BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 174.481 bits (441), Expect = 1.090e-47
Identity = 145/543 (26.70%), Postives = 252/543 (46.41%), Query Frame = 0

Query:   80 ENKILKAQRPFEPVCLAQDQDSIIKHIFEKLDDLLADGQG-------GRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVT---QVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFN-HIVT--GVE-IMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSS----VTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGS-ISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPIT-TIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLME-HPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILR 601
            ENKIL +Q  F+   + +   +I  +  + +   +            G+ +    T  E P+   +        K A    + GL  GDVV   + N  D YIP+    LC  +VS++   +    + S++++TQ K ++C       ++       +I L+T              L++  +   Q+P      +    +ILW+SGTTG PKGIQ +  ++ NL+      +     T  F H+ G++ +   +I  +V   FH  +   VT  GVE I+    +     + +   H V +A       ++ SS    V  I P G+     + +DL+  F N + + N Y MTE G  I +     S G ++   EV I D ++ E LG N  GE+  + P   T+GY+NRP   +K F  DG+  +GD+ +Y++ G L++  R+K+++K   + VYP+E+ET++   +  I E  ++   E  +Q ++   VV   G  +   ++R + V+  +DD K +   +     LP+N +GK+LR
Sbjct:   11 ENKILTSQNDFDDSVVLEYNQNITSYFIDSMKKYMTKNSNKEFLVNCGKKYFPGETTSEGPESVSILDFIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKVKDL--SSIIINLDT---------QLHAILEIDMSTQTQIPEQIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLLFLSPKTDGNMLITCNFAHVSGFNYSCFWSINFDV-CTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTE-KIQTILGGIVVIKKGYRIDPEEIRQF-VNDRIDDYKHLEIIILTNEPLPRNPNGKLLR 539          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 164.466 bits (415), Expect = 1.074e-43
Identity = 129/531 (24.29%), Postives = 246/531 (46.33%), Query Frame = 0

Query:  114 LADGQGGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLC----YQGNLATIEQAITGQTLIALNTDGDESTDH----LSYERFLDMGEAEPFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLN-LVQPIVSPNPYS--------YTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSMGSLLNGREVKIVD--------PNTQELL------------GANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQDH 607
            + + +  R W+ D++     +++    V  L  K AS  TK G   GDV+  Y  N V+Y+I   G W CG IV   +  L    ++ Q+ +++ K+L+C    Y   L  +E     + LI +   G+E T      L  +   D G+  P ++          + ++S ++G  KGI H+++++++    P  + N +         +    F+HM G++    G+I+G        F+        +EA    K   +  +      L+ +    + DLSS+  +      L + +  ++  + P+ K +   YG+ E G +++ +      S+ + +   + D        PN   ++            G +Q GE+ VK+P   +GY+N+      +F  D ++ TGDLGYYD  G++YF +++ +LI++   +V P  LE+ L+    I + AV G P     ++  A VV  PG   +E ++   ++ + + D + IRG + FV ++P++ + K+LR +L ++
Sbjct:   69 IGEKEPDRIWIKDLS---IGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIII---GNECTAQDKVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSE----QNFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKE--ESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTN-LMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAEY 585          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 158.303 bits (399), Expect = 2.263e-42
Identity = 135/466 (28.97%), Postives = 208/466 (44.64%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLP---VTQVDQCTMILWSSGTTGLPKGIQHSHRNMLN--LVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPT-SMGSLLNGREVKIVDPNTQELLGANQIGE-MWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLME-HPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSH-LPKNAHGKILRKLLQ 605
            A +  + GL   DVV   + N  D Y+P+F   LC  ++S++  ++    + +++ DTQ +++ C       ++    G T+I ++TD          E  +  G      +P   V  +    +ILWSSGTTG PKG+Q +  + L+     P V  N     +  F H+ G+           ++ +F  F H  T      ++   K        H GV                             L E LK      K + N+YG TE G   +  T   S G ++   E  I D NT + LG N+IGE M+ K   T+ GY+NR   NEK F  DG+  TGD  +Y E G  +FH R+ ++IK + + VYP+ELE +L   H +I EV V    E   Q ++ A +V      ++   +R   ++  VD+ K I G +      LP+NA GK+LR  +Q
Sbjct:   87 AKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKVKNC--GCTIINMDTD---------IEEMIANGCTSKVIVPKQDVFSLKDVFVILWSSGTTGQPKGVQMTVVSFLDSMFATPKVKGN--MLLSANFGHISGF-----------ISSLFWTF-HFSTSTFYYISSDDVK--------HFGV---------------------------VKLFEVLK------KCVPNFYGSTESGGGFLVSTNQHSNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKK-QTVLGAIIVVKNDKKINVETIRKE-LNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQ 484          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 147.132 bits (370), Expect = 6.173e-38
Identity = 151/535 (28.22%), Postives = 245/535 (45.79%), Query Frame = 0

Query:  106 IFEKLDDL-----LADGQGGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAI---TG-QTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQCTMIL---WSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSY-----------TTTMFYHMGGYHLAVSGAI-FGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKP--RPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPN-CKAILNY---YGMTELGSISM-----GHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            I+E L+D      L  G  GR +  ++       V  LSQ      K  SA  +LG + GDV+   +PN  ++ I   G    G  ++  + T +   I  Q++++  K ++     L  I+Q+    +G Q +I +  + +  +D  S+       +   F    T +D    IL   +SSGTTG PKG+  +H N++N +  +  P+ +++               F+H+     ++S  + FG   +    F   +     + +  +YKP  M +     V    + P  +P L LSS+  ++   S+    L+E    KF N C + L +   YGMTE   ++      G    S G+L+     KI+D +T ++LG  + GE+ V  P    GY       +K    +G+  TGD+ YY++ G  Y  DRLK+LIK K  QV P ELE ++ ++P + +VA  G P+    EL  A VV+   S + +  +    V   V   K +   V FV  LPKN  GKILR+ L+
Sbjct:   66 IWENLEDYKEREALVCGMTGRTYTYEM-------VHSLSQ------KFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGME-ETPSDCKSFMEMAIFDDGSFFDQRET-IDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEM----FINSLVQYKPTHMLVVPPL-VSFMTSSPLIKPSL-LSSIKMLNGGASTFGPTLIE----KFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDK-IIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRSVMEQGIID--FVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 141.739 bits (356), Expect = 3.594e-36
Identity = 127/468 (27.14%), Postives = 211/468 (45.09%), Query Frame = 0

Query:  167 LPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDM----GEAEPFQLPVTQVDQCTMILWSSGTTGLPK---GIQHSHRNMLNLVQ-----------PIVSPNPYSY---TTTMFYHMGGYHLAVSGAI-FGNVNVVFHGFNHIV--TGVEIMEAAHKYKPNLMFIWTHHGVELAMAKP--RPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPE-IAEVAVYGTPEPAVQELISACVVK---TPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            L N   ++IP+ G W  G+ VSL   +     I+ Q+      ++L  + NL  I        ++   T       ++  +R + +    G     ++ +   ++   I    GTT L     G+ +S  N+LN +             I +  P S      T F+H GG+   +   +      ++    N  V  +   +  A    KPN++       V++  A        DLSSV  I P+G S++TN  +  + +FP+ K I   YG TE  SIS+G +   +G L    +++I++PN  E LG N +G++ VK       YI   G +E +   DGF+  GD+GYYD AG ++F  R KDL+K  N+ VYP E+E+ +      + ++ V+G  +    E +S  +V      G +L     R +  +      K ++G V+  S +P+NA+GKI R  L
Sbjct:  101 LNNTRFFFIPVVGTWFLGSSVSLFQPSSSRETIERQL------MILKEELNLEEI--------VVITETFAIPKISNIGVDRIISLTPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQHLGRLFPKYQLRILNPNG-EPLGPNSVGDIVVKPGYGMRRYI---GDHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAYK-FPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 101.293 bits (251), Expect = 2.215e-22
Identity = 71/222 (31.98%), Postives = 108/222 (48.65%), Query Frame = 0

Query:  388 GSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTP--------TSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILR 601
            GS+      +D + K  N K+++N YG TE G +S    P         ++G  L   E KIVD N + +L  N  GE+WV+TP    GY      ++K   PD +Y +GDLG   E G      RL D++      +YP E+E VL++HP I EV + G P+  + E + A +    G+   E D++       +   K+ + P+ FV   PK   GK+ +
Sbjct:  319 GSNCLVEACKDFE-KHLNMKSLMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGA--KEFDLKEH-CKGKISHFKIPKYPM-FVKDFPKTLSGKVKK 534          

HSP 2 Score: 100.523 bits (249), Expect = 3.061e-22
Identity = 74/249 (29.72%), Postives = 117/249 (46.99%), Query Frame = 0

Query:  365 VELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTP--------TSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADV----RAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILR 601
            V +  A+ +  +D+SSV      GS+    + ED + K  N K+++N YG TE G  +   +P         ++G  L   E KIVD N + +L  N  GE+WV+TP    GY      ++K+  PD +Y +GDL    E G      RL D++      +YP E+E  L++HP I EV + G P+  + E + A +    G+   E D+    +  + H  +    +      FV   PK   GK+ +
Sbjct:  667 VAVLEAQKKLKVDVSSVELGITGGSNCLVEVCEDFE-KHLNMKSLMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGA--KEFDLKEHCKGKIAHFKIPKYSM------FVKDFPKTLSGKVKK 905          

HSP 3 Score: 95.1301 bits (235), Expect = 1.810e-20
Identity = 65/203 (32.02%), Postives = 97/203 (47.78%), Query Frame = 0

Query:  407 KAILNYYGMTELGSISMGHTP--------TSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILR 601
            K + N YG TE G +S    P         ++G  L   E KIVD N + +L  N  GE+WV+TP    GY      ++K   PD +Y +GDLG   E G      RL D++      +YP E+E  L++HP I EV + G P+  + E + A +    G+   E D++       +   K+ + P+ FV   PK   GK+ +
Sbjct:  109 KKLRNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGA--KEFDLKEH-CKGKIAHFKIPKYPM-FVKDFPKTLSGKVKK 306          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000006577 (supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-1 gene:EMLSAG00000006577 transcript:EMLSAT00000006577 description:"maker-LSalAtl2s360-snap-gene-1.18")

HSP 1 Score: 68.1662 bits (165), Expect = 2.937e-12
Identity = 98/472 (20.76%), Postives = 182/472 (38.56%), Query Frame = 0

Query:  147 KAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLL----CYQGNLATIEQAITGQTLIALNTDGDESTD-HLSYERFLDMGEAEPFQLPV---TQVDQC-------------------TMILWSSGTTGLPKGIQHSHRNMLNLVQPIVS--------PNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDL----DLSSVTAISPLGSSLDTNLLEDLKPKFPNCK-AILNYYGMTELGSISM----GHTPTSMGSL---LNGREVKIVDPNTQELLGANQIGEMWVKTP---ITTIGYINRPGLNEKSFAP-DGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVR 567
            K ++     G++ GD V  Y+P  ++  + +      GA+ S+        ++ S+I D   KVL+     Y+GN       +  + +   +++ +   D H+       +    P + P    T+ D                       +L++SG+TG PKG+ H+    +                 + Y  T  + +  G  ++     + G+ +V+F G     T     E   KY  +  +  T      A+ K   D     DLSS+  +  +G  ++              + +I++ Y  TE G   M    G TPT  GS      G    I+D + + L G  + G +    P   +    Y N     +  F+   G+Y +GD    D+ G ++   R+ D++    + +   ++E+ L+EH  +AE AV  TP P   E +   +      +L++  V+
Sbjct:   69 KFSNVLKSKGVQKGDRVAIYMPMIIELVVAMLACARIGAVHSIVFGGYSSDSLASRIVDGGAKVLISADGVYRGNKLINLLDVAYKAMEETSSNYNHDIDIHILVSHIPRLNSGSPLETPSRWKTERDHWWHEVMEPASDVEWMNSEDPLFLLYTSGSTGKPKGVLHTTAGYMIYAATTFKYVFDYQDESDVYWCTADIGWITGHTYVTYGPLLNGSKSVLFEGTPFYPTNDRFWEIIQKYSVSKFY--TAPTAIRALMKFGEDFVSKYDLSSLKVLGTVGEPINPEAWFWYHKNVGGSQCSIVDTYWQTETGGHIMTPLPGCTPTKPGSACFPFFGVSPVILDESGKVLSGEAE-GYIVFNKPWPGMMRSVYGNHERFEKTYFSRFPGYYMSGDGAKRDKDGYIWITGRIDDMLNCSGHLMSTAQVESALVEHRHVAEAAVVSTPHPIKGECLYCFITPMADISLNDKMVK 537          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 64.6994 bits (156), Expect = 3.667e-11
Identity = 92/373 (24.66%), Postives = 150/373 (40.21%), Query Frame = 0

Query:  272 LWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHL----AVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWT---HHGVELAMAKPRP-------------DLDLSSVTAISPLGSSLDT---NLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSMGSLLN---------GREVKIVDPNTQELLGANQIGEMWVKTPITTIG---YINRPGLNEKSF-APDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKT---PGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            L++SG+TG PKG+ HS    L            S+T  + +    + +    A  G I G+  +V+    +  T V + E+   Y P+    W      G+      P               + D SS+  +  +G  ++    N   DL  +  N   I++ +  TE G I +   P+ + + +          G    ++D    E+   N+ G + +KTP   +    Y +     E  F A  G+Y TGD  Y  + G +    R+ D+I    +++   E+E VL +H  I E AV G P     E I A VV      G    E  ++  V   D   G  +    +F   LPK   GKI+R++L+
Sbjct:  207 LYTSGSTGAPKGLVHSTAGYLLYT---------SFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSV-LFESTPTY-PDPSRYWKLVEELGITQFYTSPTAIRQLMKYEDSYVLNSDKSSLRTLGSVGEPINIEAWNWYNDLVGR--NRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNEIKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLV--KDNISGFAVPEKFQFCEDLPKTRSGKIMRRILR 564          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 63.1586 bits (152), Expect = 1.534e-10
Identity = 88/374 (23.53%), Postives = 162/374 (43.32%), Query Frame = 0

Query:  270 MILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFN-----HIVTGVEIMEAAHKYKPNLMFIWTHHGVE--LAMAKPRPDLDLSSVTAISPLGSSL-------DTNLLEDLKPKFPNCKA--ILNYYGMTELGSISMGHT-------PTSMGSLLNGREVKIVDPNTQELLGANQI---------GEMWVKTPITTIGYINRPGLNEKSFAPDG-FYKTGDLGYYDEAGLLYFHDRLK-DLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKL--IRGPVKF--VSHLPKNAHGKILRKLLQ 605
            +IL++SGT+G PKG+  +HRN+   V  ++    ++    + + +  YHL       G +N +    +      ++ G +  +       + M I    GV    ++     +LD  + T I  +  S          +L E L  K+       +L  YGMTE+G + +G++       P  +G+     E +I+      L  +N +         GE+ ++ P     Y       ++SF P+G ++ TGD    +  G+     R   D+IK    ++  +E+E   + +P+I+E+AV G  +    E+++A +V   G  L+   +R +         KL     P +F  V+ + KN  GKI +K L+
Sbjct:  151 LILYTSGTSGSPKGVWITHRNIEAQVSGMIKFWAWTSDDHILHVLPLYHL------HGLINCLLVPLSIGARVKMLPGFDAKKVWSYLTNSSMNISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRKWQTISGHILLERYGMTEIG-MGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYK------KLPPYSVPRQFMLVNSIDKNHMGKINKKELK 511          

BLAST of luciferin 4-monooxygenase vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 56.9954 bits (136), Expect = 1.442e-8
Identity = 53/210 (25.24%), Postives = 97/210 (46.19%), Query Frame = 0

Query:  136 QHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGN-LATIEQAI----TGQTLIALNTDGDESTDHLS-YERFLDMGEAEPF-----QLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFH 334
            Q+ S +K      A AF KLGL+    V  Y  NC +++I   GA   G + +    T      +  + +++ ++L+      L TI   +    T + ++ +  DG    ++L  ++  L++G  E       +L    V+QC  ++++SGTTG PKG+  SH  +  + + IV    + +TT  F  +    L+   A F ++ V  +
Sbjct:   79 QYYSDIKL----TAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVK--KHDWTTAKFIVVSYLPLSHIAASFLDIYVALY 282          

HSP 2 Score: 51.6026 bits (122), Expect = 5.819e-7
Identity = 43/188 (22.87%), Postives = 84/188 (44.68%), Query Frame = 0

Query:  148 AASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQT-----LIALNTDGDESTDHLS--YERFLDMGEAE-----PFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSG 323
            AA AF KLGL+    V  Y  NC ++ I   GA   G   +    +      +  + +++ ++L+     +    Q I         ++ +  DG  + D LS  +   +++G+ E       +L     +QC  I+++SGTTG PKG+  SH  +    + +V  + ++   ++  ++   H+A + 
Sbjct:  705 AAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVLQKVQPILENIKHLLKVVVIEEDGFIA-DKLSILWRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANA 891          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 206.453 bits (524), Expect = 6.277e-58
Identity = 158/482 (32.78%), Postives = 234/482 (48.55%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQ-VDQ--CTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVS-------PNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFN-HIVTGVEIMEAAHKYKPNLM------FIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSM---------GSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            A   +++G+  G VV    PN + + +        GAI++ T+       I  QI+D+ P +       L  I  A     ++ ++ +  +S   +   R ++M + EP    V + VDQ     +L+SSGTTG+ KG+  SHRN++ +VQ IV+          +  T  MF+  G    A     +G+  +V   F  H     E+M A  KY+   +       +   +G +   AK     DLSS+  +   G+ L   + E    K+P  K IL  YG+TE  S  +G +  ++         G L    E +IVDP T ++LG  Q GE+W+K P    GY +       +   +G+ +TGDL Y DE G ++  DRLK+LIKYK  QV P ELE +L+ HPEI + AV   P+  V +   A VV+  GS+LSE  +  +V    V   K IR  V FVS +PKN  GKILRK L
Sbjct:   71 ADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDV--RRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMH-----EMMSAIGKYQATSLPLVPPILVAMVNGADQIKAK----YDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVK-ILQGYGLTE--STGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAK-QVAPYKRIR-KVAFVSSIPKNPSGKILRKDL 536          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 189.889 bits (481), Expect = 8.041e-52
Identity = 148/492 (30.08%), Postives = 245/492 (49.80%), Query Frame = 0

Query:  138 LSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDG-DESTDHLSYERFLDMGEAEPFQLPVTQV--DQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIV---SPNPYSYTTTM------FYHMGGYHLAVSGAI-FGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNY-YGMTELG-----SISMGHTP-----TSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
             S+V+ ++ K A+   KLGL+ G VV   LPNC+++     GA + GAIV+  +   K   I  Q +    ++++     +  +    +   L+    D   E   H+S      + EA+  Q P  ++  D    + +SSGTTGLPKG+  +H+ +++ V   V   +PN Y ++  +       +H+   +  +  A+  G   ++   FN + T +E+++   KYK  +  I     +++  +      D+SSV  I    + L   L + L+ +FP  KAI    YGMTE G     +++    P      S G+++   ++KI+D  T E L  NQ GE+ ++ P    GYIN P     +   +G+  TGD+ Y D+   ++  DR+K++IKYK  QV P ELE +L+ HP IA+ AV         E+  A VVK+  S +SE +++ +V    +   K+ R  V FV  +PK+  GKILRK L+
Sbjct:   55 FSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQHISV-----LTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFN-LTTCLELIQ---KYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFP--KAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVVKS--SEISEQEIKEFVAKQVIFYKKIHR--VYFVDAIPKSPSGKILRKDLR 531          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|122247566|sp|Q10S72.1|4CLL4_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 4)

HSP 1 Score: 189.889 bits (481), Expect = 8.724e-52
Identity = 155/476 (32.56%), Postives = 236/476 (49.58%), Query Frame = 0

Query:  155 LGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPA-IQSQIEDTQPKVLLCYQGNLATIEQAITGQTLI--ALNTDGDESTDHLSYERFLDMGEAEP----FQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVS---------PNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAM-AKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSM---------GSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            + L  G V     PN V + +    A   GA+++ T++ L  PA I  Q+ D +P +    +  L  + +A   + ++  +    GD S+D        ++    P     +  VTQ D  T+ L+SSGTTG  KG+  +HR+++++VQ I++            +  T  MF+  G    A      G   VV   +       E++ + + Y   + ++     + +AM A P+P L L  +  +   G+ L   L+E  + K+P  + IL  YG+TE  S ++G +  S          G L    E KIVDP++ E L  N+ GE+W++ P    GY       + +  PDG+ KTGDL Y DE G L+  DRLK+LIKYK  QV P ELE +L+ HPE+ +VAV   P+  V +   A +V+  GS LSE +V  +V    V   K +R  V FV+ +PKNA GKILRK L
Sbjct:   84 VSLRKGHVALILSPNSVHFPVAALAAMSLGAVLT-TANPLNTPAEIAKQVADARPVLAFTTRELLPKLPRAHDLRVVLLESARLPGD-SSDPRIVATIEEISATTPDPARRKDRVTQDDPATL-LYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTETFLCTVPMFHVYGLVAFATGLLGCGATVVVLSKYEL----PEMLRSINAY--GVTYLPLVPPILVAMVAHPKP-LPLGQMRKVLSGGAPLGKELIEGFREKYPQVE-ILQGYGLTE--STAIGASTDSAEESRRYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTPDGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPDREVGQFPMAYIVRKKGSNLSEREVMEFVAK-QVAPYKKVRK-VAFVTDIPKNASGKILRKDL 544          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|209572803|sp|Q0DV32.2|4CLL1_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 1)

HSP 1 Score: 189.119 bits (479), Expect = 2.117e-51
Identity = 148/479 (30.90%), Postives = 230/479 (48.02%), Query Frame = 0

Query:  148 AASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGD------ESTDHLSYERFLDMGEAEPFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVS--------PNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE-LGSISMGHTPT-------SMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            A +  ++ G+  GD V    PNCV Y +  F     GA+ +  +       I  Q+ D + K+++     +  I        L+  + +        ++T  L       + EA+  + P+ Q D   + L+SSGTTG  KG+  +HRN +   + + S        PN +     MF+  G   +  +    GN  +    F+       +MEA  +++   +F      + LA        DLSS+  I    + L  +++E +  KFP+ + I+  YGMTE  G IS+ +          S G+L++G E KIVD  T + L  NQ+GE+ V+ P    GY N     E +    G+  TGDLGY+D  G L+  DRLK+LIKYK  Q+ P ELE +L+ HPEI +  V   P+    E+  A VV++P S+L+E DV+ ++        +L R  V FV  +PK+A GKILR+ L
Sbjct:   74 AVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEADYRRPPIKQSDTAAL-LYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHIFGLSVITYAQLHRGNAIIAMSRFDI----NSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSE-IVQGYGMTETCGIISLEYPEKGQAREFGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTIK-QGWLHTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAGEVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKR--VTFVGSVPKSASGKILRRQL 543          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|158564047|sp|Q84P23.2|4CLL9_ARATH (RecName: Full=4-coumarate--CoA ligase-like 9; AltName: Full=4-coumarate--CoA ligase isoform 4; Short=At4CL4)

HSP 1 Score: 189.119 bits (479), Expect = 2.449e-51
Identity = 148/484 (30.58%), Postives = 231/484 (47.73%), Query Frame = 0

Query:  138 LSQVKPLAFKAASAFTKLGLEAGDVVQFYL-PNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNML--------NLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIM-EAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTEL-GSISMGHTPTSM------GSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            L +V+ LA      F  L   A   V F L P+ +D  +        G +VS  +       +  Q+E ++P +       +  ++      + + L T   +ST+ LS+    D     PFQ+ V Q D    IL+SSGTTG  KG+  +HRN++          +Q  V+ +     +   +H+ G+ + +     G   V+   F      +E M +A  KYK   M +     V L  ++     DL S+ ++   G+ L  ++ E  K KFP+   I+  YG+TE  G  +    P  M      G +    E KIVDP+T E L   + GE+W++ P+   GY+     + ++   +G+ KTGDL Y+D    LY  DRLK+LIKYK  QV PVELE +L  +P++ + AV   P+    E+  A +V+ PGS L+EA +  +V        K+ R  V F++ +PKN  GKILR+ L
Sbjct:   84 LRRVRSLAVSLRERFPSL---ASRNVAFILSPSSLDIPVLYLALMSIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQS-----SSLPLGTVLMDSTEFLSWLNRSDSSSVNPFQVQVNQSDPAA-ILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFE-----LEAMFKAVEKYKVTGMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVD-IVQGYGLTESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRR--VAFINAIPKNPAGKILRREL 550          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 184.111 bits (466), Expect = 1.066e-49
Identity = 140/487 (28.75%), Postives = 231/487 (47.43%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNL---ATIEQAITG-QTLIALNTDGDESTDHLSYE---RFLDMGEAEPFQ------LPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQ----PI----VSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISM-----GHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKI----LRKLLQ 605
              A    GL     +     NC +++IP+      G  V+ T+    +  +   +  ++P ++   +  L    T+++ +T  +T++ L    D   D+  Y+    F+       FQ      + V + +Q  +I+ SSG+TGLPKG+Q +H N +        PI    VSP     T   F+H  G    +   I G   V+   F+        ++    YK   + +       L  ++     DLS++  I+  G+ L   + E +  +F N   +   YG+TE  S  +        P + G ++   + K++D +T++ LG N+ GE+ VK P+   GY+N P   ++    +G+  TGD+GYYDE    +  DRLK LIKYK  QV P ELE+VL++HP I +  V G P+P   EL  A VV   G  ++E +V  +V  + V + K +RG V+FV  +PK   GKI    +R++L+
Sbjct:   66 GKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVIL----DSKVDYRGYQCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEET----FLKTLQDYKCTSVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVA-SQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREILK 542          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|112800|sp|P14912.1|4CL1_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 184.111 bits (466), Expect = 1.100e-49
Identity = 149/489 (30.47%), Postives = 248/489 (50.72%), Query Frame = 0

Query:  139 SQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIV---SPNPYSYTTTM------FYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLM-FIWTHHGVELAMAK-PRPD-LDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSI-----SMGHTPTSM-----GSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            SQV+ L+ K AS   KLG++ GD +   LPN  +Y+    GA   GAI ++ +       +  Q++ +Q K+++     +  ++     + +  +  D D   D L + + ++  E+E  ++ V   D    + +SSGTTGLPKG+  +H+ ++  V   V   +PN Y ++  +       +H+   +  +   +   V ++      IV  +E+++   KYK  +  F+     + LA+AK P  D  DLSSV  +    + L   L + ++ KFPN K +   YGMTE G +     +    P  +     G+++   E+KIVDP T   L  NQ GE+ ++      GY+N P     +   +G+  TGD+G+ D+   L+  DRLK++IKYK  QV P ELE +L+ HP I++ AV    +    E+  A VV+T G T +E +++ +V    V   ++ R  V FV  +PK+  GKILRK L+
Sbjct:   57 SQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLIITQACYVDKVKDYAAEKNIQIICID-DAPQDCLHFSKLMEADESEMPEV-VINSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQ---KYKVTIGPFVPP---IVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAK-LGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFR--VFFVDAIPKSPSGKILRKDLR 534          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|75311763|sp|Q9M0X9.1|4CLL7_ARATH (RecName: Full=4-coumarate--CoA ligase-like 7; AltName: Full=4-coumarate--CoA ligase isoform 6; Short=At4CL6)

HSP 1 Score: 183.726 bits (465), Expect = 1.501e-49
Identity = 142/492 (28.86%), Postives = 229/492 (46.54%), Query Frame = 0

Query:  138 LSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIA----LNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQC--TMILWSSGTTGLPKGIQHSHRN------MLNLVQPIVSP--NPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNH--IVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPR--PDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPT-------SMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
             SQ+K    + A  F +LG+   DVV  + PN   + +        G + +  +    V  +  QI+D+ PK+++        I+       L+     +      ++  LS++  +++ E    + P  ++ Q     +L+SSGTTG  KG++ +H N      M+ + Q ++      +     MF+  G   +  S    GN  V    F    ++  +E     H        +W    V LA++K       DLSS+  I    + L  +L+E+     PN   ++  YGMTE   I     P        S G L  G E +IV   T +    NQ GE+WV+ P    GY+N P   +++     +  TGDLGY++E G LY  DR+K+LIKYK  QV P ELE +L+ HP+I +  V   P+    E+  A VV++P S+++E D++ ++        +L R  V F+S +PK+A GKILR+ L
Sbjct:   57 FSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS-EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTH--------LWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNV-LLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRR--VSFISLVPKSAAGKILRREL 536          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 183.726 bits (465), Expect = 1.726e-49
Identity = 135/481 (28.07%), Postives = 231/481 (48.02%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNL---ATIEQAITG-QTLIALNTDGDESTDHLSYERFLDMGEAEPFQ------LPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQ----PI----VSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISM-----GHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQD 606
              A    GL     +     NC +++IP+      G  V+ T+    +  +   +  ++P ++   +  L    T+++ +T  +T++ L++  D    + S + F+     + F+      + V + +Q  +I+ SSG+TGLPKG+Q +H N +        PI    VSP     T   F+H  G    +     G   V+   F+        ++    YK + + +       L  ++     DLS++  I+  G+ L   + E +  +F N   +   YG+TE  S  +        P + G ++   + K++D +T++ LG N+ GE+ VK P+   GY++ P    +    +G+  TGD+GYYDE    +  DRLK LIKYK  QV P ELE+VL++HP I +  V G P+P   EL  A VV   G +++E +V  +V  + V + K +RG V+FV  +PK   GKI  K +++
Sbjct:   66 GEALKNYGLVVDGRIALCSENCEEFFIPVLAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTAIKTIVILDSKVD-YRGYQSMDNFIKKNTPQGFKGSSFKTVEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRFSHARDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYLTCGFRIVMLTKFDEET----FLKTLQDYKCSSVILVPTLFAILNRSELLDKYDLSNLVEIASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVA-SQVSNAKRLRGGVRFVDEVPKGLTGKIDGKAIRE 539          

BLAST of luciferin 4-monooxygenase vs. SwissProt
Match: gi|158517764|sp|P0C5B6.1|4CLL4_ARATH (RecName: Full=4-coumarate--CoA ligase-like 4)

HSP 1 Score: 182.185 bits (461), Expect = 5.011e-49
Identity = 155/491 (31.57%), Postives = 227/491 (46.23%), Query Frame = 0

Query:  152 FTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSL-----TSSTLKVPA-IQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFL----DMGEAEPFQLPV---TQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPN-----PYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFN-HIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKP------------RPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE-------LGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            + ++G+  GDVV    PN +  +IP+    +C +++SL     T++TL     I  QI D+ P ++   +     +  AI+      + TD +   +  S  R +    +M + EP    V      D   M+L+SSGTTG  KG+  SHRN+   V   +S N      +  T  MF+  G    A+     G+  V+   F  H     ++M+A  K          H    LA+A P            +   DLSS+  +   G+ L   + E    K+P    IL  Y +TE         S        + G+L +  E +IVDPNT   +G NQ GE+W+K P  + GY        ++   +G+ KTGDL Y DE G L+  DRLK+LIKYK  QV P ELE +L+ HP+I + AV   P+    +   A VV+   S LSE  V  + +   V   K IR  V F++ +PK A GK LRK L
Sbjct:   81 YHEVGIRRGDVVLILSPNSI--FIPV----VCLSVMSLGAVFTTANTLNTSGEISKQIADSNPTLVFTTRQLAPKLPVAIS-----VVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLH-----DMMDAVEK----------HRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVD-ILQGYALTESNGGGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDF-ISKQVAPYKKIR-KVSFINSIPKTASGKTLRKDL 542          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 246.514 bits (628), Expect = 2.227e-70
Identity = 168/549 (30.60%), Postives = 266/549 (48.45%), Query Frame = 0

Query:   76 MSRVENKILKAQRPFEPVCLAQDQDSIIKHIFEKLDDLLADGQ--GGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQC--------------TMILWSSGTTGLPKGIQHSHRNM-LNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVE-IMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQE-LISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            MS + +K+L ++   EP  L   Q+ II   F+ LD + A+ +  G R W+ D T     ++ H   +   + K AS  T+LG + GD +      C+D+Y P+FGAWLCG +VS+    L    ++SQI+DT  K+++C   + +   +A   Q+L++     +     L   + +  G +    L   Q DQC               +I W+SGTTG PKG+  S   +   + Q  +        +  F+H G + L++ G I    ++ F    H  T VE I     KYKP       +  V+LA +      DLSSV  I P G+ +  +    +K KFPN K +  +YG TE+G +S   T  +MG+L  G +  I D  T E LG  ++GE+  KTP   I Y+NR   + +    +GF   GDLGYYDE G+L++ +R+K++IK  N  + P E E  L   P + E  V+ +         I   V     S L+   +   + + DV+  + I G +  ++++P N  GK LR+ ++
Sbjct:    1 MSEIRDKVLYSK---EPYTLDPGQERII---FKLLDLIQAELRETGDRDWILDCTTENSVRISH---ILGRSIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPIFGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEA--NQSLLSAEQASNILVLDLEPWQPIPEGTSSFRSLYEDQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQSKLPEKTIGLASNTFFHAGSFLLSLDGGIRNKFSIHFIQPKH--TTVESIFRFIEKYKPMFFMCGANDFVQLA-SSDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGGVSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVWQSMNKETDNYYIHGAVTLQASSGLNPEKILE-LANKDVEVKRRIDGTIHIINNMPHNPQGKKLRRTVR 534          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|768409299|ref|XP_011552081.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 244.588 bits (623), Expect = 1.116e-69
Identity = 159/476 (33.40%), Postives = 248/476 (52.10%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQCT--MILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTM-----FYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGV----EIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE------LGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQDH 607
            A +  ++G+++GD V     N +D++  L GA L GA VS  +       I+  +   +PK++ C        E +I    LI +    ++  +   ++  +         LPV  V Q    MIL+SSGTTGLPKG+Q +H N +       +P      T +     F+ MG         ++G V  + +GF  +  G       +E   +YKP    +     V LA ++   D D+SSV  +    +S+D   + ++K +FPN   +   YGMTE        ++  G  P S+G++  G  VK+VD  T+E LG N+ GE+ VK+P    GYI +     ++F  +GFY+TGD+GYYDE G  +  DRLK+LIKYK  QV P ++E  L++HP + + AV G P+ A  E+  A VV+ PGS +S  +++ +V  + V + K +RG V+FV  +PKN  GKILR++L+D 
Sbjct:   61 AVSLARMGVKSGDSVAICTENRLDFWPTLLGAMLSGASVSPINPMYTRDEIKHTLGICKPKIIFCSPSAHKAHETSIKSFNLIRIAVYQEKIPNTYFFKDLISNKVRREDFLPVEVVGQTATLMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMG---------LWGTVGNLCNGFTVVYMGKFNEQRYLETIQRYKPGYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVARDTVDGGVKPGSVGTMPAGFVVKVVDLETREALGPNKPGEICVKSPTVMKGYIGKN--RTEAFDDEGFYRTGDIGYYDEDGFFFIVDRLKELIKYKGYQVPPAQIEAELLKHPAVRDAAVVGRPDKAAGEVPVAFVVRQPGSEVSGEEIQEYVA-SKVSNPKRLRGGVRFVDEIPKNPSGKILRRILRDR 524          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|768429780|ref|XP_011556085.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 244.202 bits (622), Expect = 1.201e-69
Identity = 168/515 (32.62%), Postives = 258/515 (50.10%), Query Frame = 0

Query:  110 LDDLLADGQGGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQCT--MILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTM-----FYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTG----VEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE------LGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQDH 607
            LD LL    G R    D    E      ++Q        A +  ++G+++GD V     N +D++  L GA L GA VS  +       I+  +   +PK++ C        E +I    LI +    ++  +   ++  +         LPV  V Q    MIL+SSGTTGLPKG+Q +H N +       +P      T +     F+ MG         ++G V  + +GF  +  G       +E   +YKP    +     V LA ++   D D+SSV  +    +S+D   + ++K +FPN   +   YGMTE        ++  G  P S+G++  G  VK+VD  T E LG N+ GE+ VK+P    GYI +     ++F  +GFY+TGD+GYYDE G  +  DRLK+LIKYK  QV P ++E  L++HP + + AV G P+ A  E+  A VV+ PGS +S  +++ +V  + V + K +RG V+FV  +PKN  GKILR++L+D 
Sbjct:   27 LDTLLK--HGDRTAFIDAITHETISYGEIAQN---GMNLAVSLARMGVKSGDSVAICTENRLDFWPTLLGAMLSGASVSPINPMYSRDEIKHTLGICKPKIIFCSPAAHKAHETSIKSFNLIRIAVYQEKIPNTYFFKDLISNKVRREDFLPVEVVGQTATLMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMG---------LWGTVGNLCNGFTVVYMGKFNERRYLETIQRYKPGYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVCRDTVDGGVKPGSVGTMPAGFVVKVVDLETHEALGPNKPGEICVKSPTVMKGYIGKN--RTEAFDNEGFYRTGDIGYYDEDGFFFIVDRLKELIKYKGYQVPPAQIEAELLKHPAVRDAAVVGRPDKAAGEVPVAFVVRQPGSEVSGEEIQEYVA-SKVSNPKRLRGGVRFVDEIPKNPSGKILRRILRDR 524          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325319021|ref|XP_023341279.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 232.261 bits (591), Expect = 5.517e-65
Identity = 156/510 (30.59%), Postives = 261/510 (51.18%), Query Frame = 0

Query:  119 GGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLC---YQGNLATIEQAITGQTL-----IALNTDGDESTDHLSYERFL----DMGEAEPFQ-LPVTQVDQCTMILWSSGTTGLPKGIQHSHRNM--LNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELG--SISMGHTPTS--MGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPE--PAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQDH 607
            G R W+ D    E  +   +S+++P++ + A A   LG   GDV+Q    +C+D+Y P FGAWLCG  VSL    L   AI+ QI+DT+ K+++C   Y      I+Q +  + +     +    DG+   D  S++ FL    D+   E  + +P    D  TM+LWSSG+TG PKGI HSHR +  ++L +P  SP     +  MF H+GG+ L +S A+FG     F       +G    + A K++P  ++I     ++++ AK   D +LS V  I+PLG ++     E +     +   I+  YG TE+   +  +   P    +G+L +G E+ I D  T E LG  + G+M V+T    + Y+NR   N + F  DGF   GD+GYYDE G ++F  R+++++K  N    P E+E ++    +I E  V+G  +      ++    V++      +E  +  ++  +++   + I G +  +  +P N  GK LR+ ++++
Sbjct:   40 GDRDWIVDT---ETGQSHTISKIEPVSRRVAGALHNLGFGPGDVLQTGYSSCLDFYWPAFGAWLCGGAVSLADPNLSSTAIKQQIKDTKAKIIVCSKQYLDKYCAIKQELREEDVHFNLSVLDEEDGNLPADVKSFQSFLNFDGDISHNEKLEYIP----DSVTMVLWSSGSTGNPKGILHSHRTLQTISLAKP-SSPKSILVSAIMF-HIGGFTLNLSIALFGGTTSYFIK-EKCFSGENWFKIAEKFQPEHVYIGVSQFIQVSKAK-NVDRNLSGVKMITPLGGAVSPECCEKVLSMVGDQAKIIEMYGSTEIVFLAFKLNKHPKFGFLGTLGSGAEMYIQDIKTGEKLGPRKEGKMMVRTRSMMMNYLNREEENREFFNSDGFGYVGDVGYYDEEGNIFFCYRMREVLKVDNYWFGPEEIENLMEALDDINEACVWGEYDMNTGNDQVSLGIVLQANKEEWTETRINEYIT-SNLPITRRITGKIYLLDRIPHNPQGKKLRREMKNY 537          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|1325273277|ref|XP_023321279.1| (4-coumarate--CoA ligase-like 7 [Eurytemora affinis])

HSP 1 Score: 229.565 bits (584), Expect = 4.509e-64
Identity = 157/518 (30.31%), Postives = 260/518 (50.19%), Query Frame = 0

Query:  106 IFEKLDDLLA--DGQGGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQGNLATIEQAITGQTL----IALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQ-------VDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSIS---MGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAV--QELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            I+  LDD+    D  G R WM D  +    K   LS + P++ + ASAF+KLG +AGDV+Q     C+D+Y P+FGAW+CG +VSL   +L    I+ QI D + K+++C   +     + +   T+    +    D  E+   +    F ++ E +    P  +       +D+  ++LWS+GTTG PKGI H H+ + N+       N  S  T + YHM G+   +S  I G   + +     + +G   + A  KY+P            +A AK +P  +L  +  ++PLG SL    +E +        + L  YG TE+G I          ++G L  G ++   DP T   LG +Q G+  VKT    +GY+ +   N++S+  DGF  +GD+GYYDE G +YF  R+KD++K +N    P E+E +L+  PEI +  V+G  +      ++    + +   ++ +EADV++ ++   +   + I G V  ++ +P +  GK LR+ +Q
Sbjct:   24 IYRVLDDVHQELDETGDRDWMVDTEDGGSTK---LSSIYPISIRIASAFSKLGFKAGDVIQTGYSTCLDFYWPIFGAWMCGGVVSLGDPSLNPDLIRYQILDLKSKIIVCSSEHALKYSKIVASLTVEEKPMLFVLDVLENKVPIGCRSFTELFEDDGLDAPSYESLPPYNPLDRL-VVLWSAGTTGNPKGIVHCHQTLQNVAYLKNICNK-SLITNVGYHMAGFLHHLSSGILGR-TISYFVKERLFSGDTYLNALEKYQPEYFVCSVSSFTSIANAK-QPFRNLPKLKIVTPLGGSLSPGTVERVMKILGEHVSFLKIYGGTEMGLIGKCVYSKDYDNIGQLGPGVQLYCADPVTGSKLGPHQEGKFMVKTRSMMLGYLKKSD-NDESYDSDGFAYSGDMGYYDEHGNIYFKCRMKDVLKVENFWFGPGEIENILLNEPEILDAVVWGVYDNTTGSDKVHLGVIFENTENSWTEADVKS-LIAKKLPRTRHITGQVHILTKIPYSVQGKKLRREMQ 532          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|358031578|ref|NP_001239600.1| (luciferin 4-monooxygenase [Bombyx mori] >gi|355525889|gb|AET05796.1| luciferin 4-monooxygenase [Bombyx mori])

HSP 1 Score: 221.09 bits (562), Expect = 7.789e-61
Identity = 155/473 (32.77%), Postives = 241/473 (50.95%), Query Frame = 0

Query:  149 ASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLL----CYQGNLATIEQAITGQTLIALNTDGDESTDHLSYE----RFLDMGEAEPFQLPVTQVDQCTMILWSSGTTGLPKGIQHSHRNML--NLVQPIVSPNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTEL-GSISMGHTPT----SMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQD 606
            ASA  +LG+  GD+V     N  +Y          GA V+  +S           + ++PK +      Y+    T+++    Q         D+  D +S+     ++    +  P     T  D  ++IL+SSGTTGLPKG++ +HRN+L   L +P    +    T   + +  G  L +S        +  + F   V     ++   KYK  ++ +     V L  +K     D+SSV  +   G+ LD++++  +K +F   K +L  YGMTE  G+++     +    S+G ++ G  VK+VD  T++ LG NQ GE+ VK P+    YI +  +NE     DGFYKTGD+ YYD  G  Y  DR+K+LIKYK  QV P ELE +L++H  + +V V G P+P V EL +A VVK P S ++E ++  +V  A V   K +RG V+FV+ +PK   GKILR++L+D
Sbjct:   64 ASAIKQLGIGKGDIVAICSENRTEYLTTTIAVLCTGATVTFINSAYSKNEFIHTTKISKPKYIFLSPSAYKEFYVTLKKLNIVQKFFLYGISDDK--DVISFRDLATKYTHFNDFRPKDFNGT--DSTSIILYSSGTTGLPKGVKLTHRNLLVSGLQKPFCQKDLVILTLAPWCNTVGIILTLSCLSKNRTVLYLNKFQEDV----YLQCIEKYKAGILLMVPPLLVILTKSKLIDKYDVSSVQIMYCGGAPLDSSVMTSIKKRFTGLKHVLQGYGMTETTGALTEEREHSNKLGSVGKVVEGNIVKVVDVETRKTLGPNQNGEICVKGPVLFEDYIGKD-INE-DLDEDGFYKTGDIAYYDNEGYFYIVDRIKELIKYKAGQVAPSELEAILLQHDAVQDVGVAGAPDPLVGELPTAFVVKKPNSKVTEKELIDFVA-ARVSSWKQLRGGVRFVNEIPKTGSGKILRRILRD 525          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|768409326|ref|XP_011552203.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 220.705 bits (561), Expect = 1.040e-60
Identity = 148/485 (30.52%), Postives = 248/485 (51.13%), Query Frame = 0

Query:  138 LSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLC----YQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQC--TMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTM----FYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE------LGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQD 606
             S++   A   A +  + G+  G+ +     N  +++  + G  L GA+++  S       ++  +  ++PK + C    Y+ + +TI Q+      + L  D   S + L  +   ++       +PV    Q    +IL+SSGTTGLPKG+  +H N L     I    P    T +    +YH  G    +     G  NV    F   +     +E   ++K +++F+     V L+ ++   + D++SVT ++   + LD   ++++K +FPN + ++  YGMTE        ++   +   S+G+      +K+VD  T+E LG NQ GE+  K  +   GYI RP    + F  +GFY++GD+GYYDE G  +  DRLK+LIKYK  QV P E+E  L++HP I +  V G P+ A  EL  A VV  PG+ ++E +++++V    + + K +RG V+FV  +PKN  GKILR++L+D
Sbjct:   50 FSEIAQNAMNVAVSLVQQGVRKGETIAMCSENRSEFWPTVIGTLLAGAVITPISVVYTKDELKHILGISKPKYIFCSPFAYKAHKSTI-QSTKHIRKVYLYGDEKTSNEILYKDLVKNVNVKREDFMPVEVQGQTDDAVILYSSGTTGLPKGVVLTHFNGLVYCSFIKFDEPQDPMTVLSIAPWYHAYGMWGNIGSMCNGRTNVYIKRFEEKL----FLETIQRHKISVLFLVPPILVLLSKSQILKNYDVTSVTQMNCGAAPLDVETIDEVKRRFPNVEGVMQGYGMTESTLALCKDNLFQPYKAGSVGTATPSTVLKVVDLKTREPLGPNQPGEICAKGALIMKGYIGRP--RSEDFDDEGFYRSGDIGYYDEDGYFFIVDRLKELIKYKGFQVPPAEIEAELLKHPAIRDAGVIGVPDKASGELPLAFVVTQPGAAVTEREIQSYVAE-KLSNPKQLRGGVRFVKEIPKNPSGKILRRILRD 526          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|909562920|ref|XP_013136653.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Papilio polytes])

HSP 1 Score: 219.164 bits (557), Expect = 3.483e-60
Identity = 154/482 (31.95%), Postives = 251/482 (52.07%), Query Frame = 0

Query:  145 AFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLC----YQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMG----------EAEPFQ-LPVTQVDQCTMILWSSGTTGLPKGIQHSHRNMLNLVQPIVS-PNPYSYTTTMFYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVE-IMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELG-SISMGHTP---TSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQ 605
            A   A +  K+G++ GDV+     N  +++  + G    G +V+  +++     ++  +  ++PK ++C    Y+      +     + LI    +  ++T  LSY     +G          + + F+ + V   D   MIL+SSGTTGLPKG+  +H N++    P +  P+  + T T +YH  G    + G + G +++    F      VE  +    KYK + + +     V +A+ K     D+SSV  +    + L     + +  +FPN +A+L  YGMTE   ++S+   P    S+G + +   VKIV P T ++LG NQ GE+ VK  +   GYI +    +  F  +GF+KTGD+GYYDE   LY  DRLK+LIKYK  QV P E+E VL++H  + +V V G P P+  E+  A VV  P S ++EA+++ +V    + + K +RG V+F+  +PK+A GKILRK L+
Sbjct:   57 AMNLAVSLVKIGVKRGDVIAICSENRREFWSSVIGVICAGGVVTTINTSYTKDELKHVMGISKPKFVICSPFSYKAQEKNFKNIEGIKNLIIFGEEIPKNT--LSYNALAIVGGSNSMLKENVKIDEFEAIEVVGKDDLAMILYSSGTTGLPKGVMITHLNVVTACSPTMEMPDIVALTITPWYHTMGLMSHLIGFVKGKMSIYLPKFE-----VEQYLRTIEKYKVSQLSVVPP--VLVALCKSPTKHDVSSVMIVVSGAAPLHKETADAVLKRFPNAQAVLQGYGMTEATLAVSINTNPDKKASVGQVTSNSVVKIVHPETGKVLGPNQEGEVRVKGVMLMKGYIGKK--KQDDFDEEGFFKTGDIGYYDEDKYLYIVDRLKELIKYKAYQVAPAEIEAVLLKHEGVRDVGVVGKPHPSAGEVPVAFVVTQPDSKVTEAELQQFVAER-LSNTKHLRGGVRFIDAIPKSATGKILRKDLK 526          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|947081806|gb|KRH30595.1| (hypothetical protein GLYMA_11G194500 [Glycine max])

HSP 1 Score: 218.779 bits (556), Expect = 6.532e-60
Identity = 176/555 (31.71%), Postives = 264/555 (47.57%), Query Frame = 0

Query:   83 ILKAQRPFEPVCLAQDQD---SIIKHIFEKLDDLLADGQGGRPWMTDITEFERPKVQHLSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLCYQ------GNL---ATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQ--CTMILWSSGTTGLPKGIQHSHRN------MLNLVQPIVSPNPYSYTTTM-FYHMGGYHLAVSGAIF-GNVNVVFHGFNHIVTGVEIMEAAHKY--KPNLMFIWTHHGVELAMAKPR--PDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTELGSISMGHTPT-------SMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLL 604
            I ++ RP    CL    D   S++  +F+ +           P    + +    +   L+ +K    K A  F KLG+   DVV    PN + Y I    A   GA+VS  +    V  I  Q++D+ PK+L+          NL   A I    T Q L++    G+E +   S +  ++M      +LP + V Q     +L+SSGTTGL KG+  +HRN      M+ +   +       Y   +  +H+ G  +    A+  G+  VV   F         +EA  K   K  +  +W    + L +AK     + DLSS+  I    + L  +L+E+   +FP+  AI   YGMTE   I     P        S G+L++G E +IV  +TQ+ L   Q+GE+WV+ P    GY N P     +    G+  TGDLGY+DE G LY  DR+K+LIKYK  QV P ELE +L+ HPEI E  V   P+    E+  A VV++P S+L+E +++ ++        KL R  V F++++PK A GKILR+ L
Sbjct:   11 IYRSLRP----CLVLPNDPNLSLVSFLFQSVSSF--------PSKIALVDSHSSQTLTLAHLKSQVAKLAHGFLKLGINKNDVVLLLAPNSIHYPICFLAATAIGAVVSTANPIYTVNEISKQVDDSNPKLLITVPELWDKVKNLNLPAVIIDTETAQGLVSFEA-GNEVSRITSLDAVMEMA-GPATELPESGVKQGDTAALLYSSGTTGLSKGVVLTHRNFIAASVMIGMDDDLAGEQDDVYLCVLPMFHVFGLAVVTYAALRRGSAVVVMERFE--------LEALLKAIEKQRVTKLWVVPPILLGLAKQSVVGNYDLSSLRRIGSGAAPLGKDLMEECGRRFPHV-AICQGYGMTETCGIVSVENPRVGVRHTGSTGTLVSGVEAQIVSVDTQKPLPPRQLGEIWVRGPNMMQGYHNNPEATRLTIDKKGWVHTGDLGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILEAVVVPYPDDEAGEVPIAYVVRSPNSSLTEEEIQKFIAKQVAPFKKLRR--VTFINNVPKTASGKILRREL 540          

BLAST of luciferin 4-monooxygenase vs. nr
Match: gi|768449351|ref|XP_011566756.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 218.009 bits (554), Expect = 8.623e-60
Identity = 147/485 (30.31%), Postives = 247/485 (50.93%), Query Frame = 0

Query:  138 LSQVKPLAFKAASAFTKLGLEAGDVVQFYLPNCVDYYIPLFGAWLCGAIVSLTSSTLKVPAIQSQIEDTQPKVLLC----YQGNLATIEQAITGQTLIALNTDGDESTDHLSYERFLDMGEAEPFQLPVTQVDQC--TMILWSSGTTGLPKGIQHSHRNMLNLVQPIVSPNPYSYTTTM----FYHMGGYHLAVSGAIFGNVNVVFHGFNHIVTGVEIMEAAHKYKPNLMFIWTHHGVELAMAKPRPDLDLSSVTAISPLGSSLDTNLLEDLKPKFPNCKAILNYYGMTE------LGSISMGHTPTSMGSLLNGREVKIVDPNTQELLGANQIGEMWVKTPITTIGYINRPGLNEKSFAPDGFYKTGDLGYYDEAGLLYFHDRLKDLIKYKNNQVYPVELETVLMEHPEIAEVAVYGTPEPAVQELISACVVKTPGSTLSEADVRAWVVHADVDDGKLIRGPVKFVSHLPKNAHGKILRKLLQD 606
             S++   A   A +  + G+  G+ +     N  +++  + G  L GA+++  S       ++  +  ++PK + C    Y+ + +TI Q+      + L  D   S + L  +   ++       +PV    Q    +IL+SSGTTGLPKG+  +H N L     I    P    T +    +YH  G    +     G  NV    F   +     +E   +YK +++F+     V L+ ++   + D++SVT ++   + LD   ++++K +FPN + ++  YGMTE        ++   +   S+G+      +K+VD  T+E LG NQ GE+  K  +   GYI RP    + F  +GFY++GD+GYYDE G  +  DRLK+LIKYK  QV P E+E  L++HP I +  V G P+ A  EL  A VV  PG+ ++E +++++V    + + + +RG  +FV  +PKN  GKILR++L+D
Sbjct:   50 FSEIAQNAMNVAVSLVQQGVRKGETIAVCSENRSEFWPTVIGTLLAGAVITPISVVYTKDELKHILGISKPKYIFCSPFAYKAHKSTI-QSTKHIRKVYLYGDEKTSNEILYKDLVKNVNVKREDFMPVEVQGQTDDAVILYSSGTTGLPKGVVLTHFNGLVYCSFIKFDEPQDPMTVLSIAPWYHAYGMWGNIGSMCNGRTNVYIKRFEEKL----FLETIQRYKISVLFLVPPILVLLSKSQILKNYDVTSVTQMNCGAAPLDVETIDEVKRRFPNVEGVMQGYGMTESTLALCKDNLFQPYKAGSVGTATPSTVLKVVDLKTREPLGPNQPGEICAKGALIMKGYIGRP--RSEDFDDEGFYRSGDIGYYDEDGYFFIVDRLKELIKYKGFQVPPAEIEAELLKHPAIRDAGVVGVPDKASGELPLAFVVTQPGAAVTEREIQSYVAE-KLSNPEQLRGGGRFVKEIPKNPSGKILRRILRD 526