voltage-gated calcium channel alpha 1 subunit, maker-scaffold81_size397536-snap-gene-2.22 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000010843 (supercontig:LSalAtl2s:LSalAtl2s721:13130:108134:1 gene:EMLSAG00000010843 transcript:EMLSAT00000010843 description:"maker-LSalAtl2s721-augustus-gene-1.27") HSP 1 Score: 1754.18 bits (4542), Expect = 0.000e+0 Identity = 922/1198 (76.96%), Postives = 1004/1198 (83.81%), Query Frame = 0
Query: 16 RYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQK---PRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDD--PPEDGDDDEEGKD-------GGEDGETKIPMDQYYP-DDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDH-EEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLT----------NEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKISPTNSMDYAA-QNSFRKMPDEDFPSDAANIQMRPVKLANGGSQDHSRP 1188
RYWK L NL+ SL NSI +I SLL+LLFLF+ IFALLG QVFG ++ + K R NFD+F QSL++VFQILTGEDWNVVMYDGIQAFGGV SIGI+SSLYFIILFICGNYILLNVFLAIAVDNLADADALEDE + E+ PPEDG EG D G D E ++ +DQ Y +DYDQY ++ D + GQ ++ D+++ RM SESP +PIP+ SAF++F +NQFRI+CH+ICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFT+EVCLKVI+YGF+LHPGAFCRSGFNLLDLLVVAVSLISF+FSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVA KTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMC+D+SKMT ETC+G+FI YKDGDLN PIVEERIW+RN FHYDNV KGLLTLFVVSTFEGWPGILYVSIDSN ED GPIH +RPIVA+FYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNP+QYKLWAFVTSP+FEYTIFAMIL+NTLSLAMKFY+QP+ YTAFLDVLNVIFTVFFTLEF+FKLGAFRFKNYFGDPWN+FDFIIVLGSLIDIAMAE+NPG NMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEG+NI+RNNNFQTF QALLVLFRSATGEAWQEIMLSC+K P V+CDE+SED G+P GCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILK+W+RTD+KLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETK L+E+ + +QS+TLQAGLRTLHEKGPELKRAISGNL+ IEA+D T + E + H+ N +FGNV+SSIK++ +LNVNS+QHAKISPTNS + QNSFRK+PDEDFP++A+ I M+PVKLANGGS DH RP
Sbjct: 783 RYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQAFGGVNSIGIVSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEAEKEEGAGPPEDGG--VEGVDILGANSHCGGDTELRLSLDQEYSLEDYDQY-------------AEDDEIVGDDDDEEDEGQ-------------------FELDDKN----TLRMDSESPFYKVVPIPQESAFYIFPPNNQFRILCHFICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCSDKSKMTAETCRGKFIVYKDGDLNQPIVEERIWERNDFHYDNVGKGLLTLFVVSTFEGWPGILYVSIDSNAEDVGPIHNYRPIVAVFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPIQYKLWAFVTSPVFEYTIFAMILINTLSLAMKFYQQPQTYTAFLDVLNVIFTVFFTLEFVFKLGAFRFKNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGSNINRNNNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKIWKRTDLKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKNLSENVESPTDQSLTLQAGLRTLHEKGPELKRAISGNLE--IEALDPTYGLEESFGDHSHESSQHKNN--YFGNVISSIKHKGMVPTTPTLNVNSLQHAKISPTNSFNSTGQQNSFRKIPDEDFPTNASGIPMKPVKLANGGSHDH-RP 1905 HSP 2 Score: 129.413 bits (324), Expect = 3.512e-30 Identity = 79/152 (51.97%), Postives = 94/152 (61.84%), Query Frame = 0
Query: 1193 LHPYYNANGDMVAPGGSGSKSVPSSPSDRKQS-----MPIV-GSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGSLEF---ADQPGWDED---DETVPLRAPPSRFMIPEHQPND--GPQAPDRRRR 1330
+H ANGD G S SVP+SP DR + +P+V GSAE+LVGRVLRDQGLGKYCDP+FVRAASREMQEAMDMTPEEFD AAH+LL A GSL + P D +E + PPS + + P P AP RR++
Sbjct: 2079 IHEEEVANGDSFVATGGVSXSVPNSPGDRSKGANASPVPVVVGSAESLVGRVLRDQGLGKYCDPEFVRAASREMQEAMDMTPEEFDAAAHQLLMAXXDGSLCLPTSSSPPSTRGDKWPEEELCSSPPPSPQGLNDLLPPQFVEPIAPVRRKK 2230 HSP 3 Score: 96.6709 bits (239), Expect = 3.735e-20 Identity = 94/344 (27.33%), Postives = 144/344 (41.86%), Query Frame = 0
Query: 242 YDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEET---CQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
YD ++ E +RR+ ++ P P + + + N R F ++L+ I+ + LA P +E N+IL +Y F IFT E +K+IAYGF HP A+ RS +N+LD +V + +IS + SS I VK LR RVLRPLR +V V ++ N L C K EE C+ EY +G N+ I ++D+ +LT+F T EGW +LY D+ G F I++ +++ AFF++N+ +G + F E E A + K
Sbjct: 125 YDTNKPIEKKPIRRIGAKPP-----PDRAPRSIYCWTIKNPIRKRFIQFVEWKPFEFLILLTILGNCVALAIYTPFPAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEGFDVKALRAFRVLRPLR----------LVSGVPREMEETDNPTL-----------------------CGGNYKCDEEAGYVCR----EYWEGP-NFGIT----------NFDHFGLSMLTVFQCITLEGWTDVLYWVQDA----MGSSWQF-----IYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQK 406 HSP 4 Score: 91.6633 bits (226), Expect = 1.261e-18 Identity = 123/614 (20.03%), Postives = 245/614 (39.90%), Query Frame = 0
Query: 73 NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAI----------AVDNLADADALEDEEKDEDD--------------PPEDGDDD------EEGKDGGED------GETKIPMDQYYP---DDYDQYDEVGRTYGQ--------------DGYA-VPSEDEYYQQDPS----QVVGQR------------VAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFF------LFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTD-RSKMTE--ETCQGQFIEYKDGDLNY-----------------------------------------PIVEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
NFD+F S++TVFQ +T E W V+Y A G +YF+ + G + ++N+ L + + D L +E+ EDD P DD+ + GK G+ T +P+ Q D++ + E ++ G GY ++ E + DPS ++G + +++ S L+NE DG G D + G E S++F L + + + R VC I I+++ + +++ +LA E + + + FF +FT E+ LK+ + GF G F S FN D VV S++ + ++ I + +LR +R+LR + + ++V+ ++ ++ +I +++++ L F+F+++G+Q+F GKF + RS ++C F ++D + + V+ + +N + ++D+ ++ LL++F + T E W ++Y I + G ++ + ++++ I I + ++N+F+ +
Sbjct: 328 NFDHFGLSMLTVFQCITLEGWTDVLYWVQDAMGSSWQF-----IYFVSXVVLGAFFVMNLILGVLSGEFSKEREKAQSRGDFQKLRQKEQMEDDLEGYMEWLTVAELIEPSPTDDNIGGLKVDVGKHKGDPVVMGLLKSTMLPLQQVLEGRGDEFSKEREKAKSRGDFQKHRERQQIEEDLRGYLDWITQAEDLENDPSLPPPNLLGAKNKDSSTNRSSKEISRDSFSARLTNEADGKGGALDVDGTG-------------------EPSSWFQKKKKALEKLNRRARRVCRKIVKSXAMFWIIIILVFLNTCVLATEH-YKQPIWLDDFQEFTNLFFVVLFTFEMFLKMYSLGF---QGYFV-SLFNRFDCFVVISSILELILTNENIMPPLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFTKSLESRSHFDTFPQSCLTVFQGFEDSERYWKRLQNLLGSLINSIXAIVSLLVLLFLFLGIFALLGTQVFGGKYVQSKSSGKNHYSRANFDSFSQSLLSVFQILTGEDWNVVMYDGIQA----FGGVNSIGIVSSLYFIILFICGNYILLNVFLAIAV 908 HSP 5 Score: 84.3445 bits (207), Expect = 2.111e-16 Identity = 50/186 (26.88%), Postives = 95/186 (51.08%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFR 792
V S + I ++ +NT LA + Y+QP F + N+ F V FT E K+ + F+ YF +N FD +V+ S++++ + N P G ++ R R++R K+ + L+ + S ++ + +++ L FI+ ++GMQ+FG + ++ ++F TFPQ+ L +F+
Sbjct: 598 VKSXAMFWIIIILVFLNTCVLATEHYKQPIWLDDFQEFTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTNENIMPPLG----LSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGG---KFTKSLESRSHFDTFPQSCLTVFQ 776 HSP 6 Score: 82.4185 bits (202), Expect = 8.005e-16 Identity = 42/79 (53.16%), Postives = 56/79 (70.89%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQ 86
LL + + RYW ++ NLV SL NSI SI SLL++LFLF+ IF+LLGMQ+FG +F + + R +FD F QS +TVFQ
Sbjct: 701 LLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIFGGKFTK---SLESRSHFDTFPQSCLTVFQ 776 HSP 7 Score: 63.1586 bits (152), Expect = 5.977e-10 Identity = 69/274 (25.18%), Postives = 115/274 (41.97%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAM--KFYRQPEGYT-AFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLS------KGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
KNP++ + FV FE+ I IL N ++LA+ F + T L+ + IF V FT E + K+ A+ F Y WN DF IV+ +I ++ + G + R FRV+R ++L+S + TL +K + G V + + EG N NF F ++L +F+ T E W +++ +DA G+ + + YF+S V+ +F ++NL + V+ F
Sbjct: 158 KNPIRKRFIQFVEWKPFEFLILLTILGNCVALAIYTPFPAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEG--FDVKALRAFRVLRPLRLVSGVPREMEETDNPTLCGGNYKCDEEA--------------GYVCREYW------EGPNFGI-TNFDHFGLSMLTVFQCITLEGWTDVLYWV------------QDA-----MGSSWQFIYFVSXVVLGAFFVMNLILGVLSGEF 391
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000004952 (supercontig:LSalAtl2s:LSalAtl2s260:1045447:1062229:1 gene:EMLSAG00000004952 transcript:EMLSAT00000004952 description:"maker-LSalAtl2s260-snap-gene-10.24") HSP 1 Score: 1001.12 bits (2587), Expect = 0.000e+0 Identity = 563/987 (57.04%), Postives = 667/987 (67.58%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSI-GILSSLYFIILFICGNYILLNVFLAIAVDNLADAD---------------ALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF--SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDI-AMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACK------------------------------IKMEGPIDQANDELKQIVLRIWKR 945
LL + + RYW +L NL+ SL NSI++I SL++LLFLF+ I+ALLG Q+FG +F +ILTGEDWN VMYDGI A+GG +++ G+ +SLYFI LFI GNYILLNVFLAIAVDNLA D A ++D PP G + ED I Y D DE +GY D Y D +Q+V + L E +++D N PIP S+ F+F N+FR++C+ CNH+YF N +LVCIMVSSA LAAEDPL+S S RN VIAYG + H GA+ R N+LDL+VV+VS+IS F S+GAISV+KILRVLRVLRPLRA+NRAKGLK V+ VIV+V TI NIVLVT+LLQF+FAVIGVQLFKGKF+ C+D SKMTEE CQGQ++++K D P V ER W N FHYDNVAKG+LTLFVVSTFEGWPGILY S+DS+ ED GP+ RP + IFY +YIIIIAFFMVNIFVGFVIVTFQ EGES + NCELDKNQRNCI++ LNAKP R Y+P +PVQY++WA+ S FEY +F IL+NT+SLAM FY QP+ Y+ FLD+LN IFT+FF LEF+ K+ AFR K YF DPWN FD IIV+GS IDI + + G ISINFFRLFR +RLVKLLSK E IRTLLWTF KSFQALP+V +LI L+FFIYGVVGMQVFGKIA E T+IH NNNFQ F AL+VLFRSATGEAWQ+IMLSC P V CDE+SE+AG+PAGCG+ AYPYFISFF+ SFLILNLFVAVIMDNFDYLTRDWSILG HHLDEFV LWSEYDPDAKG IKH+DVV LLRKI+PPLGFGKLCPHR ACK IKMEG ++ AN EL+ I+ +IW++
Sbjct: 446 LLRAFKVTRYWTSLKNLLKSLINSIEAIFSLIVLLFLFLGIYALLGSQLFGGKFXE----------------------RILTGEDWNAVMYDGILAYGGPQTVLGMFASLYFITLFIIGNYILLNVFLAIAVDNLAGDDDDEEXETPVVKDPEPAFAPAQQDPSRPPPPGTQIPHER---EDLLMMIDNHHLYNYDPGYEDEYNPYLNSNGY-----DHY--NDYNQMVNNNGPIKSLEEKLEEEKANEEAFLEDDD--------------NKKDPIPPFSSLFIFSPTNRFRVLCYNFCNHAYFANFILVCIMVSSASLAAEDPLHSKSPRNI--------------------VIAYGAVGHKGAYARYPANILDLVVVSVSIISIAFRDSAGAISVLKILRVLRVLRPLRAVNRAKGLKLVIXAVIVSVSTIQNIVLVTVLLQFLFAVIGVQLFKGKFYSCSDSSKMTEEECQGQYVDFKKSDWAQPTVLEREWSLNDFHYDNVAKGMLTLFVVSTFEGWPGILYTSLDSHAEDTGPLPISRPSMFIFYIVYIIIIAFFMVNIFVGFVIVTFQKEGESPFANCELDKNQRNCIDYTLNAKPARLYLPSDPVQYRIWAWAXSSKFEYVVFGAILLNTISLAMSFYNQPQIYSDFLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINEDTSIHSNNNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDNFDYLTRDWSILGAHHLDEFVNLWSEYDPDAKGCIKHMDVVQLLRKINPPLGFGKLCPHRNACKRLVAMNMPLRDDGTVEFQATLFAIIRTSLGIKMEGSLEXANSELRLIMKKIWRK 1366 HSP 2 Score: 95.5153 bits (236), Expect = 6.879e-20 Identity = 69/251 (27.49%), Postives = 119/251 (47.41%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF-MCTDRSKMT-----EETCQGQFIEYKDGDLNYPIVEERIWDRN--AFHYDNVAKGLLTLFVVSTFEGW 511
+ +LF N R+ C + F +L I+ + LA P ++ N L + F IFT E +K++AYG H A+ R+ +N +D +V + + + SS +I VK LR RVLRPLR ++ L+ V+ +++A+ + +I L+ L + ++++IG+Q+F+G C D C G + + + G + E I N ++DN +LT+F T EG+
Sbjct: 47 SIYLFSLKNPIRLKCITVVEWKAFEFFILATILATCVSLALHKPYPNGDTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGFMDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMFQGLMHKACFDNITGVLIDDDPRLCGGNY-QCETG-----VCREYIPGPNFGITNFDNFILSMLTVFQCITLEGF 291 HSP 3 Score: 78.5666 bits (192), Expect = 9.326e-15 Identity = 56/174 (32.18%), Postives = 91/174 (52.30%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAM-KFYRQPEGYT----AFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF 763
KNP++ K V FE+ I A IL +SLA+ K Y P G T FL+ + VIF V FT E + K+ A+ + Y + WN DF IV+ +DI ++ ++ G + R FRV+R ++L+S ++ +L + L +A+L+ + IY ++G+Q+F
Sbjct: 54 KNPIRLKCITVVEWKAFEFFILATILATCVSLALHKPY--PNGDTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGFMDILLSSLSIEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVIIIYSIIGLQMF 223 HSP 4 Score: 66.2402 bits (160), Expect = 6.682e-11 Identity = 51/193 (26.42%), Postives = 90/193 (46.63%), Query Frame = 0
Query: 669 FKNYFGDPWNAFDFIIVLGSLIDIAMA--EINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
F NYF +N FD +VL S++++ + I P + ++ R R++R K+ ++ LL + S +A+ + +L+ L IY ++G Q+FG F + +L TGE W +M G+L G P FA YFI+ F++ ++++LN+F+A+ +DN
Sbjct: 402 FWNYFISLFNRFDCFVVLSSILELVLTRTHIMPP---LGMSVLRCIRLLRAFKVTRYWTSLKNLLKSLINSIEAIFSLIVLLFLFLGIYALLGSQLFGG----------------KFXERIL------TGEDWNAVMYD-----GILA------YGGPQTVLGMFASLYFITLFIIGNYILLNVFLAIAVDNL 558
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000008334 (supercontig:LSalAtl2s:LSalAtl2s508:27226:174463:1 gene:EMLSAG00000008334 transcript:EMLSAT00000008334 description:"maker-LSalAtl2s508-snap-gene-1.26") HSP 1 Score: 271.552 bits (693), Expect = 1.350e-75 Identity = 155/371 (41.78%), Postives = 233/371 (62.80%), Query Frame = 0
Query: 267 PIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPK--NPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAM 632
PI S+FF+ N R+ H++ F ++ I++SS LA+EDP++ NS RNK+L Y DY FTTIFT+E LK+I GF+LHPGA+ R +N LD+ VV+ +LISF + +K L++LRVLRPL+ INR LK V CV++++K + NI++V +L Q +FA++GVQLF GKFF C+D S+ + CQG + Y+ + P + ER W+R +FHYDN + ++TLF + T IL S+ S E+ GP +R ++IFY ++ ++ FF VNIFV +IVTF GE+ ++ +DKNQ++CI+FA+ A+P+ Y+P+ + Y W +TS FE I +I+ NT+ L +
Sbjct: 655 PILPYSSFFILSSSNPIRVSIHWLXTKKVFDCFIMCVIVLSSIALASEDPVDENSIRNKLLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLDITVVSCALISFYHTLAGTPTGQKLKSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNVFNILIVYMLFQIIFAIVGVQLFNGKFFFCSDESRTNADECQGYYFHYEVPN-ETPKIVERTWERRSFHYDNCFRAIITLFAIQT------ILQDSMASTYENEGPKPWYRVEMSIFYIVFFVVFPFFFVNIFVALIIVTFNELGEAELQD-NMDKNQKSCIDFAIQARPLELYVPQRTDGFYYHAWRLITSSPFENFIMVLIICNTILLML 1017 HSP 2 Score: 119.783 bits (299), Expect = 2.217e-27 Identity = 73/128 (57.03%), Postives = 93/128 (72.66%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNL 141
+ +YW +L NLV +L N+I SI SLL LLFLF+ IFALLGMQ+FG FN+ P NF+ +L+TVFQ+LTGEDWN VMY I + GG K G++ SLYF++L +CG+Y LLNVFLAIA D+L
Sbjct: 452 VTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFS--TGTPTSNFNTITSALLTVFQVLTGEDWNQVMYTAIDSKGGRKGGGMVYSLYFVMLTLCGDYTLLNVFLAIACDSL 577 HSP 3 Score: 68.5514 bits (166), Expect = 1.172e-11 Identity = 83/329 (25.23%), Postives = 146/329 (44.38%), Query Frame = 0
Query: 538 IFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNC---ELDKNQRNCIEFALNA-KPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVL-NVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 860
IF+ II+ +FFM+N+F+G + F E E +L + Q+ +E LN + ++I +N E T F F+R + F+ + IF F LE + +L A + YF +N FD +++GS++++ G ++ R R++R+ K+ +R L+ + ++ + L+ L FI+ ++GMQ+F G GT +NF T ALL +F+ TGE W ++M + D K G G G ++ YF+ + + +LN+F+A+ D+ D
Sbjct: 286 IFFIPLIIVGSFFMLNLFLGVLSGEFAKEKERVESRVGFMQLKEQQK--LEKELNGVQKTHKFIKEN---------------EQTSFK----KEFDDDSNFWRSERQFRFFIRLYAEFIFLGCFILEILIRLYALGPRTYFSTTFNVFDCFVIVGSIVEVIWVHFKDKGGSFGLSALRALRLLRVFKVTKYWSSLRNLVIALMNAISSIVSLLFLLFLFIFIFALLGMQIFGGSFNFSTGT---PTSNFNTITSALLTVFQVLTGEDWNQVMYTA-------IDSK----GGRKGGGMVYSL-YFVMLTLCGDYTLLNVFLAIACDSLD 578 HSP 4 Score: 52.373 bits (124), Expect = 9.659e-7 Identity = 46/189 (24.34%), Postives = 78/189 (41.27%), Query Frame = 0
Query: 252 RMRRMISESPVNPTMPIPEA--SAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAED--PLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLI------SFVFSSGAISVV--------------------------KILRVLRVLRPLRAIN 404
R ++ E + + P S+ F N R + F I++ I+ + ++A + P S+ + L + F IFT+E+C K++A GFILH G++ R+ +N++D +VV I SF+ S V+ LR RVLRPL+ ++
Sbjct: 34 RRTHLVMEKSLEMKLHHPNKLPSSLFFLSGKNPIRRAVIWFVRWIVFEWIIIFTIIANCIVMALDQHLPHKDKSKLSVQLEELEPVFMGIFTMEMCFKIVADGFILHHGSYLRNPWNIMDFIVVISGSIYVHPNKSFIHISHYNDVIYYFYSTSFLPYLIPKSDKKENTGPDLSTLRTFRVLRPLKLVS 222
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000005510 (supercontig:LSalAtl2s:LSalAtl2s297:203090:236595:1 gene:EMLSAG00000005510 transcript:EMLSAT00000005510 description:"maker-LSalAtl2s297-augustus-gene-2.17") HSP 1 Score: 261.922 bits (668), Expect = 8.552e-72 Identity = 145/338 (42.90%), Postives = 207/338 (61.24%), Query Frame = 0
Query: 245 DEEDEGDR-MRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAIS-VVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQ 580
DEE+ G+ + M E V P +P S+ F+ N FR+ H+ +F +++ I++SSA LAAEDP+ + RN +L YFDY FT IF +E LK++ G HPG++ R +N+LD LVV+ +LISF F + +K +++LRVLRPL+ +NR LK V CV+ ++K + NI++V +L +FA++GVQLF GKFF C+D SK E CQG++ + + P VE R+WD AF+YDN ++TLF V T EGW +L S+ S ED GP F +AIFY +Y ++ FF VNIFV VIVTF GE+ + ++DKNQ
Sbjct: 285 DEEEGGEPPLPDMDDEDQVRPILPY---SSLFILTSTNPFRVGLHWFVTKPFFDGFIMIVILLSSASLAAEDPVEEHHPRNLLLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALISFYFQGTPMGQKLKSVKLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNSMSSTFEDEGPFPWFSTEMAIFYIVYFVVFPFFFVNIFVALVIVTFNELGEAELTD-DIDKNQ 618 HSP 2 Score: 149.828 bits (377), Expect = 1.389e-36 Identity = 193/845 (22.84%), Postives = 352/845 (41.66%), Query Frame = 0
Query: 290 ICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF-----SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNE--------------------------------GESAYKNCELDKNQRNCIEFAL-------NAKPVRRY------IPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMK-FYRQPEGYTAFLDVLNVIFTVFFTLEFIFK---LGAFRFK-NYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF----------------------------GKIAPQEGTNIH--RNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFV--RLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSI--KTEGNIDEANEELRHQILKVW--RRTDIKLLDQICPGAGL-EDEVTVGKFYATFLIQDYFR 1042
+C ++ +V++ + +++ +A E N L Y ++ F F E+C+++ A G P + S FN D +V+ S+ ++ +G+ + LR LR+LR + L+++V ++ A+ +I +++ + L F+FA++G+QLF G F N+P + +++ + LLT+F V T E W ++Y +I S G H I +I++ + ++ + ++N+F+ + G EL ++ E L +P+ Y NP + L FVT P F+ I +IL+++ SLA + + L + +FT F +E + K LGA +Y D WN D ++V +LI G + S+ +L RV+R +K++++ ++ + S + + + I+ L I+ +VG+Q+F G+ +P + + N+ A++ LF T E W +++ + ED G T+ A ++I +FVV F +N+FVA+++ F+ L G L + + + + G+I + D+ LL++I PPLGFG CP +A K RLV MNMP++++G V+FN TLFALVR +L I ++ +D+A++ELR I + W + D KL + P + +++TVGK + LI + ++
Sbjct: 1 MCKQQWWFWLVIILVFLNTCTVAVEH-YNQPQWLTIFLYYAEFLFLGAFVFEMCIRLYALG----PFIYFSSAFNRFDCVVITGSIFEVLWVNLQPRAGSFGLSG-LRALRLLRVFKVTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSF--------------------------NFPD------ETPTSNFNTIINALLTVFQVLTEEDWNNVMYXAIVS----QGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSLDQAAELTAAEEAEKERQEEEQERQRLREQQELKAIGADGQLGDELRGDEEEGGEPPLPDMDDEDQVRPILPYSSLFILTSTNPFRVGLHWFVTKPFFDGFIMIVILLSSASLAAEDPVEEHHPRNLLLQYFDYVFTAIFAIECLLKILDLGACAHPGSYLRDIWNVLDSLVVSCALISFYFQGTPMGQKLKSV---KLLRVLRPLKMVNRVPALKAVFDCVVTSLKNVFNILIVYMLFLLIFAMVGVQLFNGKFFYCSDASKGNAEECQGEYFVFGEHGETSPTVERRVWDIKAFNYDNCGAAIITLFAVQTSEGWVDVLQNS-------MSSTFEDEGPFPWFSTEMAI-FYIVYFVVFPFFFVNIFVALVIVTFNEL-------GEAELTDDIDKNQLRQQESWNGGKIHYTDMFALLKQIDPPLGFGSKCPDLLAYK--------------------------------RLVRMNMPVDNEGKVHFNTTLFALVRVNLQIFMRSTDEMDQADQELRTTIGRSWPFTKRDGKLDLLVPPSSETGPNKLTVGKIFGGMLILENWK 753 HSP 3 Score: 112.849 bits (281), Expect = 2.829e-25 Identity = 70/128 (54.69%), Postives = 91/128 (71.09%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNL 141
+ +YW L NLV +L N++ SI SLL LLFLF+ IFALLGMQ+FG FN+ + P NF+ +L+TVFQ+LT EDWN VMY I + GG + GI+ S+YF++L + GNY LLNVFLAIA D+L
Sbjct: 112 VTKYWSPLRNLVIALMNALSSIMSLLFLLFLFIFIFALLGMQLFGGSFNFP--DETPTSNFNTIINALLTVFQVLTEEDWNNVMYXAIVSQGGRHNGGIIYSIYFVMLTLLGNYCLLNVFLAIACDSL 237
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000010768 (supercontig:LSalAtl2s:LSalAtl2s712:648445:661651:1 gene:EMLSAG00000010768 transcript:EMLSAT00000010768 description:"maker-LSalAtl2s712-snap-gene-6.14") HSP 1 Score: 250.366 bits (638), Expect = 1.954e-67 Identity = 200/660 (30.30%), Postives = 328/660 (49.70%), Query Frame = 0
Query: 284 RIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP-LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPG-AFCRSGFNLLDLLVVAVSLISF---VFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTF---QNEGESAYKN--CELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVM---RLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLIL-NLFVAVIMDNFDYLTRD-WSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPI 928
R+ + + YF +VL I++SS ++ ED ++ +L Y D T +F +E LK+ A G +++ G A+C LD ++VAVSLI+F + G I + K +R LR LRPLRA+ + +G+K VV ++ A+ +I N++LV L+ +FA+IG+ F GKF+ C D T E + I K LN +W ++DNV L LF V+TF+GW I+ +IDS E+++ P + F+ +I+ +FF +N+ VG +I F +N+G S+ E K ++ A KP++ +P+ +K A V S I+ N + G+T LD LN+ F V FT E + K+ A R +YF +PWN FDF++V+ SL + ++++ +S R+ RV R+++L+ +GIRTLL++ +F AL + +L+ L+ FI+ V GM +F + + G + T W E + + D CD+ S + GD CG A F+ +++ SFLI+ N+++AVI++N+ D + D F +W E+DPD + + + L + PPL K ++ I M+ PI
Sbjct: 1175 RLQVSRLIENKYFEGVVLTLILLSSFVMTLEDIWFDTRPLLVDMLYYLDRILTVVFFLETLLKLFAMGCVMYFGNAWC-----WLDFVIVAVSLINFGASLVGLGNIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKFYKCLDEE--TGEKFSHEIIPNKTVCLNE---TGAVWTNARVNFDNVFIAYLALFQVATFKGWMTIMDDAIDSVEKNNQPHREININMYFFFVFFIVFGSFFTLNLLVGVIIDKFNEQKNKGGSSLDAFMTEDQKKYIAAMKKASTKKPLKA-LPRP--NWKPQAIVFS----------IITN----------KNAGWTFALDNLNLSFIVIFTAEMLLKMFALR-HHYFAEPWNLFDFVVVMLSLAGLFLSDL-IEKYFVSPTLLRVVRVAKVGRVLRLIKGAKGIRTLLFSLVMAFPALVNICLLLFLVMFIFAVFGMSLFKNVKIRSGFD------------------DMCTSAGWAEALDAITDDRE--CDKPSSETGDAGNCGNYMAGVAFMIIYLILSFLIIVNMYIAVILENYSQANEDVQEGITDEDYDLFYEIWQEFDPDGTQYMPYKSLSEFLDXLEPPLQIAKPNKFKI---IHMDIPI 1776 HSP 2 Score: 82.8037 bits (203), Expect = 5.132e-16 Identity = 67/223 (30.04%), Postives = 111/223 (49.78%), Query Frame = 0
Query: 605 KLWAFVT-SPIFEYTIFAMILVNTLSLAMKFYR----QPEGYTAFLDVL----NVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGK-----IAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVL 813
++ AF+ P E I I VN + +A+ Y G + FL + N FT F +E KL A + +F + WN FDF+IV+ SL+++ +AE G +M+ R FR++R+ KL + + +L +F AL + ++ +I FI+ V+GMQ+FGK + + ++ R NF F + +++FR GE W E + K G +
Sbjct: 739 QILAFIVFDPFTELFITICIAVNVIFMALDRYDIEYDANGGMSPFLSSVLTQGNYFFTTIFAVESFIKLVAMSPRYFFSEGWNCFDFLIVVLSLVEL-LAEGVSGLSML-----RSFRLLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGKDYYDGVCEKWDCDMPR-WNFTDFLHSFMIVFRVLCGE-WIESIWVVKGIVGTV 953 HSP 3 Score: 70.8626 bits (172), Expect = 2.714e-12 Identity = 39/127 (30.71%), Postives = 69/127 (54.33%), Query Frame = 0
Query: 324 NKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF 450
+ +L +YFFTTIF VE +K++A + P F G+N D L+V +SL+ + + +S + +LR R+LR + K L ++ + + N+ V ++ F+FAV+G+QLF ++
Sbjct: 785 SSVLTQGNYFFTTIFAVESFIKLVA----MSPRYFFSEGWNCFDFLIVVLSLVELL--AEGVSGLSMLRSFRLLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGKDYY 905 HSP 4 Score: 61.2326 bits (147), Expect = 1.826e-9 Identity = 30/89 (33.71%), Postives = 56/89 (62.92%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQK-----PRGNFDNFFQSLITVFQILTGE 91
LL + + WK+L++++ + N+ ++++L +L + + IFA++GMQ+FG + YD + +K PR NF +F S + VF++L GE
Sbjct: 851 LLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLFGKDY-YDGVCEKWDCDMPRWNFTDFLHSFMIVFRVLCGE 938
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000009714 (supercontig:LSalAtl2s:LSalAtl2s627:140867:158129:1 gene:EMLSAG00000009714 transcript:EMLSAT00000009714 description:"maker-LSalAtl2s627-snap-gene-1.23") HSP 1 Score: 239.58 bits (610), Expect = 3.699e-64 Identity = 235/812 (28.94%), Postives = 386/812 (47.54%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQK-----PRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDP-------------------PEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYA-----VPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNP----TMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP-LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFS-SGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFI-YIIIIAFFMVNIFVGFVIVTF---QNEGESAYKNCELDKNQRNCI--------EFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFG--KIAP 768
LL + + WK+L++++ + N++ ++++L ++L + + IFA++GMQ+FG+ F + +K PR NF +F S + VF++L GE W M+ + G YF+ + N ++LN+FLA+ + + +D +EE + D E+ D K + IP + + G ++ +GY VPSE + Y Q S++F D+ EE D + + E P +P+ + + F ++ Q R++ + + YF +VL I++SS ++ ED + L Y D T +F +E LK+ A G P + + + LD ++VA SLI+F+ S GA+++ K +R LR LRPLRA+++ +G+K VV +I A+ +I N++LV ++ +FA+IGV LF G F C D + + I K LN + W + +DNV L L V+T++GW I+ +IDS + P I FYF+ +II+ +FF +N+ VG +I F +N+G S+ + + ++Q+ I + L A P + P Q ++ VT+ F+ I I +N L++ M Y+Q EG+T LD LNV F + FTLE + K+ A R + YF +PWN FDF IVL SL GG L + +GIRTLL++ + AL + +L+ L+ FI+ V GM +F KI P
Sbjct: 727 LLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGS-FYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGE-WIESMWACMYVAGPT------CVPYFLATVLVANLVILNLFLALLLSSFSDMGGSGEEEDGKPDKMSIAVNRFKRLFSFTKRKIKEELFDRIXRKIAHSKSKPLIP----------EANGNGFSHKTNGYIGGHNNVPSETQTYIPIDQQ---------------SDKFAPQTKDH-EEIYYDPLETLEVEDCCPPFVYRIIPLGDQHSPF-WEAWTQQRLMAMRLIENEYFEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMG----PVMYFTNAWCWLDFVIVAXSLINFIASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCID--SIXGHKFSHEIIPNKTVCLNQTNAQ---WINSKVTFDNVIMAYLALLQVATWKGWITIMDDAIDSVAINKQPYRE-ASIGMYFYFVAFIILGSFFTLNLLVGVIIDKFNEQKNKGGSSL-DAFMTEDQKKYIAAMKKASKKKPLKALPRPYWKP----QAIVFGIVTNKKFDMIIMGFIGLNMLTMMMDHYQQTEGWTFVLDNLNVFFIMIFTLEMLLKIFALR-QYYFSEPWNLFDFFIVLLSL----------GG------------------LFLRAKGIRTLLFSLVMALPALGNICLLLFLVMFIFAVFGMSLFKNVKIIP 1459 HSP 2 Score: 85.1149 bits (209), Expect = 9.584e-17 Identity = 50/170 (29.41%), Postives = 84/170 (49.41%), Query Frame = 0
Query: 649 NVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNN----NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLC 814
N FT F E KL A + +F D WN FDF+IV+ SL+++ +N ++ R FR++R+ KL + + ++ + AL + +++ +I FI+ V+GMQ+FG ++ + NF F + +++FR GE W E M +C G C
Sbjct: 668 NYFFTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILSLVELLAXGVN------GLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGSFYIEKACEKWKCELPRWNFTDFLHSFMIVFRVLCGE-WIESMWACMYVAGPTC 830 HSP 3 Score: 72.4034 bits (176), Expect = 7.242e-13 Identity = 54/176 (30.68%), Postives = 90/176 (51.14%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSS-AMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKG 447
A F + R + + H F I++ I+V+ M+ + S SE FT+I+T E +K++A GFILH + R +N LD V+ +S I+ G+ S LR RV R L+++ GLK +V +I +VK + ++ ++TL +FA++G+QL+ G
Sbjct: 146 ALFFLTPFHPIRRIAIRVLTHPLFNFIIICTILVNCIVMMLPDTEYXSASE---------IIFTSIYTYESAVKLLARGFILHNFTYLRDPWNWLDFAVIGMSYITIAIDLGSFSS---LRTFRVFRALKSVAXVPGLKTIVGAIIYSVKNLFDVTILTLFGLALFALLGLQLYMG 309 HSP 4 Score: 72.4034 bits (176), Expect = 8.220e-13 Identity = 67/273 (24.54%), Postives = 120/273 (43.96%), Query Frame = 0
Query: 615 FEYTIFAMILVNTLSLAMK--FYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-----------------GKIAPQEGTNIHRNN--------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 860
FE + +IL+++ + ++ ++ L L+ I TV F LE KL A YF + W DF+IV SLI+ +A + N+ R R +R ++ +SK EG++ ++ + ++ V ++ + + I+ ++G+ +F +I P + +++ N F A L L + AT + W IM D + ++ A G Y YF++F ++ SF LNL V VI+D F+
Sbjct: 1013 FEGVVLGLILLSSFVMTLEDIWFETRPVLIDCLYYLDRILTVVFFLETTLKLFAMGPVMYFTNAWCWLDFVIVAXSLINF-IASLVGAVNIPIFKTMRTLRALRPLRAMSKMEGMKVVVNALIGALPSIFNVLLVCIVFWLIFAIIGVNLFMGTFHKCIDSIXGHKFSHEIIPNKTVCLNQTNAQWINSKVTFDNVIMAYLALLQVATWKGWITIM-----DDAIDSVAINKQPYREASIGM---YFYFVAFIILGSFFTLNLLVGVIIDKFN 1276 HSP 5 Score: 69.3218 bits (168), Expect = 6.135e-12 Identity = 44/149 (29.53%), Postives = 79/149 (53.02%), Query Frame = 0
Query: 322 ERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEY 470
E N +L +YFFTTIF E +K++A + P F G+N D L+V +SL+ + + ++ + +LR R+LR + K L ++ + + + N+ +V ++ F+FAV+G+QLF G F++ K E + F ++
Sbjct: 659 EMNNLLKNGNYFFTTIFAFESFMKLMA----MSPRYFFADGWNCFDFLIVILSLVELL--AXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLF-GSFYIEKACEKWKCELPRWNFTDF 800 HSP 6 Score: 55.0694 bits (131), Expect = 1.402e-7 Identity = 54/189 (28.57%), Postives = 88/189 (46.56%), Query Frame = 0
Query: 574 CELD---KNQRNCIEFALN-------AKPVRRYI-PKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFK----NYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAIL 747
C++D +QR + A N A+P ++ P +P++ +T P+F + I ILVN + + + P+ T + +IFT +T E KL A F Y DPWN DF ++ S I IA+ ++ S + R FRV R +K ++ G++T++ S + L V IL
Sbjct: 117 CDIDPFYADQRTFMVIAKNGSITRFSAEPALFFLTPFHPIRRIAIRVLTHPLFNFIIICTILVNCIVMML-----PD--TEYXSASEIIFTSIYTYESAVKLLARGFILHNFTYLRDPWNWLDFAVIGMSYITIAI-------DLGSFSSLRTFRVFRALKSVAXVPGLKTIVGAIIYSVKNLFDVTIL 291
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000007884 (supercontig:LSalAtl2s:LSalAtl2s464:432083:573266:-1 gene:EMLSAG00000007884 transcript:EMLSAT00000007884 description:"maker-LSalAtl2s464-augustus-gene-6.21") HSP 1 Score: 224.942 bits (572), Expect = 1.407e-59 Identity = 162/539 (30.06%), Postives = 256/539 (47.50%), Query Frame = 0
Query: 216 PSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIP-------------------------EASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP-LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQN---EGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVK 719
PS V+ + K PS N +G + + M+ ES N T IP +A ++F +++ R+ C I + +F IVL+ I + LA E P + S K+LN + FT IFT+E+ +K I+ G I A+ ++G+N++D +V VS + V SS ++++ R++R LRPLR INRA GLK VVQ ++ ++K IGNIVL+ +F ++GVQLFKG F+ C E ++ K L R W + +++DN+ L++LFV+S+ +GW I+Y +D+ D P F I++ +++++ FF++N+FVG V+ F E E + K + E YI + + + VTS F I A+I +N +++AM+FY P L V N FT F LE K+ A Y D WN D +IVL S++ I + EI I+ R+ RV+R+ +
Sbjct: 1200 PSSVITENDLK----PSTKNGANG---------RSNSIHMMLEESSNNSTSEIPRNPIYRIRYWLLMGLNKWRRFLYRRDAYTLYVFHPNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYC--------EGPHANIVKNKTQCLELSDKGNR-WINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRCRAEQEKEERARRAAKRAKKIEERRRRMMKAPYYIHYSHYRLSVHKVVTSKYFXLAISAVIGLNVITMAMEFYMMPVVLAYALKVCNYFFTAVFILEAGMKISALGIGRYLSDKWNQLDVVIVLLSVVGIVLEEIKSDMMPINPTIMRVMRVLRIAR 1716 HSP 2 Score: 106.301 bits (264), Expect = 3.540e-23 Identity = 76/282 (26.95%), Postives = 135/282 (47.87%), Query Frame = 0
Query: 586 FALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDI---AMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFG-----KIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
F +K +RR+ Q L V IF+ I IL+NT S+ ++F++QPE T ++V N++F+ F +E I K+ A+ Y D +N FD +IV+ S I+I + I GN ++ R FR++R++KL+ +R L K+ + L+ L FI+ ++GM +FG K+ Q + NF + A + +F+ T E W ++ S + + +A YF++ ++++ NL VA++++ F
Sbjct: 776 FLFCSKGIRRF------QAALERIVDHNIFQRGILFCILINTFSMGIEFHKQPELLTKIVEVSNLVFSAIFAVEMILKVSAYGIFRYISDGFNVFDGLIVILSSIEIYNGLIHGIEMDGNS-GLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKFCDKVGNQRKCD---RKNFDSLLWATVTVFQILTQEDWNIVLFSGMQRT------------------SHWAALYFVTLMTFGNYVLFNLLVAILVEGF 1029 HSP 3 Score: 94.3597 bits (233), Expect = 1.686e-19 Identity = 61/192 (31.77%), Postives = 96/192 (50.00%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKP--RGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPM----DQYYPDDYDQYDE 193
LL + R+ L + + ++ ++A LL LF+ IF++LGM +FG +F Q+ R NFD+ + +TVFQILT EDWN+V++ G+Q ++LYF+ L GNY+L N+ +AI V+ ++ D EK D + D GK + P+ D ++ D D DE
Sbjct: 900 LLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKFCDKVGNQRKCDRKNFDSLLWATVTVFQILTQEDWNIVLFSGMQRTSH------WAALYFVTLMTFGNYVLFNLLVAILVEGFSNQD-----EKRSSDRNSEYSKDPVGKLDDFSSQINSPIRYKNDMFHGDSLDYPDE 1080 HSP 4 Score: 92.8189 bits (229), Expect = 5.186e-19 Identity = 74/291 (25.43%), Postives = 134/291 (46.05%), Query Frame = 0
Query: 598 PKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQP--EGYTAFLDVLNVIFTVFFTLEFIFKLGA----FRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMI--SINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEG--TNIHRNN------------------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
P + ++ K + F++ + I N ++LAM+ P L+VLN IFT FT+E K + + + YF + WN D +V+ S +D + + I + FRL R +R ++++++ G++ ++ T S + + + ++ F I+G++G+Q+F G EG NI +N NF AL+ LF ++ + W IM G+ D++ P ++ YFISF ++ F +LN+FV V+++NF
Sbjct: 1275 PNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINRAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGL--DQQ------PKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENF 1557 HSP 5 Score: 73.559 bits (179), Expect = 3.338e-13 Identity = 78/310 (25.16%), Postives = 141/310 (45.48%), Query Frame = 0
Query: 282 QFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSER-NKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKI-----LRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVL---VTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIA-FFMVNIFVGFVIVTFQNEGE--SAYKNCELDKN 579
+F+ I +H+ F +L CI++++ + E + E KI+ + F+ IF VE+ LKV AYG + GFN+ D L+V +S I +G I +++ L VLR R LR + + L ++ + ++V +KT+ N+ + + +L F+F+++G+ LF KF C K G+ +R DR F D++ +T+F + T E W +L+ + A YF+ ++ + + N+ V ++ F N+ E S+ +N E K+
Sbjct: 785 RFQAALERIVDHNIFQRGILFCILINTFSMGIE--FHKQPELLTKIVEVSNLVFSAIFAVEMILKVSAYGIF----RYISDGFNVFDGLIVILSSIEIY--NGLIHGIEMDGNSGLSVLRTFRLLRILKLVRFLPNLRRQLVVMLKTMDNVAVFFALLMLFIFIFSILGMNLFGCKF-------------CD------KVGN-------QRKCDRKNF--DSLLWATVTVFQILTQEDWNIVLFSGMQRTSH-----------WAALYFVTLMTFGNYVLFNLLVAILVEGFSNQDEKRSSDRNSEYSKD 1047 HSP 6 Score: 65.4698 bits (158), Expect = 9.287e-11 Identity = 55/187 (29.41%), Postives = 91/187 (48.66%), Query Frame = 0
Query: 264 PTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL---NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKG 447
P E S FL N R C I + +F + + I+ + L P + + R +IL D FTVE+ +K+IA G + G + +N LD +V + + +++ LR +RVLRPLRAINR ++ +V ++ + +GN++L+ + F+F +IGVQL+ G
Sbjct: 27 PASEFAEYSFRFL-NLSNPVRKFCIRILVNPWFERLTMTVILFNCVTLGMYHPCEDTDCKTRRCQILKNCDDVIYIYFTVEMFIKMIAMG-VFGKGCYLSETWNRLDCFIVLAGALEYCLKVEKLNL-STLRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 210 HSP 7 Score: 52.7582 bits (125), Expect = 7.716e-7 Identity = 38/152 (25.00%), Postives = 70/152 (46.05%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNY-------------DPLAQKPRG--------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFG----GVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADA 146
L +V +L +S++ I +++L+ F IF +LG+Q+F F Y L +G NFDN +L+++F + + + W +MY G+ A G ++ +YFI + + +LN+F+ + V+N A
Sbjct: 1411 GLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRCRA 1562
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000003972 (supercontig:LSalAtl2s:LSalAtl2s2133:21116:38912:-1 gene:EMLSAG00000003972 transcript:EMLSAT00000003972 description:"maker-LSalAtl2s2133-snap-gene-0.4") HSP 1 Score: 198.749 bits (504), Expect = 1.661e-55 Identity = 106/225 (47.11%), Postives = 141/225 (62.67%), Query Frame = 0
Query: 366 LDLLVVAVSLISFVF----SSGA--ISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCI 584
+D+LVV+ ++ SF SSG + +K LRVLRVLRPL+ I R LK V CVI ++K + NI++V +L F+FAVI VQLF GKFF C+D SK E +G++ + + P V R+W R FHYDNVA +LTLF V T EGWP IL S+ + ED PI FR ++IFY +Y I+ FF VNIFV +I+TFQ +GES + E+DK C+
Sbjct: 1 MDMLVVSCAITSFAMDMLGSSGGENLGTMKSLRVLRVLRPLKTIKRVPKLKAVFDCVINSLKNVFNILVVYILFHFIFAVIAVQLFNGKFFYCSDASKQDAE--EGEYFLFSTVNDFPPQVLPRVWKRQRFHYDNVATAILTLFAVQTTEGWPAILQQSMAATFEDQAPIPFFRVGMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGESELEEAEIDKKSVECV 223 HSP 2 Score: 180.259 bits (456), Expect = 4.124e-49 Identity = 101/239 (42.26%), Postives = 138/239 (57.74%), Query Frame = 0
Query: 717 LVKLLSKGEGIRTL----LWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAG--------CGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIW 943
++KL+S G G ++ F F+ALP+V +LI ++FFIY ++GMQ+FG I T +R+ NFQTF ++VLFR ATGEAW IML+C + G DE++ +G CG+ +Y YF+SF +CSFL+LNLFVAVIMDNFDYLTRD SILG HHLDE GRI + ++ +L+ + PPLGF C ++LK+ + RIW
Sbjct: 223 VLKLISYGPGSYFKDAWNIFDFVTXFKALPYVILLIAMLFFIYTIIGMQIFGNIEFNPETEYNRHVNFQTFAAGIMVLFRCATGEAWPSIMLAC--EAGKRXDERNHKYNLTSGKILDPEKTCGSSMSYVYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDE-------------GRIYYTEMYDMLKNMDPPLGFVSKC------------------EQLKKWIRRIW 428
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000006480 (supercontig:LSalAtl2s:LSalAtl2s354:213665:368876:-1 gene:EMLSAG00000006480 transcript:EMLSAT00000006480 description:"maker-LSalAtl2s354-snap-gene-2.25") HSP 1 Score: 193.356 bits (490), Expect = 8.474e-50 Identity = 173/640 (27.03%), Postives = 286/640 (44.69%), Query Frame = 0
Query: 276 LFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP-LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGA------FCRSGFNLLDLLV-------------------------------VAVSLISFVFS---SGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERI-WDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSN--EEDHG-----------------------PIHGFRPI---------VAIF------------------------------YFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCE--LDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVM---RLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIM 803
+Q RI + + YF V+V I++SS LA ED L S +L Y D FT IF +E+C+K +A GF+ + F F++L+L+ + VSLI+F S +G I K +R LR LRPLRA++R +G++ VV ++ A+ +I N++LV L+ +FA++GVQ+F GK++ C D +T E + + D+ + E W + +D+V ++LF V+TF+GW I+ +IDS E+ G +H F + VAIF YF++ II +FF +N+F+G +I F + + + E + +Q+ ++ K + P P Q ++ VT+ F+ I I +N L++ + Y Q E ++ LD LN+ F V FT E + K+ A R + F IVL LI+ +S R+ RV R+++L+ GIRTLL+ S AL + +L+ L+ FI+ + GM F + + G + NF+TF +++++LF+ +T W ++
Sbjct: 1182 FWQGWGNLRIKTFRLIENKYFETAVIVMILISSLALALEDVYLPSRPVLQDVLYYMDRIFTVIFFLEMCVKWLALGFVKYFTNAWXWLDFVXVMFSILNLVASFFGVGNIPIFKTIRTLRGLRPLRALSRIGGMKVSLINFTASLLGAGGIQAFKTMRTLRALRPLRAMSRMQGMRXVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQMFAGKYYKCVD--IVTGEKISAEIVP----DVVTCLAENNTEWVNSRITFDHVGHAYISLFQVATFKGWLPIMNDAIDSRDCEDAEGNRLNASYTPDKETCLLENQYWVNSVHNFDNVGSAYLSLFQVAIFKGWTGVLYDALDSSGIGQQPIREVNLYMYWYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKIRGSLEMFMTDDQK---KYYAAMKKMGNKKPWKP-QAVVFGIVTNKKFDMIIMIFIGLNMLTMTLDHYGQSEMWSFALDNLNIGFIVIFTTECVLKIFALRL----------YYFRIVLSDLIE---------KYFVSPTLLRVVRVAKIGRVLRLVKGARGIRTLLFALAMSMPALFNICLLLTLVMFIFAIFGMSFFMNVKKRGG--LDDVYNFETFGKSMILLFQMSTSAGWDGVL 1790 HSP 2 Score: 85.5001 bits (210), Expect = 9.310e-17 Identity = 66/209 (31.58%), Postives = 96/209 (45.93%), Query Frame = 0
Query: 613 PIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHR-------NNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLC 814
P + I ILVNT + + + L N FT F +E KL A K YF + WN FDFIIV SLI++ + N+ + R FR++R+ KL + L+ K+ AL + +++ +I FI+ V+GMQ+FGK NI R NF F + +++FR GE W E M C +C
Sbjct: 712 PFVDLFITLCILVNTFFMGLDHHDMDPDIHKTLQNGNYFFTATFAIESCXKLMAMSPKYYFLEGWNIFDFIIVSLSLIELGLV------NVSGLTVLRTFRLLRVFKLAKSWPTLNLLISIMGKTVGALGNLTLVLCIIIFIFAVMGMQLFGK---SYVDNIDRFPNKSLPRWNFVDFMHSFMIVFRVLCGE-WIESMWDCMWIASKVC 910 HSP 3 Score: 85.1149 bits (209), Expect = 1.169e-16 Identity = 50/164 (30.49%), Postives = 93/164 (56.71%), Query Frame = 0
Query: 284 RIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKG 447
R V +I H +F ++ I+++ M+ + +++ER + + FT I+T E KV+ GFIL P ++ R +N LD +V+ ++ I+ G ++V LR RVLR L+ + GLK +V VI +VK + +++++T+ +FA++G+Q++ G
Sbjct: 110 RRVAIHILVHPFFSVFIITTILLNCLMM-----IKTSNER---IESSEVIFTAIYTFESATKVMGRGFILCPFSYLRDAWNWLDFIVITLAYITMGIDLGNLAV---LRTFRVLRALKTVAIIPGLKTIVGAVIESVKNLRDVIILTVFSLSVFALLGLQIYMG 262 HSP 4 Score: 74.3294 bits (181), Expect = 2.088e-13 Identity = 45/156 (28.85%), Postives = 84/156 (53.85%), Query Frame = 0
Query: 300 VLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDR 455
+ +CI+V++ + D + + + +K L +YFFT F +E C K++A + P + G+N+ D ++V++SLI + +S + +LR R+LR + L ++ + V +GN+ LV ++ F+FAV+G+QLF + DR
Sbjct: 718 ITLCILVNTFFMGL-DHHDMDPDIHKTLQNGNYFFTATFAIESCXKLMA----MSPKYYFLEGWNIFDFIIVSLSLIELGLVN--VSGLTVLRTFRLLRVFKLAKSWPTLNLLISIMGKTVGALGNLTLVLCIIIFIFAVMGMQLFGKSYVDNIDR 866 HSP 5 Score: 57.3806 bits (137), Expect = 3.415e-8 Identity = 33/96 (34.38%), Postives = 56/96 (58.33%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARF--NYDPLAQK--PRGNFDNFFQSLITVFQILTGEDWNVVMYD 99
LL + + W L+ L++ + ++ ++ +L L+L + + IFA++GMQ+FG + N D K PR NF +F S + VF++L GE W M+D
Sbjct: 807 LLRVFKLAKSWPTLNLLISIMGKTVGALGNLTLVLCIIIFIFAVMGMQLFGKSYVDNIDRFPNKSLPRWNFVDFMHSFMIVFRVLCGE-WIESMWD 901 HSP 6 Score: 55.8398 bits (133), Expect = 9.553e-8 Identity = 46/177 (25.99%), Postives = 80/177 (45.20%), Query Frame = 0
Query: 598 PKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFK----NYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQE 770
P +PV+ + P F I IL+N L + + E VIFT +T E K+ F +Y D WN DFI+ I +A I G ++ ++ R FRV+R +K ++ G++T++ +S + L V IL ++ ++G+Q++ + Q+
Sbjct: 105 PFSPVRRVAIHILVHPFFSVFIITTILLNCLMMIKTSNERIESS-------EVIFTAIYTFESATKVMGRGFILCPFSYLRDAWNWLDFIV-------ITLAYITMGIDLGNLAVLRTFRVLRALKTVAIIPGLKTIVGAVIESVKNLRDVIILTVFSLSVFALLGLQIYMGVLTQK 267
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Match: EMLSAG00000008332 (supercontig:LSalAtl2s:LSalAtl2s508:183886:232439:1 gene:EMLSAG00000008332 transcript:EMLSAT00000008332 description:"maker-LSalAtl2s508-snap-gene-1.28") HSP 1 Score: 169.088 bits (427), Expect = 1.286e-46 Identity = 108/291 (37.11%), Postives = 154/291 (52.92%), Query Frame = 0
Query: 811 GVLCDEKSEDAGDPAG--------CGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSI--KTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDE--VTVGKFYATFLIQDYF--RRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGN 1087
G CD K+ + +G CG+ Y YFISF CSF++LNLFVAVIMDNFDYLTRD SILG HHL EF+ WSE+DP GRI + +V LLR I PPLGFGK CP R+A K +L+ MNMP++++G V+F TLFAL+R L I + +D+A+ ELR I+K W T +D + P A + +TVGK Y +I + + +F + + + + +++ ++ G+ L + P +++ GN
Sbjct: 7 GRSCDLKALEYNITSGTLINQEQNCGSSLTYIYFISFIFFCSFIMLNLFVAVIMDNFDYLTRDTSILGSHHLGEFITTWSEFDPSGIGRIHYTEVFNLLRSIDPPLGFGKKCPERMAFK--------------------------------KLIRMNMPMDTEGKVHFTTTLFALIREHLKINVRESSEMDQADIELRTTIIKCWPHTKRDKIDLLVPTAKSTGKGYLTVGKLYGGVMIHNNWCNTKFGQIEVLRRQEAELEALQKASDIKNGIIVLEDTSPSIEKLEKGN 265
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|32172454|sp|Q24270.2|CAC1D_DROME (RecName: Full=Voltage-dependent calcium channel type D subunit alpha-1; Short=DmCa1D) HSP 1 Score: 1457.97 bits (3773), Expect = 0.000e+0 Identity = 807/1376 (58.65%), Postives = 944/1376 (68.60%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDG-----ETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEI----------------------NPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKN------RRP---------------GAPNVSLNVN-----------------------SVQHAKISPTNSMDYAAQNSFRKMPDE--------DFPSDAANIQMRPVKLANGGSQD--------------HSRPDYQYLHPYYNANGDMVAPGGSGS------KSVPSSPSDRKQ---SMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGSL 1287
+ +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN+D +K R NFD F+Q+L+TVFQI+TGEDWN VMY GI A+GGV S G L+ +YFIILFICGNYILLNVFLAIAVDNLADAD+L + EK+E+ E DG I M+Q DD D+ D + + E +++ + RPR RM + +PIP ++FFLF N+FR+ CH++CNHS FGNI+L CIM SSAMLAAE+PL +N + NK+LN FDYFFT +FT+E+ LK+I+YGF+LH GAFCRS FNLLDLLVV VSLIS V SS AISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQLFKGKFF CTD SKMT++ C G ++ Y DGD++ P + ER W N FH+D+VAKG+LTLF VSTFEGWPG+LYVSIDSN+E+ GPIH FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNC+LDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKFY QP YT LD LN+IFT F LEF+FKL AFRFKNYFGD WN FDFIIVLGS IDI +EI + G N+ISINFFRLFRVMRLVKLLSKGEGIRTLLWTF KSFQALP+VA+LI L+FFIY VVGMQVFGKIA G I NNNFQTF QA+LVLFRSATGEAWQEIM+SC P V CD S+ G+P CG+ AYPYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHR+ACK RLVSMNMPLNSDG+V FNATLFA+VRTSLSIKT+GNID+AN ELR I ++W+RT+ KLLDQ+ P G +DEVTVGKFYAT+LIQDYFRRFKK+KE + HPD +VTLQAGLRTLHE P LKRAISGNLDE L E E MHRR+ FG+V SSI+ RR G+ + +L V SV + T ++ A + K+ DE + + +I MRP+ GG+ +R LHPY N + AP G+ +S P+SP D+++ S + G AE+L+G VL +GLGKYCD +FV A+REM+EA+DMTPEE + AAH++L E SL
Sbjct: 1091 VTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEEEPHDESAQKKSHSPTPTIDGMDDHLSIDIDMEQQELDDEDKMD-------HETLSDEEVREMCEEEEEVDEEGMITARPR-------------------------RMSEVNTATKILPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSATGEAWQEIMMSCSAQPDVKCDMNSDTPGEP--CGSSIAYPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQVVPPPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEGHPD--SNTVTLQAGLRTLHEVSPALKRAISGNLDE------LDQEPEPMHRRHHTLFGSVWSSIRRHGNGTFRRSAKATASQSNGALAIGGSASAALGVGGSSLVLGSSDPAGGDYLYDTLNRSVADGVNNITRNIMQARLAAAGKLQDELQGAGSGGELRTFGESISMRPLAKNGGGAATVAGTLPPEANAINYDNRNRGILLHPYNN----VYAPNGALPGHERMIQSTPASPYDQRRLPTSSDMNGLAESLIGGVLAAEGLGKYCDSEFVGTAAREMREALDMTPEEMNLAAHQILSNEHSLSL 2388 HSP 2 Score: 206.453 bits (524), Expect = 3.256e-52 Identity = 171/666 (25.68%), Postives = 288/666 (43.24%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEE------------------TCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTF-------QNEG--ESAYKNCELDKNQRNCIEFALNAKPVR--------------------------RYIPKNPVQYKLW-------------------AFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNM--ISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
A F N R +C I F ++L+ I + LA P + ++ N+ L +Y F IFT E +K++AYGF+LH GA+ R+G+NLLD +V + IS S VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK R ++T E C G + DG N+ DN +LT+F T EGW +LY D+ D YFI ++I+ AFF++N+ +G + F +N G + + +++++ R +++ A+ + +P+ + W V S F + I ++ +NT LA + Y Q + F + NV F FT E + K+ + F+ YF +N FD +V+GS+ + + G M + ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +G NF F QALL +F+ TGE W +M G + + A YFI F+ ++++LN+F+A+ +DN
Sbjct: 594 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC-RDEITGEYEENIRPCGVGYQCPPGYKCYGGWDGPNDGITNF---------------DNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDW----------QWMYFISMVILGAFFVMNLILGVLSGEFSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNT---GMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGA------------LACIYFIILFICGNYILLNVFLAIAVDNL 1218
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|5921703|sp|Q25452.1|CAC1M_MUSDO (RecName: Full=Muscle calcium channel subunit alpha-1; AltName: Full=MDL-alpha-1) HSP 1 Score: 1228 bits (3176), Expect = 0.000e+0 Identity = 639/1008 (63.39%), Postives = 755/1008 (74.90%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDE------DDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQY--DEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP------------------GGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACK------------------------------IKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMN 959
LL + +YW +LSNLV SL NSIQSIASLLLLLFLF+ IF LLGMQVFG RF + P +KPR NFD+F+QSL+TVFQILTGEDWNVVMYDGI+A+GGV S GI++ +Y+IILFICGNYILLNVFLAIAVDNLADAD+L EK++ D+ ++ ++EE G+ I + D D Y DE +Y D E+ Y + V QRV+ PR + + DR+++ IP ++FF+F H N+FRI CH +CNHS FGN +L CIM SSAMLAAE+PL +++ RN +LN FDYFFT +FT+E+ LK+I+YGF+LH GAFCRS FNLLDLLVV VSLIS F+S AISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQLFKGKFF C+D SK+ E C G ++ Y++GD+N P ++ER W N FH+D+VAK +LTLF VSTFEGWP +LYVSIDSN+E+ GPI+ FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEY+IF +I++NT++LAMKFY+QPE Y+ LD LN+IFT F+LEFIFKL AFRFKNYFGD WN FDFIIVLGS IDI +EI G +ISINFFRLFRVMRLVKLLSKGEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKI +EGT+I RNNNFQTFPQA+LVLFRSATGEAWQEIM++C V CD +S+ + CG+ A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHR+ACK IK +G ID+AN EL+ + +IWKRT +++D +++ N
Sbjct: 534 LLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEEKPRSNFDSFYQSLLTVFQILTGEDWNVVMYDGIRAYGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDESQIQLDNQIKNEMENEEYLQNGD----HISFKAEFGADLDTYLQDEECGSYSDD------ENTYNKLGG---VKQRVSSLPRR--------------NTNTDMDRIKK-----------DIPYGTSFFIFSHTNRFRIFCHRLCNHSNFGNFILCCIMFSSAMLAAENPLKADASRNIVLNKFDYFFTAVFTIELVLKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISIFFNSNAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFSCSDGSKVYESDCHGTYLFYENGDINKPRLKEREWKNNKFHFDDVAKAMLTLFTVSTFEGWPTLLYVSIDSNKENGGPIYNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHSIQYKVWWFVTSSSFEYSIFVLIMINTVTLAMKFYKQPEYYSEILDALNMIFTAVFSLEFIFKLAAFRFKNYFGDAWNTFDFIIVLGSFIDIVYSEIKTKEQALATCDGQSCNKAKGGSTLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIMLEEGTSIDRNNNFQTFPQAVLVLFRSATGEAWQEIMMACSPRDDVKCDPESDAVNN---CGSSIAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRLPLAIKTDGNIDEANAELRATIKQIWKRTNPRLLDQVVLTGN 1500 HSP 2 Score: 129.413 bits (324), Expect = 1.009e-28 Identity = 95/321 (29.60%), Postives = 152/321 (47.35%), Query Frame = 0
Query: 269 PEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSER--NKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTE-----ETCQGQFIEYKDGDLNYPIVEERIWDRNAF-HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
PE A F N RI C I + F +L+ I + LA P S N++L +Y F IFT E +K+IAYGF+LH G++ R+G+N LD +V + +IS S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK S E + + +K Y ++ + + ++DN +LT+F T EGW +LY D+ I YF+ ++I+ AFF++N+ +G + F E A + K
Sbjct: 37 PE-RALFCLTLKNPLRIFCIKIVDSKLFEYFILLTIFANCVALAVYTPYPSGDSNITNQMLEKIEYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTALSNLVKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPS-GYECFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSSWEWI----------YFVSMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQK 345 HSP 3 Score: 104.375 bits (259), Expect = 4.110e-21 Identity = 75/259 (28.96%), Postives = 127/259 (49.03%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAE---INPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFG---KIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDF---AYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT LA + YRQP F + N+ F FT E I K+ + F+ YF +N FD +V+GS+ ++ + + P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+G++GMQVFG P+E +NF +F Q+LL +F+ TGE W +M D G F A Y+I F+ ++++LN+F+A+ +DN
Sbjct: 431 VKSQAFYWLIILLVFLNTGVLATEHYRQPIWLDQFQEYTNIFFIALFTCEMILKMYSLGFQGYFVSLFNRFDCFVVIGSISEMVLTSSELMAPLG----VSVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTFKPEEE---KPRSNFDSFYQSLLTVFQILTGEDWNVVMY---------------DGIRAYGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNL 667 HSP 4 Score: 89.7373 bits (221), Expect = 1.167e-16 Identity = 129/613 (21.04%), Postives = 244/613 (39.80%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARF--------------NYDPLA------QKPRG---------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI---------VTFQNEGESAYKNCELDKNQRNCIE 585
+L ++NS+ ++ + + LL+ + I+A++G+++F + + D L + P G NFDNF S++TVFQ +T E W V+Y A G +YF+ + I G + ++N+ L + L E E ++ D ++ ++ Q +E R Y + +ED + DP + + K +S D + +E + D R + +L + + + R C F ++++ + +++ +LA E ++ Y + FF +FT E+ LK+ + GF G F S FN D VV S+ V +S + V +LR +R+LR + L ++V ++ ++++I +++L+ L +F ++G+Q+F G+F K EE + F D+ + LLT+F + T E W ++Y I + +G + F + I+Y I I + ++N+F+ + T + E ES +LD +N +E
Sbjct: 179 SLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSIQDAMGSS-----WEWIYFVSMVILGAFFVMNLILGV----------LSGEFSKERTKAKNRGDFQKLREK------------------QQIEEDLRGYLD--WITQAED--IEPDPDAQIIEDCHKNKVKEVVS--IDNLKDHENETQQTDSWFR---------------SQKKYLERINRRIRRACRKAVKSQAFYWLIILLVFLNTGVLATEH-YRQPIWLDQFQEYTNIFFIALFTCEMILKMYSLGF---QGYFV-SLFNRFDCFVVIGSISEMVLTSSELMAPLGVSVLRCVRLLRVFKVTKYWHSLSNLVASLLNSIQSIASLLLLLFLFIVIFGLLGMQVFGGRFTF-----KPEEEKPRSNF-------------------------DSFYQSLLTVFQILTGEDWNVVMYDGIRA----YGGVFSFGIVACIYYIILFICGNYILLNVFLAIAVDNLADADSLSTIEKEDES---QIQLDNQIKNEME 695 HSP 5 Score: 89.3521 bits (220), Expect = 1.517e-16 Identity = 74/304 (24.34%), Postives = 133/304 (43.75%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEG----YTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFG----KIAPQEGTNIHRNN-------------------------------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
KNP++ V S +FEY I I N ++LA+ + P G L+ + IF V FT E + K+ A+ F +Y + WN DF IV+ +I A++ + G + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F K T + N+ NF F ++L +F+ T E W +++ S +DA G+ + + YF+S ++ +F ++NL + V+ F
Sbjct: 47 KNPLRIFCIKIVDSKLFEYFILLTIFANCVALAV-YTPYPSGDSNITNQMLEKIEYIFLVIFTSECVMKIIAYGFVLHTGSYLRNGWNFLDFFIVVIGMISTALSNLVKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMIPLLHIALLVLFVIIIYAIIGLELFSGKLHKTCRHSNTGEYLNDLDELHACGVGFKCPSGYECFDDWVGPNDGITNFDNFGLSMLTVFQCITLEGWTDVLYSI------------QDA-----MGSSWEWIYFVSMVILGAFFVMNLILGVLSGEF 330
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|1209676875|sp|Q02485.2|CAC1S_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=ROB1; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 1201.04 bits (3106), Expect = 0.000e+0 Identity = 654/1165 (56.14%), Postives = 819/1165 (70.30%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGD-----RMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP---------------------GGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDA-GDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEK-GPELKRAISGNL---DEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKI 1141
LL I +YW +LSNLV SL NSI+SIASLLLLLFLFM IFALLGMQ+FG R++++ + R NFDNF Q+LI+VFQ+LTGEDWN VMY+GI A+GG G+L +YFIILF+CGNYILLNVFLAIAVDNLA+A++L +K + + + D E+ + + + ++ + G A DE+ + + ++V + P S +F G DEEDE + R R + +PIPEAS+FF+F N+ R++CH I N ++F N +L+ I++SSA LAAEDP+ ++S RN+IL YFDY FT +FTVE+ LK+ YG LH G+FCR+ FN+LDLLVVAVSLIS S AISVVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNIVLVT LLQFMFA IGVQLFKGKF+ C D SKMTEE C+G + YKDGD + R W N FH+DNV +++LF VSTFEGWP +LY +IDSNEED GP++ R +AIF+ IYII+IAFFM+NIFVGFVIVTFQ +GE+ YKNCELDKNQR C+++AL A+P+R YIPKNP QY++W VTS FEY +FA+I++NT+ L M+ Y Q E D+LNV FT+ FTLE I KL AF+ + YFGDPWN FDF+IV+GS+ID+ ++EI+ IS FFRLFRVMRL+KLLS+ EG+RTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA +GT I+RNNNFQTFPQA+L+LFR ATGEAWQEI+L+C G CD +S+ A G+ CGT+FAY YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF +W+EYDP+AKGRIKH+DVVTLLR+I PPLGFGK CPHRVACK RLV MNMPLNSDG+V FNATLFALVRT+L IKTEGN ++ANEELR I K+W+RT +KLLDQ+ P G +DEVTVGKFYATFLIQ++FR+F K++E + P ++ +V +QAGLRT+ E+ PE+ RAISG+L +E+ AM EE + RR FG V + R P V N +Q A++
Sbjct: 532 LLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVR-RSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMT---------KKLEQKPKGEGIPTTAKLKIDEF-ESNVNEV---------KDPYPSADFPG----DDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSY--GKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACK--------------------------------RLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIG-DDEVTVGKFYATFLIQEHFRKFMKRQE-EYYGYRP--KKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQ-VDNFLERTNSLPPVMANQRPLQFAEM 1633 HSP 2 Score: 224.557 bits (571), Expect = 6.065e-58 Identity = 183/698 (26.22%), Postives = 316/698 (45.27%), Query Frame = 0
Query: 247 EDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF--------------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGK-----FFMCTDRSKMTEET-------------CQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESA---------YKNCELDKNQRNCIEFALNAKP-----------------------------------VRRYIPKNPV-QYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNM--ISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
+DEG +R+ + PV +P P A F N R C + F I+L+ I + LA P+ + N+ N L +YFF +F++E +K+IAYGF+ H A+ RSG+N+LD ++V + + + + S GA VK LR RVLRPLR ++ L+ V+ + A+ + +I L+ L + ++A+IG++LFKGK +F+ TD E C E + G W + H+DN +LT++ + EGW +LY D+ + I YF+ +I++ +FF++N+ +G + F E E A + +L+++ R + + + +R + N V ++K V S +F + + ++ +NTLS+A + + QP T DV N + FT+E + K+ + YF +N FD +V +++I + E G M + I+ R R++RL K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG E T + R +NF FPQAL+ +F+ TGE W +M + G++ G P+ G YFI FV ++++LN+F+A+ +DN
Sbjct: 7 QDEG--LRKKQPKKPVPEILPRP-PRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGG-----------WPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWI----------YFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVE---SGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRR-SNFDNFPQALISVFQVLTGEDWNSVMYN-----GIMA------YGGPSYPGV-LVCIYFIILFVCGNYILLNVFLAIAVDNL 664
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|6165982|sp|Q01815.1|CAC1C_MOUSE (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=MELC-CC; AltName: Full=Mouse brain class C; Short=MBC; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 1198.34 bits (3099), Expect = 0.000e+0 Identity = 664/1184 (56.08%), Postives = 823/1184 (69.51%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKD-----------EDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPG-------------GNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAG---CGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNL-------DEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDF 1163
I RYW +LSNLV SL NS++SIASLLLLLFLF+ IF+LLGMQ+FG +FN+D + Q R FDNF QSL+TVFQILTGEDWN VMYDGI A+GG G+L +YFIILFICGNYILLNVFLAIAVDNLADA++L +K+ PE + E E E KI + D + + D +ED+ +P G+ + P P G R R + +P+PEASAFF+F +N+FR+ CH I N + F N++L I++SS LAAEDP+ S RN IL DY FT+IFT+E+ LK+ AYG LH G+FCR+ FN+LDLLVV+VSLISF S AI+VVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNIV+VT LLQFMFA IGVQLFKGK + C+D SK TE C+G +I YKDG++++PI++ R W+ + F +DNV ++ LF VSTFEGWP +LY SIDS+ ED GPI+ +R ++IF+ IYIIIIAFFM+NIFVGFVIVTFQ +GE YKNCELDKNQR C+E+AL A+P+RRYIPKN QYK+W V S FEY +F +IL+NT+ LAM+ Y Q + +++LN++FT FT+E I KL AF+ K YF DPWN FDF+IV+GS+ID+ ++E NP + ISI FFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA + T I+RNNNFQTFPQA+L+LFR ATGEAWQ+IML+C PG C +SE + G CG+ FA YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF R+W+EYDP+AKGRIKH+DVVTLLR+I PPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFALVRT+L IKTEGN+++ANEELR I K+W+RT +KLLDQ+ P AG +DEVTVGKFYATFLIQ+YFR+FKK+KE + L P + +++LQAGLRTLH+ GPE++RAISG+L M EA+ +E++ + RR FGN V+ ++ G + H + N D + S K+ D F
Sbjct: 630 ITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEM-QTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADG--ESPPTTKINMDDLQPSENEDKSPHSNP-DTAGEEDEEEPEMPV-----------------GPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACM--PGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAG-DDEVTVGKFYATFLIQEYFRKFKKRKE-QGLVGKPS-QRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDD-IFRRAGGLFGNHVTYYQSDSRGNFPQTFATQRPLHINKTGNNQADTESP-SHEKLVDSTF 1753 HSP 2 Score: 132.88 bits (333), Expect = 9.461e-30 Identity = 103/338 (30.47%), Postives = 155/338 (45.86%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF-------------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCT-------------DRSKMTEETCQGQFIEYKDGDLNYPIVEERIWD---RNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A N R C I F I+L+ I + LA P + ++ N L +Y F IFTVE LKVIAYG + HP A+ R+G+NLLD ++V V L S + GA VK LR RVLRPLR ++ L+ V+ +I A+ + +I L+ L + ++A+IG++LF GK D S ET G+ + ++G + P WD ++DN A +LT+F T EGW +LY D+ G+ + YF+ ++I +FF++N+ +G + F E E A + K
Sbjct: 103 ALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGR--QCQNGTVCKP-----GWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDA--------MGYE--LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 423 HSP 3 Score: 98.9821 bits (245), Expect = 1.835e-19 Identity = 74/259 (28.57%), Postives = 129/259 (49.81%), Query Frame = 0
Query: 605 KLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAE---INPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
K A V S +F + + ++ +NTL++A + Y QP T D N FT E + K+ + + YF +N FD IV G +++ + E ++P G I+ R R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+F GK E R + F FPQ+LL +F+ TGE W +M G++ G P+ G YFI F+ ++++LN+F+A+ +DN
Sbjct: 516 KCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLG----ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQT--RRSTFDNFPQSLLTVFQILTGEDWNSVMYD-----GIMA------YGGPSFPGM-LVCIYFIILFICGNYILLNVFLAIAVDNL 756 HSP 4 Score: 80.4925 bits (197), Expect = 9.651e-14 Identity = 129/589 (21.90%), Postives = 246/589 (41.77%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYD----------PLAQKPR-----------------------------GNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLS-NEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQF-RIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLI------SFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIA-FFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + P + P NFDNF +++TVFQ +T E W V+Y A G L +YF+ L I G++ +LN+ L + + +E+++ D E + ED + Y D Q +++ +EDE +D R P S + S N + G D + E+ G R+ IS+S + ++ N+F R C + F +V+ + +++ +A+E N ++ + + +FT E+ LK+ + G A+ S FN D +V ++ + + S ISV++ +R+LR+ + R N L ++V ++ +V++I +++L+ L +F+++G+QLF GKF N+ ++ R R+ F DN + LLT+F + T E W ++Y I + GP F ++ YFI + I + ++N+F+ +
Sbjct: 253 SLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYE-----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFS-------KEREKAKARGDFQKLREKQQLEEDLKG-------YLDWITQAEDIDPE---------NEDEGMDEDKP-----RNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSR-----------YWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWN---SLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKF--------------------------NFDEMQTR---RSTF--DNFPQSLLTVFQILTGEDWNSVMYDGIMAY---GGP--SFPGMLVCIYFIILFICGNYILLNVFLAIAV 753 HSP 5 Score: 78.5666 bits (192), Expect = 3.251e-13 Identity = 77/318 (24.21%), Postives = 131/318 (41.19%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPE----GYTAFLDVLNVIFTVFFTLEFIFKLGA----FRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP--GGNMIS-------INFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKI-------------------AP-----------QEGTNI--------HRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 860
KNP++ + V FE I I N ++LA+ + PE + L+ + +F + FT+E K+ A F Y + WN DFIIV+ L + + G N + + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F GK+ +P Q GT H NF F A+L +F+ T E W +++ +DA G + + YF+S + SF +LNL + V+ F
Sbjct: 110 KNPIRRACISIVEWKPFEIIILLTIFANCVALAI-YIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWM------------QDA-----MGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFS 409
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|209572767|sp|Q13698.4|CAC1S_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 1197.19 bits (3096), Expect = 0.000e+0 Identity = 689/1373 (50.18%), Postives = 883/1373 (64.31%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGD-----RMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP---------------------GGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDA-GDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQS--VTLQAGLRTLHEK-GPELKRAISGNL---DEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKI------SP----------------------------------TNSMDYAAQNSFRKMPDE-DFPSDAANIQMRPVKLANGGSQDHSRPDYQYLHPYYNANG---DMVAPGGSGSKSVPSS-PSDRKQSMP---------IVGSAEN-----------LVGRVLRDQGLGKY-CDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQA 1281
LL I +YW +LSNLV SL NSI+SIASLLLLLFLF+ IFALLGMQ+FG R++++ + R NFDNF Q+LI+VFQ+LTGEDW +MY+GI A+GG G+L +YFIILF+CGNYILLNVFLAIAVDNLA+A++L +K + + + + D E+ ++ + + ++ + G A DE+ + + ++V + P S +F G DEEDE + R R + +PIPEAS+FF+F N+ R++CH I N ++F N +L+ I++SSA LAAEDP+ ++S RN+IL +FD FT++FTVE+ LK+ YG LH G+FCR+ FN+LDLLVVAVSLIS S AISVVKILRVLRVLRPLRAINRAKGLKHVVQC+ VA+ TIGNIVLVT LLQFMFA IGVQLFKGKFF CTD SKMTEE C+G + YKDGD + R W + FH+DNV +++LF VSTFEGWP +LY +IDSN ED GPI+ R +AIF+ IYII+IAFFM+NIFVGFVIVTFQ +GE+ YKNCELDKNQR C+++AL A+P+R YIPKNP QY++W VTS FEY +FA+I++NT+ L M+ Y Q E D+LNV FT+ FTLE I KL AF+ + YFGDPWN FDF+IV+GS+ID+ ++EI+ IS FFRLFRVMRL+KLLS+ EG+RTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA +GT I+RNNNFQTFPQA+L+LFR ATGEAWQEI+L+C G LCD +S+ A G+ CGT+FAY YFISF+++C+FL++NLFVAVIMDNFDYLTRDWSILGPHHLDEF +W+EYDP+AKGRIKH+DVVTLLR+I PPLGFGK CPHRVACK RLV MNMPLNSDG+V FNATLFALVRT+L IKTEGN ++ANEELR I K+W+RT +KLLDQ+ P G +DEVTVGKFYATFLIQ++FR+F K++E E+ + + V +QAGLRT+ E+ PE+ R +SG+L +E+ AM EE + RR FG V + R P V N +Q A+I SP +NS +++ + R+ P+E + P+ +P ++ HS+P + L P V SS P DRK S P + EN L+ + L GLG D +F+ A + + +A M PEE + A LL+
Sbjct: 532 LLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVR-RSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMA---------KKLEQKPKGEGIPTTAKLKIDEF-ESNVNEV---------KDPYPSADFPG----DDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSY--GKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACK--------------------------------RLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIG-DDEVTVGKFYATFLIQEHFRKFMKRQE-----EYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQ-VDNFLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLG----PHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSMPEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKG 1835 HSP 2 Score: 222.246 bits (565), Expect = 2.835e-57 Identity = 183/698 (26.22%), Postives = 315/698 (45.13%), Query Frame = 0
Query: 247 EDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF--------------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGK-----FFMCTDRSKMTE-------------ETCQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESA---------YKNCELDKNQRNCIEFALNAKP-----------------------------------VRRYIPKNPV-QYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNM--ISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
+DEG +R+ + PV +P P A F +N R C I F I+L+ I + LA P+ + N+ N L +YFF +F++E +K+IAYGF+ H A+ RSG+N+LD +V + + + + S GA VK LR RVLRPLR ++ L+ V+ + A+ + +I L+ L + ++A+IG++LFKGK +F+ TD E C E + G W + H+DN +LT++ T EGW +LY D+ + I YF+ +I++ +FF++N+ +G + F E E A + +LD++ R + + + +R + N + ++K V S +F + + ++ +NTLS+A + + QP T D+ N + FT E + K+ + YF +N FD +V +++I + E G M + I+ R R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG E T + R +NF FPQAL+ +F+ TGE W +M + G++ G P+ G YFI FV ++++LN+F+A+ +DN
Sbjct: 7 QDEG--LRKKQPKKPVPEILPRP-PRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGG-----------WPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWI----------YFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVE---SGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRR-SNFDNFPQALISVFQVLTGEDWTSMMYN-----GIMA------YGGPSYPGM-LVCIYFIILFVCGNYILLNVFLAIAVDNL 664
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|116411|sp|P15381.1|CAC1C_RABIT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1C; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; AltName: Full=Smooth muscle calcium channel blocker receptor; Short=CACB-receptor; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.2) HSP 1 Score: 1196.42 bits (3094), Expect = 0.000e+0 Identity = 674/1201 (56.12%), Postives = 829/1201 (69.03%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALED--------------------EEKDE--DDPP--EDGDDDEEGKDGGEDGE----TKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPG-------------GNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAG---CGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNL-------DEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDF 1163
I RYW +LSNLV SL NS++SIASLLLLLFLF+ IF+LLGMQ+FG +FN+D + Q R FDNF QSL+TVFQILTGEDWN VMYDGI A+GG G+L +YFIILFICGNYILLNVFLAIAVDNLADA++L E+K E P E ++ E K DGE TKI MD P++ +D P+ + ++D + V RPR S + E V P+PEASAFF+F +N+FR+ CH I N + F N++L I++SS LAAEDP+ S RN IL YFD FTTIFT+E+ LK+ AYG LH G+FCR+ FN+LDLLVV+VSLISF S AI+VVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNIV+VT LLQFMFA IGVQLFKGK + C+D SK TE C+G +I YKDG++++PI++ R W+ + F +DNV ++ LF VSTFEGWP +LY SIDS+ ED GPI+ +R ++IF+ IYIIIIAFFM+NIFVGFVIVTFQ +GE YKNCELDKNQR C+E+AL A+P+RRYIPKN QYK+W V S FEY +F +IL+NT+ LAM+ Y Q + +++LN++FT FT+E I KL AF+ K YF DPWN FDF+IV+GS+ID+ ++E NP + ISI FFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA + T I+RNNNFQTFPQA+L+LFR ATGEAWQ+IML+C PG C +SE G CG+ FA YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF R+W+EYDP+AKGRIKH+DVVTLLR+I PPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFALVRT+L IKTEGN+++ANEELR I K+W+RT +KLLDQ+ P AG +DEVTVGKFYATFLIQ+YFR+FKK+KE + L P + +++LQAGLRTLH+ GPE++RAISG+L M EA+ +E++ + RR FGN VS ++ A + H + N D + S K+ D F
Sbjct: 660 ITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEM-QTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMDDLQPNE-----------SEDKSPYPNPETTGEED-EEEPEMPVGPRPRPLS---------------------ELHLKEKAV----PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACM--PGKKCAPESEPHNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAG-DDEVTVGKFYATFLIQEYFRKFKKRKE-QGLVGKPS-QRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDD-IFRRAGGLFGNHVSYYQSDSRSAFPQTFTTQRPLHISKAGNNQGDTESP-SHEKLVDSTF 1783 HSP 2 Score: 132.88 bits (333), Expect = 9.197e-30 Identity = 103/338 (30.47%), Postives = 155/338 (45.86%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF-------------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCT-------------DRSKMTEETCQGQFIEYKDGDLNYPIVEERIWD---RNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A N R C I F I+L+ I + LA P + ++ N L +Y F IFTVE LKVIAYG + HP A+ R+G+NLLD ++V V L S + GA VK LR RVLRPLR ++ L+ V+ +I A+ + +I L+ L + ++A+IG++LF GK D S ET G+ + ++G + P WD ++DN A +LT+F T EGW +LY D+ G+ + YF+ ++I +FF++N+ +G + F E E A + K
Sbjct: 133 ALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGR--QCQNGTVCKP-----GWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDA--------MGYE--LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 453 HSP 3 Score: 98.9821 bits (245), Expect = 2.255e-19 Identity = 74/259 (28.57%), Postives = 129/259 (49.81%), Query Frame = 0
Query: 605 KLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAE---INPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
K A V S +F + + ++ +NTL++A + Y QP T D N FT E + K+ + + YF +N FD IV G +++ + E ++P G I+ R R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+F GK E R + F FPQ+LL +F+ TGE W +M G++ G P+ G YFI F+ ++++LN+F+A+ +DN
Sbjct: 546 KCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKVMSPLG----ISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQT--RRSTFDNFPQSLLTVFQILTGEDWNSVMYD-----GIMA------YGGPSFPGM-LVCIYFIILFICGNYILLNVFLAIAVDNL 786 HSP 4 Score: 80.4925 bits (197), Expect = 9.516e-14 Identity = 130/589 (22.07%), Postives = 247/589 (41.94%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGAR-----FNYDPLAQKPR----------------------------------GNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLS-NEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQF-RIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLI------SFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIA-FFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + +N + +A P NFDNF +++TVFQ +T E W V+Y A G L +YF+ L I G++ +LN+ L + + +E+++ D E + ED + Y D Q +++ +EDE ++ R P S + S N + G D + E+ G R+ IS+S + ++ N+F R C + F +V+ + +++ +A+E N ++ + + +FT E+ LK+ + G A+ S FN D +V ++ + V S ISV++ +R+LR+ + R N L ++V ++ +V++I +++L+ L +F+++G+QLF GKF N+ ++ R R+ F DN + LLT+F + T E W ++Y D GP F ++ YFI + I + ++N+F+ +
Sbjct: 283 SLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYE-----LPWVYFVSLVIFGSFFVLNLVLGVLSGEFS-------KEREKAKARGDFQKLREKQQLEEDLKG-------YLDWITQAEDIDPE---------NEDEGMDEEKP-----RNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSR-----------YWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEH-YNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGL----QAYFVSLFNRFDCFIVCGGILETILVETKVMSPLGISVLRCVRLLRIFKITRYWN---SLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKF--------------------------NFDEMQTR---RSTF--DNFPQSLLTVFQILTGEDWNSVMY---DGIMAYGGP--SFPGMLVCIYFIILFICGNYILLNVFLAIAV 783 HSP 5 Score: 78.5666 bits (192), Expect = 3.233e-13 Identity = 77/318 (24.21%), Postives = 131/318 (41.19%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPE----GYTAFLDVLNVIFTVFFTLEFIFKLGA----FRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP--GGNMIS-------INFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKI-------------------AP-----------QEGTNI--------HRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFD 860
KNP++ + V FE I I N ++LA+ + PE + L+ + +F + FT+E K+ A F Y + WN DFIIV+ L + + G N + + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F GK+ +P Q GT H NF F A+L +F+ T E W +++ +DA G + + YF+S + SF +LNL + V+ F
Sbjct: 140 KNPIRRACISIVEWKPFEIIILLTIFANCVALAI-YIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWM------------QDA-----MGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFS 439
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|341940563|sp|Q99246.3|CAC1D_MOUSE (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=Calcium channel, L type, alpha-1 polypeptide isoform 2; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 1196.42 bits (3094), Expect = 0.000e+0 Identity = 652/1139 (57.24%), Postives = 812/1139 (71.29%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKD-----------EDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPG-----------------GNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSE-DAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRR 1123
+ R+W +LSNLV SL NS++SIASLLLLLFLF+ IF+LLGMQ+FG +FN+D Q R FDNF Q+L+TVFQILTGEDWN VMYDGI A+GG S G++ +YFIILFICGNYILLNVFLAIAVDNLADA++L +K+ + E+ +++ + + + K+ +D Y D D+ D Y P + E + D E G R RR+ + PIPE SAFF+ N R+ CH + NH F N++LV IM+SSA LAAEDP+ S+S RN IL YFDY FT IFTVE+ LK+ +G LH GAFCR+ FNLLD+LVV VSL+SF S AISVVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNI++VT LLQFMFA IGVQLFKGKF+ CTD +K E C+G FI YKDGD++ P+V ERIW + F++DNV ++ LF VSTFEGWP +LY +IDSN E+ GP++ +R ++IF+ IYIII+AFFM+NIFVGFVIVTFQ +GE YKNCELDKNQR C+E+AL A+P+RRYIPKNP QYK W V S FEY +F +I++NTL LAM+ Y Q + + +D+LN++FT FT+E + K+ AF+ K YF D WN FD +IV+GS+ID+A++E +P N ISI FFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGK+A ++ I+RNNNFQTFPQA+L+LFR ATGEAWQEIML+C PG LCD S+ + G+ CG++FA YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF R+WSEYDP+AKGRIKH+DVVTLLR+I PPLGFGKLCPHRVACK RLV+MNMPLNSDG+V FNATLFALVRT+L IKTEGN+++ANEELR I K+W++T +KLLDQ+ P AG +DEVTVGKFYATFLIQDYFR+FKK+KE + ++P + ++ LQAGLRTLH+ GPE++RAIS +L + E D EEE + +RN GN V+ + + R
Sbjct: 649 VTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDE-TQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDK----------DPYP-----------------------PCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPSESETIPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACL--PGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK--------------------------------RLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAG-DDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYP-AKNTTIALQAGLRTLHDIGPEIRRAISCDLQD-DEPEDSKPEEEDVFKRNGALLGNHVNHVNSDR 1716 HSP 2 Score: 131.339 bits (329), Expect = 2.885e-29 Identity = 110/344 (31.98%), Postives = 158/344 (45.93%), Query Frame = 0
Query: 271 ASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL---NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF------------SSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF-MC--TDRSKMTEE------------TCQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A A F +N R C I F +L+ I + LA P +SNS N L +Y F IFTVE LK+IAYG +LHP A+ R+G+NLLD ++V V L S + SSG VK LR RVLRPLR ++ L+ V+ +I A+ + +I L+ L + ++A+IG++LF GK C D + EE C E + G W + ++DN A +LT+F T EGW +LY D+ I P V YF+ +II+ +FF++N+ +G + F E E A + K
Sbjct: 103 ARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNST-NHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSG-----------WVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDA-------IGWEWPWV---YFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 424 HSP 3 Score: 105.916 bits (263), Expect = 1.616e-21 Identity = 71/255 (27.84%), Postives = 130/255 (50.98%), Query Frame = 0
Query: 608 AFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEI---NPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
A V S F + + ++ +NTL+++ + Y QP+ T D+ N + FT E + K+ + + YF +N FD +V G + + + E+ +P G ++ FR R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG + T R + F FPQALL +F+ TGE W +M G++ G P+ G YFI F+ ++++LN+F+A+ +DN
Sbjct: 538 AAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLG----VSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKR-STFDNFPQALLTVFQILTGEDWNAVMYD-----GIMA------YGGPSSSGMIVCI-YFIILFICGNYILLNVFLAIAVDNL 775 HSP 4 Score: 78.1814 bits (191), Expect = 4.610e-13 Identity = 72/329 (21.88%), Postives = 130/329 (39.51%), Query Frame = 0
Query: 589 NAKPVRRYIP---KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPE----GYTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINP---GGNMIS-------INFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-----------------------------GKIAPQEGTNIHRN--------NNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
N++P R NP++ + V F+ I I N ++LA+ + PE L+ + F + FT+E K+ A+ Y + WN DF+IV+ L + + ++ GGN S + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F G+ GT NF F A+L +F+ T E W +++ G ++ + YF+S ++ SF +LNL + V+ F
Sbjct: 99 NSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAI-YIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVND-----------------AIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEF 409
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|6165987|sp|P27732.2|CAC1D_RAT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 2; AltName: Full=Rat brain class D; Short=RBD; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 1195.26 bits (3091), Expect = 0.000e+0 Identity = 648/1124 (57.65%), Postives = 811/1124 (72.15%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKD-----------EDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPG--GNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSE-DAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRR 1123
+ R+W +LSNLV SL NS++SIASLLLLLFLF+ IF+LLGMQ+FG +FN+D Q R FDNF Q+L+TVFQILTGEDWN VMYDGI A+GG S G++ +YFIILFICGNYILL +FLAIAVDNLADA++L +K+ + E+ +++ + + + K+ +D Y ++E +DP P + E + D E G R RR+ + PIPE SAFF+ N R+ CH + NH F N++LV IM+SSA LAAEDP+ S+S RN IL YFDY FT IFTVE+ LK+ +G LH GAFCR+ FNLLD+LVV VSL+SF S AISVVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNI++VT LLQFMFA IGVQLFKGKF+ CTD +K E C+G FI YKDGD++ P+V ERIW + F++DNV ++ LF VSTFEGWP +LY +IDSN E+ GP++ +R ++IF+ IYIII+AFFM+NIFVGFVIVTFQ +GE YKNCELDKNQR C+E+AL A+P+RRYIPKNP QYK W V S FEY +F +I++NTL LAM+ Y Q + + +D+LN++FT FT+E + K+ AF+ K YF D WN FD +IV+GS+ID+A++E + N ISI FFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGK+A ++ I+RNNNFQTFPQA+L+LFR ATGEAWQEIML+C PG LCD S+ + G+ CG++FA YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF R+WSEYDP+AKGRIKH+DVVTLLR+I PPLGFGKLCPHRVACK RLV+MNMPLNSDG+V FNATLFALVRT+L IKTEGN+++ANEELR I K+W++T +KLLDQ+ P AG +DEVTVGKFYATFLIQDYFR+FKK+KE + ++P + ++ LQAGLRTLH+ GPE++RAIS +L + E D EEE + +RN GN V+ + + R
Sbjct: 688 VTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDE-TQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLKLFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDY------------------------QEEAEDKDPYP---------PCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACL--PGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK--------------------------------RLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAG-DDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYP-AKNTTIALQAGLRTLHDIGPEIRRAISCDLQD-DEPEDSKPEEEDVFKRNGALLGNYVNHVNSDR 1740 HSP 2 Score: 126.331 bits (316), Expect = 1.036e-27 Identity = 109/344 (31.69%), Postives = 157/344 (45.64%), Query Frame = 0
Query: 271 ASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL---NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF------------SSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF-MC--TDRSKMTEE------------TCQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A A F +N R C I + F +L+ I + LA P +SNS N L +Y F IFTVE LK+IA G +LHP A R+G+NLLD ++V V L S + SSG VK LR RVLRPLR ++ L+ V+ +I A+ + +I L+ L + ++A+IG++LF GK C D + EE C E + G W + ++DN A +LT+F T EGW +LY D+ I P V YF+ +II+ +FF++N+ +G + F E E A + K
Sbjct: 103 ARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAIYIPFPEDDSNST-NHNLEKVEYAFLIIFTVETFLKIIASGLLLHPNASVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSG-----------WVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDA-------IGWEWPWV---YFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 424 HSP 3 Score: 104.76 bits (260), Expect = 3.322e-21 Identity = 71/255 (27.84%), Postives = 129/255 (50.59%), Query Frame = 0
Query: 608 AFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEI---NPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
A V S F + + ++ +NTL+++ + Y QP+ T D+ N + FT E + K+ + + YF +N FD +V G + + + E+ +P G ++ FR R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG + T R + F FPQALL +F+ TGE W +M G++ G P+ G YFI F+ ++++L LF+A+ +DN
Sbjct: 577 AAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLG----VSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKR-STFDNFPQALLTVFQILTGEDWNAVMYD-----GIMA------YGGPSSSGMIVCI-YFIILFICGNYILLKLFLAIAVDNL 814 HSP 4 Score: 73.1738 bits (178), Expect = 1.608e-11 Identity = 72/329 (21.88%), Postives = 129/329 (39.21%), Query Frame = 0
Query: 589 NAKPVRRYIP---KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPE----GYTAFLDVLNVIFTVFFTLEFIFKL---GAFRFKNY-FGDPWNAFDFIIVLGSLIDIAMAEINP---GGNMIS-------INFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-----------------------------GKIAPQEGTNIHRN--------NNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
N++P R NP++ + V F+ I I N ++LA+ + PE L+ + F + FT+E K+ G N + WN DF+IV+ L + + ++ GGN S + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F G+ GT NF F A+L +F+ T E W +++ G ++ + YF+S ++ SF +LNL + V+ F
Sbjct: 99 NSRPARALFCLSLNNPIRRACISIVDWKPFDIFILLAIFANCVALAI-YIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIASGLLLHPNASVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVND-----------------AIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEF 409
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|116241275|sp|Q01668.2|CAC1D_HUMAN (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1D; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 2; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.3) HSP 1 Score: 1191.79 bits (3082), Expect = 0.000e+0 Identity = 650/1139 (57.07%), Postives = 811/1139 (71.20%), Query Frame = 0
Query: 14 IGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKD-----------EDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPG-----------------GNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSE-DAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRR 1123
+ R+W +LSNLV SL NS++SIASLLLLLFLF+ IF+LLGMQ+FG +FN+D Q R FDNF Q+L+TVFQILTGEDWN VMYDGI A+GG S G++ +YFIILFICGNYILLNVFLAIAVDNLADA++L +K+ + E+ +++ + + + K+ +D Y +D D+ D Y P + E + D E G R RR+ + PIPE SAFF+ N R+ CH + NH F N++LV IM+SSA LAAEDP+ S+S RN IL YFDY FT IFTVE+ LK+ +G LH GAFCR+ FNLLD+LVV VSL+SF S AISVVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNI++VT LLQFMFA IGVQLFKGKF+ CTD +K E C+G FI YKDGD++ P+V ERIW + F++DNV ++ LF VSTFEGWP +LY +IDSN E+ GPI+ R ++IF+ IYIII+AFFM+NIFVGFVIVTFQ +GE YKNCELDKNQR C+E+AL A+P+RRYIPKNP QYK W V S FEY +F +I++NTL LAM+ Y Q + + +D+LN++FT FT+E + K+ AF+ K YF D WN FD +IV+GS+ID+A++E +P N ISI FFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGK+A ++ I+RNNNFQTFPQA+L+LFR ATGEAWQEIML+C PG LCD +S+ + G+ CG++FA YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF R+WSEYDP+AKGRIKH+DVVTLLR+I PPLGFGKLCPHRVACK RLV+MNMPLNSDG+V FNATLFALVRT+L IKTEGN+++ANEELR I K+W++T +KLLDQ+ P AG +DEVTVGKFYATFLIQDYFR+FKK+KE + ++P + ++ LQAGLRTLH+ GPE++RAIS +L + EE+ + +RN GN V+ + + R
Sbjct: 629 VTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDE-TQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDK----------DPYP-----------------------PCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACL--PGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK--------------------------------RLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAG-DDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYP-AKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDR 1697 HSP 2 Score: 130.568 bits (327), Expect = 5.425e-29 Identity = 108/344 (31.40%), Postives = 157/344 (45.64%), Query Frame = 0
Query: 271 ASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL---NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF------------SSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF-MC--TDRSKMTEE------------TCQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A A F +N R C I F +L+ I + LA P +SNS N L +Y F IFTVE LK+IAYG +LHP A+ R+G+NLLD ++V V L S + SSG VK LR RVLRPLR ++ L+ V+ +I A+ + +I L+ L + ++A+IG++LF GK C D + EE C E + G W + ++DN A +LT+F T EGW +LY D+ GF + YF+ ++I +FF++N+ +G + F E E A + K
Sbjct: 103 ARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNST-NHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSG-----------WVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDA--------MGFE--LPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK 424 HSP 3 Score: 106.686 bits (265), Expect = 1.022e-21 Identity = 72/255 (28.24%), Postives = 130/255 (50.98%), Query Frame = 0
Query: 608 AFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEI---NPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
A V S F + + ++ +NTL+++ + Y QP+ T D+ N + FT E + K+ + + YF +N FD +V G + + + E+ +P G I+ FR R++R+ K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG + T R + F FPQALL +F+ TGE W +M G++ G P+ G YFI F+ ++++LN+F+A+ +DN
Sbjct: 518 AAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLG----ISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKR-STFDNFPQALLTVFQILTGEDWNAVMYD-----GIMA------YGGPSSSGMIVCI-YFIILFICGNYILLNVFLAIAVDNL 755 HSP 4 Score: 76.2554 bits (186), Expect = 1.775e-12 Identity = 72/329 (21.88%), Postives = 128/329 (38.91%), Query Frame = 0
Query: 589 NAKPVRRYIP---KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPE----GYTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINP---GGNMIS-------INFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-----------------------------GKIAPQEGTNIHRN--------NNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
N++P R NP++ + V F+ I I N ++LA+ + PE L+ + F + FT+E K+ A+ Y + WN DF+IV+ L + + ++ GGN S + R FRV+R ++L+S ++ +L + K+ L +A+L+ + IY ++G+++F G+ GT NF F A+L +F+ T E W +++ G + + YF+S + SF +LNL + V+ F
Sbjct: 99 NSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAI-YIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMND-----------------AMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 409
BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Match: gi|116408|sp|P07293.1|CAC1S_RABIT (RecName: Full=Voltage-dependent L-type calcium channel subunit alpha-1S; AltName: Full=Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; AltName: Full=Dihydropyridine receptor alpha-1S subunit; Short=DHPR; AltName: Full=Voltage-gated calcium channel subunit alpha Cav1.1) HSP 1 Score: 1191.41 bits (3081), Expect = 0.000e+0 Identity = 704/1381 (50.98%), Postives = 886/1381 (64.16%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEV-GRTYGQD--GYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGD-----RMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINP---------------------GGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDA-GDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEK-GPELKRAISGNL---DEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNVSLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDFPSDA------------------------ANIQM-------------RPVKLANGGSQDHSRPDYQYLHPYYNA-----NGDMVAPGGSGSKSVP-------SSPSDRKQ-SMPIVGSAEN----------------------LVGRVLRDQGLGKY-CDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQA 1281
LL I +YW +LSNLV SL NSI+SIASLLLLLFLF+ IFALLGMQ+FG R++++ + R NFDNF Q+LI+VFQ+LTGEDWN VMY+GI A+GG G+L +YFIILF+CGNYILLNVFLAIAVDNLA+A++L +K + EE K + M + PD ++ V + Q G +P+ + + V + + P S +F G DEEDE + R R + +PIPEAS+FF+F N+ R++CH I N ++F N +L+ I++SSA LAAEDP+ + S RN+IL YFD FT++FTVE+ LK+ YG LH G+FCR+ FN+LDLLVVAVSLIS S ISVVKILRVLRVLRPLRAINRAKGLKHVVQCV VA++TIGNIVLVT LLQFMFA IGVQLFKGKFF C D SKMTEE C+G + YKDGD + R W N FH+DNV +++LF VSTFEGWP +LY +IDSNEED GP++ R +AIF+ IYII+IAFFM+NIFVGFVIVTFQ +GE+ YKNCELDKNQR C+++AL A+P+R YIPKNP QY++W VTS FEY +FA+I++NT+ L M+ Y Q E D+LNV FT+ FTLE I KL AF+ + YFGDPWN FDF+IV+GS+ID+ ++EI+ IS FFRLFRVMRL+KLLS+ EG+RTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA +GT I+RNNNFQTFPQA+L+LFR ATGEAWQEI+L+C G LCD +S+ A G+ CGT+FAY YFISF+++C+FLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF +W+EYDP+AKGRIKH+DVVTLLR+I PPLGFGK CPHRVACK RLV MNMPLNSDG+V FNATLFALVRT+L IKTEGN ++ANEELR I K+W+RT +KLLDQ+ P G +DEVTVGKFYATFLIQ++FR+F K++E + P ++ +V +QAGLRT+ E+ PE++R ISG+L +E+ AM EE + RR FG V + R P V N +Q A+I M+ F EDFP DA +N QM A G+ HS ++ L P+ NG +V PG +++ P + P +R Q + GS ++ L+ L GL D FV A S+ + +A M PEE + AA LL+A
Sbjct: 532 LLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVR-RSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKA---------EERK--------RRKMSRGLPDKTEEEKSVMAKKLEQKPKGEGIPTTAKLKVDEFESNVNEV-----KDPYPSADFPG----DDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKVRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRAESVRNQILGYFDIAFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFFSCNDLSKMTEEECRGYYYVYKDGDPTQMELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYRAIDSNEEDMGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYHQSEEMNHISDILNVAFTIIFTLEMILKLLAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSY--GKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACK--------------------------------RLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIG-DDEVTVGKFYATFLIQEHFRKFMKRQE-EYYGYRP--KKDTVQIQAGLRTIEEEAAPEIRRTISGDLTAEEELERAMVEAAMEERIFRRTGGLFGQ-VDTFLERTNSLPPVMANQRPLQFAEI----EMEELESPVFL----EDFPQDARTNPLARANTNNANANVAYGNSNHSNNQMFSSVHCEREFPGEAETPAAGRGALSHS---HRALGPHSKPCAGKLNGQLVQPGMPINQAPPAPCQQPSTDPPERGQRRTSLTGSLQDEAPQRRSSEGSTPRRPAPATALLIQEALVRGGLDTLAADAGFVTATSQALADACQMEPEEVEVAATELLKA 1835 HSP 2 Score: 216.468 bits (550), Expect = 1.863e-55 Identity = 182/698 (26.07%), Postives = 317/698 (45.42%), Query Frame = 0
Query: 247 EDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL--NSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVF--------------SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGK-----FFMCTDRSKMTEET-------------CQGQFIEYKDGDLNYPIVEERIW---DRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESA---------YKNCELDKNQRNCIEFALNAKP-----------------------------------VRRYIPKNPV-QYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNM--ISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
+DEG +R+ + P+ +P P A F N R C I F I+L+ I + LA P+ + N+ N L +YFF T+F++E +K+IAYGF+ H A+ RSG+N+LD ++V + + + + S GA VK LR RVLRPLR ++ L+ V+ + A+ + +I L+ L + ++A+IG++LFKGK +++ TD E C E + G W + H+DN +LT++ T EGW +LY D+ + I YF+ +I++ +FF++N+ +G + F E E A + +L+++ R + + + +R + N V ++K V S +F + + ++ +NTLS+A + + QP T D+ N + FT+E + K+ + YF +N FD +V ++++ + E G M + I+ R R++RL K+ + L+ + S +++ + +L+ L I+ ++GMQ+FG E T + R +NF FPQAL+ +F+ TGE W +M + G++ G P+ G YFI FV ++++LN+F+A+ +DN
Sbjct: 7 QDEG--LRKKQPKKPLPEVLPRP-PRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLTVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNVIQSNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYYIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGG-----------WPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWI----------YFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVEDLREGKLSLEEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSRVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDIANRVLLSLFTIEMLLKMYGLGLRQYFMSIFNRFDCFVVCSGILELLLVE---SGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRR-SNFDNFPQALISVFQVLTGEDWNSVMYN-----GIMA------YGGPSYPGV-LVCIYFIILFVCGNYILLNVFLAIAVDNL 664
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1317990188|ref|XP_012282194.2| (muscle calcium channel subunit alpha-1 isoform X4 [Orussus abietinus]) HSP 1 Score: 1562.74 bits (4045), Expect = 0.000e+0 Identity = 850/1394 (60.98%), Postives = 992/1394 (71.16%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNE-------------------------FDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPG-VLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV----SLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDFP------------------SDAA---------------NIQMRPV----------------KLANG-GSQDHSRPDYQYLHPYYNANGDMVAPGGSGSKSVPSSPSDRKQSMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGS-LEFADQPGWDE--DDETVPLRAPPSRFMIPEHQP 1318
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN L KPR NFD+F+QSL+TVFQILTGEDWN VMY+GI+A+GGV S G+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L EK+ E+G GK+ G P D + G +QDP++ + A S E I D + + R RRM + PIP SAFFLF N+FR+ CH++CNHSYFGN++L CIM+SSAMLAAEDPLN+ S RN ILNYFDYFFTT+FTVE+CLK+I+YGFI+H GAFCRS FN LDLLVV VSL+S FSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQF+FAVIGVQLFKGKFF C+D SKMT E C+G ++ Y++G++N P + ER W RN FH+D+VAK +LTLF VSTFEGWP +LYVSIDSN ED+GPI+ FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ VQYK+W FVTS FEYTIF +I++NT++LAMKFYRQPE YT LDVLN+IFT F LEF+FKL AFRFKNYFGD WN FDFIIVLGS IDI +E++PG ++ISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA T IHRNNNFQTFPQA+LVLFRSATGE WQEIML C PG V CD KS+D + AGCG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G++DEVTVGKFYATFLIQDYFRRFKK+KE + + E +VTLQAGLRTLHE GPELKRAISGNL+E+++ + E MHRRN FG+V SS+ R G N SL VNS +K SPTNS+D+ S ++ D SD+A NI +RP+ K+ +G GS ++ P+ +Y+ +NG + A S S+P SP+DRK + ++GSAE+LVGRVL +QGLGKYCDPDFVR SREMQEA+DMT EE D+AAH+LL ER+G L + Q G D+ + + P S + E QP
Sbjct: 564 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNVSDLQDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYEGIRAYGGVSSPGVLACVYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEA----EEG----AGKNKSNSGSP--------PPDEVSGEGGDDGGEGTGGEDEGGGTDLEQDPNETMEDYEAALDTEASEKGEDMNSHAKVRLNIESDEEDLEGEDEDGSAIHDDGMDGEVSARPRRMSEFNMATKKQPIPAGSAFFLFSQTNRFRVFCHWLCNHSYFGNVILACIMISSAMLAAEDPLNAGSYRNMILNYFDYFFTTVFTVEICLKMISYGFIIHEGAFCRSAFNNLDLLVVCVSLVSMFFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTSLLQFVFAVIGVQLFKGKFFYCSDGSKMTNEECRGTYLVYEEGNINRPTMRERTWARNRFHFDDVAKAMLTLFTVSTFEGWPTLLYVSIDSNAEDYGPIYNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRVQYKVWWFVTSQPFEYTIFTLIMINTVTLAMKFYRQPEFYTEALDVLNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEVSPGSSLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQIFGKIALDTETAIHRNNNFQTFPQAVLVLFRSATGEGWQEIMLDCSHRPGEVKCDPKSDDHENHAGCGSDMAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGVDDEVTVGKFYATFLIQDYFRRFKKRKE-QEMKEGDKDCHNTVTLQAGLRTLHEAGPELKRAISGNLEELLD-----DNPEPMHRRNHSLFGSVWSSM---RRGHHNFHRARSLKVNSTT-SKASPTNSIDFLPYASLQRGGGIDGSNQITARSHQVVPNVAGGLSDSAMNQMGIDPRLSGGEENIPLRPLAVFSNVPQYSNYQAAHKVLDGPGSGNYLHPNSEYVSWAGESNGSVGAE--RLSHSLPGSPADRKPNFEVIGSAESLVGRVLVEQGLGKYCDPDFVRYTSREMQEALDMTREEMDRAAHQLLLQERRGQPLTYQLQQGMDQQWNPAYQQNQQPVSYHPVQEQQP 1897 HSP 2 Score: 135.191 bits (339), Expect = 5.479e-28 Identity = 98/323 (30.34%), Postives = 156/323 (48.30%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP---LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPI--------VEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P L+SN + N L +Y F IFTVE +K+IAYGF+ HPGA+ R+G+NLLD +V + ++S V + VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK ++ T Q + G +P + W+ + ++DN +LT+F T EGW +LY D+ I YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 69 ALFCLPLKNPVRKMCIGVVEWKPFEWLILMTIFANCVALAVYTPYPFLDSN-QTNHYLEKIEYIFLVIFTVECVMKIIAYGFVAHPGAYLRNGWNLLDFTIVVIGMVSTVLTILMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKM----HKTCWNNVTNQMMDDPHPCGSGGFPCESLGPEYYCSHQFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLYDIEDAMGSSWQWI----------YFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 376 HSP 3 Score: 114.775 bits (286), Expect = 8.380e-22 Identity = 74/259 (28.57%), Postives = 130/259 (50.19%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKK------DPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S +F + I ++ +NT+ LA + Y+QP+ F +V NV F F++E + K+ + F+ YF +N FD +V+GS+ ++ + N P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +NF +F Q+LL +F+ TGE W +M + PGVL A YFI F+ ++++LN+F+A+ +DN
Sbjct: 461 VKSQVFYWLIIVLVFLNTVVLATEHYKQPKWLDQFQEVTNVFFIALFSMEMMLKMYSLGFQGYFVSLFNRFDCFVVIGSISEMILTNTNIMPPLG----VSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNVSDLQDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYEGIRAYGGVSSPGVL------------------ACVYFIILFICGNYILLNVFLAIAVDNL 697 HSP 4 Score: 97.8265 bits (242), Expect = 1.244e-16 Identity = 123/586 (20.99%), Postives = 239/586 (40.78%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNY------------DPLAQKPRG-------------------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQY-DEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYF----DYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + DP G NFDNF +++TVFQ +T E W V+YD A G +YFI + I G + ++N+ L + + K+ + GD K+ Q DD Y D + T +D P DE QQD +K+ +++ +G +E R +R + + + + R C F +++V + +++ +LA E + ++ K L+ F + FF +F++E+ LK+ + GF G F S FN D VV S+ + ++ I V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF + DL D+ ++D+ + LLT+F + T E W ++Y I + +G + + +++ I I + ++N+F+ +
Sbjct: 208 SLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCWNNVTNQMMDDPHPCGSGGFPCESLGPEYYCSHQFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLYDIEDAMGSS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS---------KEREKAKARGDFH------------KLREKQQIEDDLKGYLDWI--TQAED--IEPEADEPQQQDGK-------SKQQNEMESTDQLEGDDEGVQQESFWKRKKRDLDRA-------------------NRRMRRACRKAVKSQVFYWLIIVLVFLNTVVLATE-----HYKQPKWLDQFQEVTNVFFIALFSMEMMLKMYSLGF---QGYFV-SLFNRFDCFVVIGSISEMILTNTNIMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNV---------------------SDLQ---------DKPRSNFDSFWQSLLTVFQILTGEDWNAVMYEGIRA----YGGVSSPGVLACVYFIILFICGNYILLNVFLAIAV 694 HSP 5 Score: 87.0409 bits (214), Expect = 2.918e-13 Identity = 80/352 (22.73%), Postives = 143/352 (40.62%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKF---YRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRN------------------------------------NNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISP 906
KNPV+ V FE+ I I N ++LA+ + +L+ + IF V FT+E + K+ A+ F Y + WN DF IV+ ++ + + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK+ N+ NF F A+L +F+ T E W +++ EDA G+ + + YFIS ++ +F ++NL + V+ F G H +R + + D KG ++D +T I P
Sbjct: 76 KNPVRKMCIGVVEWKPFEWLILMTIFANCVALAVYTPYPFLDSNQTNHYLEKIEYIFLVIFTVECVMKIIAYGFVAHPGAYLRNGWNLLDFTIVVIGMVSTVLTILMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCWNNVTNQMMDDPHPCGSGGFPCESLGPEYYCSHQFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLYDI------------EDA-----MGSSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEDDLKG---YLDWITQAEDIEP 402
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1101361135|ref|XP_018912860.1| (PREDICTED: muscle calcium channel subunit alpha-1 isoform X6 [Bemisia tabaci]) HSP 1 Score: 1549.26 bits (4010), Expect = 0.000e+0 Identity = 833/1348 (61.80%), Postives = 988/1348 (73.29%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADAL--------------EDEEKDEDDPPEDG-DDDEEGKDGGEDGETK-IPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRM---RRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPG-VLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPN--VSLNVNSVQHA------------KISPTNSMDYAAQNSFRK------------------------MPDEDFPS-DAANIQMRPVKLANGGSQDHSRP--DYQYLHPY-YNANGDMVAPGGSGSKSVPSSPSDRKQSMP-IVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG-SLEFAD 1291
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN+DP KPR NFD+F+QSL+TVFQILTGEDWN VMYDGI+A+GGV SIGIL+ +Y+IILFICGNYILLNVFLAIAVDNLADA++L + P EDG +D+ G+ E+G K MD ++ DE R G++ ED+ S+ + +G DYDEE +G + R+M S T PIP ++FF+ DN+FR+ CH+ CNHS F N++L CI++SSA+LAAEDPL S + RNK+L YFDYFFTT+FT E+C K+I YGFI H GAFCRS FNLLDLLVV VSL+S SSGA+S +KILRV RVLRPLRAINRAKGLK+VV+CVIVA+KTIGNI+LVT LLQFMFAVIGVQLFKGKFF C+D SKMTE CQG ++ + GD++ P VEER+W RN FH+D+VAK +LTLF VSTFEGWPG+LYVSIDSNEED GPIH +RPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ QYK+W FVTS FEYTIF +I++NT++LAMKFY+QP+GYT LDV+N+ FT F LEF+FKL AFRFKNYFGD WN FDFIIVLGS IDI ++NPG ++ISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA T IHRNNNFQTFPQA+LVLFRSATGEAWQ+IML C PG V CD +S+DA + GCG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+ NEELR I+K+W+RT +LLDQ+ P G++DEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLH+ GPELKRAISGNL+E+I+ + E MHRRN FG+V SS++ R G+ N SL +N+ Q K+SPTNS+D NS +K M +D D A I ++P+ ++N G +P QYLHPY Y + D ++S+P SP+DR+ + P +VGSAE+LVGRVL +QGLGK+ DPDFVR SRE+QEA++MT EE D+AAH+L+ AERQG S F D
Sbjct: 539 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDPSQFKPRSNFDSFWQSLLTVFQILTGEDWNTVMYDGIRAYGGVASIGILACIYYIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEEDDEAEKQQKYASGSPGPKEDGMSNDDMGQTDDENGSLKGSEMDN---QSQNETDENERYSGEEEMEEEEEDD------------------NQGSVVEATNPVGEDYDEEPKGRKTALPRKMSDTSQAQETKPIPAGTSFFIMSQDNRFRVFCHWFCNHSTFSNVILACILISSALLAAEDPLTSENPRNKVLVYFDYFFTTVFTTEICFKMITYGFIFHDGAFCRSAFNLLDLLVVLVSLVSVFSSSGAVSFIKILRVFRVLRPLRAINRAKGLKYVVKCVIVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFSCSDGSKMTEAECQGTYLVFDHGDIDRPTVEERVWKRNRFHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSNEEDVGPIHNYRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRFQYKIWWFVTSQPFEYTIFVLIMINTITLAMKFYKQPQGYTDALDVMNLFFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIIYTDLNPGSSIISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNSETAIHRNNNFQTFPQAVLVLFRSATGEAWQDIMLDCSSRPGEVRCDPRSDDANNLNGCGSDIAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDCNEELRAVIMKIWKRTSRQLLDQVVPPPGVDDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGDKESHNTVTLQAGLRTLHDAGPELKRAISGNLEELID-----DNPEPMHRRNHSLFGSVWSSMR-RHQGSFNRARSLRLNTSQIKMNHLDVPGRNLRKVSPTNSIDSLPFNSMQKVVTGGVNHITNFTRNTFSPQITNAMSMQDMKKDDEAMIPLQPLTISNSGPPSREKPVIGTQYLHPYAYGDDPDGTKAAERLARSLPGSPADRRPNFPEVVGSAESLVGRVLAEQGLGKFADPDFVRNTSRELQEALNMTEEEMDRAAHQLMVAERQGRSFSFQD 1826 HSP 2 Score: 133.265 bits (334), Expect = 2.361e-27 Identity = 107/325 (32.92%), Postives = 159/325 (48.92%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL-NSNSER-NKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFF----------MCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERI-WDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P NS+S R N L +Y F IFTVE +K++AYGF+ HPGA+ R+G+NLLD +V + +IS S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ + + ++A+IG++LF GK M D EE Q F Y G I E R+ W+ F ++DN +LT+F T EGW +LY DS I YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 41 ALFCLTLKNPLRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNRTNACLENIEYVFLVIFTVECVMKIVAYGFVAHPGAYLRNGWNLLDFTIVVIGMISTTLSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVIFVIIIYAIIGLELFSGKLHKTCFNNITGEMMDDPHPCGEEGFQCSF--YNKG--TEEIWECRLDWEGPHFGITNFDNFGLAMLTVFQCITLEGWTDVLYDIEDSLGNTWQWI----------YFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 351 HSP 3 Score: 112.849 bits (281), Expect = 3.972e-21 Identity = 73/253 (28.85%), Postives = 128/253 (50.59%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT LA + Y QPE F +V N+ F FT+E + K+ + F+ YF +N FD +V+GS+ ++ + N P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG + + +NF +F Q+LL +F+ TGE W +M + G A G A Y+I F+ ++++LN+F+A+ +DN
Sbjct: 436 VKSQAFYWLIIILVFLNTGVLATEHYHQPEWLDEFQEVTNMFFIALFTMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSISEMVLTHTNIMPPLG----VSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDPSQFKPRSNFDSFWQSLLTVFQILTGEDWNTVMY-----------DGIRAYGGVASIGI-LACIYYIILFICGNYILLNVFLAIAVDNL 672 HSP 4 Score: 71.633 bits (174), Expect = 1.295e-8 Identity = 82/356 (23.03%), Postives = 144/356 (40.45%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEG----YTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRN---------------------------------------NNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISP 906
KNP++ V FEY I I N ++LA+ + P A L+ + +F V FT+E + K+ A+ F Y + WN DF IV+ +I ++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK+ NI NF F A+L +F+ T E W +++ ED+ G + + YFIS ++ +F ++NL + V+ F G H +R + + D KG ++D +T I P
Sbjct: 48 KNPLRKLCIDVVEWKPFEYLILLTIFANCVALAV-YTPYPNSDSNRTNACLENIEYVFLVIFTVECVMKIVAYGFVAHPGAYLRNGWNLLDFTIVVIGMISTTLSNLMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVIFVIIIYAIIGLELFSGKLHKTCFNNITGEMMDDPHPCGEEGFQCSFYNKGTEEIWECRLDWEGPHFGITNFDNFGLAMLTVFQCITLEGWTDVLYDI------------EDS-----LGNTWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEEDLKG---YLDWITQAEDIEP 377 HSP 5 Score: 68.1662 bits (165), Expect = 1.484e-7 Identity = 61/265 (23.02%), Postives = 124/265 (46.79%), Query Frame = 0
Query: 299 IVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
++++ + +++ +LA E + ++ + FF +FT+E+ LK+ + GF G F S FN D VV S+ V + I V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF D S+ + ++D+ + LLT+F + T E W ++Y I + +G + + I+Y I I + ++N+F+ +
Sbjct: 444 LIIILVFLNTGVLATEH-YHQPEWLDEFQEVTNMFFIALFTMEMLLKMYSLGF---QGYFV-SLFNRFDCFVVIGSISEMVLTHTNIMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNF--DPSQFKPRS----------------------------NFDSFWQSLLTVFQILTGEDWNTVMYDGIRA----YGGVASIGILACIYYIILFICGNYILLNVFLAIAV 669
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1330916720|gb|PNF34464.1| (Voltage-dependent calcium channel type D subunit alpha-1 [Cryptotermes secundus]) HSP 1 Score: 1548.87 bits (4009), Expect = 0.000e+0 Identity = 832/1360 (61.18%), Postives = 972/1360 (71.47%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALE------------DEEKDEDDPPEDGD--------------DDEEGKDGGEDG-ETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV-SLNVNSVQHA------------KISPTNSMDYAAQNSFRKMPDEDFP--------------SDAANIQ-------------MRPVKLA--NGGSQDHSRPDY--------QYLHPYYNANGDMVAPGGSG----SKSVPSSPSDRKQSMP-IVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG 1285
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +F+++ + KPR NFD+F+QSL+TVFQILTGEDWN VMY GIQA+GGV SIG+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L ++ P D DD E + E G +TK+P++ +DY YD+ +T QD + D V +R++ RP RM S PIP+ SAFF+F N+ R+ CH++CNHSYFGN++L CIM+SSAMLAAEDPL S++ RN+IL FD FFT++FTVE+CLK+I+YGF+LH GAFCRS FNLLDLLVV VSLI+ AISVVKILRVL+VLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQLFKGKFF C+D SKMTE C+G ++ + DGD+N P V+ER W RN H+D+VAK +LTLF VSTFEGWPG+LYVSIDS+EED GPIH RPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKF+ QP Y FLDVLN+IFT F +EF+FKL AFRFKNYFGD WN FDF+IVLGS IDI +E+NPG MISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA GT IHRNNNFQTFPQA+LVLFRSATGEAWQEIML C V CD KS D D CG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G+EDEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLH+ GPELKRAISGNL+E+++ + E MHRRN FG+V SS++ G SL VNS Q K+SPTNS+DY NS + E + A ++Q +RP+ +A NG + P + Q+LHP + + + G G S S+P SP+DR+ + P +VGSAE+LVGRVL +QGLGKYCD DFVR SREMQEA+DMT EE D+AAHRLL ERQG
Sbjct: 518 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQAYGGVSSIGVLACVYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEEAEKQQKSHSGSPTRDEGQGGEENGEGQEEGQDDAENMEKEEAGSDTKVPLEHGDSEDY-PYDDEEQTDNQD-----------EDDDGIEVKRRMSARPH-------------------------RMSEISLKTTIRPIPQGSAFFIFSSTNRIRVFCHWLCNHSYFGNVILACIMISSAMLAAEDPLQSDTPRNEILGKFDIFFTSVFTVEICLKMISYGFVLHNGAFCRSAFNLLDLLVVFVSLIAMFIRGSAISVVKILRVLKVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCSDGSKMTEAECRGTYLIFDDGDINQPQVDERKWTRNRLHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSHEEDEGPIHNSRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFILIMLNTITLAMKFHNQPPAYEKFLDVLNIIFTAVFAMEFVFKLAAFRFKNYFGDAWNVFDFVIVLGSFIDIIYSEVNPGSGMISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALNPGTAIHRNNNFQTFPQAVLVLFRSATGEAWQEIMLDCSAREEVKCDPKS-DETDKLICGSDMAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGVEDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGDKESHNTVTLQAGLRTLHDAGPELKRAISGNLEELMD-----DNPEPMHRRNHSLFGSVWSSMRRGHAGFHRARSLKVNSTQIKMNHLDVPGRNLRKVSPTNSIDYLPYNSIQHAVTEGMNHITSMTKSTLMPHIASATSMQETGGGLQEEEAIPLRPLTVAVSNGDPEKTFSPYHVIDQHGGGQFLHPNHYVSRAGESNGSLGTERLSHSLPGSPADRRPNFPEVVGSAESLVGRVLVEQGLGKYCDADFVRYTSREMQEALDMTQEEMDRAAHRLLLQERQG 1801 HSP 2 Score: 131.339 bits (329), Expect = 8.763e-27 Identity = 100/327 (30.58%), Postives = 159/327 (48.62%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL-NSNS-ERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETC----QGQFIE--YKDGDLNYP--IVEERIWDRNAF--------HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P NS+S N L + F IFTVE +K+IAYG + HPGA+ R+G+NLLD +V + L+S S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK ETC G+ ++ + G+ + I+ + + RN + ++DN +LT+F T EGW +LY D+ I YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 23 ALFCLPLKNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKL----------HETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNSWQWI----------YFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 329 HSP 3 Score: 112.464 bits (280), Expect = 4.661e-21 Identity = 73/252 (28.97%), Postives = 130/252 (51.59%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAM--AEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT+ LA + YRQP+ F D N+ F F++E + K+ + F+ YF +N FD +V+GS+ +I + E+ P + ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +NF +F Q+LL +F+ TGE W +M G+ + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 415 VKSQAFYWLIIMLVFLNTVVLATEHYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEIILTHTEVMPP---LGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYV-----GI------QAYGGVSSIGV-LACVYFIILFICGNYILLNVFLAIAVDNL 651 HSP 4 Score: 93.9745 bits (232), Expect = 2.261e-15 Identity = 118/584 (20.21%), Postives = 235/584 (40.24%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFN---YDPLAQK------PRG---------------------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYF----DYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + YD + + P G NFDNF +++TVFQ +T E W V+Y A G +YFI + I G + ++N+ L + + +E+++ D E + ED Y D Q +++ ED QD + R+ E + + ++ + DR R + R C F ++++ + +++ +LA E + + K L++F + FF +F++E+ LK+ + GF G F S FN D VV S+ + + + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF D N I D+ ++D+ + LLT+F + T E W ++YV I + +G + + +++ I I + ++N+F+ +
Sbjct: 162 SLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS-------KEREKAKARGDFHKLREKQQIEEDLRG-------YLDWITQAEDI---------EPEGEDRSRNQDGKPKIANETETADRAEGEEGEVEQESWWRKKKKDFDRNNR--------------------------RMRRACRKAVKSQAFYWLIIMLVFLNTVVLATE-----HYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGF---QGYFV-SLFNRFDCFVVIGSIGEIILTHTEVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF------------------------DFN------SITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQA----YGGVSSIGVLACVYFIILFICGNYILLNVFLAIAV 648 HSP 5 Score: 84.3445 bits (207), Expect = 1.960e-12 Identity = 82/351 (23.36%), Postives = 147/351 (41.88%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKF-YRQPEGYTA--FLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIH--------------------------RNN---------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISP 906
KNP++ V FEY I I N ++LA+ Y + + +L+ + +F FT+E + K+ A+ Y + WN DF IV+ L+ A++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK+ N+ RNN NF F A+L +F+ T E W +++ EDA G + + YFIS ++ +F ++NL + V+ F G H +R + + D +G ++D +T I P
Sbjct: 30 KNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYV------------EDA-----MGNSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEEDLRG---YLDWITQAEDIEP 355
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1330916721|gb|PNF34465.1| (Voltage-dependent calcium channel type D subunit alpha-1 [Cryptotermes secundus]) HSP 1 Score: 1544.25 bits (3997), Expect = 0.000e+0 Identity = 832/1362 (61.09%), Postives = 971/1362 (71.29%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALE------------DEEKDEDDPPEDGD--------------DDEEGKDGGEDG-ETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEG--DRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV-SLNVNSVQHA------------KISPTNSMDYAAQNSFRKMPDEDFP--------------SDAANIQ-------------MRPVKLA--NGGSQDHSRPDY--------QYLHPYYNANGDMVAPGGSG----SKSVPSSPSDRKQSMP-IVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG 1285
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +F+++ + KPR NFD+F+QSL+TVFQILTGEDWN VMY GIQA+GGV SIG+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L ++ P D DD E + E G +TK+P++ +DY P +DE +Q +Q D DE D+G R RR+ + T PIP AS+FF+F N+ R+ CH++CNHSYFGN++L CIM+SSAMLAAEDPL S++ RN+IL FD FFT++FTVE+CLK+I+YGF+LH GAFCRS FNLLDLLVV VSLI+ AISVVKILRVL+VLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQLFKGKFF C+D SKMTE C+G ++ + DGD+N P V+ER W RN H+D+VAK +LTLF VSTFEGWPG+LYVSIDS+EED GPIH RPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKF+ QP Y FLDVLN+IFT F +EF+FKL AFRFKNYFGD WN FDF+IVLGS IDI +E PG MISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA GT IHRNNNFQTFPQA+LVLFRSATGEAWQEIML C V CD KS D D CG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G+EDEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLH+ GPELKRAISGNL+E+++ + E MHRRN FG+V SS++ G SL VNS Q K+SPTNS+DY NS + E + A ++Q +RP+ +A NG + P + Q+LHP + + + G G S S+P SP+DR+ + P +VGSAE+LVGRVL +QGLGKYCD DFVR SREMQEA+DMT EE D+AAHRLL ERQG
Sbjct: 260 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQAYGGVSSIGVLACVYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEEAEKQQKSHSGSPTRDEGQGGEENGEGQEEGQDDAENMEKEEAGSDTKVPLEHGDSEDY-----------------PYDDE--EQTDNQ------------------------DMDELDDGITARPRRLSEFNIATKTQPIPPASSFFIFSKSNRIRVFCHWLCNHSYFGNVILACIMISSAMLAAEDPLQSDTPRNEILGKFDIFFTSVFTVEICLKMISYGFVLHNGAFCRSAFNLLDLLVVFVSLIAMFIRGSAISVVKILRVLKVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCSDGSKMTEAECRGTYLIFDDGDINQPQVDERKWTRNRLHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSHEEDEGPIHNSRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFILIMLNTITLAMKFHNQPPAYEKFLDVLNIIFTAVFAMEFVFKLAAFRFKNYFGDAWNVFDFVIVLGSFIDIIYSE--PGSGMISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALNPGTAIHRNNNFQTFPQAVLVLFRSATGEAWQEIMLDCSAREEVKCDPKS-DETDKLICGSDMAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGVEDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGDKESHNTVTLQAGLRTLHDAGPELKRAISGNLEELMD-----DNPEPMHRRNHSLFGSVWSSMRRGHAGFHRARSLKVNSTQIKMNHLDVPGRNLRKVSPTNSIDYLPYNSIQHAVTEGMNHITSMTKSTLMPHIASATSMQETGGGLQEEEAIPLRPLTVAVSNGDPEKTFSPYHVIDQHGGGQFLHPNHYVSRAGESNGSLGTERLSHSLPGSPADRRPNFPEVVGSAESLVGRVLVEQGLGKYCDADFVRYTSREMQEALDMTQEEMDRAAHRLLLQERQG 1537 HSP 2 Score: 112.849 bits (281), Expect = 3.755e-21 Identity = 73/252 (28.97%), Postives = 130/252 (51.59%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAM--AEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT+ LA + YRQP+ F D N+ F F++E + K+ + F+ YF +N FD +V+GS+ +I + E+ P + ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +NF +F Q+LL +F+ TGE W +M G+ + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 157 VKSQAFYWLIIMLVFLNTVVLATEHYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEIILTHTEVMPP---LGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYV-----GI------QAYGGVSSIGV-LACVYFIILFICGNYILLNVFLAIAVDNL 393 HSP 3 Score: 81.2629 bits (199), Expect = 1.429e-11 Identity = 100/487 (20.53%), Postives = 196/487 (40.25%), Query Frame = 0
Query: 81 LITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYF----DYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
++TVFQ +T E W V+Y A G +YFI + I G + ++N+ L + + +E+++ D E + ED Y D Q +++ ED QD + R+ E + + ++ + DR R + R C F ++++ + +++ +LA E + + K L++F + FF +F++E+ LK+ + GF G F S FN D VV S+ + + + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF D N I D+ ++D+ + LLT+F + T E W ++YV I + +G + + +++ I I + ++N+F+ +
Sbjct: 1 MLTVFQCVTLEGWTDVLYYVEDAMGNS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS-------KEREKAKARGDFHKLREKQQIEEDLRG-------YLDWITQAEDI---------EPEGEDRSRNQDGKPKIANETETADRAEGEEGEVEQESWWRKKKKDFDRNNR--------------------------RMRRACRKAVKSQAFYWLIIMLVFLNTVVLATE-----HYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGF---QGYFV-SLFNRFDCFVVIGSIGEIILTHTEVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF------------------------DFN------SITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQA----YGGVSSIGVLACVYFIILFICGNYILLNVFLAIAV 390
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1330916718|gb|PNF34462.1| (Voltage-dependent calcium channel type D subunit alpha-1 [Cryptotermes secundus]) HSP 1 Score: 1540.78 bits (3988), Expect = 0.000e+0 Identity = 831/1360 (61.10%), Postives = 970/1360 (71.32%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALE------------DEEKDEDDPPEDGD--------------DDEEGKDGGEDG-ETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV-SLNVNSVQHA------------KISPTNSMDYAAQNSFRKMPDEDFP--------------SDAANIQ-------------MRPVKLA--NGGSQDHSRPDY--------QYLHPYYNANGDMVAPGGSG----SKSVPSSPSDRKQSMP-IVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG 1285
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +F+++ + KPR NFD+F+QSL+TVFQILTGEDWN VMY GIQA+GGV SIG+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L ++ P D DD E + E G +TK+P++ +DY YD+ +T QD + D V +R++ RP RM S PIP+ SAFF+F N+ R+ CH++CNHSYFGN++L CIM+SSAMLAAEDPL S++ RN+IL FD FFT++FTVE+CLK+I+YGF+LH GAFCRS FNLLDLLVV VSLI+ AISVVKILRVL+VLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQLFKGKFF C+D SKMTE C+G ++ + DGD+N P V+ER W RN H+D+VAK +LTLF VSTFEGWPG+LYVSIDS+EED GPIH RPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKF+ QP Y FLDVLN+IFT F +EF+FKL AFRFKNYFGD WN FDF+IVLGS IDI +E PG MISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA GT IHRNNNFQTFPQA+LVLFRSATGEAWQEIML C V CD KS D D CG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G+EDEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLH+ GPELKRAISGNL+E+++ + E MHRRN FG+V SS++ G SL VNS Q K+SPTNS+DY NS + E + A ++Q +RP+ +A NG + P + Q+LHP + + + G G S S+P SP+DR+ + P +VGSAE+LVGRVL +QGLGKYCD DFVR SREMQEA+DMT EE D+AAHRLL ERQG
Sbjct: 518 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQAYGGVSSIGVLACVYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEEAEKQQKSHSGSPTRDEGQGGEENGEGQEEGQDDAENMEKEEAGSDTKVPLEHGDSEDY-PYDDEEQTDNQD-----------EDDDGIEVKRRMSARPH-------------------------RMSEISLKTTIRPIPQGSAFFIFSSTNRIRVFCHWLCNHSYFGNVILACIMISSAMLAAEDPLQSDTPRNEILGKFDIFFTSVFTVEICLKMISYGFVLHNGAFCRSAFNLLDLLVVFVSLIAMFIRGSAISVVKILRVLKVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKGKFFKCSDGSKMTEAECRGTYLIFDDGDINQPQVDERKWTRNRLHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSHEEDEGPIHNSRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFILIMLNTITLAMKFHNQPPAYEKFLDVLNIIFTAVFAMEFVFKLAAFRFKNYFGDAWNVFDFVIVLGSFIDIIYSE--PGSGMISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALNPGTAIHRNNNFQTFPQAVLVLFRSATGEAWQEIMLDCSAREEVKCDPKS-DETDKLICGSDMAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGVEDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGDKESHNTVTLQAGLRTLHDAGPELKRAISGNLEELMD-----DNPEPMHRRNHSLFGSVWSSMRRGHAGFHRARSLKVNSTQIKMNHLDVPGRNLRKVSPTNSIDYLPYNSIQHAVTEGMNHITSMTKSTLMPHIASATSMQETGGGLQEEEAIPLRPLTVAVSNGDPEKTFSPYHVIDQHGGGQFLHPNHYVSRAGESNGSLGTERLSHSLPGSPADRRPNFPEVVGSAESLVGRVLVEQGLGKYCDADFVRYTSREMQEALDMTQEEMDRAAHRLLLQERQG 1799 HSP 2 Score: 131.339 bits (329), Expect = 8.395e-27 Identity = 100/327 (30.58%), Postives = 159/327 (48.62%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL-NSNS-ERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETC----QGQFIE--YKDGDLNYP--IVEERIWDRNAF--------HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P NS+S N L + F IFTVE +K+IAYG + HPGA+ R+G+NLLD +V + L+S S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK ETC G+ ++ + G+ + I+ + + RN + ++DN +LT+F T EGW +LY D+ I YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 23 ALFCLPLKNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKL----------HETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNSWQWI----------YFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 329 HSP 3 Score: 112.464 bits (280), Expect = 4.659e-21 Identity = 73/252 (28.97%), Postives = 130/252 (51.59%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAM--AEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT+ LA + YRQP+ F D N+ F F++E + K+ + F+ YF +N FD +V+GS+ +I + E+ P + ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +NF +F Q+LL +F+ TGE W +M G+ + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 415 VKSQAFYWLIIMLVFLNTVVLATEHYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEIILTHTEVMPP---LGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYV-----GI------QAYGGVSSIGV-LACVYFIILFICGNYILLNVFLAIAVDNL 651 HSP 4 Score: 93.9745 bits (232), Expect = 2.299e-15 Identity = 118/584 (20.21%), Postives = 235/584 (40.24%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFN---YDPLAQK------PRG---------------------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYF----DYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + YD + + P G NFDNF +++TVFQ +T E W V+Y A G +YFI + I G + ++N+ L + + +E+++ D E + ED Y D Q +++ ED QD + R+ E + + ++ + DR R + R C F ++++ + +++ +LA E + + K L++F + FF +F++E+ LK+ + GF G F S FN D VV S+ + + + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF D N I D+ ++D+ + LLT+F + T E W ++YV I + +G + + +++ I I + ++N+F+ +
Sbjct: 162 SLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS-------KEREKAKARGDFHKLREKQQIEEDLRG-------YLDWITQAEDI---------EPEGEDRSRNQDGKPKIANETETADRAEGEEGEVEQESWWRKKKKDFDRNNR--------------------------RMRRACRKAVKSQAFYWLIIMLVFLNTVVLATE-----HYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGF---QGYFV-SLFNRFDCFVVIGSIGEIILTHTEVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF------------------------DFN------SITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQA----YGGVSSIGVLACVYFIILFICGNYILLNVFLAIAV 648 HSP 5 Score: 84.3445 bits (207), Expect = 1.976e-12 Identity = 82/351 (23.36%), Postives = 147/351 (41.88%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKF-YRQPEGYTA--FLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIH--------------------------RNN---------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISP 906
KNP++ V FEY I I N ++LA+ Y + + +L+ + +F FT+E + K+ A+ Y + WN DF IV+ L+ A++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK+ N+ RNN NF F A+L +F+ T E W +++ EDA G + + YFIS ++ +F ++NL + V+ F G H +R + + D +G ++D +T I P
Sbjct: 30 KNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYV------------EDA-----MGNSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEEDLRG---YLDWITQAEDIEP 355
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1330916717|gb|PNF34461.1| (Voltage-dependent calcium channel type D subunit alpha-1 [Cryptotermes secundus]) HSP 1 Score: 1535.78 bits (3975), Expect = 0.000e+0 Identity = 827/1360 (60.81%), Postives = 970/1360 (71.32%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALE------------DEEKDEDDPPEDGD--------------DDEEGKDGGEDG-ETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV-SLNVNSVQHA------------KISPTNSMDYAAQNSFRKMPDEDFP--------------SDAANIQ-------------MRPVKLA--NGGSQDHSRPDY--------QYLHPYYNANGDMVAPGGSG----SKSVPSSPSDRKQSMP-IVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG 1285
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +F+++ + KPR NFD+F+QSL+TVFQILTGEDWN VMY GIQA+GGV SIG+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L ++ P D DD E + E G +TK+P++ +DY YD+ +T QD + D V +R++ RP RM S PIP+ SAFF+F N+ R+ CH++CNHSYFGN++L CIM+SSAMLAAEDPL S++ RN+IL FD FFT++FTVE+CLK+I+YGF+LH GAFCRS FNLLDLLVV VSLI+ AISVVKILRVL+VLRPLRAINRAKGLK+VV+CVIVA+KTIGNI+LVT LLQFMFAVIGVQLFKGKFF C+D SKMTE C+G ++ + DGD+N P V+ER W RN H+D+VAK +LTLF VSTFEGWPG+LYVSIDS+EED GPIH RPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKF+ QP Y FLDVLN+IFT F +EF+FKL AFRFKNYFGD WN FDF+IVLGS IDI +E PG MISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKIA GT IHRNNNFQTFPQA+LVLFRSATGEAWQEIML C V CD KS D D CG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G+EDEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLH+ GPELKRAISGNL+E+++ + E MHRRN FG+V SS++ G SL VNS Q K+SPTNS+DY NS + E + A ++Q +RP+ +A NG + P + Q+LHP + + + G G S S+P SP+DR+ + P +VGSAE+LVGRVL +QGLGKYCD DFVR SREMQEA+DMT EE D+AAHRLL ERQG
Sbjct: 518 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQAYGGVSSIGVLACVYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEEAEKQQKSHSGSPTRDEGQGGEENGEGQEEGQDDAENMEKEEAGSDTKVPLEHGDSEDY-PYDDEEQTDNQD-----------EDDDGIEVKRRMSARPH-------------------------RMSEISLKTTIRPIPQGSAFFIFSSTNRIRVFCHWLCNHSYFGNVILACIMISSAMLAAEDPLQSDTPRNEILGKFDIFFTSVFTVEICLKMISYGFVLHNGAFCRSAFNLLDLLVVFVSLIAMFIRGSAISVVKILRVLKVLRPLRAINRAKGLKYVVKCVIVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFKCSDGSKMTEAECRGTYLIFDDGDINQPQVDERKWTRNRLHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSHEEDEGPIHNSRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFILIMLNTITLAMKFHNQPPAYEKFLDVLNIIFTAVFAMEFVFKLAAFRFKNYFGDAWNVFDFVIVLGSFIDIIYSE--PGSGMISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALNPGTAIHRNNNFQTFPQAVLVLFRSATGEAWQEIMLDCSAREEVKCDPKS-DETDKLICGSDMAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGVEDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGDKESHNTVTLQAGLRTLHDAGPELKRAISGNLEELMD-----DNPEPMHRRNHSLFGSVWSSMRRGHAGFHRARSLKVNSTQIKMNHLDVPGRNLRKVSPTNSIDYLPYNSIQHAVTEGMNHITSMTKSTLMPHIASATSMQETGGGLQEEEAIPLRPLTVAVSNGDPEKTFSPYHVIDQHGGGQFLHPNHYVSRAGESNGSLGTERLSHSLPGSPADRRPNFPEVVGSAESLVGRVLVEQGLGKYCDADFVRYTSREMQEALDMTQEEMDRAAHRLLLQERQG 1799 HSP 2 Score: 131.339 bits (329), Expect = 7.584e-27 Identity = 100/327 (30.58%), Postives = 159/327 (48.62%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL-NSNS-ERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETC----QGQFIE--YKDGDLNYP--IVEERIWDRNAF--------HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P NS+S N L + F IFTVE +K+IAYG + HPGA+ R+G+NLLD +V + L+S S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK ETC G+ ++ + G+ + I+ + + RN + ++DN +LT+F T EGW +LY D+ I YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 23 ALFCLPLKNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKL----------HETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNSWQWI----------YFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 329 HSP 3 Score: 112.464 bits (280), Expect = 4.581e-21 Identity = 73/252 (28.97%), Postives = 130/252 (51.59%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAM--AEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S F + I ++ +NT+ LA + YRQP+ F D N+ F F++E + K+ + F+ YF +N FD +V+GS+ +I + E+ P + ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG +NF +F Q+LL +F+ TGE W +M G+ + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 415 VKSQAFYWLIIMLVFLNTVVLATEHYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEIILTHTEVMPP---LGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFDFNSITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYV-----GI------QAYGGVSSIGV-LACVYFIILFICGNYILLNVFLAIAVDNL 651 HSP 4 Score: 93.9745 bits (232), Expect = 1.862e-15 Identity = 118/584 (20.21%), Postives = 235/584 (40.24%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFN---YDPLAQK------PRG---------------------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYF----DYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + YD + + P G NFDNF +++TVFQ +T E W V+Y A G +YFI + I G + ++N+ L + + +E+++ D E + ED Y D Q +++ ED QD + R+ E + + ++ + DR R + R C F ++++ + +++ +LA E + + K L++F + FF +F++E+ LK+ + GF G F S FN D VV S+ + + + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF D N I D+ ++D+ + LLT+F + T E W ++YV I + +G + + +++ I I + ++N+F+ +
Sbjct: 162 SLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYVEDAMGNS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS-------KEREKAKARGDFHKLREKQQIEEDLRG-------YLDWITQAEDI---------EPEGEDRSRNQDGKPKIANETETADRAEGEEGEVEQESWWRKKKKDFDRNNR--------------------------RMRRACRKAVKSQAFYWLIIMLVFLNTVVLATE-----HYRQPKWLDHFQDWTNLFFVALFSMEMLLKMYSLGF---QGYFV-SLFNRFDCFVVIGSIGEIILTHTEVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF------------------------DFN------SITDKPRSNFDSFWQSLLTVFQILTGEDWNAVMYVGIQA----YGGVSSIGVLACVYFIILFICGNYILLNVFLAIAV 648 HSP 5 Score: 83.9593 bits (206), Expect = 2.027e-12 Identity = 82/351 (23.36%), Postives = 147/351 (41.88%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKF-YRQPEGYTA--FLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIH--------------------------RNN---------NFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISP 906
KNP++ V FEY I I N ++LA+ Y + + +L+ + +F FT+E + K+ A+ Y + WN DF IV+ L+ A++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK+ N+ RNN NF F A+L +F+ T E W +++ EDA G + + YFIS ++ +F ++NL + V+ F G H +R + + D +G ++D +T I P
Sbjct: 30 KNPIRKLCIDVVEWKPFEYLILLTIFANCVALAVYTPYPNSDSNSTNLYLEKIENVFLAIFTVECVMKIIAYGLVAHPGAYLRNGWNLLDFTIVVIGLLSSALSNLMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHETCYDNVTGEIMDDPHPCGEAGFQCDIIAKNMVCRNNWTGPNNGITNFDNFGLAMLTVFQCVTLEGWTDVLYYV------------EDA-----MGNSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEEDLRG---YLDWITQAEDIEP 355
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|1032744931|ref|XP_008202602.2| (PREDICTED: muscle calcium channel subunit alpha-1 isoform X3 [Nasonia vitripennis]) HSP 1 Score: 1535.01 bits (3973), Expect = 0.000e+0 Identity = 828/1346 (61.52%), Postives = 983/1346 (73.03%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALE-----------DEEKDEDDPPED--GDDDEEGKD--GGEDGETKIPMDQYYPDDYDQYDEV--GRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNM-ISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDP-GVLCD-EKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV--SLNVNSVQHAKISPTNSMDYAAQNSF-RKMPD----------EDFPSDAA---------------------NIQMRPVKLANGGSQDHSRPDYQ---------YLHPYYN-ANGDMVAPGGSG----SKSVPSSPSDRKQSMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQG 1285
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG RFN+ KPR NFD+F+QSL+TVFQILTGEDWNVVMY GI+A+GGV S G+L+ +YFIILFICGNYILLNVFLAIAVDNLADA++L + P +D + ++G D GGED E ++Q + + Y+ G +DG+ D ++ + + + + NE R RRM + +PIP AS+FF+F N+FR+ CH++CNHSYFGN++LVCIM+SSAMLAAEDP++ NS+RNKIL YFDYFFTT+FT+E+CLK I+YGFILH GAFCRS FNLLDLLVV VS++S SSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQF+FAV+GVQLFKGKFFMC DRSK+T E CQG +I+Y+DG++N P+ EER W+RN FH+D+VAK +LTLF VSTFEGWPG+LYVSIDSN+ED GPI+ FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKFY+QP+ YT LD LN++FT F LEFIFKL AFRFKNYFGD WN FDFIIVLGS IDI +E+ G + ISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQ+FGKI + T IHRNN+FQ FPQA+LVLFRSATGEAWQEIM++C +P VLC + P CG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNIDEAN +LR I K+W+RT KLLDQ+ P G++DEVTVGKFYATFLIQDYFRRFKKKKE + D +VTLQAGLRTLHE GPELKRAISGNL+E+++ + E MHRRN FG+V SS++ + N SL +NS +K SPTNS+D+ S R PD + P+ A +I +RP+ + G + RP ++ YLHP + A+ + G G S S+P SP+DRK + ++GSAE+LVGRVL +QGLGKYCDP+FVR SREMQEA++MT EE D+AAH+LL ER+G
Sbjct: 590 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGRFNFSAEDDKPRHNFDSFWQSLLTVFQILTGEDWNVVMYTGIRAYGGVASSGVLACIYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEEAEKNKSRSGSPGQDELSGEQDDGADVTGGEDEEGNTDLEQDPNETMEDYEAALDGSDKDEDGHGKVRLDLESDEEDLEGDEEDDHTEIQDEDIENEVSA------------RPRRMSEFNMGTKIVPIPPASSFFVFSQTNRFRVFCHWVCNHSYFGNVILVCIMISSAMLAAEDPIDPNSQRNKILAYFDYFFTTVFTIEICLKTISYGFILHDGAFCRSSFNLLDLLVVCVSIVSTQLSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTSLLQFVFAVVGVQLFKGKFFMCNDRSKITSEECQGTYIKYEDGNINKPLTEERKWERNLFHFDDVAKAMLTLFTVSTFEGWPGLLYVSIDSNKEDFGPIYNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFCLIMINTVTLAMKFYQQPKAYTDLLDGLNMVFTAVFALEFIFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVCSEVTTGPPVGISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIVINDETPIHRNNHFQNFPQAVLVLFRSATGEAWQEIMMACSYNPEKVLCVVTPNSTESTPDSCGSDLAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDEANAQLRAVIKKIWKRTSPKLLDQVVPPPGVDDEVTVGKFYATFLIQDYFRRFKKKKELEMKEGDKDCH-NAVTLQAGLRTLHEAGPELKRAISGNLEELLD-----DNPEPMHRRNHSLFGSVWSSMRKHGQHSFNRARSLKLNSTG-SKASPTNSIDFLPYCSLQRGGPDALNQINARSHQVVPNVAGGLSDNAMGQLSIEPRLMGAEESIPLRPLAIFGGQQLMNYRPPHKLVDGPGSGNYLHPNRDYASWGRESNGSVGTERLSHSLPGSPADRKPNFEVIGSAESLVGRVLVEQGLGKYCDPEFVRYTSREMQEALEMTREEMDRAAHQLLLQERRG 1884 HSP 2 Score: 214.927 bits (546), Expect = 1.931e-52 Identity = 177/656 (26.98%), Postives = 304/656 (46.34%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP--LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEET------CQGQFIEYKDGDLNYPIVEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK---------NQRNCIEFALNAKPVR-----------------------RYIPKNPVQYK-LW-------------------AFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
A F Q N R +C I F ++L+ I + LA P +++ N L +Y F IFT E +K+IAYGF+ H GA+ R+G+NLLD +V + +IS V ++ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ + + ++A+IG++LF GK T + +T E C + + ++ WD + ++DN +LT+F T EGW +LY S E+ G + YF+ ++I+ AFF++N+ +G + F E E A + K + R +++ A+ + R VQ++ LW V S +F + I ++ +NT LA + Y+QPE F ++ N+ F V F++E + K+ + F+ YF +N FD +V+GS+ ++ + N P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVFG + +NF +F Q+LL +F+ TGE W +M + G+ G A G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 95 ALFCLQLKNPLRKLCIDIVEWKPFEYLILLTIFANCVALAVYTPYPFGDSNQTNAYLEKVEYVFLVIFTAECVMKIIAYGFVAHQGAYLRNGWNLLDFTIVVIGMISTVLTTFMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVIFVIIIYAIIGLELFSGKLH-STCWNNVTGEMWDDPHPCGDSGFKCSTLGDEWVCDAKKYWDGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLY----SIEDAMGSTWQW------VYFVSLVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHKLREKQQIEDDMRGYLDWITQAEDIEPEADEPQTQDGKSKQQNEMESTDRLESDEGVQHESLWKRKKRDFDRANRRMRRACRKAVKSQVFYWLIIVLVFLNTGVLATEHYKQPEWLDQFQEITNMFFIVLFSMEMMLKMYSLGFQGYFVSLFNRFDCFVVIGSISEMVLTNTNVMPPLG----VSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGRFNFSAEDDKPRHNFDSFWQSLLTVFQILTGEDWNVVMYT-----GI------RAYGGVASSGV-LACIYFIILFICGNYILLNVFLAIAVDNL 723
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|148564866|gb|ABQ88369.1| (voltage-gated calcium channel alpha 1 subunit [Anopheles gambiae]) HSP 1 Score: 1532.69 bits (3967), Expect = 0.000e+0 Identity = 830/1379 (60.19%), Postives = 978/1379 (70.92%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDE-EDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN------PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPG-VLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPG-------------------------------APNVSLNVNSVQHAK--ISPTNSMDYAAQNSFRKMPDEDFPSDAAN------------IQMRPVKL----------ANGGSQDHSRPDYQYLHPYYNANGDMVAPGGSGSKSVPSSPSD-RKQSMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGSLEFADQPGWDEDDETVPLRAPPSRFMIPEHQPNDGP 1322
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN++ KPR NFD+F QSL+TVFQILTGEDWN+VMYDGIQA+GGV S+GI++S+YFIILFICGNYILLNVFLAIAVDNLADAD+L EK+E D P D EE K E T+ D + D D D D + D+Y QD + VA+ E D G +DE R RR+ S PIP+++A +F N+FRI CH++CNHS FGNI+LVCIM SSAMLAAEDPLN+NSERN+ILNYFDYFFT++FT+E+ LK+++YGF+ H GAFCRS FNLLDLLVV VSLIS FSSGAISV+KILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQFMFAVIGVQL+KGKFF C+D SKM E C G ++ Y+DG+++ P+ +ER W RN FH+D+V+K +LTLF VSTFEGWPG+LYVSIDS+EED GPIH FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNC+LDKNQRNCIEFAL AKP+RRYIPK+ +QYK+W FVTS FEY IF +I++NT++L+MKFYRQPE YT LD+LN+IFT F LEF+FKL AFRFKNYFGD WN FDFIIVLGS IDI +E+N G ++ISINFFRLFRVMRLVKLL++GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA + T+IHRNNNFQTFPQA+LVLFRSATGEAWQ+IML C PG V CD KS+DAG P GCG++ A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR+ I ++W+RT+ KLLDQ+ P G++DEVTVGKFYATFLIQDYFRRFKK+KE + ++ +++TLQAGLRTLHE GPELKRAISGNL+E+ + + E HRRN FGNV SSI RRPG PN + ++ + ++ +N+ ++ A++ + DE + N I +RP+ L AN HS+ L+ N + S PSSP + K S ++GSAE+LVGRVL QGLGKYCD D V A EMQEA+DMT EE D AAH L+ ER + PL+ P R N P
Sbjct: 585 LLRVFKVTKYWQSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFNSSVDKPRSNFDSFVQSLLTVFQILTGEDWNMVMYDGIQAYGGVASLGIIASIYFIILFICGNYILLNVFLAIAVDNLADADSLTTVEKEEGDNP----DGEEEKLSHEPTPTEHGDDGFMDHDKDNLD-------SDNDPMNISDDYDGQDSDTKIP--VAEDDEG---YEEQDTPGETFDELPTASARPRRLSELSVKKSKKPIPKSNALLIFSPTNRFRIFCHWLCNHSTFGNIILVCIMFSSAMLAAEDPLNANSERNQILNYFDYFFTSVFTIELLLKLVSYGFLFHDGAFCRSAFNLLDLLVVCVSLISMFFSSGAISVIKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLYKGKFFSCSDGSKMQESECHGTYLVYEDGNVDKPVSKERYWSRNRFHFDDVSKAMLTLFTVSTFEGWPGLLYVSIDSHEEDSGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPIRRYIPKHRIQYKVWWFVTSQPFEYMIFVLIMINTITLSMKFYRQPEIYTEVLDLLNLIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEVNISKGMKGGSSIISINFFRLFRVMRLVKLLARGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMDDETSIHRNNNFQTFPQAVLVLFRSATGEAWQDIMLDCSSRPGEVNCDAKSDDAGSPEGCGSNIAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLKIKTEGNIDDANAELRNTIKQIWKRTNPKLLDQVVPPPGVDDEVTVGKFYATFLIQDYFRRFKKRKENDSKLNSDQNKRRTMTLQAGLRTLHEAGPELKRAISGNLEEIGD-----DNPEPSHRRNHTLFGNVWSSI--RRPGPFGSKQKWNSNNNKMSATSMAASAAMHTVLSGPNDGSSQTEMKPKQNCVNSSNTYNHVAESILHAVHDEPIGRNYGNGLNIGNAGHTGEIPLRPLVLDDSSILKRANANANIGSHSK-----LYSQINIIKLPILRKDRLIHSTPSSPQETHKLSAEVIGSAESLVGRVLAQQGLGKYCDLDLVHCAQMEMQEALDMTQEEMDLAAHELMLEERF------------KRKNCAPLKGQPKRGTNTRKNKNQQP 1891 HSP 2 Score: 217.238 bits (552), Expect = 3.643e-53 Identity = 185/680 (27.21%), Postives = 310/680 (45.59%), Query Frame = 0
Query: 261 PVNPTMPIPEAS----------AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPL-NSNS-ERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIE-----YKDG----DLNYPIVEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK-NQRNCIEFAL-----------------NAKPVRRYIPKN--------------PVQYKLWA--------------------FVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAM--AEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
P+ P P A A F N R +C I F ++L+ I + LA P NS+S N L +Y F IFT E +K+IAYGFILHPG++ R+G+N+LD +V + +IS S+ VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK +S ET G+ ++ +DG ++ +V W+ F ++DN +LT+F T EGW +LY D+ + YFI ++I+ AFF++N+ +G + F E A + K ++ IE L +A ++ KN +Q++ W V S F + I ++ +NT LA + YRQP F + N+ F FT+E + K+ + F+ YF +N FD +V+GS+ ++ + +I P + ++ R R++R+ K+ + + L+ + S Q++ + +L+ L I+ ++GMQVFG + +NF +F Q+LL +F+ TGE W +M + + G A G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 70 PIAPKKPTRRAGVKPQPDRPMRALFCLTRTNPLRKLCIAIVEWKPFEYLILLTIFANCVALAVYTPFPNSDSNSTNAALEKIEYIFLVIFTAECIMKLIAYGFILHPGSYLRNGWNILDFTIVVIGMISTALSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKL----HKSCFHNET--GEIMDDPHPCGEDGFHCDTISPEMVCRYYWEGPNFGITNFDNFGLSMLTVFQCVTLEGWTDMLYYIEDAMGSSWQWV----------YFISMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIDPDNDASGMQEGKMKNIIELDSSDNVGEDGEIQHESWVARKRKTIDRINRRLRRACRKAVKSQAFYWLIIILVFLNTGVLATEHYRQPPWLDDFQEYTNMFFVALFTMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEMILTSTQIMPP---LGVSVLRCVRLLRVFKVTKYWQSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFNSSVDKPRSNFDSFVQSLLTVFQILTGEDWNMVMY-----------DGIQAYGGVASLGI-IASIYFIILFICGNYILLNVFLAIAVDNL 718
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|340710242|ref|XP_003393703.1| (muscle calcium channel subunit alpha-1 isoform X1 [Bombus terrestris] >gi|1185567568|ref|XP_020721588.1| muscle calcium channel subunit alpha-1 isoform X1 [Bombus terrestris]) HSP 1 Score: 1502.65 bits (3889), Expect = 0.000e+0 Identity = 834/1363 (61.19%), Postives = 981/1363 (71.97%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADAL----------------EDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPG-VLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV----SLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDFP------------------SDAA---------------NIQMRPV-----------------KLANG-GSQDHSRPDYQYLHPYYNANGDMVAPGGSGSKSVPSSPSDRKQSMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGS-LEFADQPGWDE 1297
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN+ L KPR NFD+F+QSL+TVFQILTGEDWN VMYDGI+A+GGV S G+L+ YFIILFICGNYILLNVFLAIAVDNLADA++L D+ + DD G ED ++ + + Y+ T + S+D + NE G D++ R RRM + T PIP S+FFLF N+FR+ CH++ NHSYF N++LVCIM+SSAMLAAEDPL ++S RN+IL FDYFFTT+FT+E+CLK+I++GFI+H GAFCRS FNLLDLLVV SLIS FSSGA SVVK+LRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQF+FAVIGVQLFKGKFF C+D SKMT++ CQG ++EY +GD+N P+++ER W + FH+D+VAK +LTLF VSTFEGWP +L VSIDSN+EDHGPIH FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKFYRQPE YT LDVLN+IFT F LEFIFKL AFRFKNYFGD WN FDFIIVLGS IDI +E+NPG +ISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA + T+I RNNNFQ+FPQA+LVLFRSATGEAWQ+IML C PG V CD S++ + GCG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G +DEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLHE GPELKRAISGNL+E+++ + E MHRRN FG+V SS+ R G N SL VNS K SPTNS+D+ +S ++ D P SD+A NI +RP+ K+ +G GS ++ P+ +Y+ +NG + A S S+P SP+DRK + ++GSAE+LVGRVL +QGLGKYCDPDFVR SREMQEA+DMT EE D+AAH+LL ER+G L + Q G D+
Sbjct: 578 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFSDLQNKPRHNFDSFWQSLLTVFQILTGEDWNAVMYDGIRAYGGVSSFGMLACFYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEGGEKNKQHSASASRDEVSGEQGDDGGEGTGAEDEGGGTDLEHDPNERMEDYEAAVDTETSE----KSDDTNNHAKVRLNIESDEEVEEEEEVEHNEMHDDGADHEVSA---RPRRMSEFNMATKTEPIPAGSSFFLFSQTNRFRVFCHWLSNHSYFSNMILVCIMISSAMLAAEDPLRASSYRNQILLNFDYFFTTVFTIEICLKMISFGFIIHDGAFCRSAFNLLDLLVVCSSLISMSFSSGAFSVVKVLRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTSLLQFVFAVIGVQLFKGKFFYCSDASKMTKDECQGTYLEYDNGDINKPVMKERSWVQQRFHFDDVAKAMLTLFTVSTFEGWPSLLDVSIDSNKEDHGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFTLIMINTVTLAMKFYRQPEIYTEALDVLNMIFTAVFALEFIFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEVNPGSTIISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIIMLFFIYAVIGMQVFGKIAIDDDTSIDRNNNFQSFPQAVLVLFRSATGEAWQDIMLDCSAQPGKVKCDPNSDEVNNHNGCGSDIAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGGDDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGNKESHNTVTLQAGLRTLHEAGPELKRAISGNLEELLD-----DNPEPMHRRNHSLFGSVWSSM---RKGHHNFNRARSLKVNST--TKASPTNSIDFLPYSSLQRGSGHDPPNQITARSHQVVPNVAGGLSDSAMNQMGMDPKLTGIEENIPLRPLAVFGNPVQQQSYHHTPYKVFDGPGSGNYLHPNSEYVSWAGESNGSIGAE--RLSHSLPGSPADRKPNFEVIGSAESLVGRVLVEQGLGKYCDPDFVRYTSREMQEALDMTREEMDRAAHQLLLQERRGQPLSYQLQQGADQ 1888 HSP 2 Score: 133.65 bits (335), Expect = 1.881e-27 Identity = 97/320 (30.31%), Postives = 155/320 (48.44%), Query Frame = 0
Query: 273 AFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDP--LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEET------CQGQFIEYKDGDLNYPIVEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
A F N R +C + F ++L+ I + LA P ++ N+ L +Y F IFT+E +K+IAYGF+ HPGA+ R+G+N+LD +V + ++S V S VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK T R +T+ C + + Y R W+ + ++DN +LT+F T EGW +LY N ED YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 83 ALFCLPLKNPVRKMCIDVIEWKPFEWLILMTIFANCIALAVYTPYPFGDSNLTNQYLEKIEYIFLVIFTLECVMKIIAYGFVAHPGAYLRNGWNILDFSIVVIGMVSTVLSILMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHK-TCRHNVTDAIMDNPIPCGSGGFQCDNVGPEY-YCSRRFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLY-----NIED-----AMGSSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 390 HSP 3 Score: 105.916 bits (263), Expect = 4.728e-19 Identity = 72/254 (28.35%), Postives = 130/254 (51.18%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S +F + I ++ +NT LA + Y QP F ++ N+ F F++E + K+ + F+ YF +N FD +V+GS+ ++ + + P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVF GK + N R +NF +F Q+LL +F+ TGE W +M + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 475 VKSQVFYWLIIVLVFLNTGVLATEHYNQPHWLDDFQEITNMFFIALFSMEMMLKMYSLGFQGYFVSLFNRFDCFVVIGSITEMILTNTHVMPPLG----VSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFSDLQNKPR-HNFDSFWQSLLTVFQILTGEDWNAVMY-----------DGIRAYGGVSSFGM-LACFYFIILFICGNYILLNVFLAIAVDNL 711 HSP 4 Score: 92.8189 bits (229), Expect = 4.259e-15 Identity = 125/586 (21.33%), Postives = 238/586 (40.61%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNY------------DPLAQKPRG-------------------------NFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQY-DEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEE---DEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIA-FFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + + +P+ G NFDNF +++TVFQ +T E W V+Y+ A G +YFI + I G + ++N+ L + + K+ + GD K+ Q DD Y D + T +D P DE QD K+ +++ +G DEE E R+ + VN M R C F +++V + +++ +LA E N + + FF +F++E+ LK+ + GF G F S FN D VV S+ + ++ + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF + D + ++ ++D+ + LLT+F + T E W ++Y I + +G + F ++A FYFI + I + ++N+F+ +
Sbjct: 222 SLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCRHNVTDAIMDNPIPCGSGGFQCDNVGPEYYCSRRFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLYNIEDAMGSS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS---------KEREKAKARGDFH------------KLREKQQIEDDLRGYLDWI--TQAED--IEPETDEPKMQDGK-------TKQQNEMESTDQLEG-----DEEGVQQESLWRRKKLDFDRVNRRM-----------------RRACRKAVKSQVFYWLIIVLVFLNTGVLATEH-YNQPHWLDDFQEITNMFFIALFSMEMMLKMYSLGF---QGYFV-SLFNRFDCFVVIGSITEMILTNTHVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF-------------------NFSD-----------LQNKPRHNFDSFWQSLLTVFQILTGEDWNAVMYDGIRA----YGGVSSF-GMLACFYFIILFICGNYILLNVFLAIAV 708 HSP 5 Score: 88.9669 bits (219), Expect = 6.568e-14 Identity = 91/393 (23.16%), Postives = 163/393 (41.48%), Query Frame = 0
Query: 599 KNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEG----YTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GK-----------------------------IAPQ--------EGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKR 945
KNPV+ + FE+ I I N ++LA+ + P G +L+ + IF V FTLE + K+ A+ F Y + WN DF IV+ ++ ++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK + P+ EG N NF F A+L +F+ T E W +++ + EDA G+ + + YFIS ++ +F ++NL + V+ F G H +R + + D +G ++D +T I P K+ + + +ME DE +W+R
Sbjct: 90 KNPVRKMCIDVIEWKPFEWLILMTIFANCIALAV-YTPYPFGDSNLTNQYLEKIEYIFLVIFTLECVMKIIAYGFVAHPGAYLRNGWNILDFSIVVIGMVSTVLSILMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCRHNVTDAIMDNPIPCGSGGFQCDNVGPEYYCSRRFWEGPNWGI-TNFDNFGLAMLTVFQCVTLEGWTDVLYNI------------EDA-----MGSSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEDDLRG---YLDWITQAEDIEPETDEPKMQDGKTKQQNEMESTDQLEGDEEGVQQESLWRR 455
BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Match: gi|815913115|ref|XP_003490120.2| (muscle calcium channel subunit alpha-1 isoform X4 [Bombus impatiens]) HSP 1 Score: 1501.49 bits (3886), Expect = 0.000e+0 Identity = 834/1363 (61.19%), Postives = 981/1363 (71.97%), Query Frame = 0
Query: 8 LLNSPGIGRYWKALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGARFNYDPLAQKPRGNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADAL----------------EDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQYDEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEEDEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIAFFMVNIFVGFVIVTFQNEGESAYKNCELDKNQRNCIEFALNAKPVRRYIPKNPVQYKLWAFVTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPG-VLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKRTPQKVVDARLVSMNMPLNSDGSVNFNATLFALVRTSLSIKTEGNIDEANEELRHQILKVWRRTDIKLLDQICPGAGLEDEVTVGKFYATFLIQDYFRRFKKKKETKTLTEHPDHEEQSVTLQAGLRTLHEKGPELKRAISGNLDEMIEAMDLTNEEETMHRRNLPFFGNVVSSIKNRRPGAPNV----SLNVNSVQHAKISPTNSMDYAAQNSFRKMPDEDFP------------------SDAA---------------NIQMRPV-----------------KLANG-GSQDHSRPDYQYLHPYYNANGDMVAPGGSGSKSVPSSPSDRKQSMPIVGSAENLVGRVLRDQGLGKYCDPDFVRAASREMQEAMDMTPEEFDQAAHRLLQAERQGS-LEFADQPGWDE 1297
LL + +YW++LSNLV SL NSIQSIASLLLLLFLF+ IFALLGMQVFG +FN+ L KPR NFD+F+QSL+TVFQILTGEDWN VMYDGI+A+GGV S G+L+ YFIILFICGNYILLNVFLAIAVDNLADA++L D+ + DD G ED ++ + + Y+ T + S+D + NE G D++ R RRM + T PIP S+FFLF N+FR+ CH++ NHSYF N++LVCIM+SSAMLAAEDPL ++S RN+IL FDYFFTT+FT+E+CLK+I++GFI+H GAFCRS FNLLDLLVV SLIS FSSGA SVVK+LRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVT LLQF+FAVIGVQLFKGKFF C+D SKMT++ CQG ++EY +GD+N P+++ER W + FH+D+VAK +LTLF VSTFEGWP +L VSIDSN+EDHGPIH FRPIVA +Y IYIIIIAFFMVNIFVGFVIVTFQNEGE YKNCELDKNQRNCIEFAL AKPVRRYIPK+ +QYK+W FVTS FEYTIF +I++NT++LAMKFYRQPE YT LDVLN+IFT F LEFIFKL AFRFKNYFGD WN FDFIIVLGS IDI +E+NPG +ISINFFRLFRVMRLVKLLS+GEGIRTLLWTF KSFQALP+VA+LI ++FFIY V+GMQVFGKIA + T+I RNNNFQ+FPQA+LVLFRSATGEAWQ+IML C PG V CD S++ + GCG+D A+PYFISF+V+CSFLI+NLFVAVIMDNFDYLTRDWSILGPHHLDEF+RLWSEYDPDAKGRIKH+DVVTLLRKISPPLGFGKLCPHRVACK RLVSMNMPLNSDG+V FNATLFA+VRTSL IKTEGNID+AN ELR I K+W+RT KLLDQ+ P G +DEVTVGKFYATFLIQDYFRRFKK+KE + L + +VTLQAGLRTLHE GPELKRAISGNL+E+++ + E MHRRN FG+V SS+ R G N SL VNS K SPTNS+D+ +S ++ D P SD+A NI +RP+ K+ +G GS ++ P+ +Y+ +NG + A S S+P SP+DRK + ++GSAE+LVGRVL +QGLGKYCDPDFVR SREMQEA+DMT EE D+AAH+LL ER+G L + Q G D+
Sbjct: 483 LLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFSDLQNKPRHNFDSFWQSLLTVFQILTGEDWNAVMYDGIRAYGGVSSFGMLACFYFIILFICGNYILLNVFLAIAVDNLADAESLTAIEKEAEEGGEKNKQHSASASRDEVSGEQGDDGGEGTGAEDEGGGTDLEHDPNETMEDYEAAVDTETSE----KSDDTNNHAKVRLNIESDEEVEEEEEVEHNEMHDDGADHEV---SARPRRMSEFNMATKTEPIPAGSSFFLFSQTNRFRVFCHWLSNHSYFSNMILVCIMISSAMLAAEDPLRASSYRNQILLNFDYFFTTVFTIEICLKMISFGFIIHDGAFCRSAFNLLDLLVVCSSLISMSFSSGAFSVVKVLRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTSLLQFVFAVIGVQLFKGKFFYCSDASKMTKDECQGTYLEYDNGDINKPVMKERSWIQQRFHFDDVAKAMLTLFTVSTFEGWPSLLDVSIDSNKEDHGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCELDKNQRNCIEFALKAKPVRRYIPKHRIQYKVWWFVTSQPFEYTIFTLIMINTVTLAMKFYRQPEIYTEALDVLNMIFTAVFALEFIFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEVNPGSTIISINFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIIMLFFIYAVIGMQVFGKIAIDDDTSIDRNNNFQSFPQAVLVLFRSATGEAWQDIMLDCSAQPGKVKCDPLSDEVNNHNGCGSDIAFPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRVACK--------------------------------RLVSMNMPLNSDGTVLFNATLFAVVRTSLRIKTEGNIDDANAELRAVIKKIWKRTSPKLLDQVVPPPGGDDEVTVGKFYATFLIQDYFRRFKKRKE-QELKDGNKESHNTVTLQAGLRTLHEAGPELKRAISGNLEELLD-----DNPEPMHRRNHSLFGSVWSSM---RKGHHNFNRARSLKVNST--TKASPTNSIDFLPYSSLQRGSGHDPPNQITARSHQVVPNVAGGLSDSAMNQMGMDPKLTGIEENIPLRPLAVFGNPVQQQSYHHTPYKVFDGPGSGNYLHPNSEYVSWAGESNGSIGAE--RLSHSLPGSPADRKPNFEVIGSAESLVGRVLVEQGLGKYCDPDFVRYTSREMQEALDMTREEMDRAAHQLLLQERRGQPLSYQLQQGADQ 1793 HSP 2 Score: 131.724 bits (330), Expect = 6.718e-27 Identity = 96/298 (32.21%), Postives = 150/298 (50.34%), Query Frame = 0
Query: 296 FGNIVLVCIMVSSAMLAAEDP--LNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV--VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEET------C-QGQFIEYKDGDLNYPIVEERIWDRNAF---HYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIII-AFFMVNIFVGFVIVTFQNEGESAYKNCELDK 578
F ++L+ I + LA P ++ N+ L +Y F IFT+E +K+IAYGF+ HPGA+ R+G+N+LD +V + ++S V S VK LR RVLRPLR ++ L+ V+ ++ A+ + +I L+ L + ++A+IG++LF GK T R +T+ C G F Y G Y R W+ + ++DN +LT+F T EGW +LY N ED YFI ++I+ AFF++N+ +G + F E E A + K
Sbjct: 11 FEWLILMTIFANCIALAVYTPYPFGDSNLTNQYLEKIEYIFLVIFTLECVMKIIAYGFVAHPGAYLRNGWNILDFSIVVIGMVSTVLSILMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHK-TCRHNVTDAIMDNPIPCGSGGFQCYNVGPEYY--CSRRFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLY-----NIED-----AMGSSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK 295 HSP 3 Score: 105.145 bits (261), Expect = 7.160e-19 Identity = 72/254 (28.35%), Postives = 130/254 (51.18%), Query Frame = 0
Query: 610 VTSPIFEYTIFAMILVNTLSLAMKFYRQPEGYTAFLDVLNVIFTVFFTLEFIFKLGAFRFKNYFGDPWNAFDFIIVLGSLIDIAMAEIN---PGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GKIAPQEGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 859
V S +F + I ++ +NT LA + Y QP F ++ N+ F F++E + K+ + F+ YF +N FD +V+GS+ ++ + + P G ++ R R++R+ K+ + L+ + S Q++ + +L+ L I+ ++GMQVF GK + N R +NF +F Q+LL +F+ TGE W +M + G + G A YFI F+ ++++LN+F+A+ +DN
Sbjct: 380 VKSQVFYWLIIVLVFLNTGVLATEHYNQPHWLDDFQEITNMFFIALFSMEMMLKMYSLGFQGYFVSLFNRFDCFVVIGSITEMILTNTHVMPPLG----VSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFSDLQNKPR-HNFDSFWQSLLTVFQILTGEDWNAVMY-----------DGIRAYGGVSSFGM-LACFYFIILFICGNYILLNVFLAIAVDNL 616 HSP 4 Score: 92.4337 bits (228), Expect = 6.860e-15 Identity = 126/586 (21.50%), Postives = 237/586 (40.44%), Query Frame = 0
Query: 20 ALSNLVNSLTNSIQSIASLLLLLFLFMCIFALLGMQVFGAR---------------------------FNYDPLAQKPR----------GNFDNFFQSLITVFQILTGEDWNVVMYDGIQAFGGVKSIGILSSLYFIILFICGNYILLNVFLAIAVDNLADADALEDEEKDEDDPPEDGDDDEEGKDGGEDGETKIPMDQYYPDDYDQY-DEVGRTYGQDGYAVPSEDEYYQQDPSQVVGQRVAKRPRSPSLSNEFDGIGYDYDEE---DEGDRMRRMISESPVNPTMPIPEASAFFLFQHDNQFRIVCHYICNHSYFGNIVLVCIMVSSAMLAAEDPLNSNSERNKILNYFDYFFTTIFTVEVCLKVIAYGFILHPGAFCRSGFNLLDLLVVAVSLISFVFSSGAISV---VKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTLLLQFMFAVIGVQLFKGKFFMCTDRSKMTEETCQGQFIEYKDGDLNYPIVEERIWDRNAFHYDNVAKGLLTLFVVSTFEGWPGILYVSIDSNEEDHGPIHGFRPIVAIFYFIYIIIIA-FFMVNIFVGFVI 560
+L ++NS+ ++ + + LL+ + I+A++G+++F + +N P R NFDNF +++TVFQ +T E W V+Y+ A G +YFI + I G + ++N+ L + + K+ + GD K+ Q DD Y D + T +D P DE QD K+ +++ +G DEE E R+ + VN M R C F +++V + +++ +LA E N + + FF +F++E+ LK+ + GF G F S FN D VV S+ + ++ + V +LR +R+LR + + L ++V ++ ++++I +++L+ L +FA++G+Q+F GKF + D + ++ ++D+ + LLT+F + T E W ++Y I + +G + F ++A FYFI + I + ++N+F+ +
Sbjct: 127 SLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCRHNVTDAIMDNPIPCGSGGFQCYNVGPEYYCSRRFWEGPNWGITNFDNFGLAMLTVFQCVTLEGWTDVLYNIEDAMGSS-----WQWIYFISMVILGAFFVMNLILGVLSGEFS---------KEREKAKARGDFH------------KLREKQQIEDDLRGYLDWI--TQAED--IEPETDEPKMQDGK-------TKQQNEMESTDQLEG-----DEEGVQQESLWRRKKLDFDRVNRRM-----------------RRACRKAVKSQVFYWLIIVLVFLNTGVLATEH-YNQPHWLDDFQEITNMFFIALFSMEMMLKMYSLGF---QGYFV-SLFNRFDCFVVIGSITEMILTNTHVMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF-------------------NFSD-----------LQNKPRHNFDSFWQSLLTVFQILTGEDWNAVMYDGIRA----YGGVSSF-GMLACFYFIILFICGNYILLNVFLAIAV 613 HSP 5 Score: 84.3445 bits (207), Expect = 1.632e-12 Identity = 87/377 (23.08%), Postives = 157/377 (41.64%), Query Frame = 0
Query: 615 FEYTIFAMILVNTLSLAMKFYRQPEG----YTAFLDVLNVIFTVFFTLEFIFKLGAFRF----KNYFGDPWNAFDFIIVLGSLIDIAMAEINPGGNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVF-GK-----------------------------IAPQ--------EGTNIHRNNNFQTFPQALLVLFRSATGEAWQEIMLSCKKDPGVLCDEKSEDAGDPAGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNFDYLTRDWSILGPHHLDEFVRLWSEYDPDAKGRIKHVDVVTLLRKISPPLGFGKLCPHRVACKIKMEGPIDQANDELKQIVLRIWKR 945
FE+ I I N ++LA+ + P G +L+ + IF V FTLE + K+ A+ F Y + WN DF IV+ ++ ++ + G + R FRV+R ++L+S ++ +L + ++ L +A+L+ + IY ++G+++F GK + P+ EG N NF F A+L +F+ T E W +++ + EDA G+ + + YFIS ++ +F ++NL + V+ F G H +R + + D +G ++D +T I P K+ + + +ME DE +W+R
Sbjct: 11 FEWLILMTIFANCIALAV-YTPYPFGDSNLTNQYLEKIEYIFLVIFTLECVMKIIAYGFVAHPGAYLRNGWNILDFSIVVIGMVSTVLSILMKEG--FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMIPLLHIALLVLFVIIIYAIIGLELFSGKMHKTCRHNVTDAIMDNPIPCGSGGFQCYNVGPEYYCSRRFWEGPNWGI-TNFDNFGLAMLTVFQCVTLEGWTDVLYNI------------EDA-----MGSSWQWIYFISMVILGAFFVMNLILGVLSGEFSKEREKAKARGDFHK---LREKQQIEDDLRG---YLDWITQAEDIEPETDEPKMQDGKTKQQNEMESTDQLEGDEEGVQQESLWRR 360 The following BLAST results are available for this feature:
BLAST of voltage-gated calcium channel alpha 1 subunit vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 17
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BLAST of voltage-gated calcium channel alpha 1 subunit vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of voltage-gated calcium channel alpha 1 subunit vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold81_size397536:309425..324749- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold81_size397536-snap-gene-2.22 ID=maker-scaffold81_size397536-snap-gene-2.22|Name=voltage-gated calcium channel alpha 1 subunit|organism=Tigriopus kingsejongensis|type=gene|length=15325bp|location=Sequence derived from alignment at scaffold81_size397536:309425..324749- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'voltage-gated calcium channel alpha 1 subunit' has the following synonyms
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