conserved hypothetical protein, maker-scaffold871_size86487-snap-gene-0.25 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000007492 (supercontig:LSalAtl2s:LSalAtl2s430:139689:146390:-1 gene:EMLSAG00000007492 transcript:EMLSAT00000007492 description:"maker-LSalAtl2s430-augustus-gene-1.25") HSP 1 Score: 890.567 bits (2300), Expect = 0.000e+0 Identity = 501/908 (55.18%), Postives = 630/908 (69.38%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQALASPVHSDPPA---------PRSPPSP--LETAMAPR-LENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSEST--QSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPS-VSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPE-IFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVG-TKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPR 894
MPI+ + RAAL LLKE + + S + ++ DL TLISVLESPVF +ILNIQDSL ELK QV LHPSILP+DFDI +G+L+LNLP ASP + PPA PPS +A + +++E + + +QGR++K I L KPEGHSLGFSVVGL+SE KGELGIY+QEIQP G+A +DG+LIEGDQILAIDG+ LDSNISH QAINILQ+A G V LVVARGS + +++ ++P E+ C VEVIEL NNG+G GFG++ GQ+ GVVVKT++ +GVAD DGRL AGD+ILQIN+HWLQG G EQVA VLR +GN V+LIVAR + + + +P+LP L R ELE HLQ ++ V A++ P +L N +I SH QTT+P +A ++ MPEMETLDVEL+KD+QGLGITIAGYTCEREELSGIFVKSVTDGSAA +SGMV VNDQI+EVDG SIQG++NQQAVEMLRSTG+SV+L+L+RYVHGLKFEQLQQAIANSQSN+ T+P+ + Q++ +P + ++++ + ++ + E + + E + + I + W + +G + I++A ++K + GGLGISLEGTVEKVDGEE NPHHYIRSVLP+GPVG NG+LKSGDELLEVNG+KL+GLYHTDVV+ILKDLP+ V++VCAR +TSV + E + ER+VKAKSD SISS GT T ++ KSRSLEPLTSLAMW++ +L+I+LIKG++GLGFSILDYQDP+NKDETVIVIRSLVPGGV+QQDGRLIPGDRLM VN ++L HA+LDEAVQALKGAP+
Sbjct: 1 MPISGETRAALELLKEVEKKVKESESL---SSNAQIKTDLNTLISVLESPVFTSILNIQDSLRELKRQVHLHPSILPADFDITPSGELVLNLPQ----ASPTSTSPPALVQLSSSNISXVPPSHNGFSSAEGKQGIKSEVELDSF-EDIAQGREIKRIKLYKPEGHSLGFSVVGLRSEFKGELGIYLQEIQPDGLAGKDGRLIEGDQILAIDGKSLDSNISHQQAINILQRADGYVDLVVARGSEDITQNKIDETPAES-----------------CVVEVIELVNNGSGLGFGIIGGQKK-GVVVKTILPDGVADSDGRLRAGDFILQINEHWLQGVGSEQVAAVLRGTGNVVKLIVARPITSXNKDXSRS-LPVLPILSLENREELEAHLQ-----KTTSVGASTREEAPNIDL-NQLI-------------SH------QTTTP---------SAGVEL-----------MPEMETLDVELIKDSQGLGITIAGYTCEREELSGIFVKSVTDGSAASRSGMVAVNDQIVEVDGNSIQGYTNQQAVEMLRSTGKSVKLKLIRYVHGLKFEQLQQAIANSQSNTPITSPTQMIQLSPHISPTKSLHIEAIESKNQLNNDEVNENAVYEINNRVDSFEGELDPIIEREIIQKWSDYMGVQYEIVVAQLKKFQSTGGLGISLEGTVEKVDGEEHNPHHYIRSVLPDGPVGHNGLLKSGDELLEVNGQKLIGLYHTDVVSILKDLPVWVRIVCAR----NTSVDPPRSKLISNNNTSDESTVSVERLVKAKSDGSISSMGTNTGNNESTSSMNSKSRSLEPLTSLAMWAEEVLDIDLIKGDKGLGFSILDYQDPLNKDETVIVIRSLVPGGVAQQDGRLIPGDRLMKVNQVDLCHATLDEAVQALKGAPK 832 HSP 2 Score: 881.322 bits (2276), Expect = 0.000e+0 Identity = 510/886 (57.56%), Postives = 609/886 (68.74%), Query Frame = 0
Query: 1335 SNVFSSQNWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPV-VTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDPVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLS--QFPPEP-HKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGD--ESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQG------VGGM-----HSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
S + S++WGPER V + R PN+GLGISIVGGKV+P + DG S + V+GIFIKNVLEGSPAG + L TGDRIL VDG+DL A+H++AVDVIRQS VE + D+ + +++++ P I++ PP FAN +S+ +E+E + G G TLSNG VIDK SA+FL K + + EEED++ Y+ KI +KY + G V LNKG GLGISLAGHKDR++M+V+ICGL P GNA RD R+++GD +LEVNG V+++R HLN SS IKN PDS VTF+LL + AVKPLS QFP +K+NPIERY KY GL ++ I K +QGLGIMIIEGKH + G+GVFVSDLQP S AD AGL GDMIL VNGEDFVG +YESAAR+LK+ +G+I M VANP +PP T TE KP LPPKP +APKP +SP H P V ++KP+ASPR K E PATC+I PG DT IEI+KDKD++GKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRL+PGDQILEV++++LRNV+HS+ALHALRQTPN+VRL+IHREDDEIYET +VEL K+KDRGLGLSIVGKKSGPGV+ISEVVKGGAADSDGRLVQGDQII VNG+DLTNSSQE+AAPILKM QGKI M+VRRL VGNR+Q V G+ HS DS+ S + GT KT+ L+RG+ GLGFSIVGGFGSPHGDMPIYVKTVF TGAAA+HGGLKRGDQI+SVN LEGL+H DAVNILKNC+G V +QILS
Sbjct: 1150 SKAYKSKHWGPEREVTVFRAPNKGLGISIVGGKVDPPSSDDGDSSSGSLNASVSGIFIKNVLEGSPAGQTGQLCTGDRILEVDGNDLRTASHERAVDVIRQSNNPVEEE-------EERDMVEGDANQGEEGNDNISM-------PKDKIEA-PPEFAN---------------------------TSTDKEEEEEESEEGELSGTETLSNGFVIDKASASFLDKSSIDDEEEDDYRYTMDKIRRKYGKFPGKLSLVRLNKGIXGLGISLAGHKDRNQMAVFICGLIPQGNAARDGRIKIGDSVLEVNGKVLHNRCHLNASSLIKNFPDSAVTFVLLTPEEGGSQCAVKPLSANQFPHGLLNKNNPIERYK-KYAGLRQIRIKKEDQGLGIMIIEGKHTEAGTGVFVSDLQPDSAADKAGLLVGDMILCVNGEDFVGASYESAARVLKSLDGIITMCVANP------------NVESAPP-------TAATEEIKKPILPPKPPVAPKPPGLSPTHKPSTV---------------------------LKKPLASPRVKEEKPSEKQLPATCDIVPGMDTTIEITKDKDDDGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLRPGDQILEVMNDNLRNVTHSYALHALRQTPNKVRLVIHREDDEIYETFEVELIKRKDRGLGLSIVGKKSGPGVYISEVVKGGAADSDGRLVQGDQIILVNGNDLTNSSQEEAAPILKMAQGKITMKVRRLXVGNRQQQRQAPAPVSGLHHVSQHSNDSN--SAMAPIINGTPKTIRLERGEQGLGFSIVGGFGSPHGDMPIYVKTVFQTGAAAEHGGLKRGDQIVSVNNISLEGLTHQDAVNILKNCEGTVTIQILS 1944 HSP 3 Score: 101.293 bits (251), Expect = 2.341e-21 Identity = 99/339 (29.20%), Postives = 153/339 (45.13%), Query Frame = 0
Query: 1921 KPMG--LGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHREDDEIY---------ETLDVELNK--KKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR------------------LRVGNR--------KQGVGGMHSRDSS--------IPSNVPGATGGT-----MKT--VELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
KP G LG S+VG G I++ E+ G A KDGRL GDQIL + + L N+SH A++ L++ V L++ R ++I E+ VE+ + GLG I+G + GV + ++ G ADSDGRL GD I+ +N H L E A +L+ T + + V R L + NR K G +R+ + I + G M+T VEL + GLG +I G I+VK+V D AA+ G + DQI+ V+ ++G ++ AV +L++ +V L+++
Sbjct: 154 KPEGHSLGFSVVGLRSEFKGELGIYLQEIQPDGLAGKDGRLIEGDQILAIDGKSLDSNISHQQAINILQRADGYVDLVVARGSEDITQNKIDETPAESCVVEVIELVNNGSGLGFGIIGGQK-KGVVVKTILPDGVADSDGRLRAGDFILQINEHWLQGVGSEQVAAVLRGTGNVVKLIVARPITSXNKDXSRSLPVLPILSLENREELEAHLQKTTSVGASTREEAPNIDLNQLISHQTTTPSAGVELMPEMETLDVELIKDSQGLGITIAGYTCEREELSGIFVKSVTDGSAASRSGMVAVNDQIVEVDGNSIQGYTNQQAVEMLRSTGKSVKLKLI 491 HSP 4 Score: 80.8777 bits (198), Expect = 4.406e-15 Identity = 97/404 (24.01%), Postives = 159/404 (39.36%), Query Frame = 0
Query: 1599 LGISLAGHKDRSK--MSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTV-IEISKDKDENGKPMGLGLSIVGGSDTL--LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHR 1996
LG S+ G + K + +Y+ + P G A +D R+ GD IL ++G S N+ IL R+D +D + + E N I+ + + + ++ GLG II G+ GV V + P AD G L+ GD IL +N GV E A +L+ + ++K+IVA PI D ++ P P ++ +L GA + P I + +++ + TT A E+ P +T+ +E+ KD GLG++I G + L IF+ V + AA + G + DQI+EV ++ ++ A+ LR T V+L + R
Sbjct: 160 LGFSVVGLRSEFKGELGIYLQEIQPDGLAGKDGRLIEGDQILAIDG-----------KSLDSNISHQQAINILQRADGYVDLVVARG-----SEDITQNKIDETPAESCVVEVIELVNNGSGLGFGIIGGQK----KGVVVKTILPDGVADSDGRLRAGDFILQINEHWLQGVGSEQVAAVLRGTGNVVKLIVARPITSXNKDXSRSL------------PVLPILSLENREELEAHLQKTTSVGASTREEAPNI---------DLNQLISHQTTT------------------------PSAGVELMPEMETLDVELIKDS------QGLGITIAGYTCEREELSGIFVKSVTDGSAASRSGMVAVNDQIVEVDGNSIQGYTNQQAVEMLRSTGKSVKLKLIR 492 HSP 5 Score: 78.9518 bits (193), Expect = 1.678e-14 Identity = 60/199 (30.15%), Postives = 95/199 (47.74%), Query Frame = 0
Query: 149 IALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQV-----EVIELTNNGAGFGFGVMAG----QQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLR 337
+ L K + LG S+VG KS G+Y+ E+ G A DG+L++GDQI+ ++G L +N S +A IL+ A+G++ + V R + Q+P + L NS + + I L G GF ++ G + VKTV Q G A + G L GD I+ +N+ L+G + N+L+
Sbjct: 1739 VELIKRKDRGLGLSIVGKKSGP----GVYISEVVKGGAADSDGRLVQGDQIILVNGNDL-TNSSQEEAAPILKMAQGKITMKVRRLXVGNRQQQRQAPAPVSGLHHVSQHSNDSNSAMAPIINGTPKTIRLERGEQGLGFSIVGGFGSPHGDMPIYVKTVFQTGAAAEHGGLKRGDQIVSVNNISLEGLTHQDAVNILK 1932 HSP 6 Score: 77.0258 bits (188), Expect = 6.557e-14 Identity = 59/198 (29.80%), Postives = 91/198 (45.96%), Query Frame = 0
Query: 2007 VELNKKKDRGLGLSIVGKKSG----PGVFISEVVKGGAADSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
++L K + LG S+VG +S G+++ E+ G A DGRL++GDQI+ ++G L +N S + A IL+ G + + V R G + P A ++ +EL GLGF I+GG + VKT+ G A G L+ GD IL +N L+G+ +L+ V L
Sbjct: 150 IKLYKPEGHSLGFSVVGLRSEFKGELGIYLQEIQPDGLAGKDGRLIEGDQILAIDGKSLDSNISHQQAINILQRADGYVDLVVAR-----------GSEDITQNKIDETP-AESCVVEVIELVNNGSGLGFGIIGG-----QKKGVVVKTILPDGVADSDGRLRAGDFILQINEHWLQGVGSEQVAAVLRGTGNVVKL 330 HSP 7 Score: 76.2554 bits (186), Expect = 9.655e-14 Identity = 60/197 (30.46%), Postives = 95/197 (48.22%), Query Frame = 0
Query: 153 KPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
KP G LG S+VG G I++ E+ KG A +DG+L GDQIL + L N++H A++ L++ +V LV+ R ED E ++ +VE+I+ + G G ++ + GV + VV+ G AD DGRL GD I+ +N + L E+ A +L+ + + + V R
Sbjct: 1653 KPMG--LGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLRPGDQILEVMNDNL-RNVTHSYALHALRQTPNKVRLVIHR---EDDEIYET---------------------FEVELIKRKDRG--LGLSIVGKKSGPGVYISEVVKGGAADSDGRLVQGDQIILVNGNDLTNSSQEEAAPILKMAQGKITMKVRR 1818 HSP 8 Score: 61.6178 bits (148), Expect = 3.027e-9 Identity = 104/455 (22.86%), Postives = 189/455 (41.54%), Query Frame = 0
Query: 478 QGLGITIAGYTCE--------------REELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR-YVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATP-LARSSPQGVDNEVDNKGESGGDSE-------LSESSESLQD---LENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLP-MTVKLVCARSQSQSTSVGTKQDFPLGGT-FRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKP 904
+GLGI+I G + +SGIF+K+V +GS A ++G + D+I+EVDG ++ S+++AV+++R + V R V G A + N + P + + A P A +S + E +++ +E + ++S S D ++++ E + + + + G L + ++ K GLGISL G ++ +I ++P G +G +K GD +LEVNG+ L H + +++K+ P V V + + K PL F H +L + + K AG L +I + K ++GLG I++ + + T + + L P + + G L+ GD ++ VN + AS + A + LK G++ + V P
Sbjct: 1172 KGLGISIVGGKVDPPSSDDGDSSSGSLNASVSGIFIKNVLEGSPAGQTGQLCTGDRILEVDGNDLRTASHERAVDVIRQSNNPVEEEEERDMVEG-------DANQGEEGNDNISMP---KDKIEAPPEFANTSTDKEEEEEESEEGELSGTETLSNGFVIDKASASFLDKSSIDDEEEDDYRYTMDKIRRKYGKFPGKL-SLVRLNKGIXGLGISLAGHKDR-----NQMAVFICGLIPQGNAARDGRIKIGDSVLEVNGKVLHNRCHLNASSLIKNFPDSAVTFVLLTPEEGGSQCAVK---PLSANQFPHGLLNKNNPIERYK-----KYAG-------------------------------LRQIRIKKEDQGLGIMIIEGKH--TEAGTGVFVSDLQPDSAADKAGLLV-GDMILCVNGEDFVGASYESAARVLKSLD-GIITMCVANP 1567 HSP 9 Score: 61.2326 bits (147), Expect = 3.376e-9 Identity = 35/91 (38.46%), Postives = 54/91 (59.34%), Query Frame = 0
Query: 2113 ATGGTMKTVELKRGQ-HGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLE-GLSHHDAVNILKNCDGAVMLQI 2201
A G +K ++L + + H LGFS+VG G++ IY++ + G A G L GDQIL+++ K L+ +SH A+NIL+ DG V L +
Sbjct: 142 AQGREIKRIKLYKPEGHSLGFSVVGLRSEFKGELGIYLQEIQPDGLAGKDGRLIEGDQILAIDGKSLDSNISHQQAINILQRADGYVDLVV 232 HSP 10 Score: 54.299 bits (129), Expect = 4.176e-7 Identity = 89/433 (20.55%), Postives = 180/433 (41.57%), Query Frame = 0
Query: 686 IRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDT-LLEIELIKGERGLGFSILDYQDPMNKDE-TVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALK--GAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDI--------QDEGINGL------------------VVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILR 1088
++++LP+G ++G L++GD +L++N L G+ V A+L+ VKL+ AR + S + + P+ L N E + + A T E+ + + ++ P + + + L++ELIK +GLG +I Y ++E + I ++S+ G + + G + D+++ V+ +++ + +AV+ L+ G + I L ++ + + S+TP +S ++SP ++ P + +A ++ L + ++ EG++ P +ER I ++K SD +G+Q +I Q G G+ +RSV G + +G + GD L+ VNG+ + H+ + IL+
Sbjct: 280 VKTILPDGVADSDGRLRAGDFILQINEHWLQGVGSEQVAAVLRGTGNVVKLIVARPIT-SXNKDXSRSLPVLPILS---LENREELEAHLQKTTSVGASTREEAPNIDLNQLISHQTTTPSAGVELMPEMETLDVELIKDSQGLGITIAGYT--CEREELSGIFVKSVTDGSAASRSGMVAVNDQIVEVDGNSIQGYTNQQAVEMLRSTGKSVKLKLIRYVHGLKFEQLQQ------AIANSQSNTPI------TSPTQMIQLSPHIS--------------------PTKSLHIEAIESKNQLNNDEVNENAVYEINNRVDSFEGELDP----IIEREI-IQKWSDYMGVQYEIVVAQLKKFQSTGGLGISLEGTVEKVDGEEHNPHHYIRSVLPDGPVGHNGLLKSGDELLEVNGQKLIGLYHTDVVSILK 669
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000001281 (supercontig:LSalAtl2s:LSalAtl2s1211:83672:134357:1 gene:EMLSAG00000001281 transcript:EMLSAT00000001281 description:"augustus_masked-LSalAtl2s1211-processed-gene-1.0") HSP 1 Score: 262.692 bits (670), Expect = 5.994e-72 Identity = 180/453 (39.74%), Postives = 255/453 (56.29%), Query Frame = 0
Query: 676 DGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR------SQSQSTSVG------TKQDFP----LGGTFRHEILANTE---RMVKAKSDVSISSAGTVTESSHQPQQS----------------KLKSRSLEPLTSL-AMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEP--IHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSR-DGRIYPGDYLVAVNGEVMRKASHSQALDILRR 1089
DG E PHHYIRSVLP GP+G +G L+SGDELLEVNG+ L GL+H +VV LK+LP+ V L+CAR S + T VG +K+ F L G+ + +LA E ++KAKS+VS++ + + S H+ + S + ++RSL+PLT AMWS ++ EI L+K E+GLGFSILDYQDPMN ETVIV+RSLVPGGV+Q D R++PGDR+M+VND LEH+SLD AVQALKGA G VRIG+++PLPL S ED S S N +V + R S S DG+ + +T L + + S RE P + I+ LP + + + ++ LG++++ + G VVR+V GG +++ + ++ PGD ++++N E + + SQA IL+R
Sbjct: 507 DGREVRPHHYIRSVLPYGPIGKHGGLRSGDELLEVNGKSLKGLFHEEVVKTLKELPLNVCLICARKIFNGPSPIRGTIVGNVDTGRSKKAFQSRKILSGSLQ-SLLALKEPELGLMKAKSEVSLAYTTSTSLSVHRGRNSDSRTPSSSAGSHLIGKRQRARSLDPLTDKPAMWSKSVEEIVLVKNEKGLGFSILDYQDPMNSSETVIVVRSLVPGGVAQTDCRVVPGDRIMFVNDTQLEHSSLDTAVQALKGASLGPVRIGISRPLPLEHSSST--EDKSPMNRSEGVKIGNEDVGA----LYRSSLSTY-----------------------DGESEIKTEFGCLYSI-YFIRSCTIKKRELPESLRGYPILRHLPKY--QVSLKKSKNEPLGLELEALAFELCGTVVRAVDGGGAVAKSEYQLRPGDLILSLNNESLWRVRSSQAKLILQR 926 HSP 2 Score: 134.42 bits (337), Expect = 1.273e-31 Identity = 56/104 (53.85%), Postives = 82/104 (78.85%), Query Frame = 0
Query: 463 MPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQA 566
+PE E +EL KD+ GLGIT+AGY CE+E+L GIFVKSV +GS A +SG ++VNDQ++EVDG S+ G++N +AVE+L+ T V+L+++RY+ GLKFE+ ++
Sbjct: 291 LPEKEEYSIELTKDSNGLGITVAGYICEKEDLCGIFVKSVIEGSMADRSGCIMVNDQVVEVDGISLAGYTNHEAVEILKQTASIVKLKIIRYLRGLKFEEFHES 394 HSP 3 Score: 105.916 bits (263), Expect = 8.311e-23 Identity = 54/124 (43.55%), Postives = 81/124 (65.32%), Query Frame = 0
Query: 260 VLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSG-NHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQ 382
VL DW Q++VI LTN+G GFGFG+ +G + +G VV+ + AD+DG L GD++L + D L G G EQVA++LR++G + +RL+VAR +DPS Q + I+P+ +L ELE ++
Sbjct: 2 VLQCDWAQIQVIVLTNDGNGFGFGI-SGSRNSGAVVRNIFPGCAADRDGSLQNGDHLLAVGDVRLWGMGAEQVASILRQAGQDSIRLVVARPIDPS-IPQFAINSTIIPTKVLTDPVELERTIE 123
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000004674 (supercontig:LSalAtl2s:LSalAtl2s244:169931:173253:1 gene:EMLSAG00000004674 transcript:EMLSAT00000004674 description:"snap_masked-LSalAtl2s244-processed-gene-1.7") HSP 1 Score: 99.7525 bits (247), Expect = 2.851e-21 Identity = 73/238 (30.67%), Postives = 108/238 (45.38%), Query Frame = 0
Query: 2016 GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILK-----------------MTQGK-------------------------IAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKR--------GQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNC-DGAVMLQIL 2202
G+ +S ++S G IS + G A DGRL GD+II +N H L EDA +L+ M+ G +A R R + N + + DS +P GG + LK+ G+ GLGFS+VGG SP G M I+VK++F+ G AA G L+ GD+ILS+N K L GLSH + ++ ++ +G +++ IL
Sbjct: 384 GISISAFSEESRHGFIISSIDDDGIAGKDGRLRIGDEIININDHSLRGIDIEDAEKLLRAGNLSEIDITVARYERVMSHGSSLLESSSTYSVDEGKRRPVRRKLPILADRPRSAPLFNEEASKTVIKVPDSVLPPTSKLKRGGRVGNQPLKKEITFEKGDGKRGLGFSVVGGKDSPRGSMGIFVKSIFENGQAAHEGSLREGDEILSINDKSLSGLSHQEVISEFRSVKNGKIVMSIL 621 HSP 2 Score: 59.6918 bits (143), Expect = 7.618e-9 Identity = 36/94 (38.30%), Postives = 56/94 (59.57%), Query Frame = 0
Query: 147 KHIALSKPEG-HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKAR-GRVHLVVARGSAED 238
K I K +G LGFSVVG K +G +GI+V+ I G AA +G L EGD+IL+I+ + L S +SH + I+ + + G++ + + R + D
Sbjct: 535 KEITFEKGDGKRGLGFSVVGGKDSPRGSMGIFVKSIFENGQAAHEGSLREGDEILSINDKSL-SGLSHQEVISEFRSVKNGKIVMSILRRNFND 627 HSP 3 Score: 55.8398 bits (133), Expect = 1.300e-7 Identity = 60/255 (23.53%), Postives = 114/255 (44.71%), Query Frame = 0
Query: 480 LGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGES-VRLRLVRYVHGLKF-EQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQ--DLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDL 730
LGI+I+ ++ E G + S+ D A K G + + D+II ++ S++G + A ++LR+ S + + + RY + L ++ + + P ++ + A RS+P + E DS L +S+ + + NQP + E + E + G G +++ K P G +GI +++S+ NG G L+ GDE+L +N + L GL H +V++ + +
Sbjct: 383 LGISISAFS--EESRHGFIISSIDDDGIAGKDGRLRIGDEIININDHSLRGIDIEDAEKLLRAGNLSEIDITVARYERVMSHGSSLLESSSTYSVDEGKRRPVRRKLPILAD-RPRSAPL-FNEEASKTVIKVPDSVLPPTSKLKRGGRVGNQP-LKKEITFEKGDGKRGLGFSVVGG---KDSPRGSMGI------------------FVKSIFENGQAAHEGSLREGDEILSINDKSLSGLSHQEVISEFRSV 611 HSP 4 Score: 53.5286 bits (127), Expect = 5.916e-7 Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 0
Query: 2015 RGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTN-SSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGV 2096
RGLG S+VG K P G+F+ + + G A +G L +GD+I+++N L+ S QE + + GKI M + R ++++
Sbjct: 546 RGLGFSVVGGKDSPRGSMGIFVKSIFENGQAAHEGSLREGDEILSINDKSLSGLSHQEVISEFRSVKNGKIVMSILRRNFNDKRRNC 632
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000005794 (supercontig:LSalAtl2s:LSalAtl2s311:59396:109515:-1 gene:EMLSAG00000005794 transcript:EMLSAT00000005794 description:"maker-LSalAtl2s311-augustus-gene-1.7") HSP 1 Score: 96.6709 bits (239), Expect = 3.501e-20 Identity = 75/252 (29.76%), Postives = 112/252 (44.44%), Query Frame = 0
Query: 2017 LGLSIVGKKS-------GPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQ-----------------------------GKIAMRVRR--------------------------LRVG--NRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRG--QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCD-GAVMLQI 2201
LG+ I KK+ G I+ + + G DGR GD+II VNG L + ++A L+ + G + R RR +++G N V H S IP P + ++KT+E ++G + GLGFS+VGG SP G M I+VKT+F G A +HG LK GD+IL+VN L+G+SH +A+++ KN G ++L +
Sbjct: 483 LGILIAKKKTIHMEKGATSGYIIAHIEESGIVHRDGRFKIGDEIINVNGKSLRGLNMDEAKETLRNSGHVVDIILGRDDNPDQDHFSFLSTPSSIPIGPVERRRRRKLPVVEPRPHSAPLEEPIEDNGTKTVIKIGAENSNIEVVRKHVSKSFIPRR-PKSLSMSIKTIEFEKGPGKKGLGFSVVGGIDSPKGSMGIFVKTIFPIGQAIEHGLLKEGDEILAVNGLALQGMSHSEAISVFKNIRIGKIVLFV 733 HSP 2 Score: 75.8702 bits (185), Expect = 8.590e-14 Identity = 82/306 (26.80%), Postives = 133/306 (43.46%), Query Frame = 0
Query: 1701 SGKYKGLHEVSILKGEQGLGIMIIEGK--HADVG--SGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGA--IFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTP-NRVRLMIHRED 1998
S K+K L + + LGI+I + K H + G SG ++ ++ G + GD I+ VNG+ G+N + A L+NS ++ +I+ P D S ++ P S +P P E + KLP + P+P H P+ P G K + ++ + +E + ++ K R K+ S E PG GLG S+VGG D+ G+ IF+ ++ G A + G LK GD+IL V L+ +SHS A+ + ++ L + R D
Sbjct: 466 SKKFKMLR-LRKVDASTDLGILIAKKKTIHMEKGATSGYIIAHIEESGIVHRDGRFKIGDEIINVNGKSLRGLNMDEAKETLRNSGHVVDIILGRDDNP-DQDHFSFLSTPSS------IPIGP-VERRRRRKLP---VVEPRP------HSAPLEEPIEDNGTKTVIKIGAENSNIEVVRKHVSKSFIPRRPKSLSMSIKTIEFEKGPGKK----------------GLGFSVVGGIDSPKGSMGIFVKTIFPIGQAIEHGLLKEGDEILAVNGLALQGMSHSEAISVFKNIRIGKIVLFVARRD 737 HSP 3 Score: 67.0106 bits (162), Expect = 4.832e-11 Identity = 65/312 (20.83%), Postives = 135/312 (43.27%), Query Frame = 0
Query: 245 PPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQ---------AAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPIL--PSSMLAKRAELETHLQIAVVANSSFVSAASHGPP---PLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRS 542
PP+ + P S+ + N + +++ L A G++ ++ +G ++ + ++G+ +DGR GD I+ +N L+G +++ LR SG+ V +I+ R+ +P + H L PSS+ E ++ V V H P P+ + ++ E +I + K V ++ P + + L + ++T++ E +GLG ++ G + GIFVK++ A + G++ D+I+ V+G ++QG S+ +A+ + ++
Sbjct: 446 PPQLQSAPMSSSSSKTNNLTSKKFKMLRLRKVDASTDLGILIAKKKTIHMEKGATSGYIIAHIEESGIVHRDGRFKIGDEIINVNGKSLRGLNMDEAKETLRNSGHVVDIILGRDDNPD-----QDHFSFLSTPSSIPIGPVERRRRRKLPV------VEPRPHSAPLEEPIEDNGTKTVIKIGAENSNI---EVVRKHVSKSFIPRRPKSLSMS--------------------IKTIEFEKGPGKKGLGFSVVGGIDSPKGSMGIFVKTIFPIGQAIEHGLLKEGDEILAVNGLALQGMSHSEAISVFKN 723 HSP 4 Score: 65.4698 bits (158), Expect = 1.534e-10 Identity = 40/90 (44.44%), Postives = 55/90 (61.11%), Query Frame = 0
Query: 146 VKHIALSK-PEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKAR-GRVHLVVAR 233
+K I K P LGFSVVG KG +GI+V+ I P G A + G L EGD+ILA++G L +SH +AI++ + R G++ L VAR
Sbjct: 647 IKTIEFEKGPGKKGLGFSVVGGIDSPKGSMGIFVKTIFPIGQAIEHGLLKEGDEILAVNGLALQG-MSHSEAISVFKNIRIGKIVLFVAR 735 HSP 5 Score: 56.225 bits (134), Expect = 9.378e-8 Identity = 64/235 (27.23%), Postives = 105/235 (44.68%), Query Frame = 0
Query: 684 HYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR----SQSQSTSVGTKQDFPLG---GTFRHEILANTERMVKAKSDVSISSAGTVT------ESSHQPQQSKLKSRSLEPL--TSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTK 903
+ I + +G V +G K GDE++ VNG+ L GL + L++ V ++ R Q + + T P+G R ++ R A + I GT T E+S+ K S+S P SL+M T +E E G++GLGFS++ D K I ++++ P G + + G L GD ++ VN + L+ S EA+ K G + + V +
Sbjct: 503 YIIAHIEESGIVHRDGRFKIGDEIINVNGKSLRGLNMDEAKETLRNSGHVVDIILGRDDNPDQDHFSFLSTPSSIPIGPVERRRRRKLPVVEPRPHSAPLEEPIEDNGTKTVIKIGAENSNIEVVRKHVSKSFIPRRPKSLSMSIKT-IEFEKGPGKKGLGFSVVGGIDSP-KGSMGIFVKTIFPIGQAIEHGLLKEGDEILAVNGLALQGMSHSEAISVFKNIRIGKIVLFVAR 735
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000003541 (supercontig:LSalAtl2s:LSalAtl2s1945:30586:44555:1 gene:EMLSAG00000003541 transcript:EMLSAT00000003541 description:"maker-LSalAtl2s1945-augustus-gene-0.11") HSP 1 Score: 90.1225 bits (222), Expect = 4.900e-18 Identity = 123/500 (24.60%), Postives = 202/500 (40.40%), Query Frame = 0
Query: 1346 ERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPV--VTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDP-----------VPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIY--VSLNKGTNGLGISLAGHKDRSKM-------SVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVK--PLSQFPPEPHKDNPIERYSGKYKGLHEVSILK--GEQGLGIMIIEGKHADVGS-------GVFVSDLQPGSCADV-AGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPA 1811
E+ R ++GLG+SI GGK G +P + +FI + E PA + L+ GDR+L ++GH++ A H + V +++ V+ +V+ + VT+ S S S+ SE P P L S AN P T S +++PG SSS S+ D +S + + + L+ A+ P + E + F +T +K+ P+ V ++K LG+ + G D+S + ++I + G A R ++++GD IL V+G+ I+D H + + PD N+ VK PL Q G E+ I K GE+ LG+ I G G+ G+F+ PGS A L G I+ VN +G+ + A I++N++ I+M+V + + S I +P PP
Sbjct: 179 EKISTTLRRDHRGLGLSIAGGK------------GADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDRYVQLLVERITQVTSPMS----SSVLSNTSEKSPKVFGLPKPYTGLY-SASSYMANRPSYMRTREPGQYTITGSSFSGSSSYSKLPG------LSSSYKSESDVSIES--KLKEKNIVMXLIXKLPPASKEPGVSTETMKRTTFTETT---VKRVTNNDIKPLIEEVIISKAGGRLGLCIIGGSDQSSVPFGNGEPGIFISNIIADGXAARTNKLRLGDRILNVHGNDIHDASHHDAVMALSQTPDQ-------------LNITVKHEPLPQ-------------------GFQEICITKDPGEK-LGMTIRGGVGGQPGNPNDGDDEGIFIYKFTPGSVASQNKDLVIGQRIIEVNALSLLGITHSEALNIIRNAKSSIRMVVCDGWNVPEKVSNGDIKSPPPPPT 617 HSP 2 Score: 78.5666 bits (192), Expect = 1.564e-14 Identity = 107/418 (25.60%), Postives = 173/418 (41.39%), Query Frame = 0
Query: 1710 VSILKGEQGLGIMIIEGKHAD---VGS-GVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVV----PTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVE---SSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENG--KPM-----------GLGLSIVGGSDTL---LG----AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI-HREDDEIYETLDVELNKKKDRGLGLSIVGKKSG----------PGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRV 2084
++ + +GLG+ I GK AD GS VF+S + A + G L GD +L +NG + + R+LK + ++++V I V S S++ + S +P V P T ++ P +PG + I + K G+ +S + + SK + V K S P T T E + + N KP+ LGL I+GGSD G IFI + G A + +L+ GD+IL V D+ + SH A+ AL QTP+++ + + H + ++ ++ + K LG++I G G G+FI + G A + LV G +II VN L + +A I++ + I M V
Sbjct: 183 TTLRRDHRGLGLSIAGGKGADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDRYVQLLVER-ITQVTSPMSSSVLSNTSEKSPKVFGLPKPYTGLYSASSYMANRPSYMRTREPGQYT---ITGSSFSGSSSYSKLPGLSSSYKSESDVSIESKLKEKNIVMXLIXKLPPASKEPGVSTETMKRTTFTETTVKRVTNNDIKPLIEEVIISKAGGRLGLCIIGGSDQSSVPFGNGEPGIFISNIIADGXAARTNKLRLGDRILNVHGNDIHDASHHDAVMALSQTPDQLNITVKHEPLPQGFQ--EICITKDPGEKLGMTIRGGVGGQPGNPNDGDDEGIFIYKFTPGSVASQNKDLVIGQRIIEVNALSLLGITHSEALNIIRNAKSSIRMVV 594 HSP 3 Score: 66.2402 bits (160), Expect = 8.956e-11 Identity = 80/292 (27.40%), Postives = 126/292 (43.15%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGA---------IFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEV-VKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMK-TVELKRGQHGLGFSIVGGFGS-PH--GDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
GLGLSI GG LG+ IFI V G A G LK D++L V ++ H ++ L+ + + + I RE V++ KK+ FI ++ ++ +D D + VN L+N SQ + +K ++ ++ V + + + H DSSI N P T K + L+R GLG SI GG G+ P+ G +++ + + G AA G L GD++L +N +E H + V +LK D V L +
Sbjct: 14 GLGLSIAGG----LGSTPYKANDEGIFISRVTPGGPAELAG-LKKDDKVLSVNGHSCVDIDHYESVGILKAAGSLITMKIARE---------VQVPKKR-----------------FIDQLPLQNNVSDHLSSKNGLDNTLEVNSSTLSNFSQSE----IKESK---SLSVSTTGLTPKSSQLSHTHLFDSSI-VNSPTETRKIEKISTTLRRDHRGLGLSIAGGKGADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDRYVQLLV 266 HSP 4 Score: 62.3882 bits (150), Expect = 1.241e-9 Identity = 51/201 (25.37%), Postives = 86/201 (42.79%), Query Frame = 0
Query: 2017 LGLSIVGKKSG---------PGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHG------DMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
LGL I+G PG+FIS ++ G A +L GD+I+ V+G+D+ ++S DA L T ++ + V+ +P G + K LG +I GG G G D I++ A+ + L G +I+ VNA L G++H +A+NI++N ++ + +
Sbjct: 419 LGLCIIGGSDQSSVPFGNGEPGIFISNIIADGXAARTNKLRLGDRILNVHGNDIHDASHHDAVMALSQTPDQLNITVKH-----------------EPLPQ-------GFQEICITKDPGEKLGMTIRGGVGGQPGNPNDGDDEGIFIYKFTPGSVASQNKDLVIGQRIIEVNALSLLGITHSEALNIIRNAKSSIRMVVC 595 HSP 5 Score: 60.4622 bits (145), Expect = 4.880e-9 Identity = 66/249 (26.51%), Postives = 109/249 (43.78%), Query Frame = 0
Query: 663 GLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEI-----LANTERMVKA------KSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTL----LEIELIKGERGLGFSILDYQ--DPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPR 894
GLG+S+ G + + + +I V P GP G LK D++L VNG + + H + V ILK + + AR + Q PL + L NT + + +S++ S + +V+ + P+ S+L L + + ++T + L + RGLG SI + DP + + I + G + DG+L GDRL+ +N +E+A E V+ LKG R
Sbjct: 14 GLGLSIAGGLGSTPYKANDEGIFISRVTPGGPAELAG-LKKDDKVLSVNGHSCVDIDHYESVGILKAAGSLITMKIAR-EVQVPKKRFIDQLPLQNNVSDHLSSKNGLDNTLEVNSSTLSNFSQSEIKESKSLSVSTTGLTPKSSQLSHTHLFDSSIVNSPTETRKIEKISTTLRRDHRGLGLSIAGGKGADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDR 260 HSP 6 Score: 58.9214 bits (141), Expect = 1.502e-8 Identity = 68/277 (24.55%), Postives = 130/277 (46.93%), Query Frame = 0
Query: 479 GLGITIAG---YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQA-IANSQSNSQATTPSV-SQVAVSATPLARSSPQGVDNE----VDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHH------YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR 740
GLG++IAG T + GIF+ VT G A +G+ +D+++ V+G S + ++V +L++ G + +++ R V K + Q + N+ S+ ++ + + + V+++ L+ S + V G + S+LS + + N P +ET + I + ++ + GLG+S+ G G+ +P+ +I + NGP +G L GD LL++NG ++ H++VV +LK V+L+ R
Sbjct: 14 GLGLSIAGGLGSTPYKANDEGIFISRVTPGGPAELAGLKK-DDKVLSVNGHSCVDIDHYESVGILKAAGSLITMKIAREVQVPKKRFIDQLPLQNNVSDHLSSKNGLDNTLEVNSSTLSNFSQSEIKESKSLSVSTTGLTPKSSQLSHTHLFDSSIVNSP---TETRK------------IEKISTTLRRDHRGLGLSIAG------GKGADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDRYVQLLVER 268 HSP 7 Score: 55.0694 bits (131), Expect = 2.300e-7 Identity = 36/122 (29.51%), Postives = 62/122 (50.82%), Query Frame = 0
Query: 1974 VSHSHAL-HALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSG-------PGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRL 2087
+SH+H ++ +P R + ++I TL ++ RGLGLSI G K VFIS++ + G A DG+L GD+++ +NGH++ N+ + +LK + + V R+
Sbjct: 158 LSHTHLFDSSIVNSPTETRKI-----EKISTTL-----RRDHRGLGLSIAGGKGADPYIQGSESVFISKIAENGPAAIDGKLYVGDRLLQINGHEVENAEHSEVVRMLKGPDRYVQLLVERI 269
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000009687 (supercontig:LSalAtl2s:LSalAtl2s623:567590:593389:1 gene:EMLSAG00000009687 transcript:EMLSAT00000009687 description:"maker-LSalAtl2s623-augustus-gene-5.6") HSP 1 Score: 88.5817 bits (218), Expect = 1.622e-17 Identity = 108/406 (26.60%), Postives = 184/406 (45.32%), Query Frame = 0
Query: 159 LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKA--RGRVHLVVARGS---AEDSESTQSPPETTA---LPSRESTPRVLNSDW---CQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKS--GNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHL--PKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCERE----ELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLR 541
LG VV E G L VQ I+P G ++G+L GD+++ I+G L +++S ++A +I +KA + L V +G E+S++ ++ E +P + T V ++ +V I LT AG GF + A G + +K V+ G A ++G L GD +L++N + G G +V ++LR + +++ L+V+R +D + ++ + PI +S L I+ ++ PPP T + P + + PS P S D+ +E L D+P +T + GLG+++ G T + +L GIFVKSV G AA + G + NDQ++ ++G + G A+E LR
Sbjct: 316 LGIHVV--PCEMDGRL--VVQGIEPGGRVDRNGRLAVGDEVVDINGCCL-TDVSFNKAQDIFKKALLDHELRLRVIKGGEFYQENSDNKENISEKKIGDLVPGTKITSAVNANNTRKIGKVMKITLTKGAAGLGFSITGRDNAPGGTTPIYIKNVLPKGAAIEEGNLKPGDRLLEVNSCRVDGMGQSEVVSLLRSAPLXSNLELVVSRHLD-TTSNTISQTTPIHTNS-----------LNISTSYGHEMEDNSTQVPPPSTLITQ----PKMPKDFKSHSPSPHGGPISADEFPWKHREILTF------------------DIPVHDT-------ERAGLGVSVKGKTSTGKAGIIDL-GIFVKSVLHGGAASRDGRLRTNDQLVNINGLXLLGKPKPXAMETLR 674 HSP 2 Score: 87.4261 bits (215), Expect = 4.138e-17 Identity = 68/219 (31.05%), Postives = 109/219 (49.77%), Query Frame = 0
Query: 1993 MIHREDDEIYETLD--VELNKKKDRG-LGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVG-------NRKQGVGGMHSRD--------SSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNC 2193
M RE E+ E L VE+ + G LG+ +V + + + + GG D +GRL GD+++ +NG LT+ S A I K +R+R ++ G + K+ + D S++ +N G MK + L +G GLGFSI G +P G PIY+K V GAA + G LK GD++L VN+ ++G+ + V++L++
Sbjct: 289 MGSRESRELKERLCGVVEVVIPNEVGPLGIHVVPCEMDGRLVVQGIEPGGRVDRNGRLAVGDEVVDINGCCLTDVSFNKAQDIFKKALLDHELRLRVIKGGEFYQENSDNKENISEKKIGDLVPGTKITSAVNANNTRKIGKVMK-ITLTKGAAGLGFSITGRDNAPGGTTPIYIKNVLPKGAAIEEGNLKPGDRLLEVNSCRVDGMGQSEVVSLLRSA 506 HSP 3 Score: 82.0333 bits (201), Expect = 1.519e-15 Identity = 108/430 (25.12%), Postives = 176/430 (40.93%), Query Frame = 0
Query: 686 IRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKD--LPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAP-RGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLP-TSPPPEDGDFDARTAERLLGTTSKGVISAR---FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNG------------EVMRKASHSQ-------ALDILRR 1089
++ + P G V NG L GDE++++NG L + I K L ++L + GG F E N E + + K + GT S+ ++ + +++I L KG GLGFSI +D T I I++++P G + ++G L PGDRL+ VN ++ E V L+ AP + + V++ L D +++T S +TP +N S M +D+ T +PPP + P DF + + G S + ++ + P+H+ + A L ++ + + GI +D+ G+ V+SV GG SRDGR+ D LV +NG E +RKA H + +L I RR
Sbjct: 331 VQGIEPGGRVDRNGRLAVGDEVVDINGCCLTDVSFNKAQDIFKKALLDHELRLRVIK----------------GGEFYQENSDNKENISEKK--IGDLVPGTKITSAVNANNTRKIGK--------------VMKITLTKGAAGLGFSITG-RDNAPGGTTPIYIKNVLPKGAAIEEGNLKPGDRLLEVNSCRVDGMGQSEVVSLLRSAPLXSNLELVVSRHL-----------DTTSNTISQTTPIHTNSLNISTSYGHEM----------EDNSTQVPPPSTLITQPKMPKDFKSHSPSPHGGPISADEFPWKHREILTFDIPVHDTE-----RAGLGVSVKGKTSTGKAGI-IDL------GIFVKSVLHGGAASRDGRLRTNDQLVNINGLXLLGKPKPXAMETLRKAMHEEGPVPGIISLTIARR 694 HSP 4 Score: 56.225 bits (134), Expect = 1.126e-7 Identity = 61/227 (26.87%), Postives = 107/227 (47.14%), Query Frame = 0
Query: 685 YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPM--TVKLVCAR-------SQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLE--PLTSLAM-WSDT---LLEIELIKGER-GLGFSILDYQDPMNKDETV---IVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGA 892
YI++VLP G G LK GD LLEVN ++ G+ ++VV++L+ P+ ++LV +R + SQ+T + T + ++ HE+ N+ + T+ P+ K S S P+++ W +I + ER GLG S+ + K + I ++S++ GG + +DGRL D+L+ +N + L A++ L+ A
Sbjct: 461 YIKNVLPKGAAIEEGNLKPGDRLLEVNSCRVDGMGQSEVVSLLRSAPLXSNLELVVSRHLDTTSNTISQTTPIHT-NSLNISTSYGHEMEDNS---------TQVPPPSTLITQPKMPKDFKSHSPSPHGGPISADEFPWKHREILTFDIPVHDTERAGLGVSVKG-KTSTGKAGIIDLGIFVKSVLHGGAASRDGRLRTNDQLVNINGLXLLGKPKPXAMETLRKA 676
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000001217 (supercontig:LSalAtl2s:LSalAtl2s11:167499:170412:-1 gene:EMLSAG00000001217 transcript:EMLSAT00000001217 description:"maker-LSalAtl2s11-augustus-gene-1.17") HSP 1 Score: 77.0258 bits (188), Expect = 4.335e-14 Identity = 44/95 (46.32%), Postives = 61/95 (64.21%), Query Frame = 0
Query: 2112 GATGG--TMKTVELKRG--QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCD-GAVMLQI 2201
G TG +T+ ++G + LGFSIVGG SP G M I+VKT+ TG +A+ G L+ GD+IL+VN + L GL+H +A+ I K G V+LQI
Sbjct: 683 GTTGSPQIYRTIVYQKGPGKKSLGFSIVGGRDSPKGSMGIFVKTILPTGQSAEDGRLQEGDEILAVNGEILHGLNHGEAIAIFKRIKSGPVVLQI 777 HSP 2 Score: 67.0106 bits (162), Expect = 5.118e-11 Identity = 49/164 (29.88%), Postives = 82/164 (50.00%), Query Frame = 0
Query: 75 HPSILPSDFDIGV----NGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKAR-GRVHLVVAR 233
H +LP+ + N LP + S + P+ + PSP+ T + ++ ++ Q G+ SLGFS+VG + KG +GI+V+ I P G +A+DG+L EGD+ILA++G+ L ++H +AI I ++ + G V L + R
Sbjct: 629 HEEVLPTVIRVNCRQLPNQPNFCTLPRKDTILSGSNRKLPSIVTSPSPVSTTSIGTTGSPQIYRTIVYQKGPGK------------KSLGFSIVGGRDSPKGSMGIFVKTILPTGQSAEDGRLQEGDEILAVNGEILHG-LNHGEAIAIFKRIKSGPVVLQIGR 779 HSP 3 Score: 58.9214 bits (141), Expect = 1.556e-8 Identity = 65/293 (22.18%), Postives = 135/293 (46.08%), Query Frame = 0
Query: 260 VLNSDWCQVEVIELTNNGAGFGFGVMAGQQ--AAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLR-KSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGT--------TARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLR 541
L ++ V ++ + G M Q+ G + + +G+ +DGRL GD I+ +N +G +++ +L K+ + V +++AR + PS S+ +I P S+LA E + ++ +S P L + +++ +LPT +V+ Q LP + T P ++ +L G+ T+ S + ++ +G + G T+ + + LG +I G + GIFVK++ + + G + D+I+ V+G+ + G ++ +A+ + +
Sbjct: 494 TLKKEFRLVRLLNVEQRTLGIFIARMEQQEVGCIGYYIAHINFDGIVHRDGRLMIGDEIVNVNGRRXRGLPMDEAKALLNYKNDSSVDIVIARVITPSSTSKYS-NISNSPLSLLANHNEED------------LINLSSR--PLLKSSSHEEVLPTVI-RVNCRQ---LPNQPNFCTLPRKDTILSGSNRKLPSIVTSPSPVSTTSIGTT-GSPQIYRTIVYQKGPGKKSLGFSIVGGRDSPKGSMGIFVKTILPTGQSAEDGRLQEGDEILAVNGEILHGLNHGEAIAIFK 766 HSP 4 Score: 55.4546 bits (132), Expect = 1.802e-7 Identity = 67/272 (24.63%), Postives = 118/272 (43.38%), Query Frame = 0
Query: 476 DAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGES-VRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSP-------------QGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGS-GHAILMATIQKQKPNG--GLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDL 730
+ + LGI IA + G ++ + + G +++ D+I+ V+G+ +G +A +L +S V + + R + + I+NS + A + +S+ PL +SS + + N+ + D+ LS S+ L + P S TS +G+ G + TI QK G LG S+ G + G +++++LP G +G L+ GDE+L VNG L GL H + +AI K +
Sbjct: 508 EQRTLGIFIARMEQQEVGCIGYYIAHINFDGIVHRDGRLMIGDEIVNVNGRRXRGLPMDEAKALLNYKNDSSVDIVIARVITPSSTSKYSN-ISNSPLSLLANHNEEDLINLSSRPLLKSSSHEEVLPTVIRVNCRQLPNQPNFCTLPRKDTILSGSNRKLPSIVTSPSPVSTTS-------IGTTGSPQIYRTIVYQKGPGKKSLGFSIVGGRDSPKGSMG---IFVKTILPTGQSAEDGRLQEGDEILAVNGEILHGLNHGEAIAIFKRI 768
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000006760 (supercontig:LSalAtl2s:LSalAtl2s378:96640:99039:1 gene:EMLSAG00000006760 transcript:EMLSAT00000006760 description:"maker-LSalAtl2s378-augustus-gene-1.12") HSP 1 Score: 71.633 bits (174), Expect = 6.269e-14 Identity = 37/83 (44.58%), Postives = 52/83 (62.65%), Query Frame = 0
Query: 2119 KTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
+ VEL + + GLGF+++GG + PIY+ + G A HGGLKRGDQ+LSVN +EG H AV++LK G+V L +
Sbjct: 96 RVVELPKTEEGLGFNVMGG---KEQNSPIYISRIIPGGVADRHGGLKRGDQLLSVNGVSVEGEHHEKAVDLLKAASGSVKLVV 175 HSP 2 Score: 54.6842 bits (130), Expect = 3.406e-8 Identity = 27/75 (36.00%), Postives = 44/75 (58.67%), Query Frame = 0
Query: 2012 KKDRGLGLSIVG-KKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVR 2085
K + GLG +++G K+ ++IS ++ GG AD G L +GDQ+++VNG + E A +LK G + + VR
Sbjct: 102 KTEEGLGFNVMGGKEQNSPIYISRIIPGGVADRHGGLKRGDQLLSVNGVSVEGEHHEKAVDLLKAASGSVKLVVR 176 HSP 3 Score: 53.1434 bits (126), Expect = 1.233e-7 Identity = 31/80 (38.75%), Postives = 50/80 (62.50%), Query Frame = 0
Query: 1710 VSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADV-AGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIV 1788
V + K E+GLG ++ GK + S +++S + PG AD GL+RGD +L+VNG G ++E A +LK + G +K++V
Sbjct: 98 VELPKTEEGLGFNVMGGKEQN--SPIYISRIIPGGVADRHGGLKRGDQLLSVNGVSVEGEHHEKAVDLLKAASGSVKLVV 175
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000007601 (supercontig:LSalAtl2s:LSalAtl2s43:145488:158360:1 gene:EMLSAG00000007601 transcript:EMLSAT00000007601 description:"maker-LSalAtl2s43-snap-gene-1.14") HSP 1 Score: 75.485 bits (184), Expect = 1.265e-13 Identity = 57/204 (27.94%), Postives = 93/204 (45.59%), Query Frame = 0
Query: 2017 LGLSIVG--KKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVG---------------------------NRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILK 2191
+G+++ G + + +V+ G AD DGR+ +GD+++++NG + +A ILK + ++ + + R R + K + + D +P + P TV LK+ GLGFS+ GG SP GD P+ +K +F GAA LK GD+ILSVN +S +A N +K
Sbjct: 652 IGITLAGGVDYENKDITVHKVIPGTLADRDGRIQKGDRVLSINGRSTKGVTHREALSILKGPRAEVVLVLSRSRSVTPAEYDRTDFSSYGSNGRPPKILESPLDHKSLLSDLKFVD--VPRSPPV-------TVVLKKEGTGLGFSLEGGKDSPVGDRPLTIKKIFTGGAADKSSILKIGDEILSVNNTDCTRMSRIEAWNFMK 846 HSP 2 Score: 63.929 bits (154), Expect = 5.056e-10 Identity = 69/276 (25.00%), Postives = 120/276 (43.48%), Query Frame = 0
Query: 648 HAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSL---EPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPL-------PLPEVSEQ 913
H I++ + ++ G +GI+L G V+ +N + V+P +G ++ GD +L +NGR G+ H + ++ILK V LV +RS+S + + + DF G+ +++++ D H+ S LK + P+T + L K GLGFS+ +D D + I+ + GG + + L GD ++ VN+ + S EA +K GM I V + L P P+V+E+
Sbjct: 636 HEIMVIVLHREFTAGSIGITLAGGVDY-----ENKDITVHKVIPGTLADRDGRIQKGDRVLSINGRSTKGVTHREALSILKGPRAEVVLVLSRSRSVTPAEYDRTDFSSYGSN-----GRPPKILESPLD-------------HKSLLSDLKFVDVPRSPPVT-----------VVLKKEGTGLGFSLEGGKDSPVGDRP-LTIKKIFTGGAADKSSILKIGDEILSVNNTDCTRMSRIEAWNFMKKLSDGMASIVVRQKLDNTEHSPPTPDVTEK 876 HSP 3 Score: 62.3882 bits (150), Expect = 1.507e-9 Identity = 60/234 (25.64%), Postives = 100/234 (42.74%), Query Frame = 0
Query: 1925 LGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHR-------EDDEI--------------------YETLDVELN--------------KKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGA 2113
+G+++ GG D I +H+V A +DGR++ GD++L + + V+H AL L+ V L++ R E D +++L +L KK+ GLG S+ G K P + I ++ GGAAD L GD+I++VN D T S+ +A +K +A V R ++ N + + S P+ +PG+
Sbjct: 652 IGITLAGGVDYENKDITVHKVIPGTLADRDGRIQKGDRVLSINGRSTKGVTHREALSILKGPRAEVVLVLSRSRSVTPAEYDRTDFSSYGSNGRPPKILESPLDHKSLLSDLKFVDVPRSPPVTVVLKKEGTGLGFSLEGGKDSPVGDRPLTIKKIFTGGAADKSSILKIGDEILSVNNTDCTRMSRIEAWNFMKKLSDGMASIVVRQKLDNTEHSPPTPDVTEKSNPNILPGS 885 HSP 4 Score: 62.003 bits (149), Expect = 1.586e-9 Identity = 55/215 (25.58%), Postives = 105/215 (48.84%), Query Frame = 0
Query: 158 SLGFSVVG-LKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARG-SAEDSESTQSPPETTALPSRESTPRVLN---------SDWCQVEV-------IELTNNGAGFGFGVMAGQQA----AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVARE 350
S+G ++ G + E+K I V ++ P +A +DG++ +GD++L+I+G+ ++H +A++IL+ R V LV++R S +E ++ + ++ S P++L SD V+V + L G G GF + G+ + + +K + G AD+ L GD IL +N+ + N ++K + + IV R+
Sbjct: 651 SIGITLAGGVDYENKD---ITVHKVIPGTLADRDGRIQKGDRVLSINGRST-KGVTHREALSILKGPRAEVVLVLSRSRSVTPAEYDRT--DFSSYGSNGRPPKILESPLDHKSLLSDLKFVDVPRSPPVTVVLKKEGTGLGFSLEGGKDSPVGDRPLTIKKIFTGGAADKSSILKIGDEILSVNNTDCTRMSRIEAWNFMKKLSDGMASIVVRQ 859
BLAST of conserved hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000006552 (supercontig:LSalAtl2s:LSalAtl2s359:307479:310647:1 gene:EMLSAG00000006552 transcript:EMLSAT00000006552 description:"maker-LSalAtl2s359-augustus-gene-3.5") HSP 1 Score: 70.0922 bits (170), Expect = 4.062e-12 Identity = 35/77 (45.45%), Postives = 50/77 (64.94%), Query Frame = 0
Query: 2117 TMKTVELKRG--QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILK 2191
T++TV ++G LGFSIVGG SP G M IYVKT+F TG A G L+ GD+I S+N+ + G +H + +++ K
Sbjct: 423 TIQTVTFEKGPGNKSLGFSIVGGKDSPKGSMGIYVKTIFPTGQA--QGVLQEGDEIYSINSNSVSGKTHQEVISLFK 497
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|317373392|sp|O75970.2|MPDZ_HUMAN (RecName: Full=Multiple PDZ domain protein; AltName: Full=Multi-PDZ domain protein 1) HSP 1 Score: 476.478 bits (1225), Expect = 1.161e-135 Identity = 321/914 (35.12%), Postives = 475/914 (51.97%), Query Frame = 0
Query: 1342 NWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGF---ANDPVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGK--------------YKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRED-----DEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVG----------NRKQGVGGMHS------------------RDSSIPSNVPGATGGTMKTVELKRG-QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
NW R V + R P++ LGISIVGG+ S N V+ GIFIK+VLE SPAG + L GDRI+ VDG DL A+H+QAV+ IR++G V F+VQS++ + P P P L+ ++ P + + +P + ++ + ++ + ++ S + + G Q++ S K + + ++EDEFGYS + I ++Y G + L KG +GLG+SLAG+KDRS+MSV+I G+ P+G A +D R+Q+ D +LE+NG ++Y R H N SS IK P S V I +R+ +++ +AV P + P P ++ + +K + + + K + GLGI I E D SGV + L A G L+ GD ILAV+ E VG E +LK ++ +K+ T P VP+ S +K K + + P+ G+ P I + T+ S+ A +S+PATC I PG +T IEISK + GLGLSIVGGSDTLLGAI IHEVYE+GAA KDGRL GDQILEV DLR +H A++ LRQTP RVRL ++R++ +E+ +TL +EL KK +GLGLSIVGK++ GVF+S++VKGG AD+DGRL+QGDQI+ VNG D+ N++QE A +LK + G + + V R++ G + + G + S + +++ S + G ++TVE+K+G LG SI GG GSP GD+PI++ + TG AA L+ GD+I+++ EG++H AVN+LKN G++ +Q+++
Sbjct: 1145 NWNQPRRVELWREPSKSLGISIVGGR-------GMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSII------------------------NRPRKSPLPSLLHNLYPKYNFSSTNPFADSLQINADKAPSQSESEPEKAPLCSVPPPPPSAFAEMGSDHTQSSAS--------------KISQDVDKEDEFGYSWKNIRERYGTLTGELHMIELEKGHSGLGLSLAGNKDRSRMSVFIVGIDPNGAAGKDGRLQIADELLEINGQILYGRSHQNASSIIKCAP-SKVKIIFIRNKDAVNQMAVCPGNAVEPLPSNSENLQNKETEPTVTTSDAAVDLSSFKNVQHLELPKDQGGLGIAISE---EDTLSGVIIKSLTEHGVAATDGRLKVGDQILAVDDEIVVGYPIEKFISLLKTAKMTVKL-----------------TIHAENPDSQAVPSAAGAASGEK-KNSSQSLMVPQSGSPEPESI--------------------RNTSRSSTPAIF--------------ASDPATCPIIPGCETTIEISKGR------TGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYRDEAPYKEEEVCDTLTIELQKKPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRNATQEAVAALLKCSLGTVTLEVGRIKAGPFHSERRPSQSSQVSEGSLSSFTFPLSGSSTSESLESSSKKNALASEIQG-----LRTVEMKKGPTDSLGISIAGGVGSPLGDVPIFIAMMHPTGVAAQTQKLRVGDRIVTICGTSTEGMTHTQAVNLLKNASGSIEMQVVA 1946 HSP 2 Score: 325.094 bits (832), Expect = 4.152e-88 Identity = 211/462 (45.67%), Postives = 272/462 (58.87%), Query Frame = 0
Query: 465 EMETLDVELVKDAQGLGITIAGYTCERE-ELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKG------ESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR------SQSQSTSVG------TKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPL 907
E ET DVEL K+ QGLGITIAGY +++ E SGIFVKS+T SA G + + DQII VDG ++QGF+NQQAVE+LR TG++V L L+R G+K Q+A S+ + T V+A+ + + + D + E G LS E ++D + Q W+ +G + I++A + K N GLGISLE TV HH+IRSVLP GPVG +G L SGDELLEVNG LLG H DVV ILK+LP+ V +VC R +QS+ S+ T++ G F + TE V A +D AG TE P LAMW + IEL KG +GLGFSILDYQDP++ TVI+IRSLVPGG++++DGRL+PGDRLM+VND+NLE++SL+EAV+ALKGAP G VRIGV KPLPL
Sbjct: 372 ESETFDVELTKNVQGLGITIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVLLTLMR--RGMK----QEAELMSRED---VTKDADLSPVNASIIKENYEKDEDFLSSTRNTNILPTEEEGYPLLSAEIEEIEDAQKQEAALLTK----WQRIMGINYEIVVAHVSKFSENSGLGISLEATV---------GHHFIRSVLPEGPVGHSGKLFSGDELLEVNGITLLGENHQDVVNILKELPIEVTMVCCRRTVPPTTQSELDSLDLCDIELTEKPHVDLGEFIGS--SETEDPVLAMTD-----AGQSTEEVQAP---------------LAMWEAGIQHIELEKGSKGLGFSILDYQDPIDPASTVIIIRSLVPGGIAEKDGRLLPGDRLMFVNDVNLENSSLEEAVEALKGAPSGTVRIGVAKPLPL 789 HSP 3 Score: 225.713 bits (574), Expect = 1.112e-57 Identity = 141/316 (44.62%), Postives = 191/316 (60.44%), Query Frame = 0
Query: 43 LATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIG----VNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEP---RFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAED--SESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
L+ L SVL+SP+F IL++Q S+ +LK QV + S S+ + ++ +I L + L SP + + A P P + +P F LI+ +QGR V+ L KP LGFSVVGL+SE++GELGI+VQEIQ +A +DG+L E DQILAI+GQ LD I+H QAI+ILQKA+ V LV+ARGS S P + S S P W +E IEL N+G+G GFG++ G +A GV+VKT++ GVADQ GRL +GD+IL+I D L G EQVA VLR+ GN V+L++AR
Sbjct: 33 LSLLKSVLQSPLFSQILSLQTSVQQLKDQVNIATSA-TSNIEYAHVPHLSPAVIPTLQNESFLLSPNNGNLEALTGPG-------IPHINGKPACDEFDQLIKNMAQGRHVEVFELLKPPSGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQALDQTITHQQAISILQKAKDTVQLVIARGSLPQLVSPIVSRSPSAASTISAHSNP----VHWQHMETIELVNDGSGLGFGIIGG-KATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIAR 335 HSP 4 Score: 130.954 bits (328), Expect = 8.679e-29 Identity = 165/643 (25.66%), Postives = 281/643 (43.70%), Query Frame = 0
Query: 471 VELVKD-AQGLGITIAG------YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIAN--SQSNSQATTPSVSQVAVSATPL---ARSSPQGV------DNEVDNKGESGGDSELSESSESLQDLENQPEI-FSETSEEYWKNQLGSGHAIL---MATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQ-VDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRT 1090
VEL ++ ++ LGI+I G E + GIF+K V + S A K+G + D+I+EVDG ++ S++QAVE +R G V + ++ + L + N + N +T P + ++A + S P+ E G D S +S+ QD++ + E +S WKN + + L + I+ +K + GLG+SL G ++ +I + PNG G +G L+ DELLE+NG+ L G H + +I+K P VK++ R++ Q G + +N+E + +++ TVT S S K+ + +EL K + GLG +I + +D ++ ++I+SL GV+ DGRL GD+++ V+D + +++ + LK A + V++ + P DS S++ + NSS + P SP PE + TS+ A F S +P P +P E TI + K LG+ V D + +++ V G +DGR++ GD ++ VNG +RKA+H +A+++LR+T
Sbjct: 1152 VELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSIINRPRKSPLPSLLHNLYPKYNFSSTNPFADSLQINADKAPSQSESEPEKAPLCSVPPPPPSAFAEMGSDHTQSSASKISQDVDKEDEFGYS------WKN-IRERYGTLTGELHMIELEKGHSGLGLSLAGNKDR-----SRMSVFIVGIDPNGAAGKDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIRNKD-----AVNQMAVCPGNAVEPLPSNSENLQNKETE------PTVTTSDAAVDLSSFKN---------------VQHLELPKDQGGLGIAISE-EDTLSG----VIIKSLTEHGVAATDGRLKVGDQILAVDDEIVVGYPIEKFISLLKTA-KMTVKLTIHAENP-----------DSQAVPSAAGAASGEKKNSSQ---------------------SLMVPQSGSPEPES-----------IRNTSRSSTPAIFAS--DPA----TCPIIPGC-ETTIEISKGRTGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQT 1700 HSP 5 Score: 114.775 bits (286), Expect = 6.270e-24 Identity = 110/415 (26.51%), Postives = 188/415 (45.30%), Query Frame = 0
Query: 155 EGHS-LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSP--------PETTALPSRESTPRVLNSD-------WCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR 553
+GHS LG S+ G K + + +++ I P G A +DG+L D++L I+GQ L SH A +I++ A +V ++ R ++ P + L ++E+ P V SD + V+ +EL + G G + +GV++K++ ++GVA DGRL GD IL ++D + G+ IE+ ++L+ + V+L + E P +PS+ A E + Q +V S G P ++ N +++ +I+ + D T PI +P ET +E+ K GLG++I G + L I + V + AA K G + DQI+EV+G ++ ++ +A+ +LR T + VRL L R
Sbjct: 1355 KGHSGLGLSLAGNKD--RSRMSVFIVGIDPNGAAGKDGRLQIADELLEINGQILYGR-SHQNASSIIKCAPSKVKIIFIRNKDAVNQMAVCPGNAVEPLPSNSENLQNKETEPTVTTSDAAVDLSSFKNVQHLELPKDQGGLGIAISEEDTLSGVIIKSLTEHGVAATDGRLKVGDQILAVDDEIVVGYPIEKFISLLKTAKMTVKLTIHAE---------NPDSQAVPSAAGAASGEKKNSSQSLMVPQS--------GSPEPESIRN----TSRSSTPAIF-------ASDPATCPI-------------------------IPGCET-TIEISKGRTGLGLSIVGGS--DTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYR 1710 HSP 6 Score: 114.005 bits (284), Expect = 9.712e-24 Identity = 90/320 (28.12%), Postives = 147/320 (45.94%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR------------------------EDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVP----------GATGGTMKTVELKRGQHGLGFSIVGGFG----SPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
GLG S+VG G IF+ E+ E AH+DGRLK DQIL + + L + ++H A+ L++ + V+L+I R + ++ ++ GLG I+G K+ GV + ++ GG AD GRL GD I+ + DL S E A +L+ ++ + + R + R + SS P++ P G T VEL + GLG +I G G P G I+VK++ + A G ++ GDQI++V+ L+G ++ AV +L++ V+L ++
Sbjct: 147 GLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQALDQTITHQQAISILQKAKDTVQLVIARGSLPQLVSPIVSRSPSAASTISAHSNPVHWQHMETIELVNDGSGLGFGIIGGKA-TGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAIEERTAPT-ALGITLSSSPTSTPELRVDASTQKGEESETFD-VELTKNVQGLGITIAGYIGDKKLEPSG---IFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVLLTLM 460 HSP 7 Score: 107.842 bits (268), Expect = 7.659e-22 Identity = 150/606 (24.75%), Postives = 247/606 (40.76%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSES--------TQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQ------QAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNK-----GESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPM-TVKLVCAR 740
+G LGF ++G K+ G+ V+ I P G+A Q G+L GD IL I L + +S Q +L++ RV L++ARG+ E+ + + SP T L ST + S+ VE LT N G G +AG + +G+ VK++ ++ + DGR+ GD I+ ++ LQGF +Q VLR +G V L + R +A L R ++ ++ V S L++ N ILPT+ E + L +++ ++E + T + +MG++ E+ V + GLGI++ F++SV SG + D+++EV+G ++ G ++Q V +L+ V + R T P +Q + + L D E+ K GE G SE + ++ D E E + G H I+ +K + GLG S+ + +D + IRS++P G +G L GD L+ VN L + V LK P TV++ A+
Sbjct: 263 DGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDL-AGMSSEQVAQVLRQCGNRVKLMIARGAIEERTAPTALGITLSSSPTSTPELRVDASTQKGEESETFDVE---LTKNVQGLGI-TIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVLLTLMRRGMKQEAE-------------LMSREDVTKDADLSPVNASIIKENYEKDEDFLSSTRNTNILPTEEEGYPL-----LSAEIEEIEDAQKQEAALLTKWQ-----RIMGINY----EIVVAHVSKFSENSGLGISLEATVGHH------FIRSVLPEGPVGHSGKLFSGDELLEVNGITLLGENHQDVVNILKELPIEVTMVCCRR----------------------TVPPTTQSELDSLDLC-------DIELTEKPHVDLGEFIGSSETEDPVLAMTDAGQSTE---EVQAPLAMWEAGIQH------IELEKGSKGLGFSILDYQDPID--PASTVIIIRSLVPGGIAEKDGRLLPGDRLMFVNDVNLENSSLEEAVEALKGAPSGTVRIGVAK 785 HSP 8 Score: 107.457 bits (267), Expect = 1.176e-21 Identity = 183/811 (22.56%), Postives = 330/811 (40.69%), Query Frame = 0
Query: 136 LIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQK---------------------------ARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-----------GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHI-PILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARS--KMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSE---EYWKNQLGSGHAILMATIQKQKPNGGLGISL--EGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPL------TSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPR 894
++Q S+ + I ++K SLG +V S +K LG+ V+ I G ++DG++ GD IL+I+ + S +++ QA +L++ GRV + S + + P S+W Q +EL G G G + G+ G+ +K V+++ A ++G L GD I++++ L+ EQ +RK+GN V +V ++ S + + + P + LQI N+ + S P L + + P + H S + + + +E G + ++ + + +L G E +EL K GLG+++AG +R +S +F+ + AA K G + + D+++E++G+ + G S+Q A +++ V++ +R N A V+Q+AV P P L +SE+LQ+ E +P + + + +KN + ++ K GGLGI++ E T+ V I+S+ +G +G LK GD++L V+ ++G +++LK MTVKL S +V + G E +++ ++ +S S +P+ + SRS P + + IE+ KG GLG SI+ D + I+I + G + +DGRL GD+++ VN I+L A+ DEA+ L+ P+
Sbjct: 996 MLQNVSKESFERTINIAKGNS-SLGMTV----SANKDGLGMIVRSIIHGGAISRDGRIAIGDCILSINEESTIS-VTNAQARAMLRRHSLIGPDIKITYVPAEHLEEFKISLGQQSGRVMALDIFSSYTGRDIPELPEREEGEGEESELQNTAYSNWNQPRRVELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSIINRPRKSPLPSLLHNLYPKYNFSSTNPFADSLQI----NADKAPSQSESEPEKAPLCS--VPPPPPSAFAEMGSDHTQSSASKISQDVDKEDEFGYSWKNIRERYGTLTG---------ELHMIELEKGHSGLGLSLAGNK-DRSRMS-VFIVGIDPNGAAGKDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIR-------------------NKDA----VNQMAV--CPGNAVEP------------------LPSNSENLQNKETEPTVTTSDAAVDLSSFKN---------VQHLELPKDQGGLGIAISEEDTLSGV---------IIKSLTEHGVAATDGRLKVGDQILAVDDEIVVGYPIEKFISLLKTAKMTVKLTIHAENPDSQAVPSA-----AGAASGEKKNSSQSLMVPQS------------GSPEPESIRNTSRSSTPAIFASDPATCPIIPGCETTIEISKGRTGLGLSIVGGSDTL---LGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQ 1702 HSP 9 Score: 92.4337 bits (228), Expect = 3.861e-17 Identity = 67/204 (32.84%), Postives = 98/204 (48.04%), Query Frame = 0
Query: 2007 VELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNS-SQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT----MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
EL K GLG S+VG +S G+F+ E+ +G A DGRL + DQI+ +NG L + + + A IL+ + + + + R G+ Q V + SR S S + + M+T+EL GLGF I+GG + + VKT+ G A HG L GD IL + L G+S +L+ C V L I
Sbjct: 138 FELLKPPSGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQALDQTITHQQAISILQKAKDTVQLVIAR---GSLPQLVSPIVSRSPSAASTISAHSNPVHWQHMETIELVNDGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMI 333 HSP 10 Score: 90.1225 bits (222), Expect = 2.147e-16 Identity = 64/200 (32.00%), Postives = 91/200 (45.50%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHR--------------------------------EDDEIYETLDVELNKKKDRGLGLSIVG----KKSGP-GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
GLG I+GG T + + + G A + GRL GD IL++ DL +S LRQ NRV+LMI R + E ET DVEL K +GLG++I G KK P G+F+ + K A + DGR+ GDQII V+G +L + + A +L+ T + + + R
Sbjct: 266 GLGFGIIGGKAT---GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAIEERTAPTALGITLSSSPTSTPELRVDASTQKGEESETFDVELTKNV-QGLGITIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVLLTLMR 461 HSP 11 Score: 89.7373 bits (221), Expect = 2.584e-16 Identity = 78/257 (30.35%), Postives = 122/257 (47.47%), Query Frame = 0
Query: 141 SQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAID-----GQPLDSNISHHQAINILQKARGRVHLVV------------ARGSA--EDSESTQS---------PPETTALPSRESTPRVLNSD--------WCQVEVIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQV 359
S ++V+H+ L K +G LG ++ SE G+ ++ + G+AA DG+L GDQILA+D G P++ IS +L+ A+ V L + A G+A E S+QS PE+ SR STP + SD C+ IE++ G G ++ G +++ V + G A +DGRL AGD IL++N L+ ++ NVLR++ VRL + R+ P +V
Sbjct: 1476 SSFKNVQHLELPKDQG-GLGIAI----SEEDTLSGVIIKSLTEHGVAATDGRLKVGDQILAVDDEIVVGYPIEKFIS------LLKTAKMTVKLTIHAENPDSQAVPSAAGAASGEKKNSSQSLMVPQSGSPEPESIRNTSRSSTPAIFASDPATCPIIPGCET-TIEISKGRTGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYRDEAPYKEEEV 1720 HSP 12 Score: 89.3521 bits (220), Expect = 3.679e-16 Identity = 70/210 (33.33%), Postives = 98/210 (46.67%), Query Frame = 0
Query: 159 LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR--------EVDPSDASQV 359
LG S+VG G I + E+ +G A +DG+L GDQIL ++G L +H +AIN+L++ RV L + R A P +E + C IEL G G G ++ + GV V +V+ G+AD DGRL GD IL +N ++ E VA +L+ S V L V R E PS +SQV
Sbjct: 1639 LGLSIVGGSDTLLG--AIIIHEVYEEGAACKDGRLWAGDQILEVNGIDL-RKATHDEAINVLRQTPQRVRLTLYRDEA---------------PYKEE-------EVCDTLTIELQKKPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRNATQEAVAALLKCSLGTVTLEVGRIKAGPFHSERRPSQSSQV 1823 HSP 13 Score: 88.5817 bits (218), Expect = 5.745e-16 Identity = 65/216 (30.09%), Postives = 108/216 (50.00%), Query Frame = 0
Query: 140 ASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHL-VVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWC---QVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
AS+ + ++ + + K SLG S+ G G++ I++ + P G+AAQ +L GD+I+ I G + ++H QA+N+L+ A G + + VVA G Q P +++L T + D Q + I L G GF ++ G + + VKTV G A +DGRL GD I+ +N L+G E+ +L+++ V L+V
Sbjct: 1854 ASEIQGLRTVEMKKGPTDSLGISIAGGVGSPLGDVPIFIAMMHPTGVAAQTQKLRVGDRIVTICGTSTE-GMTHTQAVNLLKNASGSIEMQVVAGGDVSVVTGHQQEPASSSLSFTGLTSSSIFQDDLGPPQCKSITLERGPDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAASEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2068 HSP 14 Score: 70.4774 bits (171), Expect = 1.818e-10 Identity = 38/100 (38.00%), Postives = 62/100 (62.00%), Query Frame = 0
Query: 1016 ALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRAS 1115
+ ERTI + K + +LG+ V +G+ G++VRS+ GG +SRDGRI GD ++++N E +++QA +LRR +I I+ITY+PA ++ S
Sbjct: 1004 SFERTINIAKGNSSLGMTVSANKDGL-GMIVRSIIHGGAISRDGRIAIGDCILSINEESTISVTNAQARAMLRRHSLI--GPDIKITYVPAEHLEEFKIS 1100 HSP 15 Score: 69.707 bits (169), Expect = 3.061e-10 Identity = 68/273 (24.91%), Postives = 127/273 (46.52%), Query Frame = 0
Query: 1567 DEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHK--DRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDR-HHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHK----DNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS-DSPSAI-----TAPVSPPAPVVVPTTPQTESAD 1825
DEF +++K A+ + ++ L + GLG S+ G + +R ++ +++ + A RD R++ D IL +NG + H S ++ D+ V ++ R S+ L +S+ P NP+ ++ + + ++ GLG II GK +GV V + PG AD G L GD IL + D G++ E A++L+ +K+++A G ++ +P+A+ ++P S P V +T + E ++
Sbjct: 120 DEF----DQLIKNMAQGRHVEVFELLKPPSGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQALDQTITHQQAISILQKAKDT-VQLVIAR--GSLPQLVSPIVSRSPSAASTISAHSNPVH-----WQHMETIELVNDGSGLGFGIIGGK----ATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIAR--GAIEERTAPTALGITLSSSPTSTPELRVDASTQKGEESE 374 HSP 16 Score: 64.3142 bits (155), Expect = 1.357e-8 Identity = 61/221 (27.60%), Postives = 98/221 (44.34%), Query Frame = 0
Query: 1590 VSLNKG-TNGLGISLAGHKDR--SKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSIL---------------KGEQGLGIMIIEGK---HADVGSGVFVSD-LQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIV 1788
V + KG T+ LGIS+AG + ++I +HP G A + +++VGD I+ + G H + +KN S ++ D S+ H+ P S + GL SI +G GLG I+ G H D+ ++V G+ ++ L+RGD I+AVNG+ GV +E A ILK ++G + ++V
Sbjct: 1863 VEMKKGPTDSLGISIAGGVGSPLGDVPIFIAMMHPTGVAAQTQKLRVGDRIVTICGTSTEGMTHTQAVNLLKNASGSIEMQVVAGGDVSVVT------------GHQQEPASS-SLSFTGLTSSSIFQDDLGPPQCKSITLERGPDGLGFSIVGGYGSPHGDL--PIYVKTVFAKGAASEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2068 HSP 17 Score: 63.929 bits (154), Expect = 1.821e-8 Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 0
Query: 1922 PMGLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI 1994
P GLG SIVGG + G I++ V+ KGAA +DGRLK GDQI+ V + L V+H A+ L++T V LM+
Sbjct: 1994 PDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAASEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2068
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|68052763|sp|Q8VBX6.2|MPDZ_MOUSE (RecName: Full=Multiple PDZ domain protein; AltName: Full=Multi-PDZ domain protein 1) HSP 1 Score: 456.062 bits (1172), Expect = 3.701e-129 Identity = 319/911 (35.02%), Postives = 463/911 (50.82%), Query Frame = 0
Query: 1342 NWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDPVPTPVMTG---SEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKP--------LSQFPPEPHKDNPIERYSGKYKGL------HEVSILKGEQGLGIMIIEGKHADVGSGVFVSDL-QPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLP--PKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVG----NRK-------------------QGVGGMHSRDSSIPSNVPGATGGTMKTVELKRG-QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
+W R V + R P++ LGISIVGG+ S N V+ GIFIK+VLE SPAG + L GDRI+ VDG DL A+H+QAV+ IR++G V F+VQS+ + + S HS + +S P L P + P + S S G D S+++ S DED+ EDEFGYS + I ++Y G + L KG +GLG+SLAG+KDR++MSV+I G+ P G A RD R+Q+ D +LE+NG ++Y R H N SS IK P S V I +R+ +++ +AV P + P+ + P S L +++ + K + GLGI I E D +GV + L + G A L+ GD ILAV+ E G E +LK ++ +K+ V ++++P+ P P + V V E D + P P P + P P + S + P + +S+PATC I PG +T IEISK + GLGLSIVGGSDTLLGAI IHEVYE+GAA KDGRL GDQILEV DLR +H A++ LRQTP RVRL ++R+ ++++ +T +EL K+ +GLGLSIVGK++ GVF+S++VKGG AD+DGRL+QGDQI+ VNG D+ +++QE A +LK + G + + V R++ R+ G+ S +S+ N + ++TVE+K+G LG SI GG GSP GD+PI++ + G AA L+ GD+I+++ +G++H AVN++KN G++ +Q+++
Sbjct: 1133 SWSQPRRVELWREPSKSLGISIVGGR-------GMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIIEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSI--INRPRKSPLPSLPHSLYPKYSFSSTNPFADSLQLTTDQAPSQSESETEKPALCNVPPSSPSVFSEMGSDCAQPSATAVSEDEDK------------------------------------EDEFGYSWKNIQERYGSLTGQLHVIELEKGQSGLGLSLAGNKDRTRMSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAP-SKVKIIFIRNADAVNQMAVCPGIAADSPSSTSDSPQNKEVEPCSTTSASAADLSSLTDVYQLELPKDQGGLGIAICE---EDTINGVMIESLTEHGGAAKDGRLKPGDHILAVDDEVVAGCPVEKFISLLKTAKATVKLTV-------RAENPAC---PAVPSSAVTV----SGERKDNSQTPAVPAPDLEPIP-STSRSSTPAVF------------------------------------------ASDPATCPIIPGCETTIEISKGQ------TGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYRDEAPYKEEDVCDTFTIELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRHATQEAVAALLKCSLGAVTLEVGRVKAAPFHSERRPSQSSQVSESSLSSFTPPLSGINTSESLESNSKKNALASEIQGLRTVEIKKGPADSLGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNASGSIEVQVVA 1931 HSP 2 Score: 326.25 bits (835), Expect = 1.649e-88 Identity = 201/444 (45.27%), Postives = 263/444 (59.23%), Query Frame = 0
Query: 465 EMETLDVELVKDAQGLGITIAGYTCERE-ELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPL 907
E ET DVEL K+ QGLGITIAGY +++ E SGIFVKS+T SA G + + DQII VDG ++QGF+NQQAVE+LR TG++VRL L+R G E + ++ + + + S + +S ++ E G LS E +D++ + + ++ W+ +G + I++A + K N GLGISLE TV HH+IRSVLP GPVG +G L SGDELLEVNG LLG H DVV ILK+LP+ V +VC R ++ + + E K D+ + TE S + + E T LAMW IEL KG RGLGFSILDYQDP++ TVIVIRSLVPGG++++DGRL PGDRLM+VNDINLE+++L+EAV+ALKGAP GMVRIGV KPLPL
Sbjct: 373 ESETFDVELTKNVQGLGITIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMR--KGASQEAELTSRGDTAKDVDLPAENCEKDEESLSLKRNTSILPIEEE--------GFPLLSAELEEAEDVQQEAALLTK-----WQRIMGINYEIVVAHVSKFSENSGLGISLEATV---------GHHFIRSVLPEGPVGHSGKLFSGDELLEVNGINLLGENHQDVVNILKELPIDVTMVCCRRTVPPIALSEMDSLDIN---------DLELTEKPHIDLGEFIGSSETEDPMLAM-SDVDQNAEEIQTPLAMWEAGGQSIELEKGSRGLGFSILDYQDPIDPANTVIVIRSLVPGGIAEKDGRLFPGDRLMFVNDINLENSTLEEAVEALKGAPSGMVRIGVAKPLPL 782 HSP 3 Score: 228.794 bits (582), Expect = 1.236e-58 Identity = 143/312 (45.83%), Postives = 187/312 (59.94%), Query Frame = 0
Query: 43 LATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIG---VNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALP--SRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
L+ L SVL+SP+F ILN+Q SL +LK QV + + + +I NL L SP H + A P +P P + LI+ +QGR V+ L KP LGFSVVGL+SE++GELGI+VQEIQ +A +DG+L E DQILAI+GQ LD I+H QAI+ILQKA+ V LV+ARGS S + +A S S P W VE IEL N+G+G GFG++ G +A GV+VKT++ GVADQ GRL +GD+IL+I D L G EQVA VLR+ GN V+L++AR
Sbjct: 33 LSLLKSVLQSPLFSQILNLQTSLQQLKDQVNIATLATAAADHAHTPQFSSAVISNLQSESLLLSPNHGNLEALPGPGAPAVMDGKPTCD---ELDQLIKNMAQGRHVEIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIARGSLPPVSSPRISRSPSAASTISAHSNPM----HWQHVETIELVNDGSGLGFGIIGG-KATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIAR 336 HSP 4 Score: 126.331 bits (316), Expect = 2.140e-27 Identity = 171/657 (26.03%), Postives = 279/657 (42.47%), Query Frame = 0
Query: 471 VELVKD-AQGLGITIAG------YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYV------------HGL--KFE-----------QLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKN---QLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLP-LPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQ-VDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRT 1090
VEL ++ ++ LGI+I G E + GIF+K V + S A K+G + D+IIEVDG ++ S++QAVE +R G V + + H L K+ QL A SQS S+ P++ V S+ + E G D S+ ++ + E++ + F + WKN + GS L I+ +K GLG+SL G ++ +I + P G G +G L+ DELLE+NG+ L G H + +I+K P VK++ R+ A + V S+ + + + + + S S L+SL + ++EL K + GLG +I + +D +N ++I SL G + +DGRL PGD ++ V+D + +++ + LK A + V++ V P P V S+ S DN++ +P+V PDL PIP +TS+ A F S + P +P E TI + K LG+ V D + +++ V G +DGR++ GD ++ VNG +RKA+H +A+++LR+T
Sbjct: 1140 VELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIIEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSIINRPRKSPLPSLPHSLYPKYSFSSTNPFADSLQLTTDQAPSQSESETEKPALCNVPPSSPSVF--------------SEMGSDCA-QPSATAVSEDEDKEDEFGYS----WKNIQERYGSLTGQLH-VIELEKGQSGLGLSLAGNKDRT-----RMSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIRNAD----------------------AVNQMAVCPGIAADSPSSTSDSPQNKEVEPCSTTSASAADLSSLT----DVYQLELPKDQGGLGIAICE-EDTING----VMIESLTEHGGAAKDGRLKPGDHILAVDDEVVAGCPVEKFISLLKTA-KATVKLTVRAENPACPAVP-------SSAVTVSGERKDNSQ-----------TPAVPAPDL-----EPIP------------------------STSRSSTPAVFAS------DPATCPIIPGC-ETTIEISKGQTGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQT 1685 HSP 5 Score: 109.383 bits (272), Expect = 2.714e-22 Identity = 91/318 (28.62%), Postives = 146/318 (45.91%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR------------EDDEIYETLDVELNKKKDR------------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRV----GNRKQGVGGMHSRDSSIPSNVPGATGGTMKT----VELKRGQHGLGFSIVGGFG----SPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
GLG S+VG G IF+ E+ E AH+DGRLK DQIL + + L + ++H A+ L++ + V+L+I R T+ N + GLG I+G K+ GV + ++ GG AD GRL GD I+ + DL S E A +L+ ++ + + R V + G+ S S+ V +T ++ VEL + GLG +I G G P G I+VK++ + A G ++ GDQI++V+ L+G ++ AV +L++ V L ++
Sbjct: 148 GLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIARGSLPPVSSPRISRSPSAASTISAHSNPMHWQHVETIELVNDGSGLGFGIIGGKA-TGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPASSSLGITLSSSTSSTSEMRVDASTQKNDESETFDVELTKNVQGLGITIAGYIGDKKLEPSG---IFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLM 461 HSP 6 Score: 107.457 bits (267), Expect = 1.116e-21 Identity = 147/597 (24.62%), Postives = 250/597 (41.88%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNS-----DWCQVEVIELTNNGAGFGFGVMAGQ------QAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVD-NEVD-------NKGESGGDSELSESSESLQDL-ENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLP 731
+G LGF ++G K+ G+ V+ I P G+A Q G+L GD IL I L + +S Q +L++ RV L++ARG+ E++ ++ S T + + ++ +++ D + +ELT N G G +AG + +G+ VK++ ++ + DGR+ GD I+ ++ LQGF +Q VLR +G VRL + R+ ASQ + L R + + + L+ N ILP + E + L +++ QE L+ R +MG++ E+ V + GLGI++ F++SV SG + D+++EV+G ++ G ++Q V +L+ V + R T P P+A S +D N+++ + GE G SE + ++ D+ +N EI +T W+ G +I+ +K + GLG S+ + +D N IRS++P G +G L GD L+ VN L + V LK P
Sbjct: 264 DGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDL-AGMSSEQVAQVLRQCGNRVKLMIARGAVEETPASSSLGITLSSSTSSTSEMRVDASTQKNDESETFDVELTKNVQGLGI-TIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMRK----GASQ---------EAELTSRGDTAKDVDLP-------AENCEKDEESLSLKRNTSILPIEEEGFPL-----LSAELEEAEDVQQEAALLTKWQR------IMGINY----EIVVAHVSKFSENSGLGISLEATVGHH------FIRSVLPEGPVGHSGKLFSGDELLEVNGINLLGENHQDVVNILKELPIDVTMVCCRR----------------------TVP----------PIALSEMDSLDINDLELTEKPHIDLGEFIGSSETEDPMLAMSDVDQNAEEI--QTPLAMWE---AGGQ-----SIELEKGSRGLGFSILDYQDPID--PANTVIVIRSLVPGGIAEKDGRLFPGDRLMFVNDINLENSTLEEAVEALKGAP 768 HSP 7 Score: 98.5969 bits (244), Expect = 4.538e-19 Identity = 102/410 (24.88%), Postives = 179/410 (43.66%), Query Frame = 0
Query: 159 LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSP--------PETTALPSRESTPRVLNS-------DWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR 553
LG S+ G K + + +++ I P G A +DG+L D++L I+GQ L SH A +I++ A +V ++ R + ++ P + + ++E P S V +EL + G G + GV+++++ ++G A +DGRL GD+IL ++D + G +E+ ++L+ + V+L V E P P +PSS + E + + Q + V A P P T +++ +++ + D T PI +P ET +E+ K GLG++I G + L I + V + AA K G + DQI+EV+G ++ ++ +A+ +LR T + VRL L R
Sbjct: 1348 LGLSLAGNKDRTR--MSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGR-SHQNASSIIKCAPSKVKIIFIRNADAVNQMAVCPGIAADSPSSTSDSPQNKEVEPCSTTSASAADLSSLTDVYQLELPKDQGGLGIAICEEDTINGVMIESLTEHGGAAKDGRLKPGDHILAVDDEVVAGCPVEKFISLLKTAKATVKLTVRAE---------NPACPAVPSSAVTVSGERKDNSQ------TPAVPAPDLEPIPST---------SRSSTPAVF-------ASDPATCPI-------------------------IPGCET-TIEISKGQTGLGLSIVGGS--DTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYR 1695 HSP 8 Score: 95.5153 bits (236), Expect = 4.010e-18 Identity = 150/669 (22.42%), Postives = 262/669 (39.16%), Query Frame = 0
Query: 263 SDWCQVEVIELTNN-----------GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVD-------PSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISL--EGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVT-ESSHQPQQSKLKSRSLEPLTSLAMWSDTLL----------------EIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPR 894
S W Q +EL G G G + G+ G+ +K V+++ A ++G L GD I++++ L+ EQ +RK+GN V +V ++ PS + P ++ A +L T + + + A + PP ++ ++M QPS S D+ ++E + + SL G + +EL K GLG+++AG +R +S +F+ + AA + G + + D+++E++G+ + G S+Q A +++ V++ +R + N A P ++A + +S + EV+ S +S S DL + +++ ++ K GGLGI++ E T+ V I S+ +G +G LK GD +L V+ + G +++LK TVKL T R SSA TV+ E Q + + LEP+ S + S + IE+ KG+ GLG SI+ D + I+I + G + +DGRL GD+++ VN I+L A+ DEA+ L+ P+
Sbjct: 1132 SSWSQPRRVELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIIEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSIINRPRKSPLPSLPHSLYPKYSFSSTNPFADSLQLTTDQAPSQSESETEKPALCNVPPSSPSVFSEM-------GSDCAQPSATAVSEDEDK---EDEFGYSWKNIQERYGSLTG---------QLHVIELEKGQSGLGLSLAGNK-DRTRMS-VFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIRNADAV--------------NQMAVCP-----GIAADSPSSTSDSPQNKEVE---------PCSTTSASAADLSSLTDVYQ---------------------LELPKDQGGLGIAICEEDTINGV---------MIESLTEHGGAAKDGRLKPGDHILAVDDEVVAGCPVEKFISLLKTAKATVKL-------------------------------TVRAENPACPAVPSSAVTVSGERKDNSQTPAVPAPDLEPIPSTSRSSTPAVFASDPATCPIIPGCETTIEISKGQTGLGLSIVGGSDTLLG---AIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQ 1687 HSP 9 Score: 90.1225 bits (222), Expect = 1.954e-16 Identity = 65/201 (32.34%), Postives = 93/201 (46.27%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHR---------------------------------EDDEIYETLDVELNKKKDRGLGLSIVG----KKSGP-GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
GLG I+GG T + + + G A + GRL GD IL++ DL +S LRQ NRV+LMI R ++DE ET DVEL K +GLG++I G KK P G+F+ + K A + DGR+ GDQII V+G +L + + A +L+ T + + + R
Sbjct: 267 GLGFGIIGGKAT---GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPASSSLGITLSSSTSSTSEMRVDASTQKNDE-SETFDVELTKNV-QGLGITIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMR 462 HSP 10 Score: 88.1965 bits (217), Expect = 6.562e-16 Identity = 65/192 (33.85%), Postives = 91/192 (47.40%), Query Frame = 0
Query: 159 LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
LG S+VG G I + E+ +G A +DG+L GDQIL ++G L +H +AIN+L++ RV L + R A P +E D C IEL G G G ++ + GV V +V+ G+AD DGRL GD IL +N ++ E VA +L+ S V L V R
Sbjct: 1624 LGLSIVGGSDTLLG--AIIIHEVYEEGAACKDGRLWAGDQILEVNGIDL-RKATHDEAINVLRQTPQRVRLTLYRDEA---------------PYKEE-------DVCDTFTIELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRHATQEAVAALLKCSLGAVTLEVGR 1790 HSP 11 Score: 85.8853 bits (211), Expect = 4.140e-15 Identity = 68/231 (29.44%), Postives = 110/231 (47.62%), Query Frame = 0
Query: 127 LENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARG------SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
LE+ + AL AS+ + ++ + + K SLG S+ G G++ I++ + P G+AAQ +L GD+I+ I G D ++H QA+N+++ A G + + V G + E T L S P L Q + I L G GF ++ G + + VKTV G A +DGRL GD I+ +N L+G E+ +L+++ V L+V
Sbjct: 1829 LESNSKKNAL---ASEIQGLRTVEIKKGPADSLGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTD-GMTHTQAVNLMKNASGSIEVQVVAGGDVSVVTGHQQELANPCLAFTGLTSSSIFPDDLGPP--QSKTITLDRGPDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2053 HSP 12 Score: 80.1073 bits (196), Expect = 2.175e-13 Identity = 64/208 (30.77%), Postives = 98/208 (47.12%), Query Frame = 0
Query: 2000 EIYETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNS-SQEDAAPILKMTQGKIAMRVRRLRV-GNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
EI+E L K GLG S+VG +S G+F+ E+ +G A DGRL + DQI+ +NG L + + + A IL+ + + + + R + + S S+I ++ ++T+EL GLGF I+GG + + VKT+ G A HG L GD IL + L G+S +L+ C V L I
Sbjct: 137 EIFELL-----KPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIARGSLPPVSSPRISRSPSAASTISAHSNPMHWQHVETIELVNDGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMI 334 HSP 13 Score: 74.3294 bits (181), Expect = 1.067e-11 Identity = 40/100 (40.00%), Postives = 62/100 (62.00%), Query Frame = 0
Query: 1016 ALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRAS 1115
A ERT+ + K S +LG+ V +G+ G++VRS+ GG +SRDGRI GD ++++N E +++QA +LRR +I I+ITY+PA +R S
Sbjct: 992 AFERTVTIAKGSSSLGMTVSANKDGL-GVIVRSIIHGGAISRDGRIAVGDCILSINEESTISLTNAQARAMLRRHSLI--GPDIKITYVPAEHLEEFRVS 1088 HSP 14 Score: 64.3142 bits (155), Expect = 1.165e-8 Identity = 57/227 (25.11%), Postives = 101/227 (44.49%), Query Frame = 0
Query: 1575 KILKKYARAKGTPIYVSLNKGTNGLGISLAGHK--DRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDR-HHLNVSSFIKNLPDSDVTFILLRSD--------SSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVA 1789
+++K A+ + I+ L GLG S+ G + +R ++ +++ + A RD R++ D IL +NG V+ H S ++ D+ V ++ R S A +S NP+ ++ + + ++ GLG II GK +GV V + PG AD G L GD IL + D G++ E A++L+ +K+++A
Sbjct: 125 QLIKNMAQGRHVEIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDT-VQLVIARGSLPPVSSPRISRSPSAASTISA------HSNPMH-----WQHVETIELVNDGSGLGFGIIGGK----ATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIA 335 HSP 15 Score: 63.929 bits (154), Expect = 1.898e-8 Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 0
Query: 1922 PMGLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI 1994
P GLG SIVGG + G I++ V+ KGAA +DGRLK GDQI+ V + L V+H A+ L++T V LM+
Sbjct: 1979 PDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2053 HSP 16 Score: 63.5438 bits (153), Expect = 2.064e-8 Identity = 57/217 (26.27%), Postives = 100/217 (46.08%), Query Frame = 0
Query: 1590 VSLNKG-TNGLGISLAGH--KDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGL-----------HEVSILKGEQGLGIMIIEGK---HADVGSGVFVSD-LQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIV 1788
V + KG + LG+S+AG + ++I +HP+G A + +++VGD I+ + G H + +KN S ++ D S+ + L+ NP ++G +++ +G GLG I+ G H D+ ++V G+ A+ L+RGD I+AVNG+ GV +E A ILK ++G + ++V
Sbjct: 1848 VEIKKGPADSLGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNASGSIEVQVVAGGDVSVVTGHQQELA---------NPCLAFTGLTSSSIFPDDLGPPQSKTITLDRGPDGLGFSIVGGYGSPHGDLP--IYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2053
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|68052388|sp|O55164.1|MPDZ_RAT (RecName: Full=Multiple PDZ domain protein; AltName: Full=Multi-PDZ domain protein 1) HSP 1 Score: 454.907 bits (1169), Expect = 1.045e-128 Identity = 320/914 (35.01%), Postives = 470/914 (51.42%), Query Frame = 0
Query: 1342 NWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDPVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEE---EDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPH-KDNPIERYS-------------GKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDL-QPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVG----NRK----------------QGVGGMHS--------RDSSIPSNVPGATGGTMKTVELKRG-QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
+W R V + R P++ LGISIVGG+ S N V+ GIFIK+VLE SPAG + L GDRI+ VDG DL A+H+QAV+ IR++G V F+VQS++ + P P P L S+ P + S+T + Q +S + S E E E + + S + + +S P E+ EDEFGYS + I ++Y G + L KG +GLG+SLAG+KDR++MSV+I G+ P G A RD R+Q+ D +LE+NG ++Y R H N SS IK P S V I +R+ +++ +AV P S P P ++P + ++ + + K + GLGI I E D +GV + L + G A L+ GD ILAV+ E G E +LK ++ +K+ V +++P P+ V + + +S+ P + P P + P P + S + P I +S+PATC I PG +T IEISK + GLGLSIVGGSDTLLGAI IHEVYE+GAA KDGRL GDQILEV DLR +H A++ LRQTP RVRL ++R+ ++++ +T VEL K+ +GLGLSIVGK++ GVF+S++VKGG AD+DGRL+QGDQI+ VNG D+ N++QE A +LK + G + + V R++ R+ G+H+ + +++ S + G ++TVE+K+G LG SI GG GSP GD+PI++ + G AA L+ GD+I+++ +G++H AVN++KN G++ +Q+++
Sbjct: 1132 SWSQPRRVELWREPSKSLGISIVGGR-------GMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGSPVVFMVQSIV------------------------NRPRKSPLPSLPHSLYPKCSF-------------SSTNPFAESLQLTSDKAPSQSESESEKA-TLCSVPSSSPSVFSEMSSDYAQPSATTVAEDEDKEDEFGYSWKNIQERYGTLTGQLHMIELEKGHSGLGLSLAGNKDRTRMSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAP-SKVKIIFIRNADAVNQMAVCPGSAADPLPSTSESPQNKEVEPSITTSASAVDLSSLTNVYHLELPKDQGGLGIAICE---EDTLNGVTIKSLTERGGAAKDGRLKPGDRILAVDDELVAGCPIEKFISLLKTAKTTVKLTVG-------AENPGCQAV----PSAAVTASGERKDSSQTPAV-PAPDLEPIP-STSRSSTPAIF------------------------------------------ASDPATCPIIPGCETTIEISKGQ------TGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYRDEAPYKEEDVCDTFTVELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRNATQEAVAALLKCSLGTVTLEVGRIKAAPFHSERRPSQSSQVSESSLSSFSLPRSGIHTSESSESSAKKNALASEIQG-----LRTVEIKKGPADALGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNASGSIEVQVVA 1930 HSP 2 Score: 329.331 bits (843), Expect = 2.053e-89 Identity = 204/450 (45.33%), Postives = 266/450 (59.11%), Query Frame = 0
Query: 465 EMETLDVELVKDAQGLGITIAGYTCERE-ELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRY-----VHGLKFEQLQQAIANSQSNSQATTPSVS-QVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPL 907
E ET DVEL K+ QGLGITIAGY +++ E SGIFVKS+T SA G + + DQI+ VDG ++QGF+NQQAVE+LR TG++VRL L+R E + + N + S+S + + S P+ E G LS E +D++ + + ++ W+ +G + I++A + K N GLGISLE TV HH+IRSVLP GPVG +G L SGDELLEVNG LLG H DVV ILK+LP+ V +VC R T++ + H++ E K D+ + TE S + + E T LAMW + IEL KG RGLGFSILDYQDP++ TVIVIRSLVPGG++++DGRL PGDRLM+VNDINLE+++L+EAV+ALKGAP GMVRIGV KPLPL
Sbjct: 372 ESETFDVELTKNVQGLGITIAGYIGDKKLEPSGIFVKSITKSSAVELDGRIQIGDQIVAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMRKGASQEAEITSREDTAKDVDLPAENYEKDEESLSLKRSTSILPI----------------EEEGYPLLSTELEETEDVQQEAALLTK-----WQRIMGINYEIVVAHVSKFSENSGLGISLEATV---------GHHFIRSVLPEGPVGHSGKLFSGDELLEVNGINLLGENHQDVVNILKELPIDVTMVCCRRTVPPTALSEVDSLDI-----HDL----ELTEKPHIDLGEFIGSSETEDPMLA-MSDVDQNAEEIQTPLAMWEAGIQAIELEKGSRGLGFSILDYQDPIDPANTVIVIRSLVPGGIAEKDGRLFPGDRLMFVNDINLENSTLEEAVEALKGAPSGMVRIGVAKPLPL 781 HSP 3 Score: 229.95 bits (585), Expect = 6.230e-59 Identity = 142/316 (44.94%), Postives = 195/316 (61.71%), Query Frame = 0
Query: 43 LATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIG----VNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEP---RFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALP--SRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
L+ L SVL+SP+F IL++Q SL +LK QV + ++ ++ D + +I NL L SP + + A P +P P ++ +P LI+ +QGR V+ L KP LGFSVVGL+SE++GELGI+VQEIQ +A +DG+L E DQILAI+GQ LD I+H QAI+ILQKA+ + LV+ARGS S + +A S S P + W VE IEL N+G+G GFG++ G +A GV+VKT++ GVADQ GRL +GD+IL+I D L G EQVA VLR+ GN V+L++AR
Sbjct: 33 LSLLKSVLQSPLFSQILSLQTSLQQLKDQVNV-ATLATANADHAHTPQFSSAIISNLQSESLLLSPSNGNLEAISGPGAP------PAMDGKPACEELDQLIKSMAQGRHVEIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTIQLVIARGSLPHISSPRISRSPSAASTVSAHSNP----THWQHVETIELVNDGSGLGFGIIGG-KATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIAR 336 HSP 4 Score: 128.257 bits (321), Expect = 4.622e-28 Identity = 168/659 (25.49%), Postives = 280/659 (42.49%), Query Frame = 0
Query: 471 VELVKD-AQGLGITIAG------YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEV-DNKGESGGDSELSESSESLQDLENQPEIFSETSEEY---------------------WKNQLGSGHAIL---MATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDT-LLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGM-VRIGVTKP----LPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQ-VDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRT 1090
VEL ++ ++ LGI+I G E + GIF+K V + S A K+G + D+I+EVDG ++ S++QAVE +R G V + Q+I N S PS+ + ++P ++ +K S +SE SE + + P +FSE S +Y WKN + + L + I+ +K + GLG+SL G ++ +I + P G G +G L+ DELLE+NG+ L G H + +I+K P VK++ R+ V + S+A + +S PQ +++ +++ + S T + +EL K + GLG +I + +D +N + I+SL G + +DGRL PGDR++ V+D + +++ + LK A + + +G P +P V+ + DS+ T P+V PDL PIP +TS+ A F S +P P +P E TI + K LG+ V D + +++ V G +DGR++ GD ++ VNG +RKA+H +A+++LR+T
Sbjct: 1139 VELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGSPVVF-------------MVQSIVNRPRKS--PLPSLPHSLYPKCSFSSTNPFAESLQLTSDKAPSQSESE-SEKATLCSVPSSSPSVFSEMSSDYAQPSATTVAEDEDKEDEFGYSWKN-IQERYGTLTGQLHMIELEKGHSGLGLSLAGNKDRT-----RMSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIRNAD---------------------------AVNQMAVCPGSAADPLPSTSESPQNKEVEPSITTSASAVDLSSLTNVYHLELPKDQGGLGIAICE-EDTLNG----VTIKSLTERGGAAKDGRLKPGDRILAVDDELVAGCPIEKFISLLKTAKTTVKLTVGAENPGCQAVPSAAVTASGERKDSSQT-----------------------PAVPAPDL-----EPIP------------------------STSRSSTPAIFAS--DPA----TCPIIPGC-ETTIEISKGQTGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQT 1684 HSP 5 Score: 107.457 bits (267), Expect = 1.194e-21 Identity = 111/415 (26.75%), Postives = 183/415 (44.10%), Query Frame = 0
Query: 155 EGHS-LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR------------GSAEDS--ESTQSPPETTALPSRESTPRVLN-SDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR 553
+GHS LG S+ G K + + +++ I P G A +DG+L D++L I+GQ L SH A +I++ A +V ++ R GSA D +++SP PS ++ ++ S V +EL + G G + GV +K++ + G A +DGRL GD IL ++D + G IE+ ++L+ + V+L V E P +PS+ + E + Q + V A P P T +++ +I+ + D T PI +P ET +E+ K GLG++I G + L I + V + AA K G + DQI+EV+G ++ ++ +A+ +LR T + VRL L R
Sbjct: 1342 KGHSGLGLSLAGNKDRTR--MSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGR-SHQNASSIIKCAPSKVKIIFIRNADAVNQMAVCPGSAADPLPSTSESPQNKEVEPSITTSASAVDLSSLTNVYHLELPKDQGGLGIAICEEDTLNGVTIKSLTERGGAAKDGRLKPGDRILAVDDELVAGCPIEKFISLLKTAKTTVKLTVGAE---------NPGCQAVPSAAVTASGERKDSSQ------TPAVPAPDLEPIPST---------SRSSTPAIF-------ASDPATCPI-------------------------IPGCET-TIEISKGQTGLGLSIVGGS--DTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYR 1694 HSP 6 Score: 107.071 bits (266), Expect = 1.523e-21 Identity = 89/322 (27.64%), Postives = 140/322 (43.48%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMI-------------------------HREDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR-----------LRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFG----SPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
GLG S+VG G IF+ E+ E AH+DGRLK DQIL + + L + ++H A+ L++ + ++L+I H +EL GLG I+G K+ GV + ++ GG AD GRL GD I+ + DL S E A +L+ ++ + + R L + D+S N T VEL + GLG +I G G P G I+VK++ + A G ++ GDQI++V+ L+G ++ AV +L++ V L ++
Sbjct: 148 GLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTIQLVIARGSLPHISSPRISRSPSAASTVSAHSNPTHWQHVETIEL-VNDGSGLGFGIIGGKA-TGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPAPSSLGITLSSSTSTSEMRVDASTQKNEESET----FDVELTKNVQGLGITIAGYIGDKKLEPSG---IFVKSITKSSAVELDGRIQIGDQIVAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLM 460 HSP 7 Score: 104.76 bits (260), Expect = 6.532e-21 Identity = 159/650 (24.46%), Postives = 261/650 (40.15%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEV----IELTNNGAGFGFGVMAGQ------QAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNK-----GESGGDSELSESSESLQDL-ENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTE 788
+G LGF ++G K+ G+ V+ I P G+A Q G+L GD IL I L + +S Q +L++ RV L++ARG+ E++ + S T + + S RV S E +ELT N G G +AG + +G+ VK++ ++ + DGR+ GD I+ ++ LQGF +Q VLR +G VRL + R+ +A + A+ E + +++ ++S + G P +L T+ E+ Q QE L+ R +MG++ E+ V + GLGI++ F++SV SG + D+++EV+G ++ G ++Q V +L+ V + R T P + V + + D E+ K GE G SE + ++ D+ +N EI +T W+ + + I+ +K + GLG S+ + +D N IRS++P G +G L GD L+ VN L + V LK P S +G + P L+ E V AK D + S T E
Sbjct: 264 DGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDL-AGMSSEQVAQVLRQCGNRVKLMIARGAVEETPAPSSLGITLSSSTSTSEMRVDASTQKNEESETFDVELTKNVQGLGI-TIAGYIGDKKLEPSGIFVKSITKSSAVELDGRIQIGDQIVAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMRKGASQEAEITSREDTAKDVDLPAENYEKDEE-SLSLKRSTSILPIEEEGYP---------LLSTELEETEDVQ---------------QEAALLTKWQR------IMGINY----EIVVAHVSKFSENSGLGISLEATVGHH------FIRSVLPEGPVGHSGKLFSGDELLEVNGINLLGENHQDVVNILKELPIDVTMVCCRR----------------------TVPPTALSEVDSLDIH-------DLELTEKPHIDLGEFIGSSETEDPMLAMSDVDQNAEEI--QTPLAMWEAGIQA--------IELEKGSRGLGFSILDYQDPID--PANTVIVIRSLVPGGIAEKDGRLFPGDRLMFVNDINLENSTLEEAVEALKGAP-----------SGMVRIGVAKPLP---------LSPEEGYVSAKEDTFLCSPHTCKE 804 HSP 8 Score: 97.4413 bits (241), Expect = 1.116e-18 Identity = 155/665 (23.31%), Postives = 273/665 (41.05%), Query Frame = 0
Query: 263 SDWCQVEVIELTNN-----------GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETH---LQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIY-QPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISL--EGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLL----------------EIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPR 894
S W Q +EL G G G + G+ G+ +K V+++ A ++G L GD I++++ L+ EQ +RK+G+ V +V V+ K +P LP S+ K + T+ + + ++ + + S +E S Y QPS + D+ ++E + + +L G Q M E+E K GLG+++AG +R +S +F+ + AA + G + + D+++E++G+ + G S+Q A +++ V++ +R N+ A V+Q+AV P + + P L +SES Q+ E +P I + S + H ++ K GGLGI++ E T+ V I+S+ G +G LK GD +L V+ + G +++LK TVKL +VG + +A +++++G +SS P + + LEP+ S + S + IE+ KG+ GLG SI+ D + I+I + G + +DGRL GD+++ VN I+L A+ DEA+ L+ P+
Sbjct: 1131 SSWSQPRRVELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIVEVDGMDLRDASHEQAVEAIRKAGSPVVFMVQSIVNRPR----KSPLPSLPHSLYPKCSFSSTNPFAESLQLTSDKAPSQSESESEKATLCSVPSSSPSVFSEMSSDYAQPSATTVAEDEDK---EDEFGYSWKNIQERYGTLTG--QLHMIELE-------KGHSGLGLSLAGNK-DRTRMS-VFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIR-------------------NADA----VNQMAV--CPGSAADP------------------LPSTSESPQNKEVEPSITTSASAVDLSSLTNVYH------LELPKDQGGLGIAICEEDTLNGV---------TIKSLTERGGAAKDGRLKPGDRILAVDDELVAGCPIEKFISLLKTAKTTVKL----------TVGAENPG-----------------CQAVPSAAVTASGERKDSSQTP---AVPAPDLEPIPSTSRSSTPAIFASDPATCPIIPGCETTIEISKGQTGLGLSIVGGSDTLLG---AIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQ 1686 HSP 9 Score: 90.5077 bits (223), Expect = 1.305e-16 Identity = 63/199 (31.66%), Postives = 92/199 (46.23%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHR-------------------------------EDDEIYETLDVELNKKKDRGLGLSIVG----KKSGP-GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
GLG I+GG T + + + G A + GRL GD IL++ DL +S LRQ NRV+LMI R + +E ET DVEL K +GLG++I G KK P G+F+ + K A + DGR+ GDQI+ V+G +L + + A +L+ T + + + R
Sbjct: 267 GLGFGIIGGKAT---GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPAPSSLGITLSSSTSTSEMRVDASTQKNEESETFDVELTKNV-QGLGITIAGYIGDKKLEPSGIFVKSITKSSAVELDGRIQIGDQIVAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMR 461 HSP 10 Score: 88.5817 bits (218), Expect = 5.499e-16 Identity = 64/192 (33.33%), Postives = 91/192 (47.40%), Query Frame = 0
Query: 159 LGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
LG S+VG G I + E+ +G A +DG+L GDQIL ++G L +H +AIN+L++ RV L + R A P +E D C +EL G G G ++ + GV V +V+ G+AD DGRL GD IL +N ++ E VA +L+ S V L V R
Sbjct: 1623 LGLSIVGGSDTLLG--AIIIHEVYEEGAACKDGRLWAGDQILEVNGIDL-RKATHDEAINVLRQTPQRVRLTLYRDEA---------------PYKEE-------DVCDTFTVELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRNATQEAVAALLKCSLGTVTLEVGR 1789 HSP 11 Score: 84.3445 bits (207), Expect = 9.763e-15 Identity = 71/229 (31.00%), Postives = 104/229 (45.41%), Query Frame = 0
Query: 1982 ALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNS-SQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT----MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
A + ++ M EI+E L K GLG S+VG +S G+F+ E+ +G A DGRL + DQI+ +NG L + + + A IL+ + I + + R G+ SR S S V + T ++T+EL GLGF I+GG + + VKT+ G A HG L GD IL + L G+S +L+ C V L I
Sbjct: 119 ACEELDQLIKSMAQGRHVEIFELL-----KPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTIQLVIAR---GSLPHISSPRISRSPSAASTVSAHSNPTHWQHVETIELVNDGSGLGFGIIGGKAT-----GVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMI 334 HSP 12 Score: 83.9593 bits (206), Expect = 1.525e-14 Identity = 63/218 (28.90%), Postives = 104/218 (47.71%), Query Frame = 0
Query: 140 ASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARG------SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
AS+ + ++ + + K +LG S+ G G++ I++ + P G+AAQ +L GD+I+ I G D ++H QA+N+++ A G + + V G + E T L S P L Q + I L G GF ++ G + + VKTV G A +DGRL GD I+ +N L+G E+ +L+++ V L+V
Sbjct: 1838 ASEIQGLRTVEIKKGPADALGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTD-GMTHTQAVNLMKNASGSIEVQVVAGGDVSVVTGHQQELANPCLAFTGLTSSTIFPDDLGPP--QSKTITLDRGPDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2052 HSP 13 Score: 75.485 bits (184), Expect = 4.924e-12 Identity = 63/198 (31.82%), Postives = 91/198 (45.96%), Query Frame = 0
Query: 919 SAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQD-DDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRAS 1115
S T S TP + E NT +PS LP T + P LP P + L S V A V+ + E A ERT+ + K S +LG+ V +G+ G++VRS+ GG +SRDGRI GD ++++N E +++QA +LRR +I I+ITY+PA +R S
Sbjct: 918 SGFTVSDYTPANAVEQKYECANTVAWTPS----QLPSGLSTTELAPALPAVAP------------KYLTEQSSLVSDAESVTLQSMSQE---------AFERTVTIAKGSSSLGMTVSANKDGL-GVIVRSIIHGGAISRDGRIAVGDCILSINEESTISLTNAQARAMLRRHSLI--GPDIKITYVPAEHLEEFRVS 1087 HSP 14 Score: 65.0846 bits (157), Expect = 8.049e-9 Identity = 57/217 (26.27%), Postives = 100/217 (46.08%), Query Frame = 0
Query: 1590 VSLNKG-TNGLGISLAGH--KDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGL-----------HEVSILKGEQGLGIMIIEGK---HADVGSGVFVSD-LQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIV 1788
V + KG + LG+S+AG + ++I +HP+G A + +++VGD I+ + G H + +KN S ++ D S+ + L+ NP ++G +++ +G GLG I+ G H D+ ++V G+ A+ L+RGD I+AVNG+ GV +E A ILK ++G + ++V
Sbjct: 1847 VEIKKGPADALGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNASGSIEVQVVAGGDVSVVTGHQQELA---------NPCLAFTGLTSSTIFPDDLGPPQSKTITLDRGPDGLGFSIVGGYGSPHGDLP--IYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2052 HSP 15 Score: 63.929 bits (154), Expect = 1.819e-8 Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 0
Query: 1922 PMGLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI 1994
P GLG SIVGG + G I++ V+ KGAA +DGRLK GDQI+ V + L V+H A+ L++T V LM+
Sbjct: 1978 PDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMV 2052 HSP 16 Score: 63.5438 bits (153), Expect = 2.302e-8 Identity = 54/221 (24.43%), Postives = 102/221 (46.15%), Query Frame = 0
Query: 1575 KILKKYARAKGTPIYVSLNKGTNGLGISLAGHK--DRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDR-HHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSG--KYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVA 1789
+++K A+ + I+ L GLG S+ G + +R ++ +++ + A RD R++ D IL +NG V+ H S ++ D+ + ++ R +L + P + + +S ++ + + ++ GLG II GK +GV V + PG AD G L GD IL + D G++ E A++L+ +K+++A
Sbjct: 125 QLIKSMAQGRHVEIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDT-IQLVIARG-----SLPHISSPRISRSPSAASTVSAHSNPTHWQHVETIELVNDGSGLGFGIIGGK----ATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIA 335
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|42559535|sp|Q9NB04.2|PATJ_DROME (RecName: Full=Patj homolog) HSP 1 Score: 318.546 bits (815), Expect = 3.636e-90 Identity = 182/351 (51.85%), Postives = 235/351 (66.95%), Query Frame = 0
Query: 629 PEIFSETSEEY---WKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFP-LGGTFRHEILANTERMVKAKSDVSISSAGTVTE----SSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPP 971
PE+ + E W+ +GS +++A I+K GGLGISLEGTV+ G E PHHYIRS+LP+GPVG NGVL+SGDELLEVNG +LLG+ H +VVAILK+LP+ V++VC R+++ S + L F + +L T+R+VKAKSD S+++AG+V + ++ SKLKSRSLEPLT LAMWS IEL+KG+RGLGFSILDYQDP++ ++T+IVIRSLVPGGV+Q DGRLIPGDRL++VN INLE+ASLD+AVQALKGA +G+VRIGV KPLP+ D+S S N+S + E + D P PP LPT PP
Sbjct: 533 PELSDDAETEIIRKWQKIVGSDVEVIVAQIKKFA-VGGLGISLEGTVDVEGGREVRPHHYIRSILPDGPVGVNGVLRSGDELLEVNGERLLGMNHLEVVAILKELPLDVRMVCGRNRNSSLLPFSDDTLKKLSNNFEN-LLPATDRLVKAKSDGSLATAGSVADGDSVAAAAASFSKLKSRSLEPLTGLAMWSSQPQIIELVKGDRGLGFSILDYQDPLDPNDTLIVIRSLVPGGVAQLDGRLIPGDRLLFVNSINLENASLDQAVQALKGASKGVVRIGVAKPLPM--------TDNSLKACS----------NASTTSEETL------------DAQPSPPALPTVAPP 851 HSP 2 Score: 244.973 bits (624), Expect = 1.152e-65 Identity = 144/309 (46.60%), Postives = 194/309 (62.78%), Query Frame = 0
Query: 263 SDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVD---PSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIA 568
+D+ Q++ IEL N+G G GFG++ G + +GV+VKT++ GVAD+DGRL +GD+ILQI D L EQVA VLR+SG HVRL+VAR V+ P+ ++P ++P+ +L AELE +L I+ F +++ P T + + +T + + ++D EELL +PE E L VEL KDA GLGITIAGY CE+EELSGIFVKSV+ GSAA SG + VND+IIEVDG+S+QG+SN QAVE+L+ +G+ V LRL RY+ G KFEQLQQAIA
Sbjct: 142 TDYAQIQAIELVNDGTGLGFGII-GARNSGVIVKTILPGGVADKDGRLRSGDHILQIGDVNLHEMVSEQVAAVLRQSGTHVRLVVARPVEQSVPTPQYALEPGTAVVPTRVLVDPAELERYL-ISTGYPEIFGESSTASTPQTTTEDDRFVYRGETSML-------IDPNIDL------EELLA-------------------LPETEKLQVELKKDANGLGITIAGYVCEKEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLKKSGQVVNLRLERYLRGPKFEQLQQAIA 416 HSP 3 Score: 117.857 bits (294), Expect = 4.108e-25 Identity = 112/400 (28.00%), Postives = 178/400 (44.50%), Query Frame = 0
Query: 41 QDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQAL--------ASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQ--ASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSP--PETTALPSR--------------------------ESTPRVLNSD--------------------------WCQVEVIELTNNGAGFGFG----VMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPI---LPSS 369
+ L+T++ +L+ PVF+ I+++QDSL EL Q+ HPS+LP+DFDI V G+L+L+L G+ + +S HS AP SP PR +++ A + A+ ++ I L +G LGF ++G ++ G+ V+ I P G+A +DG+L GD IL I L +S Q +L+++ V LVVAR + + Q P T +P+R STP+ D + +EL + G G V ++ +G+ VK+V AD GR+ D I++++ LQG+ Q +L+KSG V L + R + Q++ I LPSS
Sbjct: 35 ESLSTILGILQDPVFRTIVHVQDSLSELNAQLAQHPSMLPNDFDIDVAGNLVLSLNGGEVMYDFDEQRSSSHSHS---APGSPDKSGGVGEEPRPQSQNSKGAGVADLYATDYAQIQAIELVN-DGTGLGFGIIGARNS-----GVIVKTILPGGVADKDGRLRSGDHILQIGDVNLHEMVS-EQVAAVLRQSGTHVRLVVARPVEQSVPTPQYALEPGTAVVPTRVLVDPAELERYLISTGYPEIFGESSTASTPQTTTEDDRFVYRGETSMLIDPNIDLEELLALPETEKLQVELKKDANGLGITIAGYVCEKEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLKKSGQVVNLRLERYLRGPKFEQLQQAIAANDKLPSS 424 HSP 4 Score: 77.411 bits (189), Expect = 9.261e-13 Identity = 71/278 (25.54%), Postives = 133/278 (47.84%), Query Frame = 0
Query: 819 IELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMI 1093
IEL+ GLGF I+ ++ + +++++++PGGV+ +DGRL GD ++ + D+NL H + E V A+ VR+ V +P+ + QY + + D E+ ER S P++ + T P T ED F R G TS + +P + + + LP + + ++KD++ LGI + + E ++G+ V+SV+ G GRI D ++ V+G+ ++ S+ QA+++L+++ +
Sbjct: 150 IELVNDGTGLGFGIIGARN------SGVIVKTILPGGVADKDGRLRSGDHILQIGDVNL-HEMVSEQVAAVLRQSGTHVRLVVARPVEQSVPTPQYALEPGTAVVPTRVLVDPAEL-------ERYLISTGYPEIFGESSTASTPQTTT----EDDRFVYR------GETSMLI---------DPNIDLEELLALPETEKLQVELKKDANGLGITIAGYVCEKEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLKKSGQV 394 HSP 5 Score: 60.077 bits (144), Expect = 1.929e-7 Identity = 73/271 (26.94%), Postives = 119/271 (43.91%), Query Frame = 0
Query: 641 KNQLGSGHAILMAT-------IQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEIL---ANTERMVKAKSDVSI-------SSAGTVTESS---HQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDE-TVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALK 890
+N G+G A L AT I+ GLG + G +N ++++LP G +G L+SGD +L++ L + V A+L+ V+LV AR QS P +L A ER + + I S+ T TE ++ + S L +++ LA+ L++EL K GLG +I Y K+E + I ++S+ PG + GR+ DR++ V+ +L+ S +AV+ LK
Sbjct: 129 QNSKGAGVADLYATDYAQIQAIELVNDGTGLGFGIIGA--------RNSGVIVKTILPGGVADKDGRLRSGDHILQIGDVNLHEMVSEQVAAVLRQSGTHVRLVVARPVEQSVPTPQYALEPGTAVVPTRVLVDPAELERYLISTGYPEIFGESSTASTPQTTTEDDRFVYRGETSMLIDPNIDLEELLALPETEKLQVELKKDANGLGITIAGYV--CEKEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLK 389 HSP 6 Score: 60.077 bits (144), Expect = 2.172e-7 Identity = 73/290 (25.17%), Postives = 124/290 (42.76%), Query Frame = 0
Query: 471 VELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSV-SQVAVSATPLAR-----------------SSPQGV--DNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR 740
+ELV D GLG I G SG+ VK++ G A K G + D I+++ ++ ++Q +LR +G VRL + R V +Q++ Q + T V ++V V L R S+PQ D+ +GE+ S L + + L++L PE +E+ ++ +K GLGI++ G V + +E+ +++SV P +G ++ D ++EV+G+ L G + V +LK V L R
Sbjct: 150 IELVNDGTGLGFGIIGARN-----SGVIVKTILPGGVADKDGRLRSGDHILQIGDVNLHEMVSEQVAAVLRQSGTHVRLVVARPV--------EQSVPTPQYALEPGTAVVPTRVLVDPAELERYLISTGYPEIFGESSTASTPQTTTEDDRFVYRGET---SMLIDPNIDLEELLALPE-----TEKL--------------QVELKKDANGLGITIAGYVCE---KEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLKKSGQVVNLRLER 401 HSP 7 Score: 58.5362 bits (140), Expect = 6.420e-7 Identity = 39/114 (34.21%), Postives = 59/114 (51.75%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVG----KKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPG 2112
E L VEL KK GLG++I G K+ G+F+ V G AAD GR+ D+II V+G L S A +LK + + +R+ R G + + + + + +PS+ PG
Sbjct: 315 EKLQVEL-KKDANGLGITIAGYVCEKEELSGIFVKSVSPGSAADLSGRIRVNDRIIEVDGQSLQGYSNHQAVELLKKSGQVVNLRLERYLRGPKFEQLQQAIAANDKLPSSAPG 427
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|116242542|sp|Q8NI35.3|INADL_HUMAN (RecName: Full=InaD-like protein; Short=Inadl protein; Short=hINADL; AltName: Full=Pals1-associated tight junction protein; AltName: Full=Protein associated to tight junctions) HSP 1 Score: 304.679 bits (779), Expect = 5.776e-82 Identity = 213/561 (37.97%), Postives = 295/561 (52.58%), Query Frame = 0
Query: 1698 ERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQR-GDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAI---------------TAPVSPP----APVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQG--------VGGMHSRDSSIPSNVPGATG-----GTM----------------KTVELKRG-QHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
+RY+ LH + + K + GLG+ + K S +FV + P A G R GD +L +N + G ++++A+ I+K + +K++ V + + T P+S V + P++ES K P + S I P+ P AS ++ A + + P+ S +PATC I PG + +IEISK + GLGLSIVGG DT L AI IHEVYE+GAA +DGRL GDQILEV DLRN SH A+ ALRQTP +VRL+++R+ D+E E V+L KK RGLGLSIVGK++G GVFIS++VKGGAAD DGRL+QGDQI++VNG D+ N+SQE A ILK QG + + + RLR G+ + S PS P TG GT +TVE+ R LG SI GG GSP GD+P+++ + +G AA LK GD+I+S+N +PL+GLSH D VN+LKN G ++LQ+++
Sbjct: 1228 QRYADLPGELHIIELEKDKNGLGLSLAGNKDRSRMS-IFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPSKVKLVFIRNEDAVNQMAVTPFPVPSSSPSSIEDQSGTEPISSEEDGSVEVGIKQLPESESFKLAVSQMKQQKYPTKVSFSSQEI-PLAP--------ASSYHSTDADF--TGYGGFQAPL----------SVDPATCPIVPGQEMIIEISKGRS------GLGLSIVGGKDTPLNAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEEAITALRQTPQKVRLVVYRDEAHYRDEENLEIFPVDLQKKAGRGLGLSIVGKRNGSGVFISDIVKGGAADLDGRLIQGDQILSVNGEDMRNASQETVATILKCAQGLVQLEIGRLRAGSWTSARTTSQNSQGSQQSAHSSCHPSFAPVITGLQNLVGTKRVSDPSQKNSGTDMEPRTVEINRELSDALGISIAGGRGSPLGDIPVFIAMIQASGVAARTQKLKVGDRIVSINGQPLDGLSHADVVNLLKNAYGRIILQVVA 1760 HSP 2 Score: 235.343 bits (599), Expect = 1.019e-60 Identity = 180/503 (35.79%), Postives = 257/503 (51.09%), Query Frame = 0
Query: 466 METLDVELV-KDAQGLGITIAGY--TCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVS-ATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQD--------LENQPEIFSETSEEYWKNQLGSGHAILMATIQKQ-------------KPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSS--DVNT 941
ET +VELV KD Q LGI I GY T E SGI+VKS+ GSAA+ +G + VND+I+ VDG +IQGF+N VE+LR+ G+ V L LVR S S S PS V P A V+ E + GE D E+ E ++L++ LE P+ + W+N LG + +++AT+ Q P L + +E V+ DG HHYI S++ GPV G+L+ DELLEVNG +L G + V+ LK++P LVC R F E + R +++ S+ TE H + + E LA+WS + +EL+K +GLGFSILDYQDP++ +VIVIRSLV GV+++ G L+PGDRL+ VN+ L++ SL EAV+ LK P G+V +G+ KPL + +E++S + SS+ D TE + + D+N+
Sbjct: 361 FETYNVELVRKDGQSLGIRIVGYVGTSHTGEASGIYVKSIIPGSAAYHNGHIQVNDKIVAVDGVNIQGFANHDVVEVLRNAGQVVHLTLVRR-------------KTSSSTSPLEPPSDRGTVVEPLKPPALFLTGAVETETNVDGE---DEEIKERIDTLKNDNIQALEKLEKVPDSPENELKSRWENLLGPDYEVMVATLDTQIADDAELQKYSKLLPIHTLRLGVE--VDSFDG-----HHYISSIVSGGPVDTLGLLQPEDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRR-----------------LFDDEASVDEPR----RTETSLPE----TEVDHNMDVNTEEDDDGE----LALWSPEVKIVELVKDCKGLGFSILDYQDPLDPTRSVIVIRSLVADGVAERSGGLLPGDRLVSVNEYCLDNTSLAEAVEILKAVPPGLVHLGICKPLV------EDNEEESCYILHSSSNEDKTEFSGTIHDINS 805 HSP 3 Score: 226.098 bits (575), Expect = 6.838e-58 Identity = 131/310 (42.26%), Postives = 186/310 (60.00%), Query Frame = 0
Query: 41 QDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVARE 350
+ L+ L+SP+F IL +Q S+ +LK Q+ PS ++FD G L+ DG VH P+ S S L P+L NE F ++IQQ +QGR +++I + +P LGFSVV L+S++ G++ I+V+++QP +A +D +L E DQILAI+ PLD NISH QAI +LQ+ G + L+VAR ST S T LP W VE +EL N+G+G GFG++ G + +GVVV+T+V G+AD+DGRL GD+IL+I +QG EQVA VLR GN VR++VAR+
Sbjct: 33 EKLSMFYETLKSPLFNQILTLQQSIKQLKGQLNHIPSDCSANFDFSRKGLLVFT--DGSITNGNVHR--PSNNSTVSGL-FPWTPKLGNED-FNSVIQQMAQGRQIEYIDIERPSTGGLGFSVVALRSQNLGKVDIFVKDVQPGSVADRDQRLKENDQILAINHTPLDQNISHQQAIALLQQTTGSLRLIVAREPVHTKSSTSSSLNDTTLPETVC--------WGHVEEVELINDGSGLGFGIVGG-KTSGVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARD 327 HSP 4 Score: 124.405 bits (311), Expect = 7.110e-27 Identity = 101/309 (32.69%), Postives = 152/309 (49.19%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGA--IFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHRE-------------DDEIYETL------DVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGN---RKQGVGGMHSRDSSIPSNVPGATGGTMKT--VELKRGQ-HGLGFSIVGGFGSPH-GDMP-IYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
GLG S+V LG IF+ +V A +D RLK DQIL + H L +N+SH A+ L+QT +RL++ RE D + ET+ +VEL GLG IVG K+ GV + +V GG AD DGRL GD I+ + G ++ + E A +L+ + M V R G+ + ++ S PG+ +T VEL R LG IVG G+ H G+ IYVK++ AA +G ++ D+I++V+ ++G ++HD V +L+N V L ++
Sbjct: 144 GLGFSVVALRSQNLGKVDIFVKDVQPGSVADRDQRLKENDQILAINHTPLDQNISHQQAIALLQQTTGSLRLIVAREPVHTKSSTSSSLNDTTLPETVCWGHVEEVEL-INDGSGLGFGIVGGKTS-GVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARDPAGDISVTPPAPAALPVALPTVASKGPGSDSSLFETYNVELVRKDGQSLGIRIVGYVGTSHTGEASGIYVKSIIPGSAAYHNGHIQVNDKIVAVDGVNIQGFANHDVVEVLRNAGQVVHLTLV 450 HSP 5 Score: 106.686 bits (265), Expect = 1.656e-21 Identity = 123/475 (25.89%), Postives = 200/475 (42.11%), Query Frame = 0
Query: 480 LGITIAG--YTCER----EELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAIL---MATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ----------------------------------STSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSR-------SLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQD-PMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTK 903
LGI+I G +R EEL GIF+K V + S A K+ + D+I+EV G +Q S+ +AVE +++ G V I S S++ P+V A T Q D + + G + + L +E + + ++ +A L + I+ +K GLG+SL G ++ ++ + P GP A+G ++ GDELLE+N + L G H + AI+K P VKLV R++ S VG KQ P +F+ + ++ K S SS+ + S++P T + ++ IE+ KG GLG SI+ +D P+N IVI + G + +DGRL GD+++ VN ++L ++S +EA+ AL+ P+ VR+ V +
Sbjct: 1079 LGISIVGGQTVIKRLKNGEELKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHSEAVEAIKNAGNPVVF-----------------IVQSLSSTPRVIPNVHNKANKIT-----GNQNQDTQEKKEKRQGTAPPPMKLPPPYKAL-TDDSDENEEEDAFTDQKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKDR-----SRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPSKVKLVFIRNEDAVNQMAVTPFPVPSSSPSSIEDQSGTEPISSEEDGSVEVGIKQ-LPESESFKLAVSQMKQQKYPTKVSFSSQEIPLAPASSYHSTDADFTGYGGFQAPLSVDPATCPIVPGQEMI-IEISKGRSGLGLSIVGGKDTPLN----AIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEEAITALRQTPQ-KVRLVVYR 1518 HSP 6 Score: 103.99 bits (258), Expect = 1.304e-20 Identity = 46/112 (41.07%), Postives = 75/112 (66.96%), Query Frame = 0
Query: 1570 GYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAV 1681
++ +KI ++YA G + L K NGLG+SLAG+KDRS+MS+++ G++P G A D RM++GD +LE+N ++Y R H N S+ IK P S V + +R++ +++ +AV
Sbjct: 1220 AFTDQKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKDRSRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAP-SKVKLVFIRNEDAVNQMAV 1330 HSP 7 Score: 95.1301 bits (235), Expect = 5.939e-18 Identity = 53/105 (50.48%), Postives = 66/105 (62.86%), Query Frame = 0
Query: 1342 NWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSL 1446
+WGP R V I R PN LGISIVGG+ K+G + GIFIK VLE SPAG ++ L TGD+IL V G DL A+H +AV+ I+ +G V F+VQSL
Sbjct: 1062 HWGPPRIVEIFREPNVSLGISIVGGQTVIKRLKNGEE-------LKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHSEAVEAIKNAGNPVVFIVQSL 1159 HSP 8 Score: 94.7449 bits (234), Expect = 6.796e-18 Identity = 66/206 (32.04%), Postives = 107/206 (51.94%), Query Frame = 0
Query: 1585 GTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSI---DNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKM 1786
G + + ++KG +GLG+S+ G KD ++ I ++ G A RD R+ GD ILEVNG + + H + ++ P V ++ R ++ +NL + FP + K K +GLG+ I+ ++ GSGVF+SD+ G AD+ G L +GD IL+VNGED + E+ A ILK ++GL+++
Sbjct: 1433 GQEMIIEISKGRSGLGLSIVGGKDTPLNAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEEAITALRQTPQK-VRLVVYRDEAHYRDEENLEI-----FPVDLQK--------------------KAGRGLGLSIVGKRN---GSGVFISDIVKGGAADLDGRLIQGDQILSVNGEDMRNASQETVATILKCAQGLVQL 1609 HSP 9 Score: 82.0333 bits (201), Expect = 5.184e-14 Identity = 67/219 (30.59%), Postives = 101/219 (46.12%), Query Frame = 0
Query: 134 QALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSA--EDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
Q +I + S+GR LG S+VG K I + E+ +G AA+DG+L GDQIL ++G L N SH +AI L++ +V LVV R A D E+ + P ++L G G G ++ + +GV + +V+ G AD DGRL GD IL +N ++ E VA +L+ + V+L + R
Sbjct: 1434 QEMIIEISKGRS------------GLGLSIVGGKDTPLN--AIVIHEVYEEGAAARDGRLWAGDQILEVNGVDL-RNSSHEEAITALRQTPQKVRLVVYRDEAHYRDEENLEIFP------------------------VDLQKKAGRGLGLSIVGKRNGSGVFISDIVKGGAADLDGRLIQGDQILSVNGEDMRNASQETVATILKCAQGLVQLEIGR 1613 HSP 10 Score: 78.5666 bits (192), Expect = 5.706e-13 Identity = 112/466 (24.03%), Postives = 187/466 (40.13%), Query Frame = 0
Query: 658 QKPNGGLGISLEG---TVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLE------------IELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGV-----TKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERM--SPSVNL----------PDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQ-VDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRT 1090
++PN LGIS+ G ++++ E+ +I+ VL + P G LK+GD++LEV+G L H++ V +K+ V + QS S+ T + P ++I N + + K + GT P K + + ++D + IEL K + GLG S+ +D + I + + P G + DGR+ GD L+ +N+ L S A +K AP + + + + + S +S + P + E S +V +++ S S L P IP +S D DF T G A P+ P+ E I + K LG+ V +D +N +V+ V G +RDGR++ GD ++ VNG +R +SH +A+ LR+T
Sbjct: 1073 REPNVSLGISIVGGQTVIKRLKNGEELKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHSEAVEAIKNAGNPVVFIV---QSLSS---TPRVIPNVHNKANKITGNQNQDTQEKKE---KRQGTAPPPMKLPPPYKALTDDSDENEEEDAFTDQKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKD---RSRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPSKVKLVFIRNEDAVNQMAVTPFPVPSSSPSSIEDQSGTEPISSEEDGSVEVGIKQLPESESFKLAVSQMKQQKYPTKVSFSSQEIPLAPASSYHSTDADF-----------TGYGGFQA-------PLSVDPATCPIVPGQEMIIEISKGRSGLGLSIVGGKDTPLNAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEEAITALRQT 1508 HSP 11 Score: 71.633 bits (174), Expect = 6.850e-11 Identity = 98/405 (24.20%), Postives = 163/405 (40.25%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEV--IELT-NNGAGFGF------GVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHI-------PILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQT-EQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSG-IFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLR 541
+G LGF +VG K+ G+ V+ I P G+A +DG+L GD IL I G + ++ Q +L+ V ++VAR A D T P + + SD E +EL +G G G +A+G+ VK+++ A +G + D I+ ++ +QGF V VLR +G V L + R S S ++P P+ P ++ L AV ++ + L ND I + E+V + L + P E +M T +++ D EL K ++ L I E + G ++ S+ G G++ D+++EV+G + G S ++AV L+
Sbjct: 254 DGSGLGFGIVGGKTS-----GVVVRTIVPGGLADRDGRLQTGDHILKIGGTNV-QGMTSEQVAQVLRNCGNSVRMLVARDPAGDISVTPPAPAALPVALPTVASKGPGSDSSLFETYNVELVRKDGQSLGIRIVGYVGTSHTGEASGIYVKSIIPGSAAYHNGHIQVNDKIVAVDGVNIQGFANHDVVEVLRNAGQVVHLTLVRRKTSSSTSPLEPPSDRGTVVEPLKPPALF---------LTGAVETETNVDGEDEEIKERIDTLKNDNIQALEKLEKVPDSPENELKSRWENLLGPDYE--VMVATLDTQIAD----------------DAELQKYSKLLPIHTLRLGVEVDSFDGHHYISSIVSGGPVDTLGLLQPEDELLEVNGMQLYGKSRREAVSFLK 625 HSP 12 Score: 60.4622 bits (145), Expect = 2.131e-7 Identity = 63/229 (27.51%), Postives = 109/229 (47.60%), Query Frame = 0
Query: 1583 AKGTPI-YVSLNK-GTNGLGISLAGHKDRS--KMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRH--HLNVSSFIKNLPDSDVTFILLR--------SDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVA-NPIGPV 1795
A+G I Y+ + + T GLG S+ + ++ K+ +++ + P A RD R++ D IL +N H D++ H + ++ S + I+ R + SS+++ + PE + + + EV ++ GLG I+ GK SGV V + PG AD G LQ GD IL + G + G+ E A++L+N ++M+VA +P G +
Sbjct: 127 AQGRQIEYIDIERPSTGGLGFSVVALRSQNLGKVDIFVKDVQPGSVADRDQRLKENDQILAIN-HTPLDQNISHQQAIALLQQTTGS-LRLIVAREPVHTKSSTSSSLNDTTL-------PE----------TVCWGHVEEVELINDGSGLGFGIVGGK----TSGVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARDPAGDI 332 HSP 13 Score: 60.4622 bits (145), Expect = 2.149e-7 Identity = 67/242 (27.69%), Postives = 106/242 (43.80%), Query Frame = 0
Query: 149 IALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR---GSAEDSESTQS-----------------PPETTALPSRESTPRVLNSDWCQ-----------VEV-IELTNNGAGFGFGVMAGQQA----AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS 354
+ L K G LG S+VG ++ G+++ +I G A DG+LI+GDQIL+++G+ + N S IL+ A+G V L + R GS + +T P T L + T RV SD Q VE+ EL++ G + G+ + V + + +GVA + +L GD I+ IN L G V N+L+ + + L V + + S
Sbjct: 1534 VDLQKKAGRGLGLSIVGKRNGS----GVFISDIVKGGAADLDGRLIQGDQILSVNGEDM-RNASQETVATILKCAQGLVQLEIGRLRAGSWTSARTTSQNSQGSQQSAHSSCHPSFAPVITGLQNLVGTKRV--SDPSQKNSGTDMEPRTVEINRELSD---ALGISIAGGRGSPLGDIPVFIAMIQASGVAARTQKLKVGDRIVSINGQPLDGLSHADVVNLLKNAYGRIILQVVADTNIS 1765 HSP 14 Score: 58.9214 bits (141), Expect = 5.467e-7 Identity = 32/96 (33.33%), Postives = 59/96 (61.46%), Query Frame = 0
Query: 139 QASQGRDV--KHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVA 232
Q + G D+ + + +++ +LG S+ G + G++ +++ IQ G+AA+ +L GD+I++I+GQPLD +SH +N+L+ A GR+ L V
Sbjct: 1665 QKNSGTDMEPRTVEINRELSDALGISIAGGRGSPLGDIPVFIAMIQASGVAARTQKLKVGDRIVSINGQPLDG-LSHADVVNLLKNAYGRIILQVV 1759
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|68052319|sp|Q63ZW7.2|INADL_MOUSE (RecName: Full=InaD-like protein; Short=Inadl protein; AltName: Full=Channel-interacting PDZ domain-containing protein; AltName: Full=Pals1-associated tight junction protein; AltName: Full=Protein associated to tight junctions) HSP 1 Score: 239.965 bits (611), Expect = 4.512e-62 Identity = 184/504 (36.51%), Postives = 258/504 (51.19%), Query Frame = 0
Query: 462 DMPEMETLDVELVK-DAQGLGITIAGY--TCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETS--------EEYWKNQLGSGHAILMATIQKQ-------------KPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTES---SSTPYDNT-EVNSS 937
D ET VELVK D Q LGI I GY T E SGI+VKS+ GSAA+ +G + VND+I+ VDG +IQGF+NQ VE+LR+ G+ V L LVR L + + S + A P V + A S P A S + E G+ + + ++Q LE +P+++ E + W+N LG + +++AT+ Q P L + +E V+ DG HHYI SV P GPV +L+ DELLEVNG +L G + V+ LK++P LVC R + D P R A E V DV+I LA+WS + +EL+K +GLGFSILDYQDP++ +VIVIRSLV GV+++ G L+PGDRL+ VN+ +L++A+L EAV+ LK P G+V +G+ KPL V ++ +E S H+ + SS P D E++SS
Sbjct: 357 DRSPFETYSVELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVAVDGVNIQGFANQDVVEVLRNAGQVVHLTLVRRKTSLSASPFEHS---SSRETVAEPPKVPERAGSPKPEANLSVE---------AEEIGERLDNLKNNTVQALE-KPDVYPEKVPGSPENELKSRWENLLGPDYEVMVATLDAQIADDEELQKYSKLLPIHTLRLGME--VDSFDG-----HHYISSVAPGGPVDTLNLLQPEDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRRLFDDEA---SVDEP-----RTMEPALLEAEVDHSVDVNIEDDDD---------------------GELALWSPEVKTVELVKDCKGLGFSILDYQDPLDPTRSVIVIRSLVADGVAERSGELLPGDRLVSVNEFSLDNATLAEAVEVLKAVPPGVVHLGICKPL----VEDEKEERFSLHSNNNGDSSEPADAVHEIHSS 807 HSP 2 Score: 216.083 bits (549), Expect = 9.503e-55 Identity = 136/344 (39.53%), Postives = 194/344 (56.40%), Query Frame = 0
Query: 36 EVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPI-LPSSMLA 372
+ T + L+ L+SP+F IL +Q S+ +LK Q+ PS ++FD G L+ DG S + + P S + E + F ++IQQ +QGR V++I + +P LGFSVV L+S+ G + I+V+E+ P +A +D +L E DQILAI+ PLD NISH QAI +LQ+A G + LVVAR G A S + + PET W E +EL N+G+G GFG++ G +++GVVV+T+V G+AD+DGRL GD+IL+I +QG EQVA VLR GN VR++VAR DP V P P+ LP ++ A
Sbjct: 28 DTTQNEKLSAFYETLKSPLFNQILTLQQSIKQLKGQLSHIPSDCSANFDFSRKGLLVFT--DG----SITNGNAQRPCSNVTASELLPWTQKSASEDFNSVIQQMAQGRHVEYIDIERPSTGGLGFSVVALRSQSLGLIDIFVKEVHPGSVADRDHRLKENDQILAINDTPLDQNISHQQAIALLQQATGSLRLVVAREVGHTQGRASTSSADTTLPETVC--------------WGHTEEVELINDGSGLGFGIVGG-KSSGVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVAR--DPVGEIAVTPPTPVSLPVALPA 348 HSP 3 Score: 120.553 bits (301), Expect = 1.205e-25 Identity = 101/313 (32.27%), Postives = 151/313 (48.24%), Query Frame = 0
Query: 1924 GLGLSIVGGSDTLLGAI--FIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHRE-------------DDEIYETL------DVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMK---------TVEL-KRGQHGLGFSIVGGFGSPH-GDMP-IYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
GLG S+V LG I F+ EV+ A +D RLK DQIL + L +N+SH A+ L+Q +RL++ RE D + ET+ +VEL GLG IVG KS GV + +V GG AD DGRL GD I+ + G ++ + E A +L+ + M V R VG + S+P +P T+ +VEL K+ LG IVG G+ H G+ IYVK++ AA +G ++ D+I++V+ ++G ++ D V +L+N V L ++
Sbjct: 144 GLGFSVVALRSQSLGLIDIFVKEVHPGSVADRDHRLKENDQILAINDTPLDQNISHQQAIALLQQATGSLRLVVAREVGHTQGRASTSSADTTLPETVCWGHTEEVEL-INDGSGLGFGIVGGKSS-GVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARDPVGE----IAVTPPTPVSLPVALPAVATRTLDSDRSPFETYSVELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVAVDGVNIQGFANQDVVEVLRNAGQVVHLTLV 450 HSP 4 Score: 117.472 bits (293), Expect = 1.016e-24 Identity = 58/146 (39.73%), Postives = 89/146 (60.96%), Query Frame = 0
Query: 1557 PKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSG 1702
P + EG EED + +KI ++YA G + L K NGLG+SLAG+KDRS+MS+++ G++P G A D RM++GD +LE+N ++Y R H N S+ IK P + V + +R++ ++ +AV P FP H +P+E G
Sbjct: 1214 PSADMEGSEED-CALTDKKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKDRSRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAP-TRVKLVFIRNEDAVSQMAVAP---FPELSHSPSPVEDLGG 1354 HSP 5 Score: 106.686 bits (265), Expect = 1.532e-21 Identity = 114/418 (27.27%), Postives = 186/418 (44.50%), Query Frame = 0
Query: 1585 GTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSI---DNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKL--PPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI 1994
G + + ++KG +GLG+S+ G KD ++ I ++ G A RD R+ GD ILEVNG + H + ++ P V ++ R ++ +NL V + +++ +G+ GLG+ I+ GK + GSGVF+SD+ G AD+ G L RGD IL+VNGED + E+ A ILK +GL+++ IG +++ S +A T Q D+ +P+ AP V+ S V + ++ + P +K +E P T EI + E+S LG+SI GG + LG IFI + G A + +LK GD+I+ + + L +SH+ A++ L+ R+ L +
Sbjct: 1468 GQEMIIEISKGRSGLGLSIVGGKDTPLDAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRSSSHEEAITALRQTPQK-VRLVVYRDEAQYRDEENLEVFLVD-----------LQKKTGR--------------GLGLSIV-GKRS--GSGVFISDIVKGGAADLDGRLIRGDQILSVNGEDMRHASQETVATILKCVQGLVQL----EIGRLRAGSWAASR------------KTSQNSQGDQHSAHSSCRPSFAP--------------------------VITSLQNLVGTKRS------SDPPQKCTEE--EPRTVEI------IRELSD---------ALGISIAGGKGSPLGDIPIFIAMIQANGVAARTQKLKVGDRIVSINGQPLDGLSHTDAVNLLKNAFGRIILQV 1791 HSP 6 Score: 94.7449 bits (234), Expect = 6.496e-18 Identity = 54/105 (51.43%), Postives = 66/105 (62.86%), Query Frame = 0
Query: 1342 NWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSL 1446
+WGP R V I R PN LGISIVGG+ K+G + GIFIK VLE SPAG ++ L TGD+IL V G DL A+H +AV+ I+ +G V FVVQSL
Sbjct: 1068 HWGPPRIVEIFREPNVSLGISIVGGQTVIKRLKNGEE-------LKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHAEAVEAIKSAGNPVVFVVQSL 1165 HSP 7 Score: 81.6481 bits (200), Expect = 6.815e-14 Identity = 65/217 (29.95%), Postives = 102/217 (47.00%), Query Frame = 0
Query: 134 QALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIEL-TNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
Q +I + S+GR LG S+VG K I + E+ +G AA+DG+L GDQIL ++G L S+ SH +AI L++ +V LVV R A+ + + +V +++L G G G ++ + +GV + +V+ G AD DGRL GD IL +N ++ E VA +L+ V+L + R
Sbjct: 1469 QEMIIEISKGRS------------GLGLSIVGGKDTPLD--AIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRSS-SHEEAITALRQTPQKVRLVVYRDEAQ----------------------YRDEENLEVFLVDLQKKTGRGLGLSIVGKRSGSGVFISDIVKGGAADLDGRLIRGDQILSVNGEDMRHASQETVATILKCVQGLVQLEIGR 1648 HSP 8 Score: 81.6481 bits (200), Expect = 7.732e-14 Identity = 81/285 (28.42%), Postives = 127/285 (44.56%), Query Frame = 0
Query: 471 VELVKDAQ-GLGITIAG--YTCER----EELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEE---YWKNQLGSGHAIL---MATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQ 742
VE+ ++ LGI+I G +R EEL GIF+K V + S A K+ + D+I+EV G +Q S+ +AVE ++S G V + S S++ P+V+ TP +P N + K + G + P E SEE ++ +A L + I+ +K GLG+SL G ++ ++ + P GP A+G ++ GDELLE+N + L G H + AI+K P VKLV R++
Sbjct: 1075 VEIFREPNVSLGISIVGGQTVIKRLKNGEELKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHAEAVEAIKSAGNPVVF-----------------VVQSLSSTPRVIPTVNNKG--KTP----APNQDQNTQERKAKRHGTAPPPMKLPPPY---RAPSADMEGSEEDCALTDKKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKDR-----SRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPTRVKLVFIRNE 1328 HSP 9 Score: 80.1073 bits (196), Expect = 2.035e-13 Identity = 138/597 (23.12%), Postives = 230/597 (38.53%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSR--ESTPRVLNSDWCQVEV--IELTN-NGAGFGF------GVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVARE--------VDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSG-IFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLP 731
+G LGF +VG KS G+ V+ I P G+A +DG+L GD IL I G + ++ Q +L+ V ++VAR E +PP +LP R L+SD E +EL +G G G +A+G+ VK+++ A +G++ D I+ ++ +QGF + V VLR +G V L + R + S + + P +P + + E ++ + L NL N+ + E+ +Y P +P S + E LL + ++ + + D EL K ++ L I E + G ++ SV G ++ D+++EV+G + G S ++AV L+ L R + F+ S + P++ + VD+ VD E D EL+ S PE+ T++ K GLG S+ + +D IRS++ +G +G L GD L+ VN L + V +LK +P
Sbjct: 254 DGSGLGFGIVGGKSS-----GVVVRTIVPGGLADRDGRLQTGDHILKIGGTNV-QGMTSEQVAQVLRNCGNSVRMLVARDPV--GEIAVTPPTPVSLPVALPAVATRTLDSDRSPFETYSVELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVAVDGVNIQGFANQDVVEVLRNAGQVVHLTLVRRKTSLSASPFEHSSSRETVAEPPKVPERAGSPKPEANLSVEAEEIGER------------LDNLKNNTV--QALEKPDVY-PEKVPGSPENELKSRWENLL------GPDYEVMVATLDAQIAD----DEELQKYSKLLPIHTLRLGMEVDSFDGHHYISSVAPGGPVDTLNLLQPEDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRRL----FDD-----EASVDEPRTMEPALLEAE-------------VDHSVDVNIEDDDDGELALWS---------PEV---------------------KTVELVKDCKGLGFSILDYQDPLDPTRSVI--VIRSLVADGVAERSGELLPGDRLVSVNEFSLDNATLAEAVEVLKAVP 763 HSP 10 Score: 72.0182 bits (175), Expect = 5.432e-11 Identity = 143/601 (23.79%), Postives = 243/601 (40.43%), Query Frame = 0
Query: 1592 LNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEP----------------HKDNPIERYSGKYKGLHEVSILKGE-QGLGIMI---IEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGD-ESSNPATCEITPGADTVIEISK------DKDENGK--------PMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE--DD-----------------EIYETLDVELNKKKD-------------------RGLGLSIVGKK-----SGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMT-QGKIAMRVRRLRVGNRKQGVGGMHSR---DSSIPSN 2109
+N G+ GLG + G K V + + P G A RD R+Q GD IL++ G + V+ ++N +S V ++ R V ++ PP P +P E YS V ++K + Q LGI I + H SG++V + PGS A G +Q D I+AV+G + G + +L+N+ ++ + + + S SA +P ++ +T A+ PK+P + A +PKP A + A G + + K TV++ + KP P + G E+ + E G D + ++ D +E K + LG+ + D+ G +I V G L+P D++LEV L S A+ L++ P L+ R DD E+ ++DV + D +GLG SI+ + + + I +V G A+ G L+ GD++++VN L N++ +A +LK G + + + + V + K+ +HS DSS P++
Sbjct: 252 INDGS-GLGFGIVGGKSSG---VVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNS-VRMLVARD-------PVGEIAVTPPTPVSLPVALPAVATRTLDSDRSPFETYS--------VELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVAVDGVNIQGFANQDVVEVLRNAGQVVHLTLVR-----RKTSLSA--------SPFEHSSSRET-VAEPPKVPER-AGSPKPEA-------NLSVEAEEIGERLDNL---KNNTVQA----LEKPDVYPEKVPGSPENELKSRWENLLGPDYEVMVATLDAQIADDEELQKYSKLLPIHTLRLGMEV----DSFDGHHYISSVAPGGPVDTLNLLQPEDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRRLFDDEASVDEPRTMEPALLEAEVDHSVDVNIEDDDDGELALWSPEVKTVELVKDCKGLGFSILDYQDPLDPTRSVIVIRSLVADGVAERSGELLPGDRLVSVNEFSLDNATLAEAVEVLKAVPPGVVHLGICKPLVEDEKEERFSLHSNNNGDSSEPAD 799 HSP 11 Score: 70.0922 bits (170), Expect = 2.052e-10 Identity = 60/230 (26.09%), Postives = 108/230 (46.96%), Query Frame = 0
Query: 1589 YVSLNK-GTNGLGISLAGHKDRSK--MSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPL--SQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVA-NPIGPVKSDSPSAITAPVSPPA 1811
Y+ + + T GLG S+ + +S + +++ +HP A RD R++ D IL +N + +N+ +L ++ S+ + + + +Q + + + EV ++ GLG I+ GK SGV V + PG AD G LQ GD IL + G + G+ E A++L+N ++M+VA +P+G + P+ ++ PV+ PA
Sbjct: 134 YIDIERPSTGGLGFSVVALRSQSLGLIDIFVKEVHPGSVADRDHRLKENDQILAIND-----------TPLDQNISHQQAIALLQQATGSLRLVVAREVGHTQGRASTSSADTTLPETVCWGHTEEVELINDGSGLGFGIVGGK----SSGVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVARDPVGEIAVTPPTPVSLPVALPA 348 HSP 12 Score: 68.9366 bits (167), Expect = 4.760e-10 Identity = 105/422 (24.88%), Postives = 178/422 (42.18%), Query Frame = 0
Query: 149 IALSKPEGHSLGFSVVG-LKSEHKGEL-GIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSA-------EDSESTQSPPETTALPSRESTPRVLNSDWCQVEVI-ELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQI--------NDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDA----SQVKPHIPILPSSMLAKRAE-LETH-LQIAVVANSSFVSAASHGPPPLTNLANDMILPTQ--TEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCERE-ELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRST 543
+ L K +G SLG +VG + + H GE GIYV+ I P A +GQ+ D+I+A+DG + ++ + +L+ A VHL + R E S S ++ E +P R +P+ + + E I E +N + V +N + + L DY + + +D LQ + + LR + EVD D S V P P+ ++L E LE + +Q+ + VS PPP T + + + ++ +P+ L VD + E+ G A PE++T VELVKD +GLG +I Y + S I ++S+ A +SG ++ D+++ V+ S+ + +AVE+L++
Sbjct: 366 VELVKKDGQSLGIRIVGYVGTAHPGEASGIYVKSIIPGSAAYHNGQIQVNDKIVAVDGVNI-QGFANQDVVEVLRNAGQVVHLTLVRRKTSLSASPFEHSSSRETVAEPPKVPERAGSPKPEANLSVEAEEIGERLDNLKNNTVQALEKPDVYPEKVPGSPENELKSRWENLLGPDYEVMVATLDAQIADDEELQKYSKLLPIHTLR---------LGMEVDSFDGHHYISSVAPGGPVDTLNLLQPEDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRRLFDDEASVDEPRTMEPALLEAEVDHSVDVNIEDDDDGELALWS-------------PEVKT--VELVKDCKGLGFSILDYQDPLDPTRSVIVIRSLVADGVAERSGELLPGDRLVSVNEFSLDNATLAEAVEVLKAV 762 HSP 13 Score: 64.6994 bits (156), Expect = 1.116e-8 Identity = 35/98 (35.71%), Postives = 57/98 (58.16%), Query Frame = 0
Query: 1904 PGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHREDDEI 2001
PG +IE+ KDK+ GLGLS+ G D +IF+ + +G A DGR++ GD++LE+ ++ L SH +A ++ P RV+L+ R +D +
Sbjct: 1240 PGELHIIELEKDKN------GLGLSLAGNKDRSRMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPTRVKLVFIRNEDAV 1331 HSP 14 Score: 63.1586 bits (152), Expect = 3.248e-8 Identity = 60/239 (25.10%), Postives = 103/239 (43.10%), Query Frame = 0
Query: 145 DVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVV----------ARGSAEDSESTQSPPETTALPS---------------RESTP--RVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGV--VVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS 354
+V + L K G LG S+VG +S G+++ +I G A DG+LI GDQIL+++G+ + + S IL+ +G V L + +R ++++S+ Q ++ PS R S P + + VE+I ++ G G + + + NGVA + +L GD I+ IN L G N+L+ + + L V + + S
Sbjct: 1565 EVFLVDLQKKTGRGLGLSIVGKRSGS----GVFISDIVKGGAADLDGRLIRGDQILSVNGEDM-RHASQETVATILKCVQGLVQLEIGRLRAGSWAASRKTSQNSQGDQHSAHSSCRPSFAPVITSLQNLVGTKRSSDPPQKCTEEEPRTVEIIRELSDALGISIAGGKGSPLGDIPIFIAMIQANGVAARTQKLKVGDRIVSINGQPLDGLSHTDAVNLLKNAFGRIILQVVADTNIS 1798 HSP 15 Score: 61.2326 bits (147), Expect = 1.020e-7 Identity = 38/104 (36.54%), Postives = 59/104 (56.73%), Query Frame = 0
Query: 128 ENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVV 231
E EPR +I++ S +LG S+ G K G++ I++ IQ G+AA+ +L GD+I++I+GQPLD +SH A+N+L+ A GR+ L V
Sbjct: 1704 EEEPRTVEIIRELSD---------------ALGISIAGGKGSPLGDIPIFIAMIQANGVAARTQKLKVGDRIVSINGQPLD-GLSHTDAVNLLKNAFGRIILQV 1791 HSP 16 Score: 58.5362 bits (140), Expect = 7.805e-7 Identity = 29/86 (33.72%), Postives = 50/86 (58.14%), Query Frame = 0
Query: 468 TLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR 553
T +VEL+ D GLG I G SG+ V+++ G A + G + D I+++ G ++QG +++Q ++LR+ G SVR+ + R
Sbjct: 246 TEEVELINDGSGLGFGIVGGKS-----SGVVVRTIVPGGLADRDGRLQTGDHILKIGGTNVQGMTSEQVAQVLRNCGNSVRMLVAR 326
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|46577598|sp|Q24008.2|INAD_DROME (RecName: Full=Inactivation-no-after-potential D protein) HSP 1 Score: 196.052 bits (497), Expect = 1.180e-50 Identity = 140/446 (31.39%), Postives = 220/446 (49.33%), Query Frame = 0
Query: 1349 VRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDPVPTPVMTGSEDSATEIPGDDGQSS--SSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEG----EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIV 1788
V + + + GI IV G+V+ SP TGIFIK ++ SPA L L GDRIL+++G D+ +T +D+I+++ +E +Q+ + QA S S+ + + + + +S S +DED+ ED+ G+ G ID+ SA E E+EDEFGY+ KI K+Y K + LG++LAGHKDR KM+ ++ G+ P+G A ++ GD I+EVNG+V+ +R HLN S+ KN+ + I R + + + VKP+ +FP + I K + + ++ E LGIM+I GKHA+VGSG+F+SDL+ GS A++AG++ GDM+LAVN + + NY+ A +LK +EG++ MI+
Sbjct: 18 VTLDKTGKKSFGICIVRGEVK-------DSPNTK---TTGIFIKGIVPDSPAHLCGRLKVGDRILSLNGKDVRNSTEQAVIDLIKEADFKIELEIQT---FDKSDEQQAKSDPRSNGYMQAKNKFNQEQTTNNNASGGQGMGQGQGQGQGMAGMNRQQSMQKRNTTFTASMRQKHSNYADEDD-EDTRDMTGRIRTEAGYEIDRASAGNCKLNKQEKDRDKEQEDEFGYTMAKINKRYNMMKDLRRIEVQRDASKPLGLALAGHKDRQKMACFVAGVDPNG-ALGSVDIKPGDEIVEVNGNVLKNRCHLNASAVFKNVDGDKLVMITSRRKPNDEGMCVKPIKKFPTASDETKFIFDQFPKARTVQ----VRKEGFLGIMVIYGKHAEVGSGIFISDLREGSNAELAGVKVGDMLLAVNQDVTLESNYDDATGLLKRAEGVVTMIL 444 HSP 2 Score: 65.855 bits (159), Expect = 3.315e-9 Identity = 60/197 (30.46%), Postives = 91/197 (46.19%), Query Frame = 0
Query: 1897 PATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLL--GAIFIHEVYEKGAAHKDGRLKPGDQILEV----VHEDLRNVSHSHAL-HALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
PAT EI P +IE+ +K KPMG+ + GG + + G + H VY +G D RLK D I ++ +H H L H + V L + R D E +V+L KK + LGLS+ + G I+++++G + D +L +GD I NG L + + K GK++M V R
Sbjct: 477 PATAEIKPNKKILIELKVEK----KPMGV--IVCGGKNNHVTTGCVITH-VYPEGQVAADKRLKIFDHICDINGTPIHVGSMTTLKVHQLFHTTYEKA--VTLTVFRADPPELEKFNVDLMKKAGKELGLSL--SPNEIGCTIADLIQGQYPEIDSKLQRGDIITKFNGDALEGLPFQVCYALFKGANGKVSMEVTR 662
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|341940913|sp|Q91XL2.2|LNX2_MOUSE (RecName: Full=Ligand of Numb protein X 2; AltName: Full=Numb-binding protein 2) HSP 1 Score: 157.918 bits (398), Expect = 3.476e-38 Identity = 111/318 (34.91%), Postives = 169/318 (53.14%), Query Frame = 0
Query: 1905 GADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE----------------DDEIYETLDVELNKKKD-RGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR-----LRVGNRKQGVGG--------MHSRDSSIPSNVPGATGGTMKTVELKRGQH-GLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILK 2191
G T IEI + + LG+SIVGG++T L I I EVY G +DGRL GDQIL+V + D+ NVSH+HA L Q + ++L + RE DE+++ L L+K+ LG+ +V + PGVFI ++++GG A DGRL D+++ +NGHDL + + E AA I++ + ++ + + R G+R+ G HSR S ++ K + +K+ H LG ++ GG GS G++PI+V +V G A G +KRGD +L++N L LSH +AV +LK
Sbjct: 228 GEITTIEIHRSNPY----IQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYDISNVSHNHARAVLSQPCSTLQLTVLRERRFGSRANSHADGSAPRDEVFQVL---LHKRDSTEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLNSNDRVLAINGHDLKHGTPELAAQIIQASGERVNLTIARPGKPQPSNGSREAGAHSSSNHAQPPSHSRPGS-HKDLTRCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK 537 HSP 2 Score: 92.4337 bits (228), Expect = 1.796e-17 Identity = 88/335 (26.27%), Postives = 143/335 (42.69%), Query Frame = 0
Query: 1925 LGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI----------------------------------HREDDEIYETLDVELNKKKD--RGLGLSIVG---KKSGP-GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSN---------------VPGATGGTMKTVELKRGQHG-LGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
LG+ +V +D +FI ++ E G A +DGRL D++L + DL++ + A ++ + RV L I H++ + + KK+ LG+++ G KSG +F++ V G DGR+ +GD ++ +NG DLTN S +A +LK + A+ ++ L V ++ + S+ N +P A V L+R G GFSIVGG+ H + P ++KT+ A G LK GD I++VN G+SH V +LK V L ++
Sbjct: 350 LGIKLVRRTDE--PGVFILDLLEGGLAAQDGRLNSNDRVLAINGHDLKHGTPELAAQIIQASGERVNLTIARPGKPQPSNGSREAGAHSSSNHAQPPSHSRPGSHKDLTRCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLKASAASPAVILKALEVQIAEEAAQATEEQPSAFSENEYDASWSPSWVMWLGLPSALHSCHDIV-LRRSYLGSWGFSIVGGYEENHTNQPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVIC 681 HSP 3 Score: 78.1814 bits (191), Expect = 4.725e-13 Identity = 74/259 (28.57%), Postives = 116/259 (44.79%), Query Frame = 0
Query: 119 LETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGH-------------SLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR----GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR--EVDPSDASQ 358
L A P EP + S D LS PEG LG S+VG + I +QE+ G+ A+DG+L+ GDQIL ++ + SN+SH+ A +L + + L V R GS +S + S P R+ +VL + ++ G ++ GV + +++ G+A QDGRL++ D +L IN H L+ E A +++ SG V L +AR + PS+ S+
Sbjct: 191 LTPASLPVWTEEPGLDNPAFEESAAADSVQQPLSLPEGEITTIEIHRSNPYIQLGISIVG--GNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYDI-SNVSHNHARAVLSQPCSTLQLTVLRERRFGSRANSHADGSAP-------RDEVFQVL---------LHKRDSTEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLNSNDRVLAINGHDLKHGTPELAAQIIQASGERVNLTIARPGKPQPSNGSR 430 HSP 4 Score: 71.2478 bits (173), Expect = 7.196e-11 Identity = 61/224 (27.23%), Postives = 102/224 (45.54%), Query Frame = 0
Query: 147 KHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVV-------------------ARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
KHI + K SLG +V G + GEL I+V + P G A+DG++ GD +L I+G L +N+SH +A+ +L+ + +++ + S + +++ SP L P L+S C V+ + G+ +GF ++ G + +KT+V A DGRL GD I+ +N G + +L++ N V L V
Sbjct: 464 KHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDL-TNLSHSEAVAMLKASAASPAVILKALEVQIAEEAAQATEEQPSAFSENEYDASWSPSWVMWL----GLPSALHS--CHDIVLRRSYLGS-WGFSIVGGYEENHTNQPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTV 679
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|29840784|sp|Q8N448.1|LNX2_HUMAN (RecName: Full=Ligand of Numb protein X 2; AltName: Full=Numb-binding protein 2; AltName: Full=PDZ domain-containing RING finger protein 1) HSP 1 Score: 154.451 bits (389), Expect = 5.748e-37 Identity = 112/321 (34.89%), Postives = 170/321 (52.96%), Query Frame = 0
Query: 1905 GADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE----------------DDEIYETLDVELNKKKDRG--LGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR---LRVGNRKQGVGG------------MHSRDSSIPSNVPGATGGTMKTVELKRGQH-GLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILK 2191
G T IEI + + LG+SIVGG++T L I I EVY G +DGRL GDQIL+V + ++ NVSH++A L Q N + L + RE +EI++ V L+ K+D G LG+ +V + PGVFI ++++GG A DGRL D+++ +NGHDL + E AA I++ + ++ + + R + GN + G +SR SS ++ K + +K+ H LG ++ GG GS G++PI+V +V G A G +KRGD +L++N L LSH +AV +LK
Sbjct: 229 GEITTIEIHRSNPY----IQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNISNVSHNYARAVLSQPCNTLHLTVLRERRFGNRAHNHSDSNSPREEIFQ---VALH-KRDSGEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTPPPYYSRPSS-HKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK 540 HSP 2 Score: 97.4413 bits (241), Expect = 4.902e-19 Identity = 97/399 (24.31%), Postives = 168/399 (42.11%), Query Frame = 0
Query: 1862 SGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMI------------------------------------HREDDEIYETLDVELNKKKD--RGLGLSIVG---KKSGP-GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGG--------------TMKTVELKRGQHG-LGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
S V + A V S N R+ G+ + N + + + + +++ K ++G+ LG+ +V +D +FI ++ E G A +DGRL D++L + DL+ + A ++ + RV L I H++ + + + KK+ LG+++ G KSG +F++ V G DGR+ +GD ++ +NG DLTN S +A +LK + A+ ++ L V ++ + S+ N A+ + + L+R G GFSIVGG+ H + P ++KT+ A G LK GD I++VN G+SH V +LK V L ++
Sbjct: 292 SNVSHNYARAVLSQPCNTLHLTVLRERRFGNRAHNHS--DSNSPREEIFQVALHKRDSGEQ--LGIKLVRRTDE--PGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTPPPYYSRPSSHKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLKASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHSCHDIVLRRSYLGSWGFSIVGGYEENHTNQPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVIC 684 HSP 3 Score: 83.1889 bits (204), Expect = 1.609e-14 Identity = 75/257 (29.18%), Postives = 113/257 (43.97%), Query Frame = 0
Query: 125 PRLENEPRFQALIQQASQGRDVKHIALSKPEGH-------------SLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-----GSAEDSESTQSPPE---TTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVK 360
P L+N P F+ S G D LS PEG LG S+VG + I +QE+ G+ A+DG+L+ GDQIL ++ + SN+SH+ A +L + +HL V R A + + SP E AL R+S G G ++ GV + +++ G+A QDGRL + D +L IN H L+ E A +++ SG V L +AR P + ++
Sbjct: 204 PGLDN-PAFEE-----SAGADTTQQPLSLPEGEITTIEIHRSNPYIQLGISIVG--GNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNI-SNVSHNYARAVLSQPCNTLHLTVLRERRFGNRAHNHSDSNSPREEIFQVALHKRDS--------------------GEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIR 431 HSP 4 Score: 73.9442 bits (180), Expect = 8.938e-12 Identity = 68/220 (30.91%), Postives = 104/220 (47.27%), Query Frame = 0
Query: 147 KHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARG---------RVHLV-VARGSAEDSESTQSPPETTALPSREST-----PRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
KHI + K SLG +V G + GEL I+V + P G A+DG++ GD +L I+G L +N+SH +A+ +L+ + V +V A +AE+ ST S E A S P L+S C V+ + G+ +GF ++ G + +KT+V A DGRL GD I+ +N G + +L++ N V L V
Sbjct: 467 KHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDL-TNLSHSEAVAMLKASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHS--CHDIVLRRSYLGS-WGFSIVGGYEENHTNQPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTV 682
BLAST of conserved hypothetical protein vs. SwissProt
Match: gi|29840781|sp|O70263.1|LNX1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase LNX; AltName: Full=Ligand of Numb protein X 1; AltName: Full=Ligand of Numb-binding protein 1; AltName: Full=Numb-binding protein 1; AltName: Full=RING-type E3 ubiquitin transferase LNX) HSP 1 Score: 142.124 bits (357), Expect = 6.284e-33 Identity = 114/352 (32.39%), Postives = 173/352 (49.15%), Query Frame = 0
Query: 1883 VASPRRKAGDESSNPATCEITPGADTVI---EISKDKDENGKPM-GLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHREDD----------EIY----ETLDVELNKKK-DRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRV-RRLR------------VGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQIL 2202
VA+ + D S N E+ P +I EI+ K P L + +VGGS+T L I I +Y G +DGRL PGD IL+V D+ NV H++A+ LRQ +RL + RE + Y ++ V LNK + LG+ +V + PGVFI V+ GG AD G+L + D+++ +NGHDL S E AA +++ ++ ++ + V R++R + N +Q G +S P+ AT K LG ++ GG D+PIYV +V G + G +K GD +L+VN L +S +AV ILK+ +V+L+ L
Sbjct: 244 VANHVDQGRDNSENTTVPEVFPRLFHLIPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAHVPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSPGPGERNTASKPA----ATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSAPSSVVLKAL 591 HSP 2 Score: 90.5077 bits (223), Expect = 7.425e-17 Identity = 81/329 (24.62%), Postives = 140/329 (42.55%), Query Frame = 0
Query: 1925 LGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE----DDEIYETLD----------------------------VELNKKKDRGLGLSIVG----KKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATG-------------GTMKTVELKRGQHG-LGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
LG+ +V D +FI V G A + G+L+ D++L + DLR S A H ++ + RV L++ R+ +I++ V + K LG+++ G ++ +++ V GG DGR+ GD ++ VNG +LT S+ +A ILK + ++ ++ ++ + DS+ PG K V L+R G LGF IVGG+ G+ P ++K++ + A + G ++ GD +L+VN + G+ H +LK G + L I S
Sbjct: 397 LGIKLVRRVDE--PGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSPGPGERNTASKPAATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSAPSSVVLKALEVKEQEAQEDCSPA-ALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTAGSLGFCIVGGYEEYSGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIAS 722 HSP 3 Score: 73.1738 bits (178), Expect = 1.827e-11 Identity = 52/176 (29.55%), Postives = 88/176 (50.00%), Query Frame = 0
Query: 176 IYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREV 351
I +Q I G+ A+DG+L+ GD IL ++G + SN+ H+ A+ +L++ + L V R S S P++ P +S +LN ++ G ++ GV + V+ GVAD+ G+L D +L IN H L+ E A++++ S V L+V+R+V
Sbjct: 305 IIIQHIYRDGVIARDGRLLPGDIILKVNGMDI-SNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAHVPDSYG-PRDDSFHVILNK----------SSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQV 468 HSP 4 Score: 71.2478 bits (173), Expect = 7.767e-11 Identity = 58/219 (26.48%), Postives = 103/219 (47.03%), Query Frame = 0
Query: 147 KHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSREST--PRVLNSDW----------CQVEVIELTNNGAG-FGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVA 348
K +++ K SLG +V G S + +L IYV ++P G+ ++DG++ GD +L ++G L + +S +A+ IL+ A V L ++++ SP AL S + P + W C + + L N AG GF ++ G + +K++V+ A DGR+ GD +L +N G +A +L++ + L +A
Sbjct: 507 KVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIEL-TEVSRTEAVAILKSAPSSVVLKALEVKEQEAQEDCSP---AALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTAGSLGFCIVGGYEEYSGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIA 721 HSP 5 Score: 59.3066 bits (142), Expect = 3.228e-7 Identity = 69/264 (26.14%), Postives = 109/264 (41.29%), Query Frame = 0
Query: 78 ILPSDFDIGVNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVK-HIALSK--PEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-------------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNN-----GAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQIN 320
+LP D + VNG I N+P A+ R P L + + R A + + RD H+ L+K PE LG +V E G+++ + G+A + GQL E D++LAI+G L S A +++Q + RVHLVV+R G + + + P E +T C +V+ + + G G G + + V +V GV +DGR+ GD +L +N
Sbjct: 322 LLPGDIILKVNGMDISNVPHNYAVR--------LLRQPCQVLRLTVLREQKFRSRSNAHVPDSYGPRDDSFHVILNKSSPE-EQLGIKLV----RRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFG-SPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSPGPGERNTASKPAAT--------CHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVN 563
BLAST of conserved hypothetical protein vs. nr
Match: gi|1325330388|ref|XP_023347062.1| (multiple PDZ domain protein-like [Eurytemora affinis] >gi|1325330390|ref|XP_023347063.1| multiple PDZ domain protein-like [Eurytemora affinis]) HSP 1 Score: 1390.94 bits (3599), Expect = 0.000e+0 Identity = 890/1740 (51.15%), Postives = 1094/1740 (62.87%), Query Frame = 0
Query: 539 MLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSE---------SSESLQDLENQPEIFSETSE---EYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCAR--SQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLP--EVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTE------RMSPSVNLPDLPQDDDTPIPPPLPT---SPP--PEDGDFDARTAERLLGTTSKGVISARFV-----------SREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSASTTASREERILSSVERQEPQAS-VSEKANGTTVISIRNSAEPQQESGALDTCYTSASSTKSNAQI-----SAPLVSPEA--SDIASVFGGVQSPERTLTIQSVTLDESSTDYPSGTEKDPSQFPVVPPRPSKAASASSSSASASSSSTSSPAKIVSTRDFSPSGAGSGRVGGGASSVSSGGISGGEAPDEDDRDHSTTITSTTASNVFSSQNWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQA-DSPTHSDMSESVATSL----------PPPPPPPPLI---DSVPPG-FAND---------PVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGG---GGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHREDDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
MLR+TG VRL+L+RYVHGLKFEQLQQAIA+S + + P+ + + RS + DS LSE +++ ++ E+ +E ++ + W +G I++A I K K GGLGISLEGTVEKVDG EQNPHHYIRSVLPNGPVG NG L SGDELLEVNGRKLLGLYH DVV ILK+LPM V+LVCAR S+ + + +ER+VKAKSD S+SS+ T S+LKS+SLEPLT LAMWSD +L I L K ERGLGFSILDYQDP+N ETVIVIRSLVPGGV+QQDGRLIPGDRLM+VND LE+A+LD AVQALKGAP+G VRIGV KPLP+P ++ E E+D+ DNTEV S SD+ T+ R S S +PDLP T P T S P E ERL+G TSK V +F +R E + D +PPLPAALE+ I + KD DTLG+QVDI+D G+NG+VVRS+T GGTL++DGRI PGDYLV+VN E MR S +QAL +LRRT ++P ++ ITYIPA DA V+R + +TR+AE EE G S + S E ++ + S VSE++NGTTVISI + E + + + K Q+ APLVSPE S SV G + +R ++++ VT+ ESSTD P EK P P PR S ++S R SP S G S +S +D+ S+ S++WGPER + IQRVPNQGLGISIVGGK+E AP+ G + +TGIFIKNVL+GSPA + L TGDRI+AV D+ A+HD+AV+VIRQSG + VVQSL T S ++ D+ D E A ++ PP L+ D PP FAN+ PV +P S +PG G + S SD D GQ TLSNG+ ID++SA FL + ++ E+ED++GY+ +KI +KY + G + LNKGT GLGISLAGHKDR KM+V + GL+P GNA+RD +M+VGD+I+EVNG V+ R HLN SS IKNLPD+ VTF++LR D ++NL KP+ QFP ++NPI+RY KYKGL +V++ KGE GLGIMIIEGKH + G+GVF+SDLQ GS AD AGL GDMIL+VNGEDFVG +YE+AA++LK ++G IK+IVANP +P T + ES +KPKLPPKP +APKP + A GG GG++ G KAT P + D + TCEI PG DTVIEI KDKDE GKPMGLGLSIVGGSDTLLGAIFIHEVYE GAA KD RL+PGDQILEV+ EDLRNV+HS ALHALRQTPN+VRL+IHREDDEIYE+L+VEL KK++RGLGLSIVGKKSGPGVFISEVVKGGAAD DGRLVQGDQII+VNG DL N+SQE+AAP+LKM QGKI M VRRL+VGNR++ +GG D S+P NV T GT KT+ L RGQ+ LGFSIVGGFGSPHGDMPI+VKTVF+ GAAA+HG LKRGDQILSVN L+GL+H AV ILKNC+G V LQILS
Sbjct: 1 MLRATGAVVRLKLIRYVHGLKFEQLQQAIASSAAPTPTANPASFGLDL------RSPVSDKSPKHKPPPPKRTDSLLSEDITPPKEVLEAQAKAGVDYAGELSAEVTDAIMKKWSKIVGPEFEIVVAQISKYKEGGGLGISLEGTVEKVDGAEQNPHHYIRSVLPNGPVGQNGNLVSGDELLEVNGRKLLGLYHADVVTILKELPMDVRLVCARPCSKVKEDKPSPSPVPTPSPAPVSGLTPVSERLVKAKSDGSLSSSAT-----DSAVLSRLKSKSLEPLTGLAMWSDDILTISLQKTERGLGFSILDYQDPLNPHETVIVIRSLVPGGVAQQDGRLIPGDRLMFVNDTPLENANLDTAVQALKGAPQGEVRIGVAKPLPVPDSQIGEGPTEEDTT---------DNTEVQSAISDIETDPGEKDRRGSISSEIPDLPPPLPTSPLPSEDTENQSSPVRAEQKGIALPATERLIGGTSK-VFETKFEERSSKGSLMVETRYEEKTDADNIPPLPAALEQRIKIIKDGDTLGLQVDIEDGGVNGMVVRSLTRGGTLAKDGRIQPGDYLVSVNNENMRGVSQNQALAVLRRTQLVPQGGQVPITYIPAQDAVVFRTTMLTRVAE-------EETAGHSRRSRSISVERSLIPGTDLTTATVSAVSERSNGTTVISISSHIE----TADMKPTPPVSPEMKPTPQVLSEMKPAPLVSPEIEFSSAESVAG--KGLDR-ISLKYVTVSESSTD-PEPVEKRPLSPPEAAPRTS----------------------LLSERMNSPDLPKSRTPSLGRSKLSQS--------SKDEYFPSS-----------PSKHWGPERTIIIQRVPNQGLGISIVGGKIE--APE-----GSDSSALTGIFIKNVLDGSPAAATGQLNTGDRIIAVGDVDIRHASHDKAVEVIRQSGNPLHLVVQSLNLWTEDSFNESQDTINEQDDIEKEAETILNDLDRTIGGEENVIPPSLLLTEDFSPPEEFANESFSQTLSDPPVASP------RSELVVPGI-GPIRRTESTDSDSSTDSDEAEQQGQETLSNGITIDRSSAGFLGRAINDSEDEDDYGYTMKKINRKYGK-HGDLHCIRLNKGTEGLGISLAGHKDRLKMAVIVAGLNPKGNAYRDGKMKVGDIIMEVNGIVLLHRSHLNASSVIKNLPDAGVTFVVLRKDDPLENLGAKPVIQFPTT-LEENPIDRYR-KYKGLRQVTLKKGETGLGIMIIEGKHPEAGTGVFISDLQDGSVADSAGLLVGDMILSVNGEDFVGASYETAAKVLKKTDGEIKIIVANP----------------------NLPET-RVESPEKPKLPPKPVLAPKPVNLGA---------AKGGERDGGRSPG--GGKAT-------------PPPAKDKIDHT----TCEIVPGHDTVIEIIKDKDEEGKPMGLGLSIVGGSDTLLGAIFIHEVYEAGAAFKDRRLRPGDQILEVMKEDLRNVTHSFALHALRQTPNKVRLVIHREDDEIYESLEVELIKKRERGLGLSIVGKKSGPGVFISEVVKGGAADVDGRLVQGDQIISVNGQDLKNASQEEAAPVLKMAQGKIIMCVRRLKVGNREKRIGG----DQSLPPNV--VTTGTPKTISLIRGQNNLGFSIVGGFGSPHGDMPIFVKTVFEKGAAAEHGELKRGDQILSVNGTELQGLTHQHAVEILKNCEGTVTLQILS 1589 HSP 2 Score: 84.3445 bits (207), Expect = 3.218e-12 Identity = 124/554 (22.38%), Postives = 225/554 (40.61%), Query Frame = 0
Query: 59 LNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQALASPVHSDPPAPRSPPSPLETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVA---------------------------------RGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQV-----EVIELTNNG------AGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPI----QEELLMGTTARSKM--HSSLMGVSQGDMPEMETL----DVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
+ + D + E+ V LH S L + +I NLPD + D P P+ LE P I + + + ++ + L K E LG ++ K G G+++ ++Q +A G L+ GD IL+++G+ S+ A +L+K G + ++VA +G D + + T P+++ ++ C++ VIE+ + G G ++ G + + V + G A +D RL GD IL++ L+ + LR++ N VRL++ RE D S L ++ KR E L +++V S F+S G ++ ++ Q +Q+ L + + +P+ Q +++M R K+ +G Q P + T + L++ LG +I G IFVK+V + AA + G + DQI+ V+G +QG ++Q AVE+L++ +V L+++
Sbjct: 1073 MKVGDIIMEVNGIVLLHRSHLNAS-------SVIKNLPDAGVTFVVLRKDDPLENLGAKPV-IQFPTTLEENP-----IDRYRKYKGLRQVTLKKGE-TGLGIMIIEGKHPEAGT-GVFISDLQDGSVADSAGLLV-GDMILSVNGEDF-VGASYETAAKVLKKTDGEIKIIVANPNLPETRVESPEKPKLPPKPVLAPKPVNLGAAKGGERDGGRSPGGGKATPPPAKDK----IDHTTCEIVPGHDTVIEIIKDKDEEGKPMGLGLSIVGGSDTLLGAIFIHEVYEAGAAFKDRRLRPGDQILEVMKEDLRNVTHSFALHALRQTPNKVRLVIHREDDEIYES--------LEVELIKKR---ERGLGLSIVGKKSGPGVFISEVVKGGA--ADVDGRLV---QGDQIISVNGQDLKNASQEEAAPVLKMAQGKIIM-CVRRLKVGNREKRIGGDQSLPPNVVTTGTPKTISLIRGQNNLGFSIVGGFGSPHGDMPIFVKTVFEKGAAAEHGELKRGDQILSVNGTELQGLTHQHAVEILKNCEGTVTLQIL 1588
BLAST of conserved hypothetical protein vs. nr
Match: gi|1233168959|ref|XP_022186552.1| (LOW QUALITY PROTEIN: multiple PDZ domain protein [Nilaparvata lugens]) HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0 Identity = 557/1226 (45.43%), Postives = 714/1226 (58.24%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQ----DLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLP-----------------DGQAL--ASPVHSDPPAPRSPP---SP------LETAMAPRLENEPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQS-------------------PPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLT-NLANDMILPTQTEQVSIY------------QPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARS--SPQG-----VDNEVDNKGESGG--DSELSESSESLQ---DLENQPEIFSETSE------------EYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVG----TKQDFP-------LGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQD--DDTPIPP-----PLPTSPPP-EDGDFDARTAERLLGTTSKGVISARFVSREEP----IHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRAST 1116
MP+ D AL LL+ ++ + S D+ L+ DL LIS LE+P+F++I+ IQDSL EL Q+ HPSILP DFDI V G+L+LNLP D Q + A S P SPP SP L P E FQ I+++SQGRDV + L KP+G SLGFSVVGLKSE KGELGI+VQEIQP GIA +DG+L EGDQILAIDGQPLD ISH QAI ILQKARG V LVVAR + +T P + S+ + VLN++W QVEVI+L N+G+G GFG++ G+ + GVVVKT++ GVAD+D RL +GD+ILQI + G G EQVA+VLR+SG+HVRL+VAR V+P+ D + H PI+P+ +LA EL+ HL +G P +T N AN I+ T E IY S L V + T P L + T M Q +PEM+ VEL KD+ GLGITIAGY CE+EELSGIFVKS+++GSAA + VND+I+EVDG+S+QG++N +AVE+LR+TG++V L L RY+ G KFEQLQQAIANS+ + TP+ + SAT L R +P G +D +V+ +GES DS + E+L DLE+ + + E W LG I++ + K GGLGISLEGTV+ DG+E PHHYIRS+L GPVG N L+SGDELLEVNG +LLG+ H +VV ILKDLPM V++VCAR + + G T QD LGG+ ++ I A ER+VKAKSD S++S + P SKLKSRSLEPLT LAMWS IEL+KGERGLGFSILDYQDPMN ETVIVIRSLVPGGV+Q DGRLIPGDRL+ VND L++ASLD+AVQALKGA +G+VRIGV KPLP+P+ E ++++ T D++ + S LP D DD IPP LPT+ PP ED + D + + L E+ + E + PLPA+LE I K SD LG+ +D D G NG+VV ++ G+LS DGR+ GD ++AVN E +R+ S +QA +LRR ++ L +I +TYIP Y ST
Sbjct: 1 MPLNADISTALQLLESIQLTVNQS-------DDPKLQAQTSADLNLLISALENPIFRSIVTIQDSLSELNVQLHQHPSILPVDFDINVAGELVLNLPPPIYYPTTPTPTTNTELDDQLVPVAKLSQSSSGEPNSPPVISSPGAEDVSLPPITTPTYALE--FQKAIEESSQGRDVLTVKLFKPDGSSLGFSVVGLKSEEKGELGIFVQEIQPTGIAGRDGRLHEGDQILAIDGQPLDCAISHQQAIAILQKARGLVELVVARHPTTTASTTPPPASAASASAATPPPPPRDRSPSALSDTSKAGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGR-STGVVVKTILPGGVADRDARLQSGDHILQIVEATRDGMGSEQVASVLRQSGSHVRLVVARPVEPTAPDYQALGSHAPIVPTKILADPDELDRHL-------------VQNGYPEVTYNPANGHIIDTY-ENPFIYSGHDAADRDRHGHDSSLVVDVVRPTLPNTPSLPLLTMD--------MIPPQTQLPEMDKFTVELRKDSHGLGITIAGYVCEKEELSGIFVKSISEGSAADVCKKIQVNDRIVEVDGQSLQGYTNHEAVEVLRATGQTVCLCLERYLRGPKFEQLQQAIANSEL--KPPTPN----SPSATSLPRFPLNPDGESTTEMDVDVEPEGESRTTMDSLVLHEHEALSRASDLEDVQLLIDDDFEGPLEPAVESAIQAKWSKILGPDVEIVVGQLCKFGEGGGLGISLEGTVDVEDGQEVRPHHYIRSILAEGPVGQNNKLRSGDELLEVNGHRLLGMNHIEVVTILKDLPMHVRMVCARRKGDAPGFGRLIDTSQDRAAFAARNLLGGSLQNLIPA-MERLVKAKSDGSLAST-ATATVTTDPSLSKLKSRSLEPLTGLAMWSSEPHIIELLKGERGLGFSILDYQDPMNASETVIVIRSLVPGGVAQVDGRLIPGDRLLSVNDTALDNASLDQAVQALKGAAKGVVRIGVAKPLPIPDSVSHSQEFVEGLSQATPTLDDHSMYSCSG-------------SLPSDIEDDEDIPPALXXXALPTTLPPIEDLNTDDQLSNDELQIEEASTPDTLLQEEEDMALDVVGEETLKTPLPASLEVNIKFNKGSDPLGVTLDCCDGGANGMVVTELSDAGSLSHDGRVVVGDLIIAVNNESLRRVSKAQARAVLRRANL--LTAEINVTYIPLKAVLEYSRST 1171 HSP 2 Score: 116.316 bits (290), Expect = 5.829e-22 Identity = 79/189 (41.80%), Postives = 108/189 (57.14%), Query Frame = 0
Query: 1313 SGGEAPDEDDRDHSTTITSTTASNVFSSQNWGPERCVRIQRVPNQGLGISIVGGKVE-PNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPP--PLIDSVPPGFANDPVPTP 1498
S G+ DE R T ++ +S++ ++WGPER V++ R PN LGISIVGGKV+ NA D S ++GIFIKNVL SPAG + L TGDRIL VDG DL A+H++AVDVIR +G V F+VQSL+ + ++ S + + + P PP P L+ + P + PTP
Sbjct: 1463 STGQEVDES-RMTVTHAATSQSSSILLDKHWGPERAVKVLREPNSSLGISIVGGKVDLYNAGPDSGS------AISGIFIKNVLPLSPAGRTGELKTGDRILEVDGIDLRHASHERAVDVIRAAGNPVHFLVQSLVQWSVDGDGES-SGMEDGLRKGSSRRRAPAPPSPHGELLKTSPYPIPHPRTPTP 1643
BLAST of conserved hypothetical protein vs. nr
Match: gi|939695148|ref|XP_014291667.1| (PREDICTED: multiple PDZ domain protein-like [Halyomorpha halys]) HSP 1 Score: 810.446 bits (2092), Expect = 0.000e+0 Identity = 510/1127 (45.25%), Postives = 683/1127 (60.60%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQ----DLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLP----------------DGQAL--ASPVHSDPPAPRSPP---SPLETAMAPRLENEP---RFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALP--SRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPP-LTNLANDMILPTQTE-QVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSL-MGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTS-VGTKQDFP-------LGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDIL 1087
MP+ D +AL +L+ + + S D+V L+ DL +IS LE+P+F+ I IQDSL EL Q+Q HPSILP DFD+ G L+LNLP D Q + A S P SPP L+ P + FQ I ++SQGRDV + L KPEG SLGFSVVGL+SE KGELGI++QEIQ GIA +DG L EGDQILAIDGQPLDSNISH QAI+ILQKARG V LVVAR + S P +A+ S+ + VLN++W QVEVI+L N+G+G GFG++ G+ + GVVVKT++ GVAD++GRL +GD+ILQI D L+G G EQVA VLR+SG+HVRL+VAR V+P+ D + PI+P+ +LA EL+ HL + + + P P + N + + E QV P HL VD ++ G T ++ M +PEME VEL KD QGLGITIAGY CE+EELSGIFVKS++ GSAA + VND+I+EVDG+S+QG++N AVE+LRSTG +VRL L RY+ G KFEQLQQAIANS+ + TPS + SA L R P VD G+S E +S + +P + + ++ W +G I++ ++K + GLGISLEGTV+ DG+E PHHYIRS+LP GPVG NG L+SGDELLEVNG +LLG+ H +VV ILK+LP+ V++VCAR +++ + T QD L G+ ++ +L TER+VKAKSD S++S T + +P SKL+SRSLEP+T LAMWS IEL+KGERGLGFSILDYQDPMN ETVIVIRSLVPGGV+Q DGRLIPGDRL+ VN + L++ASLD+AVQALKGAP+G+VRI V KPLP+P+ E ++ +E + T D++ + S+ S++ D+ + IP L + ED + L +SK V+ LP LE+ I + ++ +LGI++ +GING++++ + G ++++ I GD++ +N E +R S SQAL +L
Sbjct: 5 MPLNADISSALMVLEHIQSTVNQS-------DDVKLQSKTNDDLNLIISFLENPIFRGIATIQDSLSELSTQIQQHPSILPIDFDVTEAGKLVLNLPPPLFEPDFITSPNTEVDDQRVPVAKLSQSSSGEPNSPPIINESLDDISLPPITTPTYALEFQKAIDESSQGRDVLTVKLFKPEGCSLGFSVVGLRSEEKGELGIFIQEIQSNGIAGRDGHLHEGDQILAIDGQPLDSNISHQQAISILQKARGLVELVVARSEDQQPSSLTPSPSPSAVSDSSKAGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGR-STGVVVKTILHGGVADRNGRLQSGDHILQIGDVNLRGMGSEQVAAVLRQSGSHVRLVVARPVEPTSPDYQALGSQAPIVPTKILADPEELDRHL-----VQNGYPQVTYNTPTPNVEPFDNPFMFHQEGELQVREQDPRHL---VD---------VIPGLTPPLLPLLTMDMLPLDTQLPEMERFTVELKKDIQGLGITIAGYVCEKEELSGIFVKSISVGSAADLCKKIQVNDRIVEVDGQSLQGYTNHDAVEVLRSTGITVRLSLERYLRGPKFEQLQQAIANSEL--KPPTPS----SPSAVSLPRF-PISVD----------GESTEIEVEDSEYEGPLKPAVEAAIKKK-WSEIMGPDVEIVVGQLKKFGESKGLGISLEGTVDVEDGQEVRPHHYIRSILPEGPVGQNGKLRSGDELLEVNGHRLLGMNHIEVVTILKELPVAVRMVCARRMGEASRLIDTSQDRAAFAARSILSGSLQN-LLPATERLVKAKSDGSLAST---TTAPTEPNLSKLRSRSLEPITGLAMWSSEPHIIELVKGERGLGFSILDYQDPMNPSETVIVIRSLVPGGVAQLDGRLIPGDRLLSVNSVALDNASLDQAVQALKGAPKGIVRIAVAKPLPIPDSVSNSQETETGISEETPTIDDHSMYSCSE--------SIHSEDIKKS----IPEDLDINALLEDQPGETEEKNENLEGSSKNVL-------------------LPVVLEKEIRIPTNNQSLGIELGSVADGINGMIIKEILPNGIIAQNKEISAGDFITRINNEALRNISESQALSLL 1053 HSP 2 Score: 326.635 bits (836), Expect = 4.295e-86 Identity = 213/528 (40.34%), Postives = 302/528 (57.20%), Query Frame = 0
Query: 1320 EDDRDHSTTITSTTASNVFSSQNWGPERCVRIQRVPNQGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVV----QSLLGVTAASSTQADSPTHSDMSESVATSLPPPPPPPPLIDSVPPGFANDPVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSDEDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHE----GEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDAR--MQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGE-QGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSP--PAPVVVPTTPQTES-----ADKPKL 1829
E + + T+TS+ ++ +FS ++WGP+RCV+I R P LGISIVGGKV+ K SS ++GIFIKN+L SPAG + L TGDRIL VDG DL A+H++AV+VIR + V F+V Q + G +AS + D ++E++ P + + + V ++P D SS S D + E G T + G+ I + SA + + E E+ED+FGY+ +K+ KKY R I L +G GLGISLAGHKDR+KM+V ICGL+P +R + +QVGD I+EVNG +++ R HLN S IK LP + T I +R ++++L+VKP++QFP ++ P ++YS +KGL VSI K QGLGIMIIEGKH++VG G+F+SD+Q GS A AGL GDMILAVN + VG NYE+AA +LK +EG++ ++V NP + D SP A ++ +TP+ + A PKL
Sbjct: 1284 ESSKQRTMTVTSSGSAILFS-KHWGPQRCVKIFREPKCSLGISIVGGKVDLYTSKPDSSSA-----ISGIFIKNILPNSPAGKTGELKTGDRILEVDGIDLRQASHERAVEVIRAANNPVTFLVQSLVQQVPGGESASLSDGDGKV---LTENLTERNPDKTIGKKTMQENESNVTSTTLQPEVQLTITSPVEKVPPDSPSSSEDSEEEEDYRQLE------GLTRSAAGIEISRASAGNVKRSKEEIEADPEKEDQFGYTAQKVKKKYGR---MAIVAELERGPQGLGISLAGHKDRTKMAVLICGLNPSTVVYRSTQGTLQVGDEIVEVNGVIVHGRCHLNASVIIKGLPGPNFTIIAIRKSDALEDLSVKPITQFPVSLQEETPEQKYSS-FKGLKTVSIKKHSGQGLGIMIIEGKHSEVGQGIFISDIQEGSAAHQAGLNVGDMILAVNKDTLVGSNYETAASLLKKTEGVVTLVVCNPNRAREEDKKHGDITLASPCTSASGILASTPKNDKGRHSQAGTPKL 1792 HSP 3 Score: 216.853 bits (551), Expect = 1.551e-52 Identity = 110/207 (53.14%), Postives = 149/207 (71.98%), Query Frame = 0
Query: 1892 DESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRED----DEIYETLD---VELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGN 2091
+E + AT I PG ++ IEI+K+K MGLGL+IVGGSDT +GA+ ++E+Y GAA KDGRLKPGDQILE+ +ED RN++++ AL LRQTP++VR++I+RE+ E LD VEL KK +GLGLSIVG+K G G+F+S++V GG A +DGRL++GDQI+ VNG D+ N S E AA +LK T GKI +R+ R + N
Sbjct: 1810 EEDNFAATAPIIPGEESTIEINKEK------MGLGLNIVGGSDTPMGAVVVNEIYPDGAAFKDGRLKPGDQILEINNEDFRNITNNKALAVLRQTPSKVRMVIYREEVPEGKEGIANLDIIEVELTKKSGKGLGLSIVGRKVGAGIFVSDIVPGGVASTDGRLMKGDQILAVNGQDIRNMSHEGAATVLKTTSGKICLRIGRFKASN 2010 HSP 4 Score: 102.449 bits (254), Expect = 9.797e-18 Identity = 71/201 (35.32%), Postives = 101/201 (50.25%), Query Frame = 0
Query: 2007 VELNKKKDRGLGLSIVGKKSGP--GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVEL-KRGQHGLGFSIVG---GFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
+E+NK+K GLGL+IVG P V ++E+ GAA DGRL GDQI+ +N D N + A +L+ T K+ M + R V K+G+ + + VEL K+ GLG SIVG G G I+V + G A+ G L +GDQIL+VN + + +SH A +LK G + L+I
Sbjct: 1828 IEINKEK-MGLGLNIVGGSDTPMGAVVVNEIYPDGAAFKDGRLKPGDQILEINNEDFRNITNNKALAVLRQTPSKVRMVIYREEVPEGKEGIANLD-----------------IIEVELTKKSGKGLGLSIVGRKVGAG-------IFVSDIVPGGVASTDGRLMKGDQILAVNGQDIRNMSHEGAATVLKTTSGKICLRI 2003 HSP 5 Score: 87.8113 bits (216), Expect = 2.982e-13 Identity = 62/198 (31.31%), Postives = 99/198 (50.00%), Query Frame = 0
Query: 1590 VSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKM 1786
+ +NK GLG+++ G D +V + ++P G AF+D R++ GD ILE+N + + + ++ P S V ++ R + + L E + + SGK GLG+ I+ K VG+G+FVSD+ PG A G L +GD ILAVNG+D +++E AA +LK + G I +
Sbjct: 1828 IEINKEKMGLGLNIVGGSDTPMGAVVVNEIYPDGAAFKDGRLKPGDQILEINNEDFRNITNNKALAVLRQTP-SKVRMVIYREEVPEGKEGIANLDIIEVE------LTKKSGK--------------GLGLSIVGRK---VGAGIFVSDIVPGGVASTDGRLMKGDQILAVNGQDIRNMSHEGAATVLKTTSGKICL 2001 HSP 6 Score: 87.4261 bits (215), Expect = 4.140e-13 Identity = 97/360 (26.94%), Postives = 153/360 (42.50%), Query Frame = 0
Query: 1922 PMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHK--DGRLKPGDQILEVVHEDLRNVSHSHALHALRQT--PNRVRLMIHRED----------------------DEIYETLD----VELNKKKDRGLGLSIV-GKKS--GPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRV---RRLRVGNRKQGVGGMHSRDS-------SIPSNVPG--ATGGTMK-----------------------------------TVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
P GLG+S+ G D A+ I + ++ G L+ GD+I+EV + H +A ++ PN + I + D ++ Y + V + K +GLG+ I+ GK S G G+FIS++ +G AA G L GD I+ VN L S+ E AA +LK T+G + + V R R ++K G + S + S P N G + GT K T+E+ + + GLG +IVGG +P G + V ++ GAA G LK GDQIL +N + ++++ A+ +L+ V + I
Sbjct: 1550 PQGLGISLAGHKDRTKMAVLICGLNPSTVVYRSTQGTLQVGDEIVEVNGVIVHGRCHLNASVIIKGLPGPNFTIIAIRKSDALEDLSVKPITQFPVSLQEETPEQKYSSFKGLKTVSIKKHSGQGLGIMIIEGKHSEVGQGIFISDIQEGSAAHQAG-LNVGDMILAVNKDTLVGSNYETAASLLKKTEGVVTLVVCNPNRAREEDKKHGDITLASPCTSASGILASTPKNDKGRHSQAGTPKLVPPPGSLSPSPSGKLKVEEDNFAATAPIIPGEESTIEINKEKMGLGLNIVGGSDTPMG--AVVVNEIYPDGAAFKDGRLKPGDQILEINNEDFRNITNNKALAVLRQTPSKVRMVI 1906 HSP 7 Score: 82.8037 bits (203), Expect = 9.072e-12 Identity = 60/195 (30.77%), Postives = 97/195 (49.74%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG ++VG G + V EI P G A +DG+L GDQIL I+ + NI++++A+ +L++ +V +V+ R + PE +E + N D +VE+ + +G G G ++ + AG+ V +V GVA DGRL GD IL +N ++ E A VL+ + + L + R
Sbjct: 1833 EKMGLGLNIVGGSDTPMG--AVVVNEIYPDGAAFKDGRLKPGDQILEINNEDF-RNITNNKALAVLRQTPSKVRMVIYR---------EEVPE-----GKEG---IANLDIIEVELTK--KSGKGLGLSIVGRKVGAGIFVSDIVPGGVASTDGRLMKGDQILAVNGQDIRNMSHEGAATVLKTTSGKICLRIGR 2005 HSP 8 Score: 73.9442 bits (180), Expect = 4.279e-9 Identity = 61/199 (30.65%), Postives = 92/199 (46.23%), Query Frame = 0
Query: 1921 KPMG--LGLSIVGGSDTLLG--AIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHREDDEIYETL-----------------DVELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG G IFI E+ G A +DG L GDQIL + + L N+SH A+ L++ V L++ R +D+ +L D+ LN + + GLG I+G +S GV + ++ GG AD +GRL GD I+ + +L E A +L+ + + + V R
Sbjct: 175 KPEGCSLGFSVVGLRSEEKGELGIFIQEIQSNGIAGRDGHLHEGDQILAIDGQPLDSNISHQQAISILQKARGLVELVVARSEDQQPSSLTPSPSPSAVSDSSKAGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGRS-TGVVVKTILHGGVADRNGRLQSGDHILQIGDVNLRGMGSEQVAAVLRQSGSHVRLVVAR 372 HSP 9 Score: 72.0182 bits (175), Expect = 1.880e-8 Identity = 61/209 (29.19%), Postives = 96/209 (45.93%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRR---LRVGNRKQGVGGMHSRDSSIPSN--VPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
+ L V+L K + LG S+VG +S G+FI E+ G A DG L +GDQI+ ++G L +N S + A IL+ +G + + V R + + DSS + V ++ ++L GLGF I+GG + + VKT+ G A +G L+ GD IL + L G+ +L+ V L +
Sbjct: 167 DVLTVKLFKPEGCSLGFSVVGLRSEEKGELGIFIQEIQSNGIAGRDGHLHEGDQILAIDGQPLDSNISHQQAISILQKARGLVELVVARSEDQQPSSLTPSPSPSAVSDSSKAGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGRST-----GVVVKTILHGGVADRNGRLQSGDHILQIGDVNLRGMGSEQVAAVLRQSGSHVRLVV 370
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842699|ref|XP_022239968.1| (multiple PDZ domain protein-like isoform X4 [Limulus polyphemus]) HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0 Identity = 534/1221 (43.73%), Postives = 705/1221 (57.74%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDS----ESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRL 1120
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA +DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S E T + S + + VLN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL 1168 HSP 2 Score: 588.956 bits (1517), Expect = 3.440e-170 Identity = 332/697 (47.63%), Postives = 455/697 (65.28%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS----DSPSAITAPVSPPAPVVVPTTPQTESADKPK-----------LPPKPAI-----AP-KPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR-DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P D +S P+ TP + +K L K + +P P S N IPP P + +E+S A++ S ++ + +P TCEI G +T +EI+K+K MGLGLSIVGGSDT LGAI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+ DSS P KT+ L RG GLGFS+VGGFGSPHGD+PI++KTVF+ GAAA+ G LKRGDQIL+VN + L+G++H +AV ILKN G V L +LS
Sbjct: 1596 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPS----DKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPISPITSSVNLIPPRSP---------------SCSLLENSSADL-----SSSQEPAEPLPDPKTCEIKLGRETTVEITKEK------MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPP-------PEYKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVLS 2254 HSP 3 Score: 99.3673 bits (246), Expect = 8.914e-17 Identity = 150/647 (23.18%), Postives = 257/647 (39.72%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS------DASQVKPHIPILPS------SMLAKR----------AELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVD-NEVDNKGESGGDSELSESSESL----QDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNG-------------------------------GLGISLEGTVEKVDGEEQNPHH----YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVC 738
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P D Q H PS M K+ + L + + VVA+S P+T+ N ++P ++ S+ + S S Q + E L T K+ VE+ K+ GLG++I G + L I + V AA G + DQI +V+G+ ++ +++QA+ LR T V++ + R E L I + + ++T + + + R + GV +EV G + D L + + L QDL N +++EY L + + I + KP GLG S+ G +PH +I++V G +G LK GD++L VN + L G+ H + V ILK+ V L
Sbjct: 1659 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPI--------SPITSSVN--LIPPRSPSCSLLENSSADLSSSQEPA---EPLPDPKTCEIKLGRETT--------------VEITKEKMGLGLSIVGGS--DTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMD-IMDIELFKKSTR------GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRN-------STQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPEYKTIVLHRGPEGLGFSVVGGF-------GSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2252 HSP 4 Score: 88.9669 bits (219), Expect = 1.352e-13 Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0
Query: 145 DVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-----------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
D+ I L K LG S+VG K G+++ E+ G A DG+LI+GDQIL ++GQ L N + A +L+ G+V++ + R +++S+S++ PPE + I L G GF V+ G + + +KTV + G A +DG+L GD IL +ND L G E+ +L+ + V L V
Sbjct: 2060 DIMDIELFKKSTRGLGLSIVG----RKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDL-RNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPE--------------------YKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2252 HSP 5 Score: 80.1073 bits (196), Expect = 7.337e-11 Identity = 60/196 (30.61%), Postives = 88/196 (44.90%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGA-GFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG S+VG G I + E+ P+G AA DG+L GDQI ++G+ L + +H QAI L++ V +VV R SE D + IEL G G ++ + GV + VV+ G A+ DGRL GD IL++N L+ E A +L+ + V + + R
Sbjct: 1973 EKMGLGLSIVGGSDTPLG--AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDL-RDATHEQAITALRQTSSVVKMVVYREETTSSEE----------------------DLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR 2143 HSP 6 Score: 77.7962 bits (190), Expect = 3.249e-10 Identity = 126/524 (24.05%), Postives = 218/524 (41.60%), Query Frame = 0
Query: 636 SEEYWK--NQLGSGHAILMATIQKQKPNGG-LGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKL-LGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTERMSPSVN----LPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREE----PIHEGD--IVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSAST 1140
+EE+ K +Q G +L TIQ KP G LG S+ G + + + +I+ + G G +G L+ GD++L ++G+ L + H ++IL+ V+LV AR GG + L + ++ S VS SS G+ + W+ + I L+ GLGF I+ ++ T +V+++++PGGV+ +D RL GD ++ + D+NL ++ L+ A VR+ V +P+ L V + T S S P T + S +V+ + N + D+P DDD P +G +R L + + REE P E D + LP + + KD LG+ + + E ++G+ V+S+ G +I D ++ V+G + ++ QA+++LR T G R + R A+ EE+++A+ +G ST
Sbjct: 159 AEEFQKTISQGAQGREVL--TIQLYKPEGSSLGFSVVGLRSE---QRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVAR----------------GGIPESDQLKEV-TLERSPSGVSDSSKGS-------------------DMVLNTEWAQ-VESINLVNDGSGLGFGIVGGRN------TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQA-GSRVRLIVARPVDLSFVDYK--------TLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYDDDDFAP----------NGTI---YLDRTLAQPEQDIAYLDQQEREEKTQSPREELDDEDMENLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNT----------------GKVVKLRLARYLRGAKY---EEIQQAVASGELST 593 HSP 7 Score: 77.0258 bits (188), Expect = 5.517e-10 Identity = 64/212 (30.19%), Postives = 97/212 (45.75%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT----------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRNTG-----VVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 377 HSP 8 Score: 75.8702 bits (185), Expect = 1.166e-9 Identity = 65/202 (32.18%), Postives = 94/202 (46.53%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYETL---------------DVELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E D+ LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 381 HSP 9 Score: 69.3218 bits (168), Expect = 1.237e-7 Identity = 83/332 (25.00%), Postives = 146/332 (43.98%), Query Frame = 0
Query: 235 SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQV----SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAG-YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
S+ D S+Q P E LP P+ + +E+T G G ++ G +++ V G A DGRL GD I Q+N L+ EQ LR++ + V+++V RE S + + I+ + K L +++V + F+S G + + D IL + + Y + L +V + I L G+ + S S PE +T + L + +GLG ++ G + +L IF+K+V + AA + G + DQI+ V+ +S+ G ++++AVE+L++T V L ++
Sbjct: 1938 SSADLSSSQEPAE--PLPD----PKTCEIKLGRETTVEITKEKMGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDL---MDIMDIELFKKSTR---GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR-LKPGSRRTDTCNQSKESDSSEPPPEYKT--IVLHRGPEGLGFSVVGGFGSPHGDLP-IFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVL 2253 HSP 10 Score: 67.0106 bits (162), Expect = 5.617e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 711 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 938
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842697|ref|XP_022239967.1| (multiple PDZ domain protein-like isoform X3 [Limulus polyphemus]) HSP 1 Score: 803.512 bits (2074), Expect = 0.000e+0 Identity = 534/1223 (43.66%), Postives = 707/1223 (57.81%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSE-----STQSPPETTALPSRESTPRV-LNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRL 1120
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA +DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S+ + + P + S+ S V LN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL 1170 HSP 2 Score: 588.956 bits (1517), Expect = 4.329e-170 Identity = 332/697 (47.63%), Postives = 455/697 (65.28%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS----DSPSAITAPVSPPAPVVVPTTPQTESADKPK-----------LPPKPAI-----AP-KPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR-DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P D +S P+ TP + +K L K + +P P S N IPP P + +E+S A++ S ++ + +P TCEI G +T +EI+K+K MGLGLSIVGGSDT LGAI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+ DSS P KT+ L RG GLGFS+VGGFGSPHGD+PI++KTVF+ GAAA+ G LKRGDQIL+VN + L+G++H +AV ILKN G V L +LS
Sbjct: 1598 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPS----DKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPISPITSSVNLIPPRSP---------------SCSLLENSSADL-----SSSQEPAEPLPDPKTCEIKLGRETTVEITKEK------MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPP-------PEYKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVLS 2256 HSP 3 Score: 99.3673 bits (246), Expect = 9.536e-17 Identity = 150/647 (23.18%), Postives = 257/647 (39.72%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS------DASQVKPHIPILPS------SMLAKR----------AELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVD-NEVDNKGESGGDSELSESSESL----QDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNG-------------------------------GLGISLEGTVEKVDGEEQNPHH----YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVC 738
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P D Q H PS M K+ + L + + VVA+S P+T+ N ++P ++ S+ + S S Q + E L T K+ VE+ K+ GLG++I G + L I + V AA G + DQI +V+G+ ++ +++QA+ LR T V++ + R E L I + + ++T + + + R + GV +EV G + D L + + L QDL N +++EY L + + I + KP GLG S+ G +PH +I++V G +G LK GD++L VN + L G+ H + V ILK+ V L
Sbjct: 1661 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPI--------SPITSSVN--LIPPRSPSCSLLENSSADLSSSQEPA---EPLPDPKTCEIKLGRETT--------------VEITKEKMGLGLSIVGGS--DTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMD-IMDIELFKKSTR------GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRN-------STQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPEYKTIVLHRGPEGLGFSVVGGF-------GSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2254 HSP 4 Score: 88.9669 bits (219), Expect = 1.386e-13 Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0
Query: 145 DVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-----------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
D+ I L K LG S+VG K G+++ E+ G A DG+LI+GDQIL ++GQ L N + A +L+ G+V++ + R +++S+S++ PPE + I L G GF V+ G + + +KTV + G A +DG+L GD IL +ND L G E+ +L+ + V L V
Sbjct: 2062 DIMDIELFKKSTRGLGLSIVG----RKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDL-RNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPE--------------------YKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2254 HSP 5 Score: 80.1073 bits (196), Expect = 7.589e-11 Identity = 60/196 (30.61%), Postives = 88/196 (44.90%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGA-GFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG S+VG G I + E+ P+G AA DG+L GDQI ++G+ L + +H QAI L++ V +VV R SE D + IEL G G ++ + GV + VV+ G A+ DGRL GD IL++N L+ E A +L+ + V + + R
Sbjct: 1975 EKMGLGLSIVGGSDTPLG--AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDL-RDATHEQAITALRQTSSVVKMVVYREETTSSEE----------------------DLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR 2145 HSP 6 Score: 78.9518 bits (193), Expect = 1.462e-10 Identity = 126/524 (24.05%), Postives = 220/524 (41.98%), Query Frame = 0
Query: 636 SEEYWK--NQLGSGHAILMATIQKQKPNGG-LGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKL-LGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTERMSPSVN----LPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREE----PIHEGD--IVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSAST 1140
+EE+ K +Q G +L TIQ KP G LG S+ G + + + +I+ + G G +G L+ GD++L ++G+ L + H ++IL+ V+LV AR GG + L + ++ S VS SS G+ + ++++S I L+ GLGF I+ ++ T +V+++++PGGV+ +D RL GD ++ + D+NL ++ L+ A VR+ V +P+ L V + T S S P T + S +V+ + N + D+P DDD P +G +R L + + REE P E D + LP + + KD LG+ + + E ++G+ V+S+ G +I D ++ V+G + ++ QA+++LR T G R + R A+ EE+++A+ +G ST
Sbjct: 159 AEEFQKTISQGAQGREVL--TIQLYKPEGSSLGFSVVGLRSE---QRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVAR----------------GGIPESDQLKEV-TLERSPSGVSDSSKGSDMVVGLNTEWAQVES------------------INLVNDGSGLGFGIVGGRN------TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQA-GSRVRLIVARPVDLSFVDYK--------TLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYDDDDFAP----------NGTI---YLDRTLAQPEQDIAYLDQQEREEKTQSPREELDDEDMENLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNT----------------GKVVKLRLARYLRGAKY---EEIQQAVASGELST 595 HSP 7 Score: 76.2554 bits (186), Expect = 9.690e-10 Identity = 64/214 (29.91%), Postives = 97/214 (45.33%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT------------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNT-----GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 379 HSP 8 Score: 74.3294 bits (181), Expect = 3.306e-9 Identity = 67/204 (32.84%), Postives = 96/204 (47.06%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYE-TLD----------------VELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E TL+ V LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 383 HSP 9 Score: 69.3218 bits (168), Expect = 1.207e-7 Identity = 83/332 (25.00%), Postives = 146/332 (43.98%), Query Frame = 0
Query: 235 SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQV----SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAG-YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
S+ D S+Q P E LP P+ + +E+T G G ++ G +++ V G A DGRL GD I Q+N L+ EQ LR++ + V+++V RE S + + I+ + K L +++V + F+S G + + D IL + + Y + L +V + I L G+ + S S PE +T + L + +GLG ++ G + +L IF+K+V + AA + G + DQI+ V+ +S+ G ++++AVE+L++T V L ++
Sbjct: 1940 SSADLSSSQEPAE--PLPD----PKTCEIKLGRETTVEITKEKMGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDL---MDIMDIELFKKSTR---GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR-LKPGSRRTDTCNQSKESDSSEPPPEYKT--IVLHRGPEGLGFSVVGGFGSPHGDLP-IFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVL 2255 HSP 10 Score: 67.0106 bits (162), Expect = 5.571e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 713 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 940
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842695|ref|XP_022239966.1| (multiple PDZ domain protein-like isoform X2 [Limulus polyphemus]) HSP 1 Score: 800.816 bits (2067), Expect = 0.000e+0 Identity = 534/1224 (43.63%), Postives = 705/1224 (57.60%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQ---DGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDS----ESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRL 1120
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA + DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S E T + S + + VLN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL 1171 HSP 2 Score: 588.956 bits (1517), Expect = 3.482e-170 Identity = 332/697 (47.63%), Postives = 455/697 (65.28%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS----DSPSAITAPVSPPAPVVVPTTPQTESADKPK-----------LPPKPAI-----AP-KPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR-DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P D +S P+ TP + +K L K + +P P S N IPP P + +E+S A++ S ++ + +P TCEI G +T +EI+K+K MGLGLSIVGGSDT LGAI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+ DSS P KT+ L RG GLGFS+VGGFGSPHGD+PI++KTVF+ GAAA+ G LKRGDQIL+VN + L+G++H +AV ILKN G V L +LS
Sbjct: 1599 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPS----DKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPISPITSSVNLIPPRSP---------------SCSLLENSSADL-----SSSQEPAEPLPDPKTCEIKLGRETTVEITKEK------MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPP-------PEYKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVLS 2257 HSP 3 Score: 99.3673 bits (246), Expect = 9.222e-17 Identity = 150/647 (23.18%), Postives = 257/647 (39.72%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS------DASQVKPHIPILPS------SMLAKR----------AELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVD-NEVDNKGESGGDSELSESSESL----QDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNG-------------------------------GLGISLEGTVEKVDGEEQNPHH----YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVC 738
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P D Q H PS M K+ + L + + VVA+S P+T+ N ++P ++ S+ + S S Q + E L T K+ VE+ K+ GLG++I G + L I + V AA G + DQI +V+G+ ++ +++QA+ LR T V++ + R E L I + + ++T + + + R + GV +EV G + D L + + L QDL N +++EY L + + I + KP GLG S+ G +PH +I++V G +G LK GD++L VN + L G+ H + V ILK+ V L
Sbjct: 1662 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPI--------SPITSSVN--LIPPRSPSCSLLENSSADLSSSQEPA---EPLPDPKTCEIKLGRETT--------------VEITKEKMGLGLSIVGGS--DTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMD-IMDIELFKKSTR------GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRN-------STQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPEYKTIVLHRGPEGLGFSVVGGF-------GSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2255 HSP 4 Score: 88.9669 bits (219), Expect = 1.363e-13 Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0
Query: 145 DVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-----------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
D+ I L K LG S+VG K G+++ E+ G A DG+LI+GDQIL ++GQ L N + A +L+ G+V++ + R +++S+S++ PPE + I L G GF V+ G + + +KTV + G A +DG+L GD IL +ND L G E+ +L+ + V L V
Sbjct: 2063 DIMDIELFKKSTRGLGLSIVG----RKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDL-RNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPE--------------------YKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2255 HSP 5 Score: 80.1073 bits (196), Expect = 7.156e-11 Identity = 60/196 (30.61%), Postives = 88/196 (44.90%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGA-GFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG S+VG G I + E+ P+G AA DG+L GDQI ++G+ L + +H QAI L++ V +VV R SE D + IEL G G ++ + GV + VV+ G A+ DGRL GD IL++N L+ E A +L+ + V + + R
Sbjct: 1976 EKMGLGLSIVGGSDTPLG--AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDL-RDATHEQAITALRQTSSVVKMVVYREETTSSEE----------------------DLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR 2146 HSP 6 Score: 73.1738 bits (178), Expect = 8.547e-9 Identity = 126/524 (24.05%), Postives = 216/524 (41.22%), Query Frame = 0
Query: 636 SEEYWK--NQLGSGHAILMATIQKQKPNGG-LGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKL-LGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTERMSPSVN----LPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREE----PIHEGD--IVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSAST 1140
+EE+ K +Q G +L TIQ KP G LG S+ G + E I++ G + +G L+ GD++L ++G+ L + H ++IL+ V+LV AR GG + L + ++ S VS SS G+ + W+ + I L+ GLGF I+ ++ T +V+++++PGGV+ +D RL GD ++ + D+NL ++ L+ A VR+ V +P+ L V + T S S P T + S +V+ + N + D+P DDD P +G +R L + + REE P E D + LP + + KD LG+ + + E ++G+ V+S+ G +I D ++ V+G + ++ QA+++LR T G R + R A+ EE+++A+ +G ST
Sbjct: 159 AEEFQKTISQGAQGREVL--TIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVAR----------------GGIPESDQLKEV-TLERSPSGVSDSSKGS-------------------DMVLNTEWAQ-VESINLVNDGSGLGFGIVGGRN------TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQA-GSRVRLIVARPVDLSFVDYK--------TLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYDDDDFAP----------NGTI---YLDRTLAQPEQDIAYLDQQEREEKTQSPREELDDEDMENLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNT----------------GKVVKLRLARYLRGAKY---EEIQQAVASGELST 596 HSP 7 Score: 73.1738 bits (178), Expect = 9.142e-9 Identity = 64/215 (29.77%), Postives = 98/215 (45.58%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAA---DSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT----------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A + DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRNTG-----VVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 380 HSP 8 Score: 71.633 bits (174), Expect = 2.571e-8 Identity = 65/205 (31.71%), Postives = 94/205 (45.85%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAA---HKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYETL---------------DVELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E D+ LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 384 HSP 9 Score: 69.3218 bits (168), Expect = 1.313e-7 Identity = 83/332 (25.00%), Postives = 146/332 (43.98%), Query Frame = 0
Query: 235 SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQV----SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAG-YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
S+ D S+Q P E LP P+ + +E+T G G ++ G +++ V G A DGRL GD I Q+N L+ EQ LR++ + V+++V RE S + + I+ + K L +++V + F+S G + + D IL + + Y + L +V + I L G+ + S S PE +T + L + +GLG ++ G + +L IF+K+V + AA + G + DQI+ V+ +S+ G ++++AVE+L++T V L ++
Sbjct: 1941 SSADLSSSQEPAE--PLPD----PKTCEIKLGRETTVEITKEKMGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDL---MDIMDIELFKKSTR---GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR-LKPGSRRTDTCNQSKESDSSEPPPEYKT--IVLHRGPEGLGFSVVGGFGSPHGDLP-IFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVL 2256 HSP 10 Score: 67.0106 bits (162), Expect = 5.666e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 714 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 941
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842703|ref|XP_022239970.1| (multiple PDZ domain protein-like isoform X6 [Limulus polyphemus]) HSP 1 Score: 798.505 bits (2061), Expect = 0.000e+0 Identity = 538/1249 (43.07%), Postives = 718/1249 (57.49%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQ---DGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSE-----STQSPPETTALPSRESTPRV-LNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSASTTAS 1143
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA + DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S+ + + P + S+ S V LN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L + EE+ ++ST+++
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL------QHHEESPTQATSSTSSA 1190 HSP 2 Score: 482.256 bits (1240), Expect = 9.068e-135 Identity = 273/586 (46.59%), Postives = 382/586 (65.19%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS----DSPSAITAPVSPPAPVVVPTTPQTESADKPK-----------LPPKPAI-----AP-KPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRK 2093
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P D +S P+ TP + +K L K + +P P S N IPP P + +E+S A++ S ++ + +P TCEI G +T +EI+K+K MGLGLSIVGGSDT LGAI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+
Sbjct: 1601 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPS----DKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPISPITSSVNLIPPRSP---------------SCSLLENSSADL-----SSSQEPAEPLPDPKTCEIKLGRETTVEITKEK------MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRR 2155 HSP 3 Score: 90.8929 bits (224), Expect = 3.131e-14 Identity = 69/197 (35.03%), Postives = 92/197 (46.70%), Query Frame = 0
Query: 2007 VELNKKKDRGLGLSIVGKKSGP--GVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
VE+ K+K GLGLSIVG P + I EV GAA DGRL GDQI VNG DL +++ E A L+ T + M V R + ++ + + M K+ GLG SIVG P +++ V GAA G L +GDQIL VN + L + A +LK G V ++I
Sbjct: 1973 VEITKEK-MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDI------------------MDIELFKKSTRGLGLSIVGRKNGPG----VFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKI 2146 HSP 4 Score: 85.8853 bits (211), Expect = 1.154e-12 Identity = 100/422 (23.70%), Postives = 177/422 (41.94%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS------DASQVKPHIPILPS------SMLAKR----------AELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVR 553
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P D Q H PS M K+ + L + + VVA+S P+T+ N ++P ++ S+ + S S Q + E L T K+ VE+ K+ GLG++I G + L I + V AA G + DQI +V+G+ ++ +++QA+ LR T V++ + R
Sbjct: 1664 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPI--------SPITSSVN--LIPPRSPSCSLLENSSADLSSSQEPA---EPLPDPKTCEIKLGRETT--------------VEITKEKMGLGLSIVGGS--DTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYR 2053 HSP 5 Score: 80.1073 bits (196), Expect = 6.318e-11 Identity = 60/196 (30.61%), Postives = 88/196 (44.90%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGA-GFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG S+VG G I + E+ P+G AA DG+L GDQI ++G+ L + +H QAI L++ V +VV R SE D + IEL G G ++ + GV + VV+ G A+ DGRL GD IL++N L+ E A +L+ + V + + R
Sbjct: 1978 EKMGLGLSIVGGSDTPLG--AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDL-RDATHEQAITALRQTSSVVKMVVYREETTSSEE----------------------DLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR 2148 HSP 6 Score: 72.4034 bits (176), Expect = 1.304e-8 Identity = 64/217 (29.49%), Postives = 98/217 (45.16%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAA---DSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT------------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A + DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNTG-----VVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 382 HSP 7 Score: 72.0182 bits (175), Expect = 2.019e-8 Identity = 65/220 (29.55%), Postives = 105/220 (47.73%), Query Frame = 0
Query: 1994 IHRE-DDEIYETLDVELNKKKDRGLGLSIVGKK--SGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR---------DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQI 2201
IH++ D E + VEL +K GLGLS+ G K S VFI + G A DGR+ GD+++ VNG + + + I+K ++ V ++ + R+ V M + + P G ++ V +K+G GLG I+ G + G I++ + + + A+ GL GD ILSVN + L G + A +ILK +G + L +
Sbjct: 1643 IHKKYSDSKGELVVVEL-QKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIK----NLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRG-VRVVTVKKGNQGLGIMIIEGKHAEVG-QGIFISDIQEN-SVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVV 1854 HSP 8 Score: 70.0922 bits (170), Expect = 6.775e-8 Identity = 67/207 (32.37%), Postives = 96/207 (46.38%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAA---HKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYE-TLD----------------VELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E TL+ V LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 386 HSP 9 Score: 67.3958 bits (163), Expect = 4.926e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 716 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 943
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842693|ref|XP_022239965.1| (multiple PDZ domain protein-like isoform X1 [Limulus polyphemus]) HSP 1 Score: 798.119 bits (2060), Expect = 0.000e+0 Identity = 534/1226 (43.56%), Postives = 707/1226 (57.67%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQ---DGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSE-----STQSPPETTALPSRESTPRV-LNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRL 1120
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA + DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S+ + + P + S+ S V LN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL 1173 HSP 2 Score: 588.956 bits (1517), Expect = 4.466e-170 Identity = 332/697 (47.63%), Postives = 455/697 (65.28%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKS----DSPSAITAPVSPPAPVVVPTTPQTESADKPK-----------LPPKPAI-----AP-KPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR-DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P D +S P+ TP + +K L K + +P P S N IPP P + +E+S A++ S ++ + +P TCEI G +T +EI+K+K MGLGLSIVGGSDT LGAI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+ DSS P KT+ L RG GLGFS+VGGFGSPHGD+PI++KTVF+ GAAA+ G LKRGDQIL+VN + L+G++H +AV ILKN G V L +LS
Sbjct: 1601 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPS----DKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPISPITSSVNLIPPRSP---------------SCSLLENSSADL-----SSSQEPAEPLPDPKTCEIKLGRETTVEITKEK------MGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPP-------PEYKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVLS 2259 HSP 3 Score: 99.3673 bits (246), Expect = 8.919e-17 Identity = 150/647 (23.18%), Postives = 257/647 (39.72%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS------DASQVKPHIPILPS------SMLAKR----------AELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVD-NEVDNKGESGGDSELSESSESL----QDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNG-------------------------------GLGISLEGTVEKVDGEEQNPHH----YIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVC 738
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P D Q H PS M K+ + L + + VVA+S P+T+ N ++P ++ S+ + S S Q + E L T K+ VE+ K+ GLG++I G + L I + V AA G + DQI +V+G+ ++ +++QA+ LR T V++ + R E L I + + ++T + + + R + GV +EV G + D L + + L QDL N +++EY L + + I + KP GLG S+ G +PH +I++V G +G LK GD++L VN + L G+ H + V ILK+ V L
Sbjct: 1664 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKNTPPPPPPKPSHLSSKMSFQVVAHSPI--------SPITSSVN--LIPPRSPSCSLLENSSADLSSSQEPA---EPLPDPKTCEIKLGRETT--------------VEITKEKMGLGLSIVGGS--DTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMD-IMDIELFKKSTR------GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRN-------STQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPEYKTIVLHRGPEGLGFSVVGGF-------GSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2257 HSP 4 Score: 88.9669 bits (219), Expect = 1.387e-13 Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0
Query: 145 DVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVAR-----------GSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAG----VVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIV 347
D+ I L K LG S+VG K G+++ E+ G A DG+LI+GDQIL ++GQ L N + A +L+ G+V++ + R +++S+S++ PPE + I L G GF V+ G + + +KTV + G A +DG+L GD IL +ND L G E+ +L+ + V L V
Sbjct: 2065 DIMDIELFKKSTRGLGLSIVG----RKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDL-RNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPPE--------------------YKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTV 2257 HSP 5 Score: 80.1073 bits (196), Expect = 7.591e-11 Identity = 60/196 (30.61%), Postives = 88/196 (44.90%), Query Frame = 0
Query: 155 EGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGA-GFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAR 349
E LG S+VG G I + E+ P+G AA DG+L GDQI ++G+ L + +H QAI L++ V +VV R SE D + IEL G G ++ + GV + VV+ G A+ DGRL GD IL++N L+ E A +L+ + V + + R
Sbjct: 1978 EKMGLGLSIVGGSDTPLG--AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDL-RDATHEQAITALRQTSSVVKMVVYREETTSSEE----------------------DLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR 2148 HSP 6 Score: 74.3294 bits (181), Expect = 3.627e-9 Identity = 126/524 (24.05%), Postives = 218/524 (41.60%), Query Frame = 0
Query: 636 SEEYWK--NQLGSGHAILMATIQKQKPNGG-LGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKL-LGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTERMSPSVN----LPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREE----PIHEGD--IVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSAST 1140
+EE+ K +Q G +L TIQ KP G LG S+ G + E I++ G + +G L+ GD++L ++G+ L + H ++IL+ V+LV AR GG + L + ++ S VS SS G+ + ++++S I L+ GLGF I+ ++ T +V+++++PGGV+ +D RL GD ++ + D+NL ++ L+ A VR+ V +P+ L V + T S S P T + S +V+ + N + D+P DDD P +G +R L + + REE P E D + LP + + KD LG+ + + E ++G+ V+S+ G +I D ++ V+G + ++ QA+++LR T G R + R A+ EE+++A+ +G ST
Sbjct: 159 AEEFQKTISQGAQGREVL--TIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVAR----------------GGIPESDQLKEV-TLERSPSGVSDSSKGSDMVVGLNTEWAQVES------------------INLVNDGSGLGFGIVGGRN------TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQA-GSRVRLIVARPVDLSFVDYK--------TLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYDDDDFAP----------NGTI---YLDRTLAQPEQDIAYLDQQEREEKTQSPREELDDEDMENLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNT----------------GKVVKLRLARYLRGAKY---EEIQQAVASGELST 598 HSP 7 Score: 72.4034 bits (176), Expect = 1.606e-8 Identity = 64/217 (29.49%), Postives = 98/217 (45.16%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAA---DSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT------------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A + DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNTG-----VVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 382 HSP 8 Score: 70.0922 bits (170), Expect = 7.351e-8 Identity = 67/207 (32.37%), Postives = 96/207 (46.38%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAA---HKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYE-TLD----------------VELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E TL+ V LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 386 HSP 9 Score: 69.3218 bits (168), Expect = 1.227e-7 Identity = 83/332 (25.00%), Postives = 146/332 (43.98%), Query Frame = 0
Query: 235 SAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQV----SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAG-YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
S+ D S+Q P E LP P+ + +E+T G G ++ G +++ V G A DGRL GD I Q+N L+ EQ LR++ + V+++V RE S + + I+ + K L +++V + F+S G + + D IL + + Y + L +V + I L G+ + S S PE +T + L + +GLG ++ G + +L IF+K+V + AA + G + DQI+ V+ +S+ G ++++AVE+L++T V L ++
Sbjct: 1943 SSADLSSSQEPAE--PLPD----PKTCEIKLGRETTVEITKEKMGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDL---MDIMDIELFKKSTR---GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR-LKPGSRRTDTCNQSKESDSSEPPPEYKT--IVLHRGPEGLGFSVVGGFGSPHGDLP-IFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVL 2258 HSP 10 Score: 67.0106 bits (162), Expect = 5.572e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 716 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 943
BLAST of conserved hypothetical protein vs. nr
Match: gi|1238842701|ref|XP_022239969.1| (multiple PDZ domain protein-like isoform X5 [Limulus polyphemus]) HSP 1 Score: 798.119 bits (2060), Expect = 0.000e+0 Identity = 534/1226 (43.56%), Postives = 707/1226 (57.67%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVN-GDLILNL-PDGQALASPVH-SDPPAPRSPPS--------PLETAMA----------PRLEN------------------EPRFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQ---DGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSE-----STQSPPETTALPSRESTPRV-LNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPS--DASQVKPHIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQV--SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSELSESSESLQDLENQPEIFSETSEEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQ--------STSVGTKQDFPLGGTFRHEILANT-----------------------ERMVKAKSDVSIS-SAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVS----EQYDED----DSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISAR-----------------FVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQVDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRL 1120
MPI+ D R AL LL+ + L S A +T T+ +DL TLI+VLE+PVF +IL IQDSL ELK+Q+ HPSILP DFDI + G+LILN+ PD SP+H SD S P+E +A P L+ FQ I Q +QGR+V I L KPEG SLGFSVVGL+SE + ELGI++QEIQ GIA + DG+L GDQILAIDGQPLDSNISH QAI+ILQ+ARG V LVVARG +S+ + + P + S+ S V LN++W QVE I L N+G+G GFG++ G+ GVVVKT++ GVAD+D RL +GD+ILQI D L+G EQVA+VLR++G+ VRLIVAR VD S D + PI+P+ +L+ E++ L + ++ + +D P T + ++ QP +DQ QE + R ++ M ++PEMET +VEL KD QGLG+TIAGY CE+EELSGIFVKS+ GSAA S ++VNDQIIEVDG+ + G++N QAVE+LR+TG+ V+LRL RY+ G K+E++QQA+A+ + ++ P A T SSP V N V N + D + + + L+ E + W +G G I++A + K P GGLGISLEGTV+ +G+E PHHYIRS+L +GPVG NG L+SGDELLEVNG+KLLGL + VVAILK+LP+ V++VCAR STSV + R A T ER+VKAKSD S++ A T + + + SK++SRSLEPLT LAMW+ IEL+KG+RGLGFSILDYQDPMN +ETVIVIRSLVPGGV+QQDGRLIPGDRL+ VNDINLE+ASLD AVQALKGAP+G+VRI V KPLPLPE S E +DE D S P+ +++ M S + P + +P LP++ D + L V+ + F E PI V LP+ALERT ++KD+ G+ ++ D GING++V S++ G + R +I GD+LVA+N +R A S+A IL R ++ ++ + YIPA DAAV+R + + L
Sbjct: 1 MPISGDTRQALKLLERVQQQLQDSDEASLNT---TVGEDLNTLINVLENPVFSSILTIQDSLQELKNQLHQHPSILPGDFDISTSTGELILNVSPD----PSPLHNSDSYDLNSDLEQNYEFDQVPIEKVLATGNGDYQDTTPELDQPQLVPPDYTLSSIATDSYAEEFQKTISQGAQGREVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNT-GVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVARPVDLSFVDYKTLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYD--------DDDFAPNGTIYLDRTLAQPEQDIAYLDQ-----QEREEKTQSPREELDDEDME----NLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNTGKVVKLRLARYLRGAKYEEIQQAVASGELSTPVPPP-----APVITQYTNSSPYEVINAV-NSEVNIDDISILINDDYTGSLDASTEAVIKAK---WNKIMGPGFDIVVAQLSKFHPGGGLGISLEGTVDVEEGKEIRPHHYIRSILSDGPVGLNGKLQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCLPSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKGDRGLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGVVRISVAKPLPLPESSQGNQEGFDESGRVIDHPRKASLDLPF---------LSSHEMLGSSDQP-------SSLPSTLPSTVERRHVVLDLTSTSHKLRNVLPDVLHSSEEKTLPSEPSSEESCQAFTPPESPILSN--VYTLPSALERTAHIKKDNLPFGLGIEAIDSGINGMMVTSLSKDGAVHRHSQIQEGDFLVALNNISLRNADISEARVILERAWN-DISTEVTLYYIPATDAAVHRQTVLMTL 1173 HSP 2 Score: 540.806 bits (1392), Expect = 5.161e-154 Identity = 312/676 (46.15%), Postives = 427/676 (63.17%), Query Frame = 0
Query: 1538 GQTTLSNGLVIDKNSAA---FLPKGNHEG-EEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILKGEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRE-----DDEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSR-DSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
G+ G+ I++ SA PK E E+ED+FGY+T+KI KKY+ +KG + V L KG++GLG+SLAG+KDRSKM+V+ICG++P GNA D R++VGD +LEVNG V+ + HLNVS+ IKNL + ILLR +++++ +AVKPL +P + ++ P ++YS +Y+G+ V++ KG QGLGIMIIEGKHA+VG G+F+SD+Q S A+ AGL GDMIL+VN + VG +Y++AA ILK +EG I+++V+NP P S +AI + + +T S DK + KPA P+ P K + + TTVE +K K G I G+DT P+G AI IHEVY +GAA DGRLKPGDQI +V EDLR+ +H A+ ALRQT + V+++++RE ++++ + +D+EL KK RGLGLSIVG+K+GPGVFISEVVKGGAA++DGRL+QGDQI+ VNG DL NS+QE AA +LK T GK+ M++ RL+ G+R+ DSS P KT+ L RG GLGFS+VGGFGSPHGD+PI++KTVF+ GAAA+ G LKRGDQIL+VN + L+G++H +AV ILKN G V L +LS
Sbjct: 1601 GRVFTKQGVEIERASAGNVKLTPKEKGEDPEKEDQFGYTTKKIHKKYSDSKGELVVVELQKGSHGLGLSLAGNKDRSKMNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYS-QYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAGLTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRP---QSVAAIIDEEQKSEHSISEPSDKTPS-DKMQEKKKPA-------------EPLPDP------KTCEIKLGRETTVEITK-----------EKMG------LGLSIVGGSDT-------------PLG--------------AIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDLMDIMDIELFKKSTRGLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGRLKPGSRRTDTCNQSKESDSSEPPP-------EYKTIVLHRGPEGLGFSVVGGFGSPHGDLPIFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVLS 2201 HSP 3 Score: 91.6633 bits (226), Expect = 1.961e-14 Identity = 110/450 (24.44%), Postives = 199/450 (44.22%), Query Frame = 0
Query: 138 QQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVA----------------RGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEV-----IELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQV----SIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAG-YTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLV 552
QQ SQ R V+ + + K LG ++ K G+ GI++ +IQ +A Q G L GD IL+++ + L + A +IL++A G + LVV+ + SE + P ++ + + C++++ +E+T G G ++ G +++ V G A DGRL GD I Q+N L+ EQ LR++ + V+++V RE S + + I+ + K L +++V + F+S G + + D IL + + Y + L +V + I L G+ + S S PE +T + L + +GLG ++ G + +L IF+K+V + AA + G + DQI+ V+ +S+ G ++++AVE+L++T V L ++
Sbjct: 1765 QQYSQYRGVRVVTVKKG-NQGLGIMIIEGKHAEVGQ-GIFISDIQENSVAEQAG-LTVGDMILSVNQREL-VGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAIIDEEQKSEHSISEPSDKTPSDKMQEKKKPAEPLPDPKTCEIKLGRETTVEITKEKMGLGLSIVGGSDTPLGAIIIHEVYPEGAAALDGRLKPGDQIHQVNGEDLRDATHEQAITALRQTSSVVKMVVYREETTSSEEDL---MDIMDIELFKKSTR---GLGLSIVGRKNGPGVFISEVVKGGAAEADGRLIQGDQILEVNGQDLRNSTQEYAATLLKTTVGKVNMKIGR-LKPGSRRTDTCNQSKESDSSEPPPEYKT--IVLHRGPEGLGFSVVGGFGSPHGDLP-IFIKTVFEKGAAAEDGQLKRGDQILAVNDQSLDGVTHEEAVEILKNTKGVVTLTVL 2200 HSP 4 Score: 74.3294 bits (181), Expect = 3.799e-9 Identity = 126/524 (24.05%), Postives = 218/524 (41.60%), Query Frame = 0
Query: 636 SEEYWK--NQLGSGHAILMATIQKQKPNGG-LGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKL-LGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNS--SDVNTERMSPSVN----LPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREE----PIHEGD--IVPPLPAALERTIVVRKDSDTLGIQVD---IQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRTHMIPLNEKIQITYIPAGDAAVYRASTITRLAEARAEEEMEEAMGAGSAST 1140
+EE+ K +Q G +L TIQ KP G LG S+ G + E I++ G + +G L+ GD++L ++G+ L + H ++IL+ V+LV AR GG + L + ++ S VS SS G+ + ++++S I L+ GLGF I+ ++ T +V+++++PGGV+ +D RL GD ++ + D+NL ++ L+ A VR+ V +P+ L V + T S S P T + S +V+ + N + D+P DDD P +G +R L + + REE P E D + LP + + KD LG+ + + E ++G+ V+S+ G +I D ++ V+G + ++ QA+++LR T G R + R A+ EE+++A+ +G ST
Sbjct: 159 AEEFQKTISQGAQGREVL--TIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVAR----------------GGIPESDQLKEV-TLERSPSGVSDSSKGSDMVVGLNTEWAQVES------------------INLVNDGSGLGFGIVGGRN------TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQA-GSRVRLIVARPVDLSFVDYK--------TLSQSAPIVPTHILSDPDEVDHQLALFQQNQKGYIEDIPYDDDDFAP----------NGTI---YLDRTLAQPEQDIAYLDQQEREEKTQSPREELDDEDMENLPEMETFEVELTKDQQGLGVTIAGYVCEKEELSGIFVKSIAKGSAADISKQILVNDQIIEVDGRPLHGYTNHQAVEVLRNT----------------GKVVKLRLARYLRGAKY---EEIQQAVASGELST 598 HSP 5 Score: 72.0182 bits (175), Expect = 1.973e-8 Identity = 64/217 (29.49%), Postives = 98/217 (45.16%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAA---DSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGT------------MKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E L ++L K + LG S+VG +S G+FI E+ G A + DGRL GDQI+ ++G L +N S + A IL+ +G + + V R + Q R PS V ++ G+ ++++ L GLGF IVGG + + VKT+ G A L+ GD IL + L G+ ++L+ V L
Sbjct: 174 EVLTIQLYKPEGSSLGFSVVGLRSEQRKELGIFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERS---PSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRNTG-----VVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRL 382 HSP 6 Score: 70.8626 bits (172), Expect = 3.931e-8 Identity = 50/206 (24.27%), Postives = 95/206 (46.12%), Query Frame = 0
Query: 157 HSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSRESTPRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQA---AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQV 359
H LG S+ G K K + +++ + P+G AA DG++ GD++L ++G + + + I + +++ R E P + E TP S + V V+ + G G ++ G+ A G+ + + +N VA+Q G L GD IL +N L G + A++L+++ ++L+V+ P + +
Sbjct: 1664 HGLGLSLAGNKDRSK--MNVFICGMNPQGNAACDGRIKVGDELLEVNGLVMQGKCHLNVSAIIKNLSEPVYKIILLRRENAVEEMAVKPLVHYPVDLEEETPEQQYSQYRGVRVVTVKKGNQGLGIMIIEGKHAEVGQGIFISDIQENSVAEQAG-LTVGDMILSVNQRELVGADYDTAASILKQAEGTIQLVVSNPTRPQSVAAI 1866 HSP 7 Score: 70.0922 bits (170), Expect = 8.099e-8 Identity = 67/207 (32.37%), Postives = 96/207 (46.38%), Query Frame = 0
Query: 1921 KPMG--LGLSIVG---GSDTLLGAIFIHEVYEKGAA---HKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHR----EDDEIYE-TLD----------------VELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
KP G LG S+VG LG IFI E+ G A +DGRL+ GDQIL + + L N+SH A+ L+Q V L++ R E D++ E TL+ V LN + + GLG IVG ++ GV + ++ GG AD D RL GD I+ + +L E A +L+ ++ + V R
Sbjct: 182 KPEGSSLGFSVVGLRSEQRKELG-IFIQEIQNTGIAGRIERDGRLQVGDQILAIDGQPLDSNISHQQAISILQQARGLVELVVARGGIPESDQLKEVTLERSPSGVSDSSKGSDMVVGLNTEWAQVESINLVNDGSGLGFGIVGGRN-TGVVVKTILPGGVADRDKRLQSGDHILQIGDVNLRGMRSEQVASVLRQAGSRVRLIVAR 386 HSP 8 Score: 67.3958 bits (163), Expect = 4.935e-7 Identity = 68/245 (27.76%), Postives = 103/245 (42.04%), Query Frame = 0
Query: 1751 LQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQ--TESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTL---LGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRV 1990
LQ GD +L VNG+ +G++Y ILK ++M+ A + P ++D ++ VS P + V + TE+ P+ S + + A G A G + A T+ESS + +R P +S P E+ G D+ GLG SI+ D + I I + G A +DGRL PGD++L V +L N S A+ AL+ P V
Sbjct: 716 LQSGDELLEVNGQKLLGLSYVQVVAILKELPVHVRMVCARCL-PSETDDILPLSTSVSDPLVLQVNALNRDYTEAMTSSMTMDSPSQHLSGSLTSLTPLSERLVKAKSDGSLAVGASTASAITLESSFSKVRSRSLEPLTGLAMWTSEPQIIELVKG---------DR-------GLGFSILDYQDPMNPNETVIVIRSLVPGGVAQQDGRLIPGDRLLSVNDINLENASLDTAVQALKGAPKGV 943
BLAST of conserved hypothetical protein vs. nr
Match: gi|1101343834|ref|XP_018897604.1| (PREDICTED: multiple PDZ domain protein-like isoform X3 [Bemisia tabaci]) HSP 1 Score: 781.171 bits (2016), Expect = 0.000e+0 Identity = 471/982 (47.96%), Postives = 623/982 (63.44%), Query Frame = 0
Query: 4 MPITHDARAALALLKEAKVDLGPSAGAPGSTDEVTLRQDLATLISVLESPVFQAILNIQDSLGELKHQVQLHPSILPSDFDIGVNGDLILNLPDGQA-------LASPVHSDPPAPR-SPPSPLETAMAPRLENEP-----------RFQALIQQASQGRDVKHIALSKPEGHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVARGSAEDSESTQSPPETTALPSREST--PRVLNSDWCQVEVIELTNNGAGFGFGVMAGQQAAGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKP---HIPILPSSMLAKRAELETHLQIAVVANSSFVSAASHGPPPLTNLANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEELLMGTTARSKMHSSLMGVSQGDM---------PEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVTDGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRLVRYVHGLKFEQLQQAIANSQSNSQATTPSVSQVAVSATPLARSSPQGVDNEVDNKGESGGDSE---LSESSESLQDLENQPEIFSETS------------EEYWKNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPMTVKLVCARSQSQSTSVGTKQDFP-------LGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPE-VSEQYDEDDSAHTESSSTPY 929
MP+ D AL LL+ + ++ S G P + + L LIS LE+P+ + I +QD++ EL Q+Q HPS+LP DFDI + GDL+L P A + P D P+ SP S E P ++ FQ I++++QGR++ I L KPEG SLGFSVV L+SE KGELGI+VQEIQPKGIA +DG+L EGDQILAIDGQPLD+NISH QA +ILQKARGRV LVVAR + SP + + ++ T VLN++W QVEVI+L N+G+G GFG++ G+ + GVVVKT+++ GVA++DGRL +GD+ILQI L+G G EQVA VLR++G+HVRLIVAR VD + A+ + P H PI+P+ +LA EL+ HL + N ++ +I +P ++ +SP++ +L + T + + V DM P+ E V L KD GLGITIAGY CE+EELSGIFVKS+++GSAA + VND+I+EVDGKS+QG++N QAVE+LRSTG++V+L L RY+ G KFEQLQQAIANSQ + T PS S +++ P+ G E+D++GES + L E++ + DLE+ + + + W LG I++ + K GGLGISLEGTV+ DG+E PHHYIRS+LP GPVG NG LKSGDELLEVNG +LLG+ H +VV+ILK+LP+ V++VCAR + ++ + T QD LGG+ ++ I A +R+VKAKSD S++S+ TVT P SKLKSRSLEPLT LAMWS IEL+KGERGLGFSILDYQDPMN E+VIVIRSLVPGGV+Q DGRLIPGDRL+ VN NLE+ASLD+AVQALKGAP+G+V+IGV KPLP+P+ VS+ +DD+ T + Y
Sbjct: 1 MPLNSDISTALQLLEHIQSEIEKS-GDPKLQSQTN--EYLMFLISTLENPILRTIAYVQDAVSELNEQLQQHPSLLPGDFDISLAGDLVLGTPSSPAHFTSSNLVTPPELDDQCVPQTSPLSSTEQIATPESKDVTLTPITTASYILEFQKAIEESAQGREIITIQLMKPEGGSLGFSVVSLRSEDKGELGIFVQEIQPKGIAGRDGRLHEGDQILAIDGQPLDTNISHQQAFSILQKARGRVELVVARTESPSPPPLNSPSRSPSPSNKSETGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGR-STGVVVKTILRGGVAERDGRLQSGDHILQIGGVSLRGMGREQVAAVLRQAGSHVRLIVARPVDAA-ATDLHPLGSHAPIVPTKLLADAEELDKHL----IQNGYSTTS------------------------NILEPYNI------FSSPLESDLDLNETGLIVDVAPSLPVLTMDMIPPESLQPLPDTEKFTVVLKKDVHGLGITIAGYVCEKEELSGIFVKSISEGSAADLCKKIQVNDRIVEVDGKSLQGYTNHQAVEVLRSTGQTVKLCLERYLRGPKFEQLQQAIANSQLKA-PTPPSPSALSLPRVPIMND---GETCEIDHEGESQDTVDSIALFEANNADVDLESIEMLIDDDYSGPLKPTVEAAIQAKWSKILGPDVDIVVGQLCKFGEGGGLGISLEGTVDVEDGQEVRPHHYIRSILPEGPVGRNGRLKSGDELLEVNGERLLGMNHVEVVSILKELPINVRMVCARRKGENRLIDTSQDRAAFAARSLLGGSLQNLIPA-MDRLVKAKSDGSLASSATVTTD---PSLSKLKSRSLEPLTGLAMWSSEPHIIELMKGERGLGFSILDYQDPMNPSESVIVIRSLVPGGVAQLDGRLIPGDRLLSVNSTNLENASLDQAVQALKGAPKGVVKIGVAKPLPIPDSVSQSQGDDDATPTLDDHSMY 935 HSP 2 Score: 549.28 bits (1414), Expect = 1.748e-156 Identity = 323/664 (48.64%), Postives = 414/664 (62.35%), Query Frame = 0
Query: 1564 EEEDEFGYS---TRKILKKYARAKGTPIYVSLNKGTNGLGISLAGHKDRSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRSDSSIDNLAVKPLSQFPPEPHKDNPIERYSGKYKGLHEVSILK---------------GEQGLGIMIIEGKHADVGSGVFVSDLQPGSCADVAGLQRGDMILAVNGEDFVGVNYESAARILKNSEGLIKMIVANPIGPVKSDSPSAITAPVSPPAPVVVPTTPQTESADKPKLPPKPAIAPKPGAVSPNHIPPIVPPAAGGGGKASGVVASKATTVESSKANIRKPVASPRRKAGDES--SNPATCEITPGADTVIEISKDKDENGKPMGLGLSIVGGSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDLRNVSHSHALHALRQTPNRVRLMIHRED----DEIYETLDVELNKKKDRGLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSNVPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVMLQILS 2203
E+EDEFGY+ K+ KKYA VS+ +G GLGISLAGHKDR+KM+ +ICGL+P+G AF+ ++VGD ILEVNG V+ R HLN S+ IK L ILLR +++ LAVKP++QFP + P E+++ +KGL V+I K QGLGIMIIEGKHA+VG G+F+SD+Q GS A+ AGL GDMILAVN + +G NY+SAA +LK +EG++ ++V NP K D A DKPK P KP + H+ PI P + E S A + P S + AG E ++PAT I PGA++VIEI+KDK MGLGLSIVGGSDTLLGAI IHEVY GAA KDGRL PGD ILE ED RN++HS AL ALRQTP +V++ + R+D ++ E +DVELNKK +GLGLSIVG+K+GPG+FISEVV GGAA+ DGRL++GDQI++VNG DL +SQE+AA +LK T GK+ ++++G RK + S D+ + T GT++TV L+RG GLGFSIVGG SPHG++PIYVKTVF +G LKRGDQI++V+ LEGL+H AV+ILK G V L I S
Sbjct: 1731 EQEDEFGYTLTEINKVKKKYAAHGDAVCIVSVERGHQGLGISLAGHKDRTKMAAFICGLNPNGPAFKSGVVKVGDEILEVNGVVLRGRCHLNASAIIKGLAGPTYKIILLRRSDALEELAVKPITQFPVSLDDETPEEKFA-HFKGLKIVTIKKPGYVEAPPAPPSPQVAGQGLGIMIIEGKHAEVGQGIFISDIQEGSAAEQAGLIVGDMILAVNKDTILGNNYDSAASLLKKTEGVVTLVVCNP-QKAKEDEKKA--------------------GGDKPKEPEKPRL----------HLKPIAPSS------------------EMSTARL-SPSPSGKIHAGTEEPPADPATAPIKPGAESVIEINKDK------MGLGLSIVGGSDTLLGAIIIHEVYPDGAAAKDGRLLPGDVILECNSEDFRNITHSKALAALRQTPAKVKMTVLRDDVAKEEDKLEIIDVELNKKPGKGLGLSIVGRKNGPGIFISEVVSGGAANVDGRLMKGDQILSVNGRDLKTASQEEAAAVLKTTSGKV-----QIKLGRRKAKLSAATS-DNENEDSTGEETEGTLRTVTLERGPDGLGFSIVGGACSPHGNLPIYVKTVF----FDLNGLLKRGDQIVAVDGLSLEGLTHQQAVDILKKAQGVVTLTIQS 2327 HSP 3 Score: 98.5969 bits (244), Expect = 1.805e-16 Identity = 129/495 (26.06%), Postives = 206/495 (41.62%), Query Frame = 0
Query: 1357 QGLGISIVGGKVEPNAPKDGSSPGQNPPVVTGIFIKNVLEGSPAGLSDNLFTGDRILAVDGHDLSCATHDQAVDVIRQSGEVVEFVVQSLLGVTAASSTQADSPTHSDMSESVATS-LPPPPPPPPLIDSVPPGFANDPVPTPVMTGSEDSATEIPGDDGQSSSSSSCSSD-EDEYEDSGHYMGQTTLSNGLVIDKNSAAFLPKGNHEGEEEDEFGYSTRKILKKYARAKGTPIYVSLNKGTN-----GLGISLAGHKD-----RSKMSVYICGLHPHGNAFRDARMQVGDLILEVNGHVIYDRHHLNVSSFIKNLPDSDVTFILLRS-------DSSIDNLAVKPLSQFPPE-----PHKDNPIERYS----------------GKYK--------GL-------HEVSILKGEQGLGIMIIEGK--HADVGSGVFVSDLQPGSCADVAG-LQRGDMILAVNGEDFVGVNYESAARILKNS-EGLIKMIVANPI 1792
GLGI+I G E ++GIF+K++ EGS A L + DRI+ VDG L T+ QAV+V+R +G+ V+ ++ L P + +++A S L P PP P S+P P+M +DG+ +C D E E +D+ + +N V D S L ++ G + + I K+++ G + + + + GLGISL G D + YI + P G R+ R++ GD +LEVNG + +H+ V S +K LP +V + R D+S D A S P D ++ S K K GL H + ++KGE+GLG I++ + S + + L PG A + G L GD +L+VN + + + A + LK + +G++K+ VA P+
Sbjct: 469 HGLGITIAGYVCEKEE-------------LSGIFVKSISEGSAADLCKKIQVNDRIVEVDGKSLQGYTNHQAVEVLRSTGQTVKLCLERYL----------RGPKFEQLQQAIANSQLKAPTPPSPSALSLP--------RVPIM------------NDGE-----TCEIDHEGESQDTVDSIALFEANNADV-DLESIEMLIDDDYSGPLKPTVEAA---IQAKWSKILGPDVDIVVGQLCKFGEGGGLGISLEGTVDVEDGQEVRPHHYIRSILPEGPVGRNGRLKSGDELLEVNGERLLGMNHVEVVSILKELP-INVRMVCARRKGENRLIDTSQDRAAFAARSLLGGSLQNLIPAMDRLVKAKSDGSLASSATVTTDPSLSKLKSRSLEPLTGLAMWSSEPHIIELMKGERGLGFSILDYQDPMNPSESVIVIRSLVPGGVAQLDGRLIPGDRLLSVNSTNLENASLDQAVQALKGAPKGVVKIGVAKPL 910 HSP 4 Score: 83.5741 bits (205), Expect = 6.501e-12 Identity = 112/446 (25.11%), Postives = 188/446 (42.15%), Query Frame = 0
Query: 156 GHSLGFSVVGLKSEHKGELGIYVQEIQPKGIAAQDGQLIEGDQILAIDGQPLDSNISHHQAINILQKARGRVHLVVA--RGSAEDSESTQ----SPPETTAL------PSRE-STPRVLNSDWCQVE---------------------VIELTNNGAGFGFGVMAGQQA--AGVVVKTVVQNGVADQDGRLHAGDYILQINDHWLQGFGIEQVANVLRKSGNHVRLIVAREVDPSDASQVKPHIPILPSSMLAKRAELETHLQIAVVANSS----FVSAASHGPPPLTN---LANDMILPTQTEQVSIYQPSHLPKSVDQTTSPIQEEL------LMGTTARSKMHSSLMGVSQGDMPEMETLDVELVKDAQGLGITIAGYTCEREELSGIFVKSVT-DGSAAFKSGMVVVNDQIIEVDGKSIQGFSNQQAVEMLRSTGESVRLRL 551
G LG ++ K G+ GI++ +IQ +G AA+ LI GD ILA++ + N ++ A ++L+K G V LVV + + ED + PE L PS E ST R+ S ++ VIE+ + G G ++ G +++ V +G A +DGRL GD IL+ N + + LR++ V++ V R+ D ++ + + I+ + K + L +++V + F+S G + + D IL + + T+ +Q +L L T+ ++ S G+ E V L + GLG +I G C I+VK+V D + K G DQI+ VDG S++G ++QQAV++L+ V L +
Sbjct: 1900 GQGLGIMIIEGKHAEVGQ-GIFISDIQ-EGSAAEQAGLIVGDMILAVNKDTILGN-NYDSAASLLKKTEGVVTLVVCNPQKAKEDEKKAGGDKPKEPEKPRLHLKPIAPSSEMSTARLSPSPSGKIHAGTEEPPADPATAPIKPGAESVIEINKDKMGLGLSIVGGSDTLLGAIIIHEVYPDGAAAKDGRLLPGDVILECNSEDFRNITHSKALAALRQTPAKVKMTVLRD----DVAKEEDKLEIIDVELNKKPGK---GLGLSIVGRKNGPGIFISEVVSGGAANVDGRLMKGDQILSVNGRDLKTASQEEAAAVLKTTSGKVQIKLGRRKAKLSAATSDNENEDS-----TGEETEGTLRTVTLERGPDGLGFSIVGGACSPHGNLPIYVKTVFFDLNGLLKRG-----DQIVAVDGLSLEGLTHQQAVDILKKAQGVVTLTI 2325 HSP 5 Score: 80.1073 bits (196), Expect = 7.630e-11 Identity = 119/454 (26.21%), Postives = 179/454 (39.43%), Query Frame = 0
Query: 641 KNQLGSGHAILMATIQKQKPNGGLGISLEGTVEKVDGEEQNPHHYIRSVLPNGPVGANGVLKSGDELLEVNGRKLLGLYHTDVVAILKDLPM-TVKLVCARSQSQSTSVGTK--QDFPLGGTFRHEILANTERMVKAKSDVSISSAGTVTESSHQPQQSKLKSRSLEPLTSLAMWSDTLLEIELIKGERGLGFSILDYQDPMNKDETVIVIRSLVPGGVSQQDGRLIPGDRLMYVNDINLEHASLDEAVQALKGAPRGMVRIGVTKPLPLPEVSEQYDEDDSAHTESSSTPYDNTEVNSSDVNTERMSPSVNLPDLPQDDDTPIPPPLPTSPPPEDGDFDARTAERLLGTTSKGVISARFVSREEPIHEGDIVPPLPAALERTIVVRKDSDTLGIQ-VDIQDEGINGLVVRSVTSGGTLSRDGRIYPGDYLVAVNGEVMRKASHSQALDILRRT 1090
K G A+ + ++++ + GLGISL G ++ +I + PNGP +GV+K GDE+LEVNG L G H + AI+K L T K++ R + K FP+ + E K V+I G V E+ P ++ + L ++ IE E G G I D Q+ G ++Q G LI GD ++ VN + + D A LK G+V + V P E ++ D E + SS+++T R+SPS P+ G A T EEP + P P A E I + KD LG+ V D + +++ V G ++DGR+ PGD ++ N E R +HS+AL LR+T
Sbjct: 1748 KKYAAHGDAVCIVSVERG--HQGLGISLAGHKDRT-----KMAAFICGLNPNGPAFKSGVVKVGDEILEVNGVVLRGRCHLNASAIIKGLAGPTYKIILLRRSDALEELAVKPITQFPV--SLDDETPEEKFAHFKGLKIVTIKKPGYV-EAPPAPPSPQVAGQGL-----------GIMIIEGKHAEVGQGIFISDIQE----------------GSAAEQAG-LIVGDMILAVNKDTILGNNYDSAASLLKKT-EGVVTLVVCNPQKAKEDEKKAGGDKPKEPEKPRL-HLKPIAPSSEMSTARLSPS------------------PS------GKIHAGT--------------------EEPPADPATAPIKPGA-ESVIEINKDKMGLGLSIVGGSDTLLGAIIIHEVYPDGAAAKDGRLLPGDVILECNSEDFRNITHSKALAALRQT 2116 HSP 6 Score: 78.9518 bits (193), Expect = 1.409e-10 Identity = 73/249 (29.32%), Postives = 110/249 (44.18%), Query Frame = 0
Query: 1882 PVASPRRKAGDESSNPATCEITPGADTVIEISKDKDENG-----------KPMG--LGLSIVG--GSDTLLGAIFIHEVYEKGAAHKDGRLKPGDQILEVVHEDL-RNVSHSHALHALRQTPNRVRLMIHREDDEIYETL-----------------DVELNKKKDR-----------GLGLSIVGKKSGPGVFISEVVKGGAADSDGRLVQGDQIITVNGHDLTNSSQEDAAPILKMTQGKIAMRVRR 2086
P++S + A ES + IT A ++E K +E+ KP G LG S+V D IF+ E+ KG A +DGRL GDQIL + + L N+SH A L++ RV L++ R + L D+ LN + + GLG I+G +S GV + +++GG A+ DGRL GD I+ + G L +E A +L+ + + V R
Sbjct: 117 PLSSTEQIATPESKDVTLTPITT-ASYILEFQKAIEESAQGREIITIQLMKPEGGSLGFSVVSLRSEDKGELGIFVQEIQPKGIAGRDGRLHEGDQILAIDGQPLDTNISHQQAFSILQKARGRVELVVARTESPSPPPLNSPSRSPSPSNKSETGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGRS-TGVVVKTILRGGVAERDGRLQSGDHILQIGGVSLRGMGREQVAAVLRQAGSHVRLIVAR 363 HSP 7 Score: 75.0998 bits (183), Expect = 2.374e-9 Identity = 60/207 (28.99%), Postives = 93/207 (44.93%), Query Frame = 0
Query: 2003 ETLDVELNKKKDRGLGLSIVGKKSGP----GVFISEVVKGGAADSDGRLVQGDQIITVNGHDL-TNSSQEDAAPILKMTQGKIAMRVRRLRVGNRKQGVGGMHSRDSSIPSN-----VPGATGGTMKTVELKRGQHGLGFSIVGGFGSPHGDMPIYVKTVFDTGAAADHGGLKRGDQILSVNAKPLEGLSHHDAVNILKNCDGAVML 2199
E + ++L K + LG S+V +S G+F+ E+ G A DGRL +GDQI+ ++G L TN S + A IL+ +G++ + V R + S S S V ++ ++L GLGF I+GG + + VKT+ G A G L+ GD IL + L G+ +L+ V L
Sbjct: 158 EIITIQLMKPEGGSLGFSVVSLRSEDKGELGIFVQEIQPKGIAGRDGRLHEGDQILAIDGQPLDTNISHQQAFSILQKARGRVELVVARTESPSPPPLNSPSRSPSPSNKSETGSDMVLNTEWAQVEVIDLINDGSGLGFGIIGGRST-----GVVVKTILRGGVAERDGRLQSGDHILQIGGVSLRGMGREQVAAVLRQAGSHVRL 359 The following BLAST results are available for this feature:
BLAST of conserved hypothetical protein vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 16
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BLAST of conserved hypothetical protein vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of conserved hypothetical protein vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold871_size86487:43537..50369+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold871_size86487-snap-gene-0.25 ID=maker-scaffold871_size86487-snap-gene-0.25|Name=conserved hypothetical protein|organism=Tigriopus kingsejongensis|type=gene|length=6833bp|location=Sequence derived from alignment at scaffold871_size86487:43537..50369+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'conserved hypothetical protein' has the following synonyms
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