QL  LE+KI FLKEQSF +A+SC+DV+D V+KLK   + KIR+FLLE+I+QF+KPLAN HIPQN MLKF FY+KF+MAN+R++A E++++Y DTMSKVLFSYFKSYSGRL KLQ+ ++ASR+DL+G  +S      G    F  K  +K+KA+VFS+G R +V+   LE PIIVPHH  QK ++KY +E LFRSEQ+  +EN CRE++F+  FFMVE +    +++F+ ++G+T   + K ++   QESYD I+LFLC+HL++ +R  C  R ++ +D+YWD++Q   W+R   V ++N+QSI DCDP RLK +D RPH
Sbjct:   75 CDGILMRMENMLLGFKTDLSSISQEIISLQQQSVDMNLKLKNRQSVHEELSQFVTDLVIPENMIKILMHTPVTDVDFLEQLKNLEKKINFLKEQSFSDARSCSDVKDTVEKLKIACITKIREFLLERINQFKKPLANNHIPQNMMLKFGFYYKFVMANNRDVAKEIKDEYVDTMSKVLFSYFKSYSGRLLKLQYGDLASREDLLGADDSKLGRFSGPSFIFSSKPNLKNKATVFSLGSRDDVLSSELEMPIIVPHHANQKNDTKYPFEFLFRSEQYALVENGCREYLFLVDFFMVESDTTPANEMFNAVMGRTFQHIIKQIDHYTQESYDCIALFLCIHLIYKYRILCHKRAVTAMDRYWDTVQGLFWSRLTRVFEMNVQSIRDCDPSRLKLLDLRPH 472          

HSP 2 Score: 151.369 bits (381), Expect = 1.382e-38
Identity = 71/140 (50.71%), Postives = 97/140 (69.29%), Query Frame = 0

Query:  479 ERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEV 618
            ER+  D KESD F++ +   I E++ L+L   FG ++ F+   EP ++N+    + +E + V  I+  FN  WKK+LD INSE+ +SFPN KNGT+ILQQ LT FVQYYHRF+KI+S P FS+   R+ LINVHQLMVE+
Sbjct:  474 ERINSDSKESDIFQEQMNIHISEYITLVLSPKFGAIINFLNEAEPLVENNKTDKLLQEEKRVLCIIEKFNSEWKKSLDDINSEVFASFPNLKNGTSILQQILTNFVQYYHRFHKIISLPIFSNSSCRTNLINVHQLMVEM 613          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|81870466|sp|O55166.2|VPS52_RAT (RecName: Full=Vacuolar protein sorting-associated protein 52 homolog; AltName: Full=SAC2 suppressor of actin mutations 2-like protein)

HSP 1 Score: 618.616 bits (1594), Expect = 0.000e+0
Identity = 284/613 (46.33%), Postives = 422/613 (68.84%), Query Frame = 0

Query:   14 KMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEA-LEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            ++  C   L+R + ML  FQSDL SIS EI  LQ+QS  MN++L+NRQ+VRG+L + V  +VVP  ++T ILE+PVTEP+FL+QL  L+ K   ++EQ  R   +C DVR V+ +L+ KA+ KIR+F+L+KI  FRKP+ NY IPQ  +LK+ F+++FL+ N+R  A EVR++Y +T+SK+  SY++SY GRL K+Q+EE+A +DDL+G VE T +   GFF K +++S+ ++F++G R  VI  A LEAPI+VPH   Q+ E +Y +E LFRS+ +  L+N+CRE++FI +FF+V G   HDLFH ++G+T  +  K +E  + + YDAI++FLC+H++  FR     R +  LD+YW+ + A LW RF+ ++++N+QS+   DP RL  +D RPH I RRYAE S+  +S+++  PNE   +LL  LQ EVE F+L++ + F  RK QL+FLINNYDM+L VL ER   D KE +SF+  L  R  EF+E +L   FG L+ F+K  E  ++      +  E   V+ ++R F   WK +++S++ +++ SF NF+NGT+I+Q  LTQ +Q YHRF++++S PQ  + P R++LIN+H LMVE+KK K NF
Sbjct:  115 QITACDAVLERMEQMLGAFQSDLSSISCEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVREQEARGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQTALLKYRFFYQFLLGNERATAKEVRDEYVETLSKIYLSYYRSYVGRLMKVQYEEVAEKDDLMG-VEDTAKK--GFFSKPSLRSRNTIFTLGTRGAVISPAELEAPILVPH-TAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGPAAHDLFHAVMGRTLSMTLKHLESYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTLQLLGQLQVEVENFVLRVAAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAERLRGEEARVTQLIRGFGSSWKASVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVELKKHKPNF 723          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|81876888|sp|Q8C754.1|VPS52_MOUSE (RecName: Full=Vacuolar protein sorting-associated protein 52 homolog)

HSP 1 Score: 617.461 bits (1591), Expect = 0.000e+0
Identity = 283/613 (46.17%), Postives = 422/613 (68.84%), Query Frame = 0

Query:   14 KMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEA-LEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            ++  C   L+R + ML  FQSDL SISSEI  LQ+QS  MN++L+NRQ+VRG+L + V  +VVP  ++T ILE+PVTEP+FL+QL  L+ K   ++EQ      +C DVR V+ +L+ KA+ KIR+F+L+KI  FRKP+ NY IPQ  +LK+ F+++FL+ N+R  A E+R++Y +T+SK+  SY++SY GRL K+Q+EE+A +DDL+G VE T +   GFF K +++S+ ++F++G R  VI  A LEAPI+VPH   Q+ E +Y +E LFRS+ +  L+N+CRE++FI +FF+V G   HDLFH ++G+T  +  K +E  + + YDAI++FLC+H++  FR     R +  LD+YW+ + A LW RF+ ++++N+QS+   DP RL  +D RPH I RRYAE S+  +S+++  PNE   +LL  LQ EVE F+L++ + F  RK QL+FLINNYDM+L VL ER   D KE +SF+  L  R  EF+E +L   FG L+ F+K  E  ++      +  E   V+ ++R F   WK +++S++ +++ SF NF+NGT+I+Q  LTQ +Q YHRF++++S PQ  + P R++LIN+H LMVE+KK K NF
Sbjct:  115 QITACDAVLERMEQMLGAFQSDLSSISSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVREQEAMGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQAALLKYRFFYQFLLGNERATAKEIRDEYVETLSKIYLSYYRSYVGRLMKVQYEEVAEKDDLMG-VEDTAKK--GFFSKPSLRSRNTIFTLGTRGTVISPAELEAPILVPH-TAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFIVSGPAAHDLFHAVMGRTLSMTLKHLESYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTLQLLGQLQVEVENFVLRVAAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAERLRGEEARVTQLIRGFGSSWKASVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVELKKHKPNF 723          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|74750887|sp|Q8N1B4.1|VPS52_HUMAN (RecName: Full=Vacuolar protein sorting-associated protein 52 homolog; AltName: Full=SAC2 suppressor of actin mutations 2-like protein)

HSP 1 Score: 617.076 bits (1590), Expect = 0.000e+0
Identity = 282/613 (46.00%), Postives = 423/613 (69.00%), Query Frame = 0

Query:   14 KMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEA-LEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            ++  C   L+R + ML  FQSDL SISSEI  LQ+QS  MN++L+NRQ+VRG+L + V  +VVP  ++T ILE+PVTEP+FL+QL  L+ K   ++EQ  R   +C DVR V+ +L+ KA+ KIR+F+L+KI  FRKP+ NY IPQ  +LK+ F+++FL+ N+R  A E+R++Y +T+SK+  SY++SY GRL K+Q+EE+A +DDL+G VE T +   GFF K +++S+ ++F++G R +VI    LEAPI+VPH   Q+ E +Y +E LFRS+ +  L+N+CRE++FI +FF+V G   HDLFH ++G+T  +  K ++  + + YDAI++FLC+H++  FR     R +  LD+YW+ + A LW RF+ ++++N+QS+   DP RL  +D RPH I RRYAE S+  +S+++  PNE   +LL  LQ EVE F+L++ + F  RK QL+FLINNYDM+L VL ER   D KE +SF+  L  R  EF+E +L   FG L+ F+K  E  ++      +  E   V+ ++R F   WK +++S++ +++ SF NF+NGT+I+Q  LTQ +Q YHRF++++S PQ  + P R++LIN+H LMVE+KK K NF
Sbjct:  115 QITACDAVLERMEQMLGAFQSDLSSISSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVREQEARGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQTALLKYRFFYQFLLGNERATAKEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMG-VEDTAKK--GFFSKPSLRSRNTIFTLGTRGSVISPTELEAPILVPH-TAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGPAAHDLFHAVMGRTLSMTLKHLDSYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTMQLLGQLQVEVENFVLRVAAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAERLRGEEARVTQLIRGFGSSWKSSVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVELKKHKPNF 723          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|75042795|sp|Q5TJF0.1|VPS52_CANFA (RecName: Full=Vacuolar protein sorting-associated protein 52 homolog)

HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0
Identity = 281/613 (45.84%), Postives = 423/613 (69.00%), Query Frame = 0

Query:   14 KMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEA-LEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            ++  C   L+R + ML  FQSDL SISSEI  LQ+QS  MN++L+NRQ+VRG+L + V  ++VP  +I  ILE+PVTEP+FL+QL  L+ K   ++EQ  R   +C DVR ++ +L+ KA+ KIR+F+L+KI  FRKP+ NY IPQ  +LK+ F+++FL+ N+R  A E+R++Y +T+SK+  SY++SY GRL K+Q+EE+A +DDL+G VE T +   GFF K +++S+ ++F++G R +VI    LEAPI+VPH   Q+ E +Y +E LFRS+ +  L+N+CRE++FI +FF+V G+  HDLFH ++G+T  +  K +E  + + YDAI++FLC+H++  FR     R +  LD+YW+ + A LW RF+ ++++N+QS+   DP RL  +D RPH I RRYAE S+  +S+++  PNE   +LL  LQ EVE F+L++ + F  RK QL+FLINNYDM+L VL ER   D KE +SF+  L  R  EF+E +L   FG L+ F+K  E  ++      +  E   V+ ++R F   WK +++S++ +++ SF NF+NGT+I+Q  LTQ +Q YHRF++++S PQ  + P R++LIN+H LMVE+KK K NF
Sbjct:  115 QITACDAVLERMEQMLGAFQSDLSSISSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLIVPSALIMAILEAPVTEPRFLEQLQELDAKAAAVREQEARGTAACADVRGILDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQTALLKYRFFYQFLLGNERATAKEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMG-VEDTAKK--GFFSKPSLRSRNTIFTLGTRGSVISPTELEAPILVPH-TAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGSAAHDLFHAVMGRTLGMTLKHLESYLTDCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTVPNERTMQLLGQLQVEVENFVLRVAAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAERLRGEEARVTQLIRGFGSSWKSSVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVELKKHKPNF 723          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|460425447|sp|G5EFV8.2|VPS52_CAEEL (RecName: Full=Vacuolar protein sorting-associated protein 52 homolog)

HSP 1 Score: 420.239 bits (1079), Expect = 2.200e-137
Identity = 224/624 (35.90%), Postives = 382/624 (61.22%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVG--FFGKT-TMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRL---KTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARL---LSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  +++ C    +R Q  L +FQ +LGSI  ++  LQ QS  ++ +L+NRQ VR ELSQFV ++ V + M+  I ++   +  FL+ L  L  KIT + ++   +A +  D   +++ LK KA+ K+R++LL+K+ QFRKPL+NY + Q+ +LK  F+++FL+ +D   A E++++Y DT+SK+ F+YFK+Y+ RL KL  +++A+++D +G+++  + + +G  F  K   +++KA+VFSIG+R  ++ +     +IVPH   Q  +S Y +E LFRS Q  F+++   E++FI  FF+V  ++  +L ++ + +   ++ K+ E  +  S+DAISL LC+ L   F      R++  +  YW+++ + LW R   VM  + +S+   D  +L    ++D RPH I RRYAEL++  L + + S  E  A++   L + +  +E  + +M+++ + +KN+ +FLINNYD+ILS++        K      +     I +FVE MLE H G +++F+   E  +       + R   +V +++ +FN +W+ A+DSINSE +  F NF  GTTILQ   T++VQY +RF KI+SH  F+ +P  SQL+NVHQ+M+E+K+FK  +
Sbjct:   80 LHNQLVHCDNVFERLQATLYSFQDNLGSIGQDMKNLQLQSHHIHQELENRQKVRVELSQFVDDIAVSQTMMKTINDTDANDRGFLEALHELHHKITLILQRGNGDAVAVNDTMPILEGLKLKAVVKVREWLLQKMFQFRKPLSNYQVFQHQLLKCRFFYEFLLHHDLISAKELQDEYIDTISKMFFTYFKAYATRLFKLAMKDVATKEDALGSIDFAKPAGLGAIFSSKQHVVRNKATVFSIGQRHQILSDDFLGALIVPHAATQNHQS-YQFEALFRSIQLAFVDHYSHEYLFITDFFLVSNDEAIELHNKAMARAMSVVLKSCEEQIALSWDAISLHLCICLCDKFTEVLAEREVPEVSDYWNTVTSFLWTRLNLVMSQHYESVKSVDLKKLMHSGSLDARPHFIVRRYAELTSAHLMIAKASGKEMGAKMEAVLENSEDSIEQLLTRMSAMQQTQKNKHVFLINNYDLILSIIDNEESKHTKIYAIVHELEQKSIDDFVEEMLEPHIGYMIKFVNECESLIVQGHTQLLVRYNDKVGTVVANFNAKWRPAVDSINSECIQLFTNFSLGTTILQTIFTKYVQYINRFTKILSHDVFAKNPVCSQLVNVHQVMLEIKRFKPAY 702          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|75172681|sp|Q9FVV6.1|VP52B_ARATH (RecName: Full=Vacuolar protein sorting-associated protein 52 B; AltName: Full=ARE1-like protein P2)

HSP 1 Score: 333.569 bits (854), Expect = 4.253e-104
Identity = 204/614 (33.22%), Postives = 346/614 (56.35%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLK-EQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTM-LKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFG--KTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELR--EVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C+  L + + +L  FQ ++GSISS+I  LQ+ S+ M L+L+NR+    +L++FV + +VP +MI VI++  V E +++  L  L +K+ F++ +Q+ + +K+  DV   ++KL+ KA++K+ DF+++K+   RKP  N  I Q ++ LK+ +   FL  + +E+  +VR  Y DTM+KVL ++F+SY     KLQ + IA+ +DL+G      RS  G F   K  +K++ +VF++GER  +I E ++ P ++PH + + +  KY YEVLFRS     ++ A  E++F   FF  +      +F++I      ++ + ++  +   YDAI L L + ++   +     R+I  LD Y D +  SLW RF+ V  L+I S+ + +   +   D  PH I RRYAE +A F+ L+    +  +   L  L+  V+  ILK+  +F   K Q++FLINNYDM ++VL E      K    F++ L +    F E +L  HF ++++F+K        D  P ++R +   EV  +++DF  RWK A++ ++ +I++ F NF  G  IL   +TQ + YY R    +      S  +R  ++N   +M E+KK+K  F
Sbjct:  105 CNCILSQMETLLSGFQEEIGSISSDIKILQENSMDMGLRLKNRRVTESKLAKFVEDFIVPPKMIHVIVDGEVNE-EYIKTLGILSKKLKFVETDQAVKSSKALKDVEPELEKLRQKAISKVYDFIVQKLIALRKPKTNIQILQQSVFLKYKYIISFLKEHGKEVFMDVRAAYIDTMNKVLSAHFQSYIQAFEKLQLD-IATSNDLIGV---DTRST-GLFSRSKEPLKNRCAVFALGERIQIIKE-IDQPALIPH-IAEASSLKYPYEVLFRSLHKLLMDTATSEYIFCEDFFGEQS-----IFYEIFAGPFSVIYEHLDSVLSSCYDAIGLLLMIRIIHHHQLIMSRRRIPCLDSYLDKVNISLWPRFKTVFDLHIGSLRNANINTIWEDDVHPHYIMRRYAEFTASFIHLNVEYGDGQLDINLERLRMAVDSLILKLAKLFPRPKQQMVFLINNYDMTIAVLKEAEPEGGKIQMHFEELLKSNTSLFAEELLVEHFSDMIKFVK---SRANEDSSPNLERSITVAEVEPLVKDFGSRWKTAIELMHKDIITCFSNFLCGMDILIAGMTQLLLYYTRLEDCIKKIDGGSALNRD-IVNYQSIMFEIKKYKKTF 701          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|75166438|sp|Q94KD3.1|VP52A_ARATH (RecName: Full=Vacuolar protein sorting-associated protein 52 A; Short=AtVPS52; AltName: Full=ARE1-like protein POK; AltName: Full=Protein POKY POLLEN TUBE; AltName: Full=Protein T-DNA TRANSMISSION DEFECT 8)

HSP 1 Score: 332.028 bits (850), Expect = 2.415e-103
Identity = 199/612 (32.52%), Postives = 346/612 (56.54%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLK-EQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHI-PQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKT--TMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C   L + + +L  FQ ++GSISS+I  LQ++S+ M L+L+NR+    +L++FV +++VP +MI VI++  V E +++  L  L +K+ F++ +Q+ + +K+  DV   ++KL+ KA++K+ DF+++K+   RKP  N  I  Q+ +LK+ +   FL  + +E+  +VR  Y DTM+KVL ++F++Y   L KLQ  +IA+  DL+G    T     G F +    +K++++VF++G+R  +I + ++ P ++P H+ + +  KY YEVLFRS     ++ A  E++F + FF  E      +F++I      ++ +     +   +DAI L L + ++   +     R+I  LD Y D +  SLW RF+ V   ++ S+ D +   L   D  PH + RRYAE +A F+ L+    +  +   L  L+  V+  ILK+  +F   K Q++FLINNYDM ++VL E      K    F++ L +    FVE +L  HF +L++F+K    +  +   P     + EV  +++DF  RWK A++ ++ +I++SF NF  G  IL+  LTQ + YY R    +      S  +R  L+++  +M E++K+   F
Sbjct:  111 CDSILSQMETLLSGFQEEIGSISSDIKILQEKSMDMGLRLKNRRVAESKLAKFVEDIIVPPKMIDVIVDGEVNE-EYMKTLEILSKKLKFVEADQAVKSSKALKDVEPELEKLRQKAISKVYDFIVQKLIALRKPKTNIQILQQSVLLKYKYIISFLKEHGKEVFMDVRAAYIDTMNKVLSAHFRAYIQALEKLQL-DIATAYDLIGVETRT----TGLFSRAREPLKNRSAVFALGDRIKIIKD-IDQPALIP-HIAEASSLKYPYEVLFRSLHKLLMDTATSEYMFCDDFFGEES-----IFYEIFAGPFSVIDEHFNPVLSNCFDAIGLMLMIRIIHHHQLIMSRRRIPCLDSYLDKVNISLWPRFKMVFDSHLSSLRDANIKTLWEDDVHPHYVMRRYAEFTASFIHLNVEYGDGQLDINLERLRMAVDGLILKLAKLFPRPKQQIVFLINNYDMTIAVLKEAGPEGGKIQMHFEEMLKSNTSLFVEELLVEHFSDLIKFVKN-RASEDSSLNPERSITIAEVEPLVKDFGSRWKTAIELMDKDIITSFSNFLCGMDILRAALTQLLLYYTRLTDCIKKIDGGSALNR-DLVSIQSIMYEIRKYSKTF 707          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|74625872|sp|Q9UST3.1|VPS52_SCHPO (RecName: Full=Vacuolar protein sorting-associated protein 52)

HSP 1 Score: 117.087 bits (292), Expect = 1.507e-26
Identity = 111/463 (23.97%), Postives = 209/463 (45.14%), Query Frame = 0

Query:   32 FQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQS--------FREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLAN-YHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFG--KTTMKSKAS-VFSIGERANVIDEALEAPIIVPHHVQQKAESKYSY-EVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCD-PLRLKTVDQRPHLICRRYAEL--SAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLG 478
            FQ DL   +S+I  + ++S ++ + L N ++V   L   +   ++P ++I  I    +  P +     ALE+  +FL+           F   +   D + +V KL+ KA+ +IR++++  I  FR+   + + I ++ ++    Y+ FL   +R++A E++  Y +TM+     +F+ YS  L K+          L G     +    G F   K   +S  + + S+  R    D          HH+    ES   Y E ++ S +    E+   E+ F+ ++F +  +    +F  I  KT    +K +   +  S D I +   +          Q   +  ++ +++S+   LW RFQ VM ++ +S+   +  ++ + +  RPH + +R AEL  S   LS++ V   E +AR  S L Q+    +  +      ++ Q  FL NNY +I +VL 
Sbjct:   37 FQVDLDVATSDIYKVSERSNKIQVNLNNLKAVESALGAEIDGAILPPDLIKTISTGDMDHPSWNS---ALEKLTSFLEGGEDDSSLGNMFNSLQINKDQKKLVDKLRDKAIERIRNYIVVTIKMFRQAFVDVFPIRKHRLIANKNYYLFLFKFNRKLALELQRAYINTMNWFYLYHFEQYSRFLDKVHV--------LKGETIRVEEDRKGLFNLSKGAQQSYGNQMLSVNLRPQDFDMNSVLTASTMHHI----ESSPQYIERVYCSWELVLTEHVSSEYAFLLEYFNLSKDQQATVFAAIFEKTLHFSRKYITGLISSSIDCIGILKSIRFTQKLALQAQTNIVPVIEPHYNSMILFLWPRFQAVMDMHCESLRKTNLSVKPEEITSRPHPLSQRVAELLYSLSMLSVNVVEA-EPVARSASRLAQDYVSMLQNLCKTVNDKRRQSRFLSNNYTLISTVLS 483          

BLAST of vacuolar protein-sorting vs. SwissProt
Match: gi|2507120|sp|P39904.2|VPS52_YEAST (RecName: Full=Vacuolar protein sorting-associated protein 52; AltName: Full=Suppressor of actin mutation protein 2)

HSP 1 Score: 83.5741 bits (205), Expect = 1.550e-15
Identity = 112/513 (21.83%), Postives = 213/513 (41.52%), Query Frame = 0

Query:   29 LLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFL--KEQSFREAKSCTDVRDVVKKLKFKAMAKIRDF-------------LLEK-----ISQFRKPLANYHIP----QNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGK---------TTMKSKA-------------SVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGND---LHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTL-DKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQR------------------PHLICRRYAELSAGFLSL---HEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNY 470
            L  F + L + + ++  ++++S ++   L+   +    +S  V+++++P E+I  I++  + E        + +  ITF+  KE+ + + +S    +D  K  +  AM   +DF             +LE+     IS+ +   ++  +P    QN +LK    F F+  N+  +A E+R+ YC TM      YF  Y   L  LQF++I S+  L   + +T  SV GF            TT  S A               F I +R N++ +  E   ++   + +   +K   E+ F++     L+N   E+ F+  FF + G++   ++ L  QI   T        +  +Q +YD   + + + +    +   + R I ++ D + +     LW RFQ ++         C+ LR   +                     PH +  ++ +  + FL+L   H+ S +E    L + + +    F   MT   +  K+   FL  NY
Sbjct:   81 LTEFTNRLSNYTQDLDFIKKKSNELQSLLEYNSTKLAHISPMVNDLMIPPELIDDIIKGKINE--------SWQDNITFIADKEEIYNKYRSNNLDQDN-KDAENSAMLAPKDFDKLCQLLDILKNVILERSKRLIISKIKTLRSHNPVPSQRIQNKLLKVQKIFPFIRDNNLSLALELRQAYCYTMKWYYREYFSRYIRSLTILQFQQIDSQFALGNGLSTT--SVSGFNNSPSLFFSNYLTTSASNAFYNKLPVTDEKIDKYFQIKKRLNILTQ--EDNTVMVSQIAENNTTKNYIEIGFKNLNLAILDNCTVEYHFLKDFFAMNGDNFEEINGLLEQIFQPTFDEATTYTQQLIQYNYDIFGVLISIRVANQLQFESERRGIPSMFDSFLNGQLIQLWPRFQQLVDF------QCESLRKAAITTNVAKYAGNSSTSNSSPLTSPHELTVQFGKFLSSFLTLAITHKQSIDERSEPLYNSIIRLRNDFETVMTKCSKKTKSPERFLATNY 574          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|926623977|ref|XP_013777425.1| (vacuolar protein sorting-associated protein 52 homolog [Limulus polyphemus] >gi|926623979|ref|XP_013777426.1| vacuolar protein sorting-associated protein 52 homolog [Limulus polyphemus])

HSP 1 Score: 699.123 bits (1803), Expect = 0.000e+0
Identity = 323/608 (53.12%), Postives = 452/608 (74.34%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C   L+R + ML TFQ DLGSIS EIL LQ+QSV MN++L+NRQ++RGELSQFV +MVVP+ +I  ILE+PV E  FL+Q+  L+ KI+F+KEQ+F+E  SC DVRD++ KLKFKA++KIR++LL++I  FRKP+ NY + Q+TMLKF F+++FLM ++RE+A E+R++Y DTMSK+ FSYFKSY+ RL KLQFEE A +DDL+G  ++ ++S+  F  K ++K+KA++F++G+R NV+   LE+PI+VPH   QK E+KY++E LFRS+Q+  ++NACRE++F+ +FFMV G    DLFH ++GKT  +  K V++ +Q+ YD+I++FLC+H++  F+     R +  LDKYW+++    W RF+Y++QLNIQS+ DCDP +  + D RPH I RRYAE SA  + ++E  P+E + RLL+ LQ EVE FIL+M + F GRK QL+FLINNYDM+LSVL ER R D KES+SFK+ L  R  E+VE +L  HFG L+ F+K  E  L+      + +E ++V  ++R FN  WK +LD IN +I+ SF NFKNGT ILQ  LTQ +QYYHRF+K++S   F + P RS+LIN+H LMVEVKK+K+NF
Sbjct:   73 CDGILERMEQMLRTFQEDLGSISHEILTLQEQSVSMNIRLKNRQAIRGELSQFVDDMVVPDVLINHILETPVVEKDFLEQIYVLDHKISFVKEQAFKEVHSCQDVRDILDKLKFKALSKIREYLLQRIYSFRKPMTNYQVTQDTMLKFKFFYQFLMTHEREVAREIRDEYVDTMSKIYFSYFKSYTTRLMKLQFEETADKDDLMGVEDTARKSI--FSTKPSLKNKATIFTLGDRGNVLTTDLESPILVPH-TAQKNETKYTFESLFRSQQYALVDNACREYLFLTEFFMVSGTGAQDLFHAVIGKTLTMFMKHVDVYIQDCYDSIAVFLCIHIIHRFQLLMHKRAVPALDKYWEALLQLFWPRFEYILQLNIQSVRDCDPQKFGSFDLRPHYITRRYAEFSAALVGINENFPSEQVTRLLAALQGEVENFILRMAAEFPGRKEQLVFLINNYDMMLSVLLERTREDSKESESFKELLNARTHEYVEEILSPHFGGLISFVKMCENYLERGQSEHLKKEEKKVPQLVRSFNSGWKGSLDDINKDIMKSFTNFKNGTNILQVALTQLIQYYHRFHKVLSQNPFKNLPIRSELINIHHLMVEVKKYKANF 677          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|919061969|ref|XP_013412634.1| (vacuolar protein sorting-associated protein 52 homolog isoform X1 [Lingula anatina])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 322/616 (52.27%), Postives = 440/616 (71.43%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFG-KTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+  + ML +FQ+DL SISSEI  LQ+QS+ MN++L+NRQ+VRGELSQFV EMVVPE MI  IL+SPVTE +FL+QL  L  KI F+KEQSF+E++SC DV+D+++KLK KA+AKIR+FLL+KI QF+KP+ NY +PQN MLKF F+++FL+AN+R +A EVR++Y D MSK+ +SYFK YS RL KLQFEE+A +DDL+G  ++ ++   GFF  K  +K+++++F++G R  V+   LEAPIIVPH   QK++ KY++E LFRS+ +  L+N CRE++FI  FFMV G+   DLF+ ++GKT     K ++L   E +D+I++FLC+H+++ F+     R +  LDKYW+++   LW RF+ ++ +NIQS+ DCDP RL  +D RPH I RRYAE SA  + L+E  P+E + RLL+ LQ EVE F+L+M + F  RK QL+FLINNYDM+LSV+ ER   D KES+SFK+ L  R  EFVE +L  HFG L+ F+K  E  L+  +   +  + R V  I+R FN  WKKA++  N + + SF NFKNGT ILQ  LTQ +QYYHRF KIMS   F S   RS+LIN+H +MVEVKK K  F
Sbjct:   91 LHTQITACDSILETMEQMLQSFQTDLSSISSEIQTLQEQSIAMNIKLKNRQAVRGELSQFVDEMVVPESMINHILDSPVTEREFLEQLHELNHKINFVKEQSFKESRSCMDVKDILEKLKIKAIAKIREFLLQKIYQFKKPMTNYQVPQNAMLKFRFFYEFLLANERHVAKEVRDEYLDIMSKIYYSYFKGYSSRLMKLQFEEVADKDDLMGAEDTAKK---GFFSSKPALKNRSTIFTVGNRGAVLTSELEAPIIVPH-AAQKSDKKYTFESLFRSQHYALLDNCCREYLFIVDFFMVSGSAAQDLFNAVMGKTMSSFLKQMDLFTNECFDSIAIFLCIHIIYRFQVAMHKRSVPALDKYWETLLEILWPRFEQILHMNIQSVKDCDPSRLGPIDTRPHYIVRRYAEFSAAIVGLNESFPSEKVNRLLALLQGEVENFVLRMAAEFSARKEQLIFLINNYDMMLSVIMERTHEDSKESESFKELLAARTQEFVEEILAPHFGGLMSFVKECEVQLEKGNTEQLKHQERRVQQIVRGFNSEWKKAIELTNQDAMRSFTNFKNGTQILQGALTQLIQYYHRFTKIMSQAPFKSMAIRSELINIHHVMVEVKKHKPTF 702          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|1325267653|ref|XP_023342298.1| (vacuolar protein sorting-associated protein 52 homolog [Eurytemora affinis])

HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0
Identity = 334/608 (54.93%), Postives = 453/608 (74.51%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C   L R + +L  FQ+DLGSIS+EIL+LQQQSV+MNL+L+NRQ+VRGELSQFV +MVV E+MI+ ILE PVTE QF +QL  L  KI FL EQSFR+AKSC DV+DV++KLK KA+AKIR++ + KI+QF+KPL NY IPQ+ +LKF FYF+FL A +RE+A E+R++Y DTMSK+LFSYFKSY+GRL+KLQFEE ASRDDL+G  E++ +   GFF K ++K+K++VFS+G R +V++  LEAPIIVPH  Q K E KY +E++FRS Q+  ++N CREF+F+++ F+V+G +  DLF+ + GKT  +L K  +  VQ+SYD+I++FLC+HL+  ++  C  R +  LDKYWD++   LW R   +++LN QS+ DCD  R+K  D RPH I RRYAE SAG + ++E  P+E++ +LL+ LQ+EVE  IL++   F  RK+QLLFLINNYD+I+S++ ER + + KESD+F++ L +R  EFVE +L+ HFG L+++I   E  L+  D   + +E ++VS I+  F   WKK+LD IN E LSSFPN K GT ILQQTLT  +QYYHRF ++MS    S  P  S LIN+HQLMVEVKK+K NF
Sbjct:   82 CDGILARMEGLLTAFQTDLGSISNEILSLQQQSVEMNLRLKNRQAVRGELSQFVDDMVVTEQMISTILEVPVTETQFYEQLHILNHKIAFLNEQSFRDAKSCVDVKDVIEKLKIKAVAKIREYFMLKINQFKKPLTNYQIPQSGILKFKFYFQFLQAVNREVAKEIRDEYTDTMSKILFSYFKSYTGRLSKLQFEESASRDDLLGAEETSSK---GFFFKPSLKNKSTVFSVGCRDDVLNSQLEAPIIVPH-AQLKNEMKYPFEMIFRSVQYTLVDNGCREFLFLSELFLVDGQNAQDLFNFVFGKTLQILIKFTDTYVQDSYDSIAVFLCIHLVQRYQLLCHKRCVPGLDKYWDTMNGILWPRLTQLLELNTQSVRDCDVSRIKPTDVRPHFITRRYAEYSAGIVGINESFPHEHMHKLLAYLQEEVESLILRLAGTFPARKDQLLFLINNYDVIMSIILERTKEETKESDAFREQLRSRSEEFVEQILQAHFGYLIQWINEAERKLEKGDIDGLKQEEKKVSQIISTFGAEWKKSLDRINGETLSSFPNLKLGTGILQQTLTTLLQYYHRFTRLMSVAPLSQTPAASNLINIHQLMVEVKKYKPNF 685          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|1316183401|ref|XP_023237376.1| (vacuolar protein sorting-associated protein 52 homolog [Centruroides sculpturatus])

HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0
Identity = 323/608 (53.12%), Postives = 455/608 (74.84%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C   L+R + ML +FQ+DLGSIS EIL LQQQSV MN++L+NRQ+VRGELSQFV EM+VP+ MI  ILE+PVTE  FL+QL  L+ KI+F+KEQ F++A+SC DV+D+++KLKFKA+ KIR++LL+KI  FRKP+ NY +PQN MLK  FY++FLM ++REIA E+R++Y DTM K+ +SYFKSYS RL KLQFEE+A +DDL+G  ++ +R +  F  K ++K+++++F++G R +V+   LE+PIIVPH   QK E+KY++E LFRS+ F  ++N+ RE++F+++FFM       DLF+ ++G+T     K +++ VQ+ YD+I++FLC+HL+  ++     R +  LDKYW+++   LW RF+Y++QLNIQSI DCDP +L ++D RPH I RRYAE SA  + ++E  P+E +ARLL+ LQ EVE FIL+M + F  RK QL+FLINNYDM+LSVL ER + D KES+SFK+ L  R  E+VE +L  HFG ++ F+K  E  L+  +   + +E ++V+S++R FN  WKKA+D IN +I+ SF NFKNGT ILQ TLTQ +QYYHRF K++S   F + P RS+L+N+H LMVEVKK+KS+F
Sbjct:   90 CDEILERMEQMLRSFQNDLGSISQEILTLQQQSVTMNIRLKNRQAVRGELSQFVDEMIVPDIMINHILETPVTEKGFLEQLFILDHKISFVKEQGFKDARSCQDVKDILEKLKFKAIVKIREYLLQKIYAFRKPMTNYQVPQNAMLKHKFYYQFLMTHEREIAREIRDEYIDTMMKIYYSYFKSYSSRLMKLQFEEMADKDDLMGVEDTAKRGI--FSSKPSLKNRSTIFTLGNRGSVLTTDLESPIIVPH-AAQKNETKYTFESLFRSQHFALVDNSSREYLFLSEFFMAANTAAQDLFNSVMGRTLTTFLKQIDVYVQDCYDSIAIFLCIHLIHRYQLLMHKRAVPALDKYWEALLQLLWPRFEYILQLNIQSIRDCDPQKLGSIDMRPHYITRRYAEFSAAIVGINESYPSECVARLLAALQGEVENFILRMAAEFPERKEQLIFLINNYDMMLSVLLERTKDDSKESESFKELLNARTQEYVEEVLSPHFGGMISFVKECEKYLERGNVDILKKEEKKVASLIRSFNSGWKKAIDDINQDIMRSFTNFKNGTNILQATLTQLIQYYHRFQKVLSQTPFKNLPIRSELLNIHHLMVEVKKYKSSF 694          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|1009597818|ref|XP_015927404.1| (vacuolar protein sorting-associated protein 52 homolog [Parasteatoda tepidariorum] >gi|1009597821|ref|XP_015927406.1| vacuolar protein sorting-associated protein 52 homolog [Parasteatoda tepidariorum] >gi|1009597823|ref|XP_015927407.1| vacuolar protein sorting-associated protein 52 homolog [Parasteatoda tepidariorum])

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 316/615 (51.38%), Postives = 449/615 (73.01%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+R + ML  FQ+DLGSIS EIL LQQQSV MN++L+NRQ++RGELSQFV +M+V + +I  I+ESPVT+ +FL+ L  L+ KI F+KEQSF++AK C DV+DV++KLK KA+ KIR+FLL+KI  FRKP+ NY IPQN +LK+ F+++FLM ++R+IA EVRE+Y DTMSK+LFSYFK Y+ RL KLQFEE+A RDDL+G  ++ +R +  F  K ++K++++VF++G R N++   LE+ IIVPH  Q K E+KY++E LFRS+QF  ++NACRE++FI++FFMV      +LF+ ++ KT  +  K  +  VQ+ YD+I+LFLC+H++  ++     R +  LDKYW+++    W RF++++QLNI+SI DCDP +   +D+RPH I RRYAE SA  + ++E  P+E +ARLL+ LQ EVE FIL+M + F   K+QL+F INNYDM+L VL ER + D +ES+SFKD L  RI E+VE +L  HFG ++ F+K  E  L+      +  E  +V S+++ FN  WKKA++ IN++I+++F NFKNGT ILQ  LTQ +QYYHRF KI++   F S P RS+L+N+H +MVE KKFK+NF
Sbjct:   97 LHNQITACDEILERMEQMLRLFQTDLGSISKEILTLQQQSVSMNIRLKNRQAIRGELSQFVDDMIVQDNLIQHIIESPVTDKEFLEDLQILDHKIAFVKEQSFKDAKCCQDVKDVLEKLKIKAITKIREFLLQKIYSFRKPMTNYQIPQNALLKYKFFYQFLMTHERDIAREVREEYLDTMSKILFSYFKMYTSRLMKLQFEELADRDDLMGVEDTAKRGI--FSSKPSLKNRSTVFTLGNRGNILTTELESQIIVPHSAQ-KNETKYTFESLFRSQQFALVDNACREYLFISEFFMVSSKSAGELFNSVMLKTLSMFLKNTQQYVQDCYDSIALFLCIHVIHRYQVLMHKRSVPALDKYWENLLQMFWPRFEHILQLNIESIRDCDPQKFSNIDKRPHYITRRYAEFSAAIVGINENFPSERVARLLAALQVEVENFILRMAAEFPDHKDQLIFQINNYDMMLGVLLERTKEDSRESESFKDLLNARILEYVEEVLSPHFGGMIVFVKDCEKYLERGQLENLKVEAGKVPSLVKSFNNGWKKAMEEINADIMTTFTNFKNGTNILQAALTQLIQYYHRFQKIVNQHPFKSLPIRSELLNIHNIMVEAKKFKTNF 708          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|957848835|ref|XP_014674938.1| (PREDICTED: vacuolar protein sorting-associated protein 52 homolog isoform X3 [Priapulus caudatus])

HSP 1 Score: 662.144 bits (1707), Expect = 0.000e+0
Identity = 311/615 (50.57%), Postives = 441/615 (71.71%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+R + ML  FQ+DL SIS EI  LQ+QS+ MN++L+NRQSVRGELSQFV E+VVP+ MI  IL+ PVTE  FL+QL  L  KI F+KEQSF ++KS  DV+DV++KLK KA+AKIR+FLL +I QFRKP+ NY +PQN MLKF F+++FL+AN+R +A EVR++Y DTMSK+ +SYF+SYS R+ KLQ+E++A ++DL+G  ++ +R +  F  K  +K ++++F++  R N++   LEA IIVPH   QK++ K++YE LFRS  +  L+N CRE++F+  FFMV     HDLF+ ++GKT     K +E  V E YD+I+LFLC+H+++ ++     R +  LDKYW+++Q  LW RF+Y+M LNIQSI D DP +L ++D RPH I RRYAE SA  + L+E  P+E + RLL+ LQ EVE FIL+M + F  R+ QL+FLINNYDM+L+V+ ER   D KES+SFK+ L  R  EFVE +L  +FG ++ F+K  E  ++      + ++ ++V+ I+R FN  WKKA+D +N ++++SF NFKNGT+ILQ  LTQ +QYYHRF K++S+P +     R++LIN+H +MVEVKK K NF
Sbjct:   88 LHNQITACDSILERMEQMLSGFQTDLSSISDEIQTLQEQSIAMNVKLKNRQSVRGELSQFVHEIVVPDFMINTILDVPVTERNFLEQLHELNHKINFVKEQSFHDSKSALDVKDVLEKLKIKAIAKIREFLLLRIYQFRKPMTNYQVPQNAMLKFRFFYEFLLANERHVAKEVRDEYVDTMSKIYYSYFRSYSNRIMKLQYEDVADKEDLMGVEDTAKRGL--FSSKPQLKQRSTIFTLSSRGNILTSELEASIIVPH-AAQKSDRKFAYEALFRSMHYALLDNCCREYLFLTDFFMVASAAAHDLFYMVMGKTLGTFLKDLEYCVNECYDSIALFLCIHIVYRYQVIMHKRGVPALDKYWETVQEMLWPRFEYIMHLNIQSIKDTDPSKLGSIDVRPHYITRRYAEFSAAIVGLNESFPSERVNRLLATLQGEVENFILRMAAEFPQRREQLIFLINNYDMMLAVIMERTTDDSKESESFKELLNARTEEFVEEILAPYFGGMITFVKESENMIERGQADRLKKDEKKVTMIVRSFNSDWKKAIDDLNQQVMTSFTNFKNGTSILQGALTQLIQYYHRFQKVLSNPPYKVLAVRNELINIHHVMVEVKKHKPNF 699          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|957848833|ref|XP_014674937.1| (PREDICTED: vacuolar protein sorting-associated protein 52 homolog isoform X2 [Priapulus caudatus])

HSP 1 Score: 662.144 bits (1707), Expect = 0.000e+0
Identity = 311/615 (50.57%), Postives = 441/615 (71.71%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+R + ML  FQ+DL SIS EI  LQ+QS+ MN++L+NRQSVRGELSQFV E+VVP+ MI  IL+ PVTE  FL+QL  L  KI F+KEQSF ++KS  DV+DV++KLK KA+AKIR+FLL +I QFRKP+ NY +PQN MLKF F+++FL+AN+R +A EVR++Y DTMSK+ +SYF+SYS R+ KLQ+E++A ++DL+G  ++ +R +  F  K  +K ++++F++  R N++   LEA IIVPH   QK++ K++YE LFRS  +  L+N CRE++F+  FFMV     HDLF+ ++GKT     K +E  V E YD+I+LFLC+H+++ ++     R +  LDKYW+++Q  LW RF+Y+M LNIQSI D DP +L ++D RPH I RRYAE SA  + L+E  P+E + RLL+ LQ EVE FIL+M + F  R+ QL+FLINNYDM+L+V+ ER   D KES+SFK+ L  R  EFVE +L  +FG ++ F+K  E  ++      + ++ ++V+ I+R FN  WKKA+D +N ++++SF NFKNGT+ILQ  LTQ +QYYHRF K++S+P +     R++LIN+H +MVEVKK K NF
Sbjct:   89 LHNQITACDSILERMEQMLSGFQTDLSSISDEIQTLQEQSIAMNVKLKNRQSVRGELSQFVHEIVVPDFMINTILDVPVTERNFLEQLHELNHKINFVKEQSFHDSKSALDVKDVLEKLKIKAIAKIREFLLLRIYQFRKPMTNYQVPQNAMLKFRFFYEFLLANERHVAKEVRDEYVDTMSKIYYSYFRSYSNRIMKLQYEDVADKEDLMGVEDTAKRGL--FSSKPQLKQRSTIFTLSSRGNILTSELEASIIVPH-AAQKSDRKFAYEALFRSMHYALLDNCCREYLFLTDFFMVASAAAHDLFYMVMGKTLGTFLKDLEYCVNECYDSIALFLCIHIVYRYQVIMHKRGVPALDKYWETVQEMLWPRFEYIMHLNIQSIKDTDPSKLGSIDVRPHYITRRYAEFSAAIVGLNESFPSERVNRLLATLQGEVENFILRMAAEFPQRREQLIFLINNYDMMLAVIMERTTDDSKESESFKELLNARTEEFVEEILAPYFGGMITFVKESENMIERGQADRLKKDEKKVTMIVRSFNSDWKKAIDDLNQQVMTSFTNFKNGTSILQGALTQLIQYYHRFQKVLSNPPYKVLAVRNELINIHHVMVEVKKHKPNF 700          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|957848831|ref|XP_014674936.1| (PREDICTED: vacuolar protein sorting-associated protein 52 homolog isoform X1 [Priapulus caudatus])

HSP 1 Score: 661.759 bits (1706), Expect = 0.000e+0
Identity = 311/615 (50.57%), Postives = 441/615 (71.71%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+R + ML  FQ+DL SIS EI  LQ+QS+ MN++L+NRQSVRGELSQFV E+VVP+ MI  IL+ PVTE  FL+QL  L  KI F+KEQSF ++KS  DV+DV++KLK KA+AKIR+FLL +I QFRKP+ NY +PQN MLKF F+++FL+AN+R +A EVR++Y DTMSK+ +SYF+SYS R+ KLQ+E++A ++DL+G  ++ +R +  F  K  +K ++++F++  R N++   LEA IIVPH   QK++ K++YE LFRS  +  L+N CRE++F+  FFMV     HDLF+ ++GKT     K +E  V E YD+I+LFLC+H+++ ++     R +  LDKYW+++Q  LW RF+Y+M LNIQSI D DP +L ++D RPH I RRYAE SA  + L+E  P+E + RLL+ LQ EVE FIL+M + F  R+ QL+FLINNYDM+L+V+ ER   D KES+SFK+ L  R  EFVE +L  +FG ++ F+K  E  ++      + ++ ++V+ I+R FN  WKKA+D +N ++++SF NFKNGT+ILQ  LTQ +QYYHRF K++S+P +     R++LIN+H +MVEVKK K NF
Sbjct:   96 LHNQITACDSILERMEQMLSGFQTDLSSISDEIQTLQEQSIAMNVKLKNRQSVRGELSQFVHEIVVPDFMINTILDVPVTERNFLEQLHELNHKINFVKEQSFHDSKSALDVKDVLEKLKIKAIAKIREFLLLRIYQFRKPMTNYQVPQNAMLKFRFFYEFLLANERHVAKEVRDEYVDTMSKIYYSYFRSYSNRIMKLQYEDVADKEDLMGVEDTAKRGL--FSSKPQLKQRSTIFTLSSRGNILTSELEASIIVPH-AAQKSDRKFAYEALFRSMHYALLDNCCREYLFLTDFFMVASAAAHDLFYMVMGKTLGTFLKDLEYCVNECYDSIALFLCIHIVYRYQVIMHKRGVPALDKYWETVQEMLWPRFEYIMHLNIQSIKDTDPSKLGSIDVRPHYITRRYAEFSAAIVGLNESFPSERVNRLLATLQGEVENFILRMAAEFPQRREQLIFLINNYDMMLAVIMERTTDDSKESESFKELLNARTEEFVEEILAPYFGGMITFVKESENMIERGQADRLKKDEKKVTMIVRSFNSDWKKAIDDLNQQVMTSFTNFKNGTSILQGALTQLIQYYHRFQKVLSNPPYKVLAVRNELINIHHVMVEVKKHKPNF 707          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|1229144116|ref|XP_022084333.1| (vacuolar protein sorting-associated protein 52 homolog [Acanthaster planci])

HSP 1 Score: 660.603 bits (1703), Expect = 0.000e+0
Identity = 310/608 (50.99%), Postives = 437/608 (71.88%), Query Frame = 0

Query:   18 CSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFGKTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            C   L+R + ML  FQSDLGS+S+EI  LQ QS+ MN++L+NRQ+VRGELSQFV EM V E MI  IL++PVTE QFL+QL  L  KI+F+KEQSF++A SC DV+D++ KLK KA+AKIR+FLL+KI+Q RKP+ NY + QNTMLKF F+F+FL++N+R++A EVR+DY DTMSKV +SYFK Y  RL KLQ++E+A ++DL+G  ++ +R   GFF K ++K++++VF++G R ++I   LE+PII+PH   QK E+K+++E L RS+ +  L+N CRE++F+  FFMV G+   DLF+ I+GKT  +  K +++   E +D+I+++LC+H++  +R     R +  LDKYW+++   LW RF+Y+++LNI SI + DP ++ ++D RPH I RRYAE S   +SL+E  P+E + RLL+ L  +VE FIL+M + F  RK QL+FLINNYDM+L+V+ ER   D +ESDSF+  L  R  EFVE +L  HFG ++ F+K  E  L+      +  + R V  ++RDF   WK AL+ IN +++ SF NFKNGTTILQ  LTQ +QYYHRF K++S P F +   R +LIN+H LMVEVKKFK  F
Sbjct:  102 CDTILERMEEMLNGFQSDLGSLSAEIQTLQNQSIAMNIKLKNRQAVRGELSQFVDEMAVQETMINHILDTPVTERQFLEQLHELNHKISFVKEQSFKDALSCKDVQDILDKLKLKAIAKIREFLLQKINQMRKPMTNYQLQQNTMLKFRFFFEFLLSNERQVAKEVRDDYVDTMSKVYYSYFKGYISRLMKLQYDEVADKEDLMGVEDTAKR---GFFSKPSLKNRSTVFTLGNRGSIITTNLESPIIIPH-AAQKDEAKHTFESLCRSQHYALLDNCCREYLFVCDFFMVSGSSAQDLFNAIMGKTLSMFLKHMDMYTNECFDSIAIYLCIHIILRYRMLMHKRAVPALDKYWETLLEMLWPRFEYILELNIHSIREADPQKMGSIDIRPHYITRRYAEFSGAIVSLNETFPDERVNRLLARLMADVENFILRMAAEFPERKEQLIFLINNYDMMLNVINERTAEDSRESDSFRQLLEARTQEFVEEVLLPHFGGMIMFVKDAESRLERGQADQMKSQERRVEQLVRDFGSSWKPALEMINQDVMRSFTNFKNGTTILQNCLTQLIQYYHRFQKVLSQPPFKNMAVRGELINIHHLMVEVKKFKPAF 705          

BLAST of vacuolar protein-sorting vs. nr
Match: gi|524877133|ref|XP_005095273.1| (PREDICTED: vacuolar protein sorting-associated protein 52 homolog isoform X3 [Aplysia californica])

HSP 1 Score: 659.448 bits (1700), Expect = 0.000e+0
Identity = 318/616 (51.62%), Postives = 436/616 (70.78%), Query Frame = 0

Query:   11 LTPKMILCSFGLQRCQNMLLTFQSDLGSISSEILALQQQSVQMNLQLQNRQSVRGELSQFVSEMVVPEEMITVILESPVTEPQFLDQLVALEQKITFLKEQSFREAKSCTDVRDVVKKLKFKAMAKIRDFLLEKISQFRKPLANYHIPQNTMLKFSFYFKFLMANDREIASEVREDYCDTMSKVLFSYFKSYSGRLAKLQFEEIASRDDLVGTVESTQRSVVGFFG-KTTMKSKASVFSIGERANVIDEALEAPIIVPHHVQQKAESKYSYEVLFRSEQFCFLENACREFVFINQFFMVEGNDLHDLFHQILGKTEILLKKAVELSVQESYDAISLFLCLHLMFAFRAHCQLRKISTLDKYWDSIQASLWNRFQYVMQLNIQSIHDCDPLRLKTVDQRPHLICRRYAELSAGFLSLHEVSPNENIARLLSDLQQEVEYFILKMTSVFEGRKNQLLFLINNYDMILSVLGERVRGDCKESDSFKDFLGTRIGEFVELMLETHFGELLRFIKRVEPALQNDDEPAIDRELREVSSILRDFNVRWKKALDSINSEILSSFPNFKNGTTILQQTLTQFVQYYHRFYKIMSHPQFSSHPDRSQLINVHQLMVEVKKFKSNF 625
            L  ++  C   L+R + ML  FQ DL SIS+EI +LQ QS+ MN++L+NRQ++RGELSQ+V EMV+PE MIT ILESPVTE  F +QL  L  KI F+KEQSFR+A++C DV+D+++KLK KA+ KIR+FLL+KI  FRKP+ NY IPQNT+LKF  + +FLMAN+R +A EVR++Y +TMSK+ +SYFK YS RL KLQFEE A+RDDL+G VE T +   GFF  K  MK++++VF++G R +V+   LEA +IVPH    K E++YS+E +FRS  F  ++N CRE++F++ FFMV G    +LF  I+GKT  +  K VE SVQE YD+I +FL  H+++ +++    R I  LD+YW++I ++LW RF+Y++ +NIQS+ + DP +L  +D RPH + RR+AE SA  + ++   PNE + R+L  LQ EVE FILKM + F  RK QL+FLINNYDM+LSVL ER   D KE++ ++D L  R  EFVE +L  HFG L+ F+K  E  L+      + +E + V  I+R FN  WK+AL+SIN E++ +F NFKNGT ILQ  LT  +QYYHRF KI+S P F +   + +LIN+H +MVEVKK+K  F
Sbjct:   91 LHKQIAACDTILERMEQMLNGFQGDLSSISTEIQSLQDQSIAMNIKLKNRQAIRGELSQYVDEMVIPESMITSILESPVTEDDFKEQLHELNHKINFVKEQSFRDARACLDVKDILEKLKIKAIGKIREFLLQKIYAFRKPMTNYQIPQNTLLKFRIFNEFLMANERHVAKEVRDEYVETMSKIYYSYFKGYSSRLTKLQFEEAATRDDLMG-VEDTAKK--GFFSSKPAMKNRSTVFTLGNRGDVLTTELEAAVIVPH-AAAKTETRYSFESIFRSLHFALMDNCCREYLFVSDFFMVSGTGAQNLFENIMGKTLTMFLKLVEDSVQECYDSIGIFLSAHVVYRYQSIMAKRSIPALDRYWETILSNLWPRFEYIVSMNIQSVRERDPNKLGHIDNRPHYVTRRFAEFSAAIVGINVSYPNERVNRVLGQLQAEVENFILKMAAEFPQRKEQLIFLINNYDMMLSVLVERTSDDSKEAEMYRDLLAARTQEFVEEILAPHFGGLVTFVKDAEVCLERGQSDRLQQEEKRVQQIVRGFNSDWKRALESINQEVMRAFTNFKNGTQILQGALTLLIQYYHRFQKILSQPVFRNLQIKHELINIHHVMVEVKKYKPTF 702